Spg013144 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg013144 vs. NCBI nr
Match: XP_022144069.1 (MACPF domain-containing protein CAD1-like [Momordica charantia])

HSP 1 Score: 1102.8 bits (2851), Expect = 0.0e+00
Identity = 541/586 (92.32%), Postives = 559/586 (95.39%), Query Frame = 0

Query: 1   MGESGTALHTAINAVQALGRGFDVNFDTRLLYCKGVAGSTVVEVDDKHTRDLRLYDDVVL 60
           MG++GTALHTAINAVQALGRGFDVNFDTRLLYCKGV+GST+VEVDD+HTRD+ LYDDVVL
Sbjct: 1   MGDTGTALHTAINAVQALGRGFDVNFDTRLLYCKGVSGSTIVEVDDEHTRDICLYDDVVL 60

Query: 61  LNVSRDIKNFQDQEPVGHRSSGICSFDEMVDYFNQRDGLSEGYSSGSFNSAFGFTGSKQT 120
           LNVS DIKNF  QEP+GHRSSGICSFDEMV+YFNQ+DGLSEGY+SGSFNSAFGF GSKQ 
Sbjct: 61  LNVSWDIKNF--QEPIGHRSSGICSFDEMVNYFNQKDGLSEGYTSGSFNSAFGFNGSKQI 120

Query: 121 DAVATKSLSTDGFYIPLAKFQLTNTPFVLQENVKRAVPTSWDPPSLASFIENFGTHVITS 180
           DA  TK+LS DGFYIPLAKFQ T TP VLQENVKRA+PTSWDPPSLASFIENFGTHVITS
Sbjct: 121 DAATTKNLSMDGFYIPLAKFQFTKTPLVLQENVKRAIPTSWDPPSLASFIENFGTHVITS 180

Query: 181 ITIGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSGTQSQTGSGSIKLKEKGLDGGLF 240
           ITIGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFS TQ  TGSGSIKLKEKGLDGGLF
Sbjct: 181 ITIGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGLTGSGSIKLKEKGLDGGLF 240

Query: 241 NNQGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFYPITA 300
           NNQGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVR SPDVIEMTF+PITA
Sbjct: 241 NNQGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRLSPDVIEMTFFPITA 300

Query: 301 LLDGVAGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPVRDTIPGHQRKEPVCPSL 360
           LLDGV GKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPVRDT+PGHQRKEPVCPSL
Sbjct: 301 LLDGVTGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPVRDTVPGHQRKEPVCPSL 360

Query: 361 QFSIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKFLQPYWDTHV 420
           QFSIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPK LQPYWDTHV
Sbjct: 361 QFSIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKILQPYWDTHV 420

Query: 421 AIGAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDF 480
            IGAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDF
Sbjct: 421 GIGAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDF 480

Query: 481 GSRNVLYMKLLYSRLPGCTIRRTLWDHLPNDKSQKLVSSSVNSNNADDSSSGSNSIQGNK 540
           G RNVLYMKLLYSRLPGCTI+RTLWDHL NDKS+KLVSSS NS NADDSSSGS +IQGNK
Sbjct: 481 GPRNVLYMKLLYSRLPGCTIQRTLWDHLLNDKSKKLVSSSDNS-NADDSSSGSRNIQGNK 540

Query: 541 LAKLVDMTEMCKGPQDPPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 587
           LAKLVD +EM KGP DPPGHWLVTGAKLGVEKGKIVLRVKYSLLNY
Sbjct: 541 LAKLVDTSEMSKGPHDPPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 583

BLAST of Spg013144 vs. NCBI nr
Match: XP_038882143.1 (MACPF domain-containing protein CAD1-like [Benincasa hispida])

HSP 1 Score: 1096.6 bits (2835), Expect = 0.0e+00
Identity = 535/586 (91.30%), Postives = 557/586 (95.05%), Query Frame = 0

Query: 1   MGESGTALHTAINAVQALGRGFDVNFDTRLLYCKGVAGSTVVEVDDKHTRDLRLYDDVVL 60
           MGESGTALHTA+NAVQALGRGFDVNFD+RLLYCKGV+GST+VE+DD+H+RD+ LYDDVVL
Sbjct: 1   MGESGTALHTAMNAVQALGRGFDVNFDSRLLYCKGVSGSTIVEIDDEHSRDICLYDDVVL 60

Query: 61  LNVSRDIKNFQDQEPVGHRSSGICSFDEMVDYFNQRDGLSEGYSSGSFNSAFGFTGSKQT 120
           LNVS DIKNF  QEP+GHRSSGICSFDEMVDYFNQ+DGLSEGY SGSFNSAFGFTG KQ 
Sbjct: 61  LNVSWDIKNF--QEPIGHRSSGICSFDEMVDYFNQKDGLSEGYPSGSFNSAFGFTGFKQI 120

Query: 121 DAVATKSLSTDGFYIPLAKFQLTNTPFVLQENVKRAVPTSWDPPSLASFIENFGTHVITS 180
           DA ATKSLSTDGFYIPLAKFQ T TP VLQENVKRAVPT+WDPPSLASFIENFGTHVITS
Sbjct: 121 DAAATKSLSTDGFYIPLAKFQFTKTPLVLQENVKRAVPTTWDPPSLASFIENFGTHVITS 180

Query: 181 ITIGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSGTQSQTGSGSIKLKEKGLDGGLF 240
           ITIGGKDVIY+KQHQSSPLSTME+KRYVQDIANQRFS TQ Q GSGSIK KEKGLDGGLF
Sbjct: 181 ITIGGKDVIYIKQHQSSPLSTMELKRYVQDIANQRFSDTQGQMGSGSIKHKEKGLDGGLF 240

Query: 241 NNQGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFYPITA 300
           NNQGVYPQPTSAPYLTGKEDVTVIFRRRGG+DLEQNHVRWAR VRSSPDVIEMTF PITA
Sbjct: 241 NNQGVYPQPTSAPYLTGKEDVTVIFRRRGGNDLEQNHVRWARNVRSSPDVIEMTFCPITA 300

Query: 301 LLDGVAGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPVRDTIPGHQRKEPVCPSL 360
           LLDGVAGKEHLTRAILLYL+YKPPLEELRYFLEFQIPRVWAP  DTIPGHQRKEPVCPSL
Sbjct: 301 LLDGVAGKEHLTRAILLYLDYKPPLEELRYFLEFQIPRVWAPTCDTIPGHQRKEPVCPSL 360

Query: 361 QFSIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKFLQPYWDTHV 420
           QFSIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPK + PYWDTHV
Sbjct: 361 QFSIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMHPYWDTHV 420

Query: 421 AIGAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDF 480
           AIGAPKWQGPEEQDSRWFEPVKWKNFSH+STAPIENPETFMGELSGVYIVTGAQLGVWDF
Sbjct: 421 AIGAPKWQGPEEQDSRWFEPVKWKNFSHISTAPIENPETFMGELSGVYIVTGAQLGVWDF 480

Query: 481 GSRNVLYMKLLYSRLPGCTIRRTLWDHLPNDKSQKLVSSSVNSNNADDSSSGSNSIQGNK 540
           GSRNVLYMKLLYSRLPGCTIRR LWDHLPNDKS+KLV+SS NS+  DDSSSG+ SIQGNK
Sbjct: 481 GSRNVLYMKLLYSRLPGCTIRRMLWDHLPNDKSKKLVTSSDNSSKVDDSSSGTKSIQGNK 540

Query: 541 LAKLVDMTEMCKGPQDPPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 587
           LAK+VD TEM KGPQD PGHWLVTGAKLGVEKGKIVLRVKYSLLNY
Sbjct: 541 LAKVVDTTEMSKGPQDSPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 584

BLAST of Spg013144 vs. NCBI nr
Match: XP_008463260.1 (PREDICTED: MACPF domain-containing protein CAD1-like [Cucumis melo] >TYK09776.1 MACPF domain-containing protein CAD1-like [Cucumis melo var. makuwa])

HSP 1 Score: 1092.0 bits (2823), Expect = 0.0e+00
Identity = 539/588 (91.67%), Postives = 557/588 (94.73%), Query Frame = 0

Query: 1   MGESGTALHTAINAVQALGRGFDVNFDTRLLYCKGVAGSTVVEVDDKHTRDLRLYDDVVL 60
           MGESGTALHTA NAVQALGRGFDVNFDTRLLYCKGVAGST+VEVDD+H+RD+ LYDDVVL
Sbjct: 1   MGESGTALHTASNAVQALGRGFDVNFDTRLLYCKGVAGSTIVEVDDEHSRDICLYDDVVL 60

Query: 61  LNVSRDIKNFQDQEPVGHRSSGICSFDEMVDYFNQRDGLSEGYSSGSFNSAFGFTGSKQT 120
            NVS DIKNF  QEP+GHRSSGICSFDEM DYFNQ+DGLSEGY SGSFNSAFGFTG +QT
Sbjct: 61  HNVSWDIKNF--QEPIGHRSSGICSFDEMADYFNQKDGLSEGYPSGSFNSAFGFTGLRQT 120

Query: 121 DAVATKSLSTDGFYIPLAKFQLTNTPFVLQENVKRAVPTSWDPPSLASFIENFGTHVITS 180
           DA ATKSLSTDGFYIPLAKFQ T TP  L+EN+KRAVPT+WDPPSLASFIENFGTHVITS
Sbjct: 121 DASATKSLSTDGFYIPLAKFQFTKTPLELKENIKRAVPTTWDPPSLASFIENFGTHVITS 180

Query: 181 ITIGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSGTQSQTGSGSIKLKEKGLDGGLF 240
           ITIGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFS TQ QTGSGSIKLKEKGLDGGLF
Sbjct: 181 ITIGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGLDGGLF 240

Query: 241 NNQGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFYPITA 300
           NNQGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFYPITA
Sbjct: 241 NNQGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFYPITA 300

Query: 301 LLDGVAGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPVRDTIPGHQRKEPVCPSL 360
           LLDGV GKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAP+ D +PG QRKEPVCPSL
Sbjct: 301 LLDGVVGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPIHDKLPGRQRKEPVCPSL 360

Query: 361 QFSIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKFLQPYWDTHV 420
           QFSIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPK + PYWDTHV
Sbjct: 361 QFSIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMHPYWDTHV 420

Query: 421 AIGAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDF 480
           AIGAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDF
Sbjct: 421 AIGAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDF 480

Query: 481 GSRNVLYMKLLYSRLPGCTIRRTLWDHLPN-DKSQKLVSSSVNSNNADDSSSGSNSIQG- 540
           GSRNVLYMKLLYSRLPGCTIRRTLWDHLP  +KS+KLVSSS N+NNAD+SSSG+ S QG 
Sbjct: 481 GSRNVLYMKLLYSRLPGCTIRRTLWDHLPPIEKSKKLVSSSDNTNNADNSSSGTKSTQGN 540

Query: 541 NKLAKLVDMTEMCKGPQDPPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 587
           NKLAKLVD TEM KGPQD PGHWLVTGAKLGVEKGKIVLRVKYSLLNY
Sbjct: 541 NKLAKLVDTTEMSKGPQDSPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 586

BLAST of Spg013144 vs. NCBI nr
Match: XP_022925277.1 (MACPF domain-containing protein CAD1-like [Cucurbita moschata])

HSP 1 Score: 1088.2 bits (2813), Expect = 0.0e+00
Identity = 536/587 (91.31%), Postives = 556/587 (94.72%), Query Frame = 0

Query: 1   MGESGTALHTAINAVQALGRGFDVNFDTRLLYCKGVAGSTVVEVDDKHTRDLRLYDDVVL 60
           M ESGTALHTAINAVQALGRGFDVNFDTRLLYCKGVAGST+VEVDD+HTRD+ LYDDVVL
Sbjct: 1   MEESGTALHTAINAVQALGRGFDVNFDTRLLYCKGVAGSTIVEVDDEHTRDICLYDDVVL 60

Query: 61  LNVSRDIKNFQDQEPVGHRSSGICSFDEMVDYFNQRDGLSEGYSSGSFNSAFGFTGSKQT 120
           LNVS DIKNF  Q+P+GHRSSGICSFDEMVDYFNQ+DGLSEGY SGSFN+AFGFTGSKQ 
Sbjct: 61  LNVSWDIKNF--QQPIGHRSSGICSFDEMVDYFNQKDGLSEGYPSGSFNTAFGFTGSKQI 120

Query: 121 DAVATKSLSTDGFYIPLAKFQLTNTPFVLQENVKRAVPTSWDPPSLASFIENFGTHVITS 180
           DA A K+LSTDGFYIPLAKFQLT+T  +L ENVKR+VPTSWDPP+LASFIENFGTHVITS
Sbjct: 121 DAAAAKNLSTDGFYIPLAKFQLTSTSLLLHENVKRSVPTSWDPPALASFIENFGTHVITS 180

Query: 181 ITIGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSGTQSQTGSGSIKLKEKGLDGGLF 240
           ITIGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFS TQ Q GSGS KLKEKGLDGGLF
Sbjct: 181 ITIGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQAGSGSRKLKEKGLDGGLF 240

Query: 241 NNQGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFYPITA 300
           NNQGVYPQPTSAPY +GKEDVTVIFRRRGGDDLEQNHV+WA+TVRSSPDVIEMTFYPITA
Sbjct: 241 NNQGVYPQPTSAPYPSGKEDVTVIFRRRGGDDLEQNHVKWAKTVRSSPDVIEMTFYPITA 300

Query: 301 LLDGVAGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPVRDTIPGHQRKEPVCPSL 360
           LLDGVAGKEHLTRAI LYLEYKPPLEELRYFLEFQIPRVWAPVRDTIPGHQRKEPVCPSL
Sbjct: 301 LLDGVAGKEHLTRAIRLYLEYKPPLEELRYFLEFQIPRVWAPVRDTIPGHQRKEPVCPSL 360

Query: 361 QFSIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKFLQPYWDTHV 420
           QFSIMGQKLYVSIEQI IGRKPVTGM LSLEG KQNRLSIHLQHLA+LPK LQPYWDTH+
Sbjct: 361 QFSIMGQKLYVSIEQILIGRKPVTGMCLSLEGGKQNRLSIHLQHLATLPKILQPYWDTHI 420

Query: 421 AIGAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDF 480
           AIGAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVW F
Sbjct: 421 AIGAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWGF 480

Query: 481 GSRNVLYMKLLYSRLPGCTIRRTLWDHLPNDKSQKLVSSSVNSN-NADDSSSGSNSIQGN 540
           GSRNVLYMKLLYSRLPGCTIRRTLWDHLPNDKS+KLVSSS NSN  ADDSSSGS +IQGN
Sbjct: 481 GSRNVLYMKLLYSRLPGCTIRRTLWDHLPNDKSKKLVSSSDNSNAAADDSSSGSKNIQGN 540

Query: 541 KLAKLVDMTEMCKGPQDPPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 587
           KLAKLVD TEM KGPQD PGHWLVTGAKLGVEK +IVLRVKYSLLNY
Sbjct: 541 KLAKLVDTTEMSKGPQDSPGHWLVTGAKLGVEKSRIVLRVKYSLLNY 585

BLAST of Spg013144 vs. NCBI nr
Match: XP_023517935.1 (MACPF domain-containing protein CAD1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1085.9 bits (2807), Expect = 0.0e+00
Identity = 535/587 (91.14%), Postives = 555/587 (94.55%), Query Frame = 0

Query: 1   MGESGTALHTAINAVQALGRGFDVNFDTRLLYCKGVAGSTVVEVDDKHTRDLRLYDDVVL 60
           M ESGTALHTAINAVQALGRGFDVNFDTRLLYCKGVAGST+VEVDD+HTRD+ LYDDVVL
Sbjct: 1   MEESGTALHTAINAVQALGRGFDVNFDTRLLYCKGVAGSTIVEVDDEHTRDICLYDDVVL 60

Query: 61  LNVSRDIKNFQDQEPVGHRSSGICSFDEMVDYFNQRDGLSEGYSSGSFNSAFGFTGSKQT 120
           LNVS DIKNF  Q+P+GHRSSGICSFDEMVDYFNQ+DGLSEGY SGSFN+AFGFTGSKQ 
Sbjct: 61  LNVSWDIKNF--QQPIGHRSSGICSFDEMVDYFNQKDGLSEGYPSGSFNTAFGFTGSKQI 120

Query: 121 DAVATKSLSTDGFYIPLAKFQLTNTPFVLQENVKRAVPTSWDPPSLASFIENFGTHVITS 180
           DA A K+LSTDGFYIPLAKFQLT+T  +L ENVKR+VPTSWDPP+LASFIENFGTHVITS
Sbjct: 121 DAAAAKNLSTDGFYIPLAKFQLTSTSLLLHENVKRSVPTSWDPPALASFIENFGTHVITS 180

Query: 181 ITIGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSGTQSQTGSGSIKLKEKGLDGGLF 240
           ITIGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFS TQ Q GSGS KLKEKGLDGGLF
Sbjct: 181 ITIGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQAGSGSRKLKEKGLDGGLF 240

Query: 241 NNQGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFYPITA 300
           NNQGVYPQPTSAPY +GKEDVTVIFRRRGGDDLEQNHV+WA+TVRSSPDVIEMTFYPITA
Sbjct: 241 NNQGVYPQPTSAPYPSGKEDVTVIFRRRGGDDLEQNHVKWAKTVRSSPDVIEMTFYPITA 300

Query: 301 LLDGVAGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPVRDTIPGHQRKEPVCPSL 360
           LLDGVAGKEHLTRAI LYLEYKPPLEELRYFLEFQIPRVWAPVRDTIPGHQRKEPVCPSL
Sbjct: 301 LLDGVAGKEHLTRAIRLYLEYKPPLEELRYFLEFQIPRVWAPVRDTIPGHQRKEPVCPSL 360

Query: 361 QFSIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKFLQPYWDTHV 420
           QFSIMGQKLYVSIEQI IGRKPVTGM LSLEG KQNRLSIHLQHLA+LPK LQPYWDTH+
Sbjct: 361 QFSIMGQKLYVSIEQILIGRKPVTGMCLSLEGGKQNRLSIHLQHLATLPKILQPYWDTHI 420

Query: 421 AIGAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDF 480
           AIGAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVW F
Sbjct: 421 AIGAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWGF 480

Query: 481 GSRNVLYMKLLYSRLPGCTIRRTLWDHLPNDKSQKLVSSSVNSNN-ADDSSSGSNSIQGN 540
           GSR VLYMKLLYSRLPGCTIRRTLWDHLPNDKS+KLVSSS NSN  ADDSSSGS +IQGN
Sbjct: 481 GSRYVLYMKLLYSRLPGCTIRRTLWDHLPNDKSKKLVSSSDNSNTAADDSSSGSKNIQGN 540

Query: 541 KLAKLVDMTEMCKGPQDPPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 587
           KLAKLVD TEM KGPQD PGHWLVTGAKLGVEK +IVLRVKYSLLNY
Sbjct: 541 KLAKLVDTTEMSKGPQDSPGHWLVTGAKLGVEKSRIVLRVKYSLLNY 585

BLAST of Spg013144 vs. ExPASy Swiss-Prot
Match: Q9C7N2 (MACPF domain-containing protein CAD1 OS=Arabidopsis thaliana OX=3702 GN=CAD1 PE=2 SV=1)

HSP 1 Score: 701.4 bits (1809), Expect = 8.4e-201
Identity = 345/584 (59.08%), Postives = 430/584 (73.63%), Query Frame = 0

Query: 4   SGTALHTAI-NAVQALGRGFDVNFDTRLLYCKGVAGSTVVEVDDKHTRDLRLYDDVVLLN 63
           S  AL T + NA+QALGRGFDV  D RLLYCKG  GS +V +++   RDL L    +L N
Sbjct: 13  SSEALTTTLRNAIQALGRGFDVTSDVRLLYCKGAPGSRLVRIEEGQNRDLELSHGFLLPN 72

Query: 64  VSRDIKNFQDQEPVGHRSSGICSFDEMVDYFNQRDGLSEGYSSGSFNSAFGFTGSKQTDA 123
           V  DI   +     G +   +CSF EM + FN R G+      G FN+ F +TGS Q DA
Sbjct: 73  VPADIDCSRGNS--GTQRISVCSFHEMAEEFNVRSGVKGNIPLGCFNAMFNYTGSWQVDA 132

Query: 124 VATKSLSTDGFYIPLAKFQLTNTPFVLQENVKRAVPTSWDPPSLASFIENFGTHVITSIT 183
            +TKSL+  G++IPL   +L     VL   ++RAVP+SWDP SLASFIEN+GTH++TS+T
Sbjct: 133 ASTKSLALVGYFIPLYDVKLAKLTLVLHNEIRRAVPSSWDPASLASFIENYGTHIVTSVT 192

Query: 184 IGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSGTQSQTGSGSIKLKEKGLDGGLFNN 243
           IGG+DV+Y++QHQSSPL   E++ YV D+   RF   +SQ+ +G +K K+K         
Sbjct: 193 IGGRDVVYIRQHQSSPLPVSEIENYVNDMIKHRFHEAESQSITGPLKYKDK--------- 252

Query: 244 QGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFYPITALL 303
                            D+TVIFRRRGGDDLEQ+H RWA TV ++PD+I MTF PI +LL
Sbjct: 253 -----------------DITVIFRRRGGDDLEQSHARWAETVPAAPDIINMTFTPIVSLL 312

Query: 304 DGVAGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPVRDTIPGHQRKEPVCPSLQF 363
           +GV G  HLTRAI LYLEYKPP+E+L+YFL++QI R WAP +  +   QRKEPVC SLQF
Sbjct: 313 EGVPGLRHLTRAIELYLEYKPPIEDLQYFLDYQIARAWAPEQSNL---QRKEPVCSSLQF 372

Query: 364 SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKFLQPYWDTHVAI 423
           S+MG KL++S +Q+++GRKPVTG+RLSLEGSKQNRLSIHLQHL SLPK LQP+WD+HV I
Sbjct: 373 SLMGPKLFISADQVTVGRKPVTGLRLSLEGSKQNRLSIHLQHLVSLPKILQPHWDSHVPI 432

Query: 424 GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFGS 483
           GAPKWQGPEEQDSRWFEP+KWKNFSHVST+PIE+ ET +G+LSGV+IVTGAQLGVWDFGS
Sbjct: 433 GAPKWQGPEEQDSRWFEPIKWKNFSHVSTSPIEHTETHIGDLSGVHIVTGAQLGVWDFGS 492

Query: 484 RNVLYMKLLYSRLPGCTIRRTLWDHLPNDKSQKLVSSSVNSNNADDSSSGSNSIQGNKLA 543
           +NVL++KLL+S++PGCTIRR++WDH P   S +L     +++++ +  SG    Q  KLA
Sbjct: 493 KNVLHLKLLFSKVPGCTIRRSVWDHTPVASSGRLEPGGPSTSSSTEEVSG----QSGKLA 552

Query: 544 KLVDMTEMCKGPQDPPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 587
           K+VD +EM KGPQD PGHWLVTGAKLGVEKGKIVLRVKYSLLNY
Sbjct: 553 KIVDSSEMLKGPQDLPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 561

BLAST of Spg013144 vs. ExPASy Swiss-Prot
Match: Q9STW5 (MACPF domain-containing protein At4g24290 OS=Arabidopsis thaliana OX=3702 GN=At4g24290 PE=2 SV=1)

HSP 1 Score: 414.5 bits (1064), Expect = 2.1e-114
Identity = 248/603 (41.13%), Postives = 353/603 (58.54%), Query Frame = 0

Query: 14  AVQALGRGFDVNFDTRLLYCKGVAGST----VVEVDDKHTRDLRLYDDVVLLNVSRDIKN 73
           A+ ++G G+D+  D RL YCKG +  +    + E DD    ++ L   + + NVS+ IK 
Sbjct: 14  AIGSIGCGYDLAIDLRLKYCKGGSKDSRLLDIKEGDD--NCEIVLPGGISIPNVSKSIK- 73

Query: 74  FQDQEPVGHRSSGICSFDEMVDYFNQRDGLSEGYSSGSFNSAFGFTGSKQTDAVATKSLS 133
             D+       S I  F +M + FNQ   L+    SG FN+ F F+   Q DA  TK+L+
Sbjct: 74  -CDKGERMRFRSDILPFQQMAEQFNQELSLAGKIPSGLFNAMFEFSSCWQKDAAYTKNLA 133

Query: 134 TDGFYIPLAKFQLTNTPFVLQENVKRAVPTSWDPPSLASFIENFGTHVITSITIGGKDVI 193
            DG +I L    L  +  +L+E+VK+AVP++WDP +LA FI+ +GTH+I S+ +GGKDVI
Sbjct: 134 FDGVFISLYSVALDKSQVLLREHVKQAVPSTWDPAALARFIDIYGTHIIVSVKMGGKDVI 193

Query: 194 YVKQHQSSPLSTMEVKRYVQDIANQRF--------SGTQSQTGSGSIKLKEKGLDGGLFN 253
           Y KQ  SS L   ++++ ++++A++RF        +G++    S  ++ KE+ L     +
Sbjct: 194 YAKQQHSSKLQPEDLQKRLKEVADKRFVEASVVHNTGSERVQASSKVETKEQRLRFADTS 253

Query: 254 NQGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQ-NHVRWARTVRSSPDVIEMTFYPITA 313
           + G Y           KED   + +RRGG+D     H  W +TV+  PDVI M+F PIT+
Sbjct: 254 SLGSY---------ANKEDYVFMCKRRGGNDNRNLMHNEWLQTVQMEPDVISMSFIPITS 313

Query: 314 LLDGVAGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPVRDTIP-GHQRKEPVCPS 373
           LL+GV G   L+ AI LYL YKPP+EEL  FLEFQ+PR WAPV   +P G QRK+  C S
Sbjct: 314 LLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPVFSELPLGPQRKQQSCAS 373

Query: 374 LQFSIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKFLQPYWDTH 433
           LQFS  G KLYV+   + +G++P+TGMRL LEG + NRL+IHLQHL+SLPK  Q   D +
Sbjct: 374 LQFSFFGPKLYVNTTPVDVGKRPITGMRLYLEGRRSNRLAIHLQHLSSLPKIYQLEDDLN 433

Query: 434 VAIGAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWD 493
            +I        E  D R++E V WKN+SHV T P+E+ +    +LS   +VTGAQL V  
Sbjct: 434 RSI------RQESHDRRYYEKVNWKNYSHVCTEPVESDD----DLS---VVTGAQLHVES 493

Query: 494 FGSRNVLYMKLLYSRLPGCT-IRRTLWD------------------HLPNDKSQKLVSSS 553
            G +NVL+++L +SR+ G T ++ + WD                  H    +      + 
Sbjct: 494 HGFKNVLFLRLCFSRVVGATLVKNSEWDEAVGFAPKSGLISTLISHHFTAAQKPPPRPAD 553

Query: 554 VNSNNADDSSSGSNSIQGNKLAKLVDMTEMCKGPQDPPGHWLVTGAKLGVEKGKIVLRVK 584
           VN N+A          Q  KL K VD +EM +GPQ+ PG+W+V+GA+L VEKGKI L+VK
Sbjct: 554 VNINSAIYPGGPPVPTQAPKLLKFVDTSEMTRGPQESPGYWVVSGARLLVEKGKISLKVK 590

BLAST of Spg013144 vs. ExPASy Swiss-Prot
Match: Q9SGN6 (MACPF domain-containing protein NSL1 OS=Arabidopsis thaliana OX=3702 GN=NSL1 PE=2 SV=1)

HSP 1 Score: 376.3 bits (965), Expect = 6.2e-103
Identity = 234/601 (38.94%), Postives = 331/601 (55.07%), Query Frame = 0

Query: 11  AINAVQALGRGFDVNFDTRLLYCKGVA-GSTVVEVDDKHTRDLRLYDDVVLLNVSRDIKN 70
           A  AV  +G G+D+  D R   CK    GS +VE+D    RDL     +V+ NVS  IK 
Sbjct: 15  AEKAVSVIGLGYDLCSDVRFSACKTTPDGSRLVEIDPTRNRDLIFPGGIVVNNVSSSIK- 74

Query: 71  FQDQEPVGHRSSGICSFDEMVDYFNQRDGLSEGYSSGSFNSAFGFTGSKQTDAVATKSLS 130
             D+       S I SF++M + FNQ   LS    SG FN+ F F+     DA + K+L+
Sbjct: 75  -CDKGERTRLRSDILSFNQMSEKFNQDMCLSGKIPSGMFNNMFAFSKCWPKDASSVKTLA 134

Query: 131 TDGFYIPLAKFQLTNTPFVLQENVKRAVPTSWDPPSLASFIENFGTHVITSITIGGKDVI 190
            DG++I L   ++      L++ VKR VP+SWD  +LA FIE +GTHV+  +T+GGKDVI
Sbjct: 135 YDGWFISLYSVEIVRKQLTLRDEVKREVPSSWDSAALAGFIEKYGTHVVVGVTMGGKDVI 194

Query: 191 YVKQHQSSPLSTMEVKRYVQDIANQRFSGTQSQTGSGSIKLKEKGLDGGLFNNQGVYPQP 250
           +VKQ + S     E+++ ++   ++RF     ++ S +     K  +  L    G+ P  
Sbjct: 195 HVKQMRKSNHEPEEIQKMLKHWGDERFCVDPVESKSPASVYSGKPKEENLL-QWGLQPFG 254

Query: 251 TSAPYL----TGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFYPITALLDGV 310
           TS        T  E++  +  RRGG DL Q+H RW  TV  +P+VI M F PIT+LL G+
Sbjct: 255 TSVSSAVVMHTKNEEIMRVCIRRGGVDLGQSHERWLSTVSQAPNVISMCFVPITSLLSGL 314

Query: 311 AGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPVRDTIP-GHQRKEPVCPSLQFSI 370
            G   L+ A+ LYL YKPP+EEL  FLEFQ+PR WAPV   +P G +R +   PSLQFS+
Sbjct: 315 PGTGFLSHAVNLYLRYKPPIEELHQFLEFQLPRQWAPVYGDLPLGLRRSKQSSPSLQFSL 374

Query: 371 MGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKFLQ-PYWDTHVAIG 430
           MG KLYV+  ++  G +PVTG+R  LEG K N L+IHLQHL++ P  L   + DT+  I 
Sbjct: 375 MGPKLYVNTSKVDSGERPVTGLRFFLEGKKGNHLAIHLQHLSACPPSLHLSHDDTYEPIE 434

Query: 431 APKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFGSR 490
            P  +G       ++ PVKW  FSHV T P++       + +   IVT A L V   G R
Sbjct: 435 EPVEKG-------YYVPVKWGIFSHVCTYPVQYNGARSDDTAS--IVTKAWLEVKGMGMR 494

Query: 491 NVLYMKLLYSRLPGCTIRRTLWDHLPNDKSQKLVSSSVNSN------------------- 550
            VL+++L +S       R++ WD+L  +  +  V S +++                    
Sbjct: 495 KVLFLRLGFSLDASAVTRKSCWDNLSTNSRKSGVFSMISTRLSTGLSPNPATTKPQSKID 554

Query: 551 -NADDSSSGSNSIQGNKLAKLVDMTEMCKGPQDPPGHWLVTGAKLGVEKGKIVLRVKYSL 585
            N+     G +     KL  LVD  E+ +GP++ PG+W+VTGAKL VE GKI ++ KYSL
Sbjct: 555 INSAVYPRGPSPPVKPKLLSLVDTKEVMRGPEEQPGYWVVTGAKLCVEAGKISIKAKYSL 603

BLAST of Spg013144 vs. ExPASy Swiss-Prot
Match: Q8L612 (MACPF domain-containing protein At1g14780 OS=Arabidopsis thaliana OX=3702 GN=At1g14780 PE=2 SV=1)

HSP 1 Score: 369.4 bits (947), Expect = 7.6e-101
Identity = 230/616 (37.34%), Postives = 332/616 (53.90%), Query Frame = 0

Query: 14  AVQALGRGFDVNFDTRLLYCKGVAGST----VVEVDDKHTRDLRLYDDVVLLNVSRDIKN 73
           AV++LG+GFD+  D RL YCK   GS     +V +D    R+L +    V  NVS DI N
Sbjct: 12  AVKSLGKGFDLTADFRLKYCKDGDGSAGDDRLVVLDQTQNRELHIPGFGVFQNVSADI-N 71

Query: 74  FQDQEPVGHRSSGICSFDEMVDYFNQRDGLSEGYSSGSFNSAFGF-TGSKQTDAVATKSL 133
               E    RS  I  F++M +YFNQR  ++    SG+FN+ FGF +GS  TDA   KSL
Sbjct: 72  CDKGERTRFRSD-ILDFNKMSEYFNQRSSVTGKIPSGNFNATFGFQSGSWATDAANVKSL 131

Query: 134 STDGFYIPLAKFQLTN-TPFVLQENVKRAVPTSWDPPSLASFIENFGTHVITSITIGGKD 193
             D   + L    + N     L + V+ AVP+SWDP  LA FIE +GTHVIT +++GG+D
Sbjct: 132 GLDASVVTLFNLHIHNPNRLRLTDRVRNAVPSSWDPQLLARFIERYGTHVITGVSVGGQD 191

Query: 194 VIYVKQHQSSPLSTMEVKRYVQDIANQRFSGTQSQTGSGSIKLKEKGLDGGLFNNQ-GVY 253
           V+ V+Q +SS L    ++ ++ D+ +Q F+G+   +     K          F     V+
Sbjct: 192 VVVVRQDKSSDLDNDLLRHHLYDLGDQLFTGSCLLSTRRLNKAYHHSHSQPKFPEAFNVF 251

Query: 254 PQPTSAPY----LTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFYPITALL 313
               +  +    +  +  +TVI  +RGGD   ++H  W  TV   PD I   F PIT+LL
Sbjct: 252 DDKQTVAFNNFSINSQNGITVICAKRGGDGRAKSHSEWLITVPDKPDAINFNFIPITSLL 311

Query: 314 DGVAGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPVRDTIP--GHQRKEPVCPSL 373
             V G   L+ A+ LYL YKPPL +L+YFL+F  PR WAPV + +P           P+L
Sbjct: 312 KDVPGSGLLSHAMSLYLRYKPPLMDLQYFLDFSGPRAWAPVHNDLPFGAAPNMASAYPAL 371

Query: 374 QFSIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKFL-QPYWDTH 433
             + MG KLYV+   ++  + PVTGMR  LEG K NRL+IHLQHL +    + +   D H
Sbjct: 372 HINFMGPKLYVNTTPVTSEKNPVTGMRFFLEGKKCNRLAIHLQHLDNTRTTVGEKITDEH 431

Query: 434 VAIGAPKWQGPEE--QDSRWFEPVKWKNFSHVSTAPIENPETFMGELSG-------VYIV 493
           +      W+G ++   + R+FEP+  K FSHV T P++    ++   S         +IV
Sbjct: 432 I------WRGSDQITDNDRYFEPLNGKKFSHVCTVPVKYDPNWIKTTSNHKSQNDVAFIV 491

Query: 494 TGAQLGVWDFGSRNVLYMKLLYSRLPGCTIRRTLWDHLPNDKSQK----------LVSSS 553
           TGAQL V   GS++VL+++L Y+++    + +  W H P   SQK          L S S
Sbjct: 492 TGAQLEVKKHGSKSVLHLRLRYTKVSDHYVVQNSWVHGPIGTSQKSGIFSSMSMPLTSGS 551

Query: 554 VNSNNADDSSS-----------GSNSIQGNKLAKLVDMTEMCKGPQDPPGHWLVTGAKLG 586
           V+ N      +           G      NK+ K VD++++C+GPQ  PGHWLVTG +L 
Sbjct: 552 VHHNMIQKDKNEVVLDSGVFPGGPPVPANNKIVKFVDLSQLCRGPQHSPGHWLVTGVRLY 611

BLAST of Spg013144 vs. ExPASy TrEMBL
Match: A0A6J1CR77 (MACPF domain-containing protein CAD1-like OS=Momordica charantia OX=3673 GN=LOC111013848 PE=4 SV=1)

HSP 1 Score: 1102.8 bits (2851), Expect = 0.0e+00
Identity = 541/586 (92.32%), Postives = 559/586 (95.39%), Query Frame = 0

Query: 1   MGESGTALHTAINAVQALGRGFDVNFDTRLLYCKGVAGSTVVEVDDKHTRDLRLYDDVVL 60
           MG++GTALHTAINAVQALGRGFDVNFDTRLLYCKGV+GST+VEVDD+HTRD+ LYDDVVL
Sbjct: 1   MGDTGTALHTAINAVQALGRGFDVNFDTRLLYCKGVSGSTIVEVDDEHTRDICLYDDVVL 60

Query: 61  LNVSRDIKNFQDQEPVGHRSSGICSFDEMVDYFNQRDGLSEGYSSGSFNSAFGFTGSKQT 120
           LNVS DIKNF  QEP+GHRSSGICSFDEMV+YFNQ+DGLSEGY+SGSFNSAFGF GSKQ 
Sbjct: 61  LNVSWDIKNF--QEPIGHRSSGICSFDEMVNYFNQKDGLSEGYTSGSFNSAFGFNGSKQI 120

Query: 121 DAVATKSLSTDGFYIPLAKFQLTNTPFVLQENVKRAVPTSWDPPSLASFIENFGTHVITS 180
           DA  TK+LS DGFYIPLAKFQ T TP VLQENVKRA+PTSWDPPSLASFIENFGTHVITS
Sbjct: 121 DAATTKNLSMDGFYIPLAKFQFTKTPLVLQENVKRAIPTSWDPPSLASFIENFGTHVITS 180

Query: 181 ITIGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSGTQSQTGSGSIKLKEKGLDGGLF 240
           ITIGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFS TQ  TGSGSIKLKEKGLDGGLF
Sbjct: 181 ITIGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGLTGSGSIKLKEKGLDGGLF 240

Query: 241 NNQGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFYPITA 300
           NNQGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVR SPDVIEMTF+PITA
Sbjct: 241 NNQGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRLSPDVIEMTFFPITA 300

Query: 301 LLDGVAGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPVRDTIPGHQRKEPVCPSL 360
           LLDGV GKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPVRDT+PGHQRKEPVCPSL
Sbjct: 301 LLDGVTGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPVRDTVPGHQRKEPVCPSL 360

Query: 361 QFSIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKFLQPYWDTHV 420
           QFSIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPK LQPYWDTHV
Sbjct: 361 QFSIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKILQPYWDTHV 420

Query: 421 AIGAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDF 480
            IGAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDF
Sbjct: 421 GIGAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDF 480

Query: 481 GSRNVLYMKLLYSRLPGCTIRRTLWDHLPNDKSQKLVSSSVNSNNADDSSSGSNSIQGNK 540
           G RNVLYMKLLYSRLPGCTI+RTLWDHL NDKS+KLVSSS NS NADDSSSGS +IQGNK
Sbjct: 481 GPRNVLYMKLLYSRLPGCTIQRTLWDHLLNDKSKKLVSSSDNS-NADDSSSGSRNIQGNK 540

Query: 541 LAKLVDMTEMCKGPQDPPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 587
           LAKLVD +EM KGP DPPGHWLVTGAKLGVEKGKIVLRVKYSLLNY
Sbjct: 541 LAKLVDTSEMSKGPHDPPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 583

BLAST of Spg013144 vs. ExPASy TrEMBL
Match: A0A5D3CHB3 (MACPF domain-containing protein CAD1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold127G00610 PE=4 SV=1)

HSP 1 Score: 1092.0 bits (2823), Expect = 0.0e+00
Identity = 539/588 (91.67%), Postives = 557/588 (94.73%), Query Frame = 0

Query: 1   MGESGTALHTAINAVQALGRGFDVNFDTRLLYCKGVAGSTVVEVDDKHTRDLRLYDDVVL 60
           MGESGTALHTA NAVQALGRGFDVNFDTRLLYCKGVAGST+VEVDD+H+RD+ LYDDVVL
Sbjct: 1   MGESGTALHTASNAVQALGRGFDVNFDTRLLYCKGVAGSTIVEVDDEHSRDICLYDDVVL 60

Query: 61  LNVSRDIKNFQDQEPVGHRSSGICSFDEMVDYFNQRDGLSEGYSSGSFNSAFGFTGSKQT 120
            NVS DIKNF  QEP+GHRSSGICSFDEM DYFNQ+DGLSEGY SGSFNSAFGFTG +QT
Sbjct: 61  HNVSWDIKNF--QEPIGHRSSGICSFDEMADYFNQKDGLSEGYPSGSFNSAFGFTGLRQT 120

Query: 121 DAVATKSLSTDGFYIPLAKFQLTNTPFVLQENVKRAVPTSWDPPSLASFIENFGTHVITS 180
           DA ATKSLSTDGFYIPLAKFQ T TP  L+EN+KRAVPT+WDPPSLASFIENFGTHVITS
Sbjct: 121 DASATKSLSTDGFYIPLAKFQFTKTPLELKENIKRAVPTTWDPPSLASFIENFGTHVITS 180

Query: 181 ITIGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSGTQSQTGSGSIKLKEKGLDGGLF 240
           ITIGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFS TQ QTGSGSIKLKEKGLDGGLF
Sbjct: 181 ITIGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGLDGGLF 240

Query: 241 NNQGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFYPITA 300
           NNQGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFYPITA
Sbjct: 241 NNQGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFYPITA 300

Query: 301 LLDGVAGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPVRDTIPGHQRKEPVCPSL 360
           LLDGV GKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAP+ D +PG QRKEPVCPSL
Sbjct: 301 LLDGVVGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPIHDKLPGRQRKEPVCPSL 360

Query: 361 QFSIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKFLQPYWDTHV 420
           QFSIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPK + PYWDTHV
Sbjct: 361 QFSIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMHPYWDTHV 420

Query: 421 AIGAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDF 480
           AIGAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDF
Sbjct: 421 AIGAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDF 480

Query: 481 GSRNVLYMKLLYSRLPGCTIRRTLWDHLPN-DKSQKLVSSSVNSNNADDSSSGSNSIQG- 540
           GSRNVLYMKLLYSRLPGCTIRRTLWDHLP  +KS+KLVSSS N+NNAD+SSSG+ S QG 
Sbjct: 481 GSRNVLYMKLLYSRLPGCTIRRTLWDHLPPIEKSKKLVSSSDNTNNADNSSSGTKSTQGN 540

Query: 541 NKLAKLVDMTEMCKGPQDPPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 587
           NKLAKLVD TEM KGPQD PGHWLVTGAKLGVEKGKIVLRVKYSLLNY
Sbjct: 541 NKLAKLVDTTEMSKGPQDSPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 586

BLAST of Spg013144 vs. ExPASy TrEMBL
Match: A0A1S3CJ73 (MACPF domain-containing protein CAD1-like OS=Cucumis melo OX=3656 GN=LOC103501463 PE=4 SV=1)

HSP 1 Score: 1092.0 bits (2823), Expect = 0.0e+00
Identity = 539/588 (91.67%), Postives = 557/588 (94.73%), Query Frame = 0

Query: 1   MGESGTALHTAINAVQALGRGFDVNFDTRLLYCKGVAGSTVVEVDDKHTRDLRLYDDVVL 60
           MGESGTALHTA NAVQALGRGFDVNFDTRLLYCKGVAGST+VEVDD+H+RD+ LYDDVVL
Sbjct: 1   MGESGTALHTASNAVQALGRGFDVNFDTRLLYCKGVAGSTIVEVDDEHSRDICLYDDVVL 60

Query: 61  LNVSRDIKNFQDQEPVGHRSSGICSFDEMVDYFNQRDGLSEGYSSGSFNSAFGFTGSKQT 120
            NVS DIKNF  QEP+GHRSSGICSFDEM DYFNQ+DGLSEGY SGSFNSAFGFTG +QT
Sbjct: 61  HNVSWDIKNF--QEPIGHRSSGICSFDEMADYFNQKDGLSEGYPSGSFNSAFGFTGLRQT 120

Query: 121 DAVATKSLSTDGFYIPLAKFQLTNTPFVLQENVKRAVPTSWDPPSLASFIENFGTHVITS 180
           DA ATKSLSTDGFYIPLAKFQ T TP  L+EN+KRAVPT+WDPPSLASFIENFGTHVITS
Sbjct: 121 DASATKSLSTDGFYIPLAKFQFTKTPLELKENIKRAVPTTWDPPSLASFIENFGTHVITS 180

Query: 181 ITIGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSGTQSQTGSGSIKLKEKGLDGGLF 240
           ITIGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFS TQ QTGSGSIKLKEKGLDGGLF
Sbjct: 181 ITIGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGLDGGLF 240

Query: 241 NNQGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFYPITA 300
           NNQGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFYPITA
Sbjct: 241 NNQGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFYPITA 300

Query: 301 LLDGVAGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPVRDTIPGHQRKEPVCPSL 360
           LLDGV GKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAP+ D +PG QRKEPVCPSL
Sbjct: 301 LLDGVVGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPIHDKLPGRQRKEPVCPSL 360

Query: 361 QFSIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKFLQPYWDTHV 420
           QFSIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPK + PYWDTHV
Sbjct: 361 QFSIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMHPYWDTHV 420

Query: 421 AIGAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDF 480
           AIGAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDF
Sbjct: 421 AIGAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDF 480

Query: 481 GSRNVLYMKLLYSRLPGCTIRRTLWDHLPN-DKSQKLVSSSVNSNNADDSSSGSNSIQG- 540
           GSRNVLYMKLLYSRLPGCTIRRTLWDHLP  +KS+KLVSSS N+NNAD+SSSG+ S QG 
Sbjct: 481 GSRNVLYMKLLYSRLPGCTIRRTLWDHLPPIEKSKKLVSSSDNTNNADNSSSGTKSTQGN 540

Query: 541 NKLAKLVDMTEMCKGPQDPPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 587
           NKLAKLVD TEM KGPQD PGHWLVTGAKLGVEKGKIVLRVKYSLLNY
Sbjct: 541 NKLAKLVDTTEMSKGPQDSPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 586

BLAST of Spg013144 vs. ExPASy TrEMBL
Match: A0A6J1EBB6 (MACPF domain-containing protein CAD1-like OS=Cucurbita moschata OX=3662 GN=LOC111432562 PE=4 SV=1)

HSP 1 Score: 1088.2 bits (2813), Expect = 0.0e+00
Identity = 536/587 (91.31%), Postives = 556/587 (94.72%), Query Frame = 0

Query: 1   MGESGTALHTAINAVQALGRGFDVNFDTRLLYCKGVAGSTVVEVDDKHTRDLRLYDDVVL 60
           M ESGTALHTAINAVQALGRGFDVNFDTRLLYCKGVAGST+VEVDD+HTRD+ LYDDVVL
Sbjct: 1   MEESGTALHTAINAVQALGRGFDVNFDTRLLYCKGVAGSTIVEVDDEHTRDICLYDDVVL 60

Query: 61  LNVSRDIKNFQDQEPVGHRSSGICSFDEMVDYFNQRDGLSEGYSSGSFNSAFGFTGSKQT 120
           LNVS DIKNF  Q+P+GHRSSGICSFDEMVDYFNQ+DGLSEGY SGSFN+AFGFTGSKQ 
Sbjct: 61  LNVSWDIKNF--QQPIGHRSSGICSFDEMVDYFNQKDGLSEGYPSGSFNTAFGFTGSKQI 120

Query: 121 DAVATKSLSTDGFYIPLAKFQLTNTPFVLQENVKRAVPTSWDPPSLASFIENFGTHVITS 180
           DA A K+LSTDGFYIPLAKFQLT+T  +L ENVKR+VPTSWDPP+LASFIENFGTHVITS
Sbjct: 121 DAAAAKNLSTDGFYIPLAKFQLTSTSLLLHENVKRSVPTSWDPPALASFIENFGTHVITS 180

Query: 181 ITIGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSGTQSQTGSGSIKLKEKGLDGGLF 240
           ITIGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFS TQ Q GSGS KLKEKGLDGGLF
Sbjct: 181 ITIGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQAGSGSRKLKEKGLDGGLF 240

Query: 241 NNQGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFYPITA 300
           NNQGVYPQPTSAPY +GKEDVTVIFRRRGGDDLEQNHV+WA+TVRSSPDVIEMTFYPITA
Sbjct: 241 NNQGVYPQPTSAPYPSGKEDVTVIFRRRGGDDLEQNHVKWAKTVRSSPDVIEMTFYPITA 300

Query: 301 LLDGVAGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPVRDTIPGHQRKEPVCPSL 360
           LLDGVAGKEHLTRAI LYLEYKPPLEELRYFLEFQIPRVWAPVRDTIPGHQRKEPVCPSL
Sbjct: 301 LLDGVAGKEHLTRAIRLYLEYKPPLEELRYFLEFQIPRVWAPVRDTIPGHQRKEPVCPSL 360

Query: 361 QFSIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKFLQPYWDTHV 420
           QFSIMGQKLYVSIEQI IGRKPVTGM LSLEG KQNRLSIHLQHLA+LPK LQPYWDTH+
Sbjct: 361 QFSIMGQKLYVSIEQILIGRKPVTGMCLSLEGGKQNRLSIHLQHLATLPKILQPYWDTHI 420

Query: 421 AIGAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDF 480
           AIGAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVW F
Sbjct: 421 AIGAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWGF 480

Query: 481 GSRNVLYMKLLYSRLPGCTIRRTLWDHLPNDKSQKLVSSSVNSN-NADDSSSGSNSIQGN 540
           GSRNVLYMKLLYSRLPGCTIRRTLWDHLPNDKS+KLVSSS NSN  ADDSSSGS +IQGN
Sbjct: 481 GSRNVLYMKLLYSRLPGCTIRRTLWDHLPNDKSKKLVSSSDNSNAAADDSSSGSKNIQGN 540

Query: 541 KLAKLVDMTEMCKGPQDPPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 587
           KLAKLVD TEM KGPQD PGHWLVTGAKLGVEK +IVLRVKYSLLNY
Sbjct: 541 KLAKLVDTTEMSKGPQDSPGHWLVTGAKLGVEKSRIVLRVKYSLLNY 585

BLAST of Spg013144 vs. ExPASy TrEMBL
Match: A0A6J1HR44 (MACPF domain-containing protein CAD1-like OS=Cucurbita maxima OX=3661 GN=LOC111465969 PE=4 SV=1)

HSP 1 Score: 1082.0 bits (2797), Expect = 0.0e+00
Identity = 533/587 (90.80%), Postives = 555/587 (94.55%), Query Frame = 0

Query: 1   MGESGTALHTAINAVQALGRGFDVNFDTRLLYCKGVAGSTVVEVDDKHTRDLRLYDDVVL 60
           M ESGTALHTAINAVQALGRGFDVNFDTRLLYCKGVAGST+VEVDD+HTRD+ LYDDVVL
Sbjct: 1   MEESGTALHTAINAVQALGRGFDVNFDTRLLYCKGVAGSTIVEVDDEHTRDICLYDDVVL 60

Query: 61  LNVSRDIKNFQDQEPVGHRSSGICSFDEMVDYFNQRDGLSEGYSSGSFNSAFGFTGSKQT 120
           LNVS DIKN   Q+P+GHRSSGICSFDEMVDYFNQ+DGLSEGY SGSFN+AFGFTGSKQ 
Sbjct: 61  LNVSWDIKN--SQQPIGHRSSGICSFDEMVDYFNQKDGLSEGYPSGSFNTAFGFTGSKQI 120

Query: 121 DAVATKSLSTDGFYIPLAKFQLTNTPFVLQENVKRAVPTSWDPPSLASFIENFGTHVITS 180
           DA A K+LSTDGFYIPLAKFQLT+T  +L ENVKR+VPTSWDPP+LASFIENFGTHVITS
Sbjct: 121 DADAAKNLSTDGFYIPLAKFQLTSTSLLLHENVKRSVPTSWDPPALASFIENFGTHVITS 180

Query: 181 ITIGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSGTQSQTGSGSIKLKEKGLDGGLF 240
           ITIGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFS TQ Q GSGS KLKEKGLDGGLF
Sbjct: 181 ITIGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQAGSGSRKLKEKGLDGGLF 240

Query: 241 NNQGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFYPITA 300
           NNQGVYPQPTSAPY +GKEDVTVIFRRRGGDDLEQNHV+WA+TVRSSPDVIEMTFYPIT+
Sbjct: 241 NNQGVYPQPTSAPYPSGKEDVTVIFRRRGGDDLEQNHVKWAKTVRSSPDVIEMTFYPITS 300

Query: 301 LLDGVAGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPVRDTIPGHQRKEPVCPSL 360
           LLDGVAGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPVRDTIPGHQRKEPVCPSL
Sbjct: 301 LLDGVAGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPVRDTIPGHQRKEPVCPSL 360

Query: 361 QFSIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKFLQPYWDTHV 420
           QFSIMGQKLYVSIEQI IGRKPVTGM LSLEG KQNRLSIHL+HLA+LPK LQPYWDTH+
Sbjct: 361 QFSIMGQKLYVSIEQILIGRKPVTGMCLSLEGGKQNRLSIHLKHLATLPKILQPYWDTHI 420

Query: 421 AIGAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDF 480
           AIGAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVW F
Sbjct: 421 AIGAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWGF 480

Query: 481 GSRNVLYMKLLYSRLPGCTIRRTLWDHLPNDKSQKLVSSSVNSN-NADDSSSGSNSIQGN 540
           GSRNVLYMKLLYSRLPGCTIRRTLWDHLPNDKS+KLVSSS NSN  ADDSSSGS + QGN
Sbjct: 481 GSRNVLYMKLLYSRLPGCTIRRTLWDHLPNDKSKKLVSSSDNSNYAADDSSSGSKNTQGN 540

Query: 541 KLAKLVDMTEMCKGPQDPPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 587
           KLAKLVD TEM KGPQD PGHWLVTGAKLGVEK +IVLRVKYSLLNY
Sbjct: 541 KLAKLVDTTEMSKGPQDSPGHWLVTGAKLGVEKSRIVLRVKYSLLNY 585

BLAST of Spg013144 vs. TAIR 10
Match: AT1G29690.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 701.4 bits (1809), Expect = 6.0e-202
Identity = 345/584 (59.08%), Postives = 430/584 (73.63%), Query Frame = 0

Query: 4   SGTALHTAI-NAVQALGRGFDVNFDTRLLYCKGVAGSTVVEVDDKHTRDLRLYDDVVLLN 63
           S  AL T + NA+QALGRGFDV  D RLLYCKG  GS +V +++   RDL L    +L N
Sbjct: 13  SSEALTTTLRNAIQALGRGFDVTSDVRLLYCKGAPGSRLVRIEEGQNRDLELSHGFLLPN 72

Query: 64  VSRDIKNFQDQEPVGHRSSGICSFDEMVDYFNQRDGLSEGYSSGSFNSAFGFTGSKQTDA 123
           V  DI   +     G +   +CSF EM + FN R G+      G FN+ F +TGS Q DA
Sbjct: 73  VPADIDCSRGNS--GTQRISVCSFHEMAEEFNVRSGVKGNIPLGCFNAMFNYTGSWQVDA 132

Query: 124 VATKSLSTDGFYIPLAKFQLTNTPFVLQENVKRAVPTSWDPPSLASFIENFGTHVITSIT 183
            +TKSL+  G++IPL   +L     VL   ++RAVP+SWDP SLASFIEN+GTH++TS+T
Sbjct: 133 ASTKSLALVGYFIPLYDVKLAKLTLVLHNEIRRAVPSSWDPASLASFIENYGTHIVTSVT 192

Query: 184 IGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSGTQSQTGSGSIKLKEKGLDGGLFNN 243
           IGG+DV+Y++QHQSSPL   E++ YV D+   RF   +SQ+ +G +K K+K         
Sbjct: 193 IGGRDVVYIRQHQSSPLPVSEIENYVNDMIKHRFHEAESQSITGPLKYKDK--------- 252

Query: 244 QGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFYPITALL 303
                            D+TVIFRRRGGDDLEQ+H RWA TV ++PD+I MTF PI +LL
Sbjct: 253 -----------------DITVIFRRRGGDDLEQSHARWAETVPAAPDIINMTFTPIVSLL 312

Query: 304 DGVAGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPVRDTIPGHQRKEPVCPSLQF 363
           +GV G  HLTRAI LYLEYKPP+E+L+YFL++QI R WAP +  +   QRKEPVC SLQF
Sbjct: 313 EGVPGLRHLTRAIELYLEYKPPIEDLQYFLDYQIARAWAPEQSNL---QRKEPVCSSLQF 372

Query: 364 SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKFLQPYWDTHVAI 423
           S+MG KL++S +Q+++GRKPVTG+RLSLEGSKQNRLSIHLQHL SLPK LQP+WD+HV I
Sbjct: 373 SLMGPKLFISADQVTVGRKPVTGLRLSLEGSKQNRLSIHLQHLVSLPKILQPHWDSHVPI 432

Query: 424 GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFGS 483
           GAPKWQGPEEQDSRWFEP+KWKNFSHVST+PIE+ ET +G+LSGV+IVTGAQLGVWDFGS
Sbjct: 433 GAPKWQGPEEQDSRWFEPIKWKNFSHVSTSPIEHTETHIGDLSGVHIVTGAQLGVWDFGS 492

Query: 484 RNVLYMKLLYSRLPGCTIRRTLWDHLPNDKSQKLVSSSVNSNNADDSSSGSNSIQGNKLA 543
           +NVL++KLL+S++PGCTIRR++WDH P   S +L     +++++ +  SG    Q  KLA
Sbjct: 493 KNVLHLKLLFSKVPGCTIRRSVWDHTPVASSGRLEPGGPSTSSSTEEVSG----QSGKLA 552

Query: 544 KLVDMTEMCKGPQDPPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 587
           K+VD +EM KGPQD PGHWLVTGAKLGVEKGKIVLRVKYSLLNY
Sbjct: 553 KIVDSSEMLKGPQDLPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 561

BLAST of Spg013144 vs. TAIR 10
Match: AT4G24290.2 (MAC/Perforin domain-containing protein )

HSP 1 Score: 414.5 bits (1064), Expect = 1.5e-115
Identity = 248/603 (41.13%), Postives = 353/603 (58.54%), Query Frame = 0

Query: 14  AVQALGRGFDVNFDTRLLYCKGVAGST----VVEVDDKHTRDLRLYDDVVLLNVSRDIKN 73
           A+ ++G G+D+  D RL YCKG +  +    + E DD    ++ L   + + NVS+ IK 
Sbjct: 14  AIGSIGCGYDLAIDLRLKYCKGGSKDSRLLDIKEGDD--NCEIVLPGGISIPNVSKSIK- 73

Query: 74  FQDQEPVGHRSSGICSFDEMVDYFNQRDGLSEGYSSGSFNSAFGFTGSKQTDAVATKSLS 133
             D+       S I  F +M + FNQ   L+    SG FN+ F F+   Q DA  TK+L+
Sbjct: 74  -CDKGERMRFRSDILPFQQMAEQFNQELSLAGKIPSGLFNAMFEFSSCWQKDAAYTKNLA 133

Query: 134 TDGFYIPLAKFQLTNTPFVLQENVKRAVPTSWDPPSLASFIENFGTHVITSITIGGKDVI 193
            DG +I L    L  +  +L+E+VK+AVP++WDP +LA FI+ +GTH+I S+ +GGKDVI
Sbjct: 134 FDGVFISLYSVALDKSQVLLREHVKQAVPSTWDPAALARFIDIYGTHIIVSVKMGGKDVI 193

Query: 194 YVKQHQSSPLSTMEVKRYVQDIANQRF--------SGTQSQTGSGSIKLKEKGLDGGLFN 253
           Y KQ  SS L   ++++ ++++A++RF        +G++    S  ++ KE+ L     +
Sbjct: 194 YAKQQHSSKLQPEDLQKRLKEVADKRFVEASVVHNTGSERVQASSKVETKEQRLRFADTS 253

Query: 254 NQGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQ-NHVRWARTVRSSPDVIEMTFYPITA 313
           + G Y           KED   + +RRGG+D     H  W +TV+  PDVI M+F PIT+
Sbjct: 254 SLGSY---------ANKEDYVFMCKRRGGNDNRNLMHNEWLQTVQMEPDVISMSFIPITS 313

Query: 314 LLDGVAGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPVRDTIP-GHQRKEPVCPS 373
           LL+GV G   L+ AI LYL YKPP+EEL  FLEFQ+PR WAPV   +P G QRK+  C S
Sbjct: 314 LLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPVFSELPLGPQRKQQSCAS 373

Query: 374 LQFSIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKFLQPYWDTH 433
           LQFS  G KLYV+   + +G++P+TGMRL LEG + NRL+IHLQHL+SLPK  Q   D +
Sbjct: 374 LQFSFFGPKLYVNTTPVDVGKRPITGMRLYLEGRRSNRLAIHLQHLSSLPKIYQLEDDLN 433

Query: 434 VAIGAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWD 493
            +I        E  D R++E V WKN+SHV T P+E+ +    +LS   +VTGAQL V  
Sbjct: 434 RSI------RQESHDRRYYEKVNWKNYSHVCTEPVESDD----DLS---VVTGAQLHVES 493

Query: 494 FGSRNVLYMKLLYSRLPGCT-IRRTLWD------------------HLPNDKSQKLVSSS 553
            G +NVL+++L +SR+ G T ++ + WD                  H    +      + 
Sbjct: 494 HGFKNVLFLRLCFSRVVGATLVKNSEWDEAVGFAPKSGLISTLISHHFTAAQKPPPRPAD 553

Query: 554 VNSNNADDSSSGSNSIQGNKLAKLVDMTEMCKGPQDPPGHWLVTGAKLGVEKGKIVLRVK 584
           VN N+A          Q  KL K VD +EM +GPQ+ PG+W+V+GA+L VEKGKI L+VK
Sbjct: 554 VNINSAIYPGGPPVPTQAPKLLKFVDTSEMTRGPQESPGYWVVSGARLLVEKGKISLKVK 590

BLAST of Spg013144 vs. TAIR 10
Match: AT1G28380.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 376.3 bits (965), Expect = 4.4e-104
Identity = 234/601 (38.94%), Postives = 331/601 (55.07%), Query Frame = 0

Query: 11  AINAVQALGRGFDVNFDTRLLYCKGVA-GSTVVEVDDKHTRDLRLYDDVVLLNVSRDIKN 70
           A  AV  +G G+D+  D R   CK    GS +VE+D    RDL     +V+ NVS  IK 
Sbjct: 15  AEKAVSVIGLGYDLCSDVRFSACKTTPDGSRLVEIDPTRNRDLIFPGGIVVNNVSSSIK- 74

Query: 71  FQDQEPVGHRSSGICSFDEMVDYFNQRDGLSEGYSSGSFNSAFGFTGSKQTDAVATKSLS 130
             D+       S I SF++M + FNQ   LS    SG FN+ F F+     DA + K+L+
Sbjct: 75  -CDKGERTRLRSDILSFNQMSEKFNQDMCLSGKIPSGMFNNMFAFSKCWPKDASSVKTLA 134

Query: 131 TDGFYIPLAKFQLTNTPFVLQENVKRAVPTSWDPPSLASFIENFGTHVITSITIGGKDVI 190
            DG++I L   ++      L++ VKR VP+SWD  +LA FIE +GTHV+  +T+GGKDVI
Sbjct: 135 YDGWFISLYSVEIVRKQLTLRDEVKREVPSSWDSAALAGFIEKYGTHVVVGVTMGGKDVI 194

Query: 191 YVKQHQSSPLSTMEVKRYVQDIANQRFSGTQSQTGSGSIKLKEKGLDGGLFNNQGVYPQP 250
           +VKQ + S     E+++ ++   ++RF     ++ S +     K  +  L    G+ P  
Sbjct: 195 HVKQMRKSNHEPEEIQKMLKHWGDERFCVDPVESKSPASVYSGKPKEENLL-QWGLQPFG 254

Query: 251 TSAPYL----TGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFYPITALLDGV 310
           TS        T  E++  +  RRGG DL Q+H RW  TV  +P+VI M F PIT+LL G+
Sbjct: 255 TSVSSAVVMHTKNEEIMRVCIRRGGVDLGQSHERWLSTVSQAPNVISMCFVPITSLLSGL 314

Query: 311 AGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPVRDTIP-GHQRKEPVCPSLQFSI 370
            G   L+ A+ LYL YKPP+EEL  FLEFQ+PR WAPV   +P G +R +   PSLQFS+
Sbjct: 315 PGTGFLSHAVNLYLRYKPPIEELHQFLEFQLPRQWAPVYGDLPLGLRRSKQSSPSLQFSL 374

Query: 371 MGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKFLQ-PYWDTHVAIG 430
           MG KLYV+  ++  G +PVTG+R  LEG K N L+IHLQHL++ P  L   + DT+  I 
Sbjct: 375 MGPKLYVNTSKVDSGERPVTGLRFFLEGKKGNHLAIHLQHLSACPPSLHLSHDDTYEPIE 434

Query: 431 APKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFGSR 490
            P  +G       ++ PVKW  FSHV T P++       + +   IVT A L V   G R
Sbjct: 435 EPVEKG-------YYVPVKWGIFSHVCTYPVQYNGARSDDTAS--IVTKAWLEVKGMGMR 494

Query: 491 NVLYMKLLYSRLPGCTIRRTLWDHLPNDKSQKLVSSSVNSN------------------- 550
            VL+++L +S       R++ WD+L  +  +  V S +++                    
Sbjct: 495 KVLFLRLGFSLDASAVTRKSCWDNLSTNSRKSGVFSMISTRLSTGLSPNPATTKPQSKID 554

Query: 551 -NADDSSSGSNSIQGNKLAKLVDMTEMCKGPQDPPGHWLVTGAKLGVEKGKIVLRVKYSL 585
            N+     G +     KL  LVD  E+ +GP++ PG+W+VTGAKL VE GKI ++ KYSL
Sbjct: 555 INSAVYPRGPSPPVKPKLLSLVDTKEVMRGPEEQPGYWVVTGAKLCVEAGKISIKAKYSL 603

BLAST of Spg013144 vs. TAIR 10
Match: AT1G14780.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 369.4 bits (947), Expect = 5.4e-102
Identity = 230/616 (37.34%), Postives = 332/616 (53.90%), Query Frame = 0

Query: 14  AVQALGRGFDVNFDTRLLYCKGVAGST----VVEVDDKHTRDLRLYDDVVLLNVSRDIKN 73
           AV++LG+GFD+  D RL YCK   GS     +V +D    R+L +    V  NVS DI N
Sbjct: 12  AVKSLGKGFDLTADFRLKYCKDGDGSAGDDRLVVLDQTQNRELHIPGFGVFQNVSADI-N 71

Query: 74  FQDQEPVGHRSSGICSFDEMVDYFNQRDGLSEGYSSGSFNSAFGF-TGSKQTDAVATKSL 133
               E    RS  I  F++M +YFNQR  ++    SG+FN+ FGF +GS  TDA   KSL
Sbjct: 72  CDKGERTRFRSD-ILDFNKMSEYFNQRSSVTGKIPSGNFNATFGFQSGSWATDAANVKSL 131

Query: 134 STDGFYIPLAKFQLTN-TPFVLQENVKRAVPTSWDPPSLASFIENFGTHVITSITIGGKD 193
             D   + L    + N     L + V+ AVP+SWDP  LA FIE +GTHVIT +++GG+D
Sbjct: 132 GLDASVVTLFNLHIHNPNRLRLTDRVRNAVPSSWDPQLLARFIERYGTHVITGVSVGGQD 191

Query: 194 VIYVKQHQSSPLSTMEVKRYVQDIANQRFSGTQSQTGSGSIKLKEKGLDGGLFNNQ-GVY 253
           V+ V+Q +SS L    ++ ++ D+ +Q F+G+   +     K          F     V+
Sbjct: 192 VVVVRQDKSSDLDNDLLRHHLYDLGDQLFTGSCLLSTRRLNKAYHHSHSQPKFPEAFNVF 251

Query: 254 PQPTSAPY----LTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFYPITALL 313
               +  +    +  +  +TVI  +RGGD   ++H  W  TV   PD I   F PIT+LL
Sbjct: 252 DDKQTVAFNNFSINSQNGITVICAKRGGDGRAKSHSEWLITVPDKPDAINFNFIPITSLL 311

Query: 314 DGVAGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPVRDTIP--GHQRKEPVCPSL 373
             V G   L+ A+ LYL YKPPL +L+YFL+F  PR WAPV + +P           P+L
Sbjct: 312 KDVPGSGLLSHAMSLYLRYKPPLMDLQYFLDFSGPRAWAPVHNDLPFGAAPNMASAYPAL 371

Query: 374 QFSIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKFL-QPYWDTH 433
             + MG KLYV+   ++  + PVTGMR  LEG K NRL+IHLQHL +    + +   D H
Sbjct: 372 HINFMGPKLYVNTTPVTSEKNPVTGMRFFLEGKKCNRLAIHLQHLDNTRTTVGEKITDEH 431

Query: 434 VAIGAPKWQGPEE--QDSRWFEPVKWKNFSHVSTAPIENPETFMGELSG-------VYIV 493
           +      W+G ++   + R+FEP+  K FSHV T P++    ++   S         +IV
Sbjct: 432 I------WRGSDQITDNDRYFEPLNGKKFSHVCTVPVKYDPNWIKTTSNHKSQNDVAFIV 491

Query: 494 TGAQLGVWDFGSRNVLYMKLLYSRLPGCTIRRTLWDHLPNDKSQK----------LVSSS 553
           TGAQL V   GS++VL+++L Y+++    + +  W H P   SQK          L S S
Sbjct: 492 TGAQLEVKKHGSKSVLHLRLRYTKVSDHYVVQNSWVHGPIGTSQKSGIFSSMSMPLTSGS 551

Query: 554 VNSNNADDSSS-----------GSNSIQGNKLAKLVDMTEMCKGPQDPPGHWLVTGAKLG 586
           V+ N      +           G      NK+ K VD++++C+GPQ  PGHWLVTG +L 
Sbjct: 552 VHHNMIQKDKNEVVLDSGVFPGGPPVPANNKIVKFVDLSQLCRGPQHSPGHWLVTGVRLY 611

BLAST of Spg013144 vs. TAIR 10
Match: AT4G24290.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 196.8 bits (499), Expect = 4.8e-50
Identity = 125/335 (37.31%), Postives = 189/335 (56.42%), Query Frame = 0

Query: 14  AVQALGRGFDVNFDTRLLYCKGVAGST----VVEVDDKHTRDLRLYDDVVLLNVSRDIKN 73
           A+ ++G G+D+  D RL YCKG +  +    + E DD    ++ L   + + NVS+ IK 
Sbjct: 14  AIGSIGCGYDLAIDLRLKYCKGGSKDSRLLDIKEGDD--NCEIVLPGGISIPNVSKSIK- 73

Query: 74  FQDQEPVGHRSSGICSFDEMVDYFNQRDGLSEGYSSGSFNSAFGFTGSKQTDAVATKSLS 133
             D+       S I  F +M + FNQ   L+    SG FN+ F F+   Q DA  TK+L+
Sbjct: 74  -CDKGERMRFRSDILPFQQMAEQFNQELSLAGKIPSGLFNAMFEFSSCWQKDAAYTKNLA 133

Query: 134 TDGFYIPLAKFQLTNTPFVLQENVKRAVPTSWDPPSLASFIENFGTHVITSITIGGKDVI 193
            DG +I L    L  +  +L+E+VK+AVP++WDP +LA FI+ +GTH+I S+ +GGKDVI
Sbjct: 134 FDGVFISLYSVALDKSQVLLREHVKQAVPSTWDPAALARFIDIYGTHIIVSVKMGGKDVI 193

Query: 194 YVKQHQSSPLSTMEVKRYVQDIANQRF--------SGTQSQTGSGSIKLKEKGLDGGLFN 253
           Y KQ  SS L   ++++ ++++A++RF        +G++    S  ++ KE+ L     +
Sbjct: 194 YAKQQHSSKLQPEDLQKRLKEVADKRFVEASVVHNTGSERVQASSKVETKEQRLRFADTS 253

Query: 254 NQGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQ-NHVRWARTVRSSPDVIEMTFYPITA 313
           + G Y           KED   + +RRGG+D     H  W +TV+  PDVI M+F PIT+
Sbjct: 254 SLGSY---------ANKEDYVFMCKRRGGNDNRNLMHNEWLQTVQMEPDVISMSFIPITS 313

Query: 314 LLDGVAGKEHLTRAILLYLEYKP--PLEELRYFLE 334
           LL+GV G   L+ AI LYL  KP   L  + +FLE
Sbjct: 314 LLNGVPGSGFLSHAINLYLRCKPINHLRFVHFFLE 335