Spg015859 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg015859
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionNADPH-dependent diflavin oxidoreductase 1
Locationscaffold6: 42362382 .. 42364809 (-)
RNA-Seq ExpressionSpg015859
SyntenySpg015859
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAACGAAGAGGTAAAAAGGGAAAAGAGCTGAATATTGAGGCTGGAAATAACAGAATGGAAGTCAGTGGGGACCGTTAGCTGTCTGGGATCGTGGGCTCGTTCCTTGTACGTTAGATAGAGGCTGTCGAGGGGTTTATATATTGCTGGATGTGTTTGTTTAGGGTTTATGCTTAGTGTGGGCTTTTGGCTTTTGGGAGAGGTTACCAGGACTCTCGAACTTCCTGGGTGTTCTTATGTTTGGTTATTTGCTTTCTTGTTATCCTTGCTGATAGACACTACTGTTGGGCTGTTTTCACTGTTATATTTCAGTATTTGAATCAATACAGTGCTTGGTATATTGCCCTGTTCTTGTGTGTTTGGTATATTCTATCTATTTGCAGGAAAATACTTGTTAGATACCTAACATATTATTGTTTAACGGAAAACAGAGTTATAGACCTCCTCTCTGGGTCTCTGCGTGTAATGCTTTTGTTACCTTTTAGGCCTTATTTTGCTTGACTGGAGCCCCTTTTTGACTATCCTTGGGGTGTGTTTTTTTTTAGTATTCTCCATGTATTCTTTCATTTGATCTTGATGAAAGCATGACTTTTCATAAAATTGAAAAAAAGGTAAAGAGTAAACTAGCCACCAATTTCAGGAATGAGAAAGAAAATAATAAATCAAAGTGTTCACTAATATCTACATTCTCGAGTTAAATAACATCATCCAACTTCCATTTCTTTTTTTGCAACTATGTTTAAACTTAATTTTTCTTCCAGGTTATGAGTTACTATGCAACAGCTCCACATGAAAAGGAAAGACTTCAATATTTTGCCTCACCTGAAGGAAGAGATGATTTGTATCAGTACAATCAAAAAGAACGAAGAAGCGTCCTTGAGGTGATTTCAAATATTTGAATAAATGCATGCTGCAATCATTGGCAGAACTTGAAAGTTTTTGTAGGAAATTCGTTTGAATAATCATTTTGCATGCGCCAAATGTTTGTGCTGTATTTTTCTTTTAGCAATAGGCCCATTTCTGTAATTTGAAATATACCCAATAGAGAGCTAGAAGATGAAACTTTCCCATTTACCTGTCTTTGTACTGCAGGTATTAGAAGATTTTCCTTCTGTCAAAATGCCATTTGATTGGCTGGTGCAGTTGGTTCCTCCATTGAAAACAAGATCATTCTCCATATCTTCTTCCCGTCTTGCTCACCCAAATCAGGTGCACTTAACTGTAAATGTAGTGTCTTGGACGACCCCGTATAAAAGGAATCGGACGGGTCTTTGCTCAAGTTGGCTTGCTGGACTTGATCCTAAACAATGTATGTGGATCAGAAACTTAGCATCCATTATTGATGTAGTGCTAAATTTGATATATTTGGATACATTTCGTGTCATAACAGGTGTTCGTGTTCCGGTGTGGTTTCATAAGGGTTCTCTTCCCACCCCATCCCCATCTCTTCCCTTAATTCTTGTTGGACCCGGAACAGGCTGTGCACCCTTCAGAGGATTTGTAGAGGAAAGGTCCATGGAGAATTCCTCTATGTCAACTGCTCCAGTTTTGTTCTTCTTTGGTTGTCGAAACGAGGATAATGACTTTCTGTATCAAGATTTTTGGTTATCTCATTCGAAAAGTCACGGAATACTTTCAGAGGAAAAAGGTGGGGGCTTTTATATTGCCTTTTCCAGAGATCAACCGAAGAAAGTTTACGTGCAACATAAGATGCTAGAACAGAGTCAAAGGATTTGGAACATGCTTAGCGAGGGTGCTGCTGTATATGTTGCAGGCTCTTCAACAAAAATGCCCGCAGATGTATGGTCAACTTTTGAGGAAATCGTATCGAAAGAAACTCGGCTTCCACGGGAATCTGCTGTAAGGTGGCTAAGGGCATTGGAGAAGGCTGGTAAATACCATGTTGAAGCTTGGTCTTAACCTGTCTATTGTTTGATAGCTGATAATTCAACCTGGAAGAAAGGCCCACAAGATCGACTCGGAATTCATGTTTCTTCTTACAAGGGTTCTGATCATAGCGACTCGTGATTAGAGATGGCATCGTGCGGTCTAAAGCTTCTCACATTGCATGTCCTTTTTTGCATTCAAATCTTGTTATATTTTTTATCTCTCTTAGTCTCGTTCCCGTTTGATTATTCACTTTGCAAATGGTCACATCATGATCATGAGGAAATTTATTGACTAGGGATTGTAGTGTAGACATTATTATTTCGAGTACGTTATCTTTTGTTCTAGTTGGAAACTTTCATTTATTTGTATTTAAGTGTTACTGGATTGTTAAATGGGTCAGAATTATTTTCAGGTTGAACATTTCAGAGTGAAAGAAAGAAATATGCTTTCTTCATATTGCAAAGCATCCTATAACTTGCCAACAAGAGCATAGCTTAATTGGCATAAAGATGTACTGATAACCACGAGGTCTGTGATTC

mRNA sequence

ATGAACGAAGAGGTTATGAGTTACTATGCAACAGCTCCACATGAAAAGGAAAGACTTCAATATTTTGCCTCACCTGAAGGAAGAGATGATTTGTATCAGTACAATCAAAAAGAACGAAGAAGCGTCCTTGAGGTATTAGAAGATTTTCCTTCTGTCAAAATGCCATTTGATTGGCTGGTGCAGTTGGTTCCTCCATTGAAAACAAGATCATTCTCCATATCTTCTTCCCGTCTTGCTCACCCAAATCAGGTGCACTTAACTGTAAATGTAGTGTCTTGGACGACCCCGTATAAAAGGAATCGGACGGGTCTTTGCTCAAGTTGGCTTGCTGGACTTGATCCTAAACAATGTATGTGGATCAGAAACTTAGCATCCATTATTGATGTAGTGCTAAATTTGATATATTTGGATACATTTCGTGTCATAACAGGTGTTCGTGTTCCGGTGTGGTTTCATAAGGGTTCTCTTCCCACCCCATCCCCATCTCTTCCCTTAATTCTTGTTGGACCCGGAACAGGCTGTGCACCCTTCAGAGGATTTGTAGAGGAAAGGTCCATGGAGAATTCCTCTATGTCAACTGCTCCAGTTTTGTTCTTCTTTGGTTGTCGAAACGAGGATAATGACTTTCTGTATCAAGATTTTTGGTTATCTCATTCGAAAAGTCACGGAATACTTTCAGAGGAAAAAGGTGGGGGCTTTTATATTGCCTTTTCCAGAGATCAACCGAAGAAAGTTTACGTGCAACATAAGATGCTAGAACAGAGTCAAAGGATTTGGAACATGCTTAGCGAGGGTGCTGCTGTATATGTTGCAGGCTCTTCAACAAAAATGCCCGCAGATGTATGGTCAACTTTTGAGGAAATCGTATCGAAAGAAACTCGGCTTCCACGGGAATCTGCTGTAAGGTGGCTAAGGGCATTGGAGAAGGCTGGTAAATACCATGTTGAAGCTTGGTCTTAA

Coding sequence (CDS)

ATGAACGAAGAGGTTATGAGTTACTATGCAACAGCTCCACATGAAAAGGAAAGACTTCAATATTTTGCCTCACCTGAAGGAAGAGATGATTTGTATCAGTACAATCAAAAAGAACGAAGAAGCGTCCTTGAGGTATTAGAAGATTTTCCTTCTGTCAAAATGCCATTTGATTGGCTGGTGCAGTTGGTTCCTCCATTGAAAACAAGATCATTCTCCATATCTTCTTCCCGTCTTGCTCACCCAAATCAGGTGCACTTAACTGTAAATGTAGTGTCTTGGACGACCCCGTATAAAAGGAATCGGACGGGTCTTTGCTCAAGTTGGCTTGCTGGACTTGATCCTAAACAATGTATGTGGATCAGAAACTTAGCATCCATTATTGATGTAGTGCTAAATTTGATATATTTGGATACATTTCGTGTCATAACAGGTGTTCGTGTTCCGGTGTGGTTTCATAAGGGTTCTCTTCCCACCCCATCCCCATCTCTTCCCTTAATTCTTGTTGGACCCGGAACAGGCTGTGCACCCTTCAGAGGATTTGTAGAGGAAAGGTCCATGGAGAATTCCTCTATGTCAACTGCTCCAGTTTTGTTCTTCTTTGGTTGTCGAAACGAGGATAATGACTTTCTGTATCAAGATTTTTGGTTATCTCATTCGAAAAGTCACGGAATACTTTCAGAGGAAAAAGGTGGGGGCTTTTATATTGCCTTTTCCAGAGATCAACCGAAGAAAGTTTACGTGCAACATAAGATGCTAGAACAGAGTCAAAGGATTTGGAACATGCTTAGCGAGGGTGCTGCTGTATATGTTGCAGGCTCTTCAACAAAAATGCCCGCAGATGTATGGTCAACTTTTGAGGAAATCGTATCGAAAGAAACTCGGCTTCCACGGGAATCTGCTGTAAGGTGGCTAAGGGCATTGGAGAAGGCTGGTAAATACCATGTTGAAGCTTGGTCTTAA

Protein sequence

MNEEVMSYYATAPHEKERLQYFASPEGRDDLYQYNQKERRSVLEVLEDFPSVKMPFDWLVQLVPPLKTRSFSISSSRLAHPNQVHLTVNVVSWTTPYKRNRTGLCSSWLAGLDPKQCMWIRNLASIIDVVLNLIYLDTFRVITGVRVPVWFHKGSLPTPSPSLPLILVGPGTGCAPFRGFVEERSMENSSMSTAPVLFFFGCRNEDNDFLYQDFWLSHSKSHGILSEEKGGGFYIAFSRDQPKKVYVQHKMLEQSQRIWNMLSEGAAVYVAGSSTKMPADVWSTFEEIVSKETRLPRESAVRWLRALEKAGKYHVEAWS
Homology
[Close]
BLAST of Spg015859 vs. NCBI nr
Match: KAG7014852.1 (NADPH-dependent diflavin oxidoreductase 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 565.8 bits (1457), Expect = 2.3e-157
Identity = 274/316 (86.71%), Postives = 283/316 (89.56%), Query Frame = 0 

Query: 4   EVMSYYATAPHEKERLQYFASPEGRDDLYQYNQKERRSVLEVLEDFPSVKMPFDWLVQLV 63
           EVMSYYATAPHEKERLQYFASPEGRDDLYQYNQKERRSVLEVLEDFPSVKMPFDWLVQLV
Sbjct: 336 EVMSYYATAPHEKERLQYFASPEGRDDLYQYNQKERRSVLEVLEDFPSVKMPFDWLVQLV 395

Query: 64  PPLKTRSFSISSSRLAHPNQVHLTVNVVSWTTPYKRNRTGLCSSWLAGLDPKQCMWIRNL 123
           PPLKTRSFSISSS LAHPNQVHLTVNVVSWTTPYKRNR GLCSSWLAGLDP+QC      
Sbjct: 396 PPLKTRSFSISSSCLAHPNQVHLTVNVVSWTTPYKRNRLGLCSSWLAGLDPEQC------ 455

Query: 124 ASIIDVVLNLIYLDTFRVITGVRVPVWFHKGSLPTPSPSLPLILVGPGTGCAPFRGFVEE 183
                                VR+PVWFHKGSLP PSPSLPLILVGPGTGCAPFRGFVEE
Sbjct: 456 ---------------------VRIPVWFHKGSLPAPSPSLPLILVGPGTGCAPFRGFVEE 515

Query: 184 RSMENSSMSTAPVLFFFGCRNEDNDFLYQDFWLSHSKSHGILSEEKGGGFYIAFSRDQPK 243
           RSMEN SMSTAPVLFFFGCRNEDNDFLY+DFWLSHSK HG+LSEEKGGGFY+AFSRDQP+
Sbjct: 516 RSMENPSMSTAPVLFFFGCRNEDNDFLYRDFWLSHSKHHGVLSEEKGGGFYVAFSRDQPR 575

Query: 244 KVYVQHKMLEQSQRIWNMLSEGAAVYVAGSSTKMPADVWSTFEEIVSKETRLPRESAVRW 303
           KVYVQHKMLEQSQRIWNML +GAAVYVAGSSTKMPADVWSTFEEI+SKETR+PRESAVRW
Sbjct: 576 KVYVQHKMLEQSQRIWNMLKDGAAVYVAGSSTKMPADVWSTFEEIISKETRVPRESAVRW 624

Query: 304 LRALEKAGKYHVEAWS 320
           LRALEKAGKYHVEAWS
Sbjct: 636 LRALEKAGKYHVEAWS 624

[Close]
BLAST of Spg015859 vs. NCBI nr
Match: KAG6576830.1 (NADPH-dependent diflavin oxidoreductase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 565.8 bits (1457), Expect = 2.3e-157
Identity = 274/316 (86.71%), Postives = 283/316 (89.56%), Query Frame = 0 

Query: 4   EVMSYYATAPHEKERLQYFASPEGRDDLYQYNQKERRSVLEVLEDFPSVKMPFDWLVQLV 63
           EVMSYYATAPHEKERLQYFASPEGRDDLYQYNQKERRSVLEVLEDFPSVKMPFDWLVQLV
Sbjct: 628 EVMSYYATAPHEKERLQYFASPEGRDDLYQYNQKERRSVLEVLEDFPSVKMPFDWLVQLV 687

Query: 64  PPLKTRSFSISSSRLAHPNQVHLTVNVVSWTTPYKRNRTGLCSSWLAGLDPKQCMWIRNL 123
           PPLKTRSFSISSS LAHPNQVHLTVNVVSWTTPYKRNR GLCSSWLAGLDP+QC      
Sbjct: 688 PPLKTRSFSISSSCLAHPNQVHLTVNVVSWTTPYKRNRLGLCSSWLAGLDPEQC------ 747

Query: 124 ASIIDVVLNLIYLDTFRVITGVRVPVWFHKGSLPTPSPSLPLILVGPGTGCAPFRGFVEE 183
                                VR+PVWFHKGSLP PSPSLPLILVGPGTGCAPFRGFVEE
Sbjct: 748 ---------------------VRIPVWFHKGSLPAPSPSLPLILVGPGTGCAPFRGFVEE 807

Query: 184 RSMENSSMSTAPVLFFFGCRNEDNDFLYQDFWLSHSKSHGILSEEKGGGFYIAFSRDQPK 243
           RSMEN SMSTAPVLFFFGCRNEDNDFLY+DFWLSHSK HG+LSEEKGGGFY+AFSRDQP+
Sbjct: 808 RSMENPSMSTAPVLFFFGCRNEDNDFLYRDFWLSHSKHHGVLSEEKGGGFYVAFSRDQPR 867

Query: 244 KVYVQHKMLEQSQRIWNMLSEGAAVYVAGSSTKMPADVWSTFEEIVSKETRLPRESAVRW 303
           KVYVQHKMLEQSQRIWNML +GAAVYVAGSSTKMPADVWSTFEEI+SKETR+PRESAVRW
Sbjct: 868 KVYVQHKMLEQSQRIWNMLKDGAAVYVAGSSTKMPADVWSTFEEIISKETRVPRESAVRW 916

Query: 304 LRALEKAGKYHVEAWS 320
           LRALEKAGKYHVEAWS
Sbjct: 928 LRALEKAGKYHVEAWS 916

[Close]
BLAST of Spg015859 vs. NCBI nr
Match: XP_038899135.1 (NADPH-dependent diflavin oxidoreductase 1 isoform X3 [Benincasa hispida])

HSP 1 Score: 565.1 bits (1455), Expect = 3.9e-157
Identity = 273/316 (86.39%), Postives = 284/316 (89.87%), Query Frame = 0 

Query: 4   EVMSYYATAPHEKERLQYFASPEGRDDLYQYNQKERRSVLEVLEDFPSVKMPFDWLVQLV 63
           EVMSYYATAPHEKERLQYFASPEGRDDLYQYNQKERRSVLEVLEDFPSVKMPFDWLVQLV
Sbjct: 189 EVMSYYATAPHEKERLQYFASPEGRDDLYQYNQKERRSVLEVLEDFPSVKMPFDWLVQLV 248

Query: 64  PPLKTRSFSISSSRLAHPNQVHLTVNVVSWTTPYKRNRTGLCSSWLAGLDPKQCMWIRNL 123
           PPLKTRSFSISSS LAHPNQVHLTVNVVSWTTPYKRNR GLCSSWLAGLDP+QC      
Sbjct: 249 PPLKTRSFSISSSPLAHPNQVHLTVNVVSWTTPYKRNRLGLCSSWLAGLDPEQC------ 308

Query: 124 ASIIDVVLNLIYLDTFRVITGVRVPVWFHKGSLPTPSPSLPLILVGPGTGCAPFRGFVEE 183
                                VR+PVWFHKGSLP P+PSLPLILVGPGTGCAPFRGFVEE
Sbjct: 309 ---------------------VRIPVWFHKGSLPAPAPSLPLILVGPGTGCAPFRGFVEE 368

Query: 184 RSMENSSMSTAPVLFFFGCRNEDNDFLYQDFWLSHSKSHGILSEEKGGGFYIAFSRDQPK 243
           RSMEN+SM TAPVLFFFGCRN+DNDFLYQDFWLSHSK+HG+LSEEKGGGFY+AFSRDQP+
Sbjct: 369 RSMENASMPTAPVLFFFGCRNQDNDFLYQDFWLSHSKNHGVLSEEKGGGFYVAFSRDQPQ 428

Query: 244 KVYVQHKMLEQSQRIWNMLSEGAAVYVAGSSTKMPADVWSTFEEIVSKETRLPRESAVRW 303
           KVYVQHKMLEQSQRIWNML EGAAVYVAGSSTKMPADVWSTFEEI+SKET+LPRESAVRW
Sbjct: 429 KVYVQHKMLEQSQRIWNMLREGAAVYVAGSSTKMPADVWSTFEEIISKETQLPRESAVRW 477

Query: 304 LRALEKAGKYHVEAWS 320
           LRALEKAGKYHVEAWS
Sbjct: 489 LRALEKAGKYHVEAWS 477

[Close]
BLAST of Spg015859 vs. NCBI nr
Match: XP_038899117.1 (NADPH-dependent diflavin oxidoreductase 1 isoform X1 [Benincasa hispida])

HSP 1 Score: 565.1 bits (1455), Expect = 3.9e-157
Identity = 273/316 (86.39%), Postives = 284/316 (89.87%), Query Frame = 0 

Query: 4   EVMSYYATAPHEKERLQYFASPEGRDDLYQYNQKERRSVLEVLEDFPSVKMPFDWLVQLV 63
           EVMSYYATAPHEKERLQYFASPEGRDDLYQYNQKERRSVLEVLEDFPSVKMPFDWLVQLV
Sbjct: 335 EVMSYYATAPHEKERLQYFASPEGRDDLYQYNQKERRSVLEVLEDFPSVKMPFDWLVQLV 394

Query: 64  PPLKTRSFSISSSRLAHPNQVHLTVNVVSWTTPYKRNRTGLCSSWLAGLDPKQCMWIRNL 123
           PPLKTRSFSISSS LAHPNQVHLTVNVVSWTTPYKRNR GLCSSWLAGLDP+QC      
Sbjct: 395 PPLKTRSFSISSSPLAHPNQVHLTVNVVSWTTPYKRNRLGLCSSWLAGLDPEQC------ 454

Query: 124 ASIIDVVLNLIYLDTFRVITGVRVPVWFHKGSLPTPSPSLPLILVGPGTGCAPFRGFVEE 183
                                VR+PVWFHKGSLP P+PSLPLILVGPGTGCAPFRGFVEE
Sbjct: 455 ---------------------VRIPVWFHKGSLPAPAPSLPLILVGPGTGCAPFRGFVEE 514

Query: 184 RSMENSSMSTAPVLFFFGCRNEDNDFLYQDFWLSHSKSHGILSEEKGGGFYIAFSRDQPK 243
           RSMEN+SM TAPVLFFFGCRN+DNDFLYQDFWLSHSK+HG+LSEEKGGGFY+AFSRDQP+
Sbjct: 515 RSMENASMPTAPVLFFFGCRNQDNDFLYQDFWLSHSKNHGVLSEEKGGGFYVAFSRDQPQ 574

Query: 244 KVYVQHKMLEQSQRIWNMLSEGAAVYVAGSSTKMPADVWSTFEEIVSKETRLPRESAVRW 303
           KVYVQHKMLEQSQRIWNML EGAAVYVAGSSTKMPADVWSTFEEI+SKET+LPRESAVRW
Sbjct: 575 KVYVQHKMLEQSQRIWNMLREGAAVYVAGSSTKMPADVWSTFEEIISKETQLPRESAVRW 623

Query: 304 LRALEKAGKYHVEAWS 320
           LRALEKAGKYHVEAWS
Sbjct: 635 LRALEKAGKYHVEAWS 623

[Close]
BLAST of Spg015859 vs. NCBI nr
Match: XP_038899126.1 (NADPH-dependent diflavin oxidoreductase 1 isoform X2 [Benincasa hispida])

HSP 1 Score: 565.1 bits (1455), Expect = 3.9e-157
Identity = 273/316 (86.39%), Postives = 284/316 (89.87%), Query Frame = 0 

Query: 4   EVMSYYATAPHEKERLQYFASPEGRDDLYQYNQKERRSVLEVLEDFPSVKMPFDWLVQLV 63
           EVMSYYATAPHEKERLQYFASPEGRDDLYQYNQKERRSVLEVLEDFPSVKMPFDWLVQLV
Sbjct: 265 EVMSYYATAPHEKERLQYFASPEGRDDLYQYNQKERRSVLEVLEDFPSVKMPFDWLVQLV 324

Query: 64  PPLKTRSFSISSSRLAHPNQVHLTVNVVSWTTPYKRNRTGLCSSWLAGLDPKQCMWIRNL 123
           PPLKTRSFSISSS LAHPNQVHLTVNVVSWTTPYKRNR GLCSSWLAGLDP+QC      
Sbjct: 325 PPLKTRSFSISSSPLAHPNQVHLTVNVVSWTTPYKRNRLGLCSSWLAGLDPEQC------ 384

Query: 124 ASIIDVVLNLIYLDTFRVITGVRVPVWFHKGSLPTPSPSLPLILVGPGTGCAPFRGFVEE 183
                                VR+PVWFHKGSLP P+PSLPLILVGPGTGCAPFRGFVEE
Sbjct: 385 ---------------------VRIPVWFHKGSLPAPAPSLPLILVGPGTGCAPFRGFVEE 444

Query: 184 RSMENSSMSTAPVLFFFGCRNEDNDFLYQDFWLSHSKSHGILSEEKGGGFYIAFSRDQPK 243
           RSMEN+SM TAPVLFFFGCRN+DNDFLYQDFWLSHSK+HG+LSEEKGGGFY+AFSRDQP+
Sbjct: 445 RSMENASMPTAPVLFFFGCRNQDNDFLYQDFWLSHSKNHGVLSEEKGGGFYVAFSRDQPQ 504

Query: 244 KVYVQHKMLEQSQRIWNMLSEGAAVYVAGSSTKMPADVWSTFEEIVSKETRLPRESAVRW 303
           KVYVQHKMLEQSQRIWNML EGAAVYVAGSSTKMPADVWSTFEEI+SKET+LPRESAVRW
Sbjct: 505 KVYVQHKMLEQSQRIWNMLREGAAVYVAGSSTKMPADVWSTFEEIISKETQLPRESAVRW 553

Query: 304 LRALEKAGKYHVEAWS 320
           LRALEKAGKYHVEAWS
Sbjct: 565 LRALEKAGKYHVEAWS 553

[Close]
BLAST of Spg015859 vs. ExPASy Swiss-Prot
Match: Q6NPS8 (NADPH-dependent diflavin oxidoreductase 1 OS=Arabidopsis thaliana OX=3702 GN=ATR3 PE=1 SV=1)

HSP 1 Score: 447.2 bits (1149), Expect = 1.6e-124
Identity = 218/317 (68.77%), Postives = 250/317 (78.86%), Query Frame = 0 

Query: 4   EVMSYYATAPHEKERLQYFASPEGRDDLYQYNQKERRSVLEVLEDFPSVKMPFDWLVQLV 63
           E+MS+YATA HEKERLQYFASPEGRDDLY YNQKERRS+LEVLEDFPSV++PFDWLVQLV
Sbjct: 334 EIMSFYATAEHEKERLQYFASPEGRDDLYNYNQKERRSILEVLEDFPSVQIPFDWLVQLV 393

Query: 64  PPLKTRSFSISSSRLAHPNQVHLTVNVVSWTTPYKRNRTGLCSSWLAGLDPKQCMWIRNL 123
           PPLK R+FSISSS LAHP  VHLTV++VSW TPYKR R GLCSSWLA L P+Q       
Sbjct: 394 PPLKPRAFSISSSPLAHPAAVHLTVSIVSWITPYKRTRKGLCSSWLASLAPEQ------- 453

Query: 124 ASIIDVVLNLIYLDTFRVITGVRVPVWFHKGSLPTPSPSLPLILVGPGTGCAPFRGFVEE 183
                                V +PVWFHKGSLP PS SLPLILVGPGTGCAPFRGF+ E
Sbjct: 454 --------------------EVNIPVWFHKGSLPAPSQSLPLILVGPGTGCAPFRGFIAE 513

Query: 184 RSMENSSMSTAPVLFFFGCRNEDNDFLYQDFWLSHSKSHGILSEEKGGGFYIAFSRDQPK 243
           R+++  S   APV+FFFGCRN+D DFLY+DFW SH++  G+LSE KGGGFY AFSRDQPK
Sbjct: 514 RAVQAQSSPVAPVMFFFGCRNKDTDFLYRDFWESHAREGGMLSEGKGGGFYTAFSRDQPK 573

Query: 244 KVYVQHKMLEQSQRIWNMLSEGAAVYVAGSSTKMPADVWSTFEEIVSKET-RLPRESAVR 303
           KVYVQHK+ E S+R+W++L +GAAVYVAGSSTKMP DV S FE+IVS+ET    +E A R
Sbjct: 574 KVYVQHKIREMSKRVWDLLCDGAAVYVAGSSTKMPCDVMSAFEDIVSEETGGGSKEVASR 623

Query: 304 WLRALEKAGKYHVEAWS 320
           WL+ALEK G+Y+VEAWS
Sbjct: 634 WLKALEKTGRYNVEAWS 623

[Close]
BLAST of Spg015859 vs. ExPASy Swiss-Prot
Match: Q54JL0 (NADPH-dependent diflavin oxidoreductase 1 OS=Dictyostelium discoideum OX=44689 GN=redC PE=2 SV=1)

HSP 1 Score: 201.8 bits (512), Expect = 1.1e-50
Identity = 117/294 (39.80%), Postives = 182/294 (61.90%), Query Frame = 0 

Query: 4   EVMSYYATAPHEKERLQYFASPEGRDDLYQYNQKERRSVLEVLEDFPSVKMPFDWLVQLV 63
           E++S++ T P EKERL++F+S EG+DDL  YNQKE+R+ ++VL++FPS+++PF++L  L+
Sbjct: 312 ELLSHFVTNPIEKERLEFFSSTEGQDDLRTYNQKEKRNYIDVLKEFPSIEIPFEYLFDLI 371

Query: 64  PPLKTRSFSISSSRLAHPNQVHLTVNVVSWTTPYKR-NRTGLCSSWLAGLDPKQCMWIRN 123
           PP+K R FSISSS L +PN +HLTV + ++TTP++R  RTGLCS + +            
Sbjct: 372 PPIKPRPFSISSSSLLNPNTIHLTVGINTYTTPFRRLFRTGLCSQYFSSF---------- 431

Query: 124 LASIIDVVLNLIYLDTFRVITGVRVPVWFHKGSLPTPSPSLPLILVGPGTGCAPFRGFVE 183
              + D   N++ +  F   +G R+P           S  +P+I+VGPGTGCA FR F++
Sbjct: 432 ---LNDNDNNIVPI--FIKESGARLP----------KSNEIPIIMVGPGTGCAIFRSFMQ 491

Query: 184 ER----SMENSSMSTAPVLFFFGCRNEDNDFLYQDFWLSHSKSHGILSEEKGGGFYIAFS 243
           ER    +  +++      LF+FGCR+E  D+ Y+D + S+ +  GI+S+       +AFS
Sbjct: 492 ERLYFKNNSDNNNKLGDALFYFGCRSESKDYYYRDEFESNLEK-GIISK-----LSVAFS 551

Query: 244 RD--QPKKVYVQHKMLEQSQRIWNML-SEGAAVYVAGSSTKMPADVWSTFEEIV 290
           RD    KKVYVQ  +   S  IW+++ +     Y++GSS +MP DV  +   I+
Sbjct: 552 RDGKDGKKVYVQQYIENDSDLIWDIINNRNGYFYISGSSGRMPKDVKQSLLTII 574

[Close]
BLAST of Spg015859 vs. ExPASy Swiss-Prot
Match: Q9UHB4 (NADPH-dependent diflavin oxidoreductase 1 OS=Homo sapiens OX=9606 GN=NDOR1 PE=1 SV=1)

HSP 1 Score: 199.1 bits (505), Expect = 7.4e-50
Identity = 115/309 (37.22%), Postives = 170/309 (55.02%), Query Frame = 0 

Query: 15  EKERLQYFASPEGRDDLYQYNQKERRSVLEVLEDFP--SVKMPFDWLVQLVPPLKTRSFS 74
           E+E+L  F+S +G+++L++Y  + RR++LEVL DFP  +  +P D+L+ L+P ++ R+FS
Sbjct: 326 EREKLLEFSSAQGQEELFEYCNRPRRTILEVLCDFPHTAAAIPPDYLLDLIPVIRPRAFS 385

Query: 75  ISSSRLAHPNQVHLTVNVVSWTTPYKRNRTGLCSSWLAGLDPKQCMWIRNLASIIDVVLN 134
           I+SS L HP+++ + V VV + T  K  R GLCSSWLA LDP Q                
Sbjct: 386 IASSLLTHPSRLQILVAVVQFQTRLKEPRRGLCSSWLASLDPGQ---------------- 445

Query: 135 LIYLDTFRVITGVRVPVWFHKGSLPTP-SPSLPLILVGPGTGCAPFRGFVEERSMENSSM 194
                       VRVP+W   GSL  P +P  P+I+VGPGTG APFR  ++ER  +  + 
Sbjct: 446 ----------GPVRVPLWVRPGSLAFPETPDTPVIMVGPGTGVAPFRAAIQERVAQGQTG 505

Query: 195 STAPVLFFFGCRNEDNDFLYQDFWLSHSKSHGILSEEKGGGFYIAFSRDQPKKVYVQHKM 254
           +      FFGCR  D DF ++  W    K   +           AFSR+Q +KVYVQH++
Sbjct: 506 N----FLFFGCRWRDQDFYWEAEWQELEKRDCLT-------LIPAFSREQEQKVYVQHRL 565

Query: 255 LEQSQRIWNMLS-EGAAVYVAGSSTKMPADVWSTFEEIVSKETRLPRESAVRWLRALEKA 314
            E    +W +L  +GA  Y+AG++  MPADV      I  +E  L    A  +L  L++ 
Sbjct: 566 RELGSLVWELLDRQGAYFYLAGNAKSMPADVSEALMSIFQEEGGLCSPDAAAYLARLQQT 597

Query: 315 GKYHVEAWS 320
            ++  E W+
Sbjct: 626 RRFQTETWA 597

[Close]
BLAST of Spg015859 vs. ExPASy Swiss-Prot
Match: Q6PFP6 (NADPH-dependent diflavin oxidoreductase 1 OS=Danio rerio OX=7955 GN=ndor1 PE=2 SV=1)

HSP 1 Score: 198.4 bits (503), Expect = 1.3e-49
Identity = 115/320 (35.94%), Postives = 172/320 (53.75%), Query Frame = 0 

Query: 4   EVMSYYATAPHEKERLQYFASPEGRDDLYQYNQKERRSVLEVLEDFP--SVKMPFDWLVQ 63
           E+++ +AT   E+E+L  F+S  G+D L+ Y  + RR+ LEVL DFP  + ++    L+ 
Sbjct: 313 ELLATFATDELEQEKLLEFSSAAGQDTLHSYCNRPRRTALEVLTDFPHTTAELSIGRLLD 372

Query: 64  LVPPLKTRSFSISSSRLAHPNQVHLTVNVVSWTTPYKRNRTGLCSSWLAGLDPKQCMWIR 123
           L P ++ RSFSI+SS L HPN++ + + VV + T   + R GLCSSWLA LDP +     
Sbjct: 373 LFPEIQPRSFSIASSLLEHPNRIQILLAVVKYKTMLVKPRKGLCSSWLASLDPSK----- 432

Query: 124 NLASIIDVVLNLIYLDTFRVITGVRVPVWFHKGSLPTP-SPSLPLILVGPGTGCAPFRGF 183
                                  V VP+W  KGSL  P  P  P+I+VGPGTG APFR  
Sbjct: 433 ---------------------GDVYVPLWVKKGSLKFPQDPESPVIMVGPGTGVAPFRSA 492

Query: 184 VEERSMENSSMSTAPVLFFFGCRNEDNDFLYQDFWLSHSKSHGILSEEKGGGFYIAFSRD 243
           ++ER  +    +    + FFGCR+E  DF     W    ++  ++          AFSRD
Sbjct: 493 IQERVAQGKMAN----VLFFGCRSESKDFYCGSEWQEKVQAGQMI-------LVTAFSRD 552

Query: 244 QPKKVYVQHKMLEQSQRIWNMLS-EGAAVYVAGSSTKMPADVWSTFEEIVSKETRLPRES 303
           Q  KVYVQH++ EQ + +W++++ + A  Y+AG++ +MP  V    + +  KE  +    
Sbjct: 553 QEDKVYVQHRVKEQGKLLWDLIAKKNAFFYIAGNAKQMPTSVCDALKAVFQKEGGMSENQ 595

Query: 304 AVRWLRALEKAGKYHVEAWS 320
           A   L  +EK G++  E WS
Sbjct: 613 AQEMLDGMEKNGRFQSETWS 595

[Close]
BLAST of Spg015859 vs. ExPASy Swiss-Prot
Match: Q05001 (NADPH--cytochrome P450 reductase OS=Catharanthus roseus OX=4058 GN=CPR PE=2 SV=1)

HSP 1 Score: 196.8 bits (499), Expect = 3.7e-49
Identity = 112/320 (35.00%), Postives = 172/320 (53.75%), Query Frame = 0 

Query: 6   MSYYATAPHEKERLQYFASPEGRDDLYQYNQKERRSVLEVLEDFPSVKMPFD-WLVQLVP 65
           ++ YA+ P+E +RL+Y ASP G+D+  Q     +RS+LEV+ +FPS K P   +   + P
Sbjct: 428 LAAYASDPNEADRLKYLASPAGKDEYAQSLVANQRSLLEVMAEFPSAKPPLGVFFAAIAP 487

Query: 66  PLKTRSFSISSSRLAHPNQVHLTVNVVSWTTPYKRNRTGLCSSWLAGLDP----KQCMWI 125
            L+ R +SISSS    P+++H+T  +V   TP  R   G+CS+W+    P    + C W 
Sbjct: 488 RLQPRFYSISSSPRMAPSRIHVTCALVYEKTPGGRIHKGVCSTWMKNAIPLEESRDCSW- 547

Query: 126 RNLASIIDVVLNLIYLDTFRVITGVRVPVWFHKGSLPTPS-PSLPLILVGPGTGCAPFRG 185
                                      P++  + +   P+ P +P+I++GPGTG APFRG
Sbjct: 548 --------------------------APIFVRQSNFKLPADPKVPVIMIGPGTGLAPFRG 607

Query: 186 FVEER-SMENSSMSTAPVLFFFGCRNEDNDFLYQDFWLSHSKSHGILSEEKGGGFYIAFS 245
           F++ER +++         +FFFGCRN   D++Y+D  L+H    G LSE       +AFS
Sbjct: 608 FLQERLALKEEGAELGTAVFFFGCRNRKMDYIYED-ELNHFLEIGALSE-----LLVAFS 667

Query: 246 RDQPKKVYVQHKMLEQSQRIWNMLSEGAAVYVAGSSTKMPADVWSTFEEIVSKETRLPRE 305
           R+ P K YVQHKM E++  IW M+S+GA VYV G +  M  DV  T   I  ++  +   
Sbjct: 668 REGPTKQYVQHKMAEKASDIWRMISDGAYVYVCGDAKGMARDVHRTLHTIAQEQGSMDST 714

Query: 306 SAVRWLRALEKAGKYHVEAW 319
            A  +++ L+  G+Y  + W
Sbjct: 728 QAEGFVKNLQMTGRYLRDVW 714

[Close]
BLAST of Spg015859 vs. ExPASy TrEMBL
Match: A0A6J1JDP8 (NADPH-dependent diflavin oxidoreductase 1 OS=Cucurbita maxima OX=3661 GN=LOC111483472 PE=3 SV=1)

HSP 1 Score: 563.9 bits (1452), Expect = 4.2e-157
Identity = 273/316 (86.39%), Postives = 283/316 (89.56%), Query Frame = 0 

Query: 4   EVMSYYATAPHEKERLQYFASPEGRDDLYQYNQKERRSVLEVLEDFPSVKMPFDWLVQLV 63
           EVMSYYATAPHEKERLQYFASPEGRDDLYQYNQKERRSVLEVLEDFPSVKMPFDWLVQLV
Sbjct: 336 EVMSYYATAPHEKERLQYFASPEGRDDLYQYNQKERRSVLEVLEDFPSVKMPFDWLVQLV 395

Query: 64  PPLKTRSFSISSSRLAHPNQVHLTVNVVSWTTPYKRNRTGLCSSWLAGLDPKQCMWIRNL 123
           PPLK+RSFSISSS LAHPNQVHLTVNVVSWTTPYKRNR GLCSSWLAGLDP+QC      
Sbjct: 396 PPLKSRSFSISSSPLAHPNQVHLTVNVVSWTTPYKRNRLGLCSSWLAGLDPEQC------ 455

Query: 124 ASIIDVVLNLIYLDTFRVITGVRVPVWFHKGSLPTPSPSLPLILVGPGTGCAPFRGFVEE 183
                                VR+PVWFHKGSLP PSPSLPLILVGPGTGCAPFRGFVEE
Sbjct: 456 ---------------------VRIPVWFHKGSLPAPSPSLPLILVGPGTGCAPFRGFVEE 515

Query: 184 RSMENSSMSTAPVLFFFGCRNEDNDFLYQDFWLSHSKSHGILSEEKGGGFYIAFSRDQPK 243
           RSMEN SMSTAPVLFFFGCRNEDNDFLY+DFWLSHSK HG+LSEEKGGGFYIAFSRDQP+
Sbjct: 516 RSMENPSMSTAPVLFFFGCRNEDNDFLYRDFWLSHSKHHGVLSEEKGGGFYIAFSRDQPR 575

Query: 244 KVYVQHKMLEQSQRIWNMLSEGAAVYVAGSSTKMPADVWSTFEEIVSKETRLPRESAVRW 303
           KVYVQHKMLEQSQ+IWNML +GAAVYVAGSSTKMPADVWSTFEEI+SKETR+PRESAVRW
Sbjct: 576 KVYVQHKMLEQSQKIWNMLKDGAAVYVAGSSTKMPADVWSTFEEIISKETRVPRESAVRW 624

Query: 304 LRALEKAGKYHVEAWS 320
           LRALEKAGKYHVEAWS
Sbjct: 636 LRALEKAGKYHVEAWS 624

[Close]
BLAST of Spg015859 vs. ExPASy TrEMBL
Match: A0A6J1JBF2 (NADPH-dependent diflavin oxidoreductase 1 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111483472 PE=4 SV=1)

HSP 1 Score: 563.9 bits (1452), Expect = 4.2e-157
Identity = 273/316 (86.39%), Postives = 283/316 (89.56%), Query Frame = 0 

Query: 4   EVMSYYATAPHEKERLQYFASPEGRDDLYQYNQKERRSVLEVLEDFPSVKMPFDWLVQLV 63
           EVMSYYATAPHEKERLQYFASPEGRDDLYQYNQKERRSVLEVLEDFPSVKMPFDWLVQLV
Sbjct: 190 EVMSYYATAPHEKERLQYFASPEGRDDLYQYNQKERRSVLEVLEDFPSVKMPFDWLVQLV 249

Query: 64  PPLKTRSFSISSSRLAHPNQVHLTVNVVSWTTPYKRNRTGLCSSWLAGLDPKQCMWIRNL 123
           PPLK+RSFSISSS LAHPNQVHLTVNVVSWTTPYKRNR GLCSSWLAGLDP+QC      
Sbjct: 250 PPLKSRSFSISSSPLAHPNQVHLTVNVVSWTTPYKRNRLGLCSSWLAGLDPEQC------ 309

Query: 124 ASIIDVVLNLIYLDTFRVITGVRVPVWFHKGSLPTPSPSLPLILVGPGTGCAPFRGFVEE 183
                                VR+PVWFHKGSLP PSPSLPLILVGPGTGCAPFRGFVEE
Sbjct: 310 ---------------------VRIPVWFHKGSLPAPSPSLPLILVGPGTGCAPFRGFVEE 369

Query: 184 RSMENSSMSTAPVLFFFGCRNEDNDFLYQDFWLSHSKSHGILSEEKGGGFYIAFSRDQPK 243
           RSMEN SMSTAPVLFFFGCRNEDNDFLY+DFWLSHSK HG+LSEEKGGGFYIAFSRDQP+
Sbjct: 370 RSMENPSMSTAPVLFFFGCRNEDNDFLYRDFWLSHSKHHGVLSEEKGGGFYIAFSRDQPR 429

Query: 244 KVYVQHKMLEQSQRIWNMLSEGAAVYVAGSSTKMPADVWSTFEEIVSKETRLPRESAVRW 303
           KVYVQHKMLEQSQ+IWNML +GAAVYVAGSSTKMPADVWSTFEEI+SKETR+PRESAVRW
Sbjct: 430 KVYVQHKMLEQSQKIWNMLKDGAAVYVAGSSTKMPADVWSTFEEIISKETRVPRESAVRW 478

Query: 304 LRALEKAGKYHVEAWS 320
           LRALEKAGKYHVEAWS
Sbjct: 490 LRALEKAGKYHVEAWS 478

[Close]
BLAST of Spg015859 vs. ExPASy TrEMBL
Match: A0A6J1E3N5 (NADPH-dependent diflavin oxidoreductase 1 OS=Cucurbita moschata OX=3662 GN=LOC111430541 PE=3 SV=1)

HSP 1 Score: 562.0 bits (1447), Expect = 1.6e-156
Identity = 272/316 (86.08%), Postives = 282/316 (89.24%), Query Frame = 0 

Query: 4   EVMSYYATAPHEKERLQYFASPEGRDDLYQYNQKERRSVLEVLEDFPSVKMPFDWLVQLV 63
           EVMSYYATAPHEKERLQYFASPEGRDDLYQYNQKERRSVLEVLEDFPSVKMPFDWLVQLV
Sbjct: 336 EVMSYYATAPHEKERLQYFASPEGRDDLYQYNQKERRSVLEVLEDFPSVKMPFDWLVQLV 395

Query: 64  PPLKTRSFSISSSRLAHPNQVHLTVNVVSWTTPYKRNRTGLCSSWLAGLDPKQCMWIRNL 123
           PPLKTRSFSISSS LAHPNQVHLTVNVVSWTTPYKRNR GLCSSWLAGLDP+QC      
Sbjct: 396 PPLKTRSFSISSSPLAHPNQVHLTVNVVSWTTPYKRNRLGLCSSWLAGLDPEQC------ 455

Query: 124 ASIIDVVLNLIYLDTFRVITGVRVPVWFHKGSLPTPSPSLPLILVGPGTGCAPFRGFVEE 183
                                V +PVWFHKGSLP PSPSLPLILVGPGTGCAPFRGFVEE
Sbjct: 456 ---------------------VCIPVWFHKGSLPAPSPSLPLILVGPGTGCAPFRGFVEE 515

Query: 184 RSMENSSMSTAPVLFFFGCRNEDNDFLYQDFWLSHSKSHGILSEEKGGGFYIAFSRDQPK 243
           RSMEN SMSTAPVLFFFGCRNEDNDFLY+DFWLSHSK HG+LSEEKGGGFY+AFSRDQP+
Sbjct: 516 RSMENPSMSTAPVLFFFGCRNEDNDFLYRDFWLSHSKHHGVLSEEKGGGFYVAFSRDQPR 575

Query: 244 KVYVQHKMLEQSQRIWNMLSEGAAVYVAGSSTKMPADVWSTFEEIVSKETRLPRESAVRW 303
           KVYVQHKMLEQSQRIWNML +GAAVYVAGSSTKMPADVWSTFEEI+S+ETR+PRESAVRW
Sbjct: 576 KVYVQHKMLEQSQRIWNMLKDGAAVYVAGSSTKMPADVWSTFEEIISRETRVPRESAVRW 624

Query: 304 LRALEKAGKYHVEAWS 320
           LRALEKAGKYHVEAWS
Sbjct: 636 LRALEKAGKYHVEAWS 624

[Close]
BLAST of Spg015859 vs. ExPASy TrEMBL
Match: A0A6J1E3S7 (NADPH-dependent diflavin oxidoreductase 1 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111430541 PE=4 SV=1)

HSP 1 Score: 562.0 bits (1447), Expect = 1.6e-156
Identity = 272/316 (86.08%), Postives = 282/316 (89.24%), Query Frame = 0 

Query: 4   EVMSYYATAPHEKERLQYFASPEGRDDLYQYNQKERRSVLEVLEDFPSVKMPFDWLVQLV 63
           EVMSYYATAPHEKERLQYFASPEGRDDLYQYNQKERRSVLEVLEDFPSVKMPFDWLVQLV
Sbjct: 190 EVMSYYATAPHEKERLQYFASPEGRDDLYQYNQKERRSVLEVLEDFPSVKMPFDWLVQLV 249

Query: 64  PPLKTRSFSISSSRLAHPNQVHLTVNVVSWTTPYKRNRTGLCSSWLAGLDPKQCMWIRNL 123
           PPLKTRSFSISSS LAHPNQVHLTVNVVSWTTPYKRNR GLCSSWLAGLDP+QC      
Sbjct: 250 PPLKTRSFSISSSPLAHPNQVHLTVNVVSWTTPYKRNRLGLCSSWLAGLDPEQC------ 309

Query: 124 ASIIDVVLNLIYLDTFRVITGVRVPVWFHKGSLPTPSPSLPLILVGPGTGCAPFRGFVEE 183
                                V +PVWFHKGSLP PSPSLPLILVGPGTGCAPFRGFVEE
Sbjct: 310 ---------------------VCIPVWFHKGSLPAPSPSLPLILVGPGTGCAPFRGFVEE 369

Query: 184 RSMENSSMSTAPVLFFFGCRNEDNDFLYQDFWLSHSKSHGILSEEKGGGFYIAFSRDQPK 243
           RSMEN SMSTAPVLFFFGCRNEDNDFLY+DFWLSHSK HG+LSEEKGGGFY+AFSRDQP+
Sbjct: 370 RSMENPSMSTAPVLFFFGCRNEDNDFLYRDFWLSHSKHHGVLSEEKGGGFYVAFSRDQPR 429

Query: 244 KVYVQHKMLEQSQRIWNMLSEGAAVYVAGSSTKMPADVWSTFEEIVSKETRLPRESAVRW 303
           KVYVQHKMLEQSQRIWNML +GAAVYVAGSSTKMPADVWSTFEEI+S+ETR+PRESAVRW
Sbjct: 430 KVYVQHKMLEQSQRIWNMLKDGAAVYVAGSSTKMPADVWSTFEEIISRETRVPRESAVRW 478

Query: 304 LRALEKAGKYHVEAWS 320
           LRALEKAGKYHVEAWS
Sbjct: 490 LRALEKAGKYHVEAWS 478

[Close]
BLAST of Spg015859 vs. ExPASy TrEMBL
Match: A0A1S4E025 (NADPH-dependent diflavin oxidoreductase 1 isoform X3 OS=Cucumis melo OX=3656 GN=LOC103494455 PE=4 SV=1)

HSP 1 Score: 554.3 bits (1427), Expect = 3.3e-154
Identity = 266/316 (84.18%), Postives = 283/316 (89.56%), Query Frame = 0 

Query: 4   EVMSYYATAPHEKERLQYFASPEGRDDLYQYNQKERRSVLEVLEDFPSVKMPFDWLVQLV 63
           EVMSYYATAPHEKERLQYFASPEGRDDLYQYNQKERRSVLEVLEDFPSVKMPFDWLVQLV
Sbjct: 148 EVMSYYATAPHEKERLQYFASPEGRDDLYQYNQKERRSVLEVLEDFPSVKMPFDWLVQLV 207

Query: 64  PPLKTRSFSISSSRLAHPNQVHLTVNVVSWTTPYKRNRTGLCSSWLAGLDPKQCMWIRNL 123
           PPLKTRSFSISSS LAHPNQVHLTVNVVSW TPYKR R+GLCSSWLAGLDP+QC      
Sbjct: 208 PPLKTRSFSISSSALAHPNQVHLTVNVVSWMTPYKRKRSGLCSSWLAGLDPEQC------ 267

Query: 124 ASIIDVVLNLIYLDTFRVITGVRVPVWFHKGSLPTPSPSLPLILVGPGTGCAPFRGFVEE 183
                                V VPVWFHKGSLP P+PSLPLILVGPGTGCAPFRGFVEE
Sbjct: 268 ---------------------VHVPVWFHKGSLPAPAPSLPLILVGPGTGCAPFRGFVEE 327

Query: 184 RSMENSSMSTAPVLFFFGCRNEDNDFLYQDFWLSHSKSHGILSEEKGGGFYIAFSRDQPK 243
           RS+EN+SM+TAPVLFFFGCRNEDNDFLY+DFWLSHS++HG+LSEEKGGGFY+AFSRDQP+
Sbjct: 328 RSIENTSMATAPVLFFFGCRNEDNDFLYRDFWLSHSENHGVLSEEKGGGFYVAFSRDQPR 387

Query: 244 KVYVQHKMLEQSQRIWNMLSEGAAVYVAGSSTKMPADVWSTFEEIVSKETRLPRESAVRW 303
           KVYVQHKMLEQS++IWN+L EGAAVYVAGSSTKMPADVWSTFEEI+SKET+LPRESAVRW
Sbjct: 388 KVYVQHKMLEQSEKIWNLLREGAAVYVAGSSTKMPADVWSTFEEIISKETQLPRESAVRW 436

Query: 304 LRALEKAGKYHVEAWS 320
           LRALEKAGKYHVEAWS
Sbjct: 448 LRALEKAGKYHVEAWS 436

[Close]
BLAST of Spg015859 vs. TAIR 10
Match: AT3G02280.1 (Flavodoxin family protein )

HSP 1 Score: 447.2 bits (1149), Expect = 1.1e-125
Identity = 218/317 (68.77%), Postives = 250/317 (78.86%), Query Frame = 0 

Query: 4   EVMSYYATAPHEKERLQYFASPEGRDDLYQYNQKERRSVLEVLEDFPSVKMPFDWLVQLV 63
           E+MS+YATA HEKERLQYFASPEGRDDLY YNQKERRS+LEVLEDFPSV++PFDWLVQLV
Sbjct: 334 EIMSFYATAEHEKERLQYFASPEGRDDLYNYNQKERRSILEVLEDFPSVQIPFDWLVQLV 393

Query: 64  PPLKTRSFSISSSRLAHPNQVHLTVNVVSWTTPYKRNRTGLCSSWLAGLDPKQCMWIRNL 123
           PPLK R+FSISSS LAHP  VHLTV++VSW TPYKR R GLCSSWLA L P+Q       
Sbjct: 394 PPLKPRAFSISSSPLAHPAAVHLTVSIVSWITPYKRTRKGLCSSWLASLAPEQ------- 453

Query: 124 ASIIDVVLNLIYLDTFRVITGVRVPVWFHKGSLPTPSPSLPLILVGPGTGCAPFRGFVEE 183
                                V +PVWFHKGSLP PS SLPLILVGPGTGCAPFRGF+ E
Sbjct: 454 --------------------EVNIPVWFHKGSLPAPSQSLPLILVGPGTGCAPFRGFIAE 513

Query: 184 RSMENSSMSTAPVLFFFGCRNEDNDFLYQDFWLSHSKSHGILSEEKGGGFYIAFSRDQPK 243
           R+++  S   APV+FFFGCRN+D DFLY+DFW SH++  G+LSE KGGGFY AFSRDQPK
Sbjct: 514 RAVQAQSSPVAPVMFFFGCRNKDTDFLYRDFWESHAREGGMLSEGKGGGFYTAFSRDQPK 573

Query: 244 KVYVQHKMLEQSQRIWNMLSEGAAVYVAGSSTKMPADVWSTFEEIVSKET-RLPRESAVR 303
           KVYVQHK+ E S+R+W++L +GAAVYVAGSSTKMP DV S FE+IVS+ET    +E A R
Sbjct: 574 KVYVQHKIREMSKRVWDLLCDGAAVYVAGSSTKMPCDVMSAFEDIVSEETGGGSKEVASR 623

Query: 304 WLRALEKAGKYHVEAWS 320
           WL+ALEK G+Y+VEAWS
Sbjct: 634 WLKALEKTGRYNVEAWS 623

[Close]
BLAST of Spg015859 vs. TAIR 10
Match: AT4G24520.1 (P450 reductase 1 )

HSP 1 Score: 184.5 bits (467), Expect = 1.3e-46
Identity = 107/316 (33.86%), Postives = 171/316 (54.11%), Query Frame = 0 

Query: 6   MSYYATAPHEKERLQYFASPEGRDDLYQYNQKERRSVLEVLEDFPSVKMPFD-WLVQLVP 65
           ++ YAT P E E+L++  SP+G+D+  Q+    +RS+LEV+  FPS K P   +   + P
Sbjct: 406 LAAYATEPSEAEKLKHLTSPDGKDEYSQWIVASQRSLLEVMAAFPSAKPPLGVFFAAIAP 465

Query: 66  PLKTRSFSISSSRLAHPNQVHLTVNVVSWTTPYKRNRTGLCSSWLAGLDPKQCMWIRNLA 125
            L+ R +SISSS    P++VH+T  +V   TP  R   G+CS+W+               
Sbjct: 466 RLQPRYYSISSSPRLAPSRVHVTSALVYGPTPTGRIHKGVCSTWMK-------------- 525

Query: 126 SIIDVVLNLIYLDTFRVITGVRVPVWFHKGSLPTPS-PSLPLILVGPGTGCAPFRGFVEE 185
                  N +  +     +G   P++    +   PS PS P+++VGPGTG APFRGF++E
Sbjct: 526 -------NAVPAEKSHECSG--APIFIRASNFKLPSNPSTPIVMVGPGTGLAPFRGFLQE 585

Query: 186 R-SMENSSMSTAPVLFFFGCRNEDNDFLYQDFWLSHSKSHGILSEEKGGGFYIAFSRDQP 245
           R +++         L FFGCRN   DF+Y+D  L++    G++SE       +AFSR+  
Sbjct: 586 RMALKEDGEELGSSLLFFGCRNRQMDFIYED-ELNNFVDQGVISE-----LIMAFSREGA 645

Query: 246 KKVYVQHKMLEQSQRIWNMLSEGAAVYVAGSSTKMPADVWSTFEEIVSKETRLPRESAVR 305
           +K YVQHKM+E++ ++W+++ E   +YV G +  M  DV  T   IV ++  +    A  
Sbjct: 646 QKEYVQHKMMEKAAQVWDLIKEEGYLYVCGDAKGMARDVHRTLHTIVQEQEGVSSSEAEA 692

Query: 306 WLRALEKAGKYHVEAW 319
            ++ L+  G+Y  + W
Sbjct: 706 IVKKLQTEGRYLRDVW 692

[Close]
BLAST of Spg015859 vs. TAIR 10
Match: AT4G24520.2 (P450 reductase 1 )

HSP 1 Score: 184.5 bits (467), Expect = 1.3e-46
Identity = 107/316 (33.86%), Postives = 171/316 (54.11%), Query Frame = 0 

Query: 6   MSYYATAPHEKERLQYFASPEGRDDLYQYNQKERRSVLEVLEDFPSVKMPFD-WLVQLVP 65
           ++ YAT P E E+L++  SP+G+D+  Q+    +RS+LEV+  FPS K P   +   + P
Sbjct: 402 LAAYATEPSEAEKLKHLTSPDGKDEYSQWIVASQRSLLEVMAAFPSAKPPLGVFFAAIAP 461

Query: 66  PLKTRSFSISSSRLAHPNQVHLTVNVVSWTTPYKRNRTGLCSSWLAGLDPKQCMWIRNLA 125
            L+ R +SISSS    P++VH+T  +V   TP  R   G+CS+W+               
Sbjct: 462 RLQPRYYSISSSPRLAPSRVHVTSALVYGPTPTGRIHKGVCSTWMK-------------- 521

Query: 126 SIIDVVLNLIYLDTFRVITGVRVPVWFHKGSLPTPS-PSLPLILVGPGTGCAPFRGFVEE 185
                  N +  +     +G   P++    +   PS PS P+++VGPGTG APFRGF++E
Sbjct: 522 -------NAVPAEKSHECSG--APIFIRASNFKLPSNPSTPIVMVGPGTGLAPFRGFLQE 581

Query: 186 R-SMENSSMSTAPVLFFFGCRNEDNDFLYQDFWLSHSKSHGILSEEKGGGFYIAFSRDQP 245
           R +++         L FFGCRN   DF+Y+D  L++    G++SE       +AFSR+  
Sbjct: 582 RMALKEDGEELGSSLLFFGCRNRQMDFIYED-ELNNFVDQGVISE-----LIMAFSREGA 641

Query: 246 KKVYVQHKMLEQSQRIWNMLSEGAAVYVAGSSTKMPADVWSTFEEIVSKETRLPRESAVR 305
           +K YVQHKM+E++ ++W+++ E   +YV G +  M  DV  T   IV ++  +    A  
Sbjct: 642 QKEYVQHKMMEKAAQVWDLIKEEGYLYVCGDAKGMARDVHRTLHTIVQEQEGVSSSEAEA 688

Query: 306 WLRALEKAGKYHVEAW 319
            ++ L+  G+Y  + W
Sbjct: 702 IVKKLQTEGRYLRDVW 688

[Close]
BLAST of Spg015859 vs. TAIR 10
Match: AT4G30210.2 (P450 reductase 2 )

HSP 1 Score: 181.8 bits (460), Expect = 8.7e-46
Identity = 106/316 (33.54%), Postives = 173/316 (54.75%), Query Frame = 0 

Query: 6   MSYYATAPHEKERLQYFASPEGRDDLYQYNQKERRSVLEVLEDFPSVKMPFD-WLVQLVP 65
           ++ +A+ P E ERL++ ASP G+D+  ++  + +RS+LEV+ +FPS K P   +   + P
Sbjct: 425 LAAHASDPTEAERLKHLASPAGKDEYSKWVVESQRSLLEVMAEFPSAKPPLGVFFAGVAP 484

Query: 66  PLKTRSFSISSSRLAHPNQVHLTVNVVSWTTPYKRNRTGLCSSWLAGLDPKQCMWIRNLA 125
            L+ R +SISSS      ++H+T  +V    P  R   G+CS+W+    P +     N +
Sbjct: 485 RLQPRFYSISSSPKIAETRIHVTCALVYEKMPTGRIHKGVCSTWMKNAVPYEKS--ENCS 544

Query: 126 SIIDVVLNLIYLDTFRVITGVRVPVWFHKGSLPTPSPS-LPLILVGPGTGCAPFRGFVEE 185
           S                      P++  + +   PS S +P+I++GPGTG APFRGF++E
Sbjct: 545 S---------------------APIFVRQSNFKLPSDSKVPIIMIGPGTGLAPFRGFLQE 604

Query: 186 R-SMENSSMSTAPVLFFFGCRNEDNDFLYQDFWLSHSKSHGILSEEKGGGFYIAFSRDQP 245
           R ++  S +   P + FFGCRN   DF+Y++  L      G L+E       +AFSR+ P
Sbjct: 605 RLALVESGVELGPSVLFFGCRNRRMDFIYEE-ELQRFVESGALAE-----LSVAFSREGP 664

Query: 246 KKVYVQHKMLEQSQRIWNMLSEGAAVYVAGSSTKMPADVWSTFEEIVSKETRLPRESAVR 305
            K YVQHKM++++  IWNM+S+GA +YV G +  M  DV  +   I  ++  +    A  
Sbjct: 665 TKEYVQHKMMDKASDIWNMISQGAYLYVCGDAKGMARDVHRSLHTIAQEQGSMDSTKAEG 711

Query: 306 WLRALEKAGKYHVEAW 319
           +++ L+ +G+Y  + W
Sbjct: 725 FVKNLQTSGRYLRDVW 711

[Close]
BLAST of Spg015859 vs. TAIR 10
Match: AT4G30210.1 (P450 reductase 2 )

HSP 1 Score: 177.9 bits (450), Expect = 1.3e-44
Identity = 107/317 (33.75%), Postives = 173/317 (54.57%), Query Frame = 0 

Query: 6   MSYYATAPHEKERLQYFASPEGRDDLY-QYNQKERRSVLEVLEDFPSVKMPFD-WLVQLV 65
           ++ +A+ P E ERL++ ASP G+ D Y ++  + +RS+LEV+ +FPS K P   +   + 
Sbjct: 425 LAAHASDPTEAERLKHLASPAGKVDEYSKWVVESQRSLLEVMAEFPSAKPPLGVFFAGVA 484

Query: 66  PPLKTRSFSISSSRLAHPNQVHLTVNVVSWTTPYKRNRTGLCSSWLAGLDPKQCMWIRNL 125
           P L+ R +SISSS      ++H+T  +V    P  R   G+CS+W+    P +     N 
Sbjct: 485 PRLQPRFYSISSSPKIAETRIHVTCALVYEKMPTGRIHKGVCSTWMKNAVPYEKS--ENC 544

Query: 126 ASIIDVVLNLIYLDTFRVITGVRVPVWFHKGSLPTPSPS-LPLILVGPGTGCAPFRGFVE 185
           +S                      P++  + +   PS S +P+I++GPGTG APFRGF++
Sbjct: 545 SS---------------------APIFVRQSNFKLPSDSKVPIIMIGPGTGLAPFRGFLQ 604

Query: 186 ER-SMENSSMSTAPVLFFFGCRNEDNDFLYQDFWLSHSKSHGILSEEKGGGFYIAFSRDQ 245
           ER ++  S +   P + FFGCRN   DF+Y++  L      G L+E       +AFSR+ 
Sbjct: 605 ERLALVESGVELGPSVLFFGCRNRRMDFIYEE-ELQRFVESGALAE-----LSVAFSREG 664

Query: 246 PKKVYVQHKMLEQSQRIWNMLSEGAAVYVAGSSTKMPADVWSTFEEIVSKETRLPRESAV 305
           P K YVQHKM++++  IWNM+S+GA +YV G +  M  DV  +   I  ++  +    A 
Sbjct: 665 PTKEYVQHKMMDKASDIWNMISQGAYLYVCGDAKGMARDVHRSLHTIAQEQGSMDSTKAE 712

Query: 306 RWLRALEKAGKYHVEAW 319
            +++ L+ +G+Y  + W
Sbjct: 725 GFVKNLQTSGRYLRDVW 712

The following BLAST results are available for this feature:
BLAST of Spg015859 vs. NCBI nr
Match NameE-valueIdentityDescription
KAG7014852.12.3e-15786.71NADPH-dependent diflavin oxidoreductase 1 [Cucurbita argyrosperma subsp. argyros... [more]
KAG6576830.12.3e-15786.71NADPH-dependent diflavin oxidoreductase 1, partial [Cucurbita argyrosperma subsp... [more]
XP_038899135.13.9e-15786.39NADPH-dependent diflavin oxidoreductase 1 isoform X3 [Benincasa hispida][more]
XP_038899117.13.9e-15786.39NADPH-dependent diflavin oxidoreductase 1 isoform X1 [Benincasa hispida][more]
XP_038899126.13.9e-15786.39NADPH-dependent diflavin oxidoreductase 1 isoform X2 [Benincasa hispida][more]
BLAST of Spg015859 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q6NPS81.6e-12468.77NADPH-dependent diflavin oxidoreductase 1 OS=Arabidopsis thaliana OX=3702 GN=ATR... [more]
Q54JL01.1e-5039.80NADPH-dependent diflavin oxidoreductase 1 OS=Dictyostelium discoideum OX=44689 G... [more]
Q9UHB47.4e-5037.22NADPH-dependent diflavin oxidoreductase 1 OS=Homo sapiens OX=9606 GN=NDOR1 PE=1 ... [more]
Q6PFP61.3e-4935.94NADPH-dependent diflavin oxidoreductase 1 OS=Danio rerio OX=7955 GN=ndor1 PE=2 S... [more]
Q050013.7e-4935.00NADPH--cytochrome P450 reductase OS=Catharanthus roseus OX=4058 GN=CPR PE=2 SV=1[more]
BLAST of Spg015859 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1JDP84.2e-15786.39NADPH-dependent diflavin oxidoreductase 1 OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
A0A6J1JBF24.2e-15786.39NADPH-dependent diflavin oxidoreductase 1 isoform X2 OS=Cucurbita maxima OX=3661... [more]
A0A6J1E3N51.6e-15686.08NADPH-dependent diflavin oxidoreductase 1 OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
A0A6J1E3S71.6e-15686.08NADPH-dependent diflavin oxidoreductase 1 isoform X2 OS=Cucurbita moschata OX=36... [more]
A0A1S4E0253.3e-15484.18NADPH-dependent diflavin oxidoreductase 1 isoform X3 OS=Cucumis melo OX=3656 GN=... [more]
BLAST of Spg015859 vs. TAIR 10
Match NameE-valueIdentityDescription
AT3G02280.11.1e-12568.77Flavodoxin family protein [more]
AT4G24520.11.3e-4633.86P450 reductase 1 [more]
AT4G24520.21.3e-4633.86P450 reductase 1 [more]
AT4G30210.28.7e-4633.54P450 reductase 2 [more]
AT4G30210.11.3e-4433.75P450 reductase 2 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001709Flavoprotein pyridine nucleotide cytochrome reductasePRINTSPR00371FPNCRcoord: 166..185
score: 51.44
coord: 194..203
score: 31.26
coord: 244..260
score: 32.86
coord: 103..112
score: 36.78
coord: 69..76
score: 55.17
NoneNo IPR availableGENE3D2.40.30.10Translation factorscoord: 68..117
e-value: 7.9E-35
score: 122.4
NoneNo IPR availablePANTHERPTHR19384NITRIC OXIDE SYNTHASE-RELATEDcoord: 4..119
coord: 147..318
NoneNo IPR availablePANTHERPTHR19384:SF113SUBFAMILY NOT NAMEDcoord: 4..119
coord: 147..318
IPR001433Oxidoreductase FAD/NAD(P)-bindingPFAMPF00175NAD_binding_1coord: 167..278
e-value: 3.6E-12
score: 46.9
IPR039261Ferredoxin-NADP reductase (FNR), nucleotide-binding domainGENE3D3.40.50.80coord: 160..318
e-value: 1.2E-45
score: 157.1
IPR039261Ferredoxin-NADP reductase (FNR), nucleotide-binding domainSUPERFAMILY52343Ferredoxin reductase-like, C-terminal NADP-linked domaincoord: 159..318
IPR003097Sulfite reductase [NADPH] flavoprotein alpha-component-like, FAD-bindingPFAMPF00667FAD_binding_1coord: 6..108
e-value: 2.3E-28
score: 99.3
IPR023173NADPH-cytochrome p450 reductase, FAD-binding, alpha-helical domain superfamilyGENE3D1.20.990.10coord: 5..67
e-value: 7.9E-35
score: 122.4
IPR017938Riboflavin synthase-like beta-barrelSUPERFAMILY63380Riboflavin synthase domain-likecoord: 5..116

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg015859.1Spg015859.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016226 iron-sulfur cluster assembly
cellular_component GO:0005829 cytosol
molecular_function GO:0050660 flavin adenine dinucleotide binding
molecular_function GO:0010181 FMN binding
molecular_function GO:0050661 NADP binding
molecular_function GO:0003958 NADPH-hemoprotein reductase activity
molecular_function GO:0016491 oxidoreductase activity