Spg016134 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg016134 vs. NCBI nr
Match: XP_038898702.1 (aspartic proteinase 39-like [Benincasa hispida])

HSP 1 Score: 781.9 bits (2018), Expect = 3.0e-222
Identity = 385/450 (85.56%), Postives = 398/450 (88.44%), Query Frame = 0

Query: 1   MSPPCANSPSAIVVYCLVGFNLLATIFSSSLGSRDFDYQQRPVILPLYISSTNSTHQRVL 60
           MSPP A+S SAIV YCLVGFNLL  I SSS+ +RDFDYQQ+PVILPLYIS  NSTHQRVL
Sbjct: 41  MSPPWADSYSAIVFYCLVGFNLLGRILSSSVHARDFDYQQQPVILPLYISPMNSTHQRVL 100

Query: 61  DRDHRLRHLQNLDKPHSSNARMRLHDDLLTNGFVFQNFSASVSVFGLFTCSLRIGGSGRL 120
           DRDHRLRHLQNL KPHSSNARMRLHDDLLTNG                            
Sbjct: 101 DRDHRLRHLQNLGKPHSSNARMRLHDDLLTNG---------------------------- 160

Query: 121 YRYYTTRLWIGTPPQEFALIVDTGSTVTYVPCSNCVECGNHQSITTGHNDPRFQPELSST 180
             YYTTRLWIGTPPQEFALIVDTGSTVTYVPCSNCVECGNHQ       DPRFQPELSST
Sbjct: 161 --YYTTRLWIGTPPQEFALIVDTGSTVTYVPCSNCVECGNHQ-------DPRFQPELSST 220

Query: 181 YQPVKCNIDCNCDDNGVQCTYERRYAEMSTSSGVLAEDIMSFGKESELVPQRAVFGCETM 240
           YQPVKCN+DCNCDDNGVQCTYERRYAEMSTSSGVLAEDIMSFGKESELVPQRAVFGCETM
Sbjct: 221 YQPVKCNVDCNCDDNGVQCTYERRYAEMSTSSGVLAEDIMSFGKESELVPQRAVFGCETM 280

Query: 241 ETGDLYTQRADGIMGLGRGTLSVMDQLVDKGVVSNSFSLCYGGMDVGGGAMVLGGISSPP 300
           ETGDLYTQRADGIMGLGRGTLSVMDQLV+K V+SNSFSLCYGGMDVGGGAMVLGGISSPP
Sbjct: 281 ETGDLYTQRADGIMGLGRGTLSVMDQLVEKRVISNSFSLCYGGMDVGGGAMVLGGISSPP 340

Query: 301 GMVFTHSNPSRSPYYNIELKEIHVAGKPLKLNPTTFDGKYGTVLDSGTTYAYFPEKAYYA 360
           GMVFTHS+PSRSPYYNIELKEIHVAGKPLKLNP+TFDGKYG +LDSGTTYAYFPEKAYYA
Sbjct: 341 GMVFTHSDPSRSPYYNIELKEIHVAGKPLKLNPSTFDGKYGAILDSGTTYAYFPEKAYYA 400

Query: 361 FKDAIMKKISFLKRINGPDPNFNDICFSGAGRDVNELSKVFPEVDMVFANGQKISLSPEN 420
           FKDAIMKKISFLK+I GPDPNF DICFSGAGRDV ELSKVFPEVDMVFANGQKISLSPEN
Sbjct: 401 FKDAIMKKISFLKQIGGPDPNFKDICFSGAGRDVTELSKVFPEVDMVFANGQKISLSPEN 453

Query: 421 YLFRHTKVSGAYCLGIFKNGNDQTTLLGGM 451
           YLFRHTKVSGAYCLG+FKNGNDQTTLLGG+
Sbjct: 461 YLFRHTKVSGAYCLGVFKNGNDQTTLLGGI 453

BLAST of Spg016134 vs. NCBI nr
Match: KAG7024286.1 (Aspartic proteinase-like protein 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 781.2 bits (2016), Expect = 5.1e-222
Identity = 386/457 (84.46%), Postives = 402/457 (87.96%), Query Frame = 0

Query: 1   MSPPCANSPSAIVVYCLVGFNLLATIFSSSLGSRDFDYQQRPVILPLYISSTNSTHQRVL 60
           MSPP  +  SAI VYCLVGFNLL  IFSSS+ SRDFDYQQRPVILPLYIS TNSTHQRVL
Sbjct: 1   MSPPSTSLHSAIFVYCLVGFNLLGAIFSSSVDSRDFDYQQRPVILPLYISPTNSTHQRVL 60

Query: 61  DRDHRLRHLQNLDKPHSSNARMRLHDDLLTNGFVFQNFSASVSVFGLFTCSLRIGGSGRL 120
           DRDH LRHL NL+KPHSSNARMRL+DDLLTNG                            
Sbjct: 61  DRDHSLRHLLNLEKPHSSNARMRLYDDLLTNG---------------------------- 120

Query: 121 YRYYTTRLWIGTPPQEFALIVDTGSTVTYVPCSNCVECGNHQSITTGHNDPRFQPELSST 180
             YYTTRLWIGTPPQEFALIVDTGSTVTYVPCSNCV+CGNHQ       DPRFQP+LSS+
Sbjct: 121 --YYTTRLWIGTPPQEFALIVDTGSTVTYVPCSNCVDCGNHQ-------DPRFQPKLSSS 180

Query: 181 YQPVKCNIDCNCDDNGVQCTYERRYAEMSTSSGVLAEDIMSFGKESELVPQRAVFGCETM 240
           YQPVKCNIDC+CDDNGVQCTYERRYAEMSTSSGVLAEDIMSFGKESEL+PQRAVFGCETM
Sbjct: 181 YQPVKCNIDCSCDDNGVQCTYERRYAEMSTSSGVLAEDIMSFGKESELLPQRAVFGCETM 240

Query: 241 ETGDLYTQRADGIMGLGRGTLSVMDQLVDKGVVSNSFSLCYGGMDVGGGAMVLGGISSPP 300
           ETGDLYTQRADGIMGLGRGTLSVMDQLV KG VSNSFSLCYGGMDVGGGAMVLGGISSPP
Sbjct: 241 ETGDLYTQRADGIMGLGRGTLSVMDQLVGKGAVSNSFSLCYGGMDVGGGAMVLGGISSPP 300

Query: 301 GMVFTHSNPSRSPYYNIELKEIHVAGKPLKLNPTTFDGKYGTVLDSGTTYAYFPEKAYYA 360
           GMVFTHS+ SRSPYYNIELKEIHVAGKPLKLNP TFDGKYG+VLDSGTTYAYFPEKAYYA
Sbjct: 301 GMVFTHSDTSRSPYYNIELKEIHVAGKPLKLNPRTFDGKYGSVLDSGTTYAYFPEKAYYA 360

Query: 361 FKDAIMKKISFLKRINGPDPNFNDICFSGAGRDVNELSKVFPEVDMVFANGQKISLSPEN 420
           FKDA+MKKISFLK+INGPDPNF DICFSGAGRDV+ELSKVFPEV MVFANGQKISLSPEN
Sbjct: 361 FKDALMKKISFLKQINGPDPNFKDICFSGAGRDVSELSKVFPEVAMVFANGQKISLSPEN 420

Query: 421 YLFRHTKVSGAYCLGIFKNGNDQTTLLGGMAKFPGLL 458
           YLFRHTKVSGAYCLGIFKNGNDQTTLLGGMAKFP ++
Sbjct: 421 YLFRHTKVSGAYCLGIFKNGNDQTTLLGGMAKFPWIV 420

BLAST of Spg016134 vs. NCBI nr
Match: XP_022139880.1 (aspartic proteinase-like protein 2 [Momordica charantia])

HSP 1 Score: 776.2 bits (2003), Expect = 1.6e-220
Identity = 381/450 (84.67%), Postives = 398/450 (88.44%), Query Frame = 0

Query: 1   MSPPCANSPSAIVVYCLVGFNLLATIFSSSLGSRDFDYQQRPVILPLYISSTNSTHQRVL 60
           MSP  ANSPSAIV+YCLVGFNLL T+FSSS+ SRDFDYQQRPVILPLYIS+TNSTH RVL
Sbjct: 1   MSPSGANSPSAIVIYCLVGFNLLRTVFSSSVDSRDFDYQQRPVILPLYISTTNSTHHRVL 60

Query: 61  DRDHRLRHLQNLDKPHSSNARMRLHDDLLTNGFVFQNFSASVSVFGLFTCSLRIGGSGRL 120
           +RDHR RHLQ LD+PHSS ARMRLHDDLLTNG                            
Sbjct: 61  NRDHRFRHLQXLDQPHSSTARMRLHDDLLTNG---------------------------- 120

Query: 121 YRYYTTRLWIGTPPQEFALIVDTGSTVTYVPCSNCVECGNHQSITTGHNDPRFQPELSST 180
             YYTTRLWIGTPPQEFALIVDTGSTVTYVPCSNC ECGNHQ       DP+FQPELSST
Sbjct: 121 --YYTTRLWIGTPPQEFALIVDTGSTVTYVPCSNCEECGNHQ-------DPKFQPELSST 180

Query: 181 YQPVKCNIDCNCDDNGVQCTYERRYAEMSTSSGVLAEDIMSFGKESELVPQRAVFGCETM 240
           YQPVKCNI+CNCDD GVQCTYERRYAEMSTSSG+LAEDIMSFGKESELVPQRAVFGCETM
Sbjct: 181 YQPVKCNINCNCDDKGVQCTYERRYAEMSTSSGMLAEDIMSFGKESELVPQRAVFGCETM 240

Query: 241 ETGDLYTQRADGIMGLGRGTLSVMDQLVDKGVVSNSFSLCYGGMDVGGGAMVLGGISSPP 300
           ETGDLYTQRADGIMGLGRG+LSVMDQL +KGVVS+SFSLCYGGMDVGGGAMVLGGISSPP
Sbjct: 241 ETGDLYTQRADGIMGLGRGSLSVMDQLANKGVVSDSFSLCYGGMDVGGGAMVLGGISSPP 300

Query: 301 GMVFTHSNPSRSPYYNIELKEIHVAGKPLKLNPTTFDGKYGTVLDSGTTYAYFPEKAYYA 360
           GMVFTHS+PSRSPYYNIELKEIHVAGKPLKLNP TFDGKYGTVLDSGTTYAYFPEKAYYA
Sbjct: 301 GMVFTHSDPSRSPYYNIELKEIHVAGKPLKLNPNTFDGKYGTVLDSGTTYAYFPEKAYYA 360

Query: 361 FKDAIMKKISFLKRINGPDPNFNDICFSGAGRDVNELSKVFPEVDMVFANGQKISLSPEN 420
           FKDAIMKKI+FLK+I+GPDPNF DICFSGAGRDVNEL KVFPEVD VFANGQKISLSPEN
Sbjct: 361 FKDAIMKKINFLKQISGPDPNFKDICFSGAGRDVNELPKVFPEVDFVFANGQKISLSPEN 413

Query: 421 YLFRHTKVSGAYCLGIFKNGNDQTTLLGGM 451
           YLFRHTKVSGAYCLGIFKNGNDQTTLLGG+
Sbjct: 421 YLFRHTKVSGAYCLGIFKNGNDQTTLLGGI 413

BLAST of Spg016134 vs. NCBI nr
Match: XP_008452619.1 (PREDICTED: aspartic proteinase-like protein 2 [Cucumis melo])

HSP 1 Score: 774.6 bits (1999), Expect = 4.7e-220
Identity = 382/450 (84.89%), Postives = 394/450 (87.56%), Query Frame = 0

Query: 1   MSPPCANSPSAIVVYCLVGFNLLATIFSSSLGSRDFDYQQRPVILPLYISSTNSTHQRVL 60
           MSP C NS SAIV  CLVGFNLL  I SSS+ SRD DYQQRPV+LPLYIS TNSTH+RV 
Sbjct: 1   MSPTCDNSYSAIVFCCLVGFNLLGMILSSSVDSRDLDYQQRPVLLPLYISPTNSTHRRVF 60

Query: 61  DRDHRLRHLQNLDKPHSSNARMRLHDDLLTNGFVFQNFSASVSVFGLFTCSLRIGGSGRL 120
           DRDHRLRHLQNL KPHSSNARMRLHDDLLTNG                            
Sbjct: 61  DRDHRLRHLQNLVKPHSSNARMRLHDDLLTNG---------------------------- 120

Query: 121 YRYYTTRLWIGTPPQEFALIVDTGSTVTYVPCSNCVECGNHQSITTGHNDPRFQPELSST 180
             YYTTRLWIGTPPQEFALIVDTGSTVTYVPCSNCVECGNHQ       DPRFQPELSST
Sbjct: 121 --YYTTRLWIGTPPQEFALIVDTGSTVTYVPCSNCVECGNHQ-------DPRFQPELSST 180

Query: 181 YQPVKCNIDCNCDDNGVQCTYERRYAEMSTSSGVLAEDIMSFGKESELVPQRAVFGCETM 240
           YQPVKCN+DCNCD+NGVQCTYERRYAEMSTSSGVLAED+MSFGKESELVPQRAVFGCETM
Sbjct: 181 YQPVKCNVDCNCDENGVQCTYERRYAEMSTSSGVLAEDVMSFGKESELVPQRAVFGCETM 240

Query: 241 ETGDLYTQRADGIMGLGRGTLSVMDQLVDKGVVSNSFSLCYGGMDVGGGAMVLGGISSPP 300
           ETGDLYTQRADGIMGLGRGTLSVMDQLV KGVVSNSFSLCYGGMDVGGGAMVLGGISSPP
Sbjct: 241 ETGDLYTQRADGIMGLGRGTLSVMDQLVGKGVVSNSFSLCYGGMDVGGGAMVLGGISSPP 300

Query: 301 GMVFTHSNPSRSPYYNIELKEIHVAGKPLKLNPTTFDGKYGTVLDSGTTYAYFPEKAYYA 360
           GMVF+HS+PSRSPYYNIELKEIHVAGKPLKLNP TFDGKYG +LDSGTTYAYFPEKAYYA
Sbjct: 301 GMVFSHSDPSRSPYYNIELKEIHVAGKPLKLNPRTFDGKYGAILDSGTTYAYFPEKAYYA 360

Query: 361 FKDAIMKKISFLKRINGPDPNFNDICFSGAGRDVNELSKVFPEVDMVFANGQKISLSPEN 420
           FKDAIMKKISFLK+INGPDPNF DICFSGAGRDV EL KVFPEVDMVFA+GQKISLSPEN
Sbjct: 361 FKDAIMKKISFLKQINGPDPNFKDICFSGAGRDVTELPKVFPEVDMVFADGQKISLSPEN 413

Query: 421 YLFRHTKVSGAYCLGIFKNGNDQTTLLGGM 451
           YLFRHTKVSGAYCLGIFKNGNDQTTLLGG+
Sbjct: 421 YLFRHTKVSGAYCLGIFKNGNDQTTLLGGI 413

BLAST of Spg016134 vs. NCBI nr
Match: XP_023535922.1 (aspartic proteinase-like protein 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 771.9 bits (1992), Expect = 3.1e-219
Identity = 381/450 (84.67%), Postives = 397/450 (88.22%), Query Frame = 0

Query: 1   MSPPCANSPSAIVVYCLVGFNLLATIFSSSLGSRDFDYQQRPVILPLYISSTNSTHQRVL 60
           MSPP  +  SAI VYCLVGFNLL  IFSSS+ SRDFDYQQRPVILPLYIS TNSTHQRVL
Sbjct: 1   MSPPSTSLHSAIFVYCLVGFNLLGAIFSSSVDSRDFDYQQRPVILPLYISPTNSTHQRVL 60

Query: 61  DRDHRLRHLQNLDKPHSSNARMRLHDDLLTNGFVFQNFSASVSVFGLFTCSLRIGGSGRL 120
           DRDHRLRHL NL+KPHSSNARMRL+DDLLTNG                            
Sbjct: 61  DRDHRLRHLLNLEKPHSSNARMRLYDDLLTNG---------------------------- 120

Query: 121 YRYYTTRLWIGTPPQEFALIVDTGSTVTYVPCSNCVECGNHQSITTGHNDPRFQPELSST 180
             YYTTRLWIGTPPQEFALIVDTGSTVTYVPCSNCV+CGNHQ       DPRFQP+LSS+
Sbjct: 121 --YYTTRLWIGTPPQEFALIVDTGSTVTYVPCSNCVDCGNHQ-------DPRFQPKLSSS 180

Query: 181 YQPVKCNIDCNCDDNGVQCTYERRYAEMSTSSGVLAEDIMSFGKESELVPQRAVFGCETM 240
           YQPVKCNIDC+CDDNGVQCTYERRYAEMSTSSGVLAEDIMSFGKESEL+PQRAVFGCETM
Sbjct: 181 YQPVKCNIDCSCDDNGVQCTYERRYAEMSTSSGVLAEDIMSFGKESELLPQRAVFGCETM 240

Query: 241 ETGDLYTQRADGIMGLGRGTLSVMDQLVDKGVVSNSFSLCYGGMDVGGGAMVLGGISSPP 300
           ETGDLYTQRADGIMGLGRGTLSVMDQLV KG VSNSFSLCYGGMDVGGGAMVLGGISSPP
Sbjct: 241 ETGDLYTQRADGIMGLGRGTLSVMDQLVGKGAVSNSFSLCYGGMDVGGGAMVLGGISSPP 300

Query: 301 GMVFTHSNPSRSPYYNIELKEIHVAGKPLKLNPTTFDGKYGTVLDSGTTYAYFPEKAYYA 360
           GMVFTHS+ +RSPYYNIELKEIHVAGKPLKLNP TFDGKYG+VLDSGTTYAYFPEKAYYA
Sbjct: 301 GMVFTHSDTTRSPYYNIELKEIHVAGKPLKLNPRTFDGKYGSVLDSGTTYAYFPEKAYYA 360

Query: 361 FKDAIMKKISFLKRINGPDPNFNDICFSGAGRDVNELSKVFPEVDMVFANGQKISLSPEN 420
           FKDA+MKKISFLK+INGPDPNF DICFSGAGRDV+ELSKVFPEV MVFANGQKISLSPEN
Sbjct: 361 FKDALMKKISFLKQINGPDPNFKDICFSGAGRDVSELSKVFPEVAMVFANGQKISLSPEN 413

Query: 421 YLFRHTKVSGAYCLGIFKNGNDQTTLLGGM 451
           YLFRHTKVSGAYCLGIFKNGNDQTTLLGG+
Sbjct: 421 YLFRHTKVSGAYCLGIFKNGNDQTTLLGGI 413

BLAST of Spg016134 vs. ExPASy Swiss-Prot
Match: Q4V3D2 (Aspartic proteinase 36 OS=Arabidopsis thaliana OX=3702 GN=A36 PE=1 SV=1)

HSP 1 Score: 138.7 bits (348), Expect = 1.7e-31
Identity = 105/347 (30.26%), Postives = 167/347 (48.13%), Query Frame = 0

Query: 107 LFTCSLRIGGSGRL--YRYYTTRLWIGTPPQEFALIVDTGSTVTYVPCSNCVECGNHQSI 166
           L    L +GG  R      Y T++ +G+PP+E+ + VDTGS + +V C+ C +C     +
Sbjct: 59  LANIDLPLGGDSRADSIGLYFTKIKLGSPPKEYYVQVDTGSDILWVNCAPCPKCPVKTDL 118

Query: 167 TTGHNDPRFQPELSSTYQPVKCNID-----CNCDDNGVQ--CTYERRYAEMSTSSGVLAE 226
             G     +  + SST + V C  D        +  G +  C+Y   Y + STS G   +
Sbjct: 119 --GIPLSLYDSKTSSTSKNVGCEDDFCSFIMQSETCGAKKPCSYHVVYGDGSTSDGDFIK 178

Query: 227 DIMSFGK-----ESELVPQRAVFGCETMETGDL--YTQRADGIMGLGRGTLSVMDQLVDK 286
           D ++  +      +  + Q  VFGC   ++G L       DGIMG G+   S++ QL   
Sbjct: 179 DNITLEQVTGNLRTAPLAQEVVFGCGKNQSGQLGQTDSAVDGIMGFGQSNTSIISQLAAG 238

Query: 287 GVVSNSFSLCYGGMDVGGGAMVLGGISSPPGMVFTHSNPSRSPYYNIELKEIHVAGKPLK 346
           G     FS C   M+ GGG   +G + SP  +V T        +YN+ LK + V G P+ 
Sbjct: 239 GSTKRIFSHCLDNMN-GGGIFAVGEVESP--VVKTTPIVPNQVHYNVILKGMDVDGDPID 298

Query: 347 LNPT--TFDGKYGTVLDSGTTYAYFPEKAYYAFKDAIMKKISFLKRINGPDPNFNDICFS 406
           L P+  + +G  GT++DSGTT AY P+  Y    +++++KI+  +++          CFS
Sbjct: 299 LPPSLASTNGDGGTIIDSGTTLAYLPQNLY----NSLIEKITAKQQVKLHMVQETFACFS 358

Query: 407 GAGRDVNELSKVFPEVDMVFANGQKISLSPENYLFRHTKVSGAYCLG 436
                 +   K FP V++ F +  K+S+ P +YLF  +     YC G
Sbjct: 359 ----FTSNTDKAFPVVNLHFEDSLKLSVYPHDYLF--SLREDMYCFG 390

BLAST of Spg016134 vs. ExPASy Swiss-Prot
Match: Q9S9K4 (Aspartic proteinase 39 OS=Arabidopsis thaliana OX=3702 GN=A39 PE=1 SV=2)

HSP 1 Score: 134.4 bits (337), Expect = 3.3e-30
Identity = 106/374 (28.34%), Postives = 172/374 (45.99%), Query Frame = 0

Query: 107 LFTCSLRIGGSGRL--YRYYTTRLWIGTPPQEFALIVDTGSTVTYVPCSNCVECGNHQSI 166
           L +  L +GG  R+     Y T++ +G+PP+E+ + VDTGS + ++ C  C +C    ++
Sbjct: 55  LASIDLPLGGDSRVDSVGLYFTKIKLGSPPKEYHVQVDTGSDILWINCKPCPKCPTKTNL 114

Query: 167 TTGHNDPRFQPELSSTYQPVKCNID-------CNCDDNGVQCTYERRYAEMSTSSGVLAE 226
                   F    SST + V C+ D        +     + C+Y   YA+ STS G    
Sbjct: 115 --NFRLSLFDMNASSTSKKVGCDDDFCSFISQSDSCQPALGCSYHIVYADESTSDGKFIR 174

Query: 227 DIMSFGK-----ESELVPQRAVFGCETMETGDLYT--QRADGIMGLGRGTLSVMDQLVDK 286
           D+++  +     ++  + Q  VFGC + ++G L       DG+MG G+   SV+ QL   
Sbjct: 175 DMLTLEQVTGDLKTGPLGQEVVFGCGSDQSGQLGNGDSAVDGVMGFGQSNTSVLSQLAAT 234

Query: 287 GVVSNSFSLCYGGMDVGGGAMVLGGISSPPGMVFTHSNPSRSPYYNIELKEIHVAGKPLK 346
           G     FS C   +  GGG   +G + SP   V T        +YN+ L  + V G  L 
Sbjct: 235 GDAKRVFSHCLDNVK-GGGIFAVGVVDSP--KVKTTPMVPNQMHYNVMLMGMDVDGTSLD 294

Query: 347 LNPTTFDGKYGTVLDSGTTYAYFPEKAYYAFKDAIMKKISFLKRINGPDPNFNDICFSGA 406
           L P +     GT++DSGTT AYFP+  Y +  + I+ +     +++  +  F   CFS +
Sbjct: 295 L-PRSIVRNGGTIVDSGTTLAYFPKVLYDSLIETILARQPV--KLHIVEETFQ--CFSFS 354

Query: 407 GRDVNELSKVFPEVDMVFANGQKISLSPENYLFRHTKVSGAYCLGIFKNG-----NDQTT 460
                 + + FP V   F +  K+++ P +YLF  T     YC G    G       +  
Sbjct: 355 ----TNVDEAFPPVSFEFEDSVKLTVYPHDYLF--TLEEELYCFGWQAGGLTTDERSEVI 412

BLAST of Spg016134 vs. ExPASy Swiss-Prot
Match: Q9LHE3 (Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASPG2 PE=2 SV=1)

HSP 1 Score: 127.5 bits (319), Expect = 4.0e-28
Identity = 125/442 (28.28%), Postives = 188/442 (42.53%), Query Frame = 0

Query: 28  SSSLGSRDF---DYQQRPVILPLYISSTNSTH----------QRVLDRDHRLRHLQNLDK 87
           SSS+   DF   D  Q P+ +   +   N+TH           R+L RD R   +   + 
Sbjct: 19  SSSISFPDFQIIDVLQPPLTVTATLPDFNNTHFSDESSSKYTLRLLHRD-RFPSVTYRNH 78

Query: 88  PHSSNARMRLHDDLLT------NGFVFQNFSA--SVSVFGLFTCSLRIGGSGRLYRYYTT 147
            H  +ARMR   D ++      +G V  +  +   V+ FG    S    GSG     Y  
Sbjct: 79  HHRLHARMRRDTDRVSAILRRISGKVIPSSDSRYEVNDFGSDIVSGMDQGSGE----YFV 138

Query: 148 RLWIGTPPQEFALIVDTGSTVTYVPCSNCVECGNHQSITTGHNDPRFQPELSSTYQPVKC 207
           R+ +G+PP++  +++D+GS + +V C  C  C          +DP F P  S +Y  V C
Sbjct: 139 RIGVGSPPRDQYMVIDSGSDMVWVQCQPCKLCYK-------QSDPVFDPAKSGSYTGVSC 198

Query: 208 NI-------DCNCDDNGVQCTYERRYAEMSTSSGVLAEDIMSFGKESELVPQRAVFGCET 267
                    +  C   G  C YE  Y + S + G LA + ++F K    V +    GC  
Sbjct: 199 GSSVCDRIENSGCHSGG--CRYEVMYGDGSYTKGTLALETLTFAK---TVVRNVAMGCGH 258

Query: 268 METGDLYTQRADGIMGLGRGTLSVMDQLVDKGVVSNSFSLCY--GGMDVGGGAMVLGGIS 327
              G      A G++G+G G++S + QL   G    +F  C    G D   G++V G  +
Sbjct: 259 RNRGMFI--GAAGLLGIGGGSMSFVGQL--SGQTGGAFGYCLVSRGTD-STGSLVFGREA 318

Query: 328 SPPGMVFTH--SNPSRSPYYNIELKEIHVAGKPLKLNPTTFD----GKYGTVLDSGTTYA 387
            P G  +     NP    +Y + LK + V G  + L    FD    G  G V+D+GT   
Sbjct: 319 LPVGASWVPLVRNPRAPSFYYVGLKGLGVGGVRIPLPDGVFDLTETGDGGVVMDTGTAVT 378

Query: 388 YFPEKAYYAFKDAIMKKISFLKRINGPDPNFNDICFSGAGRDVNELSKVFPEVDMVFANG 434
             P  AY AF+D    + + L R +G   +  D C+  +G     +S   P V   F  G
Sbjct: 379 RLPTAAYVAFRDGFKSQTANLPRASG--VSIFDTCYDLSG----FVSVRVPTVSFYFTEG 431

BLAST of Spg016134 vs. ExPASy Swiss-Prot
Match: Q8VYV9 (Aspartyl protease family protein 1 OS=Arabidopsis thaliana OX=3702 GN=APF1 PE=2 SV=1)

HSP 1 Score: 124.0 bits (310), Expect = 4.4e-27
Identity = 107/379 (28.23%), Postives = 164/379 (43.27%), Query Frame = 0

Query: 73  DKPHSSNARMRLHDDLLTNGFVFQNFSASVSVFGLFTCSLRIGGSGRLYRYYTTRLWIGT 132
           ++  S   R+  H D L  G    N   S+  F     ++R+   G L+    T   +GT
Sbjct: 56  NRDSSKYYRVMAHRDRLIRGRRLANEDQSLVTFSDGNETVRVDALGFLHYANVT---VGT 115

Query: 133 PPQEFALIVDTGSTVTYVPCSNCVECGNHQSITTGHNDPR--FQPELSSTYQPVKCNIDC 192
           P   F + +DTGS + ++PC +C  C        G +     + P  SST   V CN   
Sbjct: 116 PSDWFMVALDTGSDLFWLPC-DCTNCVRELKAPGGSSLDLNIYSPNASSTSTKVPCNSTL 175

Query: 193 -----NCDDNGVQCTYERRYAEMSTSS-GVLAEDIM---SFGKESELVPQRAVFGCETME 252
                 C      C Y+ RY    TSS GVL ED++   S  K S+ +P R  FGC  ++
Sbjct: 176 CTRGDRCASPESDCPYQIRYLSNGTSSTGVLVEDVLHLVSNDKSSKAIPARVTFGCGQVQ 235

Query: 253 TGDLYTQRA-DGIMGLGRGTLSVMDQLVDKGVVSNSFSLCYGGMDVGGGAMVLGGISSPP 312
           TG  +   A +G+ GLG   +SV   L  +G+ +NSFS+C+G  + G G +  G   S  
Sbjct: 236 TGVFHDGAAPNGLFGLGLEDISVPSVLAKEGIAANSFSMCFG--NDGAGRISFGDKGSVD 295

Query: 313 GMVFTHSNPSRSPYYNIELKEIHVAGKPLKLNPTTFDGKYGTVLDSGTTYAYFPEKAYYA 372
                 +     P YNI + +I V G        T D ++  V DSGT++ Y  + AY  
Sbjct: 296 QRETPLNIRQPHPTYNITVTKISVGG-------NTGDLEFDAVFDSGTSFTYLTDAAYTL 355

Query: 373 FKDAIMKKISFLKRINGPDPNFN-DICFSGAGRDVNELSKVFPEVDMVFANGQKISLSPE 432
             ++    ++  KR    D     + C++      N+ S  +P V++    G    +   
Sbjct: 356 ISES-FNSLALDKRYQTTDSELPFEYCYA---LSPNKDSFQYPAVNLTMKGGSSYPVY-H 415

Query: 433 NYLFRHTKVSGAYCLGIFK 439
             +    K +  YCL I K
Sbjct: 416 PLVVIPMKDTDVYCLAIMK 416

BLAST of Spg016134 vs. ExPASy Swiss-Prot
Match: Q9LS40 (Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASPG1 PE=1 SV=1)

HSP 1 Score: 122.9 bits (307), Expect = 9.8e-27
Identity = 102/355 (28.73%), Postives = 161/355 (45.35%), Query Frame = 0

Query: 114 IGGSGRLYRYYTTRLWIGTPPQEFALIVDTGSTVTYVPCSNCVECGNHQSITTGHNDPRF 173
           + G+ +    Y +R+ +GTP +E  L++DTGS V ++ C  C +C          +DP F
Sbjct: 152 VSGASQGSGEYFSRIGVGTPAKEMYLVLDTGSDVNWIQCEPCADCYQ-------QSDPVF 211

Query: 174 QPELSSTYQPVKCNI-DCN------CDDNGVQCTYERRYAEMSTSSGVLAEDIMSFGKES 233
            P  SSTY+ + C+   C+      C  N  +C Y+  Y + S + G LA D ++FG   
Sbjct: 212 NPTSSSTYKSLTCSAPQCSLLETSACRSN--KCLYQVSYGDGSFTVGELATDTVTFGNSG 271

Query: 234 ELVPQRAVFGCETMETGDLYTQRADGIMGLGRGTLSVMDQLVDKGVVSNSFSLCYGGMDV 293
           ++       GC     G L+T  A G++GLG G LS+ +Q+      + SFS C    D 
Sbjct: 272 KI--NNVALGCGHDNEG-LFTGAA-GLLGLGGGVLSITNQM-----KATSFSYCLVDRDS 331

Query: 294 GGGAMV------LGGISSPPGMVFTHSNPSRSPYYNIELKEIHVAGKPLKLNPTTFD--- 353
           G  + +      LGG  +   ++    N     +Y + L    V G+ + L    FD   
Sbjct: 332 GKSSSLDFNSVQLGGGDATAPLL---RNKKIDTFYYVGLSGFSVGGEKVVLPDAIFDVDA 391

Query: 354 -GKYGTVLDSGTTYAYFPEKAYYAFKDAIMKKISFLKRINGPDPNFNDICFSGAGRDVNE 413
            G  G +LD GT       +AY + +DA +K    LK+ +     F D C+     D + 
Sbjct: 392 SGSGGVILDCGTAVTRLQTQAYNSLRDAFLKLTVNLKKGSSSISLF-DTCY-----DFSS 451

Query: 414 LSKV-FPEVDMVFANGQKISLSPENYLFRHTKVSGAYCLGIFKNGNDQTTLLGGM 451
           LS V  P V   F  G+ + L  +NYL      SG +C   F   +   +++G +
Sbjct: 452 LSTVKVPTVAFHFTGGKSLDLPAKNYLI-PVDDSGTFCFA-FAPTSSSLSIIGNV 477

BLAST of Spg016134 vs. ExPASy TrEMBL
Match: A0A6J1CDI9 (aspartic proteinase-like protein 2 OS=Momordica charantia OX=3673 GN=LOC111010681 PE=3 SV=1)

HSP 1 Score: 776.2 bits (2003), Expect = 7.9e-221
Identity = 381/450 (84.67%), Postives = 398/450 (88.44%), Query Frame = 0

Query: 1   MSPPCANSPSAIVVYCLVGFNLLATIFSSSLGSRDFDYQQRPVILPLYISSTNSTHQRVL 60
           MSP  ANSPSAIV+YCLVGFNLL T+FSSS+ SRDFDYQQRPVILPLYIS+TNSTH RVL
Sbjct: 1   MSPSGANSPSAIVIYCLVGFNLLRTVFSSSVDSRDFDYQQRPVILPLYISTTNSTHHRVL 60

Query: 61  DRDHRLRHLQNLDKPHSSNARMRLHDDLLTNGFVFQNFSASVSVFGLFTCSLRIGGSGRL 120
           +RDHR RHLQ LD+PHSS ARMRLHDDLLTNG                            
Sbjct: 61  NRDHRFRHLQXLDQPHSSTARMRLHDDLLTNG---------------------------- 120

Query: 121 YRYYTTRLWIGTPPQEFALIVDTGSTVTYVPCSNCVECGNHQSITTGHNDPRFQPELSST 180
             YYTTRLWIGTPPQEFALIVDTGSTVTYVPCSNC ECGNHQ       DP+FQPELSST
Sbjct: 121 --YYTTRLWIGTPPQEFALIVDTGSTVTYVPCSNCEECGNHQ-------DPKFQPELSST 180

Query: 181 YQPVKCNIDCNCDDNGVQCTYERRYAEMSTSSGVLAEDIMSFGKESELVPQRAVFGCETM 240
           YQPVKCNI+CNCDD GVQCTYERRYAEMSTSSG+LAEDIMSFGKESELVPQRAVFGCETM
Sbjct: 181 YQPVKCNINCNCDDKGVQCTYERRYAEMSTSSGMLAEDIMSFGKESELVPQRAVFGCETM 240

Query: 241 ETGDLYTQRADGIMGLGRGTLSVMDQLVDKGVVSNSFSLCYGGMDVGGGAMVLGGISSPP 300
           ETGDLYTQRADGIMGLGRG+LSVMDQL +KGVVS+SFSLCYGGMDVGGGAMVLGGISSPP
Sbjct: 241 ETGDLYTQRADGIMGLGRGSLSVMDQLANKGVVSDSFSLCYGGMDVGGGAMVLGGISSPP 300

Query: 301 GMVFTHSNPSRSPYYNIELKEIHVAGKPLKLNPTTFDGKYGTVLDSGTTYAYFPEKAYYA 360
           GMVFTHS+PSRSPYYNIELKEIHVAGKPLKLNP TFDGKYGTVLDSGTTYAYFPEKAYYA
Sbjct: 301 GMVFTHSDPSRSPYYNIELKEIHVAGKPLKLNPNTFDGKYGTVLDSGTTYAYFPEKAYYA 360

Query: 361 FKDAIMKKISFLKRINGPDPNFNDICFSGAGRDVNELSKVFPEVDMVFANGQKISLSPEN 420
           FKDAIMKKI+FLK+I+GPDPNF DICFSGAGRDVNEL KVFPEVD VFANGQKISLSPEN
Sbjct: 361 FKDAIMKKINFLKQISGPDPNFKDICFSGAGRDVNELPKVFPEVDFVFANGQKISLSPEN 413

Query: 421 YLFRHTKVSGAYCLGIFKNGNDQTTLLGGM 451
           YLFRHTKVSGAYCLGIFKNGNDQTTLLGG+
Sbjct: 421 YLFRHTKVSGAYCLGIFKNGNDQTTLLGGI 413

BLAST of Spg016134 vs. ExPASy TrEMBL
Match: A0A1S3BTN6 (aspartic proteinase-like protein 2 OS=Cucumis melo OX=3656 GN=LOC103493586 PE=3 SV=1)

HSP 1 Score: 774.6 bits (1999), Expect = 2.3e-220
Identity = 382/450 (84.89%), Postives = 394/450 (87.56%), Query Frame = 0

Query: 1   MSPPCANSPSAIVVYCLVGFNLLATIFSSSLGSRDFDYQQRPVILPLYISSTNSTHQRVL 60
           MSP C NS SAIV  CLVGFNLL  I SSS+ SRD DYQQRPV+LPLYIS TNSTH+RV 
Sbjct: 1   MSPTCDNSYSAIVFCCLVGFNLLGMILSSSVDSRDLDYQQRPVLLPLYISPTNSTHRRVF 60

Query: 61  DRDHRLRHLQNLDKPHSSNARMRLHDDLLTNGFVFQNFSASVSVFGLFTCSLRIGGSGRL 120
           DRDHRLRHLQNL KPHSSNARMRLHDDLLTNG                            
Sbjct: 61  DRDHRLRHLQNLVKPHSSNARMRLHDDLLTNG---------------------------- 120

Query: 121 YRYYTTRLWIGTPPQEFALIVDTGSTVTYVPCSNCVECGNHQSITTGHNDPRFQPELSST 180
             YYTTRLWIGTPPQEFALIVDTGSTVTYVPCSNCVECGNHQ       DPRFQPELSST
Sbjct: 121 --YYTTRLWIGTPPQEFALIVDTGSTVTYVPCSNCVECGNHQ-------DPRFQPELSST 180

Query: 181 YQPVKCNIDCNCDDNGVQCTYERRYAEMSTSSGVLAEDIMSFGKESELVPQRAVFGCETM 240
           YQPVKCN+DCNCD+NGVQCTYERRYAEMSTSSGVLAED+MSFGKESELVPQRAVFGCETM
Sbjct: 181 YQPVKCNVDCNCDENGVQCTYERRYAEMSTSSGVLAEDVMSFGKESELVPQRAVFGCETM 240

Query: 241 ETGDLYTQRADGIMGLGRGTLSVMDQLVDKGVVSNSFSLCYGGMDVGGGAMVLGGISSPP 300
           ETGDLYTQRADGIMGLGRGTLSVMDQLV KGVVSNSFSLCYGGMDVGGGAMVLGGISSPP
Sbjct: 241 ETGDLYTQRADGIMGLGRGTLSVMDQLVGKGVVSNSFSLCYGGMDVGGGAMVLGGISSPP 300

Query: 301 GMVFTHSNPSRSPYYNIELKEIHVAGKPLKLNPTTFDGKYGTVLDSGTTYAYFPEKAYYA 360
           GMVF+HS+PSRSPYYNIELKEIHVAGKPLKLNP TFDGKYG +LDSGTTYAYFPEKAYYA
Sbjct: 301 GMVFSHSDPSRSPYYNIELKEIHVAGKPLKLNPRTFDGKYGAILDSGTTYAYFPEKAYYA 360

Query: 361 FKDAIMKKISFLKRINGPDPNFNDICFSGAGRDVNELSKVFPEVDMVFANGQKISLSPEN 420
           FKDAIMKKISFLK+INGPDPNF DICFSGAGRDV EL KVFPEVDMVFA+GQKISLSPEN
Sbjct: 361 FKDAIMKKISFLKQINGPDPNFKDICFSGAGRDVTELPKVFPEVDMVFADGQKISLSPEN 413

Query: 421 YLFRHTKVSGAYCLGIFKNGNDQTTLLGGM 451
           YLFRHTKVSGAYCLGIFKNGNDQTTLLGG+
Sbjct: 421 YLFRHTKVSGAYCLGIFKNGNDQTTLLGGI 413

BLAST of Spg016134 vs. ExPASy TrEMBL
Match: A0A6J1IH74 (aspartic proteinase-like protein 2 OS=Cucurbita maxima OX=3661 GN=LOC111477188 PE=3 SV=1)

HSP 1 Score: 771.5 bits (1991), Expect = 1.9e-219
Identity = 381/450 (84.67%), Postives = 396/450 (88.00%), Query Frame = 0

Query: 1   MSPPCANSPSAIVVYCLVGFNLLATIFSSSLGSRDFDYQQRPVILPLYISSTNSTHQRVL 60
           MSPP  +  SAI VYCLVGFN L  IFSSS+ SRDFDYQQRPVILPLYIS TNSTHQRVL
Sbjct: 63  MSPPSTSLHSAIFVYCLVGFNFLGAIFSSSVDSRDFDYQQRPVILPLYISPTNSTHQRVL 122

Query: 61  DRDHRLRHLQNLDKPHSSNARMRLHDDLLTNGFVFQNFSASVSVFGLFTCSLRIGGSGRL 120
           DRDHRLRHL NL+KPHSSNARMRL+DDLLTNG                            
Sbjct: 123 DRDHRLRHLLNLEKPHSSNARMRLYDDLLTNG---------------------------- 182

Query: 121 YRYYTTRLWIGTPPQEFALIVDTGSTVTYVPCSNCVECGNHQSITTGHNDPRFQPELSST 180
             YYTTRLWIGTPPQEFALIVDTGSTVTYVPCSNCV+CGNHQ       DPRFQP+LSS+
Sbjct: 183 --YYTTRLWIGTPPQEFALIVDTGSTVTYVPCSNCVDCGNHQ-------DPRFQPKLSSS 242

Query: 181 YQPVKCNIDCNCDDNGVQCTYERRYAEMSTSSGVLAEDIMSFGKESELVPQRAVFGCETM 240
           YQPVKCNIDC+CDDNGVQCTYERRYAEMSTSSGVLAEDIMSFGKESEL+PQRAVFGCETM
Sbjct: 243 YQPVKCNIDCSCDDNGVQCTYERRYAEMSTSSGVLAEDIMSFGKESELLPQRAVFGCETM 302

Query: 241 ETGDLYTQRADGIMGLGRGTLSVMDQLVDKGVVSNSFSLCYGGMDVGGGAMVLGGISSPP 300
           ETGDLYTQRADGIMGLGRGTLSVMDQLV KG VSNSFSLCYGGMDVGGGAMVLGGISSPP
Sbjct: 303 ETGDLYTQRADGIMGLGRGTLSVMDQLVGKGAVSNSFSLCYGGMDVGGGAMVLGGISSPP 362

Query: 301 GMVFTHSNPSRSPYYNIELKEIHVAGKPLKLNPTTFDGKYGTVLDSGTTYAYFPEKAYYA 360
           GMVFTHS+ SRSPYYNIELKEIHVAGKPLKLNP TFDGKYG+VLDSGTTYAYFPEKAYYA
Sbjct: 363 GMVFTHSDTSRSPYYNIELKEIHVAGKPLKLNPKTFDGKYGSVLDSGTTYAYFPEKAYYA 422

Query: 361 FKDAIMKKISFLKRINGPDPNFNDICFSGAGRDVNELSKVFPEVDMVFANGQKISLSPEN 420
           FKDA+MKKISFLK+INGPDPNF DICFSGAGRDV+ELSKVFPEV MVFANGQKISLSPEN
Sbjct: 423 FKDALMKKISFLKQINGPDPNFKDICFSGAGRDVSELSKVFPEVAMVFANGQKISLSPEN 475

Query: 421 YLFRHTKVSGAYCLGIFKNGNDQTTLLGGM 451
           YLFRHTKVSGAYCLGIFKNGNDQTTLLGG+
Sbjct: 483 YLFRHTKVSGAYCLGIFKNGNDQTTLLGGI 475

BLAST of Spg016134 vs. ExPASy TrEMBL
Match: A0A6J1F7I1 (aspartic proteinase-like protein 2 OS=Cucurbita moschata OX=3662 GN=LOC111442809 PE=3 SV=1)

HSP 1 Score: 767.3 bits (1980), Expect = 3.7e-218
Identity = 379/450 (84.22%), Postives = 396/450 (88.00%), Query Frame = 0

Query: 1   MSPPCANSPSAIVVYCLVGFNLLATIFSSSLGSRDFDYQQRPVILPLYISSTNSTHQRVL 60
           MSPP  +  SA+ VYCLVGFNLL  IFSSS+ SRDF YQQRPVILPLYIS TNSTHQRVL
Sbjct: 63  MSPPSTSLHSAVFVYCLVGFNLLGAIFSSSVDSRDFYYQQRPVILPLYISPTNSTHQRVL 122

Query: 61  DRDHRLRHLQNLDKPHSSNARMRLHDDLLTNGFVFQNFSASVSVFGLFTCSLRIGGSGRL 120
           DRDHRLRHL NL+KPHSSNARMRL+DDLLTNG                            
Sbjct: 123 DRDHRLRHLLNLEKPHSSNARMRLYDDLLTNG---------------------------- 182

Query: 121 YRYYTTRLWIGTPPQEFALIVDTGSTVTYVPCSNCVECGNHQSITTGHNDPRFQPELSST 180
             YYTTRLWIGTPPQEFALIVDTGSTVTYVPCSNCV+CGNHQ       DPRFQP+LSS+
Sbjct: 183 --YYTTRLWIGTPPQEFALIVDTGSTVTYVPCSNCVDCGNHQ-------DPRFQPKLSSS 242

Query: 181 YQPVKCNIDCNCDDNGVQCTYERRYAEMSTSSGVLAEDIMSFGKESELVPQRAVFGCETM 240
           YQPVKCNIDC+CDDNGVQCTYERRYAEMSTSSGVLAEDIMSFGKESEL+PQRAVFGCETM
Sbjct: 243 YQPVKCNIDCSCDDNGVQCTYERRYAEMSTSSGVLAEDIMSFGKESELLPQRAVFGCETM 302

Query: 241 ETGDLYTQRADGIMGLGRGTLSVMDQLVDKGVVSNSFSLCYGGMDVGGGAMVLGGISSPP 300
           ETGDLYTQRADGIMGLGRGTLSVMDQLV KG VSNSFSLCYGGMDVGGGAMVLGGISSPP
Sbjct: 303 ETGDLYTQRADGIMGLGRGTLSVMDQLVGKGAVSNSFSLCYGGMDVGGGAMVLGGISSPP 362

Query: 301 GMVFTHSNPSRSPYYNIELKEIHVAGKPLKLNPTTFDGKYGTVLDSGTTYAYFPEKAYYA 360
           GMVFTHS+ SRSPYYNIELKEIHVAGKPLKLNP TFDGKYG+VLDSGTTYAYFPEKAYYA
Sbjct: 363 GMVFTHSDTSRSPYYNIELKEIHVAGKPLKLNPRTFDGKYGSVLDSGTTYAYFPEKAYYA 422

Query: 361 FKDAIMKKISFLKRINGPDPNFNDICFSGAGRDVNELSKVFPEVDMVFANGQKISLSPEN 420
           FKDA+MKKISFLK+I+GPDPNF DICFSGAGRDV+ELSKVFPEV MVFANGQKISLSPEN
Sbjct: 423 FKDALMKKISFLKQISGPDPNFKDICFSGAGRDVSELSKVFPEVAMVFANGQKISLSPEN 475

Query: 421 YLFRHTKVSGAYCLGIFKNGNDQTTLLGGM 451
           YLFRHTKVSGAYCLGIFKNGNDQTTLLGG+
Sbjct: 483 YLFRHTKVSGAYCLGIFKNGNDQTTLLGGI 475

BLAST of Spg016134 vs. ExPASy TrEMBL
Match: A0A5A7VA17 (Aspartic proteinase-like protein 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold255G001390 PE=3 SV=1)

HSP 1 Score: 759.6 bits (1960), Expect = 7.7e-216
Identity = 382/478 (79.92%), Postives = 394/478 (82.43%), Query Frame = 0

Query: 1   MSPPCANSPSAIVVYCLVGFNLLATIFSSSLGSRDFDYQQRPVILPLYISSTNSTHQRVL 60
           MSP C NS SAIV  CLVGFNLL  I SSS+ SRD DYQQRPV+LPLYIS TNSTH+RV 
Sbjct: 507 MSPTCDNSYSAIVFCCLVGFNLLGMILSSSVDSRDLDYQQRPVLLPLYISPTNSTHRRVF 566

Query: 61  DRDHRLRHLQNLDKPHSSNARMRLHDDLLTNGFVFQNFSASVSVFGLFTCSLRIGGSGRL 120
           DRDHRLRHLQNL KPHSSNARMRLHDDLLTNG                            
Sbjct: 567 DRDHRLRHLQNLVKPHSSNARMRLHDDLLTNG---------------------------- 626

Query: 121 YRYYTTRLWIGTPPQEFALIVDTGSTVTYVPCSNCVECGNHQSITTGHNDPRFQPELSST 180
             YYTTRLWIGTPPQEFALIVDTGSTVTYVPCSNCVECGNHQ       DPRFQPELSST
Sbjct: 627 --YYTTRLWIGTPPQEFALIVDTGSTVTYVPCSNCVECGNHQ-------DPRFQPELSST 686

Query: 181 YQPVKCNIDCNCDDNGVQCTYERRYAEMSTSSGVLAEDIMSFGKESELVPQRAVFGCETM 240
           YQPVKCN+DCNCD+NGVQCTYERRYAEMSTSSGVLAED+MSFGKESELVPQRAVFGCETM
Sbjct: 687 YQPVKCNVDCNCDENGVQCTYERRYAEMSTSSGVLAEDVMSFGKESELVPQRAVFGCETM 746

Query: 241 ETGDLYTQRADGIMGLGRGTLSVMDQLVDKGVVSNSFSLCYGGMDVGGGAMVLGGISSPP 300
           ETGDLYTQRADGIMGLGRGTLSVMDQLV KGVVSNSFSLCYGGMDVGGGAMVLGGISSPP
Sbjct: 747 ETGDLYTQRADGIMGLGRGTLSVMDQLVGKGVVSNSFSLCYGGMDVGGGAMVLGGISSPP 806

Query: 301 GMVFTHSNPSRSPYYNIELKEIHVAGKPLKLNPTTFDGKYGTVLDSGTTYAYFPEKAYYA 360
           GMVF+HS+PSRSPYYNIELKEIHVAGKPLKLNP TFDGKYG +LDSGTTYAYFPEKAYYA
Sbjct: 807 GMVFSHSDPSRSPYYNIELKEIHVAGKPLKLNPRTFDGKYGAILDSGTTYAYFPEKAYYA 866

Query: 361 FKDAIMKKISFLKRINGPDPNFNDICFSGAG----------------------------R 420
           FKDAIMKKISFLK+INGPDPNF DICFSGAG                            R
Sbjct: 867 FKDAIMKKISFLKQINGPDPNFKDICFSGAGRYFYFLLGASYFRFILSGLIVEHLFFCAR 926

Query: 421 DVNELSKVFPEVDMVFANGQKISLSPENYLFRHTKVSGAYCLGIFKNGNDQTTLLGGM 451
           DV EL KVFPEVDMVFA+GQKISLSPENYLFRHTKVSGAYCLGIFKNGNDQTTLLGG+
Sbjct: 927 DVTELPKVFPEVDMVFADGQKISLSPENYLFRHTKVSGAYCLGIFKNGNDQTTLLGGI 947

BLAST of Spg016134 vs. TAIR 10
Match: AT5G43100.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 531.9 bits (1369), Expect = 5.0e-151
Identity = 266/411 (64.72%), Postives = 310/411 (75.43%), Query Frame = 0

Query: 40  QRPVILPLYISSTNSTHQRVLDRDHRLRHLQNLDKPHSSNARMRLHDDLLTNGFVFQNFS 99
           + P+I PL   S +S   R    D R R L     P   NA M+L+DDLL+NG       
Sbjct: 28  ESPMIFPL---SYSSLPPRPRVEDFRRRRLHQSQLP---NAHMKLYDDLLSNG------- 87

Query: 100 ASVSVFGLFTCSLRIGGSGRLYRYYTTRLWIGTPPQEFALIVDTGSTVTYVPCSNCVECG 159
                                  YYTTRLWIGTPPQEFALIVDTGSTVTYVPCS C +CG
Sbjct: 88  -----------------------YYTTRLWIGTPPQEFALIVDTGSTVTYVPCSTCKQCG 147

Query: 160 NHQSITTGHNDPRFQPELSSTYQPVKCNIDCNCDDNGVQCTYERRYAEMSTSSGVLAEDI 219
            HQ       DP+FQPELS++YQ +KCN DCNCDD G  C YERRYAEMS+SSGVL+ED+
Sbjct: 148 KHQ-------DPKFQPELSTSYQALKCNPDCNCDDEGKLCVYERRYAEMSSSSGVLSEDL 207

Query: 220 MSFGKESELVPQRAVFGCETMETGDLYTQRADGIMGLGRGTLSVMDQLVDKGVVSNSFSL 279
           +SFG ES+L PQRAVFGCE  ETGDL++QRADGIMGLGRG LSV+DQLVDKGV+ + FSL
Sbjct: 208 ISFGNESQLSPQRAVFGCENEETGDLFSQRADGIMGLGRGKLSVVDQLVDKGVIEDVFSL 267

Query: 280 CYGGMDVGGGAMVLGGISSPPGMVFTHSNPSRSPYYNIELKEIHVAGKPLKLNPTTFDGK 339
           CYGGM+VGGGAMVLG IS PPGMVF+HS+P RSPYYNI+LK++HVAGK LKLNP  F+GK
Sbjct: 268 CYGGMEVGGGAMVLGKISPPPGMVFSHSDPFRSPYYNIDLKQMHVAGKSLKLNPKVFNGK 327

Query: 340 YGTVLDSGTTYAYFPEKAYYAFKDAIMKKISFLKRINGPDPNFNDICFSGAGRDVNELSK 399
           +GTVLDSGTTYAYFP++A+ A KDA++K+I  LKRI+GPDPN++D+CFSGAGRDV E+  
Sbjct: 328 HGTVLDSGTTYAYFPKEAFIAIKDAVIKEIPSLKRIHGPDPNYDDVCFSGAGRDVAEIHN 387

Query: 400 VFPEVDMVFANGQKISLSPENYLFRHTKVSGAYCLGIFKNGNDQTTLLGGM 451
            FPE+ M F NGQK+ LSPENYLFRHTKV GAYCLGIF +  D TTLLGG+
Sbjct: 388 FFPEIAMEFGNGQKLILSPENYLFRHTKVRGAYCLGIFPD-RDSTTLLGGI 394

BLAST of Spg016134 vs. TAIR 10
Match: AT3G50050.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 511.1 bits (1315), Expect = 9.1e-145
Identity = 249/421 (59.14%), Postives = 307/421 (72.92%), Query Frame = 0

Query: 40  QRPVILPLYISSTNSTHQRVLDRDHRLRHLQNLDKPHSSNARMRLHDDLLTNGFVFQNFS 99
           +RP++ PL++S  NS+ + +     +L    +   PHS   RMRL+DDLL NG       
Sbjct: 42  RRPMVFPLFLSQPNSSSRSISIPHRKLHKSDSKSLPHS---RMRLYDDLLING------- 101

Query: 100 ASVSVFGLFTCSLRIGGSGRLYRYYTTRLWIGTPPQEFALIVDTGSTVTYVPCSNCVECG 159
                                  YYTTRLWIGTPPQ FALIVD+GSTVTYVPCS+C +CG
Sbjct: 102 -----------------------YYTTRLWIGTPPQMFALIVDSGSTVTYVPCSDCEQCG 161

Query: 160 NHQSITTGHNDPRFQPELSSTYQPVKCNIDCNCDDNGVQCTYERRYAEMSTSSGVLAEDI 219
            HQ       DP+FQPE+SSTYQPVKCN+DCNCDD+  QC YER YAE S+S GVL ED+
Sbjct: 162 KHQ-------DPKFQPEMSSTYQPVKCNMDCNCDDDREQCVYEREYAEHSSSKGVLGEDL 221

Query: 220 MSFGKESELVPQRAVFGCETMETGDLYTQRADGIMGLGRGTLSVMDQLVDKGVVSNSFSL 279
           +SFG ES+L PQRAVFGCET+ETGDLY+QRADGI+GLG+G LS++DQLVDKG++SNSF L
Sbjct: 222 ISFGNESQLTPQRAVFGCETVETGDLYSQRADGIIGLGQGDLSLVDQLVDKGLISNSFGL 281

Query: 280 CYGGMDVGGGAMVLGGISSPPGMVFTHSNPSRSPYYNIELKEIHVAGKPLKLNPTTFDGK 339
           CYGGMDVGGG+M+LGG   P  MVFT S+P RSPYYNI+L  I VAGK L L+   FDG+
Sbjct: 282 CYGGMDVGGGSMILGGFDYPSDMVFTDSDPDRSPYYNIDLTGIRVAGKQLSLHSRVFDGE 341

Query: 340 YGTVLDSGTTYAYFPEKAYYAFKDAIMKKISFLKRINGPDPNFNDICFS-GAGRDVNELS 399
           +G VLDSGTTYAY P+ A+ AF++A+M+++S LK+I+GPDPNF D CF   A   V+ELS
Sbjct: 342 HGAVLDSGTTYAYLPDAAFAAFEEAVMREVSTLKQIDGPDPNFKDTCFQVAASNYVSELS 401

Query: 400 KVFPEVDMVFANGQKISLSPENYLFRHTKVSGAYCLGIFKNGNDQTTLLGGMAKFPGLLI 459
           K+FP V+MVF +GQ   LSPENY+FRH+KV GAYCLG+F NG D TTLLGG+     L++
Sbjct: 402 KIFPSVEMVFKSGQSWLLSPENYMFRHSKVHGAYCLGVFPNGKDHTTLLGGIVVRNTLVV 422

BLAST of Spg016134 vs. TAIR 10
Match: AT5G22850.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 189.5 bits (480), Expect = 6.1e-48
Identity = 123/348 (35.34%), Postives = 175/348 (50.29%), Query Frame = 0

Query: 124 YTTRLWIGTPPQEFALIVDTGSTVTYVPCSNCVECGNHQSITTGHNDPRFQPELSSTYQP 183
           Y T+L +GTPP++F + VDTGS V +V C++C  C     +    N   F P  S T  P
Sbjct: 81  YYTKLRLGTPPRDFYVQVDTGSDVLWVSCASCNGCPQTSGLQIQLN--FFDPGSSVTASP 140

Query: 184 VKC----------NIDCNCDDNGVQCTYERRYAEMSTSSGVLAEDIMSFGK--ESELVPQ 243
           + C          + D  C      C Y  +Y + S +SG    D++ F     S LVP 
Sbjct: 141 ISCSDQRCSWGIQSSDSGCSVQNNLCAYTFQYGDGSGTSGFYVSDVLQFDMIVGSSLVPN 200

Query: 244 R---AVFGCETMETGDLY--TQRADGIMGLGRGTLSVMDQLVDKGVVSNSFSLCYGGMDV 303
                VFGC T +TGDL    +  DGI G G+  +SV+ QL  +G+    FS C  G + 
Sbjct: 201 STAPVVFGCSTSQTGDLVKSDRAVDGIFGFGQQGMSVISQLASQGIAPRVFSHCLKGENG 260

Query: 304 GGGAMVLGGISSPPGMVFTHSNPSRSPYYNIELKEIHVAGKPLKLNPTTF--DGKYGTVL 363
           GGG +VLG I   P MVFT   PS+ P+YN+ L  I V G+ L +NP+ F      GT++
Sbjct: 261 GGGILVLGEIVE-PNMVFTPLVPSQ-PHYNVNLLSISVNGQALPINPSVFSTSNGQGTII 320

Query: 364 DSGTTYAYFPEKAYYAFKDAIMKKISFLKRINGPDPNFNDICFSGAGRDVNELSKVFPEV 423
           D+GTT AY  E AY  F +AI   +S   R   P  +  + C+         +  +FP V
Sbjct: 321 DTGTTLAYLSEAAYVPFVEAITNAVSQSVR---PVVSKGNQCYV----ITTSVGDIFPPV 380

Query: 424 DMVFANGQKISLSPENYLFRHTKVSG--AYCLGIFKNGNDQTTLLGGM 451
            + FA G  + L+P++YL +   V G   +C+G  +  N   T+LG +
Sbjct: 381 SLNFAGGASMFLNPQDYLIQQNNVGGTAVWCIGFQRIQNQGITILGDL 417

BLAST of Spg016134 vs. TAIR 10
Match: AT1G08210.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 178.3 bits (451), Expect = 1.4e-44
Identity = 122/346 (35.26%), Postives = 180/346 (52.02%), Query Frame = 0

Query: 124 YTTRLWIGTPPQEFALIVDTGSTVTYVPCSNCVECGNHQSITTGHNDPRFQPELSSTYQP 183
           Y T++ +GTPP+EF + +DTGS V +V C++C  C     +    +   F P +SS+   
Sbjct: 84  YYTKVKLGTPPREFNVQIDTGSDVLWVSCTSCNGCPKTSELQIQLS--FFDPGVSSSASL 143

Query: 184 VKCN---------IDCNCDDNGVQCTYERRYAEMSTSSGVLAEDIMSFGK--ESELVPQR 243
           V C+          +  C  N + C+Y  +Y + S +SG    D MSF     S L    
Sbjct: 144 VSCSDRRCYSNFQTESGCSPNNL-CSYSFKYGDGSGTSGYYISDFMSFDTVITSTLAINS 203

Query: 244 A---VFGCETMETGDLYTQR--ADGIMGLGRGTLSVMDQLVDKGVVSNSFSLCYGGMDVG 303
           +   VFGC  +++GDL   R   DGI GLG+G+LSV+ QL  +G+    FS C  G   G
Sbjct: 204 SAPFVFGCSNLQSGDLQRPRRAVDGIFGLGQGSLSVISQLAVQGLAPRVFSHCLKGDKSG 263

Query: 304 GGAMVLGGISSPPGMVFTHSNPSRSPYYNIELKEIHVAGKPLKLNPTTFD--GKYGTVLD 363
           GG MVLG I   P  V+T   PS+ P+YN+ L+ I V G+ L ++P+ F      GT++D
Sbjct: 264 GGIMVLGQIKR-PDTVYTPLVPSQ-PHYNVNLQSIAVNGQILPIDPSVFTIATGDGTIID 323

Query: 364 SGTTYAYFPEKAYYAFKDAIMKKISFLKRINGPDPNFNDICFSGAGRDVNELSKVFPEVD 423
           +GTT AY P++AY  F  A+   +S   R   P    +  CF     DV+    VFP+V 
Sbjct: 324 TGTTLAYLPDEAYSPFIQAVANAVSQYGR---PITYESYQCFEITAGDVD----VFPQVS 383

Query: 424 MVFANGQKISLSPENYL-FRHTKVSGAYCLGIFKNGNDQTTLLGGM 451
           + FA G  + L P  YL    +  S  +C+G  +  + + T+LG +
Sbjct: 384 LSFAGGASMVLGPRAYLQIFSSSGSSIWCIGFQRMSHRRITILGDL 417

BLAST of Spg016134 vs. TAIR 10
Match: AT2G36670.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 169.5 bits (428), Expect = 6.5e-42
Identity = 119/348 (34.20%), Postives = 178/348 (51.15%), Query Frame = 0

Query: 124 YTTRLWIGTPPQEFALIVDTGSTVTYVPCSNCVECGNHQSITTGHNDPRFQPELSSTYQP 183
           Y T++ +G+PP EF + +DTGS + +V CS+C  C +   +  G +   F    S T   
Sbjct: 105 YFTKVKLGSPPTEFNVQIDTGSDILWVTCSSCSNCPHSSGL--GIDLHFFDAPGSLTAGS 164

Query: 184 VKCN----------IDCNCDDNGVQCTYERRYAEMSTSSGVLAEDIMSFGK---ESELVP 243
           V C+              C +N  QC Y  RY + S +SG    D   F     ES +  
Sbjct: 165 VTCSDPICSSVFQTTAAQCSENN-QCGYSFRYGDGSGTSGYYMTDTFYFDAILGESLVAN 224

Query: 244 QRA--VFGCETMETGDL--YTQRADGIMGLGRGTLSVMDQLVDKGVVSNSFSLCYGGMDV 303
             A  VFGC T ++GDL    +  DGI G G+G LSV+ QL  +G+    FS C  G   
Sbjct: 225 SSAPIVFGCSTYQSGDLTKSDKAVDGIFGFGKGKLSVVSQLSSRGITPPVFSHCLKGDGS 284

Query: 304 GGGAMVLGGISSPPGMVFTHSNPSRSPYYNIELKEIHVAGKPLKLNPTTFDGK--YGTVL 363
           GGG  VLG I   PGMV++   PS+ P+YN+ L  I V G+ L L+   F+     GT++
Sbjct: 285 GGGVFVLGEI-LVPGMVYSPLVPSQ-PHYNLNLLSIGVNGQMLPLDAAVFEASNTRGTIV 344

Query: 364 DSGTTYAYFPEKAYYAFKDAIMKKISFLKRINGPDPNFNDICFSGAGRDVNELSKVFPEV 423
           D+GTT  Y  ++AY  F +AI   +S   ++  P  +  + C+  +      +S +FP V
Sbjct: 345 DTGTTLTYLVKEAYDLFLNAISNSVS---QLVTPIISNGEQCYLVS----TSISDMFPSV 404

Query: 424 DMVFANGQKISLSPENYLFRHTKVSGA--YCLGIFKNGNDQTTLLGGM 451
            + FA G  + L P++YLF +    GA  +C+G F+   ++ T+LG +
Sbjct: 405 SLNFAGGASMMLRPQDYLFHYGIYDGASMWCIG-FQKAPEEQTILGDL 439