Spg016355 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg016355 vs. NCBI nr
Match: XP_038897147.1 (WD repeat-containing protein PCN-like [Benincasa hispida])

HSP 1 Score: 1567.7 bits (4058), Expect = 0.0e+00
Identity = 769/821 (93.67%), Postives = 789/821 (96.10%), Query Frame = 0

Query: 1   MIEFYRNSSIQWKPSPVVALASSADDSQVAAAREDGSLEIWLVSPGSVGWHCQLTISGDP 60
           MIEFYRNSSI+WKPSPVVALASSADDSQVAAAREDGSLEIWLVSPGSVGWHCQLTISGDP
Sbjct: 1   MIEFYRNSSIEWKPSPVVALASSADDSQVAAAREDGSLEIWLVSPGSVGWHCQLTISGDP 60

Query: 61  TSRVSSLVWCCAGSRDRPSGRLLSSSIDGSISEWDLFDLTQKTVLESIGVSIWQIAAAPC 120
           TSRVSSLVWC AGS+DRP GRL SSSIDGSISEWDLFDLTQK+VLESIGVSIWQIAAAPC
Sbjct: 61  TSRVSSLVWCSAGSKDRPFGRLFSSSIDGSISEWDLFDLTQKSVLESIGVSIWQIAAAPC 120

Query: 121 NIPEIHR-EVKPQDIENGHLTDSESDCQDCSESEDDSNSSEFRVQSSDVNPSLAIACDDG 180
           +  E+HR EV PQD ENGH+TD ESDC    ESEDDSNSSE  VQSSD NPSLAIACDDG
Sbjct: 121 STHEVHREEVNPQDTENGHVTDDESDC----ESEDDSNSSELHVQSSDENPSLAIACDDG 180

Query: 181 CVRIYNICDAEEFVYKRSLPRVSGKVLSVTWSNDAERIFSGSSDGFIRCWNASLGHEIYR 240
           CVRIYNICDAEEF+YKRSLPRVSG+VLSVTWSNDAERIFSGSSDGFIRCWNA+LGHEIYR
Sbjct: 181 CVRIYNICDAEEFIYKRSLPRVSGRVLSVTWSNDAERIFSGSSDGFIRCWNANLGHEIYR 240

Query: 241 ITAGLGGLGSGPELCVWSLLSLRCGTLVSADSSGSVQFWDSNHGTLLQAHTLHKGDVNAL 300
           ITAGLGGLGS PELCVWSLLSLRCGTLVSADS+GSVQFWDSNHGTLLQAHTLHKGDVNAL
Sbjct: 241 ITAGLGGLGSEPELCVWSLLSLRCGTLVSADSTGSVQFWDSNHGTLLQAHTLHKGDVNAL 300

Query: 301 AVTPNHNRVYSAGSDGQVILYKLSNENVASSEDQNSSEVMKKWIYVGHVRAHTHDIRALT 360
           A TPNHNRVYSAGSDGQVILYKLSNENVASSED+ SSE MKKWIYVGHVRAHTHDIRALT
Sbjct: 301 AATPNHNRVYSAGSDGQVILYKLSNENVASSEDKCSSETMKKWIYVGHVRAHTHDIRALT 360

Query: 361 VAVPICREAELSQDEEVKRVRYKKKPNDFSYRKWAHLGVPMLISGGDDTKLFAYSAQEFT 420
           VAVPICRE E SQDEEVKR+RYKKKPNDFSYRKWAHLGVPML+SGGDDTKLFAYSAQEFT
Sbjct: 361 VAVPICRE-EPSQDEEVKRIRYKKKPNDFSYRKWAHLGVPMLMSGGDDTKLFAYSAQEFT 420

Query: 421 KFSPHDICPAPQRVPMQLVLNTVFNQAPLLLVQAAHSLDILCVRPKSGSFRDMACGPSKG 480
           KFSPHDICPAPQRVPMQLVLNTVFNQAPLLLVQAAHSL+ILC+RPKSGSF D ACGPSKG
Sbjct: 421 KFSPHDICPAPQRVPMQLVLNTVFNQAPLLLVQAAHSLEILCIRPKSGSFGDKACGPSKG 480

Query: 481 LAKADLLVRVKSKASRKIICSTISNSGKLFAYSDHTKPNLFELKKSGGSKCSWTVNRRQL 540
             KADLLVRVKSKASRKIICSTISNSGKLFAYSDHTKPNLFELKKSGGSKCSWTV RR+L
Sbjct: 481 QTKADLLVRVKSKASRKIICSTISNSGKLFAYSDHTKPNLFELKKSGGSKCSWTVIRRKL 540

Query: 541 PNTLPFAHSMVFSFDSSRLIIAGHDKRIYVVDVGSLEVIHSFTPFRELQDDTLPPAEPPI 600
           PN LPFAHSMVFSFDSSRLIIAGHDKRIYVVDVGSLEV+HSFTPFRELQD TLPP EPPI
Sbjct: 541 PNMLPFAHSMVFSFDSSRLIIAGHDKRIYVVDVGSLEVLHSFTPFRELQDATLPPTEPPI 600

Query: 601 TKIFTSSDGQWLAAVNCFGDIYIFNMEIMRQHWFISRLDGASVTAGGFPQWNNNVLVVTT 660
           TK+FTSSDGQWLAAVNCFGDIY+FNMEIMRQHWFISRLDGAS+TAGGFPQWNNNVLVVTT
Sbjct: 601 TKLFTSSDGQWLAAVNCFGDIYVFNMEIMRQHWFISRLDGASITAGGFPQWNNNVLVVTT 660

Query: 661 SSNQVYAFDVEAKQLGDWSMRHTQALPKRFQEFPGEVIGLSFPPSATSLPVIVYSSRAMC 720
           SSNQVYAFDVEAKQLGDWSMRHTQALPKRFQEFPGEVIGLSFPPSATSLPVIVYSSRAMC
Sbjct: 661 SSNQVYAFDVEAKQLGDWSMRHTQALPKRFQEFPGEVIGLSFPPSATSLPVIVYSSRAMC 720

Query: 721 LIDFRMSVDQDDEYVMISGQDSTVKSLRGTPINGKLKRKLRDCQTEGRLHGRKNFEIFPF 780
           LIDFRMSVDQDDEYVMISGQDSTVKSL GTPINGKLKRKLRDCQTEGR HGRKNFEIFPF
Sbjct: 721 LIDFRMSVDQDDEYVMISGQDSTVKSLWGTPINGKLKRKLRDCQTEGRPHGRKNFEIFPF 780

Query: 781 RDPVLLIGHLSKTSLLIIEKPWLEVANTFDAPPVHRHIYGT 821
           RDPVLLIGHLSKTSLLIIEKPWLEVANTFDAPPVHRHIYGT
Sbjct: 781 RDPVLLIGHLSKTSLLIIEKPWLEVANTFDAPPVHRHIYGT 816

BLAST of Spg016355 vs. NCBI nr
Match: XP_023535891.1 (U3 small nucleolar RNA-associated protein 4 homolog isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1559.3 bits (4036), Expect = 0.0e+00
Identity = 754/820 (91.95%), Postives = 787/820 (95.98%), Query Frame = 0

Query: 1   MIEFYRNSSIQWKPSPVVALASSADDSQVAAAREDGSLEIWLVSPGSVGWHCQLTISGDP 60
           MIEFYRNSSI+WKPSPVVALASSAD SQVAAAREDGSLEIWLVSPGSVGWHCQLTI+GDP
Sbjct: 1   MIEFYRNSSIEWKPSPVVALASSADGSQVAAAREDGSLEIWLVSPGSVGWHCQLTINGDP 60

Query: 61  TSRVSSLVWCCAGSRDRPSGRLLSSSIDGSISEWDLFDLTQKTVLESIGVSIWQIAAAPC 120
           TSRVSSLVWC AG +DRPSGRL SSSIDGSISEWDLF LTQK+VLESIG+SIWQIAAAPC
Sbjct: 61  TSRVSSLVWCRAGLKDRPSGRLFSSSIDGSISEWDLFGLTQKSVLESIGLSIWQIAAAPC 120

Query: 121 NIPEIHREVKPQDIENGHLTDSESDCQDCSESEDDSNSSEFRVQSSDVNPSLAIACDDGC 180
           N  E+HREVKP++IEN H+TD+ESDCQDCSESEDDSNSSE  VQ+SD + SLAIACDDGC
Sbjct: 121 NSREVHREVKPKEIENAHVTDNESDCQDCSESEDDSNSSELHVQASDEDHSLAIACDDGC 180

Query: 181 VRIYNICDAEEFVYKRSLPRVSGKVLSVTWSNDAERIFSGSSDGFIRCWNASLGHEIYRI 240
           VRIYNICDAEE +YKRSLPRVSG+VLSVTWSNDAERIFSGSSDGFIRCWNASLGHEIYRI
Sbjct: 181 VRIYNICDAEELIYKRSLPRVSGRVLSVTWSNDAERIFSGSSDGFIRCWNASLGHEIYRI 240

Query: 241 TAGLGGLGSGPELCVWSLLSLRCGTLVSADSSGSVQFWDSNHGTLLQAHTLHKGDVNALA 300
           TAGLGGLGSG ELCVWSLL LRCGTLVSADS+GSVQFWDSNHGTLLQAHTLHKGDVNALA
Sbjct: 241 TAGLGGLGSGSELCVWSLLFLRCGTLVSADSTGSVQFWDSNHGTLLQAHTLHKGDVNALA 300

Query: 301 VTPNHNRVYSAGSDGQVILYKLSNENVASSEDQNSSEVMKKWIYVGHVRAHTHDIRALTV 360
            TPNHNRVYSAGSDGQVILY+LSNENV SSED+ SSE+MKKWIYVGHVRAHTHDIRALTV
Sbjct: 301 ATPNHNRVYSAGSDGQVILYRLSNENVVSSEDKYSSEIMKKWIYVGHVRAHTHDIRALTV 360

Query: 361 AVPICREAELSQDEEVKRVRYKKKPNDFSYRKWAHLGVPMLISGGDDTKLFAYSAQEFTK 420
           AVPICREAE SQD EVKR+R+K+KPNDFSY KWAHLGVPML+SGGDDTKLFAYSAQEFTK
Sbjct: 361 AVPICREAEASQDVEVKRIRHKRKPNDFSYHKWAHLGVPMLMSGGDDTKLFAYSAQEFTK 420

Query: 421 FSPHDICPAPQRVPMQLVLNTVFNQAPLLLVQAAHSLDILCVRPKSGSFRDMACGPSKGL 480
           FSPHDICPAPQRVPMQLVLNTVFNQAPLLLVQAAHSLDILCVRPK GSF D  CGPSKGL
Sbjct: 421 FSPHDICPAPQRVPMQLVLNTVFNQAPLLLVQAAHSLDILCVRPKCGSFGDKTCGPSKGL 480

Query: 481 AKADLLVRVKSKASRKIICSTISNSGKLFAYSDHTKPNLFELKKSGGSKCSWTVNRRQLP 540
           AKADLLVRVKSKASRKIICSTISNSGKLFA+SDHTKPNL+ELKKSGGSKCSWTVNRR+LP
Sbjct: 481 AKADLLVRVKSKASRKIICSTISNSGKLFAFSDHTKPNLYELKKSGGSKCSWTVNRRKLP 540

Query: 541 NTLPFAHSMVFSFDSSRLIIAGHDKRIYVVDVGSLEVIHSFTPFRELQDDTLPPAEPPIT 600
           NTLPFAHSMVFSFDSSRLIIAGHDKRIYVVDV SLEV+HSFTPFRE QDD LPPAEPPIT
Sbjct: 541 NTLPFAHSMVFSFDSSRLIIAGHDKRIYVVDVSSLEVLHSFTPFREQQDDILPPAEPPIT 600

Query: 601 KIFTSSDGQWLAAVNCFGDIYIFNMEIMRQHWFISRLDGASVTAGGFPQWNNNVLVVTTS 660
           KIFTSSDGQWLAAVNCFGDIY+FNMEI+RQHWFISRLDGAS+TAGGFPQWNNNVLVVTTS
Sbjct: 601 KIFTSSDGQWLAAVNCFGDIYVFNMEILRQHWFISRLDGASITAGGFPQWNNNVLVVTTS 660

Query: 661 SNQVYAFDVEAKQLGDWSMRHTQALPKRFQEFPGEVIGLSFPPSATSLPVIVYSSRAMCL 720
           SNQ+YAFD+EAKQLGDWSMRHTQALPKRFQEFPGEVIGLSFPPS TSLPVIVYSSRAMCL
Sbjct: 661 SNQIYAFDIEAKQLGDWSMRHTQALPKRFQEFPGEVIGLSFPPSTTSLPVIVYSSRAMCL 720

Query: 721 IDFRMSVDQDDEYVMISGQDSTVKSLRGTPINGKLKRKLRDCQTEGRLHGRKNFEIFPFR 780
           IDFRMSVDQ+DEYVMISGQDSTVK+L GTPINGKLKRKLRDCQTEGR HGRKNFE FPFR
Sbjct: 721 IDFRMSVDQNDEYVMISGQDSTVKNLWGTPINGKLKRKLRDCQTEGRPHGRKNFENFPFR 780

Query: 781 DPVLLIGHLSKTSLLIIEKPWLEVANTFDAPPVHRHIYGT 821
           DPVLLIGHLSKTSLLIIEKPWLEV NTFDAPPVHRHIYGT
Sbjct: 781 DPVLLIGHLSKTSLLIIEKPWLEVVNTFDAPPVHRHIYGT 820

BLAST of Spg016355 vs. NCBI nr
Match: XP_022936102.1 (U3 small nucleolar RNA-associated protein 4 homolog isoform X2 [Cucurbita moschata])

HSP 1 Score: 1557.7 bits (4032), Expect = 0.0e+00
Identity = 752/820 (91.71%), Postives = 786/820 (95.85%), Query Frame = 0

Query: 1   MIEFYRNSSIQWKPSPVVALASSADDSQVAAAREDGSLEIWLVSPGSVGWHCQLTISGDP 60
           MIEFYRNSSI+WKPSPVVALASSAD SQVAAAREDGSLEIWLVSPGSVGWHCQLTI+GDP
Sbjct: 1   MIEFYRNSSIEWKPSPVVALASSADGSQVAAAREDGSLEIWLVSPGSVGWHCQLTINGDP 60

Query: 61  TSRVSSLVWCCAGSRDRPSGRLLSSSIDGSISEWDLFDLTQKTVLESIGVSIWQIAAAPC 120
           TSRVSSLVWC AGS+DRPSGRL SSSIDGSISEWDLF LTQK+VLESIG+SIWQIAAAPC
Sbjct: 61  TSRVSSLVWCRAGSKDRPSGRLFSSSIDGSISEWDLFGLTQKSVLESIGLSIWQIAAAPC 120

Query: 121 NIPEIHREVKPQDIENGHLTDSESDCQDCSESEDDSNSSEFRVQSSDVNPSLAIACDDGC 180
           N  E+HREVKP++IEN H+TD+ESDCQDCSESEDDSNSS+  VQ+SD +P LAIACDDGC
Sbjct: 121 NSREVHREVKPKEIENAHVTDNESDCQDCSESEDDSNSSKLHVQASDEDPCLAIACDDGC 180

Query: 181 VRIYNICDAEEFVYKRSLPRVSGKVLSVTWSNDAERIFSGSSDGFIRCWNASLGHEIYRI 240
           VRIYNICD EE +YKRSLPRVSG+VLSVTWSNDAERIFSGSSDGFIRCWNASLGHE+YRI
Sbjct: 181 VRIYNICDVEELIYKRSLPRVSGRVLSVTWSNDAERIFSGSSDGFIRCWNASLGHEVYRI 240

Query: 241 TAGLGGLGSGPELCVWSLLSLRCGTLVSADSSGSVQFWDSNHGTLLQAHTLHKGDVNALA 300
           TAGLGGLGSG ELCVWSLL LRCGTLVSADS+GSVQFWDSNHGTLLQAHTLHKGDVNALA
Sbjct: 241 TAGLGGLGSGSELCVWSLLFLRCGTLVSADSTGSVQFWDSNHGTLLQAHTLHKGDVNALA 300

Query: 301 VTPNHNRVYSAGSDGQVILYKLSNENVASSEDQNSSEVMKKWIYVGHVRAHTHDIRALTV 360
            TPNHNRVYSAGSDGQVILYKLSNENV SSED+ SSE+MKKWIYVGHVRAHTHDIRALTV
Sbjct: 301 ATPNHNRVYSAGSDGQVILYKLSNENVLSSEDKYSSEMMKKWIYVGHVRAHTHDIRALTV 360

Query: 361 AVPICREAELSQDEEVKRVRYKKKPNDFSYRKWAHLGVPMLISGGDDTKLFAYSAQEFTK 420
           AVPICREAE SQD EVKR+R+K+KPNDFSY KWAHLGVPML+SGGDDTKLFAYSAQEFTK
Sbjct: 361 AVPICREAEASQDVEVKRIRHKRKPNDFSYHKWAHLGVPMLMSGGDDTKLFAYSAQEFTK 420

Query: 421 FSPHDICPAPQRVPMQLVLNTVFNQAPLLLVQAAHSLDILCVRPKSGSFRDMACGPSKGL 480
           FSPHDICPAPQRVPMQLVLNTVFNQAPLLLVQAAHSLDILCVRPK GSF D  CGPSKGL
Sbjct: 421 FSPHDICPAPQRVPMQLVLNTVFNQAPLLLVQAAHSLDILCVRPKCGSFGDKTCGPSKGL 480

Query: 481 AKADLLVRVKSKASRKIICSTISNSGKLFAYSDHTKPNLFELKKSGGSKCSWTVNRRQLP 540
           AKADLLVRVKSKASRKIICSTISNSGKLFA+SDH KPNL+ELKKSGGSKCSWTVNRR+LP
Sbjct: 481 AKADLLVRVKSKASRKIICSTISNSGKLFAFSDHAKPNLYELKKSGGSKCSWTVNRRKLP 540

Query: 541 NTLPFAHSMVFSFDSSRLIIAGHDKRIYVVDVGSLEVIHSFTPFRELQDDTLPPAEPPIT 600
           NTLPFAHSMVFSFDSSRLIIAGHDKRIYVVDV SLEV+HSFTPFRE QDD LPPAEPPIT
Sbjct: 541 NTLPFAHSMVFSFDSSRLIIAGHDKRIYVVDVSSLEVLHSFTPFREQQDDILPPAEPPIT 600

Query: 601 KIFTSSDGQWLAAVNCFGDIYIFNMEIMRQHWFISRLDGASVTAGGFPQWNNNVLVVTTS 660
           KIFTSSDGQWLAAVNCFGDIY+FNMEI+RQHWFISRLDGAS+TAGGFPQWNNNVLVVTTS
Sbjct: 601 KIFTSSDGQWLAAVNCFGDIYVFNMEILRQHWFISRLDGASITAGGFPQWNNNVLVVTTS 660

Query: 661 SNQVYAFDVEAKQLGDWSMRHTQALPKRFQEFPGEVIGLSFPPSATSLPVIVYSSRAMCL 720
           SNQ+YAFD+EAKQLGDWSMRHTQALPKRFQEFPGEVIGLSFPPS TSLPVIVYSSRAMCL
Sbjct: 661 SNQIYAFDIEAKQLGDWSMRHTQALPKRFQEFPGEVIGLSFPPSTTSLPVIVYSSRAMCL 720

Query: 721 IDFRMSVDQDDEYVMISGQDSTVKSLRGTPINGKLKRKLRDCQTEGRLHGRKNFEIFPFR 780
           IDFRMSVDQ+DEYVMISGQDSTVK+L GTPINGKLKRKLRDCQTEGR HGRKNFE FPFR
Sbjct: 721 IDFRMSVDQNDEYVMISGQDSTVKNLWGTPINGKLKRKLRDCQTEGRPHGRKNFENFPFR 780

Query: 781 DPVLLIGHLSKTSLLIIEKPWLEVANTFDAPPVHRHIYGT 821
           DPVLLIGHLSKTSLLIIEKPWLEV NTFDAPPVHRHIYGT
Sbjct: 781 DPVLLIGHLSKTSLLIIEKPWLEVVNTFDAPPVHRHIYGT 820

BLAST of Spg016355 vs. NCBI nr
Match: KAG6591430.1 (WD repeat-containing protein PCN, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1555.0 bits (4025), Expect = 0.0e+00
Identity = 753/821 (91.72%), Postives = 787/821 (95.86%), Query Frame = 0

Query: 1   MIEFYRNSSIQWKPSPVVALASSADDSQVAAAREDGSLEIWLVSPGSVGWHCQLTISGDP 60
           MIEFYRNSSI+WKPSPVVALASSAD SQVAAAREDGSLEIWLVSPGSVGWHCQLTI+GDP
Sbjct: 1   MIEFYRNSSIEWKPSPVVALASSADGSQVAAAREDGSLEIWLVSPGSVGWHCQLTINGDP 60

Query: 61  TSRVSSLVWCCAGSRDRPSGRLLSSSIDGSISEWDLFDLTQKTVLESIGVSIWQIAAAPC 120
           TSRVSSLVWC AGS+DRPSGRL SSSIDGSISEWDLF LTQK+VLESIG+SIWQIAAAPC
Sbjct: 61  TSRVSSLVWCRAGSKDRPSGRLFSSSIDGSISEWDLFGLTQKSVLESIGLSIWQIAAAPC 120

Query: 121 NIPEIHREVKPQDIENGHLTDSESDCQDCSESEDDSNSSEFRVQSSDVNPSLAIACDDGC 180
           N  E+HREVKP++IEN H+TD+ESDCQDCSESEDDSNSS+  VQ+SD +P LAIACDDGC
Sbjct: 121 NSREVHREVKPKEIENAHVTDNESDCQDCSESEDDSNSSKLHVQASDEDPCLAIACDDGC 180

Query: 181 VRIYNICDAEEFVYKRSLPRVSGKVLSVTWSNDAERIFSGSSDGFIRCWNASLGHEIYRI 240
           VRIYNICDAEE +YKRSLPRVSG+VLSVTWSNDAERIFSGSSDGFIRCWNASLGHE+YRI
Sbjct: 181 VRIYNICDAEELIYKRSLPRVSGRVLSVTWSNDAERIFSGSSDGFIRCWNASLGHEVYRI 240

Query: 241 TAGLGGLGSGPELCVWSLLSLRCGTLVSADSSGSVQFWDSNHGTLLQAHTLHKGDVNALA 300
           TAGLGGLGSG ELCVWSLL LRCGTLVSADS+GSVQFWDSNHGTLLQAHTLHKGDVNALA
Sbjct: 241 TAGLGGLGSGSELCVWSLLFLRCGTLVSADSTGSVQFWDSNHGTLLQAHTLHKGDVNALA 300

Query: 301 VTPNHNRVYSAGSDGQVILYKLSNENVASSEDQNSSEVMKKWIYVGHVRAHTHDIRALTV 360
            TPNHNRVYSAGSDGQVILYKLSNENV SSED+ SSE+MKKWIYVGHVRAHTHDIRALTV
Sbjct: 301 ATPNHNRVYSAGSDGQVILYKLSNENVVSSEDKYSSEMMKKWIYVGHVRAHTHDIRALTV 360

Query: 361 AVPICRE-AELSQDEEVKRVRYKKKPNDFSYRKWAHLGVPMLISGGDDTKLFAYSAQEFT 420
           AVPICRE AE SQD EVKR+R+K+KPNDFSY KWAHLGVPML+SGGDDTKLFAYSAQEFT
Sbjct: 361 AVPICREAAEASQDVEVKRIRHKRKPNDFSYHKWAHLGVPMLMSGGDDTKLFAYSAQEFT 420

Query: 421 KFSPHDICPAPQRVPMQLVLNTVFNQAPLLLVQAAHSLDILCVRPKSGSFRDMACGPSKG 480
           KFSPHDICPAPQRVPMQLVLNTVFNQAPLLLVQAAHSLDILCVRPK GSF D  CGPSKG
Sbjct: 421 KFSPHDICPAPQRVPMQLVLNTVFNQAPLLLVQAAHSLDILCVRPKCGSFGDKTCGPSKG 480

Query: 481 LAKADLLVRVKSKASRKIICSTISNSGKLFAYSDHTKPNLFELKKSGGSKCSWTVNRRQL 540
           LAKADLLVRVKSKASRKIICSTISNSGKLFA+SDH KPNL+ELKKSGGSKCSWTVNRR+L
Sbjct: 481 LAKADLLVRVKSKASRKIICSTISNSGKLFAFSDHAKPNLYELKKSGGSKCSWTVNRRKL 540

Query: 541 PNTLPFAHSMVFSFDSSRLIIAGHDKRIYVVDVGSLEVIHSFTPFRELQDDTLPPAEPPI 600
           PNTLPFAHSMVFSFDSSRLIIAGHDKRIYVVDV SLEV+HSFTPFRE QDD LPPAEPPI
Sbjct: 541 PNTLPFAHSMVFSFDSSRLIIAGHDKRIYVVDVSSLEVLHSFTPFREQQDDILPPAEPPI 600

Query: 601 TKIFTSSDGQWLAAVNCFGDIYIFNMEIMRQHWFISRLDGASVTAGGFPQWNNNVLVVTT 660
           TKIFTSSDGQWLAAVNCFGDIY+FNMEI+RQHWFISRLDGAS+TAGGFPQWNNNVLVVTT
Sbjct: 601 TKIFTSSDGQWLAAVNCFGDIYVFNMEILRQHWFISRLDGASITAGGFPQWNNNVLVVTT 660

Query: 661 SSNQVYAFDVEAKQLGDWSMRHTQALPKRFQEFPGEVIGLSFPPSATSLPVIVYSSRAMC 720
           SSNQ+YAFD+EAKQLGDWSMRHTQALPKRFQEFPGEVIGLSFPPS TSLPVIVYSSRAMC
Sbjct: 661 SSNQIYAFDIEAKQLGDWSMRHTQALPKRFQEFPGEVIGLSFPPSTTSLPVIVYSSRAMC 720

Query: 721 LIDFRMSVDQDDEYVMISGQDSTVKSLRGTPINGKLKRKLRDCQTEGRLHGRKNFEIFPF 780
           LIDFRMSVDQ+DEYVMISGQDSTVK+L GTPINGKLKRKLRDCQTEGR HGRKNFE FPF
Sbjct: 721 LIDFRMSVDQNDEYVMISGQDSTVKNLWGTPINGKLKRKLRDCQTEGRPHGRKNFENFPF 780

Query: 781 RDPVLLIGHLSKTSLLIIEKPWLEVANTFDAPPVHRHIYGT 821
           RDPVLLIGHLSKTSLLIIEKPWLEV NTFDAPPVHRHIYGT
Sbjct: 781 RDPVLLIGHLSKTSLLIIEKPWLEVVNTFDAPPVHRHIYGT 821

BLAST of Spg016355 vs. NCBI nr
Match: XP_023535890.1 (U3 small nucleolar RNA-associated protein 4 homolog isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1554.7 bits (4024), Expect = 0.0e+00
Identity = 754/821 (91.84%), Postives = 787/821 (95.86%), Query Frame = 0

Query: 1   MIEFYRNSSIQWKPSPVVALASSADDSQVAAAREDGSLEIWLVSPGSVGWHCQLTISGDP 60
           MIEFYRNSSI+WKPSPVVALASSAD SQVAAAREDGSLEIWLVSPGSVGWHCQLTI+GDP
Sbjct: 1   MIEFYRNSSIEWKPSPVVALASSADGSQVAAAREDGSLEIWLVSPGSVGWHCQLTINGDP 60

Query: 61  TSRVSSLVWCCAGSRDRPSGRLLSSSIDGSISEWDLFDLTQKTVLESIGVSIWQIAAAPC 120
           TSRVSSLVWC AG +DRPSGRL SSSIDGSISEWDLF LTQK+VLESIG+SIWQIAAAPC
Sbjct: 61  TSRVSSLVWCRAGLKDRPSGRLFSSSIDGSISEWDLFGLTQKSVLESIGLSIWQIAAAPC 120

Query: 121 NIPEIHREVKPQDIENGHLTDSESDCQDCSESEDDSNSSEFRVQSSDVNPSLAIACDDGC 180
           N  E+HREVKP++IEN H+TD+ESDCQDCSESEDDSNSSE  VQ+SD + SLAIACDDGC
Sbjct: 121 NSREVHREVKPKEIENAHVTDNESDCQDCSESEDDSNSSELHVQASDEDHSLAIACDDGC 180

Query: 181 VRIYNICDAEEFVYKRSLPRVSGKVLSVTWSNDAERIFSGSSDGFIRCWNASLGHEIYRI 240
           VRIYNICDAEE +YKRSLPRVSG+VLSVTWSNDAERIFSGSSDGFIRCWNASLGHEIYRI
Sbjct: 181 VRIYNICDAEELIYKRSLPRVSGRVLSVTWSNDAERIFSGSSDGFIRCWNASLGHEIYRI 240

Query: 241 TAGLGGLGSGPELCVWSLLSLRCGTLVSADSSGSVQFWDSNHGTLLQAHTLHKGDVNALA 300
           TAGLGGLGSG ELCVWSLL LRCGTLVSADS+GSVQFWDSNHGTLLQAHTLHKGDVNALA
Sbjct: 241 TAGLGGLGSGSELCVWSLLFLRCGTLVSADSTGSVQFWDSNHGTLLQAHTLHKGDVNALA 300

Query: 301 VTPNHNRVYSAGSDGQVILYKLSNENVASSEDQNSSEVMKKWIYVGHVRAHTHDIRALTV 360
            TPNHNRVYSAGSDGQVILY+LSNENV SSED+ SSE+MKKWIYVGHVRAHTHDIRALTV
Sbjct: 301 ATPNHNRVYSAGSDGQVILYRLSNENVVSSEDKYSSEIMKKWIYVGHVRAHTHDIRALTV 360

Query: 361 AVPICRE-AELSQDEEVKRVRYKKKPNDFSYRKWAHLGVPMLISGGDDTKLFAYSAQEFT 420
           AVPICRE AE SQD EVKR+R+K+KPNDFSY KWAHLGVPML+SGGDDTKLFAYSAQEFT
Sbjct: 361 AVPICREAAEASQDVEVKRIRHKRKPNDFSYHKWAHLGVPMLMSGGDDTKLFAYSAQEFT 420

Query: 421 KFSPHDICPAPQRVPMQLVLNTVFNQAPLLLVQAAHSLDILCVRPKSGSFRDMACGPSKG 480
           KFSPHDICPAPQRVPMQLVLNTVFNQAPLLLVQAAHSLDILCVRPK GSF D  CGPSKG
Sbjct: 421 KFSPHDICPAPQRVPMQLVLNTVFNQAPLLLVQAAHSLDILCVRPKCGSFGDKTCGPSKG 480

Query: 481 LAKADLLVRVKSKASRKIICSTISNSGKLFAYSDHTKPNLFELKKSGGSKCSWTVNRRQL 540
           LAKADLLVRVKSKASRKIICSTISNSGKLFA+SDHTKPNL+ELKKSGGSKCSWTVNRR+L
Sbjct: 481 LAKADLLVRVKSKASRKIICSTISNSGKLFAFSDHTKPNLYELKKSGGSKCSWTVNRRKL 540

Query: 541 PNTLPFAHSMVFSFDSSRLIIAGHDKRIYVVDVGSLEVIHSFTPFRELQDDTLPPAEPPI 600
           PNTLPFAHSMVFSFDSSRLIIAGHDKRIYVVDV SLEV+HSFTPFRE QDD LPPAEPPI
Sbjct: 541 PNTLPFAHSMVFSFDSSRLIIAGHDKRIYVVDVSSLEVLHSFTPFREQQDDILPPAEPPI 600

Query: 601 TKIFTSSDGQWLAAVNCFGDIYIFNMEIMRQHWFISRLDGASVTAGGFPQWNNNVLVVTT 660
           TKIFTSSDGQWLAAVNCFGDIY+FNMEI+RQHWFISRLDGAS+TAGGFPQWNNNVLVVTT
Sbjct: 601 TKIFTSSDGQWLAAVNCFGDIYVFNMEILRQHWFISRLDGASITAGGFPQWNNNVLVVTT 660

Query: 661 SSNQVYAFDVEAKQLGDWSMRHTQALPKRFQEFPGEVIGLSFPPSATSLPVIVYSSRAMC 720
           SSNQ+YAFD+EAKQLGDWSMRHTQALPKRFQEFPGEVIGLSFPPS TSLPVIVYSSRAMC
Sbjct: 661 SSNQIYAFDIEAKQLGDWSMRHTQALPKRFQEFPGEVIGLSFPPSTTSLPVIVYSSRAMC 720

Query: 721 LIDFRMSVDQDDEYVMISGQDSTVKSLRGTPINGKLKRKLRDCQTEGRLHGRKNFEIFPF 780
           LIDFRMSVDQ+DEYVMISGQDSTVK+L GTPINGKLKRKLRDCQTEGR HGRKNFE FPF
Sbjct: 721 LIDFRMSVDQNDEYVMISGQDSTVKNLWGTPINGKLKRKLRDCQTEGRPHGRKNFENFPF 780

Query: 781 RDPVLLIGHLSKTSLLIIEKPWLEVANTFDAPPVHRHIYGT 821
           RDPVLLIGHLSKTSLLIIEKPWLEV NTFDAPPVHRHIYGT
Sbjct: 781 RDPVLLIGHLSKTSLLIIEKPWLEVVNTFDAPPVHRHIYGT 821

BLAST of Spg016355 vs. ExPASy Swiss-Prot
Match: Q8RXU6 (WD repeat-containing protein PCN OS=Arabidopsis thaliana OX=3702 GN=PCN PE=2 SV=1)

HSP 1 Score: 1047.7 bits (2708), Expect = 6.7e-305
Identity = 531/830 (63.98%), Postives = 636/830 (76.63%), Query Frame = 0

Query: 5   YRNSSIQWKPSPVVALASSADDSQVAAAREDGSLEIWLVSPGSVGWHCQLTISGDPTSRV 64
           YR SS+ WKPSPVVALA+S+DDSQVAAAREDGSLEIWLVSPG+VGWHCQLTI GDP SR+
Sbjct: 4   YRCSSVDWKPSPVVALANSSDDSQVAAAREDGSLEIWLVSPGAVGWHCQLTIHGDPNSRI 63

Query: 65  SSLVWCCAGSRDRPSGRLLSSSIDGSISEWDLFDLTQKTVLESIGVSIWQIAAAPCNIPE 124
           SSL WCC+ S   PSGRL SSSIDGSISEWDLFDL QK VLESIG+SIWQ+A AP  I  
Sbjct: 64  SSLAWCCSPSIGLPSGRLFSSSIDGSISEWDLFDLKQKIVLESIGISIWQMALAP--ISG 123

Query: 125 IHREVKPQDIENGHLTDSESDCQDCSESEDDSNSSEFRVQS-SDVNPSLAIACDDGCVRI 184
              +V  + I+NG+L++  +D ++    ED S+S EF  +S  +++  LA ACDDGCVR+
Sbjct: 124 FSSDV--EGIKNGYLSEKSNDEEEIGSEEDGSDSDEFHEKSEEEIDRILAAACDDGCVRL 183

Query: 185 YNICDAEEFVYKRSLPRVSGKVLSVTWSNDAERIFSGSSDGFIRCWNASLGHEIYRITAG 244
           Y I + E+  Y RSLPRVSG+ LSVTWS DA+RIFSGSSDG IRCW+A+  HE+YRITAG
Sbjct: 184 YRISNLEKLTYYRSLPRVSGRALSVTWSPDAKRIFSGSSDGLIRCWDATSCHEVYRITAG 243

Query: 245 LGGLGSGPELCVWSLLSLRCGTLVSADSSGSVQFWDSNHGTLLQAHTLHKGDVNALAVTP 304
           LGGLGS  E+CVWSLLSLRC  LVS DS+G+VQFWDS HGTLL+AH+ HKGDVN LA  P
Sbjct: 244 LGGLGSSSEICVWSLLSLRCSVLVSGDSTGTVQFWDSEHGTLLEAHSNHKGDVNTLAAAP 303

Query: 305 NHNRVYSAGSDGQVILYKLSNENVASSEDQNSSEVMKKWIYVGHVRAHTHDIRALTVAVP 364
           +HNRV+SAG+DGQVILYKLS     S + + SS   +KW Y+G+V+AHTHDIRALTVAVP
Sbjct: 304 SHNRVFSAGADGQVILYKLSGSTNGSQDLKPSSS--QKWDYIGYVKAHTHDIRALTVAVP 363

Query: 365 ICRE----AELSQDEEVKRVRYKKKPNDFSYRKWAHLGVPMLISGGDDTKLFAYSAQEFT 424
           I RE     ++  D+  ++ R K KP DF+Y KWAHLGVPMLIS GDD KLFAYS QEFT
Sbjct: 364 ISREDPFPDDILPDKASRKHRKKGKPVDFTYHKWAHLGVPMLISAGDDAKLFAYSIQEFT 423

Query: 425 KFSPHDICPAPQRVPMQLVLNTVFNQAPLLLVQAAHSLDILCVRPKSGSFRDMACGPSKG 484
           KFSPHDICPAPQR+PMQ+V N++FN+  LLLVQ   +LDIL +   S          S G
Sbjct: 424 KFSPHDICPAPQRIPMQMVHNSMFNKTSLLLVQGISTLDILRLNISS---------DSSG 483

Query: 485 LAKADLLVRVKSKASRKIICSTISNSGKLFAYSDHTKPNLFELKKSGGSKCSWTVNRRQL 544
            A    LVRVKS+ +RKIICS ISN+G  FAYSD   P+LFELKK+  +KC W+V+RR+L
Sbjct: 484 RASTKSLVRVKSRDARKIICSAISNTGSHFAYSDQIGPSLFELKKNEFTKCPWSVSRRRL 543

Query: 545 PNTLPFAHSMVFSFDSSRLIIAGHDKRIYVVDVGSLEVIHSFTPFRELQDDTLP-PAEPP 604
           P  LPFAHSM+FS D SRLIIAGHD+RIY +D+ SLE++++FTP RE  +   P P EPP
Sbjct: 544 PE-LPFAHSMIFSSDCSRLIIAGHDRRIYTIDISSLELVYAFTPSREEHEGEAPTPKEPP 603

Query: 605 ITKIFTSSDGQWLAAVNCFGDIYIFNMEIMRQHWFISRLDGASVTAGGFPQWNNNVLVVT 664
           ITK+FTSSDGQWLAA+NCFGDIY+FN+E  RQHWFISRLDGASVTA GF  WNNN LV++
Sbjct: 604 ITKLFTSSDGQWLAAINCFGDIYVFNLETQRQHWFISRLDGASVTAAGFHPWNNNALVIS 663

Query: 665 TSSNQVYAFDVEAKQLGDWSMRHTQALPKRFQEFPGEVIGLSFPPSATSLPVIVYSSRAM 724
           TSSNQV+AFDVEA+QLG WS+ +T  LPKR+QEFPGEV+GLSF PS  S  VIVYSSRA 
Sbjct: 664 TSSNQVFAFDVEARQLGKWSLLNTYVLPKRYQEFPGEVLGLSFSPSPNSSSVIVYSSRAK 723

Query: 725 CLIDFRMSVDQDDEYVMISGQDSTVKSLRGTPIN-----GK---LKRKLRDCQTEGRLHG 784
           CLIDF   V++D+EY + +G  S  K+L G  +N     GK    KR+L + Q EG+ + 
Sbjct: 724 CLIDFGKPVEEDEEYDLPNGNLS--KTLEGKLVNLGLKKGKGTNRKRRLDEYQLEGKSNE 783

Query: 785 RKNFEIFPFRDPVLLIGHLSKTSLLIIEKPWLEVANTFDAPPVHRHIYGT 821
           RKNFEI P   PVL +GHLSK S+L+IEKPW++V  + D  PV RHI+GT
Sbjct: 784 RKNFEILPSNHPVLFVGHLSKNSILVIEKPWMDVVKSLDNQPVDRHIFGT 815

BLAST of Spg016355 vs. ExPASy Swiss-Prot
Match: Q8R2N2 (U3 small nucleolar RNA-associated protein 4 homolog OS=Mus musculus OX=10090 GN=Utp4 PE=2 SV=3)

HSP 1 Score: 209.1 bits (531), Expect = 1.8e-52
Identity = 196/821 (23.87%), Postives = 350/821 (42.63%), Query Frame = 0

Query: 5   YRNSSIQWKPSPVVALASSADDSQVAAAREDGSLEIWLVSPGSVGWHCQLTISGDPTSRV 64
           +R     + PS +  +A +   +++A +R DG++EI+ +   S  +  +    G  +   
Sbjct: 7   HRVRFFNYVPSGIRCVAYNNQSNRLAVSRTDGTVEIYNL---SANYFQEKFFPGHESRGT 66

Query: 65  SSLVWCCAGSRDRPSGRLLSSSIDGSISEWDLFDLTQKTVLESIGVSIWQIAAAPCNIPE 124
            +L W  AG +     RL S+ ++G I E+DL  L  K  L++ G  IW + A+P     
Sbjct: 67  EALCW--AGGQ-----RLFSAGLNGEILEYDLQALNIKYTLDAFGGPIWSMTASPS---- 126

Query: 125 IHREVKPQDIENGHLTDSESDCQDCSESEDDSNSSEFRVQSSDVNPSLAIACDDGCVRIY 184
                                                          L + C+DG V+++
Sbjct: 127 --------------------------------------------GSQLLVGCEDGSVKLF 186

Query: 185 NICDAEEFVYKRSLPRVSGKVLSVTWSNDAERIFSGSSDGFIRCWNASLGHEIYRITAGL 244
            +   E+  + R+  R   ++LS+ W      + +GS D +I  ++   G  I ++    
Sbjct: 187 EV-TPEKIQFARNFDRQKSRILSLCWHPAGTHVAAGSLD-YISVFDVKSGSIIRKMVLDR 246

Query: 245 GGLGSGPELC-VWSLLSLRCGTLVSADSSGSVQFWDSNHGTLLQAHTLHKGDVNALAVTP 304
             LG     C VW +  L  GT++S DS G VQ WDS  GTL+++H +   DV ++AV  
Sbjct: 247 QHLGVTKSRCIVWGVAFLSDGTVISVDSVGKVQLWDSATGTLVKSHLVANADVQSIAVAD 306

Query: 305 NHNRVYSAGSDGQVILYKLSNENVASSEDQNSSEVMKKWIYVGHVRAHTHDIRALTVAVP 364
             +      ++G V  ++L       S   NSSE  K+W+     + HTHD+RA+     
Sbjct: 307 QEDSFVVGTAEGTVFHFQL------VSMTSNSSE--KQWVRTKPFQHHTHDVRAV----- 366

Query: 365 ICREAELSQDEEVKRVRYKKKPNDFSYRKWAHLGVPMLISGGDDTKLFAYSAQEFTKFSP 424
                                         AH     LISGG DT L      E  +   
Sbjct: 367 ------------------------------AH-SPTALISGGTDTHLVIRPLMERVEVKN 426

Query: 425 HDICPAPQRVPMQLVLNTVFNQAPLLLVQAAHSLDILCVRPKSGSFRDMACGPSKGLAK- 484
           +D        P + ++ +   +  LLL Q AH L++  +   S + ++   G +  L+K 
Sbjct: 427 YDAALRKITFPHRRLI-SCSKRRQLLLFQFAHHLELWRLGSTSATGKN---GDTLPLSKN 486

Query: 485 ADLLVRVKSKASRKIICSTISNSGKLFAYSDHTKPNLFELKKSGGSKCSWTVNRRQLPNT 544
           AD L+ +K+K    IICS +S  G   AYS  ++  L+ LK    +     V++  LP+ 
Sbjct: 487 ADHLLHLKTKGPENIICSCVSPCGSWIAYSTASRFFLYRLKYERDNISLQRVSK--LPSF 546

Query: 545 LPFAHSMVFSFDSSRLIIAGHDKRIYVVDV--GSLEVIHSFTPFRELQDDTLPPAEPPIT 604
           L  A  ++FS DS++L++A +   +++V +  GS + +H+F P    Q  T+      + 
Sbjct: 547 LRSALHILFSEDSTKLLVASNQGSLHIVHLSEGSFKHLHTFQP----QSGTV----EAMC 606

Query: 605 KIFTSSDGQWLAAVNCFGDIYIFNMEIMRQHWFISRLDGASVTAGGFPQWNNNVLVVTTS 664
            +  S DG WLAA      ++++++  ++ H  +   +         P  NN  LV+  S
Sbjct: 607 LLAVSPDGNWLAASGTSAGVHVYDLHHLKLHCTVPAYNFPVTALAIAPNTNN--LVIAHS 666

Query: 665 SNQVYAFDVEAKQLGDWSMR-HTQALPKRFQEFPGEVIGLSFPPSATSLPVIVYSSRAMC 724
             QV+ F +  KQ  +WS     Q   + + +    +  +SF P    + ++++ +   C
Sbjct: 667 DQQVFEFSIPEKQYTEWSRSLQKQGFHQLWLQRDTPITHISFHPK-RPMHILLHDAYMFC 686

Query: 725 LIDFRMSVDQDDEYVMISGQDSTVKSLRGTPINGKLKRKLRDCQTEGRLHGRKNFEIFPF 784
           +ID  + +  +             K++   P+  K +    D       HG K  +I+  
Sbjct: 727 IIDKSLPLPNE-------------KTVLYNPLPPKNE---SDVFLRRTTHGFKMSKIY-- 686

Query: 785 RDPVLLIGHLSKTSLLIIEKPWLEVANTFDAPPVHRHIYGT 821
             P+L +  L + +L+ +E+P  ++      PP+ +  +GT
Sbjct: 787 -KPLLFMDLLDERTLVAVERPLDDIIAQL-PPPIKKKKFGT 686

BLAST of Spg016355 vs. ExPASy Swiss-Prot
Match: Q969X6 (U3 small nucleolar RNA-associated protein 4 homolog OS=Homo sapiens OX=9606 GN=UTP4 PE=1 SV=1)

HSP 1 Score: 204.9 bits (520), Expect = 3.5e-51
Identity = 189/821 (23.02%), Postives = 342/821 (41.66%), Query Frame = 0

Query: 5   YRNSSIQWKPSPVVALASSADDSQVAAAREDGSLEIWLVSPGSVGWHCQLTISGDPTSRV 64
           +R     + PS +  +A +   +++A +R DG++EI+ +   S  +  +    G  +   
Sbjct: 7   HRVRFFNYVPSGIRCVAYNNQSNRLAVSRTDGTVEIYNL---SANYFQEKFFPGHESRAT 66

Query: 65  SSLVWCCAGSRDRPSGRLLSSSIDGSISEWDLFDLTQKTVLESIGVSIWQIAAAPCNIPE 124
            +L W           RL S+ ++G I E+DL  L  K  +++ G  IW +AA+P     
Sbjct: 67  EALCWA-------EGQRLFSAGLNGEIMEYDLQALNIKYAMDAFGGPIWSMAASPS---- 126

Query: 125 IHREVKPQDIENGHLTDSESDCQDCSESEDDSNSSEFRVQSSDVNPSLAIACDDGCVRIY 184
                                                          L + C+DG V+++
Sbjct: 127 --------------------------------------------GSQLLVGCEDGSVKLF 186

Query: 185 NICDAEEFVYKRSLPRVSGKVLSVTWSNDAERIFSGSSDGFIRCWNASLGHEIYRITAGL 244
            I   ++  ++R+  R   ++LS++W      I +GS D +I  ++   G  ++++    
Sbjct: 187 QI-TPDKIQFERNFDRQKSRILSLSWHPSGTHIAAGSID-YISVFDVKSGSAVHKMIVDR 246

Query: 245 GGLGSGPELC-VWSLLSLRCGTLVSADSSGSVQFWDSNHGTLLQAHTLHKGDVNALAVTP 304
             +G     C VW +  L  GT++S DS+G VQFWDS  GTL+++H +   DV ++AV  
Sbjct: 247 QYMGVSKRKCIVWGVAFLSDGTIISVDSAGKVQFWDSATGTLVKSHLIANADVQSIAVAD 306

Query: 305 NHNRVYSAGSDGQVILYKLSNENVASSEDQNSSEVMKKWIYVGHVRAHTHDIRALTVAVP 364
             +      ++G V  ++L      SSE        K+W+     + HTHD+R +     
Sbjct: 307 QEDSFVVGTAEGTVFHFQLVPVTSNSSE--------KQWVRTKPFQHHTHDVRTV----- 366

Query: 365 ICREAELSQDEEVKRVRYKKKPNDFSYRKWAHLGVPMLISGGDDTKLFAYSAQEFTKFSP 424
                                         AH     LISGG DT L      E  +   
Sbjct: 367 ------------------------------AH-SPTALISGGTDTHLVFRPLMEKVEVKN 426

Query: 425 HDICPAPQRVPMQLVLNTVFNQAPLLLVQAAHSLDILCVRPKSGSFRDMACGPSKGLAK- 484
           +D        P + ++ +   +  LLL Q AH L++  +     + ++   G +  L+K 
Sbjct: 427 YDAALRKITFPHRCLI-SCSKKRQLLLFQFAHHLELWRLGSTVATGKN---GDTLPLSKN 486

Query: 485 ADLLVRVKSKASRKIICSTISNSGKLFAYSDHTKPNLFELKKSGGSKCSWTVNRRQLPNT 544
           AD L+ +K+K    IICS IS  G   AYS  ++  L+ L     +     V++  +P  
Sbjct: 487 ADHLLHLKTKGPENIICSCISPCGSWIAYSTVSRFFLYRLNYEHDNISLKRVSK--MPAF 546

Query: 545 LPFAHSMVFSFDSSRLIIAGHDKRIYVVDV--GSLEVIHSFTPFRELQDDTLPPAEPPIT 604
           L  A  ++FS DS++L +A +   +++V +  GS + +H+F P    Q  T+      + 
Sbjct: 547 LRSALQILFSEDSTKLFVASNQGALHIVQLSGGSFKHLHAFQP----QSGTV----EAMC 606

Query: 605 KIFTSSDGQWLAAVNCFGDIYIFNMEIMRQHWFISRLDGASVTAGGFPQWNNNVLVVTTS 664
            +  S DG WLAA      ++++N++ ++ H  +   +         P  NN  LV+  S
Sbjct: 607 LLAVSPDGNWLAASGTSAGVHVYNVKQLKLHCTVPAYNFPVTAMAIAPNTNN--LVIAHS 666

Query: 665 SNQVYAFDVEAKQLGDWSMR-HTQALPKRFQEFPGEVIGLSFPPSATSLPVIVYSSRAMC 724
             QV+ + +  KQ  DWS     Q     + +    +  +SF P    + ++++ +   C
Sbjct: 667 DQQVFEYSIPDKQYTDWSRTVQKQGFHHLWLQRDTPITHISFHPK-RPMHILLHDAYMFC 686

Query: 725 LIDFRMSVDQDDEYVMISGQDSTVKSLRGTPINGKLKRKLRDCQTEGRLHGRKNFEIFPF 784
           +ID  + +  D             K+L   P     +  +       R      F+I   
Sbjct: 727 IIDKSLPLPND-------------KTLLYNPFPPTNESDVI------RRRTAHAFKISKI 686

Query: 785 RDPVLLIGHLSKTSLLIIEKPWLEVANTFDAPPVHRHIYGT 821
             P+L +  L + +L+ +E+P  ++      PP+ +  +GT
Sbjct: 787 YKPLLFMDLLDERTLVAVERPLDDIIAQL-PPPIKKKKFGT 686

BLAST of Spg016355 vs. ExPASy Swiss-Prot
Match: O60161 (U3 small nucleolar RNA-associated protein 4 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=utp4 PE=3 SV=1)

HSP 1 Score: 187.2 bits (474), Expect = 7.5e-46
Identity = 196/844 (23.22%), Postives = 342/844 (40.52%), Query Frame = 0

Query: 2   IEFYRNSSIQWKPSPVVALASSADDSQ---------VAAAREDGSLEIWLVSPGSVGWHC 61
           ++ +R   I + PS + A+A S    Q         +A  R  G++EIW  +P +  W  
Sbjct: 1   MDIHRCRFIDYTPSAITAMAFSHKSGQNDSMPNNLLLAVGRASGNIEIW--NPRN-DWCL 60

Query: 62  QLTISGDPTSRVSSLVWCCAGSRDRPSGRLLSSSIDGSISEWDLFDLTQKTVLESIGVSI 121
           +  + G     +  +VW    S      RL S     +I+EW+L         +S   +I
Sbjct: 61  KTVLYGGVDRSIEGIVW----STGEEELRLFSIGFSTTITEWNLHTGKPLVNQDSNAGAI 120

Query: 122 WQIAAAPCNIPEIHREVKPQDIENGHLTDSESDCQDCSESEDDSNSSEFRVQSSDVNPSL 181
           W IA                                C E++                 +L
Sbjct: 121 WSIAI-------------------------------CDETK-----------------TL 180

Query: 182 AIACDDGCVRIYNICDAEEFV-YKRSLPRVSGKVLSVTWSNDAERIFSGSSDGFIRCWNA 241
           A+ CDDG   +++I      + +KR L R + ++LS+ +    + +  G +DG I+ W+ 
Sbjct: 181 AVGCDDGSCVLFDISGGPGVIEFKRVLMRQTSRILSLDFQT-KDHLVGGCADGVIKVWDL 240

Query: 242 SLGHEIYRITAGLGGLGSGPELCVWSLLSLRCGTLVSADSSGSVQFWDSNHGTLLQAHTL 301
           S  +        +     G    +W++ SLR GT+VSADSSG+V+FW+    TL Q+  L
Sbjct: 241 STPNSAIISRMQVDRARKGEAALIWAVKSLRDGTIVSADSSGAVKFWNGKFFTLSQSFKL 300

Query: 302 HKGDVNALAVTPNHNRVYSAGSDGQVILYKLSNENVASSEDQNSSEVMKKWIYVGHVRAH 361
           H  D   L V+ N + V+S+G D + I Y                   ++W+     R H
Sbjct: 301 HLADALCLGVSANGDMVFSSGIDRKTIQYSREGGK-------------REWVSNSFRRFH 360

Query: 362 THDIRALTVAVPICREAELSQDEEVKRVRYKKKPNDFSYRKWAHLGVPMLISGGDDTKLF 421
           +HD+R +  AV  C+  +                              +LISGG D  L 
Sbjct: 361 SHDVRCM--AVFECKSLD------------------------------VLISGGMDMMLA 420

Query: 422 AYSAQEFTKFSPHDICPAPQRVPMQLVLNTVFNQAPLLLVQAAHSLDILCVRPKSGSFRD 481
               ++F + +   I   PQR  M      V  +A L ++   H  ++L  R  S  +R 
Sbjct: 421 VIPVRQFNRKNHRMISAVPQRPRM-----AVAPKARLFMLWNDH--EVLLWRIGSPGYR- 480

Query: 482 MACGPSKGLAKADLLVRVKSKASRKIICSTISNSGKLFAYSDHTKPNLFELKKSGGSKCS 541
                         L+++       I  + IS  G+L A S   +  L++L+ S  +   
Sbjct: 481 -------------FLLKIVLADEENISHAAISPDGELIAISSVLRTKLYQLQYSDENVKV 540

Query: 542 WTVNRRQLPNTLPFAHSMVFSFDSSRLIIAGHDKRIYVVDVG-----SLEVIHSFTP--- 601
            TV    L N    A  + F+ D ++LI+  +D  I+++++       LEV     P   
Sbjct: 541 ETVEDSFLSNI--GASLLSFTVDKNKLILVSNDSEIFLIELSRLDSRQLEVFELSQPTSK 600

Query: 602 -FRELQDDTLPPAEPPITKIFTSSDGQWLAAVNCFGDIYIFNMEIMRQHWFISRLDGASV 661
                Q   +      I  I  SSDG + A  +  G+I+ +++  +  +  + R++   V
Sbjct: 601 KIAPRQRSNVSSMCDGICSIAVSSDGDYFAVADTVGNIFCYSLSNL-TYSELPRVN-TYV 660

Query: 662 TAGGFPQWNNNVLVVTTSSNQVYAFDVEAKQLGDWSMRHTQALPKRFQEFPGEVIGLSFP 721
            A  F       L V T+ NQVY FDV++++L +WS  ++  +PK F +   +  G  F 
Sbjct: 661 RAMAFRPDVRGRLAVATAGNQVYEFDVQSRKLSEWSKNNSTNMPKEFSQLLDKAFGAFFD 710

Query: 722 PSATSLPVIVYSSRAMCLIDFRMSVDQDDEYVMISGQDSTVKSLRGTPINGKLK-RKLRD 781
               S    ++S+  +   D  + +         +G+    K      ++G L  +KL +
Sbjct: 721 SKHPS-RFWIWSANWVSFFDLNLQLPAP----RAAGKR---KIEMNATVDGNLNDKKLAN 710

Query: 782 CQTEGRLH----GRKNFEIFPFRDPVLLIGHLSKTSLLIIEKPWLEVANTFDAP-PVHRH 821
             + G  +      + F I     P+LL+G +  + LL++E+P  ++  +   P   + H
Sbjct: 781 ANSNGISNYGTGDSRCFWITHKYRPMLLVGSVGNSELLVVERPIADMLMSKSMPASFYEH 710

BLAST of Spg016355 vs. ExPASy Swiss-Prot
Match: Q06679 (U3 small nucleolar RNA-associated protein 4 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=UTP4 PE=1 SV=1)

HSP 1 Score: 109.0 bits (271), Expect = 2.6e-22
Identity = 155/734 (21.12%), Postives = 276/734 (37.60%), Query Frame = 0

Query: 5   YRNSSIQWKPSPVVALASS---------ADDSQVAAAREDGSLEIWLVSPGSVGWHCQLT 64
           +R   + + P+ + +LA S           D ++A  R +G++EIW  +P +  W  ++ 
Sbjct: 34  HRCRFVDFTPATITSLAFSHKSNINKLTPSDLRLAIGRSNGNIEIW--NPRN-NWFQEMV 93

Query: 65  ISGDPTSRVSSLVWCCAGSRDRPSGRLLSSSIDGSISEWDLFDLTQKTVLESIGVSIWQI 124
           I G     +  L W    + +  S RL S      ++EWDL         +     IW I
Sbjct: 94  IEGGKDRSIEGLCW---SNVNGESLRLFSIGGSTVVTEWDLATGLPLRNYDCNSGVIWSI 153

Query: 125 AAAPCNIPEIHREVKPQDIENGHLTDSESDCQDCSESEDDSNSSEFRVQSSDVNPSLAIA 184
                                     S +D QD                       L++ 
Sbjct: 154 --------------------------SINDSQD----------------------KLSVG 213

Query: 185 CDDGCVRIYNICDAEEFV-YKRSLPRVSGKVLSVTWSNDAERIFSGSSDGFIRCWNA--- 244
           CD+G V + +I      + +   L R   +VL++ W  D + +  G SDG IR W+A   
Sbjct: 214 CDNGTVVLIDISGGPGVLEHDTILMRQEARVLTLAWKKD-DFVIGGCSDGRIRIWSAQKN 273

Query: 245 --SLGHEIYRITAGLGGLGSGPELCVWSLLSL-RCGTLVSADSSGSVQFWDSNHGTLLQA 304
             ++G  ++ +        S     VWS++ L R   + S DS+GS++FWD    TL Q+
Sbjct: 274 DENMGRLLHTMKVDKAKKES---TLVWSVIYLPRTDQIASGDSTGSIKFWDFQFATLNQS 333

Query: 305 HTLHKGDVNALAVTPNHNRVYSAGSDGQVILYKLSNENVASSEDQNSSEVMKKWIYVGHV 364
              H  DV  L    ++N V+SAG D ++  +         S++ N S+   +W+   + 
Sbjct: 334 FKAHDADVLCLTTDTDNNYVFSAGVDRKIFQF---------SQNTNKSQKNNRWVNSSNR 393

Query: 365 RAHTHDIRALTVAVPICREAELSQDEEVKRVRYKKKPNDFSYRKWAHLGVPMLISGGDDT 424
             H +DIRA      IC               Y+ K  DF            L+SGG + 
Sbjct: 394 LLHGNDIRA------IC--------------AYQSKGADF------------LVSGGVEK 453

Query: 425 KLFAYSAQEFTKFSPHDICPAPQRVPMQLVLNTVFNQAPLLLVQAAHSLDILCVRPKSGS 484
            L   S    T FS  +    P   P     N + N+   L+V  + S   +       S
Sbjct: 454 TLVINS---LTSFSNGNYRKMPTVEPYS--KNVLVNKEQRLVVSWSESTVKIWTMGTDSS 513

Query: 485 FRDMACGPSKGLAKADLLVRVKSKASRKIICSTISNSGKLFAYSDHTKPNLFELKKSGGS 544
                           L+ ++  K  + I   ++S  G++      +   +F L+  G  
Sbjct: 514 TEQ----------NYKLVCKLTLKDDQNISTCSLSPDGQVLVVGRPSTTKVFHLQPVGNK 573

Query: 545 KCSWTVNRRQLPNTLPFAHSMVFSFDSSRLIIAGHDKRIYVVDVGSLEVIHSFTPFRELQ 604
                ++   L  T   +  +V   D+S+++I   +  +++VD+ S              
Sbjct: 574 LKVTKLDNDLLLRT---STKLVKFIDNSKIVICSCEDDVFIVDLES-------------- 633

Query: 605 DDTLPPAEPPITKIFTSSDGQWLAAVNCFGDIYIFN--MEIMRQHWFISRLDGASVTAGG 664
           ++   P E  + ++ ++     +  +N    + +      I R    +  LD  +  +  
Sbjct: 634 EEDEKPQEVELLEVTSTKSSIKVPYINRINHLEVDQNIAVISRGCGVVDILDLKARISKP 636

Query: 665 FPQWNNNV-----------LVVTTSSNQVYAFDV----EAKQ------LGDWSMRHTQAL 700
             + NN +           +VV T+ N++Y F++    EA+       L  WS  +T  L
Sbjct: 694 LARLNNFITAVHINTSRKSVVVITADNKIYEFNMNLNSEAENEDSESVLTQWSKNNTDNL 636

BLAST of Spg016355 vs. ExPASy TrEMBL
Match: A0A6J1F7H5 (U3 small nucleolar RNA-associated protein 4 homolog isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111442803 PE=4 SV=1)

HSP 1 Score: 1557.7 bits (4032), Expect = 0.0e+00
Identity = 752/820 (91.71%), Postives = 786/820 (95.85%), Query Frame = 0

Query: 1   MIEFYRNSSIQWKPSPVVALASSADDSQVAAAREDGSLEIWLVSPGSVGWHCQLTISGDP 60
           MIEFYRNSSI+WKPSPVVALASSAD SQVAAAREDGSLEIWLVSPGSVGWHCQLTI+GDP
Sbjct: 1   MIEFYRNSSIEWKPSPVVALASSADGSQVAAAREDGSLEIWLVSPGSVGWHCQLTINGDP 60

Query: 61  TSRVSSLVWCCAGSRDRPSGRLLSSSIDGSISEWDLFDLTQKTVLESIGVSIWQIAAAPC 120
           TSRVSSLVWC AGS+DRPSGRL SSSIDGSISEWDLF LTQK+VLESIG+SIWQIAAAPC
Sbjct: 61  TSRVSSLVWCRAGSKDRPSGRLFSSSIDGSISEWDLFGLTQKSVLESIGLSIWQIAAAPC 120

Query: 121 NIPEIHREVKPQDIENGHLTDSESDCQDCSESEDDSNSSEFRVQSSDVNPSLAIACDDGC 180
           N  E+HREVKP++IEN H+TD+ESDCQDCSESEDDSNSS+  VQ+SD +P LAIACDDGC
Sbjct: 121 NSREVHREVKPKEIENAHVTDNESDCQDCSESEDDSNSSKLHVQASDEDPCLAIACDDGC 180

Query: 181 VRIYNICDAEEFVYKRSLPRVSGKVLSVTWSNDAERIFSGSSDGFIRCWNASLGHEIYRI 240
           VRIYNICD EE +YKRSLPRVSG+VLSVTWSNDAERIFSGSSDGFIRCWNASLGHE+YRI
Sbjct: 181 VRIYNICDVEELIYKRSLPRVSGRVLSVTWSNDAERIFSGSSDGFIRCWNASLGHEVYRI 240

Query: 241 TAGLGGLGSGPELCVWSLLSLRCGTLVSADSSGSVQFWDSNHGTLLQAHTLHKGDVNALA 300
           TAGLGGLGSG ELCVWSLL LRCGTLVSADS+GSVQFWDSNHGTLLQAHTLHKGDVNALA
Sbjct: 241 TAGLGGLGSGSELCVWSLLFLRCGTLVSADSTGSVQFWDSNHGTLLQAHTLHKGDVNALA 300

Query: 301 VTPNHNRVYSAGSDGQVILYKLSNENVASSEDQNSSEVMKKWIYVGHVRAHTHDIRALTV 360
            TPNHNRVYSAGSDGQVILYKLSNENV SSED+ SSE+MKKWIYVGHVRAHTHDIRALTV
Sbjct: 301 ATPNHNRVYSAGSDGQVILYKLSNENVLSSEDKYSSEMMKKWIYVGHVRAHTHDIRALTV 360

Query: 361 AVPICREAELSQDEEVKRVRYKKKPNDFSYRKWAHLGVPMLISGGDDTKLFAYSAQEFTK 420
           AVPICREAE SQD EVKR+R+K+KPNDFSY KWAHLGVPML+SGGDDTKLFAYSAQEFTK
Sbjct: 361 AVPICREAEASQDVEVKRIRHKRKPNDFSYHKWAHLGVPMLMSGGDDTKLFAYSAQEFTK 420

Query: 421 FSPHDICPAPQRVPMQLVLNTVFNQAPLLLVQAAHSLDILCVRPKSGSFRDMACGPSKGL 480
           FSPHDICPAPQRVPMQLVLNTVFNQAPLLLVQAAHSLDILCVRPK GSF D  CGPSKGL
Sbjct: 421 FSPHDICPAPQRVPMQLVLNTVFNQAPLLLVQAAHSLDILCVRPKCGSFGDKTCGPSKGL 480

Query: 481 AKADLLVRVKSKASRKIICSTISNSGKLFAYSDHTKPNLFELKKSGGSKCSWTVNRRQLP 540
           AKADLLVRVKSKASRKIICSTISNSGKLFA+SDH KPNL+ELKKSGGSKCSWTVNRR+LP
Sbjct: 481 AKADLLVRVKSKASRKIICSTISNSGKLFAFSDHAKPNLYELKKSGGSKCSWTVNRRKLP 540

Query: 541 NTLPFAHSMVFSFDSSRLIIAGHDKRIYVVDVGSLEVIHSFTPFRELQDDTLPPAEPPIT 600
           NTLPFAHSMVFSFDSSRLIIAGHDKRIYVVDV SLEV+HSFTPFRE QDD LPPAEPPIT
Sbjct: 541 NTLPFAHSMVFSFDSSRLIIAGHDKRIYVVDVSSLEVLHSFTPFREQQDDILPPAEPPIT 600

Query: 601 KIFTSSDGQWLAAVNCFGDIYIFNMEIMRQHWFISRLDGASVTAGGFPQWNNNVLVVTTS 660
           KIFTSSDGQWLAAVNCFGDIY+FNMEI+RQHWFISRLDGAS+TAGGFPQWNNNVLVVTTS
Sbjct: 601 KIFTSSDGQWLAAVNCFGDIYVFNMEILRQHWFISRLDGASITAGGFPQWNNNVLVVTTS 660

Query: 661 SNQVYAFDVEAKQLGDWSMRHTQALPKRFQEFPGEVIGLSFPPSATSLPVIVYSSRAMCL 720
           SNQ+YAFD+EAKQLGDWSMRHTQALPKRFQEFPGEVIGLSFPPS TSLPVIVYSSRAMCL
Sbjct: 661 SNQIYAFDIEAKQLGDWSMRHTQALPKRFQEFPGEVIGLSFPPSTTSLPVIVYSSRAMCL 720

Query: 721 IDFRMSVDQDDEYVMISGQDSTVKSLRGTPINGKLKRKLRDCQTEGRLHGRKNFEIFPFR 780
           IDFRMSVDQ+DEYVMISGQDSTVK+L GTPINGKLKRKLRDCQTEGR HGRKNFE FPFR
Sbjct: 721 IDFRMSVDQNDEYVMISGQDSTVKNLWGTPINGKLKRKLRDCQTEGRPHGRKNFENFPFR 780

Query: 781 DPVLLIGHLSKTSLLIIEKPWLEVANTFDAPPVHRHIYGT 821
           DPVLLIGHLSKTSLLIIEKPWLEV NTFDAPPVHRHIYGT
Sbjct: 781 DPVLLIGHLSKTSLLIIEKPWLEVVNTFDAPPVHRHIYGT 820

BLAST of Spg016355 vs. ExPASy TrEMBL
Match: A0A6J1F6K3 (U3 small nucleolar RNA-associated protein 4 homolog isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111442803 PE=4 SV=1)

HSP 1 Score: 1553.1 bits (4020), Expect = 0.0e+00
Identity = 752/821 (91.60%), Postives = 786/821 (95.74%), Query Frame = 0

Query: 1   MIEFYRNSSIQWKPSPVVALASSADDSQVAAAREDGSLEIWLVSPGSVGWHCQLTISGDP 60
           MIEFYRNSSI+WKPSPVVALASSAD SQVAAAREDGSLEIWLVSPGSVGWHCQLTI+GDP
Sbjct: 1   MIEFYRNSSIEWKPSPVVALASSADGSQVAAAREDGSLEIWLVSPGSVGWHCQLTINGDP 60

Query: 61  TSRVSSLVWCCAGSRDRPSGRLLSSSIDGSISEWDLFDLTQKTVLESIGVSIWQIAAAPC 120
           TSRVSSLVWC AGS+DRPSGRL SSSIDGSISEWDLF LTQK+VLESIG+SIWQIAAAPC
Sbjct: 61  TSRVSSLVWCRAGSKDRPSGRLFSSSIDGSISEWDLFGLTQKSVLESIGLSIWQIAAAPC 120

Query: 121 NIPEIHREVKPQDIENGHLTDSESDCQDCSESEDDSNSSEFRVQSSDVNPSLAIACDDGC 180
           N  E+HREVKP++IEN H+TD+ESDCQDCSESEDDSNSS+  VQ+SD +P LAIACDDGC
Sbjct: 121 NSREVHREVKPKEIENAHVTDNESDCQDCSESEDDSNSSKLHVQASDEDPCLAIACDDGC 180

Query: 181 VRIYNICDAEEFVYKRSLPRVSGKVLSVTWSNDAERIFSGSSDGFIRCWNASLGHEIYRI 240
           VRIYNICD EE +YKRSLPRVSG+VLSVTWSNDAERIFSGSSDGFIRCWNASLGHE+YRI
Sbjct: 181 VRIYNICDVEELIYKRSLPRVSGRVLSVTWSNDAERIFSGSSDGFIRCWNASLGHEVYRI 240

Query: 241 TAGLGGLGSGPELCVWSLLSLRCGTLVSADSSGSVQFWDSNHGTLLQAHTLHKGDVNALA 300
           TAGLGGLGSG ELCVWSLL LRCGTLVSADS+GSVQFWDSNHGTLLQAHTLHKGDVNALA
Sbjct: 241 TAGLGGLGSGSELCVWSLLFLRCGTLVSADSTGSVQFWDSNHGTLLQAHTLHKGDVNALA 300

Query: 301 VTPNHNRVYSAGSDGQVILYKLSNENVASSEDQNSSEVMKKWIYVGHVRAHTHDIRALTV 360
            TPNHNRVYSAGSDGQVILYKLSNENV SSED+ SSE+MKKWIYVGHVRAHTHDIRALTV
Sbjct: 301 ATPNHNRVYSAGSDGQVILYKLSNENVLSSEDKYSSEMMKKWIYVGHVRAHTHDIRALTV 360

Query: 361 AVPICRE-AELSQDEEVKRVRYKKKPNDFSYRKWAHLGVPMLISGGDDTKLFAYSAQEFT 420
           AVPICRE AE SQD EVKR+R+K+KPNDFSY KWAHLGVPML+SGGDDTKLFAYSAQEFT
Sbjct: 361 AVPICREAAEASQDVEVKRIRHKRKPNDFSYHKWAHLGVPMLMSGGDDTKLFAYSAQEFT 420

Query: 421 KFSPHDICPAPQRVPMQLVLNTVFNQAPLLLVQAAHSLDILCVRPKSGSFRDMACGPSKG 480
           KFSPHDICPAPQRVPMQLVLNTVFNQAPLLLVQAAHSLDILCVRPK GSF D  CGPSKG
Sbjct: 421 KFSPHDICPAPQRVPMQLVLNTVFNQAPLLLVQAAHSLDILCVRPKCGSFGDKTCGPSKG 480

Query: 481 LAKADLLVRVKSKASRKIICSTISNSGKLFAYSDHTKPNLFELKKSGGSKCSWTVNRRQL 540
           LAKADLLVRVKSKASRKIICSTISNSGKLFA+SDH KPNL+ELKKSGGSKCSWTVNRR+L
Sbjct: 481 LAKADLLVRVKSKASRKIICSTISNSGKLFAFSDHAKPNLYELKKSGGSKCSWTVNRRKL 540

Query: 541 PNTLPFAHSMVFSFDSSRLIIAGHDKRIYVVDVGSLEVIHSFTPFRELQDDTLPPAEPPI 600
           PNTLPFAHSMVFSFDSSRLIIAGHDKRIYVVDV SLEV+HSFTPFRE QDD LPPAEPPI
Sbjct: 541 PNTLPFAHSMVFSFDSSRLIIAGHDKRIYVVDVSSLEVLHSFTPFREQQDDILPPAEPPI 600

Query: 601 TKIFTSSDGQWLAAVNCFGDIYIFNMEIMRQHWFISRLDGASVTAGGFPQWNNNVLVVTT 660
           TKIFTSSDGQWLAAVNCFGDIY+FNMEI+RQHWFISRLDGAS+TAGGFPQWNNNVLVVTT
Sbjct: 601 TKIFTSSDGQWLAAVNCFGDIYVFNMEILRQHWFISRLDGASITAGGFPQWNNNVLVVTT 660

Query: 661 SSNQVYAFDVEAKQLGDWSMRHTQALPKRFQEFPGEVIGLSFPPSATSLPVIVYSSRAMC 720
           SSNQ+YAFD+EAKQLGDWSMRHTQALPKRFQEFPGEVIGLSFPPS TSLPVIVYSSRAMC
Sbjct: 661 SSNQIYAFDIEAKQLGDWSMRHTQALPKRFQEFPGEVIGLSFPPSTTSLPVIVYSSRAMC 720

Query: 721 LIDFRMSVDQDDEYVMISGQDSTVKSLRGTPINGKLKRKLRDCQTEGRLHGRKNFEIFPF 780
           LIDFRMSVDQ+DEYVMISGQDSTVK+L GTPINGKLKRKLRDCQTEGR HGRKNFE FPF
Sbjct: 721 LIDFRMSVDQNDEYVMISGQDSTVKNLWGTPINGKLKRKLRDCQTEGRPHGRKNFENFPF 780

Query: 781 RDPVLLIGHLSKTSLLIIEKPWLEVANTFDAPPVHRHIYGT 821
           RDPVLLIGHLSKTSLLIIEKPWLEV NTFDAPPVHRHIYGT
Sbjct: 781 RDPVLLIGHLSKTSLLIIEKPWLEVVNTFDAPPVHRHIYGT 821

BLAST of Spg016355 vs. ExPASy TrEMBL
Match: A0A6J1C0W2 (U3 small nucleolar RNA-associated protein 4 homolog isoform X1 OS=Momordica charantia OX=3673 GN=LOC111007425 PE=4 SV=1)

HSP 1 Score: 1552.7 bits (4019), Expect = 0.0e+00
Identity = 761/821 (92.69%), Postives = 787/821 (95.86%), Query Frame = 0

Query: 1   MIEFYRNSSIQWKPSPVVALASSADDSQVAAAREDGSLEIWLVSPGSVGWHCQLTISGDP 60
           MIEFYRNSSI WKPSPVVALASSADDSQ+AAAREDGSLEIWLVSPGSVGWHCQL ISGD 
Sbjct: 1   MIEFYRNSSIDWKPSPVVALASSADDSQIAAAREDGSLEIWLVSPGSVGWHCQLMISGDT 60

Query: 61  TSRVSSLVWCCAGSRDRPSGRLLSSSIDGSISEWDLFDLTQKTVLESIGVSIWQIAAAPC 120
             RVSSLVWCCAG +D PSGRL SSSIDGSISEWDLFDL QKTVLESIGVSIWQIAAAPC
Sbjct: 61  NYRVSSLVWCCAGLKDSPSGRLFSSSIDGSISEWDLFDLKQKTVLESIGVSIWQIAAAPC 120

Query: 121 NIPEIHREVKPQDIENGHLTDSESDCQDCSESEDDSNSSEFRVQSSDVNPSLAIACDDGC 180
           N   + R+VK QD++NGHL D+E+  QDCSES+DDSNSSE  VQS D NPSLA+ACDDGC
Sbjct: 121 NSRGLDRKVKAQDMQNGHLIDNENGYQDCSESDDDSNSSELHVQSRDENPSLAVACDDGC 180

Query: 181 VRIYNICDAEEFVYKRSLPRVSGKVLSVTWSNDAERIFSGSSDGFIRCWNASLGHEIYRI 240
           VR+YNI DAEEF+YKRSLPRVSG+VLSVTWSNDAERIFSGSSDGFIRCWNASLGHEIYRI
Sbjct: 181 VRLYNI-DAEEFIYKRSLPRVSGRVLSVTWSNDAERIFSGSSDGFIRCWNASLGHEIYRI 240

Query: 241 TAGLGGLGSGPELCVWSLLSLRCGTLVSADSSGSVQFWDSNHGTLLQAHTLHKGDVNALA 300
           T GLGGLGSGPELCVWSLLSLR GTLVSADSSGSVQFWDSNHGTLLQAHTLHKGDVNALA
Sbjct: 241 TVGLGGLGSGPELCVWSLLSLRSGTLVSADSSGSVQFWDSNHGTLLQAHTLHKGDVNALA 300

Query: 301 VTPNHNRVYSAGSDGQVILYKLSNENVASSEDQNSSEVMKKWIYVGHVRAHTHDIRALTV 360
             PNHNRVYSAGSDGQVILYKLSNENVASSED+ SSEVMKKWIYVGHVRAHTHDIRALTV
Sbjct: 301 AAPNHNRVYSAGSDGQVILYKLSNENVASSEDKYSSEVMKKWIYVGHVRAHTHDIRALTV 360

Query: 361 AVPICREAELSQDEEVKRVRYKKKPNDFSYRKWAHLGVPMLISGGDDTKLFAYSAQEFTK 420
           AVPICREAELSQD+EVKRVR KKKPNDFSYRKWAHLGVPMLISGGDDTKLFAYSAQEFTK
Sbjct: 361 AVPICREAELSQDDEVKRVRRKKKPNDFSYRKWAHLGVPMLISGGDDTKLFAYSAQEFTK 420

Query: 421 FSPHDICPAPQRVPMQLVLNTVFNQAPLLLVQAAHSLDILCVRPKSGSFRDMACGPSKGL 480
           FSPHDICPAPQRVPMQLVLNT+FNQAPLLLVQAAHSLDILCVRPKSGSFRDMACGPSKG 
Sbjct: 421 FSPHDICPAPQRVPMQLVLNTMFNQAPLLLVQAAHSLDILCVRPKSGSFRDMACGPSKGH 480

Query: 481 AKADLLVRVKSKASRKIICSTISNSGKLFAYSDHTKPNLFELKKSGGSKCSWTVNRRQLP 540
           A+ADLLVRVKSKASRKIICS+ISNSGKLFAYSDHTKPNLFELKKSGGSKCSWTVNRRQLP
Sbjct: 481 AQADLLVRVKSKASRKIICSSISNSGKLFAYSDHTKPNLFELKKSGGSKCSWTVNRRQLP 540

Query: 541 NTLPFAHSMVFSFDSSRLIIAGHDKRIYVVDVGSLEVIHSFTPFRELQDDTLPPAEPPIT 600
           ++LPFAHSMVFSFDSSRLIIAGHDKRIYVVDV SLEVIHSFTPFRELQDDTLPPAEPPIT
Sbjct: 541 HSLPFAHSMVFSFDSSRLIIAGHDKRIYVVDVVSLEVIHSFTPFRELQDDTLPPAEPPIT 600

Query: 601 KIFTSSDGQWLAAVNCFGDIYIFNMEIMRQHWFISRLDGASVTAGGFPQWNNNVLVVTTS 660
           K+FTSSDGQWLAAVNCFGDIYIFNMEIMRQHWFISRLDGAS+TAGGFPQWNNNVLVVTTS
Sbjct: 601 KVFTSSDGQWLAAVNCFGDIYIFNMEIMRQHWFISRLDGASITAGGFPQWNNNVLVVTTS 660

Query: 661 SNQVYAFDVEAKQLGDWSMRHTQALPKRFQEFPGEVIGLSFPPSATSLPVIVYSSRAMCL 720
           SNQVYAFDVEAK+LGDWSMRHTQALPKRFQEFPGEVIGLSFPPSATSLPVIVYSSRAMCL
Sbjct: 661 SNQVYAFDVEAKKLGDWSMRHTQALPKRFQEFPGEVIGLSFPPSATSLPVIVYSSRAMCL 720

Query: 721 IDFRMSVDQDDEYVMISGQDSTVKSLRGTP-INGKLKRKLRDCQTEGRLHGRKNFEIFPF 780
           IDFRMSVDQDDEYVMISGQDS VK+LRGTP INGKLKRKLRDCQTEGRLHGRKNFEIFPF
Sbjct: 721 IDFRMSVDQDDEYVMISGQDSAVKNLRGTPTINGKLKRKLRDCQTEGRLHGRKNFEIFPF 780

Query: 781 RDPVLLIGHLSKTSLLIIEKPWLEVANTFDAPPVHRHIYGT 821
           RDPVLLIGHLSK SLLI++KPWLEVANTFDA PVHRHIYGT
Sbjct: 781 RDPVLLIGHLSKNSLLIMDKPWLEVANTFDAAPVHRHIYGT 820

BLAST of Spg016355 vs. ExPASy TrEMBL
Match: A0A6J1FM21 (U3 small nucleolar RNA-associated protein 4-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111446446 PE=4 SV=1)

HSP 1 Score: 1552.7 bits (4019), Expect = 0.0e+00
Identity = 757/820 (92.32%), Postives = 783/820 (95.49%), Query Frame = 0

Query: 1   MIEFYRNSSIQWKPSPVVALASSADDSQVAAAREDGSLEIWLVSPGSVGWHCQLTISGDP 60
           MIE YRNSSI+WKPSPVVALASSADDSQVAAAREDGSLEIWLVSPGSVGWHCQLTI GDP
Sbjct: 1   MIECYRNSSIEWKPSPVVALASSADDSQVAAAREDGSLEIWLVSPGSVGWHCQLTIIGDP 60

Query: 61  TSRVSSLVWCCAGSRDRPSGRLLSSSIDGSISEWDLFDLTQKTVLESIGVSIWQIAAAPC 120
           TSRVSSLVWCC GS+DRPSGRL SSSIDGSISEWDLFDLTQK VLESIG SIWQIAAAPC
Sbjct: 61  TSRVSSLVWCCTGSKDRPSGRLFSSSIDGSISEWDLFDLTQKVVLESIGFSIWQIAAAPC 120

Query: 121 NIPEIHREVKPQDIENGHLTDSESDCQDCSESEDDSNSSEFRVQSSDVNPSLAIACDDGC 180
           N  E+  EVKPQD++NGHLTDSESD QDCSESEDDSNSSE  VQSSD NPSLAIACDDGC
Sbjct: 121 NSREVDSEVKPQDMQNGHLTDSESDFQDCSESEDDSNSSELHVQSSDENPSLAIACDDGC 180

Query: 181 VRIYNICDAEEFVYKRSLPRVSGKVLSVTWSNDAERIFSGSSDGFIRCWNASLGHEIYRI 240
           VRIYNICDAEEF+YKRSLPRVSG+VLSVTWSNDAERIFSGSSDGFIRCWN SLGHEIYRI
Sbjct: 181 VRIYNICDAEEFIYKRSLPRVSGRVLSVTWSNDAERIFSGSSDGFIRCWNISLGHEIYRI 240

Query: 241 TAGLGGLGSGPELCVWSLLSLRCGTLVSADSSGSVQFWDSNHGTLLQAHTLHKGDVNALA 300
           TAGLGGLGSGPELCVWSLL LRCGTLVSADS+GSVQFW+ NHGTLLQAHTLHKGDVN LA
Sbjct: 241 TAGLGGLGSGPELCVWSLLYLRCGTLVSADSTGSVQFWNGNHGTLLQAHTLHKGDVNKLA 300

Query: 301 VTPNHNRVYSAGSDGQVILYKLSNENVASSEDQNSSEVMKKWIYVGHVRAHTHDIRALTV 360
            TPN NRVYSAGSDGQVILYKLS+ENVAS +D+ SSEVMKKWIYVGHVR+HTHDIRALTV
Sbjct: 301 ATPNQNRVYSAGSDGQVILYKLSSENVASHDDECSSEVMKKWIYVGHVRSHTHDIRALTV 360

Query: 361 AVPICREAELSQDEEVKRVRYKKKPNDFSYRKWAHLGVPMLISGGDDTKLFAYSAQEFTK 420
           AVPICREAEL QD+EVKR R  KKPNDFSYRKWAHLGVPMLISGGDD KLFAYSAQEFTK
Sbjct: 361 AVPICREAELPQDDEVKRAR-NKKPNDFSYRKWAHLGVPMLISGGDDAKLFAYSAQEFTK 420

Query: 421 FSPHDICPAPQRVPMQLVLNTVFNQAPLLLVQAAHSLDILCVRPKSGSFRDMACGPSKGL 480
           FSPHDICPAPQRVPMQLVLNT+FNQAPLLLVQAAHSLDILC+RPKSGSFRDMACGPSKGL
Sbjct: 421 FSPHDICPAPQRVPMQLVLNTIFNQAPLLLVQAAHSLDILCIRPKSGSFRDMACGPSKGL 480

Query: 481 AKADLLVRVKSKASRKIICSTISNSGKLFAYSDHTKPNLFELKKSGGSKCSWTVNRRQLP 540
           AKADLLVRVKSKASRKIICS+ISNSGKLFAYSDH KPNL+ELKKSGGSKCSWTVNRRQLP
Sbjct: 481 AKADLLVRVKSKASRKIICSSISNSGKLFAYSDHAKPNLYELKKSGGSKCSWTVNRRQLP 540

Query: 541 NTLPFAHSMVFSFDSSRLIIAGHDKRIYVVDVGSLEVIHSFTPFRELQDDTLPPAEPPIT 600
           N LPFAHSMVFSFDSSRLIIAG+DKRIYVVD GSL+VIHSFTPFRELQDD LPPAEPPIT
Sbjct: 541 NMLPFAHSMVFSFDSSRLIIAGYDKRIYVVDTGSLKVIHSFTPFRELQDDALPPAEPPIT 600

Query: 601 KIFTSSDGQWLAAVNCFGDIYIFNMEIMRQHWFISRLDGASVTAGGFPQWNNNVLVVTTS 660
           KIFTSSDGQWLAAVNCFGDIY+FNMEIMRQHWFISRLDGAS+TAGGFPQWNNNVLVVTTS
Sbjct: 601 KIFTSSDGQWLAAVNCFGDIYVFNMEIMRQHWFISRLDGASITAGGFPQWNNNVLVVTTS 660

Query: 661 SNQVYAFDVEAKQLGDWSMRHTQALPKRFQEFPGEVIGLSFPPSATSLPVIVYSSRAMCL 720
           SNQVYAFDVEAKQLGDWSMRHTQALPKRFQEFPGEVIGLSFPPSATSLPVIVYSSRAMCL
Sbjct: 661 SNQVYAFDVEAKQLGDWSMRHTQALPKRFQEFPGEVIGLSFPPSATSLPVIVYSSRAMCL 720

Query: 721 IDFRMSVDQDDEYVMISGQDSTVKSLRGTPINGKLKRKLRDCQTEGRLHGRKNFEIFPFR 780
           IDF+MSVDQDDEYVMISGQDS VKSLRGTP+N KLKRKLRDCQT+    GRKNFEIFPFR
Sbjct: 721 IDFKMSVDQDDEYVMISGQDSMVKSLRGTPVNAKLKRKLRDCQTD----GRKNFEIFPFR 780

Query: 781 DPVLLIGHLSKTSLLIIEKPWLEVANTFDAPPVHRHIYGT 821
           DPVLLIGHLSKTS+LIIEKPWLEVANTFDAPPVHRHIYGT
Sbjct: 781 DPVLLIGHLSKTSVLIIEKPWLEVANTFDAPPVHRHIYGT 815

BLAST of Spg016355 vs. ExPASy TrEMBL
Match: A0A6J1FCB4 (U3 small nucleolar RNA-associated protein 4 homolog isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111442803 PE=4 SV=1)

HSP 1 Score: 1551.6 bits (4016), Expect = 0.0e+00
Identity = 751/820 (91.59%), Postives = 785/820 (95.73%), Query Frame = 0

Query: 1   MIEFYRNSSIQWKPSPVVALASSADDSQVAAAREDGSLEIWLVSPGSVGWHCQLTISGDP 60
           MIEFYRNSSI+WKPSPVVALASSAD SQVAAAREDGSLEIWLVSPGSVGWHCQLTI+GDP
Sbjct: 1   MIEFYRNSSIEWKPSPVVALASSADGSQVAAAREDGSLEIWLVSPGSVGWHCQLTINGDP 60

Query: 61  TSRVSSLVWCCAGSRDRPSGRLLSSSIDGSISEWDLFDLTQKTVLESIGVSIWQIAAAPC 120
           TSRVSSLVWC AGS+DRPSGRL SSSIDGSISEWDLF LTQK+VLESIG+SIWQIAAAPC
Sbjct: 61  TSRVSSLVWCRAGSKDRPSGRLFSSSIDGSISEWDLFGLTQKSVLESIGLSIWQIAAAPC 120

Query: 121 NIPEIHREVKPQDIENGHLTDSESDCQDCSESEDDSNSSEFRVQSSDVNPSLAIACDDGC 180
           N  E+HREVKP++IEN H+TD+ESDCQDCSESEDDSNSS+  VQ+SD +P LAIACDDGC
Sbjct: 121 NSREVHREVKPKEIENAHVTDNESDCQDCSESEDDSNSSKLHVQASDEDPCLAIACDDGC 180

Query: 181 VRIYNICDAEEFVYKRSLPRVSGKVLSVTWSNDAERIFSGSSDGFIRCWNASLGHEIYRI 240
           VRIYNICD EE +YKRSLPRVSG+VLSVTWSNDAERIFSGSSDGFIRCWNASLGHE+YRI
Sbjct: 181 VRIYNICDVEELIYKRSLPRVSGRVLSVTWSNDAERIFSGSSDGFIRCWNASLGHEVYRI 240

Query: 241 TAGLGGLGSGPELCVWSLLSLRCGTLVSADSSGSVQFWDSNHGTLLQAHTLHKGDVNALA 300
           TAGLGGLGSG ELCVWSLL LRCGTLVSADS+GSVQFWDSNHGTLLQAHTLHKGDVNALA
Sbjct: 241 TAGLGGLGSGSELCVWSLLFLRCGTLVSADSTGSVQFWDSNHGTLLQAHTLHKGDVNALA 300

Query: 301 VTPNHNRVYSAGSDGQVILYKLSNENVASSEDQNSSEVMKKWIYVGHVRAHTHDIRALTV 360
            TPNHNRVYSAGSDGQVILYKLSNENV SSED+ SSE+MKKWIYVGHVRAHTHDIRALTV
Sbjct: 301 ATPNHNRVYSAGSDGQVILYKLSNENVLSSEDKYSSEMMKKWIYVGHVRAHTHDIRALTV 360

Query: 361 AVPICREAELSQDEEVKRVRYKKKPNDFSYRKWAHLGVPMLISGGDDTKLFAYSAQEFTK 420
           AVPICRE E SQD EVKR+R+K+KPNDFSY KWAHLGVPML+SGGDDTKLFAYSAQEFTK
Sbjct: 361 AVPICRE-EASQDVEVKRIRHKRKPNDFSYHKWAHLGVPMLMSGGDDTKLFAYSAQEFTK 420

Query: 421 FSPHDICPAPQRVPMQLVLNTVFNQAPLLLVQAAHSLDILCVRPKSGSFRDMACGPSKGL 480
           FSPHDICPAPQRVPMQLVLNTVFNQAPLLLVQAAHSLDILCVRPK GSF D  CGPSKGL
Sbjct: 421 FSPHDICPAPQRVPMQLVLNTVFNQAPLLLVQAAHSLDILCVRPKCGSFGDKTCGPSKGL 480

Query: 481 AKADLLVRVKSKASRKIICSTISNSGKLFAYSDHTKPNLFELKKSGGSKCSWTVNRRQLP 540
           AKADLLVRVKSKASRKIICSTISNSGKLFA+SDH KPNL+ELKKSGGSKCSWTVNRR+LP
Sbjct: 481 AKADLLVRVKSKASRKIICSTISNSGKLFAFSDHAKPNLYELKKSGGSKCSWTVNRRKLP 540

Query: 541 NTLPFAHSMVFSFDSSRLIIAGHDKRIYVVDVGSLEVIHSFTPFRELQDDTLPPAEPPIT 600
           NTLPFAHSMVFSFDSSRLIIAGHDKRIYVVDV SLEV+HSFTPFRE QDD LPPAEPPIT
Sbjct: 541 NTLPFAHSMVFSFDSSRLIIAGHDKRIYVVDVSSLEVLHSFTPFREQQDDILPPAEPPIT 600

Query: 601 KIFTSSDGQWLAAVNCFGDIYIFNMEIMRQHWFISRLDGASVTAGGFPQWNNNVLVVTTS 660
           KIFTSSDGQWLAAVNCFGDIY+FNMEI+RQHWFISRLDGAS+TAGGFPQWNNNVLVVTTS
Sbjct: 601 KIFTSSDGQWLAAVNCFGDIYVFNMEILRQHWFISRLDGASITAGGFPQWNNNVLVVTTS 660

Query: 661 SNQVYAFDVEAKQLGDWSMRHTQALPKRFQEFPGEVIGLSFPPSATSLPVIVYSSRAMCL 720
           SNQ+YAFD+EAKQLGDWSMRHTQALPKRFQEFPGEVIGLSFPPS TSLPVIVYSSRAMCL
Sbjct: 661 SNQIYAFDIEAKQLGDWSMRHTQALPKRFQEFPGEVIGLSFPPSTTSLPVIVYSSRAMCL 720

Query: 721 IDFRMSVDQDDEYVMISGQDSTVKSLRGTPINGKLKRKLRDCQTEGRLHGRKNFEIFPFR 780
           IDFRMSVDQ+DEYVMISGQDSTVK+L GTPINGKLKRKLRDCQTEGR HGRKNFE FPFR
Sbjct: 721 IDFRMSVDQNDEYVMISGQDSTVKNLWGTPINGKLKRKLRDCQTEGRPHGRKNFENFPFR 780

Query: 781 DPVLLIGHLSKTSLLIIEKPWLEVANTFDAPPVHRHIYGT 821
           DPVLLIGHLSKTSLLIIEKPWLEV NTFDAPPVHRHIYGT
Sbjct: 781 DPVLLIGHLSKTSLLIIEKPWLEVVNTFDAPPVHRHIYGT 819

BLAST of Spg016355 vs. TAIR 10
Match: AT4G07410.1 (Transducin family protein / WD-40 repeat family protein )

HSP 1 Score: 1047.7 bits (2708), Expect = 4.8e-306
Identity = 531/830 (63.98%), Postives = 636/830 (76.63%), Query Frame = 0

Query: 5   YRNSSIQWKPSPVVALASSADDSQVAAAREDGSLEIWLVSPGSVGWHCQLTISGDPTSRV 64
           YR SS+ WKPSPVVALA+S+DDSQVAAAREDGSLEIWLVSPG+VGWHCQLTI GDP SR+
Sbjct: 4   YRCSSVDWKPSPVVALANSSDDSQVAAAREDGSLEIWLVSPGAVGWHCQLTIHGDPNSRI 63

Query: 65  SSLVWCCAGSRDRPSGRLLSSSIDGSISEWDLFDLTQKTVLESIGVSIWQIAAAPCNIPE 124
           SSL WCC+ S   PSGRL SSSIDGSISEWDLFDL QK VLESIG+SIWQ+A AP  I  
Sbjct: 64  SSLAWCCSPSIGLPSGRLFSSSIDGSISEWDLFDLKQKIVLESIGISIWQMALAP--ISG 123

Query: 125 IHREVKPQDIENGHLTDSESDCQDCSESEDDSNSSEFRVQS-SDVNPSLAIACDDGCVRI 184
              +V  + I+NG+L++  +D ++    ED S+S EF  +S  +++  LA ACDDGCVR+
Sbjct: 124 FSSDV--EGIKNGYLSEKSNDEEEIGSEEDGSDSDEFHEKSEEEIDRILAAACDDGCVRL 183

Query: 185 YNICDAEEFVYKRSLPRVSGKVLSVTWSNDAERIFSGSSDGFIRCWNASLGHEIYRITAG 244
           Y I + E+  Y RSLPRVSG+ LSVTWS DA+RIFSGSSDG IRCW+A+  HE+YRITAG
Sbjct: 184 YRISNLEKLTYYRSLPRVSGRALSVTWSPDAKRIFSGSSDGLIRCWDATSCHEVYRITAG 243

Query: 245 LGGLGSGPELCVWSLLSLRCGTLVSADSSGSVQFWDSNHGTLLQAHTLHKGDVNALAVTP 304
           LGGLGS  E+CVWSLLSLRC  LVS DS+G+VQFWDS HGTLL+AH+ HKGDVN LA  P
Sbjct: 244 LGGLGSSSEICVWSLLSLRCSVLVSGDSTGTVQFWDSEHGTLLEAHSNHKGDVNTLAAAP 303

Query: 305 NHNRVYSAGSDGQVILYKLSNENVASSEDQNSSEVMKKWIYVGHVRAHTHDIRALTVAVP 364
           +HNRV+SAG+DGQVILYKLS     S + + SS   +KW Y+G+V+AHTHDIRALTVAVP
Sbjct: 304 SHNRVFSAGADGQVILYKLSGSTNGSQDLKPSSS--QKWDYIGYVKAHTHDIRALTVAVP 363

Query: 365 ICRE----AELSQDEEVKRVRYKKKPNDFSYRKWAHLGVPMLISGGDDTKLFAYSAQEFT 424
           I RE     ++  D+  ++ R K KP DF+Y KWAHLGVPMLIS GDD KLFAYS QEFT
Sbjct: 364 ISREDPFPDDILPDKASRKHRKKGKPVDFTYHKWAHLGVPMLISAGDDAKLFAYSIQEFT 423

Query: 425 KFSPHDICPAPQRVPMQLVLNTVFNQAPLLLVQAAHSLDILCVRPKSGSFRDMACGPSKG 484
           KFSPHDICPAPQR+PMQ+V N++FN+  LLLVQ   +LDIL +   S          S G
Sbjct: 424 KFSPHDICPAPQRIPMQMVHNSMFNKTSLLLVQGISTLDILRLNISS---------DSSG 483

Query: 485 LAKADLLVRVKSKASRKIICSTISNSGKLFAYSDHTKPNLFELKKSGGSKCSWTVNRRQL 544
            A    LVRVKS+ +RKIICS ISN+G  FAYSD   P+LFELKK+  +KC W+V+RR+L
Sbjct: 484 RASTKSLVRVKSRDARKIICSAISNTGSHFAYSDQIGPSLFELKKNEFTKCPWSVSRRRL 543

Query: 545 PNTLPFAHSMVFSFDSSRLIIAGHDKRIYVVDVGSLEVIHSFTPFRELQDDTLP-PAEPP 604
           P  LPFAHSM+FS D SRLIIAGHD+RIY +D+ SLE++++FTP RE  +   P P EPP
Sbjct: 544 PE-LPFAHSMIFSSDCSRLIIAGHDRRIYTIDISSLELVYAFTPSREEHEGEAPTPKEPP 603

Query: 605 ITKIFTSSDGQWLAAVNCFGDIYIFNMEIMRQHWFISRLDGASVTAGGFPQWNNNVLVVT 664
           ITK+FTSSDGQWLAA+NCFGDIY+FN+E  RQHWFISRLDGASVTA GF  WNNN LV++
Sbjct: 604 ITKLFTSSDGQWLAAINCFGDIYVFNLETQRQHWFISRLDGASVTAAGFHPWNNNALVIS 663

Query: 665 TSSNQVYAFDVEAKQLGDWSMRHTQALPKRFQEFPGEVIGLSFPPSATSLPVIVYSSRAM 724
           TSSNQV+AFDVEA+QLG WS+ +T  LPKR+QEFPGEV+GLSF PS  S  VIVYSSRA 
Sbjct: 664 TSSNQVFAFDVEARQLGKWSLLNTYVLPKRYQEFPGEVLGLSFSPSPNSSSVIVYSSRAK 723

Query: 725 CLIDFRMSVDQDDEYVMISGQDSTVKSLRGTPIN-----GK---LKRKLRDCQTEGRLHG 784
           CLIDF   V++D+EY + +G  S  K+L G  +N     GK    KR+L + Q EG+ + 
Sbjct: 724 CLIDFGKPVEEDEEYDLPNGNLS--KTLEGKLVNLGLKKGKGTNRKRRLDEYQLEGKSNE 783

Query: 785 RKNFEIFPFRDPVLLIGHLSKTSLLIIEKPWLEVANTFDAPPVHRHIYGT 821
           RKNFEI P   PVL +GHLSK S+L+IEKPW++V  + D  PV RHI+GT
Sbjct: 784 RKNFEILPSNHPVLFVGHLSKNSILVIEKPWMDVVKSLDNQPVDRHIFGT 815

BLAST of Spg016355 vs. TAIR 10
Match: AT1G27470.1 (transducin family protein / WD-40 repeat family protein )

HSP 1 Score: 988.4 bits (2554), Expect = 3.4e-288
Identity = 510/826 (61.74%), Postives = 611/826 (73.97%), Query Frame = 0

Query: 5   YRNSSIQWKPSPVVALASSADDSQVAAAREDGSLEIWLVSPGSVGWHCQLTISGDPTSRV 64
           YR SSI WKPSPVVALA+SAD SQVAAAREDGSLE+WLVSPG+  WHCQL I GDP SR+
Sbjct: 4   YRCSSIDWKPSPVVALATSADGSQVAAAREDGSLELWLVSPGASSWHCQLIIHGDPKSRI 63

Query: 65  SSLVWCCAGSRDRPSGRLLSSSIDGSISEWDLFDLTQKTVLESIGVSIWQIAAAPCNIPE 124
           S L W   GS+   S RL SSSIDGSISEWDLFDL QK VL+SIG SIWQIA AP +I  
Sbjct: 64  SHLAWFGTGSKGSSSARLFSSSIDGSISEWDLFDLKQKVVLDSIGFSIWQIALAPISIEA 123

Query: 125 IHREVKPQDIENGHLTDSESDCQDCSESEDDSNSSEFRVQSSDVNPSLAIACDDGCVRIY 184
             +E K   I+NG+L++ +SD ++ S  EDDS   E   +   ++  LA ACDDGCVRIY
Sbjct: 124 ESKEAKL--IQNGYLSE-KSDDEEESGVEDDSELDELDEKVEALDRHLAAACDDGCVRIY 183

Query: 185 NICDAEEFVYKRSLPRVSGKVLSVTWSNDAERIFSGSSDGFIRCWNASLGHEIYRITAGL 244
            I ++++  Y RSLPRV+G+ LSVTWS DA+RIFSGSSDG IRCW+A+L HE+YRIT GL
Sbjct: 184 YISESDKLTYYRSLPRVTGRALSVTWSADAQRIFSGSSDGLIRCWDANLCHEVYRITVGL 243

Query: 245 GGLGSGPELCVWSLLSLRCGTLVSADSSGSVQFWDSNHGTLLQAHTLHKGDVNALAVTPN 304
           GGLG+G ELC+WSLLSLR G LVS DS+GSVQFWDS HGTLLQAH+ HKGDVNALA +P+
Sbjct: 244 GGLGNGSELCIWSLLSLRYGVLVSGDSTGSVQFWDSQHGTLLQAHSNHKGDVNALAASPS 303

Query: 305 HNRVYSAGSDGQVILYKLSNENVASSEDQNSSEVMKKWIYVGHVRAHTHDIRALTVAVPI 364
           HNRV+SAG+DGQVILYKLS     S + + SS   +KW Y+G VRAHTHDIRALTVAVPI
Sbjct: 304 HNRVFSAGADGQVILYKLSGGTSRSQDFKPSS--AQKWDYIGCVRAHTHDIRALTVAVPI 363

Query: 365 CREAELSQDEEVKRV---RYKKKPNDFSYRKWAHLGVPMLISGGDDTKLFAYSAQEFTKF 424
             E  +  D   K     + +K+P  FSY KWAHLGVPMLIS GDD KL+AYS QEFTKF
Sbjct: 364 SWEGSI-PDRNAKVTSPKQRRKEPAGFSYHKWAHLGVPMLISAGDDAKLYAYSVQEFTKF 423

Query: 425 SPHDICPAPQRVPMQLVLNTVFNQAPLLLVQAAHSLDILCVRPKSGSFRDMACGPSKGLA 484
            PHDICPAPQRVPMQ+V NTVFNQ  LLLVQ + SLDIL         R      S G  
Sbjct: 424 PPHDICPAPQRVPMQMVHNTVFNQTSLLLVQDSCSLDIL---------RIHISNDSSGRV 483

Query: 485 KADLLVRVKSKASRKIICSTISNSGKLFAYSDHTKPNLFELKKSGGSKCSWTVNRRQLPN 544
               LVRVKSK +RKIICS ISN+G LFAYSD  +P+LFELKK+   K  W+ NR++LPN
Sbjct: 484 STKPLVRVKSKDARKIICSAISNTGSLFAYSDQVRPSLFELKKNKIGKNPWSANRKRLPN 543

Query: 545 TLPFAHSMVFSFDSSRLIIAGHDKRIYVVDVGSLEVIHSFTPFRELQDDTLPPAEPPITK 604
            LPFAHSMVFS D SRLIIAGHD+RIY+VDVGS+E++HSFTP +E Q+   PP EPPITK
Sbjct: 544 -LPFAHSMVFSCDCSRLIIAGHDRRIYIVDVGSMELLHSFTPRQEGQEGESPPHEPPITK 603

Query: 605 IFTSSDGQWLAAVNCFGDIYIFNMEIMRQHWFISRLDGASVTAGGFPQWNNNVLVVTTSS 664
           ++TSS+  WLAA+NCFGDIY+FN+E  RQHWFISRLDGASV A GF   +NNV+V++TSS
Sbjct: 604 LYTSSNDDWLAAINCFGDIYVFNLETQRQHWFISRLDGASVAAAGFHPRDNNVIVISTSS 663

Query: 665 NQVYAFDVEAKQLGDWSMRHTQALPKRFQEFPGEVIGLSFPPSATSLPVIVYSSRAMCLI 724
           NQV+A DVEA++L  WS+  T  LPK +Q FPGEV+GLSF PS  S  VI+YSSRA CLI
Sbjct: 664 NQVFALDVEARELSKWSLLQTFCLPKSYQNFPGEVVGLSFSPSPCSSSVIIYSSRAKCLI 723

Query: 725 DFRMSVDQDDE-------YVMISGQDSTVKSLRGTPINGKLKRKLRDCQTEGRLHGRKNF 784
           +F    +QD++          + G+ +++    G   NG  KR+L + Q E + + RK F
Sbjct: 724 EFGKPAEQDEDTDTPCNLSEKLEGKLASISMKLG---NGAQKRRLEEYQKESKSNKRKKF 783

Query: 785 EIFPFRDPVLLIGHLSKTSLLIIEKPWLEVANTFDAPPVHRHIYGT 821
           E+   + PVL + HLSK+++L+IEKPW+EV    D  PVHRHI+GT
Sbjct: 784 EMVTSKHPVLYLRHLSKSAILVIEKPWMEVIKNLDTQPVHRHIFGT 810

BLAST of Spg016355 vs. TAIR 10
Match: AT4G07410.2 (Transducin family protein / WD-40 repeat family protein )

HSP 1 Score: 861.7 bits (2225), Expect = 4.8e-250
Identity = 435/703 (61.88%), Postives = 531/703 (75.53%), Query Frame = 0

Query: 132 QDIENGHLTDSESDCQDCSESEDDSNSSEFRVQS-SDVNPSLAIACDDGCVRIYNICDAE 191
           + I+NG+L++  +D ++    ED S+S EF  +S  +++  LA ACDDGCVR+Y I + E
Sbjct: 14  EGIKNGYLSEKSNDEEEIGSEEDGSDSDEFHEKSEEEIDRILAAACDDGCVRLYRISNLE 73

Query: 192 EFVYKRSLPRVSGKVLSVTWSNDAERIFSGSSDGFIRCWNASLGHEIYRITAGLGGLGSG 251
           +  Y RSLPRVSG+ LSVTWS DA+RIFSGSSDG IRCW+A+  HE+YRITAGLGGLGS 
Sbjct: 74  KLTYYRSLPRVSGRALSVTWSPDAKRIFSGSSDGLIRCWDATSCHEVYRITAGLGGLGSS 133

Query: 252 PELCVWSLLSLRCGTLVSADSSGSVQFWDSNHGTLLQAHTLHKGDVNALAVTPNHNRVYS 311
            E+CVWSLLSLRC  LVS DS+G+VQFWDS HGTLL+AH+ HKGDVN LA  P+HNRV+S
Sbjct: 134 SEICVWSLLSLRCSVLVSGDSTGTVQFWDSEHGTLLEAHSNHKGDVNTLAAAPSHNRVFS 193

Query: 312 AGSDGQVILYKLSNENVASSEDQNSSEVMKKWIYVGHVRAHTHDIRALTVAVPICRE--- 371
           AG+DGQVILYKLS     S + + SS   +KW Y+G+V+AHTHDIRALTVAVPI RE   
Sbjct: 194 AGADGQVILYKLSGSTNGSQDLKPSSS--QKWDYIGYVKAHTHDIRALTVAVPISREDPF 253

Query: 372 -AELSQDEEVKRVRYKKKPNDFSYRKWAHLGVPMLISGGDDTKLFAYSAQEFTKFSPHDI 431
             ++  D+  ++ R K KP DF+Y KWAHLGVPMLIS GDD KLFAYS QEFTKFSPHDI
Sbjct: 254 PDDILPDKASRKHRKKGKPVDFTYHKWAHLGVPMLISAGDDAKLFAYSIQEFTKFSPHDI 313

Query: 432 CPAPQRVPMQLVLNTVFNQAPLLLVQAAHSLDILCVRPKSGSFRDMACGPSKGLAKADLL 491
           CPAPQR+PMQ+V N++FN+  LLLVQ   +LDIL +   S          S G A    L
Sbjct: 314 CPAPQRIPMQMVHNSMFNKTSLLLVQGISTLDILRLNISS---------DSSGRASTKSL 373

Query: 492 VRVKSKASRKIICSTISNSGKLFAYSDHTKPNLFELKKSGGSKCSWTVNRRQLPNTLPFA 551
           VRVKS+ +RKIICS ISN+G  FAYSD   P+LFELKK+  +KC W+V+RR+LP  LPFA
Sbjct: 374 VRVKSRDARKIICSAISNTGSHFAYSDQIGPSLFELKKNEFTKCPWSVSRRRLPE-LPFA 433

Query: 552 HSMVFSFDSSRLIIAGHDKRIYVVDVGSLEVIHSFTPFRELQDDTLP-PAEPPITKIFTS 611
           HSM+FS D SRLIIAGHD+RIY +D+ SLE++++FTP RE  +   P P EPPITK+FTS
Sbjct: 434 HSMIFSSDCSRLIIAGHDRRIYTIDISSLELVYAFTPSREEHEGEAPTPKEPPITKLFTS 493

Query: 612 SDGQWLAAVNCFGDIYIFNMEIMRQHWFISRLDGASVTAGGFPQWNNNVLVVTTSSNQVY 671
           SDGQWLAA+NCFGDIY+FN+E  RQHWFISRLDGASVTA GF  WNNN LV++TSSNQV+
Sbjct: 494 SDGQWLAAINCFGDIYVFNLETQRQHWFISRLDGASVTAAGFHPWNNNALVISTSSNQVF 553

Query: 672 AFDVEAKQLGDWSMRHTQALPKRFQEFPGEVIGLSFPPSATSLPVIVYSSRAMCLIDFRM 731
           AFDVEA+QLG WS+ +T  LPKR+QEFPGEV+GLSF PS  S  VIVYSSRA CLIDF  
Sbjct: 554 AFDVEARQLGKWSLLNTYVLPKRYQEFPGEVLGLSFSPSPNSSSVIVYSSRAKCLIDFGK 613

Query: 732 SVDQDDEYVMISGQDSTVKSLRGTPIN-----GK---LKRKLRDCQTEGRLHGRKNFEIF 791
            V++D+EY + +G  S  K+L G  +N     GK    KR+L + Q EG+ + RKNFEI 
Sbjct: 614 PVEEDEEYDLPNGNLS--KTLEGKLVNLGLKKGKGTNRKRRLDEYQLEGKSNERKNFEIL 673

Query: 792 PFRDPVLLIGHLSKTSLLIIEKPWLEVANTFDAPPVHRHIYGT 821
           P   PVL +GHLSK S+L+IEKPW++V  + D  PV RHI+GT
Sbjct: 674 PSNHPVLFVGHLSKNSILVIEKPWMDVVKSLDNQPVDRHIFGT 702

BLAST of Spg016355 vs. TAIR 10
Match: AT4G34460.3 (GTP binding protein beta 1 )

HSP 1 Score: 47.8 bits (112), Expect = 5.0e-05
Identity = 39/146 (26.71%), Postives = 62/146 (42.47%), Query Frame = 0

Query: 189 AEEFVYKRSLPRVSGKVLSVTWSNDAERIFSGSSDGFIRCWNASLGHEIYRITAGLGGLG 248
           A + V  R+L   +GKV S+ W+ +  RI S S DG +  WNA    + + I        
Sbjct: 52  ATDLVCCRTLQGHTGKVYSLDWTPERNRIVSASQDGRLIVWNALTSQKTHAIKLP----- 111

Query: 249 SGPELCVWSLL--------SLRCGTLVSADSSGSVQFWDSNHGTLLQAHTL--HKGDVNA 308
                C W +         S+ CG L S  S  S+       GT+  +  L  H+G V+ 
Sbjct: 112 -----CAWVMTCAFSPNGQSVACGGLDSVCSIFSLSSTADKDGTVPVSRMLTGHRGYVSC 171

Query: 309 LAVTPNHN-RVYSAGSDGQVILYKLS 324
               PN +  + ++  D   IL+ ++
Sbjct: 172 CQYVPNEDAHLITSSGDQTCILWDVT 187

BLAST of Spg016355 vs. TAIR 10
Match: AT4G34460.1 (GTP binding protein beta 1 )

HSP 1 Score: 47.8 bits (112), Expect = 5.0e-05
Identity = 39/146 (26.71%), Postives = 62/146 (42.47%), Query Frame = 0

Query: 189 AEEFVYKRSLPRVSGKVLSVTWSNDAERIFSGSSDGFIRCWNASLGHEIYRITAGLGGLG 248
           A + V  R+L   +GKV S+ W+ +  RI S S DG +  WNA    + + I        
Sbjct: 52  ATDLVCCRTLQGHTGKVYSLDWTPERNRIVSASQDGRLIVWNALTSQKTHAIKLP----- 111

Query: 249 SGPELCVWSLL--------SLRCGTLVSADSSGSVQFWDSNHGTLLQAHTL--HKGDVNA 308
                C W +         S+ CG L S  S  S+       GT+  +  L  H+G V+ 
Sbjct: 112 -----CAWVMTCAFSPNGQSVACGGLDSVCSIFSLSSTADKDGTVPVSRMLTGHRGYVSC 171

Query: 309 LAVTPNHN-RVYSAGSDGQVILYKLS 324
               PN +  + ++  D   IL+ ++
Sbjct: 172 CQYVPNEDAHLITSSGDQTCILWDVT 187