Spg013066 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg013066
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	glutathione S-transferase U8-like
Location	scaffold1: 14738796 .. 14739537 (+)
RNA-Seq Expression	Spg013066
Synteny	Spg013066

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCAGCTGAACAAGTTCAGGTCTTTGGTGTCCGGTCAGGACCCTTCAGTCTGAGAGTGGAGTTGGCTCTCAGGCTCAAAGGCGTGGCTTATGAACACATTGAGGAAGATTTGCTTAACAAGAAAAGCGATTTGCTTCTGAAATACAACCCCATCTACAAGAAGGTGCCTGTCCTCGTCCACCATGGCAAACCCATTTCAGAGTCTCTCGTCATCCTCGAATACATCGAAGATAACTAGAGAGACAACCCCATCTTCCCCCAACATCCCCATGAGAAAGCTCTTGCGAGGTTTTGGGCTAAGTACATCGATGACAAGGTACGCAAAAATATGACGGGCAAAACTTCCAAGTTTTGAACTTCTTTTCAATTTAACAAGTGTTTTTGGTTTTCAGGTTTTGCCTGCGGTACAGAAAGCTGCTCGAAGCAAAGAAGGAGAGAGAGAGAAGGCTGTAGAAAAAGCGCAGGAGACGTTGGAACCACTCGAGAAAGAGCTTGAAAACAACAAGTTCTTCGGAGGAGAGAGAATTGGAATTGTGGACATTGTCGGCCTGGTTTTAGCCCATTGGATTCCTGCCATTGAAGAAGCTGTAGGGTTTGAGCTGCTAACTAGCCAGAAGTTTCTAAACCTAAGAAAATGGAGTGAAGAGTTTGTGAACCACAGCGTCGCGAAGGAAGTTTTGCCTGAAAAAGATGCGCTTGTTGGCTTTCTGAAAGATGTTACGTTCAATCAAAAAGACTAG

mRNA sequence

ATGGCAGCTGAACAAGTTCAGGTCTTTGGTGTCCGGTCAGGACCCTTCAGTCTGAGAGTGGAGTTGGCTCTCAGGCTCAAAGGCGTGGCTTATGAACACATTGAGGAAGATTTGCTTAACAAGAAAAGCGATTTGCTTCTGAAATACAACCCCATCTACAAGAAGGTGCCTGTCCTCGTTTTGCCTGCGGTACAGAAAGCTGCTCGAAGCAAAGAAGGAGAGAGAGAGAAGGCTGTAGAAAAAGCGCAGGAGACGTTGGAACCACTCGAGAAAGAGCTTGAAAACAACAAGTTCTTCGGAGGAGAGAGAATTGGAATTGTGGACATTGTCGGCCTGGTTTTAGCCCATTGGATTCCTGCCATTGAAGAAGCTGTAGGGTTTGAGCTGCTAACTAGCCAGAAGTTTCTAAACCTAAGAAAATGGAGTGAAGAGTTTGTGAACCACAGCGTCGCGAAGGAAGTTTTGCCTGAAAAAGATGCGCTTGTTGGCTTTCTGAAAGATGTTACGTTCAATCAAAAAGACTAG

Coding sequence (CDS)

ATGGCAGCTGAACAAGTTCAGGTCTTTGGTGTCCGGTCAGGACCCTTCAGTCTGAGAGTGGAGTTGGCTCTCAGGCTCAAAGGCGTGGCTTATGAACACATTGAGGAAGATTTGCTTAACAAGAAAAGCGATTTGCTTCTGAAATACAACCCCATCTACAAGAAGGTGCCTGTCCTCGTTTTGCCTGCGGTACAGAAAGCTGCTCGAAGCAAAGAAGGAGAGAGAGAGAAGGCTGTAGAAAAAGCGCAGGAGACGTTGGAACCACTCGAGAAAGAGCTTGAAAACAACAAGTTCTTCGGAGGAGAGAGAATTGGAATTGTGGACATTGTCGGCCTGGTTTTAGCCCATTGGATTCCTGCCATTGAAGAAGCTGTAGGGTTTGAGCTGCTAACTAGCCAGAAGTTTCTAAACCTAAGAAAATGGAGTGAAGAGTTTGTGAACCACAGCGTCGCGAAGGAAGTTTTGCCTGAAAAAGATGCGCTTGTTGGCTTTCTGAAAGATGTTACGTTCAATCAAAAAGACTAG

Protein sequence

MAAEQVQVFGVRSGPFSLRVELALRLKGVAYEHIEEDLLNKKSDLLLKYNPIYKKVPVLVLPAVQKAARSKEGEREKAVEKAQETLEPLEKELENNKFFGGERIGIVDIVGLVLAHWIPAIEEAVGFELLTSQKFLNLRKWSEEFVNHSVAKEVLPEKDALVGFLKDVTFNQKD

Homology

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BLAST of Spg013066 vs. NCBI nr
Match: XP_023517376.1 (probable glutathione S-transferase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 245.7 bits (626), Expect = 2.9e-61
Identity = 132/221 (59.73%), Postives = 154/221 (69.68%), Query Frame = 0


Query: 1   MAAEQVQVFGVRSGPFSLRVELALRLKGVAYEHIEEDLLNKKSDLLLKYNPIYKKVPVL- 60
           MA E+VQVFG RSGPFSLRVELAL+LK V Y+HIEEDLLN KSDLLLKYNPIYKKVPVL 
Sbjct: 1   MAEEEVQVFGFRSGPFSLRVELALKLKAVDYQHIEEDLLNNKSDLLLKYNPIYKKVPVLV 60

Query: 61  ----------------------------------------------VLPAVQKAARSKEG 120
                                                         V+PAV K +RS E 
Sbjct: 61  HNGNPISESLVILEYIEHNWTHNPIFPAHPYDRALARFWAKYIDDTVVPAVHKISRSTEE 120

Query: 121 EREKAVEKAQETLEPLEKELENNKFFGGERIGIVDIVGLVLAHWIPAIEEAVGFELLTSQ 175
           EREKA+E+AQ+TLEPLE ELEN KFFGGE IG+VDIVGL+LA WIPAIEEA+GFELL + 
Sbjct: 121 EREKAIEEAQKTLEPLENELENKKFFGGEEIGLVDIVGLILAGWIPAIEEALGFELLITH 180

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BLAST of Spg013066 vs. NCBI nr
Match: XP_038880880.1 (glutathione S-transferase U8-like [Benincasa hispida])

HSP 1 Score: 245.0 bits (624), Expect = 4.9e-61
Identity = 136/218 (62.39%), Postives = 153/218 (70.18%), Query Frame = 0


Query: 1   MAAEQVQVFGVRSGPFSLRVELALRLKGVAYEHIEEDLLNKKSDLLLKYNPIYKKVPVLV 60
           M  E+VQVFG RSGPFSLRVELAL+LKGV Y+HI+EDLLNKKSDLLLKYNPIYKKVPVLV
Sbjct: 1   MGEEEVQVFGFRSGPFSLRVELALKLKGVHYQHIDEDLLNKKSDLLLKYNPIYKKVPVLV 60

Query: 61  ------------------------------------------------LPAVQKAARSKE 120
                                                           +PAVQKAARSKE
Sbjct: 61  HHGKPISESLLILEYIEETWTENYAILPQHPHQRALARFWAKYIDDKIVPAVQKAARSKE 120

Query: 121 GEREKAVEKAQETLEPLEKELENNKFFGGERIGIVDIVGLVLAHWIPAIEEAVGFELLTS 171
            EREK++E+A E LEPLEKEL+ NKFFGG++IG VDIVGL+LA WIPA EEAVGFELL+ 
Sbjct: 121 EEREKSIEEAHEALEPLEKELK-NKFFGGDKIGFVDIVGLILACWIPATEEAVGFELLSP 180

[Close]

BLAST of Spg013066 vs. NCBI nr
Match: KAG6595362.1 (putative glutathione S-transferase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 244.6 bits (623), Expect = 6.4e-61
Identity = 133/221 (60.18%), Postives = 153/221 (69.23%), Query Frame = 0


Query: 1   MAAEQVQVFGVRSGPFSLRVELALRLKGVAYEHIEEDLLNKKSDLLLKYNPIYKKVPVL- 60
           MA E+VQVFG RSGPFSLRVELAL+LK V Y+HIEEDLLN KSDLLLKYNPIYKKVPVL 
Sbjct: 1   MAEEEVQVFGFRSGPFSLRVELALKLKAVDYQHIEEDLLNNKSDLLLKYNPIYKKVPVLV 60

Query: 61  ----------------------------------------------VLPAVQKAARSKEG 120
                                                         V+PAV K + S E 
Sbjct: 61  HNGNPISESLVILEYIEHNWTHNPIFPAHPYDRALARFWAKYIDDTVVPAVHKISGSMEK 120

Query: 121 EREKAVEKAQETLEPLEKELENNKFFGGERIGIVDIVGLVLAHWIPAIEEAVGFELLTSQ 175
           EREKA+E+AQ+TLEPLE ELEN KFFGGE IG+VDIVGL+LA WIPAIEEA+GFELL + 
Sbjct: 121 EREKAIEEAQKTLEPLENELENKKFFGGEEIGLVDIVGLILAGWIPAIEEALGFELLIAH 180

[Close]

BLAST of Spg013066 vs. NCBI nr
Match: XP_022931952.1 (probable glutathione S-transferase [Cucurbita moschata] >XP_022931953.1 probable glutathione S-transferase [Cucurbita moschata])

HSP 1 Score: 242.7 bits (618), Expect = 2.4e-60
Identity = 131/221 (59.28%), Postives = 153/221 (69.23%), Query Frame = 0


Query: 1   MAAEQVQVFGVRSGPFSLRVELALRLKGVAYEHIEEDLLNKKSDLLLKYNPIYKKVPVL- 60
           MA E+VQVFG RSGPFSLRVELAL+LK V Y+HIEEDLLN KSDLLLKYNPIYKKVPVL 
Sbjct: 1   MAEEEVQVFGFRSGPFSLRVELALKLKAVDYQHIEEDLLNNKSDLLLKYNPIYKKVPVLV 60

Query: 61  ----------------------------------------------VLPAVQKAARSKEG 120
                                                         V+PAV K +RS E 
Sbjct: 61  HNGNPISESLVILEYIEHNWTHNPIFPAHPYDRALARFWAKYIDDMVVPAVHKISRSMEK 120

Query: 121 EREKAVEKAQETLEPLEKELENNKFFGGERIGIVDIVGLVLAHWIPAIEEAVGFELLTSQ 175
           EREKA+E+AQ+TLEPLE ELE+ KFFGGE IG+VDIVGL+LA WIPAIEEA+GFELL + 
Sbjct: 121 EREKAIEEAQKTLEPLENELESKKFFGGEEIGLVDIVGLILAGWIPAIEEALGFELLITH 180

[Close]

BLAST of Spg013066 vs. NCBI nr
Match: XP_004147913.1 (glutathione S-transferase U8 [Cucumis sativus] >KGN54318.1 hypothetical protein Csa_017910 [Cucumis sativus])

HSP 1 Score: 242.7 bits (618), Expect = 2.4e-60
Identity = 130/219 (59.36%), Postives = 151/219 (68.95%), Query Frame = 0


Query: 4   EQVQVFGVRSGPFSLRVELALRLKGVAYEHIEEDLLNKKSDLLLKYNPIYKKVPVL---- 63
           ++VQVFG RSGP+SLRVELAL+LKGV YEHI+EDL+NKKSDLL+KYNPIYKKVPVL    
Sbjct: 6   QEVQVFGFRSGPYSLRVELALKLKGVVYEHIDEDLVNKKSDLLVKYNPIYKKVPVLVHHG 65

Query: 64  --------------------------------------------VLPAVQKAARSKEGER 123
                                                       V+PA QK ARS+E ER
Sbjct: 66  KPISESLVILEYIDETWTENYAILPQDPYQRALARFWAKYIDDKVVPAAQKVARSQEDER 125

Query: 124 EKAVEKAQETLEPLEKELENNKFFGGERIGIVDIVGLVLAHWIPAIEEAVGFELLTSQKF 175
           EK++E AQ TLEPLEKELEN  FFGG++IG VDIVGLVLA W+PA EEAVGFELL++ KF
Sbjct: 126 EKSIEDAQATLEPLEKELENKSFFGGDKIGFVDIVGLVLARWVPATEEAVGFELLSAHKF 185

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BLAST of Spg013066 vs. ExPASy Swiss-Prot
Match: Q9SR36 (Glutathione S-transferase U8 OS=Arabidopsis thaliana OX=3702 GN=GSTU8 PE=2 SV=1)

HSP 1 Score: 144.1 bits (362), Expect = 1.5e-33
Identity = 84/211 (39.81%), Postives = 116/211 (54.98%), Query Frame = 0


Query: 4   EQVQVFGVRSGPFSLRVELALRLKGVAYEHIEEDLLNKKSDLLLKYNPIYKKVPVL---- 63
           E V++ G+   PFS RVE+ L+LKG+ YE+IEED+   +S +LLKYNPI+KKVPVL    
Sbjct: 5   EHVKLLGLWGSPFSKRVEMVLKLKGIPYEYIEEDVYGNRSPMLLKYNPIHKKVPVLIHNG 64

Query: 64  --------------------------------------------VLPAVQKAARSKEGER 123
                                                       V+ AV+KA    E ER
Sbjct: 65  RSIAESLVIVEYIEDTWKTTHTILPQDPYERAMARFWAKYVDEKVMLAVKKACWGPESER 124

Query: 124 EKAVEKAQETLEPLEKELENNKFFGGERIGIVDIVGLVLAHWIPAIEEAVGFELLTSQKF 167
           EK V++A E L+ LEKEL +  FFGGE IG VDI    + +W+   +EA G  ++T+++F
Sbjct: 125 EKEVKEAYEGLKCLEKELGDKLFFGGETIGFVDIAADFIGYWLGIFQEASGVTIMTAEEF 184

[Close]

BLAST of Spg013066 vs. ExPASy Swiss-Prot
Match: P32110 (Probable glutathione S-transferase OS=Glycine max OX=3847 GN=HSP26-A PE=2 SV=1)

HSP 1 Score: 142.9 bits (359), Expect = 3.4e-33
Identity = 88/211 (41.71%), Postives = 115/211 (54.50%), Query Frame = 0


Query: 4   EQVQVFGVRSGPFSLRVELALRLKGVAYEHIEEDLLNKKSDLLLKYNPIYKKVPVLV--- 63
           E V++ G+   PF  RV++AL+LKGV Y+ +EE+L N KSDLLLKYNP++KKVPV V   
Sbjct: 6   EDVKLLGIVGSPFVCRVQIALKLKGVEYKFLEENLGN-KSDLLLKYNPVHKKVPVFVHNE 65

Query: 64  ----------------------LP----------------------AVQKAARS-KEGER 123
                                 LP                      AV K+  +  E ER
Sbjct: 66  QPIAESLVIVEYIDETWKNNPILPSDPYQRALARFWSKFIDDKIVGAVSKSVFTVDEKER 125

Query: 124 EKAVEKAQETLEPLEKELENNKFFGGERIGIVDIVGLVLAHWIPAIEEAVGFELLTSQKF 167
           EK VE+  E L+ LE EL++ KFFGGE  G+VDI  + +A WIP  +E  G +L TS+KF
Sbjct: 126 EKNVEETYEALQFLENELKDKKFFGGEEFGLVDIAAVFIAFWIPIFQEIAGLQLFTSEKF 185

[Close]

BLAST of Spg013066 vs. ExPASy Swiss-Prot
Match: Q03662 (Probable glutathione S-transferase OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 121.7 bits (304), Expect = 8.2e-27
Identity = 79/208 (37.98%), Postives = 107/208 (51.44%), Query Frame = 0


Query: 5   QVQVFGVRSGPFSLRVELALRLKGVAYEHIEEDLLNKKSDLLLKYNPIYKKVPVLV---- 64
           +V++ G    PFS RVE AL++KGV YE+IEED  N KS LLL+ NPI+KKVPVL+    
Sbjct: 3   EVKLLGFWYSPFSRRVEWALKIKGVKYEYIEEDRDN-KSSLLLQSNPIHKKVPVLIHNGK 62

Query: 65  ------------------------------------------LPAVQKAARSKEGEREKA 124
                                                     +PAV K    K  E+EK 
Sbjct: 63  RIVESMVILEYIDETFEGPSILPKDPYDRALARFWAKFLDDKVPAVVKTFLRKGEEQEKD 122

Query: 125 VEKAQETLEPLEKELENNKFFGGERIGIVDIVGLVLAHWIPAIEEAVGFELLTSQKFLNL 167
            E+  E L+ L+ EL++ KFF G++ G  DI   ++A W+   EEA G  L+TS+KF N 
Sbjct: 123 KEEVCEMLKVLDNELKDKKFFVGDKFGFADIAANLVAFWLGVFEEASGVVLVTSEKFPNF 182

[Close]

BLAST of Spg013066 vs. ExPASy Swiss-Prot
Match: Q03664 (Probable glutathione S-transferase OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 116.3 bits (290), Expect = 3.4e-25
Identity = 73/208 (35.10%), Postives = 104/208 (50.00%), Query Frame = 0


Query: 5   QVQVFGVRSGPFSLRVELALRLKGVAYEHIEEDLLNKKSDLLLKYNPIYKKVPVLV---- 64
           +V++ G    PF+ RVE AL+LKGV YE+IEED  N KS LLL+ NP++KKVPVL+    
Sbjct: 3   EVKLLGFWYSPFTHRVEWALKLKGVKYEYIEEDRDN-KSSLLLQSNPVHKKVPVLIHNGK 62

Query: 65  ------------------------------------------LPAVQKAARSKEGEREKA 124
                                                     + AV      K  E+EK 
Sbjct: 63  PIVESMVILEYIDETFEGPSILPKDPYDRALARFWSKFLGDKVAAVVNTFFRKGEEQEKG 122

Query: 125 VEKAQETLEPLEKELENNKFFGGERIGIVDIVGLVLAHWIPAIEEAVGFELLTSQKFLNL 167
            E+  E L+ L+ EL++ KFF G++ G  DI   ++  W+   EE  G  L+TS+KF N 
Sbjct: 123 KEEVYEMLKVLDNELKDKKFFVGDKFGFADIAANLVGFWLGVFEEGYGVVLVTSEKFPNF 182

[Close]

BLAST of Spg013066 vs. ExPASy Swiss-Prot
Match: Q03663 (Probable glutathione S-transferase OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 115.5 bits (288), Expect = 5.8e-25
Identity = 73/208 (35.10%), Postives = 102/208 (49.04%), Query Frame = 0


Query: 5   QVQVFGVRSGPFSLRVELALRLKGVAYEHIEEDLLNKKSDLLLKYNPIYKKVPVLV---- 64
           +V++ G    PFS RVE AL++KGV YE+IEED  N KS LLL+ NP+YKKVPVL+    
Sbjct: 3   EVKLLGFWYSPFSHRVEWALKIKGVKYEYIEEDRDN-KSSLLLQSNPVYKKVPVLIHNGK 62

Query: 65  ------------------------------------------LPAVQKAARSKEGEREKA 124
                                                     + AV      K  E+EK 
Sbjct: 63  PIVESMIILEYIDETFEGPSILPKDPYDRALARFWAKFLDDKVAAVVNTFFRKGEEQEKG 122

Query: 125 VEKAQETLEPLEKELENNKFFGGERIGIVDIVGLVLAHWIPAIEEAVGFELLTSQKFLNL 167
            E+  E L+ L+ EL++ KFF G++ G  DI   ++  W+   EE  G  L+ S+KF N 
Sbjct: 123 KEEVYEMLKVLDNELKDKKFFAGDKFGFADIAANLVGFWLGVFEEGYGDVLVKSEKFPNF 182

[Close]

BLAST of Spg013066 vs. ExPASy TrEMBL
Match: A0A6J1F079 (probable glutathione S-transferase OS=Cucurbita moschata OX=3662 GN=LOC111438219 PE=3 SV=1)

HSP 1 Score: 242.7 bits (618), Expect = 1.2e-60
Identity = 131/221 (59.28%), Postives = 153/221 (69.23%), Query Frame = 0


Query: 1   MAAEQVQVFGVRSGPFSLRVELALRLKGVAYEHIEEDLLNKKSDLLLKYNPIYKKVPVL- 60
           MA E+VQVFG RSGPFSLRVELAL+LK V Y+HIEEDLLN KSDLLLKYNPIYKKVPVL 
Sbjct: 1   MAEEEVQVFGFRSGPFSLRVELALKLKAVDYQHIEEDLLNNKSDLLLKYNPIYKKVPVLV 60

Query: 61  ----------------------------------------------VLPAVQKAARSKEG 120
                                                         V+PAV K +RS E 
Sbjct: 61  HNGNPISESLVILEYIEHNWTHNPIFPAHPYDRALARFWAKYIDDMVVPAVHKISRSMEK 120

Query: 121 EREKAVEKAQETLEPLEKELENNKFFGGERIGIVDIVGLVLAHWIPAIEEAVGFELLTSQ 175
           EREKA+E+AQ+TLEPLE ELE+ KFFGGE IG+VDIVGL+LA WIPAIEEA+GFELL + 
Sbjct: 121 EREKAIEEAQKTLEPLENELESKKFFGGEEIGLVDIVGLILAGWIPAIEEALGFELLITH 180

[Close]

BLAST of Spg013066 vs. ExPASy TrEMBL
Match: A0A0A0KXR4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G303170 PE=3 SV=1)

HSP 1 Score: 242.7 bits (618), Expect = 1.2e-60
Identity = 130/219 (59.36%), Postives = 151/219 (68.95%), Query Frame = 0


Query: 4   EQVQVFGVRSGPFSLRVELALRLKGVAYEHIEEDLLNKKSDLLLKYNPIYKKVPVL---- 63
           ++VQVFG RSGP+SLRVELAL+LKGV YEHI+EDL+NKKSDLL+KYNPIYKKVPVL    
Sbjct: 6   QEVQVFGFRSGPYSLRVELALKLKGVVYEHIDEDLVNKKSDLLVKYNPIYKKVPVLVHHG 65

Query: 64  --------------------------------------------VLPAVQKAARSKEGER 123
                                                       V+PA QK ARS+E ER
Sbjct: 66  KPISESLVILEYIDETWTENYAILPQDPYQRALARFWAKYIDDKVVPAAQKVARSQEDER 125

Query: 124 EKAVEKAQETLEPLEKELENNKFFGGERIGIVDIVGLVLAHWIPAIEEAVGFELLTSQKF 175
           EK++E AQ TLEPLEKELEN  FFGG++IG VDIVGLVLA W+PA EEAVGFELL++ KF
Sbjct: 126 EKSIEDAQATLEPLEKELENKSFFGGDKIGFVDIVGLVLARWVPATEEAVGFELLSAHKF 185

[Close]

BLAST of Spg013066 vs. ExPASy TrEMBL
Match: A0A6J1HSP0 (probable glutathione S-transferase OS=Cucurbita maxima OX=3661 GN=LOC111466262 PE=3 SV=1)

HSP 1 Score: 240.0 bits (611), Expect = 7.6e-60
Identity = 131/221 (59.28%), Postives = 151/221 (68.33%), Query Frame = 0


Query: 1   MAAEQVQVFGVRSGPFSLRVELALRLKGVAYEHIEEDLLNKKSDLLLKYNPIYKKVPVL- 60
           MA E+VQVFG RSGPFSLRVELAL+LK V Y+HIEEDLLN KSDLLLKYNPIYKKVPVL 
Sbjct: 1   MAEEEVQVFGFRSGPFSLRVELALKLKAVDYQHIEEDLLNNKSDLLLKYNPIYKKVPVLV 60

Query: 61  ----------------------------------------------VLPAVQKAARSKEG 120
                                                         V+PAV K + S E 
Sbjct: 61  HNGNPISESLVILEYIEHNWTHNPIFPAHPYDRALARFWAKFIDDTVVPAVHKISGSTEE 120

Query: 121 EREKAVEKAQETLEPLEKELENNKFFGGERIGIVDIVGLVLAHWIPAIEEAVGFELLTSQ 175
           EREKA+E+AQ+TLEPLE EL N KFFGGE IG+VDIVGL+LA WIPAIEEA+GFELL + 
Sbjct: 121 EREKAIEEAQKTLEPLENELGNKKFFGGEEIGLVDIVGLILAGWIPAIEEALGFELLNTH 180

[Close]

BLAST of Spg013066 vs. ExPASy TrEMBL
Match: A0A5D3DJ09 (Glutathione S-transferase U8-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold500G001410 PE=3 SV=1)

HSP 1 Score: 235.3 bits (599), Expect = 1.9e-58
Identity = 127/219 (57.99%), Postives = 149/219 (68.04%), Query Frame = 0


Query: 4   EQVQVFGVRSGPFSLRVELALRLKGVAYEHIEEDLLNKKSDLLLKYNPIYKKVPVL---- 63
           ++VQVFG R GP+SLRVELAL+LKGV Y+HI+EDLLNKKSDLL+KYNP+YKKVPVL    
Sbjct: 6   QEVQVFGFRLGPYSLRVELALKLKGVVYKHIDEDLLNKKSDLLVKYNPVYKKVPVLVHHG 65

Query: 64  --------------------------------------------VLPAVQKAARSKEGER 123
                                                       V+PAVQK ARS+E ER
Sbjct: 66  KPISESLVILEYIEETWTQNYTILPKDPYQRALARFWAKYIDEKVVPAVQKVARSQEDER 125

Query: 124 EKAVEKAQETLEPLEKELENNKFFGGERIGIVDIVGLVLAHWIPAIEEAVGFELLTSQKF 175
           EK++E AQ  LEPLE+EL+N  FFGG+ IG VDI GLVLA WIPA EEAVGFELL++ KF
Sbjct: 126 EKSIEDAQAALEPLEEELKNKLFFGGDTIGFVDIAGLVLARWIPATEEAVGFELLSAHKF 185

[Close]

BLAST of Spg013066 vs. ExPASy TrEMBL
Match: A0A1S3BK49 (glutathione S-transferase U8-like OS=Cucumis melo OX=3656 GN=LOC103490744 PE=3 SV=1)

HSP 1 Score: 235.3 bits (599), Expect = 1.9e-58
Identity = 127/219 (57.99%), Postives = 149/219 (68.04%), Query Frame = 0


Query: 4   EQVQVFGVRSGPFSLRVELALRLKGVAYEHIEEDLLNKKSDLLLKYNPIYKKVPVL---- 63
           ++VQVFG R GP+SLRVELAL+LKGV Y+HI+EDLLNKKSDLL+KYNP+YKKVPVL    
Sbjct: 6   QEVQVFGFRLGPYSLRVELALKLKGVVYKHIDEDLLNKKSDLLVKYNPVYKKVPVLVHHG 65

Query: 64  --------------------------------------------VLPAVQKAARSKEGER 123
                                                       V+PAVQK ARS+E ER
Sbjct: 66  KPISESLVILEYIEETWTQNYTILPKDPYQRALARFWAKYIDEKVVPAVQKVARSQEDER 125

Query: 124 EKAVEKAQETLEPLEKELENNKFFGGERIGIVDIVGLVLAHWIPAIEEAVGFELLTSQKF 175
           EK++E AQ  LEPLE+EL+N  FFGG+ IG VDI GLVLA WIPA EEAVGFELL++ KF
Sbjct: 126 EKSIEDAQAALEPLEEELKNKLFFGGDTIGFVDIAGLVLARWIPATEEAVGFELLSAHKF 185

[Close]

BLAST of Spg013066 vs. TAIR 10
Match: AT3G09270.1 (glutathione S-transferase TAU 8 )

HSP 1 Score: 144.1 bits (362), Expect = 1.1e-34
Identity = 84/211 (39.81%), Postives = 116/211 (54.98%), Query Frame = 0


Query: 4   EQVQVFGVRSGPFSLRVELALRLKGVAYEHIEEDLLNKKSDLLLKYNPIYKKVPVL---- 63
           E V++ G+   PFS RVE+ L+LKG+ YE+IEED+   +S +LLKYNPI+KKVPVL    
Sbjct: 5   EHVKLLGLWGSPFSKRVEMVLKLKGIPYEYIEEDVYGNRSPMLLKYNPIHKKVPVLIHNG 64

Query: 64  --------------------------------------------VLPAVQKAARSKEGER 123
                                                       V+ AV+KA    E ER
Sbjct: 65  RSIAESLVIVEYIEDTWKTTHTILPQDPYERAMARFWAKYVDEKVMLAVKKACWGPESER 124

Query: 124 EKAVEKAQETLEPLEKELENNKFFGGERIGIVDIVGLVLAHWIPAIEEAVGFELLTSQKF 167
           EK V++A E L+ LEKEL +  FFGGE IG VDI    + +W+   +EA G  ++T+++F
Sbjct: 125 EKEVKEAYEGLKCLEKELGDKLFFGGETIGFVDIAADFIGYWLGIFQEASGVTIMTAEEF 184

[Close]

BLAST of Spg013066 vs. TAIR 10
Match: AT2G29420.1 (glutathione S-transferase tau 7 )

HSP 1 Score: 104.8 bits (260), Expect = 7.3e-23
Identity = 65/211 (30.81%), Postives = 105/211 (49.76%), Query Frame = 0


Query: 3   AEQVQVFGVRSGPFSLRVELALRLKGVAYEHIEEDLLNKKSDLLLKYNPIYKKVPVLV-- 62
           +E+V++ G+ + PFS R+E+AL LKGV+YE +E+D+ N KS LLL+ NP++K +PVLV  
Sbjct: 7   SEEVKLLGMWASPFSRRIEIALTLKGVSYEFLEQDITN-KSSLLLQLNPVHKMIPVLVHN 66

Query: 63  -----------------------LP----------------------AVQKAARSKEGER 122
                                  LP                         K       ER
Sbjct: 67  GKPISESLVILEYIDETWRDNPILPQDPYERTMARFWSKFVDEQIYVTAMKVVGKTGKER 126

Query: 123 EKAVEKAQETLEPLEKELENNKFFGGERIGIVDIVGLVLAHWIPAIEEAVGFELLTSQKF 167
           +  VE  ++ L  LEKEL    F GG+ +G VDIV  ++A W+   EE VG +++  +KF
Sbjct: 127 DAVVEATRDLLMFLEKELVGKDFLGGKSLGFVDIVATLVAFWLMRTEEIVGVKVVPVEKF 186

[Close]

BLAST of Spg013066 vs. TAIR 10
Match: AT2G29460.1 (glutathione S-transferase tau 4 )

HSP 1 Score: 97.1 bits (240), Expect = 1.5e-20
Identity = 66/214 (30.84%), Postives = 107/214 (50.00%), Query Frame = 0


Query: 4   EQVQVFGVRSGPFSLRVELALRLKGVAYEHIEEDLLNKKSDLLLKYNPIYKKVPVLV--- 63
           E V++ G  + PF+ RVE+A +LKGV YE++E+D++N KS LLL+ NP+YKKVPVLV   
Sbjct: 6   EDVKLLGFWASPFTRRVEMAFKLKGVPYEYLEQDIVN-KSPLLLQINPVYKKVPVLVYKG 65

Query: 64  ----------------------LP-----------------------AVQKAARSKEGER 123
                                 LP                       A    A++++G  
Sbjct: 66  KILSESHVILEYIDQIWKNNPILPQDPYEKAMALFWAKFVDEQVGPVAFMSVAKAEKGV- 125

Query: 124 EKAVEKAQETLEPLEKELENNKFFGGERIGIVDIV-GLVLAHWIPAIEEAVGFELLTSQK 169
           E A+++AQE    LEKE+    FFGG+ IG +D+V G ++   +    E +G +++  +K
Sbjct: 126 EVAIKEAQELFMFLEKEVTGKDFFGGKTIGFLDLVAGSMIPFCLARGWEGMGIDMIPEEK 185

[Close]

BLAST of Spg013066 vs. TAIR 10
Match: AT2G29450.1 (glutathione S-transferase tau 5 )

HSP 1 Score: 93.6 bits (231), Expect = 1.7e-19
Identity = 64/205 (31.22%), Postives = 98/205 (47.80%), Query Frame = 0


Query: 4   EQVQVFGVRSGPFSLRVELALRLKGVAYEHIEEDLLNKKSDLLLKYNPIYKKVPVLV--- 63
           E+V++ G+ + PFS RVE+AL+LKG+ YE++EE +L  KS LLL  NPI+KKVPVLV   
Sbjct: 5   EEVKLLGIWASPFSRRVEMALKLKGIPYEYVEE-ILENKSPLLLALNPIHKKVPVLVHNG 64

Query: 64  ----------------------LP-----------------------AVQKAARSKEGER 123
                                 LP                            AR+ E  R
Sbjct: 65  KTILESHVILEYIDETWPQNPILPQDPYERSKARFFAKLVDEQIMNVGFISMARADEKGR 124

Query: 124 EKAVEKAQETLEPLEKELENNKFFGGERIGIVDIV-GLVLAHWIPAIEEAVGFELLTSQK 160
           E   E+ +E +  LEKEL    +FGG+ +G +D V G ++   +    E +G E++T +K
Sbjct: 125 EVLAEQVRELIMYLEKELVGKDYFGGKTVGFLDFVAGSLIPFCLERGWEGIGLEVITEEK 184

[Close]

BLAST of Spg013066 vs. TAIR 10
Match: AT1G59700.1 (glutathione S-transferase TAU 16 )

HSP 1 Score: 92.0 bits (227), Expect = 4.9e-19
Identity = 67/219 (30.59%), Postives = 105/219 (47.95%), Query Frame = 0


Query: 4   EQVQVFGVRSGPFSLRVELALRLKGVAYEHIEEDLLNKKSDLLLKYNPIYKKVPVLV--- 63
           E+V++ GV   P+++R ++ALRLK V Y+++EE+L   KS+LLLK NP++KKVPVL+   
Sbjct: 5   EEVKLLGVWYSPYAIRPKIALRLKSVDYDYVEENLFGSKSELLLKSNPVHKKVPVLLHNN 64

Query: 64  ----------------------------------------------LPAVQKAARSK-EG 123
                                                          PA++ AA +K E 
Sbjct: 65  KPIVESLNIVEYIDETWNSSAPSILPSHPYDRALARFWSDFVDNKWFPALRMAAITKSED 124

Query: 124 EREKAVEKAQETLEPLEKEL----ENNKFFGGERIGIVDIVGLVLAHWIPAIEEAVGFEL 169
            + KA+E+ +E L  LE       +   FFGGE IG +DI        + A E+    +L
Sbjct: 125 AKAKAMEEVEEGLLQLEDAFVSISKGKPFFGGEAIGFMDICFGSFVVLLKAREKFKAEKL 184

The following BLAST results are available for this feature:

BLAST of Spg013066 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023517376.1	2.9e-61	59.73	probable glutathione S-transferase [Cucurbita pepo subsp. pepo]	[more]
XP_038880880.1	4.9e-61	62.39	glutathione S-transferase U8-like [Benincasa hispida]	[more]
KAG6595362.1	6.4e-61	60.18	putative glutathione S-transferase, partial [Cucurbita argyrosperma subsp. soror...	[more]
XP_022931952.1	2.4e-60	59.28	probable glutathione S-transferase [Cucurbita moschata] >XP_022931953.1 probable...	[more]
XP_004147913.1	2.4e-60	59.36	glutathione S-transferase U8 [Cucumis sativus] >KGN54318.1 hypothetical protein ...	[more]

BLAST of Spg013066 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SR36	1.5e-33	39.81	Glutathione S-transferase U8 OS=Arabidopsis thaliana OX=3702 GN=GSTU8 PE=2 SV=1	[more]
P32110	3.4e-33	41.71	Probable glutathione S-transferase OS=Glycine max OX=3847 GN=HSP26-A PE=2 SV=1	[more]
Q03662	8.2e-27	37.98	Probable glutathione S-transferase OS=Nicotiana tabacum OX=4097 PE=2 SV=1	[more]
Q03664	3.4e-25	35.10	Probable glutathione S-transferase OS=Nicotiana tabacum OX=4097 PE=2 SV=1	[more]
Q03663	5.8e-25	35.10	Probable glutathione S-transferase OS=Nicotiana tabacum OX=4097 PE=2 SV=1	[more]

BLAST of Spg013066 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1F079	1.2e-60	59.28	probable glutathione S-transferase OS=Cucurbita moschata OX=3662 GN=LOC111438219...	[more]
A0A0A0KXR4	1.2e-60	59.36	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G303170 PE=3 SV=1	[more]
A0A6J1HSP0	7.6e-60	59.28	probable glutathione S-transferase OS=Cucurbita maxima OX=3661 GN=LOC111466262 P...	[more]
A0A5D3DJ09	1.9e-58	57.99	Glutathione S-transferase U8-like OS=Cucumis melo var. makuwa OX=1194695 GN=E567...	[more]
A0A1S3BK49	1.9e-58	57.99	glutathione S-transferase U8-like OS=Cucumis melo OX=3656 GN=LOC103490744 PE=3 S...	[more]

BLAST of Spg013066 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G09270.1	1.1e-34	39.81	glutathione S-transferase TAU 8	[more]
AT2G29420.1	7.3e-23	30.81	glutathione S-transferase tau 7	[more]
AT2G29460.1	1.5e-20	30.84	glutathione S-transferase tau 4	[more]
AT2G29450.1	1.7e-19	31.22	glutathione S-transferase tau 5	[more]
AT1G59700.1	4.9e-19	30.59	glutathione S-transferase TAU 16	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.40.30.10	Glutaredoxin	coord: 1..63 e-value: 6.2E-19 score: 69.8
None	No IPR available	GENE3D	3.40.30.10	Glutaredoxin	coord: 157..167 e-value: 1.4E-11 score: 46.5
None	No IPR available	GENE3D	1.20.1050.10		coord: 64..156 e-value: 1.4E-11 score: 46.5
None	No IPR available	PANTHER	PTHR11260	GLUTATHIONE S-TRANSFERASE, GST, SUPERFAMILY, GST DOMAIN CONTAINING	coord: 66..168
None	No IPR available	PANTHER	PTHR11260	GLUTATHIONE S-TRANSFERASE, GST, SUPERFAMILY, GST DOMAIN CONTAINING	coord: 4..60
None	No IPR available	PANTHER	PTHR11260:SF651	GLUTATHIONE S-TRANSFERASE, AMINO-TERMINAL DOMAIN PROTEIN	coord: 4..60
None	No IPR available	PANTHER	PTHR11260:SF651	GLUTATHIONE S-TRANSFERASE, AMINO-TERMINAL DOMAIN PROTEIN	coord: 66..168
IPR004045	Glutathione S-transferase, N-terminal	PFAM	PF02798	GST_N	coord: 5..60 e-value: 4.2E-8 score: 33.4
IPR004045	Glutathione S-transferase, N-terminal	PROSITE	PS50404	GST_NTER	coord: 4..96 score: 9.344754
IPR004046	Glutathione S-transferase, C-terminal	PFAM	PF00043	GST_C	coord: 58..147 e-value: 3.6E-11 score: 43.1
IPR010987	Glutathione S-transferase, C-terminal-like	PROSITE	PS50405	GST_CTER	coord: 21..170 score: 12.850934
IPR045074	Glutathione S-transferases Tau, C-terminal alpha helical domain, plant	CDD	cd03185	GST_C_Tau	coord: 60..167 e-value: 5.25997E-33 score: 112.272
IPR036282	Glutathione S-transferase, C-terminal domain superfamily	SUPERFAMILY	47616	GST C-terminal domain-like	coord: 61..166
IPR036249	Thioredoxin-like superfamily	SUPERFAMILY	52833	Thioredoxin-like	coord: 5..61

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg013066.1	Spg013066.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006749	glutathione metabolic process
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0004364	glutathione transferase activity
molecular_function	GO:0005515	protein binding