Spg014956 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg014956
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Descriptionhyoscyamine 6-dioxygenase-like
Locationscaffold3: 42271165 .. 42274094 (-)
RNA-Seq ExpressionSpg014956
SyntenySpg014956
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGAGAGGTAAATATTTTGTCCAACTGTGTGGGTGTTGAATCATCAGTGCCGGAAAGCTATGTGTATCCGTCGGAGATGAGGCCTGGCGTTCCGGTGAAGAAGGAAATTCCGGTGGTTGATTTTACGTCCCACGACCAAGCCCTTCTCGTTCGACAAATCCTCGACGTTTCTCAAGAGTTCGGATTTTTTCAGGTTAGGAAGTACTCATGGATCTTTCGTTATTAATTCTGTCTCTTCCCACATAAACATGTTCGAAAAATATATATAACCATGTTATGTTTTTTTTTTTTTTTTTCTTTTGAGATATGAAACTTCTAGCTCTGATAACAGAAAAAGGGAAAAAATGCATTTTCCCAAACCTTAAAGTTAAACTGAATAAAAAATATACTACCAAACTCAAACCTCAGGAACTAAAAGTGTAATTTTTTTTGTTTCTCGTGATAGGTGATAAACCATGGAGTGTCAAGAAGGTTATTAGAAGAGACGATGAGGATTTTCAAGGAGTTTCATAGCATGTCAACAGCAGACAAAGTGAGGGAATGCTCAAAAGATCCAAACCAAAGCTGCAGAATTTACATTTACACAACCAATGAGGATTATGTAAATGAACACATTCACTCTTGGAGAGATTACGTGCTTTTCAATTGCTATCCTTTGGAGAAATATGTTCATTTTTGGCCTGAAAATCCCCCCAAATACAGGTAAAAAGCTTTCACCCTAATTCACTCTATATATATATATATATATATATTGTTTTTACTTTTAACATTTAGTTAAAGTTAGTGTTTCTTTTAGTGCTTTGTATATTACTTTTAACATGAAATTTTTGTTGGAAAAGTAATTGGATCATGTTAAAGACTAAAGAGAGTACCCCTAAATATTGTCTAATATGCTTAAAAGGGAAGTATTCGGTCTTCTAAAATTGCTTTTCAAAACTACTTTTCAAGGGTTAAAATCATTTTTTTTTCATATTTGAAATCACCCCGTAATGTGTGTTTAATAATTTAAAATCAATTTTGATATGATATAAAAATCACATTTAAAAGTGTAAAATCAAACATGAAATTGATTTTGAATAATTATAGATATGTTTTGGAGTGATTTCGAACATGACAAAAATGATTTTAGCCATTTCAAAAACACTCTCAAACATGCCCTAAGAAGAAAAACAATAGAAAACCGTTTTTAAAATATTGAGCCTTCATTAATTTGTGAATCTCCACTTATTTTTCTTTCTAATAAATATATTTCTAACATTTCTATCTAGAATTTTTCTAGAAAAAATGAAAAAAAAAAAAGATAGGAAAAAAAAGTTAGAGAGAGAAAAATTAGACGGAAAAAATTGTTGGAGAGGGACAGGATGAAGAGAAAAAAATGAGCATTAGAAGGGAAAAAAATAAGTGTTTTGAAAACATATCTATCTTTAATTTTTTTTTAAAAAAAATACTTATAGTTTCGAAAATGTTTATAAAGAACAAAAAAAAACTAAAAACTGAAATGATTATAAAATACCACCCTATTTCCAAAAACGAAAAAGATTATCAAATAGCAAGCTTAAGCTATCAAGTTTAAATCTCATTTTGTTTCTAAATTTTGATATATGTTCCACTTTTGGTAACTTTCAAAACATTTGTTTTACTCTTTAAAATTCAAAATAAATAAATAAATATTTGACTTACATGTATATCGATCATTTTTCTTTTTCAAATCTCCAAATACAGGAAATTAGAGATCTGATTACATTTGCAAATCCTCCTCATGAATATAAATACCCAATTTTTGACCACTTAAATTATGTTCAGATCGACATGCCAATAACCAAAATTAAAATCATGGTTTTATTTTAGAGAGGTGGTTGGAGCATATTGTGTTGCAATGAGAAAGTTGACCATGGAGATATTGGATATTATAAGTGAAGGATTGGGAGTGGAGAGAGAGTACTTGGAGAATGAACTAACTGAAAATTCCAAGGTTGTGAACAACCATTACCCACCATGCCCAAACCCTAGCTTCACTATGGGATTGAAAAAACATTGTGATCCAACCCTTATTAGCATTGTGTTTCAAGATGTCAATGGCCTTCAATATTTCAAGGATGGTGAATGGATTGCTGTTGACCCTATTGATGGTGCCCTCCTGGTTAACATTGGCTACGTTTTACAGGTATGTTTTAATTTGCTTTCGAGATTAACTCGAGTTGGAGTATTTGACATGTGATGACTTTTAGAATAGTGAAAAAGTGCATTTTCGTCGATTAAAAACATTTTGTTTGTCGATCATCTCTTTAAAGTGAGTTTAGACGACGATGGTTTTTTTGGTGTGACTTGTTGGAGAGATAATTGAAAAGTGTTTTTTAGCCCATTGAATTAGAGAGGAGTTAGTAAGAGAAATTGTGTAGAAAGAAAATAGAAATGATTGTAAATGAAGTGTTTAGGCCAAAAAGCACAAAATAAAACAAGAATCAATGGGTGCTTTGACAAACACTTCTAAATGTTTTGACTTTTCCCAAAAGGTCTTTCTTATAAAATGTAGTAAAATATATATGTAAGTACTCTCCAACACACGAAAGTGTTTTATAATTTTTTTTCATATATCCAACACAAATTACCGACTAAATTTGTGACAATCTTCTCTAATAATAATTCTTCAATTTTACGTACAATTGTACGTAGGTGGTCAGCAATGGAAAGCTGAAAGCTGGAGAACACCAAGTTGTGACAAATACAAAGATATCTCGTCAATCTTTAGTGTATCTAGTTTATCCTAAAGATGATGCGACCATGGATCCAGCAAAATGTTTGATAAATGAAGTCAATCATCCACATTATCGTTCTCTCAAGTTTGAAGAGTTCAAGAGAGACTTCTTATCTATGAAGGGTGATAATGAAGCAGTGATGCAATATCTTAGCTCCAATTACCATCCTTAA

mRNA sequence

ATGGGAGAGGTAAATATTTTGTCCAACTGTGTGGGTGTTGAATCATCAGTGCCGGAAAGCTATGTGTATCCGTCGGAGATGAGGCCTGGCGTTCCGGTGAAGAAGGAAATTCCGGTGGTTGATTTTACGTCCCACGACCAAGCCCTTCTCGTTCGACAAATCCTCGACGTTTCTCAAGAGTTCGGATTTTTTCAGGTGATAAACCATGGAGTGTCAAGAAGGTTATTAGAAGAGACGATGAGGATTTTCAAGGAGTTTCATAGCATGTCAACAGCAGACAAAGTGAGGGAATGCTCAAAAGATCCAAACCAAAGCTGCAGAATTTACATTTACACAACCAATGAGGATTATGTAAATGAACACATTCACTCTTGGAGAGATTACGTGCTTTTCAATTGCTATCCTTTGGAGAAATATGTTCATTTTTGGCCTGAAAATCCCCCCAAATACAGAGAGGTGGTTGGAGCATATTGTGTTGCAATGAGAAAGTTGACCATGGAGATATTGGATATTATAAGTGAAGGATTGGGAGTGGAGAGAGAGTACTTGGAGAATGAACTAACTGAAAATTCCAAGGTTGTGAACAACCATTACCCACCATGCCCAAACCCTAGCTTCACTATGGGATTGAAAAAACATTGTGATCCAACCCTTATTAGCATTGTGTTTCAAGATGTCAATGGCCTTCAATATTTCAAGGATGGTGAATGGATTGCTGTTGACCCTATTGATGGTGCCCTCCTGGTTAACATTGGCTACGTTTTACAGGTGGTCAGCAATGGAAAGCTGAAAGCTGGAGAACACCAAGTTGTGACAAATACAAAGATATCTCGTCAATCTTTAGTGTATCTAGTTTATCCTAAAGATGATGCGACCATGGATCCAGCAAAATGTTTGATAAATGAAGTCAATCATCCACATTATCGTTCTCTCAAGTTTGAAGAGTTCAAGAGAGACTTCTTATCTATGAAGGGTGATAATGAAGCAGTGATGCAATATCTTAGCTCCAATTACCATCCTTAA

Coding sequence (CDS)

ATGGGAGAGGTAAATATTTTGTCCAACTGTGTGGGTGTTGAATCATCAGTGCCGGAAAGCTATGTGTATCCGTCGGAGATGAGGCCTGGCGTTCCGGTGAAGAAGGAAATTCCGGTGGTTGATTTTACGTCCCACGACCAAGCCCTTCTCGTTCGACAAATCCTCGACGTTTCTCAAGAGTTCGGATTTTTTCAGGTGATAAACCATGGAGTGTCAAGAAGGTTATTAGAAGAGACGATGAGGATTTTCAAGGAGTTTCATAGCATGTCAACAGCAGACAAAGTGAGGGAATGCTCAAAAGATCCAAACCAAAGCTGCAGAATTTACATTTACACAACCAATGAGGATTATGTAAATGAACACATTCACTCTTGGAGAGATTACGTGCTTTTCAATTGCTATCCTTTGGAGAAATATGTTCATTTTTGGCCTGAAAATCCCCCCAAATACAGAGAGGTGGTTGGAGCATATTGTGTTGCAATGAGAAAGTTGACCATGGAGATATTGGATATTATAAGTGAAGGATTGGGAGTGGAGAGAGAGTACTTGGAGAATGAACTAACTGAAAATTCCAAGGTTGTGAACAACCATTACCCACCATGCCCAAACCCTAGCTTCACTATGGGATTGAAAAAACATTGTGATCCAACCCTTATTAGCATTGTGTTTCAAGATGTCAATGGCCTTCAATATTTCAAGGATGGTGAATGGATTGCTGTTGACCCTATTGATGGTGCCCTCCTGGTTAACATTGGCTACGTTTTACAGGTGGTCAGCAATGGAAAGCTGAAAGCTGGAGAACACCAAGTTGTGACAAATACAAAGATATCTCGTCAATCTTTAGTGTATCTAGTTTATCCTAAAGATGATGCGACCATGGATCCAGCAAAATGTTTGATAAATGAAGTCAATCATCCACATTATCGTTCTCTCAAGTTTGAAGAGTTCAAGAGAGACTTCTTATCTATGAAGGGTGATAATGAAGCAGTGATGCAATATCTTAGCTCCAATTACCATCCTTAA

Protein sequence

MGEVNILSNCVGVESSVPESYVYPSEMRPGVPVKKEIPVVDFTSHDQALLVRQILDVSQEFGFFQVINHGVSRRLLEETMRIFKEFHSMSTADKVRECSKDPNQSCRIYIYTTNEDYVNEHIHSWRDYVLFNCYPLEKYVHFWPENPPKYREVVGAYCVAMRKLTMEILDIISEGLGVEREYLENELTENSKVVNNHYPPCPNPSFTMGLKKHCDPTLISIVFQDVNGLQYFKDGEWIAVDPIDGALLVNIGYVLQVVSNGKLKAGEHQVVTNTKISRQSLVYLVYPKDDATMDPAKCLINEVNHPHYRSLKFEEFKRDFLSMKGDNEAVMQYLSSNYHP
Homology
[Close]
BLAST of Spg014956 vs. NCBI nr
Match: KAG6593078.1 (Hyoscyamine 6-dioxygenase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 478.8 bits (1231), Expect = 3.9e-131
Identity = 223/340 (65.59%), Postives = 280/340 (82.35%), Query Frame = 0 

Query: 1   MGEVNILSNCVGVESSVPESYVYPSEMRPG---VPVKKEIPVVDFTSHDQALLVRQILDV 60
           MGEV  +SN  GV+ SVP  YVYP E RPG   VP+   IPV+D ++ D+ LLVR+ILD 
Sbjct: 1   MGEVKFVSNWEGVQ-SVPPDYVYPPEKRPGNVVVPMANAIPVLDLSTRDRPLLVRKILDA 60

Query: 61  SQEFGFFQVINHGVSRRLLEETMRIFKEFHSMSTADKVRECSKDPNQSCRIYIYTTNEDY 120
           SQEFGFFQVINHGVSRR+LEET RIFK+FH+MS A+KV+ECS+DPN+SCR  +YT++E+Y
Sbjct: 61  SQEFGFFQVINHGVSRRVLEETTRIFKKFHAMSGAEKVKECSRDPNKSCR--VYTSSENY 120

Query: 121 VNEHIHSWRDYVLFNCYPLEKYVHFWPENPPKYREVVGAYCVAMRKLTMEILDIISEGLG 180
             E  H WRD ++FNC+PL+KYVHFWP+NPPKYREVVGAYC+AMR L +EIL+++SEGLG
Sbjct: 121 RKEQTHCWRDALIFNCHPLDKYVHFWPQNPPKYREVVGAYCIAMRTLVLEILELVSEGLG 180

Query: 181 VEREYLENELTENSKVVNNHYPPCPNPSFTMGLKKHCDPTLISIVFQDVNGLQYFKDGEW 240
           + R YL  E++EN  ++ NHYPPCPNPS T+GL +HCDP+LI+I+FQ+VNGLQ  KDG+W
Sbjct: 181 LGRGYLGGEMSENPLMLVNHYPPCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQW 240

Query: 241 IAVDPIDGALLVNIGYVLQVVSNGKLKAGEHQVVTNTKISRQSLVYLVYPKDDATMDPAK 300
           I V PID A +VNIG+VLQV+SNGKLKA EH+VVTN KISRQSL YLVYPKD+ TM+PAK
Sbjct: 241 IGVHPIDNAFVVNIGFVLQVISNGKLKAAEHRVVTNAKISRQSLTYLVYPKDEVTMEPAK 300

Query: 301 CLINEVNHPHYRSLKFEEFKRDFLSMKGDNEAVMQYLSSN 338
           CLINE N P YR+LKF++F+R++L    D +AVM+ + S+
Sbjct: 301 CLINEANPPRYRALKFKDFQRNYLPRASDTKAVMECIGSD 337

[Close]
BLAST of Spg014956 vs. NCBI nr
Match: XP_022959666.1 (hyoscyamine 6-dioxygenase-like [Cucurbita moschata])

HSP 1 Score: 478.8 bits (1231), Expect = 3.9e-131
Identity = 223/340 (65.59%), Postives = 280/340 (82.35%), Query Frame = 0 

Query: 1   MGEVNILSNCVGVESSVPESYVYPSEMRPG---VPVKKEIPVVDFTSHDQALLVRQILDV 60
           MGEV  +SN  GV+ SVP  YVYP E RPG   VP+   IPV+D ++HD+ LLVR+ILD 
Sbjct: 1   MGEVKFVSNWEGVQ-SVPPDYVYPPEKRPGNVVVPMANAIPVLDLSTHDRPLLVRKILDA 60

Query: 61  SQEFGFFQVINHGVSRRLLEETMRIFKEFHSMSTADKVRECSKDPNQSCRIYIYTTNEDY 120
           SQEFGFFQVINHGVSRR+LEET RIFK+FH+MS A+KV+ECS+DPN+SCR  +YT++E+Y
Sbjct: 61  SQEFGFFQVINHGVSRRVLEETTRIFKKFHAMSGAEKVKECSRDPNKSCR--VYTSSENY 120

Query: 121 VNEHIHSWRDYVLFNCYPLEKYVHFWPENPPKYREVVGAYCVAMRKLTMEILDIISEGLG 180
             E  H WRD ++FNC+PL+KYVHFWP+NPPKYREVVGAYC+AMR L +EIL+++SEGLG
Sbjct: 121 RKEQTHCWRDALIFNCHPLDKYVHFWPQNPPKYREVVGAYCIAMRTLVLEILELVSEGLG 180

Query: 181 VEREYLENELTENSKVVNNHYPPCPNPSFTMGLKKHCDPTLISIVFQDVNGLQYFKDGEW 240
           + R YL  E++EN  ++ NHYP CPNPS T+GL +HCDP+LI+I+FQ+VNGLQ  KDG+W
Sbjct: 181 LGRGYLGGEMSENPLMLVNHYPSCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQW 240

Query: 241 IAVDPIDGALLVNIGYVLQVVSNGKLKAGEHQVVTNTKISRQSLVYLVYPKDDATMDPAK 300
           I V PID A +VNIG+VLQV+SNGKLKA EH+VVTN KISRQSL YLVYPKD+ TM+PAK
Sbjct: 241 IGVHPIDNAFVVNIGFVLQVISNGKLKAAEHRVVTNAKISRQSLTYLVYPKDEVTMEPAK 300

Query: 301 CLINEVNHPHYRSLKFEEFKRDFLSMKGDNEAVMQYLSSN 338
           CLINE N P YR+LKF++F+R++L    D +AVM+ + S+
Sbjct: 301 CLINEANPPRYRALKFKDFQRNYLPRASDTKAVMECIGSD 337

[Close]
BLAST of Spg014956 vs. NCBI nr
Match: XP_022143610.1 (hyoscyamine 6-dioxygenase-like [Momordica charantia])

HSP 1 Score: 478.8 bits (1231), Expect = 3.9e-131
Identity = 221/339 (65.19%), Postives = 280/339 (82.60%), Query Frame = 0 

Query: 3   EVNILSNCVGVESSVPESYVYPSEMRPG---VPVKKEIPVVDFTSHDQALLVRQILDVSQ 62
           +VN +SN VGVE SVPESYVYP E RPG   VPV+  IPVVD T HD+A LV +ILD SQ
Sbjct: 4   QVNFVSNWVGVE-SVPESYVYPPEKRPGNVAVPVENAIPVVDLTDHDRAFLVHKILDASQ 63

Query: 63  EFGFFQVINHGVSRRLLEETMRIFKEFHSMSTADKVRECSKDPNQSCRIYIYTTNEDYVN 122
           EFGFFQVINHGVS+ L+EETMR+F+EFH+MS  +K RECSKDPN+SCR  +YT++E++V 
Sbjct: 64  EFGFFQVINHGVSKTLIEETMRVFEEFHAMSGPEKKRECSKDPNRSCR--VYTSSENFVK 123

Query: 123 EHIHSWRDYVLFNCYPLEKYVHFWPENPPKYREVVGAYCVAMRKLTMEILDIISEGLGVE 182
           E  H WRD ++FNC+PL+KYVHFWP+NP  YREVVGAYC+ MRKL +EIL++ISEGLG+ 
Sbjct: 124 EKTHCWRDALIFNCHPLDKYVHFWPQNPSNYREVVGAYCIVMRKLVVEILELISEGLGLG 183

Query: 183 REYLENELTENSKVVNNHYPPCPNPSFTMGLKKHCDPTLISIVFQDVNGLQYFKDGEWIA 242
           + Y  +E++EN  ++ NHYPPCPNPS T+GL +HCDP+LI+I+FQ +NGLQ  KDG+WI 
Sbjct: 184 KGYFGDEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQQINGLQVLKDGQWIG 243

Query: 243 VDPIDGALLVNIGYVLQVVSNGKLKAGEHQVVTNTKISRQSLVYLVYPKDDATMDPAKCL 302
           V P+D A +VNIG+VLQVVSNGKLKAGEH+ V N K+SRQS+ YLVYPKDDATM+PAKC+
Sbjct: 244 VHPVDNAFVVNIGFVLQVVSNGKLKAGEHRAVINAKMSRQSITYLVYPKDDATMEPAKCM 303

Query: 303 INEVNHPHYRSLKFEEFKRDFLSMKGDNEAVMQYLSSNY 339
           INE N   Y SL+F++F+R++L M  D +AV+QY+SS++
Sbjct: 304 INEANPSLYHSLQFKDFQRNYLPMAADTKAVIQYISSDH 339

[Close]
BLAST of Spg014956 vs. NCBI nr
Match: XP_023004374.1 (hyoscyamine 6-dioxygenase-like [Cucurbita maxima])

HSP 1 Score: 477.6 bits (1228), Expect = 8.7e-131
Identity = 223/340 (65.59%), Postives = 278/340 (81.76%), Query Frame = 0 

Query: 1   MGEVNILSNCVGVESSVPESYVYPSEMRPG---VPVKKEIPVVDFTSHDQALLVRQILDV 60
           MGEV  +SN  GV+ SVP  Y+YP E RPG   VP+   IPVVD ++ D+ LLVR+ILD 
Sbjct: 1   MGEVKFVSNWEGVQ-SVPPDYIYPPEKRPGNVVVPMANAIPVVDLSTRDRPLLVRKILDA 60

Query: 61  SQEFGFFQVINHGVSRRLLEETMRIFKEFHSMSTADKVRECSKDPNQSCRIYIYTTNEDY 120
           SQEFGFFQVINHGVSRR+LEET RIFK+FH+MS A+K +ECS+DP +SCR  +YT++E+Y
Sbjct: 61  SQEFGFFQVINHGVSRRVLEETTRIFKKFHAMSGAEKAKECSRDPYKSCR--VYTSSENY 120

Query: 121 VNEHIHSWRDYVLFNCYPLEKYVHFWPENPPKYREVVGAYCVAMRKLTMEILDIISEGLG 180
             E  H WRD ++FNC+PLEKYVHFWP+NPPKYREVVGAYCVAMR L +EIL+++SEGLG
Sbjct: 121 RKEQTHCWRDALIFNCHPLEKYVHFWPQNPPKYREVVGAYCVAMRTLVLEILELVSEGLG 180

Query: 181 VEREYLENELTENSKVVNNHYPPCPNPSFTMGLKKHCDPTLISIVFQDVNGLQYFKDGEW 240
           + R YL +E++EN  ++ NHYPPCPNPS T+GL +HCDP+LI+I+FQ+VNGLQ  KDG+W
Sbjct: 181 LGRGYLASEMSENPLMLVNHYPPCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQW 240

Query: 241 IAVDPIDGALLVNIGYVLQVVSNGKLKAGEHQVVTNTKISRQSLVYLVYPKDDATMDPAK 300
           I V PID A +VNIG+VLQV+SNGKLKA EH+ VTN KISRQSL YLVYPKD+ TM+PAK
Sbjct: 241 IGVHPIDNAFVVNIGFVLQVISNGKLKAAEHRAVTNAKISRQSLTYLVYPKDEVTMEPAK 300

Query: 301 CLINEVNHPHYRSLKFEEFKRDFLSMKGDNEAVMQYLSSN 338
           CLINE N P YR+LKF++F+R++L    D +AVM+ + SN
Sbjct: 301 CLINEANPPRYRALKFKDFQRNYLPRASDTKAVMECIGSN 337

[Close]
BLAST of Spg014956 vs. NCBI nr
Match: XP_023514953.1 (hyoscyamine 6-dioxygenase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 473.0 bits (1216), Expect = 2.2e-129
Identity = 220/340 (64.71%), Postives = 277/340 (81.47%), Query Frame = 0 

Query: 1   MGEVNILSNCVGVESSVPESYVYPSEMRPG---VPVKKEIPVVDFTSHDQALLVRQILDV 60
           MGEV  +SN  GV+ SVP  YVYP E RPG   VP+   IPV+D ++ D+ LLVR+ILD 
Sbjct: 1   MGEVKFVSNWEGVQ-SVPPDYVYPPEKRPGNVVVPMANTIPVLDLSTRDRPLLVRKILDA 60

Query: 61  SQEFGFFQVINHGVSRRLLEETMRIFKEFHSMSTADKVRECSKDPNQSCRIYIYTTNEDY 120
           SQEFGFFQVINHGVS+R+LEET RIFK+FH+MS A+K +ECS+DPN+SCR  +YT++E+Y
Sbjct: 61  SQEFGFFQVINHGVSKRVLEETTRIFKKFHAMSGAEKAKECSRDPNKSCR--VYTSSENY 120

Query: 121 VNEHIHSWRDYVLFNCYPLEKYVHFWPENPPKYREVVGAYCVAMRKLTMEILDIISEGLG 180
             E  H WRD ++FNC+PL+KYVHFWP+NPPKYREVVGAYCV MR L +EIL+++SEGLG
Sbjct: 121 RKEQTHCWRDALIFNCHPLDKYVHFWPQNPPKYREVVGAYCVEMRALVLEILELVSEGLG 180

Query: 181 VEREYLENELTENSKVVNNHYPPCPNPSFTMGLKKHCDPTLISIVFQDVNGLQYFKDGEW 240
           + R YL  E++EN  ++ NHYPPCPNPS T+GL +HCDP+LI+I+FQ+VNGLQ  KDG+W
Sbjct: 181 LGRGYLGGEMSENPLMLVNHYPPCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQW 240

Query: 241 IAVDPIDGALLVNIGYVLQVVSNGKLKAGEHQVVTNTKISRQSLVYLVYPKDDATMDPAK 300
           I V PID A +VNIG+VLQV+SNGKLKA EH+ VTN KISRQSL YLVYPKD+ TM+PAK
Sbjct: 241 IGVHPIDNAFVVNIGFVLQVISNGKLKAAEHRAVTNAKISRQSLTYLVYPKDEVTMEPAK 300

Query: 301 CLINEVNHPHYRSLKFEEFKRDFLSMKGDNEAVMQYLSSN 338
           CLINE N P YR+LKF++F+R++L    D +AVM+ + S+
Sbjct: 301 CLINEANPPRYRALKFKDFQRNYLPRASDTKAVMECIGSD 337

[Close]
BLAST of Spg014956 vs. ExPASy Swiss-Prot
Match: Q9FLV0 (Protein DOWNY MILDEW RESISTANCE 6 OS=Arabidopsis thaliana OX=3702 GN=DMR6 PE=1 SV=1)

HSP 1 Score: 249.2 bits (635), Expect = 6.6e-65
Identity = 127/332 (38.25%), Postives = 204/332 (61.45%), Query Frame = 0 

Query: 15  SSVPESYVYPSEMRP---GVPVKKEIPVVDFTSHDQALLVRQILDVSQEFGFFQVINHGV 74
           +++PE+YV P   RP    V   ++ P++D +S D++ L++QI      FGFFQVINHGV
Sbjct: 13  TTLPENYVRPISDRPRLSEVSQLEDFPLIDLSSTDRSFLIQQIHQACARFGFFQVINHGV 72

Query: 75  SRRLLEETMRIFKEFHSMSTADKVRECSKDPNQSCRIYIYTTNEDYVNEHIHSWRDYVLF 134
           ++++++E + + +EF SMS  +K++  S DP ++ R+   +T+ +   E +++WRDY+  
Sbjct: 73  NKQIIDEMVSVAREFFSMSMEEKMKLYSDDPTKTTRL---STSFNVKKEEVNNWRDYLRL 132

Query: 135 NCYPLEKYVHFWPENPPKYREVVGAYCVAMRKLTMEILDIISEGLGVEREYLENELTENS 194
           +CYP+ KYV+ WP NPP ++E+V  Y   +R++  +I ++ISE LG+E++Y++  L E  
Sbjct: 133 HCYPIHKYVNEWPSNPPSFKEIVSKYSREVREVGFKIEELISESLGLEKDYMKKVLGEQG 192

Query: 195 K-VVNNHYPPCPNPSFTMGLKKHCDPTLISIVFQD--VNGLQYFKDGEWIAVDPIDGALL 254
           + +  N+YPPCP P  T GL  H DP  ++I+ QD  V GLQ   DG+W AV+P   A +
Sbjct: 193 QHMAVNYYPPCPEPELTYGLPAHTDPNALTILLQDTTVCGLQILIDGQWFAVNPHPDAFV 252

Query: 255 VNIGYVLQVVSNGKLKAGEHQVVTNTKISRQSLVYLVYPKDDATMDPAKCL---INEVNH 314
           +NIG  LQ +SNG  K+  H+ VTNT+  R S+   + P D A M PAK L    ++   
Sbjct: 253 INIGDQLQALSNGVYKSVWHRAVTNTENPRLSVASFLCPADCAVMSPAKPLWEAEDDETK 312

Query: 315 PHYRSLKFEEFKRDFLSMKGDNEAVMQYLSSN 338
           P Y+   + E+ + F S   D E  ++   +N
Sbjct: 313 PVYKDFTYAEYYKKFWSRNLDQEHCLENFLNN 341

[Close]
BLAST of Spg014956 vs. ExPASy Swiss-Prot
Match: P24397 (Hyoscyamine 6-dioxygenase OS=Hyoscyamus niger OX=4079 GN=H6H PE=1 SV=1)

HSP 1 Score: 243.0 bits (619), Expect = 4.7e-63
Identity = 133/325 (40.92%), Postives = 193/325 (59.38%), Query Frame = 0 

Query: 16  SVPESYVYPSEMR--PGVPVKKEIPVVDFTSHDQALLVRQILDVSQEFGFFQVINHGVSR 75
           SV ES++ P + R    VPV  ++P++D   H   LLV+QI    Q+FG FQVINHG   
Sbjct: 12  SVSESFIAPLQKRAEKDVPVGNDVPIIDLQQHHH-LLVQQITKACQDFGLFQVINHGFPE 71

Query: 76  RLLEETMRIFKEFHSMSTADKVR-----ECSK-DPNQSCRIYIYTTNEDYVNEHIHSWRD 135
            L+ ETM + KEF ++   +K +     E +K +     +  +Y   E   NE    W+D
Sbjct: 72  ELMLETMEVCKEFFALPAEEKEKFKPKGEAAKFELPLEQKAKLYVEGEQLSNEEFLYWKD 131

Query: 136 YVLFNCYPLEK-YVHFWPENPPKYREVVGAYCVAMRKLTMEILDIISEGLGVEREYLENE 195
            +   C+PL++  V+ WPE P KYREVV  Y V +RKLTM +LD I EGLG++  Y +NE
Sbjct: 132 TLAHGCHPLDQDLVNSWPEKPAKYREVVAKYSVEVRKLTMRMLDYICEGLGLKLGYFDNE 191

Query: 196 LTENSKVVNNHYPPCPNPSFTMGLKKHCDPTLISIVFQDVNGLQYF--KDGEWIAVDPID 255
           L++   ++ N+YPPCP+PS T+G   H D  LI+++ QD+ GLQ    KD  WIAV PI 
Sbjct: 192 LSQIQMMLTNYYPPCPDPSSTLGSGGHYDGNLITLLQQDLPGLQQLIVKDATWIAVQPIP 251

Query: 256 GALLVNIGYVLQVVSNGKLKAGEHQVVTNTKISRQSLVYLVYPKDDATMDPAKCLINEVN 315
            A +VN+G  L+V++N K +   H+VVT+    R S+  L+ P    T++PAK L+N+ N
Sbjct: 252 TAFVVNLGLTLKVITNEKFEGSIHRVVTDPTRDRVSIATLIGPDYSCTIEPAKELLNQDN 311

Query: 316 HPHYRSLKFEEFKRDFLSMKGDNEA 330
            P Y+   + EF   +LS K D ++
Sbjct: 312 PPLYKPYSYSEFADIYLSDKSDYDS 335

[Close]
BLAST of Spg014956 vs. ExPASy Swiss-Prot
Match: Q8W2X5 (Flavanone 3-dioxygenase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=F3H-2 PE=1 SV=1)

HSP 1 Score: 239.6 bits (610), Expect = 5.2e-62
Identity = 121/326 (37.12%), Postives = 186/326 (57.06%), Query Frame = 0 

Query: 13  VESSVPESYVYPSEMRPGVPV---KKEIPVVDFTSHDQALLVRQILDVSQEFGFFQVINH 72
           V  ++P  YV P   RP + +      IPVVD  S D+A +V  + D  +  GFFQV+NH
Sbjct: 16  VHDTMPGKYVRPESQRPRLDLVVSDARIPVVDLASPDRAAVVSAVGDACRTHGFFQVVNH 75

Query: 73  GVSRRLLEETMRIFKEFHSMSTADKVRECSKDPNQSCRIYIYTTNEDYVNEHIHSWRDYV 132
           G+   L+   M + +EF  +   +K +  S DP +  R+   +T+ +   E +H+WRDY+
Sbjct: 76  GIDAALIASVMEVGREFFRLPAEEKAKLYSDDPAKKIRL---STSFNVRKETVHNWRDYL 135

Query: 133 LFNCYPLEKYVHFWPENPPKYREVVGAYCVAMRKLTMEILDIISEGLGVEREYLENELTE 192
             +CYPL ++V  WP NPP ++E++G YC  +R+L   + + ISE LG+E  Y+   L E
Sbjct: 136 RLHCYPLHQFVPDWPSNPPSFKEIIGTYCTEVRELGFRLYEAISESLGLEGGYMRETLGE 195

Query: 193 NSK-VVNNHYPPCPNPSFTMGLKKHCDPTLISIVFQD--VNGLQYFKDGEWIAVDPIDGA 252
             + +  N+YP CP P  T GL  H DP  ++I+  D  V GLQ   DG+WIAV+P  GA
Sbjct: 196 QEQHMAVNYYPQCPEPELTYGLPAHTDPNALTILLMDDQVAGLQVLNDGKWIAVNPQPGA 255

Query: 253 LLVNIGYVLQVVSNGKLKAGEHQVVTNTKISRQSLVYLVYPKDDATMDPAKCLINEVNHP 312
           L++NIG  LQ +SNGK ++  H+ V N+   R S+   + P +   + PAK LI + +  
Sbjct: 256 LVINIGDQLQALSNGKYRSVWHRAVVNSDRERMSVASFLCPCNSVELGPAKKLITDDSPA 315

Query: 313 HYRSLKFEEFKRDFLSMKGDNEAVMQ 333
            YR+  ++E+ + F S   D E  ++
Sbjct: 316 VYRNYTYDEYYKKFWSRNLDQEHCLE 338

[Close]
BLAST of Spg014956 vs. ExPASy Swiss-Prot
Match: Q9ZSA8 (Protein DMR6-LIKE OXYGENASE 1 OS=Arabidopsis thaliana OX=3702 GN=DLO1 PE=1 SV=1)

HSP 1 Score: 214.5 bits (545), Expect = 1.8e-54
Identity = 109/310 (35.16%), Postives = 184/310 (59.35%), Query Frame = 0 

Query: 17  VPESYVYPSEMRPGVPVKK----EIPVVDFT---SHDQALLVRQILDVSQEFGFFQVINH 76
           +P +YV P   RP +   +     IP++D       ++A++V+Q+      +GFFQ+ NH
Sbjct: 20  IPSNYVRPISDRPNLSEVESSGDSIPLIDLRDLHGPNRAVIVQQLASACSTYGFFQIKNH 79

Query: 77  GVSRRLLEETMRIFKEFHSMSTADKVRECSKDPNQSCRIYIYTTNEDYVNEHIHSWRDYV 136
           GV    + +   + +EF     +++V+  S DP ++ R+   +T+ +   + + +WRD++
Sbjct: 80  GVPDTTVNKMQTVAREFFHQPESERVKHYSADPTKTTRL---STSFNVGADKVLNWRDFL 139

Query: 137 LFNCYPLEKYVHFWPENPPKYREVVGAYCVAMRKLTMEILDIISEGLGVEREYLENELTE 196
             +C+P+E ++  WP +P  +REV   Y  ++R L + +L+ ISE LG+E +++ N L +
Sbjct: 140 RLHCFPIEDFIEEWPSSPISFREVTAEYATSVRALVLRLLEAISESLGLESDHISNILGK 199

Query: 197 NSK-VVNNHYPPCPNPSFTMGLKKHCDPTLISIVFQD-VNGLQYFKDGEWIAVDPIDGAL 256
           +++ +  N+YPPCP P  T GL  H DPT+I+++ QD V+GLQ FKD +W+AV PI    
Sbjct: 200 HAQHMAFNYYPPCPEPELTYGLPGHKDPTVITVLLQDQVSGLQVFKDDKWVAVSPIPNTF 259

Query: 257 LVNIGYVLQVVSNGKLKAGEHQVVTNTKISRQSLVYLVYPKDDATMDPAKCLINEVNH-P 316
           +VNIG  +QV+SN K K+  H+ V NT+  R S+    +P  DA + PA  L+NE +   
Sbjct: 260 IVNIGDQMQVISNDKYKSVLHRAVVNTENERLSIPTFYFPSTDAVIGPAHELVNEQDSLA 319

[Close]
BLAST of Spg014956 vs. ExPASy Swiss-Prot
Match: Q9ZSA7 (Protein DMR6-LIKE OXYGENASE 2 OS=Arabidopsis thaliana OX=3702 GN=DLO2 PE=2 SV=1)

HSP 1 Score: 210.7 bits (535), Expect = 2.6e-53
Identity = 114/337 (33.83%), Postives = 188/337 (55.79%), Query Frame = 0 

Query: 6   ILSNCVGVESSVPESYVYPSEMRPGVPVKK----EIPVVDFTSHD-----QALLVRQILD 65
           ++S+   V   VP +YV P   RP +   +     IP++D   HD     +A ++ Q   
Sbjct: 7   LVSDIASVVDHVPSNYVRPVSDRPKMSEVQTSGDSIPLIDL--HDLHGPNRADIINQFAH 66

Query: 66  VSQEFGFFQVINHGVSRRLLEETMRIFKEFHSMSTADKVRECSKDPNQSCRIYIYTTNED 125
                GFFQ+ NHGV    +++ M   +EF   S +++V+  S D  ++ R+   +T+ +
Sbjct: 67  ACSSCGFFQIKNHGVPEETIKKMMNAAREFFRQSESERVKHYSADTKKTTRL---STSFN 126

Query: 126 YVNEHIHSWRDYVLFNCYPLEKYVHFWPENPPKYREVVGAYCVAMRKLTMEILDIISEGL 185
              E + +WRD++  +CYP+E +++ WP  P  +REV   Y  ++R L + +L+ ISE L
Sbjct: 127 VSKEKVSNWRDFLRLHCYPIEDFINEWPSTPISFREVTAEYATSVRALVLTLLEAISESL 186

Query: 186 GVEREYLENELTENSK-VVNNHYPPCPNPSFTMGLKKHCDPTLISIVFQD-VNGLQYFKD 245
           G+ ++ + N + ++ + +  N+YP CP P  T GL  H D  LI+++ QD V+GLQ FKD
Sbjct: 187 GLAKDRVSNTIGKHGQHMAINYYPRCPQPELTYGLPGHKDANLITVLLQDEVSGLQVFKD 246

Query: 246 GEWIAVDPIDGALLVNIGYVLQVVSNGKLKAGEHQVVTNTKISRQSLVYLVYPKDDATMD 305
           G+WIAV+P+    +VN+G  +QV+SN K K+  H+ V N+ + R S+     P +DA + 
Sbjct: 247 GKWIAVNPVPNTFIVNLGDQMQVISNEKYKSVLHRAVVNSDMERISIPTFYCPSEDAVIS 306

Query: 306 PAKCLINEVNHPH--YRSLKFEEFKRDFLSMKGDNEA 330
           PA+ LINE       YR+  + E+   F     D E+
Sbjct: 307 PAQELINEEEDSPAIYRNFTYAEYFEKFWDTAFDTES 338

[Close]
BLAST of Spg014956 vs. ExPASy TrEMBL
Match: A0A6J1CP90 (hyoscyamine 6-dioxygenase-like OS=Momordica charantia OX=3673 GN=LOC111013466 PE=3 SV=1)

HSP 1 Score: 478.8 bits (1231), Expect = 1.9e-131
Identity = 221/339 (65.19%), Postives = 280/339 (82.60%), Query Frame = 0 

Query: 3   EVNILSNCVGVESSVPESYVYPSEMRPG---VPVKKEIPVVDFTSHDQALLVRQILDVSQ 62
           +VN +SN VGVE SVPESYVYP E RPG   VPV+  IPVVD T HD+A LV +ILD SQ
Sbjct: 4   QVNFVSNWVGVE-SVPESYVYPPEKRPGNVAVPVENAIPVVDLTDHDRAFLVHKILDASQ 63

Query: 63  EFGFFQVINHGVSRRLLEETMRIFKEFHSMSTADKVRECSKDPNQSCRIYIYTTNEDYVN 122
           EFGFFQVINHGVS+ L+EETMR+F+EFH+MS  +K RECSKDPN+SCR  +YT++E++V 
Sbjct: 64  EFGFFQVINHGVSKTLIEETMRVFEEFHAMSGPEKKRECSKDPNRSCR--VYTSSENFVK 123

Query: 123 EHIHSWRDYVLFNCYPLEKYVHFWPENPPKYREVVGAYCVAMRKLTMEILDIISEGLGVE 182
           E  H WRD ++FNC+PL+KYVHFWP+NP  YREVVGAYC+ MRKL +EIL++ISEGLG+ 
Sbjct: 124 EKTHCWRDALIFNCHPLDKYVHFWPQNPSNYREVVGAYCIVMRKLVVEILELISEGLGLG 183

Query: 183 REYLENELTENSKVVNNHYPPCPNPSFTMGLKKHCDPTLISIVFQDVNGLQYFKDGEWIA 242
           + Y  +E++EN  ++ NHYPPCPNPS T+GL +HCDP+LI+I+FQ +NGLQ  KDG+WI 
Sbjct: 184 KGYFGDEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQQINGLQVLKDGQWIG 243

Query: 243 VDPIDGALLVNIGYVLQVVSNGKLKAGEHQVVTNTKISRQSLVYLVYPKDDATMDPAKCL 302
           V P+D A +VNIG+VLQVVSNGKLKAGEH+ V N K+SRQS+ YLVYPKDDATM+PAKC+
Sbjct: 244 VHPVDNAFVVNIGFVLQVVSNGKLKAGEHRAVINAKMSRQSITYLVYPKDDATMEPAKCM 303

Query: 303 INEVNHPHYRSLKFEEFKRDFLSMKGDNEAVMQYLSSNY 339
           INE N   Y SL+F++F+R++L M  D +AV+QY+SS++
Sbjct: 304 INEANPSLYHSLQFKDFQRNYLPMAADTKAVIQYISSDH 339

[Close]
BLAST of Spg014956 vs. ExPASy TrEMBL
Match: A0A6J1H5H6 (hyoscyamine 6-dioxygenase-like OS=Cucurbita moschata OX=3662 GN=LOC111460677 PE=3 SV=1)

HSP 1 Score: 478.8 bits (1231), Expect = 1.9e-131
Identity = 223/340 (65.59%), Postives = 280/340 (82.35%), Query Frame = 0 

Query: 1   MGEVNILSNCVGVESSVPESYVYPSEMRPG---VPVKKEIPVVDFTSHDQALLVRQILDV 60
           MGEV  +SN  GV+ SVP  YVYP E RPG   VP+   IPV+D ++HD+ LLVR+ILD 
Sbjct: 1   MGEVKFVSNWEGVQ-SVPPDYVYPPEKRPGNVVVPMANAIPVLDLSTHDRPLLVRKILDA 60

Query: 61  SQEFGFFQVINHGVSRRLLEETMRIFKEFHSMSTADKVRECSKDPNQSCRIYIYTTNEDY 120
           SQEFGFFQVINHGVSRR+LEET RIFK+FH+MS A+KV+ECS+DPN+SCR  +YT++E+Y
Sbjct: 61  SQEFGFFQVINHGVSRRVLEETTRIFKKFHAMSGAEKVKECSRDPNKSCR--VYTSSENY 120

Query: 121 VNEHIHSWRDYVLFNCYPLEKYVHFWPENPPKYREVVGAYCVAMRKLTMEILDIISEGLG 180
             E  H WRD ++FNC+PL+KYVHFWP+NPPKYREVVGAYC+AMR L +EIL+++SEGLG
Sbjct: 121 RKEQTHCWRDALIFNCHPLDKYVHFWPQNPPKYREVVGAYCIAMRTLVLEILELVSEGLG 180

Query: 181 VEREYLENELTENSKVVNNHYPPCPNPSFTMGLKKHCDPTLISIVFQDVNGLQYFKDGEW 240
           + R YL  E++EN  ++ NHYP CPNPS T+GL +HCDP+LI+I+FQ+VNGLQ  KDG+W
Sbjct: 181 LGRGYLGGEMSENPLMLVNHYPSCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQW 240

Query: 241 IAVDPIDGALLVNIGYVLQVVSNGKLKAGEHQVVTNTKISRQSLVYLVYPKDDATMDPAK 300
           I V PID A +VNIG+VLQV+SNGKLKA EH+VVTN KISRQSL YLVYPKD+ TM+PAK
Sbjct: 241 IGVHPIDNAFVVNIGFVLQVISNGKLKAAEHRVVTNAKISRQSLTYLVYPKDEVTMEPAK 300

Query: 301 CLINEVNHPHYRSLKFEEFKRDFLSMKGDNEAVMQYLSSN 338
           CLINE N P YR+LKF++F+R++L    D +AVM+ + S+
Sbjct: 301 CLINEANPPRYRALKFKDFQRNYLPRASDTKAVMECIGSD 337

[Close]
BLAST of Spg014956 vs. ExPASy TrEMBL
Match: A0A6J1KW36 (hyoscyamine 6-dioxygenase-like OS=Cucurbita maxima OX=3661 GN=LOC111497716 PE=3 SV=1)

HSP 1 Score: 477.6 bits (1228), Expect = 4.2e-131
Identity = 223/340 (65.59%), Postives = 278/340 (81.76%), Query Frame = 0 

Query: 1   MGEVNILSNCVGVESSVPESYVYPSEMRPG---VPVKKEIPVVDFTSHDQALLVRQILDV 60
           MGEV  +SN  GV+ SVP  Y+YP E RPG   VP+   IPVVD ++ D+ LLVR+ILD 
Sbjct: 1   MGEVKFVSNWEGVQ-SVPPDYIYPPEKRPGNVVVPMANAIPVVDLSTRDRPLLVRKILDA 60

Query: 61  SQEFGFFQVINHGVSRRLLEETMRIFKEFHSMSTADKVRECSKDPNQSCRIYIYTTNEDY 120
           SQEFGFFQVINHGVSRR+LEET RIFK+FH+MS A+K +ECS+DP +SCR  +YT++E+Y
Sbjct: 61  SQEFGFFQVINHGVSRRVLEETTRIFKKFHAMSGAEKAKECSRDPYKSCR--VYTSSENY 120

Query: 121 VNEHIHSWRDYVLFNCYPLEKYVHFWPENPPKYREVVGAYCVAMRKLTMEILDIISEGLG 180
             E  H WRD ++FNC+PLEKYVHFWP+NPPKYREVVGAYCVAMR L +EIL+++SEGLG
Sbjct: 121 RKEQTHCWRDALIFNCHPLEKYVHFWPQNPPKYREVVGAYCVAMRTLVLEILELVSEGLG 180

Query: 181 VEREYLENELTENSKVVNNHYPPCPNPSFTMGLKKHCDPTLISIVFQDVNGLQYFKDGEW 240
           + R YL +E++EN  ++ NHYPPCPNPS T+GL +HCDP+LI+I+FQ+VNGLQ  KDG+W
Sbjct: 181 LGRGYLASEMSENPLMLVNHYPPCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQW 240

Query: 241 IAVDPIDGALLVNIGYVLQVVSNGKLKAGEHQVVTNTKISRQSLVYLVYPKDDATMDPAK 300
           I V PID A +VNIG+VLQV+SNGKLKA EH+ VTN KISRQSL YLVYPKD+ TM+PAK
Sbjct: 241 IGVHPIDNAFVVNIGFVLQVISNGKLKAAEHRAVTNAKISRQSLTYLVYPKDEVTMEPAK 300

Query: 301 CLINEVNHPHYRSLKFEEFKRDFLSMKGDNEAVMQYLSSN 338
           CLINE N P YR+LKF++F+R++L    D +AVM+ + SN
Sbjct: 301 CLINEANPPRYRALKFKDFQRNYLPRASDTKAVMECIGSN 337

[Close]
BLAST of Spg014956 vs. ExPASy TrEMBL
Match: A0A0A0K5H3 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G378370 PE=3 SV=1)

HSP 1 Score: 468.8 bits (1205), Expect = 2.0e-128
Identity = 215/339 (63.42%), Postives = 275/339 (81.12%), Query Frame = 0 

Query: 2   GEVNILSNCVGVESSVPESYVYPSEMRPG---VPVKKEIPVVDFTSHDQALLVRQILDVS 61
           G    +SN   VE SVPESYVYP E RPG   VP+ K IPV+D +  D+ LLVR+ILD S
Sbjct: 4   GGAKFVSNWETVE-SVPESYVYPPEKRPGNIVVPMAKAIPVIDLSIRDRTLLVRKILDAS 63

Query: 62  QEFGFFQVINHGVSRRLLEETMRIFKEFHSMSTADKVRECSKDPNQSCRIYIYTTNEDYV 121
           +EFGFFQ+INHGVS+++ EETMRIFKEFH+MS  +K +ECSKDPN+SCR  +YT++E+Y 
Sbjct: 64  KEFGFFQIINHGVSKKVSEETMRIFKEFHAMSGPEKAKECSKDPNRSCR--VYTSSENYT 123

Query: 122 NEHIHSWRDYVLFNCYPLEKYVHFWPENPPKYREVVGAYCVAMRKLTMEILDIISEGLGV 181
            E +H WRD ++FNC+PLEKYVHFWP+ PPKYREVVGAYC+AMRKL +EIL+++SEGLG+
Sbjct: 124 KEQVHCWRDALIFNCHPLEKYVHFWPQKPPKYREVVGAYCMAMRKLVLEILELMSEGLGL 183

Query: 182 EREYLENELTENSKVVNNHYPPCPNPSFTMGLKKHCDPTLISIVFQDVNGLQYFKDGEWI 241
            + Y   E++EN  ++ NHYPPCPNPS T+GL +HCDP+LI+I+FQDVNGLQ  KDG+WI
Sbjct: 184 GKGYFGGEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGQWI 243

Query: 242 AVDPIDGALLVNIGYVLQVVSNGKLKAGEHQVVTNTKISRQSLVYLVYPKDDATMDPAKC 301
            V PID A +VNIG+VLQ+++NGKL+A EH+ VTN+K SRQSL YLVYPKD+A M+PAKC
Sbjct: 244 GVQPIDNAFVVNIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEAMMEPAKC 303

Query: 302 LINEVNHPHYRSLKFEEFKRDFLSMKGDNEAVMQYLSSN 338
           +INE N P YRSL F++F+R++L    D +AVMQY+ +N
Sbjct: 304 MINEANPPRYRSLNFKDFQRNYLPRAVDTKAVMQYIETN 339

[Close]
BLAST of Spg014956 vs. ExPASy TrEMBL
Match: A0A1S3BR20 (hyoscyamine 6-dioxygenase-like OS=Cucumis melo OX=3656 GN=LOC103492610 PE=3 SV=1)

HSP 1 Score: 468.4 bits (1204), Expect = 2.6e-128
Identity = 218/339 (64.31%), Postives = 276/339 (81.42%), Query Frame = 0 

Query: 2   GEVNILSNCVGVESSVPESYVYPSEMRPG---VPVKKEIPVVDFTSHDQALLVRQILDVS 61
           G+   +SN    E SVPESYVYP E RPG   VP+ K IPV+D +  D+ALLVR+IL  S
Sbjct: 4   GDAKFVSNWETAE-SVPESYVYPPEKRPGNVVVPMAKAIPVIDLSICDRALLVRKILGAS 63

Query: 62  QEFGFFQVINHGVSRRLLEETMRIFKEFHSMSTADKVRECSKDPNQSCRIYIYTTNEDYV 121
           +E GFFQ+INHGVS+R+ E TMRIFKEFH+MS  +K +ECSKDPN+SCR  +YT++E+Y 
Sbjct: 64  KESGFFQIINHGVSKRVSEATMRIFKEFHAMSGPEKAKECSKDPNRSCR--VYTSSENYK 123

Query: 122 NEHIHSWRDYVLFNCYPLEKYVHFWPENPPKYREVVGAYCVAMRKLTMEILDIISEGLGV 181
            E IH WRD ++FNC+PLEKYVHFWP+NPPKYREVVGAYCVAMRKL +EIL+++SEGLG+
Sbjct: 124 KEQIHCWRDALIFNCHPLEKYVHFWPQNPPKYREVVGAYCVAMRKLVLEILELMSEGLGL 183

Query: 182 EREYLENELTENSKVVNNHYPPCPNPSFTMGLKKHCDPTLISIVFQDVNGLQYFKDGEWI 241
            + YL  E++EN  ++ NHYPPCPNPS T+GL +HCDP+LI+I+FQDVNGLQ  KDG+WI
Sbjct: 184 GKGYLGGEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGQWI 243

Query: 242 AVDPIDGALLVNIGYVLQVVSNGKLKAGEHQVVTNTKISRQSLVYLVYPKDDATMDPAKC 301
            V PID A +VNIG+VLQ+++NGKL+A EH+ VTN+K SRQSL YLVYPKD+ATM+PAKC
Sbjct: 244 GVQPIDKAFVVNIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEATMEPAKC 303

Query: 302 LINEVNHPHYRSLKFEEFKRDFLSMKGDNEAVMQYLSSN 338
           +INE N P YRSL F++F+R++L    D +AVMQY+ +N
Sbjct: 304 MINEANPPRYRSLNFKDFQRNYLPRAVDTKAVMQYIETN 339

[Close]
BLAST of Spg014956 vs. TAIR 10
Match: AT5G24530.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 249.2 bits (635), Expect = 4.7e-66
Identity = 127/332 (38.25%), Postives = 204/332 (61.45%), Query Frame = 0 

Query: 15  SSVPESYVYPSEMRP---GVPVKKEIPVVDFTSHDQALLVRQILDVSQEFGFFQVINHGV 74
           +++PE+YV P   RP    V   ++ P++D +S D++ L++QI      FGFFQVINHGV
Sbjct: 13  TTLPENYVRPISDRPRLSEVSQLEDFPLIDLSSTDRSFLIQQIHQACARFGFFQVINHGV 72

Query: 75  SRRLLEETMRIFKEFHSMSTADKVRECSKDPNQSCRIYIYTTNEDYVNEHIHSWRDYVLF 134
           ++++++E + + +EF SMS  +K++  S DP ++ R+   +T+ +   E +++WRDY+  
Sbjct: 73  NKQIIDEMVSVAREFFSMSMEEKMKLYSDDPTKTTRL---STSFNVKKEEVNNWRDYLRL 132

Query: 135 NCYPLEKYVHFWPENPPKYREVVGAYCVAMRKLTMEILDIISEGLGVEREYLENELTENS 194
           +CYP+ KYV+ WP NPP ++E+V  Y   +R++  +I ++ISE LG+E++Y++  L E  
Sbjct: 133 HCYPIHKYVNEWPSNPPSFKEIVSKYSREVREVGFKIEELISESLGLEKDYMKKVLGEQG 192

Query: 195 K-VVNNHYPPCPNPSFTMGLKKHCDPTLISIVFQD--VNGLQYFKDGEWIAVDPIDGALL 254
           + +  N+YPPCP P  T GL  H DP  ++I+ QD  V GLQ   DG+W AV+P   A +
Sbjct: 193 QHMAVNYYPPCPEPELTYGLPAHTDPNALTILLQDTTVCGLQILIDGQWFAVNPHPDAFV 252

Query: 255 VNIGYVLQVVSNGKLKAGEHQVVTNTKISRQSLVYLVYPKDDATMDPAKCL---INEVNH 314
           +NIG  LQ +SNG  K+  H+ VTNT+  R S+   + P D A M PAK L    ++   
Sbjct: 253 INIGDQLQALSNGVYKSVWHRAVTNTENPRLSVASFLCPADCAVMSPAKPLWEAEDDETK 312

Query: 315 PHYRSLKFEEFKRDFLSMKGDNEAVMQYLSSN 338
           P Y+   + E+ + F S   D E  ++   +N
Sbjct: 313 PVYKDFTYAEYYKKFWSRNLDQEHCLENFLNN 341

[Close]
BLAST of Spg014956 vs. TAIR 10
Match: AT4G10500.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 214.5 bits (545), Expect = 1.3e-55
Identity = 109/310 (35.16%), Postives = 184/310 (59.35%), Query Frame = 0 

Query: 17  VPESYVYPSEMRPGVPVKK----EIPVVDFT---SHDQALLVRQILDVSQEFGFFQVINH 76
           +P +YV P   RP +   +     IP++D       ++A++V+Q+      +GFFQ+ NH
Sbjct: 20  IPSNYVRPISDRPNLSEVESSGDSIPLIDLRDLHGPNRAVIVQQLASACSTYGFFQIKNH 79

Query: 77  GVSRRLLEETMRIFKEFHSMSTADKVRECSKDPNQSCRIYIYTTNEDYVNEHIHSWRDYV 136
           GV    + +   + +EF     +++V+  S DP ++ R+   +T+ +   + + +WRD++
Sbjct: 80  GVPDTTVNKMQTVAREFFHQPESERVKHYSADPTKTTRL---STSFNVGADKVLNWRDFL 139

Query: 137 LFNCYPLEKYVHFWPENPPKYREVVGAYCVAMRKLTMEILDIISEGLGVEREYLENELTE 196
             +C+P+E ++  WP +P  +REV   Y  ++R L + +L+ ISE LG+E +++ N L +
Sbjct: 140 RLHCFPIEDFIEEWPSSPISFREVTAEYATSVRALVLRLLEAISESLGLESDHISNILGK 199

Query: 197 NSK-VVNNHYPPCPNPSFTMGLKKHCDPTLISIVFQD-VNGLQYFKDGEWIAVDPIDGAL 256
           +++ +  N+YPPCP P  T GL  H DPT+I+++ QD V+GLQ FKD +W+AV PI    
Sbjct: 200 HAQHMAFNYYPPCPEPELTYGLPGHKDPTVITVLLQDQVSGLQVFKDDKWVAVSPIPNTF 259

Query: 257 LVNIGYVLQVVSNGKLKAGEHQVVTNTKISRQSLVYLVYPKDDATMDPAKCLINEVNH-P 316
           +VNIG  +QV+SN K K+  H+ V NT+  R S+    +P  DA + PA  L+NE +   
Sbjct: 260 IVNIGDQMQVISNDKYKSVLHRAVVNTENERLSIPTFYFPSTDAVIGPAHELVNEQDSLA 319

[Close]
BLAST of Spg014956 vs. TAIR 10
Match: AT4G10490.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 210.7 bits (535), Expect = 1.9e-54
Identity = 114/337 (33.83%), Postives = 188/337 (55.79%), Query Frame = 0 

Query: 6   ILSNCVGVESSVPESYVYPSEMRPGVPVKK----EIPVVDFTSHD-----QALLVRQILD 65
           ++S+   V   VP +YV P   RP +   +     IP++D   HD     +A ++ Q   
Sbjct: 7   LVSDIASVVDHVPSNYVRPVSDRPKMSEVQTSGDSIPLIDL--HDLHGPNRADIINQFAH 66

Query: 66  VSQEFGFFQVINHGVSRRLLEETMRIFKEFHSMSTADKVRECSKDPNQSCRIYIYTTNED 125
                GFFQ+ NHGV    +++ M   +EF   S +++V+  S D  ++ R+   +T+ +
Sbjct: 67  ACSSCGFFQIKNHGVPEETIKKMMNAAREFFRQSESERVKHYSADTKKTTRL---STSFN 126

Query: 126 YVNEHIHSWRDYVLFNCYPLEKYVHFWPENPPKYREVVGAYCVAMRKLTMEILDIISEGL 185
              E + +WRD++  +CYP+E +++ WP  P  +REV   Y  ++R L + +L+ ISE L
Sbjct: 127 VSKEKVSNWRDFLRLHCYPIEDFINEWPSTPISFREVTAEYATSVRALVLTLLEAISESL 186

Query: 186 GVEREYLENELTENSK-VVNNHYPPCPNPSFTMGLKKHCDPTLISIVFQD-VNGLQYFKD 245
           G+ ++ + N + ++ + +  N+YP CP P  T GL  H D  LI+++ QD V+GLQ FKD
Sbjct: 187 GLAKDRVSNTIGKHGQHMAINYYPRCPQPELTYGLPGHKDANLITVLLQDEVSGLQVFKD 246

Query: 246 GEWIAVDPIDGALLVNIGYVLQVVSNGKLKAGEHQVVTNTKISRQSLVYLVYPKDDATMD 305
           G+WIAV+P+    +VN+G  +QV+SN K K+  H+ V N+ + R S+     P +DA + 
Sbjct: 247 GKWIAVNPVPNTFIVNLGDQMQVISNEKYKSVLHRAVVNSDMERISIPTFYCPSEDAVIS 306

Query: 306 PAKCLINEVNHPH--YRSLKFEEFKRDFLSMKGDNEA 330
           PA+ LINE       YR+  + E+   F     D E+
Sbjct: 307 PAQELINEEEDSPAIYRNFTYAEYFEKFWDTAFDTES 338

[Close]
BLAST of Spg014956 vs. TAIR 10
Match: AT2G44800.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 188.7 bits (478), Expect = 7.5e-48
Identity = 105/327 (32.11%), Postives = 173/327 (52.91%), Query Frame = 0 

Query: 17  VPESYVYPSEMRPGV-----PVKKEIPVVDFTSHDQ----ALLVRQILDVSQEFGFFQVI 76
           VP+ YV P   RP +       +  +PV+D +   Q    +L + +I    +EFGFFQVI
Sbjct: 27  VPDRYVLPPSQRPALGSSLGTSETTLPVIDLSLLHQPFLRSLAIHEISMACKEFGFFQVI 86

Query: 77  NHGVSRRLLEETMRIFKEFHSMSTADKVRECSKDPNQSCRIYIYTTNEDYVNEHIHSWRD 136
           NHG+   ++ + +    +F  +   +K+   S + ++  R   Y T+ ++  + +H WRD
Sbjct: 87  NHGIPSSVVNDALDAATQFFDLPVEEKMLLVSANVHEPVR---YGTSLNHSTDRVHYWRD 146

Query: 137 YVLFNCYPLEKYVHFWPENPPKYREVVGAYCVAMRKLTMEILDIISEGLGVEREYLENEL 196
           ++    +PL K++  WP NPP Y++ VG Y  A   L  ++++ ISE LG+E+ YL+ E+
Sbjct: 147 FIKHYSHPLSKWIDMWPSNPPCYKDKVGKYAEATHLLHKQLIEAISESLGLEKNYLQEEI 206

Query: 197 TENSKVVN-NHYPPCPNPSFTMGLKKHCDPTLISIVFQDVNGLQYFK-DGEWIAVDPIDG 256
            E S+V+  N YP CP P   +G+  H D + ++I+ Q   GLQ    +  W+ V  I+G
Sbjct: 207 EEGSQVMAVNCYPACPEPEMALGMPPHSDFSSLTILLQSSKGLQIMDCNKNWVCVPYIEG 266

Query: 257 ALLVNIGYVLQVVSNGKLKAGEHQVVTNTKISRQSLVYLVYPKDDATMDPAKCLINEVNH 316
           AL+V +G  ++V+SNG  K+  H+V  N ++ R S   L        + PA  L+N  N 
Sbjct: 267 ALIVQLGDQVEVMSNGIYKSVIHRVTVNKEVKRLSFASLHSLPLHKKISPAPKLVNPNNA 326

Query: 317 PHYRSLKFEEFKRDFLSMKGDNEAVMQ 333
           P Y    F     DFL+    N+ + +
Sbjct: 327 PAYGEFSF----NDFLNYISSNDFIQE 346

[Close]
BLAST of Spg014956 vs. TAIR 10
Match: AT3G60290.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 176.4 bits (446), Expect = 3.9e-44
Identity = 106/318 (33.33%), Postives = 166/318 (52.20%), Query Frame = 0 

Query: 17  VPESYVYPSEMRP--GVPVKK---EIPVVDFT-SHDQAL---LVRQILDVSQEFGFFQVI 76
           VP  Y+ P   RP  G  +      +PV+D +  HD  L   ++ +I    + FGFFQVI
Sbjct: 27  VPTRYILPPSQRPMLGPSIGTSTINLPVIDLSFLHDPLLRLCVIHEIELACKGFGFFQVI 86

Query: 77  NHGVSRRLLEETMRIFKEFHSMSTADKVRECSKDPNQSCRIYIYTTNEDYVNEHIHSWRD 136
           NHG+S  ++++       F  +   +K+   S +  +  R   Y T+ ++  + +H WRD
Sbjct: 87  NHGISSAVVKDAQDSATRFFDLPADEKMHLVSDNFQEPVR---YGTSINHSTDRVHYWRD 146

Query: 137 YVLFNCYPLEKYVHFWPENPPKYREVVGAYCVAMRKLTMEILDIISEGLGVEREYLENEL 196
           ++    +PL  +++ WP NPP Y+E VG Y  A   L  ++++ ISE LG+E+ YL+ E+
Sbjct: 147 FIKHYSHPLSNWINLWPSNPPCYKEKVGKYAEATHVLHKQLIEAISESLGLEKNYLQEEI 206

Query: 197 TENSKVVN-NHYPPCPNPSFTMGLKKHCDPTLISIVFQDVNGLQYFKD--GEWIAVDPID 256
            E S+V+  N YP CP P   +G+  H D   ++I+ Q   GLQ  KD    W+ V  I+
Sbjct: 207 EEGSQVMAVNCYPACPEPEIALGMPPHSDYGSLTILLQSSEGLQ-IKDCNNNWVCVPYIE 266

Query: 257 GALLVNIGYVLQVVSNGKLKAGEHQVVTNTKISRQSLVYLVYPKDDATMDPAKCLINEVN 316
           GAL+V +G  ++V+SNG  K+  H+V  N    R S   L        + PA  L+NE  
Sbjct: 267 GALIVQLGDQVEVMSNGIYKSVVHRVTVNKDYKRLSFASLHSLPMHKKISPATQLVNENK 326

Query: 317 HPHYRSLKFEEFKRDFLS 323
              Y    F +F  D++S
Sbjct: 327 PAAYGEFSFNDF-LDYIS 339

The following BLAST results are available for this feature:
BLAST of Spg014956 vs. NCBI nr
Match NameE-valueIdentityDescription
KAG6593078.13.9e-13165.59Hyoscyamine 6-dioxygenase, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022959666.13.9e-13165.59hyoscyamine 6-dioxygenase-like [Cucurbita moschata][more]
XP_022143610.13.9e-13165.19hyoscyamine 6-dioxygenase-like [Momordica charantia][more]
XP_023004374.18.7e-13165.59hyoscyamine 6-dioxygenase-like [Cucurbita maxima][more]
XP_023514953.12.2e-12964.71hyoscyamine 6-dioxygenase-like [Cucurbita pepo subsp. pepo][more]
BLAST of Spg014956 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9FLV06.6e-6538.25Protein DOWNY MILDEW RESISTANCE 6 OS=Arabidopsis thaliana OX=3702 GN=DMR6 PE=1 S... [more]
P243974.7e-6340.92Hyoscyamine 6-dioxygenase OS=Hyoscyamus niger OX=4079 GN=H6H PE=1 SV=1[more]
Q8W2X55.2e-6237.12Flavanone 3-dioxygenase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=F3H-2 PE=1... [more]
Q9ZSA81.8e-5435.16Protein DMR6-LIKE OXYGENASE 1 OS=Arabidopsis thaliana OX=3702 GN=DLO1 PE=1 SV=1[more]
Q9ZSA72.6e-5333.83Protein DMR6-LIKE OXYGENASE 2 OS=Arabidopsis thaliana OX=3702 GN=DLO2 PE=2 SV=1[more]
BLAST of Spg014956 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1CP901.9e-13165.19hyoscyamine 6-dioxygenase-like OS=Momordica charantia OX=3673 GN=LOC111013466 PE... [more]
A0A6J1H5H61.9e-13165.59hyoscyamine 6-dioxygenase-like OS=Cucurbita moschata OX=3662 GN=LOC111460677 PE=... [more]
A0A6J1KW364.2e-13165.59hyoscyamine 6-dioxygenase-like OS=Cucurbita maxima OX=3661 GN=LOC111497716 PE=3 ... [more]
A0A0A0K5H32.0e-12863.42Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G... [more]
A0A1S3BR202.6e-12864.31hyoscyamine 6-dioxygenase-like OS=Cucumis melo OX=3656 GN=LOC103492610 PE=3 SV=1[more]
BLAST of Spg014956 vs. TAIR 10
Match NameE-valueIdentityDescription
AT5G24530.14.7e-6638.252-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT4G10500.11.3e-5535.162-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT4G10490.11.9e-5433.832-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT2G44800.17.5e-4832.112-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT3G60290.13.9e-4433.332-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR026992Non-haem dioxygenase N-terminal domainPFAMPF14226DIOX_Ncoord: 37..145
e-value: 2.2E-15
score: 57.4
IPR044861Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domainPFAMPF031712OG-FeII_Oxycoord: 192..284
e-value: 3.2E-21
score: 75.7
IPR027443Isopenicillin N synthase-like superfamilyGENE3D2.60.120.330coord: 4..328
e-value: 2.8E-80
score: 272.1
NoneNo IPR availablePANTHERPTHR47991:SF311-AMINOCYCLOPROPANE-1-CARBOXYLATE OXIDASEcoord: 5..334
NoneNo IPR availablePANTHERPTHR47991OXOGLUTARATE/IRON-DEPENDENT DIOXYGENASEcoord: 5..334
NoneNo IPR availableSUPERFAMILY51197Clavaminate synthase-likecoord: 14..325
IPR005123Oxoglutarate/iron-dependent dioxygenasePROSITEPS51471FE2OG_OXYcoord: 188..287
score: 11.137102

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg014956.1Spg014956.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0051213 dioxygenase activity
molecular_function GO:0046872 metal ion binding