Spg014599 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg014599 vs. NCBI nr
Match: XP_038896412.1 (rhodanese-like domain-containing protein 4A, chloroplastic [Benincasa hispida])

HSP 1 Score: 574.3 bits (1479), Expect = 8.0e-160
Identity = 318/403 (78.91%), Postives = 336/403 (83.37%), Query Frame = 0

Query: 1   MESQSL---SSPPFLHSHRTIHKLPSQKPISQFFSPIP-FNSKPLSVFNGAHFSSINKTP 60
           MES SL   SS PFLHSHR IHKLPSQKPISQ  S IP  NSKPL+VFNGAHFSSINKTP
Sbjct: 1   MESHSLYLSSSSPFLHSHRKIHKLPSQKPISQSPSLIPSTNSKPLTVFNGAHFSSINKTP 60

Query: 61  LPNPTNPSKPLQNPQFLFSKTSSFHELAKTHLPFALTQ-LLLTPLPGFALEADQA-SDKI 120
            P PT PS    NP FLF+KTS FH+LAK++LPFALTQ LLLTPLP FALE DQA SDKI
Sbjct: 61  FPTPTIPS----NPHFLFAKTSPFHQLAKSYLPFALTQLLLLTPLPSFALETDQASSDKI 120

Query: 121 NLESILLSIDDFFNRYPFFVAGCTFIWLVGIPLIEYFF-RKYKFVSAIDAFRKIRDDPNA 180
           NLESIL+SIDDFFNRYPFFVAGCTFIWLVGIPLI+YFF RKYKF+SAIDAFRKIRD+PNA
Sbjct: 121 NLESILVSIDDFFNRYPFFVAGCTFIWLVGIPLIDYFFLRKYKFISAIDAFRKIRDEPNA 180

Query: 181 QLLDIRDEKSLAFLGSPNLRILNKDVVQVVYSEEDEDGFVKKVKKSLGNGADTVVFVLDN 240
           QLLDIRDEKSLAFLGSPNLRILNKDVVQVVYSEEDEDGFVKKVK S G+ ADT+VF+LDN
Sbjct: 181 QLLDIRDEKSLAFLGSPNLRILNKDVVQVVYSEEDEDGFVKKVKNSFGDVADTLVFILDN 240

Query: 241 FDGNSMKVAELLFKNGFKEAYAIKDGVRGEKGWLDLVLLYGFSSTTAQGHKLPPNAIQES 300
           FDGNSMKVAELL KNGFKEAYAIKDGVRGEKGWL                     AIQES
Sbjct: 241 FDGNSMKVAELLVKNGFKEAYAIKDGVRGEKGWL---------------------AIQES 300

Query: 301 LLPPSVHMNRRRKAKASQKFGTNGVVQQNGDNKAAPSSKSSNGEIQTSRLHVDASSDTKS 360
           LLPPSVHMNRR+KAKASQK GTNGVVQQNGDNKA PS KSSNGEIQTSRLHV+ASS T++
Sbjct: 301 LLPPSVHMNRRKKAKASQKLGTNGVVQQNGDNKAVPSLKSSNGEIQTSRLHVNASSATET 360

Query: 361 QLKTQSRSSSPYPNTDSVFIYTDIWQYPDLKPPSSPTPSKPGQ 397
           QLKT+SR SSPYPN            YPDLKPPSSPTPSKPG+
Sbjct: 361 QLKTRSRPSSPYPN------------YPDLKPPSSPTPSKPGK 366

BLAST of Spg014599 vs. NCBI nr
Match: KAG7014541.1 (Rhodanese-like domain-containing protein 4A, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 564.7 bits (1454), Expect = 6.3e-157
Identity = 307/399 (76.94%), Postives = 331/399 (82.96%), Query Frame = 0

Query: 1   MESQSL---SSPPFLHSHRTIHKLPSQKPISQFFSPIPFNSKPLSVFNGAHFSSINKTPL 60
           MES SL   SS  FL      HKLPS KPIS   S IPFNSKPLSVFNGAH  +INKTP 
Sbjct: 1   MESHSLYLSSSSLFL------HKLPSHKPISLSPSLIPFNSKPLSVFNGAHLPTINKTPF 60

Query: 61  PNPTNPSKPLQNPQFLFSKTSSFHELAKTHLPFALTQLLLTPLPGFALEADQA-SDKINL 120
            NPTNPSKPL+NP FLF+KTS FH+L K+HLPFALTQLLL+PLP  ALE DQA SDKINL
Sbjct: 61  QNPTNPSKPLRNPHFLFAKTSPFHQLVKSHLPFALTQLLLSPLPSLALETDQASSDKINL 120

Query: 121 ESILLSIDDFFNRYPFFVAGCTFIWLVGIPLIEYFF-RKYKFVSAIDAFRKIRDDPNAQL 180
           ESIL+SIDDFFNRYPFFVAGCTFIWLVGIPLI+Y+F RKYKF+SAIDAFR+IRDDPNAQL
Sbjct: 121 ESILVSIDDFFNRYPFFVAGCTFIWLVGIPLIDYYFLRKYKFISAIDAFRRIRDDPNAQL 180

Query: 181 LDIRDEKSLAFLGSPNLRILNKDVVQVVYSEEDEDGFVKKVKKSLGNGADTVVFVLDNFD 240
           LDIRDEKSLAFLGSPNLRILNKDVVQVVYSEEDEDGFVKKVKK  G+GADT+VFVLDNFD
Sbjct: 181 LDIRDEKSLAFLGSPNLRILNKDVVQVVYSEEDEDGFVKKVKKGFGDGADTLVFVLDNFD 240

Query: 241 GNSMKVAELLFKNGFKEAYAIKDGVRGEKGWLDLVLLYGFSSTTAQGHKLPPNAIQESLL 300
           GNSMKVAELLFKNGFKEAYAIKDGVRGEKGW+                     AIQESLL
Sbjct: 241 GNSMKVAELLFKNGFKEAYAIKDGVRGEKGWM---------------------AIQESLL 300

Query: 301 PPSVHMNRRRKAKASQKFGTNGVVQQNGDNKAAPSSKSSNGEIQTSRLHVDASSDTKSQL 360
           PPSVH++RR+KAKAS+KFGTNGVVQQ+GD +A PS KSSNGEIQTS LHV+ASSDT+S+L
Sbjct: 301 PPSVHISRRKKAKASKKFGTNGVVQQSGDKEAVPSLKSSNGEIQTSPLHVNASSDTESKL 360

Query: 361 KTQSRSSSPYPNTDSVFIYTDIWQYPDLKPPSSPTPSKP 395
           KT+SRSSSPYPN            YPDLKPPSSPTPSKP
Sbjct: 361 KTRSRSSSPYPN------------YPDLKPPSSPTPSKP 360

BLAST of Spg014599 vs. NCBI nr
Match: XP_022991517.1 (rhodanese-like domain-containing protein 4A, chloroplastic [Cucurbita maxima])

HSP 1 Score: 561.6 bits (1446), Expect = 5.4e-156
Identity = 308/399 (77.19%), Postives = 328/399 (82.21%), Query Frame = 0

Query: 1   MESQSL---SSPPFLHSHRTIHKLPSQKPISQFFSPIPFNSKPLSVFNGAHFSSINKTPL 60
           MES SL   SS  FL      HKLPS KPIS   S IPFNSKPLSVFNG HF SINKTP 
Sbjct: 1   MESHSLYLSSSSLFL------HKLPSHKPISLSPSLIPFNSKPLSVFNGTHFPSINKTPF 60

Query: 61  PNPTNPSKPLQNPQFLFSKTSSFHELAKTHLPFALTQLLLTPLPGFALEADQA-SDKINL 120
            NPTNPS PL+NP FLF+KTS F +L K+HLPFALTQLLLTPLP  ALE DQA SDKINL
Sbjct: 61  QNPTNPSNPLRNPHFLFAKTSPFLQLVKSHLPFALTQLLLTPLPSLALETDQASSDKINL 120

Query: 121 ESILLSIDDFFNRYPFFVAGCTFIWLVGIPLIEYFF-RKYKFVSAIDAFRKIRDDPNAQL 180
           ESIL+SIDDFFNRYPFFVAGCTFIWLVGIPLI+Y+F RKYKF+SAIDAFR+IRDDPNAQL
Sbjct: 121 ESILVSIDDFFNRYPFFVAGCTFIWLVGIPLIDYYFLRKYKFISAIDAFRRIRDDPNAQL 180

Query: 181 LDIRDEKSLAFLGSPNLRILNKDVVQVVYSEEDEDGFVKKVKKSLGNGADTVVFVLDNFD 240
           LDIRDEKSLAFLGSPNLRILNKDVVQVVYSEEDEDGFVKKVKK  G+GADT VFVLDNFD
Sbjct: 181 LDIRDEKSLAFLGSPNLRILNKDVVQVVYSEEDEDGFVKKVKKGFGDGADTQVFVLDNFD 240

Query: 241 GNSMKVAELLFKNGFKEAYAIKDGVRGEKGWLDLVLLYGFSSTTAQGHKLPPNAIQESLL 300
           GNSMKVAELLFKNGFKEAYAIKDGVRGEKGWL                     AIQESLL
Sbjct: 241 GNSMKVAELLFKNGFKEAYAIKDGVRGEKGWL---------------------AIQESLL 300

Query: 301 PPSVHMNRRRKAKASQKFGTNGVVQQNGDNKAAPSSKSSNGEIQTSRLHVDASSDTKSQL 360
           PPSVH++RR+KAKAS+KFGTNGVVQQ+GD +A PS KSSNGEIQTS LHV+ASSDT+S+L
Sbjct: 301 PPSVHISRRKKAKASKKFGTNGVVQQSGDKEAVPSLKSSNGEIQTSPLHVNASSDTESKL 360

Query: 361 KTQSRSSSPYPNTDSVFIYTDIWQYPDLKPPSSPTPSKP 395
           KT+SRSSSPYPN            YPDLKPPSSPTPSKP
Sbjct: 361 KTRSRSSSPYPN------------YPDLKPPSSPTPSKP 360

BLAST of Spg014599 vs. NCBI nr
Match: KAG6576020.1 (Rhodanese-like domain-containing protein 4A, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 558.9 bits (1439), Expect = 3.5e-155
Identity = 306/402 (76.12%), Postives = 331/402 (82.34%), Query Frame = 0

Query: 1   MESQSL---SSPPFLHSHRTIHKLPSQKPISQFFSPIPFNSKPLSVFNGAHFSSINKTPL 60
           MES SL   SS  FL      HKLPS KPIS   S IPFNSKPLSVFNGAH  +INKTP 
Sbjct: 1   MESHSLYLSSSSLFL------HKLPSHKPISLSPSLIPFNSKPLSVFNGAHLPTINKTPF 60

Query: 61  PNPTNPSKPLQNPQFLFSKTSSFHELAKTHLPFALTQLLLTPLPGFALEADQA-SDKINL 120
            NPTNPSKPL+NP FLF+KTS FH+L K+HLPFALTQLLL+PLP  ALE DQA SDKINL
Sbjct: 61  QNPTNPSKPLRNPHFLFAKTSPFHQLVKSHLPFALTQLLLSPLPSLALETDQASSDKINL 120

Query: 121 ESILLSIDDFFNRYPFFVAGCTFIWLVGIPLIEYFF-RKYKFVSAIDAFRKIRDDPNAQL 180
           ESIL+SIDDFFNRYPFFVAGCTFIWLVGIPLI+Y+F RKYKF+SAIDAFR+IRDDPNAQL
Sbjct: 121 ESILVSIDDFFNRYPFFVAGCTFIWLVGIPLIDYYFLRKYKFISAIDAFRRIRDDPNAQL 180

Query: 181 LDIRDEKSLAFLGSPNLRILNKDVVQVVYSEEDEDGF---VKKVKKSLGNGADTVVFVLD 240
           LDIRDEKSLAFLGSPNLRILNKDVVQVVYSEEDEDGF   VKKVKK  G+GADT+VFVLD
Sbjct: 181 LDIRDEKSLAFLGSPNLRILNKDVVQVVYSEEDEDGFVKKVKKVKKGFGDGADTLVFVLD 240

Query: 241 NFDGNSMKVAELLFKNGFKEAYAIKDGVRGEKGWLDLVLLYGFSSTTAQGHKLPPNAIQE 300
           NFDGNSMKVAELLFKNGFKEAYAIKDGVRGEKGW+                     AIQE
Sbjct: 241 NFDGNSMKVAELLFKNGFKEAYAIKDGVRGEKGWM---------------------AIQE 300

Query: 301 SLLPPSVHMNRRRKAKASQKFGTNGVVQQNGDNKAAPSSKSSNGEIQTSRLHVDASSDTK 360
           SLLPPSVH++RR+KAKAS+KFGTNGVVQQ+GD +A PS KSSNGE+QTS LHV+ASSDT+
Sbjct: 301 SLLPPSVHISRRKKAKASKKFGTNGVVQQSGDKEAVPSLKSSNGEVQTSPLHVNASSDTE 360

Query: 361 SQLKTQSRSSSPYPNTDSVFIYTDIWQYPDLKPPSSPTPSKP 395
           S+LKT+SRSSSPYPN            YPDLKPPSSPTPSKP
Sbjct: 361 SKLKTRSRSSSPYPN------------YPDLKPPSSPTPSKP 363

BLAST of Spg014599 vs. NCBI nr
Match: XP_022954032.1 (rhodanese-like domain-containing protein 4A, chloroplastic [Cucurbita moschata])

HSP 1 Score: 557.8 bits (1436), Expect = 7.7e-155
Identity = 304/399 (76.19%), Postives = 329/399 (82.46%), Query Frame = 0

Query: 1   MESQSL---SSPPFLHSHRTIHKLPSQKPISQFFSPIPFNSKPLSVFNGAHFSSINKTPL 60
           MES SL   SS  FL      HKLPS KPIS   S IPFNSKPLSVFNGAH  +INKTP 
Sbjct: 1   MESHSLYLSSSSLFL------HKLPSHKPISLSPSLIPFNSKPLSVFNGAHLPTINKTPF 60

Query: 61  PNPTNPSKPLQNPQFLFSKTSSFHELAKTHLPFALTQLLLTPLPGFALEADQA-SDKINL 120
            NPTNPSKPL+NP FLF+KTS FH+L K+HLPFALTQLLL+PLP  ALE DQA SDKINL
Sbjct: 61  QNPTNPSKPLRNPHFLFAKTSPFHQLVKSHLPFALTQLLLSPLPSLALETDQASSDKINL 120

Query: 121 ESILLSIDDFFNRYPFFVAGCTFIWLVGIPLIEYFF-RKYKFVSAIDAFRKIRDDPNAQL 180
           ESIL+SIDDFFNRYPFFVAGCTFIWLVGIPLI+Y+F RKYKF+SAIDAFR+IRDDPNAQL
Sbjct: 121 ESILVSIDDFFNRYPFFVAGCTFIWLVGIPLIDYYFLRKYKFISAIDAFRRIRDDPNAQL 180

Query: 181 LDIRDEKSLAFLGSPNLRILNKDVVQVVYSEEDEDGFVKKVKKSLGNGADTVVFVLDNFD 240
           LDIRDEKSLAFLGSPNLRILNKDVVQVVYSEEDEDGFVKKVKK  G+GADT+VFVLDNFD
Sbjct: 181 LDIRDEKSLAFLGSPNLRILNKDVVQVVYSEEDEDGFVKKVKKGFGDGADTLVFVLDNFD 240

Query: 241 GNSMKVAELLFKNGFKEAYAIKDGVRGEKGWLDLVLLYGFSSTTAQGHKLPPNAIQESLL 300
           GNSMKVAELLFKNGFKEAYAIKDGVRGEKGW+                     AIQESLL
Sbjct: 241 GNSMKVAELLFKNGFKEAYAIKDGVRGEKGWM---------------------AIQESLL 300

Query: 301 PPSVHMNRRRKAKASQKFGTNGVVQQNGDNKAAPSSKSSNGEIQTSRLHVDASSDTKSQL 360
           PPSVH++RR+KAKAS+KFGTNGVVQ++ D +A PS  SSNGEIQTS LHV+ASSDT+S+L
Sbjct: 301 PPSVHISRRKKAKASKKFGTNGVVQKSVDKEAVPSLNSSNGEIQTSPLHVNASSDTESKL 360

Query: 361 KTQSRSSSPYPNTDSVFIYTDIWQYPDLKPPSSPTPSKP 395
           KT+SRSSSPYPN            YPDLKPPSSPTPSKP
Sbjct: 361 KTRSRSSSPYPN------------YPDLKPPSSPTPSKP 360

BLAST of Spg014599 vs. ExPASy Swiss-Prot
Match: Q56XR7 (Rhodanese-like domain-containing protein 4A, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=STR4A PE=2 SV=1)

HSP 1 Score: 233.0 bits (593), Expect = 5.7e-60
Identity = 136/280 (48.57%), Postives = 177/280 (63.21%), Query Frame = 0

Query: 55  TPLPNPTNP------SKPLQNPQFLFSKTSSFHELAKTHLPFALTQLLLTPLPGFALE-- 114
           +PL N T P       KP+QN     +K    H L KT+L   ++QL++T  P  A E  
Sbjct: 11  SPLRNLTKPCSTSQIPKPIQNS----TKQPPIHLLTKTNLSVTISQLIITS-PVLASESF 70

Query: 115 ----ADQASDKINLESILLSIDDFFNRYPFFVAGCTFIWLVGIPLIEYFFRKYKFVSAID 174
               +D +S KI+LESIL++ID+FFN+YPFFVAGCTF +LV  P + ++ RKYK +SA++
Sbjct: 71  DSISSDPSSGKIDLESILVTIDNFFNKYPFFVAGCTFTYLVVYPAVMFYLRKYKPISAMN 130

Query: 175 AFRKIRDDPNAQLLDIRDEKSLAFLGSPNLRILNKDVVQVVYSEEDEDGFVKKVKKSLGN 234
           AFRK++++ ++QLLDIRD K+LA L SPNL+ L K  VQV +SE DE+GF+ KVK S  +
Sbjct: 131 AFRKLKNESDSQLLDIRDVKTLALLASPNLKFLGKSSVQVPFSENDEEGFLTKVKGSFSD 190

Query: 235 GADTVVFVLDNFDGNSMKVAELLFKNGFKEAYAIKDGVRGEKGWLDLVLLYGFSSTTAQG 294
             +TVV VLDNFDGNS KVAELL KNGFKEAY I+ G RG+ GWL               
Sbjct: 191 AENTVVCVLDNFDGNSSKVAELLIKNGFKEAYYIRGGARGKNGWL--------------- 250

Query: 295 HKLPPNAIQESLLPPSVHMNRRRKAKASQKFGTNGVVQQN 323
                 AIQE LLPP VHM   +  K+S     + V  +N
Sbjct: 251 ------AIQEELLPPPVHMYTAKNVKSSNNNEASVVGTEN 264

BLAST of Spg014599 vs. ExPASy Swiss-Prot
Match: Q9M158 (Rhodanese-like domain-containing protein 4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=STR4 PE=1 SV=2)

HSP 1 Score: 115.5 bits (288), Expect = 1.3e-24
Identity = 78/234 (33.33%), Postives = 113/234 (48.29%), Query Frame = 0

Query: 40  PLSVFNGAHFSSINKTPLPN--PTNPSKPLQNPQFLFSKTSSFHELAKTHLPFALTQLLL 99
           P+SV +           LPN  P    +P+    FL  + +    L  + L  A      
Sbjct: 11  PMSVLSEKRSEPRKPFSLPNLFPPKSQRPISQESFL-KRFNGGLALLTSVLSSATAPAKS 70

Query: 100 TPLPGFALEADQASDKINLESILLSIDDFFNRYPFFVAGCTFIWLVGIPLIEYFFRKYK- 159
                   ++   S   + + ++  I +F    P  +AG      V   L +   +K K 
Sbjct: 71  LTYEEALQQSMTTSSSFDSDGLIEGISNFVTDNPLVIAGGVAALAVPFVLSQVLNKKPKS 130

Query: 160 --FVSAIDAFRKIRDDPNAQLLDIRDEKSLAFLGSPNLRILNKDVVQVVYSEEDEDGFVK 219
               SA +A+ K+  D NAQLLDIR       +GSPN++ L K  V  VY+ ED+ GF+K
Sbjct: 131 WGVESAKNAYTKLGTDDNAQLLDIRATADFRQVGSPNIKGLGKKAVSTVYNGEDKPGFLK 190

Query: 220 KVKKSLGNGADTVVFVLDNFDGNSMKVAELLFKNGFKEAYAIKDGVRGEKGWLD 269
           K+     +  +T +++LD FDGNS  VAEL+  NGFK AYAIKDG  G +GWL+
Sbjct: 191 KLSLKFKDPENTTLYILDKFDGNSELVAELVALNGFKSAYAIKDGAEGPRGWLN 243

BLAST of Spg014599 vs. ExPASy Swiss-Prot
Match: A2X345 (Protein THYLAKOID RHODANESE-LIKE, chloroplastic OS=Oryza sativa subsp. indica OX=39946 GN=TROL PE=3 SV=1)

HSP 1 Score: 102.8 bits (255), Expect = 8.9e-21
Identity = 50/115 (43.48%), Postives = 71/115 (61.74%), Query Frame = 0

Query: 153 RKYKFVSAIDAFRKIRDDPNAQLLDIRDEKSLAFLGSPNLRILNKDVVQVVYSEEDEDGF 212
           + Y  VSA  A+R + ++P AQL+DIR        G+P+LR   K    V Y  ED++GF
Sbjct: 124 KPYGVVSAAAAYRALVEEPGAQLVDIRPPGDARQSGAPDLREAKKKAAAVPYDGEDKNGF 183

Query: 213 VKKVKKSLGNGADTVVFVLDNFDGNSMKVAELLFKNGFKEAYAIKDGVRGEKGWL 268
           +KK+     +  +T + +LD FDGNS  VAEL+  NG+K A+A+KDG  G +GWL
Sbjct: 184 LKKLSLRFKDPENTTLVILDKFDGNSELVAELVTANGYKAAFAVKDGAEGRRGWL 238

BLAST of Spg014599 vs. ExPASy Swiss-Prot
Match: Q6ETQ7 (Protein THYLAKOID RHODANESE-LIKE, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=TROL PE=1 SV=1)

HSP 1 Score: 102.8 bits (255), Expect = 8.9e-21
Identity = 50/115 (43.48%), Postives = 71/115 (61.74%), Query Frame = 0

Query: 153 RKYKFVSAIDAFRKIRDDPNAQLLDIRDEKSLAFLGSPNLRILNKDVVQVVYSEEDEDGF 212
           + Y  VSA  A+R + ++P AQL+DIR        G+P+LR   K    V Y  ED++GF
Sbjct: 126 KPYGVVSAAAAYRALVEEPGAQLVDIRPPGDARQSGAPDLREAKKKAAAVPYDGEDKNGF 185

Query: 213 VKKVKKSLGNGADTVVFVLDNFDGNSMKVAELLFKNGFKEAYAIKDGVRGEKGWL 268
           +KK+     +  +T + +LD FDGNS  VAEL+  NG+K A+A+KDG  G +GWL
Sbjct: 186 LKKLSLRFKDPENTTLVILDKFDGNSELVAELVTANGYKAAFAVKDGAEGRRGWL 240

BLAST of Spg014599 vs. ExPASy TrEMBL
Match: A0A6J1JM16 (rhodanese-like domain-containing protein 4A, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111488109 PE=4 SV=1)

HSP 1 Score: 561.6 bits (1446), Expect = 2.6e-156
Identity = 308/399 (77.19%), Postives = 328/399 (82.21%), Query Frame = 0

Query: 1   MESQSL---SSPPFLHSHRTIHKLPSQKPISQFFSPIPFNSKPLSVFNGAHFSSINKTPL 60
           MES SL   SS  FL      HKLPS KPIS   S IPFNSKPLSVFNG HF SINKTP 
Sbjct: 1   MESHSLYLSSSSLFL------HKLPSHKPISLSPSLIPFNSKPLSVFNGTHFPSINKTPF 60

Query: 61  PNPTNPSKPLQNPQFLFSKTSSFHELAKTHLPFALTQLLLTPLPGFALEADQA-SDKINL 120
            NPTNPS PL+NP FLF+KTS F +L K+HLPFALTQLLLTPLP  ALE DQA SDKINL
Sbjct: 61  QNPTNPSNPLRNPHFLFAKTSPFLQLVKSHLPFALTQLLLTPLPSLALETDQASSDKINL 120

Query: 121 ESILLSIDDFFNRYPFFVAGCTFIWLVGIPLIEYFF-RKYKFVSAIDAFRKIRDDPNAQL 180
           ESIL+SIDDFFNRYPFFVAGCTFIWLVGIPLI+Y+F RKYKF+SAIDAFR+IRDDPNAQL
Sbjct: 121 ESILVSIDDFFNRYPFFVAGCTFIWLVGIPLIDYYFLRKYKFISAIDAFRRIRDDPNAQL 180

Query: 181 LDIRDEKSLAFLGSPNLRILNKDVVQVVYSEEDEDGFVKKVKKSLGNGADTVVFVLDNFD 240
           LDIRDEKSLAFLGSPNLRILNKDVVQVVYSEEDEDGFVKKVKK  G+GADT VFVLDNFD
Sbjct: 181 LDIRDEKSLAFLGSPNLRILNKDVVQVVYSEEDEDGFVKKVKKGFGDGADTQVFVLDNFD 240

Query: 241 GNSMKVAELLFKNGFKEAYAIKDGVRGEKGWLDLVLLYGFSSTTAQGHKLPPNAIQESLL 300
           GNSMKVAELLFKNGFKEAYAIKDGVRGEKGWL                     AIQESLL
Sbjct: 241 GNSMKVAELLFKNGFKEAYAIKDGVRGEKGWL---------------------AIQESLL 300

Query: 301 PPSVHMNRRRKAKASQKFGTNGVVQQNGDNKAAPSSKSSNGEIQTSRLHVDASSDTKSQL 360
           PPSVH++RR+KAKAS+KFGTNGVVQQ+GD +A PS KSSNGEIQTS LHV+ASSDT+S+L
Sbjct: 301 PPSVHISRRKKAKASKKFGTNGVVQQSGDKEAVPSLKSSNGEIQTSPLHVNASSDTESKL 360

Query: 361 KTQSRSSSPYPNTDSVFIYTDIWQYPDLKPPSSPTPSKP 395
           KT+SRSSSPYPN            YPDLKPPSSPTPSKP
Sbjct: 361 KTRSRSSSPYPN------------YPDLKPPSSPTPSKP 360

BLAST of Spg014599 vs. ExPASy TrEMBL
Match: A0A6J1GPW4 (rhodanese-like domain-containing protein 4A, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111456414 PE=4 SV=1)

HSP 1 Score: 557.8 bits (1436), Expect = 3.7e-155
Identity = 304/399 (76.19%), Postives = 329/399 (82.46%), Query Frame = 0

Query: 1   MESQSL---SSPPFLHSHRTIHKLPSQKPISQFFSPIPFNSKPLSVFNGAHFSSINKTPL 60
           MES SL   SS  FL      HKLPS KPIS   S IPFNSKPLSVFNGAH  +INKTP 
Sbjct: 1   MESHSLYLSSSSLFL------HKLPSHKPISLSPSLIPFNSKPLSVFNGAHLPTINKTPF 60

Query: 61  PNPTNPSKPLQNPQFLFSKTSSFHELAKTHLPFALTQLLLTPLPGFALEADQA-SDKINL 120
            NPTNPSKPL+NP FLF+KTS FH+L K+HLPFALTQLLL+PLP  ALE DQA SDKINL
Sbjct: 61  QNPTNPSKPLRNPHFLFAKTSPFHQLVKSHLPFALTQLLLSPLPSLALETDQASSDKINL 120

Query: 121 ESILLSIDDFFNRYPFFVAGCTFIWLVGIPLIEYFF-RKYKFVSAIDAFRKIRDDPNAQL 180
           ESIL+SIDDFFNRYPFFVAGCTFIWLVGIPLI+Y+F RKYKF+SAIDAFR+IRDDPNAQL
Sbjct: 121 ESILVSIDDFFNRYPFFVAGCTFIWLVGIPLIDYYFLRKYKFISAIDAFRRIRDDPNAQL 180

Query: 181 LDIRDEKSLAFLGSPNLRILNKDVVQVVYSEEDEDGFVKKVKKSLGNGADTVVFVLDNFD 240
           LDIRDEKSLAFLGSPNLRILNKDVVQVVYSEEDEDGFVKKVKK  G+GADT+VFVLDNFD
Sbjct: 181 LDIRDEKSLAFLGSPNLRILNKDVVQVVYSEEDEDGFVKKVKKGFGDGADTLVFVLDNFD 240

Query: 241 GNSMKVAELLFKNGFKEAYAIKDGVRGEKGWLDLVLLYGFSSTTAQGHKLPPNAIQESLL 300
           GNSMKVAELLFKNGFKEAYAIKDGVRGEKGW+                     AIQESLL
Sbjct: 241 GNSMKVAELLFKNGFKEAYAIKDGVRGEKGWM---------------------AIQESLL 300

Query: 301 PPSVHMNRRRKAKASQKFGTNGVVQQNGDNKAAPSSKSSNGEIQTSRLHVDASSDTKSQL 360
           PPSVH++RR+KAKAS+KFGTNGVVQ++ D +A PS  SSNGEIQTS LHV+ASSDT+S+L
Sbjct: 301 PPSVHISRRKKAKASKKFGTNGVVQKSVDKEAVPSLNSSNGEIQTSPLHVNASSDTESKL 360

Query: 361 KTQSRSSSPYPNTDSVFIYTDIWQYPDLKPPSSPTPSKP 395
           KT+SRSSSPYPN            YPDLKPPSSPTPSKP
Sbjct: 361 KTRSRSSSPYPN------------YPDLKPPSSPTPSKP 360

BLAST of Spg014599 vs. ExPASy TrEMBL
Match: A0A5D3B9W6 (Rhodanese-like domain-containing protein 4A OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold549G00060 PE=4 SV=1)

HSP 1 Score: 527.3 bits (1357), Expect = 5.4e-146
Identity = 296/402 (73.63%), Postives = 321/402 (79.85%), Query Frame = 0

Query: 1   MESQSL---SSPPFLHSHRTIHKLPSQKPISQFFSPIPF-NSKPLSVFNGAHFSSINKTP 60
           MES SL   SS PFL SHRTIHKL S+KPIS+  S IPF NSKPL+VFNG HFSSIN+T 
Sbjct: 1   MESHSLYLSSSSPFLSSHRTIHKLSSEKPISRPPSLIPFANSKPLTVFNGPHFSSINRTS 60

Query: 61  LPNPTNPSKPLQNPQFLFSKTSSFHELAKTHLPFALTQ-LLLTPLPGFALEADQA-SDKI 120
            P+PT PS    NP+FLF+KTS   +L K H PFALTQ LLLTPLP FALE D A SDKI
Sbjct: 61  FPSPTIPS----NPRFLFAKTSPILQLTKPHFPFALTQLLLLTPLPSFALETDLASSDKI 120

Query: 121 NLESILLSIDDFFNRYPFFVAGCTFIWLVGIPLIEYFF-RKYKFVSAIDAFRKIRDDPNA 180
           NLESIL+SID+FFNRYPFFVAGCTFIWLV IPLI+Y+F RKYKF+SAIDAFRKIRD+PNA
Sbjct: 121 NLESILVSIDEFFNRYPFFVAGCTFIWLVVIPLIDYYFIRKYKFISAIDAFRKIRDEPNA 180

Query: 181 QLLDIRDEKSLAFLGSPNLRILNKDVVQVVYSEEDEDGFVKKVKKSLGNGADTVVFVLDN 240
           QLLD+R+EKSLA LGSPNLRILNKDVV+VVYSEEDEDGFVKKVKKS G+ ADT+VFVLDN
Sbjct: 181 QLLDVREEKSLAVLGSPNLRILNKDVVRVVYSEEDEDGFVKKVKKSFGDCADTIVFVLDN 240

Query: 241 FDGNSMKVAELLFKNGFKEAYAIKDGVRGEKGWLDLVLLYGFSSTTAQGHKLPPNAIQES 300
           FDGNSMKVAELL KNGFKEAYAIKDGVRGEKGWL                     AIQES
Sbjct: 241 FDGNSMKVAELLVKNGFKEAYAIKDGVRGEKGWL---------------------AIQES 300

Query: 301 LLPPSVHMNRRRKAKASQKFGTNGVVQQNGDNKAAPSSKSSNGEIQTSRLHVDASSDTKS 360
           LLPPSVHM RR+K KASQK GTNGVVQQNGDN+  PS KSSN EIQ S++HV+ASS  +S
Sbjct: 301 LLPPSVHMKRRKKGKASQKLGTNGVVQQNGDNETVPSLKSSNREIQPSQVHVNASSPAES 360

Query: 361 QLKTQSRSSSPYPNTDSVFIYTDIWQYPDLKPPSSPTPSKPG 396
           QLKT SR SSPYPN            YPDLKPPSSPTPSKPG
Sbjct: 361 QLKTWSRPSSPYPN------------YPDLKPPSSPTPSKPG 365

BLAST of Spg014599 vs. ExPASy TrEMBL
Match: A0A1S3C7N8 (rhodanese-like domain-containing protein 4A, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103497630 PE=4 SV=1)

HSP 1 Score: 527.3 bits (1357), Expect = 5.4e-146
Identity = 296/402 (73.63%), Postives = 321/402 (79.85%), Query Frame = 0

Query: 1   MESQSL---SSPPFLHSHRTIHKLPSQKPISQFFSPIPF-NSKPLSVFNGAHFSSINKTP 60
           MES SL   SS PFL SHRTIHKL S+KPIS+  S IPF NSKPL+VFNG HFSSIN+T 
Sbjct: 1   MESHSLYLSSSSPFLSSHRTIHKLSSEKPISRPPSLIPFANSKPLTVFNGPHFSSINRTS 60

Query: 61  LPNPTNPSKPLQNPQFLFSKTSSFHELAKTHLPFALTQ-LLLTPLPGFALEADQA-SDKI 120
            P+PT PS    NP+FLF+KTS   +L K H PFALTQ LLLTPLP FALE D A SDKI
Sbjct: 61  FPSPTIPS----NPRFLFAKTSPILQLTKPHFPFALTQLLLLTPLPSFALETDLASSDKI 120

Query: 121 NLESILLSIDDFFNRYPFFVAGCTFIWLVGIPLIEYFF-RKYKFVSAIDAFRKIRDDPNA 180
           NLESIL+SID+FFNRYPFFVAGCTFIWLV IPLI+Y+F RKYKF+SAIDAFRKIRD+PNA
Sbjct: 121 NLESILVSIDEFFNRYPFFVAGCTFIWLVVIPLIDYYFIRKYKFISAIDAFRKIRDEPNA 180

Query: 181 QLLDIRDEKSLAFLGSPNLRILNKDVVQVVYSEEDEDGFVKKVKKSLGNGADTVVFVLDN 240
           QLLD+R+EKSLA LGSPNLRILNKDVV+VVYSEEDEDGFVKKVKKS G+ ADT+VFVLDN
Sbjct: 181 QLLDVREEKSLAVLGSPNLRILNKDVVRVVYSEEDEDGFVKKVKKSFGDCADTIVFVLDN 240

Query: 241 FDGNSMKVAELLFKNGFKEAYAIKDGVRGEKGWLDLVLLYGFSSTTAQGHKLPPNAIQES 300
           FDGNSMKVAELL KNGFKEAYAIKDGVRGEKGWL                     AIQES
Sbjct: 241 FDGNSMKVAELLVKNGFKEAYAIKDGVRGEKGWL---------------------AIQES 300

Query: 301 LLPPSVHMNRRRKAKASQKFGTNGVVQQNGDNKAAPSSKSSNGEIQTSRLHVDASSDTKS 360
           LLPPSVHM RR+K KASQK GTNGVVQQNGDN+  PS KSSN EIQ S++HV+ASS  +S
Sbjct: 301 LLPPSVHMKRRKKGKASQKLGTNGVVQQNGDNETVPSLKSSNREIQPSQVHVNASSPAES 360

Query: 361 QLKTQSRSSSPYPNTDSVFIYTDIWQYPDLKPPSSPTPSKPG 396
           QLKT SR SSPYPN            YPDLKPPSSPTPSKPG
Sbjct: 361 QLKTWSRPSSPYPN------------YPDLKPPSSPTPSKPG 365

BLAST of Spg014599 vs. ExPASy TrEMBL
Match: A0A0A0K8H6 (Rhodanese domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G368730 PE=4 SV=1)

HSP 1 Score: 516.9 bits (1330), Expect = 7.3e-143
Identity = 292/403 (72.46%), Postives = 316/403 (78.41%), Query Frame = 0

Query: 1   MESQSL---SSPPFLHSHRTIHKLPSQKPISQFFSPIPF-NSKPLSVFNGAHFSSINKTP 60
           MES SL   SS P L SHR IHKL SQKPIS   S IPF NSKPL+VFNG  FSSIN+TP
Sbjct: 1   MESHSLYLSSSSPSLQSHRAIHKLSSQKPISPPPSLIPFANSKPLTVFNGPRFSSINRTP 60

Query: 61  LPNPTNPSKPLQNPQFLFSKTSSFHELAKTHLPFALTQ-LLLTPLPGFALEADQA-SDKI 120
            P+PT PS    NP FLF+KTS F +L K H PFALTQ LLLTPLP FALE D A SDKI
Sbjct: 61  FPSPTIPS----NPPFLFAKTSPFLQLMKPHFPFALTQLLLLTPLPSFALETDLASSDKI 120

Query: 121 NLESILLSIDDFFNRYPFFVAGCTFIWLVGIPLIEYFF-RKYKFVSAIDAFRKIRDDPNA 180
           +LESIL+SID+FFNRYPFFVAGCTFIWLV IP I+Y+F RKYKF+SAI+AFRKIRD+PNA
Sbjct: 121 SLESILVSIDEFFNRYPFFVAGCTFIWLVVIPSIDYYFIRKYKFISAINAFRKIRDEPNA 180

Query: 181 QLLDIRDEKSLAFLGSPNLRILNKDVVQVVYSEEDEDGFVKKVKKSLGNGADTVVFVLDN 240
           QLLD+R+EKSLA LGSPNLRILNKDVV+VVYSEEDEDGFVKKVKKS G+ ADT+VFVLDN
Sbjct: 181 QLLDVREEKSLAVLGSPNLRILNKDVVRVVYSEEDEDGFVKKVKKSFGDDADTIVFVLDN 240

Query: 241 FDGNSMKVAELLFKNGFKEAYAIKDGVRGEKGWLDLVLLYGFSSTTAQGHKLPPNAIQES 300
           FDGNSMK AELL KNGFKEAYAIKDGVRGEKGWL                     AIQES
Sbjct: 241 FDGNSMKAAELLVKNGFKEAYAIKDGVRGEKGWL---------------------AIQES 300

Query: 301 LLPPSVHMNRRRKAKASQKFGTNGVVQQNGDNKAAPSSKSSNGEIQTSRLHVDASSDTKS 360
           LLPPSVHM RR+K KAS K GTNGVVQQNGDN+A PS KSSN EIQ S +HV+ASS  +S
Sbjct: 301 LLPPSVHMKRRKKGKASPKLGTNGVVQQNGDNEAVPSLKSSNREIQPSHVHVNASSPAES 360

Query: 361 QLKTQSRSSSPYPNTDSVFIYTDIWQYPDLKPPSSPTPSKPGQ 397
           QLKTQSR SSPYPN            YPDLKPPSSP PSKPG+
Sbjct: 361 QLKTQSRPSSPYPN------------YPDLKPPSSPMPSKPGK 366

BLAST of Spg014599 vs. TAIR 10
Match: AT3G25480.1 (Rhodanese/Cell cycle control phosphatase superfamily protein )

HSP 1 Score: 233.0 bits (593), Expect = 4.1e-61
Identity = 136/280 (48.57%), Postives = 177/280 (63.21%), Query Frame = 0

Query: 55  TPLPNPTNP------SKPLQNPQFLFSKTSSFHELAKTHLPFALTQLLLTPLPGFALE-- 114
           +PL N T P       KP+QN     +K    H L KT+L   ++QL++T  P  A E  
Sbjct: 11  SPLRNLTKPCSTSQIPKPIQNS----TKQPPIHLLTKTNLSVTISQLIITS-PVLASESF 70

Query: 115 ----ADQASDKINLESILLSIDDFFNRYPFFVAGCTFIWLVGIPLIEYFFRKYKFVSAID 174
               +D +S KI+LESIL++ID+FFN+YPFFVAGCTF +LV  P + ++ RKYK +SA++
Sbjct: 71  DSISSDPSSGKIDLESILVTIDNFFNKYPFFVAGCTFTYLVVYPAVMFYLRKYKPISAMN 130

Query: 175 AFRKIRDDPNAQLLDIRDEKSLAFLGSPNLRILNKDVVQVVYSEEDEDGFVKKVKKSLGN 234
           AFRK++++ ++QLLDIRD K+LA L SPNL+ L K  VQV +SE DE+GF+ KVK S  +
Sbjct: 131 AFRKLKNESDSQLLDIRDVKTLALLASPNLKFLGKSSVQVPFSENDEEGFLTKVKGSFSD 190

Query: 235 GADTVVFVLDNFDGNSMKVAELLFKNGFKEAYAIKDGVRGEKGWLDLVLLYGFSSTTAQG 294
             +TVV VLDNFDGNS KVAELL KNGFKEAY I+ G RG+ GWL               
Sbjct: 191 AENTVVCVLDNFDGNSSKVAELLIKNGFKEAYYIRGGARGKNGWL--------------- 250

Query: 295 HKLPPNAIQESLLPPSVHMNRRRKAKASQKFGTNGVVQQN 323
                 AIQE LLPP VHM   +  K+S     + V  +N
Sbjct: 251 ------AIQEELLPPPVHMYTAKNVKSSNNNEASVVGTEN 264

BLAST of Spg014599 vs. TAIR 10
Match: AT4G01050.1 (thylakoid rhodanese-like )

HSP 1 Score: 115.5 bits (288), Expect = 9.5e-26
Identity = 78/234 (33.33%), Postives = 113/234 (48.29%), Query Frame = 0

Query: 40  PLSVFNGAHFSSINKTPLPN--PTNPSKPLQNPQFLFSKTSSFHELAKTHLPFALTQLLL 99
           P+SV +           LPN  P    +P+    FL  + +    L  + L  A      
Sbjct: 11  PMSVLSEKRSEPRKPFSLPNLFPPKSQRPISQESFL-KRFNGGLALLTSVLSSATAPAKS 70

Query: 100 TPLPGFALEADQASDKINLESILLSIDDFFNRYPFFVAGCTFIWLVGIPLIEYFFRKYK- 159
                   ++   S   + + ++  I +F    P  +AG      V   L +   +K K 
Sbjct: 71  LTYEEALQQSMTTSSSFDSDGLIEGISNFVTDNPLVIAGGVAALAVPFVLSQVLNKKPKS 130

Query: 160 --FVSAIDAFRKIRDDPNAQLLDIRDEKSLAFLGSPNLRILNKDVVQVVYSEEDEDGFVK 219
               SA +A+ K+  D NAQLLDIR       +GSPN++ L K  V  VY+ ED+ GF+K
Sbjct: 131 WGVESAKNAYTKLGTDDNAQLLDIRATADFRQVGSPNIKGLGKKAVSTVYNGEDKPGFLK 190

Query: 220 KVKKSLGNGADTVVFVLDNFDGNSMKVAELLFKNGFKEAYAIKDGVRGEKGWLD 269
           K+     +  +T +++LD FDGNS  VAEL+  NGFK AYAIKDG  G +GWL+
Sbjct: 191 KLSLKFKDPENTTLYILDKFDGNSELVAELVALNGFKSAYAIKDGAEGPRGWLN 243

BLAST of Spg014599 vs. TAIR 10
Match: AT5G23060.1 (calcium sensing receptor )

HSP 1 Score: 42.7 bits (99), Expect = 7.8e-04
Identity = 41/154 (26.62%), Postives = 64/154 (41.56%), Query Frame = 0

Query: 135 AGCTFIWLVGIP----LIEYFFRKYKFVSAIDAFRKIRDDPNAQLLDIRDEKSLAFLGSP 194
           AG  F+  + +P     I + FR YK          +    N  ++DIR EK     G P
Sbjct: 193 AGAAFLAYLLLPPVFSAISFNFRGYKGDLTPAQTLDLLCTKNYLMVDIRSEKDKEKAGIP 252

Query: 195 NLRILNKDVVQVVYSEE---DEDGFVKKVK--------------KSLGNGADTVVFVLDN 254
            L    K+ V  +  EE      G V+  K              K +  G++  + +LD+
Sbjct: 253 RLPSNAKNRVISIPLEELPNKVKGIVRNSKRVEAEIAALKISYLKKINKGSN--IIILDS 312

Query: 255 FDGNSMKVAELLFKNGFKEAYAIKDGVRGEKGWL 268
           +  ++  VA+ L   G+K  Y + DG  G +GWL
Sbjct: 313 YTDSAKIVAKTLKVLGYKNCYIVTDGFSGGRGWL 344