Spg013516 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg013516 vs. NCBI nr
Match: KAG6594817.1 (Auxilin-like protein 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 2176.4 bits (5638), Expect = 0.0e+00
Identity = 1191/1455 (81.86%), Postives = 1282/1455 (88.11%), Query Frame = 0

Query: 1    MDNLSHSRLPNRGSTSVSKKICNGSNGGGPFVAQTIYDDVYGGPPKFGVSALSPRFEDYG 60
            MDNLSHSRLPNRGSTSVSKKICNGSNGGGPFVAQTIYDDVYGGPPKFGVSALSPRFEDYG
Sbjct: 1    MDNLSHSRLPNRGSTSVSKKICNGSNGGGPFVAQTIYDDVYGGPPKFGVSALSPRFEDYG 60

Query: 61   EIFGSFHALRASSIPILDLPAVDESEVFFDARSSAFDYTEVFGGFDGLDFAISYEELVGP 120
            EIFGSFHALR SSIPILDLPAVDESEVFFDARSSAFDY EVFGGFDGL+FAISYEELVGP
Sbjct: 61   EIFGSFHALRTSSIPILDLPAVDESEVFFDARSSAFDYGEVFGGFDGLNFAISYEELVGP 120

Query: 121  SKGVDDGSSDEAWTPAGTESLSDCSDHSGNSHCMSNGDSNQSFGGSTEFCISYNKVNREG 180
             +GVDDGSSDEAWTPAGT+SLSDCSDHSGNSHCMSN DSNQSFG STEF ISYNKVNREG
Sbjct: 121  PQGVDDGSSDEAWTPAGTDSLSDCSDHSGNSHCMSNEDSNQSFGESTEFSISYNKVNREG 180

Query: 181  NGNMSNGKTHVTQLETLPGFSYLVDEAIPSPKGTDDDPSLQTNDDSYLNIDFDTGKVKGK 240
            NGN+S+G+ HVTQLE LPGFSYLVDEA PSPKGT DD SL+TNDDSYLNIDFDTGKVKGK
Sbjct: 181  NGNVSDGEIHVTQLEMLPGFSYLVDEAHPSPKGTYDDLSLKTNDDSYLNIDFDTGKVKGK 240

Query: 241  HPRNTMPPPVDSNGSGQLFEDNHICQNGYGRGGCRSHEDFITVSEISLRTEPSQVPPPSR 300
            HP+NTMPPPVDSNGSG +FED+ ICQN +GR    SHEDFITVSEISLRTEPSQVPPP+R
Sbjct: 241  HPKNTMPPPVDSNGSGNVFEDSLICQNEFGREVFHSHEDFITVSEISLRTEPSQVPPPTR 300

Query: 301  PPPKFAAKKGDYTKMTLNRGEAASEIISDDHSLPLFDVEVDASSSAAASAAAMKEAMVKA 360
            PPPK+AAK GDYTKM LN G+ ASEIISDDH  PLFDVEVDASSSAAASAAAMKEAM KA
Sbjct: 301  PPPKYAAKIGDYTKMALNCGD-ASEIISDDHFNPLFDVEVDASSSAAASAAAMKEAMEKA 360

Query: 361  QAQLQNAKDLWERKKEGVHGRMRLDAKNDMREKEGNLTKIPNRCKSLPNEKVQGIGEIHD 420
            QA+LQ+AKDLWERKKEGVHGRMRLDAK DMREKEGNLTKIPNR KSL +E+VQ IGEIHD
Sbjct: 361  QARLQSAKDLWERKKEGVHGRMRLDAKYDMREKEGNLTKIPNRFKSLTDERVQDIGEIHD 420

Query: 421  YEMNVPTKEERQKALKATEVCSTLYGGEELLTVAEETVPVRSGSRFLVSENYDCCSEWKD 480
            ++MN+P  E+R+K +KATEVCST +GGEELLTV E+TVP+R+GSRFLVS+N+DCCSEWKD
Sbjct: 421  HKMNLPAGEKRKKDVKATEVCSTPFGGEELLTVDEKTVPIRNGSRFLVSDNHDCCSEWKD 480

Query: 481  ATEFFELARADVSEQEFESVNNDEISNSVIGRMGAETTIEAWENQKDQDKQGKPFHTAHV 540
            ATEFFELAR D+S +EFE ++NDEISN V   + AET IEAWEN KDQDK GK FHTAH 
Sbjct: 481  ATEFFELARTDISGKEFELLDNDEISNFVTEPIVAETIIEAWENDKDQDKDGKQFHTAHA 540

Query: 541  LNEEMKNLENLVHGEEEDKAKLKPNRNGTRQKEQVKLKIPQDVY-LEANNKKFEVKIPQG 600
            LNE +KNLEN+VHG+ EDK KLKPN++ TRQKEQVKL   Q V  LEAN++ F V   Q 
Sbjct: 541  LNENVKNLENMVHGKVEDKIKLKPNKDETRQKEQVKLH--QGVCDLEANDRIFGV--AQD 600

Query: 601  FVEVKKQMSGTSELEKHEKPNEFTQLESELKVEQPVSPQDIEQEKKKVVKRKENGNILKE 660
            F+E KK+MS  SELEKHE   EF QL+SELKV+QPVSP DIEQEKKKVVK KENGNILKE
Sbjct: 601  FMEEKKRMSNASELEKHENRIEFKQLDSELKVDQPVSPIDIEQEKKKVVKGKENGNILKE 660

Query: 661  SHRKENNANNLEEAIENEKREKLLAEASEREKLEQKTRMFLEQEENMKRLNLVLEEENFE 720
            SHRKENNAN +EEA+ENEKREKL +EASEREKLEQK  MFLEQEEN KRLNLVL +ENF 
Sbjct: 661  SHRKENNANKMEEALENEKREKLFSEASEREKLEQKKMMFLEQEENKKRLNLVLADENFR 720

Query: 721  GQMSCERQI------EDHGKKEKEAAKVGVSERSKAVHETEDDDKWSKKFQYREECEKGV 780
            GQM+ ER++      EDH K+ KEAAKV VSERSK  HE EDDDKWSK  QYREECE GV
Sbjct: 721  GQMARERELDGVHGKEDHRKRGKEAAKV-VSERSKLAHEREDDDKWSKNIQYREECEIGV 780

Query: 781  EDSFQHLNVEEMPRDAVNCKVTQMLAEDSQESADLEGTSMEHDEIERLKDRHQVNSTIGS 840
            EDSFQ LN+E  P D   CKVTQML EDSQ SADL+G SMEHDE+E+L DRH+VNSTIGS
Sbjct: 781  EDSFQRLNIERRPSDVGKCKVTQMLVEDSQYSADLKGASMEHDELEKLSDRHEVNSTIGS 840

Query: 841  QAYVELEDFGISPAAFQMNDNKNCLPTRLACSTGMPEEFSVVDESEERKIRVTVNDSLPE 900
            QAYVE++D GIS AAFQM+D++N LPT LACS G+PEEFSVVDES ER   VT N +  E
Sbjct: 841  QAYVEVKDLGISAAAFQMHDDQNHLPTVLACSMGVPEEFSVVDESGER-TTVTGNKTSSE 900

Query: 901  SGGIENLQFYKNSCASSMCHAEVEHHKVPVEMEDADIQFSFDELTTKAGKETSFQSKPGH 960
            S GI+NLQF KNS A     AE+EHHKV VEMEDADIQ SF+E T K GKET FQ KPG 
Sbjct: 901  SDGIDNLQFSKNSSALGARQAEIEHHKVSVEMEDADIQLSFNEWTKKVGKETCFQPKPGD 960

Query: 961  TPAEATNSEDAVSFENSTSMDERESEIEENVLKMEDMKTSLPLDITDEKAGQANAGMQEF 1020
            T AEATNSEDA S ENST +DERE EIEEN LKM+DMKTS PLD + E+AGQ+N GM+EF
Sbjct: 961  TQAEATNSEDAFSSENSTCVDEREHEIEENALKMDDMKTSPPLDRSGERAGQSNVGMEEF 1020

Query: 1021 VGRKKFVTRMDSGPEPPERKLFCSMEDKVKSFDQVEDKEHKVSVQGANVRAEKGSGLEST 1080
            VGRK FVTRMD  PE PE  LFC MEDKVK  ++ ED+   VSVQG N++AEKGSG+EST
Sbjct: 1021 VGRKTFVTRMDCDPEQPESNLFCGMEDKVKFSNEAEDEGQTVSVQGVNIKAEKGSGMEST 1080

Query: 1081 RPTISDRTQKSGELAHEVHVNQATERKEKIVN---TSKGKESERVRSEADFENDILRKLE 1140
            RPTIS RTQKSGE A+EV+ NQATERKEK+VN    SKGKESERVRSE++FEND LRKLE
Sbjct: 1081 RPTISGRTQKSGEFAYEVNANQATERKEKLVNQSHKSKGKESERVRSESEFENDRLRKLE 1140

Query: 1141 EEREREREREKDRMPIDKIALEPRDRVGAEARERAERAALERMTAEARQRALAEARERLE 1200
            EEREREREREKDRM ID+IALEPRDRVGAE RERAER ALERMTAEARQRAL EARERLE
Sbjct: 1141 EEREREREREKDRMHIDRIALEPRDRVGAETRERAERTALERMTAEARQRALTEARERLE 1200

Query: 1201 KACAEARENSLAGKATMEARVKAERAAVERATAEARERAAEKAMSDRTSFGVRERMERSV 1260
            KACAEARENSLAGKATMEARVKAERAAVERATAEARERAAEKAMS+RTSFGVRERMERSV
Sbjct: 1201 KACAEARENSLAGKATMEARVKAERAAVERATAEARERAAEKAMSERTSFGVRERMERSV 1260

Query: 1261 SDKFSASSRNNEVRQKFSSSGQPSLQSQSFGSSIASRYAYYSAYDERNEGVDGESPQRCK 1320
            SDKFSASSRNNE+RQK SSSGQPSLQSQSFGSS+ASRYAY+SAYDERNEGVDGESPQRCK
Sbjct: 1261 SDKFSASSRNNEMRQKSSSSGQPSLQSQSFGSSLASRYAYHSAYDERNEGVDGESPQRCK 1320

Query: 1321 ARLERHQRTAERAAKALAEKNMRDLLAQREQAERNRLAETLDADVRRWSSGKEGNLRALL 1380
            ARLERHQRTAERAAKALAEKNMRDLLAQREQAERNRLAETLDADVRRWSSGKEGNLRALL
Sbjct: 1321 ARLERHQRTAERAAKALAEKNMRDLLAQREQAERNRLAETLDADVRRWSSGKEGNLRALL 1380

Query: 1381 STLQYILGPDSGWQPIPLTEVITAAAVKKAYRKATLCVHPDKLQQRGASIQQKYICEKVF 1440
            STLQYILGP SGWQPIPLTEVITAAAVKKAYRKATLCVHPDKLQQRGASIQQKYICEKVF
Sbjct: 1381 STLQYILGPGSGWQPIPLTEVITAAAVKKAYRKATLCVHPDKLQQRGASIQQKYICEKVF 1440

Query: 1441 DLLKEAWNKFNSEER 1446
            DLLKEAWNKFNSEER
Sbjct: 1441 DLLKEAWNKFNSEER 1448

BLAST of Spg013516 vs. NCBI nr
Match: KAG7026783.1 (Auxilin-like protein 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 2174.4 bits (5633), Expect = 0.0e+00
Identity = 1190/1455 (81.79%), Postives = 1282/1455 (88.11%), Query Frame = 0

Query: 1    MDNLSHSRLPNRGSTSVSKKICNGSNGGGPFVAQTIYDDVYGGPPKFGVSALSPRFEDYG 60
            MDNLSHSRLPNRGSTSVSKKICNGSNGGGPFVAQTIYDDVYGGPPKFGVSALSPRFEDYG
Sbjct: 1    MDNLSHSRLPNRGSTSVSKKICNGSNGGGPFVAQTIYDDVYGGPPKFGVSALSPRFEDYG 60

Query: 61   EIFGSFHALRASSIPILDLPAVDESEVFFDARSSAFDYTEVFGGFDGLDFAISYEELVGP 120
            EIFGSFHALR SSIPILDLPAVDESEVFFDARSSAFDY EVFGGFDGL+FAISYEELVGP
Sbjct: 61   EIFGSFHALRTSSIPILDLPAVDESEVFFDARSSAFDYGEVFGGFDGLNFAISYEELVGP 120

Query: 121  SKGVDDGSSDEAWTPAGTESLSDCSDHSGNSHCMSNGDSNQSFGGSTEFCISYNKVNREG 180
             +GVDDGSSDEAWTPAGT+SLSDCSDHSGNSHCMSN DSNQSFG STEF ISYNKVNREG
Sbjct: 121  PQGVDDGSSDEAWTPAGTDSLSDCSDHSGNSHCMSNEDSNQSFGESTEFSISYNKVNREG 180

Query: 181  NGNMSNGKTHVTQLETLPGFSYLVDEAIPSPKGTDDDPSLQTNDDSYLNIDFDTGKVKGK 240
            NGN+S+G+ HVTQLE LPGFSYLVDEA PSPKGT DD SL+TNDDSYLNIDFDTGKVKGK
Sbjct: 181  NGNVSDGEIHVTQLEMLPGFSYLVDEAHPSPKGTYDDLSLKTNDDSYLNIDFDTGKVKGK 240

Query: 241  HPRNTMPPPVDSNGSGQLFEDNHICQNGYGRGGCRSHEDFITVSEISLRTEPSQVPPPSR 300
            HP+NTMPPPVDSNGSG +FED+ ICQN +GR    SHEDFITVSEISLRTEPSQVPPP+R
Sbjct: 241  HPKNTMPPPVDSNGSGNVFEDSLICQNEFGREVFHSHEDFITVSEISLRTEPSQVPPPTR 300

Query: 301  PPPKFAAKKGDYTKMTLNRGEAASEIISDDHSLPLFDVEVDASSSAAASAAAMKEAMVKA 360
            PPPK+AAK GDYTKM LN G+ ASEIISDDH  PLFDVEVDASSSAAASAAAMKEAM KA
Sbjct: 301  PPPKYAAKIGDYTKMALNCGD-ASEIISDDHFNPLFDVEVDASSSAAASAAAMKEAMEKA 360

Query: 361  QAQLQNAKDLWERKKEGVHGRMRLDAKNDMREKEGNLTKIPNRCKSLPNEKVQGIGEIHD 420
            QA+LQ+AKDLWERKKEGVHGRMRLDAK DMREKEGNLTKIPNR KSL +E+VQ IGEIHD
Sbjct: 361  QARLQSAKDLWERKKEGVHGRMRLDAKYDMREKEGNLTKIPNRFKSLTDERVQDIGEIHD 420

Query: 421  YEMNVPTKEERQKALKATEVCSTLYGGEELLTVAEETVPVRSGSRFLVSENYDCCSEWKD 480
            ++MN+P  E+R+K +KATEVCST +GGEELLTV E+TVP+R+GSRFLVS+N+DCCSEWKD
Sbjct: 421  HKMNLPAGEKRKKDVKATEVCSTPFGGEELLTVDEKTVPIRNGSRFLVSDNHDCCSEWKD 480

Query: 481  ATEFFELARADVSEQEFESVNNDEISNSVIGRMGAETTIEAWENQKDQDKQGKPFHTAHV 540
            ATEFFELAR D+S +EFE ++NDEISN V   + AET IEAWEN KDQDK GK FHTAH 
Sbjct: 481  ATEFFELARTDISGKEFELLDNDEISNFVTEPIVAETIIEAWENDKDQDKDGKQFHTAHA 540

Query: 541  LNEEMKNLENLVHGEEEDKAKLKPNRNGTRQKEQVKLKIPQDVY-LEANNKKFEVKIPQG 600
            LNE +KNLEN+VHG+ E+K KLKPN++ TRQKEQVKL   Q V  LEAN++ F V   Q 
Sbjct: 541  LNENVKNLENMVHGKVENKIKLKPNKDETRQKEQVKLH--QGVCDLEANDRIFGV--AQD 600

Query: 601  FVEVKKQMSGTSELEKHEKPNEFTQLESELKVEQPVSPQDIEQEKKKVVKRKENGNILKE 660
            F+E KK+MS  SELEKHE   EF QL+SELKV+QPVSP DIEQEKKKVVK KENGNILKE
Sbjct: 601  FMEEKKRMSNASELEKHENRIEFKQLDSELKVDQPVSPIDIEQEKKKVVKGKENGNILKE 660

Query: 661  SHRKENNANNLEEAIENEKREKLLAEASEREKLEQKTRMFLEQEENMKRLNLVLEEENFE 720
            SHRKENNAN +EEA+ENEKREKL +EASEREKLEQK  MFLEQEEN KRLNLVL +ENF 
Sbjct: 661  SHRKENNANKMEEALENEKREKLFSEASEREKLEQKKMMFLEQEENKKRLNLVLADENFR 720

Query: 721  GQMSCERQI------EDHGKKEKEAAKVGVSERSKAVHETEDDDKWSKKFQYREECEKGV 780
            GQM+ ER++      EDH K+ KEAAKV VSERSK  HE EDDDKWSK  QYREECE GV
Sbjct: 721  GQMARERELDGVHGKEDHRKRGKEAAKV-VSERSKLAHEREDDDKWSKNIQYREECEIGV 780

Query: 781  EDSFQHLNVEEMPRDAVNCKVTQMLAEDSQESADLEGTSMEHDEIERLKDRHQVNSTIGS 840
            EDSFQ LN+E  P D   CKVTQML EDSQ SADL+G SMEHDE+E+L DRH+VNSTIGS
Sbjct: 781  EDSFQRLNIERRPSDVGKCKVTQMLVEDSQYSADLKGASMEHDELEKLSDRHEVNSTIGS 840

Query: 841  QAYVELEDFGISPAAFQMNDNKNCLPTRLACSTGMPEEFSVVDESEERKIRVTVNDSLPE 900
            QAYVE++D GIS AAFQM+D++N LPT LACS G+PEEFSVVDES ER   VT N +  E
Sbjct: 841  QAYVEVKDLGISAAAFQMHDDQNHLPTVLACSMGVPEEFSVVDESGER-TTVTGNKTSSE 900

Query: 901  SGGIENLQFYKNSCASSMCHAEVEHHKVPVEMEDADIQFSFDELTTKAGKETSFQSKPGH 960
            S GI+NLQF KNS A     AE+EHHKV VEMEDADIQ SF+E T K GKET FQ KPG 
Sbjct: 901  SDGIDNLQFSKNSSALGARQAEIEHHKVSVEMEDADIQLSFNEWTKKVGKETCFQPKPGD 960

Query: 961  TPAEATNSEDAVSFENSTSMDERESEIEENVLKMEDMKTSLPLDITDEKAGQANAGMQEF 1020
            T AEATNSEDA S ENST +DERE EIEEN LKM+DMKTS PLD + E+AGQ+N GM+EF
Sbjct: 961  TQAEATNSEDAFSSENSTCVDEREHEIEENALKMDDMKTSPPLDRSGERAGQSNVGMEEF 1020

Query: 1021 VGRKKFVTRMDSGPEPPERKLFCSMEDKVKSFDQVEDKEHKVSVQGANVRAEKGSGLEST 1080
            VGRK FVTRMD  PE PE  LFC MEDKVK  ++ ED+   VSVQG N++AEKGSG+EST
Sbjct: 1021 VGRKTFVTRMDCDPEQPESNLFCGMEDKVKFSNEAEDEGQTVSVQGVNIKAEKGSGMEST 1080

Query: 1081 RPTISDRTQKSGELAHEVHVNQATERKEKIVN---TSKGKESERVRSEADFENDILRKLE 1140
            RPTIS RTQKSGE A+EV+ NQATERKEK+VN    SKGKESERVRSE++FEND LRKLE
Sbjct: 1081 RPTISGRTQKSGEFAYEVNANQATERKEKLVNQSHKSKGKESERVRSESEFENDRLRKLE 1140

Query: 1141 EEREREREREKDRMPIDKIALEPRDRVGAEARERAERAALERMTAEARQRALAEARERLE 1200
            EEREREREREKDRM ID+IALEPRDRVGAE RERAER ALERMTAEARQRAL EARERLE
Sbjct: 1141 EEREREREREKDRMHIDRIALEPRDRVGAETRERAERTALERMTAEARQRALTEARERLE 1200

Query: 1201 KACAEARENSLAGKATMEARVKAERAAVERATAEARERAAEKAMSDRTSFGVRERMERSV 1260
            KACAEARENSLAGKATMEARVKAERAAVERATAEARERAAEKAMS+RTSFGVRERMERSV
Sbjct: 1201 KACAEARENSLAGKATMEARVKAERAAVERATAEARERAAEKAMSERTSFGVRERMERSV 1260

Query: 1261 SDKFSASSRNNEVRQKFSSSGQPSLQSQSFGSSIASRYAYYSAYDERNEGVDGESPQRCK 1320
            SDKFSASSRNNE+RQK SSSGQPSLQSQSFGSS+ASRYAY+SAYDERNEGVDGESPQRCK
Sbjct: 1261 SDKFSASSRNNEMRQKSSSSGQPSLQSQSFGSSLASRYAYHSAYDERNEGVDGESPQRCK 1320

Query: 1321 ARLERHQRTAERAAKALAEKNMRDLLAQREQAERNRLAETLDADVRRWSSGKEGNLRALL 1380
            ARLERHQRTAERAAKALAEKNMRDLLAQREQAERNRLAETLDADVRRWSSGKEGNLRALL
Sbjct: 1321 ARLERHQRTAERAAKALAEKNMRDLLAQREQAERNRLAETLDADVRRWSSGKEGNLRALL 1380

Query: 1381 STLQYILGPDSGWQPIPLTEVITAAAVKKAYRKATLCVHPDKLQQRGASIQQKYICEKVF 1440
            STLQYILGP SGWQPIPLTEVITAAAVKKAYRKATLCVHPDKLQQRGASIQQKYICEKVF
Sbjct: 1381 STLQYILGPGSGWQPIPLTEVITAAAVKKAYRKATLCVHPDKLQQRGASIQQKYICEKVF 1440

Query: 1441 DLLKEAWNKFNSEER 1446
            DLLKEAWNKFNSEER
Sbjct: 1441 DLLKEAWNKFNSEER 1448

BLAST of Spg013516 vs. NCBI nr
Match: XP_022963025.1 (auxilin-like protein 1 [Cucurbita moschata])

HSP 1 Score: 2170.6 bits (5623), Expect = 0.0e+00
Identity = 1189/1455 (81.72%), Postives = 1280/1455 (87.97%), Query Frame = 0

Query: 1    MDNLSHSRLPNRGSTSVSKKICNGSNGGGPFVAQTIYDDVYGGPPKFGVSALSPRFEDYG 60
            MDNLSHSRLPNRGSTSVSKKICNGSNGGGPFVAQTIYDDVYGGPPKFGVSALSPRFEDYG
Sbjct: 1    MDNLSHSRLPNRGSTSVSKKICNGSNGGGPFVAQTIYDDVYGGPPKFGVSALSPRFEDYG 60

Query: 61   EIFGSFHALRASSIPILDLPAVDESEVFFDARSSAFDYTEVFGGFDGLDFAISYEELVGP 120
            EIFGSFHALR SSIPILDLPAVDESEVFFDARSSAFDY EVFGGFDGL+FAISYEELVGP
Sbjct: 61   EIFGSFHALRTSSIPILDLPAVDESEVFFDARSSAFDYGEVFGGFDGLNFAISYEELVGP 120

Query: 121  SKGVDDGSSDEAWTPAGTESLSDCSDHSGNSHCMSNGDSNQSFGGSTEFCISYNKVNREG 180
            S+GVDDGSSDEAWTPAGT+SLSDCSDHSGNSHCMSN DSNQSFG STEF ISYNKVNREG
Sbjct: 121  SQGVDDGSSDEAWTPAGTDSLSDCSDHSGNSHCMSNEDSNQSFGESTEFSISYNKVNREG 180

Query: 181  NGNMSNGKTHVTQLETLPGFSYLVDEAIPSPKGTDDDPSLQTNDDSYLNIDFDTGKVKGK 240
            NGN+S+ K HVTQLE LPGFSYLVDEA PSPKGT DD SL+TNDDSYLNIDFDTGKVKGK
Sbjct: 181  NGNVSDEKIHVTQLEMLPGFSYLVDEAHPSPKGTYDDLSLKTNDDSYLNIDFDTGKVKGK 240

Query: 241  HPRNTMPPPVDSNGSGQLFEDNHICQNGYGRGGCRSHEDFITVSEISLRTEPSQVPPPSR 300
            HP+NTMPPPVDSNGSG +FED+ ICQN +GR    SHEDFITVSEISLRTEPSQVPPP+R
Sbjct: 241  HPKNTMPPPVDSNGSGNVFEDSLICQNEFGREVFHSHEDFITVSEISLRTEPSQVPPPTR 300

Query: 301  PPPKFAAKKGDYTKMTLNRGEAASEIISDDHSLPLFDVEVDASSSAAASAAAMKEAMVKA 360
            PPPK+AAK GDYTKM LN G+ ASEIISDDH  PLFDVEVDASSSAAASAAAMKEAM KA
Sbjct: 301  PPPKYAAKMGDYTKMALNCGD-ASEIISDDHFNPLFDVEVDASSSAAASAAAMKEAMEKA 360

Query: 361  QAQLQNAKDLWERKKEGVHGRMRLDAKNDMREKEGNLTKIPNRCKSLPNEKVQGIGEIHD 420
            QA+LQ+AKDLWERKKEGVHGRMRLDAK DMREKEGNLTKIPNR KSL +E+VQ IGEIHD
Sbjct: 361  QARLQSAKDLWERKKEGVHGRMRLDAKYDMREKEGNLTKIPNRFKSLTDERVQDIGEIHD 420

Query: 421  YEMNVPTKEERQKALKATEVCSTLYGGEELLTVAEETVPVRSGSRFLVSENYDCCSEWKD 480
            ++MN+P  E+R+K +KA EVCST +GGEELLTV E+TVPVR+GSRFLVS+N+DCCSEWKD
Sbjct: 421  HKMNLPAGEKRKKDVKAAEVCSTPFGGEELLTVDEKTVPVRNGSRFLVSDNHDCCSEWKD 480

Query: 481  ATEFFELARADVSEQEFESVNNDEISNSVIGRMGAETTIEAWENQKDQDKQGKPFHTAHV 540
            ATEFFELARAD+S +EFE ++NDEISN V   + AET IEAWEN KDQDK GKPFHTAH 
Sbjct: 481  ATEFFELARADISGKEFELLDNDEISNFVTEPIVAETIIEAWENDKDQDKDGKPFHTAHA 540

Query: 541  LNEEMKNLENLVHGEEEDKAKLKPNRNGTRQKEQVKLKIPQDVY-LEANNKKFEVKIPQG 600
            LNE +K LEN+VHG+ EDK KLKPN++ TRQKEQVKL   Q V  LEAN++ F V   Q 
Sbjct: 541  LNENVKKLENMVHGKVEDKIKLKPNKDETRQKEQVKLH--QGVCDLEANDRMFGV--AQD 600

Query: 601  FVEVKKQMSGTSELEKHEKPNEFTQLESELKVEQPVSPQDIEQEKKKVVKRKENGNILKE 660
            F+E KK+MS  SELEKHE   EF QL+SELKV+QPVSP DIEQEKKKVVK KENGNILKE
Sbjct: 601  FMEEKKRMSNASELEKHENRFEFKQLDSELKVDQPVSPIDIEQEKKKVVKGKENGNILKE 660

Query: 661  SHRKENNANNLEEAIENEKREKLLAEASEREKLEQKTRMFLEQEENMKRLNLVLEEENFE 720
            SHRKENNAN +EEA+ENEKRE+L +EAS REKLE+K  MFLEQEEN KRLNLVL +ENF 
Sbjct: 661  SHRKENNANKMEEALENEKREELFSEASVREKLEKKKMMFLEQEENKKRLNLVLADENFR 720

Query: 721  GQMSCERQI------EDHGKKEKEAAKVGVSERSKAVHETEDDDKWSKKFQYREECEKGV 780
            GQM+ ER++      EDHGK+ KEAAKV VSERSK  HE EDDDKWSK  QYREECE GV
Sbjct: 721  GQMARERELDGVHGKEDHGKRVKEAAKV-VSERSKLAHEREDDDKWSKNIQYREECEIGV 780

Query: 781  EDSFQHLNVEEMPRDAVNCKVTQMLAEDSQESADLEGTSMEHDEIERLKDRHQVNSTIGS 840
            EDSFQ LN+E  P D   CKV QML EDSQ SADL+G SMEHDE+E+L DRH+VNSTIGS
Sbjct: 781  EDSFQRLNIERRPGDVGKCKVPQMLVEDSQYSADLKGASMEHDELEKLSDRHEVNSTIGS 840

Query: 841  QAYVELEDFGISPAAFQMNDNKNCLPTRLACSTGMPEEFSVVDESEERKIRVTVNDSLPE 900
            QAYVE++D GIS AAFQM+D++N LPT LACS G+PEEFSVVDES ER   VT N +  E
Sbjct: 841  QAYVEVKDLGISAAAFQMHDDQNHLPTVLACSMGVPEEFSVVDESGER-TTVTGNKTSSE 900

Query: 901  SGGIENLQFYKNSCASSMCHAEVEHHKVPVEMEDADIQFSFDELTTKAGKETSFQSKPGH 960
            S GI+NLQF KNS A     AE+EHHKV VEMEDADIQ SF+E T K GKET FQ KPG 
Sbjct: 901  SDGIDNLQFSKNSSALGARQAEIEHHKVSVEMEDADIQLSFNEWTKKVGKETCFQPKPGD 960

Query: 961  TPAEATNSEDAVSFENSTSMDERESEIEENVLKMEDMKTSLPLDITDEKAGQANAGMQEF 1020
            T AEATNSEDA S ENST +DERE EIEEN LKM+DMKTS PLD + E+A Q+N GM+EF
Sbjct: 961  TQAEATNSEDAFSSENSTCVDEREHEIEENALKMDDMKTSPPLDRSGERADQSNVGMEEF 1020

Query: 1021 VGRKKFVTRMDSGPEPPERKLFCSMEDKVKSFDQVEDKEHKVSVQGANVRAEKGSGLEST 1080
            VGRK FVTRMD  PE PE  LFC MEDKVK  ++ ED+   VSVQG N++AEKGSG+EST
Sbjct: 1021 VGRKTFVTRMDCDPEQPESNLFCRMEDKVKFSNEAEDEGQTVSVQGVNIKAEKGSGMEST 1080

Query: 1081 RPTISDRTQKSGELAHEVHVNQATERKEKIVN---TSKGKESERVRSEADFENDILRKLE 1140
            RPTIS RTQKSGE A+EV+ NQATERKEK+VN    SKGKESERVRSE++FEND LRKLE
Sbjct: 1081 RPTISGRTQKSGEFAYEVNANQATERKEKLVNQSHKSKGKESERVRSESEFENDRLRKLE 1140

Query: 1141 EEREREREREKDRMPIDKIALEPRDRVGAEARERAERAALERMTAEARQRALAEARERLE 1200
            EEREREREREKDRM ID+IALEPRDRVGAE RERAER ALERMTAEARQRAL EARERLE
Sbjct: 1141 EEREREREREKDRMHIDRIALEPRDRVGAETRERAERTALERMTAEARQRALTEARERLE 1200

Query: 1201 KACAEARENSLAGKATMEARVKAERAAVERATAEARERAAEKAMSDRTSFGVRERMERSV 1260
            KACAEARENSLAGKATMEARVKAERAAVERATAEARERAAEKAMS+RTSFGVRERMERSV
Sbjct: 1201 KACAEARENSLAGKATMEARVKAERAAVERATAEARERAAEKAMSERTSFGVRERMERSV 1260

Query: 1261 SDKFSASSRNNEVRQKFSSSGQPSLQSQSFGSSIASRYAYYSAYDERNEGVDGESPQRCK 1320
            SDKFSASSRNNE+RQK SSSGQPSLQSQSFGSS+ASRYAY+SAYDERNEGVDGESPQRCK
Sbjct: 1261 SDKFSASSRNNEMRQKSSSSGQPSLQSQSFGSSLASRYAYHSAYDERNEGVDGESPQRCK 1320

Query: 1321 ARLERHQRTAERAAKALAEKNMRDLLAQREQAERNRLAETLDADVRRWSSGKEGNLRALL 1380
            ARLERHQRTAERAAKALAEKNMRDLLAQREQAERNRLAETLDADVRRWSSGKEGNLRALL
Sbjct: 1321 ARLERHQRTAERAAKALAEKNMRDLLAQREQAERNRLAETLDADVRRWSSGKEGNLRALL 1380

Query: 1381 STLQYILGPDSGWQPIPLTEVITAAAVKKAYRKATLCVHPDKLQQRGASIQQKYICEKVF 1440
            STLQYILGP SGWQPIPLTEVITAAAVKKAYRKATLCVHPDKLQQRGASIQQKYICEKVF
Sbjct: 1381 STLQYILGPGSGWQPIPLTEVITAAAVKKAYRKATLCVHPDKLQQRGASIQQKYICEKVF 1440

Query: 1441 DLLKEAWNKFNSEER 1446
            DLLKEAWNKFNSEER
Sbjct: 1441 DLLKEAWNKFNSEER 1448

BLAST of Spg013516 vs. NCBI nr
Match: XP_023003995.1 (uncharacterized protein LOC111497445 [Cucurbita maxima])

HSP 1 Score: 2165.6 bits (5610), Expect = 0.0e+00
Identity = 1186/1455 (81.51%), Postives = 1278/1455 (87.84%), Query Frame = 0

Query: 1    MDNLSHSRLPNRGSTSVSKKICNGSNGGGPFVAQTIYDDVYGGPPKFGVSALSPRFEDYG 60
            MDNLSHSRLPNRGSTSVSKKICNGSNGGGPF+AQTIYDDVYGGPPKFGVSALSPRFEDYG
Sbjct: 1    MDNLSHSRLPNRGSTSVSKKICNGSNGGGPFIAQTIYDDVYGGPPKFGVSALSPRFEDYG 60

Query: 61   EIFGSFHALRASSIPILDLPAVDESEVFFDARSSAFDYTEVFGGFDGLDFAISYEELVGP 120
            EIFGSFHALR SSIPILDLPAVDESEVFFDARSSAFDY EVFGGFDGL+FAISYEELVGP
Sbjct: 61   EIFGSFHALRTSSIPILDLPAVDESEVFFDARSSAFDYAEVFGGFDGLNFAISYEELVGP 120

Query: 121  SKGVDDGSSDEAWTPAGTESLSDCSDHSGNSHCMSNGDSNQSFGGSTEFCISYNKVNREG 180
            S+GVDDGSSDEAWTPAGT+SLSDCSDHSGNSHCMSN DSNQSFG STEF ISYNKVNREG
Sbjct: 121  SQGVDDGSSDEAWTPAGTDSLSDCSDHSGNSHCMSNEDSNQSFGESTEFSISYNKVNREG 180

Query: 181  NGNMSNGKTHVTQLETLPGFSYLVDEAIPSPKGTDDDPSLQTNDDSYLNIDFDTGKVKGK 240
            NGN+S+GK HVTQLE LPGFSYLVDEA PSPKGT DD SL+TNDDSYLNIDFDTGKVKGK
Sbjct: 181  NGNVSDGKIHVTQLEMLPGFSYLVDEAHPSPKGTYDDLSLKTNDDSYLNIDFDTGKVKGK 240

Query: 241  HPRNTMPPPVDSNGSGQLFEDNHICQNGYGRGGCRSHEDFITVSEISLRTEPSQVPPPSR 300
            HP+NTMPPPVDSNGSG +FED+ ICQN +GR    SHEDFITVSEISLRTEPSQVPPP+R
Sbjct: 241  HPKNTMPPPVDSNGSGHVFEDSLICQNEFGREVFHSHEDFITVSEISLRTEPSQVPPPTR 300

Query: 301  PPPKFAAKKGDYTKMTLNRGEAASEIISDDHSLPLFDVEVDASSSAAASAAAMKEAMVKA 360
            PPPK+AAK GDYTKM LN  E ASEIISDDH  PLF+VEVDA SSAAASAAAMKEAM KA
Sbjct: 301  PPPKYAAKMGDYTKMALN-CEDASEIISDDHFNPLFNVEVDAGSSAAASAAAMKEAMEKA 360

Query: 361  QAQLQNAKDLWERKKEGVHGRMRLDAKNDMREKEGNLTKIPNRCKSLPNEKVQGIGEIHD 420
            QA+LQ+AKDLWERKKEGVHGRMRLDAK DMREKEGNLTKIPNR K   +E+VQGIGEIHD
Sbjct: 361  QARLQSAKDLWERKKEGVHGRMRLDAKYDMREKEGNLTKIPNRFKGFADERVQGIGEIHD 420

Query: 421  YEMNVPTKEERQKALKATEVCSTLYGGEELLTVAEETVPVRSGSRFLVSENYDCCSEWKD 480
            ++MN P  E+RQK +KATEV STL+GGEELLTVAE+TV +R+GSRFLVS+N+DCCSEWKD
Sbjct: 421  HKMNFPAGEKRQKDVKATEVSSTLFGGEELLTVAEKTVSIRNGSRFLVSDNHDCCSEWKD 480

Query: 481  ATEFFELARADVSEQEFESVNNDEISNSVIGRMGAETTIEAWENQKDQDKQGKPFHTAHV 540
            ATEFFELARAD+S +EFE ++NDEISN V G + AET I+AWEN KDQDK GKPFHTAH 
Sbjct: 481  ATEFFELARADISGKEFELLDNDEISNFVTGPIVAETIIQAWENDKDQDKDGKPFHTAHA 540

Query: 541  LNEEMKNLENLVHGEEEDKAKLKPNRNGTRQKEQVKLKIPQDVY-LEANNKKFEVKIPQG 600
            LNE +KNLEN+VHG+ EDK KLKPN++ TRQKEQVKL   Q V  LEAN++ F V   Q 
Sbjct: 541  LNENVKNLENMVHGKVEDKIKLKPNKDETRQKEQVKLH--QGVCDLEANDRIFGV--AQD 600

Query: 601  FVEVKKQMSGTSELEKHEKPNEFTQLESELKVEQPVSPQDIEQEKKKVVKRKENGNILKE 660
            F+E KK+ S  SELEKHE   EF QL+SELKV+QPVSP DIEQEKKKVVK KENGNILKE
Sbjct: 601  FMEEKKRTSNASELEKHENRIEFKQLDSELKVDQPVSPIDIEQEKKKVVKGKENGNILKE 660

Query: 661  SHRKENNANNLEEAIENEKREKLLAEASEREKLEQKTRMFLEQEENMKRLNLVLEEENFE 720
            SHRKENNAN +EEA+ENEKREKL +EASEREKLEQK  MFLEQEEN KRLNLVL +ENF 
Sbjct: 661  SHRKENNANKMEEALENEKREKLFSEASEREKLEQKKMMFLEQEENKKRLNLVLADENFR 720

Query: 721  GQMSCERQI------EDHGKKEKEAAKVGVSERSKAVHETEDDDKWSKKFQYREECEKGV 780
             Q++ E ++      EDHGK+ KEAAKV VSERSK  HE EDDDKWSK  QYREECE GV
Sbjct: 721  RQVAREIELDGVHGKEDHGKRGKEAAKV-VSERSKLAHEREDDDKWSKNIQYREECEIGV 780

Query: 781  EDSFQHLNVEEMPRDAVNCKVTQMLAEDSQESADLEGTSMEHDEIERLKDRHQVNSTIGS 840
            +DSFQ LN+E  P D   CKVTQML EDSQ SADL+G SMEHDE+E+L DRH+VNSTIGS
Sbjct: 781  KDSFQRLNIERRPSDVGKCKVTQMLVEDSQYSADLKGASMEHDELEKLSDRHEVNSTIGS 840

Query: 841  QAYVELEDFGISPAAFQMNDNKNCLPTRLACSTGMPEEFSVVDESEERKIRVTVNDSLPE 900
            QAYVE++D GIS AAFQM+D++N LPT LACS G+PEEFSVVDES  R   VT N +  E
Sbjct: 841  QAYVEVKDLGISAAAFQMHDDQNHLPTVLACSIGVPEEFSVVDESGVR-TTVTGNKTSSE 900

Query: 901  SGGIENLQFYKNSCASSMCHAEVEHHKVPVEMEDADIQFSFDELTTKAGKETSFQSKPGH 960
            S GI+NLQF KNS A     AE+EHHKV VEMEDADIQ SF+E T K GKETSFQ KPG 
Sbjct: 901  SDGIDNLQFSKNSSALGARQAEIEHHKVSVEMEDADIQLSFNEWTKKVGKETSFQPKPGD 960

Query: 961  TPAEATNSEDAVSFENSTSMDERESEIEENVLKMEDMKTSLPLDITDEKAGQANAGMQEF 1020
            T AEATNSEDA S ENST +DERE++IEEN LKM+DMKTS PLD + E+AGQ+N GM+EF
Sbjct: 961  TQAEATNSEDAFSSENSTCVDERENKIEENALKMDDMKTSPPLDRSGERAGQSNVGMEEF 1020

Query: 1021 VGRKKFVTRMDSGPEPPERKLFCSMEDKVKSFDQVEDKEHKVSVQGANVRAEKGSGLEST 1080
            VGRK FVTR D  PE PE  LFC MEDKVK  +Q ED+   VSVQG N++AEKGSG+EST
Sbjct: 1021 VGRKTFVTRRDCDPEQPESNLFCRMEDKVKFSNQAEDEGQTVSVQGVNIKAEKGSGMEST 1080

Query: 1081 RPTISDRTQKSGELAHEVHVNQATERKEKIVN---TSKGKESERVRSEADFENDILRKLE 1140
            RPTIS RTQKSGE A+EV+ NQATERKEK+VN    SKGKESERVRSEA+FEND+LRKLE
Sbjct: 1081 RPTISGRTQKSGEFAYEVNANQATERKEKLVNQSHKSKGKESERVRSEAEFENDMLRKLE 1140

Query: 1141 EEREREREREKDRMPIDKIALEPRDRVGAEARERAERAALERMTAEARQRALAEARERLE 1200
            EEREREREREKDRM ID+IALEPRDRVGAE RERAER ALERMTAEARQRAL EARERLE
Sbjct: 1141 EEREREREREKDRMHIDRIALEPRDRVGAETRERAERTALERMTAEARQRALTEARERLE 1200

Query: 1201 KACAEARENSLAGKATMEARVKAERAAVERATAEARERAAEKAMSDRTSFGVRERMERSV 1260
            KACAEARENSLAGKATMEARVKAERAAVERATAEARERAAEKAMSDRTSFGVRERMERSV
Sbjct: 1201 KACAEARENSLAGKATMEARVKAERAAVERATAEARERAAEKAMSDRTSFGVRERMERSV 1260

Query: 1261 SDKFSASSRNNEVRQKFSSSGQPSLQSQSFGSSIASRYAYYSAYDERNEGVDGESPQRCK 1320
            SDKFSASSRNNE+RQK SSSGQPSLQSQSFGSS+ SRYAY+SAYDERN GVDGESPQRCK
Sbjct: 1261 SDKFSASSRNNEMRQKSSSSGQPSLQSQSFGSSLDSRYAYHSAYDERNGGVDGESPQRCK 1320

Query: 1321 ARLERHQRTAERAAKALAEKNMRDLLAQREQAERNRLAETLDADVRRWSSGKEGNLRALL 1380
            ARLERHQRTA RAAKALAEKNMRDLLAQREQAERNRLAETLDADVRRWSSGKEGNLRALL
Sbjct: 1321 ARLERHQRTAGRAAKALAEKNMRDLLAQREQAERNRLAETLDADVRRWSSGKEGNLRALL 1380

Query: 1381 STLQYILGPDSGWQPIPLTEVITAAAVKKAYRKATLCVHPDKLQQRGASIQQKYICEKVF 1440
            STLQYILGP SGWQPIPLTEVITAAAVKKAYRKATLCVHPDKLQQRGASIQQKYICEKVF
Sbjct: 1381 STLQYILGPGSGWQPIPLTEVITAAAVKKAYRKATLCVHPDKLQQRGASIQQKYICEKVF 1440

Query: 1441 DLLKEAWNKFNSEER 1446
            DLLKEAWNKFNSEER
Sbjct: 1441 DLLKEAWNKFNSEER 1448

BLAST of Spg013516 vs. NCBI nr
Match: XP_022132574.1 (auxilin-like protein 1 [Momordica charantia])

HSP 1 Score: 2159.4 bits (5594), Expect = 0.0e+00
Identity = 1168/1456 (80.22%), Postives = 1282/1456 (88.05%), Query Frame = 0

Query: 1    MDNLSHSRLPNRGSTSVSKKICNGSNGGGPFVAQTIYDDVYGGPPKFGVSALSPRFEDYG 60
            MDNLSHSRLPNRGSTSVSK ICNGSNGGG FV  TIYDDVYGGPPKFGVSALSPRFEDYG
Sbjct: 1    MDNLSHSRLPNRGSTSVSKNICNGSNGGGAFVPHTIYDDVYGGPPKFGVSALSPRFEDYG 60

Query: 61   EIFGSFHALRASSIPILDLPAVDESEVFFDARSSAFDYTEVFGGFDGLDFAISYEELVGP 120
            EIFGSFHALRASSIPILDLPAVDE+EVFFDARSSAFDY +VFGGFDGLDFAISYEEL+ P
Sbjct: 61   EIFGSFHALRASSIPILDLPAVDETEVFFDARSSAFDYADVFGGFDGLDFAISYEELIAP 120

Query: 121  SKGVDDGSSDEAWTPAGTESLSDCSDHSGNSHCMSNGDSNQSFGGSTEFCISYNKVNREG 180
            SKGVDDGSSDEAWTPAGTESLSDCSDHSGNS C+SNGDSNQSF  STEFCISYNKVNREG
Sbjct: 121  SKGVDDGSSDEAWTPAGTESLSDCSDHSGNSQCISNGDSNQSFSESTEFCISYNKVNREG 180

Query: 181  NGNMSNGKTHVTQLETLPGFSYLVDEAIPSPKGTDDDPSLQTNDDSYLNIDFDTGKVKGK 240
            NGN+SNGKTHVTQLE LPGFSYLVDEA PS  GT DDPSLQTNDDSYLNIDF TGKV+GK
Sbjct: 181  NGNISNGKTHVTQLEMLPGFSYLVDEAQPSAMGTVDDPSLQTNDDSYLNIDFSTGKVRGK 240

Query: 241  HPRNTMPPPVDSNGSGQLFEDNHICQNGYGRGGCRSHEDFITVSEISLRTEPSQVPPPSR 300
            HPRN+MPPP+DSNG GQ F+DN I  NG+GRG CR +EDFITVSEISLRTEPSQVPPPSR
Sbjct: 241  HPRNSMPPPLDSNGCGQSFKDNLISHNGHGRGVCRPYEDFITVSEISLRTEPSQVPPPSR 300

Query: 301  PPPKFAAKKGDYTKMTLNRGEAASEIISDDHSLPLFDVEVDASSSAAASAAAMKEAMVKA 360
            PPP FAA+ GD TKMTLN  EAASE+I D  SLPLFDVEVD+SSSAAASAAAMKEAM KA
Sbjct: 301  PPPMFAAQNGDCTKMTLNCREAASEVILDGPSLPLFDVEVDSSSSAAASAAAMKEAMEKA 360

Query: 361  QAQLQNAKDLWERKKEGVHGRMRLDAKNDMREKEGNLTKIPNRCKSLPNEKVQGIGEIHD 420
            QAQLQNAKDLWERKKEGVHGRMRLDAKNDMR+KEG+LTKIPNRCK+L NEKVQGIGEIHD
Sbjct: 361  QAQLQNAKDLWERKKEGVHGRMRLDAKNDMRDKEGHLTKIPNRCKNLTNEKVQGIGEIHD 420

Query: 421  YEMNVPTKEERQKALKATEVCSTLYGGEELLTVAEETVPVRSGSRFLVSENYDCCSEWKD 480
            +EM VP +EERQK +KATEVCST YGG+E LTVAE+ +P RSGSRFL S+NY CCSEWK+
Sbjct: 421  HEMKVPAREERQKDVKATEVCSTPYGGDEFLTVAEKYLPNRSGSRFLASDNYGCCSEWKE 480

Query: 481  ATEFFELARADVSEQEFESVNNDEISNSVIGRMGAETTIEAWENQKDQDKQGKPFHTAHV 540
            ATEFF LA AD+ E+EFESVNNDEISN VIG+MGAET+ EAWEN+K++DK GKPFH AH+
Sbjct: 481  ATEFFVLAGADMPEKEFESVNNDEISNFVIGQMGAETSTEAWENEKNKDKLGKPFHKAHL 540

Query: 541  LNEEMKNLENLVHGEEEDKAKLKPNRNGTRQKEQVKLKIPQDVY-LEANNKKFEVKIPQG 600
            +N+E+KNLEN+V G+E+DK K KP++N TRQ+E VKLKIPQ V+ LEAN+KKF V   Q 
Sbjct: 541  VNDEVKNLENIVLGKEKDKIKPKPSKNETRQREVVKLKIPQGVFDLEANDKKFGV--AQE 600

Query: 601  FVEVKKQMSGTSELEKHEKPNEFTQLESELKVEQPVSPQDIEQEKKKVVKRKENGNILKE 660
            FVEVK+ MSG S+LEK EKP EF QLESEL+VEQPVSP+DI+++KK  VK KE  NI KE
Sbjct: 601  FVEVKRPMSGASKLEKLEKPIEFKQLESELRVEQPVSPKDIDEKKKNAVKGKEKENIFKE 660

Query: 661  SHRKENNANNLEEAIENEKREKLLAEASEREKLEQKTRMFLEQEENMKRLNLVL-EEENF 720
            SHRKENN N +EE I NEK+EKL  E SEREKLEQK RM  EQ+EN +RLN+ L EEE+ 
Sbjct: 661  SHRKENNENKMEETIGNEKKEKLRTETSEREKLEQKMRMVFEQQENKERLNMALEEEEDL 720

Query: 721  EGQMSCERQI------EDHGKKEKEAAKVGVSERSKAVHETEDDDKWSKKFQYREECEKG 780
            + +M+ ERQ+      EDH KKE EA KVG+SER K  HETE D KW K FQYREECEKG
Sbjct: 721  KERMAGERQLEGVHEMEDHEKKENEAVKVGLSERFKLAHETEVDVKWLKDFQYREECEKG 780

Query: 781  VEDSFQHLNVEEMPRDAVNCKVTQMLAEDSQESADLEGTSMEHDEIERLKDRHQVNSTIG 840
            VED FQ +N+E MPRD   CK TQML +DSQE+A+L+GTSM HDEIERL DR +VN TIG
Sbjct: 781  VEDLFQRVNIEGMPRDVGKCKETQMLVKDSQENAELKGTSMVHDEIERL-DRQKVNPTIG 840

Query: 841  SQAYVELEDFGISPAAFQMNDNKNCLPTRLACSTGMPEEFSVVDESEERKIRVTVNDSLP 900
            +QAY+E+ED GIS AAFQM+D++N LPT L  STG+  EF+VVDES ER+ +V +N+S  
Sbjct: 841  TQAYMEVEDLGISTAAFQMDDDENHLPTILDFSTGVSLEFAVVDESGEREAKVIINESSS 900

Query: 901  ESGGIENLQFYKNSCASSMCHAEVEHHKVPVEMEDADIQFSFDELTTKAGKETSFQSKPG 960
            ESGGIENLQF K+SCASS+  AEVEHHKVPV+MEDA+I    DE T K+GKETSFQ KP 
Sbjct: 901  ESGGIENLQFNKDSCASSLFQAEVEHHKVPVKMEDANIPLPLDEWTKKSGKETSFQPKPD 960

Query: 961  HTPAEATNSEDAVSFENSTSMDERESEIEENVLKMEDMKTSLPLDITDEKAGQANAGMQE 1020
            +T  E TN EDAVS ENSTSMDE E+EIEEN LKMEDMKTSLPLD +DEKAGQA+AGM+E
Sbjct: 961  YTQEEVTNLEDAVSSENSTSMDEGENEIEENELKMEDMKTSLPLDRSDEKAGQADAGMEE 1020

Query: 1021 FVGRKKFVTRMDSGPEPPERKLFCSMEDKVKSFDQVEDKEHKVSVQGANVRAEKGSGLES 1080
            F+GR KFV+R+DS PE PERKLF  MEDK KSF QVE+KE KV+ Q  NVRA+KGSG+E+
Sbjct: 1021 FIGRMKFVSRVDSDPEHPERKLFGCMEDKTKSFHQVEEKEQKVAAQEVNVRADKGSGMET 1080

Query: 1081 TRPTISDRTQKSGELAHEVHVNQATERKEKIVN---TSKGKESERVRSEADFENDILRKL 1140
             +PTIS+R  KS +++HEV+ NQ TERKEKI+N   TSKGKESERVR EA+FEND+LRKL
Sbjct: 1081 AQPTISERIHKSSKVSHEVNANQPTERKEKIINQSHTSKGKESERVRREAEFENDVLRKL 1140

Query: 1141 EEEREREREREKDRMPIDKIALEPRDRVGAEARERAERAALERMTAEARQRALAEARERL 1200
            EEEREREREREKDRMPID+IALEPRDRVGAEARERAERAALERMTAEARQRALA+ARERL
Sbjct: 1141 EEEREREREREKDRMPIDRIALEPRDRVGAEARERAERAALERMTAEARQRALADARERL 1200

Query: 1201 EKACAEARENSLAGKATMEARVKAERAAVERATAEARERAAEKAMSDRTSFGVRERMERS 1260
            EKACAEARENS AGK TMEARVKAERAAVERATAEARERAAEK +SDRTSFGVRER+ERS
Sbjct: 1201 EKACAEARENSFAGKTTMEARVKAERAAVERATAEARERAAEKVISDRTSFGVRERIERS 1260

Query: 1261 VSDKFSASSRNNEVRQKFSSSGQPSLQSQSFGSSIASRYAYYSAYDERNEGVDGESPQRC 1320
            VSDKFSASSRNNE+RQK SSSGQPSLQSQSFGSSIASRY YYSAYDERNEGVDGESPQRC
Sbjct: 1261 VSDKFSASSRNNEMRQKSSSSGQPSLQSQSFGSSIASRYGYYSAYDERNEGVDGESPQRC 1320

Query: 1321 KARLERHQRTAERAAKALAEKNMRDLLAQREQAERNRLAETLDADVRRWSSGKEGNLRAL 1380
            KAR ERHQRTAERAAKALAEKNMRDL+AQREQAERNRLAETLDADVRRWSSGKEGNLRAL
Sbjct: 1321 KARFERHQRTAERAAKALAEKNMRDLVAQREQAERNRLAETLDADVRRWSSGKEGNLRAL 1380

Query: 1381 LSTLQYILGPDSGWQPIPLTEVITAAAVKKAYRKATLCVHPDKLQQRGASIQQKYICEKV 1440
            LSTLQYILGPDSGWQPIPLTEVITAAAVKKAYRKATLCVHPDKLQQRGASIQQKYICEKV
Sbjct: 1381 LSTLQYILGPDSGWQPIPLTEVITAAAVKKAYRKATLCVHPDKLQQRGASIQQKYICEKV 1440

Query: 1441 FDLLKEAWNKFNSEER 1446
            FDLLKEAWNKFNSEER
Sbjct: 1441 FDLLKEAWNKFNSEER 1453

BLAST of Spg013516 vs. ExPASy Swiss-Prot
Match: Q9FWS1 (Auxilin-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=AUL1 PE=2 SV=2)

HSP 1 Score: 307.8 bits (787), Expect = 6.7e-82
Identity = 440/1532 (28.72%), Postives = 710/1532 (46.34%), Query Frame = 0

Query: 14   STSVSKKICNGSNGGGPFVAQTIYDDVYGGPPKFGVSALSPRFEDYGEIFGSFHALRASS 73
            +T+ S+KI N  +    F A  +YD V+  P    V++ SP   DYGEIF        SS
Sbjct: 9    ATTFSRKISNNRSHSLSFSANAVYDGVFSSP----VNSKSP-LVDYGEIFRG-SGPSPSS 68

Query: 74   IPILDLPAVDESEVFFDARSSAFDYTEVFGGFDGLDFAISYEELVGPSKGVDDGSSDEAW 133
            IP LD+P ++  +V  D RSS  DY+ VFGG    DFA++ +E++  S+     + D+  
Sbjct: 69   IPFLDVPELNVGKVKVDVRSSKLDYSSVFGGLGACDFAVTPKEVIIKSEKKTSINEDKKR 128

Query: 134  TPAGTESLSDCSDHSGNSHCMSNGDSNQSFGGSTEFCISYNKVNREGNGNMSNGKTHVTQ 193
                  + SD           + G  +          ISY++          NG TH+TQ
Sbjct: 129  NRRKGGNSSDVP-------LCNEGKKSPEMVRMKHSDISYHQTVPRN----ENGATHLTQ 188

Query: 194  L-ETLPGFSYLVDEAIPSPKGTDDDPSLQTNDDSYLNIDFDTGKVKGKHPRNTMPPPVD- 253
            +  T+PG         P P    D+ SL    +S          V+ K P N     V  
Sbjct: 189  VPATMPG---------PIPTQVVDNTSLLHKIESK---STPIPAVEKKLPCNEGREEVKA 248

Query: 254  SNGSGQLFEDNHICQNGYGRGGCRSHEDFITVSEISLRTEPSQVPPPSRPPPKFAAKKGD 313
            S   G   E +   +N + R GC + +D  T   +S   E   V PPS        + G 
Sbjct: 249  SRKQGSKTEVDF--ENIFARDGCSTRDD-STCKTVS-NGEYRDVKPPSSFQCTLNGEHGA 308

Query: 314  YTKMT-LNRGEA---ASEIISDDHSLPLFDVEVDASSSAAASAAAMKEAMVKAQAQLQNA 373
              +++ LN G +    +E      S P FD E D +S AA S+AA+K+A+ +AQ ++  A
Sbjct: 309  SERLSGLNSGPSERYETEDADSPSSPPYFDAETDENSVAAESSAALKKAIEEAQIRMNIA 368

Query: 374  KDLWERKKEGVHGRMRLDAKNDMR-EKEGNLTKIPNRCKSLPNEKVQGIGEIHDYEMNVP 433
            K + E+KK G     +L + +D + E +GN TK+    +   +   Q +GE+        
Sbjct: 369  KQMMEKKKSGFRSCAKLKSCDDSKIENKGN-TKVEGITEESRDNNSQILGEMVKPSEQSF 428

Query: 434  TKEERQKALKATEVCSTLYG-GEELLTVAEETVPVRSGSRFLVSENYDCCSEWKDATEFF 493
            + E  Q A +A +    L+G  E LL    +  P     + +++   +   + +   + +
Sbjct: 429  SNEGDQHAKRARK----LWGVPEGLLKSTSDHKPEELEEQDIITLEEE---QARRGRKHW 488

Query: 494  ELARADVSEQEFESVNN---DEISNSVIGRMGAETTIEAWENQKDQDKQGKPFHTAHVLN 553
            EL         F+SV N    E  N    +   +T       Q+ Q     PF+T   L 
Sbjct: 489  ELPGG-----IFKSVMNSKQQEPENLAPAKPEPDT------KQEVQPITENPFYTFGQLG 548

Query: 554  EEMKNLENLVHGEEEDKAKLKPNRNGTRQKEQVKLKIPQDVYLEANNKKFEVKIPQGFVE 613
             ++K +     G    K   K  +  T ++     ++ QD   E+++++    IP     
Sbjct: 549  SKLKCVVEAFTG---SKVSQKDEKQFTEKENSTVTQMVQDE--ESDSQEMLAGIPVIETY 608

Query: 614  VKKQMSGTSELEKHEKPNEFTQLESELKVEQPVSPQDIEQEKKKVVK---RKENGNILKE 673
            +++      + E   + N   + ES +      S Q++E+E    VK   + E+G+ +K+
Sbjct: 609  LREVEETPQQTESKSEMNIEEKSESTICAFTERSSQNMEKETGWQVKSACKFEDGSGVKD 668

Query: 674  SHRKENNANNLEEAIENEKREKLLAEASER--EKLEQKTRMFLEQEENMKRLNLVLEEEN 733
                + N ++    ++ E  +++++E  E      + KT +   +E     LN    +++
Sbjct: 669  F---QENGDHTCNVLDQEGEKEIVSEPQEMLVGPDDSKTYVREVEETPTPSLNKTQSDDS 728

Query: 734  FEGQMSCER-QIEDHGKKEKEAAKVGVSERSKAVHETEDD------------DKWSKKFQ 793
                +S  R  I + G  ++    V    R + V +T +D              W  +  
Sbjct: 729  VGAMVSFNRVNISEPGNIDEVQEAVHKVPRRRRVWKTSEDVYNMIKAPKGNNRPWQLESA 788

Query: 794  YREEC-----EKGVEDSFQHLNVEEMPRDA------VNCKVTQMLAEDSQESADLEGTSM 853
              E       E+GV        +E     A       N  V + +     ++AD +G   
Sbjct: 789  ENETTAMSFHEEGVRIHHASEEIESTSGQASDSGLQENWTVLKQMFRQMFQTADTKGEDE 848

Query: 854  EHDEIERLK---DRHQVNSTIGSQAYVELEDFGISPAAF-QMNDNKNCLPTRLACSTGMP 913
             +  +E  +   D HQ       Q  VE         A  + N++++   T      G  
Sbjct: 849  TYCLVESERGHLDIHQKAQEKYEQVEVETVRTNYEAYAHTRENEDESAQETYCRKEDGKV 908

Query: 914  E---EFSVVDESEERKIRVT------VNDSLPESGGIENLQFYKNSCASSMCHAEVEHH- 973
            E   + S+V E    ++ +       V ++  E+G ++ L         +  HAE+  + 
Sbjct: 909  EVQGKTSLVRELIGEELEMASLEEEDVQEASEEAGWVQGLSELNEINEHADSHAEMLEYD 968

Query: 974  --------------KVPVEMEDADIQFSFDELTTKAGKETSFQSKPGHTPAE-------- 1033
                          +   + E+  I  S D  T++    +SF+ + G +  E        
Sbjct: 969  RSETDSNNSRERFDQTQEQAEETMIDGSIDTDTSR----SSFEMRQGDSYIEEVGIEQHR 1028

Query: 1034 ----------ATNSEDAVSFENSTSMDERESEIEENVLKMEDMKTSL------PLDITDE 1093
                       +N+E+ +   +S S+    S +E++   ++D   S        L+ T E
Sbjct: 1029 SDQFPEKASAVSNTEEHIEEIDSDSIQSGWSVVEDDDRSLQDGGASQAESKHDELEETKE 1088

Query: 1094 KAGQANAGM-QEFVGRKKFVTRMDSGPEPPERKLFCSMEDKVKSFDQVEDKEHKV-SVQG 1153
            ++ +    +  E  G KK    ++   E  E + + S  +  +S  +  + E  + +   
Sbjct: 1089 ESDEMKTSLGVERNGDKK---ELEHQFECQENETYRSNVEAAESSCRFPNGEEIIGAATN 1148

Query: 1154 ANVRAEKG-SGLESTRPTISDRTQKSGELAHEVHVNQATERKEKIVNTSKGKE----SER 1213
             N++  +G  G ES R ++ +    + ++      N      E+   +S  +E    S+ 
Sbjct: 1149 GNMKENEGEEGEESCRSSMEEEGDATSDIGAATDGNMKENEGEESCRSSMEEEGDATSDI 1208

Query: 1214 VRSEADFENDILRKLEEEREREREREKDRMPIDKIALEPRDRVGAEARERAERAALERMT 1273
             +++A+   + L+K++E RE+ERER+++R+ +++   E R+R  A+A ERA + A+E+  
Sbjct: 1209 SQNKAETVEEHLKKIDETREKERERKQERVMVERAIREARERAFADAMERAGKTAMEKAK 1268

Query: 1274 AEARQRALAEARERLEKACAEAREN-SLAGKATMEARVKAERAAVERATAEARERAAEKA 1333
            A A +R   E   + EK   E  +  S A KA+M+A+++AERAAVERA  E RERA EKA
Sbjct: 1269 AVAHRR---EVPRKSEKGSVEVNDKLSSAEKASMQAKLRAERAAVERAITEVRERAMEKA 1328

Query: 1334 MSDRTSFGVRERMERSVSDKFSASSRNNEVRQKFSSSGQPSLQSQSFGSSIASRYAYYSA 1393
            +S +++    +      S  FS+S      R+  SSSG    +++S G S +S       
Sbjct: 1329 LSGKSA--ASQAKSYGGSKSFSSSGE----RRGSSSSG---TENKSSGPSNSS------- 1388

Query: 1394 YDERNEGVDGESPQRCKARLERHQRTAERAAKALAEKNMRDLLAQREQAERNRLAETLDA 1446
                N+   GE  QRCKAR ERHQRT++RAA+ALAEK +RDL  Q+EQ ERNRLAE LDA
Sbjct: 1389 ----NQTAKGEPIQRCKARSERHQRTSDRAAEALAEKKLRDLKTQKEQTERNRLAEALDA 1448

BLAST of Spg013516 vs. ExPASy Swiss-Prot
Match: Q0WQ57 (Auxilin-related protein 2 OS=Arabidopsis thaliana OX=3702 GN=At4g12770 PE=1 SV=1)

HSP 1 Score: 268.1 bits (684), Expect = 5.9e-70
Identity = 206/485 (42.47%), Postives = 278/485 (57.32%), Query Frame = 0

Query: 1014 VGRKKFVTRMDSGPEP---PERKLFCSMEDKVKSFDQVEDKEHKVSVQGANVRAEKGSGL 1073
            +GR +  T  +  P+P    +  +F +      +     DK  +   + A  R EK S L
Sbjct: 426  IGRNQ--TAANGYPDPSSGEDSDVFSTAAASAAAMKDAMDKA-EAKFRHAKERREKES-L 485

Query: 1074 ESTRPTISDRTQKSGELAHEVHVNQATERKEKIVNTSKGKESERVRSEADFENDILRKLE 1133
            +++R    D T        E + ++  E +EK V   +    ER   EA+ E    R+ E
Sbjct: 486  KASRSREGDHT--------ENYDSRERELREKQVRLDR----ERAEREAEMEKTQARERE 545

Query: 1134 EER------EREREREKDRMPIDKIALEPRDRVGAE---------------ARERAERAA 1193
            E        ERERER   R  +++   E R+R   E               ARERAERAA
Sbjct: 546  EREREQKRIERERERLLARQAVERATREARERAATEAHAKVQRAAVGKVTDARERAERAA 605

Query: 1194 LERMTAEARQRALAEARERLEKACAEARENSLAGKATMEARVKAERAAVERATAEARERA 1253
            ++R  AEAR+RA A ARE+ EKA AEARE + A     EA+V+AERAAVERA AEAR RA
Sbjct: 606  VQRAHAEARERAAAGAREKAEKAAAEARERANAEVREKEAKVRAERAAVERAAAEARGRA 665

Query: 1254 AEKAMSDRTSFGVRERMERSVSDKFSASSRNNEVRQKFSSSGQPSLQSQSFGSS------ 1313
            A +A + +     ++     +   F++ SR + V ++ ++   P   S + G S      
Sbjct: 666  AAQAKAKQ-----QQENNNDLDSFFNSVSRPSSVPRQRTNPPDPFQDSWNKGGSFESSRP 725

Query: 1314 ------------------------IASRYAYYSAYDERNEGVDGESPQRCKARLERHQRT 1373
                                    ++S +   ++     + VDGE+ +R +ARLERHQRT
Sbjct: 726  SSRVPSGPTENLRKASSATNIVDDLSSIFGAPASQSGGFQDVDGETEERRRARLERHQRT 785

Query: 1374 AERAAKALAEKNMRDLLAQREQAERNRLAETLDADVRRWSSGKEGNLRALLSTLQYILGP 1433
             ERAAKALAEKN RDL  QREQAE++R+  TLD ++RRW +GKEGNLRALLSTLQY+L P
Sbjct: 786  QERAAKALAEKNERDLQVQREQAEKDRIGGTLDVEIRRWGAGKEGNLRALLSTLQYVLWP 845

Query: 1434 DSGWQPIPLTEVITAAAVKKAYRKATLCVHPDKLQQRGASIQQKYICEKVFDLLKEAWNK 1445
            + GWQP+ LT++IT A+VKK YRKATLC+HPDK+QQ+GA++QQKYI EKVFD+LKEAWNK
Sbjct: 846  ECGWQPVSLTDLITGASVKKVYRKATLCIHPDKVQQKGANLQQKYIAEKVFDMLKEAWNK 889

BLAST of Spg013516 vs. ExPASy Swiss-Prot
Match: Q9SU08 (Auxilin-related protein 1 OS=Arabidopsis thaliana OX=3702 GN=AUXI1 PE=1 SV=2)

HSP 1 Score: 260.0 bits (663), Expect = 1.6e-67
Identity = 208/493 (42.19%), Postives = 280/493 (56.80%), Query Frame = 0

Query: 1014 VGRKKFVTRMDSGPEP---PERKLFCSMEDKVKSFDQVEDKEHKVSVQGANVRAEKGSGL 1073
            +GR +  T  +  P+P    +  +F +      +     DK  +   + A  R EK   L
Sbjct: 428  IGRNQ--TAANGYPDPSSGEDSDVFSTAAASAAAMKDAMDKA-EAKFRHAKERREK-ENL 487

Query: 1074 ESTRPTISDRTQKSGELAHEVHVNQA---TERKEKIVNTSKGKESERVRSEADFENDILR 1133
            +++R    D T+       E+   Q     ER E+     K +E E+   E +       
Sbjct: 488  KASRSREGDHTENYDSRERELREKQVRLDRERAEREAEMEKAQEREKEERERE------- 547

Query: 1134 KLEEEREREREREKDRMPIDKIALEPRDRVGAE---------------ARERAERAALER 1193
              ++  ERERER   R  +++   E R+R   E               ARERAERAA++R
Sbjct: 548  --QKRIERERERLVARQAVERATREARERAATEAHAKVQRAAVGKATDARERAERAAVQR 607

Query: 1194 MTAEARQR-----------ALAEARERLEKACAEARENSLAGKATMEARVKAERAAVERA 1253
              AEAR+R           A AEARE+ EKA AEA+E + A     E RV+AERAAVERA
Sbjct: 608  AHAEARERAAAGARDKAAKAAAEAREKAEKAAAEAKERANAEAREKETRVRAERAAVERA 667

Query: 1254 TAEARERAAEKAMSDRTSFGVRERMERSVSDKFSASSR-NNEVRQK-------------- 1313
             AEAR RAA +A + +     ++     +   FS+ SR N+  RQ+              
Sbjct: 668  AAEARGRAAAQAKAKQ-----QQENTNDLDSFFSSISRPNSAPRQRTNPLDPFQDSWNKG 727

Query: 1314 --FSSS--------GQP-SLQSQSFGSSI----ASRYAYYSAYDERNEGVDGESPQRCKA 1373
              F SS        GQP +L+  S  ++I    +S +   ++     + VDGE+ +R +A
Sbjct: 728  GSFESSRESLRVPPGQPENLRKTSSVTNIVDDLSSIFGASASQSGGFQDVDGETEERRRA 787

Query: 1374 RLERHQRTAERAAKALAEKNMRDLLAQREQAERNRLAETLDADVRRWSSGKEGNLRALLS 1433
            RLERHQRT ERAAKALAEKN RDL  QREQ E++R+  TLD +++RW +GKEGNLRALLS
Sbjct: 788  RLERHQRTQERAAKALAEKNERDLQVQREQVEKDRIGVTLDVEIKRWGAGKEGNLRALLS 847

Query: 1434 TLQYILGPDSGWQPIPLTEVITAAAVKKAYRKATLCVHPDKLQQRGASIQQKYICEKVFD 1445
            TLQY+L P+ GWQP+ LT++ITAA+VKK YRKATLC+HPDK+QQ+GA++QQKYI EKVFD
Sbjct: 848  TLQYVLWPECGWQPVSLTDLITAASVKKVYRKATLCIHPDKVQQKGANLQQKYIAEKVFD 902

BLAST of Spg013516 vs. ExPASy Swiss-Prot
Match: O13773 (UBA domain-containing protein 7 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=ucp7 PE=4 SV=1)

HSP 1 Score: 87.8 bits (216), Expect = 1.1e-15
Identity = 52/136 (38.24%), Postives = 74/136 (54.41%), Query Frame = 0

Query: 1308 RCKARLERHQRTAERAAKALAEKNMRDLLAQREQAERNRLAETLDADVRRWSSGKEGNLR 1367
            + K+   +H R A++ A+ L E             ER+RL E +   V +W  GKE NLR
Sbjct: 573  KVKSERLQHVRMAQQKAEQLDE-------------ERSRLREPVQQIVNKWKEGKESNLR 632

Query: 1368 ALLSTLQYILGPDSGWQPIPLTEVITAAAVKKAYRKATLCVHPDKLQQRGASIQQKYICE 1427
            ALL++L  IL P+  WQ + L+E++    VK AY KA   VHPDKL Q+  S++ + I E
Sbjct: 633  ALLASLDTILWPECRWQKVSLSELVLPKKVKIAYMKAVSRVHPDKLPQQ-TSVEHQLIAE 692

Query: 1428 KVFDLLKEAWNKFNSE 1444
              F +L  AW  F  +
Sbjct: 693  SAFSILNHAWELFKQQ 694

BLAST of Spg013516 vs. ExPASy Swiss-Prot
Match: Q9C9Q4 (J domain-containing protein required for chloroplast accumulation response 1 OS=Arabidopsis thaliana OX=3702 GN=JAC1 PE=1 SV=1)

HSP 1 Score: 83.2 bits (204), Expect = 2.7e-14
Identity = 112/421 (26.60%), Postives = 184/421 (43.71%), Query Frame = 0

Query: 1051 KEHKVSVQGANVRAEKGSGLESTRPTISDRTQKSGELAHEVHVNQATERKEKIVNTSKGK 1110
            K   V   G N   +  SGL   +       ++ G    E       + ++     SK +
Sbjct: 244  KTSVVEKLGKNEEGDGKSGLSGLKDVKKTSLKRPGVQTKEEKTETDLKSEQAFFGVSKAR 303

Query: 1111 ES-----ERVRSEADF-------ENDILRKLEEEREREREREKDRMPIDKIALEPRDRVG 1170
            E+     + V SE  F       E   ++ L      E ER+      +KI  E   R G
Sbjct: 304  EANVKPLDSVESEQAFSGVSKAHEATTVKPLHSIFHEEDERQD-----EKIVSEREVRKG 363

Query: 1171 AEARERAERAALERMTAEARQRALA---------EARERLEKACAEARENSLAGKATMEA 1230
                +       +  T +  Q   +         ++      A  E  ++ + GK +   
Sbjct: 364  KSKAKNTRSFTEDSRTKKKSQGTKSSLDSSPIPDKSSFASSSAAPEVGKDGVKGKVSDFV 423

Query: 1231 RVKAERAAV----ERATAEARERAAEKAMSDRTSFGVRERMERSVSDKFSASSRNNEVRQ 1290
            ++ ++ A+V    E     +R RA E   +D    G   +   ++ D+   S+ +     
Sbjct: 424  KIFSKGASVGAGGESLGQSSRWRAKETPKTDIIHDGSNAKETVNIPDQQKKSTPDIPAMN 483

Query: 1291 KFSSSGQPSLQSQSFGSSIASRYAYYSAYDERNEGVDGESPQRCKARLERHQRTAERAAK 1350
            +     Q + +  S   S+  +    +  +ER E                H  + +    
Sbjct: 484  RDQKPSQSTQKKDSDRESMNYKAPGDTVQEERQE------------PSTTHTTSEDIDEP 543

Query: 1351 ALAEKNMRDLLA-QREQAERNRLAE---TLDADVRRWSSGKEGNLRALLSTLQYILGPDS 1410
                 ++ D+   + +  E N+ AE    +DA +R+WSSGK GN+R+LLSTLQYIL   S
Sbjct: 544  FHVNFDVEDITQDENKMEEANKDAEEIKNIDAKIRKWSSGKSGNIRSLLSTLQYILWSGS 603

Query: 1411 GWQPIPLTEVITAAAVKKAYRKATLCVHPDKLQQRGASIQQKYICEKVFDLLKEAWNKFN 1443
            GW+P+PL ++I   AV+K+Y++A L +HPDKLQQ+GAS  QKY+ EKVF+LL+EAW+ FN
Sbjct: 604  GWKPVPLMDMIEGNAVRKSYQRALLILHPDKLQQKGASANQKYMAEKVFELLQEAWDHFN 647

BLAST of Spg013516 vs. ExPASy TrEMBL
Match: A0A6J1HGU4 (auxilin-like protein 1 OS=Cucurbita moschata OX=3662 GN=LOC111463353 PE=4 SV=1)

HSP 1 Score: 2170.6 bits (5623), Expect = 0.0e+00
Identity = 1189/1455 (81.72%), Postives = 1280/1455 (87.97%), Query Frame = 0

Query: 1    MDNLSHSRLPNRGSTSVSKKICNGSNGGGPFVAQTIYDDVYGGPPKFGVSALSPRFEDYG 60
            MDNLSHSRLPNRGSTSVSKKICNGSNGGGPFVAQTIYDDVYGGPPKFGVSALSPRFEDYG
Sbjct: 1    MDNLSHSRLPNRGSTSVSKKICNGSNGGGPFVAQTIYDDVYGGPPKFGVSALSPRFEDYG 60

Query: 61   EIFGSFHALRASSIPILDLPAVDESEVFFDARSSAFDYTEVFGGFDGLDFAISYEELVGP 120
            EIFGSFHALR SSIPILDLPAVDESEVFFDARSSAFDY EVFGGFDGL+FAISYEELVGP
Sbjct: 61   EIFGSFHALRTSSIPILDLPAVDESEVFFDARSSAFDYGEVFGGFDGLNFAISYEELVGP 120

Query: 121  SKGVDDGSSDEAWTPAGTESLSDCSDHSGNSHCMSNGDSNQSFGGSTEFCISYNKVNREG 180
            S+GVDDGSSDEAWTPAGT+SLSDCSDHSGNSHCMSN DSNQSFG STEF ISYNKVNREG
Sbjct: 121  SQGVDDGSSDEAWTPAGTDSLSDCSDHSGNSHCMSNEDSNQSFGESTEFSISYNKVNREG 180

Query: 181  NGNMSNGKTHVTQLETLPGFSYLVDEAIPSPKGTDDDPSLQTNDDSYLNIDFDTGKVKGK 240
            NGN+S+ K HVTQLE LPGFSYLVDEA PSPKGT DD SL+TNDDSYLNIDFDTGKVKGK
Sbjct: 181  NGNVSDEKIHVTQLEMLPGFSYLVDEAHPSPKGTYDDLSLKTNDDSYLNIDFDTGKVKGK 240

Query: 241  HPRNTMPPPVDSNGSGQLFEDNHICQNGYGRGGCRSHEDFITVSEISLRTEPSQVPPPSR 300
            HP+NTMPPPVDSNGSG +FED+ ICQN +GR    SHEDFITVSEISLRTEPSQVPPP+R
Sbjct: 241  HPKNTMPPPVDSNGSGNVFEDSLICQNEFGREVFHSHEDFITVSEISLRTEPSQVPPPTR 300

Query: 301  PPPKFAAKKGDYTKMTLNRGEAASEIISDDHSLPLFDVEVDASSSAAASAAAMKEAMVKA 360
            PPPK+AAK GDYTKM LN G+ ASEIISDDH  PLFDVEVDASSSAAASAAAMKEAM KA
Sbjct: 301  PPPKYAAKMGDYTKMALNCGD-ASEIISDDHFNPLFDVEVDASSSAAASAAAMKEAMEKA 360

Query: 361  QAQLQNAKDLWERKKEGVHGRMRLDAKNDMREKEGNLTKIPNRCKSLPNEKVQGIGEIHD 420
            QA+LQ+AKDLWERKKEGVHGRMRLDAK DMREKEGNLTKIPNR KSL +E+VQ IGEIHD
Sbjct: 361  QARLQSAKDLWERKKEGVHGRMRLDAKYDMREKEGNLTKIPNRFKSLTDERVQDIGEIHD 420

Query: 421  YEMNVPTKEERQKALKATEVCSTLYGGEELLTVAEETVPVRSGSRFLVSENYDCCSEWKD 480
            ++MN+P  E+R+K +KA EVCST +GGEELLTV E+TVPVR+GSRFLVS+N+DCCSEWKD
Sbjct: 421  HKMNLPAGEKRKKDVKAAEVCSTPFGGEELLTVDEKTVPVRNGSRFLVSDNHDCCSEWKD 480

Query: 481  ATEFFELARADVSEQEFESVNNDEISNSVIGRMGAETTIEAWENQKDQDKQGKPFHTAHV 540
            ATEFFELARAD+S +EFE ++NDEISN V   + AET IEAWEN KDQDK GKPFHTAH 
Sbjct: 481  ATEFFELARADISGKEFELLDNDEISNFVTEPIVAETIIEAWENDKDQDKDGKPFHTAHA 540

Query: 541  LNEEMKNLENLVHGEEEDKAKLKPNRNGTRQKEQVKLKIPQDVY-LEANNKKFEVKIPQG 600
            LNE +K LEN+VHG+ EDK KLKPN++ TRQKEQVKL   Q V  LEAN++ F V   Q 
Sbjct: 541  LNENVKKLENMVHGKVEDKIKLKPNKDETRQKEQVKLH--QGVCDLEANDRMFGV--AQD 600

Query: 601  FVEVKKQMSGTSELEKHEKPNEFTQLESELKVEQPVSPQDIEQEKKKVVKRKENGNILKE 660
            F+E KK+MS  SELEKHE   EF QL+SELKV+QPVSP DIEQEKKKVVK KENGNILKE
Sbjct: 601  FMEEKKRMSNASELEKHENRFEFKQLDSELKVDQPVSPIDIEQEKKKVVKGKENGNILKE 660

Query: 661  SHRKENNANNLEEAIENEKREKLLAEASEREKLEQKTRMFLEQEENMKRLNLVLEEENFE 720
            SHRKENNAN +EEA+ENEKRE+L +EAS REKLE+K  MFLEQEEN KRLNLVL +ENF 
Sbjct: 661  SHRKENNANKMEEALENEKREELFSEASVREKLEKKKMMFLEQEENKKRLNLVLADENFR 720

Query: 721  GQMSCERQI------EDHGKKEKEAAKVGVSERSKAVHETEDDDKWSKKFQYREECEKGV 780
            GQM+ ER++      EDHGK+ KEAAKV VSERSK  HE EDDDKWSK  QYREECE GV
Sbjct: 721  GQMARERELDGVHGKEDHGKRVKEAAKV-VSERSKLAHEREDDDKWSKNIQYREECEIGV 780

Query: 781  EDSFQHLNVEEMPRDAVNCKVTQMLAEDSQESADLEGTSMEHDEIERLKDRHQVNSTIGS 840
            EDSFQ LN+E  P D   CKV QML EDSQ SADL+G SMEHDE+E+L DRH+VNSTIGS
Sbjct: 781  EDSFQRLNIERRPGDVGKCKVPQMLVEDSQYSADLKGASMEHDELEKLSDRHEVNSTIGS 840

Query: 841  QAYVELEDFGISPAAFQMNDNKNCLPTRLACSTGMPEEFSVVDESEERKIRVTVNDSLPE 900
            QAYVE++D GIS AAFQM+D++N LPT LACS G+PEEFSVVDES ER   VT N +  E
Sbjct: 841  QAYVEVKDLGISAAAFQMHDDQNHLPTVLACSMGVPEEFSVVDESGER-TTVTGNKTSSE 900

Query: 901  SGGIENLQFYKNSCASSMCHAEVEHHKVPVEMEDADIQFSFDELTTKAGKETSFQSKPGH 960
            S GI+NLQF KNS A     AE+EHHKV VEMEDADIQ SF+E T K GKET FQ KPG 
Sbjct: 901  SDGIDNLQFSKNSSALGARQAEIEHHKVSVEMEDADIQLSFNEWTKKVGKETCFQPKPGD 960

Query: 961  TPAEATNSEDAVSFENSTSMDERESEIEENVLKMEDMKTSLPLDITDEKAGQANAGMQEF 1020
            T AEATNSEDA S ENST +DERE EIEEN LKM+DMKTS PLD + E+A Q+N GM+EF
Sbjct: 961  TQAEATNSEDAFSSENSTCVDEREHEIEENALKMDDMKTSPPLDRSGERADQSNVGMEEF 1020

Query: 1021 VGRKKFVTRMDSGPEPPERKLFCSMEDKVKSFDQVEDKEHKVSVQGANVRAEKGSGLEST 1080
            VGRK FVTRMD  PE PE  LFC MEDKVK  ++ ED+   VSVQG N++AEKGSG+EST
Sbjct: 1021 VGRKTFVTRMDCDPEQPESNLFCRMEDKVKFSNEAEDEGQTVSVQGVNIKAEKGSGMEST 1080

Query: 1081 RPTISDRTQKSGELAHEVHVNQATERKEKIVN---TSKGKESERVRSEADFENDILRKLE 1140
            RPTIS RTQKSGE A+EV+ NQATERKEK+VN    SKGKESERVRSE++FEND LRKLE
Sbjct: 1081 RPTISGRTQKSGEFAYEVNANQATERKEKLVNQSHKSKGKESERVRSESEFENDRLRKLE 1140

Query: 1141 EEREREREREKDRMPIDKIALEPRDRVGAEARERAERAALERMTAEARQRALAEARERLE 1200
            EEREREREREKDRM ID+IALEPRDRVGAE RERAER ALERMTAEARQRAL EARERLE
Sbjct: 1141 EEREREREREKDRMHIDRIALEPRDRVGAETRERAERTALERMTAEARQRALTEARERLE 1200

Query: 1201 KACAEARENSLAGKATMEARVKAERAAVERATAEARERAAEKAMSDRTSFGVRERMERSV 1260
            KACAEARENSLAGKATMEARVKAERAAVERATAEARERAAEKAMS+RTSFGVRERMERSV
Sbjct: 1201 KACAEARENSLAGKATMEARVKAERAAVERATAEARERAAEKAMSERTSFGVRERMERSV 1260

Query: 1261 SDKFSASSRNNEVRQKFSSSGQPSLQSQSFGSSIASRYAYYSAYDERNEGVDGESPQRCK 1320
            SDKFSASSRNNE+RQK SSSGQPSLQSQSFGSS+ASRYAY+SAYDERNEGVDGESPQRCK
Sbjct: 1261 SDKFSASSRNNEMRQKSSSSGQPSLQSQSFGSSLASRYAYHSAYDERNEGVDGESPQRCK 1320

Query: 1321 ARLERHQRTAERAAKALAEKNMRDLLAQREQAERNRLAETLDADVRRWSSGKEGNLRALL 1380
            ARLERHQRTAERAAKALAEKNMRDLLAQREQAERNRLAETLDADVRRWSSGKEGNLRALL
Sbjct: 1321 ARLERHQRTAERAAKALAEKNMRDLLAQREQAERNRLAETLDADVRRWSSGKEGNLRALL 1380

Query: 1381 STLQYILGPDSGWQPIPLTEVITAAAVKKAYRKATLCVHPDKLQQRGASIQQKYICEKVF 1440
            STLQYILGP SGWQPIPLTEVITAAAVKKAYRKATLCVHPDKLQQRGASIQQKYICEKVF
Sbjct: 1381 STLQYILGPGSGWQPIPLTEVITAAAVKKAYRKATLCVHPDKLQQRGASIQQKYICEKVF 1440

Query: 1441 DLLKEAWNKFNSEER 1446
            DLLKEAWNKFNSEER
Sbjct: 1441 DLLKEAWNKFNSEER 1448

BLAST of Spg013516 vs. ExPASy TrEMBL
Match: A0A6J1KTC5 (uncharacterized protein LOC111497445 OS=Cucurbita maxima OX=3661 GN=LOC111497445 PE=4 SV=1)

HSP 1 Score: 2165.6 bits (5610), Expect = 0.0e+00
Identity = 1186/1455 (81.51%), Postives = 1278/1455 (87.84%), Query Frame = 0

Query: 1    MDNLSHSRLPNRGSTSVSKKICNGSNGGGPFVAQTIYDDVYGGPPKFGVSALSPRFEDYG 60
            MDNLSHSRLPNRGSTSVSKKICNGSNGGGPF+AQTIYDDVYGGPPKFGVSALSPRFEDYG
Sbjct: 1    MDNLSHSRLPNRGSTSVSKKICNGSNGGGPFIAQTIYDDVYGGPPKFGVSALSPRFEDYG 60

Query: 61   EIFGSFHALRASSIPILDLPAVDESEVFFDARSSAFDYTEVFGGFDGLDFAISYEELVGP 120
            EIFGSFHALR SSIPILDLPAVDESEVFFDARSSAFDY EVFGGFDGL+FAISYEELVGP
Sbjct: 61   EIFGSFHALRTSSIPILDLPAVDESEVFFDARSSAFDYAEVFGGFDGLNFAISYEELVGP 120

Query: 121  SKGVDDGSSDEAWTPAGTESLSDCSDHSGNSHCMSNGDSNQSFGGSTEFCISYNKVNREG 180
            S+GVDDGSSDEAWTPAGT+SLSDCSDHSGNSHCMSN DSNQSFG STEF ISYNKVNREG
Sbjct: 121  SQGVDDGSSDEAWTPAGTDSLSDCSDHSGNSHCMSNEDSNQSFGESTEFSISYNKVNREG 180

Query: 181  NGNMSNGKTHVTQLETLPGFSYLVDEAIPSPKGTDDDPSLQTNDDSYLNIDFDTGKVKGK 240
            NGN+S+GK HVTQLE LPGFSYLVDEA PSPKGT DD SL+TNDDSYLNIDFDTGKVKGK
Sbjct: 181  NGNVSDGKIHVTQLEMLPGFSYLVDEAHPSPKGTYDDLSLKTNDDSYLNIDFDTGKVKGK 240

Query: 241  HPRNTMPPPVDSNGSGQLFEDNHICQNGYGRGGCRSHEDFITVSEISLRTEPSQVPPPSR 300
            HP+NTMPPPVDSNGSG +FED+ ICQN +GR    SHEDFITVSEISLRTEPSQVPPP+R
Sbjct: 241  HPKNTMPPPVDSNGSGHVFEDSLICQNEFGREVFHSHEDFITVSEISLRTEPSQVPPPTR 300

Query: 301  PPPKFAAKKGDYTKMTLNRGEAASEIISDDHSLPLFDVEVDASSSAAASAAAMKEAMVKA 360
            PPPK+AAK GDYTKM LN  E ASEIISDDH  PLF+VEVDA SSAAASAAAMKEAM KA
Sbjct: 301  PPPKYAAKMGDYTKMALN-CEDASEIISDDHFNPLFNVEVDAGSSAAASAAAMKEAMEKA 360

Query: 361  QAQLQNAKDLWERKKEGVHGRMRLDAKNDMREKEGNLTKIPNRCKSLPNEKVQGIGEIHD 420
            QA+LQ+AKDLWERKKEGVHGRMRLDAK DMREKEGNLTKIPNR K   +E+VQGIGEIHD
Sbjct: 361  QARLQSAKDLWERKKEGVHGRMRLDAKYDMREKEGNLTKIPNRFKGFADERVQGIGEIHD 420

Query: 421  YEMNVPTKEERQKALKATEVCSTLYGGEELLTVAEETVPVRSGSRFLVSENYDCCSEWKD 480
            ++MN P  E+RQK +KATEV STL+GGEELLTVAE+TV +R+GSRFLVS+N+DCCSEWKD
Sbjct: 421  HKMNFPAGEKRQKDVKATEVSSTLFGGEELLTVAEKTVSIRNGSRFLVSDNHDCCSEWKD 480

Query: 481  ATEFFELARADVSEQEFESVNNDEISNSVIGRMGAETTIEAWENQKDQDKQGKPFHTAHV 540
            ATEFFELARAD+S +EFE ++NDEISN V G + AET I+AWEN KDQDK GKPFHTAH 
Sbjct: 481  ATEFFELARADISGKEFELLDNDEISNFVTGPIVAETIIQAWENDKDQDKDGKPFHTAHA 540

Query: 541  LNEEMKNLENLVHGEEEDKAKLKPNRNGTRQKEQVKLKIPQDVY-LEANNKKFEVKIPQG 600
            LNE +KNLEN+VHG+ EDK KLKPN++ TRQKEQVKL   Q V  LEAN++ F V   Q 
Sbjct: 541  LNENVKNLENMVHGKVEDKIKLKPNKDETRQKEQVKLH--QGVCDLEANDRIFGV--AQD 600

Query: 601  FVEVKKQMSGTSELEKHEKPNEFTQLESELKVEQPVSPQDIEQEKKKVVKRKENGNILKE 660
            F+E KK+ S  SELEKHE   EF QL+SELKV+QPVSP DIEQEKKKVVK KENGNILKE
Sbjct: 601  FMEEKKRTSNASELEKHENRIEFKQLDSELKVDQPVSPIDIEQEKKKVVKGKENGNILKE 660

Query: 661  SHRKENNANNLEEAIENEKREKLLAEASEREKLEQKTRMFLEQEENMKRLNLVLEEENFE 720
            SHRKENNAN +EEA+ENEKREKL +EASEREKLEQK  MFLEQEEN KRLNLVL +ENF 
Sbjct: 661  SHRKENNANKMEEALENEKREKLFSEASEREKLEQKKMMFLEQEENKKRLNLVLADENFR 720

Query: 721  GQMSCERQI------EDHGKKEKEAAKVGVSERSKAVHETEDDDKWSKKFQYREECEKGV 780
             Q++ E ++      EDHGK+ KEAAKV VSERSK  HE EDDDKWSK  QYREECE GV
Sbjct: 721  RQVAREIELDGVHGKEDHGKRGKEAAKV-VSERSKLAHEREDDDKWSKNIQYREECEIGV 780

Query: 781  EDSFQHLNVEEMPRDAVNCKVTQMLAEDSQESADLEGTSMEHDEIERLKDRHQVNSTIGS 840
            +DSFQ LN+E  P D   CKVTQML EDSQ SADL+G SMEHDE+E+L DRH+VNSTIGS
Sbjct: 781  KDSFQRLNIERRPSDVGKCKVTQMLVEDSQYSADLKGASMEHDELEKLSDRHEVNSTIGS 840

Query: 841  QAYVELEDFGISPAAFQMNDNKNCLPTRLACSTGMPEEFSVVDESEERKIRVTVNDSLPE 900
            QAYVE++D GIS AAFQM+D++N LPT LACS G+PEEFSVVDES  R   VT N +  E
Sbjct: 841  QAYVEVKDLGISAAAFQMHDDQNHLPTVLACSIGVPEEFSVVDESGVR-TTVTGNKTSSE 900

Query: 901  SGGIENLQFYKNSCASSMCHAEVEHHKVPVEMEDADIQFSFDELTTKAGKETSFQSKPGH 960
            S GI+NLQF KNS A     AE+EHHKV VEMEDADIQ SF+E T K GKETSFQ KPG 
Sbjct: 901  SDGIDNLQFSKNSSALGARQAEIEHHKVSVEMEDADIQLSFNEWTKKVGKETSFQPKPGD 960

Query: 961  TPAEATNSEDAVSFENSTSMDERESEIEENVLKMEDMKTSLPLDITDEKAGQANAGMQEF 1020
            T AEATNSEDA S ENST +DERE++IEEN LKM+DMKTS PLD + E+AGQ+N GM+EF
Sbjct: 961  TQAEATNSEDAFSSENSTCVDERENKIEENALKMDDMKTSPPLDRSGERAGQSNVGMEEF 1020

Query: 1021 VGRKKFVTRMDSGPEPPERKLFCSMEDKVKSFDQVEDKEHKVSVQGANVRAEKGSGLEST 1080
            VGRK FVTR D  PE PE  LFC MEDKVK  +Q ED+   VSVQG N++AEKGSG+EST
Sbjct: 1021 VGRKTFVTRRDCDPEQPESNLFCRMEDKVKFSNQAEDEGQTVSVQGVNIKAEKGSGMEST 1080

Query: 1081 RPTISDRTQKSGELAHEVHVNQATERKEKIVN---TSKGKESERVRSEADFENDILRKLE 1140
            RPTIS RTQKSGE A+EV+ NQATERKEK+VN    SKGKESERVRSEA+FEND+LRKLE
Sbjct: 1081 RPTISGRTQKSGEFAYEVNANQATERKEKLVNQSHKSKGKESERVRSEAEFENDMLRKLE 1140

Query: 1141 EEREREREREKDRMPIDKIALEPRDRVGAEARERAERAALERMTAEARQRALAEARERLE 1200
            EEREREREREKDRM ID+IALEPRDRVGAE RERAER ALERMTAEARQRAL EARERLE
Sbjct: 1141 EEREREREREKDRMHIDRIALEPRDRVGAETRERAERTALERMTAEARQRALTEARERLE 1200

Query: 1201 KACAEARENSLAGKATMEARVKAERAAVERATAEARERAAEKAMSDRTSFGVRERMERSV 1260
            KACAEARENSLAGKATMEARVKAERAAVERATAEARERAAEKAMSDRTSFGVRERMERSV
Sbjct: 1201 KACAEARENSLAGKATMEARVKAERAAVERATAEARERAAEKAMSDRTSFGVRERMERSV 1260

Query: 1261 SDKFSASSRNNEVRQKFSSSGQPSLQSQSFGSSIASRYAYYSAYDERNEGVDGESPQRCK 1320
            SDKFSASSRNNE+RQK SSSGQPSLQSQSFGSS+ SRYAY+SAYDERN GVDGESPQRCK
Sbjct: 1261 SDKFSASSRNNEMRQKSSSSGQPSLQSQSFGSSLDSRYAYHSAYDERNGGVDGESPQRCK 1320

Query: 1321 ARLERHQRTAERAAKALAEKNMRDLLAQREQAERNRLAETLDADVRRWSSGKEGNLRALL 1380
            ARLERHQRTA RAAKALAEKNMRDLLAQREQAERNRLAETLDADVRRWSSGKEGNLRALL
Sbjct: 1321 ARLERHQRTAGRAAKALAEKNMRDLLAQREQAERNRLAETLDADVRRWSSGKEGNLRALL 1380

Query: 1381 STLQYILGPDSGWQPIPLTEVITAAAVKKAYRKATLCVHPDKLQQRGASIQQKYICEKVF 1440
            STLQYILGP SGWQPIPLTEVITAAAVKKAYRKATLCVHPDKLQQRGASIQQKYICEKVF
Sbjct: 1381 STLQYILGPGSGWQPIPLTEVITAAAVKKAYRKATLCVHPDKLQQRGASIQQKYICEKVF 1440

Query: 1441 DLLKEAWNKFNSEER 1446
            DLLKEAWNKFNSEER
Sbjct: 1441 DLLKEAWNKFNSEER 1448

BLAST of Spg013516 vs. ExPASy TrEMBL
Match: A0A6J1BTF2 (auxilin-like protein 1 OS=Momordica charantia OX=3673 GN=LOC111005402 PE=4 SV=1)

HSP 1 Score: 2159.4 bits (5594), Expect = 0.0e+00
Identity = 1168/1456 (80.22%), Postives = 1282/1456 (88.05%), Query Frame = 0

Query: 1    MDNLSHSRLPNRGSTSVSKKICNGSNGGGPFVAQTIYDDVYGGPPKFGVSALSPRFEDYG 60
            MDNLSHSRLPNRGSTSVSK ICNGSNGGG FV  TIYDDVYGGPPKFGVSALSPRFEDYG
Sbjct: 1    MDNLSHSRLPNRGSTSVSKNICNGSNGGGAFVPHTIYDDVYGGPPKFGVSALSPRFEDYG 60

Query: 61   EIFGSFHALRASSIPILDLPAVDESEVFFDARSSAFDYTEVFGGFDGLDFAISYEELVGP 120
            EIFGSFHALRASSIPILDLPAVDE+EVFFDARSSAFDY +VFGGFDGLDFAISYEEL+ P
Sbjct: 61   EIFGSFHALRASSIPILDLPAVDETEVFFDARSSAFDYADVFGGFDGLDFAISYEELIAP 120

Query: 121  SKGVDDGSSDEAWTPAGTESLSDCSDHSGNSHCMSNGDSNQSFGGSTEFCISYNKVNREG 180
            SKGVDDGSSDEAWTPAGTESLSDCSDHSGNS C+SNGDSNQSF  STEFCISYNKVNREG
Sbjct: 121  SKGVDDGSSDEAWTPAGTESLSDCSDHSGNSQCISNGDSNQSFSESTEFCISYNKVNREG 180

Query: 181  NGNMSNGKTHVTQLETLPGFSYLVDEAIPSPKGTDDDPSLQTNDDSYLNIDFDTGKVKGK 240
            NGN+SNGKTHVTQLE LPGFSYLVDEA PS  GT DDPSLQTNDDSYLNIDF TGKV+GK
Sbjct: 181  NGNISNGKTHVTQLEMLPGFSYLVDEAQPSAMGTVDDPSLQTNDDSYLNIDFSTGKVRGK 240

Query: 241  HPRNTMPPPVDSNGSGQLFEDNHICQNGYGRGGCRSHEDFITVSEISLRTEPSQVPPPSR 300
            HPRN+MPPP+DSNG GQ F+DN I  NG+GRG CR +EDFITVSEISLRTEPSQVPPPSR
Sbjct: 241  HPRNSMPPPLDSNGCGQSFKDNLISHNGHGRGVCRPYEDFITVSEISLRTEPSQVPPPSR 300

Query: 301  PPPKFAAKKGDYTKMTLNRGEAASEIISDDHSLPLFDVEVDASSSAAASAAAMKEAMVKA 360
            PPP FAA+ GD TKMTLN  EAASE+I D  SLPLFDVEVD+SSSAAASAAAMKEAM KA
Sbjct: 301  PPPMFAAQNGDCTKMTLNCREAASEVILDGPSLPLFDVEVDSSSSAAASAAAMKEAMEKA 360

Query: 361  QAQLQNAKDLWERKKEGVHGRMRLDAKNDMREKEGNLTKIPNRCKSLPNEKVQGIGEIHD 420
            QAQLQNAKDLWERKKEGVHGRMRLDAKNDMR+KEG+LTKIPNRCK+L NEKVQGIGEIHD
Sbjct: 361  QAQLQNAKDLWERKKEGVHGRMRLDAKNDMRDKEGHLTKIPNRCKNLTNEKVQGIGEIHD 420

Query: 421  YEMNVPTKEERQKALKATEVCSTLYGGEELLTVAEETVPVRSGSRFLVSENYDCCSEWKD 480
            +EM VP +EERQK +KATEVCST YGG+E LTVAE+ +P RSGSRFL S+NY CCSEWK+
Sbjct: 421  HEMKVPAREERQKDVKATEVCSTPYGGDEFLTVAEKYLPNRSGSRFLASDNYGCCSEWKE 480

Query: 481  ATEFFELARADVSEQEFESVNNDEISNSVIGRMGAETTIEAWENQKDQDKQGKPFHTAHV 540
            ATEFF LA AD+ E+EFESVNNDEISN VIG+MGAET+ EAWEN+K++DK GKPFH AH+
Sbjct: 481  ATEFFVLAGADMPEKEFESVNNDEISNFVIGQMGAETSTEAWENEKNKDKLGKPFHKAHL 540

Query: 541  LNEEMKNLENLVHGEEEDKAKLKPNRNGTRQKEQVKLKIPQDVY-LEANNKKFEVKIPQG 600
            +N+E+KNLEN+V G+E+DK K KP++N TRQ+E VKLKIPQ V+ LEAN+KKF V   Q 
Sbjct: 541  VNDEVKNLENIVLGKEKDKIKPKPSKNETRQREVVKLKIPQGVFDLEANDKKFGV--AQE 600

Query: 601  FVEVKKQMSGTSELEKHEKPNEFTQLESELKVEQPVSPQDIEQEKKKVVKRKENGNILKE 660
            FVEVK+ MSG S+LEK EKP EF QLESEL+VEQPVSP+DI+++KK  VK KE  NI KE
Sbjct: 601  FVEVKRPMSGASKLEKLEKPIEFKQLESELRVEQPVSPKDIDEKKKNAVKGKEKENIFKE 660

Query: 661  SHRKENNANNLEEAIENEKREKLLAEASEREKLEQKTRMFLEQEENMKRLNLVL-EEENF 720
            SHRKENN N +EE I NEK+EKL  E SEREKLEQK RM  EQ+EN +RLN+ L EEE+ 
Sbjct: 661  SHRKENNENKMEETIGNEKKEKLRTETSEREKLEQKMRMVFEQQENKERLNMALEEEEDL 720

Query: 721  EGQMSCERQI------EDHGKKEKEAAKVGVSERSKAVHETEDDDKWSKKFQYREECEKG 780
            + +M+ ERQ+      EDH KKE EA KVG+SER K  HETE D KW K FQYREECEKG
Sbjct: 721  KERMAGERQLEGVHEMEDHEKKENEAVKVGLSERFKLAHETEVDVKWLKDFQYREECEKG 780

Query: 781  VEDSFQHLNVEEMPRDAVNCKVTQMLAEDSQESADLEGTSMEHDEIERLKDRHQVNSTIG 840
            VED FQ +N+E MPRD   CK TQML +DSQE+A+L+GTSM HDEIERL DR +VN TIG
Sbjct: 781  VEDLFQRVNIEGMPRDVGKCKETQMLVKDSQENAELKGTSMVHDEIERL-DRQKVNPTIG 840

Query: 841  SQAYVELEDFGISPAAFQMNDNKNCLPTRLACSTGMPEEFSVVDESEERKIRVTVNDSLP 900
            +QAY+E+ED GIS AAFQM+D++N LPT L  STG+  EF+VVDES ER+ +V +N+S  
Sbjct: 841  TQAYMEVEDLGISTAAFQMDDDENHLPTILDFSTGVSLEFAVVDESGEREAKVIINESSS 900

Query: 901  ESGGIENLQFYKNSCASSMCHAEVEHHKVPVEMEDADIQFSFDELTTKAGKETSFQSKPG 960
            ESGGIENLQF K+SCASS+  AEVEHHKVPV+MEDA+I    DE T K+GKETSFQ KP 
Sbjct: 901  ESGGIENLQFNKDSCASSLFQAEVEHHKVPVKMEDANIPLPLDEWTKKSGKETSFQPKPD 960

Query: 961  HTPAEATNSEDAVSFENSTSMDERESEIEENVLKMEDMKTSLPLDITDEKAGQANAGMQE 1020
            +T  E TN EDAVS ENSTSMDE E+EIEEN LKMEDMKTSLPLD +DEKAGQA+AGM+E
Sbjct: 961  YTQEEVTNLEDAVSSENSTSMDEGENEIEENELKMEDMKTSLPLDRSDEKAGQADAGMEE 1020

Query: 1021 FVGRKKFVTRMDSGPEPPERKLFCSMEDKVKSFDQVEDKEHKVSVQGANVRAEKGSGLES 1080
            F+GR KFV+R+DS PE PERKLF  MEDK KSF QVE+KE KV+ Q  NVRA+KGSG+E+
Sbjct: 1021 FIGRMKFVSRVDSDPEHPERKLFGCMEDKTKSFHQVEEKEQKVAAQEVNVRADKGSGMET 1080

Query: 1081 TRPTISDRTQKSGELAHEVHVNQATERKEKIVN---TSKGKESERVRSEADFENDILRKL 1140
             +PTIS+R  KS +++HEV+ NQ TERKEKI+N   TSKGKESERVR EA+FEND+LRKL
Sbjct: 1081 AQPTISERIHKSSKVSHEVNANQPTERKEKIINQSHTSKGKESERVRREAEFENDVLRKL 1140

Query: 1141 EEEREREREREKDRMPIDKIALEPRDRVGAEARERAERAALERMTAEARQRALAEARERL 1200
            EEEREREREREKDRMPID+IALEPRDRVGAEARERAERAALERMTAEARQRALA+ARERL
Sbjct: 1141 EEEREREREREKDRMPIDRIALEPRDRVGAEARERAERAALERMTAEARQRALADARERL 1200

Query: 1201 EKACAEARENSLAGKATMEARVKAERAAVERATAEARERAAEKAMSDRTSFGVRERMERS 1260
            EKACAEARENS AGK TMEARVKAERAAVERATAEARERAAEK +SDRTSFGVRER+ERS
Sbjct: 1201 EKACAEARENSFAGKTTMEARVKAERAAVERATAEARERAAEKVISDRTSFGVRERIERS 1260

Query: 1261 VSDKFSASSRNNEVRQKFSSSGQPSLQSQSFGSSIASRYAYYSAYDERNEGVDGESPQRC 1320
            VSDKFSASSRNNE+RQK SSSGQPSLQSQSFGSSIASRY YYSAYDERNEGVDGESPQRC
Sbjct: 1261 VSDKFSASSRNNEMRQKSSSSGQPSLQSQSFGSSIASRYGYYSAYDERNEGVDGESPQRC 1320

Query: 1321 KARLERHQRTAERAAKALAEKNMRDLLAQREQAERNRLAETLDADVRRWSSGKEGNLRAL 1380
            KAR ERHQRTAERAAKALAEKNMRDL+AQREQAERNRLAETLDADVRRWSSGKEGNLRAL
Sbjct: 1321 KARFERHQRTAERAAKALAEKNMRDLVAQREQAERNRLAETLDADVRRWSSGKEGNLRAL 1380

Query: 1381 LSTLQYILGPDSGWQPIPLTEVITAAAVKKAYRKATLCVHPDKLQQRGASIQQKYICEKV 1440
            LSTLQYILGPDSGWQPIPLTEVITAAAVKKAYRKATLCVHPDKLQQRGASIQQKYICEKV
Sbjct: 1381 LSTLQYILGPDSGWQPIPLTEVITAAAVKKAYRKATLCVHPDKLQQRGASIQQKYICEKV 1440

Query: 1441 FDLLKEAWNKFNSEER 1446
            FDLLKEAWNKFNSEER
Sbjct: 1441 FDLLKEAWNKFNSEER 1453

BLAST of Spg013516 vs. ExPASy TrEMBL
Match: A0A6J1GEV1 (auxilin-like protein 1 OS=Cucurbita moschata OX=3662 GN=LOC111453295 PE=4 SV=1)

HSP 1 Score: 2117.8 bits (5486), Expect = 0.0e+00
Identity = 1158/1455 (79.59%), Postives = 1255/1455 (86.25%), Query Frame = 0

Query: 1    MDNLSHSRLPNRGSTSVSKKICNGSNGGGPFVAQTIYDDVYGGPPKFGVSALSPRFEDYG 60
            MDNLSHSRLPNRGSTS+SKKICNG +G G F AQTIYDDVYGGPPKFGVSALSPRFEDYG
Sbjct: 1    MDNLSHSRLPNRGSTSLSKKICNGGHGAGHFAAQTIYDDVYGGPPKFGVSALSPRFEDYG 60

Query: 61   EIFGSFHALRASSIPILDLPAVDESEVFFDARSSAFDYTEVFGGFDGLDFAISYEELVGP 120
            EIFGSFHALRASSIPILDLPAVDESEVFFDARSSAFDY EVFGGFDGLDFAISYEELVGP
Sbjct: 61   EIFGSFHALRASSIPILDLPAVDESEVFFDARSSAFDYAEVFGGFDGLDFAISYEELVGP 120

Query: 121  SKGVDDGSSDEAWTPAGTESLSDCSDHSGNSHCMSNGDSNQSFGGSTEFCISYNKVNREG 180
            S+ VDD SSDEAWTPAGTESLSDCSDHSGNS CMSNGDSNQS  GSTEFCISY+KVN E 
Sbjct: 121  SRRVDDASSDEAWTPAGTESLSDCSDHSGNSRCMSNGDSNQSCDGSTEFCISYSKVNSEN 180

Query: 181  NGNMSNGKTHVTQLETLPGFSYLVDEAIPSPKGTDDDPSLQTNDDSYLNIDFDTGKVKGK 240
             G++SNGK H TQLE LPG SYLVDEA PS KGTD DPSL TNDD+YLNIDFDTGKVKGK
Sbjct: 181  IGHISNGKIHATQLEMLPGHSYLVDEANPSTKGTDGDPSLLTNDDNYLNIDFDTGKVKGK 240

Query: 241  HPRNTMPPPVDSNGSGQLFEDNHICQNGYGRGGCRSHEDFITVSEISLRTEPSQVPPPSR 300
            HPRNT PPPVD NGSG L EDN + QNGYGRG CRSHEDFI+VSEISLRTEPSQVPPPSR
Sbjct: 241  HPRNTRPPPVDGNGSGPLLEDNLVSQNGYGRGVCRSHEDFISVSEISLRTEPSQVPPPSR 300

Query: 301  PPPKFAAKKGDYTKMTLNRGEAASEIISDDHSLPLFDVEVDASSSAAASAAAMKEAMVKA 360
            PPPKFA KKGD TKMTL+ GEAASEIISDD +LPLFDVEVDASSSAA SAAAMK AM KA
Sbjct: 301  PPPKFATKKGDSTKMTLSCGEAASEIISDDRTLPLFDVEVDASSSAAVSAAAMKGAMEKA 360

Query: 361  QAQLQNAKDLWERKKEGVHGRMRLDAKNDMREKEGNLTKIPNRCKSLPNEKVQGIGEIHD 420
             AQL NAKDLWERKKEGVHGRMR DAK+DMREKEGNLTKIPNR KSL +E VQ IGEIHD
Sbjct: 361  LAQLLNAKDLWERKKEGVHGRMRPDAKSDMREKEGNLTKIPNRFKSLADESVQIIGEIHD 420

Query: 421  YEMNVPTKEERQKALKATEVCSTLYGGEELLTVAEETVPVRSGSRFLVSENYDCCSEWKD 480
              MN P +EERQK ++ATEVCST YGGEELLT AE+TVP+RSGSRFLVS+NYDCCSEWKD
Sbjct: 421  NGMNFPAREERQKDVRATEVCSTHYGGEELLTAAEKTVPIRSGSRFLVSDNYDCCSEWKD 480

Query: 481  ATEFFELARADVSEQEFESVNNDEISNSVIGRMGAETTIEAWENQKDQDKQGKPFHTAHV 540
            AT FFELARAD+S +EFES+NND + N V G++  ET  +AWEN KDQDK+GKPFHT+HV
Sbjct: 481  ATGFFELARADISRKEFESMNNDAVPNIVAGQIRVETINKAWENDKDQDKRGKPFHTSHV 540

Query: 541  LNEEMKNLENLVHGEEEDKAKLKPNRNGTRQKEQVKLKIPQDVY-LEANNKKFEVKIPQG 600
            LNEEMKNLEN+VHG+EEDK KLKPN+N TRQKEQVKLKI QDV  LEAN++KF V   Q 
Sbjct: 541  LNEEMKNLENMVHGKEEDKIKLKPNKNETRQKEQVKLKIHQDVCDLEANDRKFGV--AQE 600

Query: 601  FVEVKKQMSGTSELEKHEKPNEFTQLESELKVEQPVSPQDIEQEKKKVVKRKENGNILKE 660
            FVEVK+Q+S  SELEKHEKP EF QL++ LKVEQP+SP+DIEQEKKKVVKRKENGNILK 
Sbjct: 601  FVEVKRQISDASELEKHEKPIEFRQLDTGLKVEQPISPRDIEQEKKKVVKRKENGNILK- 660

Query: 661  SHRKENNANNLEEAIENEKREKLLAEASEREKLEQKTRMFLEQEENMKRLNLVLEEENFE 720
                       E  IENEKRE +  EASEREKLEQK RMFLEQ++N KRL+LVLE++NF+
Sbjct: 661  -----------EPLIENEKRE-MFIEASEREKLEQKMRMFLEQQKNKKRLDLVLEDDNFK 720

Query: 721  GQMSCERQIE------DHGKKEKEAAKVGVSERSKAVHETEDDDKWSKKFQYREECEKGV 780
             QM  ERQ+E      DHGKK KE AKVGVSER +  HE EDDDKW + F YRE C+KGV
Sbjct: 721  EQMIRERQLEGVHEMDDHGKKGKETAKVGVSERPELAHEREDDDKWPQDFPYREVCQKGV 780

Query: 781  EDSFQHLNVEEMPRDAVNCKVTQMLAEDSQESADLEGTSMEHDEIERLKDRHQVNSTIGS 840
            +DSFQ LN+E MPRDA  C+VTQML ++ Q+SA L+G S+EHDE+ERL+DRH+ N+TI S
Sbjct: 781  DDSFQILNIEGMPRDAGKCEVTQMLVKEFQDSAHLKGISLEHDELERLEDRHKGNTTIRS 840

Query: 841  QAYVELEDFGISPAAFQMNDNKNCLPTRLACSTGMPEEFSVVDESEERKIRVTVNDSLPE 900
            Q YVE+ D GIS AA +MND+KN LPT LACSTGM E+FS VDE EE    V+VN++   
Sbjct: 841  QVYVEMNDLGISAAAIKMNDDKNHLPTGLACSTGMHEKFSHVDEGEETMTAVSVNETSSG 900

Query: 901  SGGIENLQFYKNSCASSMCHAEVEHHKVPVEMEDADIQFSFDELTTKAGKETSFQSKPGH 960
            SGG ENL+  KN+C SS   AEVEH KVPVEMEDA+IQ SF+E T +  +ETSFQ K GH
Sbjct: 901  SGGAENLELKKNACVSSAYQAEVEHKKVPVEMEDANIQLSFNEWTKRTVEETSFQPKLGH 960

Query: 961  TPAEATNSEDAVSFENSTSMDERESEIEENVLKMEDMKTSLPLDITDEKAGQANAGMQEF 1020
            T AE TNSED +S E STSMDE ++E EE V+KMEDMKTSLPLD  DEKAGQA+  M+EF
Sbjct: 961  TEAEPTNSEDELSSEKSTSMDEGKNETEECVVKMEDMKTSLPLDKNDEKAGQADVCMEEF 1020

Query: 1021 VGRKKFVTRMDSGPEPPERKLFCSMEDKVKSFDQVEDKEHKVSVQGANVRAEKGSGLEST 1080
            +GRKKFV RM S PE PE  LFC +EDKVKS DQVEDK  KVSVQG NVRA+KGS LES+
Sbjct: 1021 LGRKKFVNRMGSDPEHPESNLFCCLEDKVKSSDQVEDKRQKVSVQGFNVRAKKGSELESS 1080

Query: 1081 RPTISDRTQKSGELAHEVHVNQATERKEKIVN---TSKGKESERVRSEADFENDILRKLE 1140
            RP IS+RTQKSGE   EV+VN ATERKEKI+N   TSKGKESER RSEA+FEND L+KLE
Sbjct: 1081 RPNISERTQKSGEFMREVNVNPATERKEKIINQSHTSKGKESERARSEAEFENDSLQKLE 1140

Query: 1141 EEREREREREKDRMPIDKIALEPRDRVGAEARERAERAALERMTAEARQRALAEARERLE 1200
            EERERE+EREKDRMPID+IALEP DRVGAE RE+AERAALERMTAEARQRAL EARERLE
Sbjct: 1141 EEREREKEREKDRMPIDRIALEPCDRVGAETREKAERAALERMTAEARQRALTEARERLE 1200

Query: 1201 KACAEARENSLAGKATMEARVKAERAAVERATAEARERAAEKAMSDRTSFGVRERMERSV 1260
            KACAEARENSLAGKATMEARVKAERAAVERA AEARERAAEK MSD+TSFGVRERMERSV
Sbjct: 1201 KACAEARENSLAGKATMEARVKAERAAVERAAAEARERAAEKVMSDKTSFGVRERMERSV 1260

Query: 1261 SDKFSASSRNNEVRQKFSSSGQPSLQSQSFGSSIASRYAYYSAYDERNEGVDGESPQRCK 1320
            SDKFSAS+ N E+RQK SSSGQPSLQSQSFGSSIASRYAYYSAYDER EGVD ESPQRCK
Sbjct: 1261 SDKFSASNINIEMRQKSSSSGQPSLQSQSFGSSIASRYAYYSAYDERKEGVDSESPQRCK 1320

Query: 1321 ARLERHQRTAERAAKALAEKNMRDLLAQREQAERNRLAETLDADVRRWSSGKEGNLRALL 1380
            ARLERHQRTAERAAKALAEKNMRDLLAQREQAERNRLA+TLDADVRRWSSGKEG LRALL
Sbjct: 1321 ARLERHQRTAERAAKALAEKNMRDLLAQREQAERNRLADTLDADVRRWSSGKEGKLRALL 1380

Query: 1381 STLQYILGPDSGWQPIPLTEVITAAAVKKAYRKATLCVHPDKLQQRGASIQQKYICEKVF 1440
            STLQYILGPDSGWQPIPLTEVITAAAVKKAYRKATLCVHPDKLQQRGASIQQKYICEKVF
Sbjct: 1381 STLQYILGPDSGWQPIPLTEVITAAAVKKAYRKATLCVHPDKLQQRGASIQQKYICEKVF 1440

Query: 1441 DLLKEAWNKFNSEER 1446
            DLLKEAWNKFNSEER
Sbjct: 1441 DLLKEAWNKFNSEER 1440

BLAST of Spg013516 vs. ExPASy TrEMBL
Match: A0A6J1IML4 (auxilin-like protein 1 OS=Cucurbita maxima OX=3661 GN=LOC111478410 PE=4 SV=1)

HSP 1 Score: 2096.2 bits (5430), Expect = 0.0e+00
Identity = 1144/1455 (78.63%), Postives = 1254/1455 (86.19%), Query Frame = 0

Query: 1    MDNLSHSRLPNRGSTSVSKKICNGSNGGGPFVAQTIYDDVYGGPPKFGVSALSPRFEDYG 60
            MDNLSHSRLPNRGSTS+SKKICNG +G GPF AQTIYDDVYGGPPKFGVSALSPRFEDYG
Sbjct: 1    MDNLSHSRLPNRGSTSLSKKICNGGHGDGPFAAQTIYDDVYGGPPKFGVSALSPRFEDYG 60

Query: 61   EIFGSFHALRASSIPILDLPAVDESEVFFDARSSAFDYTEVFGGFDGLDFAISYEELVGP 120
            EIFGSFHALRASSIPILDLPAVDESEVFFDARSSAFDY EVFGGFDGLDFAISYEELVGP
Sbjct: 61   EIFGSFHALRASSIPILDLPAVDESEVFFDARSSAFDYAEVFGGFDGLDFAISYEELVGP 120

Query: 121  SKGVDDGSSDEAWTPAGTESLSDCSDHSGNSHCMSNGDSNQSFGGSTEFCISYNKVNREG 180
            S+ VDD SSDEAWTPAGTESL DCSDHSGNS CMSNGDSNQS  GSTEFCISY+KVN E 
Sbjct: 121  SRRVDDASSDEAWTPAGTESLLDCSDHSGNSRCMSNGDSNQSCDGSTEFCISYSKVNSEN 180

Query: 181  NGNMSNGKTHVTQLETLPGFSYLVDEAIPSPKGTDDDPSLQTNDDSYLNIDFDTGKVKGK 240
             G++SNGK HVTQLE LPG+SYLVDEA PSPKGTD DPSLQTNDD+YLNIDFDTGKVKGK
Sbjct: 181  IGHISNGKIHVTQLEMLPGYSYLVDEANPSPKGTDGDPSLQTNDDNYLNIDFDTGKVKGK 240

Query: 241  HPRNTMPPPVDSNGSGQLFEDNHICQNGYGRGGCRSHEDFITVSEISLRTEPSQVPPPSR 300
            HPRNT PPPVD NGSG L EDN + QNGYGR  CRSHEDFI+VSEISLRTEPSQVPPPSR
Sbjct: 241  HPRNTRPPPVDGNGSGPLLEDNLVSQNGYGRRVCRSHEDFISVSEISLRTEPSQVPPPSR 300

Query: 301  PPPKFAAKKGDYTKMTLNRGEAASEIISDDHSLPLFDVEVDASSSAAASAAAMKEAMVKA 360
            PPPKFA KKGD TKM L+ GEAASEIISDD +LPLFDVEVDASSSAAASAAAMK AM KA
Sbjct: 301  PPPKFATKKGDSTKMNLSCGEAASEIISDDRTLPLFDVEVDASSSAAASAAAMKGAMKKA 360

Query: 361  QAQLQNAKDLWERKKEGVHGRMRLDAKNDMREKEGNLTKIPNRCKSLPNEKVQGIGEIHD 420
             AQLQNAKDLWE KKEG+HGR+R DAK+DMREKEGNLT+IPNR KSL +E VQ IGEI D
Sbjct: 361  LAQLQNAKDLWESKKEGLHGRIRPDAKSDMREKEGNLTEIPNRFKSLADESVQIIGEIDD 420

Query: 421  YEMNVPTKEERQKALKATEVCSTLYGGEELLTVAEETVPVRSGSRFLVSENYDCCSEWKD 480
              +N P +EERQK ++ATEVCST YGGEELLT AE+TVP+RSGSRFLVS+NYDCCSEWKD
Sbjct: 421  NGINFPAREERQKDVRATEVCSTHYGGEELLTAAEKTVPIRSGSRFLVSDNYDCCSEWKD 480

Query: 481  ATEFFELARADVSEQEFESVNNDEISNSVIGRMGAETTIEAWENQKDQDKQGKPFHTAHV 540
            AT FFELARAD+S +EFE +NND +SN V G++  ET  +AWEN KDQDK+GKPFHT+HV
Sbjct: 481  ATGFFELARADISRKEFEFMNNDAVSNIVAGQIRVETINKAWENDKDQDKKGKPFHTSHV 540

Query: 541  LNEEMKNLENLVHGEEEDKAKLKPNRNGTRQKEQVKLKIPQDVY-LEANNKKFEVKIPQG 600
            LNEE+KNLEN+VHG+EEDK KLKPN+N +RQK+QVKLKI QDV  LEAN++KF V   Q 
Sbjct: 541  LNEEVKNLENMVHGKEEDKIKLKPNKNESRQKQQVKLKIHQDVCDLEANDRKFGV--AQE 600

Query: 601  FVEVKKQMSGTSELEKHEKPNEFTQLESELKVEQPVSPQDIEQEKKKVVKRKENGNILKE 660
            FVEVK+Q+S  SELEKHEKP EF QL+S LKVEQP+SP+DIEQEKKKVVKRK+NGNILK 
Sbjct: 601  FVEVKRQISDASELEKHEKPIEFRQLDSGLKVEQPISPRDIEQEKKKVVKRKKNGNILK- 660

Query: 661  SHRKENNANNLEEAIENEKREKLLAEASEREKLEQKTRMFLEQEENMKRLNLVLEEENFE 720
                       E  IENEKRE +  EAS  EKLEQK RMFLE +EN KRL+ VLE++NF+
Sbjct: 661  -----------EPLIENEKRE-MFIEASGGEKLEQKMRMFLELQENKKRLDSVLEDDNFK 720

Query: 721  GQMSCERQIE------DHGKKEKEAAKVGVSERSKAVHETEDDDKWSKKFQYREECEKGV 780
            GQM+ ERQ+E      DHGK+ KE AKVGVSER +  HE EDDDKW + F YRE C+KGV
Sbjct: 721  GQMTRERQLEGVHEMDDHGKRGKETAKVGVSERPELAHEREDDDKWPQDFPYREVCQKGV 780

Query: 781  EDSFQHLNVEEMPRDAVNCKVTQMLAEDSQESADLEGTSMEHDEIERLKDRHQVNSTIGS 840
            +DSFQ LN+E MPRDA  C+VTQML ++ Q+SA L+G S+EHDE+ERL+DRH+ N+TI S
Sbjct: 781  DDSFQILNIERMPRDAGKCEVTQMLVKEFQDSAHLKGISLEHDELERLEDRHKGNTTIRS 840

Query: 841  QAYVELEDFGISPAAFQMNDNKNCLPTRLACSTGMPEEFSVVDESEERKIRVTVNDSLPE 900
            Q +VE+ D GIS AA +MND+KN LPT LACS GM E+FS VDE EE    VTVN++   
Sbjct: 841  QVHVEMNDLGISAAAIKMNDDKNHLPTGLACSMGMHEKFSDVDEGEETTTAVTVNETSSG 900

Query: 901  SGGIENLQFYKNSCASSMCHAEVEHHKVPVEMEDADIQFSFDELTTKAGKETSFQSKPGH 960
            SGG ENL+  KN+C SS C AE EH+KVPVEMEDA+IQ SF+E T +  +ET+FQ K GH
Sbjct: 901  SGGAENLELNKNACVSSACQAEEEHNKVPVEMEDANIQLSFNEWTKRTVEETNFQPKLGH 960

Query: 961  TPAEATNSEDAVSFENSTSMDERESEIEENVLKMEDMKTSLPLDITDEKAGQANAGMQEF 1020
            T AE TNSED +S E STSMDE ++  EE V+KMEDMKTSLPLD  DEKAGQA+  M+EF
Sbjct: 961  TQAEPTNSEDELSSEKSTSMDEGKNGTEECVVKMEDMKTSLPLDKNDEKAGQADVCMEEF 1020

Query: 1021 VGRKKFVTRMDSGPEPPERKLFCSMEDKVKSFDQVEDKEHKVSVQGANVRAEKGSGLEST 1080
            +GRKKFV RM S  E PE  LFC +EDKVKS DQVEDK  KVSVQG NVRAEKGSGLES+
Sbjct: 1021 LGRKKFVNRMGSDLEHPESNLFCCLEDKVKSSDQVEDKGQKVSVQGFNVRAEKGSGLESS 1080

Query: 1081 RPTISDRTQKSGELAHEVHVNQATERKEKIVN---TSKGKESERVRSEADFENDILRKLE 1140
            RP IS+ TQKSGE   EV+VN ATERKEKI+N   TSKGKESER RS+A+FEND L+KLE
Sbjct: 1081 RPNISESTQKSGEFMREVNVNPATERKEKIINQSCTSKGKESERARSKAEFENDSLQKLE 1140

Query: 1141 EEREREREREKDRMPIDKIALEPRDRVGAEARERAERAALERMTAEARQRALAEARERLE 1200
            EERERE+EREKDRMPID+IALEPRDRVGAE RE+AERAALERMTAEARQRAL EARERL+
Sbjct: 1141 EEREREKEREKDRMPIDRIALEPRDRVGAETREKAERAALERMTAEARQRALTEARERLD 1200

Query: 1201 KACAEARENSLAGKATMEARVKAERAAVERATAEARERAAEKAMSDRTSFGVRERMERSV 1260
            KACAEARENSLAGKATMEARVKAERAAVERATAEARERAAEK MSD+TSF VRERMERSV
Sbjct: 1201 KACAEARENSLAGKATMEARVKAERAAVERATAEARERAAEKVMSDKTSFRVRERMERSV 1260

Query: 1261 SDKFSASSRNNEVRQKFSSSGQPSLQSQSFGSSIASRYAYYSAYDERNEGVDGESPQRCK 1320
            SDKFSAS+ N E+RQK SSSGQPSLQSQS GSSIASRYAYYSAYDER +GVDGESPQRCK
Sbjct: 1261 SDKFSASNINIEMRQKSSSSGQPSLQSQSSGSSIASRYAYYSAYDERKDGVDGESPQRCK 1320

Query: 1321 ARLERHQRTAERAAKALAEKNMRDLLAQREQAERNRLAETLDADVRRWSSGKEGNLRALL 1380
            ARLERHQRTAERAAKALAEKNMRDLLAQREQAERNRLA+TLDADVRRWSSGKEG LRALL
Sbjct: 1321 ARLERHQRTAERAAKALAEKNMRDLLAQREQAERNRLADTLDADVRRWSSGKEGKLRALL 1380

Query: 1381 STLQYILGPDSGWQPIPLTEVITAAAVKKAYRKATLCVHPDKLQQRGASIQQKYICEKVF 1440
            STLQYILGPDSGWQPIPLTEVITAAAVKKAYRKATLCVHPDKLQQRGASIQQKYICEKVF
Sbjct: 1381 STLQYILGPDSGWQPIPLTEVITAAAVKKAYRKATLCVHPDKLQQRGASIQQKYICEKVF 1440

Query: 1441 DLLKEAWNKFNSEER 1446
            DLLKEAWNKFNSEER
Sbjct: 1441 DLLKEAWNKFNSEER 1440

BLAST of Spg013516 vs. TAIR 10
Match: AT4G36520.1 (Chaperone DnaJ-domain superfamily protein )

HSP 1 Score: 570.5 bits (1469), Expect = 3.9e-162
Identity = 553/1574 (35.13%), Postives = 763/1574 (48.48%), Query Frame = 0

Query: 1    MDNLSHSRLPNRGSTSVSKKICNGSNGGGPF----------VAQTIYDDVYGGPPKFGVS 60
            M+NLSHSR PNR S+S+ KK  NG+N  G F             T YDDV+GGPP+FG  
Sbjct: 1    MENLSHSRNPNR-SSSLPKKPYNGNNTTGGFSYNSGKTANTTTTTTYDDVFGGPPRFGAP 60

Query: 61   ALSPRFEDYGEIFGSFHA---LRASSIPILDLPAVDESEVFFDARSSAFDYTEVFGGFDG 120
             LSPR EDY EIF  F+       SSIP+LDLP VD+ +V+FD RS  FDY EVFGGF+ 
Sbjct: 61   TLSPRLEDYCEIFSGFNGSSRAAVSSIPVLDLPLVDDRDVYFDVRSQGFDYREVFGGFND 120

Query: 121  LDFAISYEELVGPSKGV----DDGSSDEAWTPAGTESLSDCSDHSGNSHCMSNG-DSNQS 180
            LD A SYEEL    K      D  SSD+AWTP   ES S  ++HSG S C SNG DS  S
Sbjct: 121  LDLAPSYEELFLQQKSTTVVGDGDSSDDAWTPEEVESCSGGTEHSGKSPCFSNGRDSYDS 180

Query: 181  FGGSTEFCISYNKVNREGNG--NM-SNGKTHVTQLETLPGFSYLVDEAIPSPKGTDDDPS 240
              GSTEF ISYNK ++   G  NM S+G   V  L  +PG++  VD       GT     
Sbjct: 181  IDGSTEFNISYNKASQISGGETNMSSSGVIRVADLGAIPGYTVAVD-------GT----- 240

Query: 241  LQTNDDSYLNIDFDTGKVKGKHPRNTMPPPVDSNGSGQLFEDNHICQNGYGRGGCRSHED 300
                  + LN      KV G       P  V    S  +FED +            S E 
Sbjct: 241  ------TKLN------KVTG-------PSSVGRGTSRFVFEDKYY-----------SSEP 300

Query: 301  FITVSEISLRTEPSQVPPPSRPPPKFAAKKGDYTKMTLNRGEAASEIISDDHSLP-LFDV 360
            F+TVSEI L+T P+ +PPPSR  P     K D+     N     S+   D  S P  FDV
Sbjct: 301  FVTVSEIGLKTHPTGIPPPSRAAP---ILKSDFRSSASNSKTTGSQGSVDSSSSPTFFDV 360

Query: 361  EVDASSSAAASAAAMKEAMVKAQAQLQNAKDLWERKKEGVHGRMRLDAKNDMREKEGNLT 420
            EVDA+S      AA++EAM+KA+A+L++AK+L ERK++                     T
Sbjct: 361  EVDANS------AAVREAMLKAEAKLKSAKELLERKRD-----------------VARTT 420

Query: 421  KIPNRCKSLPNEKVQGIGEIHDYEMNVPTKEERQKALKATEVCSTLYGGEELLTVAEETV 480
              P+  K+   E+ +       Y   +P  +                     + VA +++
Sbjct: 421  STPSSTKNRIKEEGKS-----SYAAGLPVVKSN-------------------IDVARKSL 480

Query: 481  PVRSGSRFLVSE---NYDCCSEWKDA-TEFFELARADVSEQEFESVNNDEISNSVIGRMG 540
              + GS+ L S+     D   EWK+A  ++ EL R ++     E+    ++S  +     
Sbjct: 481  KDKRGSKSLSSQGSATSDGNDEWKEANNQYVELVRTELPRNADENSGGKDVSIPLNAEFF 540

Query: 541  AETTIEA----WENQKDQDKQGKPFHTAHVLNEEMKNLENLVHGEEEDKAKLKPNRNGTR 600
             +    A    WE Q+ + K  +  H A  L +          G  +  ++ K + N   
Sbjct: 541  DQELTWAGNVDWEKQRRRAKGDREDHEARKLPKH--------SGTRKLSSRHKRHENKLA 600

Query: 601  QKEQVKLKIPQDVYLEANN----------KKFEVKIPQGFVEVKKQMSGTSELEKHEKPN 660
            +K   + KI +  ++E  N           +  +K  +  +  +K      EL   EK  
Sbjct: 601  EKAPEEPKIEKSRHVEMGNDLPDHGGIVKHRNLLKPEENKLFTEKPAKQKKELLCEEKTK 660

Query: 661  EFTQLESELKVEQPVSP------QDIEQEKKKVVKRKENGNILKESHRKENNANNLE--- 720
                 + + K  Q  +        D EQ  +K+ +   N + L+ S     N   +E   
Sbjct: 661  RIQNQQLDKKTHQKAAETNQECVYDWEQNARKLREALGNESTLEVSVELNGNGKKMEMRS 720

Query: 721  -------EAIENEKREKLLAEASEREKLEQKTRMFLEQEENMKRLNLVLEEENFEGQMSC 780
                   E ++  + E  + EA  RE+ +++ R+ +E+ EN KRL   LE+E  E ++  
Sbjct: 721  QSETKLNEPLKRMEEETRIKEARLREENDRRERVAVEKAENEKRLKAALEQEEKERKIKE 780

Query: 781  ERQIEDHGKKEKEAAKVGVSERSKAVHETEDDDKWSKKFQYREECEKGVEDSFQHLNVEE 840
             R+  ++ ++  EA +    ER K   + E + +  + F+  EE  +  E        E 
Sbjct: 781  AREKAENERRAVEAREKAEQER-KMKEQQELELQLKEAFEKEEENRRMREAFALEQEKER 840

Query: 841  MPRDAVNCKVTQMLAEDSQESADLE---GTSMEHDEIER-LKDR--HQVNSTIGSQAYVE 900
              ++A   +  +   ++++E A+LE     ++E +E ER +K+R   + N     +   +
Sbjct: 841  RIKEAREKEENERRIKEAREKAELEQRLKATLEQEEKERQIKERQEREENERRAKEVLEQ 900

Query: 901  LEDFGISPAAFQMNDNKNCLPTRLACSTGMPE--EFSVVDESEERKI----RVTVNDSLP 960
             E+      A +  +N+  L           +  E   ++E E+R I    R  +   L 
Sbjct: 901  AENERKLKEALEQKENERRLKETREKEENKKKLREAIELEEKEKRLIEAFERAEIERRLK 960

Query: 961  ESGGIENLQF-YKNSCASSMCHAEVEHHKVPVEMEDADIQFSFDELTTKAG-----KETS 1020
            E    E ++   + +      H E + H+   E E    ++S +E   K       ++T 
Sbjct: 961  EDLEQEEMRMRLQEAKERERLHRENQEHQ---ENERKQHEYSGEESDEKERDACEMEKTC 1020

Query: 1021 FQSKPGHTPAEATNS-----EDAVSFENSTSMDERESEIEENVLKMEDMKT-SLPLDITD 1080
              +K  H    +  S     E+  S +N  S+++++ E EE   + E M   + P  + +
Sbjct: 1021 ETTKEAHGEQSSNESLSDTLEENESIDNDVSVNKQKKE-EEGTRQRESMSAETCPWKVFE 1080

Query: 1081 E--KAGQANAGMQEFVGRKKFVTRMDSGP--------------------EPPERKLFCSM 1140
            +  K      G  E     +   R +  P                       E  +   +
Sbjct: 1081 KTLKDASQKEGTNEMDADTRLFERNEETPRLGENGGCNQQNGESGEESTSVTENIIGGKL 1140

Query: 1141 EDKVKSFDQVEDKEHKVSVQGANVRAEK------GSGLESTRPTISDRTQKS--GELAHE 1200
            E K K+ +  +D      V G     E+      G G +   P  S    K+  G   HE
Sbjct: 1141 EQKSKNSETSKDASVLKRVSGLKTEVEERLEDVVGVGRDQRNPEESKSAPKTSYGFRNHE 1200

Query: 1201 VHVNQATERKE-------------------KIVNTSKGKESERVRSEADFENDILRKLEE 1260
                   ER                       V+  + KE+ER++ E D E + LRK+EE
Sbjct: 1201 YKFTHQQERGNIYETQAGLNQDAKVERPLPSRVSVQREKEAERLKRERDLEMEQLRKVEE 1260

Query: 1261 EREREREREKDRMPIDKIALEPRDRVGAEARERAERAALERMTAEARQRALAEARERLEK 1320
            EREREREREKDRM  D                               QRALA+ARERLEK
Sbjct: 1261 EREREREREKDRMAFD-------------------------------QRALADARERLEK 1320

Query: 1321 ACAEARENSLAGKATMEARVKAERAAVERATAEARERAAEKAMSDRTSFGVRERMERSVS 1380
            ACAEARE SL  K +MEAR++AERAAVERAT+EAR+RAAEKA     +F  RERMERSVS
Sbjct: 1321 ACAEAREKSLPDKLSMEARLRAERAAVERATSEARDRAAEKA-----AFEARERMERSVS 1380

Query: 1381 DKFSASSRNNEVRQKFSSSGQPSLQSQSFGSSIASRYAYYSAYDERNEGVDGESPQRCKA 1440
            DK S SS     R + S S +    S SFG   ASRY       + + G +GESPQR  +
Sbjct: 1381 DKQSQSSGFFGERMEISLSDKQFQNSVSFG---ASRY-------QDSHGTEGESPQRYTS 1422

Query: 1441 RLERHQRTAERAAKALAEKNMRDLLAQREQAERNRLAETLDADVRRWSSGKEGNLRALLS 1446
            RLERHQRTA+R AKALAEKNMRDL+AQREQAER R+AETLD +V+RWSSGKEGN+RALLS
Sbjct: 1441 RLERHQRTADRVAKALAEKNMRDLVAQREQAERIRIAETLDTEVKRWSSGKEGNIRALLS 1422

BLAST of Spg013516 vs. TAIR 10
Match: AT1G75310.1 (auxin-like 1 protein )

HSP 1 Score: 304.7 bits (779), Expect = 4.0e-82
Identity = 439/1532 (28.66%), Postives = 708/1532 (46.21%), Query Frame = 0

Query: 14   STSVSKKICNGSNGGGPFVAQTIYDDVYGGPPKFGVSALSPRFEDYGEIFGSFHALRASS 73
            +T+ S+KI N  +    F A  +YD V+  P    V++ SP   DYGEIF        SS
Sbjct: 9    ATTFSRKISNNRSHSLSFSANAVYDGVFSSP----VNSKSP-LVDYGEIFRG-SGPSPSS 68

Query: 74   IPILDLPAVDESEVFFDARSSAFDYTEVFGGFDGLDFAISYEELVGPSKGVDDGSSDEAW 133
            IP LD+P ++  +V  D RSS  DY+ VFGG    DFA++ +E++  S+     + D+  
Sbjct: 69   IPFLDVPELNVGKVKVDVRSSKLDYSSVFGGLGACDFAVTPKEVIIKSEKKTSINEDKKR 128

Query: 134  TPAGTESLSDCSDHSGNSHCMSNGDSNQSFGGSTEFCISYNKVNREGNGNMSNGKTHVTQ 193
                  + SD           + G  +          ISY++          NG TH+TQ
Sbjct: 129  NRRKGGNSSDVP-------LCNEGKKSPEMVRMKHSDISYHQTVPRN----ENGATHLTQ 188

Query: 194  L-ETLPGFSYLVDEAIPSPKGTDDDPSLQTNDDSYLNIDFDTGKVKGKHPRNTMPPPVD- 253
            +  T+PG         P P    D+ SL    +S          V+ K P N     V  
Sbjct: 189  VPATMPG---------PIPTQVVDNTSLLHKIESK---STPIPAVEKKLPCNEGREEVKA 248

Query: 254  SNGSGQLFEDNHICQNGYGRGGCRSHEDFITVSEISLRTEPSQVPPPSRPPPKFAAKKGD 313
            S   G   E +   +N + R GC + +D  T   +S   E   V PPS        + G 
Sbjct: 249  SRKQGSKTEVDF--ENIFARDGCSTRDD-STCKTVS-NGEYRDVKPPSSFQCTLNGEHGA 308

Query: 314  YTKMT-LNRGEA---ASEIISDDHSLPLFDVEVDASSSAAASAAAMKEAMVKAQAQLQNA 373
              +++ LN G +    +E      S P FD E D +S AA S+AA+K+A+ +AQ ++  A
Sbjct: 309  SERLSGLNSGPSERYETEDADSPSSPPYFDAETDENSVAAESSAALKKAIEEAQIRMNIA 368

Query: 374  KDLWERKKEGVHGRMRLDAKNDMR-EKEGNLTKIPNRCKSLPNEKVQGIGEIHDYEMNVP 433
            K + E+KK G     +L + +D + E +GN TK+    +   +   Q +GE+        
Sbjct: 369  KQMMEKKKSGFRSCAKLKSCDDSKIENKGN-TKVEGITEESRDNNSQILGEMVKPSEQSF 428

Query: 434  TKEERQKALKATEVCSTLYG-GEELLTVAEETVPVRSGSRFLVSENYDCCSEWKDATEFF 493
            + E  Q A +A +    L+G  E LL    +  P     + +++   +   + +   + +
Sbjct: 429  SNEGDQHAKRARK----LWGVPEGLLKSTSDHKPEELEEQDIITLEEE---QARRGRKHW 488

Query: 494  ELARADVSEQEFESVNN---DEISNSVIGRMGAETTIEAWENQKDQDKQGKPFHTAHVLN 553
            EL         F+SV N    E  N    +   +T       Q+ Q     PF+T   L 
Sbjct: 489  ELPGG-----IFKSVMNSKQQEPENLAPAKPEPDT------KQEVQPITENPFYTFGQLG 548

Query: 554  EEMKNLENLVHGEEEDKAKLKPNRNGTRQKEQVKLKIPQDVYLEANNKKFEVKIPQGFVE 613
             ++K +     G    K   K  +  T ++     ++ QD   E+++++    IP     
Sbjct: 549  SKLKCVVEAFTG---SKVSQKDEKQFTEKENSTVTQMVQDE--ESDSQEMLAGIPVIETY 608

Query: 614  VKKQMSGTSELEKHEKPNEFTQLESELKVEQPVSPQDIEQEKKKVVK---RKENGNILKE 673
            +++      + E   + N   + ES +      S Q++E+E    VK   + E+G+ +K+
Sbjct: 609  LREVEETPQQTESKSEMNIEEKSESTICAFTERSSQNMEKETGWQVKSACKFEDGSGVKD 668

Query: 674  SHRKENNANNLEEAIENEKREKLLAEASER--EKLEQKTRMFLEQEENMKRLNLVLEEEN 733
                + N ++    ++ E  +++++E  E      + KT +   +E     LN    +++
Sbjct: 669  F---QENGDHTCNVLDQEGEKEIVSEPQEMLVGPDDSKTYVREVEETPTPSLNKTQSDDS 728

Query: 734  FEGQMSCER-QIEDHGKKEKEAAKVGVSERSKAVHETEDD------------DKWSKKFQ 793
                +S  R  I + G  ++    V    R + V +T +D              W  +  
Sbjct: 729  VGAMVSFNRVNISEPGNIDEVQEAVHKVPRRRRVWKTSEDVYNMIKAPKGNNRPWQLESA 788

Query: 794  YREEC-----EKGVEDSFQHLNVEEMPRDA------VNCKVTQMLAEDSQESADLEGTSM 853
              E       E+GV        +E     A       N  V + +     ++AD +G   
Sbjct: 789  ENETTAMSFHEEGVRIHHASEEIESTSGQASDSGLQENWTVLKQMFRQMFQTADTKGEDE 848

Query: 854  EHDEIERLK---DRHQVNSTIGSQAYVELEDFGISPAAF-QMNDNKNCLPTRLACSTGMP 913
             +  +E  +   D HQ       Q  VE         A  + N++++   T      G  
Sbjct: 849  TYCLVESERGHLDIHQKAQEKYEQVEVETVRTNYEAYAHTRENEDESAQETYCRKEDGKV 908

Query: 914  E---EFSVVDESEERKIRVT------VNDSLPESGGIENLQFYKNSCASSMCHAEVEHH- 973
            E   + S+V E    ++ +       V ++  E+G ++ L         +  HAE+  + 
Sbjct: 909  EVQGKTSLVRELIGEELEMASLEEEDVQEASEEAGWVQGLSELNEINEHADSHAEMLEYD 968

Query: 974  --------------KVPVEMEDADIQFSFDELTTKAGKETSFQSKPGHTPAE-------- 1033
                          +   + E+  I  S D  T++    +SF+ + G +  E        
Sbjct: 969  RSETDSNNSRERFDQTQEQAEETMIDGSIDTDTSR----SSFEMRQGDSYIEEVGIEQHR 1028

Query: 1034 ----------ATNSEDAVSFENSTSMDERESEIEENVLKMEDMKTSL------PLDITDE 1093
                       +N+E+ +   +S S+    S +E++   ++D   S        L+ T E
Sbjct: 1029 SDQFPEKASAVSNTEEHIEEIDSDSIQSGWSVVEDDDRSLQDGGASQAESKHDELEETKE 1088

Query: 1094 KAGQANAGM-QEFVGRKKFVTRMDSGPEPPERKLFCSMEDKVKSFDQVEDKEHKV-SVQG 1153
            ++ +    +  E  G KK    ++   E  E + + S  +  +S  +  + E  + +   
Sbjct: 1089 ESDEMKTSLGVERNGDKK---ELEHQFECQENETYRSNVEAAESSCRFPNGEEIIGAATN 1148

Query: 1154 ANVRAEKG-SGLESTRPTISDRTQKSGELAHEVHVNQATERKEKIVNTSKGKE----SER 1213
             N++  +G  G ES R ++ +    + ++      N      E+   +S  +E    S+ 
Sbjct: 1149 GNMKENEGEEGEESCRSSMEEEGDATSDIGAATDGNMKENEGEESCRSSMEEEGDATSDI 1208

Query: 1214 VRSEADFENDILRKLEEEREREREREKDRMPIDKIALEPRDRVGAEARERAERAALERMT 1273
             +++A+   + L+K++E RE+ERER+++R+ +++   E R+R  A+A ERA + A+E+  
Sbjct: 1209 SQNKAETVEEHLKKIDETREKERERKQERVMVERAIREARERAFADAMERAGKTAMEKAK 1268

Query: 1274 AEARQRALAEARERLEKACAEAREN-SLAGKATMEARVKAERAAVERATAEARERAAEKA 1333
            A A +R   E   + EK   E  +  S A KA+M+A+++AERAAVERA  E RERA EKA
Sbjct: 1269 AVAHRR---EVPRKSEKGSVEVNDKLSSAEKASMQAKLRAERAAVERAITEVRERAMEKA 1328

Query: 1334 MSDRTSFGVRERMERSVSDKFSASSRNNEVRQKFSSSGQPSLQSQSFGSSIASRYAYYSA 1393
            +S +++    +      S  FS+S      R+  SSSG    +++S G S +S       
Sbjct: 1329 LSGKSA--ASQAKSYGGSKSFSSSGE----RRGSSSSG---TENKSSGPSNSSNQT---- 1388

Query: 1394 YDERNEGVDGESPQRCKARLERHQRTAERAAKALAEKNMRDLLAQREQAERNRLAETLDA 1446
                     GE  QRCKAR ERHQRT++RAA+ALAEK +RDL  Q+EQ ERNRLAE LDA
Sbjct: 1389 ---------GEPIQRCKARSERHQRTSDRAAEALAEKKLRDLKTQKEQTERNRLAEALDA 1448

BLAST of Spg013516 vs. TAIR 10
Match: AT4G12770.1 (Chaperone DnaJ-domain superfamily protein )

HSP 1 Score: 268.1 bits (684), Expect = 4.2e-71
Identity = 206/485 (42.47%), Postives = 278/485 (57.32%), Query Frame = 0

Query: 1014 VGRKKFVTRMDSGPEP---PERKLFCSMEDKVKSFDQVEDKEHKVSVQGANVRAEKGSGL 1073
            +GR +  T  +  P+P    +  +F +      +     DK  +   + A  R EK S L
Sbjct: 426  IGRNQ--TAANGYPDPSSGEDSDVFSTAAASAAAMKDAMDKA-EAKFRHAKERREKES-L 485

Query: 1074 ESTRPTISDRTQKSGELAHEVHVNQATERKEKIVNTSKGKESERVRSEADFENDILRKLE 1133
            +++R    D T        E + ++  E +EK V   +    ER   EA+ E    R+ E
Sbjct: 486  KASRSREGDHT--------ENYDSRERELREKQVRLDR----ERAEREAEMEKTQARERE 545

Query: 1134 EER------EREREREKDRMPIDKIALEPRDRVGAE---------------ARERAERAA 1193
            E        ERERER   R  +++   E R+R   E               ARERAERAA
Sbjct: 546  EREREQKRIERERERLLARQAVERATREARERAATEAHAKVQRAAVGKVTDARERAERAA 605

Query: 1194 LERMTAEARQRALAEARERLEKACAEARENSLAGKATMEARVKAERAAVERATAEARERA 1253
            ++R  AEAR+RA A ARE+ EKA AEARE + A     EA+V+AERAAVERA AEAR RA
Sbjct: 606  VQRAHAEARERAAAGAREKAEKAAAEARERANAEVREKEAKVRAERAAVERAAAEARGRA 665

Query: 1254 AEKAMSDRTSFGVRERMERSVSDKFSASSRNNEVRQKFSSSGQPSLQSQSFGSS------ 1313
            A +A + +     ++     +   F++ SR + V ++ ++   P   S + G S      
Sbjct: 666  AAQAKAKQ-----QQENNNDLDSFFNSVSRPSSVPRQRTNPPDPFQDSWNKGGSFESSRP 725

Query: 1314 ------------------------IASRYAYYSAYDERNEGVDGESPQRCKARLERHQRT 1373
                                    ++S +   ++     + VDGE+ +R +ARLERHQRT
Sbjct: 726  SSRVPSGPTENLRKASSATNIVDDLSSIFGAPASQSGGFQDVDGETEERRRARLERHQRT 785

Query: 1374 AERAAKALAEKNMRDLLAQREQAERNRLAETLDADVRRWSSGKEGNLRALLSTLQYILGP 1433
             ERAAKALAEKN RDL  QREQAE++R+  TLD ++RRW +GKEGNLRALLSTLQY+L P
Sbjct: 786  QERAAKALAEKNERDLQVQREQAEKDRIGGTLDVEIRRWGAGKEGNLRALLSTLQYVLWP 845

Query: 1434 DSGWQPIPLTEVITAAAVKKAYRKATLCVHPDKLQQRGASIQQKYICEKVFDLLKEAWNK 1445
            + GWQP+ LT++IT A+VKK YRKATLC+HPDK+QQ+GA++QQKYI EKVFD+LKEAWNK
Sbjct: 846  ECGWQPVSLTDLITGASVKKVYRKATLCIHPDKVQQKGANLQQKYIAEKVFDMLKEAWNK 889

BLAST of Spg013516 vs. TAIR 10
Match: AT4G12770.2 (Chaperone DnaJ-domain superfamily protein )

HSP 1 Score: 267.7 bits (683), Expect = 5.4e-71
Identity = 206/483 (42.65%), Postives = 276/483 (57.14%), Query Frame = 0

Query: 1014 VGRKKFVTRMDSGPEP---PERKLFCSMEDKVKSFDQVEDKEHKVSVQGANVRAEKGSGL 1073
            +GR +  T  +  P+P    +  +F +      +     DK  +   + A  R EK S L
Sbjct: 426  IGRNQ--TAANGYPDPSSGEDSDVFSTAAASAAAMKDAMDKA-EAKFRHAKERREKES-L 485

Query: 1074 ESTRPTISDRTQKSGELAHEVHVNQATERKEKIVNTSKGKESERVRSEADFENDILRKLE 1133
            +++R    D T        E + ++  E +EK V   +    ER   EA+ E    R+ E
Sbjct: 486  KASRSREGDHT--------ENYDSRERELREKQVRLDR----ERAEREAEMEKTQARERE 545

Query: 1134 EER------EREREREKDRMPIDKIALEPRDRVGAE---------------ARERAERAA 1193
            E        ERERER   R  +++   E R+R   E               ARERAERAA
Sbjct: 546  EREREQKRIERERERLLARQAVERATREARERAATEAHAKVQRAAVGKVTDARERAERAA 605

Query: 1194 LERMTAEARQRALAEARERLEKACAEARENSLAGKATMEARVKAERAAVERATAEARERA 1253
            ++R  AEAR+RA A ARE+ EKA AEARE + A     EA+V+AERAAVERA AEAR RA
Sbjct: 606  VQRAHAEARERAAAGAREKAEKAAAEARERANAEVREKEAKVRAERAAVERAAAEARGRA 665

Query: 1254 AEKAMSDRTSFGVRERMERSVSDKFSASSRNNEVRQKFSSSGQPSLQSQSFGSSIAS--- 1313
            A +A + +     ++     +   F++ SR + V ++ ++   P   S + G S  S   
Sbjct: 666  AAQAKAKQ-----QQENNNDLDSFFNSVSRPSSVPRQRTNPPDPFQDSWNKGGSFESSRP 725

Query: 1314 -------------------------RYAYYSAYDERNEGVDGESPQRCKARLERHQRTAE 1373
                                        + ++     + VDGE+ +R +ARLERHQRT E
Sbjct: 726  SSRVPSGPTENLRKASSATNIVDDLSSIFGASQSGGFQDVDGETEERRRARLERHQRTQE 785

Query: 1374 RAAKALAEKNMRDLLAQREQAERNRLAETLDADVRRWSSGKEGNLRALLSTLQYILGPDS 1433
            RAAKALAEKN RDL  QREQAE++R+  TLD ++RRW +GKEGNLRALLSTLQY+L P+ 
Sbjct: 786  RAAKALAEKNERDLQVQREQAEKDRIGGTLDVEIRRWGAGKEGNLRALLSTLQYVLWPEC 845

Query: 1434 GWQPIPLTEVITAAAVKKAYRKATLCVHPDKLQQRGASIQQKYICEKVFDLLKEAWNKFN 1445
            GWQP+ LT++IT A+VKK YRKATLC+HPDK+QQ+GA++QQKYI EKVFD+LKEAWNKFN
Sbjct: 846  GWQPVSLTDLITGASVKKVYRKATLCIHPDKVQQKGANLQQKYIAEKVFDMLKEAWNKFN 887

BLAST of Spg013516 vs. TAIR 10
Match: AT4G12780.1 (Chaperone DnaJ-domain superfamily protein )

HSP 1 Score: 260.0 bits (663), Expect = 1.1e-68
Identity = 208/493 (42.19%), Postives = 280/493 (56.80%), Query Frame = 0

Query: 1014 VGRKKFVTRMDSGPEP---PERKLFCSMEDKVKSFDQVEDKEHKVSVQGANVRAEKGSGL 1073
            +GR +  T  +  P+P    +  +F +      +     DK  +   + A  R EK   L
Sbjct: 428  IGRNQ--TAANGYPDPSSGEDSDVFSTAAASAAAMKDAMDKA-EAKFRHAKERREK-ENL 487

Query: 1074 ESTRPTISDRTQKSGELAHEVHVNQA---TERKEKIVNTSKGKESERVRSEADFENDILR 1133
            +++R    D T+       E+   Q     ER E+     K +E E+   E +       
Sbjct: 488  KASRSREGDHTENYDSRERELREKQVRLDRERAEREAEMEKAQEREKEERERE------- 547

Query: 1134 KLEEEREREREREKDRMPIDKIALEPRDRVGAE---------------ARERAERAALER 1193
              ++  ERERER   R  +++   E R+R   E               ARERAERAA++R
Sbjct: 548  --QKRIERERERLVARQAVERATREARERAATEAHAKVQRAAVGKATDARERAERAAVQR 607

Query: 1194 MTAEARQR-----------ALAEARERLEKACAEARENSLAGKATMEARVKAERAAVERA 1253
              AEAR+R           A AEARE+ EKA AEA+E + A     E RV+AERAAVERA
Sbjct: 608  AHAEARERAAAGARDKAAKAAAEAREKAEKAAAEAKERANAEAREKETRVRAERAAVERA 667

Query: 1254 TAEARERAAEKAMSDRTSFGVRERMERSVSDKFSASSR-NNEVRQK-------------- 1313
             AEAR RAA +A + +     ++     +   FS+ SR N+  RQ+              
Sbjct: 668  AAEARGRAAAQAKAKQ-----QQENTNDLDSFFSSISRPNSAPRQRTNPLDPFQDSWNKG 727

Query: 1314 --FSSS--------GQP-SLQSQSFGSSI----ASRYAYYSAYDERNEGVDGESPQRCKA 1373
              F SS        GQP +L+  S  ++I    +S +   ++     + VDGE+ +R +A
Sbjct: 728  GSFESSRESLRVPPGQPENLRKTSSVTNIVDDLSSIFGASASQSGGFQDVDGETEERRRA 787

Query: 1374 RLERHQRTAERAAKALAEKNMRDLLAQREQAERNRLAETLDADVRRWSSGKEGNLRALLS 1433
            RLERHQRT ERAAKALAEKN RDL  QREQ E++R+  TLD +++RW +GKEGNLRALLS
Sbjct: 788  RLERHQRTQERAAKALAEKNERDLQVQREQVEKDRIGVTLDVEIKRWGAGKEGNLRALLS 847

Query: 1434 TLQYILGPDSGWQPIPLTEVITAAAVKKAYRKATLCVHPDKLQQRGASIQQKYICEKVFD 1445
            TLQY+L P+ GWQP+ LT++ITAA+VKK YRKATLC+HPDK+QQ+GA++QQKYI EKVFD
Sbjct: 848  TLQYVLWPECGWQPVSLTDLITAASVKKVYRKATLCIHPDKVQQKGANLQQKYIAEKVFD 902