Spg012898 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg012898
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionF-box domain-containing protein
Locationscaffold1: 4940130 .. 4941284 (+)
RNA-Seq ExpressionSpg012898
SyntenySpg012898
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGAAATATCGGGTACGGTGGTCCGATCTCCCTCCTGAACTTTGGCCCCTCATCGGAAAACACCTCGATACTTACATCGATGTCCTCCGATTTCGCAGCGTCTGTAGATCTTGGCGCGCTTCTCTTCCTCCTTTGAACGCCATTTCTCCTCTCCACTTCCCTTATTACTCTTACTCCACCACCGCCGCCAATCGACGAATCGACGCCTTACTCTTCCGAAGGATAATCTATCACCTCACTCCCCTCCATGATGATCGTATCTCTTCTTCTTCTGCGGCGGAGGGCTGGTTTGCGATGGTGGAGAGTACAGAGCCCGGTAAAATGCGTTTTCAGGATCTGCTTTCTAATCGCTATGTCAAACTCGACAACGCCAACCGAGCCCTTCGGAACGAAGTGAACTGGCTGGACTTTCGAATCCACGAAGTAGCTCAATCGTATACGCTTGAATACAGTCATACTGTTGGTTTTCCTGTTTCTGGAGTTATCAAGGTTGTAATGTCCCCTGATTCTGTTTGGACCGATCTCAGGAACTGCACCATTGTAGCAGTTTACGATGGAGGGAAATTAGGGTTTGCTAAACACGGGGATGATAGTTGGACGCTGATCGATGACCAGAACTTTCAGTACGAAGACGTGATTGTGTACAAGGGGCAGGTTTGTGCGGTTGATATACGGGGAACAGTTTCCTGGATCGATTCGTCGATGAAATTGGAACAATCTTCTTCTGAATTGCCTGACGGTAACCCGACGCATTTGGTGGAGTCTGGCGGCAAACTTTATGCTGTTCAAGAATTGATGATTGTCAATTACTGGGAAGTAATCGATATCAAAGTCCACAGGCTGATCCAAAAGCGGGGAAGATGGGAGGAGGTGAAAAATTTGGGGGATCAAGCCTTCGTTATAGGGAAGGGCTGTTGTTTCTCAGTGTCAGCCCCAGATTTTAAAGGACTTAAAGGAAATCGCATATATTTTGCTAAAGCACGAAGATACAATGTCCGCCATAGGTGTTATGCATTCGACCTTGAGAAAAGAAGGGTTTCAGAAGCCTCTATCCATGGCAATGCACAAATATTCGAACCACCCCGAATTTTTGGACCACCCCGAATTCTTCTTCGTACTGTATACGGACATCCTTCCAGATGGTACGATTAG

mRNA sequence

ATGGAGAAATATCGGGTACGGTGGTCCGATCTCCCTCCTGAACTTTGGCCCCTCATCGGAAAACACCTCGATACTTACATCGATGTCCTCCGATTTCGCAGCGTCTGTAGATCTTGGCGCGCTTCTCTTCCTCCTTTGAACGCCATTTCTCCTCTCCACTTCCCTTATTACTCTTACTCCACCACCGCCGCCAATCGACGAATCGACGCCTTACTCTTCCGAAGGATAATCTATCACCTCACTCCCCTCCATGATGATCGTATCTCTTCTTCTTCTGCGGCGGAGGGCTGGTTTGCGATGGTGGAGAGTACAGAGCCCGGTAAAATGCGTTTTCAGGATCTGCTTTCTAATCGCTATGTCAAACTCGACAACGCCAACCGAGCCCTTCGGAACGAAGTGAACTGGCTGGACTTTCGAATCCACGAAGTAGCTCAATCGTATACGCTTGAATACAGTCATACTGTTGGTTTTCCTGTTTCTGGAGTTATCAAGGTTGTAATGTCCCCTGATTCTGTTTGGACCGATCTCAGGAACTGCACCATTGTAGCAGTTTACGATGGAGGGAAATTAGGGTTTGCTAAACACGGGGATGATAGTTGGACGCTGATCGATGACCAGAACTTTCAGTACGAAGACGTGATTGTGTACAAGGGGCAGGTTTGTGCGGTTGATATACGGGGAACAGTTTCCTGGATCGATTCGTCGATGAAATTGGAACAATCTTCTTCTGAATTGCCTGACGGTAACCCGACGCATTTGGTGGAGTCTGGCGGCAAACTTTATGCTGTTCAAGAATTGATGATTGTCAATTACTGGGAAGTAATCGATATCAAAGTCCACAGGCTGATCCAAAAGCGGGGAAGATGGGAGGAGGTGAAAAATTTGGGGGATCAAGCCTTCGTTATAGGGAAGGGCTGTTGTTTCTCAGTGTCAGCCCCAGATTTTAAAGGACTTAAAGGAAATCGCATATATTTTGCTAAAGCACGAAGATACAATGTCCGCCATAGGTGTTATGCATTCGACCTTGAGAAAAGAAGGGTTTCAGAAGCCTCTATCCATGGCAATGCACAAATATTCGAACCACCCCGAATTTTTGGACCACCCCGAATTCTTCTTCGTACTGTATACGGACATCCTTCCAGATGGTACGATTAG

Coding sequence (CDS)

ATGGAGAAATATCGGGTACGGTGGTCCGATCTCCCTCCTGAACTTTGGCCCCTCATCGGAAAACACCTCGATACTTACATCGATGTCCTCCGATTTCGCAGCGTCTGTAGATCTTGGCGCGCTTCTCTTCCTCCTTTGAACGCCATTTCTCCTCTCCACTTCCCTTATTACTCTTACTCCACCACCGCCGCCAATCGACGAATCGACGCCTTACTCTTCCGAAGGATAATCTATCACCTCACTCCCCTCCATGATGATCGTATCTCTTCTTCTTCTGCGGCGGAGGGCTGGTTTGCGATGGTGGAGAGTACAGAGCCCGGTAAAATGCGTTTTCAGGATCTGCTTTCTAATCGCTATGTCAAACTCGACAACGCCAACCGAGCCCTTCGGAACGAAGTGAACTGGCTGGACTTTCGAATCCACGAAGTAGCTCAATCGTATACGCTTGAATACAGTCATACTGTTGGTTTTCCTGTTTCTGGAGTTATCAAGGTTGTAATGTCCCCTGATTCTGTTTGGACCGATCTCAGGAACTGCACCATTGTAGCAGTTTACGATGGAGGGAAATTAGGGTTTGCTAAACACGGGGATGATAGTTGGACGCTGATCGATGACCAGAACTTTCAGTACGAAGACGTGATTGTGTACAAGGGGCAGGTTTGTGCGGTTGATATACGGGGAACAGTTTCCTGGATCGATTCGTCGATGAAATTGGAACAATCTTCTTCTGAATTGCCTGACGGTAACCCGACGCATTTGGTGGAGTCTGGCGGCAAACTTTATGCTGTTCAAGAATTGATGATTGTCAATTACTGGGAAGTAATCGATATCAAAGTCCACAGGCTGATCCAAAAGCGGGGAAGATGGGAGGAGGTGAAAAATTTGGGGGATCAAGCCTTCGTTATAGGGAAGGGCTGTTGTTTCTCAGTGTCAGCCCCAGATTTTAAAGGACTTAAAGGAAATCGCATATATTTTGCTAAAGCACGAAGATACAATGTCCGCCATAGGTGTTATGCATTCGACCTTGAGAAAAGAAGGGTTTCAGAAGCCTCTATCCATGGCAATGCACAAATATTCGAACCACCCCGAATTTTTGGACCACCCCGAATTCTTCTTCGTACTGTATACGGACATCCTTCCAGATGGTACGATTAG

Protein sequence

MEKYRVRWSDLPPELWPLIGKHLDTYIDVLRFRSVCRSWRASLPPLNAISPLHFPYYSYSTTAANRRIDALLFRRIIYHLTPLHDDRISSSSAAEGWFAMVESTEPGKMRFQDLLSNRYVKLDNANRALRNEVNWLDFRIHEVAQSYTLEYSHTVGFPVSGVIKVVMSPDSVWTDLRNCTIVAVYDGGKLGFAKHGDDSWTLIDDQNFQYEDVIVYKGQVCAVDIRGTVSWIDSSMKLEQSSSELPDGNPTHLVESGGKLYAVQELMIVNYWEVIDIKVHRLIQKRGRWEEVKNLGDQAFVIGKGCCFSVSAPDFKGLKGNRIYFAKARRYNVRHRCYAFDLEKRRVSEASIHGNAQIFEPPRIFGPPRILLRTVYGHPSRWYD
Homology
BLAST of Spg012898 vs. NCBI nr
Match: XP_038881861.1 (putative F-box protein At1g65770 [Benincasa hispida])

HSP 1 Score: 410.6 bits (1054), Expect = 1.5e-110
Identity = 228/407 (56.02%), Postives = 267/407 (65.60%), Query Frame = 0

Query: 1   MEKYRVRWSDLPPELWPLIGKHLDTYIDVLRFRSVCRSWRASLPPLNAISPLHFPYYSYS 60
           ME  RVRWSDLPPELWP+IGK LDT+ID+LRFRSVCRSWRASLPP NAISPL        
Sbjct: 1   MEDSRVRWSDLPPELWPIIGKRLDTHIDILRFRSVCRSWRASLPPFNAISPLLPLDLPSP 60

Query: 61  TTAANRRIDALLFRRIIYHLTPLH----DDRISSSS--------------AAEGWFAMVE 120
             AA+   DA L RRIIY L+PLH     D  SSSS              AAEGW A VE
Sbjct: 61  VFAADHLTDAFLIRRIIYRLSPLHHRQTSDSASSSSSSSSSSSSSSSSSCAAEGWLAKVE 120

Query: 121 STEPGKMRFQDLLSNRYVKLDNANRALRNEVNWLDFRIHEVAQSYTLEYSHTVGFPVSGV 180
           ST+ GKMRF   LS RY K +  N   R EVN LDF I+EVA+SYTL Y  T G  V  +
Sbjct: 121 STKLGKMRFLHPLSTRYAKYN--NELFRKEVNLLDFGIYEVAKSYTLGY--TNGALVPRI 180

Query: 181 IKVVMSPDSVWTDLRNCTIVAVYDGGKLGFAKHGDDSWTLIDDQNFQYEDVIVYKGQVCA 240
            KVVM PDS W D++NCTI+AVY GGKLGFAKHGDD WTLIDD+NF Y+DVIVYKGQ  A
Sbjct: 181 TKVVMFPDSPWIDVKNCTILAVYAGGKLGFAKHGDDKWTLIDDRNFHYDDVIVYKGQFYA 240

Query: 241 VDIRGTVSWIDSSMKLEQSSSELPD-GNPTHLVESGGKLYAVQELMIVN----------- 300
           VD  GTV WIDSSMKL Q S  L   GN  HLVESGG+LY V   +              
Sbjct: 241 VDRWGTVFWIDSSMKLVQFSPPLCGFGNQKHLVESGGELYVVDRFLDKEPSLWNADIFHI 300

Query: 301 YW----------EVIDIKVHRLIQKRGRWEEVKNLGDQAFVIGKGCCFSVSAPDFKGLKG 360
           +W          +VID K+HRL Q+ GRW EVK+LG+Q+FV+G  CCFSVS+P F+G+KG
Sbjct: 301 HWLNNLIEDSPPKVIDFKLHRLDQEWGRWVEVKSLGNQSFVLGNDCCFSVSSPHFEGVKG 360

Query: 361 NRIYFAKARRYNVRHRCYAFDLEKRRVSEASIH--GNAQIFEPPRIF 366
           + IYF    +  + +  Y F+LE++R+ +ASI+   NA IF PP I+
Sbjct: 361 SCIYFTHTPKCTLGYNTYVFELEEKRILKASINDIDNAPIFGPPPIW 403

BLAST of Spg012898 vs. NCBI nr
Match: XP_016899202.1 (PREDICTED: putative F-box protein At1g65770 [Cucumis melo] >KAA0025628.1 putative F-box protein [Cucumis melo var. makuwa] >TYK12503.1 putative F-box protein [Cucumis melo var. makuwa])

HSP 1 Score: 403.7 bits (1036), Expect = 1.8e-108
Identity = 221/400 (55.25%), Postives = 263/400 (65.75%), Query Frame = 0

Query: 1   MEKYRVRWSDLPPELWPLIGKHLDTYIDVLRFRSVCRSWRASLPPLNAISPLHFPYYSYS 60
           ME  RVRWSDLPPELWP+IGK LDTYIDVLRFRSVCRSWRASLPP NA+SPL        
Sbjct: 1   MEDSRVRWSDLPPELWPIIGKRLDTYIDVLRFRSVCRSWRASLPPFNAVSPLLPLDLPSP 60

Query: 61  TTAANRRIDALLFRRIIYHLTPLHDDRI-------------SSSSAAEGWFAMVESTEPG 120
             AA+   DA L RRIIY L+PL   +              SS  AAEGW A VEST+ G
Sbjct: 61  VFAADHLTDAFLIRRIIYRLSPLDHHKTFSFASSSTSSSSSSSYCAAEGWLAKVESTKLG 120

Query: 121 KMRFQDLLSNRYVKLDNANRALRNEVNWLDFRIHEVAQSYTLEYSHTVGFPVSGVIKVVM 180
           KMRF   LS RY K +  +   R EVN LDF I+EVA+SYTL Y  T G PV  + KVVM
Sbjct: 121 KMRFLHPLSTRYAKCN--SDLFRKEVNLLDFGIYEVAKSYTLGY--TNGSPVPRITKVVM 180

Query: 181 SPDSVWTDLRNCTIVAVYDGGKLGFAKHGDDSWTLIDDQNFQYEDVIVYKGQVCAVDIRG 240
            PDS W D++ CTI+AVY GGKLGFAKHGDD WTLID +NF Y+DVIVYKGQ  AVD  G
Sbjct: 181 FPDSPWIDVKKCTILAVYAGGKLGFAKHGDDKWTLIDHRNFHYDDVIVYKGQFYAVDRWG 240

Query: 241 TVSWIDSSMKLEQSSSELPD-GNPTHLVESGGKLYAV-----QELMIVN------YW--- 300
           TV WIDSSM+L Q S  L   GN  HLVE  G+LY V     +E ++ N      +W   
Sbjct: 241 TVFWIDSSMRLVQFSPPLCGFGNQKHLVECNGELYVVDRFLDKEPLLWNADIFHIHWLNN 300

Query: 301 -------EVIDIKVHRLIQKRGRWEEVKNLGDQAFVIGKGCCFSVSAPDFKGLKGNRIYF 360
                  +VID K+HRL Q+ GRW EVKNLG+Q+F++G  CCFSVS P+F+GLKG+ IYF
Sbjct: 301 LIEDSPPKVIDFKLHRLDQEWGRWVEVKNLGNQSFILGNDCCFSVSTPNFEGLKGSCIYF 360

Query: 361 AKARRYNVRHRCYAFDLEKRRVSEASIHGNAQIFEPPRIF 366
               +  + +  + F+LE +R+ +AS + NA IF PP I+
Sbjct: 361 THTPKCALGYNTHVFELEDKRILKASSNDNAPIFRPPPIW 396

BLAST of Spg012898 vs. NCBI nr
Match: XP_023518907.1 (putative F-box protein At1g65770 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 399.1 bits (1024), Expect = 4.5e-107
Identity = 220/395 (55.70%), Postives = 266/395 (67.34%), Query Frame = 0

Query: 1   MEKYRVRWSDLPPELWPLIGKHLDTYIDVLRFRSVCRSWRASLPPLNAIS---PLHFPYY 60
           M+  RV+WSDLPPE+ P+IGK L+TYIDVLRFRSVCRSWRASLPP N +S   PL  P  
Sbjct: 1   MDDSRVQWSDLPPEILPIIGKSLETYIDVLRFRSVCRSWRASLPPFNVVSSLLPLDLPSP 60

Query: 61  SYSTTAANRRIDALLFRRIIYHLTPL-----HDDRISSSSAAEGWFAMVESTEPGKMRFQ 120
            ++   A+   DALL RR+IY L+PL      D   SSS AAEGW A VEST+ G MRF 
Sbjct: 61  IFT---ADHLTDALLIRRVIYRLSPLDHQHSSDSASSSSFAAEGWLAKVESTKLGTMRFL 120

Query: 121 DLLSNRYVKLDNANRALRNEVNWLDFRIHEVAQSYTLEYSHTVGFPVSGVIKVVMSPDSV 180
             LS RYVK D  N   R EVN LDFRI+EVA+SYTL Y  T G  V  + KVVM PD  
Sbjct: 121 HPLSTRYVKYD--NEVFRKEVNLLDFRIYEVAKSYTLGY--TNGALVPRITKVVMFPDCP 180

Query: 181 WTDLRNCTIVAVYDGGKLGFAKHGDDSWTLIDDQNFQYEDVIVYKGQVCAVDIRGTVSWI 240
           W D++ CTIVAVY GGKLGFAKHGD  WTLIDD+NF Y+DVIVYKGQ  AVD  GT+ WI
Sbjct: 181 WIDVKYCTIVAVYAGGKLGFAKHGDYKWTLIDDRNFHYDDVIVYKGQFYAVDRWGTIFWI 240

Query: 241 DSSMKLEQSSSELPD-GNPTHLVESGGKLYAV-----QELMIVN------YW-------- 300
           DSSM+L Q S  L   GN  HLVES  +LY V     +E ++ N      +W        
Sbjct: 241 DSSMRLVQFSPPLCGFGNQKHLVESDDELYVVDRFLDKEPLLWNADIFHIHWLNNLIEDS 300

Query: 301 --EVIDIKVHRLIQKRGRWEEVKNLGDQAFVIGKGCCFSVSAPDFKGLKGNRIYFAKARR 360
             +VID KVHRL Q+ GRW EV+NLG+Q+FV+G  CCFS+SAP F+G+KG+ IYF  A R
Sbjct: 301 PPKVIDFKVHRLDQEWGRWVEVRNLGNQSFVLGNDCCFSISAPKFEGVKGSCIYFTHAPR 360

Query: 361 YNVRHRCYAFDLEKRRVSEASIHGNAQIFEPPRIF 366
             + +  + F+L+ +R+++ASIH N  IF PP I+
Sbjct: 361 STLGYNTHYFELKYKRIAKASIHDNEPIFGPPPIW 388

BLAST of Spg012898 vs. NCBI nr
Match: KAG6594746.1 (putative F-box protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 398.7 bits (1023), Expect = 5.8e-107
Identity = 220/395 (55.70%), Postives = 266/395 (67.34%), Query Frame = 0

Query: 1   MEKYRVRWSDLPPELWPLIGKHLDTYIDVLRFRSVCRSWRASLPPLNAIS---PLHFPYY 60
           M+  RV+WSDLPPE+ P+IGK L+TYIDVLRFRSVCRSWRASLPP N +S   PL  P  
Sbjct: 1   MDDSRVQWSDLPPEILPIIGKSLETYIDVLRFRSVCRSWRASLPPFNIVSSLLPLDLPSP 60

Query: 61  SYSTTAANRRIDALLFRRIIYHLTPL-----HDDRISSSSAAEGWFAMVESTEPGKMRFQ 120
            ++   A+   DALL RR+IY L+PL      D   SSS AAEGW A VEST+ G MRF 
Sbjct: 61  IFT---ADHLTDALLIRRVIYRLSPLDHQHSSDSASSSSFAAEGWLAKVESTKLGTMRFL 120

Query: 121 DLLSNRYVKLDNANRALRNEVNWLDFRIHEVAQSYTLEYSHTVGFPVSGVIKVVMSPDSV 180
             LS RYVK D  N   R EVN LDFRI+EVA+SYTL Y  T G  V  + KVVM PD  
Sbjct: 121 HPLSTRYVKYD--NEVFRKEVNLLDFRIYEVAKSYTLGY--TNGALVPRITKVVMFPDCP 180

Query: 181 WTDLRNCTIVAVYDGGKLGFAKHGDDSWTLIDDQNFQYEDVIVYKGQVCAVDIRGTVSWI 240
           W D++ CTIVAVY GGKLGFAKHGD  WTLIDD+NF Y+DVIVYKGQ  AVD  GT+ WI
Sbjct: 181 WIDVKYCTIVAVYAGGKLGFAKHGDYKWTLIDDRNFHYDDVIVYKGQFYAVDRWGTIFWI 240

Query: 241 DSSMKLEQSSSELPD-GNPTHLVESGGKLYAV-----QELMIVN------YW-------- 300
           DSSM+L Q S  L   GN  HLVES  +LY V     +E ++ N      +W        
Sbjct: 241 DSSMRLVQFSPPLCGFGNQKHLVESDDELYVVDRFLDKEPLLWNADIFHIHWLNNLIEDS 300

Query: 301 --EVIDIKVHRLIQKRGRWEEVKNLGDQAFVIGKGCCFSVSAPDFKGLKGNRIYFAKARR 360
             +VID KVHRL Q+ GRW EV+NLG+Q+FV+G  CCFS+SAP F+G+KG+ IYF  A R
Sbjct: 301 PPKVIDFKVHRLDQEWGRWVEVRNLGNQSFVLGNDCCFSISAPKFEGVKGSCIYFTHAPR 360

Query: 361 YNVRHRCYAFDLEKRRVSEASIHGNAQIFEPPRIF 366
             + +  + F+L+ +R+++ASIH N  IF PP I+
Sbjct: 361 STLGYNTHYFELKYKRIAKASIHDNEPIFGPPPIW 388

BLAST of Spg012898 vs. NCBI nr
Match: XP_022926986.1 (putative F-box protein At1g65770 isoform X1 [Cucurbita moschata])

HSP 1 Score: 397.1 bits (1019), Expect = 1.7e-106
Identity = 219/395 (55.44%), Postives = 266/395 (67.34%), Query Frame = 0

Query: 1   MEKYRVRWSDLPPELWPLIGKHLDTYIDVLRFRSVCRSWRASLPPLNAIS---PLHFPYY 60
           M+  RV+WSDLPPE+ P+IGK L+TYI+VLRFRSVCRSWRASLPP N +S   PL  P  
Sbjct: 1   MDDSRVQWSDLPPEILPIIGKSLETYIEVLRFRSVCRSWRASLPPFNIVSSLLPLDLPSP 60

Query: 61  SYSTTAANRRIDALLFRRIIYHLTPL-----HDDRISSSSAAEGWFAMVESTEPGKMRFQ 120
            ++   A+   DALL RR+IY L+PL      D   SSS AAEGW A VEST+ G MRF 
Sbjct: 61  IFT---ADHLTDALLIRRVIYRLSPLDHQHSSDSASSSSFAAEGWLAKVESTKLGTMRFL 120

Query: 121 DLLSNRYVKLDNANRALRNEVNWLDFRIHEVAQSYTLEYSHTVGFPVSGVIKVVMSPDSV 180
             LS RYVK D  N   R EVN LDFRI+EVA+SYTL Y  T G  V  + KVVM PD  
Sbjct: 121 HPLSTRYVKYD--NEVFRKEVNLLDFRIYEVAKSYTLGY--TNGALVPRITKVVMFPDCP 180

Query: 181 WTDLRNCTIVAVYDGGKLGFAKHGDDSWTLIDDQNFQYEDVIVYKGQVCAVDIRGTVSWI 240
           W D++ CTIVAVY GGKLGFAKHGD  WTLIDD+NF Y+DVIVYKGQ  AVD  GT+ WI
Sbjct: 181 WIDVKYCTIVAVYAGGKLGFAKHGDYKWTLIDDRNFHYDDVIVYKGQFYAVDRWGTIFWI 240

Query: 241 DSSMKLEQSSSELPD-GNPTHLVESGGKLYAV-----QELMIVN------YW-------- 300
           DSSM+L Q S  L   GN  HLVES  +LY V     +E ++ N      +W        
Sbjct: 241 DSSMRLVQFSPPLCGFGNQKHLVESDDELYVVDRFLDKEPLLWNADIFHIHWLNNLIEDS 300

Query: 301 --EVIDIKVHRLIQKRGRWEEVKNLGDQAFVIGKGCCFSVSAPDFKGLKGNRIYFAKARR 360
             +VID KVHRL Q+ GRW EV+NLG+Q+FV+G  CCFS+SAP F+G+KG+ IYF  A R
Sbjct: 301 PPKVIDFKVHRLDQEWGRWVEVRNLGNQSFVLGNDCCFSISAPKFEGVKGSCIYFTHAPR 360

Query: 361 YNVRHRCYAFDLEKRRVSEASIHGNAQIFEPPRIF 366
             + +  + F+L+ +R+++ASIH N  IF PP I+
Sbjct: 361 STLGYNTHYFELKYKRIAKASIHDNEPIFGPPPIW 388

BLAST of Spg012898 vs. ExPASy Swiss-Prot
Match: Q9LVG8 (Putative F-box protein At5g60060 OS=Arabidopsis thaliana OX=3702 GN=At5g60060 PE=4 SV=1)

HSP 1 Score: 142.9 bits (359), Expect = 7.6e-33
Identity = 111/383 (28.98%), Postives = 180/383 (47.00%), Query Frame = 0

Query: 7   RWSDLPPELWPLIGKHLD----TYIDVLRFRSVCRSWRASLPPLNAIS-----PLHFPYY 66
           +WSDLP ++  LI   LD      I +L  RSVC +WR SLP  N  +     P + P++
Sbjct: 10  QWSDLPLDILELISDRLDHDSSDTIHLLCLRSVCATWRLSLPLSNKNNRLSKFPKYLPFW 69

Query: 67  SYSTTAANRRIDALLFRRIIYHLTPLHDDRISSSSAAEGWFAMVESTEPGKMRFQDLLSN 126
           S S++++             + L   +  ++ +          ++ T PG MR  DL SN
Sbjct: 70  SSSSSSSG-----------FFTLKQSNVYKLEAPLNPRTCLVKLQETTPGIMRVLDLFSN 129

Query: 127 RYVKLDNANRALRNEVNWLDFRIHEVAQSYTLEYSHTVGFPVSGVIKVVMSPDSVWTDLR 186
             +     N    ++++  +F +  V ++Y +EY++  G  V     +      + +   
Sbjct: 130 DRICFLPEN--FPSKIDLQEFHVRLVRRTYRMEYANNGGGAVPCFWSLHSDKVVILSSGE 189

Query: 187 NCTIVAVYDGGKLGFAKHG-DDSWTLIDDQ-NFQYEDVIVYKGQVC-AVDIRGTVSWIDS 246
           +  I+A++ GGKLGF K G D+ W ++D+  N  YED+++YK   C  VD +G     D 
Sbjct: 190 DSAIIAIHSGGKLGFLKSGNDEKWKILDNSWNVIYEDIMLYKDNRCIVVDDKGKTVIYDV 249

Query: 247 SMKLEQSSSEL--PDGNPTHLVE-SGGKLYAVQELMIVNYW-------EVIDIKVHRLIQ 306
             K+   +  L    G+  HLVE SGG+++ V +  +   W        V++ +V+ L +
Sbjct: 250 DFKVSDLAEGLVGGGGHKKHLVECSGGEVFLVDK-YVKTVWCKSDISKSVVEFRVYNLKR 309

Query: 307 KRGRWEEVKNLGDQAFVIGKGCCFSVSAPDFKGLKGNRIYFAKARRYNVRHRCYAFDLEK 366
           +  RWEEV++LGD A  IG  C FSV  P    L G  I+++  R    R R    D + 
Sbjct: 310 EEKRWEEVRDLGDVALFIGDDCSFSVQNP-AGDLAGGFIFYSDYRN-GGRSRGICSDGD- 369

Query: 367 RRVSEASIHGNAQIFEPPRIFGP 368
             V    + G+      P  FGP
Sbjct: 370 -GVFNVDMQGDFVFSIKPNYFGP 374

BLAST of Spg012898 vs. ExPASy Swiss-Prot
Match: Q3EBZ2 (F-box protein SKIP23 OS=Arabidopsis thaliana OX=3702 GN=SKIP23 PE=1 SV=1)

HSP 1 Score: 125.9 bits (315), Expect = 9.6e-28
Identity = 99/351 (28.21%), Postives = 161/351 (45.87%), Query Frame = 0

Query: 6   VRWSDLPPELWPLIGKHLDTYIDVLRFRSVCRSWRASLPPLNAISPLHFPYY--SYSTTA 65
           V WS LP +L  LI K L++  D+++FRSVC SWR++  P + +   H P    +  +  
Sbjct: 2   VDWSTLPKDLLDLISKSLESSFDLIQFRSVCSSWRSAAEPKSPLPTHHLPILPDNGGSLF 61

Query: 66  ANRRIDALLFRRIIYHLTPLHDDRISSSSAAEGWFAMVES--TEPGKMRFQDLLSNRYVK 125
            +  +   L +R I  + P H+  I S S   GW   VE     P K+   D L      
Sbjct: 62  PDSAVGFRLSQRSILLIKP-HEPCIESDSF--GWLIKVEEDLNVPRKVTLLDPL------ 121

Query: 126 LDNANRALRNEVNWLD---FRIHEVAQSYTLEYSHTVGFPVSGVI--KVVMSPDSVWTDL 185
            D  N    N    LD   F++ E+ + + L Y +TVG  V  +   K V+       D 
Sbjct: 122 CDTRNSIPENFPRVLDMSKFKVRELGREFKLHYFNTVGDIVESLYLEKAVVKYLDCDGDY 181

Query: 186 RNCTIVAVYDGGKLGFAKHGDDSWTLIDDQNFQYEDVIVYKGQVCAVDIRGTVSWID-SS 245
           +   ++ ++  GKL   +  D +WT+I+D    Y+DV+++ G+  AVD  G    +D SS
Sbjct: 182 K-FVLLTIHVSGKLAVFRSWDRAWTVINDMPSPYDDVMLFDGRFFAVDNNGRTVVVDYSS 241

Query: 246 MKLEQSSSELPDGNPTHLVESGGKLYAVQELMIVNYWE--------------------VI 305
           +KL   +S +  G+   L+ES G++  V   + +   E                     +
Sbjct: 242 LKLTLVASPVFGGDKKFLIESCGEMLLVDMYLSLEAVEGDPGFVEEIFEHPAFYMNERTV 301

Query: 306 DIKVHRLIQKRGRWEEVKNLGDQAFVIGKGCCFSVSAPDFKGL-KGNRIYF 326
             KV+R +++   W +V +L D+   +G    FS SA D   L  G+ ++F
Sbjct: 302 KFKVYRFVEREESWVDVYDLEDKMLFLGDDSTFSASASDILPLCDGSSVFF 342

BLAST of Spg012898 vs. ExPASy Swiss-Prot
Match: Q9LS04 (Putative F-box protein At3g25750 OS=Arabidopsis thaliana OX=3702 GN=At3g25750 PE=4 SV=2)

HSP 1 Score: 125.6 bits (314), Expect = 1.2e-27
Identity = 94/336 (27.98%), Postives = 157/336 (46.73%), Query Frame = 0

Query: 5   RVRWSDLPPELWPLIGKHLDTYIDVLRFRSVCRSWRASLPPLNAISPLHFPYYSYSTTAA 64
           +  WSDLP EL  LI     + IDVLR RS C+SWR+++    +   L F +  Y  T +
Sbjct: 3   KTEWSDLPEELLDLIANRYSSNIDVLRIRSTCKSWRSAV--AMSKERLQFRFERYLPT-S 62

Query: 65  NRRIDALLFRRIIYHLTPLHDDRISSSSAAEGWFAMVESTEPGKMRFQDLLSNRYVKLDN 124
           N++I A L     + +T      + SS   +GW  +V + +  KM ++ +     +  + 
Sbjct: 63  NKKIKAHLSPTTFFRIT------LPSSCPNKGW--LVRTRQASKM-YRKITLLCPLSGER 122

Query: 125 ANRALRNEVNWLDFRIHEVAQSYTLEYSHTVGFPVSGVIKVVMSPDSVWTDLRNCTIV-- 184
             R+    ++ L   + E+ QSY ++      F      K+ +  +     ++N   +  
Sbjct: 123 ITRS-HQTLDLLKVGVSEIRQSYEIQI-----FDGLKDEKIPLDSEIFSNYIKNSDKIPS 182

Query: 185 AVYDGGKLGFAK--HGDDSWTLIDDQNFQYEDVIVYKGQVCAVDIRGTVSWID-SSMKLE 244
             Y   K+   K   G  SWT I +Q   + D+I++ G++ AVD++G + WI  S + + 
Sbjct: 183 VNYHDNKIWCCKTREGSRSWTKIKNQVEDFSDIILHMGRIYAVDLKGAIWWISLSQLTIV 242

Query: 245 QSSSELP-------DGNPTHLVESGGKLYAVQELMIV--NYWEVIDIKVHRLIQKRGRWE 304
           Q +S  P           T LVE  G L  V EL I   +    +  KV+++ +   +W 
Sbjct: 243 QQTSSTPLDYYKYDSCQDTRLVEYCGDLCIVHELSITRNHIQRTVGFKVYKMDEDLAKWV 302

Query: 305 EVKNLGDQAFVIGKGCCFSVSAPDFKGLKGNRIYFA 327
           EV  LGD   ++    CF+V A ++ G   N IYF+
Sbjct: 303 EVSCLGDNTLIVACNSCFTVVASEYHGCLKNSIYFS 320

BLAST of Spg012898 vs. ExPASy Swiss-Prot
Match: Q1PEZ8 (F-box protein At2g26160 OS=Arabidopsis thaliana OX=3702 GN=At2g26160 PE=2 SV=1)

HSP 1 Score: 123.2 bits (308), Expect = 6.2e-27
Identity = 93/343 (27.11%), Postives = 166/343 (48.40%), Query Frame = 0

Query: 8   WSDLPPELWPLIGKHLDTYIDVLRFRSVCRSWRASLPPLNAISPLHFPYYSYSTTAANRR 67
           WS+LP +L  L      +  DVLR RS+C+ WR++     A +P  F     +  ++N+ 
Sbjct: 6   WSELPGDLINLTANRFSSISDVLRVRSICKPWRSA-----AATPKSF---QCNLPSSNKM 65

Query: 68  IDALLFRRIIYHLTPLHDDRISSSSAAEGWFAMVESTEPGKMRFQDLLSNRYVKLDNANR 127
           I+ +L     + +T        SS + +GW  ++ + +  +    +LLS  + +L   + 
Sbjct: 66  IETVLSPTTFFRVTG------PSSCSYKGW--LIRTKQVSESSKINLLSPFFRQLLTPS- 125

Query: 128 ALRNEVNWLDFRIHEVAQSYTLEYSHTVGFPVSGVIKVVMSPDSVWTDL--RNCTIVAVY 187
             +  ++ L F + E+ QSY +       + + GVI     P  + + +   +  I AV 
Sbjct: 126 --QQTLDLLKFEVSEIRQSYEIHIFDK--YLIQGVIG-KEGPSHILSRVVFLDNLIFAVG 185

Query: 188 DGGKLGFAKHGDDS---WTLIDDQNFQYEDVIVYKGQVCAVDIRGTVSWID-SSMKLEQS 247
              K+   K G++S   WT I +Q   + D+I++KGQV A+D+ G + WI  S + L Q 
Sbjct: 186 QDDKIWCCKSGEESSRIWTKIKNQVEDFLDIILHKGQVYALDLTGAIWWISLSPLSLLQF 245

Query: 248 SSELP------DGNPTHLVESGGKLYAVQELMIVNYW-----EVIDIKVHRLIQKRGRWE 307
           +  +P      D     LVE  G L  + +L +   +       +  KV+++ +   +W 
Sbjct: 246 TPSIPMDYDGYDSCNKRLVEYCGDLCIIHQLRLKKAYIRRSQRTVGFKVYKMDEYVAKWV 305

Query: 308 EVKNLGDQAFVIGKGCCFSVSAPDFKGLKGNRIYFAKARRYNV 334
           EV++LGD+A ++ +  CF+V A ++ G   N IYF    R +V
Sbjct: 306 EVRSLGDKALIVARDSCFTVVASEYHGCLNNSIYFVDNVRKSV 326

BLAST of Spg012898 vs. ExPASy Swiss-Prot
Match: Q9SHX9 (Putative F-box protein At1g65770 OS=Arabidopsis thaliana OX=3702 GN=At1g65770 PE=4 SV=1)

HSP 1 Score: 122.9 bits (307), Expect = 8.1e-27
Identity = 95/340 (27.94%), Postives = 156/340 (45.88%), Query Frame = 0

Query: 8   WSDLPPELWPLIGKHLDTYIDVLRFRSVCRSWRASLPPLNAISPLHFPYYSYSTTAAN-- 67
           WS LP +L  +I   L + I++ RFRS+CRSWR+S+P     +P             N  
Sbjct: 4   WSTLPVDLLNMIAGRLFSNIELKRFRSICRSWRSSVPGAGKKNPFRTRPLILLNPNPNKP 63

Query: 68  ----RRIDALLFRRIIYHLTPLHDDRISSSSAAEGWFAMVE-STEPGKMRFQDLLSNRYV 127
               RR    L R   + +T        SSS ++GW    +     GK+   D LS   +
Sbjct: 64  LTDHRRRGEFLSRSAFFRVT-------LSSSPSQGWLIKSDVDVSSGKLHLLDPLSR--L 123

Query: 128 KLDNANRALRNEVNWLDFRIHEVAQSYTLEYSHT--VGFPVSGVIKVVMSPDSVWTDLRN 187
            ++++    R  V+  +F I E+ ++Y +    T     P+   + +V   +       +
Sbjct: 124 PMEHS----RKRVDLSEFTITEIREAYQVHDWRTRKETRPIFKRVALVKDKEG------D 183

Query: 188 CTIVAVYDGGKLGFAKHGDDSWTLIDDQNFQYEDVIVYKGQVCAVDIRGTVSWIDSSMKL 247
             ++ +   GK+    + D       ++ +++ D+IV+KGQ  A+D  G V WI S +K 
Sbjct: 184 NQVLGIRSTGKM---MYWDIKTWKAKEEGYEFSDIIVHKGQTYALDSIGIVYWIRSDLKF 243

Query: 248 EQSSSELPD-GNPTHLVESGGKLYAVQELMIVNYW------------EVIDIKVHRLIQK 307
            +    + D      LVE  G+ Y V+ L+  + W            + +  KV++   +
Sbjct: 244 IRFGPLVGDWTGDRRLVECCGEFYIVERLVGESTWKRKADDTGYEYAKTVGFKVYKFDDE 303

Query: 308 RGRWEEVKNLGDQAFVIGKGCCFSVSAPDFKGLKGNRIYF 326
           +G+  EVK+LGD+AFVI    CFSV A +F G   N IYF
Sbjct: 304 QGKMMEVKSLGDKAFVIATDTCFSVLAHEFYGCLENAIYF 321

BLAST of Spg012898 vs. ExPASy TrEMBL
Match: A0A5A7SHJ7 (Putative F-box protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G00810 PE=4 SV=1)

HSP 1 Score: 403.7 bits (1036), Expect = 8.8e-109
Identity = 221/400 (55.25%), Postives = 263/400 (65.75%), Query Frame = 0

Query: 1   MEKYRVRWSDLPPELWPLIGKHLDTYIDVLRFRSVCRSWRASLPPLNAISPLHFPYYSYS 60
           ME  RVRWSDLPPELWP+IGK LDTYIDVLRFRSVCRSWRASLPP NA+SPL        
Sbjct: 1   MEDSRVRWSDLPPELWPIIGKRLDTYIDVLRFRSVCRSWRASLPPFNAVSPLLPLDLPSP 60

Query: 61  TTAANRRIDALLFRRIIYHLTPLHDDRI-------------SSSSAAEGWFAMVESTEPG 120
             AA+   DA L RRIIY L+PL   +              SS  AAEGW A VEST+ G
Sbjct: 61  VFAADHLTDAFLIRRIIYRLSPLDHHKTFSFASSSTSSSSSSSYCAAEGWLAKVESTKLG 120

Query: 121 KMRFQDLLSNRYVKLDNANRALRNEVNWLDFRIHEVAQSYTLEYSHTVGFPVSGVIKVVM 180
           KMRF   LS RY K +  +   R EVN LDF I+EVA+SYTL Y  T G PV  + KVVM
Sbjct: 121 KMRFLHPLSTRYAKCN--SDLFRKEVNLLDFGIYEVAKSYTLGY--TNGSPVPRITKVVM 180

Query: 181 SPDSVWTDLRNCTIVAVYDGGKLGFAKHGDDSWTLIDDQNFQYEDVIVYKGQVCAVDIRG 240
            PDS W D++ CTI+AVY GGKLGFAKHGDD WTLID +NF Y+DVIVYKGQ  AVD  G
Sbjct: 181 FPDSPWIDVKKCTILAVYAGGKLGFAKHGDDKWTLIDHRNFHYDDVIVYKGQFYAVDRWG 240

Query: 241 TVSWIDSSMKLEQSSSELPD-GNPTHLVESGGKLYAV-----QELMIVN------YW--- 300
           TV WIDSSM+L Q S  L   GN  HLVE  G+LY V     +E ++ N      +W   
Sbjct: 241 TVFWIDSSMRLVQFSPPLCGFGNQKHLVECNGELYVVDRFLDKEPLLWNADIFHIHWLNN 300

Query: 301 -------EVIDIKVHRLIQKRGRWEEVKNLGDQAFVIGKGCCFSVSAPDFKGLKGNRIYF 360
                  +VID K+HRL Q+ GRW EVKNLG+Q+F++G  CCFSVS P+F+GLKG+ IYF
Sbjct: 301 LIEDSPPKVIDFKLHRLDQEWGRWVEVKNLGNQSFILGNDCCFSVSTPNFEGLKGSCIYF 360

Query: 361 AKARRYNVRHRCYAFDLEKRRVSEASIHGNAQIFEPPRIF 366
               +  + +  + F+LE +R+ +AS + NA IF PP I+
Sbjct: 361 THTPKCALGYNTHVFELEDKRILKASSNDNAPIFRPPPIW 396

BLAST of Spg012898 vs. ExPASy TrEMBL
Match: A0A1S4DT77 (putative F-box protein At1g65770 OS=Cucumis melo OX=3656 GN=LOC103485361 PE=4 SV=1)

HSP 1 Score: 403.7 bits (1036), Expect = 8.8e-109
Identity = 221/400 (55.25%), Postives = 263/400 (65.75%), Query Frame = 0

Query: 1   MEKYRVRWSDLPPELWPLIGKHLDTYIDVLRFRSVCRSWRASLPPLNAISPLHFPYYSYS 60
           ME  RVRWSDLPPELWP+IGK LDTYIDVLRFRSVCRSWRASLPP NA+SPL        
Sbjct: 1   MEDSRVRWSDLPPELWPIIGKRLDTYIDVLRFRSVCRSWRASLPPFNAVSPLLPLDLPSP 60

Query: 61  TTAANRRIDALLFRRIIYHLTPLHDDRI-------------SSSSAAEGWFAMVESTEPG 120
             AA+   DA L RRIIY L+PL   +              SS  AAEGW A VEST+ G
Sbjct: 61  VFAADHLTDAFLIRRIIYRLSPLDHHKTFSFASSSTSSSSSSSYCAAEGWLAKVESTKLG 120

Query: 121 KMRFQDLLSNRYVKLDNANRALRNEVNWLDFRIHEVAQSYTLEYSHTVGFPVSGVIKVVM 180
           KMRF   LS RY K +  +   R EVN LDF I+EVA+SYTL Y  T G PV  + KVVM
Sbjct: 121 KMRFLHPLSTRYAKCN--SDLFRKEVNLLDFGIYEVAKSYTLGY--TNGSPVPRITKVVM 180

Query: 181 SPDSVWTDLRNCTIVAVYDGGKLGFAKHGDDSWTLIDDQNFQYEDVIVYKGQVCAVDIRG 240
            PDS W D++ CTI+AVY GGKLGFAKHGDD WTLID +NF Y+DVIVYKGQ  AVD  G
Sbjct: 181 FPDSPWIDVKKCTILAVYAGGKLGFAKHGDDKWTLIDHRNFHYDDVIVYKGQFYAVDRWG 240

Query: 241 TVSWIDSSMKLEQSSSELPD-GNPTHLVESGGKLYAV-----QELMIVN------YW--- 300
           TV WIDSSM+L Q S  L   GN  HLVE  G+LY V     +E ++ N      +W   
Sbjct: 241 TVFWIDSSMRLVQFSPPLCGFGNQKHLVECNGELYVVDRFLDKEPLLWNADIFHIHWLNN 300

Query: 301 -------EVIDIKVHRLIQKRGRWEEVKNLGDQAFVIGKGCCFSVSAPDFKGLKGNRIYF 360
                  +VID K+HRL Q+ GRW EVKNLG+Q+F++G  CCFSVS P+F+GLKG+ IYF
Sbjct: 301 LIEDSPPKVIDFKLHRLDQEWGRWVEVKNLGNQSFILGNDCCFSVSTPNFEGLKGSCIYF 360

Query: 361 AKARRYNVRHRCYAFDLEKRRVSEASIHGNAQIFEPPRIF 366
               +  + +  + F+LE +R+ +AS + NA IF PP I+
Sbjct: 361 THTPKCALGYNTHVFELEDKRILKASSNDNAPIFRPPPIW 396

BLAST of Spg012898 vs. ExPASy TrEMBL
Match: A0A6J1EGF3 (putative F-box protein At1g65770 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111433939 PE=4 SV=1)

HSP 1 Score: 397.1 bits (1019), Expect = 8.2e-107
Identity = 219/395 (55.44%), Postives = 266/395 (67.34%), Query Frame = 0

Query: 1   MEKYRVRWSDLPPELWPLIGKHLDTYIDVLRFRSVCRSWRASLPPLNAIS---PLHFPYY 60
           M+  RV+WSDLPPE+ P+IGK L+TYI+VLRFRSVCRSWRASLPP N +S   PL  P  
Sbjct: 1   MDDSRVQWSDLPPEILPIIGKSLETYIEVLRFRSVCRSWRASLPPFNIVSSLLPLDLPSP 60

Query: 61  SYSTTAANRRIDALLFRRIIYHLTPL-----HDDRISSSSAAEGWFAMVESTEPGKMRFQ 120
            ++   A+   DALL RR+IY L+PL      D   SSS AAEGW A VEST+ G MRF 
Sbjct: 61  IFT---ADHLTDALLIRRVIYRLSPLDHQHSSDSASSSSFAAEGWLAKVESTKLGTMRFL 120

Query: 121 DLLSNRYVKLDNANRALRNEVNWLDFRIHEVAQSYTLEYSHTVGFPVSGVIKVVMSPDSV 180
             LS RYVK D  N   R EVN LDFRI+EVA+SYTL Y  T G  V  + KVVM PD  
Sbjct: 121 HPLSTRYVKYD--NEVFRKEVNLLDFRIYEVAKSYTLGY--TNGALVPRITKVVMFPDCP 180

Query: 181 WTDLRNCTIVAVYDGGKLGFAKHGDDSWTLIDDQNFQYEDVIVYKGQVCAVDIRGTVSWI 240
           W D++ CTIVAVY GGKLGFAKHGD  WTLIDD+NF Y+DVIVYKGQ  AVD  GT+ WI
Sbjct: 181 WIDVKYCTIVAVYAGGKLGFAKHGDYKWTLIDDRNFHYDDVIVYKGQFYAVDRWGTIFWI 240

Query: 241 DSSMKLEQSSSELPD-GNPTHLVESGGKLYAV-----QELMIVN------YW-------- 300
           DSSM+L Q S  L   GN  HLVES  +LY V     +E ++ N      +W        
Sbjct: 241 DSSMRLVQFSPPLCGFGNQKHLVESDDELYVVDRFLDKEPLLWNADIFHIHWLNNLIEDS 300

Query: 301 --EVIDIKVHRLIQKRGRWEEVKNLGDQAFVIGKGCCFSVSAPDFKGLKGNRIYFAKARR 360
             +VID KVHRL Q+ GRW EV+NLG+Q+FV+G  CCFS+SAP F+G+KG+ IYF  A R
Sbjct: 301 PPKVIDFKVHRLDQEWGRWVEVRNLGNQSFVLGNDCCFSISAPKFEGVKGSCIYFTHAPR 360

Query: 361 YNVRHRCYAFDLEKRRVSEASIHGNAQIFEPPRIF 366
             + +  + F+L+ +R+++ASIH N  IF PP I+
Sbjct: 361 STLGYNTHYFELKYKRIAKASIHDNEPIFGPPPIW 388

BLAST of Spg012898 vs. ExPASy TrEMBL
Match: A0A6J1KNW7 (putative F-box protein At1g65770 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111497348 PE=4 SV=1)

HSP 1 Score: 396.4 bits (1017), Expect = 1.4e-106
Identity = 219/395 (55.44%), Postives = 265/395 (67.09%), Query Frame = 0

Query: 1   MEKYRVRWSDLPPELWPLIGKHLDTYIDVLRFRSVCRSWRASLPPLNAIS---PLHFPYY 60
           M+  RV+WSDLPPE+ P+IGK L+TYIDVLRFRSVCRSWRASLPP N +S   PL  P  
Sbjct: 1   MDDSRVQWSDLPPEILPIIGKSLETYIDVLRFRSVCRSWRASLPPFNIVSSLLPLDLPSP 60

Query: 61  SYSTTAANRRIDALLFRRIIYHLTPL-----HDDRISSSSAAEGWFAMVESTEPGKMRFQ 120
            ++   A+   DALL RR+IY L+PL      D   SSS AAEGW A VEST+ G MRF 
Sbjct: 61  IFT---ADHLTDALLIRRVIYRLSPLDHQHSSDSASSSSFAAEGWLAKVESTKLGTMRFL 120

Query: 121 DLLSNRYVKLDNANRALRNEVNWLDFRIHEVAQSYTLEYSHTVGFPVSGVIKVVMSPDSV 180
             LS RYVK D  N   R EVN LDFRI+EVA+SYTL Y +  G  V  + KVVM PD  
Sbjct: 121 HPLSTRYVKYD--NEVFRKEVNLLDFRIYEVAKSYTLGYIN--GALVPRITKVVMFPDCP 180

Query: 181 WTDLRNCTIVAVYDGGKLGFAKHGDDSWTLIDDQNFQYEDVIVYKGQVCAVDIRGTVSWI 240
           W D++ CTIVAVY GGKLGFAKHGD  WTLIDD+NF Y+DVIVYKGQ  AVD  GT+ WI
Sbjct: 181 WIDVKYCTIVAVYAGGKLGFAKHGDYKWTLIDDRNFHYDDVIVYKGQFYAVDRWGTIFWI 240

Query: 241 DSSMKLEQSSSELPD-GNPTHLVESGGKLYAV-----QELMIVN------YW-------- 300
           DSSM+L Q S  L   GN  H VES  +LY V     +E ++ N      +W        
Sbjct: 241 DSSMRLVQFSPPLCGFGNQKHFVESDDELYVVDRFLDKEPLLWNADIFHIHWLNNLIEDS 300

Query: 301 --EVIDIKVHRLIQKRGRWEEVKNLGDQAFVIGKGCCFSVSAPDFKGLKGNRIYFAKARR 360
             +VID KVHRL Q+ GRW EV+NLG+Q+FV+G  CCFSVSAP F+G+KG+ IYF  A R
Sbjct: 301 PPKVIDFKVHRLDQEWGRWVEVRNLGNQSFVLGNDCCFSVSAPKFEGVKGSCIYFTHAPR 360

Query: 361 YNVRHRCYAFDLEKRRVSEASIHGNAQIFEPPRIF 366
             + +  + F+L+ +R+++ASIH N  IF PP I+
Sbjct: 361 STLGYNTHYFELKYKRIAKASIHDNEPIFGPPPIW 388

BLAST of Spg012898 vs. ExPASy TrEMBL
Match: A0A0A0KJJ1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G507420 PE=4 SV=1)

HSP 1 Score: 392.5 bits (1007), Expect = 2.0e-105
Identity = 221/403 (54.84%), Postives = 261/403 (64.76%), Query Frame = 0

Query: 1   MEKYRVRWSDLPPELWPLIGKHLDTYIDVLRFRSVCRSWRASLPPLNAISPLHFPYYSYS 60
           ME  RVRWSDLPPELWP+IGK LDTYIDVLRFRSVCRSWRASLPP NA+SPL        
Sbjct: 1   MEDSRVRWSDLPPELWPIIGKRLDTYIDVLRFRSVCRSWRASLPPFNAVSPLLPLDLPSP 60

Query: 61  TTAANRRIDALLFRRIIYHLTPLHD----DRISSSSA------------AEGWFAMVEST 120
             AA+   DA L RRIIY L+PL      D  SSSS+            AEGW A VEST
Sbjct: 61  VFAADHLTDAYLIRRIIYRLSPLDHHQTFDFASSSSSSSSSSSSSFSCPAEGWLAKVEST 120

Query: 121 EPGKMRFQDLLSNRYVKLDNANRALRNEVNWLDFRIHEVAQSYTLEYSHTVGFPVSGVIK 180
           + GKMRF   LS RY K +  +  LR EVN LDF I+EVA+SYTL Y  T G  V  + K
Sbjct: 121 KLGKMRFLHPLSTRYAKCN--SDLLRKEVNLLDFGIYEVAKSYTLGY--TNGSLVPRITK 180

Query: 181 VVMSPDSVWTDLRNCTIVAVYDGGKLGFAKHGDDSWTLIDDQNFQYEDVIVYKGQVCAVD 240
           VVM PDS W D++ CTI+A+Y GGKLGFAKHGD+ WTLID  NF Y+DVIVYKGQ  AVD
Sbjct: 181 VVMFPDSPWIDVKKCTILAIYAGGKLGFAKHGDNKWTLIDHCNFHYDDVIVYKGQFYAVD 240

Query: 241 IRGTVSWIDSSMKLEQSSSELPD-GNPTHLVESGGKLYAV-----QELMIVN------YW 300
             GT+ WIDSSMKL Q S  L   GN  HLVE  G+LY V     +E ++ N      +W
Sbjct: 241 RWGTIFWIDSSMKLVQFSPPLCGFGNQKHLVECNGELYVVDRFLDKEPLLWNADIFHIHW 300

Query: 301 ----------EVIDIKVHRLIQKRGRWEEVKNLGDQAFVIGKGCCFSVSAPDFKGLKGNR 360
                     +VID K+HRL Q+ GRW EVKNLG+++FV+G  CCFSVS P FKGLKG+ 
Sbjct: 301 LNNLIEDSSPKVIDFKLHRLDQEWGRWVEVKNLGNESFVLGNDCCFSVSTPYFKGLKGSC 360

Query: 361 IYFAKARRYNVRHRCYAFDLEKRRVSEASIHGNAQIFEPPRIF 366
           IYF    +  + +    F+LE++R+  A  + NA IF PP I+
Sbjct: 361 IYFTHTPKCALGYNTLVFELEEKRILNAFSNDNAPIFRPPPIW 399

BLAST of Spg012898 vs. TAIR 10
Match: AT5G60060.1 (Protein of unknown function (DUF295) )

HSP 1 Score: 142.9 bits (359), Expect = 5.4e-34
Identity = 111/383 (28.98%), Postives = 180/383 (47.00%), Query Frame = 0

Query: 7   RWSDLPPELWPLIGKHLD----TYIDVLRFRSVCRSWRASLPPLNAIS-----PLHFPYY 66
           +WSDLP ++  LI   LD      I +L  RSVC +WR SLP  N  +     P + P++
Sbjct: 10  QWSDLPLDILELISDRLDHDSSDTIHLLCLRSVCATWRLSLPLSNKNNRLSKFPKYLPFW 69

Query: 67  SYSTTAANRRIDALLFRRIIYHLTPLHDDRISSSSAAEGWFAMVESTEPGKMRFQDLLSN 126
           S S++++             + L   +  ++ +          ++ T PG MR  DL SN
Sbjct: 70  SSSSSSSG-----------FFTLKQSNVYKLEAPLNPRTCLVKLQETTPGIMRVLDLFSN 129

Query: 127 RYVKLDNANRALRNEVNWLDFRIHEVAQSYTLEYSHTVGFPVSGVIKVVMSPDSVWTDLR 186
             +     N    ++++  +F +  V ++Y +EY++  G  V     +      + +   
Sbjct: 130 DRICFLPEN--FPSKIDLQEFHVRLVRRTYRMEYANNGGGAVPCFWSLHSDKVVILSSGE 189

Query: 187 NCTIVAVYDGGKLGFAKHG-DDSWTLIDDQ-NFQYEDVIVYKGQVC-AVDIRGTVSWIDS 246
           +  I+A++ GGKLGF K G D+ W ++D+  N  YED+++YK   C  VD +G     D 
Sbjct: 190 DSAIIAIHSGGKLGFLKSGNDEKWKILDNSWNVIYEDIMLYKDNRCIVVDDKGKTVIYDV 249

Query: 247 SMKLEQSSSEL--PDGNPTHLVE-SGGKLYAVQELMIVNYW-------EVIDIKVHRLIQ 306
             K+   +  L    G+  HLVE SGG+++ V +  +   W        V++ +V+ L +
Sbjct: 250 DFKVSDLAEGLVGGGGHKKHLVECSGGEVFLVDK-YVKTVWCKSDISKSVVEFRVYNLKR 309

Query: 307 KRGRWEEVKNLGDQAFVIGKGCCFSVSAPDFKGLKGNRIYFAKARRYNVRHRCYAFDLEK 366
           +  RWEEV++LGD A  IG  C FSV  P    L G  I+++  R    R R    D + 
Sbjct: 310 EEKRWEEVRDLGDVALFIGDDCSFSVQNP-AGDLAGGFIFYSDYRN-GGRSRGICSDGD- 369

Query: 367 RRVSEASIHGNAQIFEPPRIFGP 368
             V    + G+      P  FGP
Sbjct: 370 -GVFNVDMQGDFVFSIKPNYFGP 374

BLAST of Spg012898 vs. TAIR 10
Match: AT2G17030.1 (F-box family protein with a domain of unknown function (DUF295) )

HSP 1 Score: 125.9 bits (315), Expect = 6.8e-29
Identity = 99/351 (28.21%), Postives = 161/351 (45.87%), Query Frame = 0

Query: 6   VRWSDLPPELWPLIGKHLDTYIDVLRFRSVCRSWRASLPPLNAISPLHFPYY--SYSTTA 65
           V WS LP +L  LI K L++  D+++FRSVC SWR++  P + +   H P    +  +  
Sbjct: 2   VDWSTLPKDLLDLISKSLESSFDLIQFRSVCSSWRSAAEPKSPLPTHHLPILPDNGGSLF 61

Query: 66  ANRRIDALLFRRIIYHLTPLHDDRISSSSAAEGWFAMVES--TEPGKMRFQDLLSNRYVK 125
            +  +   L +R I  + P H+  I S S   GW   VE     P K+   D L      
Sbjct: 62  PDSAVGFRLSQRSILLIKP-HEPCIESDSF--GWLIKVEEDLNVPRKVTLLDPL------ 121

Query: 126 LDNANRALRNEVNWLD---FRIHEVAQSYTLEYSHTVGFPVSGVI--KVVMSPDSVWTDL 185
            D  N    N    LD   F++ E+ + + L Y +TVG  V  +   K V+       D 
Sbjct: 122 CDTRNSIPENFPRVLDMSKFKVRELGREFKLHYFNTVGDIVESLYLEKAVVKYLDCDGDY 181

Query: 186 RNCTIVAVYDGGKLGFAKHGDDSWTLIDDQNFQYEDVIVYKGQVCAVDIRGTVSWID-SS 245
           +   ++ ++  GKL   +  D +WT+I+D    Y+DV+++ G+  AVD  G    +D SS
Sbjct: 182 K-FVLLTIHVSGKLAVFRSWDRAWTVINDMPSPYDDVMLFDGRFFAVDNNGRTVVVDYSS 241

Query: 246 MKLEQSSSELPDGNPTHLVESGGKLYAVQELMIVNYWE--------------------VI 305
           +KL   +S +  G+   L+ES G++  V   + +   E                     +
Sbjct: 242 LKLTLVASPVFGGDKKFLIESCGEMLLVDMYLSLEAVEGDPGFVEEIFEHPAFYMNERTV 301

Query: 306 DIKVHRLIQKRGRWEEVKNLGDQAFVIGKGCCFSVSAPDFKGL-KGNRIYF 326
             KV+R +++   W +V +L D+   +G    FS SA D   L  G+ ++F
Sbjct: 302 KFKVYRFVEREESWVDVYDLEDKMLFLGDDSTFSASASDILPLCDGSSVFF 342

BLAST of Spg012898 vs. TAIR 10
Match: AT2G26160.1 (F-box family protein with a domain of unknown function (DUF295) )

HSP 1 Score: 123.2 bits (308), Expect = 4.4e-28
Identity = 93/343 (27.11%), Postives = 166/343 (48.40%), Query Frame = 0

Query: 8   WSDLPPELWPLIGKHLDTYIDVLRFRSVCRSWRASLPPLNAISPLHFPYYSYSTTAANRR 67
           WS+LP +L  L      +  DVLR RS+C+ WR++     A +P  F     +  ++N+ 
Sbjct: 6   WSELPGDLINLTANRFSSISDVLRVRSICKPWRSA-----AATPKSF---QCNLPSSNKM 65

Query: 68  IDALLFRRIIYHLTPLHDDRISSSSAAEGWFAMVESTEPGKMRFQDLLSNRYVKLDNANR 127
           I+ +L     + +T        SS + +GW  ++ + +  +    +LLS  + +L   + 
Sbjct: 66  IETVLSPTTFFRVTG------PSSCSYKGW--LIRTKQVSESSKINLLSPFFRQLLTPS- 125

Query: 128 ALRNEVNWLDFRIHEVAQSYTLEYSHTVGFPVSGVIKVVMSPDSVWTDL--RNCTIVAVY 187
             +  ++ L F + E+ QSY +       + + GVI     P  + + +   +  I AV 
Sbjct: 126 --QQTLDLLKFEVSEIRQSYEIHIFDK--YLIQGVIG-KEGPSHILSRVVFLDNLIFAVG 185

Query: 188 DGGKLGFAKHGDDS---WTLIDDQNFQYEDVIVYKGQVCAVDIRGTVSWID-SSMKLEQS 247
              K+   K G++S   WT I +Q   + D+I++KGQV A+D+ G + WI  S + L Q 
Sbjct: 186 QDDKIWCCKSGEESSRIWTKIKNQVEDFLDIILHKGQVYALDLTGAIWWISLSPLSLLQF 245

Query: 248 SSELP------DGNPTHLVESGGKLYAVQELMIVNYW-----EVIDIKVHRLIQKRGRWE 307
           +  +P      D     LVE  G L  + +L +   +       +  KV+++ +   +W 
Sbjct: 246 TPSIPMDYDGYDSCNKRLVEYCGDLCIIHQLRLKKAYIRRSQRTVGFKVYKMDEYVAKWV 305

Query: 308 EVKNLGDQAFVIGKGCCFSVSAPDFKGLKGNRIYFAKARRYNV 334
           EV++LGD+A ++ +  CF+V A ++ G   N IYF    R +V
Sbjct: 306 EVRSLGDKALIVARDSCFTVVASEYHGCLNNSIYFVDNVRKSV 326

BLAST of Spg012898 vs. TAIR 10
Match: AT1G65770.1 (ascorbic acid mannose pathway regulator 1 )

HSP 1 Score: 122.9 bits (307), Expect = 5.7e-28
Identity = 95/340 (27.94%), Postives = 156/340 (45.88%), Query Frame = 0

Query: 8   WSDLPPELWPLIGKHLDTYIDVLRFRSVCRSWRASLPPLNAISPLHFPYYSYSTTAAN-- 67
           WS LP +L  +I   L + I++ RFRS+CRSWR+S+P     +P             N  
Sbjct: 4   WSTLPVDLLNMIAGRLFSNIELKRFRSICRSWRSSVPGAGKKNPFRTRPLILLNPNPNKP 63

Query: 68  ----RRIDALLFRRIIYHLTPLHDDRISSSSAAEGWFAMVE-STEPGKMRFQDLLSNRYV 127
               RR    L R   + +T        SSS ++GW    +     GK+   D LS   +
Sbjct: 64  LTDHRRRGEFLSRSAFFRVT-------LSSSPSQGWLIKSDVDVSSGKLHLLDPLSR--L 123

Query: 128 KLDNANRALRNEVNWLDFRIHEVAQSYTLEYSHT--VGFPVSGVIKVVMSPDSVWTDLRN 187
            ++++    R  V+  +F I E+ ++Y +    T     P+   + +V   +       +
Sbjct: 124 PMEHS----RKRVDLSEFTITEIREAYQVHDWRTRKETRPIFKRVALVKDKEG------D 183

Query: 188 CTIVAVYDGGKLGFAKHGDDSWTLIDDQNFQYEDVIVYKGQVCAVDIRGTVSWIDSSMKL 247
             ++ +   GK+    + D       ++ +++ D+IV+KGQ  A+D  G V WI S +K 
Sbjct: 184 NQVLGIRSTGKM---MYWDIKTWKAKEEGYEFSDIIVHKGQTYALDSIGIVYWIRSDLKF 243

Query: 248 EQSSSELPD-GNPTHLVESGGKLYAVQELMIVNYW------------EVIDIKVHRLIQK 307
            +    + D      LVE  G+ Y V+ L+  + W            + +  KV++   +
Sbjct: 244 IRFGPLVGDWTGDRRLVECCGEFYIVERLVGESTWKRKADDTGYEYAKTVGFKVYKFDDE 303

Query: 308 RGRWEEVKNLGDQAFVIGKGCCFSVSAPDFKGLKGNRIYF 326
           +G+  EVK+LGD+AFVI    CFSV A +F G   N IYF
Sbjct: 304 QGKMMEVKSLGDKAFVIATDTCFSVLAHEFYGCLENAIYF 321

BLAST of Spg012898 vs. TAIR 10
Match: AT3G25750.1 (F-box family protein with a domain of unknown function (DUF295) )

HSP 1 Score: 122.9 bits (307), Expect = 5.7e-28
Identity = 93/332 (28.01%), Postives = 152/332 (45.78%), Query Frame = 0

Query: 5   RVRWSDLPPELWPLIGKHLDTYIDVLRFRSVCRSWRASLPPLNAISPLHFPYYSYSTTAA 64
           +  WSDLP EL  LI     + IDVLR RS C+SWR+++    +   L F +  Y  T +
Sbjct: 3   KTEWSDLPEELLDLIANRYSSNIDVLRIRSTCKSWRSAV--AMSKERLQFRFERYLPT-S 62

Query: 65  NRRIDALLFRRIIYHLTPLHDDRISSSSAAEGWFAMVESTEPGKMRFQDLLSNRYVKLDN 124
           N++I A L     + +T      + SS   +GW  +V + +  KM ++ +     +  + 
Sbjct: 63  NKKIKAHLSPTTFFRIT------LPSSCPNKGW--LVRTRQASKM-YRKITLLCPLSGER 122

Query: 125 ANRALRNEVNWLDFRIHEVAQSYTLEYSHTVGFPVSGVIKVVMSPDSVWTDLRNCTIVAV 184
             R+    ++ L   + E+ QSY ++          G+    +  DS         I + 
Sbjct: 123 ITRS-HQTLDLLKVGVSEIRQSYEIQI-------FDGLKDEKIPLDS--------EIFSN 182

Query: 185 YDGGKLGFAKHGDDSWTLIDDQNFQYEDVIVYKGQVCAVDIRGTVSWID-SSMKLEQSSS 244
           Y          G  SWT I +Q   + D+I++ G++ AVD++G + WI  S + + Q +S
Sbjct: 183 YIKNSDKIPSEGSRSWTKIKNQVEDFSDIILHMGRIYAVDLKGAIWWISLSQLTIVQQTS 242

Query: 245 ELP-------DGNPTHLVESGGKLYAVQELMIV--NYWEVIDIKVHRLIQKRGRWEEVKN 304
             P           T LVE  G L  V EL I   +    +  KV+++ +   +W EV  
Sbjct: 243 STPLDYYKYDSCQDTRLVEYCGDLCIVHELSITRNHIQRTVGFKVYKMDEDLAKWVEVSC 302

Query: 305 LGDQAFVIGKGCCFSVSAPDFKGLKGNRIYFA 327
           LGD   ++    CF+V A ++ G   N IYF+
Sbjct: 303 LGDNTLIVACNSCFTVVASEYHGCLKNSIYFS 306

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038881861.11.5e-11056.02putative F-box protein At1g65770 [Benincasa hispida][more]
XP_016899202.11.8e-10855.25PREDICTED: putative F-box protein At1g65770 [Cucumis melo] >KAA0025628.1 putativ... [more]
XP_023518907.14.5e-10755.70putative F-box protein At1g65770 isoform X1 [Cucurbita pepo subsp. pepo][more]
KAG6594746.15.8e-10755.70putative F-box protein, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022926986.11.7e-10655.44putative F-box protein At1g65770 isoform X1 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q9LVG87.6e-3328.98Putative F-box protein At5g60060 OS=Arabidopsis thaliana OX=3702 GN=At5g60060 PE... [more]
Q3EBZ29.6e-2828.21F-box protein SKIP23 OS=Arabidopsis thaliana OX=3702 GN=SKIP23 PE=1 SV=1[more]
Q9LS041.2e-2727.98Putative F-box protein At3g25750 OS=Arabidopsis thaliana OX=3702 GN=At3g25750 PE... [more]
Q1PEZ86.2e-2727.11F-box protein At2g26160 OS=Arabidopsis thaliana OX=3702 GN=At2g26160 PE=2 SV=1[more]
Q9SHX98.1e-2727.94Putative F-box protein At1g65770 OS=Arabidopsis thaliana OX=3702 GN=At1g65770 PE... [more]
Match NameE-valueIdentityDescription
A0A5A7SHJ78.8e-10955.25Putative F-box protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold2... [more]
A0A1S4DT778.8e-10955.25putative F-box protein At1g65770 OS=Cucumis melo OX=3656 GN=LOC103485361 PE=4 SV... [more]
A0A6J1EGF38.2e-10755.44putative F-box protein At1g65770 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC... [more]
A0A6J1KNW71.4e-10655.44putative F-box protein At1g65770 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
A0A0A0KJJ12.0e-10554.84Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G507420 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT5G60060.15.4e-3428.98Protein of unknown function (DUF295) [more]
AT2G17030.16.8e-2928.21F-box family protein with a domain of unknown function (DUF295) [more]
AT2G26160.14.4e-2827.11F-box family protein with a domain of unknown function (DUF295) [more]
AT1G65770.15.7e-2827.94ascorbic acid mannose pathway regulator 1 [more]
AT3G25750.15.7e-2828.01F-box family protein with a domain of unknown function (DUF295) [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001810F-box domainSMARTSM00256fbox_2coord: 11..52
e-value: 0.0072
score: 25.5
IPR001810F-box domainPFAMPF00646F-boxcoord: 8..41
e-value: 1.1E-4
score: 22.0
NoneNo IPR availableGENE3D1.20.1280.50coord: 2..45
e-value: 5.3E-6
score: 28.2
NoneNo IPR availablePANTHERPTHR47123:SF6ASCORBIC ACID MANNOSE PATHWAY REGULATOR-RELATEDcoord: 3..362
NoneNo IPR availablePANTHERPTHR47123F-BOX PROTEIN SKIP23coord: 3..362
IPR005174Domain unknown function DUF295PFAMPF03478DUF295coord: 290..341
e-value: 5.3E-14
score: 51.6
IPR036047F-box-like domain superfamilySUPERFAMILY81383F-box domaincoord: 6..41

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg012898.1Spg012898.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005515 protein binding