Spg016191 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg016191 vs. NCBI nr
Match: XP_038899591.1 (triacylglycerol lipase OBL1-like [Benincasa hispida])

HSP 1 Score: 740.3 bits (1910), Expect = 1.0e-209
Identity = 358/481 (74.43%), Postives = 409/481 (85.03%), Query Frame = 0

Query: 6   EHAFSRNYLILKPENANILDLFLFLLPFGWRRRRFIDCSKGLEDSLRKFTDRLSIVISMV 65
           E  FS+NY+ILKPENAN+LDLFLFLLPFG R+R+FIDC  G ED  + F DR  I ISM+
Sbjct: 5   ESEFSKNYVILKPENANLLDLFLFLLPFGLRKRKFIDCPLGKEDPFKNFRDRWLIFISML 64

Query: 66  LQIILLAFATPLAKLDSFLLNLFNFISFNGGVLGLFFKILTVKKLLWPDKNSPEYMSLAA 125
           LQ IL   ATPLAKLDSFLL L NFISFNGGV G+  KIL  K  L  +++SPEY S+  
Sbjct: 65  LQFILAIIATPLAKLDSFLLKLLNFISFNGGVSGILSKILKGKSFLEVNEDSPEYTSVVG 124

Query: 126 FTDWRRDLDSSIEPSDTFRYYSALTVMAAKISYEFQPYVQSVVNHRWRMNLLGYFDFWND 185
           F DWRRDLDSSI+P DTFRYYSALTVMAAK+SYE +P+VQSVVN RW+M LLGYF+FWND
Sbjct: 125 FADWRRDLDSSIKPDDTFRYYSALTVMAAKVSYESKPFVQSVVNDRWKMKLLGYFNFWND 184

Query: 186 FQRKATTQAFMFETTATGPNVTVVAFRGTSPLDAYDWQVDADFSWYEIEGVGRMHSGFMK 245
           FQ KATTQAFMFE TA  PNV VVAFRGTS LD+YDWQVDADFSWYEIEGVGR+HSGFMK
Sbjct: 185 FQGKATTQAFMFENTAIDPNVIVVAFRGTSLLDSYDWQVDADFSWYEIEGVGRIHSGFMK 244

Query: 246 ALGLQKTTGWPKELPAGAQHDFAYYTLRQKLRDIARANHNARFIITGHSLGGALATVFVT 305
           ALGLQK TGWPKELP     +FAYYT+RQKLRDIA+ N  ARFIITGHSLGGALAT+FVT
Sbjct: 245 ALGLQKATGWPKELPKSQLQEFAYYTIRQKLRDIAKTNDKARFIITGHSLGGALATLFVT 304

Query: 306 ILALHDESSILEKLEGVYTYGQPRVGNRDFAKFMVDTVQKYSFKYYRYVYSSDLVPRVPS 365
           +L+LHDES+ILEKLEG+YTYGQPRVG++ FAKFMV+TVQKY FKY+RYVYSSDLVPR+PS
Sbjct: 305 VLSLHDESTILEKLEGIYTYGQPRVGDQQFAKFMVNTVQKYGFKYHRYVYSSDLVPRLPS 364

Query: 366 DGIFFKYRHFGKVVYFNSLYRGRILKEQPYKNYISLFWAIPKFLSAWWEVIRSWIIPFVR 425
           D I FKY+HFG+ V+FN+LYRGRI+KEQP KNY SL W IPK+LSAWWE+IRS IIP V+
Sbjct: 365 DAILFKYKHFGRCVFFNTLYRGRIVKEQPNKNYFSLVWVIPKYLSAWWELIRSLIIPLVK 424

Query: 426 GFDYYETLLMRGARVVGLLVPGVAAHFPVNYVNITRLGKLRMARQRQLEEPILEDDIEPD 485
           GFDYYE+LLM+G RVVGL +PG+AAHFP+NYVN TRLGKL +    ++E+PIL DDIE D
Sbjct: 425 GFDYYESLLMKGGRVVGLFIPGLAAHFPLNYVNSTRLGKLNV--PDKVEDPILGDDIEDD 483

Query: 486 Y 487
           +
Sbjct: 485 F 483

BLAST of Spg016191 vs. NCBI nr
Match: XP_023523371.1 (uncharacterized protein LOC111787588 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 731.5 bits (1887), Expect = 4.8e-207
Identity = 352/483 (72.88%), Postives = 414/483 (85.71%), Query Frame = 0

Query: 5   QEHAFSRNYLILKPENANILDLFLFLLPFGWRRRRFIDCSKGLEDSLRKFTDRLSIVISM 64
           +E  FS+NY+ILKP+NA +LDLFLFLLPFG R+ +FIDC KG EDS   FTDR SI IS+
Sbjct: 4   KECEFSKNYMILKPDNAKLLDLFLFLLPFGLRKTKFIDCPKGYEDSFNNFTDRWSIFISV 63

Query: 65  VLQIILLAFATPLAKLDSFLLNLFNFISFNGGVLGLFFKILTVKKLLWPDKNSPEYMSLA 124
           +LQIIL A ATPLAKLDSFL+NLFNFISFNGG+LGL  KIL  K L+ P+++S EY S+ 
Sbjct: 64  LLQIILAAAATPLAKLDSFLVNLFNFISFNGGILGLLSKILKGKGLVKPNEDSSEYTSVL 123

Query: 125 AFTDWRRDLDSSIEPSDTFRYYSALTVMAAKISYEFQPYVQSVVNHRWRMNLLGYFDFWN 184
            F DWR DLDSSI   DTFRYY  LT+MAAKI+YE +P+VQSVVN RW+M LLG+F+FWN
Sbjct: 124 GFADWRTDLDSSIPLHDTFRYYGGLTIMAAKIAYESKPFVQSVVNDRWKMKLLGFFNFWN 183

Query: 185 DFQRKATTQAFMFETTAT-GPNVTVVAFRGTSPLDAYDWQVDADFSWYEIEGVGRMHSGF 244
           DFQ KATTQAFMF+ TAT  PN+ V+AFRGTSPLD YDWQVDADFSWY+IEGVGR+HSGF
Sbjct: 184 DFQGKATTQAFMFQNTATDDPNIIVIAFRGTSPLDTYDWQVDADFSWYDIEGVGRIHSGF 243

Query: 245 MKALGLQKTTGWPKELPAGAQHDFAYYTLRQKLRDIARANHNARFIITGHSLGGALATVF 304
           MKALGLQK TGWPKEL    QHDFAYYTLRQKLRDIA+AN  ARFIITGHSLGGALAT+F
Sbjct: 244 MKALGLQKATGWPKEL-TKPQHDFAYYTLRQKLRDIAKANAKARFIITGHSLGGALATLF 303

Query: 305 VTILALHDESSILEKLEGVYTYGQPRVGNRDFAKFMVDTVQKYSFKYYRYVYSSDLVPRV 364
           VT+L++HDE+++LEKL+GVYTYGQPRVG+R FA+FMV++VQKY FKYYRYVYSSDLVPR+
Sbjct: 304 VTLLSMHDETAVLEKLQGVYTYGQPRVGDRRFAEFMVNSVQKYGFKYYRYVYSSDLVPRI 363

Query: 365 PSDGIFFKYRHFGKVVYFNSLYRGRILKEQPYKNYISLFWAIPKFLSAWWEVIRSWIIPF 424
           PSDG+ FKY+HF + +YFNSLY+GRI+KEQP KNY SL W IPK+LSAWWE++RS+I P 
Sbjct: 364 PSDGVIFKYKHFARSIYFNSLYKGRIVKEQPNKNYFSLLWVIPKYLSAWWELVRSFITPL 423

Query: 425 VRGFDYYETLLMRGARVVGLLVPGVAAHFPVNYVNITRLGKLRMARQRQLEEPILEDDIE 484
           V GFDYYE+LLM  AR+VGL++PG+AAHFP+NYVN  RLGKL+     ++E+PILEDDIE
Sbjct: 424 VWGFDYYESLLMSAARIVGLIIPGLAAHFPLNYVNSIRLGKLK--ELDEVEDPILEDDIE 483

Query: 485 PDY 487
            DY
Sbjct: 484 GDY 483

BLAST of Spg016191 vs. NCBI nr
Match: XP_022940183.1 (uncharacterized protein LOC111445886 [Cucurbita moschata])

HSP 1 Score: 724.5 bits (1869), Expect = 5.9e-205
Identity = 349/483 (72.26%), Postives = 413/483 (85.51%), Query Frame = 0

Query: 5   QEHAFSRNYLILKPENANILDLFLFLLPFGWRRRRFIDCSKGLEDSLRKFTDRLSIVISM 64
           +E  FS+NY+ILKP +A +LDLFLFLLPFG R+ +FIDC +G EDS   FTDR SI IS+
Sbjct: 4   KECEFSKNYMILKPNDAKLLDLFLFLLPFGLRKTKFIDCPEGNEDSYNNFTDRWSIFISV 63

Query: 65  VLQIILLAFATPLAKLDSFLLNLFNFISFNGGVLGLFFKILTVKKLLWPDKNSPEYMSLA 124
           +LQIIL A ATPLAKLDSFL+NLFNFISFNGG+LGL  KIL  K L+ P+++S +Y S+ 
Sbjct: 64  LLQIILAAAATPLAKLDSFLVNLFNFISFNGGILGLLAKILKGKGLVKPNEDSSDYTSVV 123

Query: 125 AFTDWRRDLDSSIEPSDTFRYYSALTVMAAKISYEFQPYVQSVVNHRWRMNLLGYFDFWN 184
            F DWR DLDSSI   DTFRYY  LT+MAAKI+YE +P+VQSVVN RW+M LLG+F+FWN
Sbjct: 124 GFADWRTDLDSSIPLHDTFRYYGGLTIMAAKIAYESKPFVQSVVNDRWKMKLLGFFNFWN 183

Query: 185 DFQRKATTQAFMFETTAT-GPNVTVVAFRGTSPLDAYDWQVDADFSWYEIEGVGRMHSGF 244
           DFQ KATTQAFMF+ TAT  PN+ V+AFRGTSPLD YDWQVDADFSWY+IEGVGR+HSGF
Sbjct: 184 DFQGKATTQAFMFQNTATDDPNIIVIAFRGTSPLDTYDWQVDADFSWYDIEGVGRIHSGF 243

Query: 245 MKALGLQKTTGWPKELPAGAQHDFAYYTLRQKLRDIARANHNARFIITGHSLGGALATVF 304
           MKALGLQK TGWPKEL    QHDFAYYTLRQKLRDIA+AN  ARFIITGHSLGGALAT+F
Sbjct: 244 MKALGLQKATGWPKEL-TKPQHDFAYYTLRQKLRDIAKANAKARFIITGHSLGGALATLF 303

Query: 305 VTILALHDESSILEKLEGVYTYGQPRVGNRDFAKFMVDTVQKYSFKYYRYVYSSDLVPRV 364
           VT+L+LHDE+ ILEKL+GVYTYGQPRVG+R FA+FMV++VQ+Y FKYYRYVYSSDLVPR+
Sbjct: 304 VTLLSLHDETPILEKLQGVYTYGQPRVGDRRFAEFMVNSVQQYGFKYYRYVYSSDLVPRI 363

Query: 365 PSDGIFFKYRHFGKVVYFNSLYRGRILKEQPYKNYISLFWAIPKFLSAWWEVIRSWIIPF 424
           PSDG+ FKY+HFG+ +YFN+LY+GRI+K QP KNY SL W IPK+LSAWWE++RS+I P 
Sbjct: 364 PSDGVIFKYKHFGRSIYFNTLYKGRIVKGQPNKNYFSLLWVIPKYLSAWWELVRSFITPL 423

Query: 425 VRGFDYYETLLMRGARVVGLLVPGVAAHFPVNYVNITRLGKLRMARQRQLEEPILEDDIE 484
           V GFDYYE+LLM  AR++GL++PG+AAHFP+NYVN TRLGKL+     ++E+PILEDDIE
Sbjct: 424 VWGFDYYESLLMSAARIIGLIIPGLAAHFPLNYVNSTRLGKLK--APDEVEDPILEDDIE 483

Query: 485 PDY 487
            DY
Sbjct: 484 GDY 483

BLAST of Spg016191 vs. NCBI nr
Match: XP_011654113.1 (uncharacterized protein LOC101203640 [Cucumis sativus] >KGN55199.1 hypothetical protein Csa_012910 [Cucumis sativus])

HSP 1 Score: 723.0 bits (1865), Expect = 1.7e-204
Identity = 344/481 (71.52%), Postives = 406/481 (84.41%), Query Frame = 0

Query: 6   EHAFSRNYLILKPENANILDLFLFLLPFGWRRRRFIDCSKGLEDSLRKFTDRLSIVISMV 65
           E+ FS+NY+ILKPENAN+LDLFLFLLPFG R+++FIDC  G ED    FTDR  IV+SM+
Sbjct: 5   ENEFSKNYVILKPENANLLDLFLFLLPFGLRKKKFIDCPYGKEDYFTNFTDRWLIVVSML 64

Query: 66  LQIILLAFATPLAKLDSFLLNLFNFISFNGGVLGLFFKILTVKKLLWPDKNSPEYMSLAA 125
           LQ IL   ATPLAKLD+FLL LFNFISFNGG LG+  KIL  K  +  +++S EY S+  
Sbjct: 65  LQFILSLIATPLAKLDAFLLKLFNFISFNGGFLGILSKILRGKGFVKVNEDSAEYTSVVG 124

Query: 126 FTDWRRDLDSSIEPSDTFRYYSALTVMAAKISYEFQPYVQSVVNHRWRMNLLGYFDFWND 185
           F DWRRDLDSSI+P  +FRYYSALT MA KISYE +P+VQSVVN RW+M LLGYF+FWND
Sbjct: 125 FADWRRDLDSSIKPEQSFRYYSALTAMATKISYESKPFVQSVVNDRWKMKLLGYFNFWND 184

Query: 186 FQRKATTQAFMFETTATGPNVTVVAFRGTSPLDAYDWQVDADFSWYEIEGVGRMHSGFMK 245
           FQ + TTQAFMF+ TA  PNVT+VAFRGTSPLDAYDWQVD DFSWYEIE VG +HSGFMK
Sbjct: 185 FQGRTTTQAFMFKNTAIDPNVTIVAFRGTSPLDAYDWQVDFDFSWYEIEDVGLIHSGFMK 244

Query: 246 ALGLQKTTGWPKELPAGAQHDFAYYTLRQKLRDIARANHNARFIITGHSLGGALATVFVT 305
           ALGLQK TGWPKELP    H+FAYYTLR++LRDIA+AN  ARFI TGHSLGGALAT+FVT
Sbjct: 245 ALGLQKATGWPKELPKTQTHEFAYYTLRKQLRDIAKANEKARFIFTGHSLGGALATLFVT 304

Query: 306 ILALHDESSILEKLEGVYTYGQPRVGNRDFAKFMVDTVQKYSFKYYRYVYSSDLVPRVPS 365
           +L LHDES+ILEKL+ VYTYGQPRVG++ FAKFM++ +QKY FKY+RYVYS DLVPRVPS
Sbjct: 305 VLCLHDESTILEKLDSVYTYGQPRVGDQQFAKFMLNCIQKYGFKYHRYVYSYDLVPRVPS 364

Query: 366 DGIFFKYRHFGKVVYFNSLYRGRILKEQPYKNYISLFWAIPKFLSAWWEVIRSWIIPFVR 425
           D + FKY+HFG+ VYFNSLY+GRI+KEQP KNY SL W IPK+LSAWWE+IRS+IIPFV+
Sbjct: 365 DAVLFKYKHFGRCVYFNSLYKGRIVKEQPNKNYFSLLWVIPKYLSAWWELIRSFIIPFVK 424

Query: 426 GFDYYETLLMRGARVVGLLVPGVAAHFPVNYVNITRLGKLRMARQRQLEEPILEDDIEPD 485
           GFDYYE+LLM+GAR+VGL++PG+ AHFP+NYVN+TRLGKL +    ++E+PIL  +IE D
Sbjct: 425 GFDYYESLLMKGARLVGLVIPGLTAHFPLNYVNVTRLGKLNV--PDEVEDPILRYEIEDD 483

Query: 486 Y 487
           Y
Sbjct: 485 Y 483

BLAST of Spg016191 vs. NCBI nr
Match: XP_008452563.1 (PREDICTED: uncharacterized protein LOC103493549 [Cucumis melo])

HSP 1 Score: 719.9 bits (1857), Expect = 1.4e-203
Identity = 344/481 (71.52%), Postives = 405/481 (84.20%), Query Frame = 0

Query: 6   EHAFSRNYLILKPENANILDLFLFLLPFGWRRRRFIDCSKGLEDSLRKFTDRLSIVISMV 65
           E+ FS+NY+ILKPENAN+LDLFLFLLPFG R+++FIDC  G ED    F DR  I +SM+
Sbjct: 5   ENEFSKNYVILKPENANLLDLFLFLLPFGLRKKKFIDCPYGKEDYFTNFADRWLIFVSML 64

Query: 66  LQIILLAFATPLAKLDSFLLNLFNFISFNGGVLGLFFKILTVKKLLWPDKNSPEYMSLAA 125
           LQ IL   ATPLAKLD+FLL LFNFISFNGG+LG+  KIL  K  +   ++S EY S+  
Sbjct: 65  LQFILALIATPLAKLDAFLLKLFNFISFNGGLLGILSKILRGKGFVKVKEDSSEYTSVVG 124

Query: 126 FTDWRRDLDSSIEPSDTFRYYSALTVMAAKISYEFQPYVQSVVNHRWRMNLLGYFDFWND 185
           F DWRRDLDSSI+   +FRYYSALTVMA KISYE +P+VQSVVN RW+M LLGYF+FWND
Sbjct: 125 FADWRRDLDSSIKSEKSFRYYSALTVMATKISYESKPFVQSVVNDRWKMKLLGYFNFWND 184

Query: 186 FQRKATTQAFMFETTATGPNVTVVAFRGTSPLDAYDWQVDADFSWYEIEGVGRMHSGFMK 245
           FQ KATTQAF FE TA  PNV +VAFRGTSPLDAYDWQVD D SWYEIEGVGR+HSGFMK
Sbjct: 185 FQGKATTQAFTFENTAIDPNVVIVAFRGTSPLDAYDWQVDVDLSWYEIEGVGRIHSGFMK 244

Query: 246 ALGLQKTTGWPKELPAGAQHDFAYYTLRQKLRDIARANHNARFIITGHSLGGALATVFVT 305
           ALGLQK TGWPKELP    H+FAYYTLR++LRDIA+AN  ARFI TGHSLGGALAT+FVT
Sbjct: 245 ALGLQKATGWPKELPKTQTHEFAYYTLRKQLRDIAKANEKARFIFTGHSLGGALATLFVT 304

Query: 306 ILALHDESSILEKLEGVYTYGQPRVGNRDFAKFMVDTVQKYSFKYYRYVYSSDLVPRVPS 365
           +L+LHDES+ILEKL+ VYTYGQPRVG++ FAKFMV+ VQKY FKY+RYVYS DLVPRVPS
Sbjct: 305 VLSLHDESTILEKLDSVYTYGQPRVGDQQFAKFMVNCVQKYGFKYHRYVYSYDLVPRVPS 364

Query: 366 DGIFFKYRHFGKVVYFNSLYRGRILKEQPYKNYISLFWAIPKFLSAWWEVIRSWIIPFVR 425
           D + FKY+HFG+ VYFNSLY+GRI+KEQP +NY SL W IPK+LSAWWE+IRS+IIPFV+
Sbjct: 365 DAVLFKYKHFGRCVYFNSLYKGRIVKEQPNRNYFSLLWVIPKYLSAWWELIRSFIIPFVK 424

Query: 426 GFDYYETLLMRGARVVGLLVPGVAAHFPVNYVNITRLGKLRMARQRQLEEPILEDDIEPD 485
           GFDYYE+LLM+GAR+VGLL+PG++AHFP+NYVN+TRLGKL +    ++E+P L  +IE D
Sbjct: 425 GFDYYESLLMKGARLVGLLIPGLSAHFPLNYVNVTRLGKLNV--PDEVEDPFLGYEIEDD 483

Query: 486 Y 487
           +
Sbjct: 485 F 483

BLAST of Spg016191 vs. ExPASy Swiss-Prot
Match: A0A1S3ZP85 (Triacylglycerol lipase OBL1 OS=Nicotiana tabacum OX=4097 GN=OBL1 PE=1 SV=1)

HSP 1 Score: 293.1 bits (749), Expect = 5.7e-78
Identity = 187/549 (34.06%), Postives = 283/549 (51.55%), Query Frame = 0

Query: 13  YLILKPENANILDLFLFLLPFGWRRR----RFIDCSKG---LEDSLRKFTD--------- 72
           +LI+  EN  I DL  F +   W  +    +F+  S G   LE+ L +  D         
Sbjct: 24  FLIVSHENGGIWDLVRFGV---WGNKESGDKFLHYSAGGGLLEEHLVRSDDSGGGDDRGG 83

Query: 73  -----RLSIVISMVLQIILLAFATPL---AKLDSFLLNLFNFISFNGGVLGLFFKILTVK 132
                R  I +S++++ ++  F  P+     L  F LNLF   S NG  LGL + IL   
Sbjct: 84  EVPDHRWVIFVSIIVRKLIAIFGKPMEWTGYLVEFFLNLF---SLNGNFLGLLYNILH-G 143

Query: 133 KLLWPDKNSPEYMSLAAFTDWRRDLDSS------IEPSDTF-------RYYSALTVMAAK 192
           K++ P + S  ++S     D R +L  S      I   D +       R    L +MA+K
Sbjct: 144 KVVMPHRGSETFISAIGHLDGRINLYKSETLTKEIGEPDFWQKIGIGHRDLMDLCMMASK 203

Query: 193 ISYEFQPYVQSVVNHRWRMNLLGYFDFWNDFQRKATTQAFMFETTATGPNVTVVAFRGTS 252
           ++YE +  V++VVN  W+M+ + +++ WNDF+++ +TQ F+        N+ +V+FRGT 
Sbjct: 204 LAYENEKVVRNVVNLHWKMHFVDFYNCWNDFEKEMSTQVFLLCDKPKDANLILVSFRGTE 263

Query: 253 PLDAYDWQVDADFSWYEIEGVGRMHSGFMKALGLQKTTG--------------------- 312
           P DA DW  D D+SWYEI  +G++H GF++ALGL   T                      
Sbjct: 264 PFDADDWITDFDYSWYEIPKLGKVHMGFLEALGLGNRTNASTFHEQLFVNNLKFANLENV 323

Query: 313 ---WPKELPAGAQHDF----------------------------------AYYTLRQKLR 372
               P    + +   F                                  AYY +R KL+
Sbjct: 324 HATIPPSESSKSSTSFSDSDAHTGSDLSSDSERPTDTRKKKFRLEIPERTAYYVVRSKLK 383

Query: 373 DIARANHNARFIITGHSLGGALATVFVTILALHDESSILEKLEGVYTYGQPRVGNRDFAK 432
            + + + NA+F++TGHSLGGALA +F  +L LH+E  ++E+L G+YTYGQPRVGNR   +
Sbjct: 384 RLLKEHKNAKFVVTGHSLGGALAILFPAVLVLHEEVDVMERLLGIYTYGQPRVGNRQLGR 443

Query: 433 FMVDTVQKYSFKYYRYVYSSDLVPRVPSDGIFFKYRHFGKVVYFNSLYRGRILKEQPYKN 467
           FM   ++    KY+R VY +DLVPR+P D   F ++HFG   Y+NSLY  + + E+P  N
Sbjct: 444 FMEAHLEHPVPKYFRVVYCNDLVPRLPYDNKTFLFKHFGICQYYNSLYIEQNINEEPNPN 503

BLAST of Spg016191 vs. ExPASy Swiss-Prot
Match: F4JFU8 (Triacylglycerol lipase OBL1 OS=Arabidopsis thaliana OX=3702 GN=OBL1 PE=1 SV=1)

HSP 1 Score: 283.5 bits (724), Expect = 4.5e-75
Identity = 170/503 (33.80%), Postives = 273/503 (54.27%), Query Frame = 0

Query: 2   GSDQEHAFSRNYLILKPENANILDLFLF-LLPFGWRRRRFIDCSKGLEDSLRKFTD---- 61
           GS        NYLI++P     +DLF + +      + +F++     E S     +    
Sbjct: 8   GSGSNPGQVSNYLIVRPHRGGYIDLFRYGVRDDQTSKAKFLEMPDNREWSTITIDEEAED 67

Query: 62  -RLSIVISMVLQIILLAFATPLAKLDSFLLNLF-NFISFNGGVLGLFFKILTVKKLLWPD 121
            R  IV+S++++ I+    TP+ +   F+++ F N  S NGG  GL  +++  K ++ P+
Sbjct: 68  HRWVIVVSILVRKIIRLLRTPM-EFTGFVVDFFLNLFSANGGFFGLLLRLIQAKVVI-PE 127

Query: 122 KNSPEYMSLAAFTDWRRDLDSS------IEPSDTF-----------RYYSALTVMAAKIS 181
           + S  ++S     D R  L         +E  D+            R    L VMA+K++
Sbjct: 128 RGSVTFVSTIGQLDGRISLYKEWNFVEHLEGIDSVDSGRVKIELGSRGLMDLCVMASKLA 187

Query: 182 YEFQPYVQSVVNHRWRMNLLGYFDFWNDFQRKATTQAFMFETTATGPNVTVVAFRGTSPL 241
           YE    V++VV+  W+MNL+ + D WND+Q++ +TQ F+F       N+ V++FRGT P 
Sbjct: 188 YENAKVVENVVDLHWKMNLVEFLDCWNDYQKQMSTQVFVFTDKQKDANLIVISFRGTEPF 247

Query: 242 DAYDWQVDADFSWYEIEGVGRMHSGFMKALGL---QKTTGWPKELPAGAQHD-------- 301
           DA DW  D D+SWYE+  VG++H GF++A+GL     TT +   L      +        
Sbjct: 248 DADDWGTDFDYSWYEVPNVGKLHMGFLEAMGLGNRDDTTTFHYNLFEQTSSEEENSKKNL 307

Query: 302 ------FAYYTLRQKLRDIARANHNARFIITGHSLGGALATVFVTILALHDESSILEKLE 361
                  AYY +R  L+ +   + NARF++TGHSLGGALA +F T+L L++E+ I+++L 
Sbjct: 308 LDMVERSAYYAVRVILKRLLSEHENARFVVTGHSLGGALAILFPTLLVLNEETEIMKRLL 367

Query: 362 GVYTYGQPRVGNRDFAKFMVDTVQKYSFKYYRYVYSSDLVPRVPSDGIFFKYRHFGKVVY 421
           GVYT+GQPR+GNR+   FM   + +   +Y+R VY +D+VPR+P D   F Y+HFG  ++
Sbjct: 368 GVYTFGQPRIGNREVGLFMKAQLNQPVDRYFRVVYCNDIVPRLPYDDKTFLYKHFGLCLF 427

Query: 422 FNSLYRGRILKEQPYKNYISLFWAIPKFLSAWWEVIRSWIIPFVRGFDYYETLLMRGARV 464
           ++S Y     +++P  N   L + I   + A WE++R   + +  G DY E       R+
Sbjct: 428 YDSFYNETKAEDEPDPNPYGLRYKILGHVIAVWELVRGLTMGYTHGPDYKEGWFRILFRL 487

BLAST of Spg016191 vs. ExPASy Swiss-Prot
Match: Q5VKJ7 (Triacylglycerol lipase OBL1 OS=Ricinus communis OX=3988 GN=OBL1 PE=1 SV=1)

HSP 1 Score: 265.8 bits (678), Expect = 9.8e-70
Identity = 169/508 (33.27%), Postives = 257/508 (50.59%), Query Frame = 0

Query: 10  SRNYLILKPENANILDLF--LFLLPFGWRRRRFIDCSKGLEDSLRKFTDR--LSIVISMV 69
           S ++LI+ P+    LDLF  + L   G    +F   S     +   +  R  +S+ I  +
Sbjct: 9   STSHLIVSPDEGTFLDLFKHIVLSDLG-SGAKFFRASDQRVPATAAYYSRWPVSVFICKI 68

Query: 70  LQIILLAFATPLAKLDSFLLNLFNFISFNGGVLGLFFKILTVKKLLWPDKNSPEYMSLAA 129
           LQ+    F  P A L      L NF   N G LG+   I  + +L  P +   +++S   
Sbjct: 69  LQL----FQMPAAMLGHLTDFLLNFYYQNHGFLGILRNIFLI-RLKIPKRGEADFISTIG 128

Query: 130 FTDWRRDL--------------------------DSSIEPSDTFRYYSALTVMAAKISYE 189
           + D R DL                          +S I+ +   R    L +MA+K++YE
Sbjct: 129 YLDSRMDLHGTPMVSHQADEVISNADNPSLKEGHNSKIKGALGNRSLMDLCIMASKLAYE 188

Query: 190 FQPYVQSVVNHRWRMNLLGYFDFWNDFQRKATTQAFMFETTATGPNVTVVAFRGTSPLDA 249
               V+ VV   W+M+ +  +   N FQ    T AF+F       N+ V++FRGT P   
Sbjct: 189 NTKVVERVVAEHWKMHFVADYGGMNYFQDARNTHAFIFCDKPKDANLIVISFRGTGPFSI 248

Query: 250 YDWQVDADFSWYEIEGVGRMHSGFMKALGL--------------QKTTGWPKELPAGAQ- 309
            +W  D DFS   +   G +H GF++A+GL               K+ G   EL   ++ 
Sbjct: 249 PNWCTDFDFSLVGLGDAGSVHVGFLEAMGLGHRNSISSFETSINTKSPGSITELRKESEM 308

Query: 310 --------HDFAYYTLRQKLRDIARANHNARFIITGHSLGGALATVFVTILALHDESSIL 369
                   +D  Y+     L+ + + + NA+F++TGHSLGGALA +F  IL +  E+ +L
Sbjct: 309 APDHLVWAYDGVYFLAASTLKGLLKDHKNAKFVVTGHSLGGALAILFTCILEIQQETEVL 368

Query: 370 EKLEGVYTYGQPRVGNRDFAKFMVDTVQKYSFKYYRYVYSSDLVPRVPSDGIFFKYRHFG 429
           ++L  VYT+GQPR+GN +   FM + +     +Y+R VY +D+VPRVP D +FF + HFG
Sbjct: 369 DRLLNVYTFGQPRIGNYNLGYFMQNRLNFPERRYFRVVYCNDMVPRVPFDDVFFTFEHFG 428

Query: 430 KVVYFNSLYRGRILKEQPYKNYISLFWAIPKFLSAWWEVIRSWIIPFVRGFDYYETLLMR 465
             +Y++S + G   KE+P +N   +  AI   ++AWWE+ RS+I+  V G +Y ET   R
Sbjct: 429 TCIYYDSRFFGYFTKEEPSRNPFGIENAISAHITAWWELWRSFILNHVYGAEYKETWESR 488

BLAST of Spg016191 vs. ExPASy Swiss-Prot
Match: O59952 (Lipase OS=Thermomyces lanuginosus OX=5541 GN=LIP PE=1 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 7.1e-12
Identity = 57/160 (35.62%), Postives = 81/160 (50.62%), Query Frame = 0

Query: 208 VVAFRGTSPLDAYDWQVDADFSWYEIEGV---GRMHSGFMKALGLQKTTGWPKELPAGAQ 267
           V++FRG+  ++  +W  + +F   EI  +    R H GF        T+ W         
Sbjct: 99  VLSFRGSRSIE--NWIGNLNFDLKEINDICSGCRGHDGF--------TSSWRSVAD---- 158

Query: 268 HDFAYYTLRQKLRDIARANHNARFIITGHSLGGALATVFVTILALHDESSILEKLEGVYT 327
                 TLRQK+ D  R + + R + TGHSLGGALATV    L  +           V++
Sbjct: 159 ------TLRQKVEDAVREHPDYRVVFTGHSLGGALATVAGADLRGNGYDI------DVFS 218

Query: 328 YGQPRVGNRDFAKFMVDTVQKYSFKYYRYVYSSDLVPRVP 365
           YG PRVGNR FA+F+  TVQ      YR  +++D+VPR+P
Sbjct: 219 YGAPRVGNRAFAEFL--TVQT-GGTLYRITHTNDIVPRLP 229

BLAST of Spg016191 vs. ExPASy Swiss-Prot
Match: P19515 (Lipase OS=Rhizomucor miehei OX=4839 PE=1 SV=2)

HSP 1 Score: 58.5 bits (140), Expect = 2.4e-07
Identity = 52/172 (30.23%), Postives = 76/172 (44.19%), Query Frame = 0

Query: 209 VAFRGTSPLDAYDWQVDADF---SWYEIEGVGRMHSGFMKALGLQKTTGWPKELPAGAQH 268
           + FRG+S +   +W  D  F   S+  + G  ++H GF+ + G  +      EL A    
Sbjct: 171 IVFRGSSSI--RNWIADLTFVPVSYPPVSGT-KVHKGFLDSYGEVQ-----NELVATVLD 230

Query: 269 DFAYYTLRQKLRDIARANHNARFIITGHSLGGALATVFVTILALHDESSILEKLEGVYTY 328
            F  Y              + +  +TGHSLGGA A +    L   +E      L  +YT 
Sbjct: 231 QFKQYP-------------SYKVAVTGHSLGGATALLCALDLYQREEGLSSSNL-FLYTQ 290

Query: 329 GQPRVGNRDFAKFMVDTVQKYSFKYYRYVYSSDLVPRVPSDGIFFKYRHFGK 378
           GQPRVG+  FA ++V T       Y R V   D+VP +P     F + H G+
Sbjct: 291 GQPRVGDPAFANYVVST----GIPYRRTVNERDIVPHLPPAA--FGFLHAGE 314

BLAST of Spg016191 vs. ExPASy TrEMBL
Match: A0A6J1FNK8 (uncharacterized protein LOC111445886 OS=Cucurbita moschata OX=3662 GN=LOC111445886 PE=4 SV=1)

HSP 1 Score: 724.5 bits (1869), Expect = 2.8e-205
Identity = 349/483 (72.26%), Postives = 413/483 (85.51%), Query Frame = 0

Query: 5   QEHAFSRNYLILKPENANILDLFLFLLPFGWRRRRFIDCSKGLEDSLRKFTDRLSIVISM 64
           +E  FS+NY+ILKP +A +LDLFLFLLPFG R+ +FIDC +G EDS   FTDR SI IS+
Sbjct: 4   KECEFSKNYMILKPNDAKLLDLFLFLLPFGLRKTKFIDCPEGNEDSYNNFTDRWSIFISV 63

Query: 65  VLQIILLAFATPLAKLDSFLLNLFNFISFNGGVLGLFFKILTVKKLLWPDKNSPEYMSLA 124
           +LQIIL A ATPLAKLDSFL+NLFNFISFNGG+LGL  KIL  K L+ P+++S +Y S+ 
Sbjct: 64  LLQIILAAAATPLAKLDSFLVNLFNFISFNGGILGLLAKILKGKGLVKPNEDSSDYTSVV 123

Query: 125 AFTDWRRDLDSSIEPSDTFRYYSALTVMAAKISYEFQPYVQSVVNHRWRMNLLGYFDFWN 184
            F DWR DLDSSI   DTFRYY  LT+MAAKI+YE +P+VQSVVN RW+M LLG+F+FWN
Sbjct: 124 GFADWRTDLDSSIPLHDTFRYYGGLTIMAAKIAYESKPFVQSVVNDRWKMKLLGFFNFWN 183

Query: 185 DFQRKATTQAFMFETTAT-GPNVTVVAFRGTSPLDAYDWQVDADFSWYEIEGVGRMHSGF 244
           DFQ KATTQAFMF+ TAT  PN+ V+AFRGTSPLD YDWQVDADFSWY+IEGVGR+HSGF
Sbjct: 184 DFQGKATTQAFMFQNTATDDPNIIVIAFRGTSPLDTYDWQVDADFSWYDIEGVGRIHSGF 243

Query: 245 MKALGLQKTTGWPKELPAGAQHDFAYYTLRQKLRDIARANHNARFIITGHSLGGALATVF 304
           MKALGLQK TGWPKEL    QHDFAYYTLRQKLRDIA+AN  ARFIITGHSLGGALAT+F
Sbjct: 244 MKALGLQKATGWPKEL-TKPQHDFAYYTLRQKLRDIAKANAKARFIITGHSLGGALATLF 303

Query: 305 VTILALHDESSILEKLEGVYTYGQPRVGNRDFAKFMVDTVQKYSFKYYRYVYSSDLVPRV 364
           VT+L+LHDE+ ILEKL+GVYTYGQPRVG+R FA+FMV++VQ+Y FKYYRYVYSSDLVPR+
Sbjct: 304 VTLLSLHDETPILEKLQGVYTYGQPRVGDRRFAEFMVNSVQQYGFKYYRYVYSSDLVPRI 363

Query: 365 PSDGIFFKYRHFGKVVYFNSLYRGRILKEQPYKNYISLFWAIPKFLSAWWEVIRSWIIPF 424
           PSDG+ FKY+HFG+ +YFN+LY+GRI+K QP KNY SL W IPK+LSAWWE++RS+I P 
Sbjct: 364 PSDGVIFKYKHFGRSIYFNTLYKGRIVKGQPNKNYFSLLWVIPKYLSAWWELVRSFITPL 423

Query: 425 VRGFDYYETLLMRGARVVGLLVPGVAAHFPVNYVNITRLGKLRMARQRQLEEPILEDDIE 484
           V GFDYYE+LLM  AR++GL++PG+AAHFP+NYVN TRLGKL+     ++E+PILEDDIE
Sbjct: 424 VWGFDYYESLLMSAARIIGLIIPGLAAHFPLNYVNSTRLGKLK--APDEVEDPILEDDIE 483

Query: 485 PDY 487
            DY
Sbjct: 484 GDY 483

BLAST of Spg016191 vs. ExPASy TrEMBL
Match: A0A0A0L2D5 (Lipase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G639870 PE=4 SV=1)

HSP 1 Score: 723.0 bits (1865), Expect = 8.3e-205
Identity = 344/481 (71.52%), Postives = 406/481 (84.41%), Query Frame = 0

Query: 6   EHAFSRNYLILKPENANILDLFLFLLPFGWRRRRFIDCSKGLEDSLRKFTDRLSIVISMV 65
           E+ FS+NY+ILKPENAN+LDLFLFLLPFG R+++FIDC  G ED    FTDR  IV+SM+
Sbjct: 5   ENEFSKNYVILKPENANLLDLFLFLLPFGLRKKKFIDCPYGKEDYFTNFTDRWLIVVSML 64

Query: 66  LQIILLAFATPLAKLDSFLLNLFNFISFNGGVLGLFFKILTVKKLLWPDKNSPEYMSLAA 125
           LQ IL   ATPLAKLD+FLL LFNFISFNGG LG+  KIL  K  +  +++S EY S+  
Sbjct: 65  LQFILSLIATPLAKLDAFLLKLFNFISFNGGFLGILSKILRGKGFVKVNEDSAEYTSVVG 124

Query: 126 FTDWRRDLDSSIEPSDTFRYYSALTVMAAKISYEFQPYVQSVVNHRWRMNLLGYFDFWND 185
           F DWRRDLDSSI+P  +FRYYSALT MA KISYE +P+VQSVVN RW+M LLGYF+FWND
Sbjct: 125 FADWRRDLDSSIKPEQSFRYYSALTAMATKISYESKPFVQSVVNDRWKMKLLGYFNFWND 184

Query: 186 FQRKATTQAFMFETTATGPNVTVVAFRGTSPLDAYDWQVDADFSWYEIEGVGRMHSGFMK 245
           FQ + TTQAFMF+ TA  PNVT+VAFRGTSPLDAYDWQVD DFSWYEIE VG +HSGFMK
Sbjct: 185 FQGRTTTQAFMFKNTAIDPNVTIVAFRGTSPLDAYDWQVDFDFSWYEIEDVGLIHSGFMK 244

Query: 246 ALGLQKTTGWPKELPAGAQHDFAYYTLRQKLRDIARANHNARFIITGHSLGGALATVFVT 305
           ALGLQK TGWPKELP    H+FAYYTLR++LRDIA+AN  ARFI TGHSLGGALAT+FVT
Sbjct: 245 ALGLQKATGWPKELPKTQTHEFAYYTLRKQLRDIAKANEKARFIFTGHSLGGALATLFVT 304

Query: 306 ILALHDESSILEKLEGVYTYGQPRVGNRDFAKFMVDTVQKYSFKYYRYVYSSDLVPRVPS 365
           +L LHDES+ILEKL+ VYTYGQPRVG++ FAKFM++ +QKY FKY+RYVYS DLVPRVPS
Sbjct: 305 VLCLHDESTILEKLDSVYTYGQPRVGDQQFAKFMLNCIQKYGFKYHRYVYSYDLVPRVPS 364

Query: 366 DGIFFKYRHFGKVVYFNSLYRGRILKEQPYKNYISLFWAIPKFLSAWWEVIRSWIIPFVR 425
           D + FKY+HFG+ VYFNSLY+GRI+KEQP KNY SL W IPK+LSAWWE+IRS+IIPFV+
Sbjct: 365 DAVLFKYKHFGRCVYFNSLYKGRIVKEQPNKNYFSLLWVIPKYLSAWWELIRSFIIPFVK 424

Query: 426 GFDYYETLLMRGARVVGLLVPGVAAHFPVNYVNITRLGKLRMARQRQLEEPILEDDIEPD 485
           GFDYYE+LLM+GAR+VGL++PG+ AHFP+NYVN+TRLGKL +    ++E+PIL  +IE D
Sbjct: 425 GFDYYESLLMKGARLVGLVIPGLTAHFPLNYVNVTRLGKLNV--PDEVEDPILRYEIEDD 483

Query: 486 Y 487
           Y
Sbjct: 485 Y 483

BLAST of Spg016191 vs. ExPASy TrEMBL
Match: A0A1S3BUY0 (uncharacterized protein LOC103493549 OS=Cucumis melo OX=3656 GN=LOC103493549 PE=4 SV=1)

HSP 1 Score: 719.9 bits (1857), Expect = 7.0e-204
Identity = 344/481 (71.52%), Postives = 405/481 (84.20%), Query Frame = 0

Query: 6   EHAFSRNYLILKPENANILDLFLFLLPFGWRRRRFIDCSKGLEDSLRKFTDRLSIVISMV 65
           E+ FS+NY+ILKPENAN+LDLFLFLLPFG R+++FIDC  G ED    F DR  I +SM+
Sbjct: 5   ENEFSKNYVILKPENANLLDLFLFLLPFGLRKKKFIDCPYGKEDYFTNFADRWLIFVSML 64

Query: 66  LQIILLAFATPLAKLDSFLLNLFNFISFNGGVLGLFFKILTVKKLLWPDKNSPEYMSLAA 125
           LQ IL   ATPLAKLD+FLL LFNFISFNGG+LG+  KIL  K  +   ++S EY S+  
Sbjct: 65  LQFILALIATPLAKLDAFLLKLFNFISFNGGLLGILSKILRGKGFVKVKEDSSEYTSVVG 124

Query: 126 FTDWRRDLDSSIEPSDTFRYYSALTVMAAKISYEFQPYVQSVVNHRWRMNLLGYFDFWND 185
           F DWRRDLDSSI+   +FRYYSALTVMA KISYE +P+VQSVVN RW+M LLGYF+FWND
Sbjct: 125 FADWRRDLDSSIKSEKSFRYYSALTVMATKISYESKPFVQSVVNDRWKMKLLGYFNFWND 184

Query: 186 FQRKATTQAFMFETTATGPNVTVVAFRGTSPLDAYDWQVDADFSWYEIEGVGRMHSGFMK 245
           FQ KATTQAF FE TA  PNV +VAFRGTSPLDAYDWQVD D SWYEIEGVGR+HSGFMK
Sbjct: 185 FQGKATTQAFTFENTAIDPNVVIVAFRGTSPLDAYDWQVDVDLSWYEIEGVGRIHSGFMK 244

Query: 246 ALGLQKTTGWPKELPAGAQHDFAYYTLRQKLRDIARANHNARFIITGHSLGGALATVFVT 305
           ALGLQK TGWPKELP    H+FAYYTLR++LRDIA+AN  ARFI TGHSLGGALAT+FVT
Sbjct: 245 ALGLQKATGWPKELPKTQTHEFAYYTLRKQLRDIAKANEKARFIFTGHSLGGALATLFVT 304

Query: 306 ILALHDESSILEKLEGVYTYGQPRVGNRDFAKFMVDTVQKYSFKYYRYVYSSDLVPRVPS 365
           +L+LHDES+ILEKL+ VYTYGQPRVG++ FAKFMV+ VQKY FKY+RYVYS DLVPRVPS
Sbjct: 305 VLSLHDESTILEKLDSVYTYGQPRVGDQQFAKFMVNCVQKYGFKYHRYVYSYDLVPRVPS 364

Query: 366 DGIFFKYRHFGKVVYFNSLYRGRILKEQPYKNYISLFWAIPKFLSAWWEVIRSWIIPFVR 425
           D + FKY+HFG+ VYFNSLY+GRI+KEQP +NY SL W IPK+LSAWWE+IRS+IIPFV+
Sbjct: 365 DAVLFKYKHFGRCVYFNSLYKGRIVKEQPNRNYFSLLWVIPKYLSAWWELIRSFIIPFVK 424

Query: 426 GFDYYETLLMRGARVVGLLVPGVAAHFPVNYVNITRLGKLRMARQRQLEEPILEDDIEPD 485
           GFDYYE+LLM+GAR+VGLL+PG++AHFP+NYVN+TRLGKL +    ++E+P L  +IE D
Sbjct: 425 GFDYYESLLMKGARLVGLLIPGLSAHFPLNYVNVTRLGKLNV--PDEVEDPFLGYEIEDD 483

Query: 486 Y 487
           +
Sbjct: 485 F 483

BLAST of Spg016191 vs. ExPASy TrEMBL
Match: A0A6J1J3Y9 (uncharacterized protein LOC111481121 OS=Cucurbita maxima OX=3661 GN=LOC111481121 PE=4 SV=1)

HSP 1 Score: 709.5 bits (1830), Expect = 9.4e-201
Identity = 342/479 (71.40%), Postives = 410/479 (85.59%), Query Frame = 0

Query: 9   FSRNYLILKPENANILDLFLFLLPFGWRRRRFIDCSKGLEDSLRKFTDRLSIVISMVLQI 68
           FS+NY+ILKP +A +LDLFLFLLPFG R+ +FIDC +G E S   FTDR  I IS++LQI
Sbjct: 8   FSKNYMILKPNDAKLLDLFLFLLPFGLRKTKFIDCPEGNEASYSNFTDRWFIFISILLQI 67

Query: 69  ILLAFATPLAKLDSFLLNLFNFISFNGGVLGLFFKILTVKKLLWPDKNSPEYMSLAAFTD 128
           IL A ATPL+KLDSFL+NLFNFISFNGG+LGL  KIL  K L+ P+++S +Y S+  F D
Sbjct: 68  ILAAAATPLSKLDSFLVNLFNFISFNGGILGLLSKILKGKGLVKPNEDSSDYTSVLGFAD 127

Query: 129 WRRDLDSSIEPSDTFRYYSALTVMAAKISYEFQPYVQSVVNHRWRMNLLGYFDFWNDFQR 188
           WR DLDSSI   DTFRYY  LT+MAAKI+YE +P+VQSVVN RW+M LLG+F+FWNDFQ 
Sbjct: 128 WRTDLDSSIPLHDTFRYYGGLTIMAAKIAYESKPFVQSVVNDRWKMKLLGFFNFWNDFQG 187

Query: 189 KATTQAFMFETTAT-GPNVTVVAFRGTSPLDAYDWQVDADFSWYEIEGVGRMHSGFMKAL 248
           KATTQAFMF+ TAT  PN+ V+AFRGTSPLD  DWQV+ADFSWY+IEG+GR+HSGFMKAL
Sbjct: 188 KATTQAFMFQNTATDDPNIIVIAFRGTSPLDTDDWQVNADFSWYDIEGLGRIHSGFMKAL 247

Query: 249 GLQKTTGWPKELPAGAQHDFAYYTLRQKLRDIARANHNARFIITGHSLGGALATVFVTIL 308
           GLQK TGWPKEL    QHDFAYY+LRQKLRDIA+AN  ARFIITGHSLGGALAT+FVT+L
Sbjct: 248 GLQKATGWPKEL-TKPQHDFAYYSLRQKLRDIAKANAKARFIITGHSLGGALATLFVTLL 307

Query: 309 ALHDESSILEKLEGVYTYGQPRVGNRDFAKFMVDTVQKYSFKYYRYVYSSDLVPRVPSDG 368
           +LHDE++ILEKL+GVYTYGQPRVG+R FA+FMV+TVQKY FKYYRYVYSSDLVPR+PSDG
Sbjct: 308 SLHDETTILEKLQGVYTYGQPRVGDRRFAEFMVNTVQKYGFKYYRYVYSSDLVPRIPSDG 367

Query: 369 IFFKYRHFGKVVYFNSLYRGRILKEQPYKNYISLFWAIPKFLSAWWEVIRSWIIPFVRGF 428
           + FKY+HFG+ +YFNSLY+GRI+KEQP KNY SL W IPK+LSAWWE++RS+I P + GF
Sbjct: 368 VIFKYKHFGRSIYFNSLYKGRIVKEQPNKNYFSLLWVIPKYLSAWWELVRSFITPLMWGF 427

Query: 429 DYYETLLMRGARVVGLLVPGVAAHFPVNYVNITRLGKLRMARQRQLEEPILEDDIEPDY 487
           D+YE+LLM  AR+VGL++PG+AAHFP+NYVN  RLGKL++    ++E+PILEDDIE +Y
Sbjct: 428 DHYESLLMSAARMVGLIIPGLAAHFPLNYVNSIRLGKLKV--PDEIEDPILEDDIEGEY 483

BLAST of Spg016191 vs. ExPASy TrEMBL
Match: A0A6J1CEI9 (uncharacterized protein LOC111010491 OS=Momordica charantia OX=3673 GN=LOC111010491 PE=4 SV=1)

HSP 1 Score: 699.9 bits (1805), Expect = 7.5e-198
Identity = 338/485 (69.69%), Postives = 407/485 (83.92%), Query Frame = 0

Query: 3   SDQEHAFSRNYLILKPENANILDLFLFLLPFGWRRR-RFIDCSKGLEDS-LRKFTDRLSI 62
           + +EH FS+NYLILKP+NANI+DLFLFLLPFG+R+R +FIDC +G EDS    FT+R  I
Sbjct: 2   ASKEHEFSKNYLILKPQNANIVDLFLFLLPFGFRKRTKFIDCPEGKEDSYTSSFTNRWLI 61

Query: 63  VISMVLQIILLAFATPLAKLDSFLLNLFNFISFNGGVLGLFFKILTVKKLLWPDKNSPEY 122
            IS+VLQIILLA ATPLAKLD+FLLN+FNFISFNGG+LGL  +IL  + L+ PD+NS EY
Sbjct: 62  FISIVLQIILLAIATPLAKLDAFLLNVFNFISFNGGILGLLSRILKGESLVKPDENSAEY 121

Query: 123 MSLAAFTDWRRDLDSSIEPSDTFRYYSALTVMAAKISYEFQPYVQSVVNHRWRMNLLGYF 182
            S+  F DWRRDLD SI+  + FRYY  LTVMAA++SYE +P+V SVVN RW+M LLG+F
Sbjct: 122 TSVVGFADWRRDLDKSIKAEEIFRYYGGLTVMAAQLSYESKPFVLSVVNDRWKMKLLGHF 181

Query: 183 DFWNDFQRKATTQAFMFETTATGPNVTVVAFRGTSPLDAYDWQVDADFSWYEIEGVGRMH 242
           +FWNDFQ KATTQAFMF+ TA  PNVTVVAFRGTSPLDAYDWQVDADFSWY+IEGVGR+H
Sbjct: 182 NFWNDFQGKATTQAFMFQNTAADPNVTVVAFRGTSPLDAYDWQVDADFSWYDIEGVGRIH 241

Query: 243 SGFMKALGLQKTTGWPKELPAGAQHDFAYYTLRQKLRDIARANHNARFIITGHSLGGALA 302
           SGFMKALGLQK TGWP+EL   ++H FAYYTLRQKLRDIA++N NA+FIITGHSLGGALA
Sbjct: 242 SGFMKALGLQKATGWPEEL-VKSKHHFAYYTLRQKLRDIAKSNDNAKFIITGHSLGGALA 301

Query: 303 TVFVTILALHDESSILEKLEGVYTYGQPRVGNRDFAKFMVDTVQKYSFKYYRYVYSSDLV 362
            +FVT+L+LH +S++LEKL+ VYTY QPRVG+R FA+FMV+TV+KY FKY+RYVYSSDLV
Sbjct: 302 ALFVTVLSLHRDSAVLEKLQAVYTYSQPRVGDRRFAEFMVNTVKKYGFKYHRYVYSSDLV 361

Query: 363 PRVPSDGIFFKYRHFGKVVYFNSLYRGRILKEQPYKNYISLFWAIPKFLSAWWEVIRSWI 422
           PRVP DG+ FKY+HFG+ +YFNSLY+GRI+KE+P KNY SL W +PK+L+AWWE+IRS+I
Sbjct: 362 PRVPFDGVIFKYKHFGRCIYFNSLYKGRIVKEEPNKNYFSLIWVMPKYLNAWWELIRSFI 421

Query: 423 IPFVRGFDYYETLLMRGARVVGLLVPGVAAHFPVNYVNITRLGKLRMARQRQLEEPILED 482
           +P V+GFDY+E+LLM+GARVVGL +PG+AAH P +YVN  RLGK     Q        ED
Sbjct: 422 VPLVKGFDYHESLLMKGARVVGLFIPGLAAHIPTDYVNSIRLGKFNPPPQE------FED 479

Query: 483 DIEPD 486
            IE D
Sbjct: 482 SIEAD 479

BLAST of Spg016191 vs. TAIR 10
Match: AT1G45201.1 (triacylglycerol lipase-like 1 )

HSP 1 Score: 416.0 bits (1068), Expect = 4.2e-116
Identity = 212/459 (46.19%), Postives = 298/459 (64.92%), Query Frame = 0

Query: 9   FSRNYLILKPENANILDLFLFLLPFGWRRRRFIDCSKG-LEDSLRKFTDRLSIVISMVLQ 68
           F  +Y ++ P  A+ LDL L L        RFID     L+   R F  R  + +++ LQ
Sbjct: 8   FCNSYFLVDPTKASFLDLLLLLFSSNLTSARFIDSPPDTLKGFRRSFASRWILALAIFLQ 67

Query: 69  IILLAFATPLAKLDSFLLNLFNFISFNGGVLGLFFKILTVKKLLWPDKNSPEYMSLAAFT 128
            +L+  + P A +   L    N ++ NGG   L   +++  KL+ PDK+S  Y S    +
Sbjct: 68  KVLMLLSKPFAFIGQKLTYWLNLLTANGGFFNLILNLMS-GKLVKPDKSSATYTSFIGCS 127

Query: 129 DWRRDLDSSIEPSDTFRYYSALTVMAAKISYEFQPYVQSVVNHRWRMNLLGYFDFWNDFQ 188
           D R +LD  I    +  Y S L++MA+KISYE +PY+ SVV + W+M+L+G +DF+N FQ
Sbjct: 128 DRRIELDEKINVG-SIEYKSMLSIMASKISYESKPYITSVVKNTWKMDLVGNYDFYNAFQ 187

Query: 189 RKATTQAFMFETTATGPNVTVVAFRGTSPLDAYDWQVDADFSWYEIEGVGRMHSGFMKAL 248
               TQAF+F+T++T P++ VV+FRGT P +A DW  D D SWYE++ VG++H+GF +AL
Sbjct: 188 ESKLTQAFVFKTSSTNPDLIVVSFRGTEPFEAADWCTDLDLSWYEMKNVGKVHAGFSRAL 247

Query: 249 GLQKTTGWPKELPAGAQHDFAYYTLRQKLRDIARANHNARFIITGHSLGGALATVFVTIL 308
           GLQK  GWPKE      H +AYYT+RQ LRD    N N ++I+TGHSLGGALA +F  IL
Sbjct: 248 GLQK-DGWPKE-NISLLHQYAYYTIRQMLRDKLGRNKNLKYILTGHSLGGALAALFPAIL 307

Query: 309 ALHDESSILEKLEGVYTYGQPRVGNRDFAKFMVDTVQKYSFKYYRYVYSSDLVPRVP-SD 368
           A+H E  +L+KLEG+YT+GQPRVG+ DF +FM   V+K+  +Y R+VY++D+VPRVP  D
Sbjct: 308 AIHGEDELLDKLEGIYTFGQPRVGDEDFGEFMKGVVKKHGIEYERFVYNNDVVPRVPFDD 367

Query: 369 GIFFKYRHFGKVVYFNSLYRGRILKEQPYKNYISLFWAIPKFLSAWWEVIRSWIIPFVRG 428
              F Y+H+G    FNSLY+G++ ++ P  NY +L W IP+ L+  WE IRS+I+ F +G
Sbjct: 368 KYLFSYKHYGPCNSFNSLYKGKVREDAPNANYFNLLWLIPQLLTGLWEFIRSFILQFWKG 427

Query: 429 FDYYETLLMRGARVVGLLVPGVAAHFPVNYVNITRLGKL 466
            +Y E  LMR  RVVG++ PG + HFP +YVN TRLG L
Sbjct: 428 DEYKENWLMRFVRVVGIVFPGGSNHFPFDYVNSTRLGGL 462

BLAST of Spg016191 vs. TAIR 10
Match: AT5G42930.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 410.6 bits (1054), Expect = 1.7e-114
Identity = 201/461 (43.60%), Postives = 304/461 (65.94%), Query Frame = 0

Query: 10  SRNYLILKPENANILDLFLFLLPFGWRRRRFIDCS-KGLEDSLRKFTDRLSIVISMVLQI 69
           ++NY +L P  A + DL   L       R+FI  S + +ED L +F  R  I +S+V+Q 
Sbjct: 4   TKNYFVLDPREATVSDLIHLLFSSDLGDRKFIHSSEERIEDDLSRFRRRWIIFVSIVIQK 63

Query: 70  ILLAFATPLAKLDSFLLNLFNFISFNGGVLGLFFKILTVKKLLWPDKNSPEYMSLAAFTD 129
           +++ F  PL  +   L    N +S NGG L +    L    ++WP+K S  + SL    D
Sbjct: 64  LIILFKKPLYFVGFSLACWLNLLSSNGGFL-MILPNLFKGNIIWPEKASATFASLNGNLD 123

Query: 130 WRRDLDSSIEPSDTFRYYSALTVMAAKISYEFQPYVQSVVNHRWRMNLLGYFDFWNDFQR 189
            R +L+  +E     RY + L++MA+K+SYE   +V SV+++ W+M+LLG++  WN +Q+
Sbjct: 124 RRVELNPKVERGSK-RYKAMLSIMASKLSYENINFVSSVLHNHWKMDLLGFYSCWNGYQK 183

Query: 190 KATTQAFMFETTATGPNVTVVAFRGTSPLDAYDWQVDADFSWYEIEGVGRMHSGFMKALG 249
           + +T+  + + T+T PN+ +V+FRGT P DA DW  D D SWYE++ VG++H GFMKALG
Sbjct: 184 QRSTEVIVIKDTSTDPNLIIVSFRGTDPFDADDWCTDLDLSWYEVKNVGKIHGGFMKALG 243

Query: 250 LQKTTGWPKEL----PAGAQHDFAYYTLRQKLRDIARANHNARFIITGHSLGGALATVFV 309
           LQK  GWPKE+       A   +AYYT+R+ L++I   N  ++FI+TGHSLGGALA +F 
Sbjct: 244 LQK-EGWPKEVNLDETQNATTLYAYYTVRRHLKEILDQNPTSKFILTGHSLGGALAILFT 303

Query: 310 TILALHDESSILEKLEGVYTYGQPRVGNRDFAKFMVDTVQKYSFKYYRYVYSSDLVPRVP 369
            +L +HDE  +LE+LEGVYT+GQPRVG+ +F  FM D+++K+  KY RYVY +D+VPR+P
Sbjct: 304 AVLVMHDEEQMLERLEGVYTFGQPRVGDEEFGNFMKDSLKKFDVKYERYVYCNDMVPRLP 363

Query: 370 SDGIFFKYRHFGKVVYFNSLYRGRILKEQPYKNYISLFWAIPKFLSAWWEVIRSWIIPFV 429
            D     ++HFG  +Y++S Y+G++ +E+P KNY +L W +PK ++A WE+IRS+++P+ 
Sbjct: 364 FDDKTLMFKHFGACLYYDSFYKGKVEEEEPNKNYFNLVWVLPKIMNALWELIRSFVMPYW 423

Query: 430 RGFDYYETLLMRGARVVGLLVPGVAAHFPVNYVNITRLGKL 466
           +G ++ E   +R  RVV LL+PG+ AHFP  Y+N+T LG L
Sbjct: 424 KGGEFREGWFLRCFRVVALLIPGLPAHFPNEYINVTLLGDL 461

BLAST of Spg016191 vs. TAIR 10
Match: AT1G56630.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 370.5 bits (950), Expect = 2.0e-102
Identity = 190/460 (41.30%), Postives = 289/460 (62.83%), Query Frame = 0

Query: 11  RNYLILKPENANILDLFLFLLPFGWRRRRFIDCS-KGLEDSLRKFTDRLSIVISMVLQII 70
           +NY +L P  A + DL   L       R+F+D S + LE+ L +F  R  I +S+V+Q +
Sbjct: 5   KNYFVLDPREATVTDLMRLLFYSDLENRKFVDTSVENLENRLCEFRGRWIIFVSIVVQKL 64

Query: 71  LLAFATPLAKLDSFLLNLFNFISFNGGVLGLFFKILTVKKLLWPDKNSPEYMSLAAFTDW 130
           L+    PL+ L   L    N  S NGG   +F   L   + +WP+K S  + S+    D 
Sbjct: 65  LIILRKPLSFLGFALGFWLNLPSSNGGFFKIFLN-LVKGRFIWPEKTSATFASINGNLDQ 124

Query: 131 RRD--LDSSIEPSDTFRYYSALTVMAAKISYEFQPYVQSVVNHRWRMNLLGYFDFWNDFQ 190
           + +  L  SI+  D  RY   L++MA+K++YE + +++SV+   W+M+LLG++   NDF 
Sbjct: 125 KVELGLGRSIKIGDE-RYKPLLSIMASKLAYENEHFIRSVLQDHWQMDLLGFYSCPNDFD 184

Query: 191 RKATTQAFMFETTATGPNVTVVAFRGTSPLDAYDWQVDADFSWYEIEGVGRMHSGFMKAL 250
           +  +T+  +   T   PN+ VV+FRGT P +A DW  D D SW+ +  VG++H GFMKAL
Sbjct: 185 QTRSTEVIVIRDTKDNPNLIVVSFRGTDPFNADDWCTDLDLSWHNVMNVGKIHGGFMKAL 244

Query: 251 GLQKTTGWPKELPAGAQHD----FAYYTLRQKLRDIARANHNARFIITGHSLGGALATVF 310
           GL K  GW +E+ +    +     AYYT+ ++L+++   N  ++FI++GHSLGGALA +F
Sbjct: 245 GLPK-EGWYEEINSDQTQNKTSQLAYYTILRQLKEVFEQNPTSKFILSGHSLGGALAILF 304

Query: 311 VTILALHDESSILEKLEGVYTYGQPRVGNRDFAKFMVDTVQKYSFKYYRYVYSSDLVPRV 370
             +L +HDE  +LE+LEGVYT+GQPRVG+ DF  +M D ++++  KY RYVY +D+VPR+
Sbjct: 305 TAVLIMHDEKEMLERLEGVYTFGQPRVGDEDFGIYMKDKLKEFDVKYKRYVYCNDMVPRL 364

Query: 371 PSDGIFFKYRHFGKVVYFNSLYRGRILKEQPYKNYISLFWAIPKFLSAWWEVIRSWIIPF 430
           P D     ++HFG  +Y +S Y+G++ +E+P KNY ++FW IPK ++A WE+IRS+II  
Sbjct: 365 PFDDKTLMFKHFGGCLYCDSFYKGKVEEEEPNKNYFNIFWVIPKIINAVWELIRSFIISC 424

Query: 431 VRGFDYYETLLMRGARVVGLLVPGVAAHFPVNYVNITRLG 464
            +G +Y E  L+   R+V LL+PG+ AHFP  YVN+  LG
Sbjct: 425 WQGREYREGWLLTCFRLVALLIPGLPAHFPNEYVNVALLG 461

BLAST of Spg016191 vs. TAIR 10
Match: AT5G67050.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 350.9 bits (899), Expect = 1.6e-96
Identity = 191/470 (40.64%), Postives = 279/470 (59.36%), Query Frame = 0

Query: 1   MGSDQEHAFSRNYLILKPENANILDLFLFLLPFGWRRRRFIDCSKGLEDSLRKFTDRLSI 60
           M SD +    R YLIL+PE     +L   L      + R +D S+  E S   F  R  I
Sbjct: 2   MNSDDDDE-PRGYLILRPEELRPWELVRLLFSGDIEKPRSVDSSETEEHS---FRHRWLI 61

Query: 61  VISMVLQIILLAFATPLAKLDSFLLNLFNFISFNGGVLGLFFKILTVKKLLWPDKNSPEY 120
            +S+VL  +L  F+  LA + S L    NF+S N    GLF +     +++ P + S  Y
Sbjct: 62  FVSLVLLKLLRFFSKLLALVGSALEFSLNFLS-NNSFSGLFLR----GEVVMPQRTSENY 121

Query: 121 MSLAAFTDWRRDLDSSIEPSDTFRYYSALTVMAAKISYEFQPYVQSVVNHRWRMNLLGYF 180
            S     D R  LD ++   D  +YY+AL++MA+KI+YE    ++ VV + W M  LG  
Sbjct: 122 QSFIGHLDTRVSLDMTLNREDGEKYYAALSIMASKIAYENAARIKHVVENHWNMKYLGLV 181

Query: 181 DFWNDFQRKATTQAFMFET--TATGPN----VTVVAFRGTSPLDAYDWQVDADFSWYEIE 240
           D+WN++Q K TTQAF+  T  T T  N      VVAFRGT   ++ DW  D D +W+E+ 
Sbjct: 182 DYWNEYQEKETTQAFIMSTDETTTRSNGQETTVVVAFRGTELFNSEDWCSDFDITWFELP 241

Query: 241 GVGRMHSGFMKALGLQKTTGWPKELPAG--AQHDFAYYTLRQKLRDIARANHNARFIITG 300
            +G +H GFMKALGLQ    WPKE  +    +   AYY++R  L+ +   N N +F++TG
Sbjct: 242 NIGNIHGGFMKALGLQNNCSWPKEPLSNPDRKSPLAYYSIRDSLKTLIAQNKNTKFVLTG 301

Query: 301 HSLGGALATVFVTILALHDESSILEKLEGVYTYGQPRVGNRDFAKFMVDTVQKYSFKYYR 360
           HSLGGALA +F  +L +H E+ +LE+++GVYTYGQPRVG+  F +FM   ++KY+ KYYR
Sbjct: 302 HSLGGALAILFTAVLVIHHETELLERIQGVYTYGQPRVGDSKFGEFMEKKLEKYNIKYYR 361

Query: 361 YVYSSDLVPRVPSDGIFFKYRHFGKVVYFNSLYRGRILKEQPYKNYISLFWAIPKFLSAW 420
           +VY++D+VPR+P D     ++HFG  +Y++  Y+ ++++EQ  +N+  L   I    SA 
Sbjct: 362 FVYNNDIVPRLPYDDKDLMFKHFGTCIYYDQNYQAKVMREQSDENFFLLRGIIKMMWSAI 421

Query: 421 WEVIRSWIIPFVRGFDYYETLLMRGARVVGLLVPGVAAHFPVNYVNITRL 463
            E IRS+ I   +G +Y E  L++G R +G++VPGV+ H P +YVN TRL
Sbjct: 422 LEFIRSFTIVAEKGSEYSEGWLLKGGRALGIIVPGVSNHTPQDYVNATRL 462

BLAST of Spg016191 vs. TAIR 10
Match: AT1G45201.2 (triacylglycerol lipase-like 1 )

HSP 1 Score: 338.6 bits (867), Expect = 8.4e-93
Identity = 174/383 (45.43%), Postives = 247/383 (64.49%), Query Frame = 0

Query: 9   FSRNYLILKPENANILDLFLFLLPFGWRRRRFIDCSKG-LEDSLRKFTDRLSIVISMVLQ 68
           F  +Y ++ P  A+ LDL L L        RFID     L+   R F  R  + +++ LQ
Sbjct: 8   FCNSYFLVDPTKASFLDLLLLLFSSNLTSARFIDSPPDTLKGFRRSFASRWILALAIFLQ 67

Query: 69  IILLAFATPLAKLDSFLLNLFNFISFNGGVLGLFFKILTVKKLLWPDKNSPEYMSLAAFT 128
            +L+  + P A +   L    N ++ NGG   L   +++  KL+ PDK+S  Y S    +
Sbjct: 68  KVLMLLSKPFAFIGQKLTYWLNLLTANGGFFNLILNLMS-GKLVKPDKSSATYTSFIGCS 127

Query: 129 DWRRDLDSSIEPSDTFRYYSALTVMAAKISYEFQPYVQSVVNHRWRMNLLGYFDFWNDFQ 188
           D R +LD  I    +  Y S L++MA+KISYE +PY+ SVV + W+M+L+G +DF+N FQ
Sbjct: 128 DRRIELDEKINVG-SIEYKSMLSIMASKISYESKPYITSVVKNTWKMDLVGNYDFYNAFQ 187

Query: 189 RKATTQAFMFETTATGPNVTVVAFRGTSPLDAYDWQVDADFSWYEIEGVGRMHSGFMKAL 248
               TQAF+F+T++T P++ VV+FRGT P +A DW  D D SWYE++ VG++H+GF +AL
Sbjct: 188 ESKLTQAFVFKTSSTNPDLIVVSFRGTEPFEAADWCTDLDLSWYEMKNVGKVHAGFSRAL 247

Query: 249 GLQKTTGWPKELPAGAQHDFAYYTLRQKLRDIARANHNARFIITGHSLGGALATVFVTIL 308
           GLQK  GWPKE      H +AYYT+RQ LRD    N N ++I+TGHSLGGALA +F  IL
Sbjct: 248 GLQK-DGWPKE-NISLLHQYAYYTIRQMLRDKLGRNKNLKYILTGHSLGGALAALFPAIL 307

Query: 309 ALHDESSILEKLEGVYTYGQPRVGNRDFAKFMVDTVQKYSFKYYRYVYSSDLVPRVP-SD 368
           A+H E  +L+KLEG+YT+GQPRVG+ DF +FM   V+K+  +Y R+VY++D+VPRVP  D
Sbjct: 308 AIHGEDELLDKLEGIYTFGQPRVGDEDFGEFMKGVVKKHGIEYERFVYNNDVVPRVPFDD 367

Query: 369 GIFFKYRHFGKVVYFNSLYRGRI 390
              F Y+H+G    FNSLY+G++
Sbjct: 368 KYLFSYKHYGPCNSFNSLYKGKV 386