Spg012550 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg012550
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Description2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
Locationscaffold1: 5014863 .. 5018756 (+)
RNA-Seq ExpressionSpg012550
SyntenySpg012550
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTTTGCGCGTCGGCAGAAGGTTCTTCTCCCGACGCAGTTAACGGCGTCGAGAGAAATCGCGTCGGCAAAAACAATTTTTCTTGTAGTGCGAACGGGGGCGTAAGGACTCGACCCACGCAAGTGGGCCGAGTGGTCGCCCTTGGCCTTGGGTTGAGGCAGACCATATGGGTTGGGCCAAGGCCCGATCCCTCCGACCTTGGCCCGACCCTCTGGCCTGTTTCTCCTCCGGAGCCTGTTTCCTGGTTGTCTTCTTGGGCTGGTGCTACGTTGTCCTGCTTAGTCCTTGGATACCGGGGTGGTCTGGAATCATCCTCAACAGGATCCATTTTGAATACCTTTTAATTTAAGTGTTTTTCAAGAATGCATATTTTAAAAAAATATATTTTGTATACAAATTGTTTAGAATTAAAACACTCATATGTTTGGTTACATCTCCTTAGAGATGTTTTTATACACAAGTTATCATATGTTTAAAAACATTTTTTTAAAAAAATTGTTTTCAATTGTTTCAGTCAAAATGATTTATTTCTCGATCACATTTTCTAATAAGTCATTTTTTGTCGTTCCCAAACACTAGAAGTTGTTTTAAAATGATTTCCTTTCGAAATTAAACACTTGAAAATTTAAACAAAAAATACTATTAAACTAGAAGGTATTCCAAACGGAGACTAAACCACCATTTTAATAAAAGTAAAAGAAAAGGAGAGATTTAAACCAATAATTTTTTTCCCTAAAAAGAATTCTATATAATTGAAAAATCTCGAAAAGAAATAAAGCCGCCGGAGACAGAGGGAGAAAAATTAAATAAATAATTAATAAATAAAACTTCTCAGATCGCTGAAAGCTAAGCCAAAGCCAAGCCAAAACCAAAACCAAAATCAAAATGAATCTTTAATTACGGTGTCAAAGTTCACCAAGTTTTCCAGCACAGAGGCCTTGGACTTTCGAGACGAAGACCAAAATCAACCGATACTCTCCTCCAAAGGGACTCTGCAATTCCAGATCTTGCTTCCAAGGGTTCTGTTTCCATGGCGAAGCACAGGCAATCTCGTTTTCCTTCTCGGAAGTCCTCTTCTTCTTCTACTCTCGTCTTTACCCTGCTTATTATGTTCACCTTCGTCATTCTCATTCTTCTTGCCCTTGGAATCCTCTCGATCCCCGGCAATTCCGGCGGTTCGCCCAAGGTTCATGATCTGAGCTCGATTGTGCGCAAAACTTCGGACGAGTATGTGATCTTCTGGGTTTGCTTTTGATTTTTTGGTTTGTATAGGGTTGGTTTTTTATTGATTGTGTGGTTTTGTTTGATTTTGTTCTAGTGTTGACGAGGAGAAAGGGGAGCAGTGGGTCGAAGTGATCTCATGGGAACCTAGAGCATTCGTTTATCATAACTTTCTGGTATCGTTTCTTCCGATCTATGATGAGAGATTTGTGTTTTATGTTCATTCATCTCTCTGTATGCAATTTGTGTATGGAATTCTTTAAACTTGGTTGGGTTATGCTGATTCATGTTGAATGCGTCTTTGGGGCTGCACATGCTGATGAAATCCAAGGGCATTTTTGAAATGAAGTCTTTGGGGCTGCACATCCTTCAAGTTGATTATGTTTTTATGTTCTTTTAAGAAGTCATTCTTCTGTCTCGTTATGCTCACAGGGGTTAAGGATAATTCCCATCTGTATTTGTCTTTAGATTGTATTGGAGTTTGAGTTGACCGAGGCCCATTAATTAATAGGAATTACTGAATTGGTTTACTTTTTAATTGCTCAGACAAAGGAGGAATGTGAGTACTTAATCAGCCTTGCCAAGCCTCACATGCAAAAGTCTACCGTTGTTGACAGTGAAACTGGAAAGAGCAAAGATAGCAGGTCTGTGAAATTCTCCTCCCCAAAATTCTCCATTGTTAGTAATGCTGGATCTCAAATGTAAAAACCTTCCATGACCTCTCTTTTACAGAGTTCGCACTAGTTCTGGAACATTTTTGCCGAGAGGACGTGATAAAACTATTAGAACCATTGAGAAAAGGATTGCTGATTTCACCTTCATACCCGTAGGTACATTGTTTGCGCCTATTTCTCACTACATCAAGCATTATTTTTTATCATGCATGGTTAAAATTAAACTTTGTAATCTTTGAGTTATTTCCAAGTGCAACTATCACTGTGGTATATTTTTTCCCATATAATCTAGCATTCCCAATCATAACCTTTCTTTTGTTGGAAAAAGTCTTTTACAATCATGCCCAAAACCATTCAAATAGACACAATTACTGTGCTAAGTTTCACATGGATGAGGAAGATTTTTAATCATGGTTGAAAAGTTGAATAATGAATATCCACTCGGATGTAGGAACACCAAAATGATCCAGATATTTATTCCTATGAGCTTACCCACCCATTCTCCCTTTTAAGAATGAAAGAGAACCTGATTAGATGTGCCAATATTGGTTGATCATATGTAGATATTGTCTTGTTATCTTTTGAAATTTTCATAAAATCCATAAATATTAGACCCTTCTTTTTCATATTTTGTTACTGAAGCTCGCACATAATATATTCAGAGCATGGAGAAGGACTTCAGGTTCTTCATTACGAAGTGGGACAGAAATATGAACCTCATTTTGATTACTTCCTTGATGACTACAATACAAAGAATGGAGGTCAACGTATAGCAACAGTGCTTATGTATCTGTAAGTAGTAATCCTTATTTATGTCCACTATGCTTTGGACTCCAACCAAGTGCTATTATATCTTGCACGGTTCTCCCATTATTTTATATGACTTCTATCATAATTCAGCTCAGAAGTTGAAGAAGGAGGCGAGACAGTGTTCCCTGCTGCCAAAGGAAACTTTAGTTCTGTACCTTGGTGGAATGAGCTTTCAGATTGTGGGAAGAAAGGACTTTCTGTTAAACCGAAGAGGGGTGATGCGCTGCTTTTCTGGAGCATGAAGCCTGATGCTTCTCTTGATCCATCAAGTTTGCATGGTTAGATTACATTCTCTTAACTTATAATCTACCATTCATTTTACGGAGCATGATAAGTTTCAGCGATGCACATTTATTTTTGCCAGTGGGATTTCTTTCGCATCTCTTATGTGGAAAATAATGGATTTTCTTGATGAAATGACTATATTTCTTCTGCTGTTCATTTATAGGTGGTTGCCCTGTTATCAAGGGGAATAAATGGTCTGCTACTAAATGGATGCGAGTAGAAGAATACAAAGCTTGAGTTGATGGGACTAGGGTAATTTCCATGCTCTAAACCACTGAATCATTTTCGCATTAAGTATGCTGTAATTTTGAACTCCCCCCACCCACCCAAAAGAAAAGAACAAAGGAATTTTCTTTGTTGTATTTTGTTGTTATTGCTCAGAGAATTATTCCTAAAAATTTGATGCTTCATTGTTTGGCAGGTTTGGTTGACTTCTCTTGTTACTGCTTAAAGTCAGATGCATTTGCTGTAGGGAATCATCATTTCTATAATTTAACCTTGTGCGCGACTACTCGATCCCTAAATCTATGAATTTTGATGTGCTTAATATTTTAATTACGAGCTTTACATTGTCATTGATTTCTTGTTTATGGCCACGAGAGAATAAGAGTTCATATTTTTGCCAGAAATGCCACCTCCAAACCTCAGTTATAGATTCATATGGTATGTAAAAAGAACAATTTTTACGCTTGATGTACAACTCTCTTGCTGCAACATTGTTATATTTTTCTAGTTGATATGACCTTTTAATGCCTAATCATTCTTCCAACTAGCTAGCCATTTGGAACGAAAATTGGGATAAGGGATCTTCCAAATTTGTTTTTGTTTTTATTTTTTGTGTTCAGTTTTTATTTCTAATATATTCTTCTAAGCTAAATTTTAGATTTTCTTTGTGTCTCCATT

mRNA sequence

ATGGTTTGCGCGTCGGCAGAAGGTTCTTCTCCCGACGCAGTTAACGGCGTCGAGAGAAATCGCGTCGGCAAAAACAATTTTTCTTGTAGTGCGAACGGGGGCGTAAGGACTCGACCCACGCAAGTGGGCCGAGTGGTCGCCCTTGGCCTTGGGTTGAGGCAGACCATATGGGTTGGGCCAAGGCCCGATCCCTCCGACCTTGGCCCGACCCTCTGGCCTGTTTCTCCTCCGGAGCCTGTTTCCTGGTTGTCTTCTTGGGCTGGTGCTACGTTGTCCTGCTTAGTCCTTGGATACCGGGGTGTTCACCAAGTTTTCCAGCACAGAGGCCTTGGACTTTCGAGACGAAGACCAAAATCAACCGATACTCTCCTCCAAAGGGACTCTGCAATTCCAGATCTTGCTTCCAAGGGTTCTGTTTCCATGGCGAAGCACAGGCAATCTCGTTTTCCTTCTCGGAAGTCCTCTTCTTCTTCTACTCTCGTCTTTACCCTGCTTATTATGTTCACCTTCGTCATTCTCATTCTTCTTGCCCTTGGAATCCTCTCGATCCCCGGCAATTCCGGCGGTTCGCCCAAGGTTCATGATCTGAGCTCGATTGTGCGCAAAACTTCGGACGATGTTGACGAGGAGAAAGGGGAGCAGTGGGTCGAAGTGATCTCATGGGAACCTAGAGCATTCGTTTATCATAACTTTCTGGTATCGTTTCTTCCGATCTATGATGAGAGATTTGTGTTTTATACAAAGGAGGAATGTGAGTACTTAATCAGCCTTGCCAAGCCTCACATGCAAAAGTCTACCGTTGTTGACAGTGAAACTGGAAAGAGCAAAGATAGCAGAGTTCGCACTAGTTCTGGAACATTTTTGCCGAGAGGACGTGATAAAACTATTAGAACCATTGAGAAAAGGATTGCTGATTTCACCTTCATACCCGTAGAGCATGGAGAAGGACTTCAGGTTCTTCATTACGAAGTGGGACAGAAATATGAACCTCATTTTGATTACTTCCTTGATGACTACAATACAAAGAATGGAGGTCAACGTATAGCAACAGTGCTTATGTATCTCTCAGAAGTTGAAGAAGGAGGCGAGACAGTGTTCCCTGCTGCCAAAGGAAACTTTAGTTCTGTACCTTGGTGGAATGAGCTTTCAGATTGTGGGAAGAAAGGACTTTCTGTTAAACCGAAGAGGGGTGATGCGCTGCTTTTCTGGAGCATGAAGCCTGATGCTTCTCTTGATCCATCAAGTTTGCATGGTGGTTGCCCTGTTATCAAGGGGAATAAATGGTCTGCTACTAAATGGATGCGAGTAGAAGAATACAAAGCTTGA

Coding sequence (CDS)

ATGGTTTGCGCGTCGGCAGAAGGTTCTTCTCCCGACGCAGTTAACGGCGTCGAGAGAAATCGCGTCGGCAAAAACAATTTTTCTTGTAGTGCGAACGGGGGCGTAAGGACTCGACCCACGCAAGTGGGCCGAGTGGTCGCCCTTGGCCTTGGGTTGAGGCAGACCATATGGGTTGGGCCAAGGCCCGATCCCTCCGACCTTGGCCCGACCCTCTGGCCTGTTTCTCCTCCGGAGCCTGTTTCCTGGTTGTCTTCTTGGGCTGGTGCTACGTTGTCCTGCTTAGTCCTTGGATACCGGGGTGTTCACCAAGTTTTCCAGCACAGAGGCCTTGGACTTTCGAGACGAAGACCAAAATCAACCGATACTCTCCTCCAAAGGGACTCTGCAATTCCAGATCTTGCTTCCAAGGGTTCTGTTTCCATGGCGAAGCACAGGCAATCTCGTTTTCCTTCTCGGAAGTCCTCTTCTTCTTCTACTCTCGTCTTTACCCTGCTTATTATGTTCACCTTCGTCATTCTCATTCTTCTTGCCCTTGGAATCCTCTCGATCCCCGGCAATTCCGGCGGTTCGCCCAAGGTTCATGATCTGAGCTCGATTGTGCGCAAAACTTCGGACGATGTTGACGAGGAGAAAGGGGAGCAGTGGGTCGAAGTGATCTCATGGGAACCTAGAGCATTCGTTTATCATAACTTTCTGGTATCGTTTCTTCCGATCTATGATGAGAGATTTGTGTTTTATACAAAGGAGGAATGTGAGTACTTAATCAGCCTTGCCAAGCCTCACATGCAAAAGTCTACCGTTGTTGACAGTGAAACTGGAAAGAGCAAAGATAGCAGAGTTCGCACTAGTTCTGGAACATTTTTGCCGAGAGGACGTGATAAAACTATTAGAACCATTGAGAAAAGGATTGCTGATTTCACCTTCATACCCGTAGAGCATGGAGAAGGACTTCAGGTTCTTCATTACGAAGTGGGACAGAAATATGAACCTCATTTTGATTACTTCCTTGATGACTACAATACAAAGAATGGAGGTCAACGTATAGCAACAGTGCTTATGTATCTCTCAGAAGTTGAAGAAGGAGGCGAGACAGTGTTCCCTGCTGCCAAAGGAAACTTTAGTTCTGTACCTTGGTGGAATGAGCTTTCAGATTGTGGGAAGAAAGGACTTTCTGTTAAACCGAAGAGGGGTGATGCGCTGCTTTTCTGGAGCATGAAGCCTGATGCTTCTCTTGATCCATCAAGTTTGCATGGTGGTTGCCCTGTTATCAAGGGGAATAAATGGTCTGCTACTAAATGGATGCGAGTAGAAGAATACAAAGCTTGA

Protein sequence

MVCASAEGSSPDAVNGVERNRVGKNNFSCSANGGVRTRPTQVGRVVALGLGLRQTIWVGPRPDPSDLGPTLWPVSPPEPVSWLSSWAGATLSCLVLGYRGVHQVFQHRGLGLSRRRPKSTDTLLQRDSAIPDLASKGSVSMAKHRQSRFPSRKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGSPKVHDLSSIVRKTSDDVDEEKGEQWVEVISWEPRAFVYHNFLVSFLPIYDERFVFYTKEECEYLISLAKPHMQKSTVVDSETGKSKDSRVRTSSGTFLPRGRDKTIRTIEKRIADFTFIPVEHGEGLQVLHYEVGQKYEPHFDYFLDDYNTKNGGQRIATVLMYLSEVEEGGETVFPAAKGNFSSVPWWNELSDCGKKGLSVKPKRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYKA
Homology
[Close]
BLAST of Spg012550 vs. NCBI nr
Match: XP_038881910.1 (probable prolyl 4-hydroxylase 10 isoform X2 [Benincasa hispida])

HSP 1 Score: 563.1 bits (1450), Expect = 2.0e-156
Identity = 280/300 (93.33%), Postives = 285/300 (95.00%), Query Frame = 0 

Query: 141 MAKHRQSRFPSRKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGSPKVHDLSSIV 200
           MAKHRQSRFP+RKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGSPKVHDLSSIV
Sbjct: 1   MAKHRQSRFPTRKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGSPKVHDLSSIV 60

Query: 201 RKTSDDVDEEKGEQWVEVISWEPRAFVYHNFLVSFLPIYDERFVFYTKEECEYLISLAKP 260
           RKTSDDVDEE+GEQWVEVISWEPRAFVYHNFL              TKEECEYLISLA P
Sbjct: 61  RKTSDDVDEEQGEQWVEVISWEPRAFVYHNFL--------------TKEECEYLISLAMP 120

Query: 261 HMQKSTVVDSETGKSKDSRVRTSSGTFLPRGRDKTIRTIEKRIADFTFIPVEHGEGLQVL 320
           HMQKSTVVDSETGKSKDSRVRTSSGTFLPRGRDKTIRTIEKRIADF+FIPVEHGEGLQVL
Sbjct: 121 HMQKSTVVDSETGKSKDSRVRTSSGTFLPRGRDKTIRTIEKRIADFSFIPVEHGEGLQVL 180

Query: 321 HYEVGQKYEPHFDYFLDDYNTKNGGQRIATVLMYLSEVEEGGETVFPAAKGNFSSVPWWN 380
           HYEVGQKYEPHFDYFLD+YNTKNGGQRIATVLMYLSEVEEGGETVFPAAKGNFSSVPWWN
Sbjct: 181 HYEVGQKYEPHFDYFLDEYNTKNGGQRIATVLMYLSEVEEGGETVFPAAKGNFSSVPWWN 240

Query: 381 ELSDCGKKGLSVKPKRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYK 440
           ELS+CGKKGLSVKPKRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYK
Sbjct: 241 ELSECGKKGLSVKPKRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYK 286

[Close]
BLAST of Spg012550 vs. NCBI nr
Match: XP_008440878.1 (PREDICTED: probable prolyl 4-hydroxylase 10 [Cucumis melo])

HSP 1 Score: 562.8 bits (1449), Expect = 2.7e-156
Identity = 279/301 (92.69%), Postives = 286/301 (95.02%), Query Frame = 0 

Query: 141 MAKHRQSRFPSRKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGSPKVHDLSSIV 200
           MAKHRQSRFP+RKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGS KVHDLSSIV
Sbjct: 1   MAKHRQSRFPTRKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGSTKVHDLSSIV 60

Query: 201 RKTSDDVDEEKGEQWVEVISWEPRAFVYHNFLVSFLPIYDERFVFYTKEECEYLISLAKP 260
           RKTSDDVDEEKGEQWVEVISWEPRAF+YHNFL              TKEECEYLISLAKP
Sbjct: 61  RKTSDDVDEEKGEQWVEVISWEPRAFIYHNFL--------------TKEECEYLISLAKP 120

Query: 261 HMQKSTVVDSETGKSKDSRVRTSSGTFLPRGRDKTIRTIEKRIADFTFIPVEHGEGLQVL 320
           HMQKSTVVDSETG+SKDSRVRTSSGTFLPRGRDKTIRTIEKRI+DF+FIPVEHGEGLQVL
Sbjct: 121 HMQKSTVVDSETGQSKDSRVRTSSGTFLPRGRDKTIRTIEKRISDFSFIPVEHGEGLQVL 180

Query: 321 HYEVGQKYEPHFDYFLDDYNTKNGGQRIATVLMYLSEVEEGGETVFPAAKGNFSSVPWWN 380
           HYEVGQKYEPHFDYFLD+YNTKNGGQRIATVLMYLS+VEEGGETVFPAAKGNFSSVPWWN
Sbjct: 181 HYEVGQKYEPHFDYFLDEYNTKNGGQRIATVLMYLSDVEEGGETVFPAAKGNFSSVPWWN 240

Query: 381 ELSDCGKKGLSVKPKRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYK 440
           ELSDCGKKGLSVKPKRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYK
Sbjct: 241 ELSDCGKKGLSVKPKRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYK 287

Query: 441 A 442
           A
Sbjct: 301 A 287

[Close]
BLAST of Spg012550 vs. NCBI nr
Match: XP_004134841.1 (probable prolyl 4-hydroxylase 10 [Cucumis sativus] >XP_031742781.1 probable prolyl 4-hydroxylase 10 [Cucumis sativus] >KGN48959.1 hypothetical protein Csa_003630 [Cucumis sativus])

HSP 1 Score: 562.0 bits (1447), Expect = 4.6e-156
Identity = 278/301 (92.36%), Postives = 286/301 (95.02%), Query Frame = 0 

Query: 141 MAKHRQSRFPSRKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGSPKVHDLSSIV 200
           MAKHRQSRFP+RKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGS KVHDLSSIV
Sbjct: 1   MAKHRQSRFPTRKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGSTKVHDLSSIV 60

Query: 201 RKTSDDVDEEKGEQWVEVISWEPRAFVYHNFLVSFLPIYDERFVFYTKEECEYLISLAKP 260
           RKTSDDVDEEKGEQWVEVISWEPRAFVYHNFL              TKEECEYLISLAKP
Sbjct: 61  RKTSDDVDEEKGEQWVEVISWEPRAFVYHNFL--------------TKEECEYLISLAKP 120

Query: 261 HMQKSTVVDSETGKSKDSRVRTSSGTFLPRGRDKTIRTIEKRIADFTFIPVEHGEGLQVL 320
           HMQKSTVVDSETG+SKDSRVRTSSGTFLPRGRDKT+RTIEKR++DF+FIPVEHGEGLQVL
Sbjct: 121 HMQKSTVVDSETGQSKDSRVRTSSGTFLPRGRDKTVRTIEKRLSDFSFIPVEHGEGLQVL 180

Query: 321 HYEVGQKYEPHFDYFLDDYNTKNGGQRIATVLMYLSEVEEGGETVFPAAKGNFSSVPWWN 380
           HYEVGQKYEPHFDYFLD+YNTKNGGQRIATVLMYLS+VEEGGETVFPAAKGNFSSVPWWN
Sbjct: 181 HYEVGQKYEPHFDYFLDEYNTKNGGQRIATVLMYLSDVEEGGETVFPAAKGNFSSVPWWN 240

Query: 381 ELSDCGKKGLSVKPKRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYK 440
           ELSDCGKKGLSVKPKRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYK
Sbjct: 241 ELSDCGKKGLSVKPKRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYK 287

Query: 441 A 442
           A
Sbjct: 301 A 287

[Close]
BLAST of Spg012550 vs. NCBI nr
Match: XP_038881909.1 (probable prolyl 4-hydroxylase 10 isoform X1 [Benincasa hispida])

HSP 1 Score: 555.8 bits (1431), Expect = 3.3e-154
Identity = 279/304 (91.78%), Postives = 285/304 (93.75%), Query Frame = 0 

Query: 141 MAKHRQSRFPSRKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGSPKVHDLSSIV 200
           MAKHRQSRFP+RKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGSPKVHDLSSIV
Sbjct: 1   MAKHRQSRFPTRKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGSPKVHDLSSIV 60

Query: 201 RKTSDD----VDEEKGEQWVEVISWEPRAFVYHNFLVSFLPIYDERFVFYTKEECEYLIS 260
           RKTSD+    VDEE+GEQWVEVISWEPRAFVYHNFL              TKEECEYLIS
Sbjct: 61  RKTSDEYVISVDEEQGEQWVEVISWEPRAFVYHNFL--------------TKEECEYLIS 120

Query: 261 LAKPHMQKSTVVDSETGKSKDSRVRTSSGTFLPRGRDKTIRTIEKRIADFTFIPVEHGEG 320
           LA PHMQKSTVVDSETGKSKDSRVRTSSGTFLPRGRDKTIRTIEKRIADF+FIPVEHGEG
Sbjct: 121 LAMPHMQKSTVVDSETGKSKDSRVRTSSGTFLPRGRDKTIRTIEKRIADFSFIPVEHGEG 180

Query: 321 LQVLHYEVGQKYEPHFDYFLDDYNTKNGGQRIATVLMYLSEVEEGGETVFPAAKGNFSSV 380
           LQVLHYEVGQKYEPHFDYFLD+YNTKNGGQRIATVLMYLSEVEEGGETVFPAAKGNFSSV
Sbjct: 181 LQVLHYEVGQKYEPHFDYFLDEYNTKNGGQRIATVLMYLSEVEEGGETVFPAAKGNFSSV 240

Query: 381 PWWNELSDCGKKGLSVKPKRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRV 440
           PWWNELS+CGKKGLSVKPKRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRV
Sbjct: 241 PWWNELSECGKKGLSVKPKRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRV 290

[Close]
BLAST of Spg012550 vs. NCBI nr
Match: XP_022978860.1 (probable prolyl 4-hydroxylase 10 [Cucurbita maxima])

HSP 1 Score: 554.7 bits (1428), Expect = 7.3e-154
Identity = 274/301 (91.03%), Postives = 282/301 (93.69%), Query Frame = 0 

Query: 141 MAKHRQSRFPSRKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGSPKVHDLSSIV 200
           MAKHRQSRFP+RKSSSSST++FTLLIMFTFVILILLALGILSIPGNSGGSPKVHDLSSIV
Sbjct: 1   MAKHRQSRFPTRKSSSSSTIIFTLLIMFTFVILILLALGILSIPGNSGGSPKVHDLSSIV 60

Query: 201 RKTSDDVDEEKGEQWVEVISWEPRAFVYHNFLVSFLPIYDERFVFYTKEECEYLISLAKP 260
           RKTS+DVDEEKGE+W EVISWEPRAFVYHNFL              TKEECEYLIS AKP
Sbjct: 61  RKTSEDVDEEKGERWAEVISWEPRAFVYHNFL--------------TKEECEYLISHAKP 120

Query: 261 HMQKSTVVDSETGKSKDSRVRTSSGTFLPRGRDKTIRTIEKRIADFTFIPVEHGEGLQVL 320
           HMQKSTVVDSETGKSKDSRVRTSSGTFLPRG DK I TIEKRIADFTFIP+EHGEGLQVL
Sbjct: 121 HMQKSTVVDSETGKSKDSRVRTSSGTFLPRGGDKIISTIEKRIADFTFIPIEHGEGLQVL 180

Query: 321 HYEVGQKYEPHFDYFLDDYNTKNGGQRIATVLMYLSEVEEGGETVFPAAKGNFSSVPWWN 380
           HYEVGQKYEPHFDYFLDDYNTKNGGQRIATVLMYLS+VEEGGETVFPAAKGNFSSVPWWN
Sbjct: 181 HYEVGQKYEPHFDYFLDDYNTKNGGQRIATVLMYLSDVEEGGETVFPAAKGNFSSVPWWN 240

Query: 381 ELSDCGKKGLSVKPKRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYK 440
           ELSDCGKKGLSVKPKRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRV+EYK
Sbjct: 241 ELSDCGKKGLSVKPKRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVDEYK 287

Query: 441 A 442
           A
Sbjct: 301 A 287

[Close]
BLAST of Spg012550 vs. ExPASy Swiss-Prot
Match: F4JZ24 (Probable prolyl 4-hydroxylase 10 OS=Arabidopsis thaliana OX=3702 GN=P4H10 PE=3 SV=1)

HSP 1 Score: 461.1 bits (1185), Expect = 1.4e-128
Identity = 229/302 (75.83%), Postives = 255/302 (84.44%), Query Frame = 0 

Query: 141 MAKHRQSRFPSRKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGSPKVHDLSSIV 200
           MA+ R  R PS + SS STLVF +LIM TFVILILLA GILS+P N+ GS K +DL+SIV
Sbjct: 2   MARPRNHR-PSARKSSHSTLVFAVLIMSTFVILILLAFGILSVPSNNAGSSKANDLTSIV 61

Query: 201 RKT--SDDVDEEKGEQWVEVISWEPRAFVYHNFLVSFLPIYDERFVFYTKEECEYLISLA 260
           RKT      D+ K E+WVE+ISWEPRA VYHNFL              TKEEC+YLI LA
Sbjct: 62  RKTLQRSGEDDSKNERWVEIISWEPRASVYHNFL--------------TKEECKYLIELA 121

Query: 261 KPHMQKSTVVDSETGKSKDSRVRTSSGTFLPRGRDKTIRTIEKRIADFTFIPVEHGEGLQ 320
           KPHM+KSTVVD +TGKS DSRVRTSSGTFL RGRDKTIR IEKRI+DFTFIPVEHGEGLQ
Sbjct: 122 KPHMEKSTVVDEKTGKSTDSRVRTSSGTFLARGRDKTIREIEKRISDFTFIPVEHGEGLQ 181

Query: 321 VLHYEVGQKYEPHFDYFLDDYNTKNGGQRIATVLMYLSEVEEGGETVFPAAKGNFSSVPW 380
           VLHYE+GQKYEPH+DYF+D+YNT+NGGQRIATVLMYLS+VEEGGETVFPAAKGN+S+VPW
Sbjct: 182 VLHYEIGQKYEPHYDYFMDEYNTRNGGQRIATVLMYLSDVEEGGETVFPAAKGNYSAVPW 241

Query: 381 WNELSDCGKKGLSVKPKRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEE 440
           WNELS+CGK GLSVKPK GDALLFWSM PDA+LDPSSLHGGC VIKGNKWS+TKW+RV E
Sbjct: 242 WNELSECGKGGLSVKPKMGDALLFWSMTPDATLDPSSLHGGCAVIKGNKWSSTKWLRVHE 288

[Close]
BLAST of Spg012550 vs. ExPASy Swiss-Prot
Match: Q9LN20 (Probable prolyl 4-hydroxylase 3 OS=Arabidopsis thaliana OX=3702 GN=P4H3 PE=2 SV=1)

HSP 1 Score: 421.4 bits (1082), Expect = 1.3e-116
Identity = 211/302 (69.87%), Postives = 248/302 (82.12%), Query Frame = 0 

Query: 141 MAKHRQSRFPSRKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGSPKVHDLSSIV 200
           MAK R SRF +RK S+   ++F +L M T V+L+LLA G+ S+P N+  S  + DLS   
Sbjct: 1   MAKLRHSRFQARKWSTLMLVLF-MLFMLTIVLLMLLAFGVFSLPINNDESSPI-DLSYFR 60

Query: 201 RKTSDDVD--EEKGEQWVEVISWEPRAFVYHNFLVSFLPIYDERFVFYTKEECEYLISLA 260
           R  ++  +   ++G+QW EV+SWEPRAFVYHNFL              +KEECEYLISLA
Sbjct: 61  RAATERSEGLGKRGDQWTEVLSWEPRAFVYHNFL--------------SKEECEYLISLA 120

Query: 261 KPHMQKSTVVDSETGKSKDSRVRTSSGTFLPRGRDKTIRTIEKRIADFTFIPVEHGEGLQ 320
           KPHM KSTVVDSETGKSKDSRVRTSSGTFL RGRDK I+TIEKRIAD+TFIP +HGEGLQ
Sbjct: 121 KPHMVKSTVVDSETGKSKDSRVRTSSGTFLRRGRDKIIKTIEKRIADYTFIPADHGEGLQ 180

Query: 321 VLHYEVGQKYEPHFDYFLDDYNTKNGGQRIATVLMYLSEVEEGGETVFPAAKGNFSSVPW 380
           VLHYE GQKYEPH+DYF+D++NTKNGGQR+AT+LMYLS+VEEGGETVFPAA  NFSSVPW
Sbjct: 181 VLHYEAGQKYEPHYDYFVDEFNTKNGGQRMATMLMYLSDVEEGGETVFPAANMNFSSVPW 240

Query: 381 WNELSDCGKKGLSVKPKRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEE 440
           +NELS+CGKKGLSVKP+ GDALLFWSM+PDA+LDP+SLHGGCPVI+GNKWS+TKWM V E
Sbjct: 241 YNELSECGKKGLSVKPRMGDALLFWSMRPDATLDPTSLHGGCPVIRGNKWSSTKWMHVGE 286

[Close]
BLAST of Spg012550 vs. ExPASy Swiss-Prot
Match: Q24JN5 (Prolyl 4-hydroxylase 5 OS=Arabidopsis thaliana OX=3702 GN=P4H5 PE=2 SV=1)

HSP 1 Score: 419.9 bits (1078), Expect = 3.7e-116
Identity = 208/295 (70.51%), Postives = 240/295 (81.36%), Query Frame = 0 

Query: 148 RFPSRKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGSPKVHDLSSIVRK--TSD 207
           R+  RKS S ST  FT+LI+   VILILL LGILS+P  +  S K +DL++IVRK  TS 
Sbjct: 10  RYQPRKSVSRSTQAFTVLILLLVVILILLGLGILSLPNANRNSSKTNDLTNIVRKSETSS 69

Query: 208 DVDEEKGEQWVEVISWEPRAFVYHNFLVSFLPIYDERFVFYTKEECEYLISLAKPHMQKS 267
             +E  GE+WVEVISWEPRA VYHNFL              T EECE+LISLAKP M KS
Sbjct: 70  GDEEGNGERWVEVISWEPRAVVYHNFL--------------TNEECEHLISLAKPSMVKS 129

Query: 268 TVVDSETGKSKDSRVRTSSGTFLPRGRDKTIRTIEKRIADFTFIPVEHGEGLQVLHYEVG 327
           TVVD +TG SKDSRVRTSSGTFL RG D+ +  IEKRI+DFTFIPVE+GEGLQVLHY+VG
Sbjct: 130 TVVDEKTGGSKDSRVRTSSGTFLRRGHDEVVEVIEKRISDFTFIPVENGEGLQVLHYQVG 189

Query: 328 QKYEPHFDYFLDDYNTKNGGQRIATVLMYLSEVEEGGETVFPAAKGNFSSVPWWNELSDC 387
           QKYEPH+DYFLD++NTKNGGQRIATVLMYLS+V++GGETVFPAA+GN S+VPWWNELS C
Sbjct: 190 QKYEPHYDYFLDEFNTKNGGQRIATVLMYLSDVDDGGETVFPAARGNISAVPWWNELSKC 249

Query: 388 GKKGLSVKPKRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYK 441
           GK+GLSV PK+ DALLFW+M+PDASLDPSSLHGGCPV+KGNKWS+TKW  V E+K
Sbjct: 250 GKEGLSVLPKKRDALLFWNMRPDASLDPSSLHGGCPVVKGNKWSSTKWFHVHEFK 290

[Close]
BLAST of Spg012550 vs. ExPASy Swiss-Prot
Match: F4JNU8 (Probable prolyl 4-hydroxylase 8 OS=Arabidopsis thaliana OX=3702 GN=P4H8 PE=3 SV=1)

HSP 1 Score: 391.3 bits (1004), Expect = 1.4e-107
Identity = 198/302 (65.56%), Postives = 232/302 (76.82%), Query Frame = 0 

Query: 143 KHRQSRFPSRKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGSPKVHDLSSIV-- 202
           K +Q R   RKS S+ T  FT++++  FVILIL+ LGI S+P  +  S    DL++IV  
Sbjct: 4   KPKQLRNKPRKSFSTQT--FTVVVLVLFVILILVGLGIFSLPSTNKTSSMPMDLTTIVQT 63

Query: 203 ---RKTSDDVDEEKGEQWVEVISWEPRAFVYHNFLVSFLPIYDERFVFYTKEECEYLISL 262
              R++  D ++  G++W+EVISWEPRAFVYHNFL              T EECE+LISL
Sbjct: 64  IQERESFGDEEDGNGDRWLEVISWEPRAFVYHNFL--------------TNEECEHLISL 123

Query: 263 AKPHMQKSTVVDSETGKSKDSRVRTSSGTFLPRGRDKTIRTIEKRIADFTFIPVEHGEGL 322
           AKP M KS VVD +TGKS DSRVRTSSGTFL RG D+ +  IE RI+DFTFIP E+GEGL
Sbjct: 124 AKPSMMKSKVVDVKTGKSIDSRVRTSSGTFLNRGHDEIVEEIENRISDFTFIPPENGEGL 183

Query: 323 QVLHYEVGQKYEPHFDYFLDDYNTKNGGQRIATVLMYLSEVEEGGETVFPAAKGNFSSVP 382
           QVLHYEVGQ+YEPH DYF D++N + GGQRIATVLMYLS+V+EGGETVFPAAKGN S VP
Sbjct: 184 QVLHYEVGQRYEPHHDYFFDEFNVRKGGQRIATVLMYLSDVDEGGETVFPAAKGNVSDVP 243

Query: 383 WWNELSDCGKKGLSVKPKRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVE 440
           WW+ELS CGK+GLSV PK+ DALLFWSMKPDASLDPSSLHGGCPVIKGNKWS+TKW  V 
Sbjct: 244 WWDELSQCGKEGLSVLPKKRDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSSTKWFHVH 289

[Close]
BLAST of Spg012550 vs. ExPASy Swiss-Prot
Match: Q8LAN3 (Probable prolyl 4-hydroxylase 4 OS=Arabidopsis thaliana OX=3702 GN=P4H4 PE=2 SV=1)

HSP 1 Score: 240.4 bits (612), Expect = 4.0e-62
Identity = 122/245 (49.80%), Postives = 170/245 (69.39%), Query Frame = 0 

Query: 197 SSIVRKTSDDVDEEKGEQWVEVISWEPRAFVYHNFLVSFLPIYDERFVFYTKEECEYLIS 256
           +S++  +S  V+  K    V+ +S +PRAFVY  FL              T+ EC++++S
Sbjct: 22  TSLISSSSVFVNPSK----VKQVSSKPRAFVYEGFL--------------TELECDHMVS 81

Query: 257 LAKPHMQKSTVVDSETGKSKDSRVRTSSGTFLPRGRDKTIRTIEKRIADFTFIPVEHGEG 316
           LAK  +++S V D+++G+SK S VRTSSGTF+ +G+D  +  IE +I+ +TF+P E+GE 
Sbjct: 82  LAKASLKRSAVADNDSGESKFSEVRTSSGTFISKGKDPIVSGIEDKISTWTFLPKENGED 141

Query: 317 LQVLHYEVGQKYEPHFDYFLDDYNTKNGGQRIATVLMYLSEVEEGGETVFPAAKGNFSSV 376
           +QVL YE GQKY+ HFDYF D  N   GG R+AT+LMYLS V +GGETVFP A+     V
Sbjct: 142 IQVLRYEHGQKYDAHFDYFHDKVNIVRGGHRMATILMYLSNVTKGGETVFPDAEIPSRRV 201

Query: 377 PWWN--ELSDCGKKGLSVKPKRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWM 436
              N  +LSDC K+G++VKP++GDALLF+++ PDA  DP SLHGGCPVI+G KWSATKW+
Sbjct: 202 LSENKEDLSDCAKRGIAVKPRKGDALLFFNLHPDAIPDPLSLHGGCPVIEGEKWSATKWI 248

Query: 437 RVEEY 440
            V+ +
Sbjct: 262 HVDSF 248

[Close]
BLAST of Spg012550 vs. ExPASy TrEMBL
Match: A0A1S3B1P6 (probable prolyl 4-hydroxylase 10 OS=Cucumis melo OX=3656 GN=LOC103485167 PE=4 SV=1)

HSP 1 Score: 562.8 bits (1449), Expect = 1.3e-156
Identity = 279/301 (92.69%), Postives = 286/301 (95.02%), Query Frame = 0 

Query: 141 MAKHRQSRFPSRKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGSPKVHDLSSIV 200
           MAKHRQSRFP+RKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGS KVHDLSSIV
Sbjct: 1   MAKHRQSRFPTRKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGSTKVHDLSSIV 60

Query: 201 RKTSDDVDEEKGEQWVEVISWEPRAFVYHNFLVSFLPIYDERFVFYTKEECEYLISLAKP 260
           RKTSDDVDEEKGEQWVEVISWEPRAF+YHNFL              TKEECEYLISLAKP
Sbjct: 61  RKTSDDVDEEKGEQWVEVISWEPRAFIYHNFL--------------TKEECEYLISLAKP 120

Query: 261 HMQKSTVVDSETGKSKDSRVRTSSGTFLPRGRDKTIRTIEKRIADFTFIPVEHGEGLQVL 320
           HMQKSTVVDSETG+SKDSRVRTSSGTFLPRGRDKTIRTIEKRI+DF+FIPVEHGEGLQVL
Sbjct: 121 HMQKSTVVDSETGQSKDSRVRTSSGTFLPRGRDKTIRTIEKRISDFSFIPVEHGEGLQVL 180

Query: 321 HYEVGQKYEPHFDYFLDDYNTKNGGQRIATVLMYLSEVEEGGETVFPAAKGNFSSVPWWN 380
           HYEVGQKYEPHFDYFLD+YNTKNGGQRIATVLMYLS+VEEGGETVFPAAKGNFSSVPWWN
Sbjct: 181 HYEVGQKYEPHFDYFLDEYNTKNGGQRIATVLMYLSDVEEGGETVFPAAKGNFSSVPWWN 240

Query: 381 ELSDCGKKGLSVKPKRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYK 440
           ELSDCGKKGLSVKPKRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYK
Sbjct: 241 ELSDCGKKGLSVKPKRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYK 287

Query: 441 A 442
           A
Sbjct: 301 A 287

[Close]
BLAST of Spg012550 vs. ExPASy TrEMBL
Match: A0A0A0KJH9 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G507320 PE=4 SV=1)

