Spg014353 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg014353
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionRibonuclease T(2)
Locationscaffold3: 38596950 .. 38598155 (+)
RNA-Seq ExpressionSpg014353
SyntenySpg014353
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCATCGCAAAATATATATACTGTCCATTCTGCGCGACAGCGTCGAGACGCTAAGGACACAGCATCGCGACGCTGTCGCGCGCGTGCCCTAATTATTTCAGGAAAGTTCGCAGCGTCGAGACGCTGTGGAGGTAGCGTCTCGACGCTGCCTCGAATTCGCGCCTCCTGCTGAAGGGGCCAGCGTTGAGACTCTCCAATGGTAGCGTCTCAACGCTGCCCTATTATATGGATGCCTTGAACCTCATGTTCTTTTGCAAATTCCTTTTCTTGGGTTAATTCTTTTCCTCCATAATTAACGGTATTCTTCAAAAACATCAATTTCCATTGAAATGCTTGATTCCTACAAAACAAAACCAAATAGACATAAAATTGGCCAAATAGTACCCAAGACTAACGCTAAAATAATATAAAATATAGCTCAATAAATTGCGCTATCAAAGGGTAAGGGCTCGCTTTAGTATGATTATGCATTGAATTTATAATTTCTTGCTTTTTTGATAGATGGCTTATGGGCTGATTATAATGATGGAACATGGCCTGCATGTTGCACTGGAACGAATTTTGATGAGAAGGAGGTACGTTTTTATTAAGATTAGGCTGTTGATATTCATGAAATGATCCAGAGCTGAATTTTTTTAGTGGGTAGAAGTTTGGTGCCAAAGGTCAAAGAGTGTAGAAACTTTTGGTATCAAAAGGTATATAAAAAAGAGCCAAAATTGGATGAGGTTCATGGGATCTAGAAAAGTTCCCCTATTTCTTTCAAATGATCAAATAGCTGTAACTATGCTAGGATTTTGGCTTTGTTCCAAAACTTTCCAAAACTTGTGATCCTCAGAAACTTAAACCATGAAACCCCTGAACCCTATGGGAAAGCATTATGCAAGTTGAAGAGCTAAACAAAACCCTTTCGGCCTGATTTTCATATCTTACTGTTGGTTTTACTTTGATCATTGTGCCATTTACTTTGTTCTTGCAATTGATAGATACAATGTTCAGCATAAGGCATTCTTGTAACATCCTGCATCATTTAATCTTGCAGATTCGTACCTTACTTGAACCCCTGAAGAAATATTGGCCATCTTTGAGTTGTAGTTCACCGTCAACTTGCCATGGTGCAAAAGGATCATTTTGGGCTCATGAGGTGACCGTCACTAGTACAAGATATATTTGGTTCAAAATTGCCCTGATGATACTCAAGTTTTAA

mRNA sequence

ATGGCATCGCAAAATATATATACTGTCCATTCTGCGCGACAGCGTCGAGACGCTAAGGACACAGCATCGCGACGCTGTCGCGCGCGTGCCCTAATTATTTCAGGAAAGTTCGCAGCGTCGAGACGCTGTGGAGATGGCTTATGGGCTGATTATAATGATGGAACATGGCCTGCATGTTGCACTGGAACGAATTTTGATGAGAAGGAGATTCGTACCTTACTTGAACCCCTGAAGAAATATTGGCCATCTTTGAGTTGTAGTTCACCGTCAACTTGCCATGGTGCAAAAGGATCATTTTGGGCTCATGAGGTGACCGTCACTAGTACAAGATATATTTGGTTCAAAATTGCCCTGATGATACTCAAGTTTTAA

Coding sequence (CDS)

ATGGCATCGCAAAATATATATACTGTCCATTCTGCGCGACAGCGTCGAGACGCTAAGGACACAGCATCGCGACGCTGTCGCGCGCGTGCCCTAATTATTTCAGGAAAGTTCGCAGCGTCGAGACGCTGTGGAGATGGCTTATGGGCTGATTATAATGATGGAACATGGCCTGCATGTTGCACTGGAACGAATTTTGATGAGAAGGAGATTCGTACCTTACTTGAACCCCTGAAGAAATATTGGCCATCTTTGAGTTGTAGTTCACCGTCAACTTGCCATGGTGCAAAAGGATCATTTTGGGCTCATGAGGTGACCGTCACTAGTACAAGATATATTTGGTTCAAAATTGCCCTGATGATACTCAAGTTTTAA

Protein sequence

MASQNIYTVHSARQRRDAKDTASRRCRARALIISGKFAASRRCGDGLWADYNDGTWPACCTGTNFDEKEIRTLLEPLKKYWPSLSCSSPSTCHGAKGSFWAHEVTVTSTRYIWFKIALMILKF
Homology
BLAST of Spg014353 vs. NCBI nr
Match: TYK17921.1 (ribonuclease 2-like [Cucumis melo var. makuwa])

HSP 1 Score: 130.2 bits (326), Expect = 1.2e-26
Identity = 53/58 (91.38%), Postives = 55/58 (94.83%), Query Frame = 0

Query: 46  GLWADYNDGTWPACCTGTNFDEKEIRTLLEPLKKYWPSLSCSSPSTCHGAKGSFWAHE 104
           GLW DYNDGTWPACC G NFDEKEIRTLL+PLKKYWPSLSCSSPSTCHG+KGSFWAHE
Sbjct: 84  GLWPDYNDGTWPACCNGKNFDEKEIRTLLDPLKKYWPSLSCSSPSTCHGSKGSFWAHE 141

BLAST of Spg014353 vs. NCBI nr
Match: KAA0041985.1 (ribonuclease 2-like [Cucumis melo var. makuwa])

HSP 1 Score: 130.2 bits (326), Expect = 1.2e-26
Identity = 53/58 (91.38%), Postives = 55/58 (94.83%), Query Frame = 0

Query: 46  GLWADYNDGTWPACCTGTNFDEKEIRTLLEPLKKYWPSLSCSSPSTCHGAKGSFWAHE 104
           GLW DYNDGTWPACC G NFDEKEIRTLL+PLKKYWPSLSCSSPSTCHG+KGSFWAHE
Sbjct: 84  GLWPDYNDGTWPACCNGKNFDEKEIRTLLDPLKKYWPSLSCSSPSTCHGSKGSFWAHE 141

BLAST of Spg014353 vs. NCBI nr
Match: KAG6593061.1 (Ribonuclease 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 127.1 bits (318), Expect = 1.0e-25
Identity = 53/64 (82.81%), Postives = 55/64 (85.94%), Query Frame = 0

Query: 46  GLWADYNDGTWPACCTGTNFDEKEIRTLLEPLKKYWPSLSCSSPSTCHGAKGSFWAHEVT 105
           GLW DYNDGTWP+CCTG  FDEKEI TLLEP+KKYW SLSCSSP TCHGAKGSFWAHEVT
Sbjct: 106 GLWPDYNDGTWPSCCTGKEFDEKEIHTLLEPMKKYWSSLSCSSPKTCHGAKGSFWAHEVT 165

Query: 106 VTST 110
              T
Sbjct: 166 KHGT 169

BLAST of Spg014353 vs. NCBI nr
Match: XP_008447208.1 (PREDICTED: ribonuclease 2-like [Cucumis melo])

HSP 1 Score: 126.7 bits (317), Expect = 1.4e-25
Identity = 52/58 (89.66%), Postives = 54/58 (93.10%), Query Frame = 0

Query: 46  GLWADYNDGTWPACCTGTNFDEKEIRTLLEPLKKYWPSLSCSSPSTCHGAKGSFWAHE 104
           GLW DYNDGTWPACC G  FDEKEIRTLL+PLKKYWPSLSCSSPSTCHG+KGSFWAHE
Sbjct: 84  GLWPDYNDGTWPACCNGKIFDEKEIRTLLDPLKKYWPSLSCSSPSTCHGSKGSFWAHE 141

BLAST of Spg014353 vs. NCBI nr
Match: KGN45175.2 (hypothetical protein Csa_016228 [Cucumis sativus])

HSP 1 Score: 126.7 bits (317), Expect = 1.4e-25
Identity = 54/68 (79.41%), Postives = 59/68 (86.76%), Query Frame = 0

Query: 37  FAASRRC-GDGLWADYNDGTWPACCTGTNFDEKEIRTLLEPLKKYWPSLSCSSPSTCHGA 96
           F ++ RC  +GLW DYNDGTWPACC G  FDEKEI TLL+PLKKYWPSLSCSSPSTCHG+
Sbjct: 245 FESNSRCYANGLWPDYNDGTWPACCNGKIFDEKEILTLLDPLKKYWPSLSCSSPSTCHGS 304

Query: 97  KGSFWAHE 104
           KGSFWAHE
Sbjct: 305 KGSFWAHE 312

BLAST of Spg014353 vs. ExPASy Swiss-Prot
Match: P42814 (Ribonuclease 2 OS=Arabidopsis thaliana OX=3702 GN=RNS2 PE=2 SV=1)

HSP 1 Score: 105.5 bits (262), Expect = 4.3e-22
Identity = 44/81 (54.32%), Postives = 58/81 (71.60%), Query Frame = 0

Query: 23  SRRCRARALIISGKFAASRRCGDGLWADYNDGTWPACCTGTNFDEKEIRTLLEPLKKYWP 82
           +R C ++     G  A ++    GLW DYNDG+WP+CC  ++F EKEI TL++ L+KYWP
Sbjct: 49  TRHCCSKNACCRGSDAPTQFTIHGLWPDYNDGSWPSCCYRSDFKEKEISTLMDGLEKYWP 108

Query: 83  SLSCSSPSTCHGAKGSFWAHE 104
           SLSC SPS+C+G KGSFW HE
Sbjct: 109 SLSCGSPSSCNGGKGSFWGHE 129

BLAST of Spg014353 vs. ExPASy Swiss-Prot
Match: P42813 (Ribonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=RNS1 PE=1 SV=1)

HSP 1 Score: 56.6 bits (135), Expect = 2.3e-07
Identity = 26/59 (44.07%), Postives = 35/59 (59.32%), Query Frame = 0

Query: 46  GLWADYNDGTWPACCTGTN-FDEKEIRTLLEPLKKYWPSLSCSSPSTCHGAKGSFWAHE 104
           GLW +Y DGT+P+ C  +  FD   I  LL  +KK WP+L+C S     G+  +FW HE
Sbjct: 66  GLWPNYKDGTYPSNCDASKPFDSSTISDLLTSMKKSWPTLACPS-----GSGEAFWEHE 119

BLAST of Spg014353 vs. ExPASy Swiss-Prot
Match: P80022 (Extracellular ribonuclease LE OS=Solanum lycopersicum OX=4081 PE=1 SV=2)

HSP 1 Score: 54.3 bits (129), Expect = 1.1e-06
Identity = 27/82 (32.93%), Postives = 48/82 (58.54%), Query Frame = 0

Query: 46  GLWADYNDGTWPA-CCTGTNFDEKEIRTLLEPLKKYWPSLSCSSPSTCHGAKGSFWAHE- 105
           GLW + NDGT+P+ C   + +D+ +I  L+  +++ WP+L+C S     G+  +FW+HE 
Sbjct: 65  GLWPNNNDGTYPSNCDPNSPYDQSQISDLISSMQQNWPTLACPS-----GSGSTFWSHEW 124

Query: 106 -------VTVTSTRYIWFKIAL 119
                   +V + ++ +FK AL
Sbjct: 125 EKHGTCAESVLTNQHAYFKKAL 141

BLAST of Spg014353 vs. ExPASy TrEMBL
Match: A0A5A7TKN5 (Ribonuclease T(2) OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold67G004250 PE=3 SV=1)

HSP 1 Score: 130.2 bits (326), Expect = 6.0e-27
Identity = 53/58 (91.38%), Postives = 55/58 (94.83%), Query Frame = 0

Query: 46  GLWADYNDGTWPACCTGTNFDEKEIRTLLEPLKKYWPSLSCSSPSTCHGAKGSFWAHE 104
           GLW DYNDGTWPACC G NFDEKEIRTLL+PLKKYWPSLSCSSPSTCHG+KGSFWAHE
Sbjct: 84  GLWPDYNDGTWPACCNGKNFDEKEIRTLLDPLKKYWPSLSCSSPSTCHGSKGSFWAHE 141

BLAST of Spg014353 vs. ExPASy TrEMBL
Match: A0A5D3D100 (Ribonuclease T(2) OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold306G002160 PE=3 SV=1)

HSP 1 Score: 130.2 bits (326), Expect = 6.0e-27
Identity = 53/58 (91.38%), Postives = 55/58 (94.83%), Query Frame = 0

Query: 46  GLWADYNDGTWPACCTGTNFDEKEIRTLLEPLKKYWPSLSCSSPSTCHGAKGSFWAHE 104
           GLW DYNDGTWPACC G NFDEKEIRTLL+PLKKYWPSLSCSSPSTCHG+KGSFWAHE
Sbjct: 84  GLWPDYNDGTWPACCNGKNFDEKEIRTLLDPLKKYWPSLSCSSPSTCHGSKGSFWAHE 141

BLAST of Spg014353 vs. ExPASy TrEMBL
Match: A0A1S3BHR4 (Ribonuclease T(2) OS=Cucumis melo OX=3656 GN=LOC103489711 PE=3 SV=1)

HSP 1 Score: 126.7 bits (317), Expect = 6.6e-26
Identity = 52/58 (89.66%), Postives = 54/58 (93.10%), Query Frame = 0

Query: 46  GLWADYNDGTWPACCTGTNFDEKEIRTLLEPLKKYWPSLSCSSPSTCHGAKGSFWAHE 104
           GLW DYNDGTWPACC G  FDEKEIRTLL+PLKKYWPSLSCSSPSTCHG+KGSFWAHE
Sbjct: 84  GLWPDYNDGTWPACCNGKIFDEKEIRTLLDPLKKYWPSLSCSSPSTCHGSKGSFWAHE 141

BLAST of Spg014353 vs. ExPASy TrEMBL
Match: A0A0A0K5E4 (Ribonuclease T(2) OS=Cucumis sativus OX=3659 GN=Csa_7G374550 PE=3 SV=1)

HSP 1 Score: 124.0 bits (310), Expect = 4.3e-25
Identity = 51/58 (87.93%), Postives = 53/58 (91.38%), Query Frame = 0

Query: 46  GLWADYNDGTWPACCTGTNFDEKEIRTLLEPLKKYWPSLSCSSPSTCHGAKGSFWAHE 104
           GLW DYNDGTWPACC G  FDEKEI TLL+PLKKYWPSLSCSSPSTCHG+KGSFWAHE
Sbjct: 84  GLWPDYNDGTWPACCNGKIFDEKEILTLLDPLKKYWPSLSCSSPSTCHGSKGSFWAHE 141

BLAST of Spg014353 vs. ExPASy TrEMBL
Match: A0A6J1KXE3 (Ribonuclease T(2) OS=Cucurbita maxima OX=3661 GN=LOC111498005 PE=3 SV=1)

HSP 1 Score: 123.6 bits (309), Expect = 5.6e-25
Identity = 50/58 (86.21%), Postives = 52/58 (89.66%), Query Frame = 0

Query: 46  GLWADYNDGTWPACCTGTNFDEKEIRTLLEPLKKYWPSLSCSSPSTCHGAKGSFWAHE 104
           GLW DYNDGTWP+CCTG  FDEKEI TLLEP+KKYW SLSC SPSTCHGAKGSFWAHE
Sbjct: 91  GLWPDYNDGTWPSCCTGKEFDEKEIHTLLEPMKKYWSSLSCGSPSTCHGAKGSFWAHE 148

BLAST of Spg014353 vs. TAIR 10
Match: AT2G39780.2 (ribonuclease 2 )

HSP 1 Score: 107.1 bits (266), Expect = 1.0e-23
Identity = 45/90 (50.00%), Postives = 61/90 (67.78%), Query Frame = 0

Query: 23  SRRCRARALIISGKFAASRRCGDGLWADYNDGTWPACCTGTNFDEKEIRTLLEPLKKYWP 82
           +R C ++     G  A ++    GLW DYNDG+WP+CC  ++F EKEI TL++ L+KYWP
Sbjct: 49  TRHCCSKNACCRGSDAPTQFTIHGLWPDYNDGSWPSCCYRSDFKEKEISTLMDGLEKYWP 108

Query: 83  SLSCSSPSTCHGAKGSFWAHEVTVTSTRYI 113
           SLSC SPS+C+G KGSFW HE  +    Y+
Sbjct: 109 SLSCGSPSSCNGGKGSFWGHEDVLYQAGYV 138

BLAST of Spg014353 vs. TAIR 10
Match: AT2G39780.1 (ribonuclease 2 )

HSP 1 Score: 105.5 bits (262), Expect = 3.0e-23
Identity = 44/81 (54.32%), Postives = 58/81 (71.60%), Query Frame = 0

Query: 23  SRRCRARALIISGKFAASRRCGDGLWADYNDGTWPACCTGTNFDEKEIRTLLEPLKKYWP 82
           +R C ++     G  A ++    GLW DYNDG+WP+CC  ++F EKEI TL++ L+KYWP
Sbjct: 49  TRHCCSKNACCRGSDAPTQFTIHGLWPDYNDGSWPSCCYRSDFKEKEISTLMDGLEKYWP 108

Query: 83  SLSCSSPSTCHGAKGSFWAHE 104
           SLSC SPS+C+G KGSFW HE
Sbjct: 109 SLSCGSPSSCNGGKGSFWGHE 129

BLAST of Spg014353 vs. TAIR 10
Match: AT1G14220.1 (Ribonuclease T2 family protein )

HSP 1 Score: 56.6 bits (135), Expect = 1.6e-08
Identity = 33/78 (42.31%), Postives = 41/78 (52.56%), Query Frame = 0

Query: 27  RARALIISGKFAASRRCGDGLWADYNDGTWPA-CCTGTNFDEKEIRTLLEPLKKYWPSLS 86
           RA     SGK AA      GLW +YN G+WP+ C   + FD  +I  L+  LKK WP+LS
Sbjct: 46  RACCYPTSGKPAADFGI-HGLWPNYNGGSWPSNCDPDSQFDRSQISDLVSSLKKNWPTLS 105

Query: 87  CSSPSTCHGAKGSFWAHE 104
           C S         +FW HE
Sbjct: 106 CPS-----NEGFNFWEHE 117

BLAST of Spg014353 vs. TAIR 10
Match: AT2G02990.1 (ribonuclease 1 )

HSP 1 Score: 56.6 bits (135), Expect = 1.6e-08
Identity = 26/59 (44.07%), Postives = 35/59 (59.32%), Query Frame = 0

Query: 46  GLWADYNDGTWPACCTGTN-FDEKEIRTLLEPLKKYWPSLSCSSPSTCHGAKGSFWAHE 104
           GLW +Y DGT+P+ C  +  FD   I  LL  +KK WP+L+C S     G+  +FW HE
Sbjct: 66  GLWPNYKDGTYPSNCDASKPFDSSTISDLLTSMKKSWPTLACPS-----GSGEAFWEHE 119

BLAST of Spg014353 vs. TAIR 10
Match: AT1G14210.1 (Ribonuclease T2 family protein )

HSP 1 Score: 45.8 bits (107), Expect = 2.9e-05
Identity = 22/59 (37.29%), Postives = 31/59 (52.54%), Query Frame = 0

Query: 46  GLWADYNDGTWPACCTGTN-FDEKEIRTLLEPLKKYWPSLSCSSPSTCHGAKGSFWAHE 104
           GLW  +N+GTWPA C  TN FD  +I  L+  ++K W      +   C   + + W HE
Sbjct: 62  GLWPQFNNGTWPAFCDQTNLFDISKISDLVCQMEKKWTEWGVWA---CPSNETNLWEHE 117

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
TYK17921.11.2e-2691.38ribonuclease 2-like [Cucumis melo var. makuwa][more]
KAA0041985.11.2e-2691.38ribonuclease 2-like [Cucumis melo var. makuwa][more]
KAG6593061.11.0e-2582.81Ribonuclease 2, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_008447208.11.4e-2589.66PREDICTED: ribonuclease 2-like [Cucumis melo][more]
KGN45175.21.4e-2579.41hypothetical protein Csa_016228 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
P428144.3e-2254.32Ribonuclease 2 OS=Arabidopsis thaliana OX=3702 GN=RNS2 PE=2 SV=1[more]
P428132.3e-0744.07Ribonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=RNS1 PE=1 SV=1[more]
P800221.1e-0632.93Extracellular ribonuclease LE OS=Solanum lycopersicum OX=4081 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A5A7TKN56.0e-2791.38Ribonuclease T(2) OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold67G004... [more]
A0A5D3D1006.0e-2791.38Ribonuclease T(2) OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold306G00... [more]
A0A1S3BHR46.6e-2689.66Ribonuclease T(2) OS=Cucumis melo OX=3656 GN=LOC103489711 PE=3 SV=1[more]
A0A0A0K5E44.3e-2587.93Ribonuclease T(2) OS=Cucumis sativus OX=3659 GN=Csa_7G374550 PE=3 SV=1[more]
A0A6J1KXE35.6e-2586.21Ribonuclease T(2) OS=Cucurbita maxima OX=3661 GN=LOC111498005 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT2G39780.21.0e-2350.00ribonuclease 2 [more]
AT2G39780.13.0e-2354.32ribonuclease 2 [more]
AT1G14220.11.6e-0842.31Ribonuclease T2 family protein [more]
AT2G02990.11.6e-0844.07ribonuclease 1 [more]
AT1G14210.12.9e-0537.29Ribonuclease T2 family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001568Ribonuclease T2-likePFAMPF00445Ribonuclease_T2coord: 45..103
e-value: 1.5E-8
score: 35.0
IPR001568Ribonuclease T2-likePANTHERPTHR11240RIBONUCLEASE T2coord: 23..106
IPR036430Ribonuclease T2-like superfamilyGENE3D3.90.730.10coord: 12..116
e-value: 4.7E-17
score: 64.7
IPR036430Ribonuclease T2-like superfamilySUPERFAMILY55895Ribonuclease Rh-likecoord: 45..103
NoneNo IPR availablePANTHERPTHR11240:SF53RIBONUCLEASE 2-LIKE ISOFORM X1coord: 23..106

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg014353.1Spg014353.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006777 Mo-molybdopterin cofactor biosynthetic process
biological_process GO:0006401 RNA catabolic process
biological_process GO:0090502 RNA phosphodiester bond hydrolysis, endonucleolytic
cellular_component GO:0005576 extracellular region
cellular_component GO:0019008 molybdopterin synthase complex
molecular_function GO:0051539 4 iron, 4 sulfur cluster binding
molecular_function GO:0061798 GTP 3',8'-cyclase activity
molecular_function GO:0005525 GTP binding
molecular_function GO:0046872 metal ion binding
molecular_function GO:0033897 ribonuclease T2 activity
molecular_function GO:0003723 RNA binding