Spg014700 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg014700
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionrRNA N-glycosidase
Locationscaffold3: 36807816 .. 36808858 (-)
RNA-Seq ExpressionSpg014700
SyntenySpg014700
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAAAACAAGATGAAACTAATTGTTCTGTGCATTGTCTTGGCTGCTTCTCTCGCCAGCATCCACGATGTCTCGGCGGCTCGTACCATTGTCCTCAGCAAAACCACGCATCTCGTTGGTCGAGACGGGCTGTGCTTGGACGTGATCGGAGGGTACAACGACGACCATGTTCCAACTCAACTATGGCCATGTGGTACTCAAAACAACCAGCTATGGACCATTCAAACCGACGGGACGATTCGGTCCATGGGCAAATGCTTGGTCCCAAATGGCCATGACCCGGGCTCTTACACCATGATAAACGACTGCAATAAGGCCGATCCAAGCGACAAGACATGGAAACTATACCCCGATGGCACCCTCACCCACGTCAGCTCCGGCCTCGTCTTGACGGCTCAAGGAACCGGGTCGTCGTCGTCGTCGCACCTCATAACAACAATAGAGAGCAACACGTACGCTGCGATGCAAAGCTGGAGTACAGCGGACGACGGAGCACCTACCGTGGCTGCCATCGTAGGGCTCCGCCGCATGTGCATGCAGGCGCAGAATGACAACGTGCACGTTTGGATGAACGTGTGCATAAAAAACAATCGACAGCAATATTGGGCAATGTATGGCGACGGCACCATTCGAGCGAACTATAATACGAGCTTTTGCTTGAGTTCTACGGGCAAAGGCTCGAACGATGCTATTGTTTTAGCCAAGTGCCAGGGGTTGGCGCAGCAGCGGTGGGTGGCTAAGGAGGACGGGACCATCTTAAATCCCAGCACTGATTTGGTGATGGATGTGAAAGGAAGTGATCCCAGCCTTCGCCAGATTATTCTGTTTGCTCGTACTGGTAATCCTAACCAGCAGTGGGTGTTGATTACTCAGTATAAACCCTAAATAAAAATATGTGGATCTAGTTTTATGTATAATGCGTGCATGGCATGCATGCTTTTAAGTTACTATGGTATATTTAGTATTGCTTACAAATAATCAATAAAGAAGTGTGGCTTTTGTCATGTTTTTTTAAAGTTAATAAAAAATGGTTTTTGGTTCCC

mRNA sequence

ATGGAAAACAAGATGAAACTAATTGTTCTGTGCATTGTCTTGGCTGCTTCTCTCGCCAGCATCCACGATGTCTCGGCGGCTCGTACCATTGTCCTCAGCAAAACCACGCATCTCGTTGGTCGAGACGGGCTGTGCTTGGACGTGATCGGAGGGTACAACGACGACCATGTTCCAACTCAACTATGGCCATGTGGTACTCAAAACAACCAGCTATGGACCATTCAAACCGACGGGACGATTCGGTCCATGGGCAAATGCTTGGTCCCAAATGGCCATGACCCGGGCTCTTACACCATGATAAACGACTGCAATAAGGCCGATCCAAGCGACAAGACATGGAAACTATACCCCGATGGCACCCTCACCCACGTCAGCTCCGGCCTCGTCTTGACGGCTCAAGGAACCGGGTCGTCGTCGTCGTCGCACCTCATAACAACAATAGAGAGCAACACGTACGCTGCGATGCAAAGCTGGAGTACAGCGGACGACGGAGCACCTACCGTGGCTGCCATCGTAGGGCTCCGCCGCATGTGCATGCAGGCGCAGAATGACAACGTGCACGTTTGGATGAACGTGTGCATAAAAAACAATCGACAGCAATATTGGGCAATGTATGGCGACGGCACCATTCGAGCGAACTATAATACGAGCTTTTGCTTGAGTTCTACGGGCAAAGGCTCGAACGATGCTATTGTTTTAGCCAAGTGCCAGGGGTTGGCGCAGCAGCGGTGGGTGGCTAAGGAGGACGGGACCATCTTAAATCCCAGCACTGATTTGGTGATGGATGTGAAAGGAAGTGATCCCAGCCTTCGCCAGATTATTCTGTTTGCTCGTACTGGTAATCCTAACCAGCAGTGGGTGTTGATTACTCAGTATAAACCCTAA

Coding sequence (CDS)

ATGGAAAACAAGATGAAACTAATTGTTCTGTGCATTGTCTTGGCTGCTTCTCTCGCCAGCATCCACGATGTCTCGGCGGCTCGTACCATTGTCCTCAGCAAAACCACGCATCTCGTTGGTCGAGACGGGCTGTGCTTGGACGTGATCGGAGGGTACAACGACGACCATGTTCCAACTCAACTATGGCCATGTGGTACTCAAAACAACCAGCTATGGACCATTCAAACCGACGGGACGATTCGGTCCATGGGCAAATGCTTGGTCCCAAATGGCCATGACCCGGGCTCTTACACCATGATAAACGACTGCAATAAGGCCGATCCAAGCGACAAGACATGGAAACTATACCCCGATGGCACCCTCACCCACGTCAGCTCCGGCCTCGTCTTGACGGCTCAAGGAACCGGGTCGTCGTCGTCGTCGCACCTCATAACAACAATAGAGAGCAACACGTACGCTGCGATGCAAAGCTGGAGTACAGCGGACGACGGAGCACCTACCGTGGCTGCCATCGTAGGGCTCCGCCGCATGTGCATGCAGGCGCAGAATGACAACGTGCACGTTTGGATGAACGTGTGCATAAAAAACAATCGACAGCAATATTGGGCAATGTATGGCGACGGCACCATTCGAGCGAACTATAATACGAGCTTTTGCTTGAGTTCTACGGGCAAAGGCTCGAACGATGCTATTGTTTTAGCCAAGTGCCAGGGGTTGGCGCAGCAGCGGTGGGTGGCTAAGGAGGACGGGACCATCTTAAATCCCAGCACTGATTTGGTGATGGATGTGAAAGGAAGTGATCCCAGCCTTCGCCAGATTATTCTGTTTGCTCGTACTGGTAATCCTAACCAGCAGTGGGTGTTGATTACTCAGTATAAACCCTAA

Protein sequence

MENKMKLIVLCIVLAASLASIHDVSAARTIVLSKTTHLVGRDGLCLDVIGGYNDDHVPTQLWPCGTQNNQLWTIQTDGTIRSMGKCLVPNGHDPGSYTMINDCNKADPSDKTWKLYPDGTLTHVSSGLVLTAQGTGSSSSSHLITTIESNTYAAMQSWSTADDGAPTVAAIVGLRRMCMQAQNDNVHVWMNVCIKNNRQQYWAMYGDGTIRANYNTSFCLSSTGKGSNDAIVLAKCQGLAQQRWVAKEDGTILNPSTDLVMDVKGSDPSLRQIILFARTGNPNQQWVLITQYKP
Homology
BLAST of Spg014700 vs. NCBI nr
Match: XP_016903609.1 (PREDICTED: nigrin b-like [Cucumis melo])

HSP 1 Score: 497.7 bits (1280), Expect = 7.1e-137
Identity = 233/288 (80.90%), Postives = 262/288 (90.97%), Query Frame = 0

Query: 7   LIVLCIVLAASLASIHDVSAARTIVLSKTTHLVGRDGLCLDVIGGYNDDHVPTQLWPCGT 66
           +I +C+VLAASL  IH+VSAAR IVLS++THLVG+DGLCLDVIGGY+D+HVPTQLWPCG 
Sbjct: 8   IIFVCVVLAASLTGIHNVSAARAIVLSRSTHLVGQDGLCLDVIGGYSDNHVPTQLWPCGP 67

Query: 67  QNNQLWTIQTDGTIRSMGKCLVPNGHDPGSYTMINDCNKADPSDKTWKLYPDGTLTHVSS 126
           QNNQLWTIQ DGTIR+MGKCLVPNGHDPGSYTMI+DCNKADP+DKTWKLYPDGTLTHV S
Sbjct: 68  QNNQLWTIQADGTIRTMGKCLVPNGHDPGSYTMIDDCNKADPNDKTWKLYPDGTLTHVRS 127

Query: 127 GLVLTAQGTGSSSSSHLITTIESNTYAAMQSWSTADDGAPTVAAIVGLRRMCMQAQNDNV 186
            LVLT+QGTG    ++ ITTIE+NT A  QSW TADDG+P V AIVGLRRMC+QAQNDN+
Sbjct: 128 SLVLTSQGTG----AYAITTIETNTSAPTQSWGTADDGSPIVVAIVGLRRMCLQAQNDNI 187

Query: 187 HVWMNVCIKNNRQQYWAMYGDGTIRANYNTSFCLSSTGKGSNDAIVLAKCQGLAQQRWVA 246
           HVWMN C+KNNRQQYWA++ DGTIRANYNTSFCLSSTGKGSNDAIVLAKC+GL QQRWVA
Sbjct: 188 HVWMNSCVKNNRQQYWAVFSDGTIRANYNTSFCLSSTGKGSNDAIVLAKCEGLPQQRWVA 247

Query: 247 KEDGTILNPSTDLVMDVKGSDPSLRQIILFARTGNPNQQWVLITQYKP 295
           KEDGTIL+ ST+LVMDVKGSDP+LR+IILFA TGNPNQ W+ +TQYKP
Sbjct: 248 KEDGTILSLSTNLVMDVKGSDPNLRKIILFAPTGNPNQHWMFVTQYKP 291

BLAST of Spg014700 vs. NCBI nr
Match: KAA0059853.1 (nigrin b-like [Cucumis melo var. makuwa] >TYK24807.1 nigrin b-like [Cucumis melo var. makuwa])

HSP 1 Score: 497.7 bits (1280), Expect = 7.1e-137
Identity = 233/288 (80.90%), Postives = 262/288 (90.97%), Query Frame = 0

Query: 7   LIVLCIVLAASLASIHDVSAARTIVLSKTTHLVGRDGLCLDVIGGYNDDHVPTQLWPCGT 66
           +I +C+VLAASL  IH+VSAAR IVLS++THLVG+DGLCLDVIGGY+D+HVPTQLWPCG 
Sbjct: 13  IIFVCVVLAASLTGIHNVSAARAIVLSRSTHLVGQDGLCLDVIGGYSDNHVPTQLWPCGP 72

Query: 67  QNNQLWTIQTDGTIRSMGKCLVPNGHDPGSYTMINDCNKADPSDKTWKLYPDGTLTHVSS 126
           QNNQLWTIQ DGTIR+MGKCLVPNGHDPGSYTMI+DCNKADP+DKTWKLYPDGTLTHV S
Sbjct: 73  QNNQLWTIQADGTIRTMGKCLVPNGHDPGSYTMIDDCNKADPNDKTWKLYPDGTLTHVRS 132

Query: 127 GLVLTAQGTGSSSSSHLITTIESNTYAAMQSWSTADDGAPTVAAIVGLRRMCMQAQNDNV 186
            LVLT+QGTG    ++ ITTIE+NT A  QSW TADDG+P V AIVGLRRMC+QAQNDN+
Sbjct: 133 SLVLTSQGTG----AYAITTIETNTSAPTQSWGTADDGSPIVVAIVGLRRMCLQAQNDNI 192

Query: 187 HVWMNVCIKNNRQQYWAMYGDGTIRANYNTSFCLSSTGKGSNDAIVLAKCQGLAQQRWVA 246
           HVWMN C+KNNRQQYWA++ DGTIRANYNTSFCLSSTGKGSNDAIVLAKC+GL QQRWVA
Sbjct: 193 HVWMNSCVKNNRQQYWAVFSDGTIRANYNTSFCLSSTGKGSNDAIVLAKCEGLPQQRWVA 252

Query: 247 KEDGTILNPSTDLVMDVKGSDPSLRQIILFARTGNPNQQWVLITQYKP 295
           KEDGTIL+ ST+LVMDVKGSDP+LR+IILFA TGNPNQ W+ +TQYKP
Sbjct: 253 KEDGTILSLSTNLVMDVKGSDPNLRKIILFAPTGNPNQHWMFVTQYKP 296

BLAST of Spg014700 vs. NCBI nr
Match: XP_038885951.1 (nigrin b-like [Benincasa hispida])

HSP 1 Score: 491.5 bits (1264), Expect = 5.1e-135
Identity = 232/291 (79.73%), Postives = 252/291 (86.60%), Query Frame = 0

Query: 4   KMKLIVLCIVLAASLASIHDVSAARTIVLSKTTHLVGRDGLCLDVIGGYNDDHVPTQLWP 63
           KM ++ L +VLAAS    HDVSAARTIVLS++THLVGRDGLCLDVIGGY+D H+PTQLWP
Sbjct: 5   KMTIVFLVVVLAASFTGNHDVSAARTIVLSRSTHLVGRDGLCLDVIGGYSDSHIPTQLWP 64

Query: 64  CGTQNNQLWTIQTDGTIRSMGKCLVPNGHDPGSYTMINDCNKADPSDKTWKLYPDGTLTH 123
           CG QNNQLWTIQ DGTIR+MGKCLVPNGH+PGSYTMI+DCNK D  DKTWKLYPDGTL H
Sbjct: 65  CGPQNNQLWTIQVDGTIRTMGKCLVPNGHEPGSYTMIDDCNKVDTQDKTWKLYPDGTLVH 124

Query: 124 VSSGLVLTAQGTGSSSSSHLITTIESNTYAAMQSWSTADDGAPTVAAIVGLRRMCMQAQN 183
           V SGLVLT QGTG     H ITTIE+NT A  QSW TADDG+P V AIVGLR MC+QAQN
Sbjct: 125 VWSGLVLTGQGTG----PHAITTIETNTSAPTQSWGTADDGSPIVVAIVGLRHMCLQAQN 184

Query: 184 DNVHVWMNVCIKNNRQQYWAMYGDGTIRANYNTSFCLSSTGKGSNDAIVLAKCQGLAQQR 243
           DNVHVWMN C+KNNRQQYWA+Y DGTIR NYNTSFCLSSTGKG NDAI LAKCQGL QQR
Sbjct: 185 DNVHVWMNSCVKNNRQQYWAVYNDGTIRTNYNTSFCLSSTGKGLNDAIGLAKCQGLPQQR 244

Query: 244 WVAKEDGTILNPSTDLVMDVKGSDPSLRQIILFARTGNPNQQWVLITQYKP 295
           WVAKEDGTILNP  ++VMDVKGSDP+LR+IILFA TGNPNQQWV +TQYKP
Sbjct: 245 WVAKEDGTILNPINNMVMDVKGSDPNLRKIILFAPTGNPNQQWVFVTQYKP 291

BLAST of Spg014700 vs. NCBI nr
Match: XP_022150387.1 (nigrin b-like [Momordica charantia] >XP_022156716.1 nigrin b-like [Momordica charantia])

HSP 1 Score: 480.7 bits (1236), Expect = 8.9e-132
Identity = 228/287 (79.44%), Postives = 250/287 (87.11%), Query Frame = 0

Query: 1   MENKMKLIVLCIVLAASLASIHDVSAARTIVLSKTTHLVGRDGLCLDVIGGYNDDHVPTQ 60
           ME KM  IVLC+VLAASL  IH VSAA TIV S++TH+VGRDGLCLDVIGGYND+H+PTQ
Sbjct: 1   MEKKMMTIVLCLVLAASLVGIHHVSAAHTIVFSRSTHIVGRDGLCLDVIGGYNDNHIPTQ 60

Query: 61  LWPCGTQNNQLWTIQTDGTIRSMGKCLVPNGHDPGSYTMINDCNKADPSDKTWKLYPDGT 120
           LW C  QN+QLWTIQTDGTIRSMGKCLVPNG  PGSYTMI+DCN A+P DKTWKLYPDGT
Sbjct: 61  LWTCNPQNHQLWTIQTDGTIRSMGKCLVPNGRSPGSYTMIDDCNDANPDDKTWKLYPDGT 120

Query: 121 LTHVSSGLVLTAQGTGSSSSSHLITTIESNTYAAMQSWSTADDGAPTVAAIVGLRRMCMQ 180
           L HV SGLVLTAQ TG     H ITTIE+NTYAAMQSW+T DDG P V  IVGLRRMC+Q
Sbjct: 121 LAHVRSGLVLTAQATG----PHTITTIETNTYAAMQSWTTGDDGTPIVTTIVGLRRMCLQ 180

Query: 181 AQNDNVHVWMNVCIKNNRQQYWAMYGDGTIRANYNTSFCLSSTGKGSNDAIVLAKCQGLA 240
           AQNDNVHV MN C+KNNRQQYWA+YGDGTIRAN+NTS C+S TGKGSNDAIVLAKC+G  
Sbjct: 181 AQNDNVHVSMNSCMKNNRQQYWALYGDGTIRANFNTSLCMSGTGKGSNDAIVLAKCEGSP 240

Query: 241 QQRWVAKEDGTILNPSTDLVMDVKGSDPSLRQIILFARTGNPNQQWV 288
           QQRW+AKEDGTILN +T+LVMDVKGSDP+LRQIILF RTGNPNQQW+
Sbjct: 241 QQRWLAKEDGTILNLNTNLVMDVKGSDPNLRQIILFGRTGNPNQQWI 283

BLAST of Spg014700 vs. NCBI nr
Match: KAG5543116.1 (hypothetical protein RHGRI_016012 [Rhododendron griersonianum])

HSP 1 Score: 284.3 bits (726), Expect = 1.2e-72
Identity = 136/254 (53.54%), Postives = 171/254 (67.32%), Query Frame = 0

Query: 35  TTHLVGRDGLCLDVIGGYNDDHVPTQLWPCGTQNNQLWTIQTDGTIRSMGKCLVPNGHDP 94
           +TH+ GRDGLC+DV    +DD  P QLWPCG Q+NQ WT QTDGTIRS+GKC+    + P
Sbjct: 307 STHISGRDGLCVDVRDEKDDDGNPIQLWPCGQQSNQQWTFQTDGTIRSLGKCMTTYSYSP 366

Query: 95  GSYTMINDCNKADPSDKTWKLYPDGTLTHVSSGLVLTAQGTGSSSSSHLITTIESNTYAA 154
           G Y MI +C  A      W L  DGT+T+  SGLVLTA      ++     T+E+N +AA
Sbjct: 367 GVYVMIFNCGTAVQDATKWSLSSDGTITNPRSGLVLTANQAAQGTT----LTVETNVHAA 426

Query: 155 MQSWS-TADDGAPTVAAIVGLRRMCMQAQNDNVHVWMNVCIKNNRQQYWAMYGDGTIRAN 214
            Q W    ++  PTV  I+GL  +CMQA NDN  VW+  C+ +++QQ WA+YGDGTIR N
Sbjct: 427 RQGWRVVGEEVNPTVTTIIGLNDLCMQANNDNSRVWLESCVDSSQQQQWALYGDGTIRVN 486

Query: 215 YNTSFCLSSTGKGSNDAIVLAKCQGLAQQRWVAKEDGTILNPSTDLVMDVKGSDPSLRQI 274
            N + CL+S G  S D I++ +CQG A QRWV K DGTILNP+T LVMDVK SD SL QI
Sbjct: 487 SNHNLCLTSDGHSSLDVIIILRCQGWADQRWVFKSDGTILNPNTKLVMDVKDSDVSLHQI 546

Query: 275 ILFARTGNPNQQWV 288
           IL+  TGNPNQ+W+
Sbjct: 547 ILYQPTGNPNQRWL 556

BLAST of Spg014700 vs. ExPASy Swiss-Prot
Match: P33183 (Nigrin b OS=Sambucus nigra OX=4202 PE=1 SV=2)

HSP 1 Score: 261.2 bits (666), Expect = 1.5e-68
Identity = 129/266 (48.50%), Postives = 171/266 (64.29%), Query Frame = 0

Query: 29  TIVLSKTTHLVGRDGLCLDVIGGYNDDHVPTQLWPCGTQNNQLWTIQTDGTIRSMGKCLV 88
           T+  S T ++VGRDGLC+DV  GY+ D  P QLWPCGTQ NQ WT  +D TIRSMGKC+ 
Sbjct: 303 TLRTSFTRNIVGRDGLCVDVRNGYDTDGTPLQLWPCGTQRNQRWTFDSDDTIRSMGKCMT 362

Query: 89  PNGHDPGSYTMINDCNKADPSDKTWKLYPDGTLTHVSSGLVLTAQGTGSSSSSHLITTIE 148
            NG + GS  +I +C+ A  +   W++  DG++ + SSGLV+TA      ++S  I  +E
Sbjct: 363 ANGLNNGSNIVIFNCSTAAENAIKWEVPIDGSIINPSSGLVMTA----PRAASRTILLLE 422

Query: 149 SNTYAAMQSWSTADDGAPTVAAIVGLRRMCMQAQNDNVHVWMNVCIKNNRQQYWAMYGDG 208
            N YAA Q W+  ++  P VA+IVG + MC+Q+  +N  VWM  C   + QQ WA+YGD 
Sbjct: 423 DNIYAASQGWTVTNNVKPIVASIVGYKEMCLQSNGENNGVWMEDCEATSLQQQWALYGDR 482

Query: 209 TIRANYNTSFCLSSTGKGSNDAIVLAKCQGLAQQRWVAKEDGTILNPSTDLVMDVKGSDP 268
           TIR N     C+++ G  S D I++ KCQGL  QRW    DG I+NP +  VMDV+ S+ 
Sbjct: 483 TIRVNSTRGLCVTTNGYNSKDLIIILKCQGLPSQRWFFNSDGAIVNPKSRHVMDVRASNV 542

Query: 269 SLRQIILFARTGNPNQQWVLITQYKP 295
           SLR+II+F  TGNPNQQWV  TQ  P
Sbjct: 543 SLREIIIFPATGNPNQQWV--TQVLP 562

BLAST of Spg014700 vs. ExPASy Swiss-Prot
Match: Q9M653 (Ribosome-inactivating protein PMRIPt OS=Polygonatum multiflorum OX=45371 GN=RIPt PE=1 SV=1)

HSP 1 Score: 253.1 bits (645), Expect = 4.0e-66
Identity = 128/263 (48.67%), Postives = 168/263 (63.88%), Query Frame = 0

Query: 35  TTHLVGRDGLCLDVIGGYNDDHVPTQLWPCGTQNNQLWTIQTDGTIRSMGKCLVPNGHDP 94
           T  + G DGLC+DV    N+D +P QLWPCG Q NQ WT  TDGTI+SMGKC+  NG+ P
Sbjct: 341 TRRISGLDGLCMDVRNESNNDGIPIQLWPCGAQRNQQWTFHTDGTIQSMGKCMTSNGYHP 400

Query: 95  GSYTMINDCNKADPSDKT-WKLYPDGTLTHVSSGLVLTAQGTGSSSSSHLITTIESNTYA 154
           G Y MI +C+ A   D T W +  DG++T+  SGLVLTA     ++    I  +  NT++
Sbjct: 401 GDYVMIFNCSTAPVPDATKWVVSIDGSITNPHSGLVLTAPQAAQTT----ILLVVRNTHS 460

Query: 155 AMQSWSTADDGAPTVAAIVGLRRMCMQAQND-NVHVWMNVCIKNNRQQYWAMYGDGTIRA 214
           A Q  S  DD  P V  IVG + MC+Q  N+ N  VW+  C  +  QQ+WA+Y DGTIR 
Sbjct: 461 AKQGRSVGDDVEPIVTYIVGFKYMCLQGNNENNTRVWLEDCAVDRPQQWWALYSDGTIRV 520

Query: 215 NYNTSFCLSSTGKGSNDAIVLAKCQGLAQQRWVAKEDGTILNPSTDLVMDVKGSDPSLRQ 274
           + + S C++S G  S DAI++  C G   QR V   DGTILNP+  LVMDV+ S+ +LRQ
Sbjct: 521 DSDRSLCVTSDGHSSRDAIIILTCDGGINQRLVFNTDGTILNPNAQLVMDVRQSNVALRQ 580

Query: 275 IILFARTGNPNQQWV-LITQYKP 295
           IIL+  TGNPNQQW+ +IT+ +P
Sbjct: 581 IILYQPTGNPNQQWMTMITRTRP 599

BLAST of Spg014700 vs. ExPASy Swiss-Prot
Match: Q41358 (Ribosome-inactivating protein SNAI OS=Sambucus nigra OX=4202 GN=SNA-I PE=1 SV=1)

HSP 1 Score: 234.6 bits (597), Expect = 1.5e-60
Identity = 122/260 (46.92%), Postives = 158/260 (60.77%), Query Frame = 0

Query: 31  VLSKTTHLVGRDGLCLDVIGGYNDDHVPTQLWPCGTQNNQLWTIQTDGTIRSMGKCLVPN 90
           V+  T  + G DGLC+DV  G+  D  P QL PCG + NQLWT +TDGTIR +GKCL  +
Sbjct: 318 VVEVTRRISGWDGLCVDVRYGHYIDGNPVQLRPCGNECNQLWTFRTDGTIRWLGKCLTAS 377

Query: 91  GHDPGSYTMINDCNKADPSDKTWKLYPDGTLTHVSSGLVLTAQGTGSSSSSHLITTIESN 150
                S  MI DCN   P    W +  DGT+T+  SGLVLTA      ++     ++E+N
Sbjct: 378 -----SSVMIYDCNTVPPEATKWVVSIDGTITNPHSGLVLTAPQAAEGTA----LSLENN 437

Query: 151 TYAAMQSWSTADDGAPTVAAIVGLRRMCMQAQNDNVHVWMNVCIKNNRQQYWAMYGDGTI 210
            +AA Q W T  D  P V  IVG ++MC++   +N  VW+  C+ N  QQ WA+YGDGTI
Sbjct: 438 IHAARQGW-TVGDVEPLVTFIVGYKQMCLRENGENNFVWLEDCVLNRVQQEWALYGDGTI 497

Query: 211 RANYNTSFCLSSTGKGSNDAIVLAKCQGLAQQRWVAKEDGTILNPSTDLVMDVKGSDPSL 270
           R N N S C++S     +D IV+ KC+G   QRWV   +GTI NP+  L+MDV   D SL
Sbjct: 498 RVNSNRSLCVTSEDHEPSDLIVILKCEGSGNQRWVFNTNGTISNPNAKLLMDVAQRDVSL 557

Query: 271 RQIILFARTGNPNQQWVLIT 291
           R+IIL+  TGNPNQQW+  T
Sbjct: 558 RKIILYRPTGNPNQQWITTT 567

BLAST of Spg014700 vs. ExPASy Swiss-Prot
Match: O22415 (Ribosome-inactivating protein SNAIf OS=Sambucus nigra OX=4202 GN=SNA-If PE=1 SV=1)

HSP 1 Score: 234.6 bits (597), Expect = 1.5e-60
Identity = 122/261 (46.74%), Postives = 158/261 (60.54%), Query Frame = 0

Query: 31  VLSKTTHLVGRDGLCLDVIGGYNDDHVPTQLWPCGTQNNQLWTIQTDGTIRSMGKCLVPN 90
           V+  T  + G DGLC+DV  G+  D    QL PCG + NQLWT +TDGTIR +GKCL  +
Sbjct: 318 VVEVTRRISGWDGLCVDVRDGHYIDGNTVQLGPCGNECNQLWTFRTDGTIRWLGKCLTTS 377

Query: 91  GHDPGSYTMINDCNKADPSDKTWKLYPDGTLTHVSSGLVLTAQGTGSSSSSHLITTIESN 150
                S  MI DCN   P    W +  DGT+T+  SGLVLTA      ++     ++E+N
Sbjct: 378 -----SSVMIYDCNTVPPEATKWVVSTDGTITNPRSGLVLTAPQAAEGTA----LSLENN 437

Query: 151 TYAAMQSWSTADDGAPTVAAIVGLRRMCMQAQNDNVHVWMNVCIKNNRQQYWAMYGDGTI 210
            +AA Q W T  D  P V  IVG ++MC+    +N  VW+  C+ N  +Q WA+YGDGTI
Sbjct: 438 IHAARQGW-TVGDVEPLVTFIVGYKQMCLTENGENNFVWLEDCVLNRVEQEWALYGDGTI 497

Query: 211 RANYNTSFCLSSTGKGSNDAIVLAKCQGLAQQRWVAKEDGTILNPSTDLVMDVKGSDPSL 270
           R N N S C++S     +D IV+ KC+G   QRWV   +GTI NP+  LVMDV  S+ SL
Sbjct: 498 RVNSNRSLCVTSEDHEPSDLIVILKCEGSGNQRWVFNTNGTISNPNAKLVMDVAQSNVSL 557

Query: 271 RQIILFARTGNPNQQWVLITQ 292
           R+IIL+  TGNPNQQW+  TQ
Sbjct: 558 RKIILYPPTGNPNQQWITTTQ 568

BLAST of Spg014700 vs. ExPASy Swiss-Prot
Match: P11140 (Abrin-a OS=Abrus precatorius OX=3816 PE=1 SV=2)

HSP 1 Score: 215.3 bits (547), Expect = 9.2e-55
Identity = 116/259 (44.79%), Postives = 159/259 (61.39%), Query Frame = 0

Query: 35  TTHLVGRDGLCLDVI-GGYNDDHVPTQLWPC--GTQNNQLWTIQTDGTIRSMGKCLVPNG 94
           T  + GRDG+C+DV   GY++ +    +W C    + NQLWT+++D TIRS GKCL   G
Sbjct: 276 TVRIGGRDGMCVDVYDNGYHNGN-RIIMWKCKDRLEENQLWTLKSDKTIRSNGKCLTTYG 335

Query: 95  HDPGSYTMINDCNKADPSDKTWKLYPDGTLTHVSSGLVLTAQGTGSSSSSHLITTIESNT 154
           + PGSY MI DC  A      W+++ +GT+ +  S LVL+A+    SSS     T+++N 
Sbjct: 336 YAPGSYVMIYDCTSAVAEATYWEIWDNGTIINPKSALVLSAE----SSSMGGTLTVQTNE 395

Query: 155 YAAMQSWSTADDGAPTVAAIVGLRRMCMQAQNDNVHVWMNVCIKNNRQQYWAMYGDGTIR 214
           Y   Q W T ++ +P V +I G   +CMQAQ  N  VWM  C  N ++Q WA+Y DG+IR
Sbjct: 396 YLMRQGWRTGNNTSPFVTSISGYSDLCMQAQGSN--VWMADCDSNKKEQQWALYTDGSIR 455

Query: 215 ANYNTSFCLSSTGKGSNDAIVLAKC-QGLAQQRWVAKEDGTILNPSTDLVMDVKGSDPSL 274
           +  NT+ CL+S        I+L  C  G A QRWV K DG+I +   D+VMDVKGSDPSL
Sbjct: 456 SVQNTNNCLTSKDHKQGSTILLMGCSNGWASQRWVFKNDGSIYSLYDDMVMDVKGSDPSL 515

Query: 275 RQIILFARTGNPNQQWVLI 290
           +QIIL+  TG PNQ W+ +
Sbjct: 516 KQIILWPYTGKPNQIWLTL 527

BLAST of Spg014700 vs. ExPASy TrEMBL
Match: A0A5A7UV90 (Nigrin b-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold184G00550 PE=3 SV=1)

HSP 1 Score: 497.7 bits (1280), Expect = 3.4e-137
Identity = 233/288 (80.90%), Postives = 262/288 (90.97%), Query Frame = 0

Query: 7   LIVLCIVLAASLASIHDVSAARTIVLSKTTHLVGRDGLCLDVIGGYNDDHVPTQLWPCGT 66
           +I +C+VLAASL  IH+VSAAR IVLS++THLVG+DGLCLDVIGGY+D+HVPTQLWPCG 
Sbjct: 13  IIFVCVVLAASLTGIHNVSAARAIVLSRSTHLVGQDGLCLDVIGGYSDNHVPTQLWPCGP 72

Query: 67  QNNQLWTIQTDGTIRSMGKCLVPNGHDPGSYTMINDCNKADPSDKTWKLYPDGTLTHVSS 126
           QNNQLWTIQ DGTIR+MGKCLVPNGHDPGSYTMI+DCNKADP+DKTWKLYPDGTLTHV S
Sbjct: 73  QNNQLWTIQADGTIRTMGKCLVPNGHDPGSYTMIDDCNKADPNDKTWKLYPDGTLTHVRS 132

Query: 127 GLVLTAQGTGSSSSSHLITTIESNTYAAMQSWSTADDGAPTVAAIVGLRRMCMQAQNDNV 186
            LVLT+QGTG    ++ ITTIE+NT A  QSW TADDG+P V AIVGLRRMC+QAQNDN+
Sbjct: 133 SLVLTSQGTG----AYAITTIETNTSAPTQSWGTADDGSPIVVAIVGLRRMCLQAQNDNI 192

Query: 187 HVWMNVCIKNNRQQYWAMYGDGTIRANYNTSFCLSSTGKGSNDAIVLAKCQGLAQQRWVA 246
           HVWMN C+KNNRQQYWA++ DGTIRANYNTSFCLSSTGKGSNDAIVLAKC+GL QQRWVA
Sbjct: 193 HVWMNSCVKNNRQQYWAVFSDGTIRANYNTSFCLSSTGKGSNDAIVLAKCEGLPQQRWVA 252

Query: 247 KEDGTILNPSTDLVMDVKGSDPSLRQIILFARTGNPNQQWVLITQYKP 295
           KEDGTIL+ ST+LVMDVKGSDP+LR+IILFA TGNPNQ W+ +TQYKP
Sbjct: 253 KEDGTILSLSTNLVMDVKGSDPNLRKIILFAPTGNPNQHWMFVTQYKP 296

BLAST of Spg014700 vs. ExPASy TrEMBL
Match: A0A1S4E5V9 (nigrin b-like OS=Cucumis melo OX=3656 GN=LOC107992255 PE=3 SV=1)

HSP 1 Score: 497.7 bits (1280), Expect = 3.4e-137
Identity = 233/288 (80.90%), Postives = 262/288 (90.97%), Query Frame = 0

Query: 7   LIVLCIVLAASLASIHDVSAARTIVLSKTTHLVGRDGLCLDVIGGYNDDHVPTQLWPCGT 66
           +I +C+VLAASL  IH+VSAAR IVLS++THLVG+DGLCLDVIGGY+D+HVPTQLWPCG 
Sbjct: 8   IIFVCVVLAASLTGIHNVSAARAIVLSRSTHLVGQDGLCLDVIGGYSDNHVPTQLWPCGP 67

Query: 67  QNNQLWTIQTDGTIRSMGKCLVPNGHDPGSYTMINDCNKADPSDKTWKLYPDGTLTHVSS 126
           QNNQLWTIQ DGTIR+MGKCLVPNGHDPGSYTMI+DCNKADP+DKTWKLYPDGTLTHV S
Sbjct: 68  QNNQLWTIQADGTIRTMGKCLVPNGHDPGSYTMIDDCNKADPNDKTWKLYPDGTLTHVRS 127

Query: 127 GLVLTAQGTGSSSSSHLITTIESNTYAAMQSWSTADDGAPTVAAIVGLRRMCMQAQNDNV 186
            LVLT+QGTG    ++ ITTIE+NT A  QSW TADDG+P V AIVGLRRMC+QAQNDN+
Sbjct: 128 SLVLTSQGTG----AYAITTIETNTSAPTQSWGTADDGSPIVVAIVGLRRMCLQAQNDNI 187

Query: 187 HVWMNVCIKNNRQQYWAMYGDGTIRANYNTSFCLSSTGKGSNDAIVLAKCQGLAQQRWVA 246
           HVWMN C+KNNRQQYWA++ DGTIRANYNTSFCLSSTGKGSNDAIVLAKC+GL QQRWVA
Sbjct: 188 HVWMNSCVKNNRQQYWAVFSDGTIRANYNTSFCLSSTGKGSNDAIVLAKCEGLPQQRWVA 247

Query: 247 KEDGTILNPSTDLVMDVKGSDPSLRQIILFARTGNPNQQWVLITQYKP 295
           KEDGTIL+ ST+LVMDVKGSDP+LR+IILFA TGNPNQ W+ +TQYKP
Sbjct: 248 KEDGTILSLSTNLVMDVKGSDPNLRKIILFAPTGNPNQHWMFVTQYKP 291

BLAST of Spg014700 vs. ExPASy TrEMBL
Match: A0A6J1DVT2 (nigrin b-like OS=Momordica charantia OX=3673 GN=LOC111023562 PE=3 SV=1)

HSP 1 Score: 480.7 bits (1236), Expect = 4.3e-132
Identity = 228/287 (79.44%), Postives = 250/287 (87.11%), Query Frame = 0

Query: 1   MENKMKLIVLCIVLAASLASIHDVSAARTIVLSKTTHLVGRDGLCLDVIGGYNDDHVPTQ 60
           ME KM  IVLC+VLAASL  IH VSAA TIV S++TH+VGRDGLCLDVIGGYND+H+PTQ
Sbjct: 1   MEKKMMTIVLCLVLAASLVGIHHVSAAHTIVFSRSTHIVGRDGLCLDVIGGYNDNHIPTQ 60

Query: 61  LWPCGTQNNQLWTIQTDGTIRSMGKCLVPNGHDPGSYTMINDCNKADPSDKTWKLYPDGT 120
           LW C  QN+QLWTIQTDGTIRSMGKCLVPNG  PGSYTMI+DCN A+P DKTWKLYPDGT
Sbjct: 61  LWTCNPQNHQLWTIQTDGTIRSMGKCLVPNGRSPGSYTMIDDCNDANPDDKTWKLYPDGT 120

Query: 121 LTHVSSGLVLTAQGTGSSSSSHLITTIESNTYAAMQSWSTADDGAPTVAAIVGLRRMCMQ 180
           L HV SGLVLTAQ TG     H ITTIE+NTYAAMQSW+T DDG P V  IVGLRRMC+Q
Sbjct: 121 LAHVRSGLVLTAQATG----PHTITTIETNTYAAMQSWTTGDDGTPIVTTIVGLRRMCLQ 180

Query: 181 AQNDNVHVWMNVCIKNNRQQYWAMYGDGTIRANYNTSFCLSSTGKGSNDAIVLAKCQGLA 240
           AQNDNVHV MN C+KNNRQQYWA+YGDGTIRAN+NTS C+S TGKGSNDAIVLAKC+G  
Sbjct: 181 AQNDNVHVSMNSCMKNNRQQYWALYGDGTIRANFNTSLCMSGTGKGSNDAIVLAKCEGSP 240

Query: 241 QQRWVAKEDGTILNPSTDLVMDVKGSDPSLRQIILFARTGNPNQQWV 288
           QQRW+AKEDGTILN +T+LVMDVKGSDP+LRQIILF RTGNPNQQW+
Sbjct: 241 QQRWLAKEDGTILNLNTNLVMDVKGSDPNLRQIILFGRTGNPNQQWI 283

BLAST of Spg014700 vs. ExPASy TrEMBL
Match: A0A6A4L4D0 (Uncharacterized protein (Fragment) OS=Rhododendron williamsianum OX=262921 GN=C3L33_15736 PE=4 SV=1)

HSP 1 Score: 279.3 bits (713), Expect = 1.9e-71
Identity = 132/258 (51.16%), Postives = 171/258 (66.28%), Query Frame = 0

Query: 31  VLSKTTHLVGRDGLCLDVIGGYNDDHVPTQLWPCG-TQNNQLWTIQTDGTIRSMGKCLVP 90
           +L+ T HL GRDG C+DV  G ++D  P QLWPCG  Q+NQ WT +TDGTIRS+GKC+  
Sbjct: 89  ILNSTRHLSGRDGFCMDVRDGRDNDGNPIQLWPCGQQQSNQQWTFRTDGTIRSLGKCMTA 148

Query: 91  NGHDPGSYTMINDCNKADPSDKTWKLYPDGTLTHVSSGLVLTAQGTGSSSSSHLITTIES 150
           +G+ PG Y MI DC  A      W L  DGT+T+  SGLVLTA       ++    T+E+
Sbjct: 149 SGYSPGHYVMIFDCETAGQDATKWSLSTDGTITNPHSGLVLTATNEHVQGNT---LTVET 208

Query: 151 NTYAAMQSWSTADDGAPTVAAIVGLRRMCMQAQNDNVHVWMNVCIKNNRQQYWAMYGDGT 210
           N +AA Q W   ++  PTV  I+G   +CMQA NDN  VW+  C+ + +QQ WA+YGDGT
Sbjct: 209 NIHAASQGWRVVEEVYPTVTTIMGFNDLCMQANNDNTRVWLESCVDSRQQQQWALYGDGT 268

Query: 211 IRANYNTSFCLSSTGKGSNDAIVLAKCQGLAQQRWVAKEDGTILNPSTDLVMDVKGSDPS 270
           IR N + + CL+S G  S D I++ +CQG   QRWV   DGTILNP+  LVMDV+ SD S
Sbjct: 269 IRVNSDHNLCLTSDGHSSLDVIIVRRCQGWGNQRWVFHTDGTILNPNAKLVMDVRQSDVS 328

Query: 271 LRQIILFARTGNPNQQWV 288
           L+QIIL+  TGNPNQ+W+
Sbjct: 329 LKQIILYQPTGNPNQRWL 343

BLAST of Spg014700 vs. ExPASy TrEMBL
Match: Q8GTA5 (Monomeric lectin SNAlm OS=Sambucus nigra OX=4202 PE=2 SV=1)

HSP 1 Score: 268.5 bits (685), Expect = 3.4e-68
Identity = 131/260 (50.38%), Postives = 172/260 (66.15%), Query Frame = 0

Query: 35  TTHLVGRDGLCLDVIGGYNDDHVPTQLWPCGTQNNQLWTIQTDGTIRSMGKCLVPNGHDP 94
           T ++VGRDGLC+DV  GY+ D  P QLWPCGTQ NQ WT  TD TIRSMGKC+  NG + 
Sbjct: 62  TGNIVGRDGLCVDVRNGYDTDGTPIQLWPCGTQRNQQWTFHTDDTIRSMGKCMTANGLNN 121

Query: 95  GSYTMINDCNKADPSDKTWKLYPDGTLTHVSSGLVLTAQGTGSSSSSHLITTIESNTYAA 154
           GS  MI +C+ A  +   W++  DG++ + SSGLV+TA     S++S  I  +++N YAA
Sbjct: 122 GSNIMIFNCSTAVENAIKWEVTIDGSIINPSSGLVITA----PSAASRTILLLQNNIYAA 181

Query: 155 MQSWSTADDGAPTVAAIVGLRRMCMQAQNDNVHVWMNVCIKNNRQQYWAMYGDGTIRANY 214
            Q W+ +++  P VA+IVG R MC+QA  +N  VWM  C   + QQ WA++GD TIR N 
Sbjct: 182 SQGWTVSNNVQPIVASIVGFREMCLQANGENNGVWMEDCEATSLQQQWALFGDRTIRVNS 241

Query: 215 NTSFCLSSTGKGSNDAIVLAKCQGLAQQRWVAKEDGTILNPSTDLVMDVKGSDPSLRQII 274
           N   C+++ G  S D I++ KCQGL  QRW    DG I+NP + LVMDVK S+ SLR+II
Sbjct: 242 NRGLCVTTNGYNSRDLIIILKCQGLPSQRWFFNSDGAIVNPKSKLVMDVKSSNVSLREII 301

Query: 275 LFARTGNPNQQWVLITQYKP 295
           ++  TG PNQQWV  TQ  P
Sbjct: 302 IYPATGRPNQQWV--TQVLP 315

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_016903609.17.1e-13780.90PREDICTED: nigrin b-like [Cucumis melo][more]
KAA0059853.17.1e-13780.90nigrin b-like [Cucumis melo var. makuwa] >TYK24807.1 nigrin b-like [Cucumis melo... [more]
XP_038885951.15.1e-13579.73nigrin b-like [Benincasa hispida][more]
XP_022150387.18.9e-13279.44nigrin b-like [Momordica charantia] >XP_022156716.1 nigrin b-like [Momordica cha... [more]
KAG5543116.11.2e-7253.54hypothetical protein RHGRI_016012 [Rhododendron griersonianum][more]
Match NameE-valueIdentityDescription
P331831.5e-6848.50Nigrin b OS=Sambucus nigra OX=4202 PE=1 SV=2[more]
Q9M6534.0e-6648.67Ribosome-inactivating protein PMRIPt OS=Polygonatum multiflorum OX=45371 GN=RIPt... [more]
Q413581.5e-6046.92Ribosome-inactivating protein SNAI OS=Sambucus nigra OX=4202 GN=SNA-I PE=1 SV=1[more]
O224151.5e-6046.74Ribosome-inactivating protein SNAIf OS=Sambucus nigra OX=4202 GN=SNA-If PE=1 SV=... [more]
P111409.2e-5544.79Abrin-a OS=Abrus precatorius OX=3816 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A5A7UV903.4e-13780.90Nigrin b-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold184G00550 ... [more]
A0A1S4E5V93.4e-13780.90nigrin b-like OS=Cucumis melo OX=3656 GN=LOC107992255 PE=3 SV=1[more]
A0A6J1DVT24.3e-13279.44nigrin b-like OS=Momordica charantia OX=3673 GN=LOC111023562 PE=3 SV=1[more]
A0A6A4L4D01.9e-7151.16Uncharacterized protein (Fragment) OS=Rhododendron williamsianum OX=262921 GN=C3... [more]
Q8GTA53.4e-6850.38Monomeric lectin SNAlm OS=Sambucus nigra OX=4202 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000772Ricin B, lectin domainSMARTSM00458ricin_3coord: 165..289
e-value: 1.8E-23
score: 94.0
coord: 33..161
e-value: 3.5E-19
score: 79.7
IPR000772Ricin B, lectin domainPFAMPF00652Ricin_B_lectincoord: 36..158
e-value: 1.9E-20
score: 73.4
coord: 172..286
e-value: 1.6E-21
score: 76.9
IPR000772Ricin B, lectin domainPROSITEPS50231RICIN_B_LECTINcoord: 28..161
score: 28.7775
IPR000772Ricin B, lectin domainPROSITEPS50231RICIN_B_LECTINcoord: 164..289
score: 32.092499
IPR000772Ricin B, lectin domainCDDcd00161RICINcoord: 37..160
e-value: 5.22473E-19
score: 79.0866
IPR000772Ricin B, lectin domainCDDcd00161RICINcoord: 171..286
e-value: 7.00151E-22
score: 86.7906
NoneNo IPR availableGENE3D2.80.10.50coord: 31..165
e-value: 2.1E-31
score: 110.7
NoneNo IPR availableGENE3D2.80.10.50coord: 166..290
e-value: 3.5E-42
score: 145.2
NoneNo IPR availablePANTHERPTHR33453:SF23RRNA N-GLYCOSIDASEcoord: 33..289
IPR001574Ribosome-inactivating proteinPANTHERPTHR33453FAMILY NOT NAMEDcoord: 33..289
IPR035992Ricin B-like lectinsSUPERFAMILY50370Ricin B-like lectinscoord: 167..288
IPR035992Ricin B-like lectinsSUPERFAMILY50370Ricin B-like lectinscoord: 34..160

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg014700.1Spg014700.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0017148 negative regulation of translation
molecular_function GO:0030598 rRNA N-glycosylase activity