Homology
BLAST of Spg013912 vs. NCBI nr
Match:
KAG6575469.1 (Beta-fructofuranosidase, insoluble isoenzyme CWINV1, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 1040.4 bits (2689), Expect = 5.7e-300
Identity = 494/570 (86.67%), Postives = 526/570 (92.28%), Query Frame = 0
Query: 1 MASLSLWFISFLGLLLGHGVLQLQASHHVYKAQTSHFVPHDYQQPYRTSYHFQPPKNWMN 60
MAS SL FISFL LL HG L+LQASHHVY+AQ S + H Y+QPYRTSYHFQPPKNW+N
Sbjct: 1 MASSSLCFISFLCFLLCHGALKLQASHHVYRAQASRLLLHRYEQPYRTSYHFQPPKNWIN 60
Query: 61 GPMIFKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWVPHEYAIYPSQPSDINGCWSGS 120
GPMI+KGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINW PH+YAIYPSQPSDINGCWSGS
Sbjct: 61 GPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWKPHDYAIYPSQPSDINGCWSGS 120
Query: 121 ATILPGAKPAILYTGINPKSQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPENHINSS 180
ATILPG KPAILYTGINPK+QQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTP+N INSS
Sbjct: 121 ATILPGDKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQNQINSS 180
Query: 181 SFRDPTTAWLGPDRQWRVIIGSKVDARGLAIMYRSKDFVQWDQVDHPLHYADGMGMWECP 240
SFRDPTTAWLGPD +WRVIIGSK+DARGLAIM++SKDFV+WDQVDHPLHYADG GMWECP
Sbjct: 181 SFRDPTTAWLGPDSEWRVIIGSKIDARGLAIMFQSKDFVKWDQVDHPLHYADGTGMWECP 240
Query: 241 DFFPVVKKGRTGIDTVVNGPHVKHVLKISLDDTKHDLYTIGTYKLDKDIYVPDKGSIENY 300
DFFPV K GR G+DT V+G HVKHVLK+SLDDTKHD YT+GTY L KDIYVPDKGSIE Y
Sbjct: 241 DFFPVAKNGRAGVDTSVSGRHVKHVLKVSLDDTKHDHYTVGTYDLKKDIYVPDKGSIEGY 300
Query: 301 NGLRYDYGKYYASKTFFDSQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRTVWLDASG 360
GLRYDYGKYYASKTFFDSQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPR VWLDASG
Sbjct: 301 TGLRYDYGKYYASKTFFDSQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRAVWLDASG 360
Query: 361 KQLIQWPIVEIHKLRKDKVKLTNKVLKKGSVVEVKGVTAVQADVEVSFKVKNFNKAEVLK 420
KQLIQWPIVEI KLRKDKVKLTNKVLKKGS++ VKGVTA QADV+VSFKVK+FNKA+ LK
Sbjct: 361 KQLIQWPIVEIQKLRKDKVKLTNKVLKKGSIIAVKGVTAAQADVDVSFKVKSFNKAQPLK 420
Query: 421 PEWKDPQLLCSQKGTAADGGVGPFGLLVLASKDLKEYTSVLFRIFKHYNKYVVLLCSDQS 480
EWKDPQL+CSQ G AA GGVGPFGLLVLAS +L+EYTSV FRIFK +KYVVL+CSDQS
Sbjct: 421 AEWKDPQLVCSQMGAAAKGGVGPFGLLVLASNNLEEYTSVFFRIFKRQDKYVVLMCSDQS 480
Query: 481 RSSLNSNNDKTTYGAFLDVDPVSENLSLRSLIDHSIVESFGSKGKACITARVYPTLAIGD 540
RSSLN+NNDKTTYGAFLDVDPV EN+SLRSLIDHSIVESFGSKGKACITARVYP LAIGD
Sbjct: 481 RSSLNANNDKTTYGAFLDVDPVRENISLRSLIDHSIVESFGSKGKACITARVYPRLAIGD 540
Query: 541 KVGLYAFNNGTEPVTITKLNAWSLNKAQIS 571
K GLYAFNNGTE VTITKL+AWS+ KAQIS
Sbjct: 541 KAGLYAFNNGTESVTITKLSAWSMKKAQIS 570
BLAST of Spg013912 vs. NCBI nr
Match:
XP_038898203.1 (beta-fructofuranosidase, insoluble isoenzyme CWINV1-like [Benincasa hispida])
HSP 1 Score: 1039.3 bits (2686), Expect = 1.3e-299
Identity = 492/573 (85.86%), Postives = 527/573 (91.97%), Query Frame = 0
Query: 1 MASLSLWFISFLGLLLGHGVLQLQASHHVYKAQTSHFVPHDYQQPYRTSYHFQPPKNWM- 60
MAS SL FISFL L GHGVLQLQASHHVY+AQ S+ +PH YQQPYRTSYHFQPPKNW+
Sbjct: 1 MASFSLCFISFLCFLFGHGVLQLQASHHVYRAQLSNSLPHHYQQPYRTSYHFQPPKNWIN 60
Query: 61 --NGPMIFKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWVPHEYAIYPSQPSDINGCW 120
NGPMI+KGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINW PH++AIYPSQPSDINGCW
Sbjct: 61 DPNGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWEPHDHAIYPSQPSDINGCW 120
Query: 121 SGSATILPGAKPAILYTGINPKSQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPENHI 180
SGSATILPG KPAILYTGINPK+QQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTP+NHI
Sbjct: 121 SGSATILPGDKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQNHI 180
Query: 181 NSSSFRDPTTAWLGPDRQWRVIIGSKVDARGLAIMYRSKDFVQWDQVDHPLHYADGMGMW 240
NSSSFRDPTTAWLGPD +WRVIIGSK++ARGLAIMY+SKDFV+WDQVDHPLHYADG GMW
Sbjct: 181 NSSSFRDPTTAWLGPDGEWRVIIGSKINARGLAIMYQSKDFVKWDQVDHPLHYADGTGMW 240
Query: 241 ECPDFFPVVKKGRTGIDTVVNGPHVKHVLKISLDDTKHDLYTIGTYKLDKDIYVPDKGSI 300
ECPDFFPV K GR G+DT V+G HVKHVLK+SLDDTKHD YT+G Y L+KDIYVP+KGSI
Sbjct: 241 ECPDFFPVAKTGRKGVDTSVSGGHVKHVLKVSLDDTKHDHYTVGNYDLEKDIYVPNKGSI 300
Query: 301 ENYNGLRYDYGKYYASKTFFDSQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRTVWLD 360
E Y GLRYDYGKYYASKTF+D QKKRRVLWGWVNESSSV+DDIKKGWSG+QAIPRT+WLD
Sbjct: 301 EGYMGLRYDYGKYYASKTFYDGQKKRRVLWGWVNESSSVEDDIKKGWSGIQAIPRTIWLD 360
Query: 361 ASGKQLIQWPIVEIHKLRKDKVKLTNKVLKKGSVVEVKGVTAVQADVEVSFKVKNFNKAE 420
ASGKQLIQWPI EI KLRKDKVKLTNKVLKKGS++EVKGVTA QADVEVSFKVKNF KA+
Sbjct: 361 ASGKQLIQWPIEEIQKLRKDKVKLTNKVLKKGSLIEVKGVTAAQADVEVSFKVKNFKKAQ 420
Query: 421 VLKPEWKDPQLLCSQKGTAADGGVGPFGLLVLASKDLKEYTSVLFRIFKHYNKYVVLLCS 480
+LKPEWKDPQLLCSQKG GGVG FGLLVLASKDLKEYTS+ F IFK KY+VL+CS
Sbjct: 421 ILKPEWKDPQLLCSQKGATTKGGVGSFGLLVLASKDLKEYTSISFTIFKRQGKYIVLMCS 480
Query: 481 DQSRSSLNSNNDKTTYGAFLDVDPVSENLSLRSLIDHSIVESFGSKGKACITARVYPTLA 540
DQSRSSLN NNDKTTYGAFL+VDPVSE+LSLRSLIDHS+VESFGSKGKACITARVYPTLA
Sbjct: 481 DQSRSSLNPNNDKTTYGAFLNVDPVSEDLSLRSLIDHSVVESFGSKGKACITARVYPTLA 540
Query: 541 IGDKVGLYAFNNGTEPVTITKLNAWSLNKAQIS 571
IGD VGLY FNNGTE VTITKL AWSL KAQI+
Sbjct: 541 IGDNVGLYVFNNGTESVTITKLGAWSLKKAQIN 573
BLAST of Spg013912 vs. NCBI nr
Match:
XP_023547447.1 (beta-fructofuranosidase, insoluble isoenzyme CWINV1-like [Cucurbita pepo subsp. pepo])
HSP 1 Score: 1028.1 bits (2657), Expect = 2.9e-296
Identity = 491/572 (85.84%), Postives = 521/572 (91.08%), Query Frame = 0
Query: 1 MASLSLWFISFLGLLLGHGVLQLQASHHVYKAQTSHFVPHDYQQPYRTSYHFQPPKNWM- 60
MAS SL FISFL LL HG L+LQASHHVY AQ S + H ++QPYRTSYHFQPPKNW+
Sbjct: 1 MASSSLCFISFLCFLLAHGALKLQASHHVYTAQASRLLLHHHEQPYRTSYHFQPPKNWIN 60
Query: 61 --NGPMIFKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWVPHEYAIYPSQPSDINGCW 120
NGPMI+KGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINW PH+YAIYPSQPSDINGCW
Sbjct: 61 DPNGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWKPHDYAIYPSQPSDINGCW 120
Query: 121 SGSATILPGAKPAILYTGINPKSQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPENHI 180
SGSATILPG KPAILYTGINPK+QQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTP+NHI
Sbjct: 121 SGSATILPGDKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQNHI 180
Query: 181 NSSSFRDPTTAWLGPDRQWRVIIGSKVDARGLAIMYRSKDFVQWDQVDHPLHYADGMGMW 240
NSSSFRDPTTAWLG D +WRVIIGSK+DARGLAIM+RSKDFV+WDQVDHPLHYADGMGMW
Sbjct: 181 NSSSFRDPTTAWLGHDGEWRVIIGSKIDARGLAIMFRSKDFVKWDQVDHPLHYADGMGMW 240
Query: 241 ECPDFFPVVKKGRTGIDTVVNGPHVKHVLKISLDDTKHDLYTIGTYKLDKDIYVPDKGSI 300
ECPDFFPV K G G+DT V+G HVKHVLK+SLDDTKHD YT+GTY L KDIYVPDKGSI
Sbjct: 241 ECPDFFPVAKNGHAGVDTSVSGQHVKHVLKVSLDDTKHDQYTVGTYDLKKDIYVPDKGSI 300
Query: 301 ENYNGLRYDYGKYYASKTFFDSQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRTVWLD 360
E+Y GLRYDYGKYYASKTFFD QKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPR VWLD
Sbjct: 301 EDYTGLRYDYGKYYASKTFFDGQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRAVWLD 360
Query: 361 ASGKQLIQWPIVEIHKLRKDKVKLTNKVLKKGSVVEVKGVTAVQADVEVSFKVKNFNKAE 420
ASGKQLIQWPI EI KLRKDK+KLTNKVLKKGS+V VKGVTA QADV+VSFKVK+FNKA+
Sbjct: 361 ASGKQLIQWPIAEIQKLRKDKIKLTNKVLKKGSIVAVKGVTAAQADVDVSFKVKSFNKAQ 420
Query: 421 VLKPEWKDPQLLCSQKGTAADGGVGPFGLLVLASKDLKEYTSVLFRIFKHYNKYVVLLCS 480
LK EWKDPQL+CSQ G AA GGVGPFGLLVLAS +L+EYTSV FRIFK +KYVVL+CS
Sbjct: 421 RLKAEWKDPQLVCSQMGAAAKGGVGPFGLLVLASNNLEEYTSVFFRIFKGQDKYVVLMCS 480
Query: 481 DQSRSSLNSNNDKTTYGAFLDVDPVSENLSLRSLIDHSIVESFGSKGKACITARVYPTLA 540
DQSRSSLN+NNDKTTYGAFLDVDPV EN+SLRSLIDHSIVESFGSKGKACITARVYP LA
Sbjct: 481 DQSRSSLNANNDKTTYGAFLDVDPVRENVSLRSLIDHSIVESFGSKGKACITARVYPRLA 540
Query: 541 IGDKVGLYAFNNGTEPVTITKLNAWSLNKAQI 570
IGDK GLYAFNNGTE VTI KL AWSL KAQI
Sbjct: 541 IGDKAGLYAFNNGTESVTIAKLRAWSLKKAQI 572
BLAST of Spg013912 vs. NCBI nr
Match:
XP_022954011.1 (beta-fructofuranosidase, insoluble isoenzyme CWINV1-like [Cucurbita moschata] >XP_022954012.1 beta-fructofuranosidase, insoluble isoenzyme CWINV1-like [Cucurbita moschata])
HSP 1 Score: 1027.7 bits (2656), Expect = 3.8e-296
Identity = 491/573 (85.69%), Postives = 522/573 (91.10%), Query Frame = 0
Query: 1 MASLSLWFISFLGLLLGHGVLQLQASHHVYKAQTSHFVPHDYQQPYRTSYHFQPPKNWM- 60
MAS SL FISFL LL HG L+LQASHHVY Q S + H Y+QPYRTSYHFQPPKNW+
Sbjct: 1 MASSSLCFISFLCFLLCHGALKLQASHHVYTPQPSRLLLHRYEQPYRTSYHFQPPKNWIN 60
Query: 61 --NGPMIFKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWVPHEYAIYPSQPSDINGCW 120
NGPMI+KGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINW PH+YAIYPSQPSDINGCW
Sbjct: 61 DPNGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWKPHDYAIYPSQPSDINGCW 120
Query: 121 SGSATILPGAKPAILYTGINPKSQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPENHI 180
SGSATILPG KPAILYTGINPK+QQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTP+NHI
Sbjct: 121 SGSATILPGDKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQNHI 180
Query: 181 NSSSFRDPTTAWLGPDRQWRVIIGSKVDARGLAIMYRSKDFVQWDQVDHPLHYADGMGMW 240
NSSSFRDPTTAWLG D +WRVIIGSK+DARGLAIM++SKDFV+WDQVDHPLHYADG GMW
Sbjct: 181 NSSSFRDPTTAWLGHDGEWRVIIGSKIDARGLAIMFQSKDFVKWDQVDHPLHYADGTGMW 240
Query: 241 ECPDFFPVVKKGRTGIDTVVNGPHVKHVLKISLDDTKHDLYTIGTYKLDKDIYVPDKGSI 300
ECPDFFPV K R G+DT V+G HVKHVLK+SLDDTKHD YT+GTY L KDIYVPDKGSI
Sbjct: 241 ECPDFFPVAKNSRAGVDTSVSGQHVKHVLKVSLDDTKHDQYTVGTYDLKKDIYVPDKGSI 300
Query: 301 ENYNGLRYDYGKYYASKTFFDSQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRTVWLD 360
E Y GLRYDYGKYYASKTFFD QKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPR VWLD
Sbjct: 301 EGYTGLRYDYGKYYASKTFFDGQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRAVWLD 360
Query: 361 ASGKQLIQWPIVEIHKLRKDKVKLTNKVLKKGSVVEVKGVTAVQADVEVSFKVKNFNKAE 420
ASGKQLIQWPIVEI KLRKDKVKLTNKVLKKGS++ VKGVTA QADV+VSFKVK+FNKA+
Sbjct: 361 ASGKQLIQWPIVEIQKLRKDKVKLTNKVLKKGSIIAVKGVTAAQADVDVSFKVKSFNKAQ 420
Query: 421 VLKPEWKDPQLLCSQKGTAADGGVGPFGLLVLASKDLKEYTSVLFRIFKHYNKYVVLLCS 480
LK EWKDPQL+CSQ G AA GGVGPFGLLVLAS +L+EYTSV FRIFK +KYVVL+CS
Sbjct: 421 PLKAEWKDPQLVCSQMGAAAKGGVGPFGLLVLASNNLEEYTSVFFRIFKRQDKYVVLMCS 480
Query: 481 DQSRSSLNSNNDKTTYGAFLDVDPVSENLSLRSLIDHSIVESFGSKGKACITARVYPTLA 540
DQSRSSLN+NNDKTTYGAFLDVDPV EN+SLRSLIDHSIVESFGSKGKACITARVYP LA
Sbjct: 481 DQSRSSLNANNDKTTYGAFLDVDPVRENISLRSLIDHSIVESFGSKGKACITARVYPRLA 540
Query: 541 IGDKVGLYAFNNGTEPVTITKLNAWSLNKAQIS 571
IGDK GLYAFNNGTE VTITKL+AWS+ KAQIS
Sbjct: 541 IGDKAGLYAFNNGTESVTITKLSAWSMKKAQIS 573
BLAST of Spg013912 vs. NCBI nr
Match:
KAG6575468.1 (Beta-fructofuranosidase, insoluble isoenzyme CWINV1, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 1027.3 bits (2655), Expect = 5.0e-296
Identity = 488/569 (85.76%), Postives = 521/569 (91.56%), Query Frame = 0
Query: 1 MASLSLWFISFLGLLLGHGVLQLQASHHVYKAQTSHFVPHDYQQPYRTSYHFQPPKNWMN 60
MAS SL FISFL LL HG L+LQASHHVY Q S + H ++QPYRTS HFQPPKNW+N
Sbjct: 1 MASSSLCFISFLCFLLCHGALKLQASHHVYTPQPSRLLLHHHEQPYRTSCHFQPPKNWIN 60
Query: 61 GPMIFKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWVPHEYAIYPSQPSDINGCWSGS 120
GPMI+KGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINW PH+YAIYPSQPSDINGCWSGS
Sbjct: 61 GPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWKPHDYAIYPSQPSDINGCWSGS 120
Query: 121 ATILPGAKPAILYTGINPKSQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPENHINSS 180
ATILPG KPAILYTGINPK+QQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTP+N INSS
Sbjct: 121 ATILPGDKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQNQINSS 180
Query: 181 SFRDPTTAWLGPDRQWRVIIGSKVDARGLAIMYRSKDFVQWDQVDHPLHYADGMGMWECP 240
SFRDPTTAWLGPD +WRVIIGSK+DARGLAIM+RSKDFV+WDQVDHPLHYADGMGMWECP
Sbjct: 181 SFRDPTTAWLGPDGEWRVIIGSKIDARGLAIMFRSKDFVKWDQVDHPLHYADGMGMWECP 240
Query: 241 DFFPVVKKGRTGIDTVVNGPHVKHVLKISLDDTKHDLYTIGTYKLDKDIYVPDKGSIENY 300
DFFPV K GR G+DT V+G HVKHVLK+SLDDTKHD YT+GTY L KDIYVPDKGSIE+Y
Sbjct: 241 DFFPVAKNGRAGVDTSVSGQHVKHVLKVSLDDTKHDQYTVGTYDLKKDIYVPDKGSIEDY 300
Query: 301 NGLRYDYGKYYASKTFFDSQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRTVWLDASG 360
GLRYDYGKYYASKTFFD QKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRTVWLDASG
Sbjct: 301 RGLRYDYGKYYASKTFFDDQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRTVWLDASG 360
Query: 361 KQLIQWPIVEIHKLRKDKVKLTNKVLKKGSVVEVKGVTAVQADVEVSFKVKNFNKAEVLK 420
KQLIQWPI+EI KLR DKVKL +KVLKKGS++ VKGVTA QADV+VSFKVK+FNKA+ LK
Sbjct: 361 KQLIQWPIMEIQKLRNDKVKLADKVLKKGSIIAVKGVTAAQADVDVSFKVKSFNKAQKLK 420
Query: 421 PEWKDPQLLCSQKGTAADGGVGPFGLLVLASKDLKEYTSVLFRIFKHYNKYVVLLCSDQS 480
EWKDPQL+CSQ G AA GGVGPFGLLVLAS +L+EYTSV FRIFK +KYVVL+CSDQS
Sbjct: 421 AEWKDPQLVCSQMGAAAKGGVGPFGLLVLASNNLEEYTSVFFRIFKRQDKYVVLMCSDQS 480
Query: 481 RSSLNSNNDKTTYGAFLDVDPVSENLSLRSLIDHSIVESFGSKGKACITARVYPTLAIGD 540
RSSLN+NNDKTTYGAFLDVDPV +N+SLRSLIDHSIVESFGSKGKACITARVYP LAIGD
Sbjct: 481 RSSLNANNDKTTYGAFLDVDPVRKNISLRSLIDHSIVESFGSKGKACITARVYPRLAIGD 540
Query: 541 KVGLYAFNNGTEPVTITKLNAWSLNKAQI 570
K GLYAFNNGTE VTITKL AWSL KAQI
Sbjct: 541 KAGLYAFNNGTESVTITKLRAWSLKKAQI 569
BLAST of Spg013912 vs. ExPASy Swiss-Prot
Match:
Q43866 (Beta-fructofuranosidase, insoluble isoenzyme CWINV1 OS=Arabidopsis thaliana OX=3702 GN=CWINV1 PE=1 SV=1)
HSP 1 Score: 800.4 bits (2066), Expect = 1.3e-230
Identity = 391/581 (67.30%), Postives = 462/581 (79.52%), Query Frame = 0
Query: 2 ASLSLWFISFLGLLLGHGVLQLQASHHVYK--AQTSHFVPHDYQQPYRTSYHFQPPKNWM 61
+++ LW + L LL+G+ V+ L+ASHHVYK Q+++ QPYRT +HFQPPKNWM
Sbjct: 7 SNIGLWLL--LTLLIGNYVVNLEASHHVYKRLTQSTNTKSPSVNQPYRTGFHFQPPKNWM 66
Query: 62 ---NGPMIFKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWVPHEYAIYPSQPSDINGC 121
NGPMI+KGIYHLFYQ+NPKGAVWGNIVWAHSTSTDLINW PH AI+PS P DINGC
Sbjct: 67 NDPNGPMIYKGIYHLFYQWNPKGAVWGNIVWAHSTSTDLINWDPHPPAIFPSAPFDINGC 126
Query: 122 WSGSATILPGAKPAILYTGINPKSQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPENH 181
WSGSATILP KP ILYTGI+PK+QQVQN+A PKNLSDPYLREW KSP NPLMAP N
Sbjct: 127 WSGSATILPNGKPVILYTGIDPKNQQVQNIAEPKNLSDPYLREWKKSPLNPLMAPDAVNG 186
Query: 182 INSSSFRDPTTAWLGPDRQWRVIIGSKVDARGLAIMYRSKDFVQWDQVDHPLHYADGMGM 241
IN+SSFRDPTTAWLG D++WRVIIGSK+ RGLAI Y SKDF++W++ PLHY DG GM
Sbjct: 187 INASSFRDPTTAWLGQDKKWRVIIGSKIHRRGLAITYTSKDFLKWEKSPEPLHYDDGSGM 246
Query: 242 WECPDFFPVVKKGRTGIDTVVNG-PH--VKHVLKISLDDTKHDLYTIGTYKLDKDIYVPD 301
WECPDFFPV + G G++T G P+ +KHVLKISLDDTKHD YTIGTY KD +VPD
Sbjct: 247 WECPDFFPVTRFGSNGVETSSFGEPNEILKHVLKISLDDTKHDYYTIGTYDRVKDKFVPD 306
Query: 302 KGSIENYNGLRYDYGKYYASKTFFDSQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRT 361
G + RYDYGKYYASKTFFDS K RR+LWGW NESSSV+DD++KGWSG+Q IPR
Sbjct: 307 NGFKMDGTAPRYDYGKYYASKTFFDSAKNRRILWGWTNESSSVEDDVEKGWSGIQTIPRK 366
Query: 362 VWLDASGKQLIQWPIVEIHKLRKDKVK-LTNKVLKKGSVVEVKGVTAVQADVEVSFKVKN 421
+WLD SGKQLIQWP+ E+ +LR +VK L NKVLK GS +EV GVTA QADVEV FKV++
Sbjct: 367 IWLDRSGKQLIQWPVREVERLRTKQVKNLRNKVLKSGSRLEVYGVTAAQADVEVLFKVRD 426
Query: 422 FNKAEVLKPEWKDPQLLCSQKGTAADGGVGPFGLLVLASKDLKEYTSVLFRIFK---HYN 481
KA+V++P W DPQL+CS+ + G+GPFGL+VLASK+L+EYTSV FRIFK + N
Sbjct: 427 LEKADVIEPSWTDPQLICSKMNVSVKSGLGPFGLMVLASKNLEEYTSVYFRIFKARQNSN 486
Query: 482 KYVVLLCSDQSRSSLNSNNDKTTYGAFLDVDPVSENLSLRSLIDHSIVESFGSKGKACIT 541
KYVVL+CSDQSRSSL +NDKTTYGAF+D++P + LSLR+LIDHS+VESFG KG+ACIT
Sbjct: 487 KYVVLMCSDQSRSSLKEDNDKTTYGAFVDINP-HQPLSLRALIDHSVVESFGGKGRACIT 546
Query: 542 ARVYPTLAIGDKVGLYAFNNGTEPVTITKLNAWSLNKAQIS 571
+RVYP LAIG L+AFN G + V + LNAWS+N AQIS
Sbjct: 547 SRVYPKLAIGKSSHLFAFNYGYQSVDVLNLNAWSMNSAQIS 584
BLAST of Spg013912 vs. ExPASy Swiss-Prot
Match:
Q43089 (Beta-fructofuranosidase, cell wall isozyme OS=Pisum sativum OX=3888 GN=BFRUCT1 PE=2 SV=1)
HSP 1 Score: 741.5 bits (1913), Expect = 7.1e-213
Identity = 355/553 (64.20%), Postives = 430/553 (77.76%), Query Frame = 0
Query: 1 MASLSLWFISFLGLLLGHGVLQLQASHHVY---KAQTSHFVPHDYQQPYRTSYHFQPPKN 60
MA S++ +S L+ V+ ++A+HHVY + +SH QPYRT+YHFQP KN
Sbjct: 1 MAISSIFLLSLFSLIY---VIPIEATHHVYQTLETLSSHHSSKSNHQPYRTAYHFQPLKN 60
Query: 61 WM---NGPMIFKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWVPHEYAIYPSQPSDIN 120
W+ NGPM + G YHLFYQYNPKGAVWGNIVWAHS S DL+NW P ++AI+PSQPSDI
Sbjct: 61 WINDPNGPMRYGGFYHLFYQYNPKGAVWGNIVWAHSVSKDLVNWTPLDHAIHPSQPSDIK 120
Query: 121 GCWSGSATILPGAKPAILYTGINPKSQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPE 180
GCWSGSATILPG KPAILYTGI+P + QVQN+A+PKN+SDP LREW KSPKNPLM PT
Sbjct: 121 GCWSGSATILPGGKPAILYTGIDPNNHQVQNIAIPKNMSDPLLREWKKSPKNPLMEPTIA 180
Query: 181 NHINSSSFRDPTTAWLGPDRQWRVIIGSKVDARGLAIMYRSKDFVQWDQVDHPLHYADGM 240
N INSSSFRDPTT+WLG D WRV+IGSK+D +G+AI+Y+SK+FV W + HPLH A+G
Sbjct: 181 NKINSSSFRDPTTSWLGKDGFWRVLIGSKIDTKGMAILYKSKNFVDWVEAKHPLHSAEGT 240
Query: 241 GMWECPDFFPVVKKG--RTGIDTVVNG-PHVKHVLKISLDDTKHDLYTIGTYKLDKDIYV 300
GMWECPDF+PV+ K RTG+DT NG V+HVLK+SLDDTKHD Y IG+Y + KD++V
Sbjct: 241 GMWECPDFYPVLDKNLLRTGVDTSRNGDDDVRHVLKVSLDDTKHDHYLIGSYDVVKDVFV 300
Query: 301 PDKGSIENYNGLRYDYGKYYASKTFFDSQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIP 360
P+ G +N LRYDYGKYYASKTFFD K RR+L GWVNESSSV DD+KKGWSG+ IP
Sbjct: 301 PENGFEDNGFVLRYDYGKYYASKTFFDDGKNRRILLGWVNESSSVADDVKKGWSGIHTIP 360
Query: 361 RTVWLDASGKQLIQWPIVEIHKLRKDKVKLTNKVLKKGSVVEVKGVTAVQADVEVSFKVK 420
R +WL SGKQL+QWP+ EI LR + V KV+K G + + GV +VQADVE+SF++
Sbjct: 361 REIWLHESGKQLVQWPVKEIENLRMNPVNWPTKVIKGGERISITGVDSVQADVEISFEIS 420
Query: 421 NFNKAEVLKPEWKDPQLLCSQKGTAADGGVGPFGLLVLASKDLKEYTSVLFRIFKHYNKY 480
+ K E L+ +W DPQLLCSQKG GGVGPFGLLV AS+ LKEYT+V FRIFK+ +K
Sbjct: 421 DLGKVESLR-KWIDPQLLCSQKGAGVKGGVGPFGLLVFASQGLKEYTAVFFRIFKYQDKN 480
Query: 481 VVLLCSDQSRSSLNSNNDKTTYGAFLDVDPVSENLSLRSLIDHSIVESFGSKGKACITAR 540
+VL+CSDQSRSSLN +ND T+YG F+DVDP+ E LSLR+LIDHS+VESFG +G+AC+TAR
Sbjct: 481 LVLMCSDQSRSSLNKDNDMTSYGTFVDVDPLHEKLSLRTLIDHSVVESFGGEGRACVTAR 540
Query: 541 VYPTLAIGDKVGL 545
VYPTLAI DK L
Sbjct: 541 VYPTLAIHDKAML 549
BLAST of Spg013912 vs. ExPASy Swiss-Prot
Match:
Q70XE6 (Fructan 6-exohydrolase OS=Beta vulgaris OX=161934 GN=6-FEH PE=1 SV=1)
HSP 1 Score: 726.5 bits (1874), Expect = 2.4e-208
Identity = 358/576 (62.15%), Postives = 437/576 (75.87%), Query Frame = 0
Query: 9 ISFLGLLLGHGVLQL---QASHHVYKAQTSHFVPHDYQ-----QPYRTSYHFQPPKNWM- 68
+S +LL HG++ + QA HH + + +D+Q PYRT+YHFQ PKNWM
Sbjct: 11 LSISMMLLSHGMIIIAKDQAIHH-HDDDHDDMLINDHQMINDDDPYRTAYHFQSPKNWMN 70
Query: 69 --NGPMIFKGIYHLFYQYNPKGAVW-GNIVWAHSTSTDLINWVPHEYAIYPSQPSDINGC 128
NGPMI+KGIYHLFYQY P VW IVW HSTSTDLINW A+ PS+P DINGC
Sbjct: 71 DPNGPMIYKGIYHLFYQYYPYDPVWHTEIVWGHSTSTDLINWTQQPIALSPSEPYDINGC 130
Query: 129 WSGSATILPGAKPAILYTGINPKSQQVQNLAVPKNLSDPYLREWVKSPKNPLMA--PTPE 188
WSGS TILP KP ILYTGIN K+ QVQNLA+PKNLSDPYL+EW+K P+NPLMA PT
Sbjct: 131 WSGSITILPQNKPVILYTGINNKNYQVQNLALPKNLSDPYLKEWIKLPQNPLMAGTPTNN 190
Query: 189 NHINSSSFRDPTTAWLGPDRQWRVIIGSKVDARGLAIMYRSKDFVQWDQVDHPLHYADGM 248
N+IN+SSFRDP+TAW D +WRVI+G++ RGLA+++ S DFV+W+ +PLH +G
Sbjct: 191 NNINASSFRDPSTAWQLSDGKWRVIVGTQQGKRGLAVLFTSDDFVKWNNTGNPLHSTEGN 250
Query: 249 GMWECPDFFPVVKKGRTGIDTVVNGPHVKHVLKISLDDTKHDLYTIGTYKLDKDIYVPDK 308
G+WECPDFFPV G DT + G VKHVLK+SL DT+++ YTIG Y ++KDIYVPD+
Sbjct: 251 GIWECPDFFPVYVGKSLGADTSIIGDDVKHVLKLSLFDTQYEYYTIGRYDIEKDIYVPDE 310
Query: 309 GSIENYNGLRYDYGKYYASKTFFDSQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRTV 368
GSIE+ GLRYDYGK+YASK+FFD + RR+LWGWVNESS DDIKKGWSGVQAIPRTV
Sbjct: 311 GSIESDLGLRYDYGKFYASKSFFDDETNRRILWGWVNESSIQADDIKKGWSGVQAIPRTV 370
Query: 369 WLDASGKQLIQWPIVEIHKLRKDKVKLTNKVLKKGSVVEVKGVTAVQADVEVSFKVKNFN 428
LD SGKQL+QWP+ E+ LR++ V+L ++V+K GS+VE+ +TA QADVE+SFK+ N
Sbjct: 371 VLDKSGKQLVQWPLAEVDMLRENDVELPSQVIKGGSLVEISQITASQADVEISFKIPESN 430
Query: 429 KAEVLKPEWKDPQLLCSQKGTAADGGVGPFGLLVLASKDLKEYTSVLFRIFKHYNKYVVL 488
E L +PQ+LCSQKG + G GPFGLL LAS L+EYT+V FRIFK NKYVVL
Sbjct: 431 YVEELDSTCTNPQILCSQKGASIKGRFGPFGLLTLASMGLEEYTAVFFRIFKGPNKYVVL 490
Query: 489 LCSDQSRSSLNSNNDKTTYGAFLDVDPVSENLSLRSLIDHSIVESFGSKGKACITARVYP 548
+CSDQ+RSSLN DK ++G F+DVDP++E+LSLR LIDHSIVESF +KGK+CITARVYP
Sbjct: 491 MCSDQTRSSLNPTTDKLSFGIFVDVDPINEDLSLRILIDHSIVESFSAKGKSCITARVYP 550
Query: 549 TLAIGDKVGLYAFNNGTEPVTITKLNAWSLNKAQIS 571
T+AI DK LY FNNGTE V ITKL+AWS+ KAQI+
Sbjct: 551 TMAINDKAKLYVFNNGTEDVKITKLSAWSMKKAQIN 585
BLAST of Spg013912 vs. ExPASy Swiss-Prot
Match:
Q67XZ3 (Beta-fructofuranosidase, insoluble isoenzyme CWINV3 OS=Arabidopsis thaliana OX=3702 GN=CWINV3 PE=1 SV=2)
HSP 1 Score: 714.1 bits (1842), Expect = 1.2e-204
Identity = 348/571 (60.95%), Postives = 413/571 (72.33%), Query Frame = 0
Query: 12 LGLLLGHGVLQLQASHHVYKAQTSHFVPHDYQQPYRTSYHFQPPKNWM---NGPMIFKGI 71
L + L + + L+AS H D QPYRT YHFQP KNWM NGPMI+KGI
Sbjct: 15 LSMFLANFITDLEASSH-----------QDLNQPYRTGYHFQPLKNWMNDPNGPMIYKGI 74
Query: 72 YHLFYQYNPKGAVWG-NIVWAHSTSTDLINWVPHEYAIYPSQPSDINGCWSGSATILPGA 131
YHLFYQYNP GAVW IVW HSTS DL+NW+ A PSQPSDINGCWSGS TILP
Sbjct: 75 YHLFYQYNPYGAVWDVRIVWGHSTSVDLVNWISQPPAFNPSQPSDINGCWSGSVTILPNG 134
Query: 132 KPAILYTGINPKSQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPENHINSSSFRDPTT 191
KP ILYTGI+ QVQN+AVP N+SDPYLREW K P+NPLM N IN FRDPTT
Sbjct: 135 KPVILYTGIDQNKGQVQNVAVPVNISDPYLREWSKPPQNPLMTTNAVNGINPDRFRDPTT 194
Query: 192 AWLGPDRQWRVIIGSKV-DARGLAIMYRSKDFVQWDQVDHPLHYADGMGMWECPDFFPVV 251
AWLG D +WRVI+GS D RGLAI+Y+S+DF W Q PLHY D GMWECPDFFPV
Sbjct: 195 AWLGRDGEWRVIVGSSTDDRRGLAILYKSRDFFNWTQSMKPLHYEDLTGMWECPDFFPVS 254
Query: 252 KKGRTGIDTVVNGPH-VKHVLKISLDDTKHDLYTIGTYKLDKDIYVPDKGSIENYNGLRY 311
G G++T G + +KHVLK+SL +T HD YTIG+Y +KD+YVPD G ++N + R
Sbjct: 255 ITGSDGVETSSVGENGIKHVLKVSLIETLHDYYTIGSYDREKDVYVPDLGFVQNESAPRL 314
Query: 312 DYGKYYASKTFFDSQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRTVWLDASGKQLIQ 371
DYGKYYASKTF+D KKRR+LWGWVNESS DDI+KGWSG+Q+ PR +WLD SGK+L+Q
Sbjct: 315 DYGKYYASKTFYDDVKKRRILWGWVNESSPAKDDIEKGWSGLQSFPRKIWLDESGKELLQ 374
Query: 372 WPIVEIHKLRKDKVKLTNKVLKKGSVVEVKGVTAVQADVEVSFKVKNFNKAEVLKPEWKD 431
WPI EI LR +V KVLK GS ++V GVTA QADVEVSFKVK KA+V++P W D
Sbjct: 375 WPIEEIETLRGQQVNWQKKVLKAGSTLQVHGVTAAQADVEVSFKVKELEKADVIEPSWTD 434
Query: 432 PQLLCSQKGTAADGGVGPFGLLVLASKDLKEYTSVLFRIFKHYN------KYVVLLCSDQ 491
PQ +CSQ + G+GPFGL+VLAS D++EYTSV FRIFK + KYVVL+CSDQ
Sbjct: 435 PQKICSQGDLSVMSGLGPFGLMVLASNDMEEYTSVYFRIFKSNDDTNKKTKYVVLMCSDQ 494
Query: 492 SRSSLNSNNDKTTYGAFLDVDPVSENLSLRSLIDHSIVESFGSKGKACITARVYPTLAIG 551
SRSSLN NDK+T+GAF+ +DP + +SLR+LIDHSIVES+G G+ CIT+RVYP LAIG
Sbjct: 495 SRSSLNDENDKSTFGAFVAIDPSHQTISLRTLIDHSIVESYGGGGRTCITSRVYPKLAIG 554
Query: 552 DKVGLYAFNNGTEPVTITKLNAWSLNKAQIS 571
+ L+ FN GT+ V I L+AWSL AQI+
Sbjct: 555 ENANLFVFNKGTQSVDILTLSAWSLKSAQIN 574
BLAST of Spg013912 vs. ExPASy Swiss-Prot
Match:
Q9LIB9 (Beta-fructofuranosidase, insoluble isoenzyme CWINV5 OS=Arabidopsis thaliana OX=3702 GN=CWINV5 PE=2 SV=2)
HSP 1 Score: 701.0 bits (1808), Expect = 1.1e-200
Identity = 341/542 (62.92%), Postives = 417/542 (76.94%), Query Frame = 0
Query: 38 VPHDYQ-QPYRTSYHFQPPKNWM---NGPMIFKGIYHLFYQYNPKGAVW--GNIVWAHST 97
VP++ + QPYRT YHFQPPKNWM NGPMI+KGIYHLFYQ+N GAV VW H+T
Sbjct: 33 VPNNIKNQPYRTGYHFQPPKNWMNDPNGPMIYKGIYHLFYQWNQNGAVMDVNKTVWGHAT 92
Query: 98 STDLINWVPHEYAIYPSQPSDINGCWSGSATILPGAKPAILYTGINPKSQQVQNLAVPKN 157
STDLINW+ AI PS+PSDINGCWSGS TILP KP ILYTG + ++QVQNL PKN
Sbjct: 93 STDLINWITLSPAIRPSRPSDINGCWSGSVTILPNGKPVILYTGNDRYNRQVQNLVKPKN 152
Query: 158 LSDPYLREWVKSPKNPLMAPTPENHINSSSFRDPTTAWLGPDRQWRVIIGSKVDARGLAI 217
L+DPYLR W KSP+NPL+ P+P NHINSS+FRDPTTAW G D +WR+ GS+ RGLAI
Sbjct: 153 LTDPYLRHWTKSPENPLVTPSPVNHINSSAFRDPTTAWFGRDGRWRITTGSQEGRRGLAI 212
Query: 218 MYRSKDFVQWDQVDHPLHYADGMGMWECPDFFPVVKKGRTGIDT-VVNGPHVKHVLKISL 277
++ SKDFV W Q PLHY DG GMWECPDFFPV + G+DT +GP VKHVLK+SL
Sbjct: 213 LHTSKDFVIWKQSPKPLHYHDGTGMWECPDFFPVARTDSRGLDTSFSSGPMVKHVLKVSL 272
Query: 278 DDTKHDLYTIGTYKLDKDIYVPDKGSIENYNGLRYDYGKYYASKTFFDSQKKRRVLWGWV 337
DT +D YTIGTY +D+YVPDKG +++ RYDYGK+YASKTF+DS +RR+LWGWV
Sbjct: 273 TDTFNDYYTIGTYDEVRDVYVPDKGFVQDETAPRYDYGKFYASKTFYDSVNQRRILWGWV 332
Query: 338 NESSSVDDDIKKGWSGVQAIPRTVWLDASGKQLIQWPIVEIHKLRKDKVKLTNKVLKKGS 397
NESS D+IKKGW+G+QAIPR VWLD SGK+L+QWP+ EI +LR +VK NK+LK GS
Sbjct: 333 NESSPEKDNIKKGWAGLQAIPRKVWLDESGKRLVQWPVKEIERLRTTQVKWGNKLLKGGS 392
Query: 398 VVEVKGVTAVQADVEVSFKVKNFN--KAEVLKPEWKDPQLLCSQKGTAADGGVGPFGLLV 457
V+EV GVTA QADVEV FKV F+ KA+V++P W DPQL+CSQ+ A G+GPFGL+V
Sbjct: 393 VMEVHGVTAPQADVEVFFKVSGFDLEKADVIEPGWTDPQLICSQRN--ASSGLGPFGLMV 452
Query: 458 LASKDLKEYTSVLFRIFK---HYNKYVVLLCSDQSRSSLNSNNDKTTYGAFLDVDPVSEN 517
LASK+++EYTSV RIF+ + ++VV++CSDQS SSL NDKTTYGAFLD+ P +
Sbjct: 453 LASKNMEEYTSVNIRIFRAGENSKEHVVVMCSDQSTSSLEKGNDKTTYGAFLDISPY-QP 512
Query: 518 LSLRSLIDHSIVESFGSKGKACITARVYPTLAIGDKVGLYAFNNGTEPVTITKLNAWSLN 568
+SLR+LID SIVESFG KGK CIT+RVYP LAIG++ L+AFN G++ V + L+AWS+
Sbjct: 513 ISLRTLIDKSIVESFGGKGKTCITSRVYPKLAIGERTHLFAFNKGSQNVNVLSLSAWSMK 571
BLAST of Spg013912 vs. ExPASy TrEMBL
Match:
A0A6J1GPU7 (beta-fructofuranosidase, insoluble isoenzyme CWINV1-like OS=Cucurbita moschata OX=3662 GN=LOC111456399 PE=3 SV=1)
HSP 1 Score: 1027.7 bits (2656), Expect = 1.8e-296
Identity = 491/573 (85.69%), Postives = 522/573 (91.10%), Query Frame = 0
Query: 1 MASLSLWFISFLGLLLGHGVLQLQASHHVYKAQTSHFVPHDYQQPYRTSYHFQPPKNWM- 60
MAS SL FISFL LL HG L+LQASHHVY Q S + H Y+QPYRTSYHFQPPKNW+
Sbjct: 1 MASSSLCFISFLCFLLCHGALKLQASHHVYTPQPSRLLLHRYEQPYRTSYHFQPPKNWIN 60
Query: 61 --NGPMIFKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWVPHEYAIYPSQPSDINGCW 120
NGPMI+KGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINW PH+YAIYPSQPSDINGCW
Sbjct: 61 DPNGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWKPHDYAIYPSQPSDINGCW 120
Query: 121 SGSATILPGAKPAILYTGINPKSQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPENHI 180
SGSATILPG KPAILYTGINPK+QQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTP+NHI
Sbjct: 121 SGSATILPGDKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQNHI 180
Query: 181 NSSSFRDPTTAWLGPDRQWRVIIGSKVDARGLAIMYRSKDFVQWDQVDHPLHYADGMGMW 240
NSSSFRDPTTAWLG D +WRVIIGSK+DARGLAIM++SKDFV+WDQVDHPLHYADG GMW
Sbjct: 181 NSSSFRDPTTAWLGHDGEWRVIIGSKIDARGLAIMFQSKDFVKWDQVDHPLHYADGTGMW 240
Query: 241 ECPDFFPVVKKGRTGIDTVVNGPHVKHVLKISLDDTKHDLYTIGTYKLDKDIYVPDKGSI 300
ECPDFFPV K R G+DT V+G HVKHVLK+SLDDTKHD YT+GTY L KDIYVPDKGSI
Sbjct: 241 ECPDFFPVAKNSRAGVDTSVSGQHVKHVLKVSLDDTKHDQYTVGTYDLKKDIYVPDKGSI 300
Query: 301 ENYNGLRYDYGKYYASKTFFDSQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRTVWLD 360
E Y GLRYDYGKYYASKTFFD QKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPR VWLD
Sbjct: 301 EGYTGLRYDYGKYYASKTFFDGQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRAVWLD 360
Query: 361 ASGKQLIQWPIVEIHKLRKDKVKLTNKVLKKGSVVEVKGVTAVQADVEVSFKVKNFNKAE 420
ASGKQLIQWPIVEI KLRKDKVKLTNKVLKKGS++ VKGVTA QADV+VSFKVK+FNKA+
Sbjct: 361 ASGKQLIQWPIVEIQKLRKDKVKLTNKVLKKGSIIAVKGVTAAQADVDVSFKVKSFNKAQ 420
Query: 421 VLKPEWKDPQLLCSQKGTAADGGVGPFGLLVLASKDLKEYTSVLFRIFKHYNKYVVLLCS 480
LK EWKDPQL+CSQ G AA GGVGPFGLLVLAS +L+EYTSV FRIFK +KYVVL+CS
Sbjct: 421 PLKAEWKDPQLVCSQMGAAAKGGVGPFGLLVLASNNLEEYTSVFFRIFKRQDKYVVLMCS 480
Query: 481 DQSRSSLNSNNDKTTYGAFLDVDPVSENLSLRSLIDHSIVESFGSKGKACITARVYPTLA 540
DQSRSSLN+NNDKTTYGAFLDVDPV EN+SLRSLIDHSIVESFGSKGKACITARVYP LA
Sbjct: 481 DQSRSSLNANNDKTTYGAFLDVDPVRENISLRSLIDHSIVESFGSKGKACITARVYPRLA 540
Query: 541 IGDKVGLYAFNNGTEPVTITKLNAWSLNKAQIS 571
IGDK GLYAFNNGTE VTITKL+AWS+ KAQIS
Sbjct: 541 IGDKAGLYAFNNGTESVTITKLSAWSMKKAQIS 573
BLAST of Spg013912 vs. ExPASy TrEMBL
Match:
A0A6J1GPY1 (beta-fructofuranosidase, insoluble isoenzyme CWINV1-like OS=Cucurbita moschata OX=3662 GN=LOC111456400 PE=3 SV=1)
HSP 1 Score: 1021.5 bits (2640), Expect = 1.3e-294
Identity = 487/572 (85.14%), Postives = 522/572 (91.26%), Query Frame = 0
Query: 1 MASLSLWFISFLGLLLGHGVLQLQASHHVYKAQTSHFVPHDYQQPYRTSYHFQPPKNWM- 60
MAS SL FISFL LL HG L+LQASHHVY Q S + H ++QPYRTS HFQPPKNW+
Sbjct: 1 MASSSLCFISFLCFLLCHGALKLQASHHVYTPQPSRLLLHHHEQPYRTSCHFQPPKNWIN 60
Query: 61 --NGPMIFKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWVPHEYAIYPSQPSDINGCW 120
NGPMI+KGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINW PH+YAIYPSQPSDINGCW
Sbjct: 61 DPNGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWKPHDYAIYPSQPSDINGCW 120
Query: 121 SGSATILPGAKPAILYTGINPKSQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPENHI 180
SGSATILPG KPAILYTGINPK+QQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTP+NHI
Sbjct: 121 SGSATILPGDKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQNHI 180
Query: 181 NSSSFRDPTTAWLGPDRQWRVIIGSKVDARGLAIMYRSKDFVQWDQVDHPLHYADGMGMW 240
NSSSFRDPTTAWLGPD +WRVIIGSKVD RGLAIM+RSKDFV+WDQVDHPLHYADGMGMW
Sbjct: 181 NSSSFRDPTTAWLGPDGEWRVIIGSKVDDRGLAIMFRSKDFVKWDQVDHPLHYADGMGMW 240
Query: 241 ECPDFFPVVKKGRTGIDTVVNGPHVKHVLKISLDDTKHDLYTIGTYKLDKDIYVPDKGSI 300
ECPDFFPV K GR G+DT V+G HVKHVLK+SLDDTKHD YT+GTY L KDIYVPDKGSI
Sbjct: 241 ECPDFFPVAKNGRAGVDTSVSGQHVKHVLKVSLDDTKHDQYTVGTYDLKKDIYVPDKGSI 300
Query: 301 ENYNGLRYDYGKYYASKTFFDSQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRTVWLD 360
++Y+GLRYDYGKYYASKTFFD QKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRTVWLD
Sbjct: 301 QDYSGLRYDYGKYYASKTFFDGQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRTVWLD 360
Query: 361 ASGKQLIQWPIVEIHKLRKDKVKLTNKVLKKGSVVEVKGVTAVQADVEVSFKVKNFNKAE 420
ASGKQLIQWPI EI KLRKDK+KLTNKVL+KGS++ VKGVTA QADV+VSFKVK+FNKA
Sbjct: 361 ASGKQLIQWPIAEIQKLRKDKIKLTNKVLEKGSIIAVKGVTAAQADVDVSFKVKSFNKAH 420
Query: 421 VLKPEWKDPQLLCSQKGTAADGGVGPFGLLVLASKDLKEYTSVLFRIFKHYNKYVVLLCS 480
+LK EWKDPQL+CSQ G AA GGVGPFGLLVLAS +L+EYTSV FRIFK +KYVVL+CS
Sbjct: 421 LLKAEWKDPQLVCSQMGAAAKGGVGPFGLLVLASNNLEEYTSVFFRIFKRQDKYVVLMCS 480
Query: 481 DQSRSSLNSNNDKTTYGAFLDVDPVSENLSLRSLIDHSIVESFGSKGKACITARVYPTLA 540
DQSRSSLN++NDKT YGAFLDVDPV +N+SLRSLIDHSIVESFGSKGKACITARVYP LA
Sbjct: 481 DQSRSSLNADNDKTMYGAFLDVDPVRKNVSLRSLIDHSIVESFGSKGKACITARVYPRLA 540
Query: 541 IGDKVGLYAFNNGTEPVTITKLNAWSLNKAQI 570
IGDK GLYAFNNGTE VTITKL AWSL KAQI
Sbjct: 541 IGDKAGLYAFNNGTESVTITKLRAWSLKKAQI 572
BLAST of Spg013912 vs. ExPASy TrEMBL
Match:
A0A6J1DM23 (beta-fructofuranosidase, insoluble isoenzyme CWINV1-like OS=Momordica charantia OX=3673 GN=LOC111021302 PE=3 SV=1)
HSP 1 Score: 1017.3 bits (2629), Expect = 2.5e-293
Identity = 479/573 (83.60%), Postives = 523/573 (91.27%), Query Frame = 0
Query: 1 MASLSLWFISFLGLLLGHGVLQLQASHHVYKAQTSHFVPHDYQQPYRTSYHFQPPKNWM- 60
MAS SLWF SFL + L HGV +L+ASHHVY+AQTS VP D+QQPYRTSYHFQPPKNWM
Sbjct: 1 MASFSLWFFSFLSVFLCHGVFKLEASHHVYRAQTSASVPPDHQQPYRTSYHFQPPKNWMN 60
Query: 61 --NGPMIFKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWVPHEYAIYPSQPSDINGCW 120
NGPMI+KGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWVPH++AI+PSQPSDINGCW
Sbjct: 61 DPNGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWVPHQHAIFPSQPSDINGCW 120
Query: 121 SGSATILPGAKPAILYTGINPKSQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPENHI 180
SGSATILPG KPA+LYTGINP++ QVQNLAVPKNLSDP+L EWVKSPKNPLMAPTP+N+I
Sbjct: 121 SGSATILPGNKPAMLYTGINPQNHQVQNLAVPKNLSDPFLIEWVKSPKNPLMAPTPQNNI 180
Query: 181 NSSSFRDPTTAWLGPDRQWRVIIGSKVDARGLAIMYRSKDFVQWDQVDHPLHYADGMGMW 240
NSSSFRDPTTAWLG DRQWRV+IGSK++ARGLAIMYRSKDF+QWDQV++PLHYAD GMW
Sbjct: 181 NSSSFRDPTTAWLGRDRQWRVVIGSKINARGLAIMYRSKDFIQWDQVNNPLHYADDTGMW 240
Query: 241 ECPDFFPVVKKGRTGIDTVVNGPHVKHVLKISLDDTKHDLYTIGTYKLDKDIYVPDKGSI 300
ECPDFFPV K R G+DT VNGP VKHVLK+SLDDTKHD YT+GTY + KD+YVPDKGS+
Sbjct: 241 ECPDFFPVAKNSRVGVDTSVNGPRVKHVLKVSLDDTKHDHYTVGTYNVKKDVYVPDKGSV 300
Query: 301 ENYNGLRYDYGKYYASKTFFDSQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRTVWLD 360
E Y GLRYDYGK YASKTFFDS+KKRRVLWGWVNESSSVDDD KKGWSGVQAIPR++WLD
Sbjct: 301 EGYAGLRYDYGKCYASKTFFDSEKKRRVLWGWVNESSSVDDDKKKGWSGVQAIPRSIWLD 360
Query: 361 ASGKQLIQWPIVEIHKLRKDKVKLTNKVLKKGSVVEVKGVTAVQADVEVSFKVKNFNKAE 420
ASGKQL+QWPIVEI KLRKDKVKL NKVLKKGSV+EVKGVTA Q DVEVSFKVKNF KA+
Sbjct: 361 ASGKQLVQWPIVEIQKLRKDKVKLINKVLKKGSVIEVKGVTAAQVDVEVSFKVKNFKKAQ 420
Query: 421 VLKPEWKDPQLLCSQKGTAADGGVGPFGLLVLASKDLKEYTSVLFRIFKHYNKYVVLLCS 480
VLKPEWKDPQLLC+QKG A + GVGPFGLL+LASKDLKEYTSV F++FK KYVVL+CS
Sbjct: 421 VLKPEWKDPQLLCAQKGAAKESGVGPFGLLLLASKDLKEYTSVFFKVFKREGKYVVLMCS 480
Query: 481 DQSRSSLNSNNDKTTYGAFLDVDPVSENLSLRSLIDHSIVESFGSKGKACITARVYPTLA 540
DQSRSSLNSNNDKTTYGAF+D+DPV ENLSLRSLIDHSIVESFG KGKACITARVYPTLA
Sbjct: 481 DQSRSSLNSNNDKTTYGAFVDMDPVRENLSLRSLIDHSIVESFGVKGKACITARVYPTLA 540
Query: 541 IGDKVGLYAFNNGTEPVTITKLNAWSLNKAQIS 571
IG+ VGLYAFNN TE VTI+KL AWSLNKA I+
Sbjct: 541 IGENVGLYAFNNATEGVTISKLTAWSLNKAHIN 573
BLAST of Spg013912 vs. ExPASy TrEMBL
Match:
A0A6J1JP62 (beta-fructofuranosidase, insoluble isoenzyme CWINV1-like OS=Cucurbita maxima OX=3661 GN=LOC111488605 PE=3 SV=1)
HSP 1 Score: 1010.4 bits (2611), Expect = 3.0e-291
Identity = 483/573 (84.29%), Postives = 515/573 (89.88%), Query Frame = 0
Query: 1 MASLSLWFISFLGLLLGHGVLQLQASHHVYKAQTSHFVPHDYQQPYRTSYHFQPPKNWM- 60
MAS SL FIS L LL HG L+LQASHHVY AQ S + H Y+QPYRTSYHFQPPKNW+
Sbjct: 3 MASSSLCFISLLCFLLCHGALKLQASHHVYTAQASRLLLHHYEQPYRTSYHFQPPKNWIN 62
Query: 61 --NGPMIFKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWVPHEYAIYPSQPSDINGCW 120
NGPMI+KGIYHLFYQYNPKGA WGNIVWAHSTSTDLINW PH++AIYPSQPSDINGCW
Sbjct: 63 DPNGPMIYKGIYHLFYQYNPKGATWGNIVWAHSTSTDLINWKPHDHAIYPSQPSDINGCW 122
Query: 121 SGSATILPGAKPAILYTGINPKSQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPENHI 180
SGSATILPG KPAILYTGINPK+QQVQNLA+PKNLSDPYLREWVKSP NPLMAPTP+N I
Sbjct: 123 SGSATILPGEKPAILYTGINPKNQQVQNLALPKNLSDPYLREWVKSPNNPLMAPTPQNRI 182
Query: 181 NSSSFRDPTTAWLGPDRQWRVIIGSKVDARGLAIMYRSKDFVQWDQVDHPLHYADGMGMW 240
NSSSFRDPTTAWLG D QWRVIIGSK+DARGLAIM+RSKDFV+WDQVDHPLHYADG GMW
Sbjct: 183 NSSSFRDPTTAWLGHDGQWRVIIGSKIDARGLAIMFRSKDFVRWDQVDHPLHYADGTGMW 242
Query: 241 ECPDFFPVVKKGRTGIDTVVNGPHVKHVLKISLDDTKHDLYTIGTYKLDKDIYVPDKGSI 300
ECPDFFPV K GR GIDT VNG HVKHVLK+SLDDTKHD YT+GTY L KDIYVPDK S
Sbjct: 243 ECPDFFPVAKNGRAGIDTSVNGGHVKHVLKVSLDDTKHDHYTVGTYDLKKDIYVPDKEST 302
Query: 301 ENYNGLRYDYGKYYASKTFFDSQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRTVWLD 360
E Y GLRYDYGKYYASKTFFD QKKRRVLWGWVNESSSVDDD+KKGWSGVQAIPR VWLD
Sbjct: 303 EGYTGLRYDYGKYYASKTFFDGQKKRRVLWGWVNESSSVDDDMKKGWSGVQAIPRAVWLD 362
Query: 361 ASGKQLIQWPIVEIHKLRKDKVKLTNKVLKKGSVVEVKGVTAVQADVEVSFKVKNFNKAE 420
ASGKQLIQWPI EI KLRKDK+KL NKV+KKGS++ VKGVTA QADVEVSFKVK+F KA+
Sbjct: 363 ASGKQLIQWPIAEIQKLRKDKIKLRNKVVKKGSIIAVKGVTAAQADVEVSFKVKSFKKAQ 422
Query: 421 VLKPEWKDPQLLCSQKGTAADGGVGPFGLLVLASKDLKEYTSVLFRIFKHYNKYVVLLCS 480
LK EWKDPQL CS+ G AA GGVGPFGLLVLASKDL+EYTSV FR+FK +KYVVL+CS
Sbjct: 423 RLKAEWKDPQLACSRMGAAAMGGVGPFGLLVLASKDLEEYTSVFFRVFKGQDKYVVLMCS 482
Query: 481 DQSRSSLNSNNDKTTYGAFLDVDPVSENLSLRSLIDHSIVESFGSKGKACITARVYPTLA 540
DQSRSSLN+NNDKTTYGAFLDVDPV EN+SLRSLIDHSIVESFGSKGKACITARVYP LA
Sbjct: 483 DQSRSSLNANNDKTTYGAFLDVDPVRENISLRSLIDHSIVESFGSKGKACITARVYPKLA 542
Query: 541 IGDKVGLYAFNNGTEPVTITKLNAWSLNKAQIS 571
+GDK GLYAFNNGTE VTITKL+AWS+ KAQIS
Sbjct: 543 MGDKAGLYAFNNGTERVTITKLSAWSMKKAQIS 575
BLAST of Spg013912 vs. ExPASy TrEMBL
Match:
A0A2I4JRK2 (Cell wall invertase 2 OS=Cucumis sativus OX=3659 PE=2 SV=1)
HSP 1 Score: 984.6 bits (2544), Expect = 1.8e-283
Identity = 473/581 (81.41%), Postives = 516/581 (88.81%), Query Frame = 0
Query: 3 SLSLWFIS----FLGLLLGHGVLQLQASHHVYKA--QTSHFVPHDY-QQPYRTSYHFQPP 62
S SL F++ + LL GH LQLQA HHVY+A +TSHFV H + QQPYRTSYHFQPP
Sbjct: 5 SSSLCFLNNIFLLIFLLFGHEFLQLQALHHVYRASHRTSHFVLHQHQQQPYRTSYHFQPP 64
Query: 63 KNWM---NGPMIFKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWVPHEYAIYPSQPSD 122
KNW+ NGPMI+KGIYH FYQYNPKGAVWGNIVWAHSTSTDLINW PH++AI+PSQPSD
Sbjct: 65 KNWINDPNGPMIYKGIYHFFYQYNPKGAVWGNIVWAHSTSTDLINWEPHDHAIFPSQPSD 124
Query: 123 INGCWSGSATILPGAKPAILYTGINPKSQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPT 182
INGCWSGSATILPG KPAILYTGINPK+QQVQNLAVPKNLSDPYLREWVKSPKNPLMAPT
Sbjct: 125 INGCWSGSATILPGEKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPT 184
Query: 183 PENHINSSSFRDPTTAWLGPDRQWRVIIGSKVDARGLAIMYRSKDFVQWDQVDHPLHYAD 242
P+NHIN SSFRDPTTAWLG D +WRVIIGSK+DARGLAIMYRSKDFV+W+QVDHPL Y D
Sbjct: 185 PQNHINVSSFRDPTTAWLGRDGEWRVIIGSKIDARGLAIMYRSKDFVRWNQVDHPLRYKD 244
Query: 243 GMGMWECPDFFPVVKKGRTGIDTVVNGPHVKHVLKISLDDTKHDLYTIGTYKLDKDIYVP 302
MGMWECPDFFPV K GRTG++ V+G HVKH+LK+SLDDTKHD+YT+GTY KDIY+P
Sbjct: 245 DMGMWECPDFFPVAKTGRTGVEMKVSGSHVKHILKVSLDDTKHDVYTVGTYNWKKDIYIP 304
Query: 303 DKGSIENYNGLRYDYGKYYASKTFFDSQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPR 362
+KGSIE YNGLRYDYGKYYASKTFFDSQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPR
Sbjct: 305 NKGSIEGYNGLRYDYGKYYASKTFFDSQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPR 364
Query: 363 TVWLDASGKQLIQWPIVEIHKLRKDKVKLTNKVLKKGSVVEVKGVTAVQADVEVSFKVKN 422
+WLDASGKQLIQWPIVEI KLR +KVKLTN+VLKKGS +EVKGVTA QADVE+SFKV+N
Sbjct: 365 AIWLDASGKQLIQWPIVEIQKLRNNKVKLTNEVLKKGSTIEVKGVTAAQADVEISFKVEN 424
Query: 423 FNKAEVLKPEWKDPQLLCSQKGTAA---DGGVGPFGLLVLASKDLKEYTSVLFRIFKHYN 482
KAE+LKPEWKDPQLLCSQKGT++ G VGPFGLLVLAS DLKEYTSV F +FK +
Sbjct: 425 LKKAEILKPEWKDPQLLCSQKGTSSTTEGGSVGPFGLLVLASNDLKEYTSVFFTVFKKPH 484
Query: 483 KYVVLLCSDQSRSSLNSNNDKTTYGAFLDVDPVSENLSLRSLIDHSIVESFGSKGKACIT 542
KYVVL+CSDQSRSSL+SNNDKTTYGAFLDVDP EN+SLRSLIDHSIVESFG KGK CIT
Sbjct: 485 KYVVLMCSDQSRSSLHSNNDKTTYGAFLDVDPTRENISLRSLIDHSIVESFGIKGKGCIT 544
Query: 543 ARVYPTLAIGDKVGLYAFNNGTEPVTITKLNAWSLNKAQIS 571
ARVYPTLAIGD V LYAFNNGT+ VTITKL AWS+ KA I+
Sbjct: 545 ARVYPTLAIGDNVSLYAFNNGTDSVTITKLTAWSMKKAHIN 585
BLAST of Spg013912 vs. TAIR 10
Match:
AT3G13790.2 (Glycosyl hydrolases family 32 protein )
HSP 1 Score: 805.8 bits (2080), Expect = 2.2e-233
Identity = 391/578 (67.65%), Postives = 462/578 (79.93%), Query Frame = 0
Query: 2 ASLSLWFISFLGLLLGHGVLQLQASHHVYK--AQTSHFVPHDYQQPYRTSYHFQPPKNWM 61
+++ LW + L LL+G+ V+ L+ASHHVYK Q+++ QPYRT +HFQPPKNWM
Sbjct: 7 SNIGLWLL--LTLLIGNYVVNLEASHHVYKRLTQSTNTKSPSVNQPYRTGFHFQPPKNWM 66
Query: 62 NGPMIFKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWVPHEYAIYPSQPSDINGCWSG 121
NGPMI+KGIYHLFYQ+NPKGAVWGNIVWAHSTSTDLINW PH AI+PS P DINGCWSG
Sbjct: 67 NGPMIYKGIYHLFYQWNPKGAVWGNIVWAHSTSTDLINWDPHPPAIFPSAPFDINGCWSG 126
Query: 122 SATILPGAKPAILYTGINPKSQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPENHINS 181
SATILP KP ILYTGI+PK+QQVQN+A PKNLSDPYLREW KSP NPLMAP N IN+
Sbjct: 127 SATILPNGKPVILYTGIDPKNQQVQNIAEPKNLSDPYLREWKKSPLNPLMAPDAVNGINA 186
Query: 182 SSFRDPTTAWLGPDRQWRVIIGSKVDARGLAIMYRSKDFVQWDQVDHPLHYADGMGMWEC 241
SSFRDPTTAWLG D++WRVIIGSK+ RGLAI Y SKDF++W++ PLHY DG GMWEC
Sbjct: 187 SSFRDPTTAWLGQDKKWRVIIGSKIHRRGLAITYTSKDFLKWEKSPEPLHYDDGSGMWEC 246
Query: 242 PDFFPVVKKGRTGIDTVVNG-PH--VKHVLKISLDDTKHDLYTIGTYKLDKDIYVPDKGS 301
PDFFPV + G G++T G P+ +KHVLKISLDDTKHD YTIGTY KD +VPD G
Sbjct: 247 PDFFPVTRFGSNGVETSSFGEPNEILKHVLKISLDDTKHDYYTIGTYDRVKDKFVPDNGF 306
Query: 302 IENYNGLRYDYGKYYASKTFFDSQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRTVWL 361
+ RYDYGKYYASKTFFDS K RR+LWGW NESSSV+DD++KGWSG+Q IPR +WL
Sbjct: 307 KMDGTAPRYDYGKYYASKTFFDSAKNRRILWGWTNESSSVEDDVEKGWSGIQTIPRKIWL 366
Query: 362 DASGKQLIQWPIVEIHKLRKDKVK-LTNKVLKKGSVVEVKGVTAVQADVEVSFKVKNFNK 421
D SGKQLIQWP+ E+ +LR +VK L NKVLK GS +EV GVTA QADVEV FKV++ K
Sbjct: 367 DRSGKQLIQWPVREVERLRTKQVKNLRNKVLKSGSRLEVYGVTAAQADVEVLFKVRDLEK 426
Query: 422 AEVLKPEWKDPQLLCSQKGTAADGGVGPFGLLVLASKDLKEYTSVLFRIFK---HYNKYV 481
A+V++P W DPQL+CS+ + G+GPFGL+VLASK+L+EYTSV FRIFK + NKYV
Sbjct: 427 ADVIEPSWTDPQLICSKMNVSVKSGLGPFGLMVLASKNLEEYTSVYFRIFKARQNSNKYV 486
Query: 482 VLLCSDQSRSSLNSNNDKTTYGAFLDVDPVSENLSLRSLIDHSIVESFGSKGKACITARV 541
VL+CSDQSRSSL +NDKTTYGAF+D++P + LSLR+LIDHS+VESFG KG+ACIT+RV
Sbjct: 487 VLMCSDQSRSSLKEDNDKTTYGAFVDINP-HQPLSLRALIDHSVVESFGGKGRACITSRV 546
Query: 542 YPTLAIGDKVGLYAFNNGTEPVTITKLNAWSLNKAQIS 571
YP LAIG L+AFN G + V + LNAWS+N AQIS
Sbjct: 547 YPKLAIGKSSHLFAFNYGYQSVDVLNLNAWSMNSAQIS 581
BLAST of Spg013912 vs. TAIR 10
Match:
AT3G13790.1 (Glycosyl hydrolases family 32 protein )
HSP 1 Score: 800.4 bits (2066), Expect = 9.2e-232
Identity = 391/581 (67.30%), Postives = 462/581 (79.52%), Query Frame = 0
Query: 2 ASLSLWFISFLGLLLGHGVLQLQASHHVYK--AQTSHFVPHDYQQPYRTSYHFQPPKNWM 61
+++ LW + L LL+G+ V+ L+ASHHVYK Q+++ QPYRT +HFQPPKNWM
Sbjct: 7 SNIGLWLL--LTLLIGNYVVNLEASHHVYKRLTQSTNTKSPSVNQPYRTGFHFQPPKNWM 66
Query: 62 ---NGPMIFKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWVPHEYAIYPSQPSDINGC 121
NGPMI+KGIYHLFYQ+NPKGAVWGNIVWAHSTSTDLINW PH AI+PS P DINGC
Sbjct: 67 NDPNGPMIYKGIYHLFYQWNPKGAVWGNIVWAHSTSTDLINWDPHPPAIFPSAPFDINGC 126
Query: 122 WSGSATILPGAKPAILYTGINPKSQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPENH 181
WSGSATILP KP ILYTGI+PK+QQVQN+A PKNLSDPYLREW KSP NPLMAP N
Sbjct: 127 WSGSATILPNGKPVILYTGIDPKNQQVQNIAEPKNLSDPYLREWKKSPLNPLMAPDAVNG 186
Query: 182 INSSSFRDPTTAWLGPDRQWRVIIGSKVDARGLAIMYRSKDFVQWDQVDHPLHYADGMGM 241
IN+SSFRDPTTAWLG D++WRVIIGSK+ RGLAI Y SKDF++W++ PLHY DG GM
Sbjct: 187 INASSFRDPTTAWLGQDKKWRVIIGSKIHRRGLAITYTSKDFLKWEKSPEPLHYDDGSGM 246
Query: 242 WECPDFFPVVKKGRTGIDTVVNG-PH--VKHVLKISLDDTKHDLYTIGTYKLDKDIYVPD 301
WECPDFFPV + G G++T G P+ +KHVLKISLDDTKHD YTIGTY KD +VPD
Sbjct: 247 WECPDFFPVTRFGSNGVETSSFGEPNEILKHVLKISLDDTKHDYYTIGTYDRVKDKFVPD 306
Query: 302 KGSIENYNGLRYDYGKYYASKTFFDSQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRT 361
G + RYDYGKYYASKTFFDS K RR+LWGW NESSSV+DD++KGWSG+Q IPR
Sbjct: 307 NGFKMDGTAPRYDYGKYYASKTFFDSAKNRRILWGWTNESSSVEDDVEKGWSGIQTIPRK 366
Query: 362 VWLDASGKQLIQWPIVEIHKLRKDKVK-LTNKVLKKGSVVEVKGVTAVQADVEVSFKVKN 421
+WLD SGKQLIQWP+ E+ +LR +VK L NKVLK GS +EV GVTA QADVEV FKV++
Sbjct: 367 IWLDRSGKQLIQWPVREVERLRTKQVKNLRNKVLKSGSRLEVYGVTAAQADVEVLFKVRD 426
Query: 422 FNKAEVLKPEWKDPQLLCSQKGTAADGGVGPFGLLVLASKDLKEYTSVLFRIFK---HYN 481
KA+V++P W DPQL+CS+ + G+GPFGL+VLASK+L+EYTSV FRIFK + N
Sbjct: 427 LEKADVIEPSWTDPQLICSKMNVSVKSGLGPFGLMVLASKNLEEYTSVYFRIFKARQNSN 486
Query: 482 KYVVLLCSDQSRSSLNSNNDKTTYGAFLDVDPVSENLSLRSLIDHSIVESFGSKGKACIT 541
KYVVL+CSDQSRSSL +NDKTTYGAF+D++P + LSLR+LIDHS+VESFG KG+ACIT
Sbjct: 487 KYVVLMCSDQSRSSLKEDNDKTTYGAFVDINP-HQPLSLRALIDHSVVESFGGKGRACIT 546
Query: 542 ARVYPTLAIGDKVGLYAFNNGTEPVTITKLNAWSLNKAQIS 571
+RVYP LAIG L+AFN G + V + LNAWS+N AQIS
Sbjct: 547 SRVYPKLAIGKSSHLFAFNYGYQSVDVLNLNAWSMNSAQIS 584
BLAST of Spg013912 vs. TAIR 10
Match:
AT1G55120.1 (beta-fructofuranosidase 5 )
HSP 1 Score: 714.1 bits (1842), Expect = 8.7e-206
Identity = 348/571 (60.95%), Postives = 413/571 (72.33%), Query Frame = 0
Query: 12 LGLLLGHGVLQLQASHHVYKAQTSHFVPHDYQQPYRTSYHFQPPKNWM---NGPMIFKGI 71
L + L + + L+AS H D QPYRT YHFQP KNWM NGPMI+KGI
Sbjct: 15 LSMFLANFITDLEASSH-----------QDLNQPYRTGYHFQPLKNWMNDPNGPMIYKGI 74
Query: 72 YHLFYQYNPKGAVWG-NIVWAHSTSTDLINWVPHEYAIYPSQPSDINGCWSGSATILPGA 131
YHLFYQYNP GAVW IVW HSTS DL+NW+ A PSQPSDINGCWSGS TILP
Sbjct: 75 YHLFYQYNPYGAVWDVRIVWGHSTSVDLVNWISQPPAFNPSQPSDINGCWSGSVTILPNG 134
Query: 132 KPAILYTGINPKSQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPENHINSSSFRDPTT 191
KP ILYTGI+ QVQN+AVP N+SDPYLREW K P+NPLM N IN FRDPTT
Sbjct: 135 KPVILYTGIDQNKGQVQNVAVPVNISDPYLREWSKPPQNPLMTTNAVNGINPDRFRDPTT 194
Query: 192 AWLGPDRQWRVIIGSKV-DARGLAIMYRSKDFVQWDQVDHPLHYADGMGMWECPDFFPVV 251
AWLG D +WRVI+GS D RGLAI+Y+S+DF W Q PLHY D GMWECPDFFPV
Sbjct: 195 AWLGRDGEWRVIVGSSTDDRRGLAILYKSRDFFNWTQSMKPLHYEDLTGMWECPDFFPVS 254
Query: 252 KKGRTGIDTVVNGPH-VKHVLKISLDDTKHDLYTIGTYKLDKDIYVPDKGSIENYNGLRY 311
G G++T G + +KHVLK+SL +T HD YTIG+Y +KD+YVPD G ++N + R
Sbjct: 255 ITGSDGVETSSVGENGIKHVLKVSLIETLHDYYTIGSYDREKDVYVPDLGFVQNESAPRL 314
Query: 312 DYGKYYASKTFFDSQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRTVWLDASGKQLIQ 371
DYGKYYASKTF+D KKRR+LWGWVNESS DDI+KGWSG+Q+ PR +WLD SGK+L+Q
Sbjct: 315 DYGKYYASKTFYDDVKKRRILWGWVNESSPAKDDIEKGWSGLQSFPRKIWLDESGKELLQ 374
Query: 372 WPIVEIHKLRKDKVKLTNKVLKKGSVVEVKGVTAVQADVEVSFKVKNFNKAEVLKPEWKD 431
WPI EI LR +V KVLK GS ++V GVTA QADVEVSFKVK KA+V++P W D
Sbjct: 375 WPIEEIETLRGQQVNWQKKVLKAGSTLQVHGVTAAQADVEVSFKVKELEKADVIEPSWTD 434
Query: 432 PQLLCSQKGTAADGGVGPFGLLVLASKDLKEYTSVLFRIFKHYN------KYVVLLCSDQ 491
PQ +CSQ + G+GPFGL+VLAS D++EYTSV FRIFK + KYVVL+CSDQ
Sbjct: 435 PQKICSQGDLSVMSGLGPFGLMVLASNDMEEYTSVYFRIFKSNDDTNKKTKYVVLMCSDQ 494
Query: 492 SRSSLNSNNDKTTYGAFLDVDPVSENLSLRSLIDHSIVESFGSKGKACITARVYPTLAIG 551
SRSSLN NDK+T+GAF+ +DP + +SLR+LIDHSIVES+G G+ CIT+RVYP LAIG
Sbjct: 495 SRSSLNDENDKSTFGAFVAIDPSHQTISLRTLIDHSIVESYGGGGRTCITSRVYPKLAIG 554
Query: 552 DKVGLYAFNNGTEPVTITKLNAWSLNKAQIS 571
+ L+ FN GT+ V I L+AWSL AQI+
Sbjct: 555 ENANLFVFNKGTQSVDILTLSAWSLKSAQIN 574
BLAST of Spg013912 vs. TAIR 10
Match:
AT3G13784.1 (cell wall invertase 5 )
HSP 1 Score: 706.4 bits (1822), Expect = 1.8e-203
Identity = 341/539 (63.27%), Postives = 417/539 (77.37%), Query Frame = 0
Query: 38 VPHDYQ-QPYRTSYHFQPPKNWMNGPMIFKGIYHLFYQYNPKGAVW--GNIVWAHSTSTD 97
VP++ + QPYRT YHFQPPKNWMNGPMI+KGIYHLFYQ+N GAV VW H+TSTD
Sbjct: 33 VPNNIKNQPYRTGYHFQPPKNWMNGPMIYKGIYHLFYQWNQNGAVMDVNKTVWGHATSTD 92
Query: 98 LINWVPHEYAIYPSQPSDINGCWSGSATILPGAKPAILYTGINPKSQQVQNLAVPKNLSD 157
LINW+ AI PS+PSDINGCWSGS TILP KP ILYTG + ++QVQNL PKNL+D
Sbjct: 93 LINWITLSPAIRPSRPSDINGCWSGSVTILPNGKPVILYTGNDRYNRQVQNLVKPKNLTD 152
Query: 158 PYLREWVKSPKNPLMAPTPENHINSSSFRDPTTAWLGPDRQWRVIIGSKVDARGLAIMYR 217
PYLR W KSP+NPL+ P+P NHINSS+FRDPTTAW G D +WR+ GS+ RGLAI++
Sbjct: 153 PYLRHWTKSPENPLVTPSPVNHINSSAFRDPTTAWFGRDGRWRITTGSQEGRRGLAILHT 212
Query: 218 SKDFVQWDQVDHPLHYADGMGMWECPDFFPVVKKGRTGIDT-VVNGPHVKHVLKISLDDT 277
SKDFV W Q PLHY DG GMWECPDFFPV + G+DT +GP VKHVLK+SL DT
Sbjct: 213 SKDFVIWKQSPKPLHYHDGTGMWECPDFFPVARTDSRGLDTSFSSGPMVKHVLKVSLTDT 272
Query: 278 KHDLYTIGTYKLDKDIYVPDKGSIENYNGLRYDYGKYYASKTFFDSQKKRRVLWGWVNES 337
+D YTIGTY +D+YVPDKG +++ RYDYGK+YASKTF+DS +RR+LWGWVNES
Sbjct: 273 FNDYYTIGTYDEVRDVYVPDKGFVQDETAPRYDYGKFYASKTFYDSVNQRRILWGWVNES 332
Query: 338 SSVDDDIKKGWSGVQAIPRTVWLDASGKQLIQWPIVEIHKLRKDKVKLTNKVLKKGSVVE 397
S D+IKKGW+G+QAIPR VWLD SGK+L+QWP+ EI +LR +VK NK+LK GSV+E
Sbjct: 333 SPEKDNIKKGWAGLQAIPRKVWLDESGKRLVQWPVKEIERLRTTQVKWGNKLLKGGSVME 392
Query: 398 VKGVTAVQADVEVSFKVKNFN--KAEVLKPEWKDPQLLCSQKGTAADGGVGPFGLLVLAS 457
V GVTA QADVEV FKV F+ KA+V++P W DPQL+CSQ+ A G+GPFGL+VLAS
Sbjct: 393 VHGVTAPQADVEVFFKVSGFDLEKADVIEPGWTDPQLICSQRN--ASSGLGPFGLMVLAS 452
Query: 458 KDLKEYTSVLFRIFK---HYNKYVVLLCSDQSRSSLNSNNDKTTYGAFLDVDPVSENLSL 517
K+++EYTSV RIF+ + ++VV++CSDQS SSL NDKTTYGAFLD+ P + +SL
Sbjct: 453 KNMEEYTSVNIRIFRAGENSKEHVVVMCSDQSTSSLEKGNDKTTYGAFLDISPY-QPISL 512
Query: 518 RSLIDHSIVESFGSKGKACITARVYPTLAIGDKVGLYAFNNGTEPVTITKLNAWSLNKA 568
R+LID SIVESFG KGK CIT+RVYP LAIG++ L+AFN G++ V + L+AWS+ +
Sbjct: 513 RTLIDKSIVESFGGKGKTCITSRVYPKLAIGERTHLFAFNKGSQNVNVLSLSAWSMKSS 568
BLAST of Spg013912 vs. TAIR 10
Match:
AT1G55120.3 (beta-fructofuranosidase 5 )
HSP 1 Score: 683.3 bits (1762), Expect = 1.6e-196
Identity = 327/525 (62.29%), Postives = 390/525 (74.29%), Query Frame = 0
Query: 55 PKNWMNGPMIFKGIYHLFYQYNPKGAVWG-NIVWAHSTSTDLINWVPHEYAIYPSQPSDI 114
P ++GPMI+KGIYHLFYQYNP GAVW IVW HSTS DL+NW+ A PSQPSDI
Sbjct: 35 PNVSVSGPMIYKGIYHLFYQYNPYGAVWDVRIVWGHSTSVDLVNWISQPPAFNPSQPSDI 94
Query: 115 NGCWSGSATILPGAKPAILYTGINPKSQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTP 174
NGCWSGS TILP KP ILYTGI+ QVQN+AVP N+SDPYLREW K P+NPLM
Sbjct: 95 NGCWSGSVTILPNGKPVILYTGIDQNKGQVQNVAVPVNISDPYLREWSKPPQNPLMTTNA 154
Query: 175 ENHINSSSFRDPTTAWLGPDRQWRVIIGSKV-DARGLAIMYRSKDFVQWDQVDHPLHYAD 234
N IN FRDPTTAWLG D +WRVI+GS D RGLAI+Y+S+DF W Q PLHY D
Sbjct: 155 VNGINPDRFRDPTTAWLGRDGEWRVIVGSSTDDRRGLAILYKSRDFFNWTQSMKPLHYED 214
Query: 235 GMGMWECPDFFPVVKKGRTGIDTVVNGPH-VKHVLKISLDDTKHDLYTIGTYKLDKDIYV 294
GMWECPDFFPV G G++T G + +KHVLK+SL +T HD YTIG+Y +KD+YV
Sbjct: 215 LTGMWECPDFFPVSITGSDGVETSSVGENGIKHVLKVSLIETLHDYYTIGSYDREKDVYV 274
Query: 295 PDKGSIENYNGLRYDYGKYYASKTFFDSQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIP 354
PD G ++N + R DYGKYYASKTF+D KKRR+LWGWVNESS DDI+KGWSG+Q+ P
Sbjct: 275 PDLGFVQNESAPRLDYGKYYASKTFYDDVKKRRILWGWVNESSPAKDDIEKGWSGLQSFP 334
Query: 355 RTVWLDASGKQLIQWPIVEIHKLRKDKVKLTNKVLKKGSVVEVKGVTAVQADVEVSFKVK 414
R +WLD SGK+L+QWPI EI LR +V KVLK GS ++V GVTA QADVEVSFKVK
Sbjct: 335 RKIWLDESGKELLQWPIEEIETLRGQQVNWQKKVLKAGSTLQVHGVTAAQADVEVSFKVK 394
Query: 415 NFNKAEVLKPEWKDPQLLCSQKGTAADGGVGPFGLLVLASKDLKEYTSVLFRIFKHYN-- 474
KA+V++P W DPQ +CSQ + G+GPFGL+VLAS D++EYTSV FRIFK +
Sbjct: 395 ELEKADVIEPSWTDPQKICSQGDLSVMSGLGPFGLMVLASNDMEEYTSVYFRIFKSNDDT 454
Query: 475 ----KYVVLLCSDQSRSSLNSNNDKTTYGAFLDVDPVSENLSLRSLIDHSIVESFGSKGK 534
KYVVL+CSDQSRSSLN NDK+T+GAF+ +DP + +SLR+LIDHSIVES+G G+
Sbjct: 455 NKKTKYVVLMCSDQSRSSLNDENDKSTFGAFVAIDPSHQTISLRTLIDHSIVESYGGGGR 514
Query: 535 ACITARVYPTLAIGDKVGLYAFNNGTEPVTITKLNAWSLNKAQIS 571
CIT+RVYP LAIG+ L+ FN GT+ V I L+AWSL AQI+
Sbjct: 515 TCITSRVYPKLAIGENANLFVFNKGTQSVDILTLSAWSLKSAQIN 559
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
KAG6575469.1 | 5.7e-300 | 86.67 | Beta-fructofuranosidase, insoluble isoenzyme CWINV1, partial [Cucurbita argyrosp... | [more] |
XP_038898203.1 | 1.3e-299 | 85.86 | beta-fructofuranosidase, insoluble isoenzyme CWINV1-like [Benincasa hispida] | [more] |
XP_023547447.1 | 2.9e-296 | 85.84 | beta-fructofuranosidase, insoluble isoenzyme CWINV1-like [Cucurbita pepo subsp. ... | [more] |
XP_022954011.1 | 3.8e-296 | 85.69 | beta-fructofuranosidase, insoluble isoenzyme CWINV1-like [Cucurbita moschata] >X... | [more] |
KAG6575468.1 | 5.0e-296 | 85.76 | Beta-fructofuranosidase, insoluble isoenzyme CWINV1, partial [Cucurbita argyrosp... | [more] |
Match Name | E-value | Identity | Description | |
Q43866 | 1.3e-230 | 67.30 | Beta-fructofuranosidase, insoluble isoenzyme CWINV1 OS=Arabidopsis thaliana OX=3... | [more] |
Q43089 | 7.1e-213 | 64.20 | Beta-fructofuranosidase, cell wall isozyme OS=Pisum sativum OX=3888 GN=BFRUCT1 P... | [more] |
Q70XE6 | 2.4e-208 | 62.15 | Fructan 6-exohydrolase OS=Beta vulgaris OX=161934 GN=6-FEH PE=1 SV=1 | [more] |
Q67XZ3 | 1.2e-204 | 60.95 | Beta-fructofuranosidase, insoluble isoenzyme CWINV3 OS=Arabidopsis thaliana OX=3... | [more] |
Q9LIB9 | 1.1e-200 | 62.92 | Beta-fructofuranosidase, insoluble isoenzyme CWINV5 OS=Arabidopsis thaliana OX=3... | [more] |
Match Name | E-value | Identity | Description | |
A0A6J1GPU7 | 1.8e-296 | 85.69 | beta-fructofuranosidase, insoluble isoenzyme CWINV1-like OS=Cucurbita moschata O... | [more] |
A0A6J1GPY1 | 1.3e-294 | 85.14 | beta-fructofuranosidase, insoluble isoenzyme CWINV1-like OS=Cucurbita moschata O... | [more] |
A0A6J1DM23 | 2.5e-293 | 83.60 | beta-fructofuranosidase, insoluble isoenzyme CWINV1-like OS=Momordica charantia ... | [more] |
A0A6J1JP62 | 3.0e-291 | 84.29 | beta-fructofuranosidase, insoluble isoenzyme CWINV1-like OS=Cucurbita maxima OX=... | [more] |
A0A2I4JRK2 | 1.8e-283 | 81.41 | Cell wall invertase 2 OS=Cucumis sativus OX=3659 PE=2 SV=1 | [more] |