Spg015821 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg015821
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Description(-)-germacrene D synthase-like
Locationscaffold6: 44432971 .. 44438731 (+)
RNA-Seq ExpressionSpg015821
SyntenySpg015821
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAGTTGTTCTTTTTTTCCTATTTTTCATCGCATTCCAATTAATGGACTCTCAATTTCCATCATCCATCAATGGTGAAACTCGTCGTTCAACAAACTTCCCACCAAATACATGGGGAGATTATTTTCTTTCCAACACTTTTGAGACCATGGTAGTGATTTTTTTTTTTTAACTAATTTCTTCTTATTAATAATATTATGTACAAACAATTTTGTTCAATGTTTGTTCTTTGAATTATTTTTTTAAATTTTTTTTTTTTTGATTTAGGAGTGCATATAGTACGATGAAACTAACTATATAATGTTCTCTTTTTTTTTTTTTTTTTTTGGAAATCGTTCTTTCTTTCTTTCTTTTTTCCACTCACAAATTTAGGATTAAGCCTTTAATCTTACAAAAAGTATTGGCTGTGTTACTCAAGTTAACAAATTGGCTAAAAAAAATTTTTTAGGAAAAAATTGGCTATAATTATAGTTCTTTGCAAAATTGGCTATTTTCCTTTTTTTTTTTTTTTTTTCTAGTTATCTAAAAGGGTTATGGTAATGGGTAGCAATTTAAGACTTGTTATTTGCAAAGATAACAACTTTAAAAAAAAAATATGGCAAATCTATCAATGATAATAATAGCCTTGTTAGTTGCTATATTTACAAATATGGCAAATCTATCAATGATAGACATCTATTACTGGTAGACTTCAATTACAATGCAATTAATCTATGAGTGATAGACTACTATCACTAATAGAATAGTCTATCAACAATAATTGAAATTGTGATTGAAGTCTATCGTTGTTAGAATTCTATCCCTAAGGGCCCGTTTGGTACAAGGTTTTTGGAGATGGGAATGGGCATGGGTTTCTAATACCCTTTGTTTGTTTCAAGATTTCTGTGGTGGGCCCAGGAAATAACGCATTCCCAGGCATCATTTATACCCATAAGCAAGGGTTTTCTATGCCCTAGCAAAAGCATGGGTTTTCTCTTTTCCCATCTCTTCTTTTTTATTTTTATATGCAATTTTATTTTGAATATAGTTAATTAATTAACTAATGGATACTAAATTAAAAATAATATAATAAATTATTTAATTAGATTAAATAATAATTTGTCAAGAATTTCATTTTTTTTAAACAAATACTTTTTTCTAGGACTTTTAAACAAATACTTAGTCCAAGTTTATTAAAGGGATGTAACTACATATTTACTAAAATTATAATTAACTAGTCAATATTATCGAAAATATATTTAATTATTGAATTAAAATCATTTAAATGAATTTAGTATAAAAATTCATTTTTCAATAATTATAATTAATTACTTATCGATTATTTATAATCAATTAATTACTTTTTTAAATTTCCTTATAATAAATAAATAATCATATTATTATTAAATATTTATCTAATTAATTTTTATTGATACAATATATTAAATTATTGTAGTTAAAGTCATTTATTAAAAAATTAATTAAATAATTTTAGTTTGTTAAATTAACATAGTTTATCCCATTAAAATCATTTCATGATTATTATTTCATTAATTTTTATTAATAGTTTGAATTAATTTTGATTATTTTATTGATTAATTTATTAAAATTACATAAAGTTTACCTGATTAATTCTAAAAAATAAATACAGACATAATTTGTTCTCATAAAATCTTGATGGCATTCCCATGCACAACCAAACACAGTTATCTTTGATTCCCAGCAATCTTATTCCCAGGCATCATGTATTCTCAGGCATCTATAAAACCTTAAACCAAACGGGCCCTAATAGGCTTTCGTTTTGTTATATTTGTTAATATTTTTGGCAAATCTAATAGTGATAGACATCTACCACTGATAGACTTCAATCACAATGCATGTAGTCTATCAATGGTAGATTACTATTATTGGTAGAATAGTCTATCAACAATAATTACACTTGTGATTGAAATTTATCGTTGACAGATTTATATCACTGATAGACTTCAATTTTGCTATATTTGTAAATAGTTTTAGATTTTTCTATATTTACAATTATTTTAGTTGATTTTGCTATTTGTGCAAACAATAATCCTATCTAAAAGCTTTCCAAATATAATTGGATGGAGTCCAAAACTTCTTTCAACCACAATAATGAGATGGAAAAACTTCATTTCTACCTCATAATTGGTCGAGTGAGTCAAATTTAACATTAAGCTTGGAATCATCAAATTAATTTTAAAGATATTTGATCCTACTTGGTTTGAATTTGGGCAATTTTGATTTTGAGTGTTTTATTCATTATATTTTATGCAATTCATTCGCTTTATTGGTTAATCGGCGACTCTCTCTAATTCAATTTTAAAGAGGTATTATTTACCTCTAGTGAAGTTTACTTTAATAAAAATGCAAATTGAAAGTTGAAATTAAATTACTTAGAAGTAGAGGTAAAATGAAATTTTTATTAACCAAAATTTGTACTTCCTTCTTCTTTTTAAGGAAATAATGGAACATAAGATTGAGGAAGAAGTGGAAGAACTCAAGGAGGAAGTGAGAAAATTGTTGATTACTTGTGTTGAGAAGCCTTCCCAACAGCTAAGTTTGATTGATTCAATCCAACGATTGGGAGTCGCTTATCATTTCGATACCGAGATCAATGAAGCACTTGATCATGTTTATAACAATTACAATGTTATTACCAAAGAAAGTGATGAACAAATTCATGATGTTGCTCTAAGGTTTCGCTTGCTTAGGCAACAAAGATACTCTATTTCATGTGGTAGGTTGTCACTTATTTATTCTCTATTTTCTTTTTTTTTTTTTGTTTTAATCATAAATTTAGTCCTTGTATGTACTTTGATCGTTATGTAGTTTAGTCTCTGAATTTTAAAAAGTCTTAATAATGTCTCTAAACTTTAAATTATGGTTTTTATATTCCATCCCTATTATTTTTCAAGTATGTCGAGGTAATTTAATTGATGGTTAAATATATGACCTTAAATAACCTAAAGTTTGATACTTTTTTTTTTTCTTTCGAGTTCAACAACATTTGGGGTGGGAATTCGAATCTCCGACCTCTTGGTAGAGGATACAAGTCAATTACCGATGAACTAGGTTCATTTTGGTATTTTTGATGCTTGGGTTAAGTATGTGGGAATTTGGAATTTTTTTGGAGTTGTGCTTTAAAGTGAAATACTTTTTTATCTATAATTACTTTGGAGAAAAACACTAATTTGTGCTCATTCTAAAAATATTTTTCATATCTATTGTGAGGATCGAGAATGGGAGGGGTACATAAAATGAACCAAAGACCACATTTGAATAAGAGGGATCTTGAGGACATGAAAGTATGGTTAAGATAAGCTTAAAGAATTGATAGATACAACCTATACCAACAAGGTGCACTTTCCTTTTTGGTGACTCAATCATAGGAACTTTGAAGTTAAGCGTGCTTGGCTTGGAGAAGTTTTATGTTGGGTGATCTCCTGAGAAAGTTCACCTCTTGAGAACTTTCTTAGGAAACATGTGAGTGATGACAAAACATCCTAAAAGAACCCCATATTGGTTTGTGGGGACATTCTTCACTCTTAGAAGTAGTTTCAGACAAGTATGGTGGCGTGACCACAGATCGCAGGGGATACAGGGGAATGTCGGAGTCGTCAGATTTCGAATCCTGGACCTGGGACATTACATCTATTTTAAATAACGCTTTTAGAATTTATGAAATCACTACACATCATGAAAAACTAAAAAAGGTGTTTCTTGATTGACTAAAAGCTAAACATAGCTAAGTAGTAATTGTTATACCTTAGCTCAGAAGTTCAAATCTCTAAAAACATCAACAAATTCAGACTAAAAATATGATTAATTCTTGAGTCACTAAGGATTAGGTTTCAATTATATATTGAATTGATCTAACTTGCCAACTACTTATATATTCCGAGTTATATATGATTGTTAGTTGTCAAAGAATTCAGCTTAAATGTCAAAATAATTTATTGAAATATTTGATCTCACTTGCCAACTACAGCTTCAATGTTATATATCTTTATTTTCCATTATTTGGTATATTGAATGAAATTTGTTTAGGCCATGTTTGGTAACTATTTGGTTTTTGGTTTTTGAAAATTAAGCTTTGTTTTCTCCCAATTTCGTTACAATTGTTTTCATCTTTGTTAAGGACACACATTCGAATTCCTAATCAAATTCTAAAAACAAAAACAAGTTTTTGAAAAACTAGGGCCCGTTTGATAACCATTTCAGTTTTTGGTTTTTTGTTTTTCTGTTTTTGGTTTTTGGTTTTTGGTTTTTGGTTTCCTGTTTTTTAAAAACAAAAACCACATATGTTTGATAACTGTTTTTTGTTTTCTGTTTTTAAAAAACAGAAAACCAACCTTGTTTGATAACTGTTTTTGGTTTTCAGTTTTTTAAAAATTAGAAAGATGAAAAGATATTTTAGAGAGAGAAAAATGTGTAAAGCCGTTGAAACAAAAATTTAGAGACACAAATATAAATGTTTTCAAGAGAATTTTTTTTTTTTTTTTTGAAAAGACGAGAGAAAAGTTAAACATAGAAAATTTAGAGAGATGAAAGAAAATGTTTAGAGAAAGAATATTAGTTAGACTCGAGAAAAGATATTAGTTAGAGAAAAAATAGAAAACAAGTTAGAGTAAAAGCGATAAATTTTAGATTAAAAAAGTTAAATAAAAAAAGAAAATTTAGAGAGATAGTAAAGTTAAGAGTAAAAGATATGTTAAAGAACTAATGATAAATTAGAAAAATAAAGAAAAGTTGTAGCGATAGAAAATTTTGGTGACTTTTTTTTTCCAAAAGAAAAATTAGAACATGTTTATTGAGGAGAGAAAAGGTATTGACAAATTTTTAGAAATAAACAAGTTATAGAGAAAAAATAAAGTTTTGAGAGAGAAAATTTTAGAGAGAGAAAATTTTAGAGAGAGAAAAAAAATAGAGAAAGAAATTTGTTGGAGAGAGAGGTAAAAGAGGAGAAAAGTAAGCATTAGAAGAAAAAATGAGTTTTTGAAAAACAGAAATCTGTTTTTAAAAACTACTTTTTTTGGTTTTTGAAATTTACTTATAATTTCAAAAACGTTTTTAAAAAACAGAAAACAGAAAACAGAAATGGTTATCAAACACCCCTGTTTTTAAAAAATGAAAAACAAAAACAGAAAACAGAAAACGGAATGGTTATCAAACGGGCCCTACTTTTTTTAGTTTCCAAAACTTGACTTAATTTTTGAAAACATAGGACGAAGAAGGTAGATAACAAAACCAAGAAACTTATATATGGAAGTAGTGTTTTTAAGCTTAATTTTCTATTCATCTCATTGACGAACAAAACTTGAAAAACAGATATCTTCGACAAGTTTACCGACAACGAAGGAAATTTCAAGGAATCACTTGCCAAAGATGTCCGAGGAATATTAAGCTTATACGAAGCATCTCATATACGGGTCCATGGAGAAGACGTGCTCGAACGAGCCCTTGCTTTCACGACTACAAACTTAAAAATAGCCATAAGTGCAAGCCATGAAGGCAGCTGTGGTCTTGCAGCACAAGTGAGCTATGCATTGAAATGGCCACTCTTTAAAGCCATGCCTAGGCTTGCAGCAATACATTACATATCTTTCTATGAAGAAGATCCATTGCACCATCCTGCCTTGCTTTCCTTCGCCAAGCTTGATTACAATTGCTTGCAAAAGCTTTACCAAAAAGAAATTCATGAAATCTCGAGGTAAAAAGAAAAATCTTTAGAGTCCGTAATTGTTAGGTTAAATTATAAGTTTG

mRNA sequence

ATGGACTCTCAATTTCCATCATCCATCAATGGTGAAACTCGTCGTTCAACAAACTTCCCACCAAATACATGGGGAGATTATTTTCTTTCCAACACTTTTGAGACCATGGAAATAATGGAACATAAGATTGAGGAAGAAGTGGAAGAACTCAAGGAGGAAGTGAGAAAATTGTTGATTACTTGTGTTGAGAAGCCTTCCCAACAGCTAAGTTTGATTGATTCAATCCAACGATTGGGAGTCGCTTATCATTTCGATACCGAGATCAATGAAGCACTTGATCATGTTTATAACAATTACAATGTTATTACCAAAGAAAGTGATGAACAAATTCATGATGTTGCTCTAAGGTTTCGCTTGCTTAGGCAACAAAGATACTCTATTTCATGTGATATCTTCGACAAGTTTACCGACAACGAAGGAAATTTCAAGGAATCACTTGCCAAAGATGTCCGAGGAATATTAAGCTTATACGAAGCATCTCATATACGGGTCCATGGAGAAGACGTGCTCGAACGAGCCCTTGCTTTCACGACTACAAACTTAAAAATAGCCATAAGTGCAAGCCATGAAGGCAGCTGTGGTCTTGCAGCACAAGTGAGCTATGCATTGAAATGGCCACTCTTTAAAGCCATGCCTAGGCTTGCAGCAATACATTACATATCTTTCTATGAAGAAGATCCATTGCACCATCCTGCCTTGCTTTCCTTCGCCAAGCTTGATTACAATTGCTTGCAAAAGCTTTACCAAAAAGAAATTCATGAAATCTCGAGGTAA

Coding sequence (CDS)

ATGGACTCTCAATTTCCATCATCCATCAATGGTGAAACTCGTCGTTCAACAAACTTCCCACCAAATACATGGGGAGATTATTTTCTTTCCAACACTTTTGAGACCATGGAAATAATGGAACATAAGATTGAGGAAGAAGTGGAAGAACTCAAGGAGGAAGTGAGAAAATTGTTGATTACTTGTGTTGAGAAGCCTTCCCAACAGCTAAGTTTGATTGATTCAATCCAACGATTGGGAGTCGCTTATCATTTCGATACCGAGATCAATGAAGCACTTGATCATGTTTATAACAATTACAATGTTATTACCAAAGAAAGTGATGAACAAATTCATGATGTTGCTCTAAGGTTTCGCTTGCTTAGGCAACAAAGATACTCTATTTCATGTGATATCTTCGACAAGTTTACCGACAACGAAGGAAATTTCAAGGAATCACTTGCCAAAGATGTCCGAGGAATATTAAGCTTATACGAAGCATCTCATATACGGGTCCATGGAGAAGACGTGCTCGAACGAGCCCTTGCTTTCACGACTACAAACTTAAAAATAGCCATAAGTGCAAGCCATGAAGGCAGCTGTGGTCTTGCAGCACAAGTGAGCTATGCATTGAAATGGCCACTCTTTAAAGCCATGCCTAGGCTTGCAGCAATACATTACATATCTTTCTATGAAGAAGATCCATTGCACCATCCTGCCTTGCTTTCCTTCGCCAAGCTTGATTACAATTGCTTGCAAAAGCTTTACCAAAAAGAAATTCATGAAATCTCGAGGTAA

Protein sequence

MDSQFPSSINGETRRSTNFPPNTWGDYFLSNTFETMEIMEHKIEEEVEELKEEVRKLLITCVEKPSQQLSLIDSIQRLGVAYHFDTEINEALDHVYNNYNVITKESDEQIHDVALRFRLLRQQRYSISCDIFDKFTDNEGNFKESLAKDVRGILSLYEASHIRVHGEDVLERALAFTTTNLKIAISASHEGSCGLAAQVSYALKWPLFKAMPRLAAIHYISFYEEDPLHHPALLSFAKLDYNCLQKLYQKEIHEISR
Homology
BLAST of Spg015821 vs. NCBI nr
Match: XP_022154020.1 ((+)-gamma-cadinene synthase-like [Momordica charantia])

HSP 1 Score: 409.5 bits (1051), Expect = 2.2e-110
Identity = 204/255 (80.00%), Postives = 227/255 (89.02%), Query Frame = 0

Query: 4   QFPSSINGETRRSTNFPPNTWGDYFLSNTFETMEIMEHKIEEEVEELKEEVRKLLITCVE 63
           Q  SSING+TRRS NFPPN WGDYFL NTF +ME ME  IEE+VE +KEEVRKLLITCVE
Sbjct: 13  QSASSINGDTRRSANFPPNIWGDYFLFNTFHSME-MEDNIEEKVEAVKEEVRKLLITCVE 72

Query: 64  KPSQQLSLIDSIQRLGVAYHFDTEINEALDHVYNNYNVITKESDEQIHDVALRFRLLRQQ 123
           KPSQQLSLIDSIQRLG+AYHFDTEINEALDHVY NY+V+  E++E +  VA++FRLLRQQ
Sbjct: 73  KPSQQLSLIDSIQRLGIAYHFDTEINEALDHVYKNYSVVCNENNEDLQIVAMKFRLLRQQ 132

Query: 124 RYSISCDIFDKFTDNEGNFKESLAKDVRGILSLYEASHIRVHGEDVLERALAFTTTNLKI 183
           RYSISCDIFDKFTD+EGNFKESLAKDVRG+LSLYEASH+R+HGEDVLERAL+F+TTNLK 
Sbjct: 133 RYSISCDIFDKFTDDEGNFKESLAKDVRGMLSLYEASHVRIHGEDVLERALSFSTTNLKA 192

Query: 184 AIS-ASHEGSCGLAAQVSYALKWPLFKAMPRLAAIHYISFYEEDPLHHPALLSFAKLDYN 243
           AIS  SHEGSCG+  QV +ALKWP+FKAMPRLAA  YISFYE+DPLHHP LL  AKLDYN
Sbjct: 193 AISNGSHEGSCGIGGQVRHALKWPVFKAMPRLAARSYISFYEQDPLHHPTLLYLAKLDYN 252

Query: 244 CLQKLYQKEIHEISR 258
            LQKLYQKE+HEISR
Sbjct: 253 ILQKLYQKELHEISR 266

BLAST of Spg015821 vs. NCBI nr
Match: XP_022154019.1 ((-)-germacrene D synthase-like [Momordica charantia])

HSP 1 Score: 403.7 bits (1036), Expect = 1.2e-108
Identity = 202/255 (79.22%), Postives = 228/255 (89.41%), Query Frame = 0

Query: 4   QFPSSINGETRRSTNFPPNTWGDYFLSNTFETMEIMEHKIEEEVEELKEEVRKLLITCVE 63
           Q  SS+NG+TRRS NFPPN WGDYFLSN F +M+ ME  IEE+VE LKE VRKLLITCVE
Sbjct: 13  QSASSVNGDTRRSANFPPNIWGDYFLSNIFHSMD-MEDNIEEKVEALKEGVRKLLITCVE 72

Query: 64  KPSQQLSLIDSIQRLGVAYHFDTEINEALDHVYNNYNVITKESDEQIHDVALRFRLLRQQ 123
           KPSQQLSLIDSIQRLG+AYHFDTEINEAL+HVY NY V++ +S+E +  VAL+FRLLRQQ
Sbjct: 73  KPSQQLSLIDSIQRLGIAYHFDTEINEALNHVYKNYTVVSNKSNEDLQIVALKFRLLRQQ 132

Query: 124 RYSISCDIFDKFTDNEGNFKESLAKDVRGILSLYEASHIRVHGEDVLERALAFTTTNLKI 183
           RYSISC+IFDKFTD+EGNFKESLAKDVRG+LSLYEASH+R+HGEDVLERALAF+TTNLK 
Sbjct: 133 RYSISCEIFDKFTDDEGNFKESLAKDVRGMLSLYEASHVRMHGEDVLERALAFSTTNLKA 192

Query: 184 AIS-ASHEGSCGLAAQVSYALKWPLFKAMPRLAAIHYISFYEEDPLHHPALLSFAKLDYN 243
           AIS  SHEGS G  AQV +ALKWP+FKAMPRLAA +YISFYE+DPLHHP LLSFAKLDYN
Sbjct: 193 AISNGSHEGSWGFGAQVRHALKWPVFKAMPRLAARNYISFYEQDPLHHPTLLSFAKLDYN 252

Query: 244 CLQKLYQKEIHEISR 258
            LQKLYQ+E+HEI+R
Sbjct: 253 TLQKLYQRELHEITR 266

BLAST of Spg015821 vs. NCBI nr
Match: XP_022154012.1 ((-)-germacrene D synthase-like [Momordica charantia])

HSP 1 Score: 383.6 bits (984), Expect = 1.3e-102
Identity = 193/252 (76.59%), Postives = 219/252 (86.90%), Query Frame = 0

Query: 7   SSINGETRRSTNFPPNTWGDYFLSNTFETMEIMEHKIEEEVEELKEEVRKLLITCVEKPS 66
           S ING+TRRS NFPPN WGDYFLS TF + EI E  IEE+VE L+EEVRKLL + VEK S
Sbjct: 16  SPINGDTRRSANFPPNIWGDYFLSGTFRSTEI-EDNIEEKVEALEEEVRKLLFSYVEKSS 75

Query: 67  QQLSLIDSIQRLGVAYHFDTEINEALDHVYNNYNVITKESDEQIHDVALRFRLLRQQRYS 126
           QQLSLIDSIQRLG+AYHFDTEINEALDHVY NY+ ++ ES + +H VAL+F LLRQQRYS
Sbjct: 76  QQLSLIDSIQRLGIAYHFDTEINEALDHVYKNYSFVSNESQKDLHIVALKFHLLRQQRYS 135

Query: 127 ISCDIFDKFTDNEGNFKESLAKDVRGILSLYEASHIRVHGEDVLERALAFTTTNLKIAI- 186
           ISC+IFDKFT++EGNFKESLA +VRG+LSLYEASH+R+HGEDVLERAL F+T NL+ AI 
Sbjct: 136 ISCEIFDKFTNDEGNFKESLANEVRGMLSLYEASHMRMHGEDVLERALTFSTINLEAAIN 195

Query: 187 SASHEGSCGLAAQVSYALKWPLFKAMPRLAAIHYISFYEEDPLHHPALLSFAKLDYNCLQ 246
           + SHEGSCG  AQV +ALKWP+FKAMPRLAA  YISFYEEDPLHHPALLS AKLD+N LQ
Sbjct: 196 NGSHEGSCGFGAQVRHALKWPVFKAMPRLAARSYISFYEEDPLHHPALLSMAKLDFNILQ 255

Query: 247 KLYQKEIHEISR 258
           KLYQKE+HEISR
Sbjct: 256 KLYQKELHEISR 266

BLAST of Spg015821 vs. NCBI nr
Match: XP_008448865.1 (PREDICTED: (-)-germacrene D synthase-like [Cucumis melo])

HSP 1 Score: 371.3 bits (952), Expect = 6.7e-99
Identity = 187/254 (73.62%), Postives = 221/254 (87.01%), Query Frame = 0

Query: 8   SINGETRRSTNFPPNTWGDYFLSNTFETMEIMEHKIEEEVEELKEEVRKLLITCVEKPSQ 67
           SING+ RRS NFPPN WGDYFLSNTF+TME ME+++E++VEE+KEEVRK+LITCVEKP +
Sbjct: 19  SINGDARRSANFPPNIWGDYFLSNTFQTME-MENEVEKKVEEMKEEVRKMLITCVEKPFE 78

Query: 68  QLSLIDSIQRLGVAYHFDTEINEALDHVYNNY-NVITKESDEQIHDVALRFRLLRQQRYS 127
           QL LIDSIQ+LG+AYHF+ EI EALD VY NY N+IT ES+E IH V+LRFRLLRQQRYS
Sbjct: 79  QLILIDSIQQLGLAYHFEIEIKEALDDVYMNYNNLITSESNEDIHIVSLRFRLLRQQRYS 138

Query: 128 ISCDIFDKFTDNEGNFKESLAKDVRGILSLYEASHIRVHGEDVLERALAFTTTNLKIAI- 187
           ISCDIF+KFTD+EGNFKE+L KDV+G+LSLY+ASH+R+HGE++LERA AFTTTNL+ AI 
Sbjct: 139 ISCDIFNKFTDDEGNFKETLTKDVKGMLSLYQASHMRMHGEEILERAFAFTTTNLRAAIN 198

Query: 188 --SASHEGSCGLAAQVSYALKWPLFKAMPRLAAIHYISFYEEDPLHHPALLSFAKLDYNC 247
             + SHE       ++SYALKWP FKAMPRLA+ +YIS YEEDPLH+P LL FAKLDYNC
Sbjct: 199 NSNGSHED-----REISYALKWPFFKAMPRLASRNYISSYEEDPLHNPTLLCFAKLDYNC 258

Query: 248 LQKLYQKEIHEISR 258
           LQKLYQKE+HEISR
Sbjct: 259 LQKLYQKELHEISR 266

BLAST of Spg015821 vs. NCBI nr
Match: XP_004147250.2 ((-)-germacrene D synthase [Cucumis sativus] >KGN55876.1 hypothetical protein Csa_010555 [Cucumis sativus])

HSP 1 Score: 355.9 bits (912), Expect = 2.9e-94
Identity = 182/252 (72.22%), Postives = 214/252 (84.92%), Query Frame = 0

Query: 7   SSINGETRRSTNFPPNTWGDYFLSNTFETMEIMEHKIEEEVEELKEEVRKLLITCVEKPS 66
           SS+NG+ RRS NFPPN WGDYFLS+TFETM+ ME ++E++VEE+ EEVRK+LITCVEKP 
Sbjct: 5   SSMNGDGRRSANFPPNIWGDYFLSSTFETMK-MEDEVEKKVEEM-EEVRKMLITCVEKPL 64

Query: 67  QQLSLIDSIQRLGVAYHFDTEINEALDHVYNNY-NVITKESDEQIHDVALRFRLLRQQRY 126
           +QL  ID IQRLG+AYHFD EI EALD VY NY N IT ES+E IH V+LRFRLLRQQRY
Sbjct: 65  EQLIFIDLIQRLGLAYHFDIEIKEALDRVYMNYNNRITSESNEDIHTVSLRFRLLRQQRY 124

Query: 127 SISCDIFDKFTDNEGNFKESLAKDVRGILSLYEASHIRVHGEDVLERALAFTTTNLKIAI 186
           SISCDIF+KFT++EGNFKE+L KDV+G+LSLYEA H+R+HGE++LERA AFTTTNL+  I
Sbjct: 125 SISCDIFNKFTNDEGNFKETLTKDVKGMLSLYEACHMRMHGEEILERAFAFTTTNLRATI 184

Query: 187 SASHEGSCGLAAQVSYALKWPLFKAMPRLAAIHYISFYEEDPLHHPALLSFAKLDYNCLQ 246
           + S  G      ++SYALKWP FKAMPRLA+ +YIS YEEDPLH+P LL FAKLDYNCLQ
Sbjct: 185 NNSKGGH--EDKEISYALKWPFFKAMPRLASRNYISSYEEDPLHNPTLLCFAKLDYNCLQ 244

Query: 247 KLYQKEIHEISR 258
           KLYQKE+HEISR
Sbjct: 245 KLYQKELHEISR 252

BLAST of Spg015821 vs. ExPASy Swiss-Prot
Match: Q6Q3H3 ((-)-germacrene D synthase OS=Vitis vinifera OX=29760 GN=VIT_19s0014g04930 PE=1 SV=1)

HSP 1 Score: 256.9 bits (655), Expect = 2.4e-67
Identity = 135/243 (55.56%), Postives = 181/243 (74.49%), Query Frame = 0

Query: 14  RRSTNFPPNTWGDYFLSNTFETMEIMEHKIEEEVEELKEEVRKLLITCVEKPSQQLSLID 73
           RR  NF P+ WGD+FLS   E     +H +++ V++LKEEVRK+L+   +  +Q+L LID
Sbjct: 22  RRCANFHPSIWGDHFLSYASEFTNTDDH-LKQHVQQLKEEVRKMLMAADDDSAQKLLLID 81

Query: 74  SIQRLGVAYHFDTEINEALDHVYNNYNVITKESDEQIHDVALRFRLLRQQRYSISCDIFD 133
           +IQRLGVAYHF++EI+E L H+++  +V++ E D  ++  +LRFRLLRQQ Y +SCD+F+
Sbjct: 82  AIQRLGVAYHFESEIDEVLKHMFDG-SVVSAEED--VYTASLRFRLLRQQGYHVSCDLFN 141

Query: 134 KFTDNEGNFKESLAKDVRGILSLYEASHIRVHGEDVLERALAFTTTNLKIAISASHEGSC 193
            F DNEGNFKESL+ DVRG+LSLYEA+H RVHGED+L+ ALAFTTT+L+   SA+   S 
Sbjct: 142 NFKDNEGNFKESLSSDVRGMLSLYEATHFRVHGEDILDEALAFTTTHLQ---SATKHSSN 201

Query: 194 GLAAQVSYALKWPLFKAMPRLAAIHYISFYEEDPLHHPALLSFAKLDYNCLQKLYQKEIH 253
            LA QV +ALK P+ K +PRL A HY S Y+ D  H+ ALL  AKLD+N LQKL+QKE+ 
Sbjct: 202 PLAEQVVHALKQPIRKGLPRLEARHYFSVYQADDSHNKALLKLAKLDFNLLQKLHQKELS 257

Query: 254 EIS 257
           +IS
Sbjct: 262 DIS 257

BLAST of Spg015821 vs. ExPASy Swiss-Prot
Match: B2KSJ5 ((+)-gamma-cadinene synthase OS=Cucumis melo OX=3656 PE=1 SV=1)

HSP 1 Score: 237.7 bits (605), Expect = 1.5e-61
Identity = 132/266 (49.62%), Postives = 181/266 (68.05%), Query Frame = 0

Query: 3   SQFPSSIN-----GETRRS-TNFPPNTWGDYFLSNTFETMEIMEHKIEEEVEELKEEVRK 62
           S FP+SI       + +RS  NF PN W ++FLS TF+    ++  ++E  E+LKEE+R 
Sbjct: 6   SNFPASIMKTNDIPDVKRSLANFHPNIWKEHFLSFTFDDALKIDEGMKERTEKLKEEIRM 65

Query: 63  LLITCVEKPSQQLSLIDSIQRLGVAYHFDTEINEALDHVYNNYN----VITKESD-EQIH 122
           ++I  VE    +L+L+DSIQRLGV+YHF+ E++E L+H+Y +YN    +  K S+ E +H
Sbjct: 66  MMIAYVENQLIKLNLVDSIQRLGVSYHFEDEVDEFLEHIYVSYNNSLLLSNKNSNGEDLH 125

Query: 123 DVALRFRLLRQQRYSISCDIFDKFTDNEGNFKESLAKDVRGILSLYEASHIRVHGEDVLE 182
             AL FRLLRQQ Y ISCDIF KF D+ G FKESL +D RG+LSLYEASH+  HGE +LE
Sbjct: 126 ITALLFRLLRQQGYRISCDIFLKFMDDNGKFKESLVEDERGLLSLYEASHMMGHGEALLE 185

Query: 183 RALAFTTTNLKIAISASHEGSCGLAAQVSYALKWPLFKAMPRLAAIHYISFYEEDPLHHP 242
            AL FTTT+L+  I      +   A++VS ALK P+ K++PR+ A  Y+  Y++ P H+ 
Sbjct: 186 EALEFTTTHLQTYIHRYSNINPSFASEVSNALKLPIRKSVPRIKAREYLEIYQQHPSHNE 245

Query: 243 ALLSFAKLDYNCLQKLYQKEIHEISR 258
            LL F+KLD+N LQKL+QKE+ EI R
Sbjct: 246 TLLEFSKLDFNILQKLHQKELSEICR 271

BLAST of Spg015821 vs. ExPASy Swiss-Prot
Match: B9SCB6 (Probable terpene synthase 2 OS=Ricinus communis OX=3988 GN=TPS2 PE=3 SV=1)

HSP 1 Score: 231.5 bits (589), Expect = 1.1e-59
Identity = 128/244 (52.46%), Postives = 173/244 (70.90%), Query Frame = 0

Query: 13  TRRSTNFPPNTWGDYFLSNTFETMEIMEHKIEEEVEELKEEVRKLLITCVEKPSQQLSLI 72
           TR   +F PN WGD FLS   E+ +I E  +E++VE LKEEV+K +I   ++PSQ L+LI
Sbjct: 16  TRHLASFHPNIWGDRFLSCAAESTDI-EDDMEQQVERLKEEVKK-MIASADEPSQILNLI 75

Query: 73  DSIQRLGVAYHFDTEINEALDHVYNNYNVITKESDEQIHDVALRFRLLRQQRYSISCDIF 132
           D +QRLGV+YHF+ EI EAL  V N  +    + D+ +H VALRFRLLR+Q  ++SCD+F
Sbjct: 76  DLLQRLGVSYHFEKEIEEALQQVLNMNS--DSDKDDDLHSVALRFRLLREQGLNVSCDVF 135

Query: 133 DKFTDNEGNFKESLAKDVRGILSLYEASHIRVHGEDVLERALAFTTTNLKIAISASHEGS 192
           +KF D  G+F ++L  D++G+LSLYEA+H RVHGE +L+ ALAFTTT L+   S     S
Sbjct: 136 NKFRDRNGHFIQTLKTDLQGMLSLYEAAHFRVHGEGILDDALAFTTTYLE---SIVPNLS 195

Query: 193 CGLAAQVSYALKWPLFKAMPRLAAIHYISFYEEDPLHHPALLSFAKLDYNCLQKLYQKEI 252
             LAAQ+S  L+ PL K++ R+ A H+IS Y+ED  H+  LL+FAKLD+N LQKL+QKE+
Sbjct: 196 PPLAAQISRTLRQPLRKSLARVEARHFISIYQEDTSHNEVLLTFAKLDFNLLQKLHQKEL 252

Query: 253 HEIS 257
             IS
Sbjct: 256 KYIS 252

BLAST of Spg015821 vs. ExPASy Swiss-Prot
Match: Q6Q3H2 (Valencene synthase OS=Vitis vinifera OX=29760 GN=ValCS PE=1 SV=1)

HSP 1 Score: 229.6 bits (584), Expect = 4.1e-59
Identity = 124/247 (50.20%), Postives = 170/247 (68.83%), Query Frame = 0

Query: 12  ETRRSTNFPPNTWGDYFLSNTFETMEIMEHKIEEEVEELKEEV-RKLLITCVEKPSQQLS 71
           + R   NF PN WGD F++ T E       K EE++E+LK+EV RKL    V  PSQ L+
Sbjct: 17  KNRPVANFHPNIWGDQFITYTPEDKVTRACK-EEQIEDLKKEVKRKLTAAAVANPSQLLN 76

Query: 72  LIDSIQRLGVAYHFDTEINEALDHVYNNYNVITKESDEQIHDVALRFRLLRQQRYSISCD 131
            ID++QRLGVAYHF+ EI EAL H+ N+++    + D  ++++AL FRLLRQQ Y+ISCD
Sbjct: 77  FIDAVQRLGVAYHFEQEIEEALQHICNSFH-DCNDMDGDLYNIALGFRLLRQQGYTISCD 136

Query: 132 IFDKFTDNEGNFKESLAKDVRGILSLYEASHIRVHGEDVLERALAFTTTNLKIAISASHE 191
           IF+KFTD  G FKE+L  DVRG+L LYEA+H+RVHGED+L +ALAFTTT+LK  + +   
Sbjct: 137 IFNKFTDERGRFKEALISDVRGMLGLYEAAHLRVHGEDILAKALAFTTTHLKAMVESL-- 196

Query: 192 GSCGLAAQVSYALKWPLFKAMPRLAAIHYISFYEEDPLHHPALLSFAKLDYNCLQKLYQK 251
               LA QV++AL  P+ K + RL A  YIS Y+++  H   LL  AKLD+N +Q L+++
Sbjct: 197 -GYHLAEQVAHALNRPIRKGLERLEARWYISVYQDEAFHDKTLLELAKLDFNLVQSLHKE 256

Query: 252 EIHEISR 258
           E+  ++R
Sbjct: 257 ELSNLAR 258

BLAST of Spg015821 vs. ExPASy Swiss-Prot
Match: F6M8H4 (Probable sesquiterpene synthase OS=Santalum album OX=35974 GN=SesquiTPS1 PE=3 SV=1)

HSP 1 Score: 224.9 bits (572), Expect = 1.0e-57
Identity = 118/247 (47.77%), Postives = 166/247 (67.21%), Query Frame = 0

Query: 13  TRRSTNFPPNTWGDYFLSNTFETMEIMEHKIEEEVEELKEEVRKLLITCVEKPSQQLSLI 72
           +R   NFPP+ WGD F++ T +       K E +VEELKE+VR+ L   V+KP QQ ++I
Sbjct: 18  SRPIANFPPSIWGDRFINYTCQDENERAQK-ERQVEELKEQVRRELAATVDKPLQQQNII 77

Query: 73  DSIQRLGVAYHFDTEINEALDHVYNNYNVITK--ESDEQIHDVALRFRLLRQQRYSISCD 132
           D+ QRLG+AYHF+ EI E+L+H+Y +  V     E  + ++ VAL FRLLRQ  Y +SCD
Sbjct: 78  DATQRLGIAYHFENEIEESLEHIYLHTYVENTCFEGSDDLYSVALWFRLLRQNGYRVSCD 137

Query: 133 IFDKFTDNEGNFKESLAKDVRGILSLYEASHIRVHGEDVLERALAFTTTNLKIAISASHE 192
           +F+KF DNEGNFK +L +D +G+L LYEA+H+ VHGE++L+ AL FT T L+  +S    
Sbjct: 138 VFNKFRDNEGNFKNNLMEDAKGLLELYEATHLSVHGEEMLDDALEFTKTRLESVVS---H 197

Query: 193 GSCGLAAQVSYALKWPLFKAMPRLAAIHYISFYEEDPLHHPALLSFAKLDYNCLQKLYQK 252
            +  LA QV +AL  PL K +PRL A+++   YE    H+ ALL  AKLD+N LQ  ++K
Sbjct: 198 LNYPLAEQVRHALYQPLHKGLPRLEAVYFFRIYEAHASHNKALLKLAKLDFNLLQSFHKK 257

Query: 253 EIHEISR 258
           E+ +I+R
Sbjct: 258 ELSDIAR 260

BLAST of Spg015821 vs. ExPASy TrEMBL
Match: A0A6J1DIG3 ((+)-gamma-cadinene synthase-like OS=Momordica charantia OX=3673 GN=LOC111021378 PE=4 SV=1)

HSP 1 Score: 409.5 bits (1051), Expect = 1.1e-110
Identity = 204/255 (80.00%), Postives = 227/255 (89.02%), Query Frame = 0

Query: 4   QFPSSINGETRRSTNFPPNTWGDYFLSNTFETMEIMEHKIEEEVEELKEEVRKLLITCVE 63
           Q  SSING+TRRS NFPPN WGDYFL NTF +ME ME  IEE+VE +KEEVRKLLITCVE
Sbjct: 13  QSASSINGDTRRSANFPPNIWGDYFLFNTFHSME-MEDNIEEKVEAVKEEVRKLLITCVE 72

Query: 64  KPSQQLSLIDSIQRLGVAYHFDTEINEALDHVYNNYNVITKESDEQIHDVALRFRLLRQQ 123
           KPSQQLSLIDSIQRLG+AYHFDTEINEALDHVY NY+V+  E++E +  VA++FRLLRQQ
Sbjct: 73  KPSQQLSLIDSIQRLGIAYHFDTEINEALDHVYKNYSVVCNENNEDLQIVAMKFRLLRQQ 132

Query: 124 RYSISCDIFDKFTDNEGNFKESLAKDVRGILSLYEASHIRVHGEDVLERALAFTTTNLKI 183
           RYSISCDIFDKFTD+EGNFKESLAKDVRG+LSLYEASH+R+HGEDVLERAL+F+TTNLK 
Sbjct: 133 RYSISCDIFDKFTDDEGNFKESLAKDVRGMLSLYEASHVRIHGEDVLERALSFSTTNLKA 192

Query: 184 AIS-ASHEGSCGLAAQVSYALKWPLFKAMPRLAAIHYISFYEEDPLHHPALLSFAKLDYN 243
           AIS  SHEGSCG+  QV +ALKWP+FKAMPRLAA  YISFYE+DPLHHP LL  AKLDYN
Sbjct: 193 AISNGSHEGSCGIGGQVRHALKWPVFKAMPRLAARSYISFYEQDPLHHPTLLYLAKLDYN 252

Query: 244 CLQKLYQKEIHEISR 258
            LQKLYQKE+HEISR
Sbjct: 253 ILQKLYQKELHEISR 266

BLAST of Spg015821 vs. ExPASy TrEMBL
Match: A0A6J1DKT6 ((-)-germacrene D synthase-like OS=Momordica charantia OX=3673 GN=LOC111021377 PE=4 SV=1)

HSP 1 Score: 403.7 bits (1036), Expect = 5.9e-109
Identity = 202/255 (79.22%), Postives = 228/255 (89.41%), Query Frame = 0

Query: 4   QFPSSINGETRRSTNFPPNTWGDYFLSNTFETMEIMEHKIEEEVEELKEEVRKLLITCVE 63
           Q  SS+NG+TRRS NFPPN WGDYFLSN F +M+ ME  IEE+VE LKE VRKLLITCVE
Sbjct: 13  QSASSVNGDTRRSANFPPNIWGDYFLSNIFHSMD-MEDNIEEKVEALKEGVRKLLITCVE 72

Query: 64  KPSQQLSLIDSIQRLGVAYHFDTEINEALDHVYNNYNVITKESDEQIHDVALRFRLLRQQ 123
           KPSQQLSLIDSIQRLG+AYHFDTEINEAL+HVY NY V++ +S+E +  VAL+FRLLRQQ
Sbjct: 73  KPSQQLSLIDSIQRLGIAYHFDTEINEALNHVYKNYTVVSNKSNEDLQIVALKFRLLRQQ 132

Query: 124 RYSISCDIFDKFTDNEGNFKESLAKDVRGILSLYEASHIRVHGEDVLERALAFTTTNLKI 183
           RYSISC+IFDKFTD+EGNFKESLAKDVRG+LSLYEASH+R+HGEDVLERALAF+TTNLK 
Sbjct: 133 RYSISCEIFDKFTDDEGNFKESLAKDVRGMLSLYEASHVRMHGEDVLERALAFSTTNLKA 192

Query: 184 AIS-ASHEGSCGLAAQVSYALKWPLFKAMPRLAAIHYISFYEEDPLHHPALLSFAKLDYN 243
           AIS  SHEGS G  AQV +ALKWP+FKAMPRLAA +YISFYE+DPLHHP LLSFAKLDYN
Sbjct: 193 AISNGSHEGSWGFGAQVRHALKWPVFKAMPRLAARNYISFYEQDPLHHPTLLSFAKLDYN 252

Query: 244 CLQKLYQKEIHEISR 258
            LQKLYQ+E+HEI+R
Sbjct: 253 TLQKLYQRELHEITR 266

BLAST of Spg015821 vs. ExPASy TrEMBL
Match: A0A6J1DKI4 ((-)-germacrene D synthase-like OS=Momordica charantia OX=3673 GN=LOC111021373 PE=4 SV=1)

HSP 1 Score: 383.6 bits (984), Expect = 6.3e-103
Identity = 193/252 (76.59%), Postives = 219/252 (86.90%), Query Frame = 0

Query: 7   SSINGETRRSTNFPPNTWGDYFLSNTFETMEIMEHKIEEEVEELKEEVRKLLITCVEKPS 66
           S ING+TRRS NFPPN WGDYFLS TF + EI E  IEE+VE L+EEVRKLL + VEK S
Sbjct: 16  SPINGDTRRSANFPPNIWGDYFLSGTFRSTEI-EDNIEEKVEALEEEVRKLLFSYVEKSS 75

Query: 67  QQLSLIDSIQRLGVAYHFDTEINEALDHVYNNYNVITKESDEQIHDVALRFRLLRQQRYS 126
           QQLSLIDSIQRLG+AYHFDTEINEALDHVY NY+ ++ ES + +H VAL+F LLRQQRYS
Sbjct: 76  QQLSLIDSIQRLGIAYHFDTEINEALDHVYKNYSFVSNESQKDLHIVALKFHLLRQQRYS 135

Query: 127 ISCDIFDKFTDNEGNFKESLAKDVRGILSLYEASHIRVHGEDVLERALAFTTTNLKIAI- 186
           ISC+IFDKFT++EGNFKESLA +VRG+LSLYEASH+R+HGEDVLERAL F+T NL+ AI 
Sbjct: 136 ISCEIFDKFTNDEGNFKESLANEVRGMLSLYEASHMRMHGEDVLERALTFSTINLEAAIN 195

Query: 187 SASHEGSCGLAAQVSYALKWPLFKAMPRLAAIHYISFYEEDPLHHPALLSFAKLDYNCLQ 246
           + SHEGSCG  AQV +ALKWP+FKAMPRLAA  YISFYEEDPLHHPALLS AKLD+N LQ
Sbjct: 196 NGSHEGSCGFGAQVRHALKWPVFKAMPRLAARSYISFYEEDPLHHPALLSMAKLDFNILQ 255

Query: 247 KLYQKEIHEISR 258
           KLYQKE+HEISR
Sbjct: 256 KLYQKELHEISR 266

BLAST of Spg015821 vs. ExPASy TrEMBL
Match: A0A1S3BLL1 ((-)-germacrene D synthase-like OS=Cucumis melo OX=3656 GN=LOC103490898 PE=4 SV=1)

HSP 1 Score: 371.3 bits (952), Expect = 3.2e-99
Identity = 187/254 (73.62%), Postives = 221/254 (87.01%), Query Frame = 0

Query: 8   SINGETRRSTNFPPNTWGDYFLSNTFETMEIMEHKIEEEVEELKEEVRKLLITCVEKPSQ 67
           SING+ RRS NFPPN WGDYFLSNTF+TME ME+++E++VEE+KEEVRK+LITCVEKP +
Sbjct: 19  SINGDARRSANFPPNIWGDYFLSNTFQTME-MENEVEKKVEEMKEEVRKMLITCVEKPFE 78

Query: 68  QLSLIDSIQRLGVAYHFDTEINEALDHVYNNY-NVITKESDEQIHDVALRFRLLRQQRYS 127
           QL LIDSIQ+LG+AYHF+ EI EALD VY NY N+IT ES+E IH V+LRFRLLRQQRYS
Sbjct: 79  QLILIDSIQQLGLAYHFEIEIKEALDDVYMNYNNLITSESNEDIHIVSLRFRLLRQQRYS 138

Query: 128 ISCDIFDKFTDNEGNFKESLAKDVRGILSLYEASHIRVHGEDVLERALAFTTTNLKIAI- 187
           ISCDIF+KFTD+EGNFKE+L KDV+G+LSLY+ASH+R+HGE++LERA AFTTTNL+ AI 
Sbjct: 139 ISCDIFNKFTDDEGNFKETLTKDVKGMLSLYQASHMRMHGEEILERAFAFTTTNLRAAIN 198

Query: 188 --SASHEGSCGLAAQVSYALKWPLFKAMPRLAAIHYISFYEEDPLHHPALLSFAKLDYNC 247
             + SHE       ++SYALKWP FKAMPRLA+ +YIS YEEDPLH+P LL FAKLDYNC
Sbjct: 199 NSNGSHED-----REISYALKWPFFKAMPRLASRNYISSYEEDPLHNPTLLCFAKLDYNC 258

Query: 248 LQKLYQKEIHEISR 258
           LQKLYQKE+HEISR
Sbjct: 259 LQKLYQKELHEISR 266

BLAST of Spg015821 vs. ExPASy TrEMBL
Match: A0A0A0L6X1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G021130 PE=4 SV=1)

HSP 1 Score: 355.9 bits (912), Expect = 1.4e-94
Identity = 182/252 (72.22%), Postives = 214/252 (84.92%), Query Frame = 0

Query: 7   SSINGETRRSTNFPPNTWGDYFLSNTFETMEIMEHKIEEEVEELKEEVRKLLITCVEKPS 66
           SS+NG+ RRS NFPPN WGDYFLS+TFETM+ ME ++E++VEE+ EEVRK+LITCVEKP 
Sbjct: 5   SSMNGDGRRSANFPPNIWGDYFLSSTFETMK-MEDEVEKKVEEM-EEVRKMLITCVEKPL 64

Query: 67  QQLSLIDSIQRLGVAYHFDTEINEALDHVYNNY-NVITKESDEQIHDVALRFRLLRQQRY 126
           +QL  ID IQRLG+AYHFD EI EALD VY NY N IT ES+E IH V+LRFRLLRQQRY
Sbjct: 65  EQLIFIDLIQRLGLAYHFDIEIKEALDRVYMNYNNRITSESNEDIHTVSLRFRLLRQQRY 124

Query: 127 SISCDIFDKFTDNEGNFKESLAKDVRGILSLYEASHIRVHGEDVLERALAFTTTNLKIAI 186
           SISCDIF+KFT++EGNFKE+L KDV+G+LSLYEA H+R+HGE++LERA AFTTTNL+  I
Sbjct: 125 SISCDIFNKFTNDEGNFKETLTKDVKGMLSLYEACHMRMHGEEILERAFAFTTTNLRATI 184

Query: 187 SASHEGSCGLAAQVSYALKWPLFKAMPRLAAIHYISFYEEDPLHHPALLSFAKLDYNCLQ 246
           + S  G      ++SYALKWP FKAMPRLA+ +YIS YEEDPLH+P LL FAKLDYNCLQ
Sbjct: 185 NNSKGGH--EDKEISYALKWPFFKAMPRLASRNYISSYEEDPLHNPTLLCFAKLDYNCLQ 244

Query: 247 KLYQKEIHEISR 258
           KLYQKE+HEISR
Sbjct: 245 KLYQKELHEISR 252

BLAST of Spg015821 vs. TAIR 10
Match: AT3G14540.1 (Terpenoid cyclases/Protein prenyltransferases superfamily protein )

HSP 1 Score: 155.2 bits (391), Expect = 7.0e-38
Identity = 87/246 (35.37%), Postives = 144/246 (58.54%), Query Frame = 0

Query: 13  TRRSTNFPPNTWGDYFLSNTFETMEIMEHKIEEEVEELKEEVRKLLITCVEKPSQQLSLI 72
           +R ST F P+ WGD+FLS + +  E +E  +E E+E +K  V+ +L++      +++ LI
Sbjct: 59  SRPSTYFSPSLWGDHFLSVSLDHAEFVE--LEREIETMKPLVKDMLMSSQSSDKEKIRLI 118

Query: 73  DSIQRLGVAYHFDTEINEALDHVYNNYNVITKESDEQIHDVALRFRLLRQQRYSISCDIF 132
             +  LG +YHFD EI + L H +   + I  E D+ +  +++ F + R   + +SCD F
Sbjct: 119 HLLVSLGSSYHFDKEIQDILKHSFTKLDGIIVEEDD-LETISIMFEVFRLYGHKMSCDAF 178

Query: 133 DKFTDNEGNFKESLAKDVRGILSLYEASHIRVHGEDVLERALAFTTTNLKIAISAS-HEG 192
           D+F   +G FKESLAKDVRG+L L+E +H+    ED+++ AL FT  +L+   S +    
Sbjct: 179 DRFRGGDGRFKESLAKDVRGMLQLFEVAHLGTLSEDIMDEALRFTRNHLESLTSGNVSSA 238

Query: 193 SCGLAAQVSYALKWPLFKAMPRLAAIHYISFYEEDPLHHPALLSFAKLDYNCLQKLYQKE 252
           S  +   +  +L  P +  +  L A  YIS+YE++  ++  LL FAKL++N  Q  Y +E
Sbjct: 239 SPHILKHIQNSLYIPRYCNIEVLVAREYISYYEQEEGYNEILLKFAKLNFNFCQCHYIQE 298

Query: 253 IHEISR 258
           I  +++
Sbjct: 299 IKTLTK 301

BLAST of Spg015821 vs. TAIR 10
Match: AT5G23960.1 (terpene synthase 21 )

HSP 1 Score: 154.5 bits (389), Expect = 1.2e-37
Identity = 87/246 (35.37%), Postives = 142/246 (57.72%), Query Frame = 0

Query: 14  RRSTNFPPNTWGDYFLSNTFETMEIMEHKIEEEVEELKEEVRKLLITCVEKPSQQLSLID 73
           R   +FP N W D   S  F   ++     +E+   LKE V++  ++    P + +  ID
Sbjct: 7   RPLADFPANIWEDPLTS--FSKSDLGTETFKEKHSTLKEAVKEAFMSSKANPIENIKFID 66

Query: 74  SIQRLGVAYHFDTEINEALDHVYN--NYNVITKESDEQIHDVALRFRLLRQQRYSISCDI 133
           ++ RLGV+YHF+ +I E LD  ++  ++  + ++    ++ V + F++ RQ  + +S D+
Sbjct: 67  ALCRLGVSYHFEKDIVEQLDKSFDCLDFPQMVRQEGCDLYTVGIIFQVFRQFGFKLSADV 126

Query: 134 FDKFTDNEGNFKESLAKDVRGILSLYEASHIRVHGEDVLERALAFTTTNLKIAISASHEG 193
           F+KF D  G FK  L  D  G+LSLYEA+    HGED+++ ALAF+ ++L+     S   
Sbjct: 127 FEKFKDENGKFKGHLVTDAYGMLSLYEAAQWGTHGEDIIDEALAFSRSHLE---EISSRS 186

Query: 194 SCGLAAQVSYALKWPLFKAMPRLAAIHYISFYEEDPLHHPALLSFAKLDYNCLQKLYQKE 253
           S  LA ++  ALK P  K + R+    YIS+YEE+    P LL FAK+D+N LQ L+++E
Sbjct: 187 SPHLAIRIKNALKHPYHKGISRIETRQYISYYEEEESCDPTLLEFAKIDFNLLQILHREE 246

Query: 254 IHEISR 258
           +  ++R
Sbjct: 247 LACVTR 247

BLAST of Spg015821 vs. TAIR 10
Match: AT5G23960.2 (terpene synthase 21 )

HSP 1 Score: 154.5 bits (389), Expect = 1.2e-37
Identity = 87/246 (35.37%), Postives = 142/246 (57.72%), Query Frame = 0

Query: 14  RRSTNFPPNTWGDYFLSNTFETMEIMEHKIEEEVEELKEEVRKLLITCVEKPSQQLSLID 73
           R   +FP N W D   S  F   ++     +E+   LKE V++  ++    P + +  ID
Sbjct: 7   RPLADFPANIWEDPLTS--FSKSDLGTETFKEKHSTLKEAVKEAFMSSKANPIENIKFID 66

Query: 74  SIQRLGVAYHFDTEINEALDHVYN--NYNVITKESDEQIHDVALRFRLLRQQRYSISCDI 133
           ++ RLGV+YHF+ +I E LD  ++  ++  + ++    ++ V + F++ RQ  + +S D+
Sbjct: 67  ALCRLGVSYHFEKDIVEQLDKSFDCLDFPQMVRQEGCDLYTVGIIFQVFRQFGFKLSADV 126

Query: 134 FDKFTDNEGNFKESLAKDVRGILSLYEASHIRVHGEDVLERALAFTTTNLKIAISASHEG 193
           F+KF D  G FK  L  D  G+LSLYEA+    HGED+++ ALAF+ ++L+     S   
Sbjct: 127 FEKFKDENGKFKGHLVTDAYGMLSLYEAAQWGTHGEDIIDEALAFSRSHLE---EISSRS 186

Query: 194 SCGLAAQVSYALKWPLFKAMPRLAAIHYISFYEEDPLHHPALLSFAKLDYNCLQKLYQKE 253
           S  LA ++  ALK P  K + R+    YIS+YEE+    P LL FAK+D+N LQ L+++E
Sbjct: 187 SPHLAIRIKNALKHPYHKGISRIETRQYISYYEEEESCDPTLLEFAKIDFNLLQILHREE 246

Query: 254 IHEISR 258
           +  ++R
Sbjct: 247 LACVTR 247

BLAST of Spg015821 vs. TAIR 10
Match: AT3G14490.1 (Terpenoid cyclases/Protein prenyltransferases superfamily protein )

HSP 1 Score: 149.8 bits (377), Expect = 2.9e-36
Identity = 86/248 (34.68%), Postives = 145/248 (58.47%), Query Frame = 0

Query: 13  TRRSTNFPPNTWGDYFLSNTFETMEIMEHKIEEEVEELKEEVRKLLITCVEKPSQQLSLI 72
           +R   +F P  WGD+FLS      E  +   E EV  +K +VR +L +     ++++ LI
Sbjct: 59  SRPLAHFSPTLWGDHFLSVPLHVAEFDDFSREIEV-TMKPKVRDMLKSSKNSDNERIRLI 118

Query: 73  DSIQRLGVAYHFDTEINEALDHVYNNY-NVITKESDEQIHDVALRFRLLRQQRYSISCDI 132
             +  LG+AYHF+ EI+E L   + N  ++I KE+D  +  ++  F + R + Y + C  
Sbjct: 119 HLLMNLGIAYHFEIEIDEILGQAFGNLDDIIAKEND--LETISTMFEVFRLRGYYMPCYA 178

Query: 133 FDKFTDNEGNFKESLAKDVRGILSLYEASHIRVHGEDVLERALAFTTTNLKIAISASHEG 192
           F++F   +G FKESLA+D+RG+L LYEA+H+    ED+++ AL+FT   L+ +++++H  
Sbjct: 179 FNRFKGEDGRFKESLAEDIRGMLQLYEAAHLGTPSEDIMDEALSFTRYRLE-SLTSNHTA 238

Query: 193 SCG--LAAQVSYALKWPLFKAMPRLAAIHYISFYEEDPLHHPALLSFAKLDYNCLQKLYQ 252
           +    L+  +  AL    +  +  L A  YISFYE++  H   LL FAKL++N  Q  Y 
Sbjct: 239 TASPHLSKHIQNALYRARYHNLEILVAREYISFYEQEEDHDETLLKFAKLNFNYCQLHYI 298

Query: 253 KEIHEISR 258
           +E+ ++++
Sbjct: 299 QELKDLTK 302

BLAST of Spg015821 vs. TAIR 10
Match: AT3G14520.1 (Terpenoid cyclases/Protein prenyltransferases superfamily protein )

HSP 1 Score: 149.8 bits (377), Expect = 2.9e-36
Identity = 85/246 (34.55%), Postives = 141/246 (57.32%), Query Frame = 0

Query: 13  TRRSTNFPPNTWGDYFLSNTFETMEIMEHKIEEEVEELKEEVRKLLITCVEKPSQQLSLI 72
           +R ST F P+ WGD+FLS + +  E  E  +E E+E +K  V+ +L++      +++ LI
Sbjct: 62  SRPSTYFSPSLWGDHFLSVSLDRGEFDE--LEREIETMKPLVKDMLMSSQSSDKEKIRLI 121

Query: 73  DSIQRLGVAYHFDTEINEALDHVYNNYNVITKESDEQIHDVALRFRLLRQQRYSISCDIF 132
             +  LG +YHFD EI + L H +   + I    D+ +  +++ F + R   + +SCD F
Sbjct: 122 HLLVSLGSSYHFDKEIQDILKHSFTKLDDIIVGEDD-LETISIMFEVFRLYGHKMSCDAF 181

Query: 133 DKFTDNEGNFKESLAKDVRGILSLYEASHIRVHGEDVLERALAFTTTNLKIAISASHEGS 192
           D+F   +G FKESLAKDVRG+L L+E +H+    ED+++ A +F   +L   I  +  G+
Sbjct: 182 DRFRGEDGRFKESLAKDVRGMLQLFEVAHLGTPSEDIMDEASSFAQNHLDSWIGGNVSGA 241

Query: 193 C-GLAAQVSYALKWPLFKAMPRLAAIHYISFYEEDPLHHPALLSFAKLDYNCLQKLYQKE 252
              L   +  +L  P +  +  L A  YIS+YE++  H+  LL FAKL++N  Q  Y +E
Sbjct: 242 TPHLLKHIQNSLYIPRYCNIEVLVAREYISYYEQEEGHNKILLKFAKLNFNFCQFHYIQE 301

Query: 253 IHEISR 258
           +  +++
Sbjct: 302 LKTLTK 304

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022154020.12.2e-11080.00(+)-gamma-cadinene synthase-like [Momordica charantia][more]
XP_022154019.11.2e-10879.22(-)-germacrene D synthase-like [Momordica charantia][more]
XP_022154012.11.3e-10276.59(-)-germacrene D synthase-like [Momordica charantia][more]
XP_008448865.16.7e-9973.62PREDICTED: (-)-germacrene D synthase-like [Cucumis melo][more]
XP_004147250.22.9e-9472.22(-)-germacrene D synthase [Cucumis sativus] >KGN55876.1 hypothetical protein Csa... [more]
Match NameE-valueIdentityDescription
Q6Q3H32.4e-6755.56(-)-germacrene D synthase OS=Vitis vinifera OX=29760 GN=VIT_19s0014g04930 PE=1 S... [more]
B2KSJ51.5e-6149.62(+)-gamma-cadinene synthase OS=Cucumis melo OX=3656 PE=1 SV=1[more]
B9SCB61.1e-5952.46Probable terpene synthase 2 OS=Ricinus communis OX=3988 GN=TPS2 PE=3 SV=1[more]
Q6Q3H24.1e-5950.20Valencene synthase OS=Vitis vinifera OX=29760 GN=ValCS PE=1 SV=1[more]
F6M8H41.0e-5747.77Probable sesquiterpene synthase OS=Santalum album OX=35974 GN=SesquiTPS1 PE=3 SV... [more]
Match NameE-valueIdentityDescription
A0A6J1DIG31.1e-11080.00(+)-gamma-cadinene synthase-like OS=Momordica charantia OX=3673 GN=LOC111021378 ... [more]
A0A6J1DKT65.9e-10979.22(-)-germacrene D synthase-like OS=Momordica charantia OX=3673 GN=LOC111021377 PE... [more]
A0A6J1DKI46.3e-10376.59(-)-germacrene D synthase-like OS=Momordica charantia OX=3673 GN=LOC111021373 PE... [more]
A0A1S3BLL13.2e-9973.62(-)-germacrene D synthase-like OS=Cucumis melo OX=3656 GN=LOC103490898 PE=4 SV=1[more]
A0A0A0L6X11.4e-9472.22Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G021130 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT3G14540.17.0e-3835.37Terpenoid cyclases/Protein prenyltransferases superfamily protein [more]
AT5G23960.11.2e-3735.37terpene synthase 21 [more]
AT5G23960.21.2e-3735.37terpene synthase 21 [more]
AT3G14490.12.9e-3634.68Terpenoid cyclases/Protein prenyltransferases superfamily protein [more]
AT3G14520.12.9e-3634.55Terpenoid cyclases/Protein prenyltransferases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 33..60
NoneNo IPR availablePANTHERPTHR31225OS04G0344100 PROTEIN-RELATEDcoord: 12..257
NoneNo IPR availablePANTHERPTHR31225:SF111TERPENE SYNTHASE 2-RELATEDcoord: 12..257
IPR008949Isoprenoid synthase domain superfamilyGENE3D1.10.600.10Farnesyl Diphosphate Synthasecoord: 21..257
e-value: 3.6E-83
score: 281.7
IPR001906Terpene synthase, N-terminal domainPFAMPF01397Terpene_synthcoord: 24..203
e-value: 5.2E-59
score: 199.2
IPR036965Terpene synthase, N-terminal domain superfamilyGENE3D1.50.10.130coord: 63..229
e-value: 3.6E-83
score: 281.7
IPR008930Terpenoid cyclases/protein prenyltransferase alpha-alpha toroidSUPERFAMILY48239Terpenoid cyclases/Protein prenyltransferasescoord: 20..227

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg015821.1Spg015821.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016102 diterpenoid biosynthetic process
molecular_function GO:0000287 magnesium ion binding
molecular_function GO:0010333 terpene synthase activity
molecular_function GO:0016829 lyase activity