Spg014229 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg014229 vs. NCBI nr
Match: KAG7014111.1 (putative 2-oxoglutarate-dependent dioxygenase AOP1.2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 673.3 bits (1736), Expect = 1.8e-189
Identity = 348/600 (58.00%), Postives = 409/600 (68.17%), Query Frame = 0

Query: 1   MGSETPLMRTEELPVINFSGGDLKAEDGGEWRWTSKKVREALEEYGCFIAVYDRVSSELR 60
           MGSET       LPVINFSG D+KAE G  WR  S +VREALEEYGCFIA Y+ +S ELR
Sbjct: 29  MGSETGPRMDAGLPVINFSGDDVKAEGGEGWRRRSGEVREALEEYGCFIAEYEGISPELR 88

Query: 61  GEIFKALKALFDLPVETKKKNKYEKPFNGYVGQLRTLPLHESLGIDDATNFHGVQSFANL 120
            E+FKA++ALF+LP+ETKKKN+YEKPFNGYVG+LRTLPLHESLGIDDATN          
Sbjct: 89  VEVFKAVEALFELPIETKKKNRYEKPFNGYVGELRTLPLHESLGIDDATNI--------- 148

Query: 121 MWPSGNHHFSETLHSFAKIAEELDQMVTKMIFESYGVENYHHSHASSITYLLRVLKNKAP 180
                                               VE YH +HA+SITYLLR LKNKAP
Sbjct: 149 -----------------------------------DVEKYHETHAASITYLLRALKNKAP 208

Query: 181 QSENPSLCLVDHTDKSFTTMVYQDHTNGLEVQTKDGQWIKVEISSPANFVVIAGDAFQ-- 240
            S+NP+LCLVDHTDKSFTTMVYQDHTNGLEVQTKDG+WI+V+ SSP+ FV  + D  +  
Sbjct: 209 HSQNPTLCLVDHTDKSFTTMVYQDHTNGLEVQTKDGRWIQVDNSSPSTFVAWSNDRIESP 268

Query: 241 ---LVIMGSQAKAKIPTI--------------------------------------DLSK 300
              + I G++ +  +                                         DLSK
Sbjct: 269 THKVTIRGNEDRYSLAMFGFSNGIVEVPKELIDQTHPLRYKPFDHIGLLHFFRSKEDLSK 328

Query: 301 EKLKAAGTEEWALSCKEMRVALEEFGCFLVVYDEVSLELHNSIFEATKELFDLPLESRIQ 360
            +   AG++ W  +CK MRV LEEFGCF VV++EVSLELHNS F A  ELF LP+E+RI+
Sbjct: 329 LEKLEAGSDAWGSTCKAMRVGLEEFGCFCVVFEEVSLELHNSFFGAVDELFQLPIETRIK 388

Query: 361 NTSEKPYHGYFGRYSFLPLYESMAIDNPSDLQAAQAFANLMWP-AGNNHFCETVQNFSTM 420
           NTSEK YHGYFGRYSFLPLYESMAIDNP+ L AAQ+FANLMWP  GN+ F          
Sbjct: 389 NTSEKAYHGYFGRYSFLPLYESMAIDNPTQLDAAQSFANLMWPDQGNDRF---------- 448

Query: 421 VAKLDEMVTRMVFESYGVSK-LSDSNIESTNHLLRCFKYRVPEKDETDLGLHSHTDLTFF 480
                         SYGVSK L D+N++ST +LLR FKYRVPE+ ETD+GLH+HTDLTFF
Sbjct: 449 --------------SYGVSKQLIDTNVDSTANLLRFFKYRVPEQHETDVGLHAHTDLTFF 508

Query: 481 SIIHQHLIGGLQIQSLDDEWIEVEPSGHCSFIVMAGDAIMAWSNERIRACRHRVIMRGNE 540
           SIIHQHLIGGLQIQSLDD+WI++EPS HCSFIVMAGDA+MAWSN+RIR+CRHRV M+  E
Sbjct: 509 SIIHQHLIGGLQIQSLDDQWIDLEPSSHCSFIVMAGDALMAWSNDRIRSCRHRVTMKAEE 560

Query: 541 RRYSVGLFSFKSGTIGVPEELVDDANPLRYKPFDHFQFLSYDKANASHKTVSRIKDYCGL 556
            RYS G+FSFK+GTI VPEELVDDANPLRYKPF+H+ FL++D A  S K + RI+DYC +
Sbjct: 569 TRYSAGMFSFKNGTISVPEELVDDANPLRYKPFNHYDFLTHDIAKGSKKFLYRIRDYCSI 560

BLAST of Spg014229 vs. NCBI nr
Match: RXH69044.1 (hypothetical protein DVH24_031377 [Malus domestica])

HSP 1 Score: 573.9 bits (1478), Expect = 1.5e-159
Identity = 299/632 (47.31%), Postives = 402/632 (63.61%), Query Frame = 0

Query: 1   MGSETPLMRTEELPVINFSGGDLKAEDGGEWRWTSKKVREALEEYGCFIAVYDRVSSELR 60
           MGSE      ++LPV++FS   LK      W    K V+ ALEE+GCF+ +YD++S E R
Sbjct: 20  MGSEV----EQKLPVLDFSKEALK-PGTNSWLLACKDVQRALEEFGCFVVLYDKISDEFR 79

Query: 61  GEIFKALKALFDLPVETKKKNKYEKPFNGYVGQLRTLPLHESLGIDDATNFHGVQSFANL 120
                +L+ LFDLP ETK  NKYEKP NGYVGQ+  LPLHESLGID ATNF   + F  L
Sbjct: 80  NAFVGSLEELFDLPTETKMTNKYEKPLNGYVGQIPKLPLHESLGIDCATNFEETKKFTKL 139

Query: 121 MWPSGNHHFSETLHSFAKIAEELDQMVTKMIFESYGVENYHHSHASSITYLLRVLKNKAP 180
           +         E  ++FAK+AEELDQMV +MIFESYGVE Y+ S+  SI+YLLR+L+N++P
Sbjct: 140 I---------ERAYAFAKVAEELDQMVARMIFESYGVEKYYDSYVGSISYLLRILQNRSP 199

Query: 181 QSENPSLCLVDHTDKSFTTMVYQDH-TNGLEVQTKDGQWIKVEISSPANFVVIAGDAFQL 240
           +    +L  V HTDKSFTT++YQ++  N LEV+T++ +WIKVE   P++F+V+AGDA   
Sbjct: 200 KENEHNLGFVAHTDKSFTTVLYQNNQVNALEVETRNHEWIKVEF-PPSSFIVMAGDALMA 259

Query: 241 ------------VI---------------------------------------------- 300
                       VI                                              
Sbjct: 260 WSNDRILSPNHRVIMSGNETRYSLAQFAFSDGEIHVPEELGDEERPLRYKSFDHPGLLRF 319

Query: 301 -------------------MGSQAKAKIPTIDLSKEKLKAAGTEEWALSCKEMRVALEEF 360
                              MGS+ + K+P IDLS E LK  GT+ W L+CKE++ ALEE+
Sbjct: 320 FRSQDRYISNSAIKAYCDRMGSETQPKLPVIDLSDENLK-PGTDAWLLACKEIKDALEEY 379

Query: 361 GCFLVVYDEVSLELHNSIFEATKELFDLPLESRIQNTSEKPYHGYFGRYSFLPLYESMAI 420
           GCF  +Y ++ LELHNSIF A ++LF LPLE++ Q TS++PYH YFG+YSFLPLYES+ +
Sbjct: 380 GCFEAIYHKIPLELHNSIFSAMEDLFGLPLETKKQKTSDRPYHSYFGQYSFLPLYESLGV 439

Query: 421 DNPSDLQAAQAFANLMWPAGNNHFCETVQNFSTMVAKLDEMVTRMVFESYGVSKLSDSNI 480
           DNP+ ++ AQ F +++         ET  +FS +VA+L +MVTRMVF+ YGV +L DS++
Sbjct: 440 DNPTTVEGAQGFTSII---------ETAHSFSKLVAELSQMVTRMVFDMYGVQRLFDSHM 499

Query: 481 ESTNHLLRCFKYRVPEKDETDLGLHSHTDLTFFSIIHQHLIGGLQIQSLDDEWIEVEPSG 540
           EST +LLRCFKYR  E++ET++GLH HTD TF SI++Q+ + GLQI + D +WI+V PS 
Sbjct: 500 ESTTYLLRCFKYRTQEENETNVGLHPHTDKTFISILNQNQVNGLQIGTKDGQWIDVNPS- 559

Query: 541 HCSFIVMAGDAIMAWSNERIRACRHRVIMRGNERRYSVGLFSFKSGTIGVPEELVDDANP 555
             SF+++AGDA MAWSN+R+ +C H+V M+ N+ RYS+GLF+FKSG I VPEEL+D+ +P
Sbjct: 560 PSSFLILAGDAFMAWSNDRVPSCDHQVTMKENKTRYSLGLFAFKSGIIQVPEELIDEKHP 619

BLAST of Spg014229 vs. NCBI nr
Match: XP_022145687.1 (probable 2-oxoglutarate-dependent dioxygenase AOP1 [Momordica charantia])

HSP 1 Score: 525.0 bits (1351), Expect = 7.8e-145
Identity = 250/319 (78.37%), Postives = 287/319 (89.97%), Query Frame = 0

Query: 242 MGSQAKAKIPTIDLSKEKLKAAGTEEWALSCKEMRVALEEFGCFLVVYDEVSLELHNSIF 301
           MG+QAK+KIPTI+LS+EKLK  G+EEW L  KE+RVALEEFGCFLVVY++VSLELHNSIF
Sbjct: 1   MGTQAKSKIPTIELSEEKLK-VGSEEWVLIGKEIRVALEEFGCFLVVYEKVSLELHNSIF 60

Query: 302 EATKELFDLPLESRIQNTSEKPYHGYFGRYSFLPLYESMAIDNPSDLQAAQAFANLMWPA 361
           EATKELF LP+E+RIQNTSEKPYHGYFGRYSFLPLYESMA+DNP+ LQA Q F NLMWPA
Sbjct: 61  EATKELFHLPIETRIQNTSEKPYHGYFGRYSFLPLYESMAVDNPTSLQAIQTFTNLMWPA 120

Query: 362 GNNHFCETVQNFSTMVAKLDEMVTRMVFESYGVSK-LSDSNIESTNHLLRCFKYRVPEKD 421
           GN  FCETV +FST+VAKL+E VTRMV ESYGVSK + +S+IEST HLLRCFKYRVP++D
Sbjct: 121 GNKTFCETVHDFSTLVAKLEETVTRMVLESYGVSKQVCESSIESTTHLLRCFKYRVPKQD 180

Query: 422 ETDLGLHSHTDLTFFSIIHQHLIGGLQIQSLDD----EWIEVEPSGHCSFIVMAGDAIMA 481
           ETDLGLHSHTDLTF SI+HQHLIGGLQ++SLD+    EWI+++P G  SFIVMAGDA+MA
Sbjct: 181 ETDLGLHSHTDLTFLSILHQHLIGGLQVKSLDNNNDGEWIDIDPVGRSSFIVMAGDALMA 240

Query: 482 WSNERIRACRHRVIMRGNERRYSVGLFSFKSGTIGVPEELVDDANPLRYKPFDHFQFLSY 541
           WSN+RIR CRHRV+MRG+E RYS+GLFSFKSGTI VPEELV+D +PL+YKPFDHF+FL Y
Sbjct: 241 WSNDRIRGCRHRVMMRGDETRYSLGLFSFKSGTISVPEELVEDNHPLKYKPFDHFEFLRY 300

Query: 542 DKANASHKTVSRIKDYCGL 556
           DKANASHKT SRIKDYCG+
Sbjct: 301 DKANASHKTCSRIKDYCGI 318

BLAST of Spg014229 vs. NCBI nr
Match: XP_038898684.1 (probable 2-oxoglutarate-dependent dioxygenase AOP1 [Benincasa hispida])

HSP 1 Score: 523.9 bits (1348), Expect = 1.7e-144
Identity = 247/318 (77.67%), Postives = 285/318 (89.62%), Query Frame = 0

Query: 241 IMGSQAKAKI-PTIDLSKEKLKAAGTEEWALSCKEMRVALEEFGCFLVVYDEVSLELHNS 300
           ++ S+AKAK+ PTIDLS  KL+A GTEEW L+CKEMR+ LEEFGCF VVYD VSLELHNS
Sbjct: 1   MVNSEAKAKVLPTIDLS--KLEAGGTEEWGLACKEMRLGLEEFGCFSVVYDRVSLELHNS 60

Query: 301 IFEATKELFDLPLESRIQNTSEKPYHGYFGRYSFLPLYESMAIDNPSDLQAAQAFANLMW 360
           IFEATKELF LPLE+RI+NTSEKPYHGYFG YS LPLYESM IDNP +LQA Q+FANLMW
Sbjct: 61  IFEATKELFHLPLETRIKNTSEKPYHGYFGGYSVLPLYESMGIDNPIELQATQSFANLMW 120

Query: 361 PA-GNNHFCETVQNFSTMVAKLDEMVTRMVFESYGVSKL-SDSNIESTNHLLRCFKYRVP 420
           P   NNHFC TV+ FST+VA+L++MVT+MV ESYG+SKL ++ N+ES N+LLRCFKYR P
Sbjct: 121 PTKENNHFCGTVERFSTLVAELEKMVTKMVLESYGLSKLVNEPNMESANYLLRCFKYRAP 180

Query: 421 EKDETDLGLHSHTDLTFFSIIHQHLIGGLQIQSLDDEWIEVEPSGHCSFIVMAGDAIMAW 480
           EKDETD+GLHSHTDLTFFSIIHQHLI GLQ+QSLDD+WI+++PSGHC FIVMAGDA+MAW
Sbjct: 181 EKDETDVGLHSHTDLTFFSIIHQHLISGLQVQSLDDQWIDIQPSGHCYFIVMAGDALMAW 240

Query: 481 SNERIRACRHRVIMRGNERRYSVGLFSFKSGTIGVPEELVDDANPLRYKPFDHFQFLSYD 540
           SNERIR+CRHRVIMRG E RYS+G+FSFK+GTI VPEELVDDANPLRYKPF+H+ FL+YD
Sbjct: 241 SNERIRSCRHRVIMRGEETRYSIGMFSFKNGTIAVPEELVDDANPLRYKPFNHYDFLTYD 300

Query: 541 KANASHKTVSRIKDYCGL 556
           KA ASH+ VSRIKDYCG+
Sbjct: 301 KAKASHQNVSRIKDYCGI 316

BLAST of Spg014229 vs. NCBI nr
Match: XP_008461057.1 (PREDICTED: probable 2-oxoglutarate-dependent dioxygenase AOP1 isoform X1 [Cucumis melo])

HSP 1 Score: 499.6 bits (1285), Expect = 3.5e-137
Identity = 239/319 (74.92%), Postives = 278/319 (87.15%), Query Frame = 0

Query: 240 VIMGSQAKAKIPTIDLSKEKLKAAGTEEWALSCKEMRVALEEFGCFLVVYDEVSLELHNS 299
           ++  +++K  IPTIDLSK ++   GT+EW L+CKEMR  LEEFGCF VVYD+VSLELHNS
Sbjct: 1   MVCEAKSKVVIPTIDLSKLEV---GTKEWGLACKEMRFGLEEFGCFSVVYDKVSLELHNS 60

Query: 300 IFEATKELFDLPLESRIQNTSEKPYHGYFGRYSFLPLYESMAIDNPSDLQAAQAFANLMW 359
           I EATKELF LP+E+R++NTSEKPYHGYFG YSFLPLYESMAIDNP  L++ Q+FANLMW
Sbjct: 61  IVEATKELFHLPIETRMKNTSEKPYHGYFGGYSFLPLYESMAIDNPIQLKSTQSFANLMW 120

Query: 360 PA-GNNHFCETVQNFSTMVAKLDEMVTRMVFESYGVSKLSDSNI-ESTNHLLRCFKYRVP 419
           P   NNHFC TV+ FST+VAKL++MVT+MV ESYGVSK+ +  I ESTN+LLR FKYRV 
Sbjct: 121 PTRDNNHFCVTVERFSTLVAKLEKMVTKMVLESYGVSKVVNEPIMESTNYLLRLFKYRVA 180

Query: 420 EKDETDLGLHSHTDLTFFSIIHQHLIGGLQIQSL-DDEWIEVEPSGHCSFIVMAGDAIMA 479
           EKDETD+GLHSHTDLTFFSIIHQHLI GLQIQSL DD+W++V PS HCSFIVMAGDA+MA
Sbjct: 181 EKDETDVGLHSHTDLTFFSIIHQHLISGLQIQSLNDDQWLDVHPSSHCSFIVMAGDALMA 240

Query: 480 WSNERIRACRHRVIMRGNERRYSVGLFSFKSGTIGVPEELVDDANPLRYKPFDHFQFLSY 539
           WSNERIR+CRHRVIMRG E RYS+G+FSFK+GTI VP+  VDDANPLRYKPF+H+ FL+Y
Sbjct: 241 WSNERIRSCRHRVIMRGEETRYSIGMFSFKNGTIEVPQGFVDDANPLRYKPFNHYDFLTY 300

Query: 540 DKANASHKTVSRIKDYCGL 556
           DKANASH T+SRIKDYCGL
Sbjct: 301 DKANASHNTISRIKDYCGL 316

BLAST of Spg014229 vs. ExPASy Swiss-Prot
Match: Q9ZTA3 (Probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Arabidopsis thaliana OX=3702 GN=AOP1 PE=2 SV=1)

HSP 1 Score: 233.8 bits (595), Expect = 4.7e-60
Identity = 124/302 (41.06%), Postives = 186/302 (61.59%), Query Frame = 0

Query: 249 KIPTIDLSKEKLKAAGTEEWALSCKEMRVALEEFGCFLVVYDEVSLELHNSIFEATKELF 308
           ++P ID S + LK  G+ +W     ++  ALE++GCF   +D++S+EL+ S+FEA ++LF
Sbjct: 13  QLPVIDFSDQNLK-PGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNRSVFEAMEDLF 72

Query: 309 DLPLESRIQNTSEKPYHGYFGRYSFLPLYESMAIDNPSDLQAAQAFANLMWP-AGNNHFC 368
           +LP+ ++ +N S KP+HGY        LYES+ I++ + L+    F   +WP  GN    
Sbjct: 73  ELPIPTKQRNVSSKPFHGYLCH----NLYESLGINDANVLEKVNDFTQQLWPDHGNKSIS 132

Query: 369 ETVQNFSTMVAKLDEMVTRMVFESYGVSKLSDSNIESTNHLLRCFKYRVPEKD------E 428
           ET+  FS  + +LD MV RM+ ES+G+    D ++ ST +L R  KY  P  D      E
Sbjct: 133 ETIHLFSEQLVELDLMVRRMIMESFGIENYIDEHLNSTYYLTRLMKYTSPPDDDDDDDEE 192

Query: 429 TDLGLHSHTDLTFFSIIHQHLIGGLQIQSLDDEWIEVEPSGHCSFIVMAGDAIMAWSNER 488
           T LGL SHTD    +I+HQ+ + GL++++ DD+WI+V+PS   S +VM GD++ A  N R
Sbjct: 193 TKLGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKPS-QDSVLVMVGDSLCALLNGR 252

Query: 489 IRACRHRVIMRGNERRYSVGLFSF-KSGT-IGVPEELVDDANPLRYKPFDHFQFLSYDKA 542
           + +  HRVIM G + RYS GLFS  K+G  I  PEELVD  +P  +KPF++  FL + + 
Sbjct: 253 LHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLHFFQT 308

BLAST of Spg014229 vs. ExPASy Swiss-Prot
Match: Q944Z5 (Probable 2-oxoglutarate-dependent dioxygenase AOP1 (Fragment) OS=Arabidopsis thaliana OX=3702 GN=AOP1 PE=2 SV=1)

HSP 1 Score: 232.6 bits (592), Expect = 1.0e-59
Identity = 124/300 (41.33%), Postives = 186/300 (62.00%), Query Frame = 0

Query: 249 KIPTIDLSKEKLKAAGTEEWALSCKEMRVALEEFGCFLVVYDEVSLELHNSIFEATKELF 308
           ++P ID S + LK  G+ +W     ++  ALE++GCF   +D++S+EL+ S+FEA ++LF
Sbjct: 6   QLPVIDFSDQNLK-PGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNRSVFEAMEDLF 65

Query: 309 DLPLESRIQNTSEKPYHGYFGRYSFLPLYESMAIDNPSDLQAAQAFANLMWP-AGNNHFC 368
           +LP+ ++ +N S K +HGY        LYES+ I++ + L+    F   +WP  GN    
Sbjct: 66  ELPIPTKQRNVSSKLFHGYLCH----NLYESLGINDANVLEKVNDFTQQLWPDHGNKSIS 125

Query: 369 ETVQNFSTMVAKLDEMVTRMVFESYGVSKLSDSNIESTNHLLRCFKYRVPEKD----ETD 428
           ET+  FS  + +LD MV RM+ ES+G+ K  D ++ ST +L R  KY  P  D    ET 
Sbjct: 126 ETIHLFSEQLVELDLMVRRMIMESFGIEKYIDEHLNSTYYLTRLMKYTSPPDDDDDEETK 185

Query: 429 LGLHSHTDLTFFSIIHQHLIGGLQIQSLDDEWIEVEPSGHCSFIVMAGDAIMAWSNERIR 488
           LGL SHTD    +I+HQ+ + GL++++ DD+WI+V+PS   S +VM GD++ A  N R+ 
Sbjct: 186 LGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKPS-QDSVLVMVGDSLCALLNGRLH 245

Query: 489 ACRHRVIMRGNERRYSVGLFSF-KSGT-IGVPEELVDDANPLRYKPFDHFQFLSYDKANA 542
           +  HRVIM G + RYS GLFS  K+G  I  PEELVD  +P  +KPF++  FL + +  A
Sbjct: 246 SPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLHFFQTEA 299

BLAST of Spg014229 vs. ExPASy Swiss-Prot
Match: A0A0N9HQ36 (Deoxypodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=2-ODD PE=1 SV=1)

HSP 1 Score: 231.1 bits (588), Expect = 3.0e-59
Identity = 124/311 (39.87%), Postives = 176/311 (56.59%), Query Frame = 0

Query: 242 MGSQAKAKIPTIDLSKEKLKAAGTEEWALSCKEMRVALEEFGCFLVVYDEVSLELHNSIF 301
           MGS A  ++P IDLS + LK  GT  W     ++R ALEE+GCF  V D VS EL  ++ 
Sbjct: 1   MGSTAPLRLPVIDLSMKNLK-PGTTSWNSVRTQVREALEEYGCFEAVIDAVSPELQKAVC 60

Query: 302 EATKELFDLPLESRIQNTSEKPYHGYFGRYSFLPLYESMAIDNPSDLQAAQAFANLMWPA 361
               EL +LPLE+++ N ++  Y G+    S   L E M +   +DL+  + F NLMWP 
Sbjct: 61  NKGHELLNLPLETKMLNGNKPEYDGF---TSIPNLNEGMGVGRITDLEKVERFTNLMWPE 120

Query: 362 GNNHFCETVQNFSTMVAKLDEMVTRMVFESYGVSKLSDSNIESTNHLLRCFKYRVPEKDE 421
           GN  FCETV ++   +A++D ++  MVFES+G+ K  DS  ESTN+LL   +Y+ P KD 
Sbjct: 121 GNKDFCETVYSYGKRMAEVDHILKMMVFESFGMEKHFDSFCESTNYLLHFMRYQQPGKDG 180

Query: 422 TDLGLHSHTDLTFFSIIHQHLIGGLQIQSLDDEWIEVEPSGHCSFIVMAGDAIMAWSNER 481
               L  H D +  +I++Q+ + GL+ ++ D EWI      H   IV+ GD  MAWSN R
Sbjct: 181 RSPALSLHKDKSILTIVNQNDVKGLEFETKDGEWILPTADNH---IVLLGDCFMAWSNGR 240

Query: 482 IRACRHRVIMRGNERRYSVGLFSFKSGTIGVPEELVDDANPLRYKPFDHFQFLSYDKANA 541
           + +  HRV +  N+ R S   FSF    I  P ELVD+ +PL + PF+  + L+Y     
Sbjct: 241 LHSPLHRVTLVANQARLSTSSFSFPKDIIETPAELVDEEHPLLFNPFEITELLAYCFTKE 300

Query: 542 SHKTVSRIKDY 553
             K V  +K Y
Sbjct: 301 GAKAVCDLKQY 304

BLAST of Spg014229 vs. ExPASy Swiss-Prot
Match: Q945B6 (Probable 2-oxoglutarate-dependent dioxygenase AOP1.2 OS=Arabidopsis thaliana OX=3702 GN=AOP1.2 PE=2 SV=1)

HSP 1 Score: 183.0 bits (463), Expect = 9.5e-45
Identity = 101/238 (42.44%), Postives = 146/238 (61.34%), Query Frame = 0

Query: 12  ELPVINFSGGDLKAEDGGEWRWTSKKVREALEEYGCFIAVYDRVSSELRGEIFKALKALF 71
           +LPVI+FS  +LK     +W      V +ALE+YGCF A +D++S EL   +F+A++ LF
Sbjct: 13  QLPVIDFSDQNLK-PGSSKWDEVKADVLKALEDYGCFEAFFDKLSVELNRSVFEAMEDLF 72

Query: 72  DLPVETKKKNKYEKPFNGYVGQLRTLPLHESLGIDDATNFHGVQSFANLMWPS-GNHHFS 131
           +LP+ TK++N   KPF+GY+       L+ESLGIDDA     V  F   +WP  GN   S
Sbjct: 73  ELPIPTKQRNVSSKPFHGYLCH----NLYESLGIDDANVLEKVNDFTQQLWPDHGNKSIS 132

Query: 132 ETLHSFAKIAEELDQMVTKMIFESYGVENYHHSHASSITYLLRVLKNKAP-----QSENP 191
           ET+H F++   ELD MV +MI ES+G+ENY   H +S  YL R++K  +P       E  
Sbjct: 133 ETIHLFSEQLVELDLMVRRMIMESFGIENYIDEHLNSTYYLTRLMKYTSPPDDDDDDEET 192

Query: 192 SLCLVDHTDKSFTTMVYQDHTNGLEVQTKDGQWIKVEISSPANFVVIAGDAFQLVIMG 244
            L L  HTDK+  T+++Q   +GLEV+TKD +WIKV+  S  + +V+ GD+   ++ G
Sbjct: 193 KLGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVK-PSQDSVLVMVGDSLCALLNG 244

BLAST of Spg014229 vs. ExPASy Swiss-Prot
Match: Q945B5 (2-oxoglutarate-dependent dioxygenase AOP2 OS=Arabidopsis thaliana OX=3702 GN=AOP2 PE=2 SV=1)

HSP 1 Score: 177.6 bits (449), Expect = 4.0e-43
Identity = 122/435 (28.05%), Postives = 193/435 (44.37%), Query Frame = 0

Query: 242 MGSQAKAKIPTIDLSKEKLKAAGTEEWALSCKEMRVALEEFGCFLVVYDEVSLELHNSIF 301
           MGS    ++P I+L+ + L+  G+ +WA    ++R ALE+FGCF   YD+VSLEL  SI 
Sbjct: 1   MGS-CSLQLPLINLADKTLE-PGSSKWAEVRSDVRKALEDFGCFEASYDKVSLELQESIM 60

Query: 302 EATKELFDLPLESRIQNTSEKPYHGYFGRYSFLPLYESMAIDNPSDLQAAQAFANLMWP- 361
           +  +ELF LP+E++ +N   KPY GY    +   L ES+ I N + L+    F   +WP 
Sbjct: 61  KTMEELFALPVETKQRNVCPKPYVGYLNHNN---LSESLGISNANILENVNEFTQQLWPD 120

Query: 362 -AGNNHFCETVQNFSTMVAKLDEMVTRMVFESYGVSKLSDSNIESTNHLLRCFKYRVP-- 421
             GN +  +T+Q F+  + ++D MV RMV ES+G+ K  D +++ST + +R  KY  P  
Sbjct: 121 GDGNENISKTIQLFAEKLVEIDVMVRRMVMESFGIEKYIDDHLKSTEYRMRLMKYIAPPE 180

Query: 422 ------------------------------------------------------------ 481
                                                                       
Sbjct: 181 GDANTTVDDYADLLAKLNIDGVEPNVGVKVNADISDDVNANPSVNAGVGANVNADTGVND 240

Query: 482 ------------------------------------------------------EKDETD 541
                                                                 + +E  
Sbjct: 241 NLNVDAEANGDANIAVGGGVNANTDLGVGVNVNSNVAVNAKTGATSGDDVEANDDNEEKK 300

Query: 542 LGLHSHTDLTFFSIIHQHLIGGLQIQSLDDEWIEVEPSGHCSFIVMAGDAIMAWSNERIR 556
           LGL  HTD   F+++ QH I GL++++ D++WI V+PS + +FIV+AGD++ A  N RIR
Sbjct: 301 LGLPCHTDKNLFTVLFQHEIEGLEVKTKDEKWIRVKPSPN-TFIVIAGDSLCALMNGRIR 360

BLAST of Spg014229 vs. ExPASy TrEMBL
Match: A0A498HFF2 (Uncharacterized protein OS=Malus domestica OX=3750 GN=DVH24_031377 PE=4 SV=1)

HSP 1 Score: 573.9 bits (1478), Expect = 7.1e-160
Identity = 299/632 (47.31%), Postives = 402/632 (63.61%), Query Frame = 0

Query: 1   MGSETPLMRTEELPVINFSGGDLKAEDGGEWRWTSKKVREALEEYGCFIAVYDRVSSELR 60
           MGSE      ++LPV++FS   LK      W    K V+ ALEE+GCF+ +YD++S E R
Sbjct: 20  MGSEV----EQKLPVLDFSKEALK-PGTNSWLLACKDVQRALEEFGCFVVLYDKISDEFR 79

Query: 61  GEIFKALKALFDLPVETKKKNKYEKPFNGYVGQLRTLPLHESLGIDDATNFHGVQSFANL 120
                +L+ LFDLP ETK  NKYEKP NGYVGQ+  LPLHESLGID ATNF   + F  L
Sbjct: 80  NAFVGSLEELFDLPTETKMTNKYEKPLNGYVGQIPKLPLHESLGIDCATNFEETKKFTKL 139

Query: 121 MWPSGNHHFSETLHSFAKIAEELDQMVTKMIFESYGVENYHHSHASSITYLLRVLKNKAP 180
           +         E  ++FAK+AEELDQMV +MIFESYGVE Y+ S+  SI+YLLR+L+N++P
Sbjct: 140 I---------ERAYAFAKVAEELDQMVARMIFESYGVEKYYDSYVGSISYLLRILQNRSP 199

Query: 181 QSENPSLCLVDHTDKSFTTMVYQDH-TNGLEVQTKDGQWIKVEISSPANFVVIAGDAFQL 240
           +    +L  V HTDKSFTT++YQ++  N LEV+T++ +WIKVE   P++F+V+AGDA   
Sbjct: 200 KENEHNLGFVAHTDKSFTTVLYQNNQVNALEVETRNHEWIKVEF-PPSSFIVMAGDALMA 259

Query: 241 ------------VI---------------------------------------------- 300
                       VI                                              
Sbjct: 260 WSNDRILSPNHRVIMSGNETRYSLAQFAFSDGEIHVPEELGDEERPLRYKSFDHPGLLRF 319

Query: 301 -------------------MGSQAKAKIPTIDLSKEKLKAAGTEEWALSCKEMRVALEEF 360
                              MGS+ + K+P IDLS E LK  GT+ W L+CKE++ ALEE+
Sbjct: 320 FRSQDRYISNSAIKAYCDRMGSETQPKLPVIDLSDENLK-PGTDAWLLACKEIKDALEEY 379

Query: 361 GCFLVVYDEVSLELHNSIFEATKELFDLPLESRIQNTSEKPYHGYFGRYSFLPLYESMAI 420
           GCF  +Y ++ LELHNSIF A ++LF LPLE++ Q TS++PYH YFG+YSFLPLYES+ +
Sbjct: 380 GCFEAIYHKIPLELHNSIFSAMEDLFGLPLETKKQKTSDRPYHSYFGQYSFLPLYESLGV 439

Query: 421 DNPSDLQAAQAFANLMWPAGNNHFCETVQNFSTMVAKLDEMVTRMVFESYGVSKLSDSNI 480
           DNP+ ++ AQ F +++         ET  +FS +VA+L +MVTRMVF+ YGV +L DS++
Sbjct: 440 DNPTTVEGAQGFTSII---------ETAHSFSKLVAELSQMVTRMVFDMYGVQRLFDSHM 499

Query: 481 ESTNHLLRCFKYRVPEKDETDLGLHSHTDLTFFSIIHQHLIGGLQIQSLDDEWIEVEPSG 540
           EST +LLRCFKYR  E++ET++GLH HTD TF SI++Q+ + GLQI + D +WI+V PS 
Sbjct: 500 ESTTYLLRCFKYRTQEENETNVGLHPHTDKTFISILNQNQVNGLQIGTKDGQWIDVNPS- 559

Query: 541 HCSFIVMAGDAIMAWSNERIRACRHRVIMRGNERRYSVGLFSFKSGTIGVPEELVDDANP 555
             SF+++AGDA MAWSN+R+ +C H+V M+ N+ RYS+GLF+FKSG I VPEEL+D+ +P
Sbjct: 560 PSSFLILAGDAFMAWSNDRVPSCDHQVTMKENKTRYSLGLFAFKSGIIQVPEELIDEKHP 619

BLAST of Spg014229 vs. ExPASy TrEMBL
Match: A0A6J1CXE5 (probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Momordica charantia OX=3673 GN=LOC111015077 PE=3 SV=1)

HSP 1 Score: 525.0 bits (1351), Expect = 3.8e-145
Identity = 250/319 (78.37%), Postives = 287/319 (89.97%), Query Frame = 0

Query: 242 MGSQAKAKIPTIDLSKEKLKAAGTEEWALSCKEMRVALEEFGCFLVVYDEVSLELHNSIF 301
           MG+QAK+KIPTI+LS+EKLK  G+EEW L  KE+RVALEEFGCFLVVY++VSLELHNSIF
Sbjct: 1   MGTQAKSKIPTIELSEEKLK-VGSEEWVLIGKEIRVALEEFGCFLVVYEKVSLELHNSIF 60

Query: 302 EATKELFDLPLESRIQNTSEKPYHGYFGRYSFLPLYESMAIDNPSDLQAAQAFANLMWPA 361
           EATKELF LP+E+RIQNTSEKPYHGYFGRYSFLPLYESMA+DNP+ LQA Q F NLMWPA
Sbjct: 61  EATKELFHLPIETRIQNTSEKPYHGYFGRYSFLPLYESMAVDNPTSLQAIQTFTNLMWPA 120

Query: 362 GNNHFCETVQNFSTMVAKLDEMVTRMVFESYGVSK-LSDSNIESTNHLLRCFKYRVPEKD 421
           GN  FCETV +FST+VAKL+E VTRMV ESYGVSK + +S+IEST HLLRCFKYRVP++D
Sbjct: 121 GNKTFCETVHDFSTLVAKLEETVTRMVLESYGVSKQVCESSIESTTHLLRCFKYRVPKQD 180

Query: 422 ETDLGLHSHTDLTFFSIIHQHLIGGLQIQSLDD----EWIEVEPSGHCSFIVMAGDAIMA 481
           ETDLGLHSHTDLTF SI+HQHLIGGLQ++SLD+    EWI+++P G  SFIVMAGDA+MA
Sbjct: 181 ETDLGLHSHTDLTFLSILHQHLIGGLQVKSLDNNNDGEWIDIDPVGRSSFIVMAGDALMA 240

Query: 482 WSNERIRACRHRVIMRGNERRYSVGLFSFKSGTIGVPEELVDDANPLRYKPFDHFQFLSY 541
           WSN+RIR CRHRV+MRG+E RYS+GLFSFKSGTI VPEELV+D +PL+YKPFDHF+FL Y
Sbjct: 241 WSNDRIRGCRHRVMMRGDETRYSLGLFSFKSGTISVPEELVEDNHPLKYKPFDHFEFLRY 300

Query: 542 DKANASHKTVSRIKDYCGL 556
           DKANASHKT SRIKDYCG+
Sbjct: 301 DKANASHKTCSRIKDYCGI 318

BLAST of Spg014229 vs. ExPASy TrEMBL
Match: A0A1S3CDV2 (probable 2-oxoglutarate-dependent dioxygenase AOP1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103499759 PE=3 SV=1)

HSP 1 Score: 499.6 bits (1285), Expect = 1.7e-137
Identity = 239/319 (74.92%), Postives = 278/319 (87.15%), Query Frame = 0

Query: 240 VIMGSQAKAKIPTIDLSKEKLKAAGTEEWALSCKEMRVALEEFGCFLVVYDEVSLELHNS 299
           ++  +++K  IPTIDLSK ++   GT+EW L+CKEMR  LEEFGCF VVYD+VSLELHNS
Sbjct: 1   MVCEAKSKVVIPTIDLSKLEV---GTKEWGLACKEMRFGLEEFGCFSVVYDKVSLELHNS 60

Query: 300 IFEATKELFDLPLESRIQNTSEKPYHGYFGRYSFLPLYESMAIDNPSDLQAAQAFANLMW 359
           I EATKELF LP+E+R++NTSEKPYHGYFG YSFLPLYESMAIDNP  L++ Q+FANLMW
Sbjct: 61  IVEATKELFHLPIETRMKNTSEKPYHGYFGGYSFLPLYESMAIDNPIQLKSTQSFANLMW 120

Query: 360 PA-GNNHFCETVQNFSTMVAKLDEMVTRMVFESYGVSKLSDSNI-ESTNHLLRCFKYRVP 419
           P   NNHFC TV+ FST+VAKL++MVT+MV ESYGVSK+ +  I ESTN+LLR FKYRV 
Sbjct: 121 PTRDNNHFCVTVERFSTLVAKLEKMVTKMVLESYGVSKVVNEPIMESTNYLLRLFKYRVA 180

Query: 420 EKDETDLGLHSHTDLTFFSIIHQHLIGGLQIQSL-DDEWIEVEPSGHCSFIVMAGDAIMA 479
           EKDETD+GLHSHTDLTFFSIIHQHLI GLQIQSL DD+W++V PS HCSFIVMAGDA+MA
Sbjct: 181 EKDETDVGLHSHTDLTFFSIIHQHLISGLQIQSLNDDQWLDVHPSSHCSFIVMAGDALMA 240

Query: 480 WSNERIRACRHRVIMRGNERRYSVGLFSFKSGTIGVPEELVDDANPLRYKPFDHFQFLSY 539
           WSNERIR+CRHRVIMRG E RYS+G+FSFK+GTI VP+  VDDANPLRYKPF+H+ FL+Y
Sbjct: 241 WSNERIRSCRHRVIMRGEETRYSIGMFSFKNGTIEVPQGFVDDANPLRYKPFNHYDFLTY 300

Query: 540 DKANASHKTVSRIKDYCGL 556
           DKANASH T+SRIKDYCGL
Sbjct: 301 DKANASHNTISRIKDYCGL 316

BLAST of Spg014229 vs. ExPASy TrEMBL
Match: A0A6J1JXN6 (probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita maxima OX=3661 GN=LOC111488804 PE=3 SV=1)

HSP 1 Score: 497.7 bits (1280), Expect = 6.4e-137
Identity = 232/316 (73.42%), Postives = 274/316 (86.71%), Query Frame = 0

Query: 242 MGSQAKAKIPTIDLSKEKLKAAGTEEWALSCKEMRVALEEFGCFLVVYDEVSLELHNSIF 301
           MGS+ +A++P IDLSK +   AGTE W  +CK MRV LEEFGCF VV++EVSLELHNS F
Sbjct: 1   MGSETQARVPVIDLSKLETLEAGTEAWGSTCKAMRVGLEEFGCFCVVFEEVSLELHNSFF 60

Query: 302 EATKELFDLPLESRIQNTSEKPYHGYFGRYSFLPLYESMAIDNPSDLQAAQAFANLMWPA 361
            A  ELF LP+E+RI+NTSEK YHGYFGRYSFLPLYESMAI+NP+ L AAQ+FANLMWPA
Sbjct: 61  GAVDELFQLPIETRIKNTSEKAYHGYFGRYSFLPLYESMAIENPTQLDAAQSFANLMWPA 120

Query: 362 -GNNHFCETVQNFSTMVAKLDEMVTRMVFESYGVSK-LSDSNIESTNHLLRCFKYRVPEK 421
            GNNHF ET+Q FST++AKLD+++TRMV ESYGV+K L D+NI+ST +LLR FKYRVPE+
Sbjct: 121 QGNNHFSETIQRFSTLMAKLDKIITRMVLESYGVTKQLIDTNIDSTANLLRFFKYRVPEE 180

Query: 422 DETDLGLHSHTDLTFFSIIHQHLIGGLQIQSLDDEWIEVEPSGHCSFIVMAGDAIMAWSN 481
            ETD+GLH+HTDLTFFSIIHQHLIGGLQIQSLDD+WI++EPSGHCSF+VMAGDA+MAWSN
Sbjct: 181 HETDVGLHAHTDLTFFSIIHQHLIGGLQIQSLDDQWIDLEPSGHCSFVVMAGDALMAWSN 240

Query: 482 ERIRACRHRVIMRGNERRYSVGLFSFKSGTIGVPEELVDDANPLRYKPFDHFQFLSYDKA 541
           +RIR+CRHRV M+  E RYS G+FSFK+GTI VPEELVDDANPLRYKPF+HF FL+YDKA
Sbjct: 241 DRIRSCRHRVTMKAEETRYSAGMFSFKNGTISVPEELVDDANPLRYKPFNHFDFLTYDKA 300

Query: 542 NASHKTVSRIKDYCGL 556
             S K + RI+DYC +
Sbjct: 301 KGSKKFLYRIRDYCSI 316

BLAST of Spg014229 vs. ExPASy TrEMBL
Match: A0A4D6MWC0 (Gibberellin 20-oxidase OS=Vigna unguiculata OX=3917 GN=DEO72_LG9g401 PE=4 SV=1)

HSP 1 Score: 496.9 bits (1278), Expect = 1.1e-136
Identity = 257/621 (41.38%), Postives = 373/621 (60.06%), Query Frame = 0

Query: 1   MGSETPLMRTEELPVINFSGGDLKAEDGGEWRWTSKKVREALEEYGCFIAVYDRVSSELR 60
           MGSE    +   +PV++FS  D K      W    + VR+ALEE+GCF+AVYD+   EL 
Sbjct: 1   MGSEE---QHPMIPVLDFSMEDSK-PGSDSWLERCRSVRQALEEHGCFVAVYDKACEELE 60

Query: 61  GEIFKALKALFDLPVETKKKNKYE-KPFNGYVGQLRTLPLHESLGIDDATNFHGVQSFAN 120
             +F ++K LFDL  ETK++N YE  P  GYVGQL  +PLH+S+GID+ T   G+QSFA+
Sbjct: 61  NGVFCSMKELFDLSHETKQRNTYEGSPLKGYVGQLPQIPLHQSMGIDEGTTLEGIQSFAH 120

Query: 121 LMWPSGNHHFSETLHSFAKIAEELDQMVTKMIFESYGVENYHHSHASSITYLLRVLKNKA 180
            MWP GNH F + +  +AK+AEELD+MV +MIFESYG+  Y+ S+  S +YLLR+L + A
Sbjct: 121 KMWPKGNHQFCKYIFEYAKVAEELDRMVARMIFESYGLSEYYDSYIGSTSYLLRLLAHTA 180

Query: 181 PQSENPSLCLVDHTDKSFTTMVYQDHTNGLEVQTKDGQWIKVEISSPANFVVIAGDAF-- 240
           P+   P L LV HTDKSFTT+++Q+H N L V+T++G WI V+ SS ++FVV+AGD    
Sbjct: 181 PEKVEPQLGLVAHTDKSFTTILHQNHVNALMVETRNGAWIHVDFSSTSSFVVMAGDGLMA 240

Query: 241 --------------------------------------QLV------------------- 300
                                                 +L+                   
Sbjct: 241 WSNDRIQSPNHKVVMNGNETRYSLGLFAFSKGILKVPEELIDEEHPVQYKPFHHLGLLNF 300

Query: 301 -----IMGSQAKAKIPTIDLSKEKLKAAGTEEWALSCKEMRVALEEFGCFLVVYDEVSLE 360
                +MGS+ + ++P +D + + LK  GT  W  +C+ +R ALE+ G FL +YD ++ E
Sbjct: 301 TYIQRVMGSETQCELPLVDFTNQNLK-PGTHPWLSTCEVVRSALEDHGAFLALYDNITFE 360

Query: 361 LHNSIFEATKELFDLPLESRIQNTSEKPYHGYFGRYSFLPLYESMAIDNPSDLQAAQAFA 420
            ++S++   K  FDL  E++ + T+EKP   Y G+   +PLYES  I NP   Q  Q F 
Sbjct: 361 TYDSVYSEMKNFFDLSTETKRRKTTEKPIFSYSGQRPQIPLYESAGIVNPLSFQDCQKFT 420

Query: 421 NLMWPAGNNHFCETVQNFSTMVAKLDEMVTRMVFESYGVS-KLSDSNIESTNHLLRCFKY 480
           ++MWP GN++FCE+V +++  V KLD +V RM+FESYGV  K  +  +EST ++LR +KY
Sbjct: 421 HVMWPQGNHNFCESVHSYAKQVVKLDHIVKRMMFESYGVERKKLECLLESTEYVLRGYKY 480

Query: 481 RVPEKDETDLGLHSHTDLTFFSIIHQHLIGGLQIQSLDDEWIEVEPSGHCSFIVMAGDAI 540
           R+PE+ E++LG+  H+D    +I++Q    GL ++  + EW EV  S    F+VM GDA+
Sbjct: 481 RIPEEGESNLGVAPHSDTALLTILNQK-AEGLGVKLKNGEWFEVGVSPSL-FLVMGGDAL 540

Query: 541 MAWSNERIRACRHRVIMRGNERRYSVGLFSFKSGTIGVPEELVDDANPLRYKPFDHFQFL 556
           M WSN RI AC HRVI++  + RYS+GL S+    +   E+LVD+ +PLRYKP DH+ +L
Sbjct: 541 MVWSNGRIGACEHRVIVKSKKERYSMGLLSYAGKIMEPEEKLVDEEHPLRYKPLDHYGYL 600

BLAST of Spg014229 vs. TAIR 10
Match: AT1G52800.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 306.2 bits (783), Expect = 5.3e-83
Identity = 140/307 (45.60%), Postives = 203/307 (66.12%), Query Frame = 0

Query: 249 KIPTIDLSKEKLKAAGTEEWALSCKEMRVALEEFGCFLVVYDEVSLELHNSIFEATKELF 308
           K+P +DL+ ++     T  W    +E   ALEE+GCFL VYD V+ +L +SIF A +ELF
Sbjct: 9   KVPILDLTSQQELKPNTSTWRSVSREACEALEEYGCFLAVYDGVTQQLDDSIFAAAEELF 68

Query: 309 DLPLESRIQNTSEKPYHGYFGRYSFLPLYESMAIDNPSDLQAAQAFANLMWPAGNNHFCE 368
           DLP E++ +N +EKPYHGY G+   +PL+E + +D  ++ + AQ F +LMWP GN+ FC 
Sbjct: 69  DLPTETKKKNVNEKPYHGYVGQMPVIPLHEGLGVDYVTNKEIAQRFTHLMWPQGNDRFCN 128

Query: 369 TVQNFSTMVAKLDEMVTRMVFESYGVSKLSDSNIESTNHLLRCFKYRVPEKDETDLGLHS 428
           TV  FS  VA+LD +V RM+FE+YGV K  +S++ S  +LL+  KY  P +  +      
Sbjct: 129 TVHTFSNAVAELDRLVVRMIFENYGVEKHYESHVGSKTYLLKFLKYLAPPESISMPAFPQ 188

Query: 429 HTDLTFFSIIHQHLIGGLQIQSLDDEWIEVEPSGHCSFIVMAGDAIMAWSNERIRACRHR 488
           HTD TF SI+HQ+ + GL+++S D EWI ++     S++VMAGD  M WSN+RIR+C HR
Sbjct: 189 HTDKTFLSILHQNDVNGLEVKSKDGEWISLQLPPK-SYVVMAGDISMGWSNDRIRSCEHR 248

Query: 489 VIMRGNERRYSVGLFSFKSGTIGVPEELVDDANPLRYKPFDHFQFLSYDKANASHKTVSR 548
           V M G++ RY++GLFSF +  + +PEELVDD +PL YKPFD+   +++       +  S 
Sbjct: 249 VTMEGDKTRYTLGLFSFLTDLVSIPEELVDDKHPLMYKPFDNIALINFYTTKEGREANST 308

Query: 549 IKDYCGL 556
           +K YCG+
Sbjct: 309 LKAYCGI 314

BLAST of Spg014229 vs. TAIR 10
Match: AT1G52790.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 298.5 bits (763), Expect = 1.1e-80
Identity = 139/307 (45.28%), Postives = 209/307 (68.08%), Query Frame = 0

Query: 249 KIPTIDLSKEKLKAAGTEEWALSCKEMRVALEEFGCFLV-VYDEVSLELHNSIFEATKEL 308
           KIPT+D S+E LK  GT+ W  + + +R ALEE+GCF++ + D+  L+L + +F +  +L
Sbjct: 4   KIPTLDFSREDLK-PGTKYWESTRENIRQALEEYGCFIIDLKDKTPLDLLDRVFGSLVDL 63

Query: 309 FDLPLESRIQNTSEKPYHGYFGRYSFLPLYESMAIDNPSDLQAAQAFANLMWPAGNNHFC 368
           FDLP +++++N  +KP +GY G+   LPL+ES+ IDN + L+A ++F  LMWP GN HF 
Sbjct: 64  FDLPTQTKMKNKYDKPLNGYVGQIPALPLHESLGIDNATSLEATRSFTGLMWPQGNEHFS 123

Query: 369 ETVQNFSTMVAKLDEMVTRMVFESYGVSKLSDSNIESTNHLLRCFKYRVPEKDETDLGLH 428
           E +  ++   A+LD+MVTRMVF+SY V K  D  IEST +LLR  K R P  +   LG  
Sbjct: 124 ECLYKYAEFAAELDQMVTRMVFQSYNVEKYYDPYIESTTYLLRVLKNRAPNNENPTLGFV 183

Query: 429 SHTDLTFFSIIHQHLIGGLQIQSLDDEWIEVEPSGHCSFIVMAGDAIMAWSNERIRACRH 488
           +HTD +F +I+HQ  + GL++++ + E I +  S    F+V+AGDA+MAWSN+R+ + RH
Sbjct: 184 THTDKSFTTILHQDQVNGLEMETREGERININLSSPSLFMVVAGDALMAWSNDRVWSPRH 243

Query: 489 RVIMRGNERRYSVGLFSFKSGTIGVPEELVDDANPLRYKPFDHFQFLSYDKANASHKTVS 548
           +V++ G   RYS+G+F+F +GT+ VPEEL+D  +PL YKPFDH   L + + +  +K+  
Sbjct: 244 QVLVSGETDRYSLGMFAFNNGTLQVPEELIDHQHPLMYKPFDHLGLLRFYRTDTGYKSEC 303

Query: 549 RIKDYCG 555
            IK YCG
Sbjct: 304 PIKAYCG 309

BLAST of Spg014229 vs. TAIR 10
Match: AT1G52820.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 283.5 bits (724), Expect = 3.7e-76
Identity = 136/319 (42.63%), Postives = 210/319 (65.83%), Query Frame = 0

Query: 242 MGSQA---KAKIPTIDLSKEKLKAAGTEEWALSCKEMRVALEEFGCFLVVYDEVSLELHN 301
           MGS+      ++P ID S + LK  G  EW L+  +++ AL+++G F   +D +  EL  
Sbjct: 1   MGSETPLLPLRLPVIDFSNKNLK-PGEPEWDLTRADVQKALQDYGYFEASFDRIPFELRK 60

Query: 302 SIFEATKELFDLPLESRIQNTSEKPYHGYFGRYSFLPLYESMAIDNPSDLQAAQAFANLM 361
           S+F A +ELFDLPL+++++N S+KP+HGY G+Y  +PLYESM ID+    +   AF   +
Sbjct: 61  SVFGALEELFDLPLQTKLRNVSKKPFHGYVGQYPMVPLYESMGIDDSDIAEKVDAFTEKL 120

Query: 362 WPAGNNHFCETVQNFSTMVAKLDEMVTRMVFESYGVSKLSDSNIESTNHLLRCFKYRVPE 421
           WP GN  F  T+Q+FS  +++LD  + RM+ ES+G+ K  D ++ STN+LLR  KY+ P+
Sbjct: 121 WPQGNISFSTTIQSFSKKLSELDITIRRMIMESFGLDKYIDEHLHSTNYLLRVMKYKGPD 180

Query: 422 KDETDLGLHSHTDLTFFSIIHQHLIGGLQIQSLDDEWIEVEPSGHCSFIVMAGDAIMAWS 481
            +ET +GL++HTD    +I++Q+ + GL++Q+ D  WI+V+P+   SF VM GD++ A  
Sbjct: 181 TEETKVGLNAHTDKNIVTILYQNHVEGLEVQTKDKNWIKVKPT-QDSFTVMIGDSLYALL 240

Query: 482 NERIRACRHRVIMRGNERRYSVGLFSF-KSG-TIGVPEELVDDANPLRYKPFDHFQFLSY 541
           N R+ +  HRV+M G E RYS+GLFS  K+G  +  P+ELVD+ +P  +KPFDH +FL +
Sbjct: 241 NGRLHSPYHRVMMTGTETRYSLGLFSIPKAGHIVSSPDELVDEEHPRLFKPFDHVEFLQF 300

Query: 542 DKANASHKTVSRIKDYCGL 556
               A  ++ S +K YCG+
Sbjct: 301 YYTEAGQRSQSALKTYCGI 317

BLAST of Spg014229 vs. TAIR 10
Match: AT1G15540.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 256.5 bits (654), Expect = 4.8e-68
Identity = 139/321 (43.30%), Postives = 190/321 (59.19%), Query Frame = 0

Query: 242 MGSQAKAKIPTIDLSKEKLKAAGTEEWALSCKEMRVALEEFGCFLVVYDEVSLELHNSIF 301
           MG     ++P I+LS + LK   TE W+ +   +R A+E  G F+  Y+     LH SI 
Sbjct: 1   MGGNETRELPIINLSDKNLK-QNTELWSFTRDSVREAMEHHGWFVAEYNNFPTGLHQSIL 60

Query: 302 EATKELFDLPLESRIQNTSEKPYHGYFGRYS-FLPLYESMAIDNPSDLQAAQAFANLMWP 361
           EA KEL DLP+E +++N + K  HGY    S   P++E + ID  +D+Q  + F+ LMWP
Sbjct: 61  EAAKELLDLPVEIKLKNENHKAGHGYITMMSDGQPVHEGLGIDQVNDVQQCRGFSRLMWP 120

Query: 362 ---AGNNHFCETVQNFSTMVAKLDEMVTRMVFESYGVSKLSDSNIESTNHLLRCFKYRVP 421
                N+ FCETV  ++ M A+L+++V RM+FESY V K ++  I  T +LLR  KYR  
Sbjct: 121 DDHDDNDRFCETVHAYAKMQAELEQLVIRMLFESYNVEKYTEKYIGGTRYLLRLLKYRRL 180

Query: 422 EKDETDLGLHSHTDLTFFSIIHQHLIGGLQIQS-LDDEWIEVEPSGHCSFIVMAGDAIMA 481
              E +    SHTD +F SI+HQ+ I GL ++S  +D W    PS    F+V+AGDAIMA
Sbjct: 181 PNGEPNRKFISHTDKSFISILHQNHITGLMLKSEKEDVWYHFTPS-PTRFVVIAGDAIMA 240

Query: 482 WSNERIRACRHRVIMRGNERRYSVGLFSFKSGTIGVPEELVDDANPLRYKPFDHFQFLSY 541
           WSN+RI+AC H+V M   E RYS+G FSF+ G I  PEE+VD  +PL Y PF H   L Y
Sbjct: 241 WSNDRIKACYHKVEMESVEMRYSLGFFSFQEGMISTPEEMVDKDHPLAYNPFHHDGLLEY 300

Query: 542 DKANASHKTVSR--IKDYCGL 556
            +    H    R   K YCG+
Sbjct: 301 YETLEVHLKAHRTMTKAYCGI 319

BLAST of Spg014229 vs. TAIR 10
Match: AT1G80320.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 251.1 bits (640), Expect = 2.0e-66
Identity = 126/320 (39.38%), Postives = 198/320 (61.88%), Query Frame = 0

Query: 242 MGSQAKAKIPTIDLSKEKLKAAGTEEWALSCKEMRVALEEFGCFLVVYDEVSLELHNSIF 301
           M S  +  +P +D S +KL   GT  W  +  ++R A+E  G F+  +  VS +L +++ 
Sbjct: 1   MDSHEEQCLPILDFSSDKL-VRGTSHWITTRDDVRRAMEGQGWFVAEFSGVSSDLRDNLL 60

Query: 302 EATKELFDLPLESRIQNTSEKPYHGYFGR-YSFLPLYESMAIDNPSDLQAAQAFANLMWP 361
              KE++ LP + +I+N + K  HGY         ++ES+ ID  ++LQA + F+ L+WP
Sbjct: 61  AGMKEMYYLPDQIKIKNENHKASHGYMSMVVDDYRIHESLGIDYATELQACKDFSKLLWP 120

Query: 362 AGNNHFCETVQNFSTMVAKLDEMVTRMVFESYGV--SKLSDSNIESTNHLLRCFKYRVPE 421
            GN+ FC+T   ++  +A+LD+ V RM++ESYG+   K S S+ EST +LLR   YR  +
Sbjct: 121 QGNDPFCQTTHMYAMTMAELDQTVMRMLYESYGMDEKKHSVSHSESTRYLLRMLSYRRQQ 180

Query: 422 KDETDLGLHSHTDLTFFSIIHQHLIGGLQIQSLDDEWIEVEPSGHCSFIVMAGDAIMAWS 481
             E + G  SHTD +F SI+HQ+ +GGLQ++++  +W+   PS    F+V++G  + AWS
Sbjct: 181 NGEANTGFVSHTDKSFMSILHQNHVGGLQLKTMTGQWVGFNPS-PTRFVVLSGMGLTAWS 240

Query: 482 NERIRACRHRVIMRGNERRYSVGLFSFKSGTIGVPEELVDDANPLRYKPFDHFQFLSYDK 541
           N+RI+AC H+V+M  +E RYS+G FSF  GTI  PEELVDD +PLRY PF+H   L + +
Sbjct: 241 NDRIKACYHKVVMSADEIRYSLGFFSFHKGTIRTPEELVDDQHPLRYNPFEHDGLLRFYE 300

Query: 542 A---NASHKTVSRIKDYCGL 556
           +   +    +   ++ YCG+
Sbjct: 301 SYINSLKKSSEDLLQIYCGV 318