Spg014773 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg014773 vs. NCBI nr
Match: KAG7011773.1 (putative 26S proteasome non-ATPase regulatory subunit 3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 877.1 bits (2265), Expect = 8.1e-251
Identity = 468/532 (87.97%), Postives = 484/532 (90.98%), Query Frame = 0

Query: 33  GFHTEFAKTTQDAEMKELPAPVSSSSAPTSPSTLQRKKFYLHFVMLVEFLEDLVLKFVND 92
           GFHTEFAK TQDAEMKELP P+SSS+APTSPSTLQ                         
Sbjct: 32  GFHTEFAKMTQDAEMKELPPPISSSAAPTSPSTLQH------------------------ 91

Query: 93  FFQSDLKEIVSLIETGAYAREVRRIVRAIRLTMALRRKLKASVLSSFLNFSLPPGSDVHT 152
                LKEIVSL+ETGAYAREVRRIVRAIRLTMALR+KLKASVLS FLNF+LPPGSDVHT
Sbjct: 92  -----LKEIVSLLETGAYAREVRRIVRAIRLTMALRQKLKASVLSPFLNFALPPGSDVHT 151

Query: 153 RLSSFIPKEDVYEMDVDSATQAL----VKHPLPEIEIFCYLIVLIFLIDQKKYDEAKACA 212
           RLSSFIPKED YEMD+DS T+AL    VKHPLPEIEIFCYL++L+FLIDQKKY EAKACA
Sbjct: 152 RLSSFIPKEDDYEMDIDSETKALTQAPVKHPLPEIEIFCYLLLLVFLIDQKKYGEAKACA 211

Query: 213 SASIARLKNLNRRTVDVLASRLYFYYSLSYELTGDLAEIRGNLLALHRIGTLRHDELGQE 272
           SASIARLKNLNRRTVDVL+SRLYFYYSLSYELTGDLAEIRGNLLALHRI TLRHDELGQE
Sbjct: 212 SASIARLKNLNRRTVDVLSSRLYFYYSLSYELTGDLAEIRGNLLALHRIATLRHDELGQE 271

Query: 273 TLLNLLLRNYLHYNLYDQAEKLRSKAPRFEAHSNQQASLSALFCRYLFYLGKIRTIQLEY 332
           TLLNLLLRNYLHYNLYDQAEKLRSKAPRFEAHSNQQ      FCRYLFYLGKIRTIQLEY
Sbjct: 272 TLLNLLLRNYLHYNLYDQAEKLRSKAPRFEAHSNQQ------FCRYLFYLGKIRTIQLEY 331

Query: 333 TDAKESLLQAARKAPIAALGFRVQCNKWAIIVRLLLGEIPERTVFMQKGMETALRPYFEL 392
           TDAKESLLQAARKAPIAALGFRVQCNKWAIIVRLLLGEIPERTVFMQKGMETALRPYFEL
Sbjct: 332 TDAKESLLQAARKAPIAALGFRVQCNKWAIIVRLLLGEIPERTVFMQKGMETALRPYFEL 391

Query: 393 TNAVRIGDLELFRTVAEKFSSTFSSDRTNNLIVRLRHNVIRTGLRNISISYSRISLADVA 452
           TNAVRIGDLELFRTVA+KFSS+FSSDRTNNLIVRLRHNVIRTGLRNISISYSRISLAD+A
Sbjct: 392 TNAVRIGDLELFRTVADKFSSSFSSDRTNNLIVRLRHNVIRTGLRNISISYSRISLADIA 451

Query: 453 KKLRLDSENPVADAESIVSKAIRDGAIDATVDHANGWMVSKETGDIYSTNEPQIAFNSRI 512
           KKLRLDSENP+ADAESIVSKAIRDGAIDATVDHA GWMVSKETGDIYSTNEPQIAFNSRI
Sbjct: 452 KKLRLDSENPIADAESIVSKAIRDGAIDATVDHAKGWMVSKETGDIYSTNEPQIAFNSRI 511

Query: 513 AFCLNMHNEAVRALRFPPNSHKEKESAEKRRERQQQEQELAKHIAEEDEDDF 561
           AFCLNMHNEAVRALRFPPNSHKEKESAEKRRERQQQEQELAKHIAEED+DDF
Sbjct: 512 AFCLNMHNEAVRALRFPPNSHKEKESAEKRRERQQQEQELAKHIAEEDDDDF 528

BLAST of Spg014773 vs. NCBI nr
Match: XP_038888951.1 (probable 26S proteasome non-ATPase regulatory subunit 3 [Benincasa hispida] >XP_038888952.1 probable 26S proteasome non-ATPase regulatory subunit 3 [Benincasa hispida])

HSP 1 Score: 873.2 bits (2255), Expect = 1.2e-249
Identity = 468/519 (90.17%), Postives = 477/519 (91.91%), Query Frame = 0

Query: 42  TQDAEMKELPAPVSSSSAPTSPSTLQRKKFYLHFVMLVEFLEDLVLKFVNDFFQSDLKEI 101
           TQ+AEMKELPAP+SSSSAPTSPSTLQ                              LKEI
Sbjct: 2   TQNAEMKELPAPISSSSAPTSPSTLQH-----------------------------LKEI 61

Query: 102 VSLIETGAYAREVRRIVRAIRLTMALRRKLKASVLSSFLNFSLPPGSDVHTRLSSFIPKE 161
           VSL+ETGAYAREVRRIVRAIRLTMALRRKLK SVLSSFLNF+LP GSDVHTRLSSFIPKE
Sbjct: 62  VSLLETGAYAREVRRIVRAIRLTMALRRKLKTSVLSSFLNFALPSGSDVHTRLSSFIPKE 121

Query: 162 DVYEMDVDSATQALVKHPLPEIEIFCYLIVLIFLIDQKKYDEAKACASASIARLKNLNRR 221
           D YEMDVDSATQA  KHPLPEIEIFCYLI+LIFLIDQKKYDEAKACASASIARLKNLNRR
Sbjct: 122 DDYEMDVDSATQASSKHPLPEIEIFCYLILLIFLIDQKKYDEAKACASASIARLKNLNRR 181

Query: 222 TVDVLASRLYFYYSLSYELTGDLAEIRGNLLALHRIGTLRHDELGQETLLNLLLRNYLHY 281
           TVDVLASRLYFYYSLSYE+TGDLAEIRGNLLALHRI TLRHDELGQETLLNLLLRNYLHY
Sbjct: 182 TVDVLASRLYFYYSLSYEVTGDLAEIRGNLLALHRIATLRHDELGQETLLNLLLRNYLHY 241

Query: 282 NLYDQAEKLRSKAPRFEAHSNQQASLSALFCRYLFYLGKIRTIQLEYTDAKESLLQAARK 341
           NLYDQAEKLRSKAPRFEAHSNQQ      FCRYLFYLGKIRTIQLEYTDAKESLLQAARK
Sbjct: 242 NLYDQAEKLRSKAPRFEAHSNQQ------FCRYLFYLGKIRTIQLEYTDAKESLLQAARK 301

Query: 342 APIAALGFRVQCNKWAIIVRLLLGEIPERTVFMQKGMETALRPYFELTNAVRIGDLELFR 401
           APIAALGFRVQCNKWAI+VRLLLGEIPERTVFMQKGMETALRPYFELTNAVRIGDLELFR
Sbjct: 302 APIAALGFRVQCNKWAILVRLLLGEIPERTVFMQKGMETALRPYFELTNAVRIGDLELFR 361

Query: 402 TVAEKFSSTFSSDRTNNLIVRLRHNVIRTGLRNISISYSRISLADVAKKLRLDSENPVAD 461
           TVAEKFSSTFSSDRTNNLIVRLRHNVIRTGLRNISISYSRISLADVAKKLRLDSENP+AD
Sbjct: 362 TVAEKFSSTFSSDRTNNLIVRLRHNVIRTGLRNISISYSRISLADVAKKLRLDSENPIAD 421

Query: 462 AESIVSKAIRDGAIDATVDHANGWMVSKETGDIYSTNEPQIAFNSRIAFCLNMHNEAVRA 521
           AESIVSKAIRDGAIDATVDHANGWMVSKETGDIYSTNEPQIAFNSRIAFCLNMHNEAVRA
Sbjct: 422 AESIVSKAIRDGAIDATVDHANGWMVSKETGDIYSTNEPQIAFNSRIAFCLNMHNEAVRA 481

Query: 522 LRFPPNSHKEKESAEKRRERQQQEQELAKHIAEEDEDDF 561
           LRFPPNSHKEKESAEKRRERQQQEQELAKHIAEED+DDF
Sbjct: 482 LRFPPNSHKEKESAEKRRERQQQEQELAKHIAEEDDDDF 485

BLAST of Spg014773 vs. NCBI nr
Match: XP_022959338.1 (probable 26S proteasome non-ATPase regulatory subunit 3 isoform X1 [Cucurbita moschata] >XP_022959339.1 probable 26S proteasome non-ATPase regulatory subunit 3 isoform X1 [Cucurbita moschata] >XP_022959340.1 probable 26S proteasome non-ATPase regulatory subunit 3 isoform X2 [Cucurbita moschata] >XP_022959341.1 probable 26S proteasome non-ATPase regulatory subunit 3 isoform X2 [Cucurbita moschata])

HSP 1 Score: 870.2 bits (2247), Expect = 1.0e-248
Identity = 466/520 (89.62%), Postives = 483/520 (92.88%), Query Frame = 0

Query: 42  TQDAEMKELPAPVSSSSAPTSPSTLQRKKFYLHFVMLVEFLEDLVLKFVND-FFQSDLKE 101
           TQDAEMKELPAP+ SSS+ TSP           F   ++ LE LV KF++D FFQS LKE
Sbjct: 2   TQDAEMKELPAPLPSSSSSTSPQL---------FSGFIQSLECLVFKFLSDFFFQSVLKE 61

Query: 102 IVSLIETGAYAREVRRIVRAIRLTMALRRKLKASVLSSFLNFSLPPGSDVHTRLSSFIPK 161
           IVSLIETGAYAREVRR+VRAIRLTMALRRKLKASVLS FLN  LPPGS+VHTRLSSF+PK
Sbjct: 62  IVSLIETGAYAREVRRVVRAIRLTMALRRKLKASVLSQFLNLVLPPGSEVHTRLSSFLPK 121

Query: 162 EDVYEMDVDSATQALVKHPLPEIEIFCYLIVLIFLIDQKKYDEAKACASASIARLKNLNR 221
           ED +EMDVD+ATQA VKHPLPEIEIFCYLIVLIF IDQKKYD+AKACASASI RLK+LNR
Sbjct: 122 EDDFEMDVDTATQAPVKHPLPEIEIFCYLIVLIFFIDQKKYDDAKACASASITRLKSLNR 181

Query: 222 RTVDVLASRLYFYYSLSYELTGDLAEIRGNLLALHRIGTLRHDELGQETLLNLLLRNYLH 281
           RTVDVLASRLYFYYSLSYELTG+LAEIRGNLLALHRI TLRHDELGQET+LNLLLRNYLH
Sbjct: 182 RTVDVLASRLYFYYSLSYELTGELAEIRGNLLALHRIATLRHDELGQETILNLLLRNYLH 241

Query: 282 YNLYDQAEKLRSKAPRFEAHSNQQASLSALFCRYLFYLGKIRTIQLEYTDAKESLLQAAR 341
           YNLYDQAEKLRSKAPRFEAHSNQQ      FCRYLFYLGKIRTIQLEYTDAKESLLQAAR
Sbjct: 242 YNLYDQAEKLRSKAPRFEAHSNQQ------FCRYLFYLGKIRTIQLEYTDAKESLLQAAR 301

Query: 342 KAPIAALGFRVQCNKWAIIVRLLLGEIPERTVFMQKGMETALRPYFELTNAVRIGDLELF 401
           KAPIAALGFRVQCNKWAIIVRLLLGEIPERTVFMQKGMETALRPYFELTNAVRIGDLELF
Sbjct: 302 KAPIAALGFRVQCNKWAIIVRLLLGEIPERTVFMQKGMETALRPYFELTNAVRIGDLELF 361

Query: 402 RTVAEKFSSTFSSDRTNNLIVRLRHNVIRTGLRNISISYSRISLADVAKKLRLDSENPVA 461
           RTVAEKFSSTFSSDRTNNLIVRLRHNVIRTGLRNISISYSRISL DVAKKLRLDS NPVA
Sbjct: 362 RTVAEKFSSTFSSDRTNNLIVRLRHNVIRTGLRNISISYSRISLVDVAKKLRLDSPNPVA 421

Query: 462 DAESIVSKAIRDGAIDATVDHANGWMVSKETGDIYSTNEPQIAFNSRIAFCLNMHNEAVR 521
           DAESIVSKAIRDGAIDATVDHANGWMVSKETGDIYSTNEPQ+AFNSRIAFCLNMHNEAVR
Sbjct: 422 DAESIVSKAIRDGAIDATVDHANGWMVSKETGDIYSTNEPQMAFNSRIAFCLNMHNEAVR 481

Query: 522 ALRFPPNSHKEKESAEKRRERQQQEQELAKHIAEEDEDDF 561
           ALRFPPNSHKEKESAEKRRERQQQEQELAKHIAEED+DDF
Sbjct: 482 ALRFPPNSHKEKESAEKRRERQQQEQELAKHIAEEDDDDF 506

BLAST of Spg014773 vs. NCBI nr
Match: XP_022136128.1 (probable 26S proteasome non-ATPase regulatory subunit 3 [Momordica charantia] >XP_022136129.1 probable 26S proteasome non-ATPase regulatory subunit 3 [Momordica charantia] >XP_022136130.1 probable 26S proteasome non-ATPase regulatory subunit 3 [Momordica charantia])

HSP 1 Score: 863.2 bits (2229), Expect = 1.2e-246
Identity = 468/520 (90.00%), Postives = 474/520 (91.15%), Query Frame = 0

Query: 42  TQDAEMKELPAPVSSSS-APTSPSTLQRKKFYLHFVMLVEFLEDLVLKFVNDFFQSDLKE 101
           TQDAEMKELPAP+SSSS A TSPS LQ                              LKE
Sbjct: 2   TQDAEMKELPAPISSSSAASTSPSALQH-----------------------------LKE 61

Query: 102 IVSLIETGAYAREVRRIVRAIRLTMALRRKLKASVLSSFLNFSLPPGSDVHTRLSSFIPK 161
           IVSLIETGAYAREVRRIVRAIRLTMALRRKLKASVLSSFLN +L PGSDVHTRLSSF+PK
Sbjct: 62  IVSLIETGAYAREVRRIVRAIRLTMALRRKLKASVLSSFLNSALQPGSDVHTRLSSFLPK 121

Query: 162 EDVYEMDVDSATQALVKHPLPEIEIFCYLIVLIFLIDQKKYDEAKACASASIARLKNLNR 221
           ED YEMDVDSATQA  KHPLPEIEIFCYLIVLIFLIDQKKYDEAKACA+ASIARLKNLNR
Sbjct: 122 EDDYEMDVDSATQAPAKHPLPEIEIFCYLIVLIFLIDQKKYDEAKACATASIARLKNLNR 181

Query: 222 RTVDVLASRLYFYYSLSYELTGDLAEIRGNLLALHRIGTLRHDELGQETLLNLLLRNYLH 281
           RTVDVLASRLYFYYSLSYELTGDLAEIRGNLLALHRI TLRHDELGQETLLNLLLRNYLH
Sbjct: 182 RTVDVLASRLYFYYSLSYELTGDLAEIRGNLLALHRIATLRHDELGQETLLNLLLRNYLH 241

Query: 282 YNLYDQAEKLRSKAPRFEAHSNQQASLSALFCRYLFYLGKIRTIQLEYTDAKESLLQAAR 341
           YNLYDQAEKLRSKAPRFEAHSNQQ      FCRYLFYLGKIRTIQLEYTDAKESLLQAAR
Sbjct: 242 YNLYDQAEKLRSKAPRFEAHSNQQ------FCRYLFYLGKIRTIQLEYTDAKESLLQAAR 301

Query: 342 KAPIAALGFRVQCNKWAIIVRLLLGEIPERTVFMQKGMETALRPYFELTNAVRIGDLELF 401
           KAPIAALGFRVQCNKWAIIVRLLLGEIPERTVFMQKGMETALRPYFELTNAVRIGDLELF
Sbjct: 302 KAPIAALGFRVQCNKWAIIVRLLLGEIPERTVFMQKGMETALRPYFELTNAVRIGDLELF 361

Query: 402 RTVAEKFSSTFSSDRTNNLIVRLRHNVIRTGLRNISISYSRISLADVAKKLRLDSENPVA 461
           RTVAEKFSSTFSSDRTNNLIVRLRHNVIRT LRNISISYSRISLADVAKKLRLDSENPVA
Sbjct: 362 RTVAEKFSSTFSSDRTNNLIVRLRHNVIRTALRNISISYSRISLADVAKKLRLDSENPVA 421

Query: 462 DAESIVSKAIRDGAIDATVDHANGWMVSKETGDIYSTNEPQIAFNSRIAFCLNMHNEAVR 521
           DAESIVSKAIRDGAIDATVDHANGWMVSKETGDIYSTNEPQ+AFNSRIAFCLNMHNEAVR
Sbjct: 422 DAESIVSKAIRDGAIDATVDHANGWMVSKETGDIYSTNEPQMAFNSRIAFCLNMHNEAVR 481

Query: 522 ALRFPPNSHKEKESAEKRRERQQQEQELAKHIAEEDEDDF 561
           ALRFPPNSHKEKESAEKRRERQQQEQELAKHIAEED+DDF
Sbjct: 482 ALRFPPNSHKEKESAEKRRERQQQEQELAKHIAEEDDDDF 486

BLAST of Spg014773 vs. NCBI nr
Match: XP_022953127.1 (probable 26S proteasome non-ATPase regulatory subunit 3 [Cucurbita moschata] >XP_022953128.1 probable 26S proteasome non-ATPase regulatory subunit 3 [Cucurbita moschata])

HSP 1 Score: 861.3 bits (2224), Expect = 4.6e-246
Identity = 461/523 (88.15%), Postives = 476/523 (91.01%), Query Frame = 0

Query: 42  TQDAEMKELPAPVSSSSAPTSPSTLQRKKFYLHFVMLVEFLEDLVLKFVNDFFQSDLKEI 101
           TQDAEMKELP P+SSS+APTSPSTLQ                              LKEI
Sbjct: 2   TQDAEMKELPPPISSSAAPTSPSTLQH-----------------------------LKEI 61

Query: 102 VSLIETGAYAREVRRIVRAIRLTMALRRKLKASVLSSFLNFSLPPGSDVHTRLSSFIPKE 161
           VSL+ETGAYAREVRRIVRAIRLTMALR+KLKASVLS FLNF+LPPGSDVHTRLSSFIPKE
Sbjct: 62  VSLLETGAYAREVRRIVRAIRLTMALRQKLKASVLSPFLNFALPPGSDVHTRLSSFIPKE 121

Query: 162 DVYEMDVDSATQAL----VKHPLPEIEIFCYLIVLIFLIDQKKYDEAKACASASIARLKN 221
           D YEMD+DS T+AL    VKHPLPEIEIFCYL++L+FLIDQKKY EAKACASASIARLKN
Sbjct: 122 DDYEMDIDSETKALTQAPVKHPLPEIEIFCYLLLLVFLIDQKKYGEAKACASASIARLKN 181

Query: 222 LNRRTVDVLASRLYFYYSLSYELTGDLAEIRGNLLALHRIGTLRHDELGQETLLNLLLRN 281
           LNRRTVDVL+SRLYFYYSLSYELTGDLAEIRGNLLALHRI TLRHDELGQETLLNLLLRN
Sbjct: 182 LNRRTVDVLSSRLYFYYSLSYELTGDLAEIRGNLLALHRIATLRHDELGQETLLNLLLRN 241

Query: 282 YLHYNLYDQAEKLRSKAPRFEAHSNQQASLSALFCRYLFYLGKIRTIQLEYTDAKESLLQ 341
           YLHYNLYDQAEKLRSKAPRFEAHSNQQ      FCRYLFYLGKIRTIQLEYTDAKESLLQ
Sbjct: 242 YLHYNLYDQAEKLRSKAPRFEAHSNQQ------FCRYLFYLGKIRTIQLEYTDAKESLLQ 301

Query: 342 AARKAPIAALGFRVQCNKWAIIVRLLLGEIPERTVFMQKGMETALRPYFELTNAVRIGDL 401
           AARKAPIAALGFRVQCNKWAIIVRLLLGEIPERTVFMQKGMETALRPYFELTNAVRIGDL
Sbjct: 302 AARKAPIAALGFRVQCNKWAIIVRLLLGEIPERTVFMQKGMETALRPYFELTNAVRIGDL 361

Query: 402 ELFRTVAEKFSSTFSSDRTNNLIVRLRHNVIRTGLRNISISYSRISLADVAKKLRLDSEN 461
           ELFRTVA+KFSSTFSSDRTNNLIVRLRHNVIRTGLRNISISYSRISLAD+AKKLRLDSEN
Sbjct: 362 ELFRTVADKFSSTFSSDRTNNLIVRLRHNVIRTGLRNISISYSRISLADIAKKLRLDSEN 421

Query: 462 PVADAESIVSKAIRDGAIDATVDHANGWMVSKETGDIYSTNEPQIAFNSRIAFCLNMHNE 521
           P+ADAESIVSKAIRDGAIDATVDHA GWMVSKETGDIYSTNEPQIAFNSRIAFCLNMHNE
Sbjct: 422 PIADAESIVSKAIRDGAIDATVDHAKGWMVSKETGDIYSTNEPQIAFNSRIAFCLNMHNE 481

Query: 522 AVRALRFPPNSHKEKESAEKRRERQQQEQELAKHIAEEDEDDF 561
           AVRALRFPPNSHKEKESAEKRRERQQQEQELAKHIAEED+DDF
Sbjct: 482 AVRALRFPPNSHKEKESAEKRRERQQQEQELAKHIAEEDDDDF 489

BLAST of Spg014773 vs. ExPASy Swiss-Prot
Match: P93768 (Probable 26S proteasome non-ATPase regulatory subunit 3 OS=Nicotiana tabacum OX=4097 GN=21D7 PE=2 SV=1)

HSP 1 Score: 795.8 bits (2054), Expect = 3.1e-229
Identity = 432/522 (82.76%), Postives = 455/522 (87.16%), Query Frame = 0

Query: 42  TQDAEMKELPAPVSSSSAPTSPSTLQRKKFYLHFVMLVEFLEDLVLKFVNDFFQSDLKEI 101
           TQD EMKE  AP S+S + T+PS                                 LKEI
Sbjct: 2   TQDVEMKEQAAPPSNSLSSTAPSIFHH-----------------------------LKEI 61

Query: 102 VSLIETGAYAREVRRIVRAIRLTMALRRKLKASVLSSFLNFSLPPGSDVHTRLSSFIPKE 161
            SLIETGAYAREVRRI RA+RLTMALR+KLKAS LS+FLN+ L PGS+VH+RLSSF+PKE
Sbjct: 62  ASLIETGAYAREVRRISRAVRLTMALRKKLKASSLSAFLNYVLVPGSEVHSRLSSFLPKE 121

Query: 162 DVYEMDVDSAT---QALVKHPLPEIEIFCYLIVLIFLIDQKKYDEAKACASASIARLKNL 221
           D  +M+VD+AT   QA +K+PLPE+EI+CYL+VLIFLIDQKKY+EAKAC+SASIARLK +
Sbjct: 122 DEQDMEVDTATSGAQAPIKNPLPELEIYCYLLVLIFLIDQKKYNEAKACSSASIARLKTV 181

Query: 222 NRRTVDVLASRLYFYYSLSYELTGDLAEIRGNLLALHRIGTLRHDELGQETLLNLLLRNY 281
           NRRTVDVLASRLYFYYSL YELTGDLAEIRG LLALHRI TLRHDELGQETLLNLLLRNY
Sbjct: 182 NRRTVDVLASRLYFYYSLCYELTGDLAEIRGYLLALHRIATLRHDELGQETLLNLLLRNY 241

Query: 282 LHYNLYDQAEKLRSKAPRFEAHSNQQASLSALFCRYLFYLGKIRTIQLEYTDAKESLLQA 341
           LHYNLYDQAEKLRSKAPRFEAHSNQQ      F RYLFYLGKIRTIQLEYTDAKESLLQA
Sbjct: 242 LHYNLYDQAEKLRSKAPRFEAHSNQQ------FSRYLFYLGKIRTIQLEYTDAKESLLQA 301

Query: 342 ARKAPIAALGFRVQCNKWAIIVRLLLGEIPERTVFMQKGMETALRPYFELTNAVRIGDLE 401
           ARKAP AALGFRVQCNKWAIIVRLLLGEIPERTVFMQKGME ALRPYFELTNAVRIGDLE
Sbjct: 302 ARKAPQAALGFRVQCNKWAIIVRLLLGEIPERTVFMQKGMEKALRPYFELTNAVRIGDLE 361

Query: 402 LFRTVAEKFSSTFSSDRTNNLIVRLRHNVIRTGLRNISISYSRISLADVAKKLRLDSENP 461
           LFR VAEKFSSTFSSD TNNLIVRLRHNVIRTGLRNISISYSRISL DVAKKLRLDS NP
Sbjct: 362 LFRKVAEKFSSTFSSDGTNNLIVRLRHNVIRTGLRNISISYSRISLVDVAKKLRLDSPNP 421

Query: 462 VADAESIVSKAIRDGAIDATVDHANGWMVSKETGDIYSTNEPQIAFNSRIAFCLNMHNEA 521
           VADAESIVSKAIRDGAIDAT+DHANGWMVSKETGDIYSTNEPQIAFNSRIAFCLNMHNEA
Sbjct: 422 VADAESIVSKAIRDGAIDATLDHANGWMVSKETGDIYSTNEPQIAFNSRIAFCLNMHNEA 481

Query: 522 VRALRFPPNSHKEKESAEKRRERQQQEQELAKHIAEEDEDDF 561
           VRALRFPPNSHKEKESAEKRRERQQQEQELAKHIAEED+DDF
Sbjct: 482 VRALRFPPNSHKEKESAEKRRERQQQEQELAKHIAEEDDDDF 488

BLAST of Spg014773 vs. ExPASy Swiss-Prot
Match: Q06364 (Probable 26S proteasome non-ATPase regulatory subunit 3 OS=Daucus carota OX=4039 GN=21D7 PE=1 SV=2)

HSP 1 Score: 791.6 bits (2043), Expect = 5.9e-228
Identity = 422/523 (80.69%), Postives = 458/523 (87.57%), Query Frame = 0

Query: 42  TQDAEMKELPAPVSSSS-APTSPSTLQRKKFYLHFVMLVEFLEDLVLKFVNDFFQSDLKE 101
           TQD EMKE+PAP  S+S    +PSTLQ                              LKE
Sbjct: 2   TQDVEMKEVPAPAPSNSVTAATPSTLQH-----------------------------LKE 61

Query: 102 IVSLIETGAYAREVRRIVRAIRLTMALRRKLKASVLSSFLNFSLPPGSDVHTRLSSFIPK 161
           I SLIE+GAYAREVRRI+RA+RLT+ALR+KL ASV+++FLNFSL PGS+VH RL+S++PK
Sbjct: 62  IASLIESGAYAREVRRILRAVRLTIALRKKLNASVVNAFLNFSLVPGSEVHARLASYLPK 121

Query: 162 EDVYEMDVD---SATQALVKHPLPEIEIFCYLIVLIFLIDQKKYDEAKACASASIARLKN 221
           ED ++M+VD   SAT  L KH LPE+EI+CYL+VLIFLIDQKKY EAKAC+SASIAR+KN
Sbjct: 122 EDEHDMEVDTAMSATTTLAKHSLPELEIYCYLLVLIFLIDQKKYSEAKACSSASIARVKN 181

Query: 222 LNRRTVDVLASRLYFYYSLSYELTGDLAEIRGNLLALHRIGTLRHDELGQETLLNLLLRN 281
           LNRRTV+VLASRLYFYYSLSYELTGDLAEIRGNLLALHRI TLRHDELGQETLLNLLLRN
Sbjct: 182 LNRRTVEVLASRLYFYYSLSYELTGDLAEIRGNLLALHRIATLRHDELGQETLLNLLLRN 241

Query: 282 YLHYNLYDQAEKLRSKAPRFEAHSNQQASLSALFCRYLFYLGKIRTIQLEYTDAKESLLQ 341
           YLHYNLYDQAEKLRSKAPRFEAHSNQQ      FCRYLFYLGKIRTIQLEYTDAKES LQ
Sbjct: 242 YLHYNLYDQAEKLRSKAPRFEAHSNQQ------FCRYLFYLGKIRTIQLEYTDAKESXLQ 301

Query: 342 AARKAPIAALGFRVQCNKWAIIVRLLLGEIPERTVFMQKGMETALRPYFELTNAVRIGDL 401
           AARKAP+AALGFRVQCNKWA+IVRLLLGEIPERTVFMQKGME ALRPYF LTNAVRIGDL
Sbjct: 302 AARKAPVAALGFRVQCNKWAVIVRLLLGEIPERTVFMQKGMEKALRPYFXLTNAVRIGDL 361

Query: 402 ELFRTVAEKFSSTFSSDRTNNLIVRLRHNVIRTGLRNISISYSRISLADVAKKLRLDSEN 461
           ELFR VA+KF+STF++DRT+NLIVRLRHNVIRTGLRNISISYSRISL DVA+KLRLDS N
Sbjct: 362 ELFRXVADKFASTFTADRTHNLIVRLRHNVIRTGLRNISISYSRISLVDVARKLRLDSPN 421

Query: 462 PVADAESIVSKAIRDGAIDATVDHANGWMVSKETGDIYSTNEPQIAFNSRIAFCLNMHNE 521
           PVADAESIVSKAIRDGAIDAT+DHANGWMVSKETGDIYSTNEPQ AFNSRIAFCLNMHNE
Sbjct: 422 PVADAESIVSKAIRDGAIDATIDHANGWMVSKETGDIYSTNEPQAAFNSRIAFCLNMHNE 481

Query: 522 AVRALRFPPNSHKEKESAEKRRERQQQEQELAKHIAEEDEDDF 561
           AVRALRFP NSHK+KESAEKRRERQQQEQELAKHIAEED+D+F
Sbjct: 482 AVRALRFPANSHKDKESAEKRRERQQQEQELAKHIAEEDDDEF 489

BLAST of Spg014773 vs. ExPASy Swiss-Prot
Match: Q9LNU4 (26S proteasome non-ATPase regulatory subunit 3 homolog A OS=Arabidopsis thaliana OX=3702 GN=RPN3A PE=1 SV=3)

HSP 1 Score: 741.9 bits (1914), Expect = 5.4e-213
Identity = 401/523 (76.67%), Postives = 442/523 (84.51%), Query Frame = 0

Query: 42  TQDAEMKELPAPVSSSSAPTSPSTLQRKKFYLHFVMLVEFLEDLVLKFVNDFFQSDLKEI 101
           TQD EMK+   P S S   +S ST+Q                             +LKEI
Sbjct: 2   TQDVEMKDNNTP-SQSIISSSTSTMQ-----------------------------NLKEI 61

Query: 102 VSLIETGAYAREVRRIVRAIRLTMALRRKLKASVLSSFLNFSLPPGSDVHTRLSSFIPKE 161
            +LI+TG+Y +EVRRI RA+RLT+ LR+KL  SVLSSFL+F+L PGS+ H+RLSSF+PK 
Sbjct: 62  AALIDTGSYTKEVRRIARAVRLTIGLRQKLTGSVLSSFLDFALVPGSEAHSRLSSFVPKG 121

Query: 162 DVYEMDVD---SATQALVKHPLP-EIEIFCYLIVLIFLIDQKKYDEAKACASASIARLKN 221
           D ++M+VD   SATQA     LP E+EI+CY IVL+FLIDQKKY+EAKAC+SASIARLKN
Sbjct: 122 DEHDMEVDTASSATQAAPSKHLPAELEIYCYFIVLLFLIDQKKYNEAKACSSASIARLKN 181

Query: 222 LNRRTVDVLASRLYFYYSLSYELTGDLAEIRGNLLALHRIGTLRHDELGQETLLNLLLRN 281
           +NRRT+DV+ASRLYFYYSLSYE TGDLAEIRG LLALH   TLRHDELGQETLLNLLLRN
Sbjct: 182 VNRRTIDVIASRLYFYYSLSYEQTGDLAEIRGTLLALHHSATLRHDELGQETLLNLLLRN 241

Query: 282 YLHYNLYDQAEKLRSKAPRFEAHSNQQASLSALFCRYLFYLGKIRTIQLEYTDAKESLLQ 341
           YLHYNLYDQAEKLRSKAPRFEAHSNQQ      FCRYLFYLGKIRTIQLEYTDAKESLLQ
Sbjct: 242 YLHYNLYDQAEKLRSKAPRFEAHSNQQ------FCRYLFYLGKIRTIQLEYTDAKESLLQ 301

Query: 342 AARKAPIAALGFRVQCNKWAIIVRLLLGEIPERTVFMQKGMETALRPYFELTNAVRIGDL 401
           AARKAPIAALGFR+QCNKWAI+VRLLLGEIPER++F QKGME ALRPYFELTNAVRIGDL
Sbjct: 302 AARKAPIAALGFRIQCNKWAILVRLLLGEIPERSIFTQKGMEKALRPYFELTNAVRIGDL 361

Query: 402 ELFRTVAEKFSSTFSSDRTNNLIVRLRHNVIRTGLRNISISYSRISLADVAKKLRLDSEN 461
           ELFRTV EKF  TF+ DRT+NLIVRLRHNVIRTGLRNISISYSRISL DVAKKLRL+SEN
Sbjct: 362 ELFRTVQEKFLDTFAQDRTHNLIVRLRHNVIRTGLRNISISYSRISLPDVAKKLRLNSEN 421

Query: 462 PVADAESIVSKAIRDGAIDATVDHANGWMVSKETGDIYSTNEPQIAFNSRIAFCLNMHNE 521
           PVADAESIV+KAIRDGAIDAT+DH NG MVSKETGDIYSTNEPQ AFNSRIAFCLNMHNE
Sbjct: 422 PVADAESIVAKAIRDGAIDATIDHKNGCMVSKETGDIYSTNEPQTAFNSRIAFCLNMHNE 481

Query: 522 AVRALRFPPNSHKEKESAEKRRERQQQEQELAKHIAEEDEDDF 561
           AVRALRFPPN+HKEKES EKRRER+QQE+ELAKH+AEED+DDF
Sbjct: 482 AVRALRFPPNTHKEKESDEKRRERKQQEEELAKHMAEEDDDDF 488

BLAST of Spg014773 vs. ExPASy Swiss-Prot
Match: Q9LQR8 (26S proteasome non-ATPase regulatory subunit 3 homolog B OS=Arabidopsis thaliana OX=3702 GN=RPN3B PE=1 SV=2)

HSP 1 Score: 723.8 bits (1867), Expect = 1.5e-207
Identity = 389/523 (74.38%), Postives = 437/523 (83.56%), Query Frame = 0

Query: 42  TQDAEMKELPAPVSS-SSAPTSPSTLQRKKFYLHFVMLVEFLEDLVLKFVNDFFQSDLKE 101
           TQD EMK+   P  S  SAPT  STLQ                             +LKE
Sbjct: 2   TQDVEMKDNQTPTQSVVSAPT--STLQ-----------------------------NLKE 61

Query: 102 IVSLIETGAYAREVRRIVRAIRLTMALRRKLKASVLSSFLNFSLPPGSDVHTRLSSFIPK 161
           I +LI+TG+Y +EVRRI RA+RLT+ LRRKL  SV+SSFL+F+L PGS+ HTRLSSF+PK
Sbjct: 62  IAALIDTGSYTKEVRRIARAVRLTVGLRRKLTGSVISSFLDFALVPGSEAHTRLSSFVPK 121

Query: 162 EDVYEMDVDSA---TQALVKHPLPEIEIFCYLIVLIFLIDQKKYDEAKACASASIARLKN 221
            D ++M+VD+A   +QA  KH   E+EI+CY IVL+FLIDQKKY+EAK C++ASIARLK+
Sbjct: 122 SDEHDMEVDTASSNSQAPPKHLPAELEIYCYFIVLLFLIDQKKYNEAKVCSTASIARLKS 181

Query: 222 LNRRTVDVLASRLYFYYSLSYELTGDLAEIRGNLLALHRIGTLRHDELGQETLLNLLLRN 281
           +NRRTVDV+AS+LYFYYSLSYELT DLAEIR  LLALH   TLRHDELGQETLLNLLLRN
Sbjct: 182 VNRRTVDVIASKLYFYYSLSYELTNDLAEIRSTLLALHHSATLRHDELGQETLLNLLLRN 241

Query: 282 YLHYNLYDQAEKLRSKAPRFEAHSNQQASLSALFCRYLFYLGKIRTIQLEYTDAKESLLQ 341
           YLHYNLYDQAEKLRSKAPRFEAHSNQQ      FCRYLFYLGKIRTIQLEYTDAKESLLQ
Sbjct: 242 YLHYNLYDQAEKLRSKAPRFEAHSNQQ------FCRYLFYLGKIRTIQLEYTDAKESLLQ 301

Query: 342 AARKAPIAALGFRVQCNKWAIIVRLLLGEIPERTVFMQKGMETALRPYFELTNAVRIGDL 401
           AARKAP+A+LGFR+QCNKWAIIVRLLLGEIPER++F QKGME  LRPYFELTNAVRIGDL
Sbjct: 302 AARKAPVASLGFRIQCNKWAIIVRLLLGEIPERSIFTQKGMEKTLRPYFELTNAVRIGDL 361

Query: 402 ELFRTVAEKFSSTFSSDRTNNLIVRLRHNVIRTGLRNISISYSRISLADVAKKLRLDSEN 461
           ELF  + EKF+ TF+ DRT+NLIVRLRHNVIRTGLRNISISYSRISL DVA+KLRL+S N
Sbjct: 362 ELFGKIQEKFAKTFAEDRTHNLIVRLRHNVIRTGLRNISISYSRISLQDVAQKLRLNSAN 421

Query: 462 PVADAESIVSKAIRDGAIDATVDHANGWMVSKETGDIYSTNEPQIAFNSRIAFCLNMHNE 521
           PVADAESIV+KAIRDGAIDAT+DH NG MVSKETGDIYSTNEPQ AFNSRIAFCLNMHNE
Sbjct: 422 PVADAESIVAKAIRDGAIDATIDHKNGCMVSKETGDIYSTNEPQTAFNSRIAFCLNMHNE 481

Query: 522 AVRALRFPPNSHKEKESAEKRRERQQQEQELAKHIAEEDEDDF 561
           AVRALRFPPN+H+EKES EKRRE +QQE+ELAK++AEED+DDF
Sbjct: 482 AVRALRFPPNTHREKESEEKRREMKQQEEELAKYMAEEDDDDF 487

BLAST of Spg014773 vs. ExPASy Swiss-Prot
Match: Q2KJ46 (26S proteasome non-ATPase regulatory subunit 3 OS=Bos taurus OX=9913 GN=PSMD3 PE=2 SV=1)

HSP 1 Score: 372.1 bits (954), Expect = 1.1e-101
Identity = 229/523 (43.79%), Postives = 326/523 (62.33%), Query Frame = 0

Query: 43  QDAEMKELPAPVSSSSAPTSPSTLQRKKFYLHFVMLVEFLEDLVLKFVNDFFQSDLKEIV 102
           QD EMKE  A    S+  T+  T      +    +    LE             D+KE V
Sbjct: 33  QDVEMKEEAAAGGGSTGETAGKTAAAAAEHSQRELDTVTLE-------------DIKEHV 92

Query: 103 SLIETGAYAREVRRIVRAIRLTMALRRKLKASVLSSFLNFSLPPGSDVHTRLSSFI--PK 162
             +E     +E R ++RA+R+  +  R+L   VL   ++      +     L  F+  P 
Sbjct: 93  KQLEKAVSGKEPRFVLRALRMLPSTSRRLNHYVLYKAVHGFFTSNNATRDFLLPFLEEPM 152

Query: 163 EDVYEMDVDSATQALVKHP-LPEIEIFCYLIVLIFLIDQKKYDEAKACASASIARLKNLN 222
           +   ++     T      P LPE+E +  L+++IFL++ K+Y EA+  +   + ++   N
Sbjct: 153 DTEADLQFRPRTGKAASAPLLPEVEAYLQLLMVIFLMNSKRYKEAQKISDDLMQKISTQN 212

Query: 223 RRTVDVLASRLYFYYSLSYELTGDLAEIRGNLLALHRIGTLRHDELGQETLLNLLLRNYL 282
           RR +D++A++ Y+Y++  YE    L  +R  L A  R  TLRHD  GQ TLLNLLLRNYL
Sbjct: 213 RRALDLVAAKCYYYHARVYEFLDKLDVVRSFLHARLRTATLRHDTDGQATLLNLLLRNYL 272

Query: 283 HYNLYDQAEKLRSKAPRFEAHSNQQASLSALFCRYLFYLGKIRTIQLEYTDAKESLLQAA 342
           HY+LYDQAEKL SK+   E  +N +      + RYL+Y G+I+ IQLEY++A+ ++  A 
Sbjct: 273 HYSLYDQAEKLVSKSVFPEQANNNE------WARYLYYTGRIKAIQLEYSEARRTMTNAL 332

Query: 343 RKAP-IAALGFRVQCNKWAIIVRLLLGEIPERTVFMQKGMETALRPYFELTNAVRIGDLE 402
           RKAP   A+GF+   +K  I+V LLLGEIP+R  F Q  ++ +L PYF LT AVR G+L 
Sbjct: 333 RKAPQHTAVGFKQTVHKLLIVVELLLGEIPDRLQFRQPSLKRSLMPYFLLTQAVRTGNLA 392

Query: 403 LFRTVAEKFSSTFSSDRTNNLIVRLRHNVIRTGLRNISISYSRISLADVAKKLRLDSENP 462
            F  V ++F   F +D T  LI+RLRHNVI+TG+R IS+SYSRISLAD+A+KL+LDS   
Sbjct: 393 KFNQVLDQFGEKFQADGTYTLIIRLRHNVIKTGVRMISLSYSRISLADIAQKLQLDSPE- 452

Query: 463 VADAESIVSKAIRDGAIDATVDHANGWMVSKETGDIYSTNEPQIAFNSRIAFCLNMHNEA 522
             DAE IV+KAIRDG I+A+++H  G++ SKE  DIYST EPQ+AF+ RI+FCL++HN +
Sbjct: 453 --DAEFIVAKAIRDGVIEASINHEKGYVQSKEMIDIYSTREPQLAFHQRISFCLDIHNMS 512

Query: 523 VRALRFPPNSH-KEKESAEKRRERQQQEQELAKHIAEEDEDDF 561
           V+A+RFPP S+ K+ ESAE+RRER+QQ+ E AK +AE+D+D F
Sbjct: 513 VKAMRFPPKSYNKDLESAEERREREQQDLEFAKEMAEDDDDSF 533

BLAST of Spg014773 vs. ExPASy TrEMBL
Match: A0A6J1H5N0 (probable 26S proteasome non-ATPase regulatory subunit 3 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111460341 PE=3 SV=1)

HSP 1 Score: 870.2 bits (2247), Expect = 4.8e-249
Identity = 466/520 (89.62%), Postives = 483/520 (92.88%), Query Frame = 0

Query: 42  TQDAEMKELPAPVSSSSAPTSPSTLQRKKFYLHFVMLVEFLEDLVLKFVND-FFQSDLKE 101
           TQDAEMKELPAP+ SSS+ TSP           F   ++ LE LV KF++D FFQS LKE
Sbjct: 2   TQDAEMKELPAPLPSSSSSTSPQL---------FSGFIQSLECLVFKFLSDFFFQSVLKE 61

Query: 102 IVSLIETGAYAREVRRIVRAIRLTMALRRKLKASVLSSFLNFSLPPGSDVHTRLSSFIPK 161
           IVSLIETGAYAREVRR+VRAIRLTMALRRKLKASVLS FLN  LPPGS+VHTRLSSF+PK
Sbjct: 62  IVSLIETGAYAREVRRVVRAIRLTMALRRKLKASVLSQFLNLVLPPGSEVHTRLSSFLPK 121

Query: 162 EDVYEMDVDSATQALVKHPLPEIEIFCYLIVLIFLIDQKKYDEAKACASASIARLKNLNR 221
           ED +EMDVD+ATQA VKHPLPEIEIFCYLIVLIF IDQKKYD+AKACASASI RLK+LNR
Sbjct: 122 EDDFEMDVDTATQAPVKHPLPEIEIFCYLIVLIFFIDQKKYDDAKACASASITRLKSLNR 181

Query: 222 RTVDVLASRLYFYYSLSYELTGDLAEIRGNLLALHRIGTLRHDELGQETLLNLLLRNYLH 281
           RTVDVLASRLYFYYSLSYELTG+LAEIRGNLLALHRI TLRHDELGQET+LNLLLRNYLH
Sbjct: 182 RTVDVLASRLYFYYSLSYELTGELAEIRGNLLALHRIATLRHDELGQETILNLLLRNYLH 241

Query: 282 YNLYDQAEKLRSKAPRFEAHSNQQASLSALFCRYLFYLGKIRTIQLEYTDAKESLLQAAR 341
           YNLYDQAEKLRSKAPRFEAHSNQQ      FCRYLFYLGKIRTIQLEYTDAKESLLQAAR
Sbjct: 242 YNLYDQAEKLRSKAPRFEAHSNQQ------FCRYLFYLGKIRTIQLEYTDAKESLLQAAR 301

Query: 342 KAPIAALGFRVQCNKWAIIVRLLLGEIPERTVFMQKGMETALRPYFELTNAVRIGDLELF 401
           KAPIAALGFRVQCNKWAIIVRLLLGEIPERTVFMQKGMETALRPYFELTNAVRIGDLELF
Sbjct: 302 KAPIAALGFRVQCNKWAIIVRLLLGEIPERTVFMQKGMETALRPYFELTNAVRIGDLELF 361

Query: 402 RTVAEKFSSTFSSDRTNNLIVRLRHNVIRTGLRNISISYSRISLADVAKKLRLDSENPVA 461
           RTVAEKFSSTFSSDRTNNLIVRLRHNVIRTGLRNISISYSRISL DVAKKLRLDS NPVA
Sbjct: 362 RTVAEKFSSTFSSDRTNNLIVRLRHNVIRTGLRNISISYSRISLVDVAKKLRLDSPNPVA 421

Query: 462 DAESIVSKAIRDGAIDATVDHANGWMVSKETGDIYSTNEPQIAFNSRIAFCLNMHNEAVR 521
           DAESIVSKAIRDGAIDATVDHANGWMVSKETGDIYSTNEPQ+AFNSRIAFCLNMHNEAVR
Sbjct: 422 DAESIVSKAIRDGAIDATVDHANGWMVSKETGDIYSTNEPQMAFNSRIAFCLNMHNEAVR 481

Query: 522 ALRFPPNSHKEKESAEKRRERQQQEQELAKHIAEEDEDDF 561
           ALRFPPNSHKEKESAEKRRERQQQEQELAKHIAEED+DDF
Sbjct: 482 ALRFPPNSHKEKESAEKRRERQQQEQELAKHIAEEDDDDF 506

BLAST of Spg014773 vs. ExPASy TrEMBL
Match: A0A6J1C6S2 (probable 26S proteasome non-ATPase regulatory subunit 3 OS=Momordica charantia OX=3673 GN=LOC111007897 PE=3 SV=1)

HSP 1 Score: 863.2 bits (2229), Expect = 5.9e-247
Identity = 468/520 (90.00%), Postives = 474/520 (91.15%), Query Frame = 0

Query: 42  TQDAEMKELPAPVSSSS-APTSPSTLQRKKFYLHFVMLVEFLEDLVLKFVNDFFQSDLKE 101
           TQDAEMKELPAP+SSSS A TSPS LQ                              LKE
Sbjct: 2   TQDAEMKELPAPISSSSAASTSPSALQH-----------------------------LKE 61

Query: 102 IVSLIETGAYAREVRRIVRAIRLTMALRRKLKASVLSSFLNFSLPPGSDVHTRLSSFIPK 161
           IVSLIETGAYAREVRRIVRAIRLTMALRRKLKASVLSSFLN +L PGSDVHTRLSSF+PK
Sbjct: 62  IVSLIETGAYAREVRRIVRAIRLTMALRRKLKASVLSSFLNSALQPGSDVHTRLSSFLPK 121

Query: 162 EDVYEMDVDSATQALVKHPLPEIEIFCYLIVLIFLIDQKKYDEAKACASASIARLKNLNR 221
           ED YEMDVDSATQA  KHPLPEIEIFCYLIVLIFLIDQKKYDEAKACA+ASIARLKNLNR
Sbjct: 122 EDDYEMDVDSATQAPAKHPLPEIEIFCYLIVLIFLIDQKKYDEAKACATASIARLKNLNR 181

Query: 222 RTVDVLASRLYFYYSLSYELTGDLAEIRGNLLALHRIGTLRHDELGQETLLNLLLRNYLH 281
           RTVDVLASRLYFYYSLSYELTGDLAEIRGNLLALHRI TLRHDELGQETLLNLLLRNYLH
Sbjct: 182 RTVDVLASRLYFYYSLSYELTGDLAEIRGNLLALHRIATLRHDELGQETLLNLLLRNYLH 241

Query: 282 YNLYDQAEKLRSKAPRFEAHSNQQASLSALFCRYLFYLGKIRTIQLEYTDAKESLLQAAR 341
           YNLYDQAEKLRSKAPRFEAHSNQQ      FCRYLFYLGKIRTIQLEYTDAKESLLQAAR
Sbjct: 242 YNLYDQAEKLRSKAPRFEAHSNQQ------FCRYLFYLGKIRTIQLEYTDAKESLLQAAR 301

Query: 342 KAPIAALGFRVQCNKWAIIVRLLLGEIPERTVFMQKGMETALRPYFELTNAVRIGDLELF 401
           KAPIAALGFRVQCNKWAIIVRLLLGEIPERTVFMQKGMETALRPYFELTNAVRIGDLELF
Sbjct: 302 KAPIAALGFRVQCNKWAIIVRLLLGEIPERTVFMQKGMETALRPYFELTNAVRIGDLELF 361

Query: 402 RTVAEKFSSTFSSDRTNNLIVRLRHNVIRTGLRNISISYSRISLADVAKKLRLDSENPVA 461
           RTVAEKFSSTFSSDRTNNLIVRLRHNVIRT LRNISISYSRISLADVAKKLRLDSENPVA
Sbjct: 362 RTVAEKFSSTFSSDRTNNLIVRLRHNVIRTALRNISISYSRISLADVAKKLRLDSENPVA 421

Query: 462 DAESIVSKAIRDGAIDATVDHANGWMVSKETGDIYSTNEPQIAFNSRIAFCLNMHNEAVR 521
           DAESIVSKAIRDGAIDATVDHANGWMVSKETGDIYSTNEPQ+AFNSRIAFCLNMHNEAVR
Sbjct: 422 DAESIVSKAIRDGAIDATVDHANGWMVSKETGDIYSTNEPQMAFNSRIAFCLNMHNEAVR 481

Query: 522 ALRFPPNSHKEKESAEKRRERQQQEQELAKHIAEEDEDDF 561
           ALRFPPNSHKEKESAEKRRERQQQEQELAKHIAEED+DDF
Sbjct: 482 ALRFPPNSHKEKESAEKRRERQQQEQELAKHIAEEDDDDF 486

BLAST of Spg014773 vs. ExPASy TrEMBL
Match: A0A6J1GMC8 (probable 26S proteasome non-ATPase regulatory subunit 3 OS=Cucurbita moschata OX=3662 GN=LOC111455623 PE=3 SV=1)

HSP 1 Score: 861.3 bits (2224), Expect = 2.2e-246
Identity = 461/523 (88.15%), Postives = 476/523 (91.01%), Query Frame = 0

Query: 42  TQDAEMKELPAPVSSSSAPTSPSTLQRKKFYLHFVMLVEFLEDLVLKFVNDFFQSDLKEI 101
           TQDAEMKELP P+SSS+APTSPSTLQ                              LKEI
Sbjct: 2   TQDAEMKELPPPISSSAAPTSPSTLQH-----------------------------LKEI 61

Query: 102 VSLIETGAYAREVRRIVRAIRLTMALRRKLKASVLSSFLNFSLPPGSDVHTRLSSFIPKE 161
           VSL+ETGAYAREVRRIVRAIRLTMALR+KLKASVLS FLNF+LPPGSDVHTRLSSFIPKE
Sbjct: 62  VSLLETGAYAREVRRIVRAIRLTMALRQKLKASVLSPFLNFALPPGSDVHTRLSSFIPKE 121

Query: 162 DVYEMDVDSATQAL----VKHPLPEIEIFCYLIVLIFLIDQKKYDEAKACASASIARLKN 221
           D YEMD+DS T+AL    VKHPLPEIEIFCYL++L+FLIDQKKY EAKACASASIARLKN
Sbjct: 122 DDYEMDIDSETKALTQAPVKHPLPEIEIFCYLLLLVFLIDQKKYGEAKACASASIARLKN 181

Query: 222 LNRRTVDVLASRLYFYYSLSYELTGDLAEIRGNLLALHRIGTLRHDELGQETLLNLLLRN 281
           LNRRTVDVL+SRLYFYYSLSYELTGDLAEIRGNLLALHRI TLRHDELGQETLLNLLLRN
Sbjct: 182 LNRRTVDVLSSRLYFYYSLSYELTGDLAEIRGNLLALHRIATLRHDELGQETLLNLLLRN 241

Query: 282 YLHYNLYDQAEKLRSKAPRFEAHSNQQASLSALFCRYLFYLGKIRTIQLEYTDAKESLLQ 341
           YLHYNLYDQAEKLRSKAPRFEAHSNQQ      FCRYLFYLGKIRTIQLEYTDAKESLLQ
Sbjct: 242 YLHYNLYDQAEKLRSKAPRFEAHSNQQ------FCRYLFYLGKIRTIQLEYTDAKESLLQ 301

Query: 342 AARKAPIAALGFRVQCNKWAIIVRLLLGEIPERTVFMQKGMETALRPYFELTNAVRIGDL 401
           AARKAPIAALGFRVQCNKWAIIVRLLLGEIPERTVFMQKGMETALRPYFELTNAVRIGDL
Sbjct: 302 AARKAPIAALGFRVQCNKWAIIVRLLLGEIPERTVFMQKGMETALRPYFELTNAVRIGDL 361

Query: 402 ELFRTVAEKFSSTFSSDRTNNLIVRLRHNVIRTGLRNISISYSRISLADVAKKLRLDSEN 461
           ELFRTVA+KFSSTFSSDRTNNLIVRLRHNVIRTGLRNISISYSRISLAD+AKKLRLDSEN
Sbjct: 362 ELFRTVADKFSSTFSSDRTNNLIVRLRHNVIRTGLRNISISYSRISLADIAKKLRLDSEN 421

Query: 462 PVADAESIVSKAIRDGAIDATVDHANGWMVSKETGDIYSTNEPQIAFNSRIAFCLNMHNE 521
           P+ADAESIVSKAIRDGAIDATVDHA GWMVSKETGDIYSTNEPQIAFNSRIAFCLNMHNE
Sbjct: 422 PIADAESIVSKAIRDGAIDATVDHAKGWMVSKETGDIYSTNEPQIAFNSRIAFCLNMHNE 481

Query: 522 AVRALRFPPNSHKEKESAEKRRERQQQEQELAKHIAEEDEDDF 561
           AVRALRFPPNSHKEKESAEKRRERQQQEQELAKHIAEED+DDF
Sbjct: 482 AVRALRFPPNSHKEKESAEKRRERQQQEQELAKHIAEEDDDDF 489

BLAST of Spg014773 vs. ExPASy TrEMBL
Match: A0A6J1HXW8 (probable 26S proteasome non-ATPase regulatory subunit 3 OS=Cucurbita maxima OX=3661 GN=LOC111468464 PE=3 SV=1)

HSP 1 Score: 856.7 bits (2212), Expect = 5.5e-245
Identity = 461/523 (88.15%), Postives = 475/523 (90.82%), Query Frame = 0

Query: 42  TQDAEMKELPAPVSSSSAPTSPSTLQRKKFYLHFVMLVEFLEDLVLKFVNDFFQSDLKEI 101
           TQDAEMKELP P+SSS+A TSPSTLQ                              LKEI
Sbjct: 2   TQDAEMKELPPPISSSAALTSPSTLQH-----------------------------LKEI 61

Query: 102 VSLIETGAYAREVRRIVRAIRLTMALRRKLKASVLSSFLNFSLPPGSDVHTRLSSFIPKE 161
           VSL+ETGAYAREVRRIVRAIRLTMALR+KLKASVLS FLNF+LPPGSDVHTRLSSFIPKE
Sbjct: 62  VSLLETGAYAREVRRIVRAIRLTMALRQKLKASVLSPFLNFALPPGSDVHTRLSSFIPKE 121

Query: 162 DVYEMDVDSATQAL----VKHPLPEIEIFCYLIVLIFLIDQKKYDEAKACASASIARLKN 221
           D YEMDVDS T+AL    VKHPLPEIEIFCYL++L+FLIDQKKY EAKACASASIARLKN
Sbjct: 122 DDYEMDVDSETKALTQAPVKHPLPEIEIFCYLLLLVFLIDQKKYGEAKACASASIARLKN 181

Query: 222 LNRRTVDVLASRLYFYYSLSYELTGDLAEIRGNLLALHRIGTLRHDELGQETLLNLLLRN 281
           LNRRTVDVL+SRLYFYYSLSYELTGDLAEIRGNLLALHRI TLRHDELGQETLLNLLLRN
Sbjct: 182 LNRRTVDVLSSRLYFYYSLSYELTGDLAEIRGNLLALHRIATLRHDELGQETLLNLLLRN 241

Query: 282 YLHYNLYDQAEKLRSKAPRFEAHSNQQASLSALFCRYLFYLGKIRTIQLEYTDAKESLLQ 341
           YL+YNLYDQAEKLRSKAPRFEAHSNQQ      FCRYLFYLGKIRTIQLEYTDAKESLLQ
Sbjct: 242 YLYYNLYDQAEKLRSKAPRFEAHSNQQ------FCRYLFYLGKIRTIQLEYTDAKESLLQ 301

Query: 342 AARKAPIAALGFRVQCNKWAIIVRLLLGEIPERTVFMQKGMETALRPYFELTNAVRIGDL 401
           AARKAPIAALGFRVQCNKWAIIVRLLLGEIPERTVFMQKGMETALRPYFELTNAVRIGDL
Sbjct: 302 AARKAPIAALGFRVQCNKWAIIVRLLLGEIPERTVFMQKGMETALRPYFELTNAVRIGDL 361

Query: 402 ELFRTVAEKFSSTFSSDRTNNLIVRLRHNVIRTGLRNISISYSRISLADVAKKLRLDSEN 461
           ELFRTVAEKFSSTFSSDRTNNLIVRLRHNVIRTGLRNISISYSRISLAD+AKKLRLDSEN
Sbjct: 362 ELFRTVAEKFSSTFSSDRTNNLIVRLRHNVIRTGLRNISISYSRISLADIAKKLRLDSEN 421

Query: 462 PVADAESIVSKAIRDGAIDATVDHANGWMVSKETGDIYSTNEPQIAFNSRIAFCLNMHNE 521
           P+ADAESIVSKAIRDGAIDATVDHA GWMVSKETGDIYSTNEPQIAFNSRIAFCLNMHNE
Sbjct: 422 PIADAESIVSKAIRDGAIDATVDHAKGWMVSKETGDIYSTNEPQIAFNSRIAFCLNMHNE 481

Query: 522 AVRALRFPPNSHKEKESAEKRRERQQQEQELAKHIAEEDEDDF 561
           AVRALRFPPNSHKEKESAEKRRERQQQEQELAKHIAEED+DDF
Sbjct: 482 AVRALRFPPNSHKEKESAEKRRERQQQEQELAKHIAEEDDDDF 489

BLAST of Spg014773 vs. ExPASy TrEMBL
Match: A0A6J1DBS3 (probable 26S proteasome non-ATPase regulatory subunit 3 OS=Momordica charantia OX=3673 GN=LOC111019235 PE=3 SV=1)

HSP 1 Score: 856.7 bits (2212), Expect = 5.5e-245
Identity = 461/519 (88.82%), Postives = 470/519 (90.56%), Query Frame = 0

Query: 42  TQDAEMKELPAPVSSSSAPTSPSTLQRKKFYLHFVMLVEFLEDLVLKFVNDFFQSDLKEI 101
           TQDAEM ELPAP+SSSS+ TSPSTLQ                              LKEI
Sbjct: 2   TQDAEMNELPAPLSSSSSSTSPSTLQH-----------------------------LKEI 61

Query: 102 VSLIETGAYAREVRRIVRAIRLTMALRRKLKASVLSSFLNFSLPPGSDVHTRLSSFIPKE 161
           V L+ETGAYAREVRRIVRAIRLTMALRRKLKASVLS FLN  LPPGS+VH+RLSSF+PK+
Sbjct: 62  VLLLETGAYAREVRRIVRAIRLTMALRRKLKASVLSQFLNLVLPPGSEVHSRLSSFLPKD 121

Query: 162 DVYEMDVDSATQALVKHPLPEIEIFCYLIVLIFLIDQKKYDEAKACASASIARLKNLNRR 221
           D YEMDVDSATQA  KHPLPEIEIFCYLIVLIFLIDQKKYDEAKACASASIARLKNLNRR
Sbjct: 122 DGYEMDVDSATQAPAKHPLPEIEIFCYLIVLIFLIDQKKYDEAKACASASIARLKNLNRR 181

Query: 222 TVDVLASRLYFYYSLSYELTGDLAEIRGNLLALHRIGTLRHDELGQETLLNLLLRNYLHY 281
           TVDVLASRLYFYYSLSYELTGDLAEIRGNLLALHRI TLRHDELGQETLLNLLLRNYLHY
Sbjct: 182 TVDVLASRLYFYYSLSYELTGDLAEIRGNLLALHRIATLRHDELGQETLLNLLLRNYLHY 241

Query: 282 NLYDQAEKLRSKAPRFEAHSNQQASLSALFCRYLFYLGKIRTIQLEYTDAKESLLQAARK 341
           NLYDQAEKLRSKAPRFEAHSNQQ      FCRYLFYLGKIRTIQLEYTDAKESLLQAARK
Sbjct: 242 NLYDQAEKLRSKAPRFEAHSNQQ------FCRYLFYLGKIRTIQLEYTDAKESLLQAARK 301

Query: 342 APIAALGFRVQCNKWAIIVRLLLGEIPERTVFMQKGMETALRPYFELTNAVRIGDLELFR 401
           APIAALGFRVQCNKWAIIVRLLLGEIPERTVFMQKGMETALRPYFELTNAVRIGDLELFR
Sbjct: 302 APIAALGFRVQCNKWAIIVRLLLGEIPERTVFMQKGMETALRPYFELTNAVRIGDLELFR 361

Query: 402 TVAEKFSSTFSSDRTNNLIVRLRHNVIRTGLRNISISYSRISLADVAKKLRLDSENPVAD 461
           TVAEKFSSTFS+DRTNNLIVRLRHNVIRTGLRNISISYSRISLADVAKKLRLD  NPVAD
Sbjct: 362 TVAEKFSSTFSTDRTNNLIVRLRHNVIRTGLRNISISYSRISLADVAKKLRLDCANPVAD 421

Query: 462 AESIVSKAIRDGAIDATVDHANGWMVSKETGDIYSTNEPQIAFNSRIAFCLNMHNEAVRA 521
           AESIVSKAIRDGAIDATVDHANGWMVSKETGDIY TNEPQIAFNSRIAFCLNMHNEAVRA
Sbjct: 422 AESIVSKAIRDGAIDATVDHANGWMVSKETGDIYLTNEPQIAFNSRIAFCLNMHNEAVRA 481

Query: 522 LRFPPNSHKEKESAEKRRERQQQEQELAKHIAEEDEDDF 561
           LRFPPNSHKEKESAEKRRERQQQEQELAKHIAEED+DDF
Sbjct: 482 LRFPPNSHKEKESAEKRRERQQQEQELAKHIAEEDDDDF 485

BLAST of Spg014773 vs. TAIR 10
Match: AT1G20200.1 (PAM domain (PCI/PINT associated module) protein )

HSP 1 Score: 741.9 bits (1914), Expect = 3.8e-214
Identity = 401/523 (76.67%), Postives = 442/523 (84.51%), Query Frame = 0

Query: 42  TQDAEMKELPAPVSSSSAPTSPSTLQRKKFYLHFVMLVEFLEDLVLKFVNDFFQSDLKEI 101
           TQD EMK+   P S S   +S ST+Q                             +LKEI
Sbjct: 2   TQDVEMKDNNTP-SQSIISSSTSTMQ-----------------------------NLKEI 61

Query: 102 VSLIETGAYAREVRRIVRAIRLTMALRRKLKASVLSSFLNFSLPPGSDVHTRLSSFIPKE 161
            +LI+TG+Y +EVRRI RA+RLT+ LR+KL  SVLSSFL+F+L PGS+ H+RLSSF+PK 
Sbjct: 62  AALIDTGSYTKEVRRIARAVRLTIGLRQKLTGSVLSSFLDFALVPGSEAHSRLSSFVPKG 121

Query: 162 DVYEMDVD---SATQALVKHPLP-EIEIFCYLIVLIFLIDQKKYDEAKACASASIARLKN 221
           D ++M+VD   SATQA     LP E+EI+CY IVL+FLIDQKKY+EAKAC+SASIARLKN
Sbjct: 122 DEHDMEVDTASSATQAAPSKHLPAELEIYCYFIVLLFLIDQKKYNEAKACSSASIARLKN 181

Query: 222 LNRRTVDVLASRLYFYYSLSYELTGDLAEIRGNLLALHRIGTLRHDELGQETLLNLLLRN 281
           +NRRT+DV+ASRLYFYYSLSYE TGDLAEIRG LLALH   TLRHDELGQETLLNLLLRN
Sbjct: 182 VNRRTIDVIASRLYFYYSLSYEQTGDLAEIRGTLLALHHSATLRHDELGQETLLNLLLRN 241

Query: 282 YLHYNLYDQAEKLRSKAPRFEAHSNQQASLSALFCRYLFYLGKIRTIQLEYTDAKESLLQ 341
           YLHYNLYDQAEKLRSKAPRFEAHSNQQ      FCRYLFYLGKIRTIQLEYTDAKESLLQ
Sbjct: 242 YLHYNLYDQAEKLRSKAPRFEAHSNQQ------FCRYLFYLGKIRTIQLEYTDAKESLLQ 301

Query: 342 AARKAPIAALGFRVQCNKWAIIVRLLLGEIPERTVFMQKGMETALRPYFELTNAVRIGDL 401
           AARKAPIAALGFR+QCNKWAI+VRLLLGEIPER++F QKGME ALRPYFELTNAVRIGDL
Sbjct: 302 AARKAPIAALGFRIQCNKWAILVRLLLGEIPERSIFTQKGMEKALRPYFELTNAVRIGDL 361

Query: 402 ELFRTVAEKFSSTFSSDRTNNLIVRLRHNVIRTGLRNISISYSRISLADVAKKLRLDSEN 461
           ELFRTV EKF  TF+ DRT+NLIVRLRHNVIRTGLRNISISYSRISL DVAKKLRL+SEN
Sbjct: 362 ELFRTVQEKFLDTFAQDRTHNLIVRLRHNVIRTGLRNISISYSRISLPDVAKKLRLNSEN 421

Query: 462 PVADAESIVSKAIRDGAIDATVDHANGWMVSKETGDIYSTNEPQIAFNSRIAFCLNMHNE 521
           PVADAESIV+KAIRDGAIDAT+DH NG MVSKETGDIYSTNEPQ AFNSRIAFCLNMHNE
Sbjct: 422 PVADAESIVAKAIRDGAIDATIDHKNGCMVSKETGDIYSTNEPQTAFNSRIAFCLNMHNE 481

Query: 522 AVRALRFPPNSHKEKESAEKRRERQQQEQELAKHIAEEDEDDF 561
           AVRALRFPPN+HKEKES EKRRER+QQE+ELAKH+AEED+DDF
Sbjct: 482 AVRALRFPPNTHKEKESDEKRRERKQQEEELAKHMAEEDDDDF 488

BLAST of Spg014773 vs. TAIR 10
Match: AT1G75990.1 (PAM domain (PCI/PINT associated module) protein )

HSP 1 Score: 723.8 bits (1867), Expect = 1.1e-208
Identity = 389/523 (74.38%), Postives = 437/523 (83.56%), Query Frame = 0

Query: 42  TQDAEMKELPAPVSS-SSAPTSPSTLQRKKFYLHFVMLVEFLEDLVLKFVNDFFQSDLKE 101
           TQD EMK+   P  S  SAPT  STLQ                             +LKE
Sbjct: 2   TQDVEMKDNQTPTQSVVSAPT--STLQ-----------------------------NLKE 61

Query: 102 IVSLIETGAYAREVRRIVRAIRLTMALRRKLKASVLSSFLNFSLPPGSDVHTRLSSFIPK 161
           I +LI+TG+Y +EVRRI RA+RLT+ LRRKL  SV+SSFL+F+L PGS+ HTRLSSF+PK
Sbjct: 62  IAALIDTGSYTKEVRRIARAVRLTVGLRRKLTGSVISSFLDFALVPGSEAHTRLSSFVPK 121

Query: 162 EDVYEMDVDSA---TQALVKHPLPEIEIFCYLIVLIFLIDQKKYDEAKACASASIARLKN 221
            D ++M+VD+A   +QA  KH   E+EI+CY IVL+FLIDQKKY+EAK C++ASIARLK+
Sbjct: 122 SDEHDMEVDTASSNSQAPPKHLPAELEIYCYFIVLLFLIDQKKYNEAKVCSTASIARLKS 181

Query: 222 LNRRTVDVLASRLYFYYSLSYELTGDLAEIRGNLLALHRIGTLRHDELGQETLLNLLLRN 281
           +NRRTVDV+AS+LYFYYSLSYELT DLAEIR  LLALH   TLRHDELGQETLLNLLLRN
Sbjct: 182 VNRRTVDVIASKLYFYYSLSYELTNDLAEIRSTLLALHHSATLRHDELGQETLLNLLLRN 241

Query: 282 YLHYNLYDQAEKLRSKAPRFEAHSNQQASLSALFCRYLFYLGKIRTIQLEYTDAKESLLQ 341
           YLHYNLYDQAEKLRSKAPRFEAHSNQQ      FCRYLFYLGKIRTIQLEYTDAKESLLQ
Sbjct: 242 YLHYNLYDQAEKLRSKAPRFEAHSNQQ------FCRYLFYLGKIRTIQLEYTDAKESLLQ 301

Query: 342 AARKAPIAALGFRVQCNKWAIIVRLLLGEIPERTVFMQKGMETALRPYFELTNAVRIGDL 401
           AARKAP+A+LGFR+QCNKWAIIVRLLLGEIPER++F QKGME  LRPYFELTNAVRIGDL
Sbjct: 302 AARKAPVASLGFRIQCNKWAIIVRLLLGEIPERSIFTQKGMEKTLRPYFELTNAVRIGDL 361

Query: 402 ELFRTVAEKFSSTFSSDRTNNLIVRLRHNVIRTGLRNISISYSRISLADVAKKLRLDSEN 461
           ELF  + EKF+ TF+ DRT+NLIVRLRHNVIRTGLRNISISYSRISL DVA+KLRL+S N
Sbjct: 362 ELFGKIQEKFAKTFAEDRTHNLIVRLRHNVIRTGLRNISISYSRISLQDVAQKLRLNSAN 421

Query: 462 PVADAESIVSKAIRDGAIDATVDHANGWMVSKETGDIYSTNEPQIAFNSRIAFCLNMHNE 521
           PVADAESIV+KAIRDGAIDAT+DH NG MVSKETGDIYSTNEPQ AFNSRIAFCLNMHNE
Sbjct: 422 PVADAESIVAKAIRDGAIDATIDHKNGCMVSKETGDIYSTNEPQTAFNSRIAFCLNMHNE 481

Query: 522 AVRALRFPPNSHKEKESAEKRRERQQQEQELAKHIAEEDEDDF 561
           AVRALRFPPN+H+EKES EKRRE +QQE+ELAK++AEED+DDF
Sbjct: 482 AVRALRFPPNTHREKESEEKRREMKQQEEELAKYMAEEDDDDF 487