Spg015728 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg015728
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	tRNA (Guanosine(18)-2'-O)-methyltransferase
Location	scaffold10: 19692013 .. 19695086 (-)
RNA-Seq Expression	Spg015728
Synteny	Spg015728

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TTTCTGTTTTACTCGCCCCACTTTCTTCTACCGCTGTCTGAACCATCGAATCTCTGCAAGCAACGCGCAGCCATGGCGTCCTGCAGAATCGCAGCCCGTTCTTCTCTTATTCTGCTGGAAAGCTGCAGTCCCCGAAGAACTCAACGAATCCTCCCTTCTGTTTCCCTCCCTTCCCCACCTCTTTCTTCCTTATCCCCATTTTTCACCATCGTCACAAATCAATGTACCGTCCTCTCTCTCTCTTTCTTGGAGGCTATACAGTATTTTTACGTTTCTTTTATAACTCTGTTCTTCAATTTCATTCTGTTTTAATTGCTATATAGCCTCAAGAAACAACCCTAACGCAGTTCCTGCAAGATGTTTTGCTTCAATTTCGGCCGCAACATCTTCAATAGAAGTGGCAGATTCTGCGGGCGACCTAGAACCGCCGGAAATTCATACGCAGGACACGGTGGAGCACCTTCTAACGCACTCCGATGACGTTGCCAGGTTGATGAAGATGGAGCGGAAGCCGATTGCAGATGGAGCTGAATACCCCTGTAAAGGGCGGTGGTTTCCGTACCTCGATAGGTTTAGCTGTGGAAGCTCTTGGTTGAGTAGTGGTGAGATTTTGGAGGCACTGGATCCTCTCATATTGGACTCGAGGAAGCAGCGGTTCAAGAACGTGGTGCGGAACAGGAGCTACTCGGTTTGTTTGGTGGTGGAGGGGCTGAGTGATTTCGGCAACGTCTCGGCGGCATTCCGATCTGCCGATGCGCTTGGATTTCAGTCGGTTCATGTGGTCTCCTGCGATGCTTCCAAAAGGTGCAACAACGATGGAGGTGAAATTGTTTTCGAATACATTTATTTTTTCTTTTAGGAAAATTGTTTGATTCTGGAATTCGGCAGCTGCTTTGTTTCCGTTGTGCTGGCGGGACTTCGTTACTGTTCATGGTGCTTTTACTTTGTTATTATTCAACGGGTCACTTGAATTCAATCAGGCGATTGCCATTGCGGGTTTTATATGGTTAGAAGGTTAAATCGATCACAATACATTTGATTTTCCATGGTAATTGTTAGATATGGGTGACTGAAATTAAGCACTGGAATGGAGTGTTTTCTTCCAGCAAATATGCATCCGTTGTATATGTATTTATATTTTTAACTCTTGTATTGGTTGTATGTATATTGTATATTATCATTGGATACTCTTTGCCAAATATTGATTATAATATATTCTTTTCCATTGCCCAAGGTGTCTCTTCATGTTGGTTTTCCATGTGAAATCTATGTGTTCATATGTTTTGTCTTCCTTTAGTTGCTGTCTTTATCTCGCCATATTTCATGACAGTAAGTGCCGTTTGTCTTGGACATGAAACTTTAAGAGGTGCCTGTATTCTCATGCTTATGCTGAATGAGATGATGCTTTGATGAATTAGGGTGCAACAGTGGTGATATATATTGTCTCTATGGATTATGGAAAGAGCTACTCCTATTTAATGGGTCATTCAACTTACTAAGAATGGCGGAATCTTCCATTTCTTACATCTTCTTTCACGAGTTTTTGTATTTTCTTCTGTCTTCTTCCAACATCATTGCAATTTCAATGCAGGTACAGAGAGAATCGTCATGTTAGCATGGGAGCAGAGAAATGGTTGGACATTGAACTTTGGAACTCCACCCCAGAATGTTTTAAAATTCTGAAATCTCGCGGTTATAGAATAGTCTCTACGCATCTTGGAACGAATGCGGTATCTATCAATTTTGCACTTTCACCCAATTCACAATGAAGTAACTTAACTTTGAGGTCATGTCTGCACTAGCTTTTTTAAGCTTATCTTGATAGCTGAGTTGTGTTTGTCCTGTTGTAGGTTTCTGTCTATGATATGGATTGGTCGTGTCCAACTGCAATTGTGGTTGGAAATGAAAGTAGGTAATTATCTTCTTGCTACAATAATATTGAGTCTGATCAAGTTTGGAGCCCTTTAAATTAAGGAATGACTGCAATAGTTGTTTTTTCTTTGTTATTTGATGCACCTTTTTGAATCACGATTCTCTCATGGTTTGATTAGCAAGTTTCTACTCATGAATGATGTCTGAATCAGGAGAAATTATACTTGTAGCTAGTTATGAACTTTGCAATTTATGTTAGTATCTGAGGTTCAAATTTCTTCTGTCTCAGGGGAATAAGCGATGAAGCCCTTGAGCTATCAGATTTGCATTGCAGTATTCCTATGAATGGCATGGTAGATTCCTTCAACGTATCAGTTGCTGCTGGCATCCTCATGCACCATGCTGTTTGCGACCGGACTTCTCGACTGGTAACTAAAAGAGTCATGTTTGATATTTGAATTCATTTATGCAATAAGCAGGGATTCTGTTGGGGTGTTACTAGGGGTATTCTGTGGAATTATGCCAGTATCTGGATAGCTCAATAAGAGAAAATCCTAGTTGTCTCTCGGAGTTGGCATGTGAGGATACTGAGTCGTGTGGGGTGGGGGCTGATACGAAGGATGTCAAATACTGTTTCTGTTATACGATCAACTATGTTCTTTAACGAACTTTTCAGGGCTATCATGGAGATCTCACATTAGATGAAAGGCAAATTCTACTTGCGGAGTTCTCTTTGCGGCATAGCAAGAATGCAATCAGTATCGCCACCGAGTACGCAAAGAGGAAGGGTACAGAGGAATTGGACTCGAATGGCAAGAATTTGGCCTCGAATGGCAAGAATTTGGCCTCTTGATGTGCCTAGGCCTAGGGACATGACTATGAGATGCAAAAACACACAAGCTCCCTTGAAGGTTGATTCAAACAGGTAAAATTCCTGAGGCCTGTGATGAAACCTAGCTCAGAAAATTTTCCAGCAGTACAATTTCCCCATATGAAGCATTTTATCGGCCTGCTTTCTGTGACAAGCTTCAGCATCAATTCACATGGCTGTATAATGTTATATTGTAGTTCTGTTAAATTGACTTGGATAAACTTTAGTTGGGTCAGCATTAGTAAAATGTCTACCTTTCTCATTCAATGAGAGATCTACATGAGTAGTATATCAATAACTCACTAGAATCATGTTCAAGACAATCAACCAA

mRNA sequence

TTTCTGTTTTACTCGCCCCACTTTCTTCTACCGCTGTCTGAACCATCGAATCTCTGCAAGCAACGCGCAGCCATGGCGTCCTGCAGAATCGCAGCCCGTTCTTCTCTTATTCTGCTGGAAAGCTGCAGTCCCCGAAGAACTCAACGAATCCTCCCTTCTGTTTCCCTCCCTTCCCCACCTCTTTCTTCCTTATCCCCATTTTTCACCATCGTCACAAATCAATCCTCAAGAAACAACCCTAACGCAGTTCCTGCAAGATGTTTTGCTTCAATTTCGGCCGCAACATCTTCAATAGAAGTGGCAGATTCTGCGGGCGACCTAGAACCGCCGGAAATTCATACGCAGGACACGGTGGAGCACCTTCTAACGCACTCCGATGACGTTGCCAGGTTGATGAAGATGGAGCGGAAGCCGATTGCAGATGGAGCTGAATACCCCTGTAAAGGGCGGTGGTTTCCGTACCTCGATAGGTTTAGCTGTGGAAGCTCTTGGTTGAGTAGTGGTGAGATTTTGGAGGCACTGGATCCTCTCATATTGGACTCGAGGAAGCAGCGGTTCAAGAACGTGGTGCGGAACAGGAGCTACTCGGTTTGTTTGGTGGTGGAGGGGCTGAGTGATTTCGGCAACGTCTCGGCGGCATTCCGATCTGCCGATGCGCTTGGATTTCAGTCGGTTCATGTGGTCTCCTGCGATGCTTCCAAAAGGTGCAACAACGATGGAGCTGCTTTGTTTCCGTTGTGCTGGCGGGACTTCGTTACTGTTCATGGTGCTTTTACTTTGTTATTATTCAACGGGTCACTTGAATTCAATCAGGCGATTGCCATTGCGGGTTTTATATGTTTTTGTATTTTCTTCTGTCTTCTTCCAACATCATTGCAATTTCAATGCAGGTACAGAGAGAATCGTCATGTTAGCATGGGAGCAGAGAAATGGTTGGACATTGAACTTTGGAACTCCACCCCAGAATGTTTTAAAATTCTGAAATCTCGCGGTTATAGAATAGTCTCTACGCATCTTGGAACGAATGCGGTTTCTGTCTATGATATGGATTGGTCGTGTCCAACTGCAATTGTGGTTGGAAATGAAAGTAGGGGAATAAGCGATGAAGCCCTTGAGCTATCAGATTTGCATTGCAGTATTCCTATGAATGGCATGGTAGATTCCTTCAACGTATCAGTTGCTGCTGGCATCCTCATGCACCATGCTGTTTGCGACCGGACTTCTCGACTGGGCTATCATGGAGATCTCACATTAGATGAAAGGCAAATTCTACTTGCGGAGTTCTCTTTGCGGCATAGCAAGAATGCAATCAGTATCGCCACCGAGTACGCAAAGAGGAAGGGTACAGAGGAATTGGACTCGAATGGCAAGAATTTGGCCTCGAATGGCAAGAATTTGGCCTCTTGA

Coding sequence (CDS)

TTTCTGTTTTACTCGCCCCACTTTCTTCTACCGCTGTCTGAACCATCGAATCTCTGCAAGCAACGCGCAGCCATGGCGTCCTGCAGAATCGCAGCCCGTTCTTCTCTTATTCTGCTGGAAAGCTGCAGTCCCCGAAGAACTCAACGAATCCTCCCTTCTGTTTCCCTCCCTTCCCCACCTCTTTCTTCCTTATCCCCATTTTTCACCATCGTCACAAATCAATCCTCAAGAAACAACCCTAACGCAGTTCCTGCAAGATGTTTTGCTTCAATTTCGGCCGCAACATCTTCAATAGAAGTGGCAGATTCTGCGGGCGACCTAGAACCGCCGGAAATTCATACGCAGGACACGGTGGAGCACCTTCTAACGCACTCCGATGACGTTGCCAGGTTGATGAAGATGGAGCGGAAGCCGATTGCAGATGGAGCTGAATACCCCTGTAAAGGGCGGTGGTTTCCGTACCTCGATAGGTTTAGCTGTGGAAGCTCTTGGTTGAGTAGTGGTGAGATTTTGGAGGCACTGGATCCTCTCATATTGGACTCGAGGAAGCAGCGGTTCAAGAACGTGGTGCGGAACAGGAGCTACTCGGTTTGTTTGGTGGTGGAGGGGCTGAGTGATTTCGGCAACGTCTCGGCGGCATTCCGATCTGCCGATGCGCTTGGATTTCAGTCGGTTCATGTGGTCTCCTGCGATGCTTCCAAAAGGTGCAACAACGATGGAGCTGCTTTGTTTCCGTTGTGCTGGCGGGACTTCGTTACTGTTCATGGTGCTTTTACTTTGTTATTATTCAACGGGTCACTTGAATTCAATCAGGCGATTGCCATTGCGGGTTTTATATGTTTTTGTATTTTCTTCTGTCTTCTTCCAACATCATTGCAATTTCAATGCAGGTACAGAGAGAATCGTCATGTTAGCATGGGAGCAGAGAAATGGTTGGACATTGAACTTTGGAACTCCACCCCAGAATGTTTTAAAATTCTGAAATCTCGCGGTTATAGAATAGTCTCTACGCATCTTGGAACGAATGCGGTTTCTGTCTATGATATGGATTGGTCGTGTCCAACTGCAATTGTGGTTGGAAATGAAAGTAGGGGAATAAGCGATGAAGCCCTTGAGCTATCAGATTTGCATTGCAGTATTCCTATGAATGGCATGGTAGATTCCTTCAACGTATCAGTTGCTGCTGGCATCCTCATGCACCATGCTGTTTGCGACCGGACTTCTCGACTGGGCTATCATGGAGATCTCACATTAGATGAAAGGCAAATTCTACTTGCGGAGTTCTCTTTGCGGCATAGCAAGAATGCAATCAGTATCGCCACCGAGTACGCAAAGAGGAAGGGTACAGAGGAATTGGACTCGAATGGCAAGAATTTGGCCTCGAATGGCAAGAATTTGGCCTCTTGA

Protein sequence

FLFYSPHFLLPLSEPSNLCKQRAAMASCRIAARSSLILLESCSPRRTQRILPSVSLPSPPLSSLSPFFTIVTNQSSRNNPNAVPARCFASISAATSSIEVADSAGDLEPPEIHTQDTVEHLLTHSDDVARLMKMERKPIADGAEYPCKGRWFPYLDRFSCGSSWLSSGEILEALDPLILDSRKQRFKNVVRNRSYSVCLVVEGLSDFGNVSAAFRSADALGFQSVHVVSCDASKRCNNDGAALFPLCWRDFVTVHGAFTLLLFNGSLEFNQAIAIAGFICFCIFFCLLPTSLQFQCRYRENRHVSMGAEKWLDIELWNSTPECFKILKSRGYRIVSTHLGTNAVSVYDMDWSCPTAIVVGNESRGISDEALELSDLHCSIPMNGMVDSFNVSVAAGILMHHAVCDRTSRLGYHGDLTLDERQILLAEFSLRHSKNAISIATEYAKRKGTEELDSNGKNLASNGKNLAS

Homology

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BLAST of Spg015728 vs. NCBI nr
Match: XP_038905162.1 (uncharacterized protein LOC120091275 isoform X2 [Benincasa hispida])

HSP 1 Score: 634.0 bits (1634), Expect = 1.0e-177
Identity = 330/426 (77.46%), Postives = 345/426 (80.99%), Query Frame = 0


Query: 25  MASCRIAARSSLILLESCSPRRTQRILPSVSLPSPPLSSLSPFFTIVTNQSSRNNPNAVP 84
           MASCRIAARSSLILLESCS RR Q I PSVSLPS PLSS SPFFTIVTN+ SR+NPNAV 
Sbjct: 1   MASCRIAARSSLILLESCSSRRPQSIFPSVSLPSLPLSSFSPFFTIVTNRLSRSNPNAVS 60

Query: 85  ARCFASISAATSSIEVADSAGDLEPPEIHTQDTVEHLLTHSDDVARLMKMERKPI-ADGA 144
            RCFAS SAATSSIEVAD+ GD EPPEIHTQDTVEHLLTHSDDVARLMKMER+P   DGA
Sbjct: 61  TRCFASFSAATSSIEVADAVGDREPPEIHTQDTVEHLLTHSDDVARLMKMERRPFELDGA 120

Query: 145 EYPCKGRWFPYLDRFSCGSSWLSSGEILEALDPLILDSRKQRFKNVVRNRSYSVCLVVEG 204
           EYP +GRWFPYLD+FSCGSSWL+SGEILEALDPLILDSRK+RFKNVVRNRSYSVCLVVEG
Sbjct: 121 EYPHRGRWFPYLDKFSCGSSWLNSGEILEALDPLILDSRKERFKNVVRNRSYSVCLVVEG 180

Query: 205 LSDFGNVSAAFRSADALGFQSVHVVSCDASKRCNNDGAALFPLCWRDFVTVHGAFTLLLF 264
           LSDFGNVSAAFRSADALGFQSVHVVSCDASK                             
Sbjct: 181 LSDFGNVSAAFRSADALGFQSVHVVSCDASK----------------------------- 240

Query: 265 NGSLEFNQAIAIAGFICFCIFFCLLPTSLQFQCRYRENRHVSMGAEKWLDIELWNSTPEC 324
                                            RYRENRHVSMGAEKWLDIELWNSTPEC
Sbjct: 241 ---------------------------------RYRENRHVSMGAEKWLDIELWNSTPEC 300

Query: 325 FKILKSRGYRIVSTHLGTNAVSVYDMDWSCPTAIVVGNESRGISDEALELSDLHCSIPMN 384
           F++LKSRGYRIVSTHLGTNAVSVYDMDWSCPTAIVVGNESRGISDEALELSDLHCSIPMN
Sbjct: 301 FRVLKSRGYRIVSTHLGTNAVSVYDMDWSCPTAIVVGNESRGISDEALELSDLHCSIPMN 360

Query: 385 GMVDSFNVSVAAGILMHHAVCDRTSRLGYHGDLTLDERQILLAEFSLRHSKNAISIATEY 444
           GMVDSFNVSVAAGILMHHAVCDRTSRLG HGDLT +ERQILLAEFSLRHSK+AIS+A E+
Sbjct: 361 GMVDSFNVSVAAGILMHHAVCDRTSRLGCHGDLTSEERQILLAEFSLRHSKSAISVANEF 364

Query: 445 AKRKGT 450
           AKRKG+
Sbjct: 421 AKRKGS 364

[Close]

BLAST of Spg015728 vs. NCBI nr
Match: XP_038905163.1 (uncharacterized protein LOC120091275 isoform X3 [Benincasa hispida])

HSP 1 Score: 614.4 bits (1583), Expect = 8.2e-172
Identity = 324/426 (76.06%), Postives = 338/426 (79.34%), Query Frame = 0


Query: 25  MASCRIAARSSLILLESCSPRRTQRILPSVSLPSPPLSSLSPFFTIVTNQSSRNNPNAVP 84
           MASCRIAARSSLILLESCS RR Q I PSVSLPS PLSS SPFFTIVTN+ S        
Sbjct: 1   MASCRIAARSSLILLESCSSRRPQSIFPSVSLPSLPLSSFSPFFTIVTNRLS-------- 60

Query: 85  ARCFASISAATSSIEVADSAGDLEPPEIHTQDTVEHLLTHSDDVARLMKMERKPI-ADGA 144
            RCFAS SAATSSIEVAD+ GD EPPEIHTQDTVEHLLTHSDDVARLMKMER+P   DGA
Sbjct: 61  TRCFASFSAATSSIEVADAVGDREPPEIHTQDTVEHLLTHSDDVARLMKMERRPFELDGA 120

Query: 145 EYPCKGRWFPYLDRFSCGSSWLSSGEILEALDPLILDSRKQRFKNVVRNRSYSVCLVVEG 204
           EYP +GRWFPYLD+FSCGSSWL+SGEILEALDPLILDSRK+RFKNVVRNRSYSVCLVVEG
Sbjct: 121 EYPHRGRWFPYLDKFSCGSSWLNSGEILEALDPLILDSRKERFKNVVRNRSYSVCLVVEG 180

Query: 205 LSDFGNVSAAFRSADALGFQSVHVVSCDASKRCNNDGAALFPLCWRDFVTVHGAFTLLLF 264
           LSDFGNVSAAFRSADALGFQSVHVVSCDASK                             
Sbjct: 181 LSDFGNVSAAFRSADALGFQSVHVVSCDASK----------------------------- 240

Query: 265 NGSLEFNQAIAIAGFICFCIFFCLLPTSLQFQCRYRENRHVSMGAEKWLDIELWNSTPEC 324
                                            RYRENRHVSMGAEKWLDIELWNSTPEC
Sbjct: 241 ---------------------------------RYRENRHVSMGAEKWLDIELWNSTPEC 300

Query: 325 FKILKSRGYRIVSTHLGTNAVSVYDMDWSCPTAIVVGNESRGISDEALELSDLHCSIPMN 384
           F++LKSRGYRIVSTHLGTNAVSVYDMDWSCPTAIVVGNESRGISDEALELSDLHCSIPMN
Sbjct: 301 FRVLKSRGYRIVSTHLGTNAVSVYDMDWSCPTAIVVGNESRGISDEALELSDLHCSIPMN 356

Query: 385 GMVDSFNVSVAAGILMHHAVCDRTSRLGYHGDLTLDERQILLAEFSLRHSKNAISIATEY 444
           GMVDSFNVSVAAGILMHHAVCDRTSRLG HGDLT +ERQILLAEFSLRHSK+AIS+A E+
Sbjct: 361 GMVDSFNVSVAAGILMHHAVCDRTSRLGCHGDLTSEERQILLAEFSLRHSKSAISVANEF 356

Query: 445 AKRKGT 450
           AKRKG+
Sbjct: 421 AKRKGS 356

[Close]

BLAST of Spg015728 vs. NCBI nr
Match: XP_022933839.1 (uncharacterized protein LOC111441136 [Cucurbita moschata])

HSP 1 Score: 611.7 bits (1576), Expect = 5.3e-171
Identity = 321/427 (75.18%), Postives = 337/427 (78.92%), Query Frame = 0


Query: 25  MASCRIAARSSLILLESCSPRRTQRILPSVSLPSPPLSSLSPFFTIVTNQSSRNNPNAVP 84
           MASCRIA R SL LL SCSPR TQRILPSVS  SPPLSS SPFFT V+N SSRNNP AV 
Sbjct: 1   MASCRIAVRPSLFLLGSCSPRTTQRILPSVSFSSPPLSSYSPFFTAVSNHSSRNNPKAVS 60

Query: 85  ARCFASISAATSSIEVADSAG-DLEPPEIHTQDTVEHLLTHSDDVARLMKMERKPI-ADG 144
            RCF+S SAA SS+EV DS G DLEPPEIHTQDTVEHLLTHSDDVARLMKMER+PI  DG
Sbjct: 61  IRCFSSTSAAASSVEVTDSVGSDLEPPEIHTQDTVEHLLTHSDDVARLMKMERRPINLDG 120

Query: 145 AEYPCKGRWFPYLDRFSCGSSWLSSGEILEALDPLILDSRKQRFKNVVRNRSYSVCLVVE 204
            EYP +GRWFPYLD+FSC SSWL+SGE+L+ALDPLILDSRK+RFKNVVR+RSYSVCLVVE
Sbjct: 121 DEYPRQGRWFPYLDKFSCESSWLNSGEVLDALDPLILDSRKERFKNVVRHRSYSVCLVVE 180

Query: 205 GLSDFGNVSAAFRSADALGFQSVHVVSCDASKRCNNDGAALFPLCWRDFVTVHGAFTLLL 264
           GLSDFGNVSAAFRSADALGFQSVHVVSCDASK                            
Sbjct: 181 GLSDFGNVSAAFRSADALGFQSVHVVSCDASK---------------------------- 240

Query: 265 FNGSLEFNQAIAIAGFICFCIFFCLLPTSLQFQCRYRENRHVSMGAEKWLDIELWNSTPE 324
                                             RYRENRHVSMGAEKWLDIELWNSTPE
Sbjct: 241 ----------------------------------RYRENRHVSMGAEKWLDIELWNSTPE 300

Query: 325 CFKILKSRGYRIVSTHLGTNAVSVYDMDWSCPTAIVVGNESRGISDEALELSDLHCSIPM 384
           CF+ILKSRGYRIVSTHLG+NAVSVYDMDWSCPTAIVVGNESRGISDEALELSDLHCSIPM
Sbjct: 301 CFRILKSRGYRIVSTHLGSNAVSVYDMDWSCPTAIVVGNESRGISDEALELSDLHCSIPM 360

Query: 385 NGMVDSFNVSVAAGILMHHAVCDRTSRLGYHGDLTLDERQILLAEFSLRHSKNAISIATE 444
           NGMVDSFNVSVAAGILMHHAVCDRTSRLG HGDLT  ERQILLAEFSLRH+KNAIS+A E
Sbjct: 361 NGMVDSFNVSVAAGILMHHAVCDRTSRLGCHGDLTSIERQILLAEFSLRHNKNAISVAHE 365

Query: 445 YAKRKGT 450
           YAKRK +
Sbjct: 421 YAKRKSS 365

[Close]

BLAST of Spg015728 vs. NCBI nr
Match: KAG6588498.1 (hypothetical protein SDJN03_17063, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 608.6 bits (1568), Expect = 4.5e-170
Identity = 320/427 (74.94%), Postives = 336/427 (78.69%), Query Frame = 0


Query: 25  MASCRIAARSSLILLESCSPRRTQRILPSVSLPSPPLSSLSPFFTIVTNQSSRNNPNAVP 84
           MASCRIA R SL LL SCSPR TQRILPSVS  SPPLSS SPFFT V+N SSRNNP AV 
Sbjct: 1   MASCRIAVRPSLFLLGSCSPRTTQRILPSVSFSSPPLSSYSPFFTAVSNHSSRNNPKAVS 60

Query: 85  ARCFASISAATSSIEVADSAG-DLEPPEIHTQDTVEHLLTHSDDVARLMKMERKPI-ADG 144
            RCF+S SAA SS+EV DS G DLEPPEIHTQDTVEHLLTHS DVARLMKMER+PI  DG
Sbjct: 61  TRCFSSTSAAASSVEVTDSVGSDLEPPEIHTQDTVEHLLTHSYDVARLMKMERRPINLDG 120

Query: 145 AEYPCKGRWFPYLDRFSCGSSWLSSGEILEALDPLILDSRKQRFKNVVRNRSYSVCLVVE 204
            EYP +GRWFPYLD+FSC SSWL+SGE+L+ALDPLILDSRK+RFKNVVR+RSYSVCLVVE
Sbjct: 121 DEYPRQGRWFPYLDKFSCESSWLNSGEVLDALDPLILDSRKERFKNVVRHRSYSVCLVVE 180

Query: 205 GLSDFGNVSAAFRSADALGFQSVHVVSCDASKRCNNDGAALFPLCWRDFVTVHGAFTLLL 264
           GLSDFGNVSAAFRSADALGFQSVHVVSCDASK                            
Sbjct: 181 GLSDFGNVSAAFRSADALGFQSVHVVSCDASK---------------------------- 240

Query: 265 FNGSLEFNQAIAIAGFICFCIFFCLLPTSLQFQCRYRENRHVSMGAEKWLDIELWNSTPE 324
                                             RYRENRHVSMGAEKWLDIELWNSTPE
Sbjct: 241 ----------------------------------RYRENRHVSMGAEKWLDIELWNSTPE 300

Query: 325 CFKILKSRGYRIVSTHLGTNAVSVYDMDWSCPTAIVVGNESRGISDEALELSDLHCSIPM 384
           CF+ILKSRGYRIVSTHLG+NAVSVYDMDWSCPTAIVVGNESRGISDEALELSDLHCSIPM
Sbjct: 301 CFRILKSRGYRIVSTHLGSNAVSVYDMDWSCPTAIVVGNESRGISDEALELSDLHCSIPM 360

Query: 385 NGMVDSFNVSVAAGILMHHAVCDRTSRLGYHGDLTLDERQILLAEFSLRHSKNAISIATE 444
           NGMVDSFNVSVAAGILMHHAVCDRTSRLG HGDLT  ERQILLAEFSLRH+KNAIS+A E
Sbjct: 361 NGMVDSFNVSVAAGILMHHAVCDRTSRLGCHGDLTSIERQILLAEFSLRHNKNAISVAHE 365

Query: 445 YAKRKGT 450
           YAKRK +
Sbjct: 421 YAKRKSS 365

[Close]

BLAST of Spg015728 vs. NCBI nr
Match: XP_022969711.1 (uncharacterized protein LOC111468788 [Cucurbita maxima])

HSP 1 Score: 607.1 bits (1564), Expect = 1.3e-169
Identity = 318/427 (74.47%), Postives = 337/427 (78.92%), Query Frame = 0


Query: 25  MASCRIAARSSLILLESCSPRRTQRILPSVSLPSPPLSSLSPFFTIVTNQSSRNNPNAVP 84
           MASCRIA R SL LL SCS R TQ ILPSVS PSPPLSS SPFFT V+N SSRNNP AV 
Sbjct: 1   MASCRIAVRPSLFLLGSCSSRTTQLILPSVSFPSPPLSSYSPFFTAVSNHSSRNNPKAVF 60

Query: 85  ARCFASISAATSSIEVADSAG-DLEPPEIHTQDTVEHLLTHSDDVARLMKMERKPI-ADG 144
            RCF+S SAA SS+EV D+ G DLEPPEIHTQDTVEHLLTHSDDVARLMKMER+PI  DG
Sbjct: 61  TRCFSSTSAAASSVEVTDAVGSDLEPPEIHTQDTVEHLLTHSDDVARLMKMERRPINLDG 120

Query: 145 AEYPCKGRWFPYLDRFSCGSSWLSSGEILEALDPLILDSRKQRFKNVVRNRSYSVCLVVE 204
           AEYP +GRWFPYLD+FSC +SWL+SGE+L+ALDPLILDSRK+RFKNVVR+RSYSVCLVVE
Sbjct: 121 AEYPHQGRWFPYLDKFSCENSWLNSGEVLDALDPLILDSRKERFKNVVRHRSYSVCLVVE 180

Query: 205 GLSDFGNVSAAFRSADALGFQSVHVVSCDASKRCNNDGAALFPLCWRDFVTVHGAFTLLL 264
           GLSDFGNVSAAFRSADALGFQSVHVVSCDASK                            
Sbjct: 181 GLSDFGNVSAAFRSADALGFQSVHVVSCDASK---------------------------- 240

Query: 265 FNGSLEFNQAIAIAGFICFCIFFCLLPTSLQFQCRYRENRHVSMGAEKWLDIELWNSTPE 324
                                             RYRENRHVSMGAEKWLDIELWNSTPE
Sbjct: 241 ----------------------------------RYRENRHVSMGAEKWLDIELWNSTPE 300

Query: 325 CFKILKSRGYRIVSTHLGTNAVSVYDMDWSCPTAIVVGNESRGISDEALELSDLHCSIPM 384
           CF+ILKSRGYRIVSTHLG+NAVSVYDMDWSCPTAIVVGNESRGISDEALELSDLHC+IPM
Sbjct: 301 CFRILKSRGYRIVSTHLGSNAVSVYDMDWSCPTAIVVGNESRGISDEALELSDLHCTIPM 360

Query: 385 NGMVDSFNVSVAAGILMHHAVCDRTSRLGYHGDLTLDERQILLAEFSLRHSKNAISIATE 444
           NGMVDSFNVSVAAGILMHHAVCDRTSRLG HGDLT  ERQILLAEFSLRH+KNAIS+A E
Sbjct: 361 NGMVDSFNVSVAAGILMHHAVCDRTSRLGCHGDLTSIERQILLAEFSLRHNKNAISVAHE 365

Query: 445 YAKRKGT 450
           YAKRK +
Sbjct: 421 YAKRKSS 365

[Close]

BLAST of Spg015728 vs. ExPASy Swiss-Prot
Match: O67577 (tRNA (guanosine(18)-2'-O)-methyltransferase OS=Aquifex aeolicus (strain VF5) OX=224324 GN=trmH PE=1 SV=1)

HSP 1 Score: 92.0 bits (227), Expect = 1.9e-17
Identity = 46/123 (37.40%), Postives = 76/123 (61.79%), Query Frame = 0


Query: 301 NRHVSMGAEKWLDIELWNSTPECFKILKSRGYRIVSTHLGTNAVSVYDMDWSCPTAIVVG 360
           N  ++ G+ KW+ IE  ++  +     K+RG++IV+T L   +V+  ++D++ PT +VVG
Sbjct: 67  NEGITQGSHKWVFIEKVDNPVQKLLEFKNRGFQIVATWLSKESVNFREVDYTKPTVLVVG 126

Query: 361 NESRGISDEALELSDLHCSIPMNGMVDSFNVSVAAGILMHHAVCDRTSRLGYHGDLTLDE 420
           NE +G+S E +E++D    IPM GM  S NVSVA GI+++ A   R  + G +   +L E
Sbjct: 127 NELQGVSPEIVEIADKKIVIPMYGMAQSLNVSVATGIILYEAQRQREEK-GMYSRPSLSE 186

Query: 421 RQI 424
            +I
Sbjct: 187 EEI 188

[Close]

BLAST of Spg015728 vs. ExPASy Swiss-Prot
Match: P0AGJ3 (tRNA (guanosine(18)-2'-O)-methyltransferase OS=Escherichia coli O157:H7 OX=83334 GN=trmH PE=3 SV=1)

HSP 1 Score: 89.0 bits (219), Expect = 1.6e-16
Identity = 53/155 (34.19%), Postives = 82/155 (52.90%), Query Frame = 0


Query: 297 RYRENRHVSMGAEKWLDIELWNSTPECFKILKSRGYRIVSTHLGTNAVSVYDMDWSCPTA 356
           R R     + G+  W+ ++   +  +    LK +G +I++THL  NAV   ++D++ PT 
Sbjct: 56  RMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNAVDFREIDYTRPTC 115

Query: 357 IVVGNESRGISDEALELSDLHCSIPMNGMVDSFNVSVAAGILMHHAVCDRTSR---LGYH 416
           I++G E  GI+ EAL L+D    IPM GMV S NVSVA+ ++++ A   R +    L  +
Sbjct: 116 ILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYEAQRQRQNAGMYLREN 175

Query: 417 GDLTLDERQILLAEFSLRHSKNAISIATEYAKRKG 449
             L   E+Q LL E           +  + AKRKG
Sbjct: 176 SMLPEAEQQRLLFE-------GGYPVLAKVAKRKG 203

[Close]

BLAST of Spg015728 vs. ExPASy Swiss-Prot
Match: P0AGJ2 (tRNA (guanosine(18)-2'-O)-methyltransferase OS=Escherichia coli (strain K12) OX=83333 GN=trmH PE=1 SV=1)

HSP 1 Score: 89.0 bits (219), Expect = 1.6e-16
Identity = 53/155 (34.19%), Postives = 82/155 (52.90%), Query Frame = 0


Query: 297 RYRENRHVSMGAEKWLDIELWNSTPECFKILKSRGYRIVSTHLGTNAVSVYDMDWSCPTA 356
           R R     + G+  W+ ++   +  +    LK +G +I++THL  NAV   ++D++ PT 
Sbjct: 56  RMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNAVDFREIDYTRPTC 115

Query: 357 IVVGNESRGISDEALELSDLHCSIPMNGMVDSFNVSVAAGILMHHAVCDRTSR---LGYH 416
           I++G E  GI+ EAL L+D    IPM GMV S NVSVA+ ++++ A   R +    L  +
Sbjct: 116 ILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYEAQRQRQNAGMYLREN 175

Query: 417 GDLTLDERQILLAEFSLRHSKNAISIATEYAKRKG 449
             L   E+Q LL E           +  + AKRKG
Sbjct: 176 SMLPEAEQQRLLFE-------GGYPVLAKVAKRKG 203

[Close]

BLAST of Spg015728 vs. ExPASy Swiss-Prot
Match: P0AGJ4 (tRNA (guanosine(18)-2'-O)-methyltransferase OS=Shigella flexneri OX=623 GN=trmH PE=3 SV=1)

HSP 1 Score: 89.0 bits (219), Expect = 1.6e-16
Identity = 53/155 (34.19%), Postives = 82/155 (52.90%), Query Frame = 0


Query: 297 RYRENRHVSMGAEKWLDIELWNSTPECFKILKSRGYRIVSTHLGTNAVSVYDMDWSCPTA 356
           R R     + G+  W+ ++   +  +    LK +G +I++THL  NAV   ++D++ PT 
Sbjct: 56  RMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNAVDFREIDYTRPTC 115

Query: 357 IVVGNESRGISDEALELSDLHCSIPMNGMVDSFNVSVAAGILMHHAVCDRTSR---LGYH 416
           I++G E  GI+ EAL L+D    IPM GMV S NVSVA+ ++++ A   R +    L  +
Sbjct: 116 ILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYEAQRQRQNAGMYLREN 175

Query: 417 GDLTLDERQILLAEFSLRHSKNAISIATEYAKRKG 449
             L   E+Q LL E           +  + AKRKG
Sbjct: 176 SMLPEAEQQRLLFE-------GGYPVLAKVAKRKG 203

[Close]

BLAST of Spg015728 vs. ExPASy Swiss-Prot
Match: Q72GI1 (tRNA (guanosine(18)-2'-O)-methyltransferase OS=Thermus thermophilus (strain ATCC BAA-163 / DSM 7039 / HB27) OX=262724 GN=trmH PE=1 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 1.9e-14
Identity = 40/98 (40.82%), Postives = 61/98 (62.24%), Query Frame = 0


Query: 305 SMGAEKWLDIELWNSTPECFKILKSRGYRIVSTHLGTNAVSVYDMDWSCPTAIVVGNESR 364
           S G+ KW+ + +     E F+ LK RG+ + +T L  +A    ++D++ PTA++ G E  
Sbjct: 67  SGGSHKWVYLRVHPDLHEAFRFLKERGFTVYATALREDARDFREVDYTKPTAVLFGAEKW 126

Query: 365 GISDEALELSDLHCSIPMNGMVDSFNVSVAAGILMHHA 403
           G+S+EAL L+D    IPM GMV S NVSVAA +++  A
Sbjct: 127 GVSEEALALADGAIKIPMLGMVQSLNVSVAAAVILFEA 164

[Close]

BLAST of Spg015728 vs. ExPASy TrEMBL
Match: A0A6J1F0V5 (uncharacterized protein LOC111441136 OS=Cucurbita moschata OX=3662 GN=LOC111441136 PE=3 SV=1)

HSP 1 Score: 611.7 bits (1576), Expect = 2.6e-171
Identity = 321/427 (75.18%), Postives = 337/427 (78.92%), Query Frame = 0


Query: 25  MASCRIAARSSLILLESCSPRRTQRILPSVSLPSPPLSSLSPFFTIVTNQSSRNNPNAVP 84
           MASCRIA R SL LL SCSPR TQRILPSVS  SPPLSS SPFFT V+N SSRNNP AV 
Sbjct: 1   MASCRIAVRPSLFLLGSCSPRTTQRILPSVSFSSPPLSSYSPFFTAVSNHSSRNNPKAVS 60

Query: 85  ARCFASISAATSSIEVADSAG-DLEPPEIHTQDTVEHLLTHSDDVARLMKMERKPI-ADG 144
            RCF+S SAA SS+EV DS G DLEPPEIHTQDTVEHLLTHSDDVARLMKMER+PI  DG
Sbjct: 61  IRCFSSTSAAASSVEVTDSVGSDLEPPEIHTQDTVEHLLTHSDDVARLMKMERRPINLDG 120

Query: 145 AEYPCKGRWFPYLDRFSCGSSWLSSGEILEALDPLILDSRKQRFKNVVRNRSYSVCLVVE 204
            EYP +GRWFPYLD+FSC SSWL+SGE+L+ALDPLILDSRK+RFKNVVR+RSYSVCLVVE
Sbjct: 121 DEYPRQGRWFPYLDKFSCESSWLNSGEVLDALDPLILDSRKERFKNVVRHRSYSVCLVVE 180

Query: 205 GLSDFGNVSAAFRSADALGFQSVHVVSCDASKRCNNDGAALFPLCWRDFVTVHGAFTLLL 264
           GLSDFGNVSAAFRSADALGFQSVHVVSCDASK                            
Sbjct: 181 GLSDFGNVSAAFRSADALGFQSVHVVSCDASK---------------------------- 240

Query: 265 FNGSLEFNQAIAIAGFICFCIFFCLLPTSLQFQCRYRENRHVSMGAEKWLDIELWNSTPE 324
                                             RYRENRHVSMGAEKWLDIELWNSTPE
Sbjct: 241 ----------------------------------RYRENRHVSMGAEKWLDIELWNSTPE 300

Query: 325 CFKILKSRGYRIVSTHLGTNAVSVYDMDWSCPTAIVVGNESRGISDEALELSDLHCSIPM 384
           CF+ILKSRGYRIVSTHLG+NAVSVYDMDWSCPTAIVVGNESRGISDEALELSDLHCSIPM
Sbjct: 301 CFRILKSRGYRIVSTHLGSNAVSVYDMDWSCPTAIVVGNESRGISDEALELSDLHCSIPM 360

Query: 385 NGMVDSFNVSVAAGILMHHAVCDRTSRLGYHGDLTLDERQILLAEFSLRHSKNAISIATE 444
           NGMVDSFNVSVAAGILMHHAVCDRTSRLG HGDLT  ERQILLAEFSLRH+KNAIS+A E
Sbjct: 361 NGMVDSFNVSVAAGILMHHAVCDRTSRLGCHGDLTSIERQILLAEFSLRHNKNAISVAHE 365

Query: 445 YAKRKGT 450
           YAKRK +
Sbjct: 421 YAKRKSS 365

[Close]

BLAST of Spg015728 vs. ExPASy TrEMBL
Match: A0A6J1HYK4 (uncharacterized protein LOC111468788 OS=Cucurbita maxima OX=3661 GN=LOC111468788 PE=3 SV=1)

HSP 1 Score: 607.1 bits (1564), Expect = 6.4e-170
Identity = 318/427 (74.47%), Postives = 337/427 (78.92%), Query Frame = 0


Query: 25  MASCRIAARSSLILLESCSPRRTQRILPSVSLPSPPLSSLSPFFTIVTNQSSRNNPNAVP 84
           MASCRIA R SL LL SCS R TQ ILPSVS PSPPLSS SPFFT V+N SSRNNP AV 
Sbjct: 1   MASCRIAVRPSLFLLGSCSSRTTQLILPSVSFPSPPLSSYSPFFTAVSNHSSRNNPKAVF 60

Query: 85  ARCFASISAATSSIEVADSAG-DLEPPEIHTQDTVEHLLTHSDDVARLMKMERKPI-ADG 144
            RCF+S SAA SS+EV D+ G DLEPPEIHTQDTVEHLLTHSDDVARLMKMER+PI  DG
Sbjct: 61  TRCFSSTSAAASSVEVTDAVGSDLEPPEIHTQDTVEHLLTHSDDVARLMKMERRPINLDG 120

Query: 145 AEYPCKGRWFPYLDRFSCGSSWLSSGEILEALDPLILDSRKQRFKNVVRNRSYSVCLVVE 204
           AEYP +GRWFPYLD+FSC +SWL+SGE+L+ALDPLILDSRK+RFKNVVR+RSYSVCLVVE
Sbjct: 121 AEYPHQGRWFPYLDKFSCENSWLNSGEVLDALDPLILDSRKERFKNVVRHRSYSVCLVVE 180

Query: 205 GLSDFGNVSAAFRSADALGFQSVHVVSCDASKRCNNDGAALFPLCWRDFVTVHGAFTLLL 264
           GLSDFGNVSAAFRSADALGFQSVHVVSCDASK                            
Sbjct: 181 GLSDFGNVSAAFRSADALGFQSVHVVSCDASK---------------------------- 240

Query: 265 FNGSLEFNQAIAIAGFICFCIFFCLLPTSLQFQCRYRENRHVSMGAEKWLDIELWNSTPE 324
                                             RYRENRHVSMGAEKWLDIELWNSTPE
Sbjct: 241 ----------------------------------RYRENRHVSMGAEKWLDIELWNSTPE 300

Query: 325 CFKILKSRGYRIVSTHLGTNAVSVYDMDWSCPTAIVVGNESRGISDEALELSDLHCSIPM 384
           CF+ILKSRGYRIVSTHLG+NAVSVYDMDWSCPTAIVVGNESRGISDEALELSDLHC+IPM
Sbjct: 301 CFRILKSRGYRIVSTHLGSNAVSVYDMDWSCPTAIVVGNESRGISDEALELSDLHCTIPM 360

Query: 385 NGMVDSFNVSVAAGILMHHAVCDRTSRLGYHGDLTLDERQILLAEFSLRHSKNAISIATE 444
           NGMVDSFNVSVAAGILMHHAVCDRTSRLG HGDLT  ERQILLAEFSLRH+KNAIS+A E
Sbjct: 361 NGMVDSFNVSVAAGILMHHAVCDRTSRLGCHGDLTSIERQILLAEFSLRHNKNAISVAHE 365

Query: 445 YAKRKGT 450
           YAKRK +
Sbjct: 421 YAKRKSS 365

[Close]

BLAST of Spg015728 vs. ExPASy TrEMBL
Match: A0A0A0LC15 (SpoU_methylase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G824750 PE=3 SV=1)

HSP 1 Score: 589.0 bits (1517), Expect = 1.8e-164
Identity = 312/426 (73.24%), Postives = 332/426 (77.93%), Query Frame = 0


Query: 25  MASCRIAARSSLILLESCSPRRTQRILPSVSLPSPPLSSLSPFFTIVTNQSSRNNPNAVP 84
           MASCRI +RSSL LL+SCSPRRTQRI  SV LPS  L S S FF   TN+ SR+NPNAV 
Sbjct: 1   MASCRIVSRSSLFLLQSCSPRRTQRIFSSVFLPSLHLPSFSLFF---TNRFSRSNPNAVS 60

Query: 85  ARCFASISAATSSIEVADSAGDLEPPEIHTQDTVEHLLTHSDDVARLMKMERKPI-ADGA 144
            RCFASISAATS +EV D   D+EPPE+ TQDTVEHLLTHS+DVARLMKMER+ +  DGA
Sbjct: 61  TRCFASISAATSPLEVEDIVADVEPPEVRTQDTVEHLLTHSNDVARLMKMERRSVELDGA 120

Query: 145 EYPCKGRWFPYLDRFSCGSSWLSSGEILEALDPLILDSRKQRFKNVVRNRSYSVCLVVEG 204
           EYP KGRWFPYLD++SCGSSWL+SGEILEALD LILDSRK+RFKNVVRNRSYSVCLVVEG
Sbjct: 121 EYPHKGRWFPYLDKYSCGSSWLNSGEILEALDSLILDSRKERFKNVVRNRSYSVCLVVEG 180

Query: 205 LSDFGNVSAAFRSADALGFQSVHVVSCDASKRCNNDGAALFPLCWRDFVTVHGAFTLLLF 264
           L+DFGNVSAAFRSADALGFQSVHVVSCDASK                             
Sbjct: 181 LTDFGNVSAAFRSADALGFQSVHVVSCDASK----------------------------- 240

Query: 265 NGSLEFNQAIAIAGFICFCIFFCLLPTSLQFQCRYRENRHVSMGAEKWLDIELWNSTPEC 324
                                            RYRENRHVSMGAEKWLDIELWNSTPEC
Sbjct: 241 ---------------------------------RYRENRHVSMGAEKWLDIELWNSTPEC 300

Query: 325 FKILKSRGYRIVSTHLGTNAVSVYDMDWSCPTAIVVGNESRGISDEALELSDLHCSIPMN 384
           FKILKSRGYRIVSTHLG NAVSVYDMDWSCPTAIVVGNESRGIS+EALELSDLHCSIPMN
Sbjct: 301 FKILKSRGYRIVSTHLGANAVSVYDMDWSCPTAIVVGNESRGISNEALELSDLHCSIPMN 360

Query: 385 GMVDSFNVSVAAGILMHHAVCDRTSRLGYHGDLTLDERQILLAEFSLRHSKNAISIATEY 444
           GMVDSFNVSVAAGILMHHAVC+RTSRLG HGDLT +ERQILLAEFSLRHSK+AISIA E 
Sbjct: 361 GMVDSFNVSVAAGILMHHAVCNRTSRLGCHGDLTSEERQILLAEFSLRHSKSAISIANEL 361

Query: 445 AKRKGT 450
           AKRKG+
Sbjct: 421 AKRKGS 361

[Close]

BLAST of Spg015728 vs. ExPASy TrEMBL
Match: A0A5A7UJ80 (tRNA (Guanosine(18)-2'-O)-methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G007650 PE=3 SV=1)

HSP 1 Score: 584.3 bits (1505), Expect = 4.4e-163
Identity = 312/426 (73.24%), Postives = 333/426 (78.17%), Query Frame = 0


Query: 25  MASCRIAARSSLILLESCSPRRTQRILPSVSLPSPPLSSLSPFFTIVTNQSSRNNPNAVP 84
           MASCRI +RSSL LL+S SPRRTQR+ PSV LPS  L S SPFF   TN  SR+NPNAV 
Sbjct: 1   MASCRIVSRSSLTLLQSFSPRRTQRVFPSVFLPSLHLPS-SPFF---TNTLSRSNPNAVS 60

Query: 85  ARCFASISAATSSIEVADSAGDLEPPEIHTQDTVEHLLTHSDDVARLMKMERKPI-ADGA 144
           +RCFASISAATS +E+AD   D+EPPE+ TQDTVEHLLTHSDDV+RLMKMER+P+  DGA
Sbjct: 61  SRCFASISAATSPLELADIVADVEPPEVPTQDTVEHLLTHSDDVSRLMKMERRPVELDGA 120

Query: 145 EYPCKGRWFPYLDRFSCGSSWLSSGEILEALDPLILDSRKQRFKNVVRNRSYSVCLVVEG 204
           EYP KGRWFPYLD++S GSSWL+SGEILEALD LILD+RK+RFKNVVRNRSYSVCLVVEG
Sbjct: 121 EYPHKGRWFPYLDKYSFGSSWLNSGEILEALDSLILDTRKERFKNVVRNRSYSVCLVVEG 180

Query: 205 LSDFGNVSAAFRSADALGFQSVHVVSCDASKRCNNDGAALFPLCWRDFVTVHGAFTLLLF 264
           LSDFGNVSAAFRSADALGFQSVHVVSCDASK                             
Sbjct: 181 LSDFGNVSAAFRSADALGFQSVHVVSCDASK----------------------------- 240

Query: 265 NGSLEFNQAIAIAGFICFCIFFCLLPTSLQFQCRYRENRHVSMGAEKWLDIELWNSTPEC 324
                                            RYRENRHVSMGAEKWLDIELWNST EC
Sbjct: 241 ---------------------------------RYRENRHVSMGAEKWLDIELWNSTSEC 300

Query: 325 FKILKSRGYRIVSTHLGTNAVSVYDMDWSCPTAIVVGNESRGISDEALELSDLHCSIPMN 384
           FKILKSRGYRIVSTHLG NAVSVYDMDWSCPTAIVVGNESRGIS+EALELSDLHCSIPMN
Sbjct: 301 FKILKSRGYRIVSTHLGANAVSVYDMDWSCPTAIVVGNESRGISNEALELSDLHCSIPMN 360

Query: 385 GMVDSFNVSVAAGILMHHAVCDRTSRLGYHGDLTLDERQILLAEFSLRHSKNAISIATEY 444
           GMVDSFNVSVAAGILMHHAVCDRTSRLG HGDLT +ERQILLAEFSLRHSK+AISIA E 
Sbjct: 361 GMVDSFNVSVAAGILMHHAVCDRTSRLGCHGDLTSEERQILLAEFSLRHSKSAISIANEL 360

Query: 445 AKRKGT 450
           AKRKG+
Sbjct: 421 AKRKGS 360

[Close]

BLAST of Spg015728 vs. ExPASy TrEMBL
Match: A0A1S3B8M3 (tRNA (Guanosine(18)-2'-O)-methyltransferase OS=Cucumis melo OX=3656 GN=LOC103486969 PE=3 SV=1)

HSP 1 Score: 584.3 bits (1505), Expect = 4.4e-163
Identity = 312/426 (73.24%), Postives = 333/426 (78.17%), Query Frame = 0


Query: 25  MASCRIAARSSLILLESCSPRRTQRILPSVSLPSPPLSSLSPFFTIVTNQSSRNNPNAVP 84
           MASCRI +RSSL LL+S SPRRTQR+ PSV LPS  L S SPFF   TN  SR+NPNAV 
Sbjct: 1   MASCRIVSRSSLTLLQSFSPRRTQRVFPSVFLPSLHLPS-SPFF---TNTLSRSNPNAVS 60

Query: 85  ARCFASISAATSSIEVADSAGDLEPPEIHTQDTVEHLLTHSDDVARLMKMERKPI-ADGA 144
           +RCFASISAATS +E+AD   D+EPPE+ TQDTVEHLLTHSDDV+RLMKMER+P+  DGA
Sbjct: 61  SRCFASISAATSPLELADIVADVEPPEVPTQDTVEHLLTHSDDVSRLMKMERRPVELDGA 120

Query: 145 EYPCKGRWFPYLDRFSCGSSWLSSGEILEALDPLILDSRKQRFKNVVRNRSYSVCLVVEG 204
           EYP KGRWFPYLD++S GSSWL+SGEILEALD LILD+RK+RFKNVVRNRSYSVCLVVEG
Sbjct: 121 EYPHKGRWFPYLDKYSFGSSWLNSGEILEALDSLILDTRKERFKNVVRNRSYSVCLVVEG 180

Query: 205 LSDFGNVSAAFRSADALGFQSVHVVSCDASKRCNNDGAALFPLCWRDFVTVHGAFTLLLF 264
           LSDFGNVSAAFRSADALGFQSVHVVSCDASK                             
Sbjct: 181 LSDFGNVSAAFRSADALGFQSVHVVSCDASK----------------------------- 240

Query: 265 NGSLEFNQAIAIAGFICFCIFFCLLPTSLQFQCRYRENRHVSMGAEKWLDIELWNSTPEC 324
                                            RYRENRHVSMGAEKWLDIELWNST EC
Sbjct: 241 ---------------------------------RYRENRHVSMGAEKWLDIELWNSTSEC 300

Query: 325 FKILKSRGYRIVSTHLGTNAVSVYDMDWSCPTAIVVGNESRGISDEALELSDLHCSIPMN 384
           FKILKSRGYRIVSTHLG NAVSVYDMDWSCPTAIVVGNESRGIS+EALELSDLHCSIPMN
Sbjct: 301 FKILKSRGYRIVSTHLGANAVSVYDMDWSCPTAIVVGNESRGISNEALELSDLHCSIPMN 360

Query: 385 GMVDSFNVSVAAGILMHHAVCDRTSRLGYHGDLTLDERQILLAEFSLRHSKNAISIATEY 444
           GMVDSFNVSVAAGILMHHAVCDRTSRLG HGDLT +ERQILLAEFSLRHSK+AISIA E 
Sbjct: 361 GMVDSFNVSVAAGILMHHAVCDRTSRLGCHGDLTSEERQILLAEFSLRHSKSAISIANEL 360

Query: 445 AKRKGT 450
           AKRKG+
Sbjct: 421 AKRKGS 360

[Close]

BLAST of Spg015728 vs. TAIR 10
Match: AT5G15390.1 (tRNA/rRNA methyltransferase (SpoU) family protein )

HSP 1 Score: 375.9 bits (964), Expect = 4.6e-104
Identity = 210/394 (53.30%), Postives = 247/394 (62.69%), Query Frame = 0


Query: 55  SLPSPPLSSLSPFFTIVTNQSSRNNPNAVPARCFASISAATSSIEVADSAGDLEPPEIHT 114
           S P  PL   +P F+I        +P +V     + I  A  +   A +       E   
Sbjct: 14  SFPFVPLP--NPNFSITFISVRAFSPLSVLHPNSSCIVTARRTFHGAVALSPESLTEESP 73

Query: 115 QDTVEHLL-THSDDVARLMKMERKPIADGAEYPCKGRWFPYLDRFSCGSSWLSSGEILEA 174
           +DTV+ LL T+  + + LMKMER+      E  C+G WFPY DRF CG   LSS E+LEA
Sbjct: 74  KDTVKGLLTTNRGEASSLMKMERRCSLSNGEGDCRGSWFPYEDRFRCGEVHLSSREVLEA 133

Query: 175 LDPLILDSRKQRFKNVVRNRSYSVCLVVEGLSDFGNVSAAFRSADALGFQSVHVVSCDAS 234
           + P +++ R  RF+ VV NRSYSVCLVVEGLSDFGN+SAAFRSADALG QSVHVVSCD+S
Sbjct: 134 VSPHMMEERTDRFRRVVENRSYSVCLVVEGLSDFGNISAAFRSADALGIQSVHVVSCDSS 193

Query: 235 KRCNNDGAALFPLCWRDFVTVHGAFTLLLFNGSLEFNQAIAIAGFICFCIFFCLLPTSLQ 294
           K                                                           
Sbjct: 194 K----------------------------------------------------------- 253

Query: 295 FQCRYRENRHVSMGAEKWLDIELWNSTPECFKILKSRGYRIVSTHLGTNAVSVYDMDWSC 354
              RY  NRHVSMGAEKWLDIE W++  ECFK+LKSRGYRI +THLG + VS+YDMDWSC
Sbjct: 254 ---RYNGNRHVSMGAEKWLDIEFWDTPKECFKVLKSRGYRIATTHLGMDTVSIYDMDWSC 313

Query: 355 PTAIVVGNESRGISDEALELSDLHCSIPMNGMVDSFNVSVAAGILMHHAVCDRTSRLGYH 414
           PTAIVVGNE RGISDEALELSDL CSIPMNGMVDSFNVSVAAGILMHHAV DRT+RLG H
Sbjct: 314 PTAIVVGNEGRGISDEALELSDLRCSIPMNGMVDSFNVSVAAGILMHHAVSDRTTRLGSH 343

Query: 415 GDLTLDERQILLAEFSLRHSKNAISIATEYAKRK 448
           GDL+  E++IL+AEFSLRHS+++I IA E+AKRK
Sbjct: 374 GDLSEAEKEILMAEFSLRHSRSSICIAYEFAKRK 343

[Close]

BLAST of Spg015728 vs. TAIR 10
Match: AT2G19870.1 (tRNA/rRNA methyltransferase (SpoU) family protein )

HSP 1 Score: 43.1 bits (100), Expect = 7.1e-04
Identity = 28/125 (22.40%), Postives = 56/125 (44.80%), Query Frame = 0


Query: 305 SMGAEKWLDIELWNSTPECFKILKSRGYRIVSTHLGTNAVSVYDMDWSCPTAIVVGNESR 364
           S G+ + +++    +  +  +     G+R+V   +   AV++ ++    PT +V+GNE  
Sbjct: 464 SAGSLEVMELRYCKNMMQFLEASAENGWRVVGGSVSPKAVALNEVLPGSPTILVLGNEGT 523

Query: 365 G----ISDEALELSDLHCSIPMNG-----------------MVDSFNVSVAAGILMHHAV 409
           G    +     +L  +  ++P                     V+S NVSVAAG+ +HH +
Sbjct: 524 GLRPLVERSCTDLVRISGNMPNEVAVTESDDAEGEGFRSFLAVESLNVSVAAGLFLHHLI 583

The following BLAST results are available for this feature:

BLAST of Spg015728 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038905162.1	1.0e-177	77.46	uncharacterized protein LOC120091275 isoform X2 [Benincasa hispida]	[more]
XP_038905163.1	8.2e-172	76.06	uncharacterized protein LOC120091275 isoform X3 [Benincasa hispida]	[more]
XP_022933839.1	5.3e-171	75.18	uncharacterized protein LOC111441136 [Cucurbita moschata]	[more]
KAG6588498.1	4.5e-170	74.94	hypothetical protein SDJN03_17063, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022969711.1	1.3e-169	74.47	uncharacterized protein LOC111468788 [Cucurbita maxima]	[more]

BLAST of Spg015728 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O67577	1.9e-17	37.40	tRNA (guanosine(18)-2'-O)-methyltransferase OS=Aquifex aeolicus (strain VF5) OX=...	[more]
P0AGJ3	1.6e-16	34.19	tRNA (guanosine(18)-2'-O)-methyltransferase OS=Escherichia coli O157:H7 OX=83334...	[more]
P0AGJ2	1.6e-16	34.19	tRNA (guanosine(18)-2'-O)-methyltransferase OS=Escherichia coli (strain K12) OX=...	[more]
P0AGJ4	1.6e-16	34.19	tRNA (guanosine(18)-2'-O)-methyltransferase OS=Shigella flexneri OX=623 GN=trmH ...	[more]
Q72GI1	1.9e-14	40.82	tRNA (guanosine(18)-2'-O)-methyltransferase OS=Thermus thermophilus (strain ATCC...	[more]

BLAST of Spg015728 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1F0V5	2.6e-171	75.18	uncharacterized protein LOC111441136 OS=Cucurbita moschata OX=3662 GN=LOC1114411...	[more]
A0A6J1HYK4	6.4e-170	74.47	uncharacterized protein LOC111468788 OS=Cucurbita maxima OX=3661 GN=LOC111468788...	[more]
A0A0A0LC15	1.8e-164	73.24	SpoU_methylase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G824...	[more]
A0A5A7UJ80	4.4e-163	73.24	tRNA (Guanosine(18)-2'-O)-methyltransferase OS=Cucumis melo var. makuwa OX=11946...	[more]
A0A1S3B8M3	4.4e-163	73.24	tRNA (Guanosine(18)-2'-O)-methyltransferase OS=Cucumis melo OX=3656 GN=LOC103486...	[more]

BLAST of Spg015728 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G15390.1	4.6e-104	53.30	tRNA/rRNA methyltransferase (SpoU) family protein	[more]
AT2G19870.1	7.1e-04	22.40	tRNA/rRNA methyltransferase (SpoU) family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR029026	tRNA (guanine-N1-)-methyltransferase, N-terminal	GENE3D	3.40.1280.10		coord: 173..442 e-value: 1.9E-54 score: 186.0
IPR001537	tRNA/rRNA methyltransferase, SpoU type	PFAM	PF00588	SpoU_methylase	coord: 294..400 e-value: 6.7E-25 score: 87.9
IPR033671	tRNA (guanosine(18)-2'-O)-methyltransferase	PANTHER	PTHR43453	RRNA METHYLASE-LIKE	coord: 25..238
IPR033671	tRNA (guanosine(18)-2'-O)-methyltransferase	PANTHER	PTHR43453	RRNA METHYLASE-LIKE	coord: 295..450
IPR033671	tRNA (guanosine(18)-2'-O)-methyltransferase	HAMAP	MF_02060	tRNA_methyltr_TrmH	coord: 179..435 score: 25.504559
IPR033671	tRNA (guanosine(18)-2'-O)-methyltransferase	CDD	cd18092	SpoU-like_TrmH	coord: 181..406 e-value: 1.57322E-70 score: 219.261
None	No IPR available	PANTHER	PTHR43453:SF1	RRNA METHYLASE-LIKE PROTEIN	coord: 295..450
None	No IPR available	PANTHER	PTHR43453:SF1	RRNA METHYLASE-LIKE PROTEIN	coord: 25..238
IPR029028	Alpha/beta knot methyltransferases	SUPERFAMILY	75217	alpha/beta knot	coord: 298..409
IPR029028	Alpha/beta knot methyltransferases	SUPERFAMILY	75217	alpha/beta knot	coord: 181..233

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg015728.1	Spg015728.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0030488	tRNA methylation
biological_process	GO:0006396	RNA processing
molecular_function	GO:0008173	RNA methyltransferase activity
molecular_function	GO:0000049	tRNA binding
molecular_function	GO:0008168	methyltransferase activity
molecular_function	GO:0003723	RNA binding