HSP 1 Score: 562.0 bits (1447), Expect = 2.2e-156
Identity = 278/301 (92.36%), Postives = 286/301 (95.02%), Query Frame = 0 

Query: 141 MAKHRQSRFPSRKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGSPKVHDLSSIV 200
           MAKHRQSRFP+RKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGS KVHDLSSIV
Sbjct: 1   MAKHRQSRFPTRKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGSTKVHDLSSIV 60

Query: 201 RKTSDDVDEEKGEQWVEVISWEPRAFVYHNFLVSFLPIYDERFVFYTKEECEYLISLAKP 260
           RKTSDDVDEEKGEQWVEVISWEPRAFVYHNFL              TKEECEYLISLAKP
Sbjct: 61  RKTSDDVDEEKGEQWVEVISWEPRAFVYHNFL--------------TKEECEYLISLAKP 120

Query: 261 HMQKSTVVDSETGKSKDSRVRTSSGTFLPRGRDKTIRTIEKRIADFTFIPVEHGEGLQVL 320
           HMQKSTVVDSETG+SKDSRVRTSSGTFLPRGRDKT+RTIEKR++DF+FIPVEHGEGLQVL
Sbjct: 121 HMQKSTVVDSETGQSKDSRVRTSSGTFLPRGRDKTVRTIEKRLSDFSFIPVEHGEGLQVL 180

Query: 321 HYEVGQKYEPHFDYFLDDYNTKNGGQRIATVLMYLSEVEEGGETVFPAAKGNFSSVPWWN 380
           HYEVGQKYEPHFDYFLD+YNTKNGGQRIATVLMYLS+VEEGGETVFPAAKGNFSSVPWWN
Sbjct: 181 HYEVGQKYEPHFDYFLDEYNTKNGGQRIATVLMYLSDVEEGGETVFPAAKGNFSSVPWWN 240

Query: 381 ELSDCGKKGLSVKPKRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYK 440
           ELSDCGKKGLSVKPKRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYK
Sbjct: 241 ELSDCGKKGLSVKPKRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYK 287

Query: 441 A 442
           A
Sbjct: 301 A 287

[Close]
BLAST of Spg012550 vs. ExPASy TrEMBL
Match: A0A6J1IRF1 (probable prolyl 4-hydroxylase 10 OS=Cucurbita maxima OX=3661 GN=LOC111478689 PE=4 SV=1)

HSP 1 Score: 554.7 bits (1428), Expect = 3.5e-154
Identity = 274/301 (91.03%), Postives = 282/301 (93.69%), Query Frame = 0 

Query: 141 MAKHRQSRFPSRKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGSPKVHDLSSIV 200
           MAKHRQSRFP+RKSSSSST++FTLLIMFTFVILILLALGILSIPGNSGGSPKVHDLSSIV
Sbjct: 1   MAKHRQSRFPTRKSSSSSTIIFTLLIMFTFVILILLALGILSIPGNSGGSPKVHDLSSIV 60

Query: 201 RKTSDDVDEEKGEQWVEVISWEPRAFVYHNFLVSFLPIYDERFVFYTKEECEYLISLAKP 260
           RKTS+DVDEEKGE+W EVISWEPRAFVYHNFL              TKEECEYLIS AKP
Sbjct: 61  RKTSEDVDEEKGERWAEVISWEPRAFVYHNFL--------------TKEECEYLISHAKP 120

Query: 261 HMQKSTVVDSETGKSKDSRVRTSSGTFLPRGRDKTIRTIEKRIADFTFIPVEHGEGLQVL 320
           HMQKSTVVDSETGKSKDSRVRTSSGTFLPRG DK I TIEKRIADFTFIP+EHGEGLQVL
Sbjct: 121 HMQKSTVVDSETGKSKDSRVRTSSGTFLPRGGDKIISTIEKRIADFTFIPIEHGEGLQVL 180

Query: 321 HYEVGQKYEPHFDYFLDDYNTKNGGQRIATVLMYLSEVEEGGETVFPAAKGNFSSVPWWN 380
           HYEVGQKYEPHFDYFLDDYNTKNGGQRIATVLMYLS+VEEGGETVFPAAKGNFSSVPWWN
Sbjct: 181 HYEVGQKYEPHFDYFLDDYNTKNGGQRIATVLMYLSDVEEGGETVFPAAKGNFSSVPWWN 240

Query: 381 ELSDCGKKGLSVKPKRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYK 440
           ELSDCGKKGLSVKPKRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRV+EYK
Sbjct: 241 ELSDCGKKGLSVKPKRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVDEYK 287

Query: 441 A 442
           A
Sbjct: 301 A 287

[Close]
BLAST of Spg012550 vs. ExPASy TrEMBL
Match: A0A6J1GDV6 (probable prolyl 4-hydroxylase 10 OS=Cucurbita moschata OX=3662 GN=LOC111453285 PE=4 SV=1)

HSP 1 Score: 553.1 bits (1424), Expect = 1.0e-153
Identity = 274/301 (91.03%), Postives = 281/301 (93.36%), Query Frame = 0 

Query: 141 MAKHRQSRFPSRKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGSPKVHDLSSIV 200
           MAKHRQSR P+RKSSSSSTL+FTLLIMFTFVILILLALGILSIPGNSGGSPKVHDLSSIV
Sbjct: 1   MAKHRQSRLPTRKSSSSSTLIFTLLIMFTFVILILLALGILSIPGNSGGSPKVHDLSSIV 60

Query: 201 RKTSDDVDEEKGEQWVEVISWEPRAFVYHNFLVSFLPIYDERFVFYTKEECEYLISLAKP 260
           RKTS+DVDEEKGE+W EVISWEPRAFVYHNFL              TKEECEYLIS AKP
Sbjct: 61  RKTSEDVDEEKGERWAEVISWEPRAFVYHNFL--------------TKEECEYLISHAKP 120

Query: 261 HMQKSTVVDSETGKSKDSRVRTSSGTFLPRGRDKTIRTIEKRIADFTFIPVEHGEGLQVL 320
           HMQKSTVVDSETGKSKDSRVRTSSGTFLPRG DK I TIEKRIADFTFIP+EHGEGLQVL
Sbjct: 121 HMQKSTVVDSETGKSKDSRVRTSSGTFLPRGGDKIISTIEKRIADFTFIPIEHGEGLQVL 180

Query: 321 HYEVGQKYEPHFDYFLDDYNTKNGGQRIATVLMYLSEVEEGGETVFPAAKGNFSSVPWWN 380
           HYEVGQKYEPHFDYFLDDYNTKNGGQRIATVLMYLS+VEEGGETVFPAAKGNFSSVPWWN
Sbjct: 181 HYEVGQKYEPHFDYFLDDYNTKNGGQRIATVLMYLSDVEEGGETVFPAAKGNFSSVPWWN 240

Query: 381 ELSDCGKKGLSVKPKRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYK 440
           ELSDCGKKGLSVKPKRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRV+EYK
Sbjct: 241 ELSDCGKKGLSVKPKRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVDEYK 287

Query: 441 A 442
           A
Sbjct: 301 A 287

[Close]
BLAST of Spg012550 vs. ExPASy TrEMBL
Match: A0A6J1BWA6 (probable prolyl 4-hydroxylase 10 OS=Momordica charantia OX=3673 GN=LOC111005915 PE=4 SV=1)

HSP 1 Score: 549.3 bits (1414), Expect = 1.5e-152
Identity = 271/301 (90.03%), Postives = 281/301 (93.36%), Query Frame = 0 

Query: 141 MAKHRQSRFPSRKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGSPKVHDLSSIV 200
           MAKHRQSRFP+RKSSSSSTL+FTLLIMFTFVILILLALGILSIPGNSG SPK HDLSSIV
Sbjct: 1   MAKHRQSRFPARKSSSSSTLIFTLLIMFTFVILILLALGILSIPGNSGDSPKAHDLSSIV 60

Query: 201 RKTSDDVDEEKGEQWVEVISWEPRAFVYHNFLVSFLPIYDERFVFYTKEECEYLISLAKP 260
           RKTSDD++EEKGEQWVEVISWEPRAFVYHNFL              TKEECEYL+SLAKP
Sbjct: 61  RKTSDDIEEEKGEQWVEVISWEPRAFVYHNFL--------------TKEECEYLVSLAKP 120

Query: 261 HMQKSTVVDSETGKSKDSRVRTSSGTFLPRGRDKTIRTIEKRIADFTFIPVEHGEGLQVL 320
           +MQKSTVVDSETGKSKDSRVRTSSGTFLPRGRDKTIR IEKRIADF+FIP+EHGEGLQVL
Sbjct: 121 NMQKSTVVDSETGKSKDSRVRTSSGTFLPRGRDKTIRNIEKRIADFSFIPMEHGEGLQVL 180

Query: 321 HYEVGQKYEPHFDYFLDDYNTKNGGQRIATVLMYLSEVEEGGETVFPAAKGNFSSVPWWN 380
           HYEVGQKYEPHFDYFLDD+NTKNGGQRIATVLMYLSEVEEGGETVFPAAKGN SSVPWWN
Sbjct: 181 HYEVGQKYEPHFDYFLDDFNTKNGGQRIATVLMYLSEVEEGGETVFPAAKGNISSVPWWN 240

Query: 381 ELSDCGKKGLSVKPKRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYK 440
           ELS+CGKKGLSVKPK GDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRV EYK
Sbjct: 241 ELSECGKKGLSVKPKMGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVNEYK 287

Query: 441 A 442
           A
Sbjct: 301 A 287

[Close]
BLAST of Spg012550 vs. TAIR 10
Match: AT5G66060.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 461.1 bits (1185), Expect = 1.0e-129
Identity = 229/302 (75.83%), Postives = 255/302 (84.44%), Query Frame = 0 

Query: 141 MAKHRQSRFPSRKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGSPKVHDLSSIV 200
           MA+ R  R PS + SS STLVF +LIM TFVILILLA GILS+P N+ GS K +DL+SIV
Sbjct: 2   MARPRNHR-PSARKSSHSTLVFAVLIMSTFVILILLAFGILSVPSNNAGSSKANDLTSIV 61

Query: 201 RKT--SDDVDEEKGEQWVEVISWEPRAFVYHNFLVSFLPIYDERFVFYTKEECEYLISLA 260
           RKT      D+ K E+WVE+ISWEPRA VYHNFL              TKEEC+YLI LA
Sbjct: 62  RKTLQRSGEDDSKNERWVEIISWEPRASVYHNFL--------------TKEECKYLIELA 121

Query: 261 KPHMQKSTVVDSETGKSKDSRVRTSSGTFLPRGRDKTIRTIEKRIADFTFIPVEHGEGLQ 320
           KPHM+KSTVVD +TGKS DSRVRTSSGTFL RGRDKTIR IEKRI+DFTFIPVEHGEGLQ
Sbjct: 122 KPHMEKSTVVDEKTGKSTDSRVRTSSGTFLARGRDKTIREIEKRISDFTFIPVEHGEGLQ 181

Query: 321 VLHYEVGQKYEPHFDYFLDDYNTKNGGQRIATVLMYLSEVEEGGETVFPAAKGNFSSVPW 380
           VLHYE+GQKYEPH+DYF+D+YNT+NGGQRIATVLMYLS+VEEGGETVFPAAKGN+S+VPW
Sbjct: 182 VLHYEIGQKYEPHYDYFMDEYNTRNGGQRIATVLMYLSDVEEGGETVFPAAKGNYSAVPW 241

Query: 381 WNELSDCGKKGLSVKPKRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEE 440
           WNELS+CGK GLSVKPK GDALLFWSM PDA+LDPSSLHGGC VIKGNKWS+TKW+RV E
Sbjct: 242 WNELSECGKGGLSVKPKMGDALLFWSMTPDATLDPSSLHGGCAVIKGNKWSSTKWLRVHE 288

[Close]
BLAST of Spg012550 vs. TAIR 10
Match: AT1G20270.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 421.4 bits (1082), Expect = 9.0e-118
Identity = 211/302 (69.87%), Postives = 248/302 (82.12%), Query Frame = 0 

Query: 141 MAKHRQSRFPSRKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGSPKVHDLSSIV 200
           MAK R SRF +RK S+   ++F +L M T V+L+LLA G+ S+P N+  S  + DLS   
Sbjct: 1   MAKLRHSRFQARKWSTLMLVLF-MLFMLTIVLLMLLAFGVFSLPINNDESSPI-DLSYFR 60

Query: 201 RKTSDDVD--EEKGEQWVEVISWEPRAFVYHNFLVSFLPIYDERFVFYTKEECEYLISLA 260
           R  ++  +   ++G+QW EV+SWEPRAFVYHNFL              +KEECEYLISLA
Sbjct: 61  RAATERSEGLGKRGDQWTEVLSWEPRAFVYHNFL--------------SKEECEYLISLA 120

Query: 261 KPHMQKSTVVDSETGKSKDSRVRTSSGTFLPRGRDKTIRTIEKRIADFTFIPVEHGEGLQ 320
           KPHM KSTVVDSETGKSKDSRVRTSSGTFL RGRDK I+TIEKRIAD+TFIP +HGEGLQ
Sbjct: 121 KPHMVKSTVVDSETGKSKDSRVRTSSGTFLRRGRDKIIKTIEKRIADYTFIPADHGEGLQ 180

Query: 321 VLHYEVGQKYEPHFDYFLDDYNTKNGGQRIATVLMYLSEVEEGGETVFPAAKGNFSSVPW 380
           VLHYE GQKYEPH+DYF+D++NTKNGGQR+AT+LMYLS+VEEGGETVFPAA  NFSSVPW
Sbjct: 181 VLHYEAGQKYEPHYDYFVDEFNTKNGGQRMATMLMYLSDVEEGGETVFPAANMNFSSVPW 240

Query: 381 WNELSDCGKKGLSVKPKRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEE 440
           +NELS+CGKKGLSVKP+ GDALLFWSM+PDA+LDP+SLHGGCPVI+GNKWS+TKWM V E
Sbjct: 241 YNELSECGKKGLSVKPRMGDALLFWSMRPDATLDPTSLHGGCPVIRGNKWSSTKWMHVGE 286

[Close]
BLAST of Spg012550 vs. TAIR 10
Match: AT2G17720.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 419.9 bits (1078), Expect = 2.6e-117
Identity = 208/295 (70.51%), Postives = 240/295 (81.36%), Query Frame = 0 

Query: 148 RFPSRKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGSPKVHDLSSIVRK--TSD 207
           R+  RKS S ST  FT+LI+   VILILL LGILS+P  +  S K +DL++IVRK  TS 
Sbjct: 10  RYQPRKSVSRSTQAFTVLILLLVVILILLGLGILSLPNANRNSSKTNDLTNIVRKSETSS 69

Query: 208 DVDEEKGEQWVEVISWEPRAFVYHNFLVSFLPIYDERFVFYTKEECEYLISLAKPHMQKS 267
             +E  GE+WVEVISWEPRA VYHNFL              T EECE+LISLAKP M KS
Sbjct: 70  GDEEGNGERWVEVISWEPRAVVYHNFL--------------TNEECEHLISLAKPSMVKS 129

Query: 268 TVVDSETGKSKDSRVRTSSGTFLPRGRDKTIRTIEKRIADFTFIPVEHGEGLQVLHYEVG 327
           TVVD +TG SKDSRVRTSSGTFL RG D+ +  IEKRI+DFTFIPVE+GEGLQVLHY+VG
Sbjct: 130 TVVDEKTGGSKDSRVRTSSGTFLRRGHDEVVEVIEKRISDFTFIPVENGEGLQVLHYQVG 189

Query: 328 QKYEPHFDYFLDDYNTKNGGQRIATVLMYLSEVEEGGETVFPAAKGNFSSVPWWNELSDC 387
           QKYEPH+DYFLD++NTKNGGQRIATVLMYLS+V++GGETVFPAA+GN S+VPWWNELS C
Sbjct: 190 QKYEPHYDYFLDEFNTKNGGQRIATVLMYLSDVDDGGETVFPAARGNISAVPWWNELSKC 249

Query: 388 GKKGLSVKPKRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYK 441
           GK+GLSV PK+ DALLFW+M+PDASLDPSSLHGGCPV+KGNKWS+TKW  V E+K
Sbjct: 250 GKEGLSVLPKKRDALLFWNMRPDASLDPSSLHGGCPVVKGNKWSSTKWFHVHEFK 290

[Close]
BLAST of Spg012550 vs. TAIR 10
Match: AT4G35810.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 391.3 bits (1004), Expect = 9.9e-109
Identity = 198/302 (65.56%), Postives = 232/302 (76.82%), Query Frame = 0 

Query: 143 KHRQSRFPSRKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGSPKVHDLSSIV-- 202
           K +Q R   RKS S+ T  FT++++  FVILIL+ LGI S+P  +  S    DL++IV  
Sbjct: 4   KPKQLRNKPRKSFSTQT--FTVVVLVLFVILILVGLGIFSLPSTNKTSSMPMDLTTIVQT 63

Query: 203 ---RKTSDDVDEEKGEQWVEVISWEPRAFVYHNFLVSFLPIYDERFVFYTKEECEYLISL 262
              R++  D ++  G++W+EVISWEPRAFVYHNFL              T EECE+LISL
Sbjct: 64  IQERESFGDEEDGNGDRWLEVISWEPRAFVYHNFL--------------TNEECEHLISL 123

Query: 263 AKPHMQKSTVVDSETGKSKDSRVRTSSGTFLPRGRDKTIRTIEKRIADFTFIPVEHGEGL 322
           AKP M KS VVD +TGKS DSRVRTSSGTFL RG D+ +  IE RI+DFTFIP E+GEGL
Sbjct: 124 AKPSMMKSKVVDVKTGKSIDSRVRTSSGTFLNRGHDEIVEEIENRISDFTFIPPENGEGL 183

Query: 323 QVLHYEVGQKYEPHFDYFLDDYNTKNGGQRIATVLMYLSEVEEGGETVFPAAKGNFSSVP 382
           QVLHYEVGQ+YEPH DYF D++N + GGQRIATVLMYLS+V+EGGETVFPAAKGN S VP
Sbjct: 184 QVLHYEVGQRYEPHHDYFFDEFNVRKGGQRIATVLMYLSDVDEGGETVFPAAKGNVSDVP 243

Query: 383 WWNELSDCGKKGLSVKPKRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVE 440
           WW+ELS CGK+GLSV PK+ DALLFWSMKPDASLDPSSLHGGCPVIKGNKWS+TKW  V 
Sbjct: 244 WWDELSQCGKEGLSVLPKKRDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSSTKWFHVH 289

[Close]
BLAST of Spg012550 vs. TAIR 10
Match: AT5G66060.2 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 358.2 bits (918), Expect = 9.3e-99
Identity = 183/251 (72.91%), Postives = 206/251 (82.07%), Query Frame = 0 

Query: 141 MAKHRQSRFPSRKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGSPKVHDLSSIV 200
           MA+ R  R PS + SS STLVF +LIM TFVILILLA GILS+P N+ GS K +DL+SIV
Sbjct: 2   MARPRNHR-PSARKSSHSTLVFAVLIMSTFVILILLAFGILSVPSNNAGSSKANDLTSIV 61

Query: 201 RKT--SDDVDEEKGEQWVEVISWEPRAFVYHNFLVSFLPIYDERFVFYTKEECEYLISLA 260
           RKT      D+ K E+WVE+ISWEPRA VYHNFL                EEC+YLI LA
Sbjct: 62  RKTLQRSGEDDSKNERWVEIISWEPRASVYHNFL----------------EECKYLIELA 121

Query: 261 KPHMQKSTVVDSETGKSKDSRVRTSSGTFLPRGRDKTIRTIEKRIADFTFIPVEHGEGLQ 320
           KPHM+KSTVVD +TGKS DSRVRTSSGTFL RGRDKTIR IEKRI+DFTFIPVEHGEGLQ
Sbjct: 122 KPHMEKSTVVDEKTGKSTDSRVRTSSGTFLARGRDKTIREIEKRISDFTFIPVEHGEGLQ 181

Query: 321 VLHYEVGQKYEPHFDYFLDDYNTKNGGQRIATVLMYLSEVEEGGETVFPAAKGNFSSVPW 380
           VLHYE+GQKYEPH+DYF+D+YNT+NGGQRIATVLMYLS+VEEGGETVFPAAKGN+S+VPW
Sbjct: 182 VLHYEIGQKYEPHYDYFMDEYNTRNGGQRIATVLMYLSDVEEGGETVFPAAKGNYSAVPW 235

Query: 381 WNELSDCGKKG 390
           WNELS+CGK G
Sbjct: 242 WNELSECGKGG 235

The following BLAST results are available for this feature:
BLAST of Spg012550 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_038881910.12.0e-15693.33probable prolyl 4-hydroxylase 10 isoform X2 [Benincasa hispida][more]
XP_008440878.12.7e-15692.69PREDICTED: probable prolyl 4-hydroxylase 10 [Cucumis melo][more]
XP_004134841.14.6e-15692.36probable prolyl 4-hydroxylase 10 [Cucumis sativus] >XP_031742781.1 probable prol... [more]
XP_038881909.13.3e-15491.78probable prolyl 4-hydroxylase 10 isoform X1 [Benincasa hispida][more]
XP_022978860.17.3e-15491.03probable prolyl 4-hydroxylase 10 [Cucurbita maxima][more]
BLAST of Spg012550 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
F4JZ241.4e-12875.83Probable prolyl 4-hydroxylase 10 OS=Arabidopsis thaliana OX=3702 GN=P4H10 PE=3 S... [more]
Q9LN201.3e-11669.87Probable prolyl 4-hydroxylase 3 OS=Arabidopsis thaliana OX=3702 GN=P4H3 PE=2 SV=... [more]
Q24JN53.7e-11670.51Prolyl 4-hydroxylase 5 OS=Arabidopsis thaliana OX=3702 GN=P4H5 PE=2 SV=1[more]
F4JNU81.4e-10765.56Probable prolyl 4-hydroxylase 8 OS=Arabidopsis thaliana OX=3702 GN=P4H8 PE=3 SV=... [more]
Q8LAN34.0e-6249.80Probable prolyl 4-hydroxylase 4 OS=Arabidopsis thaliana OX=3702 GN=P4H4 PE=2 SV=... [more]
BLAST of Spg012550 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A1S3B1P61.3e-15692.69probable prolyl 4-hydroxylase 10 OS=Cucumis melo OX=3656 GN=LOC103485167 PE=4 SV... [more]
A0A0A0KJH92.2e-15692.36Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G... [more]
A0A6J1IRF13.5e-15491.03probable prolyl 4-hydroxylase 10 OS=Cucurbita maxima OX=3661 GN=LOC111478689 PE=... [more]
A0A6J1GDV61.0e-15391.03probable prolyl 4-hydroxylase 10 OS=Cucurbita moschata OX=3662 GN=LOC111453285 P... [more]
A0A6J1BWA61.5e-15290.03probable prolyl 4-hydroxylase 10 OS=Momordica charantia OX=3673 GN=LOC111005915 ... [more]
BLAST of Spg012550 vs. TAIR 10
Match NameE-valueIdentityDescription
AT5G66060.11.0e-12975.832-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT1G20270.19.0e-11869.872-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT2G17720.12.6e-11770.512-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT4G35810.19.9e-10965.562-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT5G66060.29.3e-9972.912-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006620Prolyl 4-hydroxylase, alpha subunitSMARTSM00702p4hccoord: 237..435
e-value: 5.6E-59
score: 211.9
IPR044862Prolyl 4-hydroxylase alpha subunit, Fe(2+) 2OG dioxygenase domainPFAMPF136402OG-FeII_Oxy_3coord: 317..435
e-value: 1.4E-21
score: 77.2
NoneNo IPR availableGENE3D2.60.120.620q2cbj1_9rhob like domaincoord: 215..436
e-value: 3.6E-77
score: 260.7
NoneNo IPR availablePANTHERPTHR10869:SF157PROLYL 4-HYDROXYLASE 10-RELATEDcoord: 141..440
IPR045054Prolyl 4-hydroxylasePANTHERPTHR10869PROLYL 4-HYDROXYLASE ALPHA SUBUNITcoord: 141..440
IPR005123Oxoglutarate/iron-dependent dioxygenasePROSITEPS51471FE2OG_OXYcoord: 313..436
score: 12.68751

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg012550.1Spg012550.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0018401 peptidyl-proline hydroxylation to 4-hydroxy-L-proline
cellular_component GO:0005783 endoplasmic reticulum
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005506 iron ion binding
molecular_function GO:0031418 L-ascorbic acid binding
molecular_function GO:0004656 procollagen-proline 4-dioxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen