Spg015926 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg015926
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionRNase H domain-containing protein
Locationscaffold6: 43319407 .. 43319817 (+)
RNA-Seq ExpressionSpg015926
SyntenySpg015926
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGAGGCGGGGTGGGGTGGGTGATTCGAGACTCTACCGGATCTTTAATCGAAGCAGGTTGCAGTCGTTTTTCCCGCAATTGTCCAACCAAGGCCCTTGAAGGTGAAGCGATCCGTGTAGGTCTGAGTGCCTTTCTCAGAGGGACGAGAGAAAGTGGATGCAGACCGCCTCTGCTTGTTGAATCCGACGCCCTTAGTGTCGTGAAAGTGCTTATGGGTGAAGAAGAAGACTTCTCGGATTCGAGTTTGATTTTCGCCGAGATTTTGGAATTTGCTAAGGGAGAAAAGATCGAGTTCGATTTTTGTAGGAGGAACTCTAATTTTTTGGCCCATCAGCTTGCTCATGCAGCAACTGTTTTTGGGGATTTCTCTGTATTTTTTTGGCTCTTCTTCCCCTGTTTTGGAAGATAG

mRNA sequence

ATGAGAGGCGGGGTGGGGTGGGTGATTCGAGACTCTACCGGATCTTTAATCGAAGCAGGTTGCAGTCGTTTTTCCCGCAATTGTCCAACCAAGGCCCTTGAAGGTGAAGCGATCCGTGTAGGTCTGAGTGCCTTTCTCAGAGGGACGAGAGAAAGTGGATGCAGACCGCCTCTGCTTGTTGAATCCGACGCCCTTAGTGTCGTGAAAGTGCTTATGGGTGAAGAAGAAGACTTCTCGGATTCGAGTTTGATTTTCGCCGAGATTTTGGAATTTGCTAAGGGAGAAAAGATCGAGTTCGATTTTTGTAGGAGGAACTCTAATTTTTTGGCCCATCAGCTTGCTCATGCAGCAACTGTTTTTGGGGATTTCTCTGTATTTTTTTGGCTCTTCTTCCCCTGTTTTGGAAGATAG

Coding sequence (CDS)

ATGAGAGGCGGGGTGGGGTGGGTGATTCGAGACTCTACCGGATCTTTAATCGAAGCAGGTTGCAGTCGTTTTTCCCGCAATTGTCCAACCAAGGCCCTTGAAGGTGAAGCGATCCGTGTAGGTCTGAGTGCCTTTCTCAGAGGGACGAGAGAAAGTGGATGCAGACCGCCTCTGCTTGTTGAATCCGACGCCCTTAGTGTCGTGAAAGTGCTTATGGGTGAAGAAGAAGACTTCTCGGATTCGAGTTTGATTTTCGCCGAGATTTTGGAATTTGCTAAGGGAGAAAAGATCGAGTTCGATTTTTGTAGGAGGAACTCTAATTTTTTGGCCCATCAGCTTGCTCATGCAGCAACTGTTTTTGGGGATTTCTCTGTATTTTTTTGGCTCTTCTTCCCCTGTTTTGGAAGATAG

Protein sequence

MRGGVGWVIRDSTGSLIEAGCSRFSRNCPTKALEGEAIRVGLSAFLRGTRESGCRPPLLVESDALSVVKVLMGEEEDFSDSSLIFAEILEFAKGEKIEFDFCRRNSNFLAHQLAHAATVFGDFSVFFWLFFPCFGR
Homology
BLAST of Spg015926 vs. NCBI nr
Match: XP_021848754.1 (uncharacterized protein LOC110788421 [Spinacia oleracea])

HSP 1 Score: 64.7 bits (156), Expect = 7.1e-07
Identity = 43/130 (33.08%), Postives = 66/130 (50.77%), Query Frame = 0

Query: 2   RGGVGWVIRDSTGSLIEAGCSRFSRNCPTKALEGEAIRVGLS-AFLRGTRESGCRPPLLV 61
           R GVG V+RD+TG ++     +   NC  K  E  A   GL  AF  G R+      +++
Sbjct: 126 RVGVGMVVRDNTGDMVMGAGCKLELNCAAKEAEARAALFGLQYAFDAGYRK------VVL 185

Query: 62  ESDALSVVKVLMGEEEDFSDSSLIFAEILEFAKG-EKIEFDFCRRNSNFLAHQLAHAATV 121
           ESD  S++ +L G+  + S + +I  + L      +   F F +R+ N LAH +A A+T 
Sbjct: 186 ESDCSSLIDLLKGKASEASITQMIVRDTLSLVTNFDACTFMFAKRSCNRLAHAIAKASTA 245

Query: 122 FGDFSVFFWL 130
           F +  V  WL
Sbjct: 246 FEE--VLIWL 247

BLAST of Spg015926 vs. NCBI nr
Match: XP_022155262.1 (uncharacterized protein LOC111022403 [Momordica charantia])

HSP 1 Score: 60.8 bits (146), Expect = 1.0e-05
Identity = 43/127 (33.86%), Postives = 62/127 (48.82%), Query Frame = 0

Query: 2   RGGVGWVIRDSTGSLIEAGCSRFSRNC-PTKALEGEAIRVGLSAFLRGTRESGCRPPLLV 61
           RGG+GW+IR   G ++ AG +RF   C   K LE  AI  G    LR     G   PL +
Sbjct: 93  RGGIGWIIRSWDGDIVLAG-NRFVEACNNVKLLEASAILEG----LRNLTNLGVLRPLHI 152

Query: 62  ESDALSVVKVLMGEEEDFSDSSLIFAEILEFAKG-EKIEFDFCRRNSNFLAHQLAHAATV 121
           E+D+  V  +L  + ED + +  +  EIL      E + F    R +N  AH LA  A+V
Sbjct: 153 ETDSAEVESLLNRKREDLTKTGWVVEEILNLRDSCEILAFAKVGRETNGCAHSLAQRASV 212

Query: 122 FGDFSVF 127
             +  ++
Sbjct: 213 LRESMIW 214

BLAST of Spg015926 vs. NCBI nr
Match: ONI22498.1 (hypothetical protein PRUPE_2G133200 [Prunus persica])

HSP 1 Score: 60.8 bits (146), Expect = 1.0e-05
Identity = 42/133 (31.58%), Postives = 68/133 (51.13%), Query Frame = 0

Query: 1   MRGGVGWVIRDSTGSLIEAGCSRFSRNCPTKALEGEAIRVGLSAFLRGTRESGCRPPLLV 60
           M+GG GWVIRD  G +++AG  R  R       E +AIR  + A      + G    ++V
Sbjct: 73  MKGGYGWVIRDFVGWMLQAGGKRDRRYGSALIAEVDAIRAAVVAC-----QQGGFSHIIV 132

Query: 61  ESDALSVVKVLMGEE-EDFSDSSLIFAEILEFAKGEKIEFDFCRRNSNFLAHQLAHAATV 120
           ESD+LS ++++ G+   D     L+F       + +++ F +  R+ N  AH++A  A  
Sbjct: 133 ESDSLSAIQMVKGDRVVDAEVEGLLFDIHAVTRELQEVTFTYAPRSCNMAAHEVAAFACR 192

Query: 121 FGDFSVFFWLFFP 133
            G   +F+W F P
Sbjct: 193 NG--GLFWWDFIP 198

BLAST of Spg015926 vs. NCBI nr
Match: CAB4279811.1 (unnamed protein product [Prunus armeniaca])

HSP 1 Score: 60.1 bits (144), Expect = 1.7e-05
Identity = 40/112 (35.71%), Postives = 62/112 (55.36%), Query Frame = 0

Query: 3    GGVGWVIRDSTGSLIEAGCSRFSRNCPTKALEGEAIRVGLSAFLRGTRESGCRPPLLVES 62
            GGVGWV+RDS G +++AGC    R     A+E +AIR  L A +    +SG    L VES
Sbjct: 1082 GGVGWVVRDSYGWMVQAGCKGDMRGGSALAMEAKAIREALLACV----QSGL-SKLEVES 1141

Query: 63   DALSVVKVLMGE-EEDFSDSSLIFAEILEFAKGEKIEFDFCRRNSNFLAHQL 114
            D+L ++++L GE   D +  +++F       + +   F +  RN N  AH++
Sbjct: 1142 DSLQLIRMLGGEWNIDIAVETIVFDIKQLVCQFQHCVFLYTPRNCNKAAHKI 1188

BLAST of Spg015926 vs. NCBI nr
Match: CAB4273082.1 (unnamed protein product [Prunus armeniaca])

HSP 1 Score: 59.7 bits (143), Expect = 2.3e-05
Identity = 41/113 (36.28%), Postives = 62/113 (54.87%), Query Frame = 0

Query: 3    GGVGWVIRDSTGSLIEAGCSRFSRNCPTKALEGEAIRVGLSAFLRGTRESGCRPPLLVES 62
            GGVGWV+RDS G +++AG     R     A+E EAIR  L A +    +SG    L VES
Sbjct: 1170 GGVGWVVRDSDGWMVQAGGKGDMRGGSALAMEAEAIREALVACV----QSGL-SKLEVES 1229

Query: 63   DALSVVKVLMGE-EEDFSDSSLIFAEILEFAKGEKIEFDFCRRNSNFLAHQLA 115
            D+L V++++ GE   D +  +++F       + +   F +  RN N  AH++A
Sbjct: 1230 DSLQVIRMIGGEWNMDIAVDTIVFDIKQLVGQFQHCVFLYTPRNCNKAAHKIA 1277

BLAST of Spg015926 vs. ExPASy TrEMBL
Match: A0A6J1DNV9 (uncharacterized protein LOC111022403 OS=Momordica charantia OX=3673 GN=LOC111022403 PE=4 SV=1)

HSP 1 Score: 60.8 bits (146), Expect = 4.9e-06
Identity = 43/127 (33.86%), Postives = 62/127 (48.82%), Query Frame = 0

Query: 2   RGGVGWVIRDSTGSLIEAGCSRFSRNC-PTKALEGEAIRVGLSAFLRGTRESGCRPPLLV 61
           RGG+GW+IR   G ++ AG +RF   C   K LE  AI  G    LR     G   PL +
Sbjct: 93  RGGIGWIIRSWDGDIVLAG-NRFVEACNNVKLLEASAILEG----LRNLTNLGVLRPLHI 152

Query: 62  ESDALSVVKVLMGEEEDFSDSSLIFAEILEFAKG-EKIEFDFCRRNSNFLAHQLAHAATV 121
           E+D+  V  +L  + ED + +  +  EIL      E + F    R +N  AH LA  A+V
Sbjct: 153 ETDSAEVESLLNRKREDLTKTGWVVEEILNLRDSCEILAFAKVGRETNGCAHSLAQRASV 212

Query: 122 FGDFSVF 127
             +  ++
Sbjct: 213 LRESMIW 214

BLAST of Spg015926 vs. ExPASy TrEMBL
Match: A0A251QFD7 (RNase H domain-containing protein OS=Prunus persica OX=3760 GN=PRUPE_2G133200 PE=4 SV=1)

HSP 1 Score: 60.8 bits (146), Expect = 4.9e-06
Identity = 42/133 (31.58%), Postives = 68/133 (51.13%), Query Frame = 0

Query: 1   MRGGVGWVIRDSTGSLIEAGCSRFSRNCPTKALEGEAIRVGLSAFLRGTRESGCRPPLLV 60
           M+GG GWVIRD  G +++AG  R  R       E +AIR  + A      + G    ++V
Sbjct: 73  MKGGYGWVIRDFVGWMLQAGGKRDRRYGSALIAEVDAIRAAVVAC-----QQGGFSHIIV 132

Query: 61  ESDALSVVKVLMGEE-EDFSDSSLIFAEILEFAKGEKIEFDFCRRNSNFLAHQLAHAATV 120
           ESD+LS ++++ G+   D     L+F       + +++ F +  R+ N  AH++A  A  
Sbjct: 133 ESDSLSAIQMVKGDRVVDAEVEGLLFDIHAVTRELQEVTFTYAPRSCNMAAHEVAAFACR 192

Query: 121 FGDFSVFFWLFFP 133
            G   +F+W F P
Sbjct: 193 NG--GLFWWDFIP 198

BLAST of Spg015926 vs. ExPASy TrEMBL
Match: A0A6J5UV01 (Reverse transcriptase domain-containing protein OS=Prunus armeniaca OX=36596 GN=CURHAP_LOCUS32434 PE=4 SV=1)

HSP 1 Score: 60.1 bits (144), Expect = 8.4e-06
Identity = 40/112 (35.71%), Postives = 62/112 (55.36%), Query Frame = 0

Query: 3    GGVGWVIRDSTGSLIEAGCSRFSRNCPTKALEGEAIRVGLSAFLRGTRESGCRPPLLVES 62
            GGVGWV+RDS G +++AGC    R     A+E +AIR  L A +    +SG    L VES
Sbjct: 1082 GGVGWVVRDSYGWMVQAGCKGDMRGGSALAMEAKAIREALLACV----QSGL-SKLEVES 1141

Query: 63   DALSVVKVLMGE-EEDFSDSSLIFAEILEFAKGEKIEFDFCRRNSNFLAHQL 114
            D+L ++++L GE   D +  +++F       + +   F +  RN N  AH++
Sbjct: 1142 DSLQLIRMLGGEWNIDIAVETIVFDIKQLVCQFQHCVFLYTPRNCNKAAHKI 1188

