Spg014727 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg014727
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionPlant protein of unknown function (DUF946)
Locationscaffold3: 41160828 .. 41165329 (-)
RNA-Seq ExpressionSpg014727
SyntenySpg014727
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GCGACCACACGCGTCTCAGAAAATCATTGGTTGAATGTTCAATTGGTGGAGATGAAGTGTTTGATTTATTTTATTTTTGTTCTATCGATGATTGATTGATCGGGAGAGTCCACACAAATCGGTTAAACCTTTCTTTTCCTTTCAATCTTTTTCTTTGTTCGGTATAGTTTCCCTCCATATCAAGATAAATAGTTATTCTTCTCCTTCATTACAGAGATTTCCCGAATGAGATCAATCCTTAACTTTCGCTCTGCAAATTTTCCTTATTCCCGAATGATTTAACGTACCGCCATAGCTTAAAAATGGTGACGAATTTGTTGGTTTCCATCTTCCACCGCTCGCCAATGCCACGAACTTCCAGGTGCGGCGTCCTTGTGCTTTTGGATGTTTTTCGTTTACAGTTCCATTTCTGGGTTTTTATTTACGAACAGCTTCTTACTGTACTTACCCACGAACTCCACAAACGAACATGGAATTGATCGCCCCACATTTTCAGGAATCTGGGTTCTTTGATTTTTTGTTCTTTTTGAATGTTTTGATTGTTTAGAAGGTTTAATTTCCACTTTCCCAGCTCCGTTTTCTCCATTACCAAAATTTAACTGTTGAAAATCATTGTCGTTGAAATCGATTATCGGTTTTCCAAAAGGATGCTGTCGAACATGATGATCTCTGATCAAGGGTTGATTGCAGCAGTTCCATAAAGCCATCAGAAGGGGTGAATGAATCCTGGGTTCGTAAAGCAGCGTTGTTTGGTGTTTATTCCTGCTACTTGTGGCTTAGCTTTGAGTGCTTTAGATGACCTCGCCATTTATCATAGCTGTAGCAGGTATGTCTGAATTGTTAGACTTAGAGCTTTCCAAATTCTGGATGAACTATAGAGTTTACTGAAATGGAGGTCTAAATTGTTATACTTACTGCTACTTGTTTTTTATGCATTTGTTTGCTGCCATGTTTCACTTATTTTTGCTTCGAAGTTCAAGGAAACTATTCCCATCACCATGCTGTCGTCTTCATAGCAGTCTAACTTAAAAAGGCAAGAGAGTATCGAAGCTGTTGAGTCTCGTTAGTTCTTTACCTTTAAAATTGGCTATGGTTTTACATTACTCAGGTCATTGTACTTTCTAAATCGAGTCTTTTTTTTTTTTTTTTTGAAAAAGTTCTAAATCGAGTCTTTTCTTCACTTTAGATGAACTAATTAATTTGATCAGATGATGTCTCGTATTAGTATGCAATGACATTTACAATATCATTACTATAAGAGTTCAGTTGTCAATCTTTTTACGATGAGGGGCAAGAGAAAGCAAGATTTTCTTTTCTTTCTTTCTTCTTCTTCTTTTTTTTTTTTTTTTTTTTTTTGGGTATAACTTTTCCTTATTCCTGAATGGTTTAACCCAGATGACGTCCGGATGCAACTGGTTCCATTGGAGCAATGCCCACTACCTTTTGCCATCGGATGAGCCTGACCATTTCTCGTTGCCTTCCCCAATTCCTGAATGGCCTCAAGGTATATGATACTCTCAAATGAAAATGAGTTATTGGCTTTTGTTCATTTTTTTGTTTTATATTTTCCCTTGTTACTTCTCATTGCACTTGAGGCAATTTTTTGTTTGTTTCTGACTCTTTGTGTGGCTTTAGTTGATAAGGAGTGTTGGACAAACTAGTTGATCTCTCCTTTTCCCCCAATAAACTAATTGTGTTTGTAGTGTAAGATTTCTAACGTTGAAGCAAGATAGAAATATAATGTTGATCTCTTGTTGAAAACTATGAGGCAGTTGTGTTATATTGCCTTCCATTTCGAGCTTGAAGGAAGCTCGAAATAGTCCTTTGGAAGGAAGTACTGTCAAAATTTAACCATCACATCGAGAAAAGATATCTGCAATGAGAACTAGATTGTTTTAATGCAAAATGTGATCCGATCACACAATGTAAAAGTATGCCAGTAGAGCAGAATATCCATTTATATTTGAGGTCACTATAGCTTGGCAAGTTGTAGGATTGATTTCAGTGGGGGAAAAATCTTGAAGAAATTGAAAATCTGTGTGGTGATCTTTGACGTAGTAATACTCGAGTTCTGTTTTTGTTTTTTTACAAGTAGTATTTGGACTTCCCCACTTCCAAAACACACACACTCACCAGCCGTAATAATATTCATTGATATAATTGAATCATTTAGAGAAAAAGATATCCTCTTCATATCATCTTCGATGATGTTCCTACTGAGTTTATTGTCAGCCTACATTCTCTCAATATTAATAGCCTGTCTCATTTTCATTTCAAGGAAAAAAAAAAAGTTAACAGCTGCCTCTAGTTTCTAAACATGTTGAGTTTGCCAAGTTCTGTAACTTGTGAATAAGTAATTAGGAAGTCGAGACTCGAGAGTTTTTACTTGTGTAGTTGCAGTTATATATACAAATATTGATCCTCCTCTAATTGAACTTGATGCATATCTTTCTAGGTGGAGGTTTTGCTTCTGGAATAGCAAGTCTTGGGGAAATTGAGGTTCTGAAAATCACCCAATTTGTTTCCATATGGGGCTACAATCTCACACGCCGAGAAAATAATGGCGTCACATTTTACAGACCATTAAGGATACCTGAAGGATTCCACTGCCTTGGTCACTATTGCCAACCTAATGACAAGCCCCTGCATGGTTATCTTCTTGTGGCAAAGGAAGTAGATGCTTCTGTCCAGGAAAGTGATCATATTAGCAACATAGTTAAATTACCAGCCCTTGTGGAACCCCTTGATTATGCATTGATATGGAGTCCAGATGATGGGAGTGAAGAGAAGTACAGTGAATGTGCCTACATTTGGCTTCCTCAACCACCTGATGGTTATAAATCCATGGGTTATTTTGTAACTAACAAGCTAAAGAAGCCTGAATTGAGTGAAGTAAGGTGTGTTCGAGCTGATTTAACCGATAGGTGCGAAACTTACCGCTTAATGCTTAATATCAGTTTTAAATGTACAAACTTTCTAGTACAGATTTGGAGTACAAGAGCTTGTCACCGGGGAATGCTTGGTAGGGGAGTTCCTGTAGGAACATTTTATTGTGGTAGTTATAAAAGCGCTGAAAAAGAGCTTCCTATTGCATGCTTAAAAAACCTGGATTCTACGCTATCTACAATGCCGAACCTCGATCAGATTCACGCTCTTATCCGCCACTATGGACCCACTCTCTTTTTTCATCCTAAAGAGATCTACTTGCCATCTTCTGTTTCATGGTTTTTCGAAAATGGTGTGCGATTACACAGAGATGGTTTGTCATCTGGGGAGGCCATACATGTTTGTGGCACAAATTTGCCAGTTGGTGGGAGAAACGATAAGGAATGTTGGATGGATTTGCCGACTGATGGCTGTAGAGACAAGATCATATATGGAAATCTGGAAAGCGCGAAACTTTACGTTCATGTGAAGCCAGCTCTGGGTGGGACATTCACGGATATTGCTTTGTGGATTTTCTGTCCCTTCAATGGACCAGCCACTCTTAAGCTTGGAATCATGAATATTAGTCTTGGGAAAATTGGACAACATGTGGGGGATTGGGAGCATTTCACCCTCAGGATCTGCAACTTTACAGGACAACTTTATAGCATTTACTTCTCCCAGCACAGTGGTGGTGAGTGGGTGGATCCTTACAATTTGGAATTCATTGAAGGGAACAAAGCAACAGTTTACTCCTCAAAGAGTGGACACGCTAGCTATGCTCATCCTGGGGTCTACATTCAAGGCTCGGCGAAGCTCGGGATCGGAATAAGGAATGACTGCGCACGTAGTCATTTTTTGATTGATTCAAGCATCCATTATGAAATAGTTGCAGCAGAGTACCTAAAAAGCAATGACATTGTGGAGCCTTGTTGGTTGCAGTTCATGAGAGAATGGGGTCCAACTATTGTCTACAGCTCGAGAACGAGGCTCGACAAAATGATCGATCGCCTTCCGTCGACAATTCGGTTTTCTGTTGCAAATATATTCAGAAAGTTGCCTGCGGAATTGTTTGGAGAGGGTGGTCCAACTGGACCAAAGGAGAAGGACAATTGGGAAGGAGATGAGAGAGGCTAAAACGCTCTTGTGCATTCTACCTTTGTTGCTGCTAGTGTGGTAAAAAAGCTATGTTAGTGTTTGTACGGCCGTTTGAGACATGTTTGAAAGTGATTTTAGAATGACCAAAATCACTTTTGTTATGCCCCTTAATGATTTTAGACATGACAAAAGTGATTTTGACATTCCAAAATCACTCCCGAACATGTCTTTTTTCTTCTAGTTAGGGACAAGAAAAATTGATTGGGGTTGGATTTAGTATCTAGAAAACCCAACTCAATTCTAATAGAATTCATATTTTTTTTCTTTGAAGTATTGATTCTTGGTTGAGCAAACATTAGGCAGTAGAGATGAGTCTCAGCCTTTCACAATTATAATGTAATTATAATGTAACCTGTAGTTGACAAACATTTTATACCAAATAGGGAAAGAAGCATTACAAAAAATAAATAGTTGAGGAA

mRNA sequence

ATGAATCCTGGGTTCGTAAAGCAGCGTTGTTTGGTGTTTATTCCTGCTACTTGTGGCTTAGCTTTGAGTGCTTTAGATGACCTCGCCATTTATCATAGCTGTAGCAGCAATGCCCACTACCTTTTGCCATCGGATGAGCCTGACCATTTCTCGTTGCCTTCCCCAATTCCTGAATGGCCTCAAGGTGGAGGTTTTGCTTCTGGAATAGCAAGTCTTGGGGAAATTGAGGTTCTGAAAATCACCCAATTTGTTTCCATATGGGGCTACAATCTCACACGCCGAGAAAATAATGGCGTCACATTTTACAGACCATTAAGGATACCTGAAGGATTCCACTGCCTTGGTCACTATTGCCAACCTAATGACAAGCCCCTGCATGGTTATCTTCTTGTGGCAAAGGAAGTAGATGCTTCTGTCCAGGAAAGTGATCATATTAGCAACATAGTTAAATTACCAGCCCTTGTGGAACCCCTTGATTATGCATTGATATGGAGTCCAGATGATGGGAGTGAAGAGAAGTACAGTGAATGTGCCTACATTTGGCTTCCTCAACCACCTGATGGTTATAAATCCATGGGTTATTTTGTAACTAACAAGCTAAAGAAGCCTGAATTGAGTGAAGTAAGGTGTGTTCGAGCTGATTTAACCGATAGGTGCGAAACTTACCGCTTAATGCTTAATATCAGTTTTAAATGTACAAACTTTCTAGTACAGATTTGGAGTACAAGAGCTTGTCACCGGGGAATGCTTGGTAGGGGAGTTCCTGTAGGAACATTTTATTGTGGTAGTTATAAAAGCGCTGAAAAAGAGCTTCCTATTGCATGCTTAAAAAACCTGGATTCTACGCTATCTACAATGCCGAACCTCGATCAGATTCACGCTCTTATCCGCCACTATGGACCCACTCTCTTTTTTCATCCTAAAGAGATCTACTTGCCATCTTCTGTTTCATGGTTTTTCGAAAATGGTGTGCGATTACACAGAGATGGTTTGTCATCTGGGGAGGCCATACATGTTTGTGGCACAAATTTGCCAGTTGGTGGGAGAAACGATAAGGAATGTTGGATGGATTTGCCGACTGATGGCTGTAGAGACAAGATCATATATGGAAATCTGGAAAGCGCGAAACTTTACGTTCATGTGAAGCCAGCTCTGGGTGGGACATTCACGGATATTGCTTTGTGGATTTTCTGTCCCTTCAATGGACCAGCCACTCTTAAGCTTGGAATCATGAATATTAGTCTTGGGAAAATTGGACAACATGTGGGGGATTGGGAGCATTTCACCCTCAGGATCTGCAACTTTACAGGACAACTTTATAGCATTTACTTCTCCCAGCACAGTGGTGGTGAGTGGGTGGATCCTTACAATTTGGAATTCATTGAAGGGAACAAAGCAACAGTTTACTCCTCAAAGAGTGGACACGCTAGCTATGCTCATCCTGGGGTCTACATTCAAGGCTCGGCGAAGCTCGGGATCGGAATAAGGAATGACTGCGCACGTAGTCATTTTTTGATTGATTCAAGCATCCATTATGAAATAGTTGCAGCAGAGTACCTAAAAAGCAATGACATTGTGGAGCCTTGTTGGTTGCAGTTCATGAGAGAATGGGGTCCAACTATTGTCTACAGCTCGAGAACGAGGCTCGACAAAATGATCGATCGCCTTCCGTCGACAATTCGGTTTTCTGTTGCAAATATATTCAGAAAGTTGCCTGCGGAATTGTTTGGAGAGGGTGGTCCAACTGGACCAAAGGAGAAGGACAATTGGGAAGGAGATGAGAGAGGCTAA

Coding sequence (CDS)

ATGAATCCTGGGTTCGTAAAGCAGCGTTGTTTGGTGTTTATTCCTGCTACTTGTGGCTTAGCTTTGAGTGCTTTAGATGACCTCGCCATTTATCATAGCTGTAGCAGCAATGCCCACTACCTTTTGCCATCGGATGAGCCTGACCATTTCTCGTTGCCTTCCCCAATTCCTGAATGGCCTCAAGGTGGAGGTTTTGCTTCTGGAATAGCAAGTCTTGGGGAAATTGAGGTTCTGAAAATCACCCAATTTGTTTCCATATGGGGCTACAATCTCACACGCCGAGAAAATAATGGCGTCACATTTTACAGACCATTAAGGATACCTGAAGGATTCCACTGCCTTGGTCACTATTGCCAACCTAATGACAAGCCCCTGCATGGTTATCTTCTTGTGGCAAAGGAAGTAGATGCTTCTGTCCAGGAAAGTGATCATATTAGCAACATAGTTAAATTACCAGCCCTTGTGGAACCCCTTGATTATGCATTGATATGGAGTCCAGATGATGGGAGTGAAGAGAAGTACAGTGAATGTGCCTACATTTGGCTTCCTCAACCACCTGATGGTTATAAATCCATGGGTTATTTTGTAACTAACAAGCTAAAGAAGCCTGAATTGAGTGAAGTAAGGTGTGTTCGAGCTGATTTAACCGATAGGTGCGAAACTTACCGCTTAATGCTTAATATCAGTTTTAAATGTACAAACTTTCTAGTACAGATTTGGAGTACAAGAGCTTGTCACCGGGGAATGCTTGGTAGGGGAGTTCCTGTAGGAACATTTTATTGTGGTAGTTATAAAAGCGCTGAAAAAGAGCTTCCTATTGCATGCTTAAAAAACCTGGATTCTACGCTATCTACAATGCCGAACCTCGATCAGATTCACGCTCTTATCCGCCACTATGGACCCACTCTCTTTTTTCATCCTAAAGAGATCTACTTGCCATCTTCTGTTTCATGGTTTTTCGAAAATGGTGTGCGATTACACAGAGATGGTTTGTCATCTGGGGAGGCCATACATGTTTGTGGCACAAATTTGCCAGTTGGTGGGAGAAACGATAAGGAATGTTGGATGGATTTGCCGACTGATGGCTGTAGAGACAAGATCATATATGGAAATCTGGAAAGCGCGAAACTTTACGTTCATGTGAAGCCAGCTCTGGGTGGGACATTCACGGATATTGCTTTGTGGATTTTCTGTCCCTTCAATGGACCAGCCACTCTTAAGCTTGGAATCATGAATATTAGTCTTGGGAAAATTGGACAACATGTGGGGGATTGGGAGCATTTCACCCTCAGGATCTGCAACTTTACAGGACAACTTTATAGCATTTACTTCTCCCAGCACAGTGGTGGTGAGTGGGTGGATCCTTACAATTTGGAATTCATTGAAGGGAACAAAGCAACAGTTTACTCCTCAAAGAGTGGACACGCTAGCTATGCTCATCCTGGGGTCTACATTCAAGGCTCGGCGAAGCTCGGGATCGGAATAAGGAATGACTGCGCACGTAGTCATTTTTTGATTGATTCAAGCATCCATTATGAAATAGTTGCAGCAGAGTACCTAAAAAGCAATGACATTGTGGAGCCTTGTTGGTTGCAGTTCATGAGAGAATGGGGTCCAACTATTGTCTACAGCTCGAGAACGAGGCTCGACAAAATGATCGATCGCCTTCCGTCGACAATTCGGTTTTCTGTTGCAAATATATTCAGAAAGTTGCCTGCGGAATTGTTTGGAGAGGGTGGTCCAACTGGACCAAAGGAGAAGGACAATTGGGAAGGAGATGAGAGAGGCTAA

Protein sequence

MNPGFVKQRCLVFIPATCGLALSALDDLAIYHSCSSNAHYLLPSDEPDHFSLPSPIPEWPQGGGFASGIASLGEIEVLKITQFVSIWGYNLTRRENNGVTFYRPLRIPEGFHCLGHYCQPNDKPLHGYLLVAKEVDASVQESDHISNIVKLPALVEPLDYALIWSPDDGSEEKYSECAYIWLPQPPDGYKSMGYFVTNKLKKPELSEVRCVRADLTDRCETYRLMLNISFKCTNFLVQIWSTRACHRGMLGRGVPVGTFYCGSYKSAEKELPIACLKNLDSTLSTMPNLDQIHALIRHYGPTLFFHPKEIYLPSSVSWFFENGVRLHRDGLSSGEAIHVCGTNLPVGGRNDKECWMDLPTDGCRDKIIYGNLESAKLYVHVKPALGGTFTDIALWIFCPFNGPATLKLGIMNISLGKIGQHVGDWEHFTLRICNFTGQLYSIYFSQHSGGEWVDPYNLEFIEGNKATVYSSKSGHASYAHPGVYIQGSAKLGIGIRNDCARSHFLIDSSIHYEIVAAEYLKSNDIVEPCWLQFMREWGPTIVYSSRTRLDKMIDRLPSTIRFSVANIFRKLPAELFGEGGPTGPKEKDNWEGDERG
Homology
BLAST of Spg014727 vs. NCBI nr
Match: XP_038876230.1 (uncharacterized protein LOC120068509 isoform X1 [Benincasa hispida] >XP_038876231.1 uncharacterized protein LOC120068509 isoform X1 [Benincasa hispida] >XP_038876232.1 uncharacterized protein LOC120068509 isoform X1 [Benincasa hispida])

HSP 1 Score: 1090.5 bits (2819), Expect = 0.0e+00
Identity = 507/587 (86.37%), Postives = 533/587 (90.80%), Query Frame = 0

Query: 14  IPATCGLALSALDDLAIYHSCS----SNAHYLLPSDEPDHFSLPSPIPEWPQGGGFASGI 73
           +P  CG AL     L +   C+    SN HYLLPSDEPD+FSLPSP PEWPQGGGFASGI
Sbjct: 1   MPPQCGFAL-----LDMASGCNWFHWSNVHYLLPSDEPDYFSLPSPTPEWPQGGGFASGI 60

Query: 74  ASLGEIEVLKITQFVSIWGYNLTRRENNGVTFYRPLRIPEGFHCLGHYCQPNDKPLHGYL 133
           ASLGEIEVLKITQFVSIWG NLTRR NNGVTFYRPLRIPEGFHCLGHYCQPND+PLHGYL
Sbjct: 61  ASLGEIEVLKITQFVSIWGCNLTRRGNNGVTFYRPLRIPEGFHCLGHYCQPNDRPLHGYL 120

Query: 134 LVAKEVDASVQESDHISNIVKLPALVEPLDYALIWSPDDGSEEKYSECAYIWLPQPPDGY 193
           LVA+EVD   QESD+ISNIVKLPALVEPLDY LIWSPDD  EEKYSECAYIWLPQPPDGY
Sbjct: 121 LVAREVDGYFQESDYISNIVKLPALVEPLDYTLIWSPDDWFEEKYSECAYIWLPQPPDGY 180

Query: 194 KSMGYFVTNKLKKPELSEVRCVRADLTDRCETYRLMLNISFKCTNFLVQIWSTRACHRGM 253
           KSMGYFVTNKL+KPE+ EVRCVRADLTD+CETYRLM NIS KC NFLVQIWSTRACHRGM
Sbjct: 181 KSMGYFVTNKLEKPEVGEVRCVRADLTDKCETYRLMFNISTKCKNFLVQIWSTRACHRGM 240

Query: 254 LGRGVPVGTFYCGSYKSAEKELPIACLKNLDSTLSTMPNLDQIHALIRHYGPTLFFHPKE 313
           LGRGVPVGTF+C SYK  EKELPIACLKNLDSTL TMPNL+QIHALI HYGPT+FFHPKE
Sbjct: 241 LGRGVPVGTFHCCSYKGTEKELPIACLKNLDSTLHTMPNLNQIHALINHYGPTIFFHPKE 300

Query: 314 IYLPSSVSWFFENGVRLHRDGLSSGEAIHVCGTNLPVGGRNDKECWMDLPTDGCRDKIIY 373
           IY PSSVSWFFENGV LHRDG+SSGEAIHVCGTNLP GGRND+ECWMDLPTDGCRDKIIY
Sbjct: 301 IYFPSSVSWFFENGVLLHRDGMSSGEAIHVCGTNLPGGGRNDRECWMDLPTDGCRDKIIY 360

Query: 374 GNLESAKLYVHVKPALGGTFTDIALWIFCPFNGPATLKLGIMNISLGKIGQHVGDWEHFT 433
           GNLESAKLYVHVKPALGGTFTD+A+W+FCPFNGPATLKLGIMNISLGKIGQHVGDWEH T
Sbjct: 361 GNLESAKLYVHVKPALGGTFTDMAMWVFCPFNGPATLKLGIMNISLGKIGQHVGDWEHIT 420

Query: 434 LRICNFTGQLYSIYFSQHSGGEWVDPYNLEFIEGNKATVYSSKSGHASYAHPGVYIQGSA 493
           LRICNF+G+L SIYFSQHSGGEWVD +NLEFIEGNKA VYSSKSGHASY HPGVYIQGSA
Sbjct: 421 LRICNFSGELCSIYFSQHSGGEWVDAHNLEFIEGNKAIVYSSKSGHASYPHPGVYIQGSA 480

Query: 494 KLGIGIRNDCARSHFLIDSSIHYEIVAAEYLKSNDIVEPCWLQFMREWGPTIVYSSRTRL 553
           KLGIGIRNDCARSHF +DSSIHYEIVAAEYL+ N I+EPCWLQF REWGPTIVYS RT+L
Sbjct: 481 KLGIGIRNDCARSHFFVDSSIHYEIVAAEYLRCNGIMEPCWLQFTREWGPTIVYSLRTKL 540

Query: 554 DKMIDRLPSTIRFSVANIFRKLPAELFGEGGPTGPKEKDNWEGDERG 597
           D MID LP  IR SVANIFRKLP ELFGEGGPTGPKEK+NWEGDERG
Sbjct: 541 DNMIDHLPLRIRLSVANIFRKLPGELFGEGGPTGPKEKNNWEGDERG 582

BLAST of Spg014727 vs. NCBI nr
Match: XP_038876233.1 (uncharacterized protein LOC120068509 isoform X2 [Benincasa hispida])

HSP 1 Score: 1088.2 bits (2813), Expect = 0.0e+00
Identity = 500/561 (89.13%), Postives = 523/561 (93.23%), Query Frame = 0

Query: 36  SNAHYLLPSDEPDHFSLPSPIPEWPQGGGFASGIASLGEIEVLKITQFVSIWGYNLTRRE 95
           SN HYLLPSDEPD+FSLPSP PEWPQGGGFASGIASLGEIEVLKITQFVSIWG NLTRR 
Sbjct: 11  SNVHYLLPSDEPDYFSLPSPTPEWPQGGGFASGIASLGEIEVLKITQFVSIWGCNLTRRG 70

Query: 96  NNGVTFYRPLRIPEGFHCLGHYCQPNDKPLHGYLLVAKEVDASVQESDHISNIVKLPALV 155
           NNGVTFYRPLRIPEGFHCLGHYCQPND+PLHGYLLVA+EVD   QESD+ISNIVKLPALV
Sbjct: 71  NNGVTFYRPLRIPEGFHCLGHYCQPNDRPLHGYLLVAREVDGYFQESDYISNIVKLPALV 130

Query: 156 EPLDYALIWSPDDGSEEKYSECAYIWLPQPPDGYKSMGYFVTNKLKKPELSEVRCVRADL 215
           EPLDY LIWSPDD  EEKYSECAYIWLPQPPDGYKSMGYFVTNKL+KPE+ EVRCVRADL
Sbjct: 131 EPLDYTLIWSPDDWFEEKYSECAYIWLPQPPDGYKSMGYFVTNKLEKPEVGEVRCVRADL 190

Query: 216 TDRCETYRLMLNISFKCTNFLVQIWSTRACHRGMLGRGVPVGTFYCGSYKSAEKELPIAC 275
           TD+CETYRLM NIS KC NFLVQIWSTRACHRGMLGRGVPVGTF+C SYK  EKELPIAC
Sbjct: 191 TDKCETYRLMFNISTKCKNFLVQIWSTRACHRGMLGRGVPVGTFHCCSYKGTEKELPIAC 250

Query: 276 LKNLDSTLSTMPNLDQIHALIRHYGPTLFFHPKEIYLPSSVSWFFENGVRLHRDGLSSGE 335
           LKNLDSTL TMPNL+QIHALI HYGPT+FFHPKEIY PSSVSWFFENGV LHRDG+SSGE
Sbjct: 251 LKNLDSTLHTMPNLNQIHALINHYGPTIFFHPKEIYFPSSVSWFFENGVLLHRDGMSSGE 310

Query: 336 AIHVCGTNLPVGGRNDKECWMDLPTDGCRDKIIYGNLESAKLYVHVKPALGGTFTDIALW 395
           AIHVCGTNLP GGRND+ECWMDLPTDGCRDKIIYGNLESAKLYVHVKPALGGTFTD+A+W
Sbjct: 311 AIHVCGTNLPGGGRNDRECWMDLPTDGCRDKIIYGNLESAKLYVHVKPALGGTFTDMAMW 370

Query: 396 IFCPFNGPATLKLGIMNISLGKIGQHVGDWEHFTLRICNFTGQLYSIYFSQHSGGEWVDP 455
           +FCPFNGPATLKLGIMNISLGKIGQHVGDWEH TLRICNF+G+L SIYFSQHSGGEWVD 
Sbjct: 371 VFCPFNGPATLKLGIMNISLGKIGQHVGDWEHITLRICNFSGELCSIYFSQHSGGEWVDA 430

Query: 456 YNLEFIEGNKATVYSSKSGHASYAHPGVYIQGSAKLGIGIRNDCARSHFLIDSSIHYEIV 515
           +NLEFIEGNKA VYSSKSGHASY HPGVYIQGSAKLGIGIRNDCARSHF +DSSIHYEIV
Sbjct: 431 HNLEFIEGNKAIVYSSKSGHASYPHPGVYIQGSAKLGIGIRNDCARSHFFVDSSIHYEIV 490

Query: 516 AAEYLKSNDIVEPCWLQFMREWGPTIVYSSRTRLDKMIDRLPSTIRFSVANIFRKLPAEL 575
           AAEYL+ N I+EPCWLQF REWGPTIVYS RT+LD MID LP  IR SVANIFRKLP EL
Sbjct: 491 AAEYLRCNGIMEPCWLQFTREWGPTIVYSLRTKLDNMIDHLPLRIRLSVANIFRKLPGEL 550

Query: 576 FGEGGPTGPKEKDNWEGDERG 597
           FGEGGPTGPKEK+NWEGDERG
Sbjct: 551 FGEGGPTGPKEKNNWEGDERG 571

BLAST of Spg014727 vs. NCBI nr
Match: XP_008459966.1 (PREDICTED: uncharacterized protein LOC103498924 [Cucumis melo] >XP_008459967.1 PREDICTED: uncharacterized protein LOC103498924 [Cucumis melo])

HSP 1 Score: 1087.0 bits (2810), Expect = 0.0e+00
Identity = 496/561 (88.41%), Postives = 525/561 (93.58%), Query Frame = 0

Query: 36  SNAHYLLPSDEPDHFSLPSPIPEWPQGGGFASGIASLGEIEVLKITQFVSIWGYNLTRRE 95
           SNAHYLLPSDEPDHFSLPSP PEWPQGGGFASGIASLGEIEVLKITQFVSIWG NL+RR 
Sbjct: 11  SNAHYLLPSDEPDHFSLPSPTPEWPQGGGFASGIASLGEIEVLKITQFVSIWGCNLSRRG 70

Query: 96  NNGVTFYRPLRIPEGFHCLGHYCQPNDKPLHGYLLVAKEVDASVQESDHISNIVKLPALV 155
           NNG TFYRPLRIPEGFHCLGHYCQPND+PLHGYLLVA+EVD   QESDHISNIVKLPALV
Sbjct: 71  NNGFTFYRPLRIPEGFHCLGHYCQPNDRPLHGYLLVAREVDGYFQESDHISNIVKLPALV 130

Query: 156 EPLDYALIWSPDDGSEEKYSECAYIWLPQPPDGYKSMGYFVTNKLKKPELSEVRCVRADL 215
           EP+D+ LIWSPDDGSEEKY EC YIWLPQPPDGYKSMGYFVTNKL+KPE+ EVRCVRADL
Sbjct: 131 EPIDFTLIWSPDDGSEEKYGECVYIWLPQPPDGYKSMGYFVTNKLEKPEVGEVRCVRADL 190

Query: 216 TDRCETYRLMLNISFKCTNFLVQIWSTRACHRGMLGRGVPVGTFYCGSYKSAEKELPIAC 275
           TDRCETYRLM NIS KC NFLVQIWSTRACHRGMLGRGVPVGTF+C SYK  EKELPIAC
Sbjct: 191 TDRCETYRLMFNISSKCKNFLVQIWSTRACHRGMLGRGVPVGTFHCCSYKGTEKELPIAC 250

Query: 276 LKNLDSTLSTMPNLDQIHALIRHYGPTLFFHPKEIYLPSSVSWFFENGVRLHRDGLSSGE 335
           LKNLDSTL TMPN++QIH+LI HYGPT+FFHP+EIYLPSSVSWFFENGV LHRDG+SSGE
Sbjct: 251 LKNLDSTLPTMPNINQIHSLINHYGPTVFFHPEEIYLPSSVSWFFENGVLLHRDGMSSGE 310

Query: 336 AIHVCGTNLPVGGRNDKECWMDLPTDGCRDKIIYGNLESAKLYVHVKPALGGTFTDIALW 395
           AIHVCGTNLP GGRND  CWMDLPTDGCRDKIIYGNLESAKLYVHVKPALGGTFTDIA+W
Sbjct: 311 AIHVCGTNLPAGGRNDTVCWMDLPTDGCRDKIIYGNLESAKLYVHVKPALGGTFTDIAMW 370

Query: 396 IFCPFNGPATLKLGIMNISLGKIGQHVGDWEHFTLRICNFTGQLYSIYFSQHSGGEWVDP 455
           +FCPFNGP+TLKLGI+NISLGKIGQHVGDWEH TLRICNF+G+L+SIYFSQHSGGEWVD 
Sbjct: 371 VFCPFNGPSTLKLGIVNISLGKIGQHVGDWEHITLRICNFSGELFSIYFSQHSGGEWVDA 430

Query: 456 YNLEFIEGNKATVYSSKSGHASYAHPGVYIQGSAKLGIGIRNDCARSHFLIDSSIHYEIV 515
           YNLEFIEGNKA VYSSKSGHASY HPG+YIQGS+KLGIGIRNDCARSH  IDSSIHYEIV
Sbjct: 431 YNLEFIEGNKAIVYSSKSGHASYPHPGLYIQGSSKLGIGIRNDCARSHLFIDSSIHYEIV 490

Query: 516 AAEYLKSNDIVEPCWLQFMREWGPTIVYSSRTRLDKMIDRLPSTIRFSVANIFRKLPAEL 575
           AAE+L+ N+IVEPCWLQFMREWGPTIVYSSRT+LD  IDRLP  IR +VANIFRKLPAEL
Sbjct: 491 AAEHLRCNEIVEPCWLQFMREWGPTIVYSSRTKLDNFIDRLPLKIRLTVANIFRKLPAEL 550

Query: 576 FGEGGPTGPKEKDNWEGDERG 597
           FGE GPTGPKEK+NWEGDERG
Sbjct: 551 FGEVGPTGPKEKNNWEGDERG 571

BLAST of Spg014727 vs. NCBI nr
Match: XP_004140668.1 (uncharacterized protein LOC101209282 isoform X2 [Cucumis sativus] >XP_011656767.1 uncharacterized protein LOC101209282 isoform X2 [Cucumis sativus] >XP_011656768.1 uncharacterized protein LOC101209282 isoform X2 [Cucumis sativus] >KGN46379.2 hypothetical protein Csa_004970 [Cucumis sativus])

HSP 1 Score: 1073.5 bits (2775), Expect = 4.6e-310
Identity = 493/561 (87.88%), Postives = 520/561 (92.69%), Query Frame = 0

Query: 36  SNAHYLLPSDEPDHFSLPSPIPEWPQGGGFASGIASLGEIEVLKITQFVSIWGYNLTRRE 95
           SNAHYLLPSDEPDHFSLPSP PEWPQGGGFASGIASLGEIEVLKITQFVSIWG NL+RR 
Sbjct: 11  SNAHYLLPSDEPDHFSLPSPTPEWPQGGGFASGIASLGEIEVLKITQFVSIWGCNLSRRG 70

Query: 96  NNGVTFYRPLRIPEGFHCLGHYCQPNDKPLHGYLLVAKEVDASVQESDHISNIVKLPALV 155
           NNGVTFYRPLR+PEG+HCLGHYCQPND+PLHGYLLVA+EVD   QESDHISNIVKLPALV
Sbjct: 71  NNGVTFYRPLRMPEGYHCLGHYCQPNDRPLHGYLLVAREVDGYFQESDHISNIVKLPALV 130

Query: 156 EPLDYALIWSPDDGSEEKYSECAYIWLPQPPDGYKSMGYFVTNKLKKPELSEVRCVRADL 215
           EP+D+ LIWSPDDGSEEKY ECAYIWLPQPPDGYKSMGYFVTNKL+KP + EVRCVRADL
Sbjct: 131 EPIDFTLIWSPDDGSEEKYGECAYIWLPQPPDGYKSMGYFVTNKLEKPVVGEVRCVRADL 190

Query: 216 TDRCETYRLMLNISFKCTNFLVQIWSTRACHRGMLGRGVPVGTFYCGSYKSAEKELPIAC 275
           TDRCETYRLM NIS KC NFLVQIWSTRACHRGMLGRGVPVGTF+CGSYK  EKELPIAC
Sbjct: 191 TDRCETYRLMFNISSKCKNFLVQIWSTRACHRGMLGRGVPVGTFHCGSYKGTEKELPIAC 250

Query: 276 LKNLDSTLSTMPNLDQIHALIRHYGPTLFFHPKEIYLPSSVSWFFENGVRLHRDGLSSGE 335
           LKNL+STL TMPN+DQIH+LI HYGPT+FFHPKEIYLPSSVSWFFENGV LHRDG+SSGE
Sbjct: 251 LKNLNSTLPTMPNIDQIHSLINHYGPTVFFHPKEIYLPSSVSWFFENGVLLHRDGMSSGE 310

Query: 336 AIHVCGTNLPVGGRNDKECWMDLPTDGCRDKIIYGNLESAKLYVHVKPALGGTFTDIALW 395
           AI VCGTNLP  GRND  CWMDLPTDGCRDKII GNLESAKLY HVKPALGGTFTDIA+W
Sbjct: 311 AILVCGTNLPTDGRNDTVCWMDLPTDGCRDKIINGNLESAKLYAHVKPALGGTFTDIAMW 370

Query: 396 IFCPFNGPATLKLGIMNISLGKIGQHVGDWEHFTLRICNFTGQLYSIYFSQHSGGEWVDP 455
           +FCPFNGP+TLKLGI+NISLGKIGQHVGDWEH TLRICNFTG+L+SIYFSQHSGGEWVD 
Sbjct: 371 VFCPFNGPSTLKLGIVNISLGKIGQHVGDWEHITLRICNFTGELFSIYFSQHSGGEWVDA 430

Query: 456 YNLEFIEGNKATVYSSKSGHASYAHPGVYIQGSAKLGIGIRNDCARSHFLIDSSIHYEIV 515
           YNLEFIEGNKA VYSSKSGHASY  PG+YIQGS+KLGIGIRNDCARSH  IDSS HYEIV
Sbjct: 431 YNLEFIEGNKAIVYSSKSGHASYPRPGLYIQGSSKLGIGIRNDCARSHLFIDSSTHYEIV 490

Query: 516 AAEYLKSNDIVEPCWLQFMREWGPTIVYSSRTRLDKMIDRLPSTIRFSVANIFRKLPAEL 575
           AAE+L+ NDIVEP WLQFMREWGPTIVYSSRT+LD  IDRLP  IRF VANIFRKLPAEL
Sbjct: 491 AAEHLRRNDIVEPGWLQFMREWGPTIVYSSRTKLDNFIDRLPLKIRFPVANIFRKLPAEL 550

Query: 576 FGEGGPTGPKEKDNWEGDERG 597
           FGE GPTGPKEK+NWEGDERG
Sbjct: 551 FGEVGPTGPKEKNNWEGDERG 571

BLAST of Spg014727 vs. NCBI nr
Match: XP_022960118.1 (uncharacterized protein LOC111460961 [Cucurbita moschata] >XP_022960119.1 uncharacterized protein LOC111460961 [Cucurbita moschata] >XP_022960120.1 uncharacterized protein LOC111460961 [Cucurbita moschata])

HSP 1 Score: 1067.4 bits (2759), Expect = 4.5e-308
Identity = 493/561 (87.88%), Postives = 516/561 (91.98%), Query Frame = 0

Query: 36  SNAHYLLPSDEPDHFSLPSPIPEWPQGGGFASGIASLGEIEVLKITQFVSIWGYNLTRRE 95
           SN H LLPSDEP HFSLPSP+PEWPQGGGF SG ASLGEIEVLKITQF SIWG+NLT RE
Sbjct: 11  SNTHDLLPSDEPYHFSLPSPVPEWPQGGGFDSGKASLGEIEVLKITQFASIWGFNLTHRE 70

Query: 96  NNGVTFYRPLRIPEGFHCLGHYCQPNDKPLHGYLLVAKEVDASVQESDHISNIVKLPALV 155
           NNGVTFYRPLRIPEGFHCLGH+CQ ND+PLHGYLLVA+EVDA  QE DH+SNIVKLPALV
Sbjct: 71  NNGVTFYRPLRIPEGFHCLGHFCQRNDQPLHGYLLVAREVDAYFQEGDHVSNIVKLPALV 130

Query: 156 EPLDYALIWSPDDGSEEKYSECAYIWLPQPPDGYKSMGYFVTNKLKKPELSEVRCVRADL 215
           +PLDY LIWSPDDG EE+YSECAYIWLPQPPDGYKSMGYFVTNKLKKPEL EVRCVRADL
Sbjct: 131 KPLDYTLIWSPDDGREEQYSECAYIWLPQPPDGYKSMGYFVTNKLKKPELGEVRCVRADL 190

Query: 216 TDRCETYRLMLNISFKCTNFLVQIWSTRACHRGMLGRGVPVGTFYCGSYKSAEKELPIAC 275
           TDRCETYRLMLNIS KCTNF VQIWSTRACHRGMLGRGVPVGTFY GS+K  EKELPIAC
Sbjct: 191 TDRCETYRLMLNISLKCTNFPVQIWSTRACHRGMLGRGVPVGTFYSGSHKVTEKELPIAC 250

Query: 276 LKNLDSTLSTMPNLDQIHALIRHYGPTLFFHPKEIYLPSSVSWFFENGVRLHRDGLSSGE 335
           LKNLDSTL TMPNLDQIHALI HYGPT FFHPKEIYLPSSVSWFFENGV LHRDG+SSGE
Sbjct: 251 LKNLDSTLHTMPNLDQIHALINHYGPTFFFHPKEIYLPSSVSWFFENGVLLHRDGMSSGE 310

Query: 336 AIHVCGTNLPVGGRNDKECWMDLPTDGCRDKIIYGNLESAKLYVHVKPALGGTFTDIALW 395
           AIHVCGTNLP GGR++  CWMDLP+D CRDKIIYGNLESAKLYVHVKPALGGTFTDIA+W
Sbjct: 311 AIHVCGTNLPGGGRHETVCWMDLPSDDCRDKIIYGNLESAKLYVHVKPALGGTFTDIAMW 370

Query: 396 IFCPFNGPATLKLGIMNISLGKIGQHVGDWEHFTLRICNFTGQLYSIYFSQHSGGEWVDP 455
           +FCPFNG ATLKLGIM+ISLGKIGQHVGDWEH TLRICNFTG+L SIYFSQHSGGEWVD 
Sbjct: 371 VFCPFNGSATLKLGIMDISLGKIGQHVGDWEHVTLRICNFTGELSSIYFSQHSGGEWVDA 430

Query: 456 YNLEFIEGNKATVYSSKSGHASYAHPGVYIQGSAKLGIGIRNDCARSHFLIDSSIHYEIV 515
           YNLEFI+GNKA VYSSKSGHASY HPGVYIQGSA LGIGIRNDCARSH  IDSS HYEIV
Sbjct: 431 YNLEFIQGNKAIVYSSKSGHASYPHPGVYIQGSAMLGIGIRNDCARSHLCIDSSSHYEIV 490

Query: 516 AAEYLKSNDIVEPCWLQFMREWGPTIVYSSRTRLDKMIDRLPSTIRFSVANIFRKLPAEL 575
           AAEYL+ N +VEPCWLQFMREWGPTI+YSSRT LDKMI+ LPS IRFSVANI  KLP EL
Sbjct: 491 AAEYLRDNGVVEPCWLQFMREWGPTIIYSSRTTLDKMINFLPSMIRFSVANIIGKLPGEL 550

Query: 576 FGEGGPTGPKEKDNWEGDERG 597
           FGEGGPTGPKEK+NWEGDERG
Sbjct: 551 FGEGGPTGPKEKNNWEGDERG 571

BLAST of Spg014727 vs. ExPASy Swiss-Prot
Match: P53285 (Vacuolar protein sorting-associated protein 62 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=VPS62 PE=1 SV=1)

HSP 1 Score: 52.0 bits (123), Expect = 2.7e-05
Identity = 61/255 (23.92%), Postives = 93/255 (36.47%), Query Frame = 0

Query: 386 GGTFTDIALWIFCPFN-GPATLKLGIMNISLGKIGQHVGDWEHFTLRICNFTGQLYSIYF 445
           G  + D   + F PFN GP  +         G  G HVGDWEH  +R   + G+   ++ 
Sbjct: 196 GNGWVDAFWFYFYPFNWGPYIM-------GSGPWGNHVGDWEHSLVRF--YKGEPQYLWM 255

Query: 446 SQHSGGEWVDPYNLEFIEG----------------NKATVYSSKSGHASYAHPGVYIQGS 505
           S H GG     Y  E IE                  K  ++S++  HA YA  G +    
Sbjct: 256 SAHGGG---SAYKFEAIEKIKRLRRVDGKLTNEVIKKPLIFSARGTHAHYASVGQHAHDV 315

Query: 506 AKLGIGIRNDCARSHFLIDSSIHYEIVAAEYLKSNDIVEPC----------WLQFMREWG 565
               + + +   R   L D S++Y    A  +   + + PC          WL F   WG
Sbjct: 316 PFFFMPLSDFTDRGP-LWDPSLNY---YAYTVTVGEKMTPCGAEETKMGLEWLSFKGAWG 375

Query: 566 P-----------------TIVYSSRTRLDKMIDRLPSTIRFSVANIFRKLPAELFGEGGP 597
                               +   +  L K ++R+    RF   N ++  PA  + + G 
Sbjct: 376 DKQLRPRDPRQKWCPFQWKYIDGPKGPLFKNMERVSLCQRFKWWNFWKGCPARRYIKRGE 434

BLAST of Spg014727 vs. ExPASy TrEMBL
Match: A0A1S3CBI0 (uncharacterized protein LOC103498924 OS=Cucumis melo OX=3656 GN=LOC103498924 PE=4 SV=1)

HSP 1 Score: 1087.0 bits (2810), Expect = 0.0e+00
Identity = 496/561 (88.41%), Postives = 525/561 (93.58%), Query Frame = 0

Query: 36  SNAHYLLPSDEPDHFSLPSPIPEWPQGGGFASGIASLGEIEVLKITQFVSIWGYNLTRRE 95
           SNAHYLLPSDEPDHFSLPSP PEWPQGGGFASGIASLGEIEVLKITQFVSIWG NL+RR 
Sbjct: 11  SNAHYLLPSDEPDHFSLPSPTPEWPQGGGFASGIASLGEIEVLKITQFVSIWGCNLSRRG 70

Query: 96  NNGVTFYRPLRIPEGFHCLGHYCQPNDKPLHGYLLVAKEVDASVQESDHISNIVKLPALV 155
           NNG TFYRPLRIPEGFHCLGHYCQPND+PLHGYLLVA+EVD   QESDHISNIVKLPALV
Sbjct: 71  NNGFTFYRPLRIPEGFHCLGHYCQPNDRPLHGYLLVAREVDGYFQESDHISNIVKLPALV 130

Query: 156 EPLDYALIWSPDDGSEEKYSECAYIWLPQPPDGYKSMGYFVTNKLKKPELSEVRCVRADL 215
           EP+D+ LIWSPDDGSEEKY EC YIWLPQPPDGYKSMGYFVTNKL+KPE+ EVRCVRADL
Sbjct: 131 EPIDFTLIWSPDDGSEEKYGECVYIWLPQPPDGYKSMGYFVTNKLEKPEVGEVRCVRADL 190

Query: 216 TDRCETYRLMLNISFKCTNFLVQIWSTRACHRGMLGRGVPVGTFYCGSYKSAEKELPIAC 275
           TDRCETYRLM NIS KC NFLVQIWSTRACHRGMLGRGVPVGTF+C SYK  EKELPIAC
Sbjct: 191 TDRCETYRLMFNISSKCKNFLVQIWSTRACHRGMLGRGVPVGTFHCCSYKGTEKELPIAC 250

Query: 276 LKNLDSTLSTMPNLDQIHALIRHYGPTLFFHPKEIYLPSSVSWFFENGVRLHRDGLSSGE 335
           LKNLDSTL TMPN++QIH+LI HYGPT+FFHP+EIYLPSSVSWFFENGV LHRDG+SSGE
Sbjct: 251 LKNLDSTLPTMPNINQIHSLINHYGPTVFFHPEEIYLPSSVSWFFENGVLLHRDGMSSGE 310

Query: 336 AIHVCGTNLPVGGRNDKECWMDLPTDGCRDKIIYGNLESAKLYVHVKPALGGTFTDIALW 395
           AIHVCGTNLP GGRND  CWMDLPTDGCRDKIIYGNLESAKLYVHVKPALGGTFTDIA+W
Sbjct: 311 AIHVCGTNLPAGGRNDTVCWMDLPTDGCRDKIIYGNLESAKLYVHVKPALGGTFTDIAMW 370

Query: 396 IFCPFNGPATLKLGIMNISLGKIGQHVGDWEHFTLRICNFTGQLYSIYFSQHSGGEWVDP 455
           +FCPFNGP+TLKLGI+NISLGKIGQHVGDWEH TLRICNF+G+L+SIYFSQHSGGEWVD 
Sbjct: 371 VFCPFNGPSTLKLGIVNISLGKIGQHVGDWEHITLRICNFSGELFSIYFSQHSGGEWVDA 430

Query: 456 YNLEFIEGNKATVYSSKSGHASYAHPGVYIQGSAKLGIGIRNDCARSHFLIDSSIHYEIV 515
           YNLEFIEGNKA VYSSKSGHASY HPG+YIQGS+KLGIGIRNDCARSH  IDSSIHYEIV
Sbjct: 431 YNLEFIEGNKAIVYSSKSGHASYPHPGLYIQGSSKLGIGIRNDCARSHLFIDSSIHYEIV 490

Query: 516 AAEYLKSNDIVEPCWLQFMREWGPTIVYSSRTRLDKMIDRLPSTIRFSVANIFRKLPAEL 575
           AAE+L+ N+IVEPCWLQFMREWGPTIVYSSRT+LD  IDRLP  IR +VANIFRKLPAEL
Sbjct: 491 AAEHLRCNEIVEPCWLQFMREWGPTIVYSSRTKLDNFIDRLPLKIRLTVANIFRKLPAEL 550

Query: 576 FGEGGPTGPKEKDNWEGDERG 597
           FGE GPTGPKEK+NWEGDERG
Sbjct: 551 FGEVGPTGPKEKNNWEGDERG 571

BLAST of Spg014727 vs. ExPASy TrEMBL
Match: A0A6J1H7X4 (uncharacterized protein LOC111460961 OS=Cucurbita moschata OX=3662 GN=LOC111460961 PE=4 SV=1)

HSP 1 Score: 1067.4 bits (2759), Expect = 2.2e-308
Identity = 493/561 (87.88%), Postives = 516/561 (91.98%), Query Frame = 0

Query: 36  SNAHYLLPSDEPDHFSLPSPIPEWPQGGGFASGIASLGEIEVLKITQFVSIWGYNLTRRE 95
           SN H LLPSDEP HFSLPSP+PEWPQGGGF SG ASLGEIEVLKITQF SIWG+NLT RE
Sbjct: 11  SNTHDLLPSDEPYHFSLPSPVPEWPQGGGFDSGKASLGEIEVLKITQFASIWGFNLTHRE 70

Query: 96  NNGVTFYRPLRIPEGFHCLGHYCQPNDKPLHGYLLVAKEVDASVQESDHISNIVKLPALV 155
           NNGVTFYRPLRIPEGFHCLGH+CQ ND+PLHGYLLVA+EVDA  QE DH+SNIVKLPALV
Sbjct: 71  NNGVTFYRPLRIPEGFHCLGHFCQRNDQPLHGYLLVAREVDAYFQEGDHVSNIVKLPALV 130

Query: 156 EPLDYALIWSPDDGSEEKYSECAYIWLPQPPDGYKSMGYFVTNKLKKPELSEVRCVRADL 215
           +PLDY LIWSPDDG EE+YSECAYIWLPQPPDGYKSMGYFVTNKLKKPEL EVRCVRADL
Sbjct: 131 KPLDYTLIWSPDDGREEQYSECAYIWLPQPPDGYKSMGYFVTNKLKKPELGEVRCVRADL 190

Query: 216 TDRCETYRLMLNISFKCTNFLVQIWSTRACHRGMLGRGVPVGTFYCGSYKSAEKELPIAC 275
           TDRCETYRLMLNIS KCTNF VQIWSTRACHRGMLGRGVPVGTFY GS+K  EKELPIAC
Sbjct: 191 TDRCETYRLMLNISLKCTNFPVQIWSTRACHRGMLGRGVPVGTFYSGSHKVTEKELPIAC 250

Query: 276 LKNLDSTLSTMPNLDQIHALIRHYGPTLFFHPKEIYLPSSVSWFFENGVRLHRDGLSSGE 335
           LKNLDSTL TMPNLDQIHALI HYGPT FFHPKEIYLPSSVSWFFENGV LHRDG+SSGE
Sbjct: 251 LKNLDSTLHTMPNLDQIHALINHYGPTFFFHPKEIYLPSSVSWFFENGVLLHRDGMSSGE 310

Query: 336 AIHVCGTNLPVGGRNDKECWMDLPTDGCRDKIIYGNLESAKLYVHVKPALGGTFTDIALW 395
           AIHVCGTNLP GGR++  CWMDLP+D CRDKIIYGNLESAKLYVHVKPALGGTFTDIA+W
Sbjct: 311 AIHVCGTNLPGGGRHETVCWMDLPSDDCRDKIIYGNLESAKLYVHVKPALGGTFTDIAMW 370

Query: 396 IFCPFNGPATLKLGIMNISLGKIGQHVGDWEHFTLRICNFTGQLYSIYFSQHSGGEWVDP 455
           +FCPFNG ATLKLGIM+ISLGKIGQHVGDWEH TLRICNFTG+L SIYFSQHSGGEWVD 
Sbjct: 371 VFCPFNGSATLKLGIMDISLGKIGQHVGDWEHVTLRICNFTGELSSIYFSQHSGGEWVDA 430

Query: 456 YNLEFIEGNKATVYSSKSGHASYAHPGVYIQGSAKLGIGIRNDCARSHFLIDSSIHYEIV 515
           YNLEFI+GNKA VYSSKSGHASY HPGVYIQGSA LGIGIRNDCARSH  IDSS HYEIV
Sbjct: 431 YNLEFIQGNKAIVYSSKSGHASYPHPGVYIQGSAMLGIGIRNDCARSHLCIDSSSHYEIV 490

Query: 516 AAEYLKSNDIVEPCWLQFMREWGPTIVYSSRTRLDKMIDRLPSTIRFSVANIFRKLPAEL 575
           AAEYL+ N +VEPCWLQFMREWGPTI+YSSRT LDKMI+ LPS IRFSVANI  KLP EL
Sbjct: 491 AAEYLRDNGVVEPCWLQFMREWGPTIIYSSRTTLDKMINFLPSMIRFSVANIIGKLPGEL 550

Query: 576 FGEGGPTGPKEKDNWEGDERG 597
           FGEGGPTGPKEK+NWEGDERG
Sbjct: 551 FGEGGPTGPKEKNNWEGDERG 571

BLAST of Spg014727 vs. ExPASy TrEMBL
Match: A0A6J1KXV7 (uncharacterized protein LOC111498114 OS=Cucurbita maxima OX=3661 GN=LOC111498114 PE=4 SV=1)

HSP 1 Score: 1062.8 bits (2747), Expect = 5.4e-307
Identity = 491/561 (87.52%), Postives = 515/561 (91.80%), Query Frame = 0

Query: 36  SNAHYLLPSDEPDHFSLPSPIPEWPQGGGFASGIASLGEIEVLKITQFVSIWGYNLTRRE 95
           SN H LLPSDEP HFSLPSP+PEWPQGGGF SG ASLGEIEVLKITQF SIWG+NLT RE
Sbjct: 11  SNTHDLLPSDEPYHFSLPSPVPEWPQGGGFGSGKASLGEIEVLKITQFASIWGFNLTHRE 70

Query: 96  NNGVTFYRPLRIPEGFHCLGHYCQPNDKPLHGYLLVAKEVDASVQESDHISNIVKLPALV 155
           NNGVTFYRPLRI EGFHCLGH+CQ ND+PLHGYLLVA+EVDA  Q+ D +SNIVKLPALV
Sbjct: 71  NNGVTFYRPLRISEGFHCLGHFCQRNDQPLHGYLLVAREVDAYFQDGDRVSNIVKLPALV 130

Query: 156 EPLDYALIWSPDDGSEEKYSECAYIWLPQPPDGYKSMGYFVTNKLKKPELSEVRCVRADL 215
           +PLDY LIWSPDDG EE+YSECAYIWLPQPPDGYKSMGYFVTNKLKKPEL EVRCVRADL
Sbjct: 131 KPLDYTLIWSPDDGREEQYSECAYIWLPQPPDGYKSMGYFVTNKLKKPELGEVRCVRADL 190

Query: 216 TDRCETYRLMLNISFKCTNFLVQIWSTRACHRGMLGRGVPVGTFYCGSYKSAEKELPIAC 275
           TDRCETYRLMLNISFKCTNF VQIWSTRACHRGMLGRGVPVGTFY GS+K  EKELPIAC
Sbjct: 191 TDRCETYRLMLNISFKCTNFPVQIWSTRACHRGMLGRGVPVGTFYSGSHKVTEKELPIAC 250

Query: 276 LKNLDSTLSTMPNLDQIHALIRHYGPTLFFHPKEIYLPSSVSWFFENGVRLHRDGLSSGE 335
           LKNLDSTL TMPNLDQIHALI HYGPT FFHPKEIYLPSSVSWFFENGV LHRDG+SSGE
Sbjct: 251 LKNLDSTLHTMPNLDQIHALINHYGPTFFFHPKEIYLPSSVSWFFENGVLLHRDGMSSGE 310

Query: 336 AIHVCGTNLPVGGRNDKECWMDLPTDGCRDKIIYGNLESAKLYVHVKPALGGTFTDIALW 395
           AIHVCGTNLP GGRN+  CWMDLP+D CRDKIIYGNLESAKLYVHVKPALGGTFTDIA+W
Sbjct: 311 AIHVCGTNLPGGGRNETVCWMDLPSDDCRDKIIYGNLESAKLYVHVKPALGGTFTDIAMW 370

Query: 396 IFCPFNGPATLKLGIMNISLGKIGQHVGDWEHFTLRICNFTGQLYSIYFSQHSGGEWVDP 455
           +FCPFNG ATLKLGIM+ISLGKIGQHVGDWEH TLRICNFTG+L SIYFSQHSGGEWVD 
Sbjct: 371 VFCPFNGSATLKLGIMDISLGKIGQHVGDWEHVTLRICNFTGELGSIYFSQHSGGEWVDA 430

Query: 456 YNLEFIEGNKATVYSSKSGHASYAHPGVYIQGSAKLGIGIRNDCARSHFLIDSSIHYEIV 515
           YNLEFI+GNKA VYSSKSGHASY HPGVYIQGSA LGIGIRNDCARSH  IDSS HYEIV
Sbjct: 431 YNLEFIQGNKAIVYSSKSGHASYPHPGVYIQGSAMLGIGIRNDCARSHLCIDSSSHYEIV 490

Query: 516 AAEYLKSNDIVEPCWLQFMREWGPTIVYSSRTRLDKMIDRLPSTIRFSVANIFRKLPAEL 575
           AAE+L+ N +VEPCWLQFMREWGPTI+YSSRT LDKMI+ LPS IRFSVANI  KLP EL
Sbjct: 491 AAEHLRDNGVVEPCWLQFMREWGPTIIYSSRTTLDKMINFLPSMIRFSVANIIGKLPGEL 550

Query: 576 FGEGGPTGPKEKDNWEGDERG 597
           FGEGGPTGPKEK+NWEGDERG
Sbjct: 551 FGEGGPTGPKEKNNWEGDERG 571

BLAST of Spg014727 vs. ExPASy TrEMBL
Match: A0A6J1DWM1 (uncharacterized protein LOC111024181 OS=Momordica charantia OX=3673 GN=LOC111024181 PE=4 SV=1)

HSP 1 Score: 1061.6 bits (2744), Expect = 1.2e-306
Identity = 491/561 (87.52%), Postives = 517/561 (92.16%), Query Frame = 0

Query: 36  SNAHYLLPSDEPDHFSLPSPIPEWPQGGGFASGIASLGEIEVLKITQFVSIWGYNLTRRE 95
           SNAHYLLPS+EPDHFSLPSPIPEWPQGG FASG  SLGEIEVLKITQFVSIWG NLT R+
Sbjct: 10  SNAHYLLPSEEPDHFSLPSPIPEWPQGGRFASGTTSLGEIEVLKITQFVSIWGCNLTYRD 69

Query: 96  NNGVTFYRPLRIPEGFHCLGHYCQPNDKPLHGYLLVAKEVDASVQESDHISNIVKLPALV 155
           N+GVTFYRPLRIPEGFHCLGHYCQPND+PLHGYLLVA+EVDA  QESDHIS IVKLPALV
Sbjct: 70  NDGVTFYRPLRIPEGFHCLGHYCQPNDRPLHGYLLVAREVDAYFQESDHISKIVKLPALV 129

Query: 156 EPLDYALIWSPDDGSEEKYSECAYIWLPQPPDGYKSMGYFVTNKLKKPELSEVRCVRADL 215
           EPLDY LIWSPDDGSE+KYSECAYIWLPQPPDGYKSMGY VTNKLKKPEL  VRCVRADL
Sbjct: 130 EPLDYELIWSPDDGSEDKYSECAYIWLPQPPDGYKSMGYVVTNKLKKPELGAVRCVRADL 189

Query: 216 TDRCETYRLMLNISFKCTNFLVQIWSTRACHRGMLGRGVPVGTFYCGSYKSAEKELPIAC 275
           TDRCETYRLMLNI+ KC  FLVQIWSTR+C RGMLG+GVP+GTFYCGS+K  EKELPIAC
Sbjct: 190 TDRCETYRLMLNINSKCPKFLVQIWSTRSCQRGMLGKGVPIGTFYCGSHKGTEKELPIAC 249

Query: 276 LKNLDSTLSTMPNLDQIHALIRHYGPTLFFHPKEIYLPSSVSWFFENGVRLHRDGLSSGE 335
           LKNLDSTL TMPNLDQIHALI HYGPT+FFHPKEIYLPSSVSWFFENGV LHRDG+SSGE
Sbjct: 250 LKNLDSTLPTMPNLDQIHALINHYGPTVFFHPKEIYLPSSVSWFFENGVLLHRDGISSGE 309

Query: 336 AIHVCGTNLPVGGRNDKECWMDLPTDGCRDKIIYGNLESAKLYVHVKPALGGTFTDIALW 395
           AIHVCGTNLP GG ND+  WMD P D CRD II GNL SAKLYVHVKPALGGTFTDIA+W
Sbjct: 310 AIHVCGTNLPGGGGNDR-FWMDFPIDSCRDTIIRGNLASAKLYVHVKPALGGTFTDIAMW 369

Query: 396 IFCPFNGPATLKLGIMNISLGKIGQHVGDWEHFTLRICNFTGQLYSIYFSQHSGGEWVDP 455
           +FCPFNGPATLKLG++NISLGKIGQHVGDWEHFTLRICNFTG+L+SIYFSQHSGGEWVD 
Sbjct: 370 VFCPFNGPATLKLGMVNISLGKIGQHVGDWEHFTLRICNFTGELWSIYFSQHSGGEWVDA 429

Query: 456 YNLEFIEGNKATVYSSKSGHASYAHPGVYIQGSAKLGIGIRNDCARSHFLIDSSIHYEIV 515
           YNLEFI+GNKA VYSSKSGHASY HPGVYIQG A LGIGIRNDCARSH  I+SSIHYEIV
Sbjct: 430 YNLEFIQGNKAIVYSSKSGHASYPHPGVYIQGCATLGIGIRNDCARSHLFINSSIHYEIV 489

Query: 516 AAEYLKSNDIVEPCWLQFMREWGPTIVYSSRTRLDKMIDRLPSTIRFSVANIFRKLPAEL 575
           AAEYL  + IVEPCWLQFMREWGPTI+YSSRT LDKMI+RLP TIRFSVANI +KLPAEL
Sbjct: 490 AAEYLGGSGIVEPCWLQFMREWGPTILYSSRTMLDKMINRLPLTIRFSVANILKKLPAEL 549

Query: 576 FGEGGPTGPKEKDNWEGDERG 597
           FGEGGPTGPKEKDNWEGDERG
Sbjct: 550 FGEGGPTGPKEKDNWEGDERG 569

BLAST of Spg014727 vs. ExPASy TrEMBL
Match: A0A0A0KDB1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G088000 PE=4 SV=1)

HSP 1 Score: 1018.1 bits (2631), Expect = 1.5e-293
Identity = 468/535 (87.48%), Postives = 495/535 (92.52%), Query Frame = 0

Query: 62  GGGFASGIASLGEIEVLKITQFVSIWGYNLTRRENNGVTFYRPLRIPEGFHCLGHYCQPN 121
           GGGFASGIASLGEIEVLKITQFVSIWG NL+RR NNGVTFYRPLR+PEG+HCLGHYCQPN
Sbjct: 64  GGGFASGIASLGEIEVLKITQFVSIWGCNLSRRGNNGVTFYRPLRMPEGYHCLGHYCQPN 123

Query: 122 DKPLHGYLLVAKEVDASVQESDHISNIVKLPALVEPLDYALIWSPDDGSEEKYSECAYIW 181
           D+PLHGYLLVA+EVD   QESDHISNIVKLPALVEP+D+ LIWSPDDGSEEKY ECAYIW
Sbjct: 124 DRPLHGYLLVAREVDGYFQESDHISNIVKLPALVEPIDFTLIWSPDDGSEEKYGECAYIW 183

Query: 182 LPQPPDGYKSMGYFVTNKLKKPELSEVRCVRADLTDRCETYRLMLNISFKCTNFLVQIWS 241
           LPQPPDGYKSMGYFVTNKL+KP + EVRCVRADLTDRCETYRLM NIS KC NFLVQIWS
Sbjct: 184 LPQPPDGYKSMGYFVTNKLEKPVVGEVRCVRADLTDRCETYRLMFNISSKCKNFLVQIWS 243

Query: 242 TRACHRGMLGRGVPVGTFYCGSYKSAEKELPIACLKNLDSTLSTMPNLDQIHALIRHYGP 301
           TRACHRGMLGRGVPVGTF+CGSYK  EKELPIACLKNL+STL TMPN+DQIH+LI HYGP
Sbjct: 244 TRACHRGMLGRGVPVGTFHCGSYKGTEKELPIACLKNLNSTLPTMPNIDQIHSLINHYGP 303

Query: 302 TLFFHPKEIYLPSSVSWFFENGVRLHRDGLSSGEAIHVCGTNLPVGGRNDKECWMDLPTD 361
           T+FFHPKEIYLPSSVSWFFENGV LHRDG+SSGEAI VCGTNLP  GRND  CWMDLPTD
Sbjct: 304 TVFFHPKEIYLPSSVSWFFENGVLLHRDGMSSGEAILVCGTNLPTDGRNDTVCWMDLPTD 363

Query: 362 GCRDKIIYGNLESAKLYVHVKPALGGTFTDIALWIFCPFNGPATLKLGIMNISLGKIGQH 421
           GCRDKII GNLESAKLY HVKPALGGTFTDIA+W+FCPFNGP+TLKLGI+NISLGKIGQH
Sbjct: 364 GCRDKIINGNLESAKLYAHVKPALGGTFTDIAMWVFCPFNGPSTLKLGIVNISLGKIGQH 423

Query: 422 VGDWEHFTLRICNFTGQLYSIYFSQHSGGEWVDPYNLEFIEGNKATVYSSKSGHASYAHP 481
           VGDWEH TLRICNFTG+L+SIYFSQHSGGEWVD YNLEFIEGNKA VYSSKSGHASY  P
Sbjct: 424 VGDWEHITLRICNFTGELFSIYFSQHSGGEWVDAYNLEFIEGNKAIVYSSKSGHASYPRP 483

Query: 482 GVYIQGSAKLGIGIRNDCARSHFLIDSSIHYEIVAAEYLKSNDIVEPCWLQFMREWGPTI 541
           G+YIQGS+KLGIGIRNDCARSH  IDSS HYEIVAAE+L+ NDIVEP WLQFMREWGPTI
Sbjct: 484 GLYIQGSSKLGIGIRNDCARSHLFIDSSTHYEIVAAEHLRRNDIVEPGWLQFMREWGPTI 543

Query: 542 VYSSRTRLDKMIDRLPSTIRFSVANIFRKLPAELFGEGGPTGPKEKDNWEGDERG 597
           VYSSRT+LD  IDRLP  IRF VANIFRKLPAELFGE GPTGPKEK+NWEGDERG
Sbjct: 544 VYSSRTKLDNFIDRLPLKIRFPVANIFRKLPAELFGEVGPTGPKEKNNWEGDERG 598

BLAST of Spg014727 vs. TAIR 10
Match: AT3G04350.1 (Plant protein of unknown function (DUF946) )

HSP 1 Score: 688.0 bits (1774), Expect = 6.9e-198
Identity = 317/558 (56.81%), Postives = 408/558 (73.12%), Query Frame = 0

Query: 44  SDEPDHFSLPSPIPEWPQGGGFASGIASLGEIEVLKITQFVSIWGYNLTRRENNGVTFYR 103
           S EP  FSLP+P+P WPQG GFA+G  SLGEIEV+KIT+F  +W  + +  ++   TFYR
Sbjct: 20  SSEPKPFSLPAPLPSWPQGKGFATGRISLGEIEVVKITKFHRVWSSDSSHDKSKRATFYR 79

Query: 104 PLRIPEGFHCLGHYCQPNDKPLHGYLLVAKEVDASVQESDHISNIVKLPALVEPLDYALI 163
              IPEGFHCLGHYCQP D+PL GY+L A+   A         N    P L +P+ Y+L+
Sbjct: 80  ADDIPEGFHCLGHYCQPTDQPLRGYVLAARTSKA--------VNADDFPPLKKPVSYSLV 139

Query: 164 WSPDDGSEEKYSECAYIWLPQPPDGYKSMGYFVTNKLKKPELSEVRCVRADLTDRCETYR 223
           WS D  SE+      Y WLP PP GY++MG  VT++  +PE  EVRCVR DLT+ CET  
Sbjct: 140 WSAD--SEKNGG--GYFWLPNPPVGYRAMGVIVTHEPGEPETEEVRCVREDLTESCETSE 199

Query: 224 LMLNI-SFKCTN---FLVQIWSTRACHRGMLGRGVPVGTFYCGSYK-SAEKELP-IACLK 283
           ++L + S K +N       +WSTR C RGML +GV VG+F+C +Y  S+E+ +P I CLK
Sbjct: 200 MILEVGSSKKSNGSSSPFSVWSTRPCERGMLSQGVAVGSFFCCTYDLSSERTVPDIGCLK 259

Query: 284 NLDSTLSTMPNLDQIHALIRHYGPTLFFHPKEIYLPSSVSWFFENGVRLHRDGLSSGEAI 343
           NLD TL  MPNLDQ+HA+I H+GPT++FHP+E Y+PSSV WFF+NG  L+R G S G+ I
Sbjct: 260 NLDPTLHAMPNLDQVHAVIEHFGPTVYFHPEEAYMPSSVQWFFKNGALLYRSGKSEGQPI 319

Query: 344 HVCGTNLPVGGRNDKECWMDLPTD-GCRDKIIYGNLESAKLYVHVKPALGGTFTDIALWI 403
           +  G+NLP GG ND + W+DLP D   +  +  GNLES++LYVHVKPALGGTFTDI +WI
Sbjct: 320 NSTGSNLPAGGCNDMDFWIDLPEDEEAKSNLKKGNLESSELYVHVKPALGGTFTDIVMWI 379

Query: 404 FCPFNGPATLKLGIMNISLGKIGQHVGDWEHFTLRICNFTGQLYSIYFSQHSGGEWVDPY 463
           FCPFNGPATLK+G+  + + +IG+HVGDWEHFT RICNF+G+L+ ++FSQHSGG WVD  
Sbjct: 380 FCPFNGPATLKIGLFTLPMTRIGEHVGDWEHFTFRICNFSGELWQMFFSQHSGGGWVDAS 439

Query: 464 NLEFIEGNKATVYSSKSGHASYAHPGVYIQGSAKLGIGIRNDCARSHFLIDSSIHYEIVA 523
           ++EF++ NK  VYSSK GHAS+ HPG+Y+QGS+KLGIG+RND A+S +++DSS  Y IVA
Sbjct: 440 DIEFVKDNKPAVYSSKHGHASFPHPGMYLQGSSKLGIGVRNDVAKSKYIVDSSQRYVIVA 499

Query: 524 AEYLKSNDIVEPCWLQFMREWGPTIVYSSRTRLDKMIDRLPSTIRFSVANIFRKLPAELF 583
           AEYL    ++EPCWLQ+MREWGPTI Y S + ++K+++ LP  +RFS+ NI    P  L+
Sbjct: 500 AEYLGKGAVIEPCWLQYMREWGPTIAYDSGSEINKIMNLLPLVVRFSIENIVDLFPIALY 559

Query: 584 GEGGPTGPKEKDNWEGDE 595
           GE GPTGPKEKDNWEGDE
Sbjct: 560 GEEGPTGPKEKDNWEGDE 565

BLAST of Spg014727 vs. TAIR 10
Match: AT1G04090.1 (Plant protein of unknown function (DUF946) )

HSP 1 Score: 686.0 bits (1769), Expect = 2.6e-197
Identity = 327/565 (57.88%), Postives = 410/565 (72.57%), Query Frame = 0

Query: 41  LLPSDEPDHFSLPSPIPEWPQGGGFASGIASLGEIEVLKITQFVSIWGYNLTRRENNGVT 100
           L P  +P+ FSLPS IP WP G GF SG  +LG+++V+KIT F  IW Y  T ++ N ++
Sbjct: 15  LPPLKDPETFSLPSSIPHWPPGQGFGSGTINLGKLQVIKITDFEFIWRYRSTEKKKN-IS 74

Query: 101 FYRPL-RIPEGFHCLGHYCQPNDKPLHGYLLVAKEVDASVQESDHISNIVKLPALVEPLD 160
           FY+P   +P+ FHCLGHYCQ +  PL GY+L A+++  S+++       V+ PALVEP+D
Sbjct: 75  FYKPKGLLPKDFHCLGHYCQSDSHPLRGYVLAARDLVDSLEQ-------VEKPALVEPVD 134

Query: 161 YALIWSPDDGSEEK---YSECAYIWLPQPPDGYKSMGYFVTNKLKKPELSEVRCVRADLT 220
           + L+WS +D +E +    SEC Y WLPQPP+GY+S+G+ VT    KPEL+EVRCVRADLT
Sbjct: 135 FTLVWSSNDSAENECSSKSECGYFWLPQPPEGYRSIGFVVTKTSVKPELNEVRCVRADLT 194

Query: 221 DRCETYRLMLNISFKCTNFLVQIWSTRACHRGMLGRGVPVGTFYCGSYKSAEKE---LPI 280
           D CE + +++    +     + IW TR   RGM G+GV  GTF+C +   A +E   + I
Sbjct: 195 DICEPHNVIVTAVSESLGVPLFIWRTRPSDRGMWGKGVSAGTFFCRTRLVAAREDLGIGI 254

Query: 281 ACLKNLDSTLSTMPNLDQIHALIRHYGPTLFFHPKEIYLPSSVSWFFENGVRLHRDGLSS 340
           ACLKNLD +L  MPN+DQI ALI+HYGPTL FHP E YLPSSVSWFF+NG  L   G   
Sbjct: 255 ACLKNLDLSLHAMPNVDQIQALIQHYGPTLVFHPGETYLPSSVSWFFKNGAVLCEKGNPI 314

Query: 341 GEAIHVCGTNLPVGGRNDKECWMDLPTDG-CRDKIIYGNLESAKLYVHVKPALGGTFTDI 400
            E I   G+NLP GG NDK+ W+DLP D   RD +  GNLES+KLY+H+KPALGGTFTD+
Sbjct: 315 EEPIDENGSNLPQGGSNDKQFWIDLPCDDQQRDFVKRGNLESSKLYIHIKPALGGTFTDL 374

Query: 401 ALWIFCPFNGPATLKLGIMNISLGKIGQHVGDWEHFTLRICNFTGQLYSIYFSQHSGGEW 460
             WIFCPFNGPATLKLG+++ISL  IGQHV DWEHFTLRI NF+G+LYSIY SQHSGGEW
Sbjct: 375 VFWIFCPFNGPATLKLGLVDISLISIGQHVCDWEHFTLRISNFSGELYSIYLSQHSGGEW 434

Query: 461 VDPYNLEFIEG-NKATVYSSKSGHASYAHPGVYIQGSAKLGIGIRNDCARSHFLIDSSIH 520
           ++ Y+LE I G NKA VYSSK GHAS+   G Y+QGS  LGIGIRND ARS  L+DSS  
Sbjct: 435 IEAYDLEIIPGSNKAVVYSSKHGHASFPRAGTYLQGSTMLGIGIRNDTARSELLVDSSSR 494

Query: 521 YEIVAAEYLKSNDIV-EPCWLQFMREWGPTIVYSSRTRLDKMIDRLPSTIRFSVANIFRK 580
           YEI+AAEYL  N ++ EP WLQ+MREWGP +VY SR  ++++++R P T+R S+A + RK
Sbjct: 495 YEIIAAEYLSGNSVLAEPPWLQYMREWGPKVVYDSREEIERLVNRFPRTVRVSLATVLRK 554

Query: 581 LPAELFGEGGPTGPKEKDNWEGDER 596
           LP EL GE GPTGPKEK+NW GDER
Sbjct: 555 LPVELSGEEGPTGPKEKNNWYGDER 571

BLAST of Spg014727 vs. TAIR 10
Match: AT5G43950.1 (Plant protein of unknown function (DUF946) )

HSP 1 Score: 683.7 bits (1763), Expect = 1.3e-196
Identity = 327/561 (58.29%), Postives = 408/561 (72.73%), Query Frame = 0

Query: 43  PSDEPDHFSLPSPIPEWPQGGGFASGIASLGEIEVLKITQFVSIWGYNLTRRENNGVTFY 102
           P  EP+ FSLP+ +P+WP G GF  G  +LGE+EV +IT F  +W Y   R     V+FY
Sbjct: 17  PLKEPETFSLPASLPQWPSGQGFGLGRINLGELEVAEITSFEFVWRYCSRRDNKKSVSFY 76

Query: 103 RPLRIPEGFHCLGHYCQPNDKPLHGYLLVAKEVDASVQESDHISNIVKLPALVEPLDYAL 162
           +P ++PE FHCLGHYCQ +   L G+LLVA++V+ S +           PALV+PLDY L
Sbjct: 77  KPDKLPEDFHCLGHYCQSDSHLLRGFLLVARQVNKSSE-----------PALVQPLDYTL 136

Query: 163 IWSPDDGSEEKYSEC-AYIWLPQPPDGYKSMGYFVTNKLKKPELSEVRCVRADLTDRCET 222
           +WS +D SEE+ SE   Y WLPQPP GYK +GY VT    KPEL +VRCVRADLTD+CE 
Sbjct: 137 VWSSNDLSEERQSESYGYFWLPQPPQGYKPIGYLVTTSPAKPELDQVRCVRADLTDKCEA 196

Query: 223 YRLMLNISFKCTNFLVQIWSTRACHRGMLGRGVPVGTFYCGSYKSAEKEL-PIACLKNLD 282
           +++++       +  + IW TR   RGM G+GV  GTF+C +    E  L  IACLKNLD
Sbjct: 197 HKVIITAISDSLSIPMFIWKTRPSDRGMRGKGVSTGTFFCTTQSPEEDHLSTIACLKNLD 256

Query: 283 STLSTMPNLDQIHALIRHYGPTLFFHPKEIYLPSSVSWFFENGVRLHRDGLSS---GEAI 342
           S+L  MPN++QIHA+I+HYGP ++FHP E+YLPSSVSWFF+NG  L  +  SS    E I
Sbjct: 257 SSLHAMPNIEQIHAMIQHYGPRVYFHPNEVYLPSSVSWFFKNGALLCSNSNSSVINNEPI 316

Query: 343 HVCGTNLPVGGRNDKECWMDLPTDG--CRDKIIYGNLESAKLYVHVKPALGGTFTDIALW 402
              G+NLP GG NDK  W+DLP +    R+ I  G+LES+KLYVHVKPA GGTFTD+A W
Sbjct: 317 DETGSNLPHGGTNDKRYWIDLPINDQQRREFIKRGDLESSKLYVHVKPAFGGTFTDLAFW 376

Query: 403 IFCPFNGPATLKLGIMNISLGKIGQHVGDWEHFTLRICNFTGQLYSIYFSQHSGGEWVDP 462
           IFCPFNGPATLKLG+M++SL K GQHV DWEHFT+RI NF+G+LYSIYFSQHSGGEW+ P
Sbjct: 377 IFCPFNGPATLKLGLMDLSLAKTGQHVCDWEHFTVRISNFSGELYSIYFSQHSGGEWIKP 436

Query: 463 YNLEFIEG-NKATVYSSKSGHASYAHPGVYIQGSAKLGIGIRNDCARSHFLIDSSIHYEI 522
            NLEF+EG NKA VYSSK+GHAS++  G+Y+QGSA LGIGIRND A+S   +DSS+ YEI
Sbjct: 437 ENLEFVEGSNKAVVYSSKNGHASFSKSGMYLQGSALLGIGIRNDSAKSDLFVDSSLKYEI 496

Query: 523 VAAEYLKSNDIVEPCWLQFMREWGPTIVYSSRTRLDKMIDRLPSTIRFSVANIFRKLPAE 582
           VAAEYL+   +VEP WL +MREWGP IVY+SR+ ++K+ +RLP  +R  V  + RK+P E
Sbjct: 497 VAAEYLR-GAVVEPPWLGYMREWGPKIVYNSRSEIEKLNERLPWRLRSWVDAVLRKIPVE 556

Query: 583 LFGEGGPTGPKEKDNWEGDER 596
           L GE GPTGPKEK+NW GDER
Sbjct: 557 LSGEEGPTGPKEKNNWFGDER 565

BLAST of Spg014727 vs. TAIR 10
Match: AT5G18490.1 (Plant protein of unknown function (DUF946) )

HSP 1 Score: 660.2 bits (1702), Expect = 1.6e-189
Identity = 307/556 (55.22%), Postives = 393/556 (70.68%), Query Frame = 0

Query: 44  SDEPDHFSLPSPIPEWPQGGGFASGIASLGEIEVLKITQFVSIWGYNLTRRENNGVTFYR 103
           S E   FSLPSP+P+WPQG GFA+G  SLGEI+V+K+T+F  +W    +R +    +FY+
Sbjct: 19  SSESKPFSLPSPLPQWPQGRGFATGRISLGEIQVVKVTEFDRVWKCGTSRGKLRCASFYK 78

Query: 104 PLRIPEGFHCLGHYCQPNDKPLHGYLLVAKEVDASVQESDHISNIVKLPALVEPLDYALI 163
           P+ IPEGFHCLGHYCQPN++PL G++L A+         DH       P L +PL+Y+L+
Sbjct: 79  PVGIPEGFHCLGHYCQPNNQPLRGFVLAARANKPGHLADDH------RPPLKKPLNYSLV 138

Query: 164 WSPDDGSEEKYSECAYIWLPQPPDGYKSMGYFVTNKLKKPELSEVRCVRADLTDRCETYR 223
           WS D       S+C Y WLP PP GY+++G  VT+  ++PE+ EVRCVR DLT+ CET  
Sbjct: 139 WSSD-------SDC-YFWLPNPPVGYRAVGVIVTDGSEEPEVDEVRCVREDLTESCETGE 198

Query: 224 LMLNISFKCTNFLVQIWSTRACHRGMLGRGVPVGTFYCGS---YKSAEKELPIACLKNLD 283
            +L +          +WST+ C RG+  RGV VG+F C +       +  + IACLKNLD
Sbjct: 199 KVLGVG------SFNVWSTKPCERGIWSRGVEVGSFVCSTNDLSSDNKAAMNIACLKNLD 258

Query: 284 STLSTMPNLDQIHALIRHYGPTLFFHPKEIYLPSSVSWFFENGVRLHRDGLSSGEAIHVC 343
            +L  MPNLDQ+HALI HYGP ++FHP+E Y+PSSV WFF+NG  LHR G S GE I+  
Sbjct: 259 PSLQGMPNLDQVHALIHHYGPMVYFHPEETYMPSSVPWFFKNGALLHRFGKSQGEPINSA 318

Query: 344 GTNLPVGGRNDKECWMDLPTD-GCRDKIIYGNLESAKLYVHVKPALGGTFTDIALWIFCP 403
           G+NLP GG ND   W+DLP D   R  +  GN+ES++LYVHVKPALGG FTD+ +WIFCP
Sbjct: 319 GSNLPAGGENDGSFWIDLPEDEEVRSNLKKGNIESSELYVHVKPALGGIFTDVVMWIFCP 378

Query: 404 FNGPATLKLGIMNISLGKIGQHVGDWEHFTLRICNFTGQLYSIYFSQHSGGEWVDPYNLE 463
           FNGPATLK+G++ + + ++G+HVGDWEHFT RI NF G L  ++FSQHSGG WVD  +LE
Sbjct: 379 FNGPATLKIGLLTVPMNRLGEHVGDWEHFTFRISNFNGDLTQMFFSQHSGGGWVDVSDLE 438

Query: 464 FIEG-NKATVYSSKSGHASYAHPGVYIQGSAKLGIGIRNDCARSHFLIDSSIHYEIVAAE 523
           F++G NK  VYSSK GHAS+ HPG+Y+QG +KLGIG+RND A+S +++DSS  Y IVAAE
Sbjct: 439 FVKGSNKPVVYSSKHGHASFPHPGMYLQGPSKLGIGVRNDVAKSKYMVDSSQRYRIVAAE 498

Query: 524 YLKSNDIVEPCWLQFMREWGPTIVYSSRTRLDKMIDRLPSTIRFSVANIFRKLPAELFGE 583
           YL    + EP WLQFMREWGPTIVY S   ++K+ID LP  +R S  ++F   P EL+GE
Sbjct: 499 YLGEGAVSEPYWLQFMREWGPTIVYDSAAEINKIIDLLPLILRNSFESLF---PIELYGE 551

Query: 584 GGPTGPKEKDNWEGDE 595
            GPTGPKEKDNWEGDE
Sbjct: 559 EGPTGPKEKDNWEGDE 551

BLAST of Spg014727 vs. TAIR 10
Match: AT2G44230.1 (Plant protein of unknown function (DUF946) )

HSP 1 Score: 471.1 bits (1211), Expect = 1.3e-132
Identity = 247/570 (43.33%), Postives = 340/570 (59.65%), Query Frame = 0

Query: 35  SSNAHYLLPSDEPDH-FSLPSPIPEWPQGGGFASGIASLGEIEVLKITQFVSIWGYNLTR 94
           +S+A    PS   D  F+LPSP+P WP G GFA G   LG +EV ++  F  +W      
Sbjct: 4   NSSAQSSTPSLPIDSTFNLPSPLPSWPSGEGFAKGRIDLGGLEVSQVDTFNKVWTVYEGG 63

Query: 95  RENNGVTFYRPLRIPEGFHCLGHYCQPNDKPLHGYLLVAKEVDASVQESDHISNIVKLPA 154
           ++N G TF+ P  +PEGF  LG Y QPN++ L G+ LV K++                 +
Sbjct: 64  QDNLGATFFEPSSVPEGFSILGFYAQPNNRKLFGWTLVGKDLSGD--------------S 123

Query: 155 LVEPLDYALIWSPDDGS-EEKYSECAYIWLPQPPDGYKSMGYFVTNKLKKPELSEVRCVR 214
           L  P+DY L+WS      E    E  Y W P PPDGY ++G  VT   +KP L ++RCVR
Sbjct: 124 LRPPVDYLLLWSGKSTKVENNKVETGYFWQPVPPDGYNAVGLIVTTSDEKPPLDKIRCVR 183

Query: 215 ADLTDRCETYRLMLNISFKCTNFLVQIWSTRACHRGMLGRGVPVGTFYCGSYKSAEKELP 274
           +DLTD+ E   L+    ++   F V   S++  +RG    GV VGTF+  S   A     
Sbjct: 184 SDLTDQSEPDALI----WETNGFSVS--SSKPVNRGTQASGVSVGTFFSNSPNPA----- 243

Query: 275 IACLKNLDSTLSTMPNLDQIHALIRHYGPTLFFHPKEIYLPSSVSWFFENGVRLHRDGLS 334
           + CLKN +   S MP+  QI AL + Y P ++FH  E YLPSSV+WFF NG  L++ G  
Sbjct: 244 LPCLKNNNFDFSCMPSKPQIDALFQTYAPWIYFHKDEKYLPSSVNWFFSNGALLYKKGDE 303

Query: 335 SGEA-IHVCGTNLPVGGRNDKECWMDLP-TDGCRDKIIYGNLESAKLYVHVKPALGGTFT 394
           S    +   G NLP G  ND   W+DLP     R ++  G+L+S ++Y+H+KP  GGTFT
Sbjct: 304 SNPVPVEPNGLNLPQGEFNDGLYWLDLPVASDARKRVQCGDLQSMEVYLHIKPVFGGTFT 363

Query: 395 DIALWIFCPFNGPATLKLGIMNISLGKIGQHVGDWEHFTLRICNFTGQLYSIYFSQHSGG 454
           DIA+W+F PFNGP+  KL   +I LG+IG+H+GDWEHFTLRI NF+G+L+ +Y SQHSGG
Sbjct: 364 DIAVWMFYPFNGPSRAKLKAASIPLGRIGEHIGDWEHFTLRISNFSGKLHRMYLSQHSGG 423

Query: 455 EWVDPYNLEFI-EGNKATVYSSKSGHASYAHPGVYIQGSAKLGIGIRNDCARSHFLIDSS 514
            W D   +EF   GNK   Y+S +GHA Y+ PG+ +QG  K  +GIRND  +S  +ID++
Sbjct: 424 SWADASEIEFQGGGNKPVAYASLNGHAMYSKPGLVLQG--KDNVGIRNDTGKSEKVIDTA 483

Query: 515 IHYEIVAAEYLKSNDIVEPCWLQFMREWGPTIVYSSRTRL---DKMI--DRLPSTIRFSV 574
           + + +VAAEY++  ++ EP WL +MR WGP I Y     +   +K++  + L +T R ++
Sbjct: 484 VRFRVVAAEYMR-GELEEPAWLNYMRHWGPKIDYGHENEIRGVEKIMVGESLKTTFRSAI 541

Query: 575 ANIFRKLPAELFGEGGPTGPKEKDNWEGDE 595
               + LP E+FGE GPTGPK K NW GDE
Sbjct: 544 ----KGLPNEVFGEEGPTGPKLKRNWLGDE 541

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038876230.10.0e+0086.37uncharacterized protein LOC120068509 isoform X1 [Benincasa hispida] >XP_03887623... [more]
XP_038876233.10.0e+0089.13uncharacterized protein LOC120068509 isoform X2 [Benincasa hispida][more]
XP_008459966.10.0e+0088.41PREDICTED: uncharacterized protein LOC103498924 [Cucumis melo] >XP_008459967.1 P... [more]
XP_004140668.14.6e-31087.88uncharacterized protein LOC101209282 isoform X2 [Cucumis sativus] >XP_011656767.... [more]
XP_022960118.14.5e-30887.88uncharacterized protein LOC111460961 [Cucurbita moschata] >XP_022960119.1 unchar... [more]
Match NameE-valueIdentityDescription
P532852.7e-0523.92Vacuolar protein sorting-associated protein 62 OS=Saccharomyces cerevisiae (stra... [more]
Match NameE-valueIdentityDescription
A0A1S3CBI00.0e+0088.41uncharacterized protein LOC103498924 OS=Cucumis melo OX=3656 GN=LOC103498924 PE=... [more]
A0A6J1H7X42.2e-30887.88uncharacterized protein LOC111460961 OS=Cucurbita moschata OX=3662 GN=LOC1114609... [more]
A0A6J1KXV75.4e-30787.52uncharacterized protein LOC111498114 OS=Cucurbita maxima OX=3661 GN=LOC111498114... [more]
A0A6J1DWM11.2e-30687.52uncharacterized protein LOC111024181 OS=Momordica charantia OX=3673 GN=LOC111024... [more]
A0A0A0KDB11.5e-29387.48Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G088000 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT3G04350.16.9e-19856.81Plant protein of unknown function (DUF946) [more]
AT1G04090.12.6e-19757.88Plant protein of unknown function (DUF946) [more]
AT5G43950.11.3e-19658.29Plant protein of unknown function (DUF946) [more]
AT5G18490.11.6e-18955.22Plant protein of unknown function (DUF946) [more]
AT2G44230.11.3e-13243.33Plant protein of unknown function (DUF946) [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR009291Vacuolar protein sorting-associated protein 62PFAMPF06101Vps62coord: 47..594
e-value: 3.0E-255
score: 847.7
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 577..596
NoneNo IPR availablePANTHERPTHR48173FAMILY NOT NAMEDcoord: 40..596
NoneNo IPR availablePANTHERPTHR48173:SF1PROCESSING PROTEIN PRP39, PUTATIVE-RELATEDcoord: 40..596

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg014727.1Spg014727.1mRNA