Spg014911 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg014911
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionRNase H domain-containing protein
Locationscaffold3: 46416822 .. 46418030 (+)
RNA-Seq ExpressionSpg014911
SyntenySpg014911
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGAGCGTTTTGTGCGGAGCTTATTTATAGAGTGGGCCTGAAGGCGCGTGGACCCCATGGAGACTTGGTGTCCAATGCGACGGCGAAAGGTGGCGCGCAAAATGCGGCCACGTGGAGGGTAGATGGAATGGGAAAGAAAAAAAAAAAAGTAAGAATGTTTTCGTCCCCAGTTTTAAACTTGTATTAATAAGTCCTTTTCAATTGGTTACCCATCTTTAAATTTCATTTTAGTTTGGTATCTTATTTTAGTCTCTATGGATATCGAAAAAATAAAAAGACTCCCTATAAATACTAACTTACCTGATAAAATCATATGACATTATAATAGAACAGGAAAATATTCAGTTAATAGTGGATATAAATTGTTTATGAACTCCAAAATTAATGAAGCATCGTCTAGTTCAAATCCAATGTGTAATGTTTGGAAAAGACTATAGAATCTAAGAATATATAATAAGATAAAACTTTTCTGTTGGAAAACATTGAATAATTTCATCCCTACCCATTTAAACCTGTTGAACAGGGGGATAAATTCTTATGAGTATTGTCCTATTTGCCATAAATACCCTGAATCTACTGACCATACTTTGTTTGCATGTGATTGAATAAAGGAGATCTAGATGCATACCTTCAACAATATGTTTTCTTGGAGGAAAATTTCAGTAACAGTTTGGTGGATCGTTGGCTGAAGATAAACTCCTGATGTTCCTCACGACGAAAGAGTTCCCTCAGTCGATGAAAAATGTTGATGGATTAGAAACTATTTAGAGGAGTACAAAAAAATGAATTTAGAAGATCCGTTACCTGCCATTCTGTCGGAAGATTCTCTGTCGATGAATTCAAATGATTGTTGGATTCATCCCCTGGTAATTTTTGGAAGATGAATGTGGATGCTTCGTGGGGCACAAATCCTCCCTCATATCCTCCCTCATCTGGAATAGGAGCCATTTTTAGGGATTTAGCAGGTTCTATCAGAGGGACAACAAGCTCTTACATTGATGTGGATTATACGGGTCCAAATGTTGAATTATGGGCCATAGCTGAAGGCTTGAAGATGGCTATAAGTTTGGGCTGTGAAAGATTGATAGTGGAATCTGATAGTCTAATGGCCATAAATCTCATTACAAAAAAATCAGATCAATTGGGAGAAGTAGAAGCTTTTCTAGAATTGATTTGGGCAATGATCCCATCATTTAGAGAGAGATAA

mRNA sequence

ATGAGAGCGTTTTGTGCGGAGCTTATTTATAGAGTGGGCCTGAAGGCGCGTGGACCCCATGGAGACTTGGTGTCCAATGCGACGGCGAAAGGTGGCGCGCAAAATGCGGCCACGTGGAGGATGAATGTGGATGCTTCGTGGGGCACAAATCCTCCCTCATATCCTCCCTCATCTGGAATAGGAGCCATTTTTAGGGATTTAGCAGGTTCTATCAGAGGGACAACAAGCTCTTACATTGATGTGGATTATACGGGTCCAAATGTTGAATTATGGGCCATAGCTGAAGGCTTGAAGATGGCTATAAGTTTGGGCTGTGAAAGATTGATAGTGGAATCTGATAGTCTAATGGCCATAAATCTCATTACAAAAAAATCAGATCAATTGGGAGAAGTAGAAGCTTTTCTAGAATTGATTTGGGCAATGATCCCATCATTTAGAGAGAGATAA

Coding sequence (CDS)

ATGAGAGCGTTTTGTGCGGAGCTTATTTATAGAGTGGGCCTGAAGGCGCGTGGACCCCATGGAGACTTGGTGTCCAATGCGACGGCGAAAGGTGGCGCGCAAAATGCGGCCACGTGGAGGATGAATGTGGATGCTTCGTGGGGCACAAATCCTCCCTCATATCCTCCCTCATCTGGAATAGGAGCCATTTTTAGGGATTTAGCAGGTTCTATCAGAGGGACAACAAGCTCTTACATTGATGTGGATTATACGGGTCCAAATGTTGAATTATGGGCCATAGCTGAAGGCTTGAAGATGGCTATAAGTTTGGGCTGTGAAAGATTGATAGTGGAATCTGATAGTCTAATGGCCATAAATCTCATTACAAAAAAATCAGATCAATTGGGAGAAGTAGAAGCTTTTCTAGAATTGATTTGGGCAATGATCCCATCATTTAGAGAGAGATAA

Protein sequence

MRAFCAELIYRVGLKARGPHGDLVSNATAKGGAQNAATWRMNVDASWGTNPPSYPPSSGIGAIFRDLAGSIRGTTSSYIDVDYTGPNVELWAIAEGLKMAISLGCERLIVESDSLMAINLITKKSDQLGEVEAFLELIWAMIPSFRER
Homology
BLAST of Spg014911 vs. NCBI nr
Match: KAF7823154.1 (putative WRKY transcription factor 31 [Senna tora])

HSP 1 Score: 62.0 bits (149), Expect = 5.0e-06
Identity = 33/83 (39.76%), Postives = 48/83 (57.83%), Query Frame = 0

Query: 39  WRMNVDASWGTNPPSYPPSSGIGAIFRDLAGSIRGTTSSYIDVDYTGPNVELWAIAEGLK 98
           W++N D +   NP  +     IG I R+  G+     S Y+    T    ELWAI+ G+K
Sbjct: 41  WKLNTDGACSGNPGPF----AIGGIIRNQLGNWISGYSGYVGTG-TALKAELWAISSGIK 100

Query: 99  MAISLGCERLIVESDSLMAINLI 122
           +A +LGC+ L +ESDSL+A NL+
Sbjct: 101 LASNLGCKHLWIESDSLLACNLL 118

BLAST of Spg014911 vs. NCBI nr
Match: KAF7844846.1 (reverse transcriptase [Senna tora])

HSP 1 Score: 62.0 bits (149), Expect = 5.0e-06
Identity = 34/83 (40.96%), Postives = 48/83 (57.83%), Query Frame = 0

Query: 39  WRMNVDASWGTNPPSYPPSSGIGAIFRDLAGSIRGTTSSYIDVDYTGPNVELWAIAEGLK 98
           W++N + +   NP  +     IG + R+  G+     SS+I  D T    ELWAI  GL 
Sbjct: 855 WKLNTNGACSGNPGPF----AIGGLIRNHNGTFISGFSSFIGYD-TAFTAELWAITVGLN 914

Query: 99  MAISLGCERLIVESDSLMAINLI 122
           MA+SL C  + VESDSL+A+NL+
Sbjct: 915 MALSLNCNYICVESDSLLAVNLL 932

BLAST of Spg014911 vs. NCBI nr
Match: KAF7830619.1 (Trimethylguanosine synthase [Senna tora])

HSP 1 Score: 61.2 bits (147), Expect = 8.5e-06
Identity = 45/123 (36.59%), Postives = 60/123 (48.78%), Query Frame = 0

Query: 8   LIYRVGLKARG-------PHGDLV--SNATAKGGAQNAATWRMNVDASWGTNPPSYPPSS 67
           +I R GL A          H  L+  +N   K     A  W++N D +   NP  +    
Sbjct: 688 MILRKGLAAAAEFFHLNPQHSSLLNSNNIQIKWHPPIANWWKLNTDGACSGNPGPF---- 747

Query: 68  GIGAIFRDLAGSIRGTTSSYIDVDYTGPNVELWAIAEGLKMAISLGCERLIVESDSLMAI 122
            IG I RD  GS     SSYI    T    ELWAI+ GL++A+SL C  + VESDSL+A+
Sbjct: 748 AIGGIIRDHNGSFISGYSSYIGFG-TALKAELWAISVGLRIALSLNCNFIHVESDSLLAV 805

BLAST of Spg014911 vs. NCBI nr
Match: XP_038902513.1 (uncharacterized protein LOC120089172 [Benincasa hispida])

HSP 1 Score: 60.8 bits (146), Expect = 1.1e-05
Identity = 36/100 (36.00%), Postives = 54/100 (54.00%), Query Frame = 0

Query: 40  RMNVDASWGTNPPSYPPSSGIGAIFRDLAGSIRGTTSSYIDVDYTGPNVELWAIAEGLKM 99
           ++NVDA+W  +    P SSG  AI RD  GS++    S ID  Y  P  E + + +GL++
Sbjct: 65  KLNVDAAWKFS----PYSSGFSAIIRDNQGSLKVVQISSIDAVYPPPLAEAFVVLQGLRL 124

Query: 100 AISLGCERLIVESDSLMAINLITKKSDQLGEVEAFLELIW 140
              +  +++IV+SD   AI+L  K       V  +LE IW
Sbjct: 125 TSKMNFKKIIVKSDCSGAIDLFLKILISDSSVRVWLEEIW 160

BLAST of Spg014911 vs. NCBI nr
Match: KAF7841164.1 (reverse transcriptase [Senna tora])

HSP 1 Score: 60.1 bits (144), Expect = 1.9e-05
Identity = 35/87 (40.23%), Postives = 48/87 (55.17%), Query Frame = 0

Query: 39  WRMNVDASWGTNPPSYPPSSGIGAIFRDLAGSIRGTTSSYIDVDYTGPNVELWAIAEGLK 98
           W++N D ++  NP  +     IG I RD  G+     S +I  D T   V+LWAI  GLK
Sbjct: 352 WKLNCDGAYQGNPGPF----SIGGILRDAHGNWISGYSGFIG-DGTALEVKLWAITMGLK 411

Query: 99  MAISLGCERLIVESDSLMAINLITKKS 126
           +A  L C  L +E+DSL+A NL+   S
Sbjct: 412 VATILNCNLLWIETDSLLAFNLLMDNS 433

BLAST of Spg014911 vs. ExPASy TrEMBL
Match: A0A803QE56 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 56.2 bits (134), Expect = 1.3e-04
Identity = 35/127 (27.56%), Postives = 60/127 (47.24%), Query Frame = 0

Query: 20   HGDLVSNATAKGGAQ--NAATWRMNVDA------SWGTNPPSYPPSSGIGAIFRDLAGSI 79
            HG  V+    +G  +  +   W++NVDA       W          SG+  + +D +G +
Sbjct: 1357 HGPTVTTGRGRGAQRVPHTGVWKINVDAGVKRGRGW----------SGVSCVIQDSSGCV 1416

Query: 80   RGTTSSYIDVDYTGPNVELWAIAEGLKMAISLGCERLIVESDSLMAINLITKKSDQLGEV 139
               +S+ +  +Y   + EL AI  GL+  I  G ++  +ESD L AI LI  K +   ++
Sbjct: 1417 SYASSTILHREYQPLHAELMAIHGGLQTGIQRGVQQFYIESDCLEAIQLIQNKEESCRDI 1473

BLAST of Spg014911 vs. ExPASy TrEMBL
Match: A0A803P6S3 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 56.2 bits (134), Expect = 1.3e-04
Identity = 38/126 (30.16%), Postives = 61/126 (48.41%), Query Frame = 0

Query: 17  RGPHGDLVSNATAKGGAQNAATWRMNVDASWGTNPPSYPPSSGIGAIFRDLAGSIRGTTS 76
           RG  G     +  +     A   ++NVDA  G     +   SG+G + RD AG +R   +
Sbjct: 16  RGGSGSKEGRSGQRWTPPGANQVKINVDA--GVKQGGH--CSGLGCVARDRAGVVRYAAA 75

Query: 77  SYIDVDYTGPNVELWAIAEGLKMAISLGCERLIVESDSLMAINLITKKSDQLGEVEAFLE 136
           + +        +EL AI  GL++ I    E+  +ESD L A+NLI KK D   +++  L 
Sbjct: 76  TVVQQALPPLQLELMAICRGLQLGIQQRYEQFSIESDCLQAVNLIQKKEDGCRDIDGLLH 135

Query: 137 LIWAMI 143
            I +++
Sbjct: 136 QIRSLM 137

BLAST of Spg014911 vs. ExPASy TrEMBL
Match: A0A1R3GKQ5 (Reverse transcriptase OS=Corchorus capsularis OX=210143 GN=CCACVL1_25420 PE=4 SV=1)

HSP 1 Score: 55.8 bits (133), Expect = 1.7e-04
Identity = 36/106 (33.96%), Postives = 55/106 (51.89%), Query Frame = 0

Query: 33   AQNAATWRMNVDASWGTNPPSYPPSSGIGAIFRDLAGSIRGTTSSYIDVDYTGPNVELWA 92
            A +  TW++N DAS+     S    +G+G + RD  G I  + +  +       + E+ A
Sbjct: 1467 APSPGTWKINTDASFS----SSTGDAGLGVVIRDYEGKILVSGARILFFVADSLHAEVHA 1526

Query: 93   IAEGLKMAISLGCERLIVESDSLMAINLITKKSDQLGEVEAFLELI 139
            I  G ++A+  G  R I+ESDSL+AI+ I KK   L E    +E I
Sbjct: 1527 ILFGFELALEHGITRCIIESDSLLAIHEINKKETVLWEGGLLIEEI 1568

BLAST of Spg014911 vs. ExPASy TrEMBL
Match: A0A6P5RF39 (uncharacterized protein LOC110744764 OS=Prunus avium OX=42229 GN=LOC110744764 PE=4 SV=1)

HSP 1 Score: 55.8 bits (133), Expect = 1.7e-04
Identity = 35/100 (35.00%), Postives = 53/100 (53.00%), Query Frame = 0

Query: 40  RMNVDASWGTNPPSYPPSSGIGAIFRDLAGSIRGTTSSYIDVDYTGPNVELWAIAEGLKM 99
           ++NVD +W  N         +G + RD  G++ G  SS+  V  +   VE  AI EGLK+
Sbjct: 96  KINVDGAWHKN----TMHGAVGCVIRDNRGNLIGGASSF-TVRSSPEEVEAEAILEGLKL 155

Query: 100 AISLGCERLIVESDSLMAINLITKKSDQLGEVEAFLELIW 140
           A ++  + ++VESDS + I  I+KK   L  +   L  IW
Sbjct: 156 ARTMHLQHIVVESDSKVVIESISKKKLMLWRIFPLLAEIW 190

BLAST of Spg014911 vs. ExPASy TrEMBL
Match: A0A4V6ABH7 (RNase H domain-containing protein OS=Populus alba OX=43335 GN=D5086_0000038960 PE=4 SV=1)

HSP 1 Score: 55.1 bits (131), Expect = 2.9e-04
Identity = 35/92 (38.04%), Postives = 54/92 (58.70%), Query Frame = 0

Query: 57  SSGIGAIFRDLAG-SIRGTTSSYIDVDYTGPNVELWAIAEGLKMAISLGCERLIVESDSL 116
           ++GIG +FRD  G +I G  S     D     VELWAI  GL +A++L  + + VESDSL
Sbjct: 278 NAGIGGLFRDYEGNAICGFVSKASGHDIF--LVELWAIWRGLVLALNLRIQVVWVESDSL 337

Query: 117 MAINLITKKSDQLGEVEAFLELIWAMIPSFRE 148
            A+N I ++    G+ +A L+ I  ++  F++
Sbjct: 338 SAVNTINRQQPYSGKADACLKQIRLLLKKFKK 367

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAF7823154.15.0e-0639.76putative WRKY transcription factor 31 [Senna tora][more]
KAF7844846.15.0e-0640.96reverse transcriptase [Senna tora][more]
KAF7830619.18.5e-0636.59Trimethylguanosine synthase [Senna tora][more]
XP_038902513.11.1e-0536.00uncharacterized protein LOC120089172 [Benincasa hispida][more]
KAF7841164.11.9e-0540.23reverse transcriptase [Senna tora][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A803QE561.3e-0427.56Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1[more]
A0A803P6S31.3e-0430.16Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1[more]
A0A1R3GKQ51.7e-0433.96Reverse transcriptase OS=Corchorus capsularis OX=210143 GN=CCACVL1_25420 PE=4 SV... [more]
A0A6P5RF391.7e-0435.00uncharacterized protein LOC110744764 OS=Prunus avium OX=42229 GN=LOC110744764 PE... [more]
A0A4V6ABH72.9e-0438.04RNase H domain-containing protein OS=Populus alba OX=43335 GN=D5086_0000038960 P... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 56..147
e-value: 1.6E-15
score: 57.0
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 37..147
e-value: 2.5E-10
score: 42.5
NoneNo IPR availablePANTHERPTHR47723OS05G0353850 PROTEINcoord: 35..147
IPR044730Ribonuclease H-like domain, plant typeCDDcd06222RNase_H_likecoord: 42..147
e-value: 1.11562E-15
score: 66.5688
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 38..124

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg014911.1Spg014911.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity