Spg015858 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg015858
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Phospholipase D
Location	scaffold6: 42530557 .. 42531516 (-)
RNA-Seq Expression	Spg015858
Synteny	Spg015858

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTGGTCACCTTTACGAAACCTTGTCATTTGATGGGTCGAAGCATGGTCAAGGTCATCAATCTTTTCCATTTTCCTCTGCCAATGAATCGTTGAAGGTTTTGCTTTTGCATGGCTATTTGGACATTTGGGTCAAGGAAGCCAAAAACCTTCCCAATATGGACTTGTTCCGTAAGAAACTTTTAGGTAAATTGTCAAAAGTTTCCTCAAGGGCTTTCAAAAAAGTCGCCGAAAGAATGACCAGTGATCCGTACGTGACGATATCTGTATCGGGTGTTGTGATTGGAAGAACTTTCGTGATTGATAATAATGAGAATCCTGTTTGGATGCAACACTTTTATGTTCCTGTGGCTCATTATGGTGCAGAAGTGCTGTTTGTTGTCAAGGATCATGATGCCGTGGGATCAGAAACCATCGGTACTGTTGCAATCCCAGTGGAGCAATTATGTTCGGGTTCCAAGGTTGAGGGAGCCTTTCCAATCCTAGATAGTAGTAGGCAGCCTTGCAAGCCTGGGGCAGTTTTGAGTTTGTCGATTCGGTATACTCCGGTGGACCGAACAACGATTTTCGACGGCGGAATGTATGCTTCTCCTGATTACCAGGGAGTTTCTGGCACATATTTTCCTCTTAGAAAAGGGGGGAAAGTTACACTGTACCAAGACGCCCATGTCGAGGAACATGGCTGTCTTCCAACAGATTTTAGGCTTGAATATGAACACAGGAGTTGTTGGGGCGACATCTGTGAAGCAATTAGTCATGCTCGTCATTTAATCTATATTACAGGTTGGTCTGTGTACCATGAAGTTAGACTTGTTCGAGATGGCAGTAAGGCCGGAGAATGCATGCTAGGAGACCTTCTCAAAGCCAAGTCTCAGGAAGGAGTAAGAGTATTGCTTCTTGTATGGGACGATCCAACATCACATAGGATTTTGGGACATACAAAGGTATGTAGCTACTAA

mRNA sequence

ATGGCTGGTCACCTTTACGAAACCTTGTCATTTGATGGGTCGAAGCATGGTCAAGGTCATCAATCTTTTCCATTTTCCTCTGCCAATGAATCGTTGAAGGTTTTGCTTTTGCATGGCTATTTGGACATTTGGGTCAAGGAAGCCAAAAACCTTCCCAATATGGACTTGTTCCGTAAGAAACTTTTAGGTAAATTGTCAAAAGTTTCCTCAAGGGCTTTCAAAAAAGTCGCCGAAAGAATGACCAGTGATCCGTACGTGACGATATCTGTATCGGGTGTTGTGATTGGAAGAACTTTCGTGATTGATAATAATGAGAATCCTGTTTGGATGCAACACTTTTATGTTCCTGTGGCTCATTATGGTGCAGAAGTGCTGTTTGTTGTCAAGGATCATGATGCCGTGGGATCAGAAACCATCGGTACTGTTGCAATCCCAGTGGAGCAATTATGTTCGGGTTCCAAGGTTGAGGGAGCCTTTCCAATCCTAGATAGTAGTAGGCAGCCTTGCAAGCCTGGGGCAGTTTTGAGTTTGTCGATTCGGTATACTCCGGTGGACCGAACAACGATTTTCGACGGCGGAATGTATGCTTCTCCTGATTACCAGGGAGTTTCTGGCACATATTTTCCTCTTAGAAAAGGGGGGAAAGTTACACTGTACCAAGACGCCCATGTCGAGGAACATGGCTGTCTTCCAACAGATTTTAGGCTTGAATATGAACACAGGAGTTGTTGGGGCGACATCTGTGAAGCAATTAGTCATGCTCGTCATTTAATCTATATTACAGGTTGGTCTGTGTACCATGAAGTTAGACTTGTTCGAGATGGCAGTAAGGCCGGAGAATGCATGCTAGGAGACCTTCTCAAAGCCAAGTCTCAGGAAGGAGTAAGAGTATTGCTTCTTGTATGGGACGATCCAACATCACATAGGATTTTGGGACATACAAAGGTATGTAGCTACTAA

Coding sequence (CDS)

ATGGCTGGTCACCTTTACGAAACCTTGTCATTTGATGGGTCGAAGCATGGTCAAGGTCATCAATCTTTTCCATTTTCCTCTGCCAATGAATCGTTGAAGGTTTTGCTTTTGCATGGCTATTTGGACATTTGGGTCAAGGAAGCCAAAAACCTTCCCAATATGGACTTGTTCCGTAAGAAACTTTTAGGTAAATTGTCAAAAGTTTCCTCAAGGGCTTTCAAAAAAGTCGCCGAAAGAATGACCAGTGATCCGTACGTGACGATATCTGTATCGGGTGTTGTGATTGGAAGAACTTTCGTGATTGATAATAATGAGAATCCTGTTTGGATGCAACACTTTTATGTTCCTGTGGCTCATTATGGTGCAGAAGTGCTGTTTGTTGTCAAGGATCATGATGCCGTGGGATCAGAAACCATCGGTACTGTTGCAATCCCAGTGGAGCAATTATGTTCGGGTTCCAAGGTTGAGGGAGCCTTTCCAATCCTAGATAGTAGTAGGCAGCCTTGCAAGCCTGGGGCAGTTTTGAGTTTGTCGATTCGGTATACTCCGGTGGACCGAACAACGATTTTCGACGGCGGAATGTATGCTTCTCCTGATTACCAGGGAGTTTCTGGCACATATTTTCCTCTTAGAAAAGGGGGGAAAGTTACACTGTACCAAGACGCCCATGTCGAGGAACATGGCTGTCTTCCAACAGATTTTAGGCTTGAATATGAACACAGGAGTTGTTGGGGCGACATCTGTGAAGCAATTAGTCATGCTCGTCATTTAATCTATATTACAGGTTGGTCTGTGTACCATGAAGTTAGACTTGTTCGAGATGGCAGTAAGGCCGGAGAATGCATGCTAGGAGACCTTCTCAAAGCCAAGTCTCAGGAAGGAGTAAGAGTATTGCTTCTTGTATGGGACGATCCAACATCACATAGGATTTTGGGACATACAAAGGTATGTAGCTACTAA

Protein sequence

MAGHLYETLSFDGSKHGQGHQSFPFSSANESLKVLLLHGYLDIWVKEAKNLPNMDLFRKKLLGKLSKVSSRAFKKVAERMTSDPYVTISVSGVVIGRTFVIDNNENPVWMQHFYVPVAHYGAEVLFVVKDHDAVGSETIGTVAIPVEQLCSGSKVEGAFPILDSSRQPCKPGAVLSLSIRYTPVDRTTIFDGGMYASPDYQGVSGTYFPLRKGGKVTLYQDAHVEEHGCLPTDFRLEYEHRSCWGDICEAISHARHLIYITGWSVYHEVRLVRDGSKAGECMLGDLLKAKSQEGVRVLLLVWDDPTSHRILGHTKVCSY

Homology

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BLAST of Spg015858 vs. NCBI nr
Match: XP_022152769.1 (phospholipase D beta 1-like [Momordica charantia])

HSP 1 Score: 492.7 bits (1267), Expect = 2.5e-135
Identity = 245/324 (75.62%), Postives = 273/324 (84.26%), Query Frame = 0


Query: 1   MAGHLYETLSFDGSKHGQGHQSFPFSSANESLKVLLLHGYLDIWVKEAKNLPNMDLFRKK 60
           MAG ++ETLSF GS+HGQG+QS PFSSAN SLK LLLHGYLDIWVKEAKNLPN+D+F K 
Sbjct: 1   MAGRIFETLSFGGSQHGQGNQSLPFSSANASLKFLLLHGYLDIWVKEAKNLPNLDMFHKT 60

Query: 61  LLGKLSKVSSRAFKK----VAERMTSDPYVTISVSGVVIGRTFVIDNNENPVWMQHFYVP 120
           L    S+VS +  KK     +E++TSDPYVTISVSG V+GRTFVIDNNENPVWMQHF VP
Sbjct: 61  LGDMFSRVSFKGSKKSEGQKSEKVTSDPYVTISVSGAVVGRTFVIDNNENPVWMQHFNVP 120

Query: 121 VAHYGAEVLFVVKDHDAVGSETIGTVAIPVEQLCSGSKVEGAFPILDSSRQPCKPGAVLS 180
           VAHYGAEV F VKDHD VGS+ +G VAIPVEQL SG+KVEG +PIL+SSR+PCKPGAVLS
Sbjct: 121 VAHYGAEVHFAVKDHDVVGSQIMGVVAIPVEQLYSGTKVEGTYPILNSSRKPCKPGAVLS 180

Query: 181 LSIRYTPVDRTTIFDGGMYASPDYQGVSGTYFPLRKGGKVTLYQDAHVEEHGCLPTDFRL 240
           LSI+Y P D+  IF GGMYASPD+QGVSGTYFPLRKGG VTLYQDAHVE+ GCLPTDFRL
Sbjct: 181 LSIQYVPADQAAIFQGGMYASPDHQGVSGTYFPLRKGGNVTLYQDAHVED-GCLPTDFRL 240

Query: 241 E----YEHRSCWGDICEAISHARHLIYITGWSVYHEVRLVRDGSKAGECMLGDLLKAKSQ 300
                YEHRSCW DI EAIS+AR LIYITGWSVYH+V LVRDG K  EC+LGDLLKAKSQ
Sbjct: 241 HGGVGYEHRSCWHDISEAISNARRLIYITGWSVYHKVGLVRDG-KDTECILGDLLKAKSQ 300

Query: 301 EGVRVLLLVWDDPTSHRILGHTKV 317
           EGVRVLLL+WDDPTS  +LG+  V
Sbjct: 301 EGVRVLLLIWDDPTSTSMLGYKTV 322

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BLAST of Spg015858 vs. NCBI nr
Match: XP_008448951.1 (PREDICTED: phospholipase D beta 1-like [Cucumis melo] >TYK19444.1 phospholipase D beta 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 487.6 bits (1254), Expect = 7.9e-134
Identity = 241/324 (74.38%), Postives = 272/324 (83.95%), Query Frame = 0


Query: 1   MAGHLYETLSFDGSKHGQGHQSFPFSSANESLKVLLLHGYLDIWVKEAKNLPNMDLFRKK 60
           MAG ++ETLSF GS+HGQG+QS PFSS N SLK+LLLHGYLDIWVKEAKNLPN+D+F K 
Sbjct: 1   MAGRIFETLSFGGSQHGQGNQSLPFSSGNTSLKILLLHGYLDIWVKEAKNLPNLDMFHKT 60

Query: 61  LLGKLSKVSSRAFK----KVAERMTSDPYVTISVSGVVIGRTFVIDNNENPVWMQHFYVP 120
           L    SKVS +  K    +   ++TSDPYVT+SVS  VIGRTFVIDN+ENPVWMQHF VP
Sbjct: 61  LGDMFSKVSFKGSKNSDGEKPPKVTSDPYVTVSVSNAVIGRTFVIDNSENPVWMQHFNVP 120

Query: 121 VAHYGAEVLFVVKDHDAVGSETIGTVAIPVEQLCSGSKVEGAFPILDSSRQPCKPGAVLS 180
           VAHYGAEV FVVKDHD VGS+ +G VAIPVEQL SG+ VEG +PIL+SSR+PCKPGAVLS
Sbjct: 121 VAHYGAEVHFVVKDHDVVGSQIMGVVAIPVEQLYSGAIVEGTYPILNSSRKPCKPGAVLS 180

Query: 181 LSIRYTPVDRTTIFDGGMYASPDYQGVSGTYFPLRKGGKVTLYQDAHVEEHGCLPTDFRL 240
           LSI+YTP DR  IF GGMYASPD+QGV GTYFPLRKGGKVTLYQDAHVEE GCLPT+FRL
Sbjct: 181 LSIQYTPADRAAIFQGGMYASPDHQGVIGTYFPLRKGGKVTLYQDAHVEE-GCLPTEFRL 240

Query: 241 ----EYEHRSCWGDICEAISHARHLIYITGWSVYHEVRLVRDGSKAGECMLGDLLKAKSQ 300
               +YEHRSCW DI E+IS AR LIYITGWSVYH V+LVRDG++  EC+LGDLLKAKSQ
Sbjct: 241 HGGVQYEHRSCWDDISESISQARRLIYITGWSVYHSVKLVRDGTRK-ECILGDLLKAKSQ 300

Query: 301 EGVRVLLLVWDDPTSHRILGHTKV 317
           EGVRVLLL+WDDPTS  +LG+  V
Sbjct: 301 EGVRVLLLIWDDPTSTSMLGYKTV 322

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BLAST of Spg015858 vs. NCBI nr
Match: XP_011650436.1 (phospholipase D beta 1 [Cucumis sativus] >KGN55946.1 hypothetical protein Csa_011756 [Cucumis sativus])

HSP 1 Score: 484.6 bits (1246), Expect = 6.7e-133
Identity = 240/324 (74.07%), Postives = 272/324 (83.95%), Query Frame = 0


Query: 1   MAGHLYETLSFDGSKHGQGHQSFPFSSANESLKVLLLHGYLDIWVKEAKNLPNMDLFRKK 60
           MAG ++ETLSF GS+HGQG+QS PFSS N SLK+LLLHGYLDIWVKEAKNLPN+D+F K 
Sbjct: 1   MAGRIFETLSFGGSQHGQGNQSLPFSSGNTSLKILLLHGYLDIWVKEAKNLPNLDMFHKT 60

Query: 61  LLGKLSKVSSRAFK----KVAERMTSDPYVTISVSGVVIGRTFVIDNNENPVWMQHFYVP 120
           L    SKVS +  K    +  +++TSDPYVTISVS  VIGRTFVIDN+ENPVWMQHF +P
Sbjct: 61  LGDMFSKVSFKGSKNSNGEKPQKVTSDPYVTISVSNAVIGRTFVIDNSENPVWMQHFDIP 120

Query: 121 VAHYGAEVLFVVKDHDAVGSETIGTVAIPVEQLCSGSKVEGAFPILDSSRQPCKPGAVLS 180
           VAHYGAEV FVVKDHD VGS+ +G VAIPVEQL SG+ VEG +PIL+SSR+PCKPGAVLS
Sbjct: 121 VAHYGAEVHFVVKDHDVVGSQIMGVVAIPVEQLYSGAIVEGTYPILNSSRKPCKPGAVLS 180

Query: 181 LSIRYTPVDRTTIFDGGMYASPDYQGVSGTYFPLRKGGKVTLYQDAHVEEHGCLPTDFRL 240
           LSI+YTP DR  IF GGMYASPD+QGV  TYFPLRKGGKVTLYQDAHVEE GCLPTD+RL
Sbjct: 181 LSIQYTPADRAAIFRGGMYASPDHQGVLCTYFPLRKGGKVTLYQDAHVEE-GCLPTDYRL 240

Query: 241 ----EYEHRSCWGDICEAISHARHLIYITGWSVYHEVRLVRDGSKAGECMLGDLLKAKSQ 300
               +YEHRSCW DI E+IS AR LIYITGWSVYH V+LVRDG++  EC+LGDLLKAKSQ
Sbjct: 241 HGGVQYEHRSCWDDITESISQARRLIYITGWSVYHSVKLVRDGTRK-ECILGDLLKAKSQ 300

Query: 301 EGVRVLLLVWDDPTSHRILGHTKV 317
           EGVRVLLL+WDDPTS  +LG+  V
Sbjct: 301 EGVRVLLLIWDDPTSTSMLGYKTV 322

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BLAST of Spg015858 vs. NCBI nr
Match: XP_038904877.1 (phospholipase D beta 1-like [Benincasa hispida])

HSP 1 Score: 481.9 bits (1239), Expect = 4.3e-132
Identity = 240/321 (74.77%), Postives = 270/321 (84.11%), Query Frame = 0


Query: 1   MAGHLYETLSFDGSKHGQGHQSFPFSSANESLKVLLLHGYLDIWVKEAKNLPNMDLFRKK 60
           MAG + ETLSF GS+HGQG+QS PFSS N SLK+LLLHGYLDIWVK AKNLPN+D+F K 
Sbjct: 1   MAGRILETLSFGGSQHGQGNQSLPFSSGNASLKILLLHGYLDIWVKGAKNLPNLDMFHKT 60

Query: 61  LLGKLSKVSSRAFK----KVAERMTSDPYVTISVSGVVIGRTFVIDNNENPVWMQHFYVP 120
           L    SKVS +  K    +  +++TSDPYVTISVS  VIGRTFVIDN+ENPVWMQHF VP
Sbjct: 61  LGDMFSKVSFKGSKNSDGEKPQKVTSDPYVTISVSNAVIGRTFVIDNSENPVWMQHFNVP 120

Query: 121 VAHYGAEVLFVVKDHDAVGSETIGTVAIPVEQLCSGSKVEGAFPILDSSRQPCKPGAVLS 180
           VAHYGAEV FVVKDHD VGS+ +G VAIPVEQL SG+ VEG +PIL+SSR+PCKPGAVLS
Sbjct: 121 VAHYGAEVHFVVKDHDVVGSQIMGVVAIPVEQLYSGAIVEGTYPILNSSRKPCKPGAVLS 180

Query: 181 LSIRYTPVDRTTIFDGGMYASPDYQGVSGTYFPLRKGGKVTLYQDAHVEEHGCLPTDFRL 240
           LSI+YTP D+  IF GGMYASPD++GVS TYFPLRKGGKVTLYQDAHVEE GCLPTDFRL
Sbjct: 181 LSIQYTPADQAAIFHGGMYASPDHKGVSDTYFPLRKGGKVTLYQDAHVEE-GCLPTDFRL 240

Query: 241 ----EYEHRSCWGDICEAISHARHLIYITGWSVYHEVRLVRDGSKAGECMLGDLLKAKSQ 300
               +YEHRSCW DI E+IS AR LIYITGWSVYH V+LVRDG+ A EC+LGDLLKAKSQ
Sbjct: 241 HGGVQYEHRSCWDDISESISQARRLIYITGWSVYHSVKLVRDGT-AKECILGDLLKAKSQ 300

Query: 301 EGVRVLLLVWDDPTSHRILGH 314
           EGVRVLLL+WDDPTS  +LG+
Sbjct: 301 EGVRVLLLIWDDPTSTSMLGY 319

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BLAST of Spg015858 vs. NCBI nr
Match: KAG6596811.1 (Phospholipase D beta 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 477.2 bits (1227), Expect = 1.1e-130
Identity = 244/324 (75.31%), Postives = 268/324 (82.72%), Query Frame = 0


Query: 1   MAGHLYETLSFDGSKHGQGHQSFPFSSANESLKVLLLHGYLDIWVKEAKNLPNMDLFRKK 60
           MAG ++ETLSF GS+HGQ +QS PFSS N SLK LLLHGYLDI VK AKNLPNMD+F K 
Sbjct: 1   MAGRIFETLSF-GSQHGQSNQSLPFSSGNASLKFLLLHGYLDISVKSAKNLPNMDMFHKT 60

Query: 61  LLGKLSKVSSRAFK----KVAERMTSDPYVTISVSGVVIGRTFVIDNNENPVWMQHFYVP 120
           L   LSKVS ++ K    + A ++TSDPYVTISVSG VIGRTFVIDN+ENPVWMQHF VP
Sbjct: 61  LGDVLSKVSFKSSKNSDGQKAPKVTSDPYVTISVSGAVIGRTFVIDNSENPVWMQHFNVP 120

Query: 121 VAHYGAEVLFVVKDHDAVGSETIGTVAIPVEQLCSGSKVEGAFPILDSSRQPCKPGAVLS 180
           VAHYGAEV FVVKDHD VGS+ IG VAIP+EQL SG  VEG +PIL+SSR+PCKPGAVLS
Sbjct: 121 VAHYGAEVHFVVKDHDVVGSQIIGVVAIPIEQLYSGDVVEGTYPILNSSRKPCKPGAVLS 180

Query: 181 LSIRYTPVDRTTIFDGGMYASPDYQGVSGTYFPLRKGGKVTLYQDAHVEEHGCLPTDFRL 240
           LSIRY P DR  IF GGMYAS D+QGV GTYFPLRKGGKVTLYQDAHVE+ GCLP+DF+L
Sbjct: 181 LSIRYIPADRAAIFHGGMYASSDHQGVPGTYFPLRKGGKVTLYQDAHVED-GCLPSDFKL 240

Query: 241 ----EYEHRSCWGDICEAISHARHLIYITGWSVYHEVRLVRDGSKAGECMLGDLLKAKSQ 300
               EYEHRSCW DI E+IS AR LIYITGWSVYH VRLVRDG KA EC LGDLLKAKSQ
Sbjct: 241 HGGVEYEHRSCWDDITESISQARRLIYITGWSVYHNVRLVRDG-KAKECSLGDLLKAKSQ 300

Query: 301 EGVRVLLLVWDDPTSHRILGHTKV 317
           EGVRVLLL+WDDPTS  +LG+  V
Sbjct: 301 EGVRVLLLIWDDPTSTSMLGYKTV 321

[Close]

BLAST of Spg015858 vs. ExPASy Swiss-Prot
Match: P93733 (Phospholipase D beta 1 OS=Arabidopsis thaliana OX=3702 GN=PLDBETA1 PE=1 SV=4)

HSP 1 Score: 375.6 bits (963), Expect = 5.7e-103
Identity = 181/287 (63.07%), Postives = 224/287 (78.05%), Query Frame = 0


Query: 31  SLKVLLLHGYLDIWVKEAKNLPNMDLFRKKLLGKLSKVSSRAFKKVAERMTSDPYVTISV 90
           SLKVLLLHG LDIW+  AKNLPNMD+F K L     ++  +   ++  ++TSDPYV++SV
Sbjct: 265 SLKVLLLHGNLDIWIYHAKNLPNMDMFHKTLGDMFGRLPGKIEGQLTSKITSDPYVSVSV 324

Query: 91  SGVVIGRTFVIDNNENPVWMQHFYVPVAHYGAEVLFVVKDHDAVGSETIGTVAIPVEQLC 150
           +G VIGRT+V+ N+ENPVWMQHFYVPVAH+ AEV FVVKD D VGS+ IG V IPVEQ+ 
Sbjct: 325 AGAVIGRTYVMSNSENPVWMQHFYVPVAHHAAEVHFVVKDSDVVGSQLIGLVTIPVEQIY 384

Query: 151 SGSKVEGAFPILDSSRQPCKPGAVLSLSIRYTPVDRTTIFDGGMYASPDYQGVSGTYFPL 210
           SG+K+EG +PIL+S+ +PCKPGA LSLSI+YTP+D+ +++  G+ A PDYQGV GTYFPL
Sbjct: 385 SGAKIEGTYPILNSNGKPCKPGANLSLSIQYTPMDKLSVYHHGVGAGPDYQGVPGTYFPL 444

Query: 211 RKGGKVTLYQDAHVEEHGCLP---TDFRLEYEHRSCWGDICEAISHARHLIYITGWSVYH 270
           RKGG V LYQDAHV E G LP    D  + YEH  CW D+ +AI  AR LIYITGWSV+H
Sbjct: 445 RKGGTVRLYQDAHVPE-GMLPGIRLDNGMSYEHGKCWHDMFDAIRQARRLIYITGWSVWH 504

Query: 271 EVRLVRDG-SKAGECMLGDLLKAKSQEGVRVLLLVWDDPTSHRILGH 314
           +V+L+RD    A EC LG+LL++KSQEGVRVLLL+WDDPTS  ILG+
Sbjct: 505 KVKLIRDKLGPASECTLGELLRSKSQEGVRVLLLIWDDPTSRSILGY 550

[Close]

BLAST of Spg015858 vs. ExPASy Swiss-Prot
Match: Q9T053 (Phospholipase D gamma 1 OS=Arabidopsis thaliana OX=3702 GN=PLDGAMMA1 PE=1 SV=1)

HSP 1 Score: 374.8 bits (961), Expect = 9.8e-103
Identity = 188/311 (60.45%), Postives = 235/311 (75.56%), Query Frame = 0


Query: 7   ETLSF-DGSKHGQGHQSFPFSSANESLKVLLLHGYLDIWVKEAKNLPNMDLFRKKLLGKL 66
           ET+S   GS HG G Q  PF++++ SL+V LLHG LDIWVKEAK+LPNMD F  +L G L
Sbjct: 9   ETMSMGGGSSHGGGQQYVPFATSSGSLRVELLHGNLDIWVKEAKHLPNMDGFHNRLGGML 68

Query: 67  SKVSSRAFK-KVAERMTSDPYVTISVSGVVIGRTFVIDNNENPVWMQHFYVPVAHYGAEV 126
           S +  +  + + + ++TSDPYVT+S+SG VIGRTFVI N+ENPVWMQHF VPVAH  AEV
Sbjct: 69  SGLGRKKVEGEKSSKITSDPYVTVSISGAVIGRTFVISNSENPVWMQHFDVPVAHSAAEV 128

Query: 127 LFVVKDHDAVGSETIGTVAIPVEQLCSGSKVEGAFPILDSSRQPCKPGAVLSLSIRYTPV 186
            FVVKD D +GS+ +G V IP EQLCSG+++EG FPIL+SS +PCK GAVL LSI+YTP+
Sbjct: 129 HFVVKDSDIIGSQIMGAVGIPTEQLCSGNRIEGLFPILNSSGKPCKQGAVLGLSIQYTPM 188

Query: 187 DRTTIFDGGMYASPDYQGVSGTYFPLRKGGKVTLYQDAHVEEHGCLPT---DFRLEYEHR 246
           +R  ++  G+ +  +  GV GTYFPLRKGG+VTLYQDAHV++ G LP+   D  ++Y H 
Sbjct: 189 ERMRLYQMGVGSGNECVGVPGTYFPLRKGGRVTLYQDAHVDD-GTLPSVHLDGGIQYRHG 248

Query: 247 SCWGDICEAISHARHLIYITGWSVYHEVRLVRDGSKAGECMLGDLLKAKSQEGVRVLLLV 306
            CW D+ +AI  AR LIYITGWSV+H VRLVR  +   E  LG+LLK KSQEGVRVL+LV
Sbjct: 249 KCWEDMADAIRQARRLIYITGWSVFHPVRLVRRTNDPTEGTLGELLKVKSQEGVRVLVLV 308

Query: 307 WDDPTSHRILG 313
           WDDPTS  +LG
Sbjct: 309 WDDPTSRSLLG 318

[Close]

BLAST of Spg015858 vs. ExPASy Swiss-Prot
Match: O23078 (Phospholipase D beta 2 OS=Arabidopsis thaliana OX=3702 GN=PLDBETA2 PE=1 SV=3)

HSP 1 Score: 362.8 bits (930), Expect = 3.9e-99
Identity = 185/304 (60.86%), Postives = 225/304 (74.01%), Query Frame = 0


Query: 14  SKHGQGHQSFPFSSANESLKVLLLHGYLDIWVKEAKNLPNMDLFRKKLLGKLSKVSSRAF 73
           S H  G    PF  A  SLKVLLLHG LDIWV  A NLPN+DLF K L      +++   
Sbjct: 94  SPHSPGMHIVPFGKA--SLKVLLLHGNLDIWVSCANNLPNLDLFHKTLGVVFGGMTNMIE 153

Query: 74  KKVAERMTSDPYVTISVSGVVIGRTFVIDNNENPVWMQHFYVPVAHYGAEVLFVVKDHDA 133
            ++++++TSDPYV+ISV+G VIGRT+VI N+ENPVW QHFYVPVAH+ AEV FVVKD DA
Sbjct: 154 GQLSKKITSDPYVSISVAGAVIGRTYVISNSENPVWQQHFYVPVAHHAAEVHFVVKDSDA 213

Query: 134 VGSETIGTVAIPVEQLCSGSKVEGAFPILDSSRQPCKPGAVLSLSIRYTPVDRTTIFDGG 193
           VGS+ IG V IPVEQ+ SG+++EG + I DS+ +PCKPGA LSLSI+YT +++ +++  G
Sbjct: 214 VGSQLIGIVTIPVEQIYSGARIEGTYSIRDSNGKPCKPGATLSLSIQYTSMNKLSVYHSG 273

Query: 194 MYASPDYQGVSGTYFPLRKGGKVTLYQDAHVEEHGCLP---TDFRLEYEHRSCWGDICEA 253
           + A P YQGV GTYFPLR+GG VTLYQDAHV E G LP       + YEH  CW D+  A
Sbjct: 274 VGAGPYYQGVPGTYFPLREGGSVTLYQDAHVPE-GMLPGIKLGNGMCYEHGKCWHDMFHA 333

Query: 254 ISHARHLIYITGWSVYHEVRLVRD-GSKAGECMLGDLLKAKSQEGVRVLLLVWDDPTSHR 313
           I  AR LIYITGWSV+H VRLVRD    + EC LG+LL++KSQEGVRVLLLVWDDPTS  
Sbjct: 334 ICQARRLIYITGWSVWHNVRLVRDKEDPSSECRLGELLRSKSQEGVRVLLLVWDDPTSQN 393

[Close]

BLAST of Spg015858 vs. ExPASy Swiss-Prot
Match: Q9T052 (Phospholipase D gamma 3 OS=Arabidopsis thaliana OX=3702 GN=PLDGAMMA3 PE=1 SV=1)

HSP 1 Score: 356.7 bits (914), Expect = 2.8e-97
Identity = 185/318 (58.18%), Postives = 230/318 (72.33%), Query Frame = 0


Query: 7   ETLSFDG---SKHGQ--GHQSFPFSSANESLKVLLLHGYLDIWVKEAKNLPNMDLFRKKL 66
           ET+S  G   ++ GQ    Q  PF +++ SL+V LLHG LDIWVKEAK+LPNMD F   L
Sbjct: 9   ETMSMGGGSSNEFGQWLDKQLVPFDTSSGSLRVELLHGNLDIWVKEAKHLPNMDGFHNTL 68

Query: 67  LGKLSKVSSRAFKKV----AERMTSDPYVTISVSGVVIGRTFVIDNNENPVWMQHFYVPV 126
           +G +     R   KV    + ++TSDPYVT+S+SG VIGRTFVI N+ENPVWMQHF VPV
Sbjct: 69  VGGMFFGLGRRNHKVDGENSSKITSDPYVTVSISGAVIGRTFVISNSENPVWMQHFDVPV 128

Query: 127 AHYGAEVLFVVKDHDAVGSETIGTVAIPVEQLCSGSKVEGAFPILDSSRQPCKPGAVLSL 186
           AH  A+V FVVKD D +GS+ IG V IP EQLCSG+++EG FPIL+S  +PCK GAVLSL
Sbjct: 129 AHSAAKVHFVVKDSDIIGSQIIGAVEIPTEQLCSGNRIEGLFPILNSRGKPCKQGAVLSL 188

Query: 187 SIRYTPVDRTTIFDGGMYASPDYQGVSGTYFPLRKGGKVTLYQDAHVEEHGCLPT---DF 246
           SI+Y P++R  ++  G+    +  GV GTYFPLRKGG+VTLYQDAHV++ G LP+   D 
Sbjct: 189 SIQYIPMERMRLYQKGVGFGVECVGVPGTYFPLRKGGRVTLYQDAHVDD-GTLPSVHLDG 248

Query: 247 RLEYEHRSCWGDICEAISHARHLIYITGWSVYHEVRLVRDGSKAGECMLGDLLKAKSQEG 306
            ++Y H  CW D+ +AI  AR LIYITGWSV+H VRLVR  +   +  LG+LLK KSQEG
Sbjct: 249 GIQYRHGKCWEDMADAIRRARRLIYITGWSVFHPVRLVRRNNDPTQGTLGELLKVKSQEG 308

Query: 307 VRVLLLVWDDPTSHRILG 313
           VRVL+LVWDDPTS  +LG
Sbjct: 309 VRVLVLVWDDPTSRSLLG 325

[Close]

BLAST of Spg015858 vs. ExPASy Swiss-Prot
Match: Q9T051 (Phospholipase D gamma 2 OS=Arabidopsis thaliana OX=3702 GN=PLDGAMMA2 PE=1 SV=3)

HSP 1 Score: 355.5 bits (911), Expect = 6.2e-97
Identity = 182/307 (59.28%), Postives = 227/307 (73.94%), Query Frame = 0


Query: 13  GSKHGQG----HQSFPFSSANESLKVLLLHGYLDIWVKEAKNLPNMDLFRKKLLGKLS-K 72
           GS H  G     Q  P ++++ SL V LLHG LDIWVKEAK+LPNM  +R KL+G +S  
Sbjct: 6   GSNHEFGQWLDQQLVPLATSSGSLMVELLHGNLDIWVKEAKHLPNMICYRNKLVGGISFS 65

Query: 73  VSSRAFKKV----AERMTSDPYVTISVSGVVIGRTFVIDNNENPVWMQHFYVPVAHYGAE 132
              R  +KV    + + TSDPYVT+S+SG VIGRTFVI N+ENPVWMQHF VPVAH  AE
Sbjct: 66  ELGRRIRKVDGEKSSKFTSDPYVTVSISGAVIGRTFVISNSENPVWMQHFDVPVAHSAAE 125

Query: 133 VLFVVKDHDAVGSETIGTVAIPVEQLCSGSKVEGAFPILDSSRQPCKPGAVLSLSIRYTP 192
           V FVVKD+D +GS+ IG V IP +QLCSG+++EG FPIL+SS +PC+ GA+LSLSI+YTP
Sbjct: 126 VHFVVKDNDPIGSKIIGVVGIPTKQLCSGNRIEGLFPILNSSGKPCRKGAMLSLSIQYTP 185

Query: 193 VDRTTIFDGGMYASPDYQGVSGTYFPLRKGGKVTLYQDAHVEEHGCLPT---DFRLEYEH 252
           ++R  ++  G+ +  +  GV GTYFPLRKGG+VTLYQDAHV++ G LP+   D  ++Y H
Sbjct: 186 MERMRLYQKGVGSGVECVGVPGTYFPLRKGGRVTLYQDAHVDD-GTLPSVHLDGGIQYRH 245

Query: 253 RSCWGDICEAISHARHLIYITGWSVYHEVRLVRDGSKAGECMLGDLLKAKSQEGVRVLLL 308
             CW D+ +AI  AR LIYITGWSV+H VRLVR  +   E  LG+LLK KSQEGVRVL+L
Sbjct: 246 GKCWEDMADAIRRARRLIYITGWSVFHPVRLVRRNNDPTEGTLGELLKVKSQEGVRVLVL 305

[Close]

BLAST of Spg015858 vs. ExPASy TrEMBL
Match: A0A6J1DH01 (Phospholipase D OS=Momordica charantia OX=3673 GN=LOC111020410 PE=3 SV=1)

HSP 1 Score: 492.7 bits (1267), Expect = 1.2e-135
Identity = 245/324 (75.62%), Postives = 273/324 (84.26%), Query Frame = 0


Query: 1   MAGHLYETLSFDGSKHGQGHQSFPFSSANESLKVLLLHGYLDIWVKEAKNLPNMDLFRKK 60
           MAG ++ETLSF GS+HGQG+QS PFSSAN SLK LLLHGYLDIWVKEAKNLPN+D+F K 
Sbjct: 1   MAGRIFETLSFGGSQHGQGNQSLPFSSANASLKFLLLHGYLDIWVKEAKNLPNLDMFHKT 60

Query: 61  LLGKLSKVSSRAFKK----VAERMTSDPYVTISVSGVVIGRTFVIDNNENPVWMQHFYVP 120
           L    S+VS +  KK     +E++TSDPYVTISVSG V+GRTFVIDNNENPVWMQHF VP
Sbjct: 61  LGDMFSRVSFKGSKKSEGQKSEKVTSDPYVTISVSGAVVGRTFVIDNNENPVWMQHFNVP 120

Query: 121 VAHYGAEVLFVVKDHDAVGSETIGTVAIPVEQLCSGSKVEGAFPILDSSRQPCKPGAVLS 180
           VAHYGAEV F VKDHD VGS+ +G VAIPVEQL SG+KVEG +PIL+SSR+PCKPGAVLS
Sbjct: 121 VAHYGAEVHFAVKDHDVVGSQIMGVVAIPVEQLYSGTKVEGTYPILNSSRKPCKPGAVLS 180

Query: 181 LSIRYTPVDRTTIFDGGMYASPDYQGVSGTYFPLRKGGKVTLYQDAHVEEHGCLPTDFRL 240
           LSI+Y P D+  IF GGMYASPD+QGVSGTYFPLRKGG VTLYQDAHVE+ GCLPTDFRL
Sbjct: 181 LSIQYVPADQAAIFQGGMYASPDHQGVSGTYFPLRKGGNVTLYQDAHVED-GCLPTDFRL 240

Query: 241 E----YEHRSCWGDICEAISHARHLIYITGWSVYHEVRLVRDGSKAGECMLGDLLKAKSQ 300
                YEHRSCW DI EAIS+AR LIYITGWSVYH+V LVRDG K  EC+LGDLLKAKSQ
Sbjct: 241 HGGVGYEHRSCWHDISEAISNARRLIYITGWSVYHKVGLVRDG-KDTECILGDLLKAKSQ 300

Query: 301 EGVRVLLLVWDDPTSHRILGHTKV 317
           EGVRVLLL+WDDPTS  +LG+  V
Sbjct: 301 EGVRVLLLIWDDPTSTSMLGYKTV 322

[Close]

BLAST of Spg015858 vs. ExPASy TrEMBL
Match: A0A5D3D7C2 (Phospholipase D OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold443G00960 PE=3 SV=1)

HSP 1 Score: 487.6 bits (1254), Expect = 3.8e-134
Identity = 241/324 (74.38%), Postives = 272/324 (83.95%), Query Frame = 0


Query: 1   MAGHLYETLSFDGSKHGQGHQSFPFSSANESLKVLLLHGYLDIWVKEAKNLPNMDLFRKK 60
           MAG ++ETLSF GS+HGQG+QS PFSS N SLK+LLLHGYLDIWVKEAKNLPN+D+F K 
Sbjct: 1   MAGRIFETLSFGGSQHGQGNQSLPFSSGNTSLKILLLHGYLDIWVKEAKNLPNLDMFHKT 60

Query: 61  LLGKLSKVSSRAFK----KVAERMTSDPYVTISVSGVVIGRTFVIDNNENPVWMQHFYVP 120
           L    SKVS +  K    +   ++TSDPYVT+SVS  VIGRTFVIDN+ENPVWMQHF VP
Sbjct: 61  LGDMFSKVSFKGSKNSDGEKPPKVTSDPYVTVSVSNAVIGRTFVIDNSENPVWMQHFNVP 120

Query: 121 VAHYGAEVLFVVKDHDAVGSETIGTVAIPVEQLCSGSKVEGAFPILDSSRQPCKPGAVLS 180
           VAHYGAEV FVVKDHD VGS+ +G VAIPVEQL SG+ VEG +PIL+SSR+PCKPGAVLS
Sbjct: 121 VAHYGAEVHFVVKDHDVVGSQIMGVVAIPVEQLYSGAIVEGTYPILNSSRKPCKPGAVLS 180

Query: 181 LSIRYTPVDRTTIFDGGMYASPDYQGVSGTYFPLRKGGKVTLYQDAHVEEHGCLPTDFRL 240
           LSI+YTP DR  IF GGMYASPD+QGV GTYFPLRKGGKVTLYQDAHVEE GCLPT+FRL
Sbjct: 181 LSIQYTPADRAAIFQGGMYASPDHQGVIGTYFPLRKGGKVTLYQDAHVEE-GCLPTEFRL 240

Query: 241 ----EYEHRSCWGDICEAISHARHLIYITGWSVYHEVRLVRDGSKAGECMLGDLLKAKSQ 300
               +YEHRSCW DI E+IS AR LIYITGWSVYH V+LVRDG++  EC+LGDLLKAKSQ
Sbjct: 241 HGGVQYEHRSCWDDISESISQARRLIYITGWSVYHSVKLVRDGTRK-ECILGDLLKAKSQ 300

Query: 301 EGVRVLLLVWDDPTSHRILGHTKV 317
           EGVRVLLL+WDDPTS  +LG+  V
Sbjct: 301 EGVRVLLLIWDDPTSTSMLGYKTV 322

[Close]

BLAST of Spg015858 vs. ExPASy TrEMBL
Match: A0A1S3BLU4 (Phospholipase D OS=Cucumis melo OX=3656 GN=LOC103490965 PE=3 SV=1)

HSP 1 Score: 487.6 bits (1254), Expect = 3.8e-134
Identity = 241/324 (74.38%), Postives = 272/324 (83.95%), Query Frame = 0


Query: 1   MAGHLYETLSFDGSKHGQGHQSFPFSSANESLKVLLLHGYLDIWVKEAKNLPNMDLFRKK 60
           MAG ++ETLSF GS+HGQG+QS PFSS N SLK+LLLHGYLDIWVKEAKNLPN+D+F K 
Sbjct: 1   MAGRIFETLSFGGSQHGQGNQSLPFSSGNTSLKILLLHGYLDIWVKEAKNLPNLDMFHKT 60

Query: 61  LLGKLSKVSSRAFK----KVAERMTSDPYVTISVSGVVIGRTFVIDNNENPVWMQHFYVP 120
           L    SKVS +  K    +   ++TSDPYVT+SVS  VIGRTFVIDN+ENPVWMQHF VP
Sbjct: 61  LGDMFSKVSFKGSKNSDGEKPPKVTSDPYVTVSVSNAVIGRTFVIDNSENPVWMQHFNVP 120

Query: 121 VAHYGAEVLFVVKDHDAVGSETIGTVAIPVEQLCSGSKVEGAFPILDSSRQPCKPGAVLS 180
           VAHYGAEV FVVKDHD VGS+ +G VAIPVEQL SG+ VEG +PIL+SSR+PCKPGAVLS
Sbjct: 121 VAHYGAEVHFVVKDHDVVGSQIMGVVAIPVEQLYSGAIVEGTYPILNSSRKPCKPGAVLS 180

Query: 181 LSIRYTPVDRTTIFDGGMYASPDYQGVSGTYFPLRKGGKVTLYQDAHVEEHGCLPTDFRL 240
           LSI+YTP DR  IF GGMYASPD+QGV GTYFPLRKGGKVTLYQDAHVEE GCLPT+FRL
Sbjct: 181 LSIQYTPADRAAIFQGGMYASPDHQGVIGTYFPLRKGGKVTLYQDAHVEE-GCLPTEFRL 240

Query: 241 ----EYEHRSCWGDICEAISHARHLIYITGWSVYHEVRLVRDGSKAGECMLGDLLKAKSQ 300
               +YEHRSCW DI E+IS AR LIYITGWSVYH V+LVRDG++  EC+LGDLLKAKSQ
Sbjct: 241 HGGVQYEHRSCWDDISESISQARRLIYITGWSVYHSVKLVRDGTRK-ECILGDLLKAKSQ 300

Query: 301 EGVRVLLLVWDDPTSHRILGHTKV 317
           EGVRVLLL+WDDPTS  +LG+  V
Sbjct: 301 EGVRVLLLIWDDPTSTSMLGYKTV 322

[Close]

BLAST of Spg015858 vs. ExPASy TrEMBL
Match: A0A0A0L757 (Phospholipase D OS=Cucumis sativus OX=3659 GN=Csa_3G038190 PE=3 SV=1)

HSP 1 Score: 484.6 bits (1246), Expect = 3.2e-133
Identity = 240/324 (74.07%), Postives = 272/324 (83.95%), Query Frame = 0


Query: 1   MAGHLYETLSFDGSKHGQGHQSFPFSSANESLKVLLLHGYLDIWVKEAKNLPNMDLFRKK 60
           MAG ++ETLSF GS+HGQG+QS PFSS N SLK+LLLHGYLDIWVKEAKNLPN+D+F K 
Sbjct: 1   MAGRIFETLSFGGSQHGQGNQSLPFSSGNTSLKILLLHGYLDIWVKEAKNLPNLDMFHKT 60

Query: 61  LLGKLSKVSSRAFK----KVAERMTSDPYVTISVSGVVIGRTFVIDNNENPVWMQHFYVP 120
           L    SKVS +  K    +  +++TSDPYVTISVS  VIGRTFVIDN+ENPVWMQHF +P
Sbjct: 61  LGDMFSKVSFKGSKNSNGEKPQKVTSDPYVTISVSNAVIGRTFVIDNSENPVWMQHFDIP 120

Query: 121 VAHYGAEVLFVVKDHDAVGSETIGTVAIPVEQLCSGSKVEGAFPILDSSRQPCKPGAVLS 180
           VAHYGAEV FVVKDHD VGS+ +G VAIPVEQL SG+ VEG +PIL+SSR+PCKPGAVLS
Sbjct: 121 VAHYGAEVHFVVKDHDVVGSQIMGVVAIPVEQLYSGAIVEGTYPILNSSRKPCKPGAVLS 180

Query: 181 LSIRYTPVDRTTIFDGGMYASPDYQGVSGTYFPLRKGGKVTLYQDAHVEEHGCLPTDFRL 240
           LSI+YTP DR  IF GGMYASPD+QGV  TYFPLRKGGKVTLYQDAHVEE GCLPTD+RL
Sbjct: 181 LSIQYTPADRAAIFRGGMYASPDHQGVLCTYFPLRKGGKVTLYQDAHVEE-GCLPTDYRL 240

Query: 241 ----EYEHRSCWGDICEAISHARHLIYITGWSVYHEVRLVRDGSKAGECMLGDLLKAKSQ 300
               +YEHRSCW DI E+IS AR LIYITGWSVYH V+LVRDG++  EC+LGDLLKAKSQ
Sbjct: 241 HGGVQYEHRSCWDDITESISQARRLIYITGWSVYHSVKLVRDGTRK-ECILGDLLKAKSQ 300

Query: 301 EGVRVLLLVWDDPTSHRILGHTKV 317
           EGVRVLLL+WDDPTS  +LG+  V
Sbjct: 301 EGVRVLLLIWDDPTSTSMLGYKTV 322

[Close]

BLAST of Spg015858 vs. ExPASy TrEMBL
Match: A0A6J1FIW4 (Phospholipase D OS=Cucurbita moschata OX=3662 GN=LOC111444679 PE=3 SV=1)

HSP 1 Score: 477.2 bits (1227), Expect = 5.2e-131
Identity = 244/324 (75.31%), Postives = 268/324 (82.72%), Query Frame = 0


Query: 1   MAGHLYETLSFDGSKHGQGHQSFPFSSANESLKVLLLHGYLDIWVKEAKNLPNMDLFRKK 60
           MAG ++ETLSF GS+HGQ +QS PFSS N SLK LLLHGYLDI VK AKNLPNMD+F K 
Sbjct: 1   MAGRIFETLSF-GSQHGQSNQSLPFSSGNASLKFLLLHGYLDISVKSAKNLPNMDMFHKT 60

Query: 61  LLGKLSKVSSRAFK----KVAERMTSDPYVTISVSGVVIGRTFVIDNNENPVWMQHFYVP 120
           L   LSKVS ++ K    + A ++TSDPYVTISVSG VIGRTFVIDN+ENPVWMQHF VP
Sbjct: 61  LGDVLSKVSFKSSKNSDGQKAPKVTSDPYVTISVSGAVIGRTFVIDNSENPVWMQHFNVP 120

Query: 121 VAHYGAEVLFVVKDHDAVGSETIGTVAIPVEQLCSGSKVEGAFPILDSSRQPCKPGAVLS 180
           VAHYGAEV FVVKDHD VGS+ IG VAIP+EQL SG  VEG +PIL+SSR+PCKPGAVLS
Sbjct: 121 VAHYGAEVHFVVKDHDVVGSQIIGVVAIPIEQLYSGDVVEGTYPILNSSRKPCKPGAVLS 180

Query: 181 LSIRYTPVDRTTIFDGGMYASPDYQGVSGTYFPLRKGGKVTLYQDAHVEEHGCLPTDFRL 240
           LSIRY P DR  IF GGMYAS D+QGV GTYFPLRKGGKVTLYQDAHVE+ GCLP+DF+L
Sbjct: 181 LSIRYIPADRAAIFHGGMYASSDHQGVPGTYFPLRKGGKVTLYQDAHVED-GCLPSDFKL 240

Query: 241 ----EYEHRSCWGDICEAISHARHLIYITGWSVYHEVRLVRDGSKAGECMLGDLLKAKSQ 300
               EYEHRSCW DI E+IS AR LIYITGWSVYH VRLVRDG KA EC LGDLLKAKSQ
Sbjct: 241 HGGVEYEHRSCWDDITESISQARRLIYITGWSVYHNVRLVRDG-KAKECSLGDLLKAKSQ 300

Query: 301 EGVRVLLLVWDDPTSHRILGHTKV 317
           EGVRVLLL+WDDPTS  +LG+  V
Sbjct: 301 EGVRVLLLIWDDPTSTSMLGYKTV 321

[Close]

BLAST of Spg015858 vs. TAIR 10
Match: AT2G42010.1 (phospholipase D beta 1 )

HSP 1 Score: 375.6 bits (963), Expect = 4.1e-104
Identity = 181/287 (63.07%), Postives = 224/287 (78.05%), Query Frame = 0


Query: 31  SLKVLLLHGYLDIWVKEAKNLPNMDLFRKKLLGKLSKVSSRAFKKVAERMTSDPYVTISV 90
           SLKVLLLHG LDIW+  AKNLPNMD+F K L     ++  +   ++  ++TSDPYV++SV
Sbjct: 265 SLKVLLLHGNLDIWIYHAKNLPNMDMFHKTLGDMFGRLPGKIEGQLTSKITSDPYVSVSV 324

Query: 91  SGVVIGRTFVIDNNENPVWMQHFYVPVAHYGAEVLFVVKDHDAVGSETIGTVAIPVEQLC 150
           +G VIGRT+V+ N+ENPVWMQHFYVPVAH+ AEV FVVKD D VGS+ IG V IPVEQ+ 
Sbjct: 325 AGAVIGRTYVMSNSENPVWMQHFYVPVAHHAAEVHFVVKDSDVVGSQLIGLVTIPVEQIY 384

Query: 151 SGSKVEGAFPILDSSRQPCKPGAVLSLSIRYTPVDRTTIFDGGMYASPDYQGVSGTYFPL 210
           SG+K+EG +PIL+S+ +PCKPGA LSLSI+YTP+D+ +++  G+ A PDYQGV GTYFPL
Sbjct: 385 SGAKIEGTYPILNSNGKPCKPGANLSLSIQYTPMDKLSVYHHGVGAGPDYQGVPGTYFPL 444

Query: 211 RKGGKVTLYQDAHVEEHGCLP---TDFRLEYEHRSCWGDICEAISHARHLIYITGWSVYH 270
           RKGG V LYQDAHV E G LP    D  + YEH  CW D+ +AI  AR LIYITGWSV+H
Sbjct: 445 RKGGTVRLYQDAHVPE-GMLPGIRLDNGMSYEHGKCWHDMFDAIRQARRLIYITGWSVWH 504

Query: 271 EVRLVRDG-SKAGECMLGDLLKAKSQEGVRVLLLVWDDPTSHRILGH 314
           +V+L+RD    A EC LG+LL++KSQEGVRVLLL+WDDPTS  ILG+
Sbjct: 505 KVKLIRDKLGPASECTLGELLRSKSQEGVRVLLLIWDDPTSRSILGY 550

[Close]

BLAST of Spg015858 vs. TAIR 10
Match: AT4G11850.1 (phospholipase D gamma 1 )

HSP 1 Score: 374.8 bits (961), Expect = 7.0e-104
Identity = 188/311 (60.45%), Postives = 235/311 (75.56%), Query Frame = 0


Query: 7   ETLSF-DGSKHGQGHQSFPFSSANESLKVLLLHGYLDIWVKEAKNLPNMDLFRKKLLGKL 66
           ET+S   GS HG G Q  PF++++ SL+V LLHG LDIWVKEAK+LPNMD F  +L G L
Sbjct: 9   ETMSMGGGSSHGGGQQYVPFATSSGSLRVELLHGNLDIWVKEAKHLPNMDGFHNRLGGML 68

Query: 67  SKVSSRAFK-KVAERMTSDPYVTISVSGVVIGRTFVIDNNENPVWMQHFYVPVAHYGAEV 126
           S +  +  + + + ++TSDPYVT+S+SG VIGRTFVI N+ENPVWMQHF VPVAH  AEV
Sbjct: 69  SGLGRKKVEGEKSSKITSDPYVTVSISGAVIGRTFVISNSENPVWMQHFDVPVAHSAAEV 128

Query: 127 LFVVKDHDAVGSETIGTVAIPVEQLCSGSKVEGAFPILDSSRQPCKPGAVLSLSIRYTPV 186
            FVVKD D +GS+ +G V IP EQLCSG+++EG FPIL+SS +PCK GAVL LSI+YTP+
Sbjct: 129 HFVVKDSDIIGSQIMGAVGIPTEQLCSGNRIEGLFPILNSSGKPCKQGAVLGLSIQYTPM 188

Query: 187 DRTTIFDGGMYASPDYQGVSGTYFPLRKGGKVTLYQDAHVEEHGCLPT---DFRLEYEHR 246
           +R  ++  G+ +  +  GV GTYFPLRKGG+VTLYQDAHV++ G LP+   D  ++Y H 
Sbjct: 189 ERMRLYQMGVGSGNECVGVPGTYFPLRKGGRVTLYQDAHVDD-GTLPSVHLDGGIQYRHG 248

Query: 247 SCWGDICEAISHARHLIYITGWSVYHEVRLVRDGSKAGECMLGDLLKAKSQEGVRVLLLV 306
            CW D+ +AI  AR LIYITGWSV+H VRLVR  +   E  LG+LLK KSQEGVRVL+LV
Sbjct: 249 KCWEDMADAIRQARRLIYITGWSVFHPVRLVRRTNDPTEGTLGELLKVKSQEGVRVLVLV 308

Query: 307 WDDPTSHRILG 313
           WDDPTS  +LG
Sbjct: 309 WDDPTSRSLLG 318

[Close]

BLAST of Spg015858 vs. TAIR 10
Match: AT4G00240.1 (phospholipase D beta 2 )

HSP 1 Score: 362.8 bits (930), Expect = 2.7e-100
Identity = 185/304 (60.86%), Postives = 225/304 (74.01%), Query Frame = 0


Query: 14  SKHGQGHQSFPFSSANESLKVLLLHGYLDIWVKEAKNLPNMDLFRKKLLGKLSKVSSRAF 73
           S H  G    PF  A  SLKVLLLHG LDIWV  A NLPN+DLF K L      +++   
Sbjct: 94  SPHSPGMHIVPFGKA--SLKVLLLHGNLDIWVSCANNLPNLDLFHKTLGVVFGGMTNMIE 153

Query: 74  KKVAERMTSDPYVTISVSGVVIGRTFVIDNNENPVWMQHFYVPVAHYGAEVLFVVKDHDA 133
            ++++++TSDPYV+ISV+G VIGRT+VI N+ENPVW QHFYVPVAH+ AEV FVVKD DA
Sbjct: 154 GQLSKKITSDPYVSISVAGAVIGRTYVISNSENPVWQQHFYVPVAHHAAEVHFVVKDSDA 213

Query: 134 VGSETIGTVAIPVEQLCSGSKVEGAFPILDSSRQPCKPGAVLSLSIRYTPVDRTTIFDGG 193
           VGS+ IG V IPVEQ+ SG+++EG + I DS+ +PCKPGA LSLSI+YT +++ +++  G
Sbjct: 214 VGSQLIGIVTIPVEQIYSGARIEGTYSIRDSNGKPCKPGATLSLSIQYTSMNKLSVYHSG 273

Query: 194 MYASPDYQGVSGTYFPLRKGGKVTLYQDAHVEEHGCLP---TDFRLEYEHRSCWGDICEA 253
           + A P YQGV GTYFPLR+GG VTLYQDAHV E G LP       + YEH  CW D+  A
Sbjct: 274 VGAGPYYQGVPGTYFPLREGGSVTLYQDAHVPE-GMLPGIKLGNGMCYEHGKCWHDMFHA 333

Query: 254 ISHARHLIYITGWSVYHEVRLVRD-GSKAGECMLGDLLKAKSQEGVRVLLLVWDDPTSHR 313
           I  AR LIYITGWSV+H VRLVRD    + EC LG+LL++KSQEGVRVLLLVWDDPTS  
Sbjct: 334 ICQARRLIYITGWSVWHNVRLVRDKEDPSSECRLGELLRSKSQEGVRVLLLVWDDPTSQN 393

[Close]

BLAST of Spg015858 vs. TAIR 10
Match: AT4G11840.1 (phospholipase D gamma 3 )

HSP 1 Score: 356.7 bits (914), Expect = 2.0e-98
Identity = 185/318 (58.18%), Postives = 230/318 (72.33%), Query Frame = 0


Query: 7   ETLSFDG---SKHGQ--GHQSFPFSSANESLKVLLLHGYLDIWVKEAKNLPNMDLFRKKL 66
           ET+S  G   ++ GQ    Q  PF +++ SL+V LLHG LDIWVKEAK+LPNMD F   L
Sbjct: 9   ETMSMGGGSSNEFGQWLDKQLVPFDTSSGSLRVELLHGNLDIWVKEAKHLPNMDGFHNTL 68

Query: 67  LGKLSKVSSRAFKKV----AERMTSDPYVTISVSGVVIGRTFVIDNNENPVWMQHFYVPV 126
           +G +     R   KV    + ++TSDPYVT+S+SG VIGRTFVI N+ENPVWMQHF VPV
Sbjct: 69  VGGMFFGLGRRNHKVDGENSSKITSDPYVTVSISGAVIGRTFVISNSENPVWMQHFDVPV 128

Query: 127 AHYGAEVLFVVKDHDAVGSETIGTVAIPVEQLCSGSKVEGAFPILDSSRQPCKPGAVLSL 186
           AH  A+V FVVKD D +GS+ IG V IP EQLCSG+++EG FPIL+S  +PCK GAVLSL
Sbjct: 129 AHSAAKVHFVVKDSDIIGSQIIGAVEIPTEQLCSGNRIEGLFPILNSRGKPCKQGAVLSL 188

Query: 187 SIRYTPVDRTTIFDGGMYASPDYQGVSGTYFPLRKGGKVTLYQDAHVEEHGCLPT---DF 246
           SI+Y P++R  ++  G+    +  GV GTYFPLRKGG+VTLYQDAHV++ G LP+   D 
Sbjct: 189 SIQYIPMERMRLYQKGVGFGVECVGVPGTYFPLRKGGRVTLYQDAHVDD-GTLPSVHLDG 248

Query: 247 RLEYEHRSCWGDICEAISHARHLIYITGWSVYHEVRLVRDGSKAGECMLGDLLKAKSQEG 306
            ++Y H  CW D+ +AI  AR LIYITGWSV+H VRLVR  +   +  LG+LLK KSQEG
Sbjct: 249 GIQYRHGKCWEDMADAIRRARRLIYITGWSVFHPVRLVRRNNDPTQGTLGELLKVKSQEG 308

Query: 307 VRVLLLVWDDPTSHRILG 313
           VRVL+LVWDDPTS  +LG
Sbjct: 309 VRVLVLVWDDPTSRSLLG 325

[Close]

BLAST of Spg015858 vs. TAIR 10
Match: AT4G11830.2 (phospholipase D gamma 2 )

HSP 1 Score: 355.5 bits (911), Expect = 4.4e-98
Identity = 182/307 (59.28%), Postives = 227/307 (73.94%), Query Frame = 0


Query: 13  GSKHGQG----HQSFPFSSANESLKVLLLHGYLDIWVKEAKNLPNMDLFRKKLLGKLS-K 72
           GS H  G     Q  P ++++ SL V LLHG LDIWVKEAK+LPNM  +R KL+G +S  
Sbjct: 6   GSNHEFGQWLDQQLVPLATSSGSLMVELLHGNLDIWVKEAKHLPNMICYRNKLVGGISFS 65

Query: 73  VSSRAFKKV----AERMTSDPYVTISVSGVVIGRTFVIDNNENPVWMQHFYVPVAHYGAE 132
              R  +KV    + + TSDPYVT+S+SG VIGRTFVI N+ENPVWMQHF VPVAH  AE
Sbjct: 66  ELGRRIRKVDGEKSSKFTSDPYVTVSISGAVIGRTFVISNSENPVWMQHFDVPVAHSAAE 125

Query: 133 VLFVVKDHDAVGSETIGTVAIPVEQLCSGSKVEGAFPILDSSRQPCKPGAVLSLSIRYTP 192
           V FVVKD+D +GS+ IG V IP +QLCSG+++EG FPIL+SS +PC+ GA+LSLSI+YTP
Sbjct: 126 VHFVVKDNDPIGSKIIGVVGIPTKQLCSGNRIEGLFPILNSSGKPCRKGAMLSLSIQYTP 185

Query: 193 VDRTTIFDGGMYASPDYQGVSGTYFPLRKGGKVTLYQDAHVEEHGCLPT---DFRLEYEH 252
           ++R  ++  G+ +  +  GV GTYFPLRKGG+VTLYQDAHV++ G LP+   D  ++Y H
Sbjct: 186 MERMRLYQKGVGSGVECVGVPGTYFPLRKGGRVTLYQDAHVDD-GTLPSVHLDGGIQYRH 245

Query: 253 RSCWGDICEAISHARHLIYITGWSVYHEVRLVRDGSKAGECMLGDLLKAKSQEGVRVLLL 308
             CW D+ +AI  AR LIYITGWSV+H VRLVR  +   E  LG+LLK KSQEGVRVL+L
Sbjct: 246 GKCWEDMADAIRRARRLIYITGWSVFHPVRLVRRNNDPTEGTLGELLKVKSQEGVRVLVL 305

The following BLAST results are available for this feature:

BLAST of Spg015858 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022152769.1	2.5e-135	75.62	phospholipase D beta 1-like [Momordica charantia]	[more]
XP_008448951.1	7.9e-134	74.38	PREDICTED: phospholipase D beta 1-like [Cucumis melo] >TYK19444.1 phospholipase ...	[more]
XP_011650436.1	6.7e-133	74.07	phospholipase D beta 1 [Cucumis sativus] >KGN55946.1 hypothetical protein Csa_01...	[more]
XP_038904877.1	4.3e-132	74.77	phospholipase D beta 1-like [Benincasa hispida]	[more]
KAG6596811.1	1.1e-130	75.31	Phospholipase D beta 1, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of Spg015858 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P93733	5.7e-103	63.07	Phospholipase D beta 1 OS=Arabidopsis thaliana OX=3702 GN=PLDBETA1 PE=1 SV=4	[more]
Q9T053	9.8e-103	60.45	Phospholipase D gamma 1 OS=Arabidopsis thaliana OX=3702 GN=PLDGAMMA1 PE=1 SV=1	[more]
O23078	3.9e-99	60.86	Phospholipase D beta 2 OS=Arabidopsis thaliana OX=3702 GN=PLDBETA2 PE=1 SV=3	[more]
Q9T052	2.8e-97	58.18	Phospholipase D gamma 3 OS=Arabidopsis thaliana OX=3702 GN=PLDGAMMA3 PE=1 SV=1	[more]
Q9T051	6.2e-97	59.28	Phospholipase D gamma 2 OS=Arabidopsis thaliana OX=3702 GN=PLDGAMMA2 PE=1 SV=3	[more]

BLAST of Spg015858 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DH01	1.2e-135	75.62	Phospholipase D OS=Momordica charantia OX=3673 GN=LOC111020410 PE=3 SV=1	[more]
A0A5D3D7C2	3.8e-134	74.38	Phospholipase D OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold443G0096...	[more]
A0A1S3BLU4	3.8e-134	74.38	Phospholipase D OS=Cucumis melo OX=3656 GN=LOC103490965 PE=3 SV=1	[more]
A0A0A0L757	3.2e-133	74.07	Phospholipase D OS=Cucumis sativus OX=3659 GN=Csa_3G038190 PE=3 SV=1	[more]
A0A6J1FIW4	5.2e-131	75.31	Phospholipase D OS=Cucurbita moschata OX=3662 GN=LOC111444679 PE=3 SV=1	[more]

BLAST of Spg015858 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G42010.1	4.1e-104	63.07	phospholipase D beta 1	[more]
AT4G11850.1	7.0e-104	60.45	phospholipase D gamma 1	[more]
AT4G00240.1	2.7e-100	60.86	phospholipase D beta 2	[more]
AT4G11840.1	2.0e-98	58.18	phospholipase D gamma 3	[more]
AT4G11830.2	4.4e-98	59.28	phospholipase D gamma 2	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000008	C2 domain	SMART	SM00239	C2_3c	coord: 40..158 e-value: 2.3E-17 score: 73.7
IPR000008	C2 domain	PFAM	PF00168	C2	coord: 39..161 e-value: 5.4E-27 score: 94.1
IPR000008	C2 domain	PROSITE	PS50004	C2	coord: 20..159 score: 15.63434
IPR035892	C2 domain superfamily	GENE3D	2.60.40.150	C2 domain	coord: 34..170 e-value: 1.7E-22 score: 81.9
IPR035892	C2 domain superfamily	SUPERFAMILY	49562	C2 domain (Calcium/lipid-binding domain, CaLB)	coord: 37..184
IPR015679	Phospholipase D family	PANTHER	PTHR18896	PHOSPHOLIPASE D	coord: 7..314
None	No IPR available	PANTHER	PTHR18896:SF130	PHOSPHOLIPASE D BETA 1-LIKE	coord: 7..314
None	No IPR available	CDD	cd04015	C2_plant_PLD	coord: 33..182 e-value: 8.89339E-61 score: 189.438
None	No IPR available	SUPERFAMILY	56024	Phospholipase D/nuclease	coord: 201..308

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg015858.1	Spg015858.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0046470	phosphatidylcholine metabolic process
biological_process	GO:0009395	phospholipid catabolic process
cellular_component	GO:0005886	plasma membrane
molecular_function	GO:0005509	calcium ion binding
molecular_function	GO:0070290	N-acylphosphatidylethanolamine-specific phospholipase D activity
molecular_function	GO:0004630	phospholipase D activity