Spg012481 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg012481 vs. NCBI nr
Match: XP_038881205.1 (protein NRT1/ PTR FAMILY 2.8-like isoform X1 [Benincasa hispida] >XP_038881206.1 protein NRT1/ PTR FAMILY 2.8-like isoform X1 [Benincasa hispida])

HSP 1 Score: 969.1 bits (2504), Expect = 1.7e-278
Identity = 490/573 (85.51%), Postives = 523/573 (91.27%), Query Frame = 0

Query: 54  HSSSDPPPSKSAHGGWRAVRFILANETFEKLASMSLIANLVLYLHTMYNLDNVDSAYVFQ 113
           HSSSD P  K+A GGWRAVRFIL NETFEKLASMSLI NLVLYLHTMYNLDNV SAYVFQ
Sbjct: 7   HSSSDAPRKKTARGGWRAVRFILGNETFEKLASMSLIGNLVLYLHTMYNLDNVASAYVFQ 66

Query: 114 IWGGTTNFAPLAGAFLADAYLGRFYTLLFGSIASFLGMGVLTLGAGLPQMRPPPCTTGES 173
           IWGGTTNFAPLAGAFLADAYLGRFYTLLFGSIASFLGMGVL+LGAGLPQMRPPPC +GES
Sbjct: 67  IWGGTTNFAPLAGAFLADAYLGRFYTLLFGSIASFLGMGVLSLGAGLPQMRPPPCISGES 126

Query: 174 NCPQPHPWQLAFLYLGLGLIVIGAGGIRPCNISFGADQFDTNTEKGRAKLESFLNWWYFL 233
           +CPQP PWQL FLYLGLGLIV+GAGGIRPCNISFGADQFDTNTEKGRAKLESFLNWWY L
Sbjct: 127 SCPQPQPWQLGFLYLGLGLIVVGAGGIRPCNISFGADQFDTNTEKGRAKLESFLNWWYLL 186

Query: 234 FSIALIIALTLVVYVQTNISWTLGFAIPTAAFVFSVLVFLSGRRFYICKAPQGSVFADMA 293
           FSIAL+IALT+VVYVQTNISWTLGFAIPTA FV S+L+FLSGRRFY+CKAPQGSVF+D+A
Sbjct: 187 FSIALVIALTIVVYVQTNISWTLGFAIPTACFVVSILIFLSGRRFYVCKAPQGSVFSDLA 246

Query: 294 KVIVATCHKRGIPKPSP-DDLHNPPMDSDSSKLAHTDRFLVFDKAATVVDSATELDEEGR 353
           KV+VA   KR IPKP+  D LHNPPM  +S KLAHTDRFLVFDKAATVVDS+ ELDEEG+
Sbjct: 247 KVVVAMFRKRKIPKPNQIDHLHNPPM--NSCKLAHTDRFLVFDKAATVVDSSIELDEEGK 306

Query: 354 SKNEWRLCSVHQVEQFKCVVGIVPVWAAGIACFISMQQMSSFGILQAIQMNRFLGPHFQI 413
           SK+EWRLCSVHQVEQFKCVVGI+PVW AGIACFISMQQM SFGILQAIQMNRF+GPHFQI
Sbjct: 307 SKDEWRLCSVHQVEQFKCVVGIIPVWLAGIACFISMQQMGSFGILQAIQMNRFIGPHFQI 366

Query: 414 PPAWMSLTPMIALSIWIYIYEKYVEFMKRKTHHNKRLSMKRRIDIGIVMSVLCMVVAGIL 473
           PPAWMSLTPMIALSIWIYIYEKYVEFMK+KTH NKR SMK RI+IGIVMSVLCMVVAG+L
Sbjct: 367 PPAWMSLTPMIALSIWIYIYEKYVEFMKKKTHSNKRFSMKTRIEIGIVMSVLCMVVAGLL 426

Query: 474 EKVRRDSALENKSFVSPLHVSVLIPEFVLSGLTEAFAAIAVMELLTTHLPESLRTVAGAI 533
           EK+RRD+A+ENKSFVSPLHV +LIPEF LSGLTEAFAAIAVMELLTTHLPESLRTVAGAI
Sbjct: 427 EKLRRDAAVENKSFVSPLHVWMLIPEFALSGLTEAFAAIAVMELLTTHLPESLRTVAGAI 486

Query: 534 FFLSLSLASYLSSVIVGILSKISK----KTPWLGGNDLNKNRLDYFFYVVAVIAGLNFFY 593
           FFLSLSLASYLSSV++GI+SKI +    +  WLGGNDLNKNRLDYFFYVV  IA LNFFY
Sbjct: 487 FFLSLSLASYLSSVLMGIVSKIGRMEAGEMQWLGGNDLNKNRLDYFFYVVGGIAALNFFY 546

Query: 594 FRLVAAKFLPKGDVDPRQKAGERQLEDEESGYR 622
           FR  AA FLP  DVDPRQKA +RQLED E G R
Sbjct: 547 FRFFAAGFLPSPDVDPRQKAHQRQLEDAELGRR 577

BLAST of Spg012481 vs. NCBI nr
Match: XP_023518809.1 (protein NRT1/ PTR FAMILY 2.8-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 964.1 bits (2491), Expect = 5.6e-277
Identity = 482/579 (83.25%), Postives = 532/579 (91.88%), Query Frame = 0

Query: 47  MENGSLDHSSSDPPPSKSAHGGWRAVRFILANETFEKLASMSLIANLVLYLHTMYNLDNV 106
           M+NG+L  SS DP P K   GGWRAVRFILANETFEKLASMSLIANL+LYLHTMYNLDNV
Sbjct: 1   MDNGTL-RSSLDPSP-KPPLGGWRAVRFILANETFEKLASMSLIANLILYLHTMYNLDNV 60

Query: 107 DSAYVFQIWGGTTNFAPLAGAFLADAYLGRFYTLLFGSIASFLGMGVLTLGAGLPQMRPP 166
           DSAYVFQIWGGTTNF+PLAGAFLADAYLGRFYTLLFGSIASFLGMGVLTLGA LP+MRPP
Sbjct: 61  DSAYVFQIWGGTTNFSPLAGAFLADAYLGRFYTLLFGSIASFLGMGVLTLGAALPEMRPP 120

Query: 167 PCTTGESNCPQPHPWQLAFLYLGLGLIVIGAGGIRPCNISFGADQFDTNTEKGRAKLESF 226
           PC++G+++C Q   WQLAFLYLGLGL+VIGAGGIRPCNISFGADQFDTNTEKGRAKLESF
Sbjct: 121 PCSSGKTDCIQSKSWQLAFLYLGLGLLVIGAGGIRPCNISFGADQFDTNTEKGRAKLESF 180

Query: 227 LNWWYFLFSIALIIALTLVVYVQTNISWTLGFAIPTAAFVFSVLVFLSGRRFYICKAPQG 286
           LNWWYFLFSIAL+IALTLVVY+QTNISWTLGFAIPTAAF+FS+++FL GR  YIC+ PQG
Sbjct: 181 LNWWYFLFSIALVIALTLVVYIQTNISWTLGFAIPTAAFIFSIVIFLLGRHLYICRIPQG 240

Query: 287 SVFADMAKVIVATCHKRGIPKPSPDDLHNPPMDSDSSKLAHTDRFLVFDKAATVVDSATE 346
           SVFADMAKV++AT  KR IP+PSP+ LH+PPM  DSSKL HT+RFLVFDKAA V+DS+ E
Sbjct: 241 SVFADMAKVLIATYRKRQIPRPSPNHLHDPPM--DSSKLPHTNRFLVFDKAAMVIDSSME 300

Query: 347 LDEEGRSKNEWRLCSVHQVEQFKCVVGIVPVWAAGIACFISMQQMSSFGILQAIQMNRFL 406
           LDE+GRSKNEW+LC+VHQVEQ KCVVGIVP+WAAGI+CFISMQQM SFGILQAIQMNRFL
Sbjct: 301 LDEQGRSKNEWKLCTVHQVEQLKCVVGIVPIWAAGISCFISMQQMGSFGILQAIQMNRFL 360

Query: 407 GPHFQIPPAWMSLTPMIALSIWIYIYEKYVEFMKRKTHHNKRLSMKRRIDIGIVMSVLCM 466
           GPHF+IPPAWMSL PMIALS+WIYIYEKYVEFMKRKTHHNKR SMK RI+IGIVMSVLCM
Sbjct: 361 GPHFEIPPAWMSLIPMIALSLWIYIYEKYVEFMKRKTHHNKRFSMKMRIEIGIVMSVLCM 420

Query: 467 VVAGILEKVRRDSALENKSFVSPLHVSVLIPEFVLSGLTEAFAAIAVMELLTTHLPESLR 526
           VVAGILEK+RRDSA+EN++FVSPLHVSVLIPEF LSGLTEAFAAI+VMELLTT LPESLR
Sbjct: 421 VVAGILEKIRRDSAIENRTFVSPLHVSVLIPEFALSGLTEAFAAISVMELLTTQLPESLR 480

Query: 527 TVAGAIFFLSLSLASYLSSVIVGILSKISK----KTPWLGGNDLNKNRLDYFFYVVAVIA 586
           TVAGAIFFLSLSLASYLSSV++GI++++SK    KT WL GNDLNKN LDYFFYVV VIA
Sbjct: 481 TVAGAIFFLSLSLASYLSSVLMGIVNRVSKTGDSKTSWLEGNDLNKNHLDYFFYVVGVIA 540

Query: 587 GLNFFYFRLVAAKFLPKGDVDPRQKAGERQLEDEESGYR 622
            LNFFYFR V  +FLP+GDVDPR KAGER+ +DEESG+R
Sbjct: 541 ALNFFYFRFVGTRFLPEGDVDPRGKAGERRSDDEESGFR 575

BLAST of Spg012481 vs. NCBI nr
Match: KAG6595347.1 (Protein NRT1/ PTR FAMILY 2.8, partial [Cucurbita argyrosperma subsp. sororia] >KAG7027355.1 Protein NRT1/ PTR FAMILY 2.8, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 961.4 bits (2484), Expect = 3.6e-276
Identity = 480/579 (82.90%), Postives = 531/579 (91.71%), Query Frame = 0

Query: 47  MENGSLDHSSSDPPPSKSAHGGWRAVRFILANETFEKLASMSLIANLVLYLHTMYNLDNV 106
           M+NG+L  SS DP P K   GGWRAVRFILANETFEKLASMSLIANL+LYLHTMYNLDNV
Sbjct: 1   MDNGTL-RSSLDPSP-KPPLGGWRAVRFILANETFEKLASMSLIANLILYLHTMYNLDNV 60

Query: 107 DSAYVFQIWGGTTNFAPLAGAFLADAYLGRFYTLLFGSIASFLGMGVLTLGAGLPQMRPP 166
           DSAYVFQIWGGTTNF+PLAGAFLADAYLGRFYTLLFGSIASFLGMGVLTLGA LP+MRPP
Sbjct: 61  DSAYVFQIWGGTTNFSPLAGAFLADAYLGRFYTLLFGSIASFLGMGVLTLGAALPEMRPP 120

Query: 167 PCTTGESNCPQPHPWQLAFLYLGLGLIVIGAGGIRPCNISFGADQFDTNTEKGRAKLESF 226
           PC++G+++C Q   WQLAFLYLGLGL+VIGAGGIRPCNISFGADQFDTNTEKGRAKLESF
Sbjct: 121 PCSSGKTDCIQSKSWQLAFLYLGLGLLVIGAGGIRPCNISFGADQFDTNTEKGRAKLESF 180

Query: 227 LNWWYFLFSIALIIALTLVVYVQTNISWTLGFAIPTAAFVFSVLVFLSGRRFYICKAPQG 286
           LNWWYFLFSIAL+IALTLVVY+QTNISWTLGFAIPTAAF+FS+++FL GR  YIC+ PQG
Sbjct: 181 LNWWYFLFSIALVIALTLVVYIQTNISWTLGFAIPTAAFIFSIVIFLLGRHLYICRIPQG 240

Query: 287 SVFADMAKVIVATCHKRGIPKPSPDDLHNPPMDSDSSKLAHTDRFLVFDKAATVVDSATE 346
           SVFADMAKV++AT  KR IP+PSP+ LH+PPM  DSSKL HT+RFLVFDKAA V+DS+ E
Sbjct: 241 SVFADMAKVLIATYRKRQIPRPSPNHLHDPPM--DSSKLPHTNRFLVFDKAAMVIDSSME 300

Query: 347 LDEEGRSKNEWRLCSVHQVEQFKCVVGIVPVWAAGIACFISMQQMSSFGILQAIQMNRFL 406
           LDE+GRSKNEW+LC+VHQVEQ KCVVGIVP+WAAGI+CFISMQQM SFGILQAIQMNRFL
Sbjct: 301 LDEQGRSKNEWKLCTVHQVEQLKCVVGIVPIWAAGISCFISMQQMGSFGILQAIQMNRFL 360

Query: 407 GPHFQIPPAWMSLTPMIALSIWIYIYEKYVEFMKRKTHHNKRLSMKRRIDIGIVMSVLCM 466
           GPHF+IPPAWMSL PMIALS+WIYIYEKYVEFM+RKTHHNKR SMK RI+IGIVMSVLCM
Sbjct: 361 GPHFEIPPAWMSLIPMIALSLWIYIYEKYVEFMRRKTHHNKRFSMKMRIEIGIVMSVLCM 420

Query: 467 VVAGILEKVRRDSALENKSFVSPLHVSVLIPEFVLSGLTEAFAAIAVMELLTTHLPESLR 526
           +VAGILEK+RRDSA+EN++FVSPLHVSVLIPEF LSGLTEAFAAI+VMELLTT LPESLR
Sbjct: 421 IVAGILEKIRRDSAIENRTFVSPLHVSVLIPEFALSGLTEAFAAISVMELLTTQLPESLR 480

Query: 527 TVAGAIFFLSLSLASYLSSVIVGILSKISK----KTPWLGGNDLNKNRLDYFFYVVAVIA 586
           TVAGAIFFLSLSLASYLSSV++GI++++SK    KT WL GNDLNKN LDYFFYVV VIA
Sbjct: 481 TVAGAIFFLSLSLASYLSSVLMGIVNRVSKTGDSKTSWLEGNDLNKNHLDYFFYVVGVIA 540

Query: 587 GLNFFYFRLVAAKFLPKGDVDPRQKAGERQLEDEESGYR 622
            LNFFYFR V  +FLP+GDVDPR KA ER+ EDEESG+R
Sbjct: 541 ALNFFYFRFVGTRFLPEGDVDPRVKASERRSEDEESGFR 575

BLAST of Spg012481 vs. NCBI nr
Match: XP_022931895.1 (protein NRT1/ PTR FAMILY 2.8-like [Cucurbita moschata])

HSP 1 Score: 961.4 bits (2484), Expect = 3.6e-276
Identity = 480/579 (82.90%), Postives = 531/579 (91.71%), Query Frame = 0

Query: 47  MENGSLDHSSSDPPPSKSAHGGWRAVRFILANETFEKLASMSLIANLVLYLHTMYNLDNV 106
           M+NG+L  SS DP P K   GGWRAVRFILANETFEKLASMSLIANL+LYLHTMYNLDNV
Sbjct: 1   MDNGTL-RSSLDPSP-KPQLGGWRAVRFILANETFEKLASMSLIANLILYLHTMYNLDNV 60

Query: 107 DSAYVFQIWGGTTNFAPLAGAFLADAYLGRFYTLLFGSIASFLGMGVLTLGAGLPQMRPP 166
           DSAYVFQIWGGTTNF+PLAGAFLADAYLGRFYTLLFGSIASFLGMGVLTLGA LP+MRPP
Sbjct: 61  DSAYVFQIWGGTTNFSPLAGAFLADAYLGRFYTLLFGSIASFLGMGVLTLGAALPEMRPP 120

Query: 167 PCTTGESNCPQPHPWQLAFLYLGLGLIVIGAGGIRPCNISFGADQFDTNTEKGRAKLESF 226
           PC++G+++C Q   WQLAFLYLGLGL+VIGAGGIRPCNISFGADQFDTNTEKGRAKLESF
Sbjct: 121 PCSSGKTDCIQSKSWQLAFLYLGLGLLVIGAGGIRPCNISFGADQFDTNTEKGRAKLESF 180

Query: 227 LNWWYFLFSIALIIALTLVVYVQTNISWTLGFAIPTAAFVFSVLVFLSGRRFYICKAPQG 286
           LNWWYFLFSIAL+IALTLVVY+QTNISWTLGFAIPTAAF+FS+++FL GR  YIC+ PQG
Sbjct: 181 LNWWYFLFSIALVIALTLVVYIQTNISWTLGFAIPTAAFIFSIVIFLLGRHLYICRIPQG 240

Query: 287 SVFADMAKVIVATCHKRGIPKPSPDDLHNPPMDSDSSKLAHTDRFLVFDKAATVVDSATE 346
           SVFADMAKV++AT  KR IP+PSP+ LH+PPM  DSSKL HT+RFLVFDKAA V+DS+ E
Sbjct: 241 SVFADMAKVLIATYRKRQIPRPSPNHLHDPPM--DSSKLPHTNRFLVFDKAAMVIDSSME 300

Query: 347 LDEEGRSKNEWRLCSVHQVEQFKCVVGIVPVWAAGIACFISMQQMSSFGILQAIQMNRFL 406
           LDE+GRSKNEW+LC+VHQVEQ KCVVGIVP+WAAGI+CFISMQQM SFGILQAIQMNRFL
Sbjct: 301 LDEQGRSKNEWKLCTVHQVEQLKCVVGIVPIWAAGISCFISMQQMGSFGILQAIQMNRFL 360

Query: 407 GPHFQIPPAWMSLTPMIALSIWIYIYEKYVEFMKRKTHHNKRLSMKRRIDIGIVMSVLCM 466
           GPHF+IPPAWMSL PMIALS+WIYIYEKYVEFM+RKTHHNKR SMK RI+IGIVMSVLCM
Sbjct: 361 GPHFEIPPAWMSLIPMIALSLWIYIYEKYVEFMRRKTHHNKRFSMKMRIEIGIVMSVLCM 420

Query: 467 VVAGILEKVRRDSALENKSFVSPLHVSVLIPEFVLSGLTEAFAAIAVMELLTTHLPESLR 526
           +VAGILEK+RRDSA+EN++FVSPLHVSVLIPEF LSGLTEAFAAI+VMELLTT LPESLR
Sbjct: 421 IVAGILEKIRRDSAIENRTFVSPLHVSVLIPEFALSGLTEAFAAISVMELLTTQLPESLR 480

Query: 527 TVAGAIFFLSLSLASYLSSVIVGILSKISK----KTPWLGGNDLNKNRLDYFFYVVAVIA 586
           TVAGAIFFLSLSLASYLSSV++GI++++SK    KT WL GNDLNKN LDYFFYVV VIA
Sbjct: 481 TVAGAIFFLSLSLASYLSSVLMGIVNRVSKTGDSKTSWLEGNDLNKNHLDYFFYVVGVIA 540

Query: 587 GLNFFYFRLVAAKFLPKGDVDPRQKAGERQLEDEESGYR 622
            LNFFYFR V  +FLP+GDVDPR KA ER+ EDEESG+R
Sbjct: 541 ALNFFYFRFVGTRFLPEGDVDPRVKASERRSEDEESGFR 575

BLAST of Spg012481 vs. NCBI nr
Match: XP_004142186.1 (protein NRT1/ PTR FAMILY 2.8 [Cucumis sativus] >KAE8649550.1 hypothetical protein Csa_017951 [Cucumis sativus])

HSP 1 Score: 944.9 bits (2441), Expect = 3.5e-271
Identity = 479/576 (83.16%), Postives = 511/576 (88.72%), Query Frame = 0

Query: 47  MENGSLDHSSSDPPPSKSAHGGWRAVRFILANETFEKLASMSLIANLVLYLHTMYNLDNV 106
           MENG+L H+S D   +K   GGWRAVRFIL NETFEKLASMSLI NLVLYLHTMYNLDNV
Sbjct: 1   MENGNLLHASLDASRNKETRGGWRAVRFILGNETFEKLASMSLIGNLVLYLHTMYNLDNV 60

Query: 107 DSAYVFQIWGGTTNFAPLAGAFLADAYLGRFYTLLFGSIASFLGMGVLTLGAGLPQMRPP 166
            SAYVFQIWGGTTNFAPLAGAFLADAYLGRFYTLLFGS+AS LGMGVLTLGAGLPQMRPP
Sbjct: 61  ASAYVFQIWGGTTNFAPLAGAFLADAYLGRFYTLLFGSVASLLGMGVLTLGAGLPQMRPP 120

Query: 167 PCTTGESNCPQPHPWQLAFLYLGLGLIVIGAGGIRPCNISFGADQFDTNTEKGRAKLESF 226
           PC +GESNCPQP PWQL FLYLGLGLIVIGAGGIRPCNISFGADQFDTNTEKGRAKLESF
Sbjct: 121 PCISGESNCPQPLPWQLGFLYLGLGLIVIGAGGIRPCNISFGADQFDTNTEKGRAKLESF 180

Query: 227 LNWWYFLFSIALIIALTLVVYVQTNISWTLGFAIPTAAFVFSVLVFLSGRRFYICKAPQG 286
           LNWWYFLFSI+L+IALT+VVYVQTNISWTLGF IPTA FVFS+ +FL G R+YICKAPQG
Sbjct: 181 LNWWYFLFSISLVIALTIVVYVQTNISWTLGFGIPTACFVFSIFIFLLGHRYYICKAPQG 240

Query: 287 SVFADMAKVIVATCHKRGIPKPSP-DDLHNPPMDSDSSKLAHTDRFLVFDKAATVVDSAT 346
           SVFADMAKV+VATC K  IPK +  D LHNPPM  +SSKLAHT+RFL+FDKAATVVDS+ 
Sbjct: 241 SVFADMAKVVVATCRKWQIPKTTQFDQLHNPPM--NSSKLAHTNRFLIFDKAATVVDSSV 300

Query: 347 ELDEEGRSKNEWRLCSVHQVEQFKCVVGIVPVWAAGIACFISMQQMSSFGILQAIQMNRF 406
           ELDEEG+SKNEWRLCSVHQVEQFKCVVGI PVW AGI CF+SMQQMSSFGILQAIQMNR 
Sbjct: 301 ELDEEGKSKNEWRLCSVHQVEQFKCVVGIFPVWLAGIPCFMSMQQMSSFGILQAIQMNRS 360

Query: 407 LGPHFQIPPAWMSLTPMIALSIWIYIYEKYVEFMKRKTHHNKRLSMKRRIDIGIVMSVLC 466
           +GPHFQIPPAWMSLTPMIALSIWIYIYEKYVE MK+KT  NKR SMK RI+IGIVMSVLC
Sbjct: 361 IGPHFQIPPAWMSLTPMIALSIWIYIYEKYVEHMKKKTQSNKRFSMKTRIEIGIVMSVLC 420

Query: 467 MVVAGILEKVRRDSALENKSFVSPLHVSVLIPEFVLSGLTEAFAAIAVMELLTTHLPESL 526
           MVVAG+LEK RRD+A+ENKSF+SPLHV VLIPEF LSGLTEAF AIAVMELLTTHLPESL
Sbjct: 421 MVVAGVLEKFRRDAAVENKSFISPLHVWVLIPEFALSGLTEAFGAIAVMELLTTHLPESL 480

Query: 527 RTVAGAIFFLSLSLASYLSSVIVGILSKISKKTPWLGGNDLNKNRLDYFFYVVAVIAGLN 586
           RTVAGAIFF SLSLASYLSSV+ GI+  + ++  WLGGNDLNKNRLDYFF VV VIA LN
Sbjct: 481 RTVAGAIFFFSLSLASYLSSVLTGIVRGVERE--WLGGNDLNKNRLDYFFSVVGVIAALN 540

Query: 587 FFYFRLVAAKFLPKGDVDPRQKAGERQLEDEESGYR 622
           FFYFR  A  FLPK DVDP  KA + QLED E G R
Sbjct: 541 FFYFRFFAVAFLPKPDVDPSHKAHQMQLEDAELGRR 572

BLAST of Spg012481 vs. ExPASy Swiss-Prot
Match: Q3E8X3 (Protein NRT1/ PTR FAMILY 2.8 OS=Arabidopsis thaliana OX=3702 GN=NPF2.8 PE=2 SV=2)

HSP 1 Score: 567.0 bits (1460), Expect = 2.6e-160
Identity = 291/554 (52.53%), Postives = 385/554 (69.49%), Query Frame = 0

Query: 52  LDHSSSDPPPS---KSAHGGWRAVRFILANETFEKLASMSLIANLVLYLHTMYNLDNVDS 111
           +D  SS P      K   GGWRA+++I+ANE+FEKLASMSLI NL +YL T YNL  V  
Sbjct: 1   MDVESSSPSSHALIKKEKGGWRAIKYIIANESFEKLASMSLIGNLSVYLMTKYNLGGVFL 60

Query: 112 AYVFQIWGGTTNFAPLAGAFLADAYLGRFYTLLFGSIASFLGMGVLTLGAGLPQMRPPPC 171
             V  IW G+ N   LAGAF++DAYLGRF+TLL GSIASF+GMG+  L A LP +RP  C
Sbjct: 61  VNVINIWFGSCNILTLAGAFVSDAYLGRFWTLLLGSIASFIGMGIFALTAALPSLRPDAC 120

Query: 172 TTGESNCPQPHPWQLAFLYLGLGLIVIGAGGIRPCNISFGADQFDTNTEKGRAKLESFLN 231
               +   QP  WQL  L+ GLGL+ IGAGG+RPCNI+FGADQFDT+T+KG+A LE+F N
Sbjct: 121 IDPSNCSNQPAKWQLGVLFSGLGLLAIGAGGVRPCNIAFGADQFDTSTKKGKAHLETFFN 180

Query: 232 WWYFLFSIALIIALTLVVYVQTNISWTLGFAIPTAAFVFSVLVFLSGRRFYICKAPQGSV 291
           WWYF F++AL+IALT VVY+QTNISW +GF IPTA    S+  F+ G+  YIC   +GSV
Sbjct: 181 WWYFSFTVALVIALTGVVYIQTNISWVIGFVIPTACLALSITTFVIGQHTYICAKAEGSV 240

Query: 292 FADMAKVIVATCHKRGIPKPSPDDLH--NPPMDSDSSKLAHTDRFLVFDKAATVVDSATE 351
           FAD+ KV+ A C KR + KP  D      P  D   + L      L F   A++V +  E
Sbjct: 241 FADIVKVVTAACKKRKV-KPGSDITFYIGPSNDGSPTTLVRDKHRLRFFDKASIVTNPNE 300

Query: 352 LDEEGRSKNEWRLCSVHQVEQFKCVVGIVPVWAAGIACFISMQQMSSFGILQAIQMNRFL 411
           L+E+G +K +WRLCSV QV+  KCV  I+PVW  GIACFI   Q + +GILQA+QM++  
Sbjct: 301 LNEDGNAKYKWRLCSVQQVKNLKCVTAILPVWVTGIACFILTDQQNIYGILQAMQMDKTF 360

Query: 412 GPH-FQIPPAWMSLTPMIALSIWIYIYE-KYVEFMKRKTHHNKRLSMKRRIDIGIVMSVL 471
           GPH FQ+P  WM+L  MI L+IWI +YE   +  +K+ T   KRL++K RI+  IVM ++
Sbjct: 361 GPHNFQVPAGWMNLVSMITLAIWISLYECVIIPIVKQITGRKKRLTLKHRIE--IVMGII 420

Query: 472 CMVVAGILEKVRRDSALENKSFVSPLHVSVLIPEFVLSGLTEAFAAIAVMELLTTHLPES 531
           CM+VAG  EK RR SAL+N SFVSP+ + +L+P+F L+GLTEAF+A+A+ME LT  +PE 
Sbjct: 421 CMIVAGFQEKKRRASALKNGSFVSPVSIVMLLPQFALAGLTEAFSAVALMEFLTVRMPEH 480

Query: 532 LRTVAGAIFFLSLSLASYLSSVIVGILSKISKK--TPWLGGNDLNKNRLDYFFYVVAVIA 591
           +R VAGAIFFLS S+ASY+ ++++ ++  +++K    WLG  DLNKNRL+ +F+++A I 
Sbjct: 481 MRAVAGAIFFLSSSIASYICTLLINVIDAVTRKEGKSWLGDKDLNKNRLENYFFIIAGIQ 540

Query: 592 GLNFFYFRLVAAKF 597
             N  YFRL A+++
Sbjct: 541 VANLLYFRLFASRY 551

BLAST of Spg012481 vs. ExPASy Swiss-Prot
Match: Q8RX77 (Protein NRT1/ PTR FAMILY 2.13 OS=Arabidopsis thaliana OX=3702 GN=NPF2.13 PE=1 SV=1)

HSP 1 Score: 424.9 bits (1091), Expect = 1.6e-117
Identity = 227/569 (39.89%), Postives = 360/569 (63.27%), Query Frame = 0

Query: 67  GGWRAVRFILANETFEKLASMSLIANLVLYLHTMYNLDNVDSAYVFQIWGGTTNFAPLAG 126
           GGWRAV FIL NET E+L S+ L+AN ++YL  +++L+ VD+A V  IW G TN  PL G
Sbjct: 53  GGWRAVSFILGNETLERLGSIGLLANFMVYLTKVFHLEQVDAANVINIWSGFTNLTPLVG 112

Query: 127 AFLADAYLGRFYTLLFGSIASFLGMGVLTLGAGLPQMRPPPCTTGES-NCPQPHPWQLAF 186
           A+++D Y+GRF T+ F S A+ LG+  +TL A  PQ+ P  C + +  +C  P+  Q+  
Sbjct: 113 AYISDTYVGRFKTIAFASFATLLGLITITLTASFPQLHPASCNSQDPLSCGGPNKLQIGV 172

Query: 187 LYLGLGLIVIGAGGIRPCNISFGADQFDTNTEKGRAKLESFLNWWYFLFSIALIIALTLV 246
           L LGL  + +G+GGIRPC+I FG DQFD  TE+G   + SF NW+Y  F++ LII  T+V
Sbjct: 173 LLLGLCFLSVGSGGIRPCSIPFGVDQFDQRTEEGVKGVASFFNWYYMTFTVVLIITQTVV 232

Query: 247 VYVQTNISWTLGFAIPTAAFVFSVLVFLSGRRFYICKAPQGSVFADMAKVIVATCHKRGI 306
           VY+Q  +SW +GF+IPT     +V++F +G + Y+   P+GS+F+ +A+VIVA   KR +
Sbjct: 233 VYIQDQVSWIIGFSIPTGLMALAVVMFFAGMKRYVYVKPEGSIFSGIAQVIVAARKKRKL 292

Query: 307 PKPSPDD----LHNPPMDSD-SSKLAHTDRFLVFDKAATVVDSATELDEEGRSKNEWRLC 366
             P+ DD     ++P + S   SKL  +++F   DKAA V++   +L  EG   ++WRLC
Sbjct: 293 KLPAEDDGTVTYYDPAIKSSVLSKLHRSNQFRCLDKAAVVIEG--DLTPEGPPADKWRLC 352

Query: 367 SVHQVEQFKCVVGIVPVWAAGIACFISMQQMSSFGILQAIQMNRFLGPHFQIPPAWMSLT 426
           SV +VE+ KC++ IVP+W+AGI    +M    +F + QA++M+R LGP F+IP   +S+ 
Sbjct: 353 SVQEVEEVKCLIRIVPIWSAGIISLAAMTTQGTFTVSQALKMDRNLGPKFEIPAGSLSVI 412

Query: 427 PMIALSIWIYIYEK-YVEFMKRKTHHNKRLSMKRRIDIGIVMSVLCMVVAGILEKVRRDS 486
            ++ + I++  Y++ +V FM+R T H   +++ +RI  GIV ++  M+VAGI+E++RR  
Sbjct: 413 SLLTIGIFLPFYDRVFVPFMRRITGHKSGITLLQRIGTGIVFAIFSMIVAGIVERMRRIR 472

Query: 487 ALE--NKSFVSPLHVSVLIPEFVLSGLTEAFAAIAVMELLTTHLPESLRTVAGAIFFLSL 546
           ++   + + ++P+ V  L P+ +L GL EAF  I  +E   +  PE +R++A ++F LS 
Sbjct: 473 SINAGDPTGMTPMSVFWLSPQLILMGLCEAFNIIGQIEFFNSQFPEHMRSIANSLFSLSF 532

Query: 547 SLASYLSSVIVGILSKIS---KKTPWLGGNDLNKNRLDYFFYVVAVIAGLNFFYFRLVAA 606
           + +SYLSS +V ++ K S    +  WL  N LN  +LDYF+Y++AV+  +N  YF   A 
Sbjct: 533 AGSSYLSSFLVTVVHKFSGGHDRPDWLNKN-LNAGKLDYFYYLIAVLGVVNLVYFWYCAR 592

Query: 607 KF-----LPKGDVDPRQKAGERQLEDEES 619
            +     LP  D +  + + + ++  ++S
Sbjct: 593 GYRYKVGLPIEDFEEDKSSDDVEMTSKKS 618

BLAST of Spg012481 vs. ExPASy Swiss-Prot
Match: Q9LFX9 (Protein NRT1/ PTR FAMILY 2.12 OS=Arabidopsis thaliana OX=3702 GN=NPF2.12 PE=1 SV=2)

HSP 1 Score: 423.7 bits (1088), Expect = 3.6e-117
Identity = 238/565 (42.12%), Postives = 353/565 (62.48%), Query Frame = 0

Query: 67  GGWRAVRFILANETFEKLASMSLIANLVLYLHTMYNLDNVDSAYVFQIWGGTTNFAPLAG 126
           GGWRA+ FIL NET EKL S+ + AN +LYL  +++++ V++  V+ +W G TNFAPL G
Sbjct: 16  GGWRAITFILGNETLEKLGSIGVSANFMLYLRNVFHMEPVEAFNVYYLWMGLTNFAPLLG 75

Query: 127 AFLADAYLGRFYTLLFGSIASFLGMGVLTLGAGLPQMRPPPCTTGE-SNCPQPHPWQLAF 186
           A ++DAY+GRF T+ + S+ S LG+  +TL A LPQ+ PPPC       C  P+  QL  
Sbjct: 76  ALISDAYIGRFKTIAYASLFSILGLMTVTLTACLPQLHPPPCNNPHPDECDDPNKLQLGI 135

Query: 187 LYLGLGLIVIGAGGIRPCNISFGADQFDTNTEKGRAKLESFLNWWYFLFSIALIIALTLV 246
           L+LGLG + IG+GGIRPC+I FG DQFD  TE+G   + SF NW+Y   ++ LI + T+V
Sbjct: 136 LFLGLGFLSIGSGGIRPCSIPFGVDQFDQRTEQGLKGVASFFNWYYLTLTMVLIFSHTVV 195

Query: 247 VYVQTNISWTLGFAIPTAAFVFSVLVFLSGRRFYICKAPQGSVFADMAKVIVATCHKRGI 306
           VY+QT +SW +GF+IPT+    +V++F  G RFY+   P+GSVF+ +A+VIVA   KR +
Sbjct: 196 VYLQT-VSWVIGFSIPTSLMACAVVLFFVGMRFYVYVKPEGSVFSGIARVIVAARKKRDL 255

Query: 307 PKPSPDD----LHNPPMDSD-SSKLAHTDRFLVFDKAATVVDSATELDEEGRSKNEWRLC 366
                DD     + PP+     SKL  TD+F   DKAA ++D   +L  EG   N+WRLC
Sbjct: 256 KISLVDDGTEEYYEPPVKPGVLSKLPLTDQFKFLDKAAVILDG--DLTSEGVPANKWRLC 315

Query: 367 SVHQVEQFKCVVGIVPVWAAGIACFISMQQMSSFGILQAIQMNRFLGPHFQIPPAWMSLT 426
           S+ +VE+ KC++ +VPVW+AGI   ++M   ++F + QA +M+R +GPHF+IP A +++ 
Sbjct: 316 SIQEVEEVKCLIRVVPVWSAGIISIVAMTTQATFMVFQATKMDRHMGPHFEIPAASITVI 375

Query: 427 PMIALSIWIYIYEK-YVEFMKRKTHHNKRLSMKRRIDIGIVMSVLCMVVAGILEKVRRDS 486
             I + IW+ IYE   V F+ R      R+++ +R+ IGIV ++L M  AG +E VRR  
Sbjct: 376 SYITIGIWVPIYEHLLVPFLWRM--RKFRVTLLQRMGIGIVFAILSMFTAGFVEGVRRTR 435

Query: 487 ALENKSFVSPLHVSVLIPEFVLSGLTEAFAAIAVMELLTTHLPESLRTVAGAIFFLSLSL 546
           A E    ++ + V  L    +L GL E+F  I ++E   +  PE +R++A ++F LS + 
Sbjct: 436 ATE----MTQMSVFWLALPLILMGLCESFNFIGLIEFFNSQFPEHMRSIANSLFPLSFAA 495

Query: 547 ASYLSSVIVGILSKIS--KKTP-WLGGNDLNKNRLDYFFYVVAVIAGLNFFYFRLVAAKF 606
           A+YLSS++V  + K+S  K  P WL   DL++ +LDYF+Y++AV+  +N  YF   A ++
Sbjct: 496 ANYLSSLLVTTVHKVSGTKDHPDWL-NKDLDRGKLDYFYYLIAVLGVVNLVYFWYCAHRY 555

Query: 607 LPKGDVDPRQKAGERQLEDEESGYR 622
             K          E+ L D E   R
Sbjct: 556 QYKAGSQIEDFNEEKSLLDIEPNQR 570

BLAST of Spg012481 vs. ExPASy Swiss-Prot
Match: Q9LV10 (Protein NRT1/ PTR FAMILY 2.11 OS=Arabidopsis thaliana OX=3702 GN=NPF2.11 PE=1 SV=1)

HSP 1 Score: 383.3 bits (983), Expect = 5.4e-105
Identity = 216/561 (38.50%), Postives = 341/561 (60.78%), Query Frame = 0

Query: 63  KSAHGGWRAVRFILANETFEKLASMSLIANLVLYLHTMYNLDNVDSAYVFQIWGGTTNFA 122
           K  + GW+ + FI+ NETFEKL  +  ++NL++YL  ++NL ++ +A +   + GT NF 
Sbjct: 41  KVVYRGWKVMPFIIGNETFEKLGIIGTLSNLLVYLTAVFNLKSITAATIINAFSGTINFG 100

Query: 123 PLAGAFLADAYLGRFYTLLFGSIASFLGMGVLTLGAGLPQMRPPPC-TTGESNCPQPHPW 182
               AFL D Y GR+ TL    IA FLG  V+ L A +PQ+ P  C T  +S C  P   
Sbjct: 101 TFVAAFLCDTYFGRYKTLSVAVIACFLGSFVILLTAAVPQLHPAACGTAADSICNGPSGG 160

Query: 183 QLAFLYLGLGLIVIGAGGIRPCNISFGADQFDTNTEKGRAKLESFLNWWYFLFSIALIIA 242
           Q+AFL +GLG +V+GAGGIRPCN++FGADQF+  +E G+  ++SF NW++F F+ A I++
Sbjct: 161 QIAFLLMGLGFLVVGAGGIRPCNLAFGADQFNPKSESGKRGIDSFFNWYFFTFTFAQILS 220

Query: 243 LTLVVYVQTNISWTLGFAIPTAAFVFSVLVFLSGRRFYICKAPQGSVFADMAKVIVATCH 302
           LTLVVYVQ+N+SWT+G  IP      + L+F +G + Y+     GS  A +A+VI     
Sbjct: 221 LTLVVYVQSNVSWTIGLTIPAVLMFLACLIFFAGDKLYVKIKASGSPLAGIAQVIAVAIK 280

Query: 303 KRGIPKPSPD---DLHN--PPMDSDSSKLAHTDRFLVFDKAATVVDSATELDEEGRSKNE 362
           KRG+ KP+     +L+N  PP  + +SKL +TD+F   DKAA ++    +L  +G+  + 
Sbjct: 281 KRGL-KPAKQPWLNLYNYYPPKYA-NSKLKYTDQFRFLDKAA-ILTPEDKLQPDGKPADP 340

Query: 363 WRLCSVHQVEQFKCVVGIVPVWAAGIACFISMQQMSSFGILQAIQMNRFLGP-HFQIPPA 422
           W+LC++ QVE+ KC+V ++P+W A    ++++ Q  ++ + QA+Q +R LG   F IP A
Sbjct: 341 WKLCTMQQVEEVKCIVRVLPIWFASSIYYLTITQQMTYPVFQALQSDRRLGSGGFVIPAA 400

Query: 423 WMSLTPMIALSIWIYIYEK-YVEFMKRKTHHNKRLSMKRRIDIGIVMSVLCMVVAGILEK 482
              +  M  ++++I +Y++  V  M+R T  +  +++ +RI  GI  +   +VVAG +E+
Sbjct: 401 TYVVFLMTGMTVFIVVYDRVLVPTMRRITGLDTGITLLQRIGTGIFFATASLVVAGFVEE 460

Query: 483 VRRDSAL--------ENKSFVSPLHVSVLIPEFVLSGLTEAFAAIAVMELLTTHLPESLR 542
            RR  AL          K  +S +    LIP+  L+G+ EAFAAI  ME      PE++R
Sbjct: 461 RRRTFALTKPTLGMAPRKGEISSMSAMWLIPQLSLAGVAEAFAAIGQMEFYYKQFPENMR 520

Query: 543 TVAGAIFFLSLSLASYLSSVIVGILSKISKKTPWLGGN----DLNKNRLDYFFYVVAVIA 602
           + AG+IF++   ++SYL S ++  + + ++ +   GGN    DLNK RLD F++++A I 
Sbjct: 521 SFAGSIFYVGGGVSSYLGSFLIATVHRTTQNSS--GGNWLAEDLNKGRLDLFYFMIAGIL 580

Query: 603 GLNFFYFRLVAAKFLPKGDVD 604
            +NF YF +++  +  KG  D
Sbjct: 581 AVNFAYFLVMSRWYRYKGSDD 596

BLAST of Spg012481 vs. ExPASy Swiss-Prot
Match: Q9CAR9 (Putative protein NRT1/ PTR FAMILY 2.14 OS=Arabidopsis thaliana OX=3702 GN=NPF2.14 PE=5 SV=1)

HSP 1 Score: 379.0 bits (972), Expect = 1.0e-103
Identity = 209/550 (38.00%), Postives = 337/550 (61.27%), Query Frame = 0

Query: 55  SSSDPPPSKSAHGGWRAVRFILANETFEKLASMSLIANLVLYLHTMYNLDNVDSAYVFQI 114
           SSSD    +    GW+A+ +I+ NET E+LA+  L+AN ++Y+   Y++D V +  +   
Sbjct: 8   SSSDLTTRQRKPLGWKAMPYIIGNETLERLATFGLMANFMVYMVREYHMDQVQAVTLINT 67

Query: 115 WGGTTNFAPLAGAFLADAYLGRFYTLLFGSIASFLGMGVLTLGAGLPQMRPPPCTTGE-- 174
           W   TNFAP+ GAF++D+Y G+F T++FGSIA  LGM VLT  + +P +RPPPCT  +  
Sbjct: 68  WSALTNFAPIIGAFISDSYTGKFNTIVFGSIAELLGMLVLTFTSLVPNLRPPPCTADQIT 127

Query: 175 SNCPQPHPWQLAFLYLGLGLIVIGAGGIRPCNISFGADQFDTNTEKGRAKLESFLNWWYF 234
             C      QL  L  GL L+ +G GGIR C++ F  DQFD +TE+GR    SF +W+Y 
Sbjct: 128 GQCIPYSYSQLYVLLSGLFLLSVGTGGIRSCSVPFSLDQFDDSTEEGREGSRSFFSWYYT 187

Query: 235 LFSIALIIALTLVVYVQTNISWTLGFAIPTAAFVFSVLVFLSGRRFYICKAPQGSVFADM 294
             +I  ++++TLV+YVQ NISW +GFAIPT    F++L+   G R+Y+   P+GSVF+ +
Sbjct: 188 THTIVQLVSMTLVLYVQNNISWGIGFAIPTVLNFFALLLLFVGTRYYVFVKPEGSVFSGV 247

Query: 295 AKVIVATCHKRGIPKPSPDDLHNPPMDSD--SSKLAHTDRFLVFDKAATVVDSATELDEE 354
            KV+VA   KR     S  D H P +++D  S+KL  TD+F   +KA  V+++    DE 
Sbjct: 248 FKVLVAAYKKRKARFTSGIDYHQPLLETDLQSNKLVLTDQFRFLNKAVIVMNN----DEA 307

Query: 355 GRSKNEWRLCSVHQVEQFKCVVGIVPVWAAGIACFISMQQMSSFGILQAIQMN-RFLGPH 414
           G    EWR C+V Q+E  K ++ I+P++A+ I  F++M Q  +F + QA++M+ +F G  
Sbjct: 308 G--NEEWRTCTVRQIEDIKSIISIIPIFASSIIGFLAMNQQQTFTVSQALKMDLQFPGTS 367

Query: 415 FQIPPAWMSLTPMIALSIWIYIYEK-YVEFMKRKTHHNKRLSMKRRIDIGIVMSVLCMVV 474
           + IPPA +++  ++ + IW+  YE   V  ++  T  N  +S+ +++ IG + S+  M++
Sbjct: 368 YLIPPASITVISLLNIGIWLPFYETVLVRHIENITKQNGGISLLQKVGIGNIFSISTMLI 427

Query: 475 AGILEKVRRDSALENKSFVSPLHVSVLIPEFVLSGLTEAFAAIAVMELLTTHLPESLRTV 534
           +GI+E+ RRD +L        + V  L P+ VL G  + F  + + E     +P ++R++
Sbjct: 428 SGIVERKRRDLSLNGVK----MSVFWLTPQQVLMGFYQVFTIVGLTEFFNKQVPINMRSI 487

Query: 535 AGAIFFLSLSLASYLSSVIVGILSKISKK--TPWLGGNDLNKNRLDYFFYVVAVIAGLNF 594
             ++ +L LSLASYLSS +V I+  ++ +    WL  +D++K++LD F+Y +A ++ LNF
Sbjct: 488 GNSLLYLGLSLASYLSSAMVSIVHSVTARGGQSWL-TDDIDKSKLDCFYYFIAALSTLNF 546

Query: 595 FYFRLVAAKF 597
            +F   A ++
Sbjct: 548 IFFFWCARRY 546

BLAST of Spg012481 vs. ExPASy TrEMBL
Match: A0A6J1F0P7 (protein NRT1/ PTR FAMILY 2.8-like OS=Cucurbita moschata OX=3662 GN=LOC111438173 PE=3 SV=1)

HSP 1 Score: 961.4 bits (2484), Expect = 1.8e-276
Identity = 480/579 (82.90%), Postives = 531/579 (91.71%), Query Frame = 0

Query: 47  MENGSLDHSSSDPPPSKSAHGGWRAVRFILANETFEKLASMSLIANLVLYLHTMYNLDNV 106
           M+NG+L  SS DP P K   GGWRAVRFILANETFEKLASMSLIANL+LYLHTMYNLDNV
Sbjct: 1   MDNGTL-RSSLDPSP-KPQLGGWRAVRFILANETFEKLASMSLIANLILYLHTMYNLDNV 60

Query: 107 DSAYVFQIWGGTTNFAPLAGAFLADAYLGRFYTLLFGSIASFLGMGVLTLGAGLPQMRPP 166
           DSAYVFQIWGGTTNF+PLAGAFLADAYLGRFYTLLFGSIASFLGMGVLTLGA LP+MRPP
Sbjct: 61  DSAYVFQIWGGTTNFSPLAGAFLADAYLGRFYTLLFGSIASFLGMGVLTLGAALPEMRPP 120

Query: 167 PCTTGESNCPQPHPWQLAFLYLGLGLIVIGAGGIRPCNISFGADQFDTNTEKGRAKLESF 226
           PC++G+++C Q   WQLAFLYLGLGL+VIGAGGIRPCNISFGADQFDTNTEKGRAKLESF
Sbjct: 121 PCSSGKTDCIQSKSWQLAFLYLGLGLLVIGAGGIRPCNISFGADQFDTNTEKGRAKLESF 180

Query: 227 LNWWYFLFSIALIIALTLVVYVQTNISWTLGFAIPTAAFVFSVLVFLSGRRFYICKAPQG 286
           LNWWYFLFSIAL+IALTLVVY+QTNISWTLGFAIPTAAF+FS+++FL GR  YIC+ PQG
Sbjct: 181 LNWWYFLFSIALVIALTLVVYIQTNISWTLGFAIPTAAFIFSIVIFLLGRHLYICRIPQG 240

Query: 287 SVFADMAKVIVATCHKRGIPKPSPDDLHNPPMDSDSSKLAHTDRFLVFDKAATVVDSATE 346
           SVFADMAKV++AT  KR IP+PSP+ LH+PPM  DSSKL HT+RFLVFDKAA V+DS+ E
Sbjct: 241 SVFADMAKVLIATYRKRQIPRPSPNHLHDPPM--DSSKLPHTNRFLVFDKAAMVIDSSME 300

Query: 347 LDEEGRSKNEWRLCSVHQVEQFKCVVGIVPVWAAGIACFISMQQMSSFGILQAIQMNRFL 406
           LDE+GRSKNEW+LC+VHQVEQ KCVVGIVP+WAAGI+CFISMQQM SFGILQAIQMNRFL
Sbjct: 301 LDEQGRSKNEWKLCTVHQVEQLKCVVGIVPIWAAGISCFISMQQMGSFGILQAIQMNRFL 360

Query: 407 GPHFQIPPAWMSLTPMIALSIWIYIYEKYVEFMKRKTHHNKRLSMKRRIDIGIVMSVLCM 466
           GPHF+IPPAWMSL PMIALS+WIYIYEKYVEFM+RKTHHNKR SMK RI+IGIVMSVLCM
Sbjct: 361 GPHFEIPPAWMSLIPMIALSLWIYIYEKYVEFMRRKTHHNKRFSMKMRIEIGIVMSVLCM 420

Query: 467 VVAGILEKVRRDSALENKSFVSPLHVSVLIPEFVLSGLTEAFAAIAVMELLTTHLPESLR 526
           +VAGILEK+RRDSA+EN++FVSPLHVSVLIPEF LSGLTEAFAAI+VMELLTT LPESLR
Sbjct: 421 IVAGILEKIRRDSAIENRTFVSPLHVSVLIPEFALSGLTEAFAAISVMELLTTQLPESLR 480

Query: 527 TVAGAIFFLSLSLASYLSSVIVGILSKISK----KTPWLGGNDLNKNRLDYFFYVVAVIA 586
           TVAGAIFFLSLSLASYLSSV++GI++++SK    KT WL GNDLNKN LDYFFYVV VIA
Sbjct: 481 TVAGAIFFLSLSLASYLSSVLMGIVNRVSKTGDSKTSWLEGNDLNKNHLDYFFYVVGVIA 540

Query: 587 GLNFFYFRLVAAKFLPKGDVDPRQKAGERQLEDEESGYR 622
            LNFFYFR V  +FLP+GDVDPR KA ER+ EDEESG+R
Sbjct: 541 ALNFFYFRFVGTRFLPEGDVDPRVKASERRSEDEESGFR 575

BLAST of Spg012481 vs. ExPASy TrEMBL
Match: A0A6J1DBD9 (protein NRT1/ PTR FAMILY 2.8-like OS=Momordica charantia OX=3673 GN=LOC111019435 PE=3 SV=1)

HSP 1 Score: 891.7 bits (2303), Expect = 1.7e-255
Identity = 454/585 (77.61%), Postives = 504/585 (86.15%), Query Frame = 0

Query: 47  MENGSLDHSSSDPP---PSKSAHGGWRAVRFILANETFEKLASMSLIANLVLYLHTMYNL 106
           MEN ++DHSS DPP   PSK+A GGWRAVRFILANETFEKLASMSL+ANL+LYLHTMYNL
Sbjct: 1   MENDAIDHSSLDPPPVAPSKAAVGGWRAVRFILANETFEKLASMSLVANLILYLHTMYNL 60

Query: 107 DNVDSAYVFQIWGGTTNFAPLAGAFLADAYLGRFYTLLFGSIASFLGMGVLTLGAGLPQM 166
           DNV SA V  IWGGTTNFAP+AGAFLADAYLGRFYTLLFGSIASF GMGVLTLGAGLP++
Sbjct: 61  DNVASANVINIWGGTTNFAPIAGAFLADAYLGRFYTLLFGSIASFFGMGVLTLGAGLPEL 120

Query: 167 RPPPCTTGESNCPQPHPWQLAFLYLGLGLIVIGAGGIRPCNISFGADQFDTNTEKGRAKL 226
           RPP C+  ++ CPQP PWQLAFLYLGLGL+VIGAGGIRPCNISFGADQFDT T KGRAKL
Sbjct: 121 RPPSCSHSQAKCPQPQPWQLAFLYLGLGLLVIGAGGIRPCNISFGADQFDTTTYKGRAKL 180

Query: 227 ESFLNWWYFLFSIALIIALTLVVYVQTNISWTLGFAIPTAAFVFSVLVFLSGRRFYICKA 286
           ESFLNWWYFLFSIALI+ALT+VVY+QTNISW  GFAIPTA FV S+L+FL GR  YI K 
Sbjct: 181 ESFLNWWYFLFSIALIVALTVVVYIQTNISWVTGFAIPTACFVLSILIFLLGRGTYIRKT 240

Query: 287 PQGSVFADMAKVIVATCHKRGIPKP-SPDDLHNPPMD-SDSSKLAHTDRFLVFDKAATVV 346
           PQGSVFADMAKVI+ATC K  + KP + ++LH+PP    DS+KL HT+RFLVFDKAATVV
Sbjct: 241 PQGSVFADMAKVIIATCRKSKLSKPNNQNNLHDPPPTIPDSTKLPHTNRFLVFDKAATVV 300

Query: 347 DSATELDEEGRSKNEWRLCSVHQVEQFKCVVGIVPVWAAGIACFISMQQMSSFGILQAIQ 406
           D   ELDE G S + WRLCSV QVEQ KCV+GIVPVW AGI+CFI+MQQM SFG+LQAIQ
Sbjct: 301 DPVAELDEHGNSVSHWRLCSVQQVEQLKCVIGIVPVWVAGISCFIAMQQMGSFGVLQAIQ 360

Query: 407 MNRFLGPHFQIPPAWMSLTPMIALSIWIYIYEKYVEFMKRKTHHNKRLSMKRRIDIGIVM 466
           M+RFLG HF+IPPAWM+LTPMIALSIWI++YEKYV  MKRKT  NKR+SM+RRI+IGIVM
Sbjct: 361 MDRFLGRHFEIPPAWMNLTPMIALSIWIFVYEKYVAMMKRKTGQNKRISMQRRIEIGIVM 420

Query: 467 SVLCMVVAGILEKVRRDSALENKSFVSPLHVSVLIPEFVLSGLTEAFAAIAVMELLTTHL 526
           SV+CMVV+GI EK+RRD A+ N SFV+ LHV+ L PEF LSGLTEAFAAIAVMELLTTHL
Sbjct: 421 SVVCMVVSGITEKLRRDLAVGNGSFVARLHVTALXPEFALSGLTEAFAAIAVMELLTTHL 480

Query: 527 PESLRTVAGAIFFLSLSLASYLSSVIVGILSKIS-----KKTPWLGGNDLNKNRLDYFFY 586
           PESLRTVAGAIFFLSLSLASYLSSV+VGI+SKIS     KKT W+GGNDLN NRL+YFFY
Sbjct: 481 PESLRTVAGAIFFLSLSLASYLSSVLVGIISKISTTIDGKKTAWIGGNDLNHNRLEYFFY 540

Query: 587 VVAVIAGLNFFYFRLVAAKFLPKGDVDPRQKAGERQLEDEESGYR 622
           VVAVI  LNFFYFR  AA FLPK DVD   +  ERQLEDEE+G R
Sbjct: 541 VVAVIGTLNFFYFRFFAAGFLPKSDVDDHDQTHERQLEDEETGRR 585

BLAST of Spg012481 vs. ExPASy TrEMBL
Match: A0A1S4DX14 (protein NRT1/ PTR FAMILY 2.8-like OS=Cucumis melo OX=3656 GN=LOC103491761 PE=3 SV=1)

HSP 1 Score: 797.3 bits (2058), Expect = 4.4e-227
Identity = 397/472 (84.11%), Postives = 431/472 (91.31%), Query Frame = 0

Query: 151 MGVLTLGAGLPQMRPPPCTTGESNCPQPHPWQLAFLYLGLGLIVIGAGGIRPCNISFGAD 210
           MGVLTLGAGLPQMRPPPC +GESNCPQP PWQL+FLYLGLGLIVIGAGGIRPCNISFGAD
Sbjct: 1   MGVLTLGAGLPQMRPPPCISGESNCPQPLPWQLSFLYLGLGLIVIGAGGIRPCNISFGAD 60

Query: 211 QFDTNTEKGRAKLESFLNWWYFLFSIALIIALTLVVYVQTNISWTLGFAIPTAAFVFSVL 270
           QFDTNTEKGRAKLESFLNWWYFLFS+AL+IALT+VVYVQTN+SWTLGF IPTA FVFS+ 
Sbjct: 61  QFDTNTEKGRAKLESFLNWWYFLFSVALVIALTIVVYVQTNVSWTLGFGIPTACFVFSIS 120

Query: 271 VFLSGRRFYICKAPQGSVFADMAKVIVATCHKRGIPKPSP-DDLHNPPMDSDSSKLAHTD 330
           +FL G R+YICKAPQGSVFAD+AKV+VATC KR IPKP+  D LHNPPM  +SSKLAHT+
Sbjct: 121 IFLLGHRYYICKAPQGSVFADIAKVVVATCRKRQIPKPTQFDQLHNPPM--NSSKLAHTN 180

Query: 331 RFLVFDKAATVVDSATELDEEGRSKNEWRLCSVHQVEQFKCVVGIVPVWAAGIACFISMQ 390
           RFL+FDKAATVVDS+ ELDE+G+SKNEWRLCSVHQVE+FKCVVGI+PVW AGI CF+SMQ
Sbjct: 181 RFLIFDKAATVVDSSVELDEDGKSKNEWRLCSVHQVEEFKCVVGIIPVWLAGIPCFMSMQ 240

Query: 391 QMSSFGILQAIQMNRFLGPHFQIPPAWMSLTPMIALSIWIYIYEKYVEFMKRKTHHNKRL 450
           QM SFGILQAIQMNRF+GPHFQIPPAWMSLTPMI LSIWIYIYEKYVE MK+KT  NKR 
Sbjct: 241 QMGSFGILQAIQMNRFIGPHFQIPPAWMSLTPMITLSIWIYIYEKYVEHMKKKTRSNKRF 300

Query: 451 SMKRRIDIGIVMSVLCMVVAGILEKVRRDSALENKSFVSPLHVSVLIPEFVLSGLTEAFA 510
           SMK RI+IGIVMSVLCMVVAGILEK+RRD+A+ENKSF+SPLHV VLIPEF LSGLTEAFA
Sbjct: 301 SMKTRIEIGIVMSVLCMVVAGILEKLRRDAAVENKSFISPLHVWVLIPEFALSGLTEAFA 360

Query: 511 AIAVMELLTTHLPESLRTVAGAIFFLSLSLASYLSSVIVGILSKISKKTPWLGGNDLNKN 570
           AIAVMELLTTHLPESLRTVAGAIFFLSLSLASYLSSV+ GI+SK+ ++  WLGGNDLNKN
Sbjct: 361 AIAVMELLTTHLPESLRTVAGAIFFLSLSLASYLSSVLTGIVSKVERE--WLGGNDLNKN 420

Query: 571 RLDYFFYVVAVIAGLNFFYFRLVAAKFLPKGDVDPRQKAGERQLEDEESGYR 622
           RLDYFFYVV VIA LNFFYFR  A  FLPK DVDPRQKA +RQLED E G R
Sbjct: 421 RLDYFFYVVGVIAALNFFYFRFFAGAFLPKPDVDPRQKAHQRQLEDAELGRR 468

BLAST of Spg012481 vs. ExPASy TrEMBL
Match: A0A6J1HNI7 (protein NRT1/ PTR FAMILY 2.8-like OS=Cucurbita maxima OX=3661 GN=LOC111466257 PE=3 SV=1)

HSP 1 Score: 792.3 bits (2045), Expect = 1.4e-225
Identity = 390/475 (82.11%), Postives = 435/475 (91.58%), Query Frame = 0

Query: 151 MGVLTLGAGLPQMRPPPCTTGESNCPQPHPWQLAFLYLGLGLIVIGAGGIRPCNISFGAD 210
           MGVLTLGA LP+MRPPPC++G+++C Q  PWQLAFLYLGLGL+VIGAGGIRPCNISFGAD
Sbjct: 1   MGVLTLGAALPEMRPPPCSSGKTDCIQSKPWQLAFLYLGLGLLVIGAGGIRPCNISFGAD 60

Query: 211 QFDTNTEKGRAKLESFLNWWYFLFSIALIIALTLVVYVQTNISWTLGFAIPTAAFVFSVL 270
           QFDTNTEKGRAKLESFLNWWYFLFSIAL+IALTLVVY+QTNISWTLGFAIPTAAF+FS++
Sbjct: 61  QFDTNTEKGRAKLESFLNWWYFLFSIALVIALTLVVYIQTNISWTLGFAIPTAAFIFSIV 120

Query: 271 VFLSGRRFYICKAPQGSVFADMAKVIVATCHKRGIPKPSPDDLHNPPMDSDSSKLAHTDR 330
           +FL GR  YIC+ PQGSVFADMAKV++AT  KR IP+PSP+ LH+PPM  DSSKL HT+R
Sbjct: 121 IFLLGRHLYICRIPQGSVFADMAKVLIATYRKRQIPRPSPNHLHDPPM--DSSKLPHTNR 180

Query: 331 FLVFDKAATVVDSATELDEEGRSKNEWRLCSVHQVEQFKCVVGIVPVWAAGIACFISMQQ 390
           FLVFDKAA V DS+ ELDE+GRSKNEW+LC+VHQVEQ KCVVGIVP+WAAGI+CFISMQQ
Sbjct: 181 FLVFDKAAMVTDSSMELDEQGRSKNEWKLCTVHQVEQLKCVVGIVPIWAAGISCFISMQQ 240

Query: 391 MSSFGILQAIQMNRFLGPHFQIPPAWMSLTPMIALSIWIYIYEKYVEFMKRKTHHNKRLS 450
           M SFGILQAIQMNRFLGPHF+IPPAWMSL PMIALS+WIYIYEKYVEFMKRKTHHNKR S
Sbjct: 241 MGSFGILQAIQMNRFLGPHFEIPPAWMSLIPMIALSLWIYIYEKYVEFMKRKTHHNKRFS 300

Query: 451 MKRRIDIGIVMSVLCMVVAGILEKVRRDSALENKSFVSPLHVSVLIPEFVLSGLTEAFAA 510
           MK RI+IGI+MSVLCMVVAGILEK+RRDSA+EN++FVSPLHVSVLIPEF LSGLTEAFAA
Sbjct: 301 MKMRIEIGIMMSVLCMVVAGILEKIRRDSAIENRTFVSPLHVSVLIPEFALSGLTEAFAA 360

Query: 511 IAVMELLTTHLPESLRTVAGAIFFLSLSLASYLSSVIVGILSKISK----KTPWLGGNDL 570
           I+VMELLTT LPESLRTVAGAIFFLSLSLASYLSSV++GI++++SK    KT WL GNDL
Sbjct: 361 ISVMELLTTQLPESLRTVAGAIFFLSLSLASYLSSVLMGIVNRVSKTEDSKTSWLEGNDL 420

Query: 571 NKNRLDYFFYVVAVIAGLNFFYFRLVAAKFLPKGDVDPRQKAGERQLEDEESGYR 622
           NKN LDYFFYVV VIA LNFFYFR V  +FLP+GDVDPR KA ER+ EDEESG+R
Sbjct: 421 NKNHLDYFFYVVGVIAALNFFYFRFVGTRFLPEGDVDPRVKANERRSEDEESGFR 473

BLAST of Spg012481 vs. ExPASy TrEMBL
Match: A0A6J1GFB3 (protein NRT1/ PTR FAMILY 2.8-like OS=Cucurbita moschata OX=3662 GN=LOC111453646 PE=3 SV=1)

HSP 1 Score: 770.0 bits (1987), Expect = 7.5e-219
Identity = 384/458 (83.84%), Postives = 416/458 (90.83%), Query Frame = 0

Query: 151 MGVLTLGAGLPQMRPPPCTTGESNCPQPHPWQLAFLYLGLGLIVIGAGGIRPCNISFGAD 210
           MGVLTLGAG P+MRPPPC  GESNCPQPHPWQL FLYLGLGL+VIGAGGIRPCNISFGAD
Sbjct: 1   MGVLTLGAGFPEMRPPPCRHGESNCPQPHPWQLGFLYLGLGLLVIGAGGIRPCNISFGAD 60

Query: 211 QFDTNTEKGRAKLESFLNWWYFLFSIALIIALTLVVYVQTNISWTLGFAIPTAAFVFSVL 270
           QFDTNTEKGRAKLESFLNWWY LFS+AL+IALTLVVYVQT+ISWTLGFAIPTA FVFS+L
Sbjct: 61  QFDTNTEKGRAKLESFLNWWYLLFSVALVIALTLVVYVQTSISWTLGFAIPTACFVFSIL 120

Query: 271 VFLSGRRFYICKAPQGSVFADMAKVIVATCHKRGIPKPSPDD--LHNPPMDSDSSKLAHT 330
           +FL GRRFYICK PQGSVFAD+AKV+VATC KR +PK +  D  LH+PPM  DSSKL+HT
Sbjct: 121 IFLLGRRFYICKTPQGSVFADIAKVLVATCRKRNVPKFTQIDHFLHDPPM--DSSKLSHT 180

Query: 331 DRFLVFDKAATVVDSATELDEEGRSKNEWRLCSVHQVEQFKCVVGIVPVWAAGIACFISM 390
           DRFLVFDKAATVVDS+TELDE+G+SKNEW+LCSVHQVEQFKCVVGIVP+WAAGI CFISM
Sbjct: 181 DRFLVFDKAATVVDSSTELDEQGKSKNEWKLCSVHQVEQFKCVVGIVPIWAAGIPCFISM 240

Query: 391 QQMSSFGILQAIQMNRFLGPHFQIPPAWMSLTPMIALSIWIYIYEKYVEFMKRKTHHNKR 450
           QQM SFGILQAIQMNRF+GPHFQIPPAWMSLTPMIALSIWIYIYEKYVEFMK+KTH NKR
Sbjct: 241 QQMGSFGILQAIQMNRFIGPHFQIPPAWMSLTPMIALSIWIYIYEKYVEFMKKKTHKNKR 300

Query: 451 LSMKRRIDIGIVMSVLCMVVAGILEKVRRDSALENKSFVSPLHVSVLIPEFVLSGLTEAF 510
            S+K RI+IGIVMSVLCMVVAGILE +RR SA ENKSFVSPLHV +LIPEF LSGLTEAF
Sbjct: 301 FSLKTRIEIGIVMSVLCMVVAGILENIRRSSAEENKSFVSPLHVWMLIPEFALSGLTEAF 360

Query: 511 AAIAVMELLTTHLPESLRTVAGAIFFLSLSLASYLSSVIVGILSKISK----KTPWLGGN 570
           AAIAVMELLTTHLPESLRTVAGAIFFLSLSL SY+SSV++ I+SKI++    K+ WLGGN
Sbjct: 361 AAIAVMELLTTHLPESLRTVAGAIFFLSLSLGSYMSSVLMAIVSKINRVEGGKSGWLGGN 420

Query: 571 DLNKNRLDYFFYVVAVIAGLNFFYFRLVAAKFLPKGDV 603
           DLNKNRLDYFFYVV VIA  NFFYFR  A  FLPK +V
Sbjct: 421 DLNKNRLDYFFYVVGVIAAFNFFYFRFFATHFLPKPNV 456

BLAST of Spg012481 vs. TAIR 10
Match: AT5G28470.1 (Major facilitator superfamily protein )

HSP 1 Score: 567.0 bits (1460), Expect = 1.9e-161
Identity = 291/554 (52.53%), Postives = 385/554 (69.49%), Query Frame = 0

Query: 52  LDHSSSDPPPS---KSAHGGWRAVRFILANETFEKLASMSLIANLVLYLHTMYNLDNVDS 111
           +D  SS P      K   GGWRA+++I+ANE+FEKLASMSLI NL +YL T YNL  V  
Sbjct: 1   MDVESSSPSSHALIKKEKGGWRAIKYIIANESFEKLASMSLIGNLSVYLMTKYNLGGVFL 60

Query: 112 AYVFQIWGGTTNFAPLAGAFLADAYLGRFYTLLFGSIASFLGMGVLTLGAGLPQMRPPPC 171
             V  IW G+ N   LAGAF++DAYLGRF+TLL GSIASF+GMG+  L A LP +RP  C
Sbjct: 61  VNVINIWFGSCNILTLAGAFVSDAYLGRFWTLLLGSIASFIGMGIFALTAALPSLRPDAC 120

Query: 172 TTGESNCPQPHPWQLAFLYLGLGLIVIGAGGIRPCNISFGADQFDTNTEKGRAKLESFLN 231
               +   QP  WQL  L+ GLGL+ IGAGG+RPCNI+FGADQFDT+T+KG+A LE+F N
Sbjct: 121 IDPSNCSNQPAKWQLGVLFSGLGLLAIGAGGVRPCNIAFGADQFDTSTKKGKAHLETFFN 180

Query: 232 WWYFLFSIALIIALTLVVYVQTNISWTLGFAIPTAAFVFSVLVFLSGRRFYICKAPQGSV 291
           WWYF F++AL+IALT VVY+QTNISW +GF IPTA    S+  F+ G+  YIC   +GSV
Sbjct: 181 WWYFSFTVALVIALTGVVYIQTNISWVIGFVIPTACLALSITTFVIGQHTYICAKAEGSV 240

Query: 292 FADMAKVIVATCHKRGIPKPSPDDLH--NPPMDSDSSKLAHTDRFLVFDKAATVVDSATE 351
           FAD+ KV+ A C KR + KP  D      P  D   + L      L F   A++V +  E
Sbjct: 241 FADIVKVVTAACKKRKV-KPGSDITFYIGPSNDGSPTTLVRDKHRLRFFDKASIVTNPNE 300

Query: 352 LDEEGRSKNEWRLCSVHQVEQFKCVVGIVPVWAAGIACFISMQQMSSFGILQAIQMNRFL 411
           L+E+G +K +WRLCSV QV+  KCV  I+PVW  GIACFI   Q + +GILQA+QM++  
Sbjct: 301 LNEDGNAKYKWRLCSVQQVKNLKCVTAILPVWVTGIACFILTDQQNIYGILQAMQMDKTF 360

Query: 412 GPH-FQIPPAWMSLTPMIALSIWIYIYE-KYVEFMKRKTHHNKRLSMKRRIDIGIVMSVL 471
           GPH FQ+P  WM+L  MI L+IWI +YE   +  +K+ T   KRL++K RI+  IVM ++
Sbjct: 361 GPHNFQVPAGWMNLVSMITLAIWISLYECVIIPIVKQITGRKKRLTLKHRIE--IVMGII 420

Query: 472 CMVVAGILEKVRRDSALENKSFVSPLHVSVLIPEFVLSGLTEAFAAIAVMELLTTHLPES 531
           CM+VAG  EK RR SAL+N SFVSP+ + +L+P+F L+GLTEAF+A+A+ME LT  +PE 
Sbjct: 421 CMIVAGFQEKKRRASALKNGSFVSPVSIVMLLPQFALAGLTEAFSAVALMEFLTVRMPEH 480

Query: 532 LRTVAGAIFFLSLSLASYLSSVIVGILSKISKK--TPWLGGNDLNKNRLDYFFYVVAVIA 591
           +R VAGAIFFLS S+ASY+ ++++ ++  +++K    WLG  DLNKNRL+ +F+++A I 
Sbjct: 481 MRAVAGAIFFLSSSIASYICTLLINVIDAVTRKEGKSWLGDKDLNKNRLENYFFIIAGIQ 540

Query: 592 GLNFFYFRLVAAKF 597
             N  YFRL A+++
Sbjct: 541 VANLLYFRLFASRY 551

BLAST of Spg012481 vs. TAIR 10
Match: AT1G69870.1 (nitrate transporter 1.7 )

HSP 1 Score: 424.9 bits (1091), Expect = 1.1e-118
Identity = 227/569 (39.89%), Postives = 360/569 (63.27%), Query Frame = 0

Query: 67  GGWRAVRFILANETFEKLASMSLIANLVLYLHTMYNLDNVDSAYVFQIWGGTTNFAPLAG 126
           GGWRAV FIL NET E+L S+ L+AN ++YL  +++L+ VD+A V  IW G TN  PL G
Sbjct: 53  GGWRAVSFILGNETLERLGSIGLLANFMVYLTKVFHLEQVDAANVINIWSGFTNLTPLVG 112

Query: 127 AFLADAYLGRFYTLLFGSIASFLGMGVLTLGAGLPQMRPPPCTTGES-NCPQPHPWQLAF 186
           A+++D Y+GRF T+ F S A+ LG+  +TL A  PQ+ P  C + +  +C  P+  Q+  
Sbjct: 113 AYISDTYVGRFKTIAFASFATLLGLITITLTASFPQLHPASCNSQDPLSCGGPNKLQIGV 172

Query: 187 LYLGLGLIVIGAGGIRPCNISFGADQFDTNTEKGRAKLESFLNWWYFLFSIALIIALTLV 246
           L LGL  + +G+GGIRPC+I FG DQFD  TE+G   + SF NW+Y  F++ LII  T+V
Sbjct: 173 LLLGLCFLSVGSGGIRPCSIPFGVDQFDQRTEEGVKGVASFFNWYYMTFTVVLIITQTVV 232

Query: 247 VYVQTNISWTLGFAIPTAAFVFSVLVFLSGRRFYICKAPQGSVFADMAKVIVATCHKRGI 306
           VY+Q  +SW +GF+IPT     +V++F +G + Y+   P+GS+F+ +A+VIVA   KR +
Sbjct: 233 VYIQDQVSWIIGFSIPTGLMALAVVMFFAGMKRYVYVKPEGSIFSGIAQVIVAARKKRKL 292

Query: 307 PKPSPDD----LHNPPMDSD-SSKLAHTDRFLVFDKAATVVDSATELDEEGRSKNEWRLC 366
             P+ DD     ++P + S   SKL  +++F   DKAA V++   +L  EG   ++WRLC
Sbjct: 293 KLPAEDDGTVTYYDPAIKSSVLSKLHRSNQFRCLDKAAVVIEG--DLTPEGPPADKWRLC 352

Query: 367 SVHQVEQFKCVVGIVPVWAAGIACFISMQQMSSFGILQAIQMNRFLGPHFQIPPAWMSLT 426
           SV +VE+ KC++ IVP+W+AGI    +M    +F + QA++M+R LGP F+IP   +S+ 
Sbjct: 353 SVQEVEEVKCLIRIVPIWSAGIISLAAMTTQGTFTVSQALKMDRNLGPKFEIPAGSLSVI 412

Query: 427 PMIALSIWIYIYEK-YVEFMKRKTHHNKRLSMKRRIDIGIVMSVLCMVVAGILEKVRRDS 486
            ++ + I++  Y++ +V FM+R T H   +++ +RI  GIV ++  M+VAGI+E++RR  
Sbjct: 413 SLLTIGIFLPFYDRVFVPFMRRITGHKSGITLLQRIGTGIVFAIFSMIVAGIVERMRRIR 472

Query: 487 ALE--NKSFVSPLHVSVLIPEFVLSGLTEAFAAIAVMELLTTHLPESLRTVAGAIFFLSL 546
           ++   + + ++P+ V  L P+ +L GL EAF  I  +E   +  PE +R++A ++F LS 
Sbjct: 473 SINAGDPTGMTPMSVFWLSPQLILMGLCEAFNIIGQIEFFNSQFPEHMRSIANSLFSLSF 532

Query: 547 SLASYLSSVIVGILSKIS---KKTPWLGGNDLNKNRLDYFFYVVAVIAGLNFFYFRLVAA 606
           + +SYLSS +V ++ K S    +  WL  N LN  +LDYF+Y++AV+  +N  YF   A 
Sbjct: 533 AGSSYLSSFLVTVVHKFSGGHDRPDWLNKN-LNAGKLDYFYYLIAVLGVVNLVYFWYCAR 592

Query: 607 KF-----LPKGDVDPRQKAGERQLEDEES 619
            +     LP  D +  + + + ++  ++S
Sbjct: 593 GYRYKVGLPIEDFEEDKSSDDVEMTSKKS 618

BLAST of Spg012481 vs. TAIR 10
Match: AT1G27080.1 (nitrate transporter 1.6 )

HSP 1 Score: 423.7 bits (1088), Expect = 2.5e-118
Identity = 238/565 (42.12%), Postives = 353/565 (62.48%), Query Frame = 0

Query: 67  GGWRAVRFILANETFEKLASMSLIANLVLYLHTMYNLDNVDSAYVFQIWGGTTNFAPLAG 126
           GGWRA+ FIL NET EKL S+ + AN +LYL  +++++ V++  V+ +W G TNFAPL G
Sbjct: 16  GGWRAITFILGNETLEKLGSIGVSANFMLYLRNVFHMEPVEAFNVYYLWMGLTNFAPLLG 75

Query: 127 AFLADAYLGRFYTLLFGSIASFLGMGVLTLGAGLPQMRPPPCTTGE-SNCPQPHPWQLAF 186
           A ++DAY+GRF T+ + S+ S LG+  +TL A LPQ+ PPPC       C  P+  QL  
Sbjct: 76  ALISDAYIGRFKTIAYASLFSILGLMTVTLTACLPQLHPPPCNNPHPDECDDPNKLQLGI 135

Query: 187 LYLGLGLIVIGAGGIRPCNISFGADQFDTNTEKGRAKLESFLNWWYFLFSIALIIALTLV 246
           L+LGLG + IG+GGIRPC+I FG DQFD  TE+G   + SF NW+Y   ++ LI + T+V
Sbjct: 136 LFLGLGFLSIGSGGIRPCSIPFGVDQFDQRTEQGLKGVASFFNWYYLTLTMVLIFSHTVV 195

Query: 247 VYVQTNISWTLGFAIPTAAFVFSVLVFLSGRRFYICKAPQGSVFADMAKVIVATCHKRGI 306
           VY+QT +SW +GF+IPT+    +V++F  G RFY+   P+GSVF+ +A+VIVA   KR +
Sbjct: 196 VYLQT-VSWVIGFSIPTSLMACAVVLFFVGMRFYVYVKPEGSVFSGIARVIVAARKKRDL 255

Query: 307 PKPSPDD----LHNPPMDSD-SSKLAHTDRFLVFDKAATVVDSATELDEEGRSKNEWRLC 366
                DD     + PP+     SKL  TD+F   DKAA ++D   +L  EG   N+WRLC
Sbjct: 256 KISLVDDGTEEYYEPPVKPGVLSKLPLTDQFKFLDKAAVILDG--DLTSEGVPANKWRLC 315

Query: 367 SVHQVEQFKCVVGIVPVWAAGIACFISMQQMSSFGILQAIQMNRFLGPHFQIPPAWMSLT 426
           S+ +VE+ KC++ +VPVW+AGI   ++M   ++F + QA +M+R +GPHF+IP A +++ 
Sbjct: 316 SIQEVEEVKCLIRVVPVWSAGIISIVAMTTQATFMVFQATKMDRHMGPHFEIPAASITVI 375

Query: 427 PMIALSIWIYIYEK-YVEFMKRKTHHNKRLSMKRRIDIGIVMSVLCMVVAGILEKVRRDS 486
             I + IW+ IYE   V F+ R      R+++ +R+ IGIV ++L M  AG +E VRR  
Sbjct: 376 SYITIGIWVPIYEHLLVPFLWRM--RKFRVTLLQRMGIGIVFAILSMFTAGFVEGVRRTR 435

Query: 487 ALENKSFVSPLHVSVLIPEFVLSGLTEAFAAIAVMELLTTHLPESLRTVAGAIFFLSLSL 546
           A E    ++ + V  L    +L GL E+F  I ++E   +  PE +R++A ++F LS + 
Sbjct: 436 ATE----MTQMSVFWLALPLILMGLCESFNFIGLIEFFNSQFPEHMRSIANSLFPLSFAA 495

Query: 547 ASYLSSVIVGILSKIS--KKTP-WLGGNDLNKNRLDYFFYVVAVIAGLNFFYFRLVAAKF 606
           A+YLSS++V  + K+S  K  P WL   DL++ +LDYF+Y++AV+  +N  YF   A ++
Sbjct: 496 ANYLSSLLVTTVHKVSGTKDHPDWL-NKDLDRGKLDYFYYLIAVLGVVNLVYFWYCAHRY 555

Query: 607 LPKGDVDPRQKAGERQLEDEESGYR 622
             K          E+ L D E   R
Sbjct: 556 QYKAGSQIEDFNEEKSLLDIEPNQR 570

BLAST of Spg012481 vs. TAIR 10
Match: AT5G62680.1 (Major facilitator superfamily protein )

HSP 1 Score: 383.3 bits (983), Expect = 3.8e-106
Identity = 216/561 (38.50%), Postives = 341/561 (60.78%), Query Frame = 0

Query: 63  KSAHGGWRAVRFILANETFEKLASMSLIANLVLYLHTMYNLDNVDSAYVFQIWGGTTNFA 122
           K  + GW+ + FI+ NETFEKL  +  ++NL++YL  ++NL ++ +A +   + GT NF 
Sbjct: 41  KVVYRGWKVMPFIIGNETFEKLGIIGTLSNLLVYLTAVFNLKSITAATIINAFSGTINFG 100

Query: 123 PLAGAFLADAYLGRFYTLLFGSIASFLGMGVLTLGAGLPQMRPPPC-TTGESNCPQPHPW 182
               AFL D Y GR+ TL    IA FLG  V+ L A +PQ+ P  C T  +S C  P   
Sbjct: 101 TFVAAFLCDTYFGRYKTLSVAVIACFLGSFVILLTAAVPQLHPAACGTAADSICNGPSGG 160

Query: 183 QLAFLYLGLGLIVIGAGGIRPCNISFGADQFDTNTEKGRAKLESFLNWWYFLFSIALIIA 242
           Q+AFL +GLG +V+GAGGIRPCN++FGADQF+  +E G+  ++SF NW++F F+ A I++
Sbjct: 161 QIAFLLMGLGFLVVGAGGIRPCNLAFGADQFNPKSESGKRGIDSFFNWYFFTFTFAQILS 220

Query: 243 LTLVVYVQTNISWTLGFAIPTAAFVFSVLVFLSGRRFYICKAPQGSVFADMAKVIVATCH 302
           LTLVVYVQ+N+SWT+G  IP      + L+F +G + Y+     GS  A +A+VI     
Sbjct: 221 LTLVVYVQSNVSWTIGLTIPAVLMFLACLIFFAGDKLYVKIKASGSPLAGIAQVIAVAIK 280

Query: 303 KRGIPKPSPD---DLHN--PPMDSDSSKLAHTDRFLVFDKAATVVDSATELDEEGRSKNE 362
           KRG+ KP+     +L+N  PP  + +SKL +TD+F   DKAA ++    +L  +G+  + 
Sbjct: 281 KRGL-KPAKQPWLNLYNYYPPKYA-NSKLKYTDQFRFLDKAA-ILTPEDKLQPDGKPADP 340

Query: 363 WRLCSVHQVEQFKCVVGIVPVWAAGIACFISMