Spg012691 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg012691
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionAAA-ATPase
Locationscaffold1: 5831964 .. 5833614 (-)
RNA-Seq ExpressionSpg012691
SyntenySpg012691
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTCACTTTCAAAGAGATGGTTGTTCCCCAGTCGGCCTCCGCCGTCTTCTCCGCCTATGCCTCCTTCGCCACCACCATCATGCTCATTCGTTCTATAACCAACGAACTCCTCCCCGCTCAATTCCTCTCCTTCTTCTCTACCATTTTCTTCTACTTTCTCGGCAGTTCCATTTCTTCTCACACCAAGTTTGTAATCGATGAGAATTCTGGATTATTCCACTTGAATCAGGTCTTCCAGGCTGCCGAGATTTATCTTCGTACCAAAATCAGCCCTTCTATACAAACTCTCAGGGTCACCAAAACCCCCCAACAGAACAAAATCATGCTTACCATTGATAAGGACCAAGAAATCATCGATTACTTCAACAACATTCGACTCCAATGGCGCTTTGTCTGCCTCCGAGATGAACGCGATGTCAGTAACCCAGAAAAGCAATATTTTGAGCTTTTGTTCCCTACCAAATTTAGAGATACAGTAGTGGATTTCTATTTGCCTTACGTTTTGAGAAGGGCTGAGGAGATTAGAGAGGAGGATAAAGTTGTCAAGATCTATAGCCAGCAATGCGAGGTTTATGATTATTCTAATCCCGGCCCCAAATGTTGGGGGTCTATTATTCTAGACCATCCGGCAACGTTTGAGACGGTGGCCATGGACCCCGACTTGAAGCAGTGGATAATCGACGATTTAGATAGGTTTCTTCGAAGGAAAGATTTCTATAAGAAAGTGGGGAAGGCTTGGAAGAGGGGATATCTTCTGTATGGTCCTCCCGGTACGGGAAAATCAAGCTTAATCGCCGCCATAGCTAACTACCTCAAGTTCGATATCTATGATTTGGATCTCGCGAGCGTTTACAATAATACCATTCTGAGGAGGCTTTTGCTCGCCACCAAGAATCGGTCCATTTTGGTGATTGAAGATATAGATTGCAGCGCCAATCTACAAAATCGGCAGAGTGAGGAACATTTTGATGCATCCAACAGCAACAGCAAGGTGAATTTTCGATTTCTTTTTTTTTTTTTTCTTTCTGTGCCCTAATTGGAATTTCTGAATTCTATTACTCCGAATCGAAAATTCTAATGAAGATTTTGATTATGATGTGCAGCTTACCTTGTCGGGGATGCTTAATTTCATCGACGGATTGTGGTCGAGTTGTGGGGACGAAAGAATCATCATCTTCACGACGAACCACAAGGAGCGATTGGATCCGGCTCTGCTTCGGCCTGGTCGGATGGATGTTCATATAAACATGTCGTATTGTAGCCCTGAAGGGTTCAAGGTTCTGGCCTCTAATTACCTCGGCGGCGAATCGGTTCAGCATCGGCTGTACGGAGAAATCGAAGAGCTAATCAGAGATATGGAAGTATCTCCGGCGGAGATGGCGGAAGAGCTGATGAAGAGCGAGGAGGCCGATGCCGTTCTGGAAGGGCTCGTTGTTTTTCTGAAACGTAAACGGGAAGAACAGAGGAAGGCGAAAGAGGAGAAGGAAAGTGAGAAGAATGAAGAGAGAGAGGAACGAGAAGAAATGGACAAAACTGAAACTGAAGAAGAAGAAGAAGAAGAAGAAGAAAAAGAAAAAGAAGAAGAAGAACCCAAAAAATTAAAATGGGATATGAGAATTAGAAGGACGGGAAGGGGCCGGAGAATTTGA

mRNA sequence

ATGTTCACTTTCAAAGAGATGGTTGTTCCCCAGTCGGCCTCCGCCGTCTTCTCCGCCTATGCCTCCTTCGCCACCACCATCATGCTCATTCGTTCTATAACCAACGAACTCCTCCCCGCTCAATTCCTCTCCTTCTTCTCTACCATTTTCTTCTACTTTCTCGGCAGTTCCATTTCTTCTCACACCAAGTTTGTAATCGATGAGAATTCTGGATTATTCCACTTGAATCAGGTCTTCCAGGCTGCCGAGATTTATCTTCGTACCAAAATCAGCCCTTCTATACAAACTCTCAGGGTCACCAAAACCCCCCAACAGAACAAAATCATGCTTACCATTGATAAGGACCAAGAAATCATCGATTACTTCAACAACATTCGACTCCAATGGCGCTTTGTCTGCCTCCGAGATGAACGCGATGTCAGTAACCCAGAAAAGCAATATTTTGAGCTTTTGTTCCCTACCAAATTTAGAGATACAGTAGTGGATTTCTATTTGCCTTACGTTTTGAGAAGGGCTGAGGAGATTAGAGAGGAGGATAAAGTTGTCAAGATCTATAGCCAGCAATGCGAGGTTTATGATTATTCTAATCCCGGCCCCAAATGTTGGGGGTCTATTATTCTAGACCATCCGGCAACGTTTGAGACGGTGGCCATGGACCCCGACTTGAAGCAGTGGATAATCGACGATTTAGATAGGTTTCTTCGAAGGAAAGATTTCTATAAGAAAGTGGGGAAGGCTTGGAAGAGGGGATATCTTCTGTATGGTCCTCCCGGTACGGGAAAATCAAGCTTAATCGCCGCCATAGCTAACTACCTCAAGTTCGATATCTATGATTTGGATCTCGCGAGCGTTTACAATAATACCATTCTGAGGAGGCTTTTGCTCGCCACCAAGAATCGGTCCATTTTGGTGATTGAAGATATAGATTGCAGCGCCAATCTACAAAATCGGCAGAGTGAGGAACATTTTGATGCATCCAACAGCAACAGCAAGCTTACCTTGTCGGGGATGCTTAATTTCATCGACGGATTGTGGTCGAGTTGTGGGGACGAAAGAATCATCATCTTCACGACGAACCACAAGGAGCGATTGGATCCGGCTCTGCTTCGGCCTGGTCGGATGGATGTTCATATAAACATGTCGTATTGTAGCCCTGAAGGGTTCAAGGTTCTGGCCTCTAATTACCTCGGCGGCGAATCGGTTCAGCATCGGCTGTACGGAGAAATCGAAGAGCTAATCAGAGATATGGAAGTATCTCCGGCGGAGATGGCGGAAGAGCTGATGAAGAGCGAGGAGGCCGATGCCGTTCTGGAAGGGCTCGTTGTTTTTCTGAAACGTAAACGGGAAGAACAGAGGAAGGCGAAAGAGGAGAAGGAAAGTGAGAAGAATGAAGAGAGAGAGGAACGAGAAGAAATGGACAAAACTGAAACTGAAGAAGAAGAAGAAGAAGAAGAAGAAAAAGAAAAAGAAGAAGAAGAACCCAAAAAATTAAAATGGGATATGAGAATTAGAAGGACGGGAAGGGGCCGGAGAATTTGA

Coding sequence (CDS)

ATGTTCACTTTCAAAGAGATGGTTGTTCCCCAGTCGGCCTCCGCCGTCTTCTCCGCCTATGCCTCCTTCGCCACCACCATCATGCTCATTCGTTCTATAACCAACGAACTCCTCCCCGCTCAATTCCTCTCCTTCTTCTCTACCATTTTCTTCTACTTTCTCGGCAGTTCCATTTCTTCTCACACCAAGTTTGTAATCGATGAGAATTCTGGATTATTCCACTTGAATCAGGTCTTCCAGGCTGCCGAGATTTATCTTCGTACCAAAATCAGCCCTTCTATACAAACTCTCAGGGTCACCAAAACCCCCCAACAGAACAAAATCATGCTTACCATTGATAAGGACCAAGAAATCATCGATTACTTCAACAACATTCGACTCCAATGGCGCTTTGTCTGCCTCCGAGATGAACGCGATGTCAGTAACCCAGAAAAGCAATATTTTGAGCTTTTGTTCCCTACCAAATTTAGAGATACAGTAGTGGATTTCTATTTGCCTTACGTTTTGAGAAGGGCTGAGGAGATTAGAGAGGAGGATAAAGTTGTCAAGATCTATAGCCAGCAATGCGAGGTTTATGATTATTCTAATCCCGGCCCCAAATGTTGGGGGTCTATTATTCTAGACCATCCGGCAACGTTTGAGACGGTGGCCATGGACCCCGACTTGAAGCAGTGGATAATCGACGATTTAGATAGGTTTCTTCGAAGGAAAGATTTCTATAAGAAAGTGGGGAAGGCTTGGAAGAGGGGATATCTTCTGTATGGTCCTCCCGGTACGGGAAAATCAAGCTTAATCGCCGCCATAGCTAACTACCTCAAGTTCGATATCTATGATTTGGATCTCGCGAGCGTTTACAATAATACCATTCTGAGGAGGCTTTTGCTCGCCACCAAGAATCGGTCCATTTTGGTGATTGAAGATATAGATTGCAGCGCCAATCTACAAAATCGGCAGAGTGAGGAACATTTTGATGCATCCAACAGCAACAGCAAGCTTACCTTGTCGGGGATGCTTAATTTCATCGACGGATTGTGGTCGAGTTGTGGGGACGAAAGAATCATCATCTTCACGACGAACCACAAGGAGCGATTGGATCCGGCTCTGCTTCGGCCTGGTCGGATGGATGTTCATATAAACATGTCGTATTGTAGCCCTGAAGGGTTCAAGGTTCTGGCCTCTAATTACCTCGGCGGCGAATCGGTTCAGCATCGGCTGTACGGAGAAATCGAAGAGCTAATCAGAGATATGGAAGTATCTCCGGCGGAGATGGCGGAAGAGCTGATGAAGAGCGAGGAGGCCGATGCCGTTCTGGAAGGGCTCGTTGTTTTTCTGAAACGTAAACGGGAAGAACAGAGGAAGGCGAAAGAGGAGAAGGAAAGTGAGAAGAATGAAGAGAGAGAGGAACGAGAAGAAATGGACAAAACTGAAACTGAAGAAGAAGAAGAAGAAGAAGAAGAAAAAGAAAAAGAAGAAGAAGAACCCAAAAAATTAAAATGGGATATGAGAATTAGAAGGACGGGAAGGGGCCGGAGAATTTGA

Protein sequence

MFTFKEMVVPQSASAVFSAYASFATTIMLIRSITNELLPAQFLSFFSTIFFYFLGSSISSHTKFVIDENSGLFHLNQVFQAAEIYLRTKISPSIQTLRVTKTPQQNKIMLTIDKDQEIIDYFNNIRLQWRFVCLRDERDVSNPEKQYFELLFPTKFRDTVVDFYLPYVLRRAEEIREEDKVVKIYSQQCEVYDYSNPGPKCWGSIILDHPATFETVAMDPDLKQWIIDDLDRFLRRKDFYKKVGKAWKRGYLLYGPPGTGKSSLIAAIANYLKFDIYDLDLASVYNNTILRRLLLATKNRSILVIEDIDCSANLQNRQSEEHFDASNSNSKLTLSGMLNFIDGLWSSCGDERIIIFTTNHKERLDPALLRPGRMDVHINMSYCSPEGFKVLASNYLGGESVQHRLYGEIEELIRDMEVSPAEMAEELMKSEEADAVLEGLVVFLKRKREEQRKAKEEKESEKNEEREEREEMDKTETEEEEEEEEEKEKEEEEPKKLKWDMRIRRTGRGRRI
Homology
BLAST of Spg012691 vs. NCBI nr
Match: XP_022132942.1 (AAA-ATPase At2g18193-like, partial [Momordica charantia])

HSP 1 Score: 715.3 bits (1845), Expect = 3.7e-202
Identity = 384/511 (75.15%), Postives = 444/511 (86.89%), Query Frame = 0

Query: 1   MFTFKEMVVPQSASAVFSAYASFATTIMLIRSITNELLPAQFLSFFSTIFFYFLGSSISS 60
           M  FKEM +PQSASA FSAYASFATT+ML+RS+TNELLP +F+SF S+IFFYF G S+SS
Sbjct: 1   MLNFKEMPIPQSASAAFSAYASFATTMMLVRSVTNELLPPKFISFLSSIFFYFFG-SVSS 60

Query: 61  HTKFVIDENSGLFHLNQVFQAAEIYLRTKISPSIQTLRVTKTPQQNKIMLTIDKDQEIID 120
            TKFVI+E+SG F  NQVFQAAE+YLRTKISPS  TL+V+KTP+Q K+ L+IDKDQEI D
Sbjct: 61  QTKFVIEESSG-FTFNQVFQAAEVYLRTKISPSTDTLKVSKTPRQKKVTLSIDKDQEITD 120

Query: 121 YFNNIRLQWRFVCLRDERDVSNPEKQYFELLFPTKFRDTVVDFYLPYVLRRAEEIREEDK 180
           YF N RLQWRFVC +DER+ ++ EK++FEL+FP KFRD VVDFYLPYVLRRA+EI+EEDK
Sbjct: 121 YFENFRLQWRFVCSQDERNGNSREKRHFELIFPKKFRDRVVDFYLPYVLRRAKEIKEEDK 180

Query: 181 VVKIYSQQCEVYDYSNPGPKCWGSIILDHPATFETVAMDPDLKQWIIDDLDRFLRRKDFY 240
           VVKIYSQ+C+  D    G   WGSI LDHPATF+TVAM+P+LKQ+IIDDLDRF+RR+DFY
Sbjct: 181 VVKIYSQECQFDDDGGGGN--WGSINLDHPATFDTVAMEPELKQFIIDDLDRFVRRRDFY 240

Query: 241 KKVGKAWKRGYLLYGPPGTGKSSLIAAIANYLKFDIYDLDLASVYNNTILRRLLLATKNR 300
           KKVGKAWKRGYLLYGPPGTGKSSLIAA+ANYLKFDIYDLDL+++Y+N  LRR++LAT NR
Sbjct: 241 KKVGKAWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLSNIYSNADLRRVMLATTNR 300

Query: 301 SILVIEDIDCSANLQNRQSEEHFDASNSNSKLTLSGMLNFIDGLWSSCGDERIIIFTTNH 360
           SILVIEDIDCS ++QNRQ+EEHFD   S+SK TLSGMLNFIDGLWSSCGDERIIIFTTNH
Sbjct: 301 SILVIEDIDCSVDIQNRQNEEHFD--RSSSKFTLSGMLNFIDGLWSSCGDERIIIFTTNH 360

Query: 361 KERLDPALLRPGRMDVHINMSYCSPEGFKVLASNYLGGESVQHRLYGEIEELIRDMEVSP 420
           K+RLD ALLRPGRMDVHINMSYCSP GF+VLASNYLG E+ QHRLYGEIE+LIRDMEVSP
Sbjct: 361 KDRLDSALLRPGRMDVHINMSYCSPHGFRVLASNYLGEEATQHRLYGEIEDLIRDMEVSP 420

Query: 421 AEMAEELMKSEEADAVLEGLVVFLKRKREEQRKAKEEKESEKNEEREEREEMDKTETEEE 480
           AE+AEELMKS++ADAVL+GLV FLKRK+EEQRKAKEEK SEKNEE+EE+        E +
Sbjct: 421 AEIAEELMKSDDADAVLQGLVEFLKRKKEEQRKAKEEKASEKNEEKEEK--------EGD 480

Query: 481 EEEEEEKEKEEEEPKKLKWDMRIRRTGRGRR 512
           E EEEE+ + E EPK+L+   R RRTG G R
Sbjct: 481 EIEEEEEPEPEPEPKRLR-IRRPRRTGYGYR 496

BLAST of Spg012691 vs. NCBI nr
Match: XP_038877872.1 (AAA-ATPase At2g18193-like [Benincasa hispida])

HSP 1 Score: 687.6 bits (1773), Expect = 8.4e-194
Identity = 378/511 (73.97%), Postives = 434/511 (84.93%), Query Frame = 0

Query: 1   MFTFKEMVVPQSASAVFSAYASFATTIMLIRSITNELLPAQFLSFFSTIFFYFLGSSISS 60
           MF FKEM VPQSASAVFSAYASFATT+MLIRS+TNELLPA+F+SF S+IF YF G SI+S
Sbjct: 1   MFNFKEMAVPQSASAVFSAYASFATTMMLIRSVTNELLPAKFISFLSSIFVYFFG-SIAS 60

Query: 61  HTKFVIDENSGLFHLNQVFQAAEIYLRTKISPSIQTLRVTKTPQQNKIMLTIDKDQEIID 120
            TKFVI+ENSG F +N+VFQAAE YLRTKISPSI TL+V+KTP+Q K+ L+IDKDQEIID
Sbjct: 61  QTKFVIEENSG-FTINEVFQAAEFYLRTKISPSIDTLKVSKTPRQKKVRLSIDKDQEIID 120

Query: 121 YFNNIRLQWRFVCLRDERD-VSNPEKQYFELLFPTKFRDTVVDFYLPYVLRRAEEIREED 180
           YF NIRLQWRFVC  DER+     EK++FEL FP KFRD VVDFYLPYVL+RA+EI+EE 
Sbjct: 121 YFENIRLQWRFVCSVDERNGGGGREKRHFELSFPKKFRDRVVDFYLPYVLKRAKEIKEEH 180

Query: 181 KVVKIYSQQCEVYDYSNPGPKCWGSIILDHPATFETVAMDPDLKQWIIDDLDRFLRRKDF 240
           KVVKI+SQ+C+ YD  + G   WGS+ L+HPATF+T+AMDP+LKQ IIDDL+RF+RRKDF
Sbjct: 181 KVVKIFSQECQ-YDDDSGGN--WGSVNLEHPATFDTLAMDPELKQSIIDDLNRFVRRKDF 240

Query: 241 YKKVGKAWKRGYLLYGPPGTGKSSLIAAIANYLKFDIYDLDLASVYNNTILRRLLLATKN 300
           YKKVGKAWKRGYLLYGPPGTGKSSLIAA+ANYLKFDIYDLDL ++Y+N+ LRR+LLAT N
Sbjct: 241 YKKVGKAWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLTNMYSNSDLRRVLLATTN 300

Query: 301 RSILVIEDIDCSANLQNRQSEEHFDASNSNSKLTLSGMLNFIDGLWSSCGDERIIIFTTN 360
           RSILVIEDIDCS  +QNRQSEEHFD   SNSK TLSGMLNFIDGLWSSCGDERIIIFTTN
Sbjct: 301 RSILVIEDIDCSVEIQNRQSEEHFD--RSNSKFTLSGMLNFIDGLWSSCGDERIIIFTTN 360

Query: 361 HKERLDPALLRPGRMDVHINMSYCSPEGFKVLASNYLGGESVQHRLYGEIEELIRDMEVS 420
           HK+RLDPALLRPGRMDVHINMSYCS EG KVL SNYLGGE+ +H +YGEIEEL+ DMEVS
Sbjct: 361 HKDRLDPALLRPGRMDVHINMSYCSREGLKVLVSNYLGGEATKHCMYGEIEELMEDMEVS 420

Query: 421 PAEMAEELMKSEEADAVLEGLVVFLKRKREEQRKAKEEKESEKNEEREEREEMDKTETEE 480
           PAE+AEELMK EE +AVL GLV FLKRKREEQRK KEEK  E+ EER+E EE++    E 
Sbjct: 421 PAEIAEELMKGEETEAVLGGLVGFLKRKREEQRKEKEEKNEEEEEERDEIEEVE----EG 480

Query: 481 EEEEEEEKEKEEEEPKKLKWDMRIRRTGRGR 511
            ++E+ E++K E   + L+     R  GRGR
Sbjct: 481 GDDEDSERKKWEPRNRMLRIGYGYRGRGRGR 500

BLAST of Spg012691 vs. NCBI nr
Match: XP_008440759.1 (PREDICTED: AAA-ATPase At2g18193-like [Cucumis melo])

HSP 1 Score: 678.3 bits (1749), Expect = 5.1e-191
Identity = 373/511 (72.99%), Postives = 433/511 (84.74%), Query Frame = 0

Query: 4   FKEMVVPQSASAVFSAYASFATTIMLIRSITNELLPAQFLSFFSTIFFYFLGSSISSHTK 63
           FK+M VPQS SAVFSAYASFATT+MLIRS+TNELLPA+ +SF S+IF YF G SISS TK
Sbjct: 3   FKDMAVPQSVSAVFSAYASFATTMMLIRSVTNELLPAKLISFLSSIFVYFFG-SISSQTK 62

Query: 64  FVIDENSGLFHLNQVFQAAEIYLRTKISPSIQTLRVTKTPQQNKIMLTIDKDQEIIDYFN 123
           FVI+ENSG F +N+VFQAAE YLRTKISPSI TL+VTKTP+Q K+ L+IDKDQEIIDYF 
Sbjct: 63  FVIEENSG-FAMNEVFQAAEFYLRTKISPSIDTLKVTKTPRQKKVTLSIDKDQEIIDYFE 122

Query: 124 NIRLQWRFVCLRDERD-VSNPEKQYFELLFPTKFRDTVVDFYLPYVLRRAEEIREEDKVV 183
           NIRLQWRF+C  DER+     EK+ FELLFP KFRD VVDFYLPYVL+RA+EI+EE+KVV
Sbjct: 123 NIRLQWRFLCSVDERNGGGGREKRQFELLFPKKFRDRVVDFYLPYVLKRAKEIKEENKVV 182

Query: 184 KIYSQQCEVYDYSNPGPKCWGSIILDHPATFETVAMDPDLKQWIIDDLDRFLRRKDFYKK 243
           KI+SQ+C+ YD  + G   WGS+ L+HPATF+T+AMDP+LKQ II+DLDRF+RRKDFYKK
Sbjct: 183 KIFSQECQ-YDDDSGGN--WGSVNLEHPATFDTLAMDPELKQSIIEDLDRFVRRKDFYKK 242

Query: 244 VGKAWKRGYLLYGPPGTGKSSLIAAIANYLKFDIYDLDLASVYNNTILRRLLLATKNRSI 303
           VGKAWKRGYLLYGPPGTGKSSLIAA+ANYLKFDIYDLDL+++Y+N+ LRR+LLAT NRSI
Sbjct: 243 VGKAWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLSNMYSNSDLRRVLLATTNRSI 302

Query: 304 LVIEDIDCSANLQNRQSEEHFDASNSNSKLTLSGMLNFIDGLWSSCGDERIIIFTTNHKE 363
           LVIEDIDCS  +QNRQSEE+FD   S+SK TLSGMLNFIDGLWSSCGDERIIIFTTN+K 
Sbjct: 303 LVIEDIDCSVEIQNRQSEENFD--RSSSKFTLSGMLNFIDGLWSSCGDERIIIFTTNNKH 362

Query: 364 RLDPALLRPGRMDVHINMSYCSPEGFKVLASNYLGGESVQHRLYGEIEELIRDMEVSPAE 423
           RLDPALLR GRMD+HINMSYCS EG KVL SNYLGGE+ +H  YGEIEELI +MEV+PAE
Sbjct: 363 RLDPALLRAGRMDMHINMSYCSREGLKVLVSNYLGGEATKHSTYGEIEELIGEMEVAPAE 422

Query: 424 MAEELMKSEEADAVLEGLVVFLKRKREEQRKAKEEKESEKNEEREEREEMDKTETEEEEE 483
           +AEELMK EE +AVL GL+ FLKRKREEQRK KEEK+ EK EE ++          EEEE
Sbjct: 423 IAEELMKGEETEAVLGGLLGFLKRKREEQRKEKEEKKEEKGEEEDK---------IEEEE 482

Query: 484 EEEEKEKEEEEPKKLKWDM--RIRRTGRGRR 512
           E ++ E+EEE  KK+KW++  R+RR G G R
Sbjct: 483 EGDKIEEEEEATKKIKWELRNRVRRIGYGYR 497

BLAST of Spg012691 vs. NCBI nr
Match: XP_004149988.3 (AAA-ATPase At2g18193 [Cucumis sativus] >KGN49285.2 hypothetical protein Csa_003255 [Cucumis sativus])

HSP 1 Score: 671.8 bits (1732), Expect = 4.8e-189
Identity = 371/511 (72.60%), Postives = 430/511 (84.15%), Query Frame = 0

Query: 4   FKEMVVPQSASAVFSAYASFATTIMLIRSITNELLPAQFLSFFSTIFFYFLGSSISSHTK 63
           FK+M VPQS SA+FSAYASFATT+MLIRS+TNELLPA+ +S FS+IF YF G SISS TK
Sbjct: 3   FKDMAVPQSVSALFSAYASFATTMMLIRSLTNELLPAKLIS-FSSIFVYFFG-SISSQTK 62

Query: 64  FVIDENSGLFHLNQVFQAAEIYLRTKISPSIQTLRVTKTPQQNKIMLTIDKDQEIIDYFN 123
            VI+ENSG F +N+VFQAAE YLRTKISPSI TL+VTKTP+Q K+ L+IDKDQEIIDYF 
Sbjct: 63  LVIEENSG-FAMNEVFQAAEFYLRTKISPSIDTLKVTKTPRQKKVTLSIDKDQEIIDYFE 122

Query: 124 NIRLQWRFVCLRDERD-VSNPEKQYFELLFPTKFRDTVVDFYLPYVLRRAEEIREEDKVV 183
           NIRLQWRF+C  DER+   + EK+ FEL FP KFRD +VDFYLPYVLRRA+EI+EE+KVV
Sbjct: 123 NIRLQWRFLCSVDERNGGGSREKRQFELSFPKKFRDRIVDFYLPYVLRRAKEIKEENKVV 182

Query: 184 KIYSQQCEVYDYSNPGPKCWGSIILDHPATFETVAMDPDLKQWIIDDLDRFLRRKDFYKK 243
           KI+SQ+C+ YD  + G   WGS+ L+HPATF+T+AMDP+LKQ II+DLDRF+RRKDFYKK
Sbjct: 183 KIFSQECQ-YDDDSGGN--WGSVNLEHPATFDTLAMDPELKQSIIEDLDRFVRRKDFYKK 242

Query: 244 VGKAWKRGYLLYGPPGTGKSSLIAAIANYLKFDIYDLDLASVYNNTILRRLLLATKNRSI 303
           VGKAWKRGYLLYGPPGTGKSSLIAA+ANYLKFDIYDLDL ++Y+N+ LRR+LLAT NRSI
Sbjct: 243 VGKAWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLTNMYSNSDLRRVLLATTNRSI 302

Query: 304 LVIEDIDCSANLQNRQSEEHFDASNSNSKLTLSGMLNFIDGLWSSCGDERIIIFTTNHKE 363
           LVIEDIDCS  +QNRQSEEHFD   S+SK TLSGMLNFIDGLWSSCGDERIIIFTTN+K 
Sbjct: 303 LVIEDIDCSVQIQNRQSEEHFD--QSSSKFTLSGMLNFIDGLWSSCGDERIIIFTTNNKH 362

Query: 364 RLDPALLRPGRMDVHINMSYCSPEGFKVLASNYLGGESVQHRLYGEIEELIRDMEVSPAE 423
           RLDPALLR GRMD+HINMSYCS EG +VL SNYLGGE+ +H  YGEIEELI +MEV+PAE
Sbjct: 363 RLDPALLRAGRMDMHINMSYCSREGLRVLVSNYLGGEATKHSTYGEIEELIGEMEVAPAE 422

Query: 424 MAEELMKSEEADAVLEGLVVFLKRKREEQRKAKEEKESEKNEEREEREEMDKTETEEEEE 483
           +AEELMK EE +AVL GLV FLKRKREE+RK KEEK+ EK EE E        + EEEEE
Sbjct: 423 IAEELMKGEETEAVLGGLVGFLKRKREEERKEKEEKKEEKGEEEE--------KVEEEEE 482

Query: 484 EEEEKEKEEEEPKKLKWDM--RIRRTGRGRR 512
           E ++  +EEE  KK  W++  R+RRTG G R
Sbjct: 483 EGDKIVEEEEATKKTNWELRNRVRRTGYGYR 497

BLAST of Spg012691 vs. NCBI nr
Match: XP_038877870.1 (AAA-ATPase At2g18193-like [Benincasa hispida])

HSP 1 Score: 655.6 bits (1690), Expect = 3.5e-184
Identity = 347/480 (72.29%), Postives = 404/480 (84.17%), Query Frame = 0

Query: 1   MFTFKEMVVPQSASAVFSAYASFATTIMLIRSITNELLPAQFLSFFSTIFFYFLGSSISS 60
           M   KEMVVPQS SAVFSAYASFATT+ML RS+TN+LLPA+F+SF S+I+ YF G S+SS
Sbjct: 1   MINLKEMVVPQSVSAVFSAYASFATTMMLFRSVTNDLLPAKFISFLSSIYVYFFG-SVSS 60

Query: 61  HTKFVIDENSGLFHLNQVFQAAEIYLRTKISPSIQTLRVTKTPQQNKIMLTIDKDQEIID 120
            TKFVIDENSG+  +N+VFQAAEIYLRT+I+PSI  L+V K+P+Q  I L+I KDQEI D
Sbjct: 61  QTKFVIDENSGI-TVNEVFQAAEIYLRTRINPSIDILKVHKSPRQKNITLSIYKDQEITD 120

Query: 121 YFNNIRLQWRFVCLRDERDVSNPEKQYFELLFPTKFRDTVVDFYLPYVLRRAEEIREEDK 180
           YF NIRLQWR VC  D+ + +N E ++FELLFP KFRD VVD YLPYVL RA+E+ EE+K
Sbjct: 121 YFQNIRLQWRLVCFADQHN-ANTEIRHFELLFPKKFRDRVVDSYLPYVLWRAKELEEENK 180

Query: 181 VVKIYSQQCEVYDYSNPGPKCWGSIILDHPATFETVAMDPDLKQWIIDDLDRFLRRKDFY 240
           VVKI+SQ+C+  DY+  G   WGS+ LD PATF+T+AM+P+LKQWIIDDLDRF+RRKDFY
Sbjct: 181 VVKIFSQECQYDDYAGYGK--WGSVNLDRPATFDTLAMEPELKQWIIDDLDRFVRRKDFY 240

Query: 241 KKVGKAWKRGYLLYGPPGTGKSSLIAAIANYLKFDIYDLDLASVYNNTILRRLLLATKNR 300
           + VGK WKRGYLLYGPPGTGKSSLIAAIANYL+FDIYDLDL+++Y+ + LRR+LLATKNR
Sbjct: 241 RNVGKTWKRGYLLYGPPGTGKSSLIAAIANYLRFDIYDLDLSNMYSYSDLRRILLATKNR 300

Query: 301 SILVIEDIDCSANLQNRQSEEHFDASNSNSKLTLSGMLNFIDGLWSSCGDERIIIFTTNH 360
           SILVIEDIDCS  +QNR+S +HFD   SN K TLSGMLNFIDGLWSSCGDERII+FTTNH
Sbjct: 301 SILVIEDIDCSVKIQNRESGKHFD--QSNGKFTLSGMLNFIDGLWSSCGDERIIMFTTNH 360

Query: 361 KERLDPALLRPGRMDVHINMSYCSPEGFKVLASNYLGGESVQHRLYGEIEELIRDMEVSP 420
           KERLDPALLRPGRMDVHINMSYCS +GFKVLASNYLGGE+ +HR+Y EIEELI DMEVSP
Sbjct: 361 KERLDPALLRPGRMDVHINMSYCSRQGFKVLASNYLGGEATEHRVYREIEELIGDMEVSP 420

Query: 421 AEMAEELMKSEEADAVLEGLVVFLKRKREEQRKAKEEKESEKNEEREEREEMDKTETEEE 480
           AE+ EELMK EEA+ VL GLV FLKRK+EEQRK KE K        E+++E DK E EEE
Sbjct: 421 AEIGEELMKGEEAEGVLGGLVDFLKRKKEEQRKEKEVK------NEEQKKEWDKIEEEEE 467

BLAST of Spg012691 vs. ExPASy Swiss-Prot
Match: Q8GW96 (AAA-ATPase At2g18193 OS=Arabidopsis thaliana OX=3702 GN=At2g18193 PE=2 SV=1)

HSP 1 Score: 433.3 bits (1113), Expect = 3.7e-120
Identity = 247/487 (50.72%), Postives = 351/487 (72.07%), Query Frame = 0

Query: 12  SASAVFSAYASFATTIMLIRSITNELLPAQFLSFFSTIFFYFLGSSISSHTKFVIDENSG 71
           S S++FSAYAS    +ML RS+ ++ +P +  S+FS++   F  +  S +   +IDEN G
Sbjct: 10  SPSSLFSAYASLTGFLMLFRSMLHDFVPEKLRSYFSSLLDRFF-TPKSKYLTVIIDENFG 69

Query: 72  LFHLNQVFQAAEIYLRTKISPSIQTLRVTKTPQQNKIMLTIDKDQEIIDYFNNIRLQWRF 131
           L + NQVF AAE+YLR+KI P  + LRV K P+Q    ++I++ +EI+D F    ++W +
Sbjct: 70  L-NRNQVFDAAEMYLRSKIGPETERLRVGKIPKQKHFTISIERGEEILDTFEESEVKWSY 129

Query: 132 VCLRDERDVSNPEKQYFELLFPTKFRDTVVDFYLPYVLRRAEEIREEDKVVKIYSQQCEV 191
           V  + E +  +  K+Y+EL F  K RD V++ YL +V+  +EEI+   +VVK+YS+  +V
Sbjct: 130 V--QSENEKGDKVKRYYELTFEKKLRDKVLNSYLTHVVAESEEIKRNLRVVKLYSR--DV 189

Query: 192 Y---DYSNPGPKCWGSIILDHPATFETVAMDPDLKQWIIDDLDRFLRRKDFYKKVGKAWK 251
           Y   D        WG I L+HP+TF+T+AMDP+ K+ IIDDL+RFL+RK+FYK+VGKAWK
Sbjct: 190 YASDDDDGMAGGNWGCINLEHPSTFDTLAMDPNAKKKIIDDLERFLKRKEFYKRVGKAWK 249

Query: 252 RGYLLYGPPGTGKSSLIAAIANYLKFDIYDLDLASVYNNTILRRLLLATKNRSILVIEDI 311
           RGYLLYGPPGTGKSSLIAA+ANYLKFD++DL+L+S+Y+N  L+R+LL+T NRSILVIEDI
Sbjct: 250 RGYLLYGPPGTGKSSLIAAMANYLKFDVFDLELSSIYDNGELKRVLLSTTNRSILVIEDI 309

Query: 312 DCSANLQNRQSEEHFDASNSNSKLTLSGMLNFIDGLWSSCGDERIIIFTTNHKERLDPAL 371
           DC+A +++R++E   D      K+TLSG+LNFIDGLWSS GDERII+FTTNHKERLDPAL
Sbjct: 310 DCNAEVRDREAENQED-EQIKGKVTLSGILNFIDGLWSSFGDERIIVFTTNHKERLDPAL 369

Query: 372 LRPGRMDVHINMSYCSPEGFKVLASNYLGGESVQHRLYGEIEELIRDMEVSPAEMAEELM 431
           LRPGRMDVHINMSYC+  GF+ L SNYLG + + H L  EIE L+   EV+PAE+AEELM
Sbjct: 370 LRPGRMDVHINMSYCTGLGFRTLVSNYLGLDGLNHPLCEEIEALVDSTEVTPAELAEELM 429

Query: 432 KSEEADAVLEGLVVFLKRKREEQRKAKEEKESEKNEEREEREEMDKTETEEEEEEEEEKE 491
           + ++ D VL G++ F+++++ E+ K K+E    K  + +E++       +++++  ++K 
Sbjct: 430 QDDDTDVVLRGVISFVEKRKVERSKTKKEVSICKATDDDEKQNGSLGCVKKKKKGGKQKG 489

Query: 492 KEEEEPK 496
           K + + K
Sbjct: 490 KGKGKGK 489

BLAST of Spg012691 vs. ExPASy Swiss-Prot
Match: Q147F9 (AAA-ATPase At3g50940 OS=Arabidopsis thaliana OX=3702 GN=At3g50940 PE=2 SV=1)

HSP 1 Score: 432.6 bits (1111), Expect = 6.4e-120
Identity = 230/448 (51.34%), Postives = 320/448 (71.43%), Query Frame = 0

Query: 12  SASAVFSAYASFATTIMLIRSITNELLPAQFLSFFSTIFFYFLGSSISSHTKFVIDENSG 71
           +A    +A AS A   +L RS+  + +P +   + S  F  F  S  S     VI+E  G
Sbjct: 11  TAKTALTAVASVAAAAILARSVVQDYMPNEVHEYISHGFRRFF-SYFSYQMTAVIEEFGG 70

Query: 72  LFHLNQVFQAAEIYLRTKISPSIQTLRVTKTPQQNKIMLTIDKDQEIIDYFNNIRLQWRF 131
             H NQVF+AAE YL TKIS S + ++V K  +Q+   +T+++D+E++D F+ ++L W  
Sbjct: 71  FEH-NQVFEAAEAYLSTKISNSTRRIKVNKLEKQSNYSVTVERDEEVVDIFDGVKLSWIL 130

Query: 132 VCLR-DERDVSNP---------EKQYFELLFPTKFRDTVVDFYLPYVLRRAEEIREEDKV 191
           VC   D++D  NP         E + +EL F  KF++ V++ YLP+V+ +A  I+++ K 
Sbjct: 131 VCRHVDKKDFRNPRDLNSTLKSEVRSYELSFRKKFKNMVLESYLPFVVEQAASIKQKFKT 190

Query: 192 VKIYSQQCEVYDYSNPGPKCWGSIILDHPATFETVAMDPDLKQWIIDDLDRFLRRKDFYK 251
           +KI++    V  YS      W S+ LDHP+TF T+A+DP++K+ +++DLDRF++RK FY 
Sbjct: 191 LKIFT----VDSYSVE----WTSVTLDHPSTFRTLALDPEVKKNLVEDLDRFVQRKGFYG 250

Query: 252 KVGKAWKRGYLLYGPPGTGKSSLIAAIANYLKFDIYDLDLASVYNNTILRRLLLATKNRS 311
           +VGKAWKRGYLLYGPPGTGKSSLIAAIAN+L FDIYDLDL S+ NN  LRRLL++T NRS
Sbjct: 251 RVGKAWKRGYLLYGPPGTGKSSLIAAIANHLNFDIYDLDLTSLNNNAELRRLLMSTANRS 310

Query: 312 ILVIEDIDCSANLQNRQSEEHFDASNSNSKLTLSGMLNFIDGLWSSCGDERIIIFTTNHK 371
           ILV+EDIDCS  L++R +++  +    +  +TLSG+LNF+DGLWSSCG+ERII+FTTN++
Sbjct: 311 ILVVEDIDCSIELKDRSTDQE-NNDPLHKTVTLSGLLNFVDGLWSSCGNERIIVFTTNYR 370

Query: 372 ERLDPALLRPGRMDVHINMSYCSPEGFKVLASNYLGGESVQHRLYGEIEELIRDMEVSPA 431
           E+LDPALLRPGRMD+HI+MSYC+P  FKVLASNYL  E   H L+ +IEE IR++EV+PA
Sbjct: 371 EKLDPALLRPGRMDMHIHMSYCTPAAFKVLASNYL--EIQDHILFEQIEEFIREIEVTPA 430

Query: 432 EMAEELMKSEEADAVLEGLVVFLKRKRE 450
           E+AE+LM+S+  D VL+GLV FLK K++
Sbjct: 431 EVAEQLMRSDSVDKVLQGLVEFLKAKKQ 445

BLAST of Spg012691 vs. ExPASy Swiss-Prot
Match: Q8VZG2 (Protein HYPER-SENSITIVITY-RELATED 4 OS=Arabidopsis thaliana OX=3702 GN=HSR4 PE=2 SV=1)

HSP 1 Score: 418.7 bits (1075), Expect = 9.5e-116
Identity = 250/503 (49.70%), Postives = 350/503 (69.58%), Query Frame = 0

Query: 12  SASAVFSAYASFATTIMLIRSITNELLPAQ---FLSF-FSTIFFYFLGSSISSHTKFVID 71
           +A  V +  AS A T ML RS+  + LP +   ++S+ F +IF YF     SS    +I+
Sbjct: 57  TAKTVLTTAASVAATAMLARSLVQDYLPDEVHHYISYGFRSIFGYF-----SSQMTIIIE 116

Query: 72  ENSGLFHLNQVFQAAEIYLRTKISPSIQTLRVTKTPQQNKIMLTIDKDQEIIDYFNNIRL 131
           E  G  H N+VF+AAE YL TKISPS + ++V+K  ++N   +T+++D+E++D +N ++ 
Sbjct: 117 EFEGFAH-NEVFEAAEAYLATKISPSNKRIKVSKHEKENNYNVTVERDEEVVDTYNGVKF 176

Query: 132 QWRFVCL----------RDERDVSNPEKQYFELLFPTKFRDTVVDFYLPYVLRRAEEIRE 191
           QW   C           RD       E + FEL F  KF+D  ++ YLP++++RA  +++
Sbjct: 177 QWILHCRHVESKHFHNPRDLNSTLRSEVRSFELNFHKKFKDVALESYLPFMVKRATLMKQ 236

Query: 192 EDKVVKIYSQQCE-VY-DYSNPGPKCWGSIILDHPATFETVAMDPDLKQWIIDDLDRFLR 251
           E K +KI++   E +Y +YS+     W S+ LDHP+TF+T+AMD D+K  +++DLD+F++
Sbjct: 237 EKKTLKIFTLSPENMYGNYSD----AWTSVTLDHPSTFKTLAMDSDVKTSVMEDLDKFVK 296

Query: 252 RKDFYKKVGKAWKRGYLLYGPPGTGKSSLIAAIANYLKFDIYDLDLASVYNNTILRRLLL 311
           R+DFYK+VGKAWKRGYLLYGPPGTGKSSLIAA+AN+L FDIYDL+L +V NN+ LRRLL+
Sbjct: 297 RRDFYKRVGKAWKRGYLLYGPPGTGKSSLIAAMANHLNFDIYDLELTAVNNNSELRRLLI 356

Query: 312 ATKNRSILVIEDIDCSANLQNRQSEEHFDASNS-----NSKLTLSGMLNFIDGLWSSCGD 371
           AT NRSIL++EDIDCS  L++R S+E    S+        K+TLSG+LNFIDGLWSSCGD
Sbjct: 357 ATANRSILIVEDIDCSLELKDRTSDEPPRESDDIEDPRYKKVTLSGLLNFIDGLWSSCGD 416

Query: 372 ERIIIFTTNHKERLDPALLRPGRMDVHINMSYCSPEGFKVLASNYLGGESVQHRLYGEIE 431
           ERIIIFTTN+KE+LD ALLRPGRMD+HI+MSYC+P  FK LA NYL  E  +HRL+ +IE
Sbjct: 417 ERIIIFTTNYKEKLDAALLRPGRMDMHIHMSYCTPSTFKALALNYL--EIKEHRLFSKIE 476

Query: 432 ELIRDMEVSPAEMAEELMKSEEADAVLEGLVVFLKRKR--EEQRKAKEEKESEKNEE--R 490
           E I   EV+PAE+AE+LM+++  D VLEGL+ FLK K+   EQ KAK EK+  +N++  +
Sbjct: 477 EGIEATEVTPAEVAEQLMRNDSVDKVLEGLIEFLKVKKIENEQDKAKTEKQELENKKKTK 536

BLAST of Spg012691 vs. ExPASy Swiss-Prot
Match: F4IQG2 (AAA-ATPase At2g18190 OS=Arabidopsis thaliana OX=3702 GN=At2g18190 PE=2 SV=1)

HSP 1 Score: 417.5 bits (1072), Expect = 2.1e-115
Identity = 241/482 (50.00%), Postives = 334/482 (69.29%), Query Frame = 0

Query: 12  SASAVFSAYASFATTIMLIRSITNELLPAQFLSFFSTIFFYFLGSSISSHTKFVIDENSG 71
           S S++F+AYAS    +ML RS+ N+ +P +  S+ + +   F  +  S +   VIDE  G
Sbjct: 11  SPSSLFTAYASLTGFLMLFRSLFNDEVPERLRSYITDLLNRFF-TPKSKNLTMVIDEIIG 70

Query: 72  LFHLNQVFQAAEIYLRTKISPSIQTLRVTKTPQQNKIMLTIDKDQEIIDYFNNIRLQWRF 131
            F  NQVF AAE+YLR KI P    LRV K P+Q    + I+K +EI+D F N  L+W +
Sbjct: 71  -FKRNQVFDAAEVYLRNKIGPETARLRVGKLPKQKHFTIYIEKGEEILDTFENSELRWTY 130

Query: 132 VCLRDERDVSNPEKQYFELLFPTKFRDTVVDFYLPYVLRRAEEIREEDKVVKIYSQQCEV 191
           V    E + S  EK+Y+EL F  K RD V++ YL +V+  +EE + + + VK+YS+    
Sbjct: 131 V--ESENEASQKEKRYYELTFEKKLRDKVMNSYLSHVVAESEETKRDLRAVKLYSRDVRA 190

Query: 192 -YDYSNPGPKCWGSIILDHPATFETVAMDPDLKQWIIDDLDRFLRRKDFYKKVGKAWKRG 251
             D        WG I L+HP+TFET+AMDP  K+ IIDD++RFL+R++FYK+VGKAWKRG
Sbjct: 191 SKDDDGMAGAGWGCINLEHPSTFETLAMDPGAKKKIIDDMERFLKRREFYKRVGKAWKRG 250

Query: 252 YLLYGPPGTGKSSLIAAIANYLKFDIYDLDLASVYNNTILRRLLLATKNRSILVIEDIDC 311
           YLLYGPPGTGKSSLIAA+ANYLKFD++DL+L+S+Y N  L+ +LL+T NRSILVIEDIDC
Sbjct: 251 YLLYGPPGTGKSSLIAAMANYLKFDVFDLELSSIYENAQLKSILLSTTNRSILVIEDIDC 310

Query: 312 -SANLQNRQSEEHFDASNS-NSKLTLSGMLNFIDGLWSSCGDERIIIFTTNHKERLDPAL 371
            SA + +R+++E+ +       ++TLSG+LNF+DGLWSS GDERII+FTTNHKERLDPAL
Sbjct: 311 SSAEVVDREADEYQEYEEGYYGRVTLSGLLNFVDGLWSSFGDERIIVFTTNHKERLDPAL 370

Query: 372 LRPGRMDVHINMSYCSPEGFKVLASNYLGGESVQHRLYGEIEELIRDMEVSPAEMAEELM 431
           LRPGRMD+HINMSYC+  GF+ L SNYLG   + H L  EIE LI   EV+PAE+AEELM
Sbjct: 371 LRPGRMDMHINMSYCTGLGFRTLVSNYLGLGGLNHPLCEEIEALIDSTEVTPAELAEELM 430

Query: 432 KSEEADAVLEGLVVFLKRKREEQRKAKE-EKESEKNEEREEREEMDKTETEEEEEEEEEK 490
           + ++ D VL G+V F++ ++ E  K KE E  + +  + +++  +  T   ++ +++++ 
Sbjct: 431 QEDDTDVVLRGVVSFVENRKVEISKTKELEGSTCRKLDGDDKHNVSSTNDLKKTKKKKKG 488

BLAST of Spg012691 vs. ExPASy Swiss-Prot
Match: Q9FN75 (AAA-ATPase At5g17760 OS=Arabidopsis thaliana OX=3702 GN=At5g17760 PE=3 SV=1)

HSP 1 Score: 412.1 bits (1058), Expect = 8.9e-114
Identity = 252/515 (48.93%), Postives = 347/515 (67.38%), Query Frame = 0

Query: 1   MFTFKEMVVPQSASAVFSAYASFATTIMLIRSITNELLPAQFLSF-FSTIFFYFLGSSIS 60
           MF  K++  P S   VF+AYAS A  +M+IRS+ +EL+PA    F + T+   F  SS S
Sbjct: 1   MFFSKDLPSPTS---VFTAYASMAGYMMMIRSMAHELIPAPLQDFIYRTLRSLFFRSSSS 60

Query: 61  SHTKFVIDENSGLFHLNQVFQAAEIYLRTKISPSIQTLRVTKTPQQNKIMLTIDKDQEII 120
           + T  + D+N G+   N++++AA+ YL TKISP    LR++K  +   + L +   + + 
Sbjct: 61  TLTLTIDDDNMGM--NNEIYRAAQTYLSTKISPDAVRLRISKGHKDKHVNLYLSDGEIVN 120

Query: 121 DYFNNIRLQWRFVCLRDERD-------------------VSNPEKQYFELLFPTKFRDTV 180
           D + +++L WRFV    ++                      + + +YFEL F  K +D +
Sbjct: 121 DVYEDVQLVWRFVTDGGDKKGGGGGVGGRGGGGGRRGGMDDDGKSEYFELSFDKKHKDLI 180

Query: 181 VDFYLPYVLRRAEEIREEDKVVKIYSQQCEVYDYSNPGPKCWGSIILDHPATFETVAMDP 240
           ++ Y+PY+  +A+EIR+E +++ ++S               W S+IL+HP+TFET+AM+ 
Sbjct: 181 LNSYVPYIESKAKEIRDERRILMLHSL----------NSLRWESVILEHPSTFETMAMED 240

Query: 241 DLKQWIIDDLDRFLRRKDFYKKVGKAWKRGYLLYGPPGTGKSSLIAAIANYLKFDIYDLD 300
           DLK+ +I+DLDRF+RRK+FYK+VGKAWKRGYLLYGPPGTGKSSL+AA+ANYLKFD+YDL 
Sbjct: 241 DLKRDVIEDLDRFIRRKEFYKRVGKAWKRGYLLYGPPGTGKSSLVAAMANYLKFDVYDLQ 300

Query: 301 LASVYNNTILRRLLLATKNRSILVIEDIDCSANLQNR--QSEEHFDASNSNSKLTLSGML 360
           LASV  ++ LRRLLLAT+NRSILVIEDIDC+ +L NR  Q  E  +   S   LTLSG+L
Sbjct: 301 LASVMRDSDLRRLLLATRNRSILVIEDIDCAVDLPNRIEQPVEGKNRGESQGPLTLSGLL 360

Query: 361 NFIDGLWSSCGDERIIIFTTNHKERLDPALLRPGRMDVHINMSYCSPEGFKVLASNYLG- 420
           NFIDGLWSSCGDERIIIFTTNHK+RLDPALLRPGRMD+HI M +CS +GFK LASNYLG 
Sbjct: 361 NFIDGLWSSCGDERIIIFTTNHKDRLDPALLRPGRMDMHIYMGHCSFQGFKTLASNYLGL 420

Query: 421 -GESVQHRLYGEIEELIRDMEVSPAEMAEELMKSEEADAVLEGLVVFLKRKREEQRKAKE 480
              ++ HRL+ EIE LI    ++PA++AEELMKSE+AD  LEGLV  L++ R + +++  
Sbjct: 421 SDAAMPHRLFPEIERLIDGEVMTPAQVAEELMKSEDADVALEGLVNVLEKMRLKSKESNP 480

Query: 481 EKESEKNEEREEREEMD-KTETEEEEEEEEEKEKE 491
               +K E R E EEM  K++TE    +  ++ K+
Sbjct: 481 VMMKQK-ESRLEMEEMRLKSDTEGSPRKNSKRFKK 499

BLAST of Spg012691 vs. ExPASy TrEMBL
Match: A0A6J1BV86 (AAA-ATPase At2g18193-like OS=Momordica charantia OX=3673 GN=LOC111005662 PE=3 SV=1)

HSP 1 Score: 715.3 bits (1845), Expect = 1.8e-202
Identity = 384/511 (75.15%), Postives = 444/511 (86.89%), Query Frame = 0

Query: 1   MFTFKEMVVPQSASAVFSAYASFATTIMLIRSITNELLPAQFLSFFSTIFFYFLGSSISS 60
           M  FKEM +PQSASA FSAYASFATT+ML+RS+TNELLP +F+SF S+IFFYF G S+SS
Sbjct: 1   MLNFKEMPIPQSASAAFSAYASFATTMMLVRSVTNELLPPKFISFLSSIFFYFFG-SVSS 60

Query: 61  HTKFVIDENSGLFHLNQVFQAAEIYLRTKISPSIQTLRVTKTPQQNKIMLTIDKDQEIID 120
            TKFVI+E+SG F  NQVFQAAE+YLRTKISPS  TL+V+KTP+Q K+ L+IDKDQEI D
Sbjct: 61  QTKFVIEESSG-FTFNQVFQAAEVYLRTKISPSTDTLKVSKTPRQKKVTLSIDKDQEITD 120

Query: 121 YFNNIRLQWRFVCLRDERDVSNPEKQYFELLFPTKFRDTVVDFYLPYVLRRAEEIREEDK 180
           YF N RLQWRFVC +DER+ ++ EK++FEL+FP KFRD VVDFYLPYVLRRA+EI+EEDK
Sbjct: 121 YFENFRLQWRFVCSQDERNGNSREKRHFELIFPKKFRDRVVDFYLPYVLRRAKEIKEEDK 180

Query: 181 VVKIYSQQCEVYDYSNPGPKCWGSIILDHPATFETVAMDPDLKQWIIDDLDRFLRRKDFY 240
           VVKIYSQ+C+  D    G   WGSI LDHPATF+TVAM+P+LKQ+IIDDLDRF+RR+DFY
Sbjct: 181 VVKIYSQECQFDDDGGGGN--WGSINLDHPATFDTVAMEPELKQFIIDDLDRFVRRRDFY 240

Query: 241 KKVGKAWKRGYLLYGPPGTGKSSLIAAIANYLKFDIYDLDLASVYNNTILRRLLLATKNR 300
           KKVGKAWKRGYLLYGPPGTGKSSLIAA+ANYLKFDIYDLDL+++Y+N  LRR++LAT NR
Sbjct: 241 KKVGKAWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLSNIYSNADLRRVMLATTNR 300

Query: 301 SILVIEDIDCSANLQNRQSEEHFDASNSNSKLTLSGMLNFIDGLWSSCGDERIIIFTTNH 360
           SILVIEDIDCS ++QNRQ+EEHFD   S+SK TLSGMLNFIDGLWSSCGDERIIIFTTNH
Sbjct: 301 SILVIEDIDCSVDIQNRQNEEHFD--RSSSKFTLSGMLNFIDGLWSSCGDERIIIFTTNH 360

Query: 361 KERLDPALLRPGRMDVHINMSYCSPEGFKVLASNYLGGESVQHRLYGEIEELIRDMEVSP 420
           K+RLD ALLRPGRMDVHINMSYCSP GF+VLASNYLG E+ QHRLYGEIE+LIRDMEVSP
Sbjct: 361 KDRLDSALLRPGRMDVHINMSYCSPHGFRVLASNYLGEEATQHRLYGEIEDLIRDMEVSP 420

Query: 421 AEMAEELMKSEEADAVLEGLVVFLKRKREEQRKAKEEKESEKNEEREEREEMDKTETEEE 480
           AE+AEELMKS++ADAVL+GLV FLKRK+EEQRKAKEEK SEKNEE+EE+        E +
Sbjct: 421 AEIAEELMKSDDADAVLQGLVEFLKRKKEEQRKAKEEKASEKNEEKEEK--------EGD 480

Query: 481 EEEEEEKEKEEEEPKKLKWDMRIRRTGRGRR 512
           E EEEE+ + E EPK+L+   R RRTG G R
Sbjct: 481 EIEEEEEPEPEPEPKRLR-IRRPRRTGYGYR 496

BLAST of Spg012691 vs. ExPASy TrEMBL
Match: A0A1S3B2H6 (AAA-ATPase At2g18193-like OS=Cucumis melo OX=3656 GN=LOC103485078 PE=3 SV=1)

HSP 1 Score: 678.3 bits (1749), Expect = 2.5e-191
Identity = 373/511 (72.99%), Postives = 433/511 (84.74%), Query Frame = 0

Query: 4   FKEMVVPQSASAVFSAYASFATTIMLIRSITNELLPAQFLSFFSTIFFYFLGSSISSHTK 63
           FK+M VPQS SAVFSAYASFATT+MLIRS+TNELLPA+ +SF S+IF YF G SISS TK
Sbjct: 3   FKDMAVPQSVSAVFSAYASFATTMMLIRSVTNELLPAKLISFLSSIFVYFFG-SISSQTK 62

Query: 64  FVIDENSGLFHLNQVFQAAEIYLRTKISPSIQTLRVTKTPQQNKIMLTIDKDQEIIDYFN 123
           FVI+ENSG F +N+VFQAAE YLRTKISPSI TL+VTKTP+Q K+ L+IDKDQEIIDYF 
Sbjct: 63  FVIEENSG-FAMNEVFQAAEFYLRTKISPSIDTLKVTKTPRQKKVTLSIDKDQEIIDYFE 122

Query: 124 NIRLQWRFVCLRDERD-VSNPEKQYFELLFPTKFRDTVVDFYLPYVLRRAEEIREEDKVV 183
           NIRLQWRF+C  DER+     EK+ FELLFP KFRD VVDFYLPYVL+RA+EI+EE+KVV
Sbjct: 123 NIRLQWRFLCSVDERNGGGGREKRQFELLFPKKFRDRVVDFYLPYVLKRAKEIKEENKVV 182

Query: 184 KIYSQQCEVYDYSNPGPKCWGSIILDHPATFETVAMDPDLKQWIIDDLDRFLRRKDFYKK 243
           KI+SQ+C+ YD  + G   WGS+ L+HPATF+T+AMDP+LKQ II+DLDRF+RRKDFYKK
Sbjct: 183 KIFSQECQ-YDDDSGGN--WGSVNLEHPATFDTLAMDPELKQSIIEDLDRFVRRKDFYKK 242

Query: 244 VGKAWKRGYLLYGPPGTGKSSLIAAIANYLKFDIYDLDLASVYNNTILRRLLLATKNRSI 303
           VGKAWKRGYLLYGPPGTGKSSLIAA+ANYLKFDIYDLDL+++Y+N+ LRR+LLAT NRSI
Sbjct: 243 VGKAWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLSNMYSNSDLRRVLLATTNRSI 302

Query: 304 LVIEDIDCSANLQNRQSEEHFDASNSNSKLTLSGMLNFIDGLWSSCGDERIIIFTTNHKE 363
           LVIEDIDCS  +QNRQSEE+FD   S+SK TLSGMLNFIDGLWSSCGDERIIIFTTN+K 
Sbjct: 303 LVIEDIDCSVEIQNRQSEENFD--RSSSKFTLSGMLNFIDGLWSSCGDERIIIFTTNNKH 362

Query: 364 RLDPALLRPGRMDVHINMSYCSPEGFKVLASNYLGGESVQHRLYGEIEELIRDMEVSPAE 423
           RLDPALLR GRMD+HINMSYCS EG KVL SNYLGGE+ +H  YGEIEELI +MEV+PAE
Sbjct: 363 RLDPALLRAGRMDMHINMSYCSREGLKVLVSNYLGGEATKHSTYGEIEELIGEMEVAPAE 422

Query: 424 MAEELMKSEEADAVLEGLVVFLKRKREEQRKAKEEKESEKNEEREEREEMDKTETEEEEE 483
           +AEELMK EE +AVL GL+ FLKRKREEQRK KEEK+ EK EE ++          EEEE
Sbjct: 423 IAEELMKGEETEAVLGGLLGFLKRKREEQRKEKEEKKEEKGEEEDK---------IEEEE 482

Query: 484 EEEEKEKEEEEPKKLKWDM--RIRRTGRGRR 512
           E ++ E+EEE  KK+KW++  R+RR G G R
Sbjct: 483 EGDKIEEEEEATKKIKWELRNRVRRIGYGYR 497

BLAST of Spg012691 vs. ExPASy TrEMBL
Match: A0A0A0KGJ8 (AAA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G504460 PE=3 SV=1)

HSP 1 Score: 668.3 bits (1723), Expect = 2.5e-188
Identity = 370/511 (72.41%), Postives = 429/511 (83.95%), Query Frame = 0

Query: 4   FKEMVVPQSASAVFSAYASFATTIMLIRSITNELLPAQFLSFFSTIFFYFLGSSISSHTK 63
           FK+M VPQS SA+FSAYASFATT+MLIRS+TNELLPA+ +S FS+IF YF G SISS TK
Sbjct: 3   FKDMAVPQSVSALFSAYASFATTMMLIRSLTNELLPAKLIS-FSSIFVYFFG-SISSQTK 62

Query: 64  FVIDENSGLFHLNQVFQAAEIYLRTKISPSIQTLRVTKTPQQNKIMLTIDKDQEIIDYFN 123
            VI+ENSG F +N+VFQAAE YLRTKISPSI TL+VTKTP+Q K+ L+IDKDQEIIDYF 
Sbjct: 63  LVIEENSG-FAMNEVFQAAEFYLRTKISPSIDTLKVTKTPRQKKVTLSIDKDQEIIDYFE 122

Query: 124 NIRLQWRFVCLRDERD-VSNPEKQYFELLFPTKFRDTVVDFYLPYVLRRAEEIREEDKVV 183
           NIRLQWRF+C  DER+   + EK+ FEL FP KFRD +VDFYLPYVLRRA+EI+EE+KVV
Sbjct: 123 NIRLQWRFLCSVDERNGGGSREKRQFELSFPKKFRDRIVDFYLPYVLRRAKEIKEENKVV 182

Query: 184 KIYSQQCEVYDYSNPGPKCWGSIILDHPATFETVAMDPDLKQWIIDDLDRFLRRKDFYKK 243
           KI+SQ+C+ YD  + G   WGS+ L+HPATF+T+AMDP+LKQ II+DLDRF+RRKDFYKK
Sbjct: 183 KIFSQECQ-YDDDSGGN--WGSVNLEHPATFDTLAMDPELKQSIIEDLDRFVRRKDFYKK 242

Query: 244 VGKAWKRGYLLYGPPGTGKSSLIAAIANYLKFDIYDLDLASVYNNTILRRLLLATKNRSI 303
           VGKAWKRGYLL GPPGTGKSSLIAA+ANYLKFDIYDLDL ++Y+N+ LRR+LLAT NRSI
Sbjct: 243 VGKAWKRGYLLEGPPGTGKSSLIAAMANYLKFDIYDLDLTNMYSNSDLRRVLLATTNRSI 302

Query: 304 LVIEDIDCSANLQNRQSEEHFDASNSNSKLTLSGMLNFIDGLWSSCGDERIIIFTTNHKE 363
           LVIEDIDCS  +QNRQSEEHFD   S+SK TLSGMLNFIDGLWSSCGDERIIIFTTN+K 
Sbjct: 303 LVIEDIDCSVQIQNRQSEEHFD--QSSSKFTLSGMLNFIDGLWSSCGDERIIIFTTNNKH 362

Query: 364 RLDPALLRPGRMDVHINMSYCSPEGFKVLASNYLGGESVQHRLYGEIEELIRDMEVSPAE 423
           RLDPALLR GRMD+HINMSYCS EG +VL SNYLGGE+ +H  YGEIEELI +MEV+PAE
Sbjct: 363 RLDPALLRAGRMDMHINMSYCSREGLRVLVSNYLGGEATKHSTYGEIEELIGEMEVAPAE 422

Query: 424 MAEELMKSEEADAVLEGLVVFLKRKREEQRKAKEEKESEKNEEREEREEMDKTETEEEEE 483
           +AEELMK EE +AVL GLV FLKRKREE+RK KEEK+ EK EE E        + EEEEE
Sbjct: 423 IAEELMKGEETEAVLGGLVGFLKRKREEERKEKEEKKEEKGEEEE--------KVEEEEE 482

Query: 484 EEEEKEKEEEEPKKLKWDM--RIRRTGRGRR 512
           E ++  +EEE  KK  W++  R+RRTG G R
Sbjct: 483 EGDKIVEEEEATKKTNWELRNRVRRTGYGYR 497

BLAST of Spg012691 vs. ExPASy TrEMBL
Match: A0A6J1HFQ8 (AAA-ATPase At2g18193-like OS=Cucurbita moschata OX=3662 GN=LOC111463542 PE=3 SV=1)

HSP 1 Score: 641.0 bits (1652), Expect = 4.3e-180
Identity = 356/512 (69.53%), Postives = 417/512 (81.45%), Query Frame = 0

Query: 1   MFTFKEMVVPQSASAVFSAYASFATTIMLIRSITNELLPAQFLSFFSTIFFYFLGSSISS 60
           M  FKEM VPQSASAVFSAYASFATT+ML+RSIT+ELLP + +SFF +IFFYF G S+S 
Sbjct: 1   MLNFKEMAVPQSASAVFSAYASFATTMMLVRSITSELLPPKIISFFHSIFFYFFG-SVSF 60

Query: 61  HTKFVIDENSGLFHLNQVFQAAEIYLRTKISPSIQTLRVTKTPQQNKIMLTIDKDQEIID 120
             K +I+EN G F  NQ+FQAAE+YLRTKISPS +TL+  KT +Q KI L+IDK QEI+D
Sbjct: 61  QVKIIIEENCG-FSPNQIFQAAEVYLRTKISPSTETLKAHKTSRQKKITLSIDKGQEIVD 120

Query: 121 YFNNIRLQWRFVCLRDERD-VSNPEKQYFELLFPTKFRDTVVDFYLPYVLRRAEEIREED 180
           +F+NIRLQWRFVC  DE++   N EK++FEL+FP KFRD VV+ YLPYVL+RA+EI+EED
Sbjct: 121 HFDNIRLQWRFVCSHDEQNGGGNKEKRHFELVFPKKFRDRVVESYLPYVLKRAKEIKEED 180

Query: 181 KVVKIYSQQCEVYDYSNPGPKCWGSIILDHPATFETVAMDPDLKQWIIDDLDRFLRRKDF 240
           KVVKI+SQ+C+ YD  + G   WGSI LDHP+TFET+AMDPDLKQ IIDDLDRF+ R++F
Sbjct: 181 KVVKIFSQECQ-YD-EDGGSGNWGSINLDHPSTFETLAMDPDLKQSIIDDLDRFVNRREF 240

Query: 241 YKKVGKAWKRGYLLYGPPGTGKSSLIAAIANYLKFDIYDLDLASVYNNTILRRLLLATKN 300
           YKKVGKAWKRGYLLYGPPGTGKSSLIAA+ANYLKFDIYDLDL+ +Y+N  LRR+LL+T N
Sbjct: 241 YKKVGKAWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLSYIYSNGDLRRVLLSTTN 300

Query: 301 RSILVIEDIDCSANLQNRQSEEHFDASNSNSKLTLSGMLNFIDGLWSSCGDERIIIFTTN 360
           RSILVIEDIDCS  +QNR++EEH+D   SN K TLSGMLNFIDGLWSSCGDERIIIFTTN
Sbjct: 301 RSILVIEDIDCSVEIQNRENEEHYD--QSNGKFTLSGMLNFIDGLWSSCGDERIIIFTTN 360

Query: 361 HKERLDPALLRPGRMDVHINMSYCSPEGFKVLASNYLGGESVQHRLYGEIEELIRDMEVS 420
           HKERLDPALLRPGRMDVHINMSYC+P GF+VL +NYLG E+ +H L  EIEELI +MEVS
Sbjct: 361 HKERLDPALLRPGRMDVHINMSYCTPHGFRVLVTNYLGEEADKHDLCREIEELIGEMEVS 420

Query: 421 PAEMAEELMKSEEADAVLEGLVVFLKRKREEQRKAKEE-KESEKNEEREEREEMDKTETE 480
           PAE+AE LMK+++AD VL+ LV FLK KREEQRK +EE   SEKNEE            E
Sbjct: 421 PAEIAEALMKNDDADVVLQSLVEFLKCKREEQRKKEEETAASEKNEEE-----------E 480

Query: 481 EEEEEEEEKEKEEEEPKKLKWDMRIRRTGRGR 511
           EEEEEEE K K  +   +L+   R R  GRGR
Sbjct: 481 EEEEEEEPKRKRHKRGVRLRIG-RGRGRGRGR 494

BLAST of Spg012691 vs. ExPASy TrEMBL
Match: A0A5D3CMN5 (AAA-ATPase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G001760 PE=3 SV=1)

HSP 1 Score: 640.2 bits (1650), Expect = 7.4e-180
Identity = 330/438 (75.34%), Postives = 380/438 (86.76%), Query Frame = 0

Query: 4   FKEMVVPQSASAVFSAYASFATTIMLIRSITNELLPAQFLSFFSTIFFYFLGSSISSHTK 63
           FK+M VPQS SAVFSAYASFATT+MLIRS+TNELLPA+ +SF S+IF YF G SISS TK
Sbjct: 3   FKDMAVPQSVSAVFSAYASFATTMMLIRSVTNELLPAKLISFLSSIFVYFFG-SISSQTK 62

Query: 64  FVIDENSGLFHLNQVFQAAEIYLRTKISPSIQTLRVTKTPQQNKIMLTIDKDQEIIDYFN 123
           FVI+ENSG F +N+VFQAAE YLRTKISPSI TL+VTKTP+Q K+ L+IDKDQEIIDYF 
Sbjct: 63  FVIEENSG-FAMNEVFQAAEFYLRTKISPSIDTLKVTKTPRQKKVTLSIDKDQEIIDYFE 122

Query: 124 NIRLQWRFVCLRDERD-VSNPEKQYFELLFPTKFRDTVVDFYLPYVLRRAEEIREEDKVV 183
           NIRLQWRF+C  DER+     EK+ FELLFP KFRD VVDFYLPYVL+RA+EI+EE+KVV
Sbjct: 123 NIRLQWRFLCSVDERNGGGGREKRQFELLFPKKFRDRVVDFYLPYVLKRAKEIKEENKVV 182

Query: 184 KIYSQQCEVYDYSNPGPKCWGSIILDHPATFETVAMDPDLKQWIIDDLDRFLRRKDFYKK 243
           KI+SQ+C+ YD  + G   WGS+ L+HPATF+T+AMDP+LKQ II+DLDRF+RRKDFYKK
Sbjct: 183 KIFSQECQ-YDDDSGGN--WGSVNLEHPATFDTLAMDPELKQSIIEDLDRFVRRKDFYKK 242

Query: 244 VGKAWKRGYLLYGPPGTGKSSLIAAIANYLKFDIYDLDLASVYNNTILRRLLLATKNRSI 303
           VGKAWKRGYLLYGPPGTGKSSLIAA+ANYLKFDIYDLDL+++Y+N+ LRR+LLAT NRSI
Sbjct: 243 VGKAWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLSNMYSNSDLRRVLLATTNRSI 302

Query: 304 LVIEDIDCSANLQNRQSEEHFDASNS-----NSKLTLSGMLNFIDGLWSSCGDERIIIFT 363
           LVIEDIDCS  +QNRQSEE+FD S+S       + TLSGMLNFIDGLWSSCGDERIIIFT
Sbjct: 303 LVIEDIDCSVEIQNRQSEENFDRSSSKYFVYKLQFTLSGMLNFIDGLWSSCGDERIIIFT 362

Query: 364 TNHKERLDPALLRPGRMDVHINMSYCSPEGFKVLASNYLGGESVQHRLYGEIEELIRDME 423
           TN+K RLDPALLR GRMD+HINMSYCS EG KVL SNYLGGE+ +H  YGEIEELI +ME
Sbjct: 363 TNNKHRLDPALLRAGRMDMHINMSYCSREGLKVLVSNYLGGEATKHSTYGEIEELIGEME 422

Query: 424 VSPAEMAEELMKSEEADA 436
           V+PAE+AEELMK EE +A
Sbjct: 423 VAPAEIAEELMKGEETEA 435

BLAST of Spg012691 vs. TAIR 10
Match: AT2G18193.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 433.3 bits (1113), Expect = 2.6e-121
Identity = 247/487 (50.72%), Postives = 351/487 (72.07%), Query Frame = 0

Query: 12  SASAVFSAYASFATTIMLIRSITNELLPAQFLSFFSTIFFYFLGSSISSHTKFVIDENSG 71
           S S++FSAYAS    +ML RS+ ++ +P +  S+FS++   F  +  S +   +IDEN G
Sbjct: 10  SPSSLFSAYASLTGFLMLFRSMLHDFVPEKLRSYFSSLLDRFF-TPKSKYLTVIIDENFG 69

Query: 72  LFHLNQVFQAAEIYLRTKISPSIQTLRVTKTPQQNKIMLTIDKDQEIIDYFNNIRLQWRF 131
           L + NQVF AAE+YLR+KI P  + LRV K P+Q    ++I++ +EI+D F    ++W +
Sbjct: 70  L-NRNQVFDAAEMYLRSKIGPETERLRVGKIPKQKHFTISIERGEEILDTFEESEVKWSY 129

Query: 132 VCLRDERDVSNPEKQYFELLFPTKFRDTVVDFYLPYVLRRAEEIREEDKVVKIYSQQCEV 191
           V  + E +  +  K+Y+EL F  K RD V++ YL +V+  +EEI+   +VVK+YS+  +V
Sbjct: 130 V--QSENEKGDKVKRYYELTFEKKLRDKVLNSYLTHVVAESEEIKRNLRVVKLYSR--DV 189

Query: 192 Y---DYSNPGPKCWGSIILDHPATFETVAMDPDLKQWIIDDLDRFLRRKDFYKKVGKAWK 251
           Y   D        WG I L+HP+TF+T+AMDP+ K+ IIDDL+RFL+RK+FYK+VGKAWK
Sbjct: 190 YASDDDDGMAGGNWGCINLEHPSTFDTLAMDPNAKKKIIDDLERFLKRKEFYKRVGKAWK 249

Query: 252 RGYLLYGPPGTGKSSLIAAIANYLKFDIYDLDLASVYNNTILRRLLLATKNRSILVIEDI 311
           RGYLLYGPPGTGKSSLIAA+ANYLKFD++DL+L+S+Y+N  L+R+LL+T NRSILVIEDI
Sbjct: 250 RGYLLYGPPGTGKSSLIAAMANYLKFDVFDLELSSIYDNGELKRVLLSTTNRSILVIEDI 309

Query: 312 DCSANLQNRQSEEHFDASNSNSKLTLSGMLNFIDGLWSSCGDERIIIFTTNHKERLDPAL 371
           DC+A +++R++E   D      K+TLSG+LNFIDGLWSS GDERII+FTTNHKERLDPAL
Sbjct: 310 DCNAEVRDREAENQED-EQIKGKVTLSGILNFIDGLWSSFGDERIIVFTTNHKERLDPAL 369

Query: 372 LRPGRMDVHINMSYCSPEGFKVLASNYLGGESVQHRLYGEIEELIRDMEVSPAEMAEELM 431
           LRPGRMDVHINMSYC+  GF+ L SNYLG + + H L  EIE L+   EV+PAE+AEELM
Sbjct: 370 LRPGRMDVHINMSYCTGLGFRTLVSNYLGLDGLNHPLCEEIEALVDSTEVTPAELAEELM 429

Query: 432 KSEEADAVLEGLVVFLKRKREEQRKAKEEKESEKNEEREEREEMDKTETEEEEEEEEEKE 491
           + ++ D VL G++ F+++++ E+ K K+E    K  + +E++       +++++  ++K 
Sbjct: 430 QDDDTDVVLRGVISFVEKRKVERSKTKKEVSICKATDDDEKQNGSLGCVKKKKKGGKQKG 489

Query: 492 KEEEEPK 496
           K + + K
Sbjct: 490 KGKGKGK 489

BLAST of Spg012691 vs. TAIR 10
Match: AT3G50940.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 432.6 bits (1111), Expect = 4.5e-121
Identity = 230/448 (51.34%), Postives = 320/448 (71.43%), Query Frame = 0

Query: 12  SASAVFSAYASFATTIMLIRSITNELLPAQFLSFFSTIFFYFLGSSISSHTKFVIDENSG 71
           +A    +A AS A   +L RS+  + +P +   + S  F  F  S  S     VI+E  G
Sbjct: 11  TAKTALTAVASVAAAAILARSVVQDYMPNEVHEYISHGFRRFF-SYFSYQMTAVIEEFGG 70

Query: 72  LFHLNQVFQAAEIYLRTKISPSIQTLRVTKTPQQNKIMLTIDKDQEIIDYFNNIRLQWRF 131
             H NQVF+AAE YL TKIS S + ++V K  +Q+   +T+++D+E++D F+ ++L W  
Sbjct: 71  FEH-NQVFEAAEAYLSTKISNSTRRIKVNKLEKQSNYSVTVERDEEVVDIFDGVKLSWIL 130

Query: 132 VCLR-DERDVSNP---------EKQYFELLFPTKFRDTVVDFYLPYVLRRAEEIREEDKV 191
           VC   D++D  NP         E + +EL F  KF++ V++ YLP+V+ +A  I+++ K 
Sbjct: 131 VCRHVDKKDFRNPRDLNSTLKSEVRSYELSFRKKFKNMVLESYLPFVVEQAASIKQKFKT 190

Query: 192 VKIYSQQCEVYDYSNPGPKCWGSIILDHPATFETVAMDPDLKQWIIDDLDRFLRRKDFYK 251
           +KI++    V  YS      W S+ LDHP+TF T+A+DP++K+ +++DLDRF++RK FY 
Sbjct: 191 LKIFT----VDSYSVE----WTSVTLDHPSTFRTLALDPEVKKNLVEDLDRFVQRKGFYG 250

Query: 252 KVGKAWKRGYLLYGPPGTGKSSLIAAIANYLKFDIYDLDLASVYNNTILRRLLLATKNRS 311
           +VGKAWKRGYLLYGPPGTGKSSLIAAIAN+L FDIYDLDL S+ NN  LRRLL++T NRS
Sbjct: 251 RVGKAWKRGYLLYGPPGTGKSSLIAAIANHLNFDIYDLDLTSLNNNAELRRLLMSTANRS 310

Query: 312 ILVIEDIDCSANLQNRQSEEHFDASNSNSKLTLSGMLNFIDGLWSSCGDERIIIFTTNHK 371
           ILV+EDIDCS  L++R +++  +    +  +TLSG+LNF+DGLWSSCG+ERII+FTTN++
Sbjct: 311 ILVVEDIDCSIELKDRSTDQE-NNDPLHKTVTLSGLLNFVDGLWSSCGNERIIVFTTNYR 370

Query: 372 ERLDPALLRPGRMDVHINMSYCSPEGFKVLASNYLGGESVQHRLYGEIEELIRDMEVSPA 431
           E+LDPALLRPGRMD+HI+MSYC+P  FKVLASNYL  E   H L+ +IEE IR++EV+PA
Sbjct: 371 EKLDPALLRPGRMDMHIHMSYCTPAAFKVLASNYL--EIQDHILFEQIEEFIREIEVTPA 430

Query: 432 EMAEELMKSEEADAVLEGLVVFLKRKRE 450
           E+AE+LM+S+  D VL+GLV FLK K++
Sbjct: 431 EVAEQLMRSDSVDKVLQGLVEFLKAKKQ 445

BLAST of Spg012691 vs. TAIR 10
Match: AT3G50930.1 (cytochrome BC1 synthesis )

HSP 1 Score: 418.7 bits (1075), Expect = 6.8e-117
Identity = 250/503 (49.70%), Postives = 350/503 (69.58%), Query Frame = 0

Query: 12  SASAVFSAYASFATTIMLIRSITNELLPAQ---FLSF-FSTIFFYFLGSSISSHTKFVID 71
           +A  V +  AS A T ML RS+  + LP +   ++S+ F +IF YF     SS    +I+
Sbjct: 57  TAKTVLTTAASVAATAMLARSLVQDYLPDEVHHYISYGFRSIFGYF-----SSQMTIIIE 116

Query: 72  ENSGLFHLNQVFQAAEIYLRTKISPSIQTLRVTKTPQQNKIMLTIDKDQEIIDYFNNIRL 131
           E  G  H N+VF+AAE YL TKISPS + ++V+K  ++N   +T+++D+E++D +N ++ 
Sbjct: 117 EFEGFAH-NEVFEAAEAYLATKISPSNKRIKVSKHEKENNYNVTVERDEEVVDTYNGVKF 176

Query: 132 QWRFVCL----------RDERDVSNPEKQYFELLFPTKFRDTVVDFYLPYVLRRAEEIRE 191
           QW   C           RD       E + FEL F  KF+D  ++ YLP++++RA  +++
Sbjct: 177 QWILHCRHVESKHFHNPRDLNSTLRSEVRSFELNFHKKFKDVALESYLPFMVKRATLMKQ 236

Query: 192 EDKVVKIYSQQCE-VY-DYSNPGPKCWGSIILDHPATFETVAMDPDLKQWIIDDLDRFLR 251
           E K +KI++   E +Y +YS+     W S+ LDHP+TF+T+AMD D+K  +++DLD+F++
Sbjct: 237 EKKTLKIFTLSPENMYGNYSD----AWTSVTLDHPSTFKTLAMDSDVKTSVMEDLDKFVK 296

Query: 252 RKDFYKKVGKAWKRGYLLYGPPGTGKSSLIAAIANYLKFDIYDLDLASVYNNTILRRLLL 311
           R+DFYK+VGKAWKRGYLLYGPPGTGKSSLIAA+AN+L FDIYDL+L +V NN+ LRRLL+
Sbjct: 297 RRDFYKRVGKAWKRGYLLYGPPGTGKSSLIAAMANHLNFDIYDLELTAVNNNSELRRLLI 356

Query: 312 ATKNRSILVIEDIDCSANLQNRQSEEHFDASNS-----NSKLTLSGMLNFIDGLWSSCGD 371
           AT NRSIL++EDIDCS  L++R S+E    S+        K+TLSG+LNFIDGLWSSCGD
Sbjct: 357 ATANRSILIVEDIDCSLELKDRTSDEPPRESDDIEDPRYKKVTLSGLLNFIDGLWSSCGD 416

Query: 372 ERIIIFTTNHKERLDPALLRPGRMDVHINMSYCSPEGFKVLASNYLGGESVQHRLYGEIE 431
           ERIIIFTTN+KE+LD ALLRPGRMD+HI+MSYC+P  FK LA NYL  E  +HRL+ +IE
Sbjct: 417 ERIIIFTTNYKEKLDAALLRPGRMDMHIHMSYCTPSTFKALALNYL--EIKEHRLFSKIE 476

Query: 432 ELIRDMEVSPAEMAEELMKSEEADAVLEGLVVFLKRKR--EEQRKAKEEKESEKNEE--R 490
           E I   EV+PAE+AE+LM+++  D VLEGL+ FLK K+   EQ KAK EK+  +N++  +
Sbjct: 477 EGIEATEVTPAEVAEQLMRNDSVDKVLEGLIEFLKVKKIENEQDKAKTEKQELENKKKTK 536

BLAST of Spg012691 vs. TAIR 10
Match: AT2G18190.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 417.5 bits (1072), Expect = 1.5e-116
Identity = 241/482 (50.00%), Postives = 334/482 (69.29%), Query Frame = 0

Query: 12  SASAVFSAYASFATTIMLIRSITNELLPAQFLSFFSTIFFYFLGSSISSHTKFVIDENSG 71
           S S++F+AYAS    +ML RS+ N+ +P +  S+ + +   F  +  S +   VIDE  G
Sbjct: 11  SPSSLFTAYASLTGFLMLFRSLFNDEVPERLRSYITDLLNRFF-TPKSKNLTMVIDEIIG 70

Query: 72  LFHLNQVFQAAEIYLRTKISPSIQTLRVTKTPQQNKIMLTIDKDQEIIDYFNNIRLQWRF 131
            F  NQVF AAE+YLR KI P    LRV K P+Q    + I+K +EI+D F N  L+W +
Sbjct: 71  -FKRNQVFDAAEVYLRNKIGPETARLRVGKLPKQKHFTIYIEKGEEILDTFENSELRWTY 130

Query: 132 VCLRDERDVSNPEKQYFELLFPTKFRDTVVDFYLPYVLRRAEEIREEDKVVKIYSQQCEV 191
           V    E + S  EK+Y+EL F  K RD V++ YL +V+  +EE + + + VK+YS+    
Sbjct: 131 V--ESENEASQKEKRYYELTFEKKLRDKVMNSYLSHVVAESEETKRDLRAVKLYSRDVRA 190

Query: 192 -YDYSNPGPKCWGSIILDHPATFETVAMDPDLKQWIIDDLDRFLRRKDFYKKVGKAWKRG 251
             D        WG I L+HP+TFET+AMDP  K+ IIDD++RFL+R++FYK+VGKAWKRG
Sbjct: 191 SKDDDGMAGAGWGCINLEHPSTFETLAMDPGAKKKIIDDMERFLKRREFYKRVGKAWKRG 250

Query: 252 YLLYGPPGTGKSSLIAAIANYLKFDIYDLDLASVYNNTILRRLLLATKNRSILVIEDIDC 311
           YLLYGPPGTGKSSLIAA+ANYLKFD++DL+L+S+Y N  L+ +LL+T NRSILVIEDIDC
Sbjct: 251 YLLYGPPGTGKSSLIAAMANYLKFDVFDLELSSIYENAQLKSILLSTTNRSILVIEDIDC 310

Query: 312 -SANLQNRQSEEHFDASNS-NSKLTLSGMLNFIDGLWSSCGDERIIIFTTNHKERLDPAL 371
            SA + +R+++E+ +       ++TLSG+LNF+DGLWSS GDERII+FTTNHKERLDPAL
Sbjct: 311 SSAEVVDREADEYQEYEEGYYGRVTLSGLLNFVDGLWSSFGDERIIVFTTNHKERLDPAL 370

Query: 372 LRPGRMDVHINMSYCSPEGFKVLASNYLGGESVQHRLYGEIEELIRDMEVSPAEMAEELM 431
           LRPGRMD+HINMSYC+  GF+ L SNYLG   + H L  EIE LI   EV+PAE+AEELM
Sbjct: 371 LRPGRMDMHINMSYCTGLGFRTLVSNYLGLGGLNHPLCEEIEALIDSTEVTPAELAEELM 430

Query: 432 KSEEADAVLEGLVVFLKRKREEQRKAKE-EKESEKNEEREEREEMDKTETEEEEEEEEEK 490
           + ++ D VL G+V F++ ++ E  K KE E  + +  + +++  +  T   ++ +++++ 
Sbjct: 431 QEDDTDVVLRGVVSFVENRKVEISKTKELEGSTCRKLDGDDKHNVSSTNDLKKTKKKKKG 488

BLAST of Spg012691 vs. TAIR 10
Match: AT5G17760.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 412.1 bits (1058), Expect = 6.3e-115
Identity = 252/515 (48.93%), Postives = 347/515 (67.38%), Query Frame = 0

Query: 1   MFTFKEMVVPQSASAVFSAYASFATTIMLIRSITNELLPAQFLSF-FSTIFFYFLGSSIS 60
           MF  K++  P S   VF+AYAS A  +M+IRS+ +EL+PA    F + T+   F  SS S
Sbjct: 1   MFFSKDLPSPTS---VFTAYASMAGYMMMIRSMAHELIPAPLQDFIYRTLRSLFFRSSSS 60

Query: 61  SHTKFVIDENSGLFHLNQVFQAAEIYLRTKISPSIQTLRVTKTPQQNKIMLTIDKDQEII 120
           + T  + D+N G+   N++++AA+ YL TKISP    LR++K  +   + L +   + + 
Sbjct: 61  TLTLTIDDDNMGM--NNEIYRAAQTYLSTKISPDAVRLRISKGHKDKHVNLYLSDGEIVN 120

Query: 121 DYFNNIRLQWRFVCLRDERD-------------------VSNPEKQYFELLFPTKFRDTV 180
           D + +++L WRFV    ++                      + + +YFEL F  K +D +
Sbjct: 121 DVYEDVQLVWRFVTDGGDKKGGGGGVGGRGGGGGRRGGMDDDGKSEYFELSFDKKHKDLI 180

Query: 181 VDFYLPYVLRRAEEIREEDKVVKIYSQQCEVYDYSNPGPKCWGSIILDHPATFETVAMDP 240
           ++ Y+PY+  +A+EIR+E +++ ++S               W S+IL+HP+TFET+AM+ 
Sbjct: 181 LNSYVPYIESKAKEIRDERRILMLHSL----------NSLRWESVILEHPSTFETMAMED 240

Query: 241 DLKQWIIDDLDRFLRRKDFYKKVGKAWKRGYLLYGPPGTGKSSLIAAIANYLKFDIYDLD 300
           DLK+ +I+DLDRF+RRK+FYK+VGKAWKRGYLLYGPPGTGKSSL+AA+ANYLKFD+YDL 
Sbjct: 241 DLKRDVIEDLDRFIRRKEFYKRVGKAWKRGYLLYGPPGTGKSSLVAAMANYLKFDVYDLQ 300

Query: 301 LASVYNNTILRRLLLATKNRSILVIEDIDCSANLQNR--QSEEHFDASNSNSKLTLSGML 360
           LASV  ++ LRRLLLAT+NRSILVIEDIDC+ +L NR  Q  E  +   S   LTLSG+L
Sbjct: 301 LASVMRDSDLRRLLLATRNRSILVIEDIDCAVDLPNRIEQPVEGKNRGESQGPLTLSGLL 360

Query: 361 NFIDGLWSSCGDERIIIFTTNHKERLDPALLRPGRMDVHINMSYCSPEGFKVLASNYLG- 420
           NFIDGLWSSCGDERIIIFTTNHK+RLDPALLRPGRMD+HI M +CS +GFK LASNYLG 
Sbjct: 361 NFIDGLWSSCGDERIIIFTTNHKDRLDPALLRPGRMDMHIYMGHCSFQGFKTLASNYLGL 420

Query: 421 -GESVQHRLYGEIEELIRDMEVSPAEMAEELMKSEEADAVLEGLVVFLKRKREEQRKAKE 480
              ++ HRL+ EIE LI    ++PA++AEELMKSE+AD  LEGLV  L++ R + +++  
Sbjct: 421 SDAAMPHRLFPEIERLIDGEVMTPAQVAEELMKSEDADVALEGLVNVLEKMRLKSKESNP 480

Query: 481 EKESEKNEEREEREEMD-KTETEEEEEEEEEKEKE 491
               +K E R E EEM  K++TE    +  ++ K+
Sbjct: 481 VMMKQK-ESRLEMEEMRLKSDTEGSPRKNSKRFKK 499

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022132942.13.7e-20275.15AAA-ATPase At2g18193-like, partial [Momordica charantia][more]
XP_038877872.18.4e-19473.97AAA-ATPase At2g18193-like [Benincasa hispida][more]
XP_008440759.15.1e-19172.99PREDICTED: AAA-ATPase At2g18193-like [Cucumis melo][more]
XP_004149988.34.8e-18972.60AAA-ATPase At2g18193 [Cucumis sativus] >KGN49285.2 hypothetical protein Csa_0032... [more]
XP_038877870.13.5e-18472.29AAA-ATPase At2g18193-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q8GW963.7e-12050.72AAA-ATPase At2g18193 OS=Arabidopsis thaliana OX=3702 GN=At2g18193 PE=2 SV=1[more]
Q147F96.4e-12051.34AAA-ATPase At3g50940 OS=Arabidopsis thaliana OX=3702 GN=At3g50940 PE=2 SV=1[more]
Q8VZG29.5e-11649.70Protein HYPER-SENSITIVITY-RELATED 4 OS=Arabidopsis thaliana OX=3702 GN=HSR4 PE=2... [more]
F4IQG22.1e-11550.00AAA-ATPase At2g18190 OS=Arabidopsis thaliana OX=3702 GN=At2g18190 PE=2 SV=1[more]
Q9FN758.9e-11448.93AAA-ATPase At5g17760 OS=Arabidopsis thaliana OX=3702 GN=At5g17760 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1BV861.8e-20275.15AAA-ATPase At2g18193-like OS=Momordica charantia OX=3673 GN=LOC111005662 PE=3 SV... [more]
A0A1S3B2H62.5e-19172.99AAA-ATPase At2g18193-like OS=Cucumis melo OX=3656 GN=LOC103485078 PE=3 SV=1[more]
A0A0A0KGJ82.5e-18872.41AAA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G504460 PE=3 SV... [more]
A0A6J1HFQ84.3e-18069.53AAA-ATPase At2g18193-like OS=Cucurbita moschata OX=3662 GN=LOC111463542 PE=3 SV=... [more]
A0A5D3CMN57.4e-18075.34AAA-ATPase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G001760 PE... [more]
Match NameE-valueIdentityDescription
AT2G18193.12.6e-12150.72P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
AT3G50940.14.5e-12151.34P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
AT3G50930.16.8e-11749.70cytochrome BC1 synthesis [more]
AT2G18190.11.5e-11650.00P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
AT5G17760.16.3e-11548.93P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 441..493
NoneNo IPR availableGENE3D6.10.280.40coord: 379..451
e-value: 2.6E-19
score: 70.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 449..470
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 471..489
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 449..500
NoneNo IPR availablePANTHERPTHR23070BCS1 AAA-TYPE ATPASEcoord: 10..451
NoneNo IPR availablePANTHERPTHR23070:SF140BNAA06G25360D PROTEINcoord: 10..451
NoneNo IPR availableCDDcd00009AAAcoord: 248..382
e-value: 2.53275E-13
score: 65.6303
IPR003593AAA+ ATPase domainSMARTSM00382AAA_5coord: 247..384
e-value: 4.8E-8
score: 42.7
IPR025753AAA-type ATPase, N-terminal domainPFAMPF14363AAA_assoccoord: 38..133
e-value: 3.7E-17
score: 62.4
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 205..378
e-value: 7.6E-45
score: 154.9
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 200..429
IPR003959ATPase, AAA-type, corePFAMPF00004AAAcoord: 252..381
e-value: 4.8E-19
score: 69.1
IPR003960ATPase, AAA-type, conserved sitePROSITEPS00674AAAcoord: 352..370

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg012691.1Spg012691.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006952 defense response
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0043531 ADP binding
molecular_function GO:0005524 ATP binding
molecular_function GO:0016887 ATP hydrolysis activity