Spg013912 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg013912
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Descriptionbeta-fructofuranosidase, insoluble isoenzyme CWINV1-like
Locationscaffold3: 49044600 .. 49048362 (+)
RNA-Seq ExpressionSpg013912
SyntenySpg013912
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AATATATTGTTGAACCAAACCCAATAAGTCATCTTCTTCAAATCCTTCTCCAATGGCTTCCTTGTCTCTCTGGTTCATCTCTTTCCTTGGTCTCTTGCTTGGCCATGGAGTTTTGCAGCTTCAAGCTTCCCACCATGTTTACAAAGCTCAAACTTCTCACTTTGTTCCTCATGATTATCAACAACCCTATAGAACTTCTTACCATTTTCAACCTCCAAAGAATTGGATGAATGGTGTTTTTTTTTTTTTCACTCTTTTTCTTCATCTTTTTTTTTTTTTTTTTTTTTTTTGGCTTGTGGGTTTTCTTATTTTTCTAAAAGTTTGCAACTTTTTTCTTTGACATGTGATTTGGCTATGGAATTCAATGGAAAAACTCAGATCCAAATGGTTAGTAGTTGATCTCTTCTTTGTTGTTCTTCATTTTTTAAAAAAATTTTACTTGATTTGTCAAATTTAAAATTACAAATTTGGAACTTTTGGATTATTGTCTCCATTATTGAAATTTCAAAACCAAAATTATAATTGAAATTTTAAAACCCATTATTTCATTGTTCATTTCCATTTTTGGGATATTTCAGGTCCAATGATATTCAAGGGAATTTATCACCTGTTTTACCAATACAACCCAAAAGGAGCAGTGTGGGGCAACATTGTGTGGGCCCACTCCACATCAACGGACCTGATCAACTGGGTACCCCACGAGTACGCCATCTATCCATCTCAGCCGTCGGATATCAACGGCTGCTGGTCGGGCTCCGCCACCATCCTCCCCGGCGCCAAACCGGCGATTCTCTACACAGGAATTAACCCTAAAAGCCAACAAGTTCAAAACTTGGCTGTCCCCAAGAATCTCTCTGATCCATATCTAAGAGAATGGGTGAAATCCCCAAAAAACCCTCTAATGGCCCCCACCCCTGAGAATCACATCAACTCCAGCTCCTTCCGGGACCCCACCACCGCCTGGCTCGGCCCCGATCGGCAATGGCGAGTCATCATCGGGAGCAAAGTTGACGCCCGTGGACTCGCCATCATGTACCGAAGCAAGGATTTCGTCCAGTGGGATCAGGTCGTGTTAAATTACCACTAAACTCAAAAGCACTTGATCATATGTTTTTTTAATTATTCAAACCTCTGACTCTATGGTCGACGAAATAATTATAGGTTGACCACCCGCTTCATTATGCCGATGGCATGGGAATGTGGGAGTGTCCGGATTTCTTTCCGGTGGTGAAGAAAGGTCGGACCGGCATCGACACGGTGGTGAACGGACCGCACGTGAAGCACGTGTTGAAGATAAGCTTGGATGATACTAAGCATGACCTTTATACTATTGGAACATATAAGTTGGATAAGGATATCTATGTTCCAGATAAAGGATCCATAGAGAATTACAATGGATTGAGGTATGATTATGGGAAATATTATGCTTCAAAAACTTTTTTTGACAGCCAAAAGAAAAGGAGAGTTTTGTGGGGTTGGGTGAATGAATCTTCAAGTGTTGATGATGATATCAAGAAAGGATGGTCTGGAGTCCAGGTAATTAATTAATTAATTAATTAAGTCAATTATTGAATTCAACTCTACTTTTTCAGCTTTTAGAAAAATATAAAAAGAAGGAAAAAAAAAAGTCTTCATCTGCCGACATGATGGAGTGATGGATTTGAATTCTATTTTGTTTTTATTTTTGCCTTTTAAACAAATCTATTTTAATTTCTAGATTTTTTCTATTACTAAACTTTTAAAAATTAGCTATTTTGATTCACACTATTATTTGGTTTCGTAAACGAAACACTAGGTTTTATCGATGGATCAATTTAGCACTTTTACATGCTCGTGTCAGTTCATTTTTTGCACATATTTGAAGTTATGTTATCGATTGTTAAAAAGATAAAGGATAAAAATCAATTAGTGATTTCTTTTTTTCTTGAAATACTGAGACTAAAATAGATATTTTTGGTAATTCAAGGATCAAAATGGAATAAGTTCAAAATATAAGTGGGTGGAAAGTTTTTCTCGTTAGACTCACATGTGGGGTTGATGAGAGAGATTACATTTACATTATGTAGGCAATTCCAAGGACGGTTTGGTTGGATGCATCTGGAAAGCAATTGATTCAATGGCCGATCGTGGAAATTCATAAGCTTCGAAAGGACAAAGTTAAGTTGACGAACAAAGTGTTGAAGAAAGGATCAGTGGTTGAAGTAAAAGGAGTAACAGCAGTGCAGGTGACTAAACATAAGAGACGGTGTTAAAGTCGTTTCATTTGGTTTTTGGTTTTTGATTTTTTTGTTTTTTAATTACAGGCAGATGTTGAGGTTTCATTTAAAGTAAAAAACTTCAACAAAGCTGAAGTGTTGAAACCTGAATGGAAAGACCCACAATTGCTTTGTAGCCAAAAGGGAACAGCTGCAGATGGTGGAGTAGGCCCATTTGGGCTTCTAGTTTTGGCTTCAAAGGACTTGAAAGAGTACACTTCAGTGTTGTTTAGGATATTCAAACACTACAACAAATACGTCGTGCTTTTGTGTAGTGACCAAAGCAGGTACGATAAATTATTTGAGAGGTTGGAACAATGTGGCGTAAAGTTTCACACATTTTGATGCATTTTTTTGTGGTGACTAGGTCTTCGTTGAACTCAAATAATGACAAGACAACGTACGGAGCATTCTTAGATGTCGATCCAGTTAGTGAAAATCTCTCGCTAAGAAGCTTGGTGAGATTCTTTTTCATTCTATTATATATAAGGGTTTTTAAGAAAGGAATTTTGTTCTCCACTATGATTTTTGAGCTGAATTTATGGAATCAATTGGTTCAAAACACTCAACTAGGTTTGCTTTTAGGACAAAGAAGTTGTTCAAAAAGTGCACTTATCCATGTATTCAAACACCATATTCTAATCAATTAAAAAAACTCCATAAAGTTGTGTGACACATTCAATCCCACTTAGACTTTTGGTAGGACTTACAAAGTGAGTCTTGATATTCATATTTATTGAATAAAAAATCGATCTTTCTTATCTTTTATCAACAGGGTCTTAAGTTTATTCAAATGAACAAATAAAATACCAATTGATTCATATACATAGATCTCGAATCTATGAATAAGGACGTTCTTGAAACTCGTGTAGTAAAAAACTAAGTAGTTAGACAAAAAGTAAAAGAGATTTAGGCCAAAGTTATTTGTATTATATTTGACAGCTTTTGTTGTTACCAATGTAAAGCATTTGACCATTTTTGTCAACTAATGCACATGAAAAATTCTAAAACTTTTACAGATTGATCACTCAATTGTTGAGAGCTTTGGATCAAAAGGGAAGGCTTGCATCACAGCTAGAGTTTATCCAACATTGGCCATTGGAGACAAGGTTGGTCTCTATGCTTTCAACAATGGAACAGAGCCTGTCACAATCACAAAACTGAATGCTTGGAGCTTGAACAAAGCTCAAATAAGTTGAACCAAAAGCCAACAAGTGGGAAGGAAATGAGGAAGAGGGAGACTTTTAACCATTTGTTTCTCATTTTTACAGTGATAAGCCAATTGGCAATTAAATAAAAAGGCTGTGTGATCCATGTGATTAGTCTGCCTTCTTGTGTGAAGGCTGGGTAATCCATGTGTTTTGCTTTTGCCCCTAATTCATGTGTGAAGGCTGTGTCATCCATGTGATTAGTCTGCCTTCTTTTTGTAGCTCAACTTCTCATTTGTTTTCTTATGAAGATAAAGACCAATTAAAATAGAGGGAGACCCACATTTTATGTTACTTGAG

mRNA sequence

ATGGCTTCCTTGTCTCTCTGGTTCATCTCTTTCCTTGGTCTCTTGCTTGGCCATGGAGTTTTGCAGCTTCAAGCTTCCCACCATGTTTACAAAGCTCAAACTTCTCACTTTGTTCCTCATGATTATCAACAACCCTATAGAACTTCTTACCATTTTCAACCTCCAAAGAATTGGATGAATGGTCCAATGATATTCAAGGGAATTTATCACCTGTTTTACCAATACAACCCAAAAGGAGCAGTGTGGGGCAACATTGTGTGGGCCCACTCCACATCAACGGACCTGATCAACTGGGTACCCCACGAGTACGCCATCTATCCATCTCAGCCGTCGGATATCAACGGCTGCTGGTCGGGCTCCGCCACCATCCTCCCCGGCGCCAAACCGGCGATTCTCTACACAGGAATTAACCCTAAAAGCCAACAAGTTCAAAACTTGGCTGTCCCCAAGAATCTCTCTGATCCATATCTAAGAGAATGGGTGAAATCCCCAAAAAACCCTCTAATGGCCCCCACCCCTGAGAATCACATCAACTCCAGCTCCTTCCGGGACCCCACCACCGCCTGGCTCGGCCCCGATCGGCAATGGCGAGTCATCATCGGGAGCAAAGTTGACGCCCGTGGACTCGCCATCATGTACCGAAGCAAGGATTTCGTCCAGTGGGATCAGGTTGACCACCCGCTTCATTATGCCGATGGCATGGGAATGTGGGAGTGTCCGGATTTCTTTCCGGTGGTGAAGAAAGGTCGGACCGGCATCGACACGGTGGTGAACGGACCGCACGTGAAGCACGTGTTGAAGATAAGCTTGGATGATACTAAGCATGACCTTTATACTATTGGAACATATAAGTTGGATAAGGATATCTATGTTCCAGATAAAGGATCCATAGAGAATTACAATGGATTGAGGTATGATTATGGGAAATATTATGCTTCAAAAACTTTTTTTGACAGCCAAAAGAAAAGGAGAGTTTTGTGGGGTTGGGTGAATGAATCTTCAAGTGTTGATGATGATATCAAGAAAGGATGGTCTGGAGTCCAGGCAATTCCAAGGACGGTTTGGTTGGATGCATCTGGAAAGCAATTGATTCAATGGCCGATCGTGGAAATTCATAAGCTTCGAAAGGACAAAGTTAAGTTGACGAACAAAGTGTTGAAGAAAGGATCAGTGGTTGAAGTAAAAGGAGTAACAGCAGTGCAGGCAGATGTTGAGGTTTCATTTAAAGTAAAAAACTTCAACAAAGCTGAAGTGTTGAAACCTGAATGGAAAGACCCACAATTGCTTTGTAGCCAAAAGGGAACAGCTGCAGATGGTGGAGTAGGCCCATTTGGGCTTCTAGTTTTGGCTTCAAAGGACTTGAAAGAGTACACTTCAGTGTTGTTTAGGATATTCAAACACTACAACAAATACGTCGTGCTTTTGTGTAGTGACCAAAGCAGGTCTTCGTTGAACTCAAATAATGACAAGACAACGTACGGAGCATTCTTAGATGTCGATCCAGTTAGTGAAAATCTCTCGCTAAGAAGCTTGATTGATCACTCAATTGTTGAGAGCTTTGGATCAAAAGGGAAGGCTTGCATCACAGCTAGAGTTTATCCAACATTGGCCATTGGAGACAAGGTTGGTCTCTATGCTTTCAACAATGGAACAGAGCCTGTCACAATCACAAAACTGAATGCTTGGAGCTTGAACAAAGCTCAAATAAGTTGA

Coding sequence (CDS)

ATGGCTTCCTTGTCTCTCTGGTTCATCTCTTTCCTTGGTCTCTTGCTTGGCCATGGAGTTTTGCAGCTTCAAGCTTCCCACCATGTTTACAAAGCTCAAACTTCTCACTTTGTTCCTCATGATTATCAACAACCCTATAGAACTTCTTACCATTTTCAACCTCCAAAGAATTGGATGAATGGTCCAATGATATTCAAGGGAATTTATCACCTGTTTTACCAATACAACCCAAAAGGAGCAGTGTGGGGCAACATTGTGTGGGCCCACTCCACATCAACGGACCTGATCAACTGGGTACCCCACGAGTACGCCATCTATCCATCTCAGCCGTCGGATATCAACGGCTGCTGGTCGGGCTCCGCCACCATCCTCCCCGGCGCCAAACCGGCGATTCTCTACACAGGAATTAACCCTAAAAGCCAACAAGTTCAAAACTTGGCTGTCCCCAAGAATCTCTCTGATCCATATCTAAGAGAATGGGTGAAATCCCCAAAAAACCCTCTAATGGCCCCCACCCCTGAGAATCACATCAACTCCAGCTCCTTCCGGGACCCCACCACCGCCTGGCTCGGCCCCGATCGGCAATGGCGAGTCATCATCGGGAGCAAAGTTGACGCCCGTGGACTCGCCATCATGTACCGAAGCAAGGATTTCGTCCAGTGGGATCAGGTTGACCACCCGCTTCATTATGCCGATGGCATGGGAATGTGGGAGTGTCCGGATTTCTTTCCGGTGGTGAAGAAAGGTCGGACCGGCATCGACACGGTGGTGAACGGACCGCACGTGAAGCACGTGTTGAAGATAAGCTTGGATGATACTAAGCATGACCTTTATACTATTGGAACATATAAGTTGGATAAGGATATCTATGTTCCAGATAAAGGATCCATAGAGAATTACAATGGATTGAGGTATGATTATGGGAAATATTATGCTTCAAAAACTTTTTTTGACAGCCAAAAGAAAAGGAGAGTTTTGTGGGGTTGGGTGAATGAATCTTCAAGTGTTGATGATGATATCAAGAAAGGATGGTCTGGAGTCCAGGCAATTCCAAGGACGGTTTGGTTGGATGCATCTGGAAAGCAATTGATTCAATGGCCGATCGTGGAAATTCATAAGCTTCGAAAGGACAAAGTTAAGTTGACGAACAAAGTGTTGAAGAAAGGATCAGTGGTTGAAGTAAAAGGAGTAACAGCAGTGCAGGCAGATGTTGAGGTTTCATTTAAAGTAAAAAACTTCAACAAAGCTGAAGTGTTGAAACCTGAATGGAAAGACCCACAATTGCTTTGTAGCCAAAAGGGAACAGCTGCAGATGGTGGAGTAGGCCCATTTGGGCTTCTAGTTTTGGCTTCAAAGGACTTGAAAGAGTACACTTCAGTGTTGTTTAGGATATTCAAACACTACAACAAATACGTCGTGCTTTTGTGTAGTGACCAAAGCAGGTCTTCGTTGAACTCAAATAATGACAAGACAACGTACGGAGCATTCTTAGATGTCGATCCAGTTAGTGAAAATCTCTCGCTAAGAAGCTTGATTGATCACTCAATTGTTGAGAGCTTTGGATCAAAAGGGAAGGCTTGCATCACAGCTAGAGTTTATCCAACATTGGCCATTGGAGACAAGGTTGGTCTCTATGCTTTCAACAATGGAACAGAGCCTGTCACAATCACAAAACTGAATGCTTGGAGCTTGAACAAAGCTCAAATAAGTTGA

Protein sequence

MASLSLWFISFLGLLLGHGVLQLQASHHVYKAQTSHFVPHDYQQPYRTSYHFQPPKNWMNGPMIFKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWVPHEYAIYPSQPSDINGCWSGSATILPGAKPAILYTGINPKSQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPENHINSSSFRDPTTAWLGPDRQWRVIIGSKVDARGLAIMYRSKDFVQWDQVDHPLHYADGMGMWECPDFFPVVKKGRTGIDTVVNGPHVKHVLKISLDDTKHDLYTIGTYKLDKDIYVPDKGSIENYNGLRYDYGKYYASKTFFDSQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRTVWLDASGKQLIQWPIVEIHKLRKDKVKLTNKVLKKGSVVEVKGVTAVQADVEVSFKVKNFNKAEVLKPEWKDPQLLCSQKGTAADGGVGPFGLLVLASKDLKEYTSVLFRIFKHYNKYVVLLCSDQSRSSLNSNNDKTTYGAFLDVDPVSENLSLRSLIDHSIVESFGSKGKACITARVYPTLAIGDKVGLYAFNNGTEPVTITKLNAWSLNKAQIS
Homology
BLAST of Spg013912 vs. NCBI nr
Match: KAG6575469.1 (Beta-fructofuranosidase, insoluble isoenzyme CWINV1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1040.4 bits (2689), Expect = 5.7e-300
Identity = 494/570 (86.67%), Postives = 526/570 (92.28%), Query Frame = 0

Query: 1   MASLSLWFISFLGLLLGHGVLQLQASHHVYKAQTSHFVPHDYQQPYRTSYHFQPPKNWMN 60
           MAS SL FISFL  LL HG L+LQASHHVY+AQ S  + H Y+QPYRTSYHFQPPKNW+N
Sbjct: 1   MASSSLCFISFLCFLLCHGALKLQASHHVYRAQASRLLLHRYEQPYRTSYHFQPPKNWIN 60

Query: 61  GPMIFKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWVPHEYAIYPSQPSDINGCWSGS 120
           GPMI+KGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINW PH+YAIYPSQPSDINGCWSGS
Sbjct: 61  GPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWKPHDYAIYPSQPSDINGCWSGS 120

Query: 121 ATILPGAKPAILYTGINPKSQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPENHINSS 180
           ATILPG KPAILYTGINPK+QQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTP+N INSS
Sbjct: 121 ATILPGDKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQNQINSS 180

Query: 181 SFRDPTTAWLGPDRQWRVIIGSKVDARGLAIMYRSKDFVQWDQVDHPLHYADGMGMWECP 240
           SFRDPTTAWLGPD +WRVIIGSK+DARGLAIM++SKDFV+WDQVDHPLHYADG GMWECP
Sbjct: 181 SFRDPTTAWLGPDSEWRVIIGSKIDARGLAIMFQSKDFVKWDQVDHPLHYADGTGMWECP 240

Query: 241 DFFPVVKKGRTGIDTVVNGPHVKHVLKISLDDTKHDLYTIGTYKLDKDIYVPDKGSIENY 300
           DFFPV K GR G+DT V+G HVKHVLK+SLDDTKHD YT+GTY L KDIYVPDKGSIE Y
Sbjct: 241 DFFPVAKNGRAGVDTSVSGRHVKHVLKVSLDDTKHDHYTVGTYDLKKDIYVPDKGSIEGY 300

Query: 301 NGLRYDYGKYYASKTFFDSQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRTVWLDASG 360
            GLRYDYGKYYASKTFFDSQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPR VWLDASG
Sbjct: 301 TGLRYDYGKYYASKTFFDSQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRAVWLDASG 360

Query: 361 KQLIQWPIVEIHKLRKDKVKLTNKVLKKGSVVEVKGVTAVQADVEVSFKVKNFNKAEVLK 420
           KQLIQWPIVEI KLRKDKVKLTNKVLKKGS++ VKGVTA QADV+VSFKVK+FNKA+ LK
Sbjct: 361 KQLIQWPIVEIQKLRKDKVKLTNKVLKKGSIIAVKGVTAAQADVDVSFKVKSFNKAQPLK 420

Query: 421 PEWKDPQLLCSQKGTAADGGVGPFGLLVLASKDLKEYTSVLFRIFKHYNKYVVLLCSDQS 480
            EWKDPQL+CSQ G AA GGVGPFGLLVLAS +L+EYTSV FRIFK  +KYVVL+CSDQS
Sbjct: 421 AEWKDPQLVCSQMGAAAKGGVGPFGLLVLASNNLEEYTSVFFRIFKRQDKYVVLMCSDQS 480

Query: 481 RSSLNSNNDKTTYGAFLDVDPVSENLSLRSLIDHSIVESFGSKGKACITARVYPTLAIGD 540
           RSSLN+NNDKTTYGAFLDVDPV EN+SLRSLIDHSIVESFGSKGKACITARVYP LAIGD
Sbjct: 481 RSSLNANNDKTTYGAFLDVDPVRENISLRSLIDHSIVESFGSKGKACITARVYPRLAIGD 540

Query: 541 KVGLYAFNNGTEPVTITKLNAWSLNKAQIS 571
           K GLYAFNNGTE VTITKL+AWS+ KAQIS
Sbjct: 541 KAGLYAFNNGTESVTITKLSAWSMKKAQIS 570

BLAST of Spg013912 vs. NCBI nr
Match: XP_038898203.1 (beta-fructofuranosidase, insoluble isoenzyme CWINV1-like [Benincasa hispida])

HSP 1 Score: 1039.3 bits (2686), Expect = 1.3e-299
Identity = 492/573 (85.86%), Postives = 527/573 (91.97%), Query Frame = 0

Query: 1   MASLSLWFISFLGLLLGHGVLQLQASHHVYKAQTSHFVPHDYQQPYRTSYHFQPPKNWM- 60
           MAS SL FISFL  L GHGVLQLQASHHVY+AQ S+ +PH YQQPYRTSYHFQPPKNW+ 
Sbjct: 1   MASFSLCFISFLCFLFGHGVLQLQASHHVYRAQLSNSLPHHYQQPYRTSYHFQPPKNWIN 60

Query: 61  --NGPMIFKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWVPHEYAIYPSQPSDINGCW 120
             NGPMI+KGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINW PH++AIYPSQPSDINGCW
Sbjct: 61  DPNGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWEPHDHAIYPSQPSDINGCW 120

Query: 121 SGSATILPGAKPAILYTGINPKSQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPENHI 180
           SGSATILPG KPAILYTGINPK+QQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTP+NHI
Sbjct: 121 SGSATILPGDKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQNHI 180

Query: 181 NSSSFRDPTTAWLGPDRQWRVIIGSKVDARGLAIMYRSKDFVQWDQVDHPLHYADGMGMW 240
           NSSSFRDPTTAWLGPD +WRVIIGSK++ARGLAIMY+SKDFV+WDQVDHPLHYADG GMW
Sbjct: 181 NSSSFRDPTTAWLGPDGEWRVIIGSKINARGLAIMYQSKDFVKWDQVDHPLHYADGTGMW 240

Query: 241 ECPDFFPVVKKGRTGIDTVVNGPHVKHVLKISLDDTKHDLYTIGTYKLDKDIYVPDKGSI 300
           ECPDFFPV K GR G+DT V+G HVKHVLK+SLDDTKHD YT+G Y L+KDIYVP+KGSI
Sbjct: 241 ECPDFFPVAKTGRKGVDTSVSGGHVKHVLKVSLDDTKHDHYTVGNYDLEKDIYVPNKGSI 300

Query: 301 ENYNGLRYDYGKYYASKTFFDSQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRTVWLD 360
           E Y GLRYDYGKYYASKTF+D QKKRRVLWGWVNESSSV+DDIKKGWSG+QAIPRT+WLD
Sbjct: 301 EGYMGLRYDYGKYYASKTFYDGQKKRRVLWGWVNESSSVEDDIKKGWSGIQAIPRTIWLD 360

Query: 361 ASGKQLIQWPIVEIHKLRKDKVKLTNKVLKKGSVVEVKGVTAVQADVEVSFKVKNFNKAE 420
           ASGKQLIQWPI EI KLRKDKVKLTNKVLKKGS++EVKGVTA QADVEVSFKVKNF KA+
Sbjct: 361 ASGKQLIQWPIEEIQKLRKDKVKLTNKVLKKGSLIEVKGVTAAQADVEVSFKVKNFKKAQ 420

Query: 421 VLKPEWKDPQLLCSQKGTAADGGVGPFGLLVLASKDLKEYTSVLFRIFKHYNKYVVLLCS 480
           +LKPEWKDPQLLCSQKG    GGVG FGLLVLASKDLKEYTS+ F IFK   KY+VL+CS
Sbjct: 421 ILKPEWKDPQLLCSQKGATTKGGVGSFGLLVLASKDLKEYTSISFTIFKRQGKYIVLMCS 480

Query: 481 DQSRSSLNSNNDKTTYGAFLDVDPVSENLSLRSLIDHSIVESFGSKGKACITARVYPTLA 540
           DQSRSSLN NNDKTTYGAFL+VDPVSE+LSLRSLIDHS+VESFGSKGKACITARVYPTLA
Sbjct: 481 DQSRSSLNPNNDKTTYGAFLNVDPVSEDLSLRSLIDHSVVESFGSKGKACITARVYPTLA 540

Query: 541 IGDKVGLYAFNNGTEPVTITKLNAWSLNKAQIS 571
           IGD VGLY FNNGTE VTITKL AWSL KAQI+
Sbjct: 541 IGDNVGLYVFNNGTESVTITKLGAWSLKKAQIN 573

BLAST of Spg013912 vs. NCBI nr
Match: XP_023547447.1 (beta-fructofuranosidase, insoluble isoenzyme CWINV1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1028.1 bits (2657), Expect = 2.9e-296
Identity = 491/572 (85.84%), Postives = 521/572 (91.08%), Query Frame = 0

Query: 1   MASLSLWFISFLGLLLGHGVLQLQASHHVYKAQTSHFVPHDYQQPYRTSYHFQPPKNWM- 60
           MAS SL FISFL  LL HG L+LQASHHVY AQ S  + H ++QPYRTSYHFQPPKNW+ 
Sbjct: 1   MASSSLCFISFLCFLLAHGALKLQASHHVYTAQASRLLLHHHEQPYRTSYHFQPPKNWIN 60

Query: 61  --NGPMIFKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWVPHEYAIYPSQPSDINGCW 120
             NGPMI+KGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINW PH+YAIYPSQPSDINGCW
Sbjct: 61  DPNGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWKPHDYAIYPSQPSDINGCW 120

Query: 121 SGSATILPGAKPAILYTGINPKSQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPENHI 180
           SGSATILPG KPAILYTGINPK+QQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTP+NHI
Sbjct: 121 SGSATILPGDKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQNHI 180

Query: 181 NSSSFRDPTTAWLGPDRQWRVIIGSKVDARGLAIMYRSKDFVQWDQVDHPLHYADGMGMW 240
           NSSSFRDPTTAWLG D +WRVIIGSK+DARGLAIM+RSKDFV+WDQVDHPLHYADGMGMW
Sbjct: 181 NSSSFRDPTTAWLGHDGEWRVIIGSKIDARGLAIMFRSKDFVKWDQVDHPLHYADGMGMW 240

Query: 241 ECPDFFPVVKKGRTGIDTVVNGPHVKHVLKISLDDTKHDLYTIGTYKLDKDIYVPDKGSI 300
           ECPDFFPV K G  G+DT V+G HVKHVLK+SLDDTKHD YT+GTY L KDIYVPDKGSI
Sbjct: 241 ECPDFFPVAKNGHAGVDTSVSGQHVKHVLKVSLDDTKHDQYTVGTYDLKKDIYVPDKGSI 300

Query: 301 ENYNGLRYDYGKYYASKTFFDSQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRTVWLD 360
           E+Y GLRYDYGKYYASKTFFD QKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPR VWLD
Sbjct: 301 EDYTGLRYDYGKYYASKTFFDGQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRAVWLD 360

Query: 361 ASGKQLIQWPIVEIHKLRKDKVKLTNKVLKKGSVVEVKGVTAVQADVEVSFKVKNFNKAE 420
           ASGKQLIQWPI EI KLRKDK+KLTNKVLKKGS+V VKGVTA QADV+VSFKVK+FNKA+
Sbjct: 361 ASGKQLIQWPIAEIQKLRKDKIKLTNKVLKKGSIVAVKGVTAAQADVDVSFKVKSFNKAQ 420

Query: 421 VLKPEWKDPQLLCSQKGTAADGGVGPFGLLVLASKDLKEYTSVLFRIFKHYNKYVVLLCS 480
            LK EWKDPQL+CSQ G AA GGVGPFGLLVLAS +L+EYTSV FRIFK  +KYVVL+CS
Sbjct: 421 RLKAEWKDPQLVCSQMGAAAKGGVGPFGLLVLASNNLEEYTSVFFRIFKGQDKYVVLMCS 480

Query: 481 DQSRSSLNSNNDKTTYGAFLDVDPVSENLSLRSLIDHSIVESFGSKGKACITARVYPTLA 540
           DQSRSSLN+NNDKTTYGAFLDVDPV EN+SLRSLIDHSIVESFGSKGKACITARVYP LA
Sbjct: 481 DQSRSSLNANNDKTTYGAFLDVDPVRENVSLRSLIDHSIVESFGSKGKACITARVYPRLA 540

Query: 541 IGDKVGLYAFNNGTEPVTITKLNAWSLNKAQI 570
           IGDK GLYAFNNGTE VTI KL AWSL KAQI
Sbjct: 541 IGDKAGLYAFNNGTESVTIAKLRAWSLKKAQI 572

BLAST of Spg013912 vs. NCBI nr
Match: XP_022954011.1 (beta-fructofuranosidase, insoluble isoenzyme CWINV1-like [Cucurbita moschata] >XP_022954012.1 beta-fructofuranosidase, insoluble isoenzyme CWINV1-like [Cucurbita moschata])

HSP 1 Score: 1027.7 bits (2656), Expect = 3.8e-296
Identity = 491/573 (85.69%), Postives = 522/573 (91.10%), Query Frame = 0

Query: 1   MASLSLWFISFLGLLLGHGVLQLQASHHVYKAQTSHFVPHDYQQPYRTSYHFQPPKNWM- 60
           MAS SL FISFL  LL HG L+LQASHHVY  Q S  + H Y+QPYRTSYHFQPPKNW+ 
Sbjct: 1   MASSSLCFISFLCFLLCHGALKLQASHHVYTPQPSRLLLHRYEQPYRTSYHFQPPKNWIN 60

Query: 61  --NGPMIFKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWVPHEYAIYPSQPSDINGCW 120
             NGPMI+KGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINW PH+YAIYPSQPSDINGCW
Sbjct: 61  DPNGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWKPHDYAIYPSQPSDINGCW 120

Query: 121 SGSATILPGAKPAILYTGINPKSQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPENHI 180
           SGSATILPG KPAILYTGINPK+QQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTP+NHI
Sbjct: 121 SGSATILPGDKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQNHI 180

Query: 181 NSSSFRDPTTAWLGPDRQWRVIIGSKVDARGLAIMYRSKDFVQWDQVDHPLHYADGMGMW 240
           NSSSFRDPTTAWLG D +WRVIIGSK+DARGLAIM++SKDFV+WDQVDHPLHYADG GMW
Sbjct: 181 NSSSFRDPTTAWLGHDGEWRVIIGSKIDARGLAIMFQSKDFVKWDQVDHPLHYADGTGMW 240

Query: 241 ECPDFFPVVKKGRTGIDTVVNGPHVKHVLKISLDDTKHDLYTIGTYKLDKDIYVPDKGSI 300
           ECPDFFPV K  R G+DT V+G HVKHVLK+SLDDTKHD YT+GTY L KDIYVPDKGSI
Sbjct: 241 ECPDFFPVAKNSRAGVDTSVSGQHVKHVLKVSLDDTKHDQYTVGTYDLKKDIYVPDKGSI 300

Query: 301 ENYNGLRYDYGKYYASKTFFDSQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRTVWLD 360
           E Y GLRYDYGKYYASKTFFD QKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPR VWLD
Sbjct: 301 EGYTGLRYDYGKYYASKTFFDGQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRAVWLD 360

Query: 361 ASGKQLIQWPIVEIHKLRKDKVKLTNKVLKKGSVVEVKGVTAVQADVEVSFKVKNFNKAE 420
           ASGKQLIQWPIVEI KLRKDKVKLTNKVLKKGS++ VKGVTA QADV+VSFKVK+FNKA+
Sbjct: 361 ASGKQLIQWPIVEIQKLRKDKVKLTNKVLKKGSIIAVKGVTAAQADVDVSFKVKSFNKAQ 420

Query: 421 VLKPEWKDPQLLCSQKGTAADGGVGPFGLLVLASKDLKEYTSVLFRIFKHYNKYVVLLCS 480
            LK EWKDPQL+CSQ G AA GGVGPFGLLVLAS +L+EYTSV FRIFK  +KYVVL+CS
Sbjct: 421 PLKAEWKDPQLVCSQMGAAAKGGVGPFGLLVLASNNLEEYTSVFFRIFKRQDKYVVLMCS 480

Query: 481 DQSRSSLNSNNDKTTYGAFLDVDPVSENLSLRSLIDHSIVESFGSKGKACITARVYPTLA 540
           DQSRSSLN+NNDKTTYGAFLDVDPV EN+SLRSLIDHSIVESFGSKGKACITARVYP LA
Sbjct: 481 DQSRSSLNANNDKTTYGAFLDVDPVRENISLRSLIDHSIVESFGSKGKACITARVYPRLA 540

Query: 541 IGDKVGLYAFNNGTEPVTITKLNAWSLNKAQIS 571
           IGDK GLYAFNNGTE VTITKL+AWS+ KAQIS
Sbjct: 541 IGDKAGLYAFNNGTESVTITKLSAWSMKKAQIS 573

BLAST of Spg013912 vs. NCBI nr
Match: KAG6575468.1 (Beta-fructofuranosidase, insoluble isoenzyme CWINV1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1027.3 bits (2655), Expect = 5.0e-296
Identity = 488/569 (85.76%), Postives = 521/569 (91.56%), Query Frame = 0

Query: 1   MASLSLWFISFLGLLLGHGVLQLQASHHVYKAQTSHFVPHDYQQPYRTSYHFQPPKNWMN 60
           MAS SL FISFL  LL HG L+LQASHHVY  Q S  + H ++QPYRTS HFQPPKNW+N
Sbjct: 1   MASSSLCFISFLCFLLCHGALKLQASHHVYTPQPSRLLLHHHEQPYRTSCHFQPPKNWIN 60

Query: 61  GPMIFKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWVPHEYAIYPSQPSDINGCWSGS 120
           GPMI+KGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINW PH+YAIYPSQPSDINGCWSGS
Sbjct: 61  GPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWKPHDYAIYPSQPSDINGCWSGS 120

Query: 121 ATILPGAKPAILYTGINPKSQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPENHINSS 180
           ATILPG KPAILYTGINPK+QQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTP+N INSS
Sbjct: 121 ATILPGDKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQNQINSS 180

Query: 181 SFRDPTTAWLGPDRQWRVIIGSKVDARGLAIMYRSKDFVQWDQVDHPLHYADGMGMWECP 240
           SFRDPTTAWLGPD +WRVIIGSK+DARGLAIM+RSKDFV+WDQVDHPLHYADGMGMWECP
Sbjct: 181 SFRDPTTAWLGPDGEWRVIIGSKIDARGLAIMFRSKDFVKWDQVDHPLHYADGMGMWECP 240

Query: 241 DFFPVVKKGRTGIDTVVNGPHVKHVLKISLDDTKHDLYTIGTYKLDKDIYVPDKGSIENY 300
           DFFPV K GR G+DT V+G HVKHVLK+SLDDTKHD YT+GTY L KDIYVPDKGSIE+Y
Sbjct: 241 DFFPVAKNGRAGVDTSVSGQHVKHVLKVSLDDTKHDQYTVGTYDLKKDIYVPDKGSIEDY 300

Query: 301 NGLRYDYGKYYASKTFFDSQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRTVWLDASG 360
            GLRYDYGKYYASKTFFD QKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRTVWLDASG
Sbjct: 301 RGLRYDYGKYYASKTFFDDQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRTVWLDASG 360

Query: 361 KQLIQWPIVEIHKLRKDKVKLTNKVLKKGSVVEVKGVTAVQADVEVSFKVKNFNKAEVLK 420
           KQLIQWPI+EI KLR DKVKL +KVLKKGS++ VKGVTA QADV+VSFKVK+FNKA+ LK
Sbjct: 361 KQLIQWPIMEIQKLRNDKVKLADKVLKKGSIIAVKGVTAAQADVDVSFKVKSFNKAQKLK 420

Query: 421 PEWKDPQLLCSQKGTAADGGVGPFGLLVLASKDLKEYTSVLFRIFKHYNKYVVLLCSDQS 480
            EWKDPQL+CSQ G AA GGVGPFGLLVLAS +L+EYTSV FRIFK  +KYVVL+CSDQS
Sbjct: 421 AEWKDPQLVCSQMGAAAKGGVGPFGLLVLASNNLEEYTSVFFRIFKRQDKYVVLMCSDQS 480

Query: 481 RSSLNSNNDKTTYGAFLDVDPVSENLSLRSLIDHSIVESFGSKGKACITARVYPTLAIGD 540
           RSSLN+NNDKTTYGAFLDVDPV +N+SLRSLIDHSIVESFGSKGKACITARVYP LAIGD
Sbjct: 481 RSSLNANNDKTTYGAFLDVDPVRKNISLRSLIDHSIVESFGSKGKACITARVYPRLAIGD 540

Query: 541 KVGLYAFNNGTEPVTITKLNAWSLNKAQI 570
           K GLYAFNNGTE VTITKL AWSL KAQI
Sbjct: 541 KAGLYAFNNGTESVTITKLRAWSLKKAQI 569

BLAST of Spg013912 vs. ExPASy Swiss-Prot
Match: Q43866 (Beta-fructofuranosidase, insoluble isoenzyme CWINV1 OS=Arabidopsis thaliana OX=3702 GN=CWINV1 PE=1 SV=1)

HSP 1 Score: 800.4 bits (2066), Expect = 1.3e-230
Identity = 391/581 (67.30%), Postives = 462/581 (79.52%), Query Frame = 0

Query: 2   ASLSLWFISFLGLLLGHGVLQLQASHHVYK--AQTSHFVPHDYQQPYRTSYHFQPPKNWM 61
           +++ LW +  L LL+G+ V+ L+ASHHVYK   Q+++       QPYRT +HFQPPKNWM
Sbjct: 7   SNIGLWLL--LTLLIGNYVVNLEASHHVYKRLTQSTNTKSPSVNQPYRTGFHFQPPKNWM 66

Query: 62  ---NGPMIFKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWVPHEYAIYPSQPSDINGC 121
              NGPMI+KGIYHLFYQ+NPKGAVWGNIVWAHSTSTDLINW PH  AI+PS P DINGC
Sbjct: 67  NDPNGPMIYKGIYHLFYQWNPKGAVWGNIVWAHSTSTDLINWDPHPPAIFPSAPFDINGC 126

Query: 122 WSGSATILPGAKPAILYTGINPKSQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPENH 181
           WSGSATILP  KP ILYTGI+PK+QQVQN+A PKNLSDPYLREW KSP NPLMAP   N 
Sbjct: 127 WSGSATILPNGKPVILYTGIDPKNQQVQNIAEPKNLSDPYLREWKKSPLNPLMAPDAVNG 186

Query: 182 INSSSFRDPTTAWLGPDRQWRVIIGSKVDARGLAIMYRSKDFVQWDQVDHPLHYADGMGM 241
           IN+SSFRDPTTAWLG D++WRVIIGSK+  RGLAI Y SKDF++W++   PLHY DG GM
Sbjct: 187 INASSFRDPTTAWLGQDKKWRVIIGSKIHRRGLAITYTSKDFLKWEKSPEPLHYDDGSGM 246

Query: 242 WECPDFFPVVKKGRTGIDTVVNG-PH--VKHVLKISLDDTKHDLYTIGTYKLDKDIYVPD 301
           WECPDFFPV + G  G++T   G P+  +KHVLKISLDDTKHD YTIGTY   KD +VPD
Sbjct: 247 WECPDFFPVTRFGSNGVETSSFGEPNEILKHVLKISLDDTKHDYYTIGTYDRVKDKFVPD 306

Query: 302 KGSIENYNGLRYDYGKYYASKTFFDSQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRT 361
            G   +    RYDYGKYYASKTFFDS K RR+LWGW NESSSV+DD++KGWSG+Q IPR 
Sbjct: 307 NGFKMDGTAPRYDYGKYYASKTFFDSAKNRRILWGWTNESSSVEDDVEKGWSGIQTIPRK 366

Query: 362 VWLDASGKQLIQWPIVEIHKLRKDKVK-LTNKVLKKGSVVEVKGVTAVQADVEVSFKVKN 421
           +WLD SGKQLIQWP+ E+ +LR  +VK L NKVLK GS +EV GVTA QADVEV FKV++
Sbjct: 367 IWLDRSGKQLIQWPVREVERLRTKQVKNLRNKVLKSGSRLEVYGVTAAQADVEVLFKVRD 426

Query: 422 FNKAEVLKPEWKDPQLLCSQKGTAADGGVGPFGLLVLASKDLKEYTSVLFRIFK---HYN 481
             KA+V++P W DPQL+CS+   +   G+GPFGL+VLASK+L+EYTSV FRIFK   + N
Sbjct: 427 LEKADVIEPSWTDPQLICSKMNVSVKSGLGPFGLMVLASKNLEEYTSVYFRIFKARQNSN 486

Query: 482 KYVVLLCSDQSRSSLNSNNDKTTYGAFLDVDPVSENLSLRSLIDHSIVESFGSKGKACIT 541
           KYVVL+CSDQSRSSL  +NDKTTYGAF+D++P  + LSLR+LIDHS+VESFG KG+ACIT
Sbjct: 487 KYVVLMCSDQSRSSLKEDNDKTTYGAFVDINP-HQPLSLRALIDHSVVESFGGKGRACIT 546

Query: 542 ARVYPTLAIGDKVGLYAFNNGTEPVTITKLNAWSLNKAQIS 571
           +RVYP LAIG    L+AFN G + V +  LNAWS+N AQIS
Sbjct: 547 SRVYPKLAIGKSSHLFAFNYGYQSVDVLNLNAWSMNSAQIS 584

BLAST of Spg013912 vs. ExPASy Swiss-Prot
Match: Q43089 (Beta-fructofuranosidase, cell wall isozyme OS=Pisum sativum OX=3888 GN=BFRUCT1 PE=2 SV=1)

HSP 1 Score: 741.5 bits (1913), Expect = 7.1e-213
Identity = 355/553 (64.20%), Postives = 430/553 (77.76%), Query Frame = 0

Query: 1   MASLSLWFISFLGLLLGHGVLQLQASHHVY---KAQTSHFVPHDYQQPYRTSYHFQPPKN 60
           MA  S++ +S   L+    V+ ++A+HHVY   +  +SH       QPYRT+YHFQP KN
Sbjct: 1   MAISSIFLLSLFSLIY---VIPIEATHHVYQTLETLSSHHSSKSNHQPYRTAYHFQPLKN 60

Query: 61  WM---NGPMIFKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWVPHEYAIYPSQPSDIN 120
           W+   NGPM + G YHLFYQYNPKGAVWGNIVWAHS S DL+NW P ++AI+PSQPSDI 
Sbjct: 61  WINDPNGPMRYGGFYHLFYQYNPKGAVWGNIVWAHSVSKDLVNWTPLDHAIHPSQPSDIK 120

Query: 121 GCWSGSATILPGAKPAILYTGINPKSQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPE 180
           GCWSGSATILPG KPAILYTGI+P + QVQN+A+PKN+SDP LREW KSPKNPLM PT  
Sbjct: 121 GCWSGSATILPGGKPAILYTGIDPNNHQVQNIAIPKNMSDPLLREWKKSPKNPLMEPTIA 180

Query: 181 NHINSSSFRDPTTAWLGPDRQWRVIIGSKVDARGLAIMYRSKDFVQWDQVDHPLHYADGM 240
           N INSSSFRDPTT+WLG D  WRV+IGSK+D +G+AI+Y+SK+FV W +  HPLH A+G 
Sbjct: 181 NKINSSSFRDPTTSWLGKDGFWRVLIGSKIDTKGMAILYKSKNFVDWVEAKHPLHSAEGT 240

Query: 241 GMWECPDFFPVVKKG--RTGIDTVVNG-PHVKHVLKISLDDTKHDLYTIGTYKLDKDIYV 300
           GMWECPDF+PV+ K   RTG+DT  NG   V+HVLK+SLDDTKHD Y IG+Y + KD++V
Sbjct: 241 GMWECPDFYPVLDKNLLRTGVDTSRNGDDDVRHVLKVSLDDTKHDHYLIGSYDVVKDVFV 300

Query: 301 PDKGSIENYNGLRYDYGKYYASKTFFDSQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIP 360
           P+ G  +N   LRYDYGKYYASKTFFD  K RR+L GWVNESSSV DD+KKGWSG+  IP
Sbjct: 301 PENGFEDNGFVLRYDYGKYYASKTFFDDGKNRRILLGWVNESSSVADDVKKGWSGIHTIP 360

Query: 361 RTVWLDASGKQLIQWPIVEIHKLRKDKVKLTNKVLKKGSVVEVKGVTAVQADVEVSFKVK 420
           R +WL  SGKQL+QWP+ EI  LR + V    KV+K G  + + GV +VQADVE+SF++ 
Sbjct: 361 REIWLHESGKQLVQWPVKEIENLRMNPVNWPTKVIKGGERISITGVDSVQADVEISFEIS 420

Query: 421 NFNKAEVLKPEWKDPQLLCSQKGTAADGGVGPFGLLVLASKDLKEYTSVLFRIFKHYNKY 480
           +  K E L+ +W DPQLLCSQKG    GGVGPFGLLV AS+ LKEYT+V FRIFK+ +K 
Sbjct: 421 DLGKVESLR-KWIDPQLLCSQKGAGVKGGVGPFGLLVFASQGLKEYTAVFFRIFKYQDKN 480

Query: 481 VVLLCSDQSRSSLNSNNDKTTYGAFLDVDPVSENLSLRSLIDHSIVESFGSKGKACITAR 540
           +VL+CSDQSRSSLN +ND T+YG F+DVDP+ E LSLR+LIDHS+VESFG +G+AC+TAR
Sbjct: 481 LVLMCSDQSRSSLNKDNDMTSYGTFVDVDPLHEKLSLRTLIDHSVVESFGGEGRACVTAR 540

Query: 541 VYPTLAIGDKVGL 545
           VYPTLAI DK  L
Sbjct: 541 VYPTLAIHDKAML 549

BLAST of Spg013912 vs. ExPASy Swiss-Prot
Match: Q70XE6 (Fructan 6-exohydrolase OS=Beta vulgaris OX=161934 GN=6-FEH PE=1 SV=1)

HSP 1 Score: 726.5 bits (1874), Expect = 2.4e-208
Identity = 358/576 (62.15%), Postives = 437/576 (75.87%), Query Frame = 0

Query: 9   ISFLGLLLGHGVLQL---QASHHVYKAQTSHFVPHDYQ-----QPYRTSYHFQPPKNWM- 68
           +S   +LL HG++ +   QA HH +       + +D+Q      PYRT+YHFQ PKNWM 
Sbjct: 11  LSISMMLLSHGMIIIAKDQAIHH-HDDDHDDMLINDHQMINDDDPYRTAYHFQSPKNWMN 70

Query: 69  --NGPMIFKGIYHLFYQYNPKGAVW-GNIVWAHSTSTDLINWVPHEYAIYPSQPSDINGC 128
             NGPMI+KGIYHLFYQY P   VW   IVW HSTSTDLINW     A+ PS+P DINGC
Sbjct: 71  DPNGPMIYKGIYHLFYQYYPYDPVWHTEIVWGHSTSTDLINWTQQPIALSPSEPYDINGC 130

Query: 129 WSGSATILPGAKPAILYTGINPKSQQVQNLAVPKNLSDPYLREWVKSPKNPLMA--PTPE 188
           WSGS TILP  KP ILYTGIN K+ QVQNLA+PKNLSDPYL+EW+K P+NPLMA  PT  
Sbjct: 131 WSGSITILPQNKPVILYTGINNKNYQVQNLALPKNLSDPYLKEWIKLPQNPLMAGTPTNN 190

Query: 189 NHINSSSFRDPTTAWLGPDRQWRVIIGSKVDARGLAIMYRSKDFVQWDQVDHPLHYADGM 248
           N+IN+SSFRDP+TAW   D +WRVI+G++   RGLA+++ S DFV+W+   +PLH  +G 
Sbjct: 191 NNINASSFRDPSTAWQLSDGKWRVIVGTQQGKRGLAVLFTSDDFVKWNNTGNPLHSTEGN 250

Query: 249 GMWECPDFFPVVKKGRTGIDTVVNGPHVKHVLKISLDDTKHDLYTIGTYKLDKDIYVPDK 308
           G+WECPDFFPV      G DT + G  VKHVLK+SL DT+++ YTIG Y ++KDIYVPD+
Sbjct: 251 GIWECPDFFPVYVGKSLGADTSIIGDDVKHVLKLSLFDTQYEYYTIGRYDIEKDIYVPDE 310

Query: 309 GSIENYNGLRYDYGKYYASKTFFDSQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRTV 368
           GSIE+  GLRYDYGK+YASK+FFD +  RR+LWGWVNESS   DDIKKGWSGVQAIPRTV
Sbjct: 311 GSIESDLGLRYDYGKFYASKSFFDDETNRRILWGWVNESSIQADDIKKGWSGVQAIPRTV 370

Query: 369 WLDASGKQLIQWPIVEIHKLRKDKVKLTNKVLKKGSVVEVKGVTAVQADVEVSFKVKNFN 428
            LD SGKQL+QWP+ E+  LR++ V+L ++V+K GS+VE+  +TA QADVE+SFK+   N
Sbjct: 371 VLDKSGKQLVQWPLAEVDMLRENDVELPSQVIKGGSLVEISQITASQADVEISFKIPESN 430

Query: 429 KAEVLKPEWKDPQLLCSQKGTAADGGVGPFGLLVLASKDLKEYTSVLFRIFKHYNKYVVL 488
             E L     +PQ+LCSQKG +  G  GPFGLL LAS  L+EYT+V FRIFK  NKYVVL
Sbjct: 431 YVEELDSTCTNPQILCSQKGASIKGRFGPFGLLTLASMGLEEYTAVFFRIFKGPNKYVVL 490

Query: 489 LCSDQSRSSLNSNNDKTTYGAFLDVDPVSENLSLRSLIDHSIVESFGSKGKACITARVYP 548
           +CSDQ+RSSLN   DK ++G F+DVDP++E+LSLR LIDHSIVESF +KGK+CITARVYP
Sbjct: 491 MCSDQTRSSLNPTTDKLSFGIFVDVDPINEDLSLRILIDHSIVESFSAKGKSCITARVYP 550

Query: 549 TLAIGDKVGLYAFNNGTEPVTITKLNAWSLNKAQIS 571
           T+AI DK  LY FNNGTE V ITKL+AWS+ KAQI+
Sbjct: 551 TMAINDKAKLYVFNNGTEDVKITKLSAWSMKKAQIN 585

BLAST of Spg013912 vs. ExPASy Swiss-Prot
Match: Q67XZ3 (Beta-fructofuranosidase, insoluble isoenzyme CWINV3 OS=Arabidopsis thaliana OX=3702 GN=CWINV3 PE=1 SV=2)

HSP 1 Score: 714.1 bits (1842), Expect = 1.2e-204
Identity = 348/571 (60.95%), Postives = 413/571 (72.33%), Query Frame = 0

Query: 12  LGLLLGHGVLQLQASHHVYKAQTSHFVPHDYQQPYRTSYHFQPPKNWM---NGPMIFKGI 71
           L + L + +  L+AS H            D  QPYRT YHFQP KNWM   NGPMI+KGI
Sbjct: 15  LSMFLANFITDLEASSH-----------QDLNQPYRTGYHFQPLKNWMNDPNGPMIYKGI 74

Query: 72  YHLFYQYNPKGAVWG-NIVWAHSTSTDLINWVPHEYAIYPSQPSDINGCWSGSATILPGA 131
           YHLFYQYNP GAVW   IVW HSTS DL+NW+    A  PSQPSDINGCWSGS TILP  
Sbjct: 75  YHLFYQYNPYGAVWDVRIVWGHSTSVDLVNWISQPPAFNPSQPSDINGCWSGSVTILPNG 134

Query: 132 KPAILYTGINPKSQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPENHINSSSFRDPTT 191
           KP ILYTGI+    QVQN+AVP N+SDPYLREW K P+NPLM     N IN   FRDPTT
Sbjct: 135 KPVILYTGIDQNKGQVQNVAVPVNISDPYLREWSKPPQNPLMTTNAVNGINPDRFRDPTT 194

Query: 192 AWLGPDRQWRVIIGSKV-DARGLAIMYRSKDFVQWDQVDHPLHYADGMGMWECPDFFPVV 251
           AWLG D +WRVI+GS   D RGLAI+Y+S+DF  W Q   PLHY D  GMWECPDFFPV 
Sbjct: 195 AWLGRDGEWRVIVGSSTDDRRGLAILYKSRDFFNWTQSMKPLHYEDLTGMWECPDFFPVS 254

Query: 252 KKGRTGIDTVVNGPH-VKHVLKISLDDTKHDLYTIGTYKLDKDIYVPDKGSIENYNGLRY 311
             G  G++T   G + +KHVLK+SL +T HD YTIG+Y  +KD+YVPD G ++N +  R 
Sbjct: 255 ITGSDGVETSSVGENGIKHVLKVSLIETLHDYYTIGSYDREKDVYVPDLGFVQNESAPRL 314

Query: 312 DYGKYYASKTFFDSQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRTVWLDASGKQLIQ 371
           DYGKYYASKTF+D  KKRR+LWGWVNESS   DDI+KGWSG+Q+ PR +WLD SGK+L+Q
Sbjct: 315 DYGKYYASKTFYDDVKKRRILWGWVNESSPAKDDIEKGWSGLQSFPRKIWLDESGKELLQ 374

Query: 372 WPIVEIHKLRKDKVKLTNKVLKKGSVVEVKGVTAVQADVEVSFKVKNFNKAEVLKPEWKD 431
           WPI EI  LR  +V    KVLK GS ++V GVTA QADVEVSFKVK   KA+V++P W D
Sbjct: 375 WPIEEIETLRGQQVNWQKKVLKAGSTLQVHGVTAAQADVEVSFKVKELEKADVIEPSWTD 434

Query: 432 PQLLCSQKGTAADGGVGPFGLLVLASKDLKEYTSVLFRIFKHYN------KYVVLLCSDQ 491
           PQ +CSQ   +   G+GPFGL+VLAS D++EYTSV FRIFK  +      KYVVL+CSDQ
Sbjct: 435 PQKICSQGDLSVMSGLGPFGLMVLASNDMEEYTSVYFRIFKSNDDTNKKTKYVVLMCSDQ 494

Query: 492 SRSSLNSNNDKTTYGAFLDVDPVSENLSLRSLIDHSIVESFGSKGKACITARVYPTLAIG 551
           SRSSLN  NDK+T+GAF+ +DP  + +SLR+LIDHSIVES+G  G+ CIT+RVYP LAIG
Sbjct: 495 SRSSLNDENDKSTFGAFVAIDPSHQTISLRTLIDHSIVESYGGGGRTCITSRVYPKLAIG 554

Query: 552 DKVGLYAFNNGTEPVTITKLNAWSLNKAQIS 571
           +   L+ FN GT+ V I  L+AWSL  AQI+
Sbjct: 555 ENANLFVFNKGTQSVDILTLSAWSLKSAQIN 574

BLAST of Spg013912 vs. ExPASy Swiss-Prot
Match: Q9LIB9 (Beta-fructofuranosidase, insoluble isoenzyme CWINV5 OS=Arabidopsis thaliana OX=3702 GN=CWINV5 PE=2 SV=2)

HSP 1 Score: 701.0 bits (1808), Expect = 1.1e-200
Identity = 341/542 (62.92%), Postives = 417/542 (76.94%), Query Frame = 0

Query: 38  VPHDYQ-QPYRTSYHFQPPKNWM---NGPMIFKGIYHLFYQYNPKGAVW--GNIVWAHST 97
           VP++ + QPYRT YHFQPPKNWM   NGPMI+KGIYHLFYQ+N  GAV      VW H+T
Sbjct: 33  VPNNIKNQPYRTGYHFQPPKNWMNDPNGPMIYKGIYHLFYQWNQNGAVMDVNKTVWGHAT 92

Query: 98  STDLINWVPHEYAIYPSQPSDINGCWSGSATILPGAKPAILYTGINPKSQQVQNLAVPKN 157
           STDLINW+    AI PS+PSDINGCWSGS TILP  KP ILYTG +  ++QVQNL  PKN
Sbjct: 93  STDLINWITLSPAIRPSRPSDINGCWSGSVTILPNGKPVILYTGNDRYNRQVQNLVKPKN 152

Query: 158 LSDPYLREWVKSPKNPLMAPTPENHINSSSFRDPTTAWLGPDRQWRVIIGSKVDARGLAI 217
           L+DPYLR W KSP+NPL+ P+P NHINSS+FRDPTTAW G D +WR+  GS+   RGLAI
Sbjct: 153 LTDPYLRHWTKSPENPLVTPSPVNHINSSAFRDPTTAWFGRDGRWRITTGSQEGRRGLAI 212

Query: 218 MYRSKDFVQWDQVDHPLHYADGMGMWECPDFFPVVKKGRTGIDT-VVNGPHVKHVLKISL 277
           ++ SKDFV W Q   PLHY DG GMWECPDFFPV +    G+DT   +GP VKHVLK+SL
Sbjct: 213 LHTSKDFVIWKQSPKPLHYHDGTGMWECPDFFPVARTDSRGLDTSFSSGPMVKHVLKVSL 272

Query: 278 DDTKHDLYTIGTYKLDKDIYVPDKGSIENYNGLRYDYGKYYASKTFFDSQKKRRVLWGWV 337
            DT +D YTIGTY   +D+YVPDKG +++    RYDYGK+YASKTF+DS  +RR+LWGWV
Sbjct: 273 TDTFNDYYTIGTYDEVRDVYVPDKGFVQDETAPRYDYGKFYASKTFYDSVNQRRILWGWV 332

Query: 338 NESSSVDDDIKKGWSGVQAIPRTVWLDASGKQLIQWPIVEIHKLRKDKVKLTNKVLKKGS 397
           NESS   D+IKKGW+G+QAIPR VWLD SGK+L+QWP+ EI +LR  +VK  NK+LK GS
Sbjct: 333 NESSPEKDNIKKGWAGLQAIPRKVWLDESGKRLVQWPVKEIERLRTTQVKWGNKLLKGGS 392

Query: 398 VVEVKGVTAVQADVEVSFKVKNFN--KAEVLKPEWKDPQLLCSQKGTAADGGVGPFGLLV 457
           V+EV GVTA QADVEV FKV  F+  KA+V++P W DPQL+CSQ+   A  G+GPFGL+V
Sbjct: 393 VMEVHGVTAPQADVEVFFKVSGFDLEKADVIEPGWTDPQLICSQRN--ASSGLGPFGLMV 452

Query: 458 LASKDLKEYTSVLFRIFK---HYNKYVVLLCSDQSRSSLNSNNDKTTYGAFLDVDPVSEN 517
           LASK+++EYTSV  RIF+   +  ++VV++CSDQS SSL   NDKTTYGAFLD+ P  + 
Sbjct: 453 LASKNMEEYTSVNIRIFRAGENSKEHVVVMCSDQSTSSLEKGNDKTTYGAFLDISPY-QP 512

Query: 518 LSLRSLIDHSIVESFGSKGKACITARVYPTLAIGDKVGLYAFNNGTEPVTITKLNAWSLN 568
           +SLR+LID SIVESFG KGK CIT+RVYP LAIG++  L+AFN G++ V +  L+AWS+ 
Sbjct: 513 ISLRTLIDKSIVESFGGKGKTCITSRVYPKLAIGERTHLFAFNKGSQNVNVLSLSAWSMK 571

BLAST of Spg013912 vs. ExPASy TrEMBL
Match: A0A6J1GPU7 (beta-fructofuranosidase, insoluble isoenzyme CWINV1-like OS=Cucurbita moschata OX=3662 GN=LOC111456399 PE=3 SV=1)

HSP 1 Score: 1027.7 bits (2656), Expect = 1.8e-296
Identity = 491/573 (85.69%), Postives = 522/573 (91.10%), Query Frame = 0

Query: 1   MASLSLWFISFLGLLLGHGVLQLQASHHVYKAQTSHFVPHDYQQPYRTSYHFQPPKNWM- 60
           MAS SL FISFL  LL HG L+LQASHHVY  Q S  + H Y+QPYRTSYHFQPPKNW+ 
Sbjct: 1   MASSSLCFISFLCFLLCHGALKLQASHHVYTPQPSRLLLHRYEQPYRTSYHFQPPKNWIN 60

Query: 61  --NGPMIFKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWVPHEYAIYPSQPSDINGCW 120
             NGPMI+KGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINW PH+YAIYPSQPSDINGCW
Sbjct: 61  DPNGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWKPHDYAIYPSQPSDINGCW 120

Query: 121 SGSATILPGAKPAILYTGINPKSQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPENHI 180
           SGSATILPG KPAILYTGINPK+QQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTP+NHI
Sbjct: 121 SGSATILPGDKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQNHI 180

Query: 181 NSSSFRDPTTAWLGPDRQWRVIIGSKVDARGLAIMYRSKDFVQWDQVDHPLHYADGMGMW 240
           NSSSFRDPTTAWLG D +WRVIIGSK+DARGLAIM++SKDFV+WDQVDHPLHYADG GMW
Sbjct: 181 NSSSFRDPTTAWLGHDGEWRVIIGSKIDARGLAIMFQSKDFVKWDQVDHPLHYADGTGMW 240

Query: 241 ECPDFFPVVKKGRTGIDTVVNGPHVKHVLKISLDDTKHDLYTIGTYKLDKDIYVPDKGSI 300
           ECPDFFPV K  R G+DT V+G HVKHVLK+SLDDTKHD YT+GTY L KDIYVPDKGSI
Sbjct: 241 ECPDFFPVAKNSRAGVDTSVSGQHVKHVLKVSLDDTKHDQYTVGTYDLKKDIYVPDKGSI 300

Query: 301 ENYNGLRYDYGKYYASKTFFDSQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRTVWLD 360
           E Y GLRYDYGKYYASKTFFD QKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPR VWLD
Sbjct: 301 EGYTGLRYDYGKYYASKTFFDGQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRAVWLD 360

Query: 361 ASGKQLIQWPIVEIHKLRKDKVKLTNKVLKKGSVVEVKGVTAVQADVEVSFKVKNFNKAE 420
           ASGKQLIQWPIVEI KLRKDKVKLTNKVLKKGS++ VKGVTA QADV+VSFKVK+FNKA+
Sbjct: 361 ASGKQLIQWPIVEIQKLRKDKVKLTNKVLKKGSIIAVKGVTAAQADVDVSFKVKSFNKAQ 420

Query: 421 VLKPEWKDPQLLCSQKGTAADGGVGPFGLLVLASKDLKEYTSVLFRIFKHYNKYVVLLCS 480
            LK EWKDPQL+CSQ G AA GGVGPFGLLVLAS +L+EYTSV FRIFK  +KYVVL+CS
Sbjct: 421 PLKAEWKDPQLVCSQMGAAAKGGVGPFGLLVLASNNLEEYTSVFFRIFKRQDKYVVLMCS 480

Query: 481 DQSRSSLNSNNDKTTYGAFLDVDPVSENLSLRSLIDHSIVESFGSKGKACITARVYPTLA 540
           DQSRSSLN+NNDKTTYGAFLDVDPV EN+SLRSLIDHSIVESFGSKGKACITARVYP LA
Sbjct: 481 DQSRSSLNANNDKTTYGAFLDVDPVRENISLRSLIDHSIVESFGSKGKACITARVYPRLA 540

Query: 541 IGDKVGLYAFNNGTEPVTITKLNAWSLNKAQIS 571
           IGDK GLYAFNNGTE VTITKL+AWS+ KAQIS
Sbjct: 541 IGDKAGLYAFNNGTESVTITKLSAWSMKKAQIS 573

BLAST of Spg013912 vs. ExPASy TrEMBL
Match: A0A6J1GPY1 (beta-fructofuranosidase, insoluble isoenzyme CWINV1-like OS=Cucurbita moschata OX=3662 GN=LOC111456400 PE=3 SV=1)

HSP 1 Score: 1021.5 bits (2640), Expect = 1.3e-294
Identity = 487/572 (85.14%), Postives = 522/572 (91.26%), Query Frame = 0

Query: 1   MASLSLWFISFLGLLLGHGVLQLQASHHVYKAQTSHFVPHDYQQPYRTSYHFQPPKNWM- 60
           MAS SL FISFL  LL HG L+LQASHHVY  Q S  + H ++QPYRTS HFQPPKNW+ 
Sbjct: 1   MASSSLCFISFLCFLLCHGALKLQASHHVYTPQPSRLLLHHHEQPYRTSCHFQPPKNWIN 60

Query: 61  --NGPMIFKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWVPHEYAIYPSQPSDINGCW 120
             NGPMI+KGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINW PH+YAIYPSQPSDINGCW
Sbjct: 61  DPNGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWKPHDYAIYPSQPSDINGCW 120

Query: 121 SGSATILPGAKPAILYTGINPKSQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPENHI 180
           SGSATILPG KPAILYTGINPK+QQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTP+NHI
Sbjct: 121 SGSATILPGDKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQNHI 180

Query: 181 NSSSFRDPTTAWLGPDRQWRVIIGSKVDARGLAIMYRSKDFVQWDQVDHPLHYADGMGMW 240
           NSSSFRDPTTAWLGPD +WRVIIGSKVD RGLAIM+RSKDFV+WDQVDHPLHYADGMGMW
Sbjct: 181 NSSSFRDPTTAWLGPDGEWRVIIGSKVDDRGLAIMFRSKDFVKWDQVDHPLHYADGMGMW 240

Query: 241 ECPDFFPVVKKGRTGIDTVVNGPHVKHVLKISLDDTKHDLYTIGTYKLDKDIYVPDKGSI 300
           ECPDFFPV K GR G+DT V+G HVKHVLK+SLDDTKHD YT+GTY L KDIYVPDKGSI
Sbjct: 241 ECPDFFPVAKNGRAGVDTSVSGQHVKHVLKVSLDDTKHDQYTVGTYDLKKDIYVPDKGSI 300

Query: 301 ENYNGLRYDYGKYYASKTFFDSQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRTVWLD 360
           ++Y+GLRYDYGKYYASKTFFD QKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRTVWLD
Sbjct: 301 QDYSGLRYDYGKYYASKTFFDGQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRTVWLD 360

Query: 361 ASGKQLIQWPIVEIHKLRKDKVKLTNKVLKKGSVVEVKGVTAVQADVEVSFKVKNFNKAE 420
           ASGKQLIQWPI EI KLRKDK+KLTNKVL+KGS++ VKGVTA QADV+VSFKVK+FNKA 
Sbjct: 361 ASGKQLIQWPIAEIQKLRKDKIKLTNKVLEKGSIIAVKGVTAAQADVDVSFKVKSFNKAH 420

Query: 421 VLKPEWKDPQLLCSQKGTAADGGVGPFGLLVLASKDLKEYTSVLFRIFKHYNKYVVLLCS 480
           +LK EWKDPQL+CSQ G AA GGVGPFGLLVLAS +L+EYTSV FRIFK  +KYVVL+CS
Sbjct: 421 LLKAEWKDPQLVCSQMGAAAKGGVGPFGLLVLASNNLEEYTSVFFRIFKRQDKYVVLMCS 480

Query: 481 DQSRSSLNSNNDKTTYGAFLDVDPVSENLSLRSLIDHSIVESFGSKGKACITARVYPTLA 540
           DQSRSSLN++NDKT YGAFLDVDPV +N+SLRSLIDHSIVESFGSKGKACITARVYP LA
Sbjct: 481 DQSRSSLNADNDKTMYGAFLDVDPVRKNVSLRSLIDHSIVESFGSKGKACITARVYPRLA 540

Query: 541 IGDKVGLYAFNNGTEPVTITKLNAWSLNKAQI 570
           IGDK GLYAFNNGTE VTITKL AWSL KAQI
Sbjct: 541 IGDKAGLYAFNNGTESVTITKLRAWSLKKAQI 572

BLAST of Spg013912 vs. ExPASy TrEMBL
Match: A0A6J1DM23 (beta-fructofuranosidase, insoluble isoenzyme CWINV1-like OS=Momordica charantia OX=3673 GN=LOC111021302 PE=3 SV=1)

HSP 1 Score: 1017.3 bits (2629), Expect = 2.5e-293
Identity = 479/573 (83.60%), Postives = 523/573 (91.27%), Query Frame = 0

Query: 1   MASLSLWFISFLGLLLGHGVLQLQASHHVYKAQTSHFVPHDYQQPYRTSYHFQPPKNWM- 60
           MAS SLWF SFL + L HGV +L+ASHHVY+AQTS  VP D+QQPYRTSYHFQPPKNWM 
Sbjct: 1   MASFSLWFFSFLSVFLCHGVFKLEASHHVYRAQTSASVPPDHQQPYRTSYHFQPPKNWMN 60

Query: 61  --NGPMIFKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWVPHEYAIYPSQPSDINGCW 120
             NGPMI+KGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWVPH++AI+PSQPSDINGCW
Sbjct: 61  DPNGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWVPHQHAIFPSQPSDINGCW 120

Query: 121 SGSATILPGAKPAILYTGINPKSQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPENHI 180
           SGSATILPG KPA+LYTGINP++ QVQNLAVPKNLSDP+L EWVKSPKNPLMAPTP+N+I
Sbjct: 121 SGSATILPGNKPAMLYTGINPQNHQVQNLAVPKNLSDPFLIEWVKSPKNPLMAPTPQNNI 180

Query: 181 NSSSFRDPTTAWLGPDRQWRVIIGSKVDARGLAIMYRSKDFVQWDQVDHPLHYADGMGMW 240
           NSSSFRDPTTAWLG DRQWRV+IGSK++ARGLAIMYRSKDF+QWDQV++PLHYAD  GMW
Sbjct: 181 NSSSFRDPTTAWLGRDRQWRVVIGSKINARGLAIMYRSKDFIQWDQVNNPLHYADDTGMW 240

Query: 241 ECPDFFPVVKKGRTGIDTVVNGPHVKHVLKISLDDTKHDLYTIGTYKLDKDIYVPDKGSI 300
           ECPDFFPV K  R G+DT VNGP VKHVLK+SLDDTKHD YT+GTY + KD+YVPDKGS+
Sbjct: 241 ECPDFFPVAKNSRVGVDTSVNGPRVKHVLKVSLDDTKHDHYTVGTYNVKKDVYVPDKGSV 300

Query: 301 ENYNGLRYDYGKYYASKTFFDSQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRTVWLD 360
           E Y GLRYDYGK YASKTFFDS+KKRRVLWGWVNESSSVDDD KKGWSGVQAIPR++WLD
Sbjct: 301 EGYAGLRYDYGKCYASKTFFDSEKKRRVLWGWVNESSSVDDDKKKGWSGVQAIPRSIWLD 360

Query: 361 ASGKQLIQWPIVEIHKLRKDKVKLTNKVLKKGSVVEVKGVTAVQADVEVSFKVKNFNKAE 420
           ASGKQL+QWPIVEI KLRKDKVKL NKVLKKGSV+EVKGVTA Q DVEVSFKVKNF KA+
Sbjct: 361 ASGKQLVQWPIVEIQKLRKDKVKLINKVLKKGSVIEVKGVTAAQVDVEVSFKVKNFKKAQ 420

Query: 421 VLKPEWKDPQLLCSQKGTAADGGVGPFGLLVLASKDLKEYTSVLFRIFKHYNKYVVLLCS 480
           VLKPEWKDPQLLC+QKG A + GVGPFGLL+LASKDLKEYTSV F++FK   KYVVL+CS
Sbjct: 421 VLKPEWKDPQLLCAQKGAAKESGVGPFGLLLLASKDLKEYTSVFFKVFKREGKYVVLMCS 480

Query: 481 DQSRSSLNSNNDKTTYGAFLDVDPVSENLSLRSLIDHSIVESFGSKGKACITARVYPTLA 540
           DQSRSSLNSNNDKTTYGAF+D+DPV ENLSLRSLIDHSIVESFG KGKACITARVYPTLA
Sbjct: 481 DQSRSSLNSNNDKTTYGAFVDMDPVRENLSLRSLIDHSIVESFGVKGKACITARVYPTLA 540

Query: 541 IGDKVGLYAFNNGTEPVTITKLNAWSLNKAQIS 571
           IG+ VGLYAFNN TE VTI+KL AWSLNKA I+
Sbjct: 541 IGENVGLYAFNNATEGVTISKLTAWSLNKAHIN 573

BLAST of Spg013912 vs. ExPASy TrEMBL
Match: A0A6J1JP62 (beta-fructofuranosidase, insoluble isoenzyme CWINV1-like OS=Cucurbita maxima OX=3661 GN=LOC111488605 PE=3 SV=1)

HSP 1 Score: 1010.4 bits (2611), Expect = 3.0e-291
Identity = 483/573 (84.29%), Postives = 515/573 (89.88%), Query Frame = 0

Query: 1   MASLSLWFISFLGLLLGHGVLQLQASHHVYKAQTSHFVPHDYQQPYRTSYHFQPPKNWM- 60
           MAS SL FIS L  LL HG L+LQASHHVY AQ S  + H Y+QPYRTSYHFQPPKNW+ 
Sbjct: 3   MASSSLCFISLLCFLLCHGALKLQASHHVYTAQASRLLLHHYEQPYRTSYHFQPPKNWIN 62

Query: 61  --NGPMIFKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWVPHEYAIYPSQPSDINGCW 120
             NGPMI+KGIYHLFYQYNPKGA WGNIVWAHSTSTDLINW PH++AIYPSQPSDINGCW
Sbjct: 63  DPNGPMIYKGIYHLFYQYNPKGATWGNIVWAHSTSTDLINWKPHDHAIYPSQPSDINGCW 122

Query: 121 SGSATILPGAKPAILYTGINPKSQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPENHI 180
           SGSATILPG KPAILYTGINPK+QQVQNLA+PKNLSDPYLREWVKSP NPLMAPTP+N I
Sbjct: 123 SGSATILPGEKPAILYTGINPKNQQVQNLALPKNLSDPYLREWVKSPNNPLMAPTPQNRI 182

Query: 181 NSSSFRDPTTAWLGPDRQWRVIIGSKVDARGLAIMYRSKDFVQWDQVDHPLHYADGMGMW 240
           NSSSFRDPTTAWLG D QWRVIIGSK+DARGLAIM+RSKDFV+WDQVDHPLHYADG GMW
Sbjct: 183 NSSSFRDPTTAWLGHDGQWRVIIGSKIDARGLAIMFRSKDFVRWDQVDHPLHYADGTGMW 242

Query: 241 ECPDFFPVVKKGRTGIDTVVNGPHVKHVLKISLDDTKHDLYTIGTYKLDKDIYVPDKGSI 300
           ECPDFFPV K GR GIDT VNG HVKHVLK+SLDDTKHD YT+GTY L KDIYVPDK S 
Sbjct: 243 ECPDFFPVAKNGRAGIDTSVNGGHVKHVLKVSLDDTKHDHYTVGTYDLKKDIYVPDKEST 302

Query: 301 ENYNGLRYDYGKYYASKTFFDSQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRTVWLD 360
           E Y GLRYDYGKYYASKTFFD QKKRRVLWGWVNESSSVDDD+KKGWSGVQAIPR VWLD
Sbjct: 303 EGYTGLRYDYGKYYASKTFFDGQKKRRVLWGWVNESSSVDDDMKKGWSGVQAIPRAVWLD 362

Query: 361 ASGKQLIQWPIVEIHKLRKDKVKLTNKVLKKGSVVEVKGVTAVQADVEVSFKVKNFNKAE 420
           ASGKQLIQWPI EI KLRKDK+KL NKV+KKGS++ VKGVTA QADVEVSFKVK+F KA+
Sbjct: 363 ASGKQLIQWPIAEIQKLRKDKIKLRNKVVKKGSIIAVKGVTAAQADVEVSFKVKSFKKAQ 422

Query: 421 VLKPEWKDPQLLCSQKGTAADGGVGPFGLLVLASKDLKEYTSVLFRIFKHYNKYVVLLCS 480
            LK EWKDPQL CS+ G AA GGVGPFGLLVLASKDL+EYTSV FR+FK  +KYVVL+CS
Sbjct: 423 RLKAEWKDPQLACSRMGAAAMGGVGPFGLLVLASKDLEEYTSVFFRVFKGQDKYVVLMCS 482

Query: 481 DQSRSSLNSNNDKTTYGAFLDVDPVSENLSLRSLIDHSIVESFGSKGKACITARVYPTLA 540
           DQSRSSLN+NNDKTTYGAFLDVDPV EN+SLRSLIDHSIVESFGSKGKACITARVYP LA
Sbjct: 483 DQSRSSLNANNDKTTYGAFLDVDPVRENISLRSLIDHSIVESFGSKGKACITARVYPKLA 542

Query: 541 IGDKVGLYAFNNGTEPVTITKLNAWSLNKAQIS 571
           +GDK GLYAFNNGTE VTITKL+AWS+ KAQIS
Sbjct: 543 MGDKAGLYAFNNGTERVTITKLSAWSMKKAQIS 575

BLAST of Spg013912 vs. ExPASy TrEMBL
Match: A0A2I4JRK2 (Cell wall invertase 2 OS=Cucumis sativus OX=3659 PE=2 SV=1)

HSP 1 Score: 984.6 bits (2544), Expect = 1.8e-283
Identity = 473/581 (81.41%), Postives = 516/581 (88.81%), Query Frame = 0

Query: 3   SLSLWFIS----FLGLLLGHGVLQLQASHHVYKA--QTSHFVPHDY-QQPYRTSYHFQPP 62
           S SL F++     + LL GH  LQLQA HHVY+A  +TSHFV H + QQPYRTSYHFQPP
Sbjct: 5   SSSLCFLNNIFLLIFLLFGHEFLQLQALHHVYRASHRTSHFVLHQHQQQPYRTSYHFQPP 64

Query: 63  KNWM---NGPMIFKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWVPHEYAIYPSQPSD 122
           KNW+   NGPMI+KGIYH FYQYNPKGAVWGNIVWAHSTSTDLINW PH++AI+PSQPSD
Sbjct: 65  KNWINDPNGPMIYKGIYHFFYQYNPKGAVWGNIVWAHSTSTDLINWEPHDHAIFPSQPSD 124

Query: 123 INGCWSGSATILPGAKPAILYTGINPKSQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPT 182
           INGCWSGSATILPG KPAILYTGINPK+QQVQNLAVPKNLSDPYLREWVKSPKNPLMAPT
Sbjct: 125 INGCWSGSATILPGEKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPT 184

Query: 183 PENHINSSSFRDPTTAWLGPDRQWRVIIGSKVDARGLAIMYRSKDFVQWDQVDHPLHYAD 242
           P+NHIN SSFRDPTTAWLG D +WRVIIGSK+DARGLAIMYRSKDFV+W+QVDHPL Y D
Sbjct: 185 PQNHINVSSFRDPTTAWLGRDGEWRVIIGSKIDARGLAIMYRSKDFVRWNQVDHPLRYKD 244

Query: 243 GMGMWECPDFFPVVKKGRTGIDTVVNGPHVKHVLKISLDDTKHDLYTIGTYKLDKDIYVP 302
            MGMWECPDFFPV K GRTG++  V+G HVKH+LK+SLDDTKHD+YT+GTY   KDIY+P
Sbjct: 245 DMGMWECPDFFPVAKTGRTGVEMKVSGSHVKHILKVSLDDTKHDVYTVGTYNWKKDIYIP 304

Query: 303 DKGSIENYNGLRYDYGKYYASKTFFDSQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPR 362
           +KGSIE YNGLRYDYGKYYASKTFFDSQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPR
Sbjct: 305 NKGSIEGYNGLRYDYGKYYASKTFFDSQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPR 364

Query: 363 TVWLDASGKQLIQWPIVEIHKLRKDKVKLTNKVLKKGSVVEVKGVTAVQADVEVSFKVKN 422
            +WLDASGKQLIQWPIVEI KLR +KVKLTN+VLKKGS +EVKGVTA QADVE+SFKV+N
Sbjct: 365 AIWLDASGKQLIQWPIVEIQKLRNNKVKLTNEVLKKGSTIEVKGVTAAQADVEISFKVEN 424

Query: 423 FNKAEVLKPEWKDPQLLCSQKGTAA---DGGVGPFGLLVLASKDLKEYTSVLFRIFKHYN 482
             KAE+LKPEWKDPQLLCSQKGT++    G VGPFGLLVLAS DLKEYTSV F +FK  +
Sbjct: 425 LKKAEILKPEWKDPQLLCSQKGTSSTTEGGSVGPFGLLVLASNDLKEYTSVFFTVFKKPH 484

Query: 483 KYVVLLCSDQSRSSLNSNNDKTTYGAFLDVDPVSENLSLRSLIDHSIVESFGSKGKACIT 542
           KYVVL+CSDQSRSSL+SNNDKTTYGAFLDVDP  EN+SLRSLIDHSIVESFG KGK CIT
Sbjct: 485 KYVVLMCSDQSRSSLHSNNDKTTYGAFLDVDPTRENISLRSLIDHSIVESFGIKGKGCIT 544

Query: 543 ARVYPTLAIGDKVGLYAFNNGTEPVTITKLNAWSLNKAQIS 571
           ARVYPTLAIGD V LYAFNNGT+ VTITKL AWS+ KA I+
Sbjct: 545 ARVYPTLAIGDNVSLYAFNNGTDSVTITKLTAWSMKKAHIN 585

BLAST of Spg013912 vs. TAIR 10
Match: AT3G13790.2 (Glycosyl hydrolases family 32 protein )

HSP 1 Score: 805.8 bits (2080), Expect = 2.2e-233
Identity = 391/578 (67.65%), Postives = 462/578 (79.93%), Query Frame = 0

Query: 2   ASLSLWFISFLGLLLGHGVLQLQASHHVYK--AQTSHFVPHDYQQPYRTSYHFQPPKNWM 61
           +++ LW +  L LL+G+ V+ L+ASHHVYK   Q+++       QPYRT +HFQPPKNWM
Sbjct: 7   SNIGLWLL--LTLLIGNYVVNLEASHHVYKRLTQSTNTKSPSVNQPYRTGFHFQPPKNWM 66

Query: 62  NGPMIFKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWVPHEYAIYPSQPSDINGCWSG 121
           NGPMI+KGIYHLFYQ+NPKGAVWGNIVWAHSTSTDLINW PH  AI+PS P DINGCWSG
Sbjct: 67  NGPMIYKGIYHLFYQWNPKGAVWGNIVWAHSTSTDLINWDPHPPAIFPSAPFDINGCWSG 126

Query: 122 SATILPGAKPAILYTGINPKSQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPENHINS 181
           SATILP  KP ILYTGI+PK+QQVQN+A PKNLSDPYLREW KSP NPLMAP   N IN+
Sbjct: 127 SATILPNGKPVILYTGIDPKNQQVQNIAEPKNLSDPYLREWKKSPLNPLMAPDAVNGINA 186

Query: 182 SSFRDPTTAWLGPDRQWRVIIGSKVDARGLAIMYRSKDFVQWDQVDHPLHYADGMGMWEC 241
           SSFRDPTTAWLG D++WRVIIGSK+  RGLAI Y SKDF++W++   PLHY DG GMWEC
Sbjct: 187 SSFRDPTTAWLGQDKKWRVIIGSKIHRRGLAITYTSKDFLKWEKSPEPLHYDDGSGMWEC 246

Query: 242 PDFFPVVKKGRTGIDTVVNG-PH--VKHVLKISLDDTKHDLYTIGTYKLDKDIYVPDKGS 301
           PDFFPV + G  G++T   G P+  +KHVLKISLDDTKHD YTIGTY   KD +VPD G 
Sbjct: 247 PDFFPVTRFGSNGVETSSFGEPNEILKHVLKISLDDTKHDYYTIGTYDRVKDKFVPDNGF 306

Query: 302 IENYNGLRYDYGKYYASKTFFDSQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRTVWL 361
             +    RYDYGKYYASKTFFDS K RR+LWGW NESSSV+DD++KGWSG+Q IPR +WL
Sbjct: 307 KMDGTAPRYDYGKYYASKTFFDSAKNRRILWGWTNESSSVEDDVEKGWSGIQTIPRKIWL 366

Query: 362 DASGKQLIQWPIVEIHKLRKDKVK-LTNKVLKKGSVVEVKGVTAVQADVEVSFKVKNFNK 421
           D SGKQLIQWP+ E+ +LR  +VK L NKVLK GS +EV GVTA QADVEV FKV++  K
Sbjct: 367 DRSGKQLIQWPVREVERLRTKQVKNLRNKVLKSGSRLEVYGVTAAQADVEVLFKVRDLEK 426

Query: 422 AEVLKPEWKDPQLLCSQKGTAADGGVGPFGLLVLASKDLKEYTSVLFRIFK---HYNKYV 481
           A+V++P W DPQL+CS+   +   G+GPFGL+VLASK+L+EYTSV FRIFK   + NKYV
Sbjct: 427 ADVIEPSWTDPQLICSKMNVSVKSGLGPFGLMVLASKNLEEYTSVYFRIFKARQNSNKYV 486

Query: 482 VLLCSDQSRSSLNSNNDKTTYGAFLDVDPVSENLSLRSLIDHSIVESFGSKGKACITARV 541
           VL+CSDQSRSSL  +NDKTTYGAF+D++P  + LSLR+LIDHS+VESFG KG+ACIT+RV
Sbjct: 487 VLMCSDQSRSSLKEDNDKTTYGAFVDINP-HQPLSLRALIDHSVVESFGGKGRACITSRV 546

Query: 542 YPTLAIGDKVGLYAFNNGTEPVTITKLNAWSLNKAQIS 571
           YP LAIG    L+AFN G + V +  LNAWS+N AQIS
Sbjct: 547 YPKLAIGKSSHLFAFNYGYQSVDVLNLNAWSMNSAQIS 581

BLAST of Spg013912 vs. TAIR 10
Match: AT3G13790.1 (Glycosyl hydrolases family 32 protein )

HSP 1 Score: 800.4 bits (2066), Expect = 9.2e-232
Identity = 391/581 (67.30%), Postives = 462/581 (79.52%), Query Frame = 0

Query: 2   ASLSLWFISFLGLLLGHGVLQLQASHHVYK--AQTSHFVPHDYQQPYRTSYHFQPPKNWM 61
           +++ LW +  L LL+G+ V+ L+ASHHVYK   Q+++       QPYRT +HFQPPKNWM
Sbjct: 7   SNIGLWLL--LTLLIGNYVVNLEASHHVYKRLTQSTNTKSPSVNQPYRTGFHFQPPKNWM 66

Query: 62  ---NGPMIFKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWVPHEYAIYPSQPSDINGC 121
              NGPMI+KGIYHLFYQ+NPKGAVWGNIVWAHSTSTDLINW PH  AI+PS P DINGC
Sbjct: 67  NDPNGPMIYKGIYHLFYQWNPKGAVWGNIVWAHSTSTDLINWDPHPPAIFPSAPFDINGC 126

Query: 122 WSGSATILPGAKPAILYTGINPKSQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPENH 181
           WSGSATILP  KP ILYTGI+PK+QQVQN+A PKNLSDPYLREW KSP NPLMAP   N 
Sbjct: 127 WSGSATILPNGKPVILYTGIDPKNQQVQNIAEPKNLSDPYLREWKKSPLNPLMAPDAVNG 186

Query: 182 INSSSFRDPTTAWLGPDRQWRVIIGSKVDARGLAIMYRSKDFVQWDQVDHPLHYADGMGM 241
           IN+SSFRDPTTAWLG D++WRVIIGSK+  RGLAI Y SKDF++W++   PLHY DG GM
Sbjct: 187 INASSFRDPTTAWLGQDKKWRVIIGSKIHRRGLAITYTSKDFLKWEKSPEPLHYDDGSGM 246

Query: 242 WECPDFFPVVKKGRTGIDTVVNG-PH--VKHVLKISLDDTKHDLYTIGTYKLDKDIYVPD 301
           WECPDFFPV + G  G++T   G P+  +KHVLKISLDDTKHD YTIGTY   KD +VPD
Sbjct: 247 WECPDFFPVTRFGSNGVETSSFGEPNEILKHVLKISLDDTKHDYYTIGTYDRVKDKFVPD 306

Query: 302 KGSIENYNGLRYDYGKYYASKTFFDSQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRT 361
            G   +    RYDYGKYYASKTFFDS K RR+LWGW NESSSV+DD++KGWSG+Q IPR 
Sbjct: 307 NGFKMDGTAPRYDYGKYYASKTFFDSAKNRRILWGWTNESSSVEDDVEKGWSGIQTIPRK 366

Query: 362 VWLDASGKQLIQWPIVEIHKLRKDKVK-LTNKVLKKGSVVEVKGVTAVQADVEVSFKVKN 421
           +WLD SGKQLIQWP+ E+ +LR  +VK L NKVLK GS +EV GVTA QADVEV FKV++
Sbjct: 367 IWLDRSGKQLIQWPVREVERLRTKQVKNLRNKVLKSGSRLEVYGVTAAQADVEVLFKVRD 426

Query: 422 FNKAEVLKPEWKDPQLLCSQKGTAADGGVGPFGLLVLASKDLKEYTSVLFRIFK---HYN 481
             KA+V++P W DPQL+CS+   +   G+GPFGL+VLASK+L+EYTSV FRIFK   + N
Sbjct: 427 LEKADVIEPSWTDPQLICSKMNVSVKSGLGPFGLMVLASKNLEEYTSVYFRIFKARQNSN 486

Query: 482 KYVVLLCSDQSRSSLNSNNDKTTYGAFLDVDPVSENLSLRSLIDHSIVESFGSKGKACIT 541
           KYVVL+CSDQSRSSL  +NDKTTYGAF+D++P  + LSLR+LIDHS+VESFG KG+ACIT
Sbjct: 487 KYVVLMCSDQSRSSLKEDNDKTTYGAFVDINP-HQPLSLRALIDHSVVESFGGKGRACIT 546

Query: 542 ARVYPTLAIGDKVGLYAFNNGTEPVTITKLNAWSLNKAQIS 571
           +RVYP LAIG    L+AFN G + V +  LNAWS+N AQIS
Sbjct: 547 SRVYPKLAIGKSSHLFAFNYGYQSVDVLNLNAWSMNSAQIS 584

BLAST of Spg013912 vs. TAIR 10
Match: AT1G55120.1 (beta-fructofuranosidase 5 )

HSP 1 Score: 714.1 bits (1842), Expect = 8.7e-206
Identity = 348/571 (60.95%), Postives = 413/571 (72.33%), Query Frame = 0

Query: 12  LGLLLGHGVLQLQASHHVYKAQTSHFVPHDYQQPYRTSYHFQPPKNWM---NGPMIFKGI 71
           L + L + +  L+AS H            D  QPYRT YHFQP KNWM   NGPMI+KGI
Sbjct: 15  LSMFLANFITDLEASSH-----------QDLNQPYRTGYHFQPLKNWMNDPNGPMIYKGI 74

Query: 72  YHLFYQYNPKGAVWG-NIVWAHSTSTDLINWVPHEYAIYPSQPSDINGCWSGSATILPGA 131
           YHLFYQYNP GAVW   IVW HSTS DL+NW+    A  PSQPSDINGCWSGS TILP  
Sbjct: 75  YHLFYQYNPYGAVWDVRIVWGHSTSVDLVNWISQPPAFNPSQPSDINGCWSGSVTILPNG 134

Query: 132 KPAILYTGINPKSQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPENHINSSSFRDPTT 191
           KP ILYTGI+    QVQN+AVP N+SDPYLREW K P+NPLM     N IN   FRDPTT
Sbjct: 135 KPVILYTGIDQNKGQVQNVAVPVNISDPYLREWSKPPQNPLMTTNAVNGINPDRFRDPTT 194

Query: 192 AWLGPDRQWRVIIGSKV-DARGLAIMYRSKDFVQWDQVDHPLHYADGMGMWECPDFFPVV 251
           AWLG D +WRVI+GS   D RGLAI+Y+S+DF  W Q   PLHY D  GMWECPDFFPV 
Sbjct: 195 AWLGRDGEWRVIVGSSTDDRRGLAILYKSRDFFNWTQSMKPLHYEDLTGMWECPDFFPVS 254

Query: 252 KKGRTGIDTVVNGPH-VKHVLKISLDDTKHDLYTIGTYKLDKDIYVPDKGSIENYNGLRY 311
             G  G++T   G + +KHVLK+SL +T HD YTIG+Y  +KD+YVPD G ++N +  R 
Sbjct: 255 ITGSDGVETSSVGENGIKHVLKVSLIETLHDYYTIGSYDREKDVYVPDLGFVQNESAPRL 314

Query: 312 DYGKYYASKTFFDSQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRTVWLDASGKQLIQ 371
           DYGKYYASKTF+D  KKRR+LWGWVNESS   DDI+KGWSG+Q+ PR +WLD SGK+L+Q
Sbjct: 315 DYGKYYASKTFYDDVKKRRILWGWVNESSPAKDDIEKGWSGLQSFPRKIWLDESGKELLQ 374

Query: 372 WPIVEIHKLRKDKVKLTNKVLKKGSVVEVKGVTAVQADVEVSFKVKNFNKAEVLKPEWKD 431
           WPI EI  LR  +V    KVLK GS ++V GVTA QADVEVSFKVK   KA+V++P W D
Sbjct: 375 WPIEEIETLRGQQVNWQKKVLKAGSTLQVHGVTAAQADVEVSFKVKELEKADVIEPSWTD 434

Query: 432 PQLLCSQKGTAADGGVGPFGLLVLASKDLKEYTSVLFRIFKHYN------KYVVLLCSDQ 491
           PQ +CSQ   +   G+GPFGL+VLAS D++EYTSV FRIFK  +      KYVVL+CSDQ
Sbjct: 435 PQKICSQGDLSVMSGLGPFGLMVLASNDMEEYTSVYFRIFKSNDDTNKKTKYVVLMCSDQ 494

Query: 492 SRSSLNSNNDKTTYGAFLDVDPVSENLSLRSLIDHSIVESFGSKGKACITARVYPTLAIG 551
           SRSSLN  NDK+T+GAF+ +DP  + +SLR+LIDHSIVES+G  G+ CIT+RVYP LAIG
Sbjct: 495 SRSSLNDENDKSTFGAFVAIDPSHQTISLRTLIDHSIVESYGGGGRTCITSRVYPKLAIG 554

Query: 552 DKVGLYAFNNGTEPVTITKLNAWSLNKAQIS 571
           +   L+ FN GT+ V I  L+AWSL  AQI+
Sbjct: 555 ENANLFVFNKGTQSVDILTLSAWSLKSAQIN 574

BLAST of Spg013912 vs. TAIR 10
Match: AT3G13784.1 (cell wall invertase 5 )

HSP 1 Score: 706.4 bits (1822), Expect = 1.8e-203
Identity = 341/539 (63.27%), Postives = 417/539 (77.37%), Query Frame = 0

Query: 38  VPHDYQ-QPYRTSYHFQPPKNWMNGPMIFKGIYHLFYQYNPKGAVW--GNIVWAHSTSTD 97
           VP++ + QPYRT YHFQPPKNWMNGPMI+KGIYHLFYQ+N  GAV      VW H+TSTD
Sbjct: 33  VPNNIKNQPYRTGYHFQPPKNWMNGPMIYKGIYHLFYQWNQNGAVMDVNKTVWGHATSTD 92

Query: 98  LINWVPHEYAIYPSQPSDINGCWSGSATILPGAKPAILYTGINPKSQQVQNLAVPKNLSD 157
           LINW+    AI PS+PSDINGCWSGS TILP  KP ILYTG +  ++QVQNL  PKNL+D
Sbjct: 93  LINWITLSPAIRPSRPSDINGCWSGSVTILPNGKPVILYTGNDRYNRQVQNLVKPKNLTD 152

Query: 158 PYLREWVKSPKNPLMAPTPENHINSSSFRDPTTAWLGPDRQWRVIIGSKVDARGLAIMYR 217
           PYLR W KSP+NPL+ P+P NHINSS+FRDPTTAW G D +WR+  GS+   RGLAI++ 
Sbjct: 153 PYLRHWTKSPENPLVTPSPVNHINSSAFRDPTTAWFGRDGRWRITTGSQEGRRGLAILHT 212

Query: 218 SKDFVQWDQVDHPLHYADGMGMWECPDFFPVVKKGRTGIDT-VVNGPHVKHVLKISLDDT 277
           SKDFV W Q   PLHY DG GMWECPDFFPV +    G+DT   +GP VKHVLK+SL DT
Sbjct: 213 SKDFVIWKQSPKPLHYHDGTGMWECPDFFPVARTDSRGLDTSFSSGPMVKHVLKVSLTDT 272

Query: 278 KHDLYTIGTYKLDKDIYVPDKGSIENYNGLRYDYGKYYASKTFFDSQKKRRVLWGWVNES 337
            +D YTIGTY   +D+YVPDKG +++    RYDYGK+YASKTF+DS  +RR+LWGWVNES
Sbjct: 273 FNDYYTIGTYDEVRDVYVPDKGFVQDETAPRYDYGKFYASKTFYDSVNQRRILWGWVNES 332

Query: 338 SSVDDDIKKGWSGVQAIPRTVWLDASGKQLIQWPIVEIHKLRKDKVKLTNKVLKKGSVVE 397
           S   D+IKKGW+G+QAIPR VWLD SGK+L+QWP+ EI +LR  +VK  NK+LK GSV+E
Sbjct: 333 SPEKDNIKKGWAGLQAIPRKVWLDESGKRLVQWPVKEIERLRTTQVKWGNKLLKGGSVME 392

Query: 398 VKGVTAVQADVEVSFKVKNFN--KAEVLKPEWKDPQLLCSQKGTAADGGVGPFGLLVLAS 457
           V GVTA QADVEV FKV  F+  KA+V++P W DPQL+CSQ+   A  G+GPFGL+VLAS
Sbjct: 393 VHGVTAPQADVEVFFKVSGFDLEKADVIEPGWTDPQLICSQRN--ASSGLGPFGLMVLAS 452

Query: 458 KDLKEYTSVLFRIFK---HYNKYVVLLCSDQSRSSLNSNNDKTTYGAFLDVDPVSENLSL 517
           K+++EYTSV  RIF+   +  ++VV++CSDQS SSL   NDKTTYGAFLD+ P  + +SL
Sbjct: 453 KNMEEYTSVNIRIFRAGENSKEHVVVMCSDQSTSSLEKGNDKTTYGAFLDISPY-QPISL 512

Query: 518 RSLIDHSIVESFGSKGKACITARVYPTLAIGDKVGLYAFNNGTEPVTITKLNAWSLNKA 568
           R+LID SIVESFG KGK CIT+RVYP LAIG++  L+AFN G++ V +  L+AWS+  +
Sbjct: 513 RTLIDKSIVESFGGKGKTCITSRVYPKLAIGERTHLFAFNKGSQNVNVLSLSAWSMKSS 568

BLAST of Spg013912 vs. TAIR 10
Match: AT1G55120.3 (beta-fructofuranosidase 5 )

HSP 1 Score: 683.3 bits (1762), Expect = 1.6e-196
Identity = 327/525 (62.29%), Postives = 390/525 (74.29%), Query Frame = 0

Query: 55  PKNWMNGPMIFKGIYHLFYQYNPKGAVWG-NIVWAHSTSTDLINWVPHEYAIYPSQPSDI 114
           P   ++GPMI+KGIYHLFYQYNP GAVW   IVW HSTS DL+NW+    A  PSQPSDI
Sbjct: 35  PNVSVSGPMIYKGIYHLFYQYNPYGAVWDVRIVWGHSTSVDLVNWISQPPAFNPSQPSDI 94

Query: 115 NGCWSGSATILPGAKPAILYTGINPKSQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTP 174
           NGCWSGS TILP  KP ILYTGI+    QVQN+AVP N+SDPYLREW K P+NPLM    
Sbjct: 95  NGCWSGSVTILPNGKPVILYTGIDQNKGQVQNVAVPVNISDPYLREWSKPPQNPLMTTNA 154

Query: 175 ENHINSSSFRDPTTAWLGPDRQWRVIIGSKV-DARGLAIMYRSKDFVQWDQVDHPLHYAD 234
            N IN   FRDPTTAWLG D +WRVI+GS   D RGLAI+Y+S+DF  W Q   PLHY D
Sbjct: 155 VNGINPDRFRDPTTAWLGRDGEWRVIVGSSTDDRRGLAILYKSRDFFNWTQSMKPLHYED 214

Query: 235 GMGMWECPDFFPVVKKGRTGIDTVVNGPH-VKHVLKISLDDTKHDLYTIGTYKLDKDIYV 294
             GMWECPDFFPV   G  G++T   G + +KHVLK+SL +T HD YTIG+Y  +KD+YV
Sbjct: 215 LTGMWECPDFFPVSITGSDGVETSSVGENGIKHVLKVSLIETLHDYYTIGSYDREKDVYV 274

Query: 295 PDKGSIENYNGLRYDYGKYYASKTFFDSQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIP 354
           PD G ++N +  R DYGKYYASKTF+D  KKRR+LWGWVNESS   DDI+KGWSG+Q+ P
Sbjct: 275 PDLGFVQNESAPRLDYGKYYASKTFYDDVKKRRILWGWVNESSPAKDDIEKGWSGLQSFP 334

Query: 355 RTVWLDASGKQLIQWPIVEIHKLRKDKVKLTNKVLKKGSVVEVKGVTAVQADVEVSFKVK 414
           R +WLD SGK+L+QWPI EI  LR  +V    KVLK GS ++V GVTA QADVEVSFKVK
Sbjct: 335 RKIWLDESGKELLQWPIEEIETLRGQQVNWQKKVLKAGSTLQVHGVTAAQADVEVSFKVK 394

Query: 415 NFNKAEVLKPEWKDPQLLCSQKGTAADGGVGPFGLLVLASKDLKEYTSVLFRIFKHYN-- 474
              KA+V++P W DPQ +CSQ   +   G+GPFGL+VLAS D++EYTSV FRIFK  +  
Sbjct: 395 ELEKADVIEPSWTDPQKICSQGDLSVMSGLGPFGLMVLASNDMEEYTSVYFRIFKSNDDT 454

Query: 475 ----KYVVLLCSDQSRSSLNSNNDKTTYGAFLDVDPVSENLSLRSLIDHSIVESFGSKGK 534
               KYVVL+CSDQSRSSLN  NDK+T+GAF+ +DP  + +SLR+LIDHSIVES+G  G+
Sbjct: 455 NKKTKYVVLMCSDQSRSSLNDENDKSTFGAFVAIDPSHQTISLRTLIDHSIVESYGGGGR 514

Query: 535 ACITARVYPTLAIGDKVGLYAFNNGTEPVTITKLNAWSLNKAQIS 571
            CIT+RVYP LAIG+   L+ FN GT+ V I  L+AWSL  AQI+
Sbjct: 515 TCITSRVYPKLAIGENANLFVFNKGTQSVDILTLSAWSLKSAQIN 559

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG6575469.15.7e-30086.67Beta-fructofuranosidase, insoluble isoenzyme CWINV1, partial [Cucurbita argyrosp... [more]
XP_038898203.11.3e-29985.86beta-fructofuranosidase, insoluble isoenzyme CWINV1-like [Benincasa hispida][more]
XP_023547447.12.9e-29685.84beta-fructofuranosidase, insoluble isoenzyme CWINV1-like [Cucurbita pepo subsp. ... [more]
XP_022954011.13.8e-29685.69beta-fructofuranosidase, insoluble isoenzyme CWINV1-like [Cucurbita moschata] >X... [more]
KAG6575468.15.0e-29685.76Beta-fructofuranosidase, insoluble isoenzyme CWINV1, partial [Cucurbita argyrosp... [more]
Match NameE-valueIdentityDescription
Q438661.3e-23067.30Beta-fructofuranosidase, insoluble isoenzyme CWINV1 OS=Arabidopsis thaliana OX=3... [more]
Q430897.1e-21364.20Beta-fructofuranosidase, cell wall isozyme OS=Pisum sativum OX=3888 GN=BFRUCT1 P... [more]
Q70XE62.4e-20862.15Fructan 6-exohydrolase OS=Beta vulgaris OX=161934 GN=6-FEH PE=1 SV=1[more]
Q67XZ31.2e-20460.95Beta-fructofuranosidase, insoluble isoenzyme CWINV3 OS=Arabidopsis thaliana OX=3... [more]
Q9LIB91.1e-20062.92Beta-fructofuranosidase, insoluble isoenzyme CWINV5 OS=Arabidopsis thaliana OX=3... [more]
Match NameE-valueIdentityDescription
A0A6J1GPU71.8e-29685.69beta-fructofuranosidase, insoluble isoenzyme CWINV1-like OS=Cucurbita moschata O... [more]
A0A6J1GPY11.3e-29485.14beta-fructofuranosidase, insoluble isoenzyme CWINV1-like OS=Cucurbita moschata O... [more]
A0A6J1DM232.5e-29383.60beta-fructofuranosidase, insoluble isoenzyme CWINV1-like OS=Momordica charantia ... [more]
A0A6J1JP623.0e-29184.29beta-fructofuranosidase, insoluble isoenzyme CWINV1-like OS=Cucurbita maxima OX=... [more]
A0A2I4JRK21.8e-28381.41Cell wall invertase 2 OS=Cucumis sativus OX=3659 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
AT3G13790.22.2e-23367.65Glycosyl hydrolases family 32 protein [more]
AT3G13790.19.2e-23267.30Glycosyl hydrolases family 32 protein [more]
AT1G55120.18.7e-20660.95beta-fructofuranosidase 5 [more]
AT3G13784.11.8e-20363.27cell wall invertase 5 [more]
AT1G55120.31.6e-19662.29beta-fructofuranosidase 5 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001362Glycoside hydrolase, family 32SMARTSM00640glyco_32coord: 51..524
e-value: 2.7E-241
score: 817.6
IPR023296Glycosyl hydrolase, five-bladed beta-propellor domain superfamilyGENE3D2.115.10.20Glycosyl hydrolase domain; family 43coord: 55..378
e-value: 2.7E-112
score: 377.5
IPR023296Glycosyl hydrolase, five-bladed beta-propellor domain superfamilySUPERFAMILY75005Arabinanase/levansucrase/invertasecoord: 42..368
IPR013148Glycosyl hydrolase family 32, N-terminalPFAMPF00251Glyco_hydro_32Ncoord: 51..367
e-value: 3.6E-102
score: 342.0
NoneNo IPR availableGENE3D2.60.120.560coord: 379..564
e-value: 7.7E-82
score: 275.6
NoneNo IPR availablePANTHERPTHR31953BETA-FRUCTOFURANOSIDASE, INSOLUBLE ISOENZYME CWINV1-RELATEDcoord: 7..569
NoneNo IPR availablePANTHERPTHR31953:SF51CELL-WALL INVERTASEcoord: 7..569
NoneNo IPR availableCDDcd18624GH32_Fruct1-likecoord: 57..356
e-value: 7.63783E-162
score: 461.085
IPR013189Glycosyl hydrolase family 32, C-terminalPFAMPF08244Glyco_hydro_32Ccoord: 370..563
e-value: 1.8E-28
score: 99.6
IPR013320Concanavalin A-like lectin/glucanase domain superfamilySUPERFAMILY49899Concanavalin A-like lectins/glucanasescoord: 367..566

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg013912.1Spg013912.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds