Spg013484 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg013484 vs. NCBI nr
Match: XP_038882989.1 (IST1-like protein [Benincasa hispida])

HSP 1 Score: 728.0 bits (1878), Expect = 4.7e-206
Identity = 388/430 (90.23%), Postives = 406/430 (94.42%), Query Frame = 0

Query: 1   MSAATEAAARSVKIVKKFVSLLRRGFNSSKCKTAAKMAVARIKLLRNKREAVVKQMRRDI 60
           MSAATEAAARSVKIVK+F++LLRRGFNSSKCKTAAKMAVARIKLLRNKREAVVKQMRRDI
Sbjct: 1   MSAATEAAARSVKIVKQFITLLRRGFNSSKCKTAAKMAVARIKLLRNKREAVVKQMRRDI 60

Query: 61  ALLLQSGQDATARIRVEHVIREQNVLAANEIIELFCELVVARLSIIAKQRECPADLKEGV 120
           ALLLQSGQDATARIRVEHVIREQNVLAANEIIELFCELVVARLSIIAKQR+CPADLKEGV
Sbjct: 61  ALLLQSGQDATARIRVEHVIREQNVLAANEIIELFCELVVARLSIIAKQRQCPADLKEGV 120

Query: 121 ASLIFAAPRCSEIPELSALRNVFEKKYGKDFVSAATDLRPNCGVNRLLIDKLSVRTPSGQ 180
           ASLIFAAPRCSEIPELSALRNVFEKKYGKDFVSAA DLRPNCGVNRLLIDKLSVRTP+GQ
Sbjct: 121 ASLIFAAPRCSEIPELSALRNVFEKKYGKDFVSAAIDLRPNCGVNRLLIDKLSVRTPTGQ 180

Query: 181 VKLKIMKEIAKEHQIEWDTTESEKELLKPPEELIEGPRTFVSASSLPVKPAASQPAGDNA 240
           VKLKIMKEIAKEH+IEWDTTESEKELLKPPEELIEGPRTFVSA+SLPVKP  S  AGDNA
Sbjct: 181 VKLKIMKEIAKEHKIEWDTTESEKELLKPPEELIEGPRTFVSAASLPVKPVVSHSAGDNA 240

Query: 241 QIGRQAKEYQMTNSREDESMHFQDTASAAEAAAKAAKQAIAAAQAAAYLANKDSNQDARD 300
           Q  R        NSREDESMHFQD+ASAAEAAAKAAKQAIAAA+AAAYLANKD N+DA+D
Sbjct: 241 QNER------TRNSREDESMHFQDSASAAEAAAKAAKQAIAAAEAAAYLANKDLNRDAQD 300

Query: 301 SGFDLGFGVPPANSTPTSSHNMDNQFKAGEERTTPPQSFGRCSSLKNDETRNVNTDYEMA 360
           SGF LGFG  PANSTPT+SHNMD++FK GEERTTP QSFGRCSSLKN++T NVNTDYEMA
Sbjct: 301 SGFGLGFGGSPANSTPTNSHNMDHRFKTGEERTTPTQSFGRCSSLKNEDTGNVNTDYEMA 360

Query: 361 YRRHSYNPTDIKFDESDCEEETQLDDGAGGGVDRPPDRNPPPVPSSRVHPKLPDYDTLAA 420
           YRRHSYNPTDIKFDESDCEEETQ+D+GAG GV RPPDRNPPPVPSSRVHPKLPDYDTLAA
Sbjct: 361 YRRHSYNPTDIKFDESDCEEETQMDEGAGRGVSRPPDRNPPPVPSSRVHPKLPDYDTLAA 420

Query: 421 RFEALKYRKT 431
           RFEALKYRKT
Sbjct: 421 RFEALKYRKT 424

BLAST of Spg013484 vs. NCBI nr
Match: XP_008440890.1 (PREDICTED: IST1-like protein [Cucumis melo] >XP_008440891.1 PREDICTED: IST1-like protein [Cucumis melo] >KAA0025625.1 IST1-like protein [Cucumis melo var. makuwa] >TYK12500.1 IST1-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 699.1 bits (1803), Expect = 2.3e-197
Identity = 376/430 (87.44%), Postives = 397/430 (92.33%), Query Frame = 0

Query: 1   MSAATEAAARSVKIVKKFVSLLRRGFNSSKCKTAAKMAVARIKLLRNKREAVVKQMRRDI 60
           MSAATEAAARS+KIVK+F+++LR GFNSSKCKTAAKMAVARIKLLRNKREAVVKQMRRDI
Sbjct: 1   MSAATEAAARSLKIVKQFITILRCGFNSSKCKTAAKMAVARIKLLRNKREAVVKQMRRDI 60

Query: 61  ALLLQSGQDATARIRVEHVIREQNVLAANEIIELFCELVVARLSIIAKQRECPADLKEGV 120
           ALLLQSGQDATARIRVEHVIREQNVLAANE+IELFCELVVARLSIIAKQR+CPADLKEGV
Sbjct: 61  ALLLQSGQDATARIRVEHVIREQNVLAANEVIELFCELVVARLSIIAKQRQCPADLKEGV 120

Query: 121 ASLIFAAPRCSEIPELSALRNVFEKKYGKDFVSAATDLRPNCGVNRLLIDKLSVRTPSGQ 180
           ASLIFAAPRCSEIPELSALRNVFEKKYGKDFVSAA DLRPNCGVNRLLIDKLSVRTP+G+
Sbjct: 121 ASLIFAAPRCSEIPELSALRNVFEKKYGKDFVSAAVDLRPNCGVNRLLIDKLSVRTPTGE 180

Query: 181 VKLKIMKEIAKEHQIEWDTTESEKELLKPPEELIEGPRTFVSASSLPVKPAASQPAGDNA 240
           VKLKIMKEIAKEH+IEWDTTESEKELLKPPEELIEGPRTFVSA+SLPVKP  S    DNA
Sbjct: 181 VKLKIMKEIAKEHKIEWDTTESEKELLKPPEELIEGPRTFVSAASLPVKPIVSH--SDNA 240

Query: 241 QIGRQAKEYQMTNSREDESMHFQDTASAAEAAAKAAKQAIAAAQAAAYLANKDSNQDARD 300
           QI R       T + E+ SMHFQDTASAAEAAAKAAKQAIAAA+AAAYLANKD NQDARD
Sbjct: 241 QIER-------TTNCENGSMHFQDTASAAEAAAKAAKQAIAAAEAAAYLANKDRNQDARD 300

Query: 301 SGFDLGFGVPPANSTPTSSHNMDNQFKAGEERTTPPQSFGRCSSLKNDETRNVNTDYEMA 360
           SGF+LGFG  PANST   S+NMD+QFKAGE+ T P QSFGRC SLKN+ETRNVNTDYEMA
Sbjct: 301 SGFNLGFGGSPANSTSNRSYNMDHQFKAGEDGTAPTQSFGRCFSLKNEETRNVNTDYEMA 360

Query: 361 YRRHSYNPTDIKFDESDCEEETQLDDGAGGGVDRPPDRNPPPVPSSRVHPKLPDYDTLAA 420
           YRRHSYNPTDIKFDESDCEEET++DD AG GV RPPDRNPPP PSSRVHPKLPDYDTLAA
Sbjct: 361 YRRHSYNPTDIKFDESDCEEETEMDDEAGRGVSRPPDRNPPPAPSSRVHPKLPDYDTLAA 420

Query: 421 RFEALKYRKT 431
           RFEALKYRKT
Sbjct: 421 RFEALKYRKT 421

BLAST of Spg013484 vs. NCBI nr
Match: XP_031743525.1 (IST1-like protein isoform X1 [Cucumis sativus])

HSP 1 Score: 698.0 bits (1800), Expect = 5.2e-197
Identity = 377/430 (87.67%), Postives = 401/430 (93.26%), Query Frame = 0

Query: 1   MSAATEAAARSVKIVKKFVSLLRRGFNSSKCKTAAKMAVARIKLLRNKREAVVKQMRRDI 60
           MSAATEAAARS+KIVK+F+++LR GFNSSKCKTAAKMAVARIKLLRNKREAVVKQMRRDI
Sbjct: 1   MSAATEAAARSLKIVKQFITILRCGFNSSKCKTAAKMAVARIKLLRNKREAVVKQMRRDI 60

Query: 61  ALLLQSGQDATARIRVEHVIREQNVLAANEIIELFCELVVARLSIIAKQRECPADLKEGV 120
           ALLLQSGQDATARIRVEHVIREQNVLAANEIIELFCELVVARLSIIAKQR+CPADLKEGV
Sbjct: 61  ALLLQSGQDATARIRVEHVIREQNVLAANEIIELFCELVVARLSIIAKQRQCPADLKEGV 120

Query: 121 ASLIFAAPRCSEIPELSALRNVFEKKYGKDFVSAATDLRPNCGVNRLLIDKLSVRTPSGQ 180
           ASLIFAAPRCSEIPELSALRNVFEKKYGKDFVSAA DLRPNCGVNRLLIDKLSVRTP+G+
Sbjct: 121 ASLIFAAPRCSEIPELSALRNVFEKKYGKDFVSAAVDLRPNCGVNRLLIDKLSVRTPTGE 180

Query: 181 VKLKIMKEIAKEHQIEWDTTESEKELLKPPEELIEGPRTFVSASSLPVKPAASQPAGDNA 240
           VKLKIMKEIAKEH+IEWDTTESEKELLKP EELIEGPRTFVSA+SLPVKP  S    DNA
Sbjct: 181 VKLKIMKEIAKEHKIEWDTTESEKELLKPSEELIEGPRTFVSAASLPVKPIVSH--SDNA 240

Query: 241 QIGRQAKEYQMTNSREDESMHFQDTASAAEAAAKAAKQAIAAAQAAAYLANKDSNQDARD 300
           QI     E + TNSRE+ESMHFQD+ASAAEAAAKAAKQAIAAA+AAAYLANKD N+DA D
Sbjct: 241 QI-----ESRTTNSRENESMHFQDSASAAEAAAKAAKQAIAAAEAAAYLANKDLNRDAGD 300

Query: 301 SGFDLGF-GVPPANSTPTSSHNMDNQFKAGEERTTPPQSFGRCSSLKNDETRNVNTDYEM 360
           SGF+LGF G PPANSTPT S+NM++QFKAGE+ T P QSFGRCSSLKN+ETRNVNTDYEM
Sbjct: 301 SGFNLGFGGGPPANSTPTRSYNMNHQFKAGEDGTAPTQSFGRCSSLKNEETRNVNTDYEM 360

Query: 361 AYRRHSYNPTDIKFDESDCEEETQLDDGAGGGVDRPPDRNPPPVPSSRVHPKLPDYDTLA 420
           AYRRHSYNPTDIKFDESDCEEET+++D A  GV RPPDRNPPP PSSRVHPKLPDYDTLA
Sbjct: 361 AYRRHSYNPTDIKFDESDCEEETEMEDEADRGVSRPPDRNPPPAPSSRVHPKLPDYDTLA 420

Query: 421 ARFEALKYRK 430
           ARFEALKYRK
Sbjct: 421 ARFEALKYRK 423

BLAST of Spg013484 vs. NCBI nr
Match: XP_004134834.1 (IST1-like protein isoform X2 [Cucumis sativus] >KGN48972.1 hypothetical protein Csa_004187 [Cucumis sativus])

HSP 1 Score: 697.6 bits (1799), Expect = 6.8e-197
Identity = 377/430 (87.67%), Postives = 400/430 (93.02%), Query Frame = 0

Query: 1   MSAATEAAARSVKIVKKFVSLLRRGFNSSKCKTAAKMAVARIKLLRNKREAVVKQMRRDI 60
           MSAATEAAARS+KIVK+F+++LR GFNSSKCKTAAKMAVARIKLLRNKREAVVKQMRRDI
Sbjct: 1   MSAATEAAARSLKIVKQFITILRCGFNSSKCKTAAKMAVARIKLLRNKREAVVKQMRRDI 60

Query: 61  ALLLQSGQDATARIRVEHVIREQNVLAANEIIELFCELVVARLSIIAKQRECPADLKEGV 120
           ALLLQSGQDATARIRVEHVIREQNVLAANEIIELFCELVVARLSIIAKQR+CPADLKEGV
Sbjct: 61  ALLLQSGQDATARIRVEHVIREQNVLAANEIIELFCELVVARLSIIAKQRQCPADLKEGV 120

Query: 121 ASLIFAAPRCSEIPELSALRNVFEKKYGKDFVSAATDLRPNCGVNRLLIDKLSVRTPSGQ 180
           ASLIFAAPRCSEIPELSALRNVFEKKYGKDFVSAA DLRPNCGVNRLLIDKLSVRTP+G+
Sbjct: 121 ASLIFAAPRCSEIPELSALRNVFEKKYGKDFVSAAVDLRPNCGVNRLLIDKLSVRTPTGE 180

Query: 181 VKLKIMKEIAKEHQIEWDTTESEKELLKPPEELIEGPRTFVSASSLPVKPAASQPAGDNA 240
           VKLKIMKEIAKEH+IEWDTTESEKELLKP EELIEGPRTFVSA+SLPVKP  S    DNA
Sbjct: 181 VKLKIMKEIAKEHKIEWDTTESEKELLKPSEELIEGPRTFVSAASLPVKPIVSH--SDNA 240

Query: 241 QIGRQAKEYQMTNSREDESMHFQDTASAAEAAAKAAKQAIAAAQAAAYLANKDSNQDARD 300
           QI R       TNSRE+ESMHFQD+ASAAEAAAKAAKQAIAAA+AAAYLANKD N+DA D
Sbjct: 241 QIER------TTNSRENESMHFQDSASAAEAAAKAAKQAIAAAEAAAYLANKDLNRDAGD 300

Query: 301 SGFDLGF-GVPPANSTPTSSHNMDNQFKAGEERTTPPQSFGRCSSLKNDETRNVNTDYEM 360
           SGF+LGF G PPANSTPT S+NM++QFKAGE+ T P QSFGRCSSLKN+ETRNVNTDYEM
Sbjct: 301 SGFNLGFGGGPPANSTPTRSYNMNHQFKAGEDGTAPTQSFGRCSSLKNEETRNVNTDYEM 360

Query: 361 AYRRHSYNPTDIKFDESDCEEETQLDDGAGGGVDRPPDRNPPPVPSSRVHPKLPDYDTLA 420
           AYRRHSYNPTDIKFDESDCEEET+++D A  GV RPPDRNPPP PSSRVHPKLPDYDTLA
Sbjct: 361 AYRRHSYNPTDIKFDESDCEEETEMEDEADRGVSRPPDRNPPPAPSSRVHPKLPDYDTLA 420

Query: 421 ARFEALKYRK 430
           ARFEALKYRK
Sbjct: 421 ARFEALKYRK 422

BLAST of Spg013484 vs. NCBI nr
Match: XP_022132738.1 (IST1-like protein [Momordica charantia])

HSP 1 Score: 676.0 bits (1743), Expect = 2.1e-190
Identity = 372/431 (86.31%), Postives = 394/431 (91.42%), Query Frame = 0

Query: 2   SAATEAAARSVKIVKKFVSLLRRGFNSSKCKTAAKMAVARIKLLRNKREAVVKQMRRDIA 61
           +AATEAAARS+KIVK F++LLRR FNSSKCKTAAKMAVARIKLLRNKREAVV+QMRRDIA
Sbjct: 49  AAATEAAARSLKIVKLFIALLRRDFNSSKCKTAAKMAVARIKLLRNKREAVVRQMRRDIA 108

Query: 62  LLLQSGQDATARIRVEHVIREQNVLAANEIIELFCELVVARLSIIAKQRECPADLKEGVA 121
           LLLQSGQDATARIRVEHVIREQNVLA NEIIELFCELVVARLSIIAKQRECP DLKEGVA
Sbjct: 109 LLLQSGQDATARIRVEHVIREQNVLAGNEIIELFCELVVARLSIIAKQRECPPDLKEGVA 168

Query: 122 SLIFAAPRCSEIPELSALRNVFEKKYGKDFVSAATDLRPNCGVNRLLIDKLSVRTPSGQV 181
           SLIFAAPRCSEIPELSALRNVFEKKYGKDFVSAATDLRPNCGVNRLLIDKLS+RTPSGQV
Sbjct: 169 SLIFAAPRCSEIPELSALRNVFEKKYGKDFVSAATDLRPNCGVNRLLIDKLSIRTPSGQV 228

Query: 182 KLKIMKEIAKEHQIEWDTTESEKELLKPPEELIEGPRTFVSASSLPVKPAASQPAGDNAQ 241
           KLKIMKEIAKEHQIEWDTTESEKELLKPPEELIEGPRTFVSA+SLPVKP A+Q A D AQ
Sbjct: 229 KLKIMKEIAKEHQIEWDTTESEKELLKPPEELIEGPRTFVSAASLPVKPMANQSARDIAQ 288

Query: 242 IGRQAKEYQMTNSREDESMHFQDTASAAEAAAKAAKQAIAAAQAAAYLANKDSNQDARDS 301
           IGR       TNSRED++ HFQDTASAAEAAAKAAKQAIAAAQAAAYLANKDSN+  R  
Sbjct: 289 IGR------TTNSREDDT-HFQDTASAAEAAAKAAKQAIAAAQAAAYLANKDSNRGGRGG 348

Query: 302 -GFDLGFGVPPANSTPTSSHNMDN-QFKAGEERTTPPQSFGRCSSLK-NDETRNVNTDYE 361
            GF L F   P NS+PT+SHNMDN QFKAGEE T PPQS GRCSSLK N+ETRNVNT+Y+
Sbjct: 349 LGFGLEFEGLPTNSSPTNSHNMDNHQFKAGEETTIPPQSLGRCSSLKNNEETRNVNTNYD 408

Query: 362 MAYRRHSYNPTDIKFDESDCEEETQLDDGAGGGVDRPPDRNPPPVPSSRVHPKLPDYDTL 421
            AYRR+SYNPTDIKFDESDCEEETQ+DD + G  ++PPDRNPPPVPSSRVHPKLPDYDTL
Sbjct: 409 EAYRRYSYNPTDIKFDESDCEEETQMDDESRGATNQPPDRNPPPVPSSRVHPKLPDYDTL 468

Query: 422 AARFEALKYRK 430
           AARFEALKY+K
Sbjct: 469 AARFEALKYKK 472

BLAST of Spg013484 vs. ExPASy Swiss-Prot
Match: Q54I39 (IST1-like protein OS=Dictyostelium discoideum OX=44689 GN=DDB_G0289029 PE=3 SV=1)

HSP 1 Score: 106.3 bits (264), Expect = 8.8e-22
Identity = 110/417 (26.38%), Postives = 188/417 (45.08%), Query Frame = 0

Query: 26  FNSSKCKTAAKMAVARIKLLRNKREAVVKQMRRDIALLLQSGQDATARIRVEHVIREQNV 85
           ++S K K   K+AV+RI++L+NK+  +V+  +R++A LL+   + +ARIRVE +IR++ +
Sbjct: 8   YDSYKLKVQLKLAVSRIQILKNKKANIVRDEKRNVAELLRKKNEESARIRVETIIRDEYL 67

Query: 86  LAANEIIELFCELVVARLSIIAKQRECPADLKEGVASLIFAAPRCSEIPELSALRNVFEK 145
           +   +IIE+ CEL+ AR+++I    E P ++KE + +L++++ R  +IPEL  ++N  + 
Sbjct: 68  IECFQIIEVLCELLHARINLINATTEMPLEMKESIFTLVYSSQRI-QIPELEQIKNQLKA 127

Query: 146 KYGKDFVSAATDLRPNCGVNRLLIDKLSVRTPSGQVKLKIMKEIAKEHQIEW-------- 205
           KYGK   + A +   +  VN  ++ KLS  TP   +  + + EIA++  ++W        
Sbjct: 128 KYGKGLENEA-NCHCSTHVNPKIVHKLSYATPDPSIIFQTLSEIAEKFNVDWCGSDYPPP 187

Query: 206 --------DTTESEKELLKPPEELIEGPRTFVSASSLPVKPAASQPAGDNAQIGRQAKEY 265
                      + + ++L+PP ++I   +          +P   QP     QI +Q ++ 
Sbjct: 188 PQLIMPQPIIVQQQPQILQPPPQIIHHQQ----------QPQILQPP---PQIIQQQQQP 247

Query: 266 QMTNSREDESMHFQDTASAAEAAAKAAKQAIAAAQAAAYLANKDSNQDARDSGFDLGFGV 325
           QM  S    S   Q T S  +   +  +Q         Y   + S Q          F  
Sbjct: 248 QMP-SFPIMSPPQQPTFSQIQHQQQIQQQ---------YQQQQQSPQ----------FPS 307

Query: 326 PPANSTPTSSHNMDNQFKAGEERTTPPQSFGRCSSLKNDETRNVNTDYEMAYRRHSYNPT 385
            P +    +S N   QF        P  S        ND+  N N +Y      ++ N  
Sbjct: 308 APPSFYNNNSGNQTPQF--------PTISTNNSDGYSNDKFNNGNNNYNNNNNNNNNNNN 364

Query: 386 DIKFDESDCEEETQLDDGAGGGVDRPPDRNPPPVPSSRVHPKLPDYDTLAARFEALK 427
           +   + ++                   + N PP          PDYD L ARFEALK
Sbjct: 368 NNNHNNNNNN-----------------NNNIPPPYQPSSDTGYPDYDELTARFEALK 364

BLAST of Spg013484 vs. ExPASy Swiss-Prot
Match: P53990 (IST1 homolog OS=Homo sapiens OX=9606 GN=IST1 PE=1 SV=1)

HSP 1 Score: 105.1 bits (261), Expect = 2.0e-21
Identity = 63/179 (35.20%), Postives = 107/179 (59.78%), Query Frame = 0

Query: 21  LLRRGFNSSKCKTAAKMAVARIKLLRNKREAVVKQMRRDIALLLQSGQDATARIRVEHVI 80
           +L  GF + + +   ++ + R+KLL  K+  + ++ R++IA  L +G+D  ARIRVEH+I
Sbjct: 1   MLGSGFKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHII 60

Query: 81  REQNVLAANEIIELFCELVVARLSIIAKQRECPADLKEGVASLIFAAPRC-SEIPELSAL 140
           RE  ++ A EI+EL+C+L++AR  +I   +E  + L E V++LI+AAPR  SE+ EL  +
Sbjct: 61  REDYLVEAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIV 120

Query: 141 RNVFEKKYGKDFVSAATDLRPNCGVNRLLIDKLSVRTPSGQVKLKIMKEIAKEHQIEWD 199
            +    KY K++       +    VN  L+ KLSV  P   +  + + EIAK + + ++
Sbjct: 121 ADQLCAKYSKEYGKLCRTNQIGT-VNDRLMHKLSVEAPPKILVERYLIEIAKNYNVPYE 178

BLAST of Spg013484 vs. ExPASy Swiss-Prot
Match: Q9CX00 (IST1 homolog OS=Mus musculus OX=10090 GN=Ist1 PE=1 SV=1)

HSP 1 Score: 105.1 bits (261), Expect = 2.0e-21
Identity = 63/179 (35.20%), Postives = 107/179 (59.78%), Query Frame = 0

Query: 21  LLRRGFNSSKCKTAAKMAVARIKLLRNKREAVVKQMRRDIALLLQSGQDATARIRVEHVI 80
           +L  GF + + +   ++ + R+KLL  K+  + ++ R++IA  L +G+D  ARIRVEH+I
Sbjct: 1   MLGSGFKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHII 60

Query: 81  REQNVLAANEIIELFCELVVARLSIIAKQRECPADLKEGVASLIFAAPRC-SEIPELSAL 140
           RE  ++ A EI+EL+C+L++AR  +I   +E  + L E V++LI+AAPR  SE+ EL  +
Sbjct: 61  REDYLVEAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIV 120

Query: 141 RNVFEKKYGKDFVSAATDLRPNCGVNRLLIDKLSVRTPSGQVKLKIMKEIAKEHQIEWD 199
            +    KY K++       +    VN  L+ KLSV  P   +  + + EIAK + + ++
Sbjct: 121 ADQLCAKYSKEYGKLCRTNQIGT-VNDRLMHKLSVEAPPKILVERYLIEIAKNYNVPYE 178

BLAST of Spg013484 vs. ExPASy Swiss-Prot
Match: Q568Z6 (IST1 homolog OS=Rattus norvegicus OX=10116 GN=Ist1 PE=2 SV=1)

HSP 1 Score: 105.1 bits (261), Expect = 2.0e-21
Identity = 63/179 (35.20%), Postives = 107/179 (59.78%), Query Frame = 0

Query: 21  LLRRGFNSSKCKTAAKMAVARIKLLRNKREAVVKQMRRDIALLLQSGQDATARIRVEHVI 80
           +L  GF + + +   ++ + R+KLL  K+  + ++ R++IA  L +G+D  ARIRVEH+I
Sbjct: 1   MLGSGFKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHII 60

Query: 81  REQNVLAANEIIELFCELVVARLSIIAKQRECPADLKEGVASLIFAAPRC-SEIPELSAL 140
           RE  ++ A EI+EL+C+L++AR  +I   +E  + L E V++LI+AAPR  SE+ EL  +
Sbjct: 61  REDYLVEAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIV 120

Query: 141 RNVFEKKYGKDFVSAATDLRPNCGVNRLLIDKLSVRTPSGQVKLKIMKEIAKEHQIEWD 199
            +    KY K++       +    VN  L+ KLSV  P   +  + + EIAK + + ++
Sbjct: 121 ADQLCAKYSKEYGKLCRTNQIGT-VNDRLMHKLSVEAPPKILVERYLIEIAKNYNVPYE 178

BLAST of Spg013484 vs. ExPASy Swiss-Prot
Match: Q5R6G8 (IST1 homolog OS=Pongo abelii OX=9601 GN=IST1 PE=2 SV=1)

HSP 1 Score: 104.0 bits (258), Expect = 4.4e-21
Identity = 63/179 (35.20%), Postives = 106/179 (59.22%), Query Frame = 0

Query: 21  LLRRGFNSSKCKTAAKMAVARIKLLRNKREAVVKQMRRDIALLLQSGQDATARIRVEHVI 80
           +L  GF + + +   ++ + R+KLL  K+  + ++ R++IA  L +G+D  ARIRVEH+I
Sbjct: 1   MLGSGFKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHII 60

Query: 81  REQNVLAANEIIELFCELVVARLSIIAKQRECPADLKEGVASLIFAAPRC-SEIPELSAL 140
           RE  ++ A EI+EL+C+L++AR  +I   +E  + L E V++LI+AAPR  SE+ EL  +
Sbjct: 61  REDYLVEAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIV 120

Query: 141 RNVFEKKYGKDFVSAATDLRPNCGVNRLLIDKLSVRTPSGQVKLKIMKEIAKEHQIEWD 199
            +    KY K +       +    VN  L+ KLSV  P   +  + + EIAK + + ++
Sbjct: 121 ADQLCAKYSKGYGKLCRTNQIGT-VNDRLMHKLSVEAPPKILVERYLIEIAKNYNVPYE 178

BLAST of Spg013484 vs. ExPASy TrEMBL
Match: A0A1S3B2X8 (IST1-like protein OS=Cucumis melo OX=3656 GN=LOC103485176 PE=3 SV=1)

HSP 1 Score: 699.1 bits (1803), Expect = 1.1e-197
Identity = 376/430 (87.44%), Postives = 397/430 (92.33%), Query Frame = 0

Query: 1   MSAATEAAARSVKIVKKFVSLLRRGFNSSKCKTAAKMAVARIKLLRNKREAVVKQMRRDI 60
           MSAATEAAARS+KIVK+F+++LR GFNSSKCKTAAKMAVARIKLLRNKREAVVKQMRRDI
Sbjct: 1   MSAATEAAARSLKIVKQFITILRCGFNSSKCKTAAKMAVARIKLLRNKREAVVKQMRRDI 60

Query: 61  ALLLQSGQDATARIRVEHVIREQNVLAANEIIELFCELVVARLSIIAKQRECPADLKEGV 120
           ALLLQSGQDATARIRVEHVIREQNVLAANE+IELFCELVVARLSIIAKQR+CPADLKEGV
Sbjct: 61  ALLLQSGQDATARIRVEHVIREQNVLAANEVIELFCELVVARLSIIAKQRQCPADLKEGV 120

Query: 121 ASLIFAAPRCSEIPELSALRNVFEKKYGKDFVSAATDLRPNCGVNRLLIDKLSVRTPSGQ 180
           ASLIFAAPRCSEIPELSALRNVFEKKYGKDFVSAA DLRPNCGVNRLLIDKLSVRTP+G+
Sbjct: 121 ASLIFAAPRCSEIPELSALRNVFEKKYGKDFVSAAVDLRPNCGVNRLLIDKLSVRTPTGE 180

Query: 181 VKLKIMKEIAKEHQIEWDTTESEKELLKPPEELIEGPRTFVSASSLPVKPAASQPAGDNA 240
           VKLKIMKEIAKEH+IEWDTTESEKELLKPPEELIEGPRTFVSA+SLPVKP  S    DNA
Sbjct: 181 VKLKIMKEIAKEHKIEWDTTESEKELLKPPEELIEGPRTFVSAASLPVKPIVSH--SDNA 240

Query: 241 QIGRQAKEYQMTNSREDESMHFQDTASAAEAAAKAAKQAIAAAQAAAYLANKDSNQDARD 300
           QI R       T + E+ SMHFQDTASAAEAAAKAAKQAIAAA+AAAYLANKD NQDARD
Sbjct: 241 QIER-------TTNCENGSMHFQDTASAAEAAAKAAKQAIAAAEAAAYLANKDRNQDARD 300

Query: 301 SGFDLGFGVPPANSTPTSSHNMDNQFKAGEERTTPPQSFGRCSSLKNDETRNVNTDYEMA 360
           SGF+LGFG  PANST   S+NMD+QFKAGE+ T P QSFGRC SLKN+ETRNVNTDYEMA
Sbjct: 301 SGFNLGFGGSPANSTSNRSYNMDHQFKAGEDGTAPTQSFGRCFSLKNEETRNVNTDYEMA 360

Query: 361 YRRHSYNPTDIKFDESDCEEETQLDDGAGGGVDRPPDRNPPPVPSSRVHPKLPDYDTLAA 420
           YRRHSYNPTDIKFDESDCEEET++DD AG GV RPPDRNPPP PSSRVHPKLPDYDTLAA
Sbjct: 361 YRRHSYNPTDIKFDESDCEEETEMDDEAGRGVSRPPDRNPPPAPSSRVHPKLPDYDTLAA 420

Query: 421 RFEALKYRKT 431
           RFEALKYRKT
Sbjct: 421 RFEALKYRKT 421

BLAST of Spg013484 vs. ExPASy TrEMBL
Match: A0A5A7SMF5 (IST1-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G00780 PE=3 SV=1)

HSP 1 Score: 699.1 bits (1803), Expect = 1.1e-197
Identity = 376/430 (87.44%), Postives = 397/430 (92.33%), Query Frame = 0

Query: 1   MSAATEAAARSVKIVKKFVSLLRRGFNSSKCKTAAKMAVARIKLLRNKREAVVKQMRRDI 60
           MSAATEAAARS+KIVK+F+++LR GFNSSKCKTAAKMAVARIKLLRNKREAVVKQMRRDI
Sbjct: 1   MSAATEAAARSLKIVKQFITILRCGFNSSKCKTAAKMAVARIKLLRNKREAVVKQMRRDI 60

Query: 61  ALLLQSGQDATARIRVEHVIREQNVLAANEIIELFCELVVARLSIIAKQRECPADLKEGV 120
           ALLLQSGQDATARIRVEHVIREQNVLAANE+IELFCELVVARLSIIAKQR+CPADLKEGV
Sbjct: 61  ALLLQSGQDATARIRVEHVIREQNVLAANEVIELFCELVVARLSIIAKQRQCPADLKEGV 120

Query: 121 ASLIFAAPRCSEIPELSALRNVFEKKYGKDFVSAATDLRPNCGVNRLLIDKLSVRTPSGQ 180
           ASLIFAAPRCSEIPELSALRNVFEKKYGKDFVSAA DLRPNCGVNRLLIDKLSVRTP+G+
Sbjct: 121 ASLIFAAPRCSEIPELSALRNVFEKKYGKDFVSAAVDLRPNCGVNRLLIDKLSVRTPTGE 180

Query: 181 VKLKIMKEIAKEHQIEWDTTESEKELLKPPEELIEGPRTFVSASSLPVKPAASQPAGDNA 240
           VKLKIMKEIAKEH+IEWDTTESEKELLKPPEELIEGPRTFVSA+SLPVKP  S    DNA
Sbjct: 181 VKLKIMKEIAKEHKIEWDTTESEKELLKPPEELIEGPRTFVSAASLPVKPIVSH--SDNA 240

Query: 241 QIGRQAKEYQMTNSREDESMHFQDTASAAEAAAKAAKQAIAAAQAAAYLANKDSNQDARD 300
           QI R       T + E+ SMHFQDTASAAEAAAKAAKQAIAAA+AAAYLANKD NQDARD
Sbjct: 241 QIER-------TTNCENGSMHFQDTASAAEAAAKAAKQAIAAAEAAAYLANKDRNQDARD 300

Query: 301 SGFDLGFGVPPANSTPTSSHNMDNQFKAGEERTTPPQSFGRCSSLKNDETRNVNTDYEMA 360
           SGF+LGFG  PANST   S+NMD+QFKAGE+ T P QSFGRC SLKN+ETRNVNTDYEMA
Sbjct: 301 SGFNLGFGGSPANSTSNRSYNMDHQFKAGEDGTAPTQSFGRCFSLKNEETRNVNTDYEMA 360

Query: 361 YRRHSYNPTDIKFDESDCEEETQLDDGAGGGVDRPPDRNPPPVPSSRVHPKLPDYDTLAA 420
           YRRHSYNPTDIKFDESDCEEET++DD AG GV RPPDRNPPP PSSRVHPKLPDYDTLAA
Sbjct: 361 YRRHSYNPTDIKFDESDCEEETEMDDEAGRGVSRPPDRNPPPAPSSRVHPKLPDYDTLAA 420

Query: 421 RFEALKYRKT 431
           RFEALKYRKT
Sbjct: 421 RFEALKYRKT 421

BLAST of Spg013484 vs. ExPASy TrEMBL
Match: A0A0A0KKW3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G507450 PE=3 SV=1)

HSP 1 Score: 697.6 bits (1799), Expect = 3.3e-197
Identity = 377/430 (87.67%), Postives = 400/430 (93.02%), Query Frame = 0

Query: 1   MSAATEAAARSVKIVKKFVSLLRRGFNSSKCKTAAKMAVARIKLLRNKREAVVKQMRRDI 60
           MSAATEAAARS+KIVK+F+++LR GFNSSKCKTAAKMAVARIKLLRNKREAVVKQMRRDI
Sbjct: 1   MSAATEAAARSLKIVKQFITILRCGFNSSKCKTAAKMAVARIKLLRNKREAVVKQMRRDI 60

Query: 61  ALLLQSGQDATARIRVEHVIREQNVLAANEIIELFCELVVARLSIIAKQRECPADLKEGV 120
           ALLLQSGQDATARIRVEHVIREQNVLAANEIIELFCELVVARLSIIAKQR+CPADLKEGV
Sbjct: 61  ALLLQSGQDATARIRVEHVIREQNVLAANEIIELFCELVVARLSIIAKQRQCPADLKEGV 120

Query: 121 ASLIFAAPRCSEIPELSALRNVFEKKYGKDFVSAATDLRPNCGVNRLLIDKLSVRTPSGQ 180
           ASLIFAAPRCSEIPELSALRNVFEKKYGKDFVSAA DLRPNCGVNRLLIDKLSVRTP+G+
Sbjct: 121 ASLIFAAPRCSEIPELSALRNVFEKKYGKDFVSAAVDLRPNCGVNRLLIDKLSVRTPTGE 180

Query: 181 VKLKIMKEIAKEHQIEWDTTESEKELLKPPEELIEGPRTFVSASSLPVKPAASQPAGDNA 240
           VKLKIMKEIAKEH+IEWDTTESEKELLKP EELIEGPRTFVSA+SLPVKP  S    DNA
Sbjct: 181 VKLKIMKEIAKEHKIEWDTTESEKELLKPSEELIEGPRTFVSAASLPVKPIVSH--SDNA 240

Query: 241 QIGRQAKEYQMTNSREDESMHFQDTASAAEAAAKAAKQAIAAAQAAAYLANKDSNQDARD 300
           QI R       TNSRE+ESMHFQD+ASAAEAAAKAAKQAIAAA+AAAYLANKD N+DA D
Sbjct: 241 QIER------TTNSRENESMHFQDSASAAEAAAKAAKQAIAAAEAAAYLANKDLNRDAGD 300

Query: 301 SGFDLGF-GVPPANSTPTSSHNMDNQFKAGEERTTPPQSFGRCSSLKNDETRNVNTDYEM 360
           SGF+LGF G PPANSTPT S+NM++QFKAGE+ T P QSFGRCSSLKN+ETRNVNTDYEM
Sbjct: 301 SGFNLGFGGGPPANSTPTRSYNMNHQFKAGEDGTAPTQSFGRCSSLKNEETRNVNTDYEM 360

Query: 361 AYRRHSYNPTDIKFDESDCEEETQLDDGAGGGVDRPPDRNPPPVPSSRVHPKLPDYDTLA 420
           AYRRHSYNPTDIKFDESDCEEET+++D A  GV RPPDRNPPP PSSRVHPKLPDYDTLA
Sbjct: 361 AYRRHSYNPTDIKFDESDCEEETEMEDEADRGVSRPPDRNPPPAPSSRVHPKLPDYDTLA 420

Query: 421 ARFEALKYRK 430
           ARFEALKYRK
Sbjct: 421 ARFEALKYRK 422

BLAST of Spg013484 vs. ExPASy TrEMBL
Match: A0A6J1BX61 (IST1-like protein OS=Momordica charantia OX=3673 GN=LOC111005530 PE=3 SV=1)

HSP 1 Score: 676.0 bits (1743), Expect = 1.0e-190
Identity = 372/431 (86.31%), Postives = 394/431 (91.42%), Query Frame = 0

Query: 2   SAATEAAARSVKIVKKFVSLLRRGFNSSKCKTAAKMAVARIKLLRNKREAVVKQMRRDIA 61
           +AATEAAARS+KIVK F++LLRR FNSSKCKTAAKMAVARIKLLRNKREAVV+QMRRDIA
Sbjct: 49  AAATEAAARSLKIVKLFIALLRRDFNSSKCKTAAKMAVARIKLLRNKREAVVRQMRRDIA 108

Query: 62  LLLQSGQDATARIRVEHVIREQNVLAANEIIELFCELVVARLSIIAKQRECPADLKEGVA 121
           LLLQSGQDATARIRVEHVIREQNVLA NEIIELFCELVVARLSIIAKQRECP DLKEGVA
Sbjct: 109 LLLQSGQDATARIRVEHVIREQNVLAGNEIIELFCELVVARLSIIAKQRECPPDLKEGVA 168

Query: 122 SLIFAAPRCSEIPELSALRNVFEKKYGKDFVSAATDLRPNCGVNRLLIDKLSVRTPSGQV 181
           SLIFAAPRCSEIPELSALRNVFEKKYGKDFVSAATDLRPNCGVNRLLIDKLS+RTPSGQV
Sbjct: 169 SLIFAAPRCSEIPELSALRNVFEKKYGKDFVSAATDLRPNCGVNRLLIDKLSIRTPSGQV 228

Query: 182 KLKIMKEIAKEHQIEWDTTESEKELLKPPEELIEGPRTFVSASSLPVKPAASQPAGDNAQ 241
           KLKIMKEIAKEHQIEWDTTESEKELLKPPEELIEGPRTFVSA+SLPVKP A+Q A D AQ
Sbjct: 229 KLKIMKEIAKEHQIEWDTTESEKELLKPPEELIEGPRTFVSAASLPVKPMANQSARDIAQ 288

Query: 242 IGRQAKEYQMTNSREDESMHFQDTASAAEAAAKAAKQAIAAAQAAAYLANKDSNQDARDS 301
           IGR       TNSRED++ HFQDTASAAEAAAKAAKQAIAAAQAAAYLANKDSN+  R  
Sbjct: 289 IGR------TTNSREDDT-HFQDTASAAEAAAKAAKQAIAAAQAAAYLANKDSNRGGRGG 348

Query: 302 -GFDLGFGVPPANSTPTSSHNMDN-QFKAGEERTTPPQSFGRCSSLK-NDETRNVNTDYE 361
            GF L F   P NS+PT+SHNMDN QFKAGEE T PPQS GRCSSLK N+ETRNVNT+Y+
Sbjct: 349 LGFGLEFEGLPTNSSPTNSHNMDNHQFKAGEETTIPPQSLGRCSSLKNNEETRNVNTNYD 408

Query: 362 MAYRRHSYNPTDIKFDESDCEEETQLDDGAGGGVDRPPDRNPPPVPSSRVHPKLPDYDTL 421
            AYRR+SYNPTDIKFDESDCEEETQ+DD + G  ++PPDRNPPPVPSSRVHPKLPDYDTL
Sbjct: 409 EAYRRYSYNPTDIKFDESDCEEETQMDDESRGATNQPPDRNPPPVPSSRVHPKLPDYDTL 468

Query: 422 AARFEALKYRK 430
           AARFEALKY+K
Sbjct: 469 AARFEALKYKK 472

BLAST of Spg013484 vs. ExPASy TrEMBL
Match: A0A6J1GF94 (IST1-like protein OS=Cucurbita moschata OX=3662 GN=LOC111453408 PE=3 SV=1)

HSP 1 Score: 661.8 bits (1706), Expect = 2.0e-186
Identity = 363/426 (85.21%), Postives = 381/426 (89.44%), Query Frame = 0

Query: 7   AAARSVKIVKKFVSLLRRGFNSSKCKTAAKMAVARIKLLRNKREAVVKQMRRDIALLLQS 66
           AA RSV+I+KKF+SLLRRGFNSSKCKTAAKMAVARIKLLRNKREA VKQMRRDIALLLQS
Sbjct: 3   AADRSVRIIKKFISLLRRGFNSSKCKTAAKMAVARIKLLRNKREAAVKQMRRDIALLLQS 62

Query: 67  GQDATARIRVEHVIREQNVLAANEIIELFCELVVARLSIIAKQRECPADLKEGVASLIFA 126
           GQDATARIRVEHVIREQNVLAANEIIELFCELVVARLSIIAKQRECPADLKEGVASLIFA
Sbjct: 63  GQDATARIRVEHVIREQNVLAANEIIELFCELVVARLSIIAKQRECPADLKEGVASLIFA 122

Query: 127 APRCSEIPELSALRNVFEKKYGKDFVSAATDLRPNCGVNRLLIDKLSVRTPSGQVKLKIM 186
           APRCSEIPELSAL NVFEKKYGKDFVSAATDLRPNCGVNRLLIDKLSVRTP+G+VKLK+M
Sbjct: 123 APRCSEIPELSALTNVFEKKYGKDFVSAATDLRPNCGVNRLLIDKLSVRTPAGEVKLKVM 182

Query: 187 KEIAKEHQIEWDTTESEKELLKPPEELIEGPRTFVSASSLPVKP--AASQPAGDNAQIGR 246
           KEIAKEHQIEWDTTESEKELL PPEELI+GPR+FVSA+SLPVKP  AASQ AG++AQI R
Sbjct: 183 KEIAKEHQIEWDTTESEKELLNPPEELIDGPRSFVSAASLPVKPAAAASQSAGNDAQIER 242

Query: 247 QAKEYQMTNSREDESMHFQDTASAAEAAAKAAKQAIAAAQAAAYLANKDSNQDARDSGFD 306
           +       N REDESMHFQDTASAAEAAAKAAKQAIAAA+AAAYLANKD N DARDSGFD
Sbjct: 243 R-------NGREDESMHFQDTASAAEAAAKAAKQAIAAAEAAAYLANKDMNWDARDSGFD 302

Query: 307 LGFGVPPANSTPTSSHNMDNQFKAGEERTTPPQSFGRCSSLKNDETRNVNTDYEMAYRRH 366
           L    PPANSTP  SH++D+QFKAGEERTT                 NVNTDYEMAYRRH
Sbjct: 303 LS---PPANSTPICSHHIDHQFKAGEERTT-----------------NVNTDYEMAYRRH 362

Query: 367 SYNPTDIKFDESDCEEETQLDDGAGGGVDRPPDRNPPPVPSSRVHPKLPDYDTLAARFEA 426
           SYNPTDIKFDESDCEEETQ+DDGA GG  RPPD NPPP+PSSRVHPKLPDYDTLAARFEA
Sbjct: 363 SYNPTDIKFDESDCEEETQMDDGADGGDSRPPDGNPPPIPSSRVHPKLPDYDTLAARFEA 401

Query: 427 LKYRKT 431
           LKYRKT
Sbjct: 423 LKYRKT 401

BLAST of Spg013484 vs. TAIR 10
Match: AT4G35730.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 423.7 bits (1088), Expect = 1.8e-118
Identity = 267/475 (56.21%), Postives = 319/475 (67.16%), Query Frame = 0

Query: 1   MSAATEAAARSVKIVKKFVSLLRRGFNSSKCKTAAKMAVARIKLLRNKREAVVKQMRRDI 60
           M+AA  A+A++ K++K  +SL RRGFNSSKCKTAAKMAVARIKL+RNKR  VVKQMRRDI
Sbjct: 1   MTAAEVASAQTKKLMKFSLSLFRRGFNSSKCKTAAKMAVARIKLIRNKRLVVVKQMRRDI 60

Query: 61  ALLLQSGQDATARIRVEHVIREQNVLAANEIIELFCELVVARLSIIAKQRECPADLKEGV 120
           A+LLQSGQDATARIRVEHVIREQN+ AANEIIELFCEL+V+RL+II KQ++CP DLKEG+
Sbjct: 61  AVLLQSGQDATARIRVEHVIREQNIQAANEIIELFCELIVSRLTIITKQKQCPVDLKEGI 120

Query: 121 ASLIFAAPRCSEIPELSALRNVFEKKYGKDFVSAATDLRPNCGVNRLLIDKLSVRTPSGQ 180
           ASLIFAAPRCSEIPEL  LR++F KKYGKDFVSAATDLRP+CGVNR+LIDKLSVR P G+
Sbjct: 121 ASLIFAAPRCSEIPELGDLRDIFAKKYGKDFVSAATDLRPSCGVNRMLIDKLSVRNPGGE 180

Query: 181 VKLKIMKEIAKEHQIEWDTTESEKELLKPPEELIEGPRTFVSASSLPVKPAA-SQPAGDN 240
            KLKIMKEIAKE Q++WDTTE+E+ELLKP EE I+GPR FVSASSLPV  AA ++P    
Sbjct: 181 YKLKIMKEIAKEFQVDWDTTETEQELLKPQEESIDGPRKFVSASSLPVNRAAINEPIDPT 240

Query: 241 AQIGRQAKEYQMTNSREDESMHFQDTASAAEAAAKAAKQAIAAAQAAAYLANK------- 300
             + R      +       + H+ DT SAAEAA + AKQA+AAAQ A+ LA +       
Sbjct: 241 KAVPRSTSSMSI-------NTHYHDTESAAEAATELAKQAVAAAQVASLLATRRDSSNKE 300

Query: 301 -----DSNQDARDSGF-DLGFGVPPANSTPTSSHNMDNQFKAGEERTTPPQSFGRCSSLK 360
                D +   +DS + D     P +      S       K G E     +  GR  S  
Sbjct: 301 FSVSSDHSTHQKDSQYMDHHHHHPGSRRQSRDSETSSYYAKPGAEN----RGMGRRHSYN 360

Query: 361 NDETRNVNTDYEMAY------------RRHSYNP--------TDIKFDESD-CEEETQLD 420
           N       +DYE  Y            RRHSYNP        ++IKFDESD  EEET+ D
Sbjct: 361 NPGIN--ESDYEEEYTNTEAEAKETMRRRHSYNPRSVPPPATSEIKFDESDYYEEETEPD 420

Query: 421 DGAGGGVDR-PPDRNPPPVPSS----------RVHPKLPDYDTLAARFEALKYRK 430
           + + G V   PP+R PP  P S          +VHPKLPDYD LAARFEA+++ K
Sbjct: 421 EPSQGRVSSLPPNRAPPQAPQSGESRQDSSGHQVHPKLPDYDILAARFEAIRHSK 462

BLAST of Spg013484 vs. TAIR 10
Match: AT1G34220.2 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 238.4 bits (607), Expect = 1.1e-62
Identity = 128/266 (48.12%), Postives = 185/266 (69.55%), Query Frame = 0

Query: 20  SLLRRGFNSSKCKTAAKMAVARIKLLRNKREAVVKQMRRDIALLLQSGQDATARIRVEHV 79
           S   +GF ++KCKT  K+ + RIKL+RN+REA +KQMRR+IA LL++GQ+ATARIRVEH+
Sbjct: 6   SFFNKGFKAAKCKTLLKLTIPRIKLIRNRREAQIKQMRREIAKLLETGQEATARIRVEHI 65

Query: 80  IREQNVLAANEIIELFCELVVARLSIIAKQRECPADLKEGVASLIFAAPRCSEIPELSAL 139
           IRE+ ++AA EI+ELFCEL+  RL II  QRECP DLKE ++S+ FAAPRCS++ EL  +
Sbjct: 66  IREEKMMAAQEILELFCELIAVRLPIIEAQRECPLDLKEAISSVCFAAPRCSDLTELQQV 125

Query: 140 RNVFEKKYGKDFVSAATDLRPNCGVNRLLIDKLSVRTPSGQVKLKIMKEIAKEHQIEWDT 199
           + +F  KYGK+FV+AA++L+P+ GVNR L++ LSVR PS + KLK++KEIA+EH+++WD 
Sbjct: 126 QILFVSKYGKEFVAAASELKPDSGVNRKLVELLSVRAPSPETKLKLLKEIAEEHELDWDP 185

Query: 200 TESEKELLKPPEELIEGPRTFVSASSLPVKPAASQPAGDNAQIGRQAK-----EYQMTNS 259
             +E +L K  E+L++GP+ F   S LP+    ++     +    + K     EY + + 
Sbjct: 186 ASTETDLFKSHEDLLDGPKQFGGGSKLPLPEEQNEKTNLTSLSAAKEKSDSDSEYDILDF 245

Query: 260 REDESMHFQDTASAAEA-AAKAAKQA 280
            E  ++  + T  A    A  AAK A
Sbjct: 246 PEVPNVLLRPTPGATSVNAPDAAKSA 271

BLAST of Spg013484 vs. TAIR 10
Match: AT1G34220.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 222.6 bits (566), Expect = 6.0e-58
Identity = 128/296 (43.24%), Postives = 185/296 (62.50%), Query Frame = 0

Query: 20  SLLRRGFNSSKCKTAAKMAVARIKLLRNKREAVVKQMRRDIALLLQSGQDATARIRVEHV 79
           S   +GF ++KCKT  K+ + RIKL+RN+REA +KQMRR+IA LL++GQ+ATARIRVEH+
Sbjct: 6   SFFNKGFKAAKCKTLLKLTIPRIKLIRNRREAQIKQMRREIAKLLETGQEATARIRVEHI 65

Query: 80  IREQNVLAANEIIELFCELVVARLSIIAKQRECPADLKEGVASLIFAAPRCSEIPELSAL 139
           IRE+ ++AA EI+ELFCEL+  RL II  QRECP DLKE ++S+ FAAPRCS++ EL  +
Sbjct: 66  IREEKMMAAQEILELFCELIAVRLPIIEAQRECPLDLKEAISSVCFAAPRCSDLTELQQV 125

Query: 140 RNVFEKKYGKDFVSAATDLRPNCGVNR------------------------------LLI 199
           + +F  KYGK+FV+AA++L+P+ GVNR                               L+
Sbjct: 126 QILFVSKYGKEFVAAASELKPDSGVNRKTESLIFIAWFSLVETRDLFMFLYFSNSILQLV 185

Query: 200 DKLSVRTPSGQVKLKIMKEIAKEHQIEWDTTESEKELLKPPEELIEGPRTFVSASSLPVK 259
           + LSVR PS + KLK++KEIA+EH+++WD   +E +L K  E+L++GP+ F   S LP+ 
Sbjct: 186 ELLSVRAPSPETKLKLLKEIAEEHELDWDPASTETDLFKSHEDLLDGPKQFGGGSKLPLP 245

Query: 260 PAASQPAGDNAQIGRQAK-----EYQMTNSREDESMHFQDTASAAEA-AAKAAKQA 280
              ++     +    + K     EY + +  E  ++  + T  A    A  AAK A
Sbjct: 246 EEQNEKTNLTSLSAAKEKSDSDSEYDILDFPEVPNVLLRPTPGATSVNAPDAAKSA 301

BLAST of Spg013484 vs. TAIR 10
Match: AT1G25420.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 213.8 bits (543), Expect = 2.8e-55
Identity = 123/297 (41.41%), Postives = 187/297 (62.96%), Query Frame = 0

Query: 21  LLRRGFNSSKCKTAAKMAVARIKLLRNKREAVVKQMRRDIALLLQSGQDATARIRVEHVI 80
           L  RG   +KCKT+  +A+AR+KLL+NKR+  +K M+++IA  LQ+GQ+  ARIRVEHVI
Sbjct: 7   LFNRGIFGAKCKTSLNLAIARMKLLQNKRDMQLKHMKKEIAHFLQAGQEPIARIRVEHVI 66

Query: 81  REQNVLAANEIIELFCELVVARLSIIAKQRECPADLKEGVASLIFAAPRCSEIPELSALR 140
           RE N+ AA EI+ELFCE ++AR+ I+  ++ECP +L+E +AS+IFAAPRCSE+P+L  ++
Sbjct: 67  REMNLWAAYEILELFCEFILARVPILESEKECPRELREAIASIIFAAPRCSEVPDLLQIK 126

Query: 141 NVFEKKYGKDFVSAATDLRPNCGVNRLLIDKLSVRTPSGQVKLKIMKEIAKEHQIEWDTT 200
           N+F  KYGK+F+  A++LRP+ GVNR +I+KLS  +PSG  +LK++KEIA+E+ + WD++
Sbjct: 127 NLFGTKYGKEFIMVASELRPDSGVNRTIIEKLSPTSPSGAARLKMLKEIAQEYSLNWDSS 186

Query: 201 ESEKELLKPPEELIEGPRTFVSASSLPVKPAASQPAGDN----------AQIGRQAKEYQ 260
            +E E +K  E+L+ G +       +     + Q  G +          A+  ++ ++ Q
Sbjct: 187 ATEAEFMKSHEDLLGGAKQIHRQDGISESRPSQQGYGQSSVSREVESLPAEATQRFQKLQ 246

Query: 261 MTN----------------SREDESMHFQDTASAAEAAAKAAKQAIAAAQAAAYLAN 292
             N                +  D   +  D    A AA  +A +A AAA+AAA L N
Sbjct: 247 AQNPVSKSMPSSKLTSAFQAPPDTRRNQTDVMEIARAALASADRATAAARAAAQLVN 303

BLAST of Spg013484 vs. TAIR 10
Match: AT2G19710.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 203.0 bits (515), Expect = 4.9e-52
Identity = 134/346 (38.73%), Postives = 196/346 (56.65%), Query Frame = 0

Query: 21  LLRRGFNSSKCKTAAKMAVARIKLLRNKREAVVKQMRRDIALLLQSGQDATARIRVEHVI 80
           +L+RGF  +KCKTA +MA +R+K+L+NK+E  +KQ+RR++A LL+SGQ  TARIRVEHV+
Sbjct: 4   VLQRGFKPAKCKTALQMANSRLKILKNKKEIQIKQLRRELAQLLESGQTPTARIRVEHVV 63

Query: 81  REQNVLAANEIIELFCELVVARLSIIAKQRECPADLKEGVASLIFAAPRCSEIPELSALR 140
           RE+  +AA E+I ++CEL+V RL +I  Q+ CP DLKE V S++FA+ R S++PELS + 
Sbjct: 64  REEKTVAAYELIGIYCELLVVRLGVIESQKNCPIDLKEAVTSVLFASQRLSDVPELSEIF 123

Query: 141 NVFEKKYGKDFVSAATDLRPNCGVNRLLIDKLSVRTPSGQVKLKIMKEIAKEHQIEWDTT 200
             F  KYGKDF ++A +LRP+ GV+RLL++KLS + P G  K+KI+  IA+EH + W+  
Sbjct: 124 KQFTTKYGKDFSTSAVELRPDSGVSRLLVEKLSAKAPDGPTKVKILMAIAEEHNVVWE-A 183

Query: 201 ESEKELLKPPEELIEGPRTFVSASSLPV----------KPAASQPAGDNAQIGRQAKEY- 260
           +S  E      EL+ G  +F  ASS+ +           P    PA  NA  G   + + 
Sbjct: 184 QSFVESDPKDTELLNGANSFQPASSMNMDSSINSNKEQPPNIHAPATVNAHHGSSERHHS 243

Query: 261 ----------------QMTNSREDESMH-------------------------------- 302
                            +T+ + D+  H                                
Sbjct: 244 PENSYANGGRSSSRSNNVTSGKADDYYHSKARPSRSRPDEGECRNPNHGYENSSSRNKQK 303