Spg014158 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg014158
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Descriptionbasic 7S globulin 2-like
Locationscaffold3: 43465872 .. 43467224 (-)
RNA-Seq ExpressionSpg014158
SyntenySpg014158
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGCACTCGAGCTTCTATCTTGTTCTTCATTTTCTTTCTCCTTTCGTTTCCTTTCCTTTCTCTCCAAACAGCCTTCGTCGCTCCCATTCTCAAAGACCACAACTCTCTTCTCTACTCCATCTCCGTCCACCTCAAAACTCCGCTCCGGCCGGCCAACCTCCACCTCGACCTCGGCGGCGCCTTCTCCTGGATCGACTGCTACAACCGTTACAATTCTTCCTCTTACCGCCTCGTCAAATGCGATTCCGCTCTCTGCAACTCCATGGACAGTAAAGTTTGTGGCTCCTGTGTCGAAGCTCCGGCTCCAATCTGCGCTAACGACACCATCTTTTGCGACGTCTACCCCGAGAAACCGTCGATTCGAGATCAATACGTCGATTACAATCACCCGAAGCTCACGGATTCCGAGAATTTGATCACCGATGTTCTTGCTCTCTCCACCACTGACGGCTCCAGTTCAGGTCCACTCTGTCCGATTTCGGACATTCCGTTTTCCTGCGCCAAGACCGATTTCCTCCGAGGACTTGCCAGAAATGTGATCGGCCTGGCGGCGCTCGGCCGTTCGAACGAGTCGATTCCGTCGATGATCAGCGCGAAGTTCAATAGCCCTAGGATCTTCGCGGTTTGCTTATCGGGAACGAGATCGGGATCTGGTGTGGCTTTCTTTGGATCGAGAGGACCGTACGGATTTTTCCCCAATGTCGACCTTTCGAAATCCCTAATTTACACTCCGCTGCTCTTCAATCCGGTGAGTGGCTCGATTTACACTTGGTGGTTACCGTCTTATGAATATTACATTGGACTGTCCGCCATCAGAATCAACGGCAAGGCGGTGCCGTTCAATACTTCTCTGCTGGCGTTTGAGCCGGTTCACGGCGGCGGCGGGACGAAGATCAGCACCTCCACGACTTACGCGCTGCTGCAGACCTCCATTTACAGAGGTTTCGCGACGGCATTCATGAAGGAAGCGGCTGCGCTGAACTTCACGCTGACGAATGCGGTAGAGCCGTTCGGCGTGTGCTTTGCGGCGGACAGCGTTGCAGTGACGGCGGCGGGACCAATGGCGCCGGCGGTGGATTTGGTGATGGAGAACGGGAAAGTGGTGTGGAAATTGGGGGGCAGGAATTCGATGGTGAGGATTAAGAAGAACGGAGTGGATGCGTGGTGCTTGGGATTCATCAATGGCGGAGAATTTCCGAGGACGCCGATCGTGATCGGAGGTCTGCAAATGGAGGATCATTTGCTGCAGTTTGATCTCGAAAATTACAGATTTGGATTCAGTTCTTCGGCGTTGCTAAAGGGAACTTCGTGTTCCAACTTTAACTTCACTTCTGCTCAGAGTTTCCTATAA

mRNA sequence

ATGGGCACTCGAGCTTCTATCTTGTTCTTCATTTTCTTTCTCCTTTCGTTTCCTTTCCTTTCTCTCCAAACAGCCTTCGTCGCTCCCATTCTCAAAGACCACAACTCTCTTCTCTACTCCATCTCCGTCCACCTCAAAACTCCGCTCCGGCCGGCCAACCTCCACCTCGACCTCGGCGGCGCCTTCTCCTGGATCGACTGCTACAACCGTTACAATTCTTCCTCTTACCGCCTCGTCAAATGCGATTCCGCTCTCTGCAACTCCATGGACAGTAAAGTTTGTGGCTCCTGTGTCGAAGCTCCGGCTCCAATCTGCGCTAACGACACCATCTTTTGCGACGTCTACCCCGAGAAACCGTCGATTCGAGATCAATACGTCGATTACAATCACCCGAAGCTCACGGATTCCGAGAATTTGATCACCGATGTTCTTGCTCTCTCCACCACTGACGGCTCCAGTTCAGGTCCACTCTGTCCGATTTCGGACATTCCGTTTTCCTGCGCCAAGACCGATTTCCTCCGAGGACTTGCCAGAAATGTGATCGGCCTGGCGGCGCTCGGCCGTTCGAACGAGTCGATTCCGTCGATGATCAGCGCGAAGTTCAATAGCCCTAGGATCTTCGCGGTTTGCTTATCGGGAACGAGATCGGGATCTGGTGTGGCTTTCTTTGGATCGAGAGGACCGTACGGATTTTTCCCCAATGTCGACCTTTCGAAATCCCTAATTTACACTCCGCTGCTCTTCAATCCGGTGAGTGGCTCGATTTACACTTGGTGGTTACCGTCTTATGAATATTACATTGGACTGTCCGCCATCAGAATCAACGGCAAGGCGGTGCCGTTCAATACTTCTCTGCTGGCGTTTGAGCCGGTTCACGGCGGCGGCGGGACGAAGATCAGCACCTCCACGACTTACGCGCTGCTGCAGACCTCCATTTACAGAGGTTTCGCGACGGCATTCATGAAGGAAGCGGCTGCGCTGAACTTCACGCTGACGAATGCGGTAGAGCCGTTCGGCGTGTGCTTTGCGGCGGACAGCGTTGCAGTGACGGCGGCGGGACCAATGGCGCCGGCGGTGGATTTGGTGATGGAGAACGGGAAAGTGGTGTGGAAATTGGGGGGCAGGAATTCGATGGTGAGGATTAAGAAGAACGGAGTGGATGCGTGGTGCTTGGGATTCATCAATGGCGGAGAATTTCCGAGGACGCCGATCGTGATCGGAGGTCTGCAAATGGAGGATCATTTGCTGCAGTTTGATCTCGAAAATTACAGATTTGGATTCAGTTCTTCGGCGTTGCTAAAGGGAACTTCGTGTTCCAACTTTAACTTCACTTCTGCTCAGAGTTTCCTATAA

Coding sequence (CDS)

ATGGGCACTCGAGCTTCTATCTTGTTCTTCATTTTCTTTCTCCTTTCGTTTCCTTTCCTTTCTCTCCAAACAGCCTTCGTCGCTCCCATTCTCAAAGACCACAACTCTCTTCTCTACTCCATCTCCGTCCACCTCAAAACTCCGCTCCGGCCGGCCAACCTCCACCTCGACCTCGGCGGCGCCTTCTCCTGGATCGACTGCTACAACCGTTACAATTCTTCCTCTTACCGCCTCGTCAAATGCGATTCCGCTCTCTGCAACTCCATGGACAGTAAAGTTTGTGGCTCCTGTGTCGAAGCTCCGGCTCCAATCTGCGCTAACGACACCATCTTTTGCGACGTCTACCCCGAGAAACCGTCGATTCGAGATCAATACGTCGATTACAATCACCCGAAGCTCACGGATTCCGAGAATTTGATCACCGATGTTCTTGCTCTCTCCACCACTGACGGCTCCAGTTCAGGTCCACTCTGTCCGATTTCGGACATTCCGTTTTCCTGCGCCAAGACCGATTTCCTCCGAGGACTTGCCAGAAATGTGATCGGCCTGGCGGCGCTCGGCCGTTCGAACGAGTCGATTCCGTCGATGATCAGCGCGAAGTTCAATAGCCCTAGGATCTTCGCGGTTTGCTTATCGGGAACGAGATCGGGATCTGGTGTGGCTTTCTTTGGATCGAGAGGACCGTACGGATTTTTCCCCAATGTCGACCTTTCGAAATCCCTAATTTACACTCCGCTGCTCTTCAATCCGGTGAGTGGCTCGATTTACACTTGGTGGTTACCGTCTTATGAATATTACATTGGACTGTCCGCCATCAGAATCAACGGCAAGGCGGTGCCGTTCAATACTTCTCTGCTGGCGTTTGAGCCGGTTCACGGCGGCGGCGGGACGAAGATCAGCACCTCCACGACTTACGCGCTGCTGCAGACCTCCATTTACAGAGGTTTCGCGACGGCATTCATGAAGGAAGCGGCTGCGCTGAACTTCACGCTGACGAATGCGGTAGAGCCGTTCGGCGTGTGCTTTGCGGCGGACAGCGTTGCAGTGACGGCGGCGGGACCAATGGCGCCGGCGGTGGATTTGGTGATGGAGAACGGGAAAGTGGTGTGGAAATTGGGGGGCAGGAATTCGATGGTGAGGATTAAGAAGAACGGAGTGGATGCGTGGTGCTTGGGATTCATCAATGGCGGAGAATTTCCGAGGACGCCGATCGTGATCGGAGGTCTGCAAATGGAGGATCATTTGCTGCAGTTTGATCTCGAAAATTACAGATTTGGATTCAGTTCTTCGGCGTTGCTAAAGGGAACTTCGTGTTCCAACTTTAACTTCACTTCTGCTCAGAGTTTCCTATAA

Protein sequence

MGTRASILFFIFFLLSFPFLSLQTAFVAPILKDHNSLLYSISVHLKTPLRPANLHLDLGGAFSWIDCYNRYNSSSYRLVKCDSALCNSMDSKVCGSCVEAPAPICANDTIFCDVYPEKPSIRDQYVDYNHPKLTDSENLITDVLALSTTDGSSSGPLCPISDIPFSCAKTDFLRGLARNVIGLAALGRSNESIPSMISAKFNSPRIFAVCLSGTRSGSGVAFFGSRGPYGFFPNVDLSKSLIYTPLLFNPVSGSIYTWWLPSYEYYIGLSAIRINGKAVPFNTSLLAFEPVHGGGGTKISTSTTYALLQTSIYRGFATAFMKEAAALNFTLTNAVEPFGVCFAADSVAVTAAGPMAPAVDLVMENGKVVWKLGGRNSMVRIKKNGVDAWCLGFINGGEFPRTPIVIGGLQMEDHLLQFDLENYRFGFSSSALLKGTSCSNFNFTSAQSFL
Homology
BLAST of Spg014158 vs. NCBI nr
Match: XP_022959602.1 (basic 7S globulin 2-like [Cucurbita moschata])

HSP 1 Score: 729.6 bits (1882), Expect = 1.7e-206
Identity = 367/450 (81.56%), Postives = 394/450 (87.56%), Query Frame = 0

Query: 1   MGTRASILFFIFFLLSFPFLSLQTAFVAPILKDHNSLLYSISVHLKTPLRPANLHLDLGG 60
           M T    +FF F LLSFPFLSLQTAF+API KDHNS LYSISVHLKTPLRPANLHLDLGG
Sbjct: 1   MATPLFFVFFTFSLLSFPFLSLQTAFIAPIHKDHNSGLYSISVHLKTPLRPANLHLDLGG 60

Query: 61  AFSWIDCYNRYNSSSYRLVKCDSALCNSMDSKVCGSCVEAPAPICANDTIFCDVYPEKPS 120
           AFSWIDCYN YNSSSYRLVKC+S L +S +  VCGSC++AP PICANDTIF  VYPEKPS
Sbjct: 61  AFSWIDCYNHYNSSSYRLVKCNSPLSDSFNHGVCGSCIQAPTPICANDTIFSYVYPEKPS 120

Query: 121 IRDQYVDYNHPKLTDSENLITDVLALSTTDGSSSGPLCPISDIPFSCAKTDFLRGLARNV 180
           IRD+YVDY + KLTDSEN++TDVLALSTTDGS SG L  IS++PF+C KT+FLRGLARNV
Sbjct: 121 IRDEYVDYLNAKLTDSENVVTDVLALSTTDGSRSGSLRRISNMPFACVKTNFLRGLARNV 180

Query: 181 IGLAALGRSNESIPSMISAKFNSPRIFAVCLSGTRSGSGVAFFGSRGPYGFFPNVDLSKS 240
           IGLAALGR+NESIP  ISAKFNSPRIFA+CLSGTR GSGVAF GS+GPY F PNVDLSKS
Sbjct: 181 IGLAALGRANESIPLTISAKFNSPRIFAICLSGTRLGSGVAFIGSKGPYAFSPNVDLSKS 240

Query: 241 LIYTPLLFNPVSGSIYTWWLPSYEYYIGLSAIRINGKAVPFNTSLLAFEPVHGGGGTKIS 300
           LIYTPLLFNP SGSIYT WLPSYEYYIGLSAIRIN +AV FNTSLL FEPVHG GG KIS
Sbjct: 241 LIYTPLLFNPQSGSIYTNWLPSYEYYIGLSAIRINNEAVRFNTSLLQFEPVHGRGGAKIS 300

Query: 301 TSTTYALLQTSIYRGFATAFMKEAAALNFTLTNAVEPFGVCFAADSVAVTAAGPMAPAVD 360
           TSTTYALLQ+SIYR FA AFMKEAA LNFTL NAVEPFGVCF   SV +TAAGP AP V 
Sbjct: 301 TSTTYALLQSSIYRAFAMAFMKEAAMLNFTLANAVEPFGVCFEGSSVEMTAAGPRAPVVY 360

Query: 361 LVMENGKVVWKLGGRNSMVRIKKNGVDAWCLGFINGGEFPRTPIVIGGLQMEDHLLQFDL 420
           L ME GKVVWKLGGRNSMVRIKK GVD WCLG++NGGEFPRTPIVIGGLQMEDHLLQFDL
Sbjct: 361 LEMEKGKVVWKLGGRNSMVRIKKLGVDLWCLGYVNGGEFPRTPIVIGGLQMEDHLLQFDL 420

Query: 421 ENYRFGFSSSALLKGTSCSNFNFTSAQSFL 451
           E YRFGFSSSALL+GTSCS FNF+S+ +FL
Sbjct: 421 EKYRFGFSSSALLQGTSCSKFNFSSSDNFL 450

BLAST of Spg014158 vs. NCBI nr
Match: XP_038897313.1 (probable aspartic proteinase GIP1 [Benincasa hispida])

HSP 1 Score: 726.5 bits (1874), Expect = 1.4e-205
Identity = 363/438 (82.88%), Postives = 386/438 (88.13%), Query Frame = 0

Query: 9   FFIFFLLSFPFLSLQTAFVAPILKDHNSLLYSISVHLKTPLRPANLHLDLGGAFSWIDCY 68
           FF FFLL FP  SLQTAFVAPI KDH SLLYSISVHLKTPLRPANLHLDLGG FSWIDCY
Sbjct: 7   FFFFFLLFFPLYSLQTAFVAPIYKDHISLLYSISVHLKTPLRPANLHLDLGGGFSWIDCY 66

Query: 69  NRYNSSSYRLVKCDSALCNSMDSKVCGSCVEAPAPICANDTIFCDVYPEKPSIRDQYVDY 128
           NRYNSSSY+ V C+S L +S+    CGSCVEAP+P+CANDTIF   YPEKP +RDQ+VDY
Sbjct: 67  NRYNSSSYQFVLCNSPLSHSLQQNTCGSCVEAPSPVCANDTIFSYAYPEKPFLRDQFVDY 126

Query: 129 NHPKLTDSENLITDVLALSTTDGSSSGPLCPISDIPFSCAKTDFLRGLARNVIGLAALGR 188
           NH KLTDSEN+ITDVLAL TTDGS S PL  I +IPFSC KTDFLRGLAR VIGLAALGR
Sbjct: 127 NHAKLTDSENVITDVLALFTTDGSDSYPLRRIPEIPFSCVKTDFLRGLARGVIGLAALGR 186

Query: 189 SNESIPSMISAKFNSPRIFAVCLSGTRSGSGVAFFGSRGPYGFFPNVDLSKSLIYTPLLF 248
           SN SIPS+ISAKFNSPR FA+CLSG R G+GVAFFGS+GPY FFPNVDLSKSLIYTPLLF
Sbjct: 187 SNVSIPSVISAKFNSPRFFAICLSGARLGTGVAFFGSKGPYKFFPNVDLSKSLIYTPLLF 246

Query: 249 NPVSGSIYTWWLPSYEYYIGLSAIRINGKAVPFNTSLLAFEPVHGGGGTKISTSTTYALL 308
           +P + SIYT WLPSYEYYIGLSAIRINGKAVPFNTSLL+FEPV GGGGTKISTST YALL
Sbjct: 247 SPATNSIYTNWLPSYEYYIGLSAIRINGKAVPFNTSLLSFEPVIGGGGTKISTSTNYALL 306

Query: 309 QTSIYRGFATAFMKEAAALNFTLTNAVEPFGVCFAADSVAVTAAG-PMAPAVDLVMENGK 368
           Q+SIYR F T FMKE+AALNFTLTNAVEPFGVC+ A SV VTA G   AP VDLVME GK
Sbjct: 307 QSSIYRAFTTVFMKESAALNFTLTNAVEPFGVCYTAYSVGVTAEGQARAPVVDLVMEKGK 366

Query: 369 VVWKLGGRNSMVRIKKNGVDAWCLGFINGGEFPRTPIVIGGLQMEDHLLQFDLENYRFGF 428
           VVWKLGGRN+MVRIKKNGVD WCLGFINGGEFPRTPIVIGGLQMEDHLLQFDLENYRFGF
Sbjct: 367 VVWKLGGRNTMVRIKKNGVDVWCLGFINGGEFPRTPIVIGGLQMEDHLLQFDLENYRFGF 426

Query: 429 SSSALLKGTSCSNFNFTS 446
           SSSAL++GTSCS FNFTS
Sbjct: 427 SSSALMEGTSCSKFNFTS 444

BLAST of Spg014158 vs. NCBI nr
Match: KAG7027263.1 (hypothetical protein SDJN02_11275, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 724.2 bits (1868), Expect = 7.1e-205
Identity = 363/450 (80.67%), Postives = 394/450 (87.56%), Query Frame = 0

Query: 1   MGTRASILFFIFFLLSFPFLSLQTAFVAPILKDHNSLLYSISVHLKTPLRPANLHLDLGG 60
           M T    +FF F LLSFPFLSLQTAF+API KDHNS LYSISVH+KTPLRPANLHLDLGG
Sbjct: 1   MATPLFFVFFTFSLLSFPFLSLQTAFIAPIHKDHNSGLYSISVHVKTPLRPANLHLDLGG 60

Query: 61  AFSWIDCYNRYNSSSYRLVKCDSALCNSMDSKVCGSCVEAPAPICANDTIFCDVYPEKPS 120
           AFSWIDCYN YNSSSYRLVKC+S L +S + +VC SC++AP  ICAND IF DVYPEKPS
Sbjct: 61  AFSWIDCYNHYNSSSYRLVKCNSPLSDSFNHRVCSSCIQAPTSICANDAIFSDVYPEKPS 120

Query: 121 IRDQYVDYNHPKLTDSENLITDVLALSTTDGSSSGPLCPISDIPFSCAKTDFLRGLARNV 180
           IRD+YVDY +PKLTDSEN++TDVLALSTTDGS  G L  IS++PF+C KT+FLRGLARNV
Sbjct: 121 IRDEYVDYLNPKLTDSENVVTDVLALSTTDGSRPGLLRRISNMPFACVKTNFLRGLARNV 180

Query: 181 IGLAALGRSNESIPSMISAKFNSPRIFAVCLSGTRSGSGVAFFGSRGPYGFFPNVDLSKS 240
           IGLAALGR+NESIP  ISAKFNSPRIFA+CLSGTR GSGVAF GS+GPY F PNVDLSKS
Sbjct: 181 IGLAALGRANESIPLTISAKFNSPRIFAICLSGTRLGSGVAFIGSKGPYVFSPNVDLSKS 240

Query: 241 LIYTPLLFNPVSGSIYTWWLPSYEYYIGLSAIRINGKAVPFNTSLLAFEPVHGGGGTKIS 300
           LIYTPLLFNP SGSIYT WLPSYEYYIGLSAIRIN +AV FNTSLL FEPVHG GG KIS
Sbjct: 241 LIYTPLLFNPQSGSIYTNWLPSYEYYIGLSAIRINNEAVRFNTSLLQFEPVHGRGGAKIS 300

Query: 301 TSTTYALLQTSIYRGFATAFMKEAAALNFTLTNAVEPFGVCFAADSVAVTAAGPMAPAVD 360
           TSTTYALL++SIYR FATAFMKEAA LNFTL NAVEPFGVCF   SVA+TAAGP AP V 
Sbjct: 301 TSTTYALLESSIYRAFATAFMKEAAMLNFTLANAVEPFGVCFEGSSVAMTAAGPRAPVVY 360

Query: 361 LVMENGKVVWKLGGRNSMVRIKKNGVDAWCLGFINGGEFPRTPIVIGGLQMEDHLLQFDL 420
           L ME GKVVWKLGGRNSMVRIKK GVD WCLG++NGGEFPRTPIVIGGLQMEDHLLQFDL
Sbjct: 361 LEMEKGKVVWKLGGRNSMVRIKKLGVDLWCLGYVNGGEFPRTPIVIGGLQMEDHLLQFDL 420

Query: 421 ENYRFGFSSSALLKGTSCSNFNFTSAQSFL 451
           E +RFGFSSSALL+GTSCS FNF+S+ + L
Sbjct: 421 EKHRFGFSSSALLQGTSCSKFNFSSSDNLL 450

BLAST of Spg014158 vs. NCBI nr
Match: XP_023004536.1 (basic 7S globulin 2-like [Cucurbita maxima])

HSP 1 Score: 723.4 bits (1866), Expect = 1.2e-204
Identity = 362/450 (80.44%), Postives = 391/450 (86.89%), Query Frame = 0

Query: 1   MGTRASILFFIFFLLSFPFLSLQTAFVAPILKDHNSLLYSISVHLKTPLRPANLHLDLGG 60
           M T    +FF F LLSFPF SLQTAF+API KDHNSLLYSISVH+KTPLRPANLHLDLGG
Sbjct: 1   MATPLFFVFFTFSLLSFPFFSLQTAFIAPIHKDHNSLLYSISVHVKTPLRPANLHLDLGG 60

Query: 61  AFSWIDCYNRYNSSSYRLVKCDSALCNSMDSKVCGSCVEAPAPICANDTIFCDVYPEKPS 120
           AFSWIDCYN YNSSSYRLVKC+S L +S +  VCGSC++AP P C NDTIF  VYPEKPS
Sbjct: 61  AFSWIDCYNHYNSSSYRLVKCNSPLSDSFNHGVCGSCIQAPTPTCGNDTIFTYVYPEKPS 120

Query: 121 IRDQYVDYNHPKLTDSENLITDVLALSTTDGSSSGPLCPISDIPFSCAKTDFLRGLARNV 180
           IRD+YVDY + KLTDSEN++TDVLALSTTDGS SG L  +S +PF+C KT+FLRGLARNV
Sbjct: 121 IRDEYVDYLNAKLTDSENVVTDVLALSTTDGSRSGSLRQVSYMPFACVKTNFLRGLARNV 180

Query: 181 IGLAALGRSNESIPSMISAKFNSPRIFAVCLSGTRSGSGVAFFGSRGPYGFFPNVDLSKS 240
           IGLAALGR+NESIP  ISAKFNSPRIFA+CLSGTR GSGVAFFGS+GPY F PNVDLSKS
Sbjct: 181 IGLAALGRANESIPLTISAKFNSPRIFAICLSGTRLGSGVAFFGSKGPYIFSPNVDLSKS 240

Query: 241 LIYTPLLFNPVSGSIYTWWLPSYEYYIGLSAIRINGKAVPFNTSLLAFEPVHGGGGTKIS 300
           LIYTPLLFNP SGSIYT WLPSYEYYIGLSAIRIN + V FNTSLL FEPVHG GG KIS
Sbjct: 241 LIYTPLLFNPQSGSIYTNWLPSYEYYIGLSAIRINNEVVRFNTSLLQFEPVHGRGGAKIS 300

Query: 301 TSTTYALLQTSIYRGFATAFMKEAAALNFTLTNAVEPFGVCFAADSVAVTAAGPMAPAVD 360
           TSTTYALLQ+SIYR FA AFMKEAA LNFTL NAVEPFGVCF   SV +TAAGP AP V 
Sbjct: 301 TSTTYALLQSSIYRAFAMAFMKEAARLNFTLANAVEPFGVCFEGSSVEMTAAGPRAPVVY 360

Query: 361 LVMENGKVVWKLGGRNSMVRIKKNGVDAWCLGFINGGEFPRTPIVIGGLQMEDHLLQFDL 420
           L ME GKVVWKLGGRNSMVRIKK GVD WCLG++NGGEFPRTPIVIGGLQMEDHLLQFDL
Sbjct: 361 LEMEKGKVVWKLGGRNSMVRIKKLGVDLWCLGYVNGGEFPRTPIVIGGLQMEDHLLQFDL 420

Query: 421 ENYRFGFSSSALLKGTSCSNFNFTSAQSFL 451
           E YRFGFSSSAL++GTSCS FNF+S+ +FL
Sbjct: 421 EKYRFGFSSSALMQGTSCSKFNFSSSDNFL 450

BLAST of Spg014158 vs. NCBI nr
Match: XP_023514746.1 (basic 7S globulin 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 723.0 bits (1865), Expect = 1.6e-204
Identity = 362/450 (80.44%), Postives = 394/450 (87.56%), Query Frame = 0

Query: 1   MGTRASILFFIFFLLSFPFLSLQTAFVAPILKDHNSLLYSISVHLKTPLRPANLHLDLGG 60
           M T    +FF F LL FPFLSLQTAF+API KDHNS LYSISVH+KTPLRPANLHLDLGG
Sbjct: 1   MATPLFFVFFTFSLLPFPFLSLQTAFIAPIHKDHNSGLYSISVHVKTPLRPANLHLDLGG 60

Query: 61  AFSWIDCYNRYNSSSYRLVKCDSALCNSMDSKVCGSCVEAPAPICANDTIFCDVYPEKPS 120
           AFSWIDCYN YNSSSYRLVKC+S L +S + +VC SC++AP  ICAND IF DVYPEKPS
Sbjct: 61  AFSWIDCYNHYNSSSYRLVKCNSPLSDSFNHRVCSSCIQAPTSICANDAIFSDVYPEKPS 120

Query: 121 IRDQYVDYNHPKLTDSENLITDVLALSTTDGSSSGPLCPISDIPFSCAKTDFLRGLARNV 180
           IRD+YVDY +PKLTDSEN++TDVLALSTTDGS SG L  IS++PF+C KT+FLRGLARNV
Sbjct: 121 IRDEYVDYLNPKLTDSENVVTDVLALSTTDGSRSGLLRRISNMPFACVKTNFLRGLARNV 180

Query: 181 IGLAALGRSNESIPSMISAKFNSPRIFAVCLSGTRSGSGVAFFGSRGPYGFFPNVDLSKS 240
           IGLAALGR+NESIP  ISAKFNSPRIFA+CLSGTR GSGVAF GS+GPY F PNVDLSKS
Sbjct: 181 IGLAALGRANESIPLTISAKFNSPRIFAICLSGTRLGSGVAFIGSKGPYVFSPNVDLSKS 240

Query: 241 LIYTPLLFNPVSGSIYTWWLPSYEYYIGLSAIRINGKAVPFNTSLLAFEPVHGGGGTKIS 300
           LIYTPLLFNP SGSIYT WLPSYEYYIGLSAIRIN +AV FNTSLL FEPVHG GG KIS
Sbjct: 241 LIYTPLLFNPQSGSIYTNWLPSYEYYIGLSAIRINNEAVRFNTSLLQFEPVHGRGGAKIS 300

Query: 301 TSTTYALLQTSIYRGFATAFMKEAAALNFTLTNAVEPFGVCFAADSVAVTAAGPMAPAVD 360
           TSTTYALL++SIYR FATAFMKEAA LNFTL NAVEPFGVCF   SVA+TAAGP AP V 
Sbjct: 301 TSTTYALLESSIYRAFATAFMKEAAMLNFTLANAVEPFGVCFEGSSVAMTAAGPRAPVVY 360

Query: 361 LVMENGKVVWKLGGRNSMVRIKKNGVDAWCLGFINGGEFPRTPIVIGGLQMEDHLLQFDL 420
           L ME GKVVWKLGGRNSMVRIKK GVD WCLG++NGGEFPRTPIVIGGLQMEDHLLQFDL
Sbjct: 361 LEMEKGKVVWKLGGRNSMVRIKKLGVDLWCLGYVNGGEFPRTPIVIGGLQMEDHLLQFDL 420

Query: 421 ENYRFGFSSSALLKGTSCSNFNFTSAQSFL 451
           E +RFGF+SSALL+GTSCS FNF+S+ + L
Sbjct: 421 EKHRFGFTSSALLQGTSCSKFNFSSSDNLL 450

BLAST of Spg014158 vs. ExPASy Swiss-Prot
Match: I1JNS6 (Probable aspartic proteinase GIP1 OS=Glycine max OX=3847 GN=GIP1 PE=1 SV=2)

HSP 1 Score: 352.8 bits (904), Expect = 5.6e-96
Identity = 199/432 (46.06%), Postives = 267/432 (61.81%), Query Frame = 0

Query: 11  IFFLLSFPFLSLQTAFVAPILKDHNSLLYSISVHLKTPLRPANLHLDLGGAFSWIDCYNR 70
           I FL   P  + Q   +API KD  + LY++SV LKTPL+P  LHL LG + SW+ C + 
Sbjct: 15  ILFLFLTP--TFQIPLIAPISKDDTTQLYTLSVFLKTPLQPTKLHLHLGSSLSWVLCDST 74

Query: 71  YNSSSYRLVKCDSALCNSMDSKVCGSCVEAPAPICANDTIFCDVYPEKPSIRDQYVDYNH 130
           Y SSS   + C++ LCNS            P+  C+N++  C ++PE P  R+  +D   
Sbjct: 75  YTSSSSHHIPCNTPLCNSF-----------PSNACSNNSSLCALFPENPVTRNTLLD--- 134

Query: 131 PKLTDSENLITDVLALSTTDGSSSGPLCPISDIPFSCAKTDFLRGLARNVIGLAALGRSN 190
                    + D LAL T D SSS  L  ISD  FSCA    L+GLA N +GLA+LGRSN
Sbjct: 135 -------TALIDSLALPTYDASSS--LVLISDFIFSCATAHLLQGLAANALGLASLGRSN 194

Query: 191 ESIPSMISAKFNSPRIFAVCLSGTRSGSGVAFFGS-RGPYGFFPNVDLSKSLIYTPLLFN 250
            S+P+ IS    SPR F +CL  + + +G A F S    + F   +DL+    YT L+ N
Sbjct: 195 YSLPAQISTSLTSPRSFTLCLPASSANTGAAIFASTASSFLFSSKIDLT----YTQLIVN 254

Query: 251 PVSGSIYT-WWLPSYEYYIGLSAIRINGKAVPFNTSLLAFEPVHGGGGTKISTSTTYALL 310
           PV+ ++ T    PS EY+I L++I+INGK +  N+S+L  +   G GGTKIST+  Y +L
Sbjct: 255 PVADTVVTDNPQPSDEYFINLTSIKINGKPLYINSSILTVDQT-GFGGTKISTAEPYTVL 314

Query: 311 QTSIYRGFATAFMKEAAALNFTLTNAVEPFGVCFAADSVAVTAAGPMAPAVDLVMENGKV 370
           +TSIYR F   F+ E++A N T+T AVEPFGVC+ A  +  T  GP  P VDLVM +  V
Sbjct: 315 ETSIYRLFVQRFVNESSAFNLTVTEAVEPFGVCYPAGDLTETRVGPAVPTVDLVMHSEDV 374

Query: 371 VWKLGGRNSMVRIKKNGVDAWCLGFINGGEFPRTPIVIGGLQMEDHLLQFDLENYRFGFS 430
            W++ G NSMVR+ K GVD WCLGF++GG   RTPIVIGG Q+ED+L+QFDL++ RFGF+
Sbjct: 375 FWRIFGGNSMVRVAKGGVDVWCLGFVDGGTRGRTPIVIGGHQLEDNLMQFDLDSNRFGFT 416

Query: 431 SSALLKGTSCSN 441
           S+ LL+   CSN
Sbjct: 435 STLLLQDAKCSN 416

BLAST of Spg014158 vs. ExPASy Swiss-Prot
Match: P0DO21 (Probable aspartic proteinase GIP2 OS=Nicotiana benthamiana OX=4100 GN=GIP2 PE=1 SV=1)

HSP 1 Score: 323.9 bits (829), Expect = 2.8e-87
Identity = 184/422 (43.60%), Postives = 249/422 (59.00%), Query Frame = 0

Query: 27  VAPILKDHNSLLYSISVHLKTPLRPANLHLDLGGAFSWIDCYNRYNSSSYRLVKCDSALC 86
           + PI KD  +L Y   +  +TPL P +L LDLGG F W+DC   Y SS+YR  +C SA C
Sbjct: 35  ILPITKDALTLQYLTQIQQRTPLVPVSLTLDLGGQFLWVDCDQGYVSSTYRPARCRSAQC 94

Query: 87  NSMDS-KVCGSCVEAPAPICANDTIFCDVYPEKPSIRDQYVDYNHPKLTDSENLITDVLA 146
           +   +   CG C   P P C N+T  C + P+    R             S  L +D + 
Sbjct: 95  SLAGAGSGCGQCFSPPKPGCNNNT--CGLLPDNTITR----------TATSGELASDTVQ 154

Query: 147 LSTTDGSSSGPLCPISDIPFSCAKTDFLRGLARNVIGLAALGRSNESIPSMISAKFNSPR 206
           + +++G + G      D  F C  T  L GLA  V G+A LGR+  S+PS  SA+F+ PR
Sbjct: 155 VQSSNGKNPGRHVSDKDFLFVCGSTFLLEGLASGVKGMAGLGRTRISLPSQFSAEFSFPR 214

Query: 207 IFAVCLSGTRSGSGVAFFGSRGPYGFFPNVDLSKS-LIYTPLLFNPVS-GSIYTWWLPSY 266
            FAVCLS + +  GV  FG  GPY F PN + + +   YTPL  NPVS  S ++   PS 
Sbjct: 215 KFAVCLSSSTNSKGVVLFGD-GPYTFLPNREFANNDFSYTPLFINPVSTASAFSSREPSS 274

Query: 267 EYYIGLSAIRINGKAVPFNTSLLAFEPVHGGGGTKISTSTTYALLQTSIYRGFATAFMKE 326
           EY+IG+ +I+IN K VP NT+LL+ +   G GGTKIST   Y +L+TSIY      F+KE
Sbjct: 275 EYFIGVKSIKINEKVVPINTTLLSIDN-QGVGGTKISTVNPYTILETSIYNAVTNFFVKE 334

Query: 327 AAALNFTLTNAVEPFGVCFAADSVAVTAAGPMAPAVDLVMENGKVVWKLGGRNSMVRIKK 386
              +N T   +V PF  CF + ++A T  GP  P++DLV++N  V W++ G NSMV++ +
Sbjct: 335 --LVNITRVASVAPFRACFDSRNIASTRVGPAVPSIDLVLQNENVFWRIFGANSMVQVSE 394

Query: 387 NGVDAWCLGFINGGEFPRTPIVIGGLQMEDHLLQFDLENYRFGFSSSALLKGTSCSNFNF 446
           N     CLGF++GG  PRT IV+GG  +ED+LLQFDL   R GF+SS L + T+C+NFNF
Sbjct: 395 N---VLCLGFVDGGVSPRTSIVVGGYTIEDNLLQFDLARSRLGFTSSILFRQTTCANFNF 437

BLAST of Spg014158 vs. ExPASy Swiss-Prot
Match: P82952 (Gamma conglutin 1 OS=Prunus dulcis OX=3755 GN=Cgamma1 PE=1 SV=2)

HSP 1 Score: 244.2 bits (622), Expect = 2.8e-63
Identity = 150/425 (35.29%), Postives = 223/425 (52.47%), Query Frame = 0

Query: 27  VAPILKDHNSLLYSISVHLKTPLRPANLHLDLGGAFSWIDCYNRYNSSSYRLVKCDSALC 86
           V  + KD  + L+ + +H +TPL      +DL G F  ++C N+Y SS+Y+   C S+ C
Sbjct: 39  VLKVQKDRATNLHVVQIHKRTPLVQFPFVIDLTGRFLSVNCENQYTSSTYKAPVCHSSQC 98

Query: 87  NSMDSKVCGSCVEAPA-PICANDTIFCDVYPEKPSIRDQYVDYNHPKLTDSENLITDVLA 146
              +S  C +C  +   P C   T  C +    P  +           +    L  DVL 
Sbjct: 99  ARANSHTCRTCSSSKTRPGC--HTNACGLLTTNPVTQQ----------SAQGELAEDVLK 158

Query: 147 LSTTDGSSSGPLCPISDIPFSCAKTDFL-RGLARNVIGLAALGRSNESIPSMISAKFNSP 206
           + +T GSS GP+       F+CA ++ L +GL +NV G+A LG S  S+P  +++ F  P
Sbjct: 159 IPSTQGSSPGPMVTYPHFLFACAPSNILQKGLPKNVQGVAGLGHSPISLPYQLASHFGFP 218

Query: 207 RIFAVCLSGTRSGSGVAFFGSRGPYGFFPNVDLSKSLIYTPLLFNPVSGSIYTWWLPSYE 266
             FAVCL+ +   +G  FFG  GPY   P +D+S+ L Y P                  E
Sbjct: 219 PKFAVCLTSSPGKNGAVFFG-EGPYFMKPGIDVSRQLTYAPFTIGQQG-----------E 278

Query: 267 YYIGLSAIRINGKAVPFNTSLLAFEPVHGGGGTKISTSTTYALLQTSIYRGFATAFMKEA 326
           YYI + + +I       N ++L   P  G GG  IST+T Y  LQT I+R     FM + 
Sbjct: 279 YYINVQSFKI-------NNAMLPSIPKGGFGGAMISTTTPYTTLQTPIFRALNQLFMNQL 338

Query: 327 AALNFTLTNAVEPFGVCFAADSVAVTAAGPMAPAVDLVMENGK-VVWKLGGRNSMVRIKK 386
             +       V PFG CF A+ +  +  GP  P++DLV++N K ++W++ G N+M++ + 
Sbjct: 339 RGVPH--VKPVAPFGACFDANRIPTSKMGPTVPSIDLVLDNKKNIMWRIFGANAMIQPRP 398

Query: 387 NGVDAWCLGFINGGEFPRTPIVIGGLQMEDHLLQFDLENYRFGFSSSALLKGTSCSNFNF 446
                 CL F++GG  P+ PIVIG  Q+ED+LLQFDL N R GFSSS L + T+C+NFNF
Sbjct: 399 G---VMCLAFVDGGMRPKAPIVIGTQQLEDNLLQFDLMNSRLGFSSSLLFRRTNCANFNF 427

Query: 447 TSAQS 449
            ++ +
Sbjct: 459 GTSST 427

BLAST of Spg014158 vs. ExPASy Swiss-Prot
Match: Q8RVH5 (Basic 7S globulin 2 OS=Glycine max OX=3847 PE=1 SV=1)

HSP 1 Score: 188.3 bits (477), Expect = 1.8e-46
Identity = 151/469 (32.20%), Postives = 225/469 (47.97%), Query Frame = 0

Query: 5   ASIL-FFIFFLLSFPFLSLQT-----------------AFVAPILKDHNSLLYSISVHLK 64
           ASIL +F+   LSF FL   +                   V P+  D ++ L+  ++  +
Sbjct: 2   ASILHYFLALSLSFSFLFFLSDSVPIPQHHTNPTKPINLLVLPVQNDASTGLHWANLQKR 61

Query: 65  TPLRPANLHLDLGGAFSWIDCYNRYNSSSYRLVKCDSALCNSMDSKVCGSCVEAPAPICA 124
           TPL    + +DL G   W++C   Y+S +Y+   C S  C+  ++  C SC  A  P C 
Sbjct: 62  TPLMQVPVLVDLNGNHLWVNCEQHYSSKTYQAPFCHSTQCSRANTHQCLSCPAASRPGCH 121

Query: 125 NDTIFCDVYPEKPSIRDQYVDYNHPKLTDSENLITDVLALSTTDGSSS--GPLCPISDIP 184
            +T  C +    P  +           T    L  DVLA+  T GS+   GPL  +    
Sbjct: 122 KNT--CGLMSTNPITQQ----------TGLGELGQDVLAIHATQGSTQQLGPLVTVPQFL 181

Query: 185 FSCAKTDFL-RGLARNVIGLAALGRSNESIPSMISAKFNSPRIFAVCLSGTRSGSGVAFF 244
           FSCA +  L +GL RN+ G+A LG +  S+P+ +++ F     F  CLS   +  G   F
Sbjct: 182 FSCAPSFLLQKGLPRNIQGVAGLGHAPISLPNQLASHFGLQHQFTTCLSRYPTSKGALIF 241

Query: 245 GSRGPYGF--FPNVDLSKSLIYTPLLFNPVSGSIYTWWLPSYEYYIGLSAIRINGKAVPF 304
           G   P     F N D+   L +TPL   P             EY + +S+IRIN  +V F
Sbjct: 242 GD-APNNMQQFHNQDIFHDLAFTPLTVTPQG-----------EYNVRVSSIRINQHSV-F 301

Query: 305 NTSLLAFEPVHGGGGTKISTSTTYALLQTSIYRGFATAFMKEAAALNFTLTNAVEPFGVC 364
             + ++   V   GGT ISTST + +LQ S+Y+ F   F ++          +V PFG+C
Sbjct: 302 PPNKISSTIVGSSGGTMISTSTPHMVLQQSLYQAFTQVFAQQLE--KQAQVKSVAPFGLC 361

Query: 365 FAADSVAVTAAGPMAPAVDLVME--NGKVVWKLGGRNSMVRIKKNGVDAWCLGFINGGEF 424
           F ++ +         P+VDLVM+  NG  VW++ G + MV+ +       CLG +NGG  
Sbjct: 362 FNSNKINA------YPSVDLVMDKPNGP-VWRISGEDLMVQAQPG---VTCLGVMNGGMQ 421

Query: 425 PRTPIVIGGLQMEDHLLQFDLENYRFGFSSSAL-LKGTSCSN-FNFTSA 447
           PR  + +G  Q+E+ L+ FDL   R GFS+S+L   G  C + FNF +A
Sbjct: 422 PRAEVTLGTRQLEEKLMVFDLARSRVGFSTSSLHSHGVKCGDLFNFANA 433

BLAST of Spg014158 vs. ExPASy Swiss-Prot
Match: P13917 (Basic 7S globulin OS=Glycine max OX=3847 GN=BG PE=1 SV=2)

HSP 1 Score: 186.8 bits (473), Expect = 5.3e-46
Identity = 139/429 (32.40%), Postives = 213/429 (49.65%), Query Frame = 0

Query: 27  VAPILKDHNSLLYSISVHLKTPLRPANLHLDLGGAFSWIDCYNRYNSSSYRLVKCDSALC 86
           V P+  D ++ L+  ++  +TPL    + +DL G   W++C  +Y+S +Y+   C S  C
Sbjct: 35  VLPVQNDGSTGLHWANLQKRTPLMQVPVLVDLNGNHLWVNCEQQYSSKTYQAPFCHSTQC 94

Query: 87  NSMDSKVCGSCVEAPAPICANDTIFCDVYPEKPSIRDQYVDYNHPKLTDSENLITDVLAL 146
           +  ++  C SC  A  P C  +T  C +    P  +           T    L  DVLA+
Sbjct: 95  SRANTHQCLSCPAASRPGCHKNT--CGLMSTNPITQQ----------TGLGELGEDVLAI 154

Query: 147 STTDGSSS--GPLCPISDIPFSCAKTDFL-RGLARNVIGLAALGRSNESIPSMISAKFNS 206
             T GS+   GPL  +    FSCA +  + +GL RN  G+A LG +  S+P+ +++ F  
Sbjct: 155 HATQGSTQQLGPLVTVPQFLFSCAPSFLVQKGLPRNTQGVAGLGHAPISLPNQLASHFGL 214

Query: 207 PRIFAVCLSGTRSGSGVAFFGSRGPYGF--FPNVDLSKSLIYTPLLFNPVSGSIYTWWLP 266
            R F  CLS   +  G   FG   P     F N D+   L +TPL    + G        
Sbjct: 215 QRQFTTCLSRYPTSKGAIIFGD-APNNMRQFQNQDIFHDLAFTPLTIT-LQG-------- 274

Query: 267 SYEYYIGLSAIRINGKAVPFNTSLLAFEPVHGGGGTKISTSTTYALLQTSIYRGFATAFM 326
             EY + +++IRIN  +V     + +       GGT ISTST + +LQ S+Y+ F   F 
Sbjct: 275 --EYNVRVNSIRINQHSVFPLNKISSTIVGSTSGGTMISTSTPHMVLQQSVYQAFTQVFA 334

Query: 327 KEAAALNFTLTNAVEPFGVCFAADSVAVTAAGPMAPAVDLVME--NGKVVWKLGGRNSMV 386
           ++          +V PFG+CF ++ +         P+VDLVM+  NG  VW++ G + MV
Sbjct: 335 QQLP--KQAQVKSVAPFGLCFNSNKINA------YPSVDLVMDKPNGP-VWRISGEDLMV 394

Query: 387 RIKKNGVDAWCLGFINGGEFPRTPIVIGGLQMEDHLLQFDLENYRFGFSSSAL-LKGTSC 446
           + +       CLG +NGG  PR  I +G  Q+E++L+ FDL   R GFS+S+L   G  C
Sbjct: 395 QAQPG---VTCLGVMNGGMQPRAEITLGARQLEENLVVFDLARSRVGFSTSSLHSHGVKC 427

BLAST of Spg014158 vs. ExPASy TrEMBL
Match: A0A6J1H501 (basic 7S globulin 2-like OS=Cucurbita moschata OX=3662 GN=LOC111460627 PE=4 SV=1)

HSP 1 Score: 729.6 bits (1882), Expect = 8.2e-207
Identity = 367/450 (81.56%), Postives = 394/450 (87.56%), Query Frame = 0

Query: 1   MGTRASILFFIFFLLSFPFLSLQTAFVAPILKDHNSLLYSISVHLKTPLRPANLHLDLGG 60
           M T    +FF F LLSFPFLSLQTAF+API KDHNS LYSISVHLKTPLRPANLHLDLGG
Sbjct: 1   MATPLFFVFFTFSLLSFPFLSLQTAFIAPIHKDHNSGLYSISVHLKTPLRPANLHLDLGG 60

Query: 61  AFSWIDCYNRYNSSSYRLVKCDSALCNSMDSKVCGSCVEAPAPICANDTIFCDVYPEKPS 120
           AFSWIDCYN YNSSSYRLVKC+S L +S +  VCGSC++AP PICANDTIF  VYPEKPS
Sbjct: 61  AFSWIDCYNHYNSSSYRLVKCNSPLSDSFNHGVCGSCIQAPTPICANDTIFSYVYPEKPS 120

Query: 121 IRDQYVDYNHPKLTDSENLITDVLALSTTDGSSSGPLCPISDIPFSCAKTDFLRGLARNV 180
           IRD+YVDY + KLTDSEN++TDVLALSTTDGS SG L  IS++PF+C KT+FLRGLARNV
Sbjct: 121 IRDEYVDYLNAKLTDSENVVTDVLALSTTDGSRSGSLRRISNMPFACVKTNFLRGLARNV 180

Query: 181 IGLAALGRSNESIPSMISAKFNSPRIFAVCLSGTRSGSGVAFFGSRGPYGFFPNVDLSKS 240
           IGLAALGR+NESIP  ISAKFNSPRIFA+CLSGTR GSGVAF GS+GPY F PNVDLSKS
Sbjct: 181 IGLAALGRANESIPLTISAKFNSPRIFAICLSGTRLGSGVAFIGSKGPYAFSPNVDLSKS 240

Query: 241 LIYTPLLFNPVSGSIYTWWLPSYEYYIGLSAIRINGKAVPFNTSLLAFEPVHGGGGTKIS 300
           LIYTPLLFNP SGSIYT WLPSYEYYIGLSAIRIN +AV FNTSLL FEPVHG GG KIS
Sbjct: 241 LIYTPLLFNPQSGSIYTNWLPSYEYYIGLSAIRINNEAVRFNTSLLQFEPVHGRGGAKIS 300

Query: 301 TSTTYALLQTSIYRGFATAFMKEAAALNFTLTNAVEPFGVCFAADSVAVTAAGPMAPAVD 360
           TSTTYALLQ+SIYR FA AFMKEAA LNFTL NAVEPFGVCF   SV +TAAGP AP V 
Sbjct: 301 TSTTYALLQSSIYRAFAMAFMKEAAMLNFTLANAVEPFGVCFEGSSVEMTAAGPRAPVVY 360

Query: 361 LVMENGKVVWKLGGRNSMVRIKKNGVDAWCLGFINGGEFPRTPIVIGGLQMEDHLLQFDL 420
           L ME GKVVWKLGGRNSMVRIKK GVD WCLG++NGGEFPRTPIVIGGLQMEDHLLQFDL
Sbjct: 361 LEMEKGKVVWKLGGRNSMVRIKKLGVDLWCLGYVNGGEFPRTPIVIGGLQMEDHLLQFDL 420

Query: 421 ENYRFGFSSSALLKGTSCSNFNFTSAQSFL 451
           E YRFGFSSSALL+GTSCS FNF+S+ +FL
Sbjct: 421 EKYRFGFSSSALLQGTSCSKFNFSSSDNFL 450

BLAST of Spg014158 vs. ExPASy TrEMBL
Match: A0A6J1KSD2 (basic 7S globulin 2-like OS=Cucurbita maxima OX=3661 GN=LOC111497811 PE=4 SV=1)

HSP 1 Score: 723.4 bits (1866), Expect = 5.9e-205
Identity = 362/450 (80.44%), Postives = 391/450 (86.89%), Query Frame = 0

Query: 1   MGTRASILFFIFFLLSFPFLSLQTAFVAPILKDHNSLLYSISVHLKTPLRPANLHLDLGG 60
           M T    +FF F LLSFPF SLQTAF+API KDHNSLLYSISVH+KTPLRPANLHLDLGG
Sbjct: 1   MATPLFFVFFTFSLLSFPFFSLQTAFIAPIHKDHNSLLYSISVHVKTPLRPANLHLDLGG 60

Query: 61  AFSWIDCYNRYNSSSYRLVKCDSALCNSMDSKVCGSCVEAPAPICANDTIFCDVYPEKPS 120
           AFSWIDCYN YNSSSYRLVKC+S L +S +  VCGSC++AP P C NDTIF  VYPEKPS
Sbjct: 61  AFSWIDCYNHYNSSSYRLVKCNSPLSDSFNHGVCGSCIQAPTPTCGNDTIFTYVYPEKPS 120

Query: 121 IRDQYVDYNHPKLTDSENLITDVLALSTTDGSSSGPLCPISDIPFSCAKTDFLRGLARNV 180
           IRD+YVDY + KLTDSEN++TDVLALSTTDGS SG L  +S +PF+C KT+FLRGLARNV
Sbjct: 121 IRDEYVDYLNAKLTDSENVVTDVLALSTTDGSRSGSLRQVSYMPFACVKTNFLRGLARNV 180

Query: 181 IGLAALGRSNESIPSMISAKFNSPRIFAVCLSGTRSGSGVAFFGSRGPYGFFPNVDLSKS 240
           IGLAALGR+NESIP  ISAKFNSPRIFA+CLSGTR GSGVAFFGS+GPY F PNVDLSKS
Sbjct: 181 IGLAALGRANESIPLTISAKFNSPRIFAICLSGTRLGSGVAFFGSKGPYIFSPNVDLSKS 240

Query: 241 LIYTPLLFNPVSGSIYTWWLPSYEYYIGLSAIRINGKAVPFNTSLLAFEPVHGGGGTKIS 300
           LIYTPLLFNP SGSIYT WLPSYEYYIGLSAIRIN + V FNTSLL FEPVHG GG KIS
Sbjct: 241 LIYTPLLFNPQSGSIYTNWLPSYEYYIGLSAIRINNEVVRFNTSLLQFEPVHGRGGAKIS 300

Query: 301 TSTTYALLQTSIYRGFATAFMKEAAALNFTLTNAVEPFGVCFAADSVAVTAAGPMAPAVD 360
           TSTTYALLQ+SIYR FA AFMKEAA LNFTL NAVEPFGVCF   SV +TAAGP AP V 
Sbjct: 301 TSTTYALLQSSIYRAFAMAFMKEAARLNFTLANAVEPFGVCFEGSSVEMTAAGPRAPVVY 360

Query: 361 LVMENGKVVWKLGGRNSMVRIKKNGVDAWCLGFINGGEFPRTPIVIGGLQMEDHLLQFDL 420
           L ME GKVVWKLGGRNSMVRIKK GVD WCLG++NGGEFPRTPIVIGGLQMEDHLLQFDL
Sbjct: 361 LEMEKGKVVWKLGGRNSMVRIKKLGVDLWCLGYVNGGEFPRTPIVIGGLQMEDHLLQFDL 420

Query: 421 ENYRFGFSSSALLKGTSCSNFNFTSAQSFL 451
           E YRFGFSSSAL++GTSCS FNF+S+ +FL
Sbjct: 421 EKYRFGFSSSALMQGTSCSKFNFSSSDNFL 450

BLAST of Spg014158 vs. ExPASy TrEMBL
Match: A0A5D3D4L4 (Basic 7S globulin 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold2032G00050 PE=4 SV=1)

HSP 1 Score: 696.4 bits (1796), Expect = 7.7e-197
Identity = 342/440 (77.73%), Postives = 381/440 (86.59%), Query Frame = 0

Query: 7   ILFFIFFLLSFPFLSLQTAFVAPILKDHNSLLYSISVHLKTPLRPANLHLDLGGAFSWID 66
           + FF FFL+SFP  SLQTA VAP+ KDH SLLYSIS+HLKTPLRPA L+LDLGG FSWID
Sbjct: 6   LFFFFFFLISFPSYSLQTASVAPLFKDHISLLYSISLHLKTPLRPATLYLDLGGPFSWID 65

Query: 67  CYNRYNSSSYRLVKCDSALCNSMDSKVCGSCVEAPAPICANDTIFCDVYPEKPSIRDQYV 126
           CY  YNSSSY+L+ C++ L NS +  +CGSCV+AP+P CANDTIF   YP+ PS+RDQ+V
Sbjct: 66  CYQNYNSSSYKLLLCNTPLSNSFNQGICGSCVQAPSPTCANDTIFTYAYPQNPSLRDQFV 125

Query: 127 DYNHPKLTDSENLITDVLALSTTDGSSSGPLCPISDIPFSCAKTDFLRGLARNVIGLAAL 186
           DY+ P+LTDSEN+ITDVLALSTTDGS SGPL  IS+IPF+C KT+FLRGLA+NVIGLAAL
Sbjct: 126 DYDRPELTDSENVITDVLALSTTDGSKSGPLRRISEIPFACVKTNFLRGLAKNVIGLAAL 185

Query: 187 GRSNESIPSMISAKFNSPRIFAVCLSGTRSGSGVAFFGSRGPYGFFPNVDLSKSLIYTPL 246
           GRSN SIPS+ISAKFNSP+ FA+CLSG RSG GVAFFGS+GPY F PNVDLSKSL YTPL
Sbjct: 186 GRSNLSIPSVISAKFNSPKFFAICLSGARSGPGVAFFGSKGPYRFSPNVDLSKSLTYTPL 245

Query: 247 LFNPVSGSIYTWWLPSYEYYIGLSAIRINGKAVPFNTSLLAFEPVHGGGGTKISTSTTYA 306
           LFNP SGSI+T+WLPSYEYY+GLSAIRINGK VPFNTSLL FEP+HG GG KISTST Y 
Sbjct: 246 LFNPASGSIHTYWLPSYEYYVGLSAIRINGKVVPFNTSLLPFEPIHGNGGAKISTSTNYG 305

Query: 307 LLQTSIYRGFATAFMKEAAALNFTLTNAVEPFGVCFAADSVAVTAAG-PMAPAVDLVMEN 366
           LL++SIYR FA  FMKEAAALNF L NAV+PFGVC+AA SV VTA G   AP VDLVME 
Sbjct: 306 LLESSIYRAFARVFMKEAAALNFKLINAVKPFGVCYAAKSVGVTAEGHAKAPVVDLVMEK 365

Query: 367 GKVVWKLGGRNSMVRIKKNGVDAWCLGFINGGEFPRTPIVIGGLQMEDHLLQFDLENYRF 426
           GKVVWKLGGRN+MVRIKK GVDAWCLGFINGGEFPRTPIV+GGLQMEDHLLQFDLE +RF
Sbjct: 366 GKVVWKLGGRNTMVRIKKKGVDAWCLGFINGGEFPRTPIVMGGLQMEDHLLQFDLEKFRF 425

Query: 427 GFSSSALLKGTSCSNFNFTS 446
           GFSSSAL +GTSCS FNF S
Sbjct: 426 GFSSSALTEGTSCSKFNFNS 445

BLAST of Spg014158 vs. ExPASy TrEMBL
Match: A0A0A0K506 (Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G390050 PE=4 SV=1)

HSP 1 Score: 689.5 bits (1778), Expect = 9.4e-195
Identity = 339/443 (76.52%), Postives = 380/443 (85.78%), Query Frame = 0

Query: 7   ILFFIFFLLSFPFLSLQTAFVAPILKDHNSLLYSISVHLKTPLRPANLHLDLGGAFSWID 66
           + FF FFL+SFP  SLQTA +AP+ K H SLLYSIS+HLKTPLRPA+L+LDLGGAFSWI 
Sbjct: 6   LFFFFFFLISFPLYSLQTALIAPLYKHHTSLLYSISLHLKTPLRPASLYLDLGGAFSWIH 65

Query: 67  CYNRYNSSSYRLVKCDSALCNSMDSKVCGSCVEAPAPICANDTIFCDVYPEKPSIRDQYV 126
           CY  YNSSSY+ V C++ L NS +  +CGSCV+AP+PICANDTIF   YPE PS+RD +V
Sbjct: 66  CYQNYNSSSYKFVLCNTPLSNSFNQAICGSCVQAPSPICANDTIFSYAYPENPSLRDHFV 125

Query: 127 DYNHPKLTDSENLITDVLALSTTDGSSSGPLCPISDIPFSCAKTDFLRGLARNVIGLAAL 186
           DY+HPKLTDSEN+ITDVLALSTT GS+S PL  I + PF+C KT+FLR +A+NVIGLAAL
Sbjct: 126 DYDHPKLTDSENVITDVLALSTTGGSTSAPLRRIPEFPFACVKTNFLREVAKNVIGLAAL 185

Query: 187 GRSNESIPSMISAKFNSPRIFAVCLSGTRSGSGVAFFGSRGPYGFFPNVDLSKSLIYTPL 246
           GRSN SIPS+ISAKF+SP+ FA+CLSG RSG GVAFFGS+GPY F PNVDLSKSL YTPL
Sbjct: 186 GRSNLSIPSVISAKFSSPKYFAICLSGARSGPGVAFFGSKGPYRFSPNVDLSKSLTYTPL 245

Query: 247 LFNPVSGSIYTWWLPSYEYYIGLSAIRINGKAVPFNTSLLAFEPVHGGGGTKISTSTTYA 306
           LFNPVS SIYT+WLPSYEYY+GLSAIRINGK VPFNTSLL+FEP+HG GG KISTST YA
Sbjct: 246 LFNPVSASIYTYWLPSYEYYVGLSAIRINGKVVPFNTSLLSFEPIHGRGGAKISTSTNYA 305

Query: 307 LLQTSIYRGFATAFMKEAAALNFTLTNAVEPFGVCFAADSVAVTAAG-PMAPAVDLVMEN 366
           LL++SIYR FAT FMKEA  LNF L NAVEPFGVC+ A SV VTA G   AP VDLVME 
Sbjct: 306 LLRSSIYRAFATVFMKEAVVLNFKLINAVEPFGVCYEAKSVGVTAEGQAKAPVVDLVMEK 365

Query: 367 GKVVWKLGGRNSMVRIKKNGVDAWCLGFINGGEFPRTPIVIGGLQMEDHLLQFDLENYRF 426
            KVVWKLGGRN+MVRIKK GVDAWCLGFINGGEFPRTPIVIGGLQMEDHLLQFDLEN+RF
Sbjct: 366 EKVVWKLGGRNTMVRIKKKGVDAWCLGFINGGEFPRTPIVIGGLQMEDHLLQFDLENFRF 425

Query: 427 GFSSSALLKGTSCSNFNFTSAQS 449
           GFSSSAL +GTSCS F+FTSA +
Sbjct: 426 GFSSSALKEGTSCSKFDFTSANN 448

BLAST of Spg014158 vs. ExPASy TrEMBL
Match: A0A6J1D7R8 (LOW QUALITY PROTEIN: basic 7S globulin 2 OS=Momordica charantia OX=3673 GN=LOC111018418 PE=4 SV=1)

HSP 1 Score: 599.4 bits (1544), Expect = 1.3e-167
Identity = 304/409 (74.33%), Postives = 334/409 (81.66%), Query Frame = 0

Query: 45  LKTPLRPANLHLDLGGAFSWIDCYNRYNSSSYRLVKCDSALCNSMDSKVCGSCVEAPAPI 104
           +KTPLRP  LHLDLGGAFSWIDCYN YNSSSYRLV  +SALCNSM   + G CV+AP P 
Sbjct: 1   MKTPLRPTKLHLDLGGAFSWIDCYNHYNSSSYRLVDSESALCNSMHPNIAGPCVDAPTPF 60

Query: 105 CAND--TIFCDVYPEK-PSIRDQYVDYNHPKLTDSENLITDVLALSTTDGSSSGPLCPIS 164
           C+N+  T+FC VYPEK PSIRD  VDY+H KLTDSENLI DVLALSTTDGS+ GPL  I 
Sbjct: 61  CSNNGTTLFCYVYPEKPPSIRDS-VDYDHVKLTDSENLIVDVLALSTTDGSNPGPLRQIP 120

Query: 165 DIPFSCAKTDFLRGLARNVIGLAALGRSNESIPSMISAKFNSPRIFAVCLSGTRSGSGVA 224
            +P SC           +VIGL ALG +NESIPSM+S  F   + FAVCL G RSG GVA
Sbjct: 121 HLPISC----------XDVIGLVALGXANESIPSMVSTTFGRSKYFAVCLPGARSGPGVA 180

Query: 225 FFGSRGPYGFFPNVDLSKSLIYTPLLFNPVSGSIYTWWLPSYEYYIGLSAIRINGKAVPF 284
           FFGS+GPY F PNVDLSKSLIYTPLLFNPVS SIYT+WLPSYEYYI LSAIRIN K VPF
Sbjct: 181 FFGSKGPYKFSPNVDLSKSLIYTPLLFNPVSASIYTYWLPSYEYYIALSAIRINAKTVPF 240

Query: 285 NTSLLAFEPVHGGGGTKISTSTTYALLQTSIYRGFATAFMKEAAALNFTLTNAVEPFGVC 344
           NTSLL FEP+HGGGGTKISTS TYALLQTSIYR FA AF KE+AALNFT T+ V+PFG+C
Sbjct: 241 NTSLLPFEPIHGGGGTKISTSATYALLQTSIYRAFAAAFAKESAALNFTATDPVKPFGLC 300

Query: 345 FAADSVAVTAAGPMAPAVDLVMENGKVVWKLGGRNSMVRIKKNGVDAWCLGFINGGEFPR 404
           +AA+SVA+TAAGP APAVDLVME GK  W+LGGRNSMVR+KK GVDAWCLGFI+G E PR
Sbjct: 301 YAAESVAMTAAGPAAPAVDLVMEGGKSAWRLGGRNSMVRMKKVGVDAWCLGFIDGVENPR 360

Query: 405 TPIVIGGLQMEDHLLQFDLENYRFGFSSSALLKGTSCSNFNFTSAQSFL 451
           TPIVIGGLQMED LLQFDLEN RFGF SS LL+GTSCS FNFTS  +F+
Sbjct: 361 TPIVIGGLQMEDQLLQFDLENSRFGFGSSVLLQGTSCSKFNFTSLDNFI 398

BLAST of Spg014158 vs. TAIR 10
Match: AT1G03220.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 323.2 bits (827), Expect = 3.4e-88
Identity = 189/445 (42.47%), Postives = 270/445 (60.67%), Query Frame = 0

Query: 7   ILFFIFFLLSFPFLSLQTAF-----VAPILKDHNSLLYSISVHLKTPLRPANLHLDLGGA 66
           +L FIF L S    S QT F     + P+ KD ++L Y+  ++ +TPL PA++  DLGG 
Sbjct: 11  LLLFIFSLSS----SAQTPFRPKALLLPVTKDQSTLQYTTVINQRTPLVPASVVFDLGGR 70

Query: 67  FSWIDCYNRYNSSSYRLVKCDSALCNSMDSKVCGSCVEAPAPICANDTIFCDVYPEKPSI 126
             W+DC   Y SS+Y+  +C+SA+C+   S  CG+C   P P C+N+T  C   P     
Sbjct: 71  ELWVDCDKGYVSSTYQSPRCNSAVCSRAGSTSCGTCFSPPRPGCSNNT--CGGIP----- 130

Query: 127 RDQYVDYNHPKLTDSENLITDVLALSTTDGSSSGPLCPISDIPFSCAKTDFLRGLARNVI 186
                D        S     DV+++ +T+GS+ G +  I ++ F C  T  L+GLA+  +
Sbjct: 131 -----DNTVTGTATSGEFALDVVSIQSTNGSNPGRVVKIPNLIFDCGATFLLKGLAKGTV 190

Query: 187 GLAALGRSNESIPSMISAKFNSPRIFAVCLSGTRSGSGVAFFGSRGPYGFFPNVDLSKSL 246
           G+A +GR N  +PS  +A F+  R FAVCL+   SG GVAFFG+ GPY F P + +S SL
Sbjct: 191 GMAGMGRHNIGLPSQFAAAFSFHRKFAVCLT---SGKGVAFFGN-GPYVFLPGIQIS-SL 250

Query: 247 IYTPLLFNPVS-GSIYTWWLPSYEYYIGLSAIRINGKAVPFNTSLLAFEPVHGGGGTKIS 306
             TPLL NPVS  S ++    S EY+IG++AI+I  K VP N +LL      G GGTKIS
Sbjct: 251 QTTPLLINPVSTASAFSQGEKSSEYFIGVTAIQIVEKTVPINPTLLKINASTGIGGTKIS 310

Query: 307 TSTTYALLQTSIYRGFATAFMKEAAALNFTLTNAVEPFGVCFAADSVAVTAAGPMAPAVD 366
           +   Y +L++SIY  F + F+K+AAA +     +V+PFG CF+  +V VT  G   P ++
Sbjct: 311 SVNPYTVLESSIYNAFTSEFVKQAAARSIKRVASVKPFGACFSTKNVGVTRLGYAVPEIE 370

Query: 367 LVMENGKVVWKLGGRNSMVRIKKNGVDAWCLGFINGGEFPRTPIVIGGLQMEDHLLQFDL 426
           LV+ +  VVW++ G NSMV +     D  CLGF++GG   RT +VIGG Q+ED+L++FDL
Sbjct: 371 LVLHSKDVVWRIFGANSMVSVSD---DVICLGFVDGGVNARTSVVIGGFQLEDNLIEFDL 430

Query: 427 ENYRFGFSSSALLKGTSCSNFNFTS 446
            + +FGFSS+ L + T+C+NFNFTS
Sbjct: 431 ASNKFGFSSTLLGRQTNCANFNFTS 431

BLAST of Spg014158 vs. TAIR 10
Match: AT1G03230.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 308.5 bits (789), Expect = 8.7e-84
Identity = 179/447 (40.04%), Postives = 266/447 (59.51%), Query Frame = 0

Query: 7   ILFFIFFLLSFPFLSLQT-------AFVAPILKDHNSLLYSISVHLKTPLRPANLHLDLG 66
           I+ F   LLS   LS          A + P+ KD ++L Y+  ++ +TPL PA++  DLG
Sbjct: 6   IIIFSVLLLSIFSLSSSAQPSFRPKALLLPVTKDPSTLQYTTVINQRTPLVPASVVFDLG 65

Query: 67  GAFSWIDCYNRYNSSSYRLVKCDSALCNSMDSKVCGSCVEAPAPICANDTIFCDVYPEKP 126
           G   W+DC   Y S++YR  +C+SA+C+   S  CG+C   P P C+N+T  C  +P   
Sbjct: 66  GREFWVDCDQGYVSTTYRSPRCNSAVCSRAGSIACGTCFSPPRPGCSNNT--CGAFP--- 125

Query: 127 SIRDQYVDYNHPKLTDSENLITDVLALSTTDGSSSGPLCPISDIPFSCAKTDFLRGLARN 186
                  D +      S     DV+++ +T+GS+ G    I ++ FSC  T  L+GLA+ 
Sbjct: 126 -------DNSITGWATSGEFALDVVSIQSTNGSNPGRFVKIPNLIFSCGSTSLLKGLAKG 185

Query: 187 VIGLAALGRSNESIPSMISAKFNSPRIFAVCLSGTRSGSGVAFFGSRGPYGFFPNVDLSK 246
            +G+A +GR N  +P   +A F+  R FAVCL+   SG GVAFFG+ GPY F P + +S+
Sbjct: 186 AVGMAGMGRHNIGLPLQFAAAFSFNRKFAVCLT---SGRGVAFFGN-GPYVFLPGIQISR 245

Query: 247 SLIYTPLLFNPVSGSI-YTWWLPSYEYYIGLSAIRINGKAVPFNTSLLAFEPVHGGGGTK 306
            L  TPLL NP +    ++    S EY+IG++AI+I  K +P + +LL      G GGTK
Sbjct: 246 -LQKTPLLINPGTTVFEFSKGEKSPEYFIGVTAIKIVEKTLPIDPTLLKINASTGIGGTK 305

Query: 307 ISTSTTYALLQTSIYRGFATAFMKEAAALNFTLTNAVEPFGVCFAADSVAVTAAGPMAPA 366
           IS+   Y +L++SIY+ F + F+++AAA +     +V+PFG CF+  +V VT  G   P 
Sbjct: 306 ISSVNPYTVLESSIYKAFTSEFIRQAAARSIKRVASVKPFGACFSTKNVGVTRLGYAVPE 365

Query: 367 VDLVMENGKVVWKLGGRNSMVRIKKNGVDAWCLGFINGGEFPRTPIVIGGLQMEDHLLQF 426
           + LV+ +  VVW++ G NSMV +     D  CLGF++GG  P   +VIGG Q+ED+L++F
Sbjct: 366 IQLVLHSKDVVWRIFGANSMVSVSD---DVICLGFVDGGVNPGASVVIGGFQLEDNLIEF 425

Query: 427 DLENYRFGFSSSALLKGTSCSNFNFTS 446
           DL + +FGFSS+ L + T+C+NFNFTS
Sbjct: 426 DLASNKFGFSSTLLGRQTNCANFNFTS 432

BLAST of Spg014158 vs. TAIR 10
Match: AT5G19110.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 171.0 bits (432), Expect = 2.2e-42
Identity = 133/446 (29.82%), Postives = 208/446 (46.64%), Query Frame = 0

Query: 5   ASILFFIFFLLSFPFLSLQ--TAFVAPILKDHNSLLYSISVHLKTPLR-PANLHLDLGGA 64
           +S+   + FL  F  ++L+  + ++ PI K   + L+  + ++ +  + P NL LDLG  
Sbjct: 3   SSLTRLLVFLSIFAAIALKSNSQYLLPITKHEPTNLFYTTFNVGSAAKSPVNLLLDLGTN 62

Query: 65  FSWIDCYNRYNSSSYRLVKCDSALCNSMDSKVCG--SCV-EAPAPICANDTIFCDVYPEK 124
            +W+DC    + SS RLV C S+ C S+    C   SC+ + P P+  N  +        
Sbjct: 63  LTWLDCRKLKSLSSLRLVTCQSSTCKSIPGNGCAGKSCLYKQPNPLGQNPVV-------- 122

Query: 125 PSIRDQYVDYNHPKLTDSENLITDVLALSTTDGSSSGPLCPISDIPFSCAKTDFLRGLAR 184
                            +  ++ D  +L TTDG        +    FSCA    L+GL  
Sbjct: 123 -----------------TGRVVQDRASLYTTDGGKFLSQVSVRHFTFSCAGEKALQGLPP 182

Query: 185 NVIGLAALGRSNESIPSMISAKFNSPRIFAVCLSGTRSGSGVAFFGSRGPYGFFPNVDLS 244
            V G+ AL   + S    +++ FN    F++CL      SG   F   G + F P  + S
Sbjct: 183 PVDGVLALSPGSSSFTKQVTSAFNVIPKFSLCL----PSSGTGHFYIAGIHYFIPPFNSS 242

Query: 245 KSLIYTPLLFNPVSGSIYTWWLPSYEYYIGLSAIRINGKAVPFNTSLLAFEPVHGGGGTK 304
            + I  P    P+ G+       S +Y I + +I + G A+  N  LL        GG K
Sbjct: 243 DNPI--PRTLTPIKGT------DSGDYLITVKSIYVGGTALKLNPDLLT-------GGAK 302

Query: 305 ISTSTTYALLQTSIYRGFATAFMKEAAALNFTLTNAVEPFGVCFAADSVAVT-AAGPMAP 364
           +ST   Y +LQT IY   A +F  +A A+      +V PF  CF + +      AGP  P
Sbjct: 303 LSTVVHYTVLQTDIYNALAQSFTLKAKAMGIAKVPSVAPFKHCFDSRTAGKNLTAGPNVP 362

Query: 365 AVDLVMEN--GKVVWKLGGRNSMVRIKKNGVDAWCLGFINGGEFPRTPIVIGGLQMEDHL 424
            +++ +    G+V W   G N++V++K+      CL FI+GG+ P+  +VIG  Q++DH+
Sbjct: 363 VIEIGLPGRIGEVKWGFYGANTVVKVKET---VMCLAFIDGGKTPKDLMVIGTHQLQDHM 401

Query: 425 LQFDLENYRFGFSSSALLKGTSCSNF 442
           L+FD       FS S LL  TSCS +
Sbjct: 423 LEFDFSGTVLAFSESLLLHNTSCSTW 401

BLAST of Spg014158 vs. TAIR 10
Match: AT5G19100.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 168.3 bits (425), Expect = 1.4e-41
Identity = 146/446 (32.74%), Postives = 206/446 (46.19%), Query Frame = 0

Query: 11  IFFLLSFPFLSLQT---------AFVAPILKDHNSLLYSISVHLKTPLRPANLHLDLGGA 70
           IF LLS  FL L           +F+ PI KD    +Y+I + + +        LDL GA
Sbjct: 6   IFLLLSLVFLYLANTSHSLRKFQSFLHPIYKDTAKNIYTIPLSIGS-TSSEKFVLDLNGA 65

Query: 71  FSWI-DCYNRYNSSSYRLVKCDSALCNSMDSKVCGSCVEAPAPICANDTIFCDVYPEKPS 130
              + +C     S++Y  ++C S  C     K        P  + A     C        
Sbjct: 66  APLLQNCPTAAKSTTYHPIRCGSTRC-----KYANPNFPCPNNVIAKKRTVCLSSDNSRL 125

Query: 131 IRDQ---YVDYNHPKLTDSENLITDVLALSTTDGSSSGPLCPISDIPFSCAKTDFLRGLA 190
            RD       +N     DSE  ++  L L+ TDG+ +                     L 
Sbjct: 126 FRDTVPLLYTFNGVYTRDSE--MSSSLTLTCTDGAPA---------------------LK 185

Query: 191 RNVIGLAALGRSNESIPSMISAKFNSPRIFAVCLSGT---RSGSGVAFFGSRGPYGFFP- 250
           +  IGLA    ++ SIPS + + +  P   A+CL  T   +S +G  + G +G Y + P 
Sbjct: 186 QRTIGLA---NTHLSIPSQLISMYQLPHKIALCLPSTERSQSHNGDLWIG-KGEYYYLPY 245

Query: 251 NVDLSKSLIYTPLLFNPVSGSIYTWWLPSYEYYIGLSAIRINGKAVPFNTSLLAFEPVHG 310
           + D+SK    TPL+ N  SG          EY I + +I+I  K VP             
Sbjct: 246 DKDVSKIFASTPLIGNGKSG----------EYLIDVKSIQIGAKTVPIPY---------- 305

Query: 311 GGGTKISTSTTYALLQTSIYRGFATAFMKEAAALNFTLTNAVEPFGVCFAADSVAVTAAG 370
            G TKIST   Y + QTS+Y+   TAF +    +      AV+PFG CF ++       G
Sbjct: 306 -GATKISTLAPYTVFQTSLYKALLTAFTEN---IKIAKAPAVKPFGACFYSN------GG 365

Query: 371 PMAPAVDLVMENGKVVWKLGGRNSMVRIKKNGVDAWCLGFINGGEFPRTPIVIGGLQMED 430
              P +DLV+  G   W++ G NS+V++ KN V   CLGF++GG  P+ PIVIGG QMED
Sbjct: 366 RGVPVIDLVLSGG-AKWRIYGSNSLVKVNKNVV---CLGFVDGGVKPKYPIVIGGFQMED 384

Query: 431 HLLQFDLENYRFGFSSSALLKGTSCS 440
           +L++FDLE  +F FSSS LL  TSCS
Sbjct: 426 NLVEFDLEASKFSFSSSLLLHNTSCS 384

BLAST of Spg014158 vs. TAIR 10
Match: AT5G19120.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 154.5 bits (389), Expect = 2.1e-37
Identity = 139/439 (31.66%), Postives = 202/439 (46.01%), Query Frame = 0

Query: 8   LFFIFFLLSFPFLSLQ-----TAFVAPILKDHNSLLYSISVHLKTPLRPANLHLDLGGAF 67
           LFF  FL +      Q        V P++KD  +  Y   + L     P  L +DL G+ 
Sbjct: 9   LFFFSFLSALIISKSQISDSVNGVVFPVVKDLPTGQYLAQIRLGDSPDPVKLVVDLAGSI 68

Query: 68  SWIDCYNRYNSSSYRLVKCDSALCNSMDSKVCGSCVEAPAPICANDTIFCDVYPEKPSIR 127
            W DC +R+ SSS  L+   S+ C  + +KV    V + +    +    C++  +  +  
Sbjct: 69  LWFDCSSRHVSSSRNLISGSSSGC--LKAKVGNERVSSSSSSRKDQNADCELLVKNDAF- 128

Query: 128 DQYVDYNHPKLTDSENLITDVLALSTTDGSSSGPLCPISDIPFSCAKTDFLRGLARNVIG 187
                     +T    L +DV+++    GS + P     D+ F+C     LRGLA    G
Sbjct: 129 ---------GITARGELFSDVMSV----GSVTSP--GTVDLLFACTPPWLLRGLASGAQG 188

Query: 188 LAALGRSNESIPSMISAKFNSPRIFAVCLS---GTRSGSGV-AFFGSRGPYGFFPNVDLS 247
           +  LGR+  S+PS ++A+ N  R   V LS   G  S S V   FG          V  S
Sbjct: 189 VMGLGRAQISLPSQLAAETNERRRLTVYLSPLNGVVSTSSVEEVFG----------VAAS 248

Query: 248 KSLIYTPLLFNPVSGSIYTWWLPSYEYYIGLSAIRINGKAVPFNTSLLAFEPVHGGGGTK 307
           +SL+YTPLL    +GS       S  Y I + +IR+NG+ +           V G    +
Sbjct: 249 RSLVYTPLL----TGS-------SGNYVINVKSIRVNGEKL----------SVEGPLAVE 308

Query: 308 ISTSTTYALLQTSIYRGFATAFMKEAAALNFTLTNAVEPFGVCFAADSVAVTAAGPMAPA 367
           +ST   Y +L++SIY+ FA A+ K  AA   T    V PFG+CF +D           PA
Sbjct: 309 LSTVVPYTILESSIYKVFAEAYAK--AAGEATSVPPVAPFGLCFTSD--------VDFPA 368

Query: 368 VDLVMENGKVVWKLGGRNSMVRIKKNGVDAWCLGFINGGEFPRTPIVIGGLQMEDHLLQF 427
           VDL +++  V W++ G+N MV +   G    C G ++GG     PIV+GGLQ+E  +L F
Sbjct: 369 VDLALQSEMVRWRIHGKNLMVDV---GGGVRCSGIVDGGSSRVNPIVMGGLQLEGFILDF 385

Query: 428 DLENYRFGFSSSALLKGTS 438
           DL N   GF        TS
Sbjct: 429 DLGNSMMGFGQRTRSDSTS 385

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022959602.11.7e-20681.56basic 7S globulin 2-like [Cucurbita moschata][more]
XP_038897313.11.4e-20582.88probable aspartic proteinase GIP1 [Benincasa hispida][more]
KAG7027263.17.1e-20580.67hypothetical protein SDJN02_11275, partial [Cucurbita argyrosperma subsp. argyro... [more]
XP_023004536.11.2e-20480.44basic 7S globulin 2-like [Cucurbita maxima][more]
XP_023514746.11.6e-20480.44basic 7S globulin 2-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
I1JNS65.6e-9646.06Probable aspartic proteinase GIP1 OS=Glycine max OX=3847 GN=GIP1 PE=1 SV=2[more]
P0DO212.8e-8743.60Probable aspartic proteinase GIP2 OS=Nicotiana benthamiana OX=4100 GN=GIP2 PE=1 ... [more]
P829522.8e-6335.29Gamma conglutin 1 OS=Prunus dulcis OX=3755 GN=Cgamma1 PE=1 SV=2[more]
Q8RVH51.8e-4632.20Basic 7S globulin 2 OS=Glycine max OX=3847 PE=1 SV=1[more]
P139175.3e-4632.40Basic 7S globulin OS=Glycine max OX=3847 GN=BG PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1H5018.2e-20781.56basic 7S globulin 2-like OS=Cucurbita moschata OX=3662 GN=LOC111460627 PE=4 SV=1[more]
A0A6J1KSD25.9e-20580.44basic 7S globulin 2-like OS=Cucurbita maxima OX=3661 GN=LOC111497811 PE=4 SV=1[more]
A0A5D3D4L47.7e-19777.73Basic 7S globulin 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold2032... [more]
A0A0A0K5069.4e-19576.52Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G39005... [more]
A0A6J1D7R81.3e-16774.33LOW QUALITY PROTEIN: basic 7S globulin 2 OS=Momordica charantia OX=3673 GN=LOC11... [more]
Match NameE-valueIdentityDescription
AT1G03220.13.4e-8842.47Eukaryotic aspartyl protease family protein [more]
AT1G03230.18.7e-8440.04Eukaryotic aspartyl protease family protein [more]
AT5G19110.12.2e-4229.82Eukaryotic aspartyl protease family protein [more]
AT5G19100.11.4e-4132.74Eukaryotic aspartyl protease family protein [more]
AT5G19120.12.1e-3731.66Eukaryotic aspartyl protease family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 227..445
e-value: 1.0E-59
score: 203.4
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 20..225
e-value: 5.2E-42
score: 146.1
IPR021109Aspartic peptidase domain superfamilySUPERFAMILY50630Acid proteasescoord: 30..436
IPR032861Xylanase inhibitor, N-terminalPFAMPF14543TAXi_Ncoord: 39..225
e-value: 4.3E-27
score: 95.5
IPR032799Xylanase inhibitor, C-terminalPFAMPF14541TAXi_Ccoord: 264..428
e-value: 5.0E-47
score: 159.7
IPR001461Aspartic peptidase A1 familyPANTHERPTHR47965ASPARTYL PROTEASE-RELATEDcoord: 8..443
NoneNo IPR availablePANTHERPTHR47965:SF6OS05G0403000 PROTEINcoord: 8..443
IPR033121Peptidase family A1 domainPROSITEPS51767PEPTIDASE_A1coord: 39..428
score: 18.427147

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg014158.1Spg014158.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
molecular_function GO:0004190 aspartic-type endopeptidase activity