Spg016232 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg016232 vs. NCBI nr
Match: XP_022141844.1 (U-box domain-containing protein 30-like [Momordica charantia])

HSP 1 Score: 817.4 bits (2110), Expect = 6.2e-233
Identity = 424/454 (93.39%), Postives = 437/454 (96.26%), Query Frame = 0

Query: 1   MPMYQPSGRRREADSELGVGLGGGGGQVLDVETAVKDGILGGGGGLICDGGGVEKLDLKE 60
           MPMYQPSGRRREADSELGV   GGGGQVLDVETAVKDGILGGGGGLICDGGG EKLDLKE
Sbjct: 1   MPMYQPSGRRREADSELGV---GGGGQVLDVETAVKDGILGGGGGLICDGGGAEKLDLKE 60

Query: 61  MVEELENIEVPSVFICPISLEPMRDPVTLCTGQTYERSNIRKWFSLGHFTCPTTMQELWD 120
           MVEELEN+EVPSVFICPISLEPMRDPVTLCTGQTYER+NI KWFSLGHFTCPTTMQELWD
Sbjct: 61  MVEELENLEVPSVFICPISLEPMRDPVTLCTGQTYERANILKWFSLGHFTCPTTMQELWD 120

Query: 121 DSITPNNTLHQLIYSWFSQKYLAMKKRSEDVQGRALELLDTLKKVKGQARVRALKELRQV 180
           DSITPNNTLHQLIYSWFSQKYLAMKKRSEDVQGRALELLDTLKKVKGQAR+RALKELRQV
Sbjct: 121 DSITPNNTLHQLIYSWFSQKYLAMKKRSEDVQGRALELLDTLKKVKGQARIRALKELRQV 180

Query: 181 VASNSSAKKTVEDNGGLALISSLLGPFTSHAVGSEAIGILVNLDLDGESRRNLVQPAKIS 240
           VASNSSAKKTVEDNGGLA ISSLLGPFTSHAVGSEAIGILVNL LD ESRRNLVQPAK+S
Sbjct: 181 VASNSSAKKTVEDNGGLASISSLLGPFTSHAVGSEAIGILVNLVLDSESRRNLVQPAKVS 240

Query: 241 LMVDMLNEGSSETKINCTGLIETLLEGDNCGVETVSSLSLLVGLLRLIKEKKHPNGVLAG 300
           LMVDMLNEGS+ETKINCT LIETLLEGD+CG E +SSLSLLVGLLRLIKEKKHPNGVLAG
Sbjct: 241 LMVDMLNEGSTETKINCTRLIETLLEGDDCGAENISSLSLLVGLLRLIKEKKHPNGVLAG 300

Query: 301 LRLLKALCSQEPVRNSVVSIGAIPQLIEILPNFDPDCLELALHVLDSLTSLSEGRVALKD 360
           LRLLK+LCSQEPV NSVVSIGAIPQLIEILPNFDP+CLELALHVLDSL+SLSEGRVALKD
Sbjct: 301 LRLLKSLCSQEPVWNSVVSIGAIPQLIEILPNFDPECLELALHVLDSLSSLSEGRVALKD 360

Query: 361 CPNTIPNLVKLLLRVSESCTQLALSILWAVCKLAPEECASQAVEAGLAAKLLL-IQCGCN 420
           CPNTIPNLVKLL+RVSESCTQLALSILWAVCKLAPEECA+QAVEAGLAAKLLL IQ GCN
Sbjct: 361 CPNTIPNLVKLLMRVSESCTQLALSILWAVCKLAPEECAAQAVEAGLAAKLLLVIQSGCN 420

Query: 421 PNLKQRSAELLKLCSLNYTATLFISKCKLTRTIQ 454
           P LKQR +ELLKLCSLNYTAT FISKCKLTRTIQ
Sbjct: 421 PVLKQRCSELLKLCSLNYTATHFISKCKLTRTIQ 451

BLAST of Spg016232 vs. NCBI nr
Match: XP_022981987.1 (U-box domain-containing protein 30-like [Cucurbita maxima] >XP_022981988.1 U-box domain-containing protein 30-like [Cucurbita maxima])

HSP 1 Score: 810.8 bits (2093), Expect = 5.8e-231
Identity = 427/456 (93.64%), Postives = 437/456 (95.83%), Query Frame = 0

Query: 1   MPMYQP-SGRRREADSELGV-GLGGGGGQVLDVETAVKDGILGGGGGLICDGGGVEKLDL 60
           MPMYQP SGRRREADSELG+ G GGGGGQVLDVETAVKDGIL GGGGLICDGGG EKLDL
Sbjct: 1   MPMYQPSSGRRREADSELGLGGGGGGGGQVLDVETAVKDGIL-GGGGLICDGGGAEKLDL 60

Query: 61  KEMVEELENIEVPSVFICPISLEPMRDPVTLCTGQTYERSNIRKWFSLGHFTCPTTMQEL 120
           KE+VEELENIEVPSVFICPISLEPMRDPVTLCTGQTYERSNI KWFSLGHFTCPTTMQEL
Sbjct: 61  KEIVEELENIEVPSVFICPISLEPMRDPVTLCTGQTYERSNILKWFSLGHFTCPTTMQEL 120

Query: 121 WDDSITPNNTLHQLIYSWFSQKYLAMKKRSEDVQGRALELLDTLKKVKGQARVRALKELR 180
           WDDSITPNNTLHQLIYSWF QKYLAMKKRSEDVQGRALELLDTLKKVKGQARVRALKELR
Sbjct: 121 WDDSITPNNTLHQLIYSWFWQKYLAMKKRSEDVQGRALELLDTLKKVKGQARVRALKELR 180

Query: 181 QVVASNSSAKKTVEDNGGLALISSLLGPFTSHAVGSEAIGILVNLDLDGESRRNLVQPAK 240
           QVVASNSSAKKTVEDNGGL  I SLLGPFTSHAVGSEAIGILVNLDLD ESRRNLVQPAK
Sbjct: 181 QVVASNSSAKKTVEDNGGLGSILSLLGPFTSHAVGSEAIGILVNLDLDAESRRNLVQPAK 240

Query: 241 ISLMVDMLNEGSSETKINCTGLIETLLEGDNCGVETVSSLSLLVGLLRLIKEKKHPNGVL 300
           ISLMVDMLNEGS+ETKINCT LIETLL+GD+CG +TVSS SLLVGLLRLIKEKKHPNGVL
Sbjct: 241 ISLMVDMLNEGSTETKINCTRLIETLLDGDDCGADTVSSFSLLVGLLRLIKEKKHPNGVL 300

Query: 301 AGLRLLKALCSQEPVRNSVVSIGAIPQLIEILPNFDPDCLELALHVLDSLTSLSEGRVAL 360
           AGLRLLKALC QEPVRNSVVSIGAIPQLIEILPNFDP+CLELALHVLDSL+SLSEGRVAL
Sbjct: 301 AGLRLLKALCCQEPVRNSVVSIGAIPQLIEILPNFDPECLELALHVLDSLSSLSEGRVAL 360

Query: 361 KDCPNTIPNLVKLLLRVSESCTQLALSILWAVCKLAPEECASQAVEAGLAAKLLL-IQCG 420
           KDCPNTI NLVKLL+R+SESCTQLALSILWAVCKLAPEECASQAVEAGLAAKLLL IQ G
Sbjct: 361 KDCPNTITNLVKLLMRISESCTQLALSILWAVCKLAPEECASQAVEAGLAAKLLLVIQSG 420

Query: 421 CNPNLKQRSAELLKLCSLNYTATLFISKCKLTRTIQ 454
           CNP LKQRSAELLKLCSLNYTATLFISKCKLTRTIQ
Sbjct: 421 CNPVLKQRSAELLKLCSLNYTATLFISKCKLTRTIQ 455

BLAST of Spg016232 vs. NCBI nr
Match: XP_022941310.1 (U-box domain-containing protein 30-like [Cucurbita moschata] >XP_022941311.1 U-box domain-containing protein 30-like [Cucurbita moschata])

HSP 1 Score: 810.8 bits (2093), Expect = 5.8e-231
Identity = 426/455 (93.63%), Postives = 437/455 (96.04%), Query Frame = 0

Query: 1   MPMYQP-SGRRREADSELGVGLGGGGGQVLDVETAVKDGILGGGGGLICDGGGVEKLDLK 60
           MPMYQP SGRRREADSE+G+G GGGGGQVLDVETAVKDGIL GGGGLICDGGG EKLDLK
Sbjct: 1   MPMYQPSSGRRREADSEMGLG-GGGGGQVLDVETAVKDGIL-GGGGLICDGGGAEKLDLK 60

Query: 61  EMVEELENIEVPSVFICPISLEPMRDPVTLCTGQTYERSNIRKWFSLGHFTCPTTMQELW 120
           E+VEELENIEVPSVFICPISLEPMRDPVTLCTGQTYERSNI KWFSLGHFTCPTTMQELW
Sbjct: 61  EIVEELENIEVPSVFICPISLEPMRDPVTLCTGQTYERSNILKWFSLGHFTCPTTMQELW 120

Query: 121 DDSITPNNTLHQLIYSWFSQKYLAMKKRSEDVQGRALELLDTLKKVKGQARVRALKELRQ 180
           DDSITPNNTLHQLIYSWF QKYLAMKKRSEDVQGRALELLDTLKKVKGQARVRALKELRQ
Sbjct: 121 DDSITPNNTLHQLIYSWFWQKYLAMKKRSEDVQGRALELLDTLKKVKGQARVRALKELRQ 180

Query: 181 VVASNSSAKKTVEDNGGLALISSLLGPFTSHAVGSEAIGILVNLDLDGESRRNLVQPAKI 240
           VVASNSSAKKTVEDNG L  I SLLGPFTSHAVGSEAIGILVNLDLD ESRRNLVQPAKI
Sbjct: 181 VVASNSSAKKTVEDNGVLGSILSLLGPFTSHAVGSEAIGILVNLDLDAESRRNLVQPAKI 240

Query: 241 SLMVDMLNEGSSETKINCTGLIETLLEGDNCGVETVSSLSLLVGLLRLIKEKKHPNGVLA 300
           SLMVDMLNEGS+ETKINCT LIETLL+GD+CG +TVSS SLLVGLLRLIKEKKHPNGVLA
Sbjct: 241 SLMVDMLNEGSTETKINCTRLIETLLDGDDCGADTVSSFSLLVGLLRLIKEKKHPNGVLA 300

Query: 301 GLRLLKALCSQEPVRNSVVSIGAIPQLIEILPNFDPDCLELALHVLDSLTSLSEGRVALK 360
           GLRLLKALC QEPVRNSVVSIGAIPQLIEILPNFDP+CLELALHVLDSL+SLSEGRVALK
Sbjct: 301 GLRLLKALCCQEPVRNSVVSIGAIPQLIEILPNFDPECLELALHVLDSLSSLSEGRVALK 360

Query: 361 DCPNTIPNLVKLLLRVSESCTQLALSILWAVCKLAPEECASQAVEAGLAAKLLL-IQCGC 420
           DCPNTIPNLVKLL+R+SESCTQLALSILWAVCKLAPEECASQAVEAGLAAKLLL IQ GC
Sbjct: 361 DCPNTIPNLVKLLMRISESCTQLALSILWAVCKLAPEECASQAVEAGLAAKLLLVIQSGC 420

Query: 421 NPNLKQRSAELLKLCSLNYTATLFISKCKLTRTIQ 454
           NP LKQRSAELLKLCSLNYTATLFISKCKLTRTIQ
Sbjct: 421 NPVLKQRSAELLKLCSLNYTATLFISKCKLTRTIQ 453

BLAST of Spg016232 vs. NCBI nr
Match: XP_023524197.1 (U-box domain-containing protein 30-like [Cucurbita pepo subsp. pepo] >XP_023524198.1 U-box domain-containing protein 30-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 810.8 bits (2093), Expect = 5.8e-231
Identity = 426/456 (93.42%), Postives = 437/456 (95.83%), Query Frame = 0

Query: 1   MPMYQP-SGRRREADSELGV-GLGGGGGQVLDVETAVKDGILGGGGGLICDGGGVEKLDL 60
           MPMYQP SGRRREADSE+G+ G GGGGGQVLDVETAVKDGIL GGGGLICDGGG EKLDL
Sbjct: 1   MPMYQPSSGRRREADSEMGLGGGGGGGGQVLDVETAVKDGIL-GGGGLICDGGGAEKLDL 60

Query: 61  KEMVEELENIEVPSVFICPISLEPMRDPVTLCTGQTYERSNIRKWFSLGHFTCPTTMQEL 120
           KE+VEELENIEVPSVFICPISLEPMRDPVTLCTGQTYERSNI KWFSLGHFTCPTTMQEL
Sbjct: 61  KEIVEELENIEVPSVFICPISLEPMRDPVTLCTGQTYERSNILKWFSLGHFTCPTTMQEL 120

Query: 121 WDDSITPNNTLHQLIYSWFSQKYLAMKKRSEDVQGRALELLDTLKKVKGQARVRALKELR 180
           WDDSITPNNTLHQLIYSWF QKYLAMKKRSEDVQGRALELLDTLKKVKGQARVRALKELR
Sbjct: 121 WDDSITPNNTLHQLIYSWFWQKYLAMKKRSEDVQGRALELLDTLKKVKGQARVRALKELR 180

Query: 181 QVVASNSSAKKTVEDNGGLALISSLLGPFTSHAVGSEAIGILVNLDLDGESRRNLVQPAK 240
           QVVASNSSAKKTVEDNGGL  I SLLGPFTSHAVGSEAIGILVNLDLD ESRRNLVQPAK
Sbjct: 181 QVVASNSSAKKTVEDNGGLGSILSLLGPFTSHAVGSEAIGILVNLDLDAESRRNLVQPAK 240

Query: 241 ISLMVDMLNEGSSETKINCTGLIETLLEGDNCGVETVSSLSLLVGLLRLIKEKKHPNGVL 300
           ISLMVDMLNEGS+ETKINCT LIETLL+GD+CG +TVSS SLLVGLLRLIKEKKHPNGVL
Sbjct: 241 ISLMVDMLNEGSTETKINCTRLIETLLDGDDCGADTVSSFSLLVGLLRLIKEKKHPNGVL 300

Query: 301 AGLRLLKALCSQEPVRNSVVSIGAIPQLIEILPNFDPDCLELALHVLDSLTSLSEGRVAL 360
           AGLRLLKALC QEPV NSVVSIGAIPQLIEILPNFDP+CLELALHVLDSL+SLSEGRVAL
Sbjct: 301 AGLRLLKALCCQEPVMNSVVSIGAIPQLIEILPNFDPECLELALHVLDSLSSLSEGRVAL 360

Query: 361 KDCPNTIPNLVKLLLRVSESCTQLALSILWAVCKLAPEECASQAVEAGLAAKLLL-IQCG 420
           KDCPNTIPNLVKLL+R+SESCTQLALSILWAVCKLAPEECASQAVEAGLAAKLLL IQ G
Sbjct: 361 KDCPNTIPNLVKLLMRISESCTQLALSILWAVCKLAPEECASQAVEAGLAAKLLLVIQSG 420

Query: 421 CNPNLKQRSAELLKLCSLNYTATLFISKCKLTRTIQ 454
           CNP LKQRSAELLKLCSLNYTATLFISKCKLTRTIQ
Sbjct: 421 CNPVLKQRSAELLKLCSLNYTATLFISKCKLTRTIQ 455

BLAST of Spg016232 vs. NCBI nr
Match: KAG7037754.1 (U-box domain-containing protein 30, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 809.7 bits (2090), Expect = 1.3e-230
Identity = 424/455 (93.19%), Postives = 435/455 (95.60%), Query Frame = 0

Query: 1   MPMYQP-SGRRREADSELGVGLGGGGGQVLDVETAVKDGILGGGGGLICDGGGVEKLDLK 60
           MPMYQP SGRRREADSE+G+G GGGGGQVLDVETAVKDGIL GGGGLICDGGG EKLDLK
Sbjct: 47  MPMYQPSSGRRREADSEMGLG-GGGGGQVLDVETAVKDGIL-GGGGLICDGGGAEKLDLK 106

Query: 61  EMVEELENIEVPSVFICPISLEPMRDPVTLCTGQTYERSNIRKWFSLGHFTCPTTMQELW 120
           E+VEELENIEVPSVFICPISLEPMRDPVTLCTGQTYERSNI KWFSLGHFTCPTTMQELW
Sbjct: 107 EIVEELENIEVPSVFICPISLEPMRDPVTLCTGQTYERSNILKWFSLGHFTCPTTMQELW 166

Query: 121 DDSITPNNTLHQLIYSWFSQKYLAMKKRSEDVQGRALELLDTLKKVKGQARVRALKELRQ 180
           DDSITPNNTLHQLIYSWF QKYLAMKKRSEDVQGRALELLDTLKKVKGQARVRALKELRQ
Sbjct: 167 DDSITPNNTLHQLIYSWFWQKYLAMKKRSEDVQGRALELLDTLKKVKGQARVRALKELRQ 226

Query: 181 VVASNSSAKKTVEDNGGLALISSLLGPFTSHAVGSEAIGILVNLDLDGESRRNLVQPAKI 240
           VV SNSSAKKTVEDNGGL  I SLLGPFTSHAVGSE IGILVNLDLD ESRRNLVQPAKI
Sbjct: 227 VVTSNSSAKKTVEDNGGLGSILSLLGPFTSHAVGSEVIGILVNLDLDAESRRNLVQPAKI 286

Query: 241 SLMVDMLNEGSSETKINCTGLIETLLEGDNCGVETVSSLSLLVGLLRLIKEKKHPNGVLA 300
           SLMVDMLNEGS+ETKINCT LIET L+GD+CG +TVSS SLLVGLLRLIKEKKHPNGVLA
Sbjct: 287 SLMVDMLNEGSTETKINCTRLIETFLDGDDCGADTVSSFSLLVGLLRLIKEKKHPNGVLA 346

Query: 301 GLRLLKALCSQEPVRNSVVSIGAIPQLIEILPNFDPDCLELALHVLDSLTSLSEGRVALK 360
           GLRLLKALC QEPVRNSVVSIGAIPQLIEILPNFDP+CLELALHVLDSL+SLSEGRVALK
Sbjct: 347 GLRLLKALCCQEPVRNSVVSIGAIPQLIEILPNFDPECLELALHVLDSLSSLSEGRVALK 406

Query: 361 DCPNTIPNLVKLLLRVSESCTQLALSILWAVCKLAPEECASQAVEAGLAAKLLL-IQCGC 420
           DCPNTIPNLVKLL+R+SESCTQLALSILWAVCKLAPEECASQAVEAGLAAKLLL IQ GC
Sbjct: 407 DCPNTIPNLVKLLMRISESCTQLALSILWAVCKLAPEECASQAVEAGLAAKLLLVIQSGC 466

Query: 421 NPNLKQRSAELLKLCSLNYTATLFISKCKLTRTIQ 454
           NP LKQRSAELLKLCSLNYTATLFISKCKLTRTIQ
Sbjct: 467 NPVLKQRSAELLKLCSLNYTATLFISKCKLTRTIQ 499

BLAST of Spg016232 vs. ExPASy Swiss-Prot
Match: Q058P4 (U-box domain-containing protein 30 OS=Arabidopsis thaliana OX=3702 GN=PUB30 PE=1 SV=1)

HSP 1 Score: 586.6 bits (1511), Expect = 2.3e-166
Identity = 308/458 (67.25%), Postives = 373/458 (81.44%), Query Frame = 0

Query: 1   MPMYQPSGRRREADSELGVGLGGGGGQVLDVETAVKDGILGGGGGLICDGGGV----EKL 60
           MPM+QP  R    D  +G   GGG GQVLD++TAVKDG+LGG      +GGGV    EKL
Sbjct: 1   MPMFQPLKR----DGLIGFE-GGGDGQVLDLDTAVKDGVLGG-----VNGGGVGVVDEKL 60

Query: 61  DLKEMVEELENIEVPSVFICPISLEPMRDPVTLCTGQTYERSNIRKWFSLGHFTCPTTMQ 120
           DLK+M++EL+  ++PSVFICPISLEPM+DPVTLCTGQTYER NI KWF+LGH TCPTTMQ
Sbjct: 61  DLKKMIKELDLQDIPSVFICPISLEPMQDPVTLCTGQTYERLNIHKWFNLGHLTCPTTMQ 120

Query: 121 ELWDDSITPNNTLHQLIYSWFSQKYLAMKKRSEDVQGRALELLDTLKKVKGQARVRALKE 180
           ELWDD++TPN TLH LIY+WFSQKY+ MKKRSEDVQGRA+E+L TLKK KGQARV AL E
Sbjct: 121 ELWDDTVTPNKTLHHLIYTWFSQKYVLMKKRSEDVQGRAIEILGTLKKAKGQARVHALSE 180

Query: 181 LRQVVASNSSAKKTVEDNGGLALISSLLGPFTSHAVGSEAIGILVNLDLDGESRRNLVQP 240
           L+Q+V ++  A+KTV + GG+++ISSLLGPFTSHAVGSE + ILV+LDLD +S+  L+QP
Sbjct: 181 LKQIVIAHLMARKTVVEEGGVSVISSLLGPFTSHAVGSEVVAILVSLDLDSDSKSGLMQP 240

Query: 241 AKISLMVDMLNEGSSETKINCTGLIETLLEGDNCGVETVSSLSLLVGLLRLIKEKKHPNG 300
           AK+SL+VDMLN+GS+ETKINC  LI  L+E      E VSS SLLVGL+RL+K+K+H NG
Sbjct: 241 AKVSLIVDMLNDGSNETKINCARLIRGLVEEKGFRAELVSSHSLLVGLMRLVKDKRHRNG 300

Query: 301 VLAGLRLLKALCSQEPVRNSVVSIGAIPQLIEILPNFDPDCLELALHVLDSLTSLSEGRV 360
           V   LRLLK +   + VR+ +VSIGA+PQL++ILP+ DP+CLELAL VLD+L +  EGRV
Sbjct: 301 VSPALRLLKPISVHKQVRSLMVSIGAVPQLVDILPSLDPECLELALFVLDALCTDVEGRV 360

Query: 361 ALKDCPNTIPNLVKLLLRVSESCTQLALSILWAVCKLAPEECASQAVEAGLAAKLLL-IQ 420
           A+KD  NTIP  V++L+RVSE+CT  ALSILW+VCKLAPEEC+  AVE GLAAKLLL IQ
Sbjct: 361 AVKDSANTIPYTVRVLMRVSENCTNYALSILWSVCKLAPEECSPLAVEVGLAAKLLLVIQ 420

Query: 421 CGCNPNLKQRSAELLKLCSLNYTATLFISKCKLTRTIQ 454
            GC+  LKQRSAELLKLCSL+Y+ T+FISKCKLTRTIQ
Sbjct: 421 SGCDAALKQRSAELLKLCSLHYSDTMFISKCKLTRTIQ 448

BLAST of Spg016232 vs. ExPASy Swiss-Prot
Match: Q9FHN9 (U-box domain-containing protein 31 OS=Arabidopsis thaliana OX=3702 GN=PUB31 PE=2 SV=1)

HSP 1 Score: 567.4 bits (1461), Expect = 1.5e-160
Identity = 300/456 (65.79%), Postives = 361/456 (79.17%), Query Frame = 0

Query: 1   MPMYQPSGRRREADSELGVGLGGGGGQVLDVETAVKDGILGGGGG--LICDGGGVEKLDL 60
           MPM+QPS              GG  G +LD+ +AVKDG+LGGG G  L+      +KLDL
Sbjct: 1   MPMFQPSKN------------GGFDGHILDLHSAVKDGVLGGGDGKFLVVVTDEKKKLDL 60

Query: 61  KEMVEELENIEVPSVFICPISLEPMRDPVTLCTGQTYERSNIRKWFSLGHFTCPTTMQEL 120
           KEM+ ELE  E+PSVFICPISLEPM+DPVTLCTGQTYERSNI KWF++GH TCPTTMQEL
Sbjct: 61  KEMISELELPEIPSVFICPISLEPMQDPVTLCTGQTYERSNILKWFNIGHCTCPTTMQEL 120

Query: 121 WDDSITPNNTLHQLIYSWFSQKYLAMKKRSEDVQGRALELLDTLKKVKGQARVRALKELR 180
           WDD +TPN TLHQLIY+WFSQKY+ MKKRSEDVQGRA+E+L TL+K KG+A+V AL EL+
Sbjct: 121 WDDLVTPNKTLHQLIYTWFSQKYVLMKKRSEDVQGRAIEILGTLRKAKGKAKVHALSELK 180

Query: 181 QVVASNSSAKKTVEDNGGLALISSLLGPFTSHAVGSEAIGILVNLDLDGESRRNLVQPAK 240
           QVV +++ AKKTV D GG+ +ISSLL PFTSHAVGSEAI ILVNL+LD +S+  L+QPA+
Sbjct: 181 QVVMAHAIAKKTVVDEGGVFVISSLLSPFTSHAVGSEAIAILVNLELDSDSKAGLMQPAR 240

Query: 241 ISLMVDMLNEGSSETKINCTGLIETLLEGDNCGVETVSSLSLLVGLLRLIKEKKHPNGVL 300
           +SLMVDMLN+GS ETKINC  LI  L+E      E VSS SLLVGL+RL+K+++  NGV 
Sbjct: 241 VSLMVDMLNDGSIETKINCARLIGRLVEEKGFRAELVSSHSLLVGLMRLVKDRRRRNGVS 300

Query: 301 AGLRLLKALCSQEPVRNSVVSIGAIPQLIEILPNFDPDCLELALHVLDSLTSLSEGRVAL 360
             L LLK++   + VRN +V IGA+PQL+++LP  D +CLE AL VLDSL   SEGR+AL
Sbjct: 301 PALTLLKSVSVHKQVRNLLVRIGAVPQLVDVLPCLDVECLESALFVLDSLCLESEGRIAL 360

Query: 361 KDCPNTIPNLVKLLLRVSESCTQLALSILWAVCKLAPEECASQAVEAGLAAKLLL-IQCG 420
           KD  NTIP+ V+LL++VSE CT  A+SILW+VCKLA EEC+S AVE GLAAKLLL IQ G
Sbjct: 361 KDSVNTIPHTVRLLMKVSEKCTNYAISILWSVCKLASEECSSLAVEVGLAAKLLLVIQSG 420

Query: 421 CNPNLKQRSAELLKLCSLNYTATLFISKCKLTRTIQ 454
           C+P LKQRSAELLKLCSL+Y+ ++FISKCKLTRTIQ
Sbjct: 421 CDPALKQRSAELLKLCSLHYSDSMFISKCKLTRTIQ 444

BLAST of Spg016232 vs. ExPASy Swiss-Prot
Match: Q10PI9 (U-box domain-containing protein 75 OS=Oryza sativa subsp. japonica OX=39947 GN=TUD1 PE=1 SV=1)

HSP 1 Score: 504.6 bits (1298), Expect = 1.2e-141
Identity = 260/450 (57.78%), Postives = 346/450 (76.89%), Query Frame = 0

Query: 25  GGQVLDVETAVKDGILGGG---GGLICDGGGVEKLDLKEMVEELE-------NIEVPSVF 84
           GGQVLD++TA+KDGILGGG   G      GG + ++L++M++EL+       +  VP+VF
Sbjct: 10  GGQVLDIDTALKDGILGGGPELGDAAAGDGGKQPVELRKMMDELDAAGDGGGDEAVPAVF 69

Query: 85  ICPISLEPMRDPVTLCTGQTYERSNIRKWFSLGHFTCPTTMQELWDDSITPNNTLHQLIY 144
           ICPISLEPM DPVTLCTGQTYE +NI +W +LGH TCPTTMQELWD +  PN TL QLI 
Sbjct: 70  ICPISLEPMVDPVTLCTGQTYESANISRWLALGHRTCPTTMQELWDVTPIPNTTLRQLIA 129

Query: 145 SWFSQKYLAMKKRSEDVQGRALELLDTLK--------KVKGQARVRALKELRQVVASNSS 204
           +WFS++Y   KKRS D  GRA EL+  L+         +KGQARV AL+ELR + A++ S
Sbjct: 130 AWFSRRYTRFKKRSADFHGRAAELVHALRGTAVPKRQPLKGQARVAALRELRSLAAAHQS 189

Query: 205 AKKTVEDNGGLALISSLLGPFTSHAVGSEAIGILVN-LDLDGESRRNLVQPAKISLMVDM 264
             K + + GG+ L++SLLGPFTSHAVGSEA+ ILV+ + LD +++  L+QPAK+SL+VDM
Sbjct: 190 VTKAIAEAGGVGLLTSLLGPFTSHAVGSEAVAILVSGVPLDADAKAALMQPAKVSLLVDM 249

Query: 265 LNEGSSETKINCTGLIETLLEGDNCGVETVSSLSLLVGLLRLIKEKKHPNGVLAGLRLLK 324
           LNEG+ +TKINC  LI  L+E      +TV+SLSLLVG++RL+++K+HP+GV AGL LL 
Sbjct: 250 LNEGAVDTKINCVRLIRILMEEKGFRPDTVASLSLLVGVMRLVRDKRHPDGVAAGLELLN 309

Query: 325 ALCS-QEPVRNSVVSIGAIPQLIEILPNFDPDCLELALHVLDSLTSLSEGRVALKDCPNT 384
           ++C+  +P R+ +VSIGA+PQL+E+LP    +C+E AL +LD+L ++ EGR+ALKDCP T
Sbjct: 310 SICAVHKPARSLIVSIGAVPQLVELLPELPTECVEPALDILDALAAVPEGRIALKDCPRT 369

Query: 385 IPNLVKLLLRVSESCTQLALSILWAVCKLAPEECASQAVEAGLAAKLLL-IQCGCNPNLK 444
           I N V+LL+RVSE+CT+ ALS+LW VC++APEECA  A++AGL AKLLL IQ GC P LK
Sbjct: 370 ITNAVRLLMRVSEACTRRALSMLWVVCRMAPEECAPAALDAGLGAKLLLVIQSGCGPELK 429

Query: 445 QRSAELLKLCSLNYTATLFISKCKLTRTIQ 454
           Q+++ELLKLC++N T+T+FISKCKLT+TIQ
Sbjct: 430 QQASELLKLCTMNCTSTVFISKCKLTKTIQ 459

BLAST of Spg016232 vs. ExPASy Swiss-Prot
Match: Q9LT79 (U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2 SV=1)

HSP 1 Score: 156.8 bits (395), Expect = 6.0e-37
Identity = 129/402 (32.09%), Postives = 201/402 (50.00%), Query Frame = 0

Query: 53  VEKLDLKEMVEELENIEVPSVFICPISLEPMRDPVTLCTGQTYERSNIRKWFSLG-HFTC 112
           +E LDL         I++P  F CPISLE M+DPVT+CTGQTY+R++I  W S+G + TC
Sbjct: 5   IEPLDL--------GIQIPYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTC 64

Query: 113 PTTMQELWDDSITPNNTLHQLIYSWF-------SQKYLAMKKRSEDVQGRALELLDTLKK 172
           P T   L D ++ PN+TL +LI  W         ++    K+ ++    RA  LL     
Sbjct: 65  PVTRAPLSDFTLIPNHTLRRLIQEWCVANRSNGVERIPTPKQPADPTSVRA--LLSQASA 124

Query: 173 VKG-----QARVRALKELRQVVASNSSAKKTV-EDNGGLALISSLLGPFTSHAVGSEAIG 232
           + G     ++R  AL+ LR     +   +  +   N    LI  L    TS  + SE++ 
Sbjct: 125 ITGTHVSVRSRAAALRRLRGFARDSDKNRVLIAAHNATEILIKILFSETTSSELVSESLA 184

Query: 233 ILVNLDLDGESRRNLV--QPAKISLMVDMLNEGSSETKINCTGLIETLLEGDNCG---VE 292
           +LV L +   ++   +   P ++  +  +L + S ET++N   LIE +  G         
Sbjct: 185 LLVMLPITEPNQFVSISSDPGRVEFLTRLLFDSSIETRVNAAALIEIVSTGTKSADLKGS 244

Query: 293 TVSSLSLLVGLLRLIKEKKHPNGVL-AGLRLLKALCSQEPVRNSVVSIGAIPQLIE-ILP 352
             +S S+  G+L L++        L  G++ L ALCS +  R+  ++ GA   LI+ +  
Sbjct: 245 ISNSESVFEGVLDLLRNPISSRRALKIGIKTLFALCSVKSTRHIAITAGAPEILIDRLAA 304

Query: 353 NFDPDCLELALHVLDSLTSLSEGRVALKDCPNTIPNLVKLLLRVSESCTQLALSILWAVC 412
           +FD    E AL  ++ L    EG  A  +   T+P LVK +LRVS+  T+ A   L A+C
Sbjct: 305 DFDRCDTERALATVELLCRTPEGCAAFGEHALTVPLLVKTILRVSDRATEYAAGALLALC 364

Query: 413 KLAPEECASQAVEAGLAAKLLL-IQCGCNPNLKQRSAELLKL 433
             A E    +A  AG+  +LLL +Q  C    K+++ +LLKL
Sbjct: 365 -TAEERWREEAAGAGVVVQLLLMVQSECTERAKKKAQKLLKL 395

BLAST of Spg016232 vs. ExPASy Swiss-Prot
Match: Q9FXA4 (U-box domain-containing protein 26 OS=Arabidopsis thaliana OX=3702 GN=PUB26 PE=2 SV=1)

HSP 1 Score: 154.5 bits (389), Expect = 3.0e-36
Identity = 124/406 (30.54%), Postives = 202/406 (49.75%), Query Frame = 0

Query: 51  GGVEKLDLKEMVEELENIEVPSVFICPISLEPMRDPVTLCTGQTYERSNIRKWFSLGHFT 110
           G +E LDL         I++P  F CPISL+ M DPVT+ TGQTY+R++I  W ++G+ T
Sbjct: 3   GNLEPLDL--------GIQIPYHFRCPISLDLMSDPVTISTGQTYDRTSIDSWIAMGNTT 62

Query: 111 CPTTMQELWDDSITPNNTLHQLIYSWF-------SQKYLAMKKRSEDVQGRALELLDTLK 170
           CP T   L D ++ PN+TL +LI  W         ++    K+ ++ +  R+  LL    
Sbjct: 63  CPVTRVALSDFTLIPNHTLRRLIQEWCVANRSNGVERIPTPKQPADPISVRS--LLSQAS 122

Query: 171 KVKG-----QARVRALKELRQVVASNSSAKKTVEDNGGLALISSLL-----GPFTSHAVG 230
            + G     ++R  A++ LR +   +   +  +  +    ++  +L         S  + 
Sbjct: 123 AITGTHVSVRSRAAAIRRLRGLARDSEKNRVLIAGHNAREILVRILFADIETTSLSSELV 182

Query: 231 SEAIGILVNLDL-DGESRRNLVQPAKISLMVDMLNEGSSETKINCTGLIETLLEGD---N 290
           SE++ +LV L + + E       P+++  M  +L + S E ++N   LIE +L G    +
Sbjct: 183 SESLALLVLLHMTETECEAVASDPSRVGFMTRLLFDSSIEIRVNAAALIEMVLTGAKSMD 242

Query: 291 CGVETVSSLSLLVGLLRLIKEKKHPNGVL-AGLRLLKALCSQEPVRNSVVSIGAIPQLIE 350
             +    S S+  G+L L+K        L  G++ + ALC  +  R+  +S GA   LI+
Sbjct: 243 LKLIISGSDSIFEGVLDLLKNPISSRRALKIGIKAIFALCLVKQTRHLAISAGAPGILID 302

Query: 351 -ILPNFDPDCLELALHVLDSLTSLSEGRVALKDCPNTIPNLVKLLLRVSESCTQLALSIL 410
            +  +FD    E  L  ++ L  L EG  A  +   T+P +VK +LRVS+  T+ A   L
Sbjct: 303 RLAADFDRCDTERGLATVELLCRLPEGCAAFGEHALTVPLMVKTILRVSDRATEYAAGAL 362

Query: 411 WAVCKLAPEECASQAVEAGLAAK-LLLIQCGCNPNLKQRSAELLKL 433
            A+C  A E C  +A  AGL  + LLL+Q  C    K+++  LLKL
Sbjct: 363 LALC-TAEERCRDEAAAAGLVTQLLLLVQSDCTERAKRKAQMLLKL 397

BLAST of Spg016232 vs. ExPASy TrEMBL
Match: A0A6J1CJY0 (RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC111012109 PE=4 SV=1)

HSP 1 Score: 817.4 bits (2110), Expect = 3.0e-233
Identity = 424/454 (93.39%), Postives = 437/454 (96.26%), Query Frame = 0

Query: 1   MPMYQPSGRRREADSELGVGLGGGGGQVLDVETAVKDGILGGGGGLICDGGGVEKLDLKE 60
           MPMYQPSGRRREADSELGV   GGGGQVLDVETAVKDGILGGGGGLICDGGG EKLDLKE
Sbjct: 1   MPMYQPSGRRREADSELGV---GGGGQVLDVETAVKDGILGGGGGLICDGGGAEKLDLKE 60

Query: 61  MVEELENIEVPSVFICPISLEPMRDPVTLCTGQTYERSNIRKWFSLGHFTCPTTMQELWD 120
           MVEELEN+EVPSVFICPISLEPMRDPVTLCTGQTYER+NI KWFSLGHFTCPTTMQELWD
Sbjct: 61  MVEELENLEVPSVFICPISLEPMRDPVTLCTGQTYERANILKWFSLGHFTCPTTMQELWD 120

Query: 121 DSITPNNTLHQLIYSWFSQKYLAMKKRSEDVQGRALELLDTLKKVKGQARVRALKELRQV 180
           DSITPNNTLHQLIYSWFSQKYLAMKKRSEDVQGRALELLDTLKKVKGQAR+RALKELRQV
Sbjct: 121 DSITPNNTLHQLIYSWFSQKYLAMKKRSEDVQGRALELLDTLKKVKGQARIRALKELRQV 180

Query: 181 VASNSSAKKTVEDNGGLALISSLLGPFTSHAVGSEAIGILVNLDLDGESRRNLVQPAKIS 240
           VASNSSAKKTVEDNGGLA ISSLLGPFTSHAVGSEAIGILVNL LD ESRRNLVQPAK+S
Sbjct: 181 VASNSSAKKTVEDNGGLASISSLLGPFTSHAVGSEAIGILVNLVLDSESRRNLVQPAKVS 240

Query: 241 LMVDMLNEGSSETKINCTGLIETLLEGDNCGVETVSSLSLLVGLLRLIKEKKHPNGVLAG 300
           LMVDMLNEGS+ETKINCT LIETLLEGD+CG E +SSLSLLVGLLRLIKEKKHPNGVLAG
Sbjct: 241 LMVDMLNEGSTETKINCTRLIETLLEGDDCGAENISSLSLLVGLLRLIKEKKHPNGVLAG 300

Query: 301 LRLLKALCSQEPVRNSVVSIGAIPQLIEILPNFDPDCLELALHVLDSLTSLSEGRVALKD 360
           LRLLK+LCSQEPV NSVVSIGAIPQLIEILPNFDP+CLELALHVLDSL+SLSEGRVALKD
Sbjct: 301 LRLLKSLCSQEPVWNSVVSIGAIPQLIEILPNFDPECLELALHVLDSLSSLSEGRVALKD 360

Query: 361 CPNTIPNLVKLLLRVSESCTQLALSILWAVCKLAPEECASQAVEAGLAAKLLL-IQCGCN 420
           CPNTIPNLVKLL+RVSESCTQLALSILWAVCKLAPEECA+QAVEAGLAAKLLL IQ GCN
Sbjct: 361 CPNTIPNLVKLLMRVSESCTQLALSILWAVCKLAPEECAAQAVEAGLAAKLLLVIQSGCN 420

Query: 421 PNLKQRSAELLKLCSLNYTATLFISKCKLTRTIQ 454
           P LKQR +ELLKLCSLNYTAT FISKCKLTRTIQ
Sbjct: 421 PVLKQRCSELLKLCSLNYTATHFISKCKLTRTIQ 451

BLAST of Spg016232 vs. ExPASy TrEMBL
Match: A0A6J1IVG5 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111480973 PE=4 SV=1)

HSP 1 Score: 810.8 bits (2093), Expect = 2.8e-231
Identity = 427/456 (93.64%), Postives = 437/456 (95.83%), Query Frame = 0

Query: 1   MPMYQP-SGRRREADSELGV-GLGGGGGQVLDVETAVKDGILGGGGGLICDGGGVEKLDL 60
           MPMYQP SGRRREADSELG+ G GGGGGQVLDVETAVKDGIL GGGGLICDGGG EKLDL
Sbjct: 1   MPMYQPSSGRRREADSELGLGGGGGGGGQVLDVETAVKDGIL-GGGGLICDGGGAEKLDL 60

Query: 61  KEMVEELENIEVPSVFICPISLEPMRDPVTLCTGQTYERSNIRKWFSLGHFTCPTTMQEL 120
           KE+VEELENIEVPSVFICPISLEPMRDPVTLCTGQTYERSNI KWFSLGHFTCPTTMQEL
Sbjct: 61  KEIVEELENIEVPSVFICPISLEPMRDPVTLCTGQTYERSNILKWFSLGHFTCPTTMQEL 120

Query: 121 WDDSITPNNTLHQLIYSWFSQKYLAMKKRSEDVQGRALELLDTLKKVKGQARVRALKELR 180
           WDDSITPNNTLHQLIYSWF QKYLAMKKRSEDVQGRALELLDTLKKVKGQARVRALKELR
Sbjct: 121 WDDSITPNNTLHQLIYSWFWQKYLAMKKRSEDVQGRALELLDTLKKVKGQARVRALKELR 180

Query: 181 QVVASNSSAKKTVEDNGGLALISSLLGPFTSHAVGSEAIGILVNLDLDGESRRNLVQPAK 240
           QVVASNSSAKKTVEDNGGL  I SLLGPFTSHAVGSEAIGILVNLDLD ESRRNLVQPAK
Sbjct: 181 QVVASNSSAKKTVEDNGGLGSILSLLGPFTSHAVGSEAIGILVNLDLDAESRRNLVQPAK 240

Query: 241 ISLMVDMLNEGSSETKINCTGLIETLLEGDNCGVETVSSLSLLVGLLRLIKEKKHPNGVL 300
           ISLMVDMLNEGS+ETKINCT LIETLL+GD+CG +TVSS SLLVGLLRLIKEKKHPNGVL
Sbjct: 241 ISLMVDMLNEGSTETKINCTRLIETLLDGDDCGADTVSSFSLLVGLLRLIKEKKHPNGVL 300

Query: 301 AGLRLLKALCSQEPVRNSVVSIGAIPQLIEILPNFDPDCLELALHVLDSLTSLSEGRVAL 360
           AGLRLLKALC QEPVRNSVVSIGAIPQLIEILPNFDP+CLELALHVLDSL+SLSEGRVAL
Sbjct: 301 AGLRLLKALCCQEPVRNSVVSIGAIPQLIEILPNFDPECLELALHVLDSLSSLSEGRVAL 360

Query: 361 KDCPNTIPNLVKLLLRVSESCTQLALSILWAVCKLAPEECASQAVEAGLAAKLLL-IQCG 420
           KDCPNTI NLVKLL+R+SESCTQLALSILWAVCKLAPEECASQAVEAGLAAKLLL IQ G
Sbjct: 361 KDCPNTITNLVKLLMRISESCTQLALSILWAVCKLAPEECASQAVEAGLAAKLLLVIQSG 420

Query: 421 CNPNLKQRSAELLKLCSLNYTATLFISKCKLTRTIQ 454
           CNP LKQRSAELLKLCSLNYTATLFISKCKLTRTIQ
Sbjct: 421 CNPVLKQRSAELLKLCSLNYTATLFISKCKLTRTIQ 455

BLAST of Spg016232 vs. ExPASy TrEMBL
Match: A0A6J1FKS4 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111446650 PE=4 SV=1)

HSP 1 Score: 810.8 bits (2093), Expect = 2.8e-231
Identity = 426/455 (93.63%), Postives = 437/455 (96.04%), Query Frame = 0

Query: 1   MPMYQP-SGRRREADSELGVGLGGGGGQVLDVETAVKDGILGGGGGLICDGGGVEKLDLK 60
           MPMYQP SGRRREADSE+G+G GGGGGQVLDVETAVKDGIL GGGGLICDGGG EKLDLK
Sbjct: 1   MPMYQPSSGRRREADSEMGLG-GGGGGQVLDVETAVKDGIL-GGGGLICDGGGAEKLDLK 60

Query: 61  EMVEELENIEVPSVFICPISLEPMRDPVTLCTGQTYERSNIRKWFSLGHFTCPTTMQELW 120
           E+VEELENIEVPSVFICPISLEPMRDPVTLCTGQTYERSNI KWFSLGHFTCPTTMQELW
Sbjct: 61  EIVEELENIEVPSVFICPISLEPMRDPVTLCTGQTYERSNILKWFSLGHFTCPTTMQELW 120

Query: 121 DDSITPNNTLHQLIYSWFSQKYLAMKKRSEDVQGRALELLDTLKKVKGQARVRALKELRQ 180
           DDSITPNNTLHQLIYSWF QKYLAMKKRSEDVQGRALELLDTLKKVKGQARVRALKELRQ
Sbjct: 121 DDSITPNNTLHQLIYSWFWQKYLAMKKRSEDVQGRALELLDTLKKVKGQARVRALKELRQ 180

Query: 181 VVASNSSAKKTVEDNGGLALISSLLGPFTSHAVGSEAIGILVNLDLDGESRRNLVQPAKI 240
           VVASNSSAKKTVEDNG L  I SLLGPFTSHAVGSEAIGILVNLDLD ESRRNLVQPAKI
Sbjct: 181 VVASNSSAKKTVEDNGVLGSILSLLGPFTSHAVGSEAIGILVNLDLDAESRRNLVQPAKI 240

Query: 241 SLMVDMLNEGSSETKINCTGLIETLLEGDNCGVETVSSLSLLVGLLRLIKEKKHPNGVLA 300
           SLMVDMLNEGS+ETKINCT LIETLL+GD+CG +TVSS SLLVGLLRLIKEKKHPNGVLA
Sbjct: 241 SLMVDMLNEGSTETKINCTRLIETLLDGDDCGADTVSSFSLLVGLLRLIKEKKHPNGVLA 300

Query: 301 GLRLLKALCSQEPVRNSVVSIGAIPQLIEILPNFDPDCLELALHVLDSLTSLSEGRVALK 360
           GLRLLKALC QEPVRNSVVSIGAIPQLIEILPNFDP+CLELALHVLDSL+SLSEGRVALK
Sbjct: 301 GLRLLKALCCQEPVRNSVVSIGAIPQLIEILPNFDPECLELALHVLDSLSSLSEGRVALK 360

Query: 361 DCPNTIPNLVKLLLRVSESCTQLALSILWAVCKLAPEECASQAVEAGLAAKLLL-IQCGC 420
           DCPNTIPNLVKLL+R+SESCTQLALSILWAVCKLAPEECASQAVEAGLAAKLLL IQ GC
Sbjct: 361 DCPNTIPNLVKLLMRISESCTQLALSILWAVCKLAPEECASQAVEAGLAAKLLLVIQSGC 420

Query: 421 NPNLKQRSAELLKLCSLNYTATLFISKCKLTRTIQ 454
           NP LKQRSAELLKLCSLNYTATLFISKCKLTRTIQ
Sbjct: 421 NPVLKQRSAELLKLCSLNYTATLFISKCKLTRTIQ 453

BLAST of Spg016232 vs. ExPASy TrEMBL
Match: A0A5A7VFI6 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold255G001750 PE=4 SV=1)

HSP 1 Score: 797.0 bits (2057), Expect = 4.2e-227
Identity = 418/457 (91.47%), Postives = 435/457 (95.19%), Query Frame = 0

Query: 1   MPMYQPSGRRREADSELGVGLGGGGGQVLDVETAVKDGIL---GGGGGLICDGGGVEKLD 60
           MPMYQPSGRRRE D+ELG+   GGGGQVLDVETAVKDGIL   GGGGGLIC+GG  EKLD
Sbjct: 1   MPMYQPSGRRREDDTELGL---GGGGQVLDVETAVKDGILGGGGGGGGLICEGGMTEKLD 60

Query: 61  LKEMVEELENIEVPSVFICPISLEPMRDPVTLCTGQTYERSNIRKWFSLGHFTCPTTMQE 120
           LKE+VEELENIEVPSVFICPISLEPMRDPVTLCTGQTYERSNI KWFSLGH TCPTTMQE
Sbjct: 61  LKEIVEELENIEVPSVFICPISLEPMRDPVTLCTGQTYERSNILKWFSLGHITCPTTMQE 120

Query: 121 LWDDSITPNNTLHQLIYSWFSQKYLAMKKRSEDVQGRALELLDTLKKVKGQARVRALKEL 180
           LWDDSITPNNTLHQLIYSWFSQKYLAMKKRSEDVQGRALELLDTLKKVKGQARVRALK+L
Sbjct: 121 LWDDSITPNNTLHQLIYSWFSQKYLAMKKRSEDVQGRALELLDTLKKVKGQARVRALKDL 180

Query: 181 RQVVASNSSAKKTVEDNGGLALISSLLGPFTSHAVGSEAIGILVNLDLDGESRRNLVQPA 240
           RQVVA N SAK TVEDNGG+ALISSLLGPFTSHAVGSEA+GILVNLDLD ESRRNLVQPA
Sbjct: 181 RQVVALN-SAKSTVEDNGGVALISSLLGPFTSHAVGSEALGILVNLDLDAESRRNLVQPA 240

Query: 241 KISLMVDMLNEGSSETKINCTGLIETLLEGDNCGVETVSSLSLLVGLLRLIKEKKHPNGV 300
           KISLMVDMLNEGS++TKINCT LIETLL GD+CG +TVSSLSLLVGLLRLIKEKKH NGV
Sbjct: 241 KISLMVDMLNEGSTDTKINCTRLIETLLNGDDCGTDTVSSLSLLVGLLRLIKEKKHQNGV 300

Query: 301 LAGLRLLKALCSQEPVRNSVVSIGAIPQLIEILPNFDPDCLELALHVLDSLTSLSEGRVA 360
           LAGLRLLKALCSQEPVRNS+VSIGAIPQLIEILPNFDP+CLELALHVLDSL+SLSEGRVA
Sbjct: 301 LAGLRLLKALCSQEPVRNSLVSIGAIPQLIEILPNFDPECLELALHVLDSLSSLSEGRVA 360

Query: 361 LKDCPNTIPNLVKLLLRVSESCTQLALSILWAVCKLAPEECASQAVEAGLAAKLLL-IQC 420
           LKDCPNTIPNLVKLL+R+SESCTQLALSILW+VCKLAPEECASQAVEAGLAAKLLL IQ 
Sbjct: 361 LKDCPNTIPNLVKLLMRISESCTQLALSILWSVCKLAPEECASQAVEAGLAAKLLLVIQS 420

Query: 421 GCNPNLKQRSAELLKLCSLNYTATLFISKCKLTRTIQ 454
           GCNP LKQRSAELLKLCSLNYTATLFISKCKLTRTIQ
Sbjct: 421 GCNPILKQRSAELLKLCSLNYTATLFISKCKLTRTIQ 453

BLAST of Spg016232 vs. ExPASy TrEMBL
Match: A0A1S3BTT1 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103493618 PE=4 SV=1)

HSP 1 Score: 797.0 bits (2057), Expect = 4.2e-227
Identity = 418/457 (91.47%), Postives = 435/457 (95.19%), Query Frame = 0

Query: 1   MPMYQPSGRRREADSELGVGLGGGGGQVLDVETAVKDGIL---GGGGGLICDGGGVEKLD 60
           MPMYQPSGRRRE D+ELG+   GGGGQVLDVETAVKDGIL   GGGGGLIC+GG  EKLD
Sbjct: 1   MPMYQPSGRRREDDTELGL---GGGGQVLDVETAVKDGILGGGGGGGGLICEGGMTEKLD 60

Query: 61  LKEMVEELENIEVPSVFICPISLEPMRDPVTLCTGQTYERSNIRKWFSLGHFTCPTTMQE 120
           LKE+VEELENIEVPSVFICPISLEPMRDPVTLCTGQTYERSNI KWFSLGH TCPTTMQE
Sbjct: 61  LKEIVEELENIEVPSVFICPISLEPMRDPVTLCTGQTYERSNILKWFSLGHITCPTTMQE 120

Query: 121 LWDDSITPNNTLHQLIYSWFSQKYLAMKKRSEDVQGRALELLDTLKKVKGQARVRALKEL 180
           LWDDSITPNNTLHQLIYSWFSQKYLAMKKRSEDVQGRALELLDTLKKVKGQARVRALK+L
Sbjct: 121 LWDDSITPNNTLHQLIYSWFSQKYLAMKKRSEDVQGRALELLDTLKKVKGQARVRALKDL 180

Query: 181 RQVVASNSSAKKTVEDNGGLALISSLLGPFTSHAVGSEAIGILVNLDLDGESRRNLVQPA 240
           RQVVA N SAK TVEDNGG+ALISSLLGPFTSHAVGSEA+GILVNLDLD ESRRNLVQPA
Sbjct: 181 RQVVALN-SAKSTVEDNGGVALISSLLGPFTSHAVGSEALGILVNLDLDAESRRNLVQPA 240

Query: 241 KISLMVDMLNEGSSETKINCTGLIETLLEGDNCGVETVSSLSLLVGLLRLIKEKKHPNGV 300
           KISLMVDMLNEGS++TKINCT LIETLL GD+CG +TVSSLSLLVGLLRLIKEKKH NGV
Sbjct: 241 KISLMVDMLNEGSTDTKINCTRLIETLLNGDDCGTDTVSSLSLLVGLLRLIKEKKHQNGV 300

Query: 301 LAGLRLLKALCSQEPVRNSVVSIGAIPQLIEILPNFDPDCLELALHVLDSLTSLSEGRVA 360
           LAGLRLLKALCSQEPVRNS+VSIGAIPQLIEILPNFDP+CLELALHVLDSL+SLSEGRVA
Sbjct: 301 LAGLRLLKALCSQEPVRNSLVSIGAIPQLIEILPNFDPECLELALHVLDSLSSLSEGRVA 360

Query: 361 LKDCPNTIPNLVKLLLRVSESCTQLALSILWAVCKLAPEECASQAVEAGLAAKLLL-IQC 420
           LKDCPNTIPNLVKLL+R+SESCTQLALSILW+VCKLAPEECASQAVEAGLAAKLLL IQ 
Sbjct: 361 LKDCPNTIPNLVKLLMRISESCTQLALSILWSVCKLAPEECASQAVEAGLAAKLLLVIQS 420

Query: 421 GCNPNLKQRSAELLKLCSLNYTATLFISKCKLTRTIQ 454
           GCNP LKQRSAELLKLCSLNYTATLFISKCKLTRTIQ
Sbjct: 421 GCNPILKQRSAELLKLCSLNYTATLFISKCKLTRTIQ 453

BLAST of Spg016232 vs. TAIR 10
Match: AT3G49810.1 (ARM repeat superfamily protein )

HSP 1 Score: 586.6 bits (1511), Expect = 1.7e-167
Identity = 308/458 (67.25%), Postives = 373/458 (81.44%), Query Frame = 0

Query: 1   MPMYQPSGRRREADSELGVGLGGGGGQVLDVETAVKDGILGGGGGLICDGGGV----EKL 60
           MPM+QP  R    D  +G   GGG GQVLD++TAVKDG+LGG      +GGGV    EKL
Sbjct: 1   MPMFQPLKR----DGLIGFE-GGGDGQVLDLDTAVKDGVLGG-----VNGGGVGVVDEKL 60

Query: 61  DLKEMVEELENIEVPSVFICPISLEPMRDPVTLCTGQTYERSNIRKWFSLGHFTCPTTMQ 120
           DLK+M++EL+  ++PSVFICPISLEPM+DPVTLCTGQTYER NI KWF+LGH TCPTTMQ
Sbjct: 61  DLKKMIKELDLQDIPSVFICPISLEPMQDPVTLCTGQTYERLNIHKWFNLGHLTCPTTMQ 120

Query: 121 ELWDDSITPNNTLHQLIYSWFSQKYLAMKKRSEDVQGRALELLDTLKKVKGQARVRALKE 180
           ELWDD++TPN TLH LIY+WFSQKY+ MKKRSEDVQGRA+E+L TLKK KGQARV AL E
Sbjct: 121 ELWDDTVTPNKTLHHLIYTWFSQKYVLMKKRSEDVQGRAIEILGTLKKAKGQARVHALSE 180

Query: 181 LRQVVASNSSAKKTVEDNGGLALISSLLGPFTSHAVGSEAIGILVNLDLDGESRRNLVQP 240
           L+Q+V ++  A+KTV + GG+++ISSLLGPFTSHAVGSE + ILV+LDLD +S+  L+QP
Sbjct: 181 LKQIVIAHLMARKTVVEEGGVSVISSLLGPFTSHAVGSEVVAILVSLDLDSDSKSGLMQP 240

Query: 241 AKISLMVDMLNEGSSETKINCTGLIETLLEGDNCGVETVSSLSLLVGLLRLIKEKKHPNG 300
           AK+SL+VDMLN+GS+ETKINC  LI  L+E      E VSS SLLVGL+RL+K+K+H NG
Sbjct: 241 AKVSLIVDMLNDGSNETKINCARLIRGLVEEKGFRAELVSSHSLLVGLMRLVKDKRHRNG 300

Query: 301 VLAGLRLLKALCSQEPVRNSVVSIGAIPQLIEILPNFDPDCLELALHVLDSLTSLSEGRV 360
           V   LRLLK +   + VR+ +VSIGA+PQL++ILP+ DP+CLELAL VLD+L +  EGRV
Sbjct: 301 VSPALRLLKPISVHKQVRSLMVSIGAVPQLVDILPSLDPECLELALFVLDALCTDVEGRV 360

Query: 361 ALKDCPNTIPNLVKLLLRVSESCTQLALSILWAVCKLAPEECASQAVEAGLAAKLLL-IQ 420
           A+KD  NTIP  V++L+RVSE+CT  ALSILW+VCKLAPEEC+  AVE GLAAKLLL IQ
Sbjct: 361 AVKDSANTIPYTVRVLMRVSENCTNYALSILWSVCKLAPEECSPLAVEVGLAAKLLLVIQ 420

Query: 421 CGCNPNLKQRSAELLKLCSLNYTATLFISKCKLTRTIQ 454
            GC+  LKQRSAELLKLCSL+Y+ T+FISKCKLTRTIQ
Sbjct: 421 SGCDAALKQRSAELLKLCSLHYSDTMFISKCKLTRTIQ 448

BLAST of Spg016232 vs. TAIR 10
Match: AT5G65920.1 (ARM repeat superfamily protein )

HSP 1 Score: 567.4 bits (1461), Expect = 1.0e-161
Identity = 300/456 (65.79%), Postives = 361/456 (79.17%), Query Frame = 0

Query: 1   MPMYQPSGRRREADSELGVGLGGGGGQVLDVETAVKDGILGGGGG--LICDGGGVEKLDL 60
           MPM+QPS              GG  G +LD+ +AVKDG+LGGG G  L+      +KLDL
Sbjct: 1   MPMFQPSKN------------GGFDGHILDLHSAVKDGVLGGGDGKFLVVVTDEKKKLDL 60

Query: 61  KEMVEELENIEVPSVFICPISLEPMRDPVTLCTGQTYERSNIRKWFSLGHFTCPTTMQEL 120
           KEM+ ELE  E+PSVFICPISLEPM+DPVTLCTGQTYERSNI KWF++GH TCPTTMQEL
Sbjct: 61  KEMISELELPEIPSVFICPISLEPMQDPVTLCTGQTYERSNILKWFNIGHCTCPTTMQEL 120

Query: 121 WDDSITPNNTLHQLIYSWFSQKYLAMKKRSEDVQGRALELLDTLKKVKGQARVRALKELR 180
           WDD +TPN TLHQLIY+WFSQKY+ MKKRSEDVQGRA+E+L TL+K KG+A+V AL EL+
Sbjct: 121 WDDLVTPNKTLHQLIYTWFSQKYVLMKKRSEDVQGRAIEILGTLRKAKGKAKVHALSELK 180

Query: 181 QVVASNSSAKKTVEDNGGLALISSLLGPFTSHAVGSEAIGILVNLDLDGESRRNLVQPAK 240
           QVV +++ AKKTV D GG+ +ISSLL PFTSHAVGSEAI ILVNL+LD +S+  L+QPA+
Sbjct: 181 QVVMAHAIAKKTVVDEGGVFVISSLLSPFTSHAVGSEAIAILVNLELDSDSKAGLMQPAR 240

Query: 241 ISLMVDMLNEGSSETKINCTGLIETLLEGDNCGVETVSSLSLLVGLLRLIKEKKHPNGVL 300
           +SLMVDMLN+GS ETKINC  LI  L+E      E VSS SLLVGL+RL+K+++  NGV 
Sbjct: 241 VSLMVDMLNDGSIETKINCARLIGRLVEEKGFRAELVSSHSLLVGLMRLVKDRRRRNGVS 300

Query: 301 AGLRLLKALCSQEPVRNSVVSIGAIPQLIEILPNFDPDCLELALHVLDSLTSLSEGRVAL 360
             L LLK++   + VRN +V IGA+PQL+++LP  D +CLE AL VLDSL   SEGR+AL
Sbjct: 301 PALTLLKSVSVHKQVRNLLVRIGAVPQLVDVLPCLDVECLESALFVLDSLCLESEGRIAL 360

Query: 361 KDCPNTIPNLVKLLLRVSESCTQLALSILWAVCKLAPEECASQAVEAGLAAKLLL-IQCG 420
           KD  NTIP+ V+LL++VSE CT  A+SILW+VCKLA EEC+S AVE GLAAKLLL IQ G
Sbjct: 361 KDSVNTIPHTVRLLMKVSEKCTNYAISILWSVCKLASEECSSLAVEVGLAAKLLLVIQSG 420

Query: 421 CNPNLKQRSAELLKLCSLNYTATLFISKCKLTRTIQ 454
           C+P LKQRSAELLKLCSL+Y+ ++FISKCKLTRTIQ
Sbjct: 421 CDPALKQRSAELLKLCSLHYSDSMFISKCKLTRTIQ 444

BLAST of Spg016232 vs. TAIR 10
Match: AT3G19380.1 (plant U-box 25 )

HSP 1 Score: 156.8 bits (395), Expect = 4.2e-38
Identity = 129/402 (32.09%), Postives = 201/402 (50.00%), Query Frame = 0

Query: 53  VEKLDLKEMVEELENIEVPSVFICPISLEPMRDPVTLCTGQTYERSNIRKWFSLG-HFTC 112
           +E LDL         I++P  F CPISLE M+DPVT+CTGQTY+R++I  W S+G + TC
Sbjct: 5   IEPLDL--------GIQIPYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTC 64

Query: 113 PTTMQELWDDSITPNNTLHQLIYSWF-------SQKYLAMKKRSEDVQGRALELLDTLKK 172
           P T   L D ++ PN+TL +LI  W         ++    K+ ++    RA  LL     
Sbjct: 65  PVTRAPLSDFTLIPNHTLRRLIQEWCVANRSNGVERIPTPKQPADPTSVRA--LLSQASA 124

Query: 173 VKG-----QARVRALKELRQVVASNSSAKKTV-EDNGGLALISSLLGPFTSHAVGSEAIG 232
           + G     ++R  AL+ LR     +   +  +   N    LI  L    TS  + SE++ 
Sbjct: 125 ITGTHVSVRSRAAALRRLRGFARDSDKNRVLIAAHNATEILIKILFSETTSSELVSESLA 184

Query: 233 ILVNLDLDGESRRNLV--QPAKISLMVDMLNEGSSETKINCTGLIETLLEGDNCG---VE 292
           +LV L +   ++   +   P ++  +  +L + S ET++N   LIE +  G         
Sbjct: 185 LLVMLPITEPNQFVSISSDPGRVEFLTRLLFDSSIETRVNAAALIEIVSTGTKSADLKGS 244

Query: 293 TVSSLSLLVGLLRLIKEKKHPNGVL-AGLRLLKALCSQEPVRNSVVSIGAIPQLIE-ILP 352
             +S S+  G+L L++        L  G++ L ALCS +  R+  ++ GA   LI+ +  
Sbjct: 245 ISNSESVFEGVLDLLRNPISSRRALKIGIKTLFALCSVKSTRHIAITAGAPEILIDRLAA 304

Query: 353 NFDPDCLELALHVLDSLTSLSEGRVALKDCPNTIPNLVKLLLRVSESCTQLALSILWAVC 412
           +FD    E AL  ++ L    EG  A  +   T+P LVK +LRVS+  T+ A   L A+C
Sbjct: 305 DFDRCDTERALATVELLCRTPEGCAAFGEHALTVPLLVKTILRVSDRATEYAAGALLALC 364

Query: 413 KLAPEECASQAVEAGLAAKLLL-IQCGCNPNLKQRSAELLKL 433
             A E    +A  AG+  +LLL +Q  C    K+++ +LLKL
Sbjct: 365 -TAEERWREEAAGAGVVVQLLLMVQSECTERAKKKAQKLLKL 395

BLAST of Spg016232 vs. TAIR 10
Match: AT1G49780.1 (plant U-box 26 )

HSP 1 Score: 154.5 bits (389), Expect = 2.1e-37
Identity = 124/406 (30.54%), Postives = 202/406 (49.75%), Query Frame = 0

Query: 51  GGVEKLDLKEMVEELENIEVPSVFICPISLEPMRDPVTLCTGQTYERSNIRKWFSLGHFT 110
           G +E LDL         I++P  F CPISL+ M DPVT+ TGQTY+R++I  W ++G+ T
Sbjct: 3   GNLEPLDL--------GIQIPYHFRCPISLDLMSDPVTISTGQTYDRTSIDSWIAMGNTT 62

Query: 111 CPTTMQELWDDSITPNNTLHQLIYSWF-------SQKYLAMKKRSEDVQGRALELLDTLK 170
           CP T   L D ++ PN+TL +LI  W         ++    K+ ++ +  R+  LL    
Sbjct: 63  CPVTRVALSDFTLIPNHTLRRLIQEWCVANRSNGVERIPTPKQPADPISVRS--LLSQAS 122

Query: 171 KVKG-----QARVRALKELRQVVASNSSAKKTVEDNGGLALISSLL-----GPFTSHAVG 230
            + G     ++R  A++ LR +   +   +  +  +    ++  +L         S  + 
Sbjct: 123 AITGTHVSVRSRAAAIRRLRGLARDSEKNRVLIAGHNAREILVRILFADIETTSLSSELV 182

Query: 231 SEAIGILVNLDL-DGESRRNLVQPAKISLMVDMLNEGSSETKINCTGLIETLLEGD---N 290
           SE++ +LV L + + E       P+++  M  +L + S E ++N   LIE +L G    +
Sbjct: 183 SESLALLVLLHMTETECEAVASDPSRVGFMTRLLFDSSIEIRVNAAALIEMVLTGAKSMD 242

Query: 291 CGVETVSSLSLLVGLLRLIKEKKHPNGVL-AGLRLLKALCSQEPVRNSVVSIGAIPQLIE 350
             +    S S+  G+L L+K        L  G++ + ALC  +  R+  +S GA   LI+
Sbjct: 243 LKLIISGSDSIFEGVLDLLKNPISSRRALKIGIKAIFALCLVKQTRHLAISAGAPGILID 302

Query: 351 -ILPNFDPDCLELALHVLDSLTSLSEGRVALKDCPNTIPNLVKLLLRVSESCTQLALSIL 410
            +  +FD    E  L  ++ L  L EG  A  +   T+P +VK +LRVS+  T+ A   L
Sbjct: 303 RLAADFDRCDTERGLATVELLCRLPEGCAAFGEHALTVPLMVKTILRVSDRATEYAAGAL 362

Query: 411 WAVCKLAPEECASQAVEAGLAAK-LLLIQCGCNPNLKQRSAELLKL 433
            A+C  A E C  +A  AGL  + LLL+Q  C    K+++  LLKL
Sbjct: 363 LALC-TAEERCRDEAAAAGLVTQLLLLVQSDCTERAKRKAQMLLKL 397

BLAST of Spg016232 vs. TAIR 10
Match: AT2G35930.1 (plant U-box 23 )

HSP 1 Score: 143.7 bits (361), Expect = 3.7e-34
Identity = 108/383 (28.20%), Postives = 197/383 (51.44%), Query Frame = 0

Query: 66  ENIEVPSVFICPISLEPMRDPVTLCTGQTYERSNIRKWFSLG-HFTCPTTMQELWDDSIT 125
           E IE+P  F+CPISLE M+DPV + TG TY+R +I KW   G   +CP T Q++ D  +T
Sbjct: 8   EEIEIPPFFLCPISLEIMKDPVIVSTGITYDRDSIEKWLFAGKKNSCPVTKQDITDADLT 67

Query: 126 PNNTLHQLIYSWFSQKYLAMKKR----SEDVQGRALELLDTLKKVKGQARVRALKELRQV 185
           PN+TL +LI SW +       +R       +    +E L        + +V+ LK LRQ+
Sbjct: 68  PNHTLRRLIQSWCTLNASYGVERIPTPRPPICKSEIEKLIRDSASSHENQVKCLKRLRQI 127

Query: 186 VASNSSAKKTVEDNGGLALISSLLGPFTSH-AVGSEAIGILVNLDLDGESRRNLVQPAK- 245
           V+ N++ K+ +E  G    +++++   + + ++  EA+ +L +L+      +NL+   K 
Sbjct: 128 VSENATNKRCLEAAGVPEFLANIVSNDSENGSLTDEALNLLYHLETSETVLKNLLNNKKD 187

Query: 246 ---ISLMVDMLNEGSSETKINCTGLIETLLEGDNCGVETVSSLSLLVGLLRLIKEKKHPN 305
              +  +  ++  G  E+++  T L++ +LE  +          +   +++++ ++    
Sbjct: 188 NNIVKSLTKIMQRGMYESRVYATLLLKNILEVADPMQSMTLKPEVFTEVVQILDDRISQK 247

Query: 306 GVLAGLRLLKALCSQEPVRNSVVSIGAIPQLIEIL--PNFDPDCL--ELALHVLDSLTSL 365
              A + +L  +C     R+  V  G I  +IE+L   +F  +    E+A+ VLD L   
Sbjct: 248 ATKAAMHILVNICPWGRNRHKAVEAGVISVIIELLMDESFTSERRGPEMAMVVLDLLCQC 307

Query: 366 SEGRVALKDCPNTIPNLVKLLLRVSESCTQLALSILWAVCKL-APEECASQAVEAGLAAK 425
           +EGR    +    I  + K +LRVS++ +  A+ +L +V +  A      + ++ G+ AK
Sbjct: 308 AEGRAEFLNHGAAIAVVCKKILRVSQTASDRAVRVLLSVGRFCATPALLHEMLQLGVVAK 367

Query: 426 L-LLIQCGCNPNLKQRSAELLKL 433
           L L++Q  C    K+++ ELLKL
Sbjct: 368 LCLVLQVSCGGKTKEKAKELLKL 390