BLAST of Spg015926 vs. ExPASy TrEMBL
Match: A0A6J5UAY2 (Reverse transcriptase domain-containing protein OS=Prunus armeniaca OX=36596 GN=CURHAP_LOCUS20161 PE=4 SV=1)

HSP 1 Score: 59.7 bits (143), Expect = 1.1e-05
Identity = 41/113 (36.28%), Postives = 62/113 (54.87%), Query Frame = 0

Query: 3    GGVGWVIRDSTGSLIEAGCSRFSRNCPTKALEGEAIRVGLSAFLRGTRESGCRPPLLVES 62
            GGVGWV+RDS G +++AG     R     A+E EAIR  L A +    +SG    L VES
Sbjct: 1170 GGVGWVVRDSDGWMVQAGGKGDMRGGSALAMEAEAIREALVACV----QSGL-SKLEVES 1229

Query: 63   DALSVVKVLMGE-EEDFSDSSLIFAEILEFAKGEKIEFDFCRRNSNFLAHQLA 115
            D+L V++++ GE   D +  +++F       + +   F +  RN N  AH++A
Sbjct: 1230 DSLQVIRMIGGEWNMDIAVDTIVFDIKQLVGQFQHCVFLYTPRNCNKAAHKIA 1277

BLAST of Spg015926 vs. ExPASy TrEMBL
Match: A2YJD5 (Reverse transcriptase domain-containing protein OS=Oryza sativa subsp. indica OX=39946 GN=OsI_25347 PE=4 SV=1)

HSP 1 Score: 57.8 bits (138), Expect = 4.2e-05
Identity = 39/132 (29.55%), Postives = 64/132 (48.48%), Query Frame = 0

Query: 3   GGVGWVIRDSTGSLIEAGCSRFSRNCPTKALEGEAIRVGLSAFLRGTRESGCRPPLLVES 62
           GG+G ++R+S G ++ A C   +R       E  A R GL+  L+ T       P+++ES
Sbjct: 724 GGIGAILRNSAGEVVFASCGSMNRCGGALEAELLACRDGLTLVLQWT-----LLPIVIES 783

Query: 63  DALSVVKVLMGEEEDFSDSSLIFAEILEFAKGEK-IEFDFCRRNSNFLAHQLAHAATVFG 122
           D L  ++++  +EE+ SD + +  EI     G + I      RN N ++H LA+      
Sbjct: 784 DCLQAIQLIQSKEEERSDLAFLIREIKHLMAGNRDIVIMKIHRNQNSVSHALAYKGC--S 843

Query: 123 DFSVFFWLFFPC 134
           +    FWL   C
Sbjct: 844 EALTAFWLDDTC 848

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_021848754.17.1e-0733.08uncharacterized protein LOC110788421 [Spinacia oleracea][more]
XP_022155262.11.0e-0533.86uncharacterized protein LOC111022403 [Momordica charantia][more]
ONI22498.11.0e-0531.58hypothetical protein PRUPE_2G133200 [Prunus persica][more]
CAB4279811.11.7e-0535.71unnamed protein product [Prunus armeniaca][more]
CAB4273082.12.3e-0536.28unnamed protein product [Prunus armeniaca][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1DNV94.9e-0633.86uncharacterized protein LOC111022403 OS=Momordica charantia OX=3673 GN=LOC111022... [more]
A0A251QFD74.9e-0631.58RNase H domain-containing protein OS=Prunus persica OX=3760 GN=PRUPE_2G133200 PE... [more]
A0A6J5UV018.4e-0635.71Reverse transcriptase domain-containing protein OS=Prunus armeniaca OX=36596 GN=... [more]
A0A6J5UAY21.1e-0536.28Reverse transcriptase domain-containing protein OS=Prunus armeniaca OX=36596 GN=... [more]
A2YJD54.2e-0529.55Reverse transcriptase domain-containing protein OS=Oryza sativa subsp. indica OX... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 2..117
e-value: 5.1E-15
score: 55.4
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 1..120
e-value: 1.9E-6
score: 29.8
NoneNo IPR availablePANTHERPTHR33033:SF67SUBFAMILY NOT NAMEDcoord: 2..121
NoneNo IPR availablePANTHERPTHR33033POLYNUCLEOTIDYL TRANSFERASE, RIBONUCLEASE H-LIKE SUPERFAMILY PROTEIN-RELATEDcoord: 2..121
IPR044730Ribonuclease H-like domain, plant typeCDDcd06222RNase_H_likecoord: 2..115
e-value: 8.63385E-13
score: 58.8648
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 3..118

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg015926.1Spg015926.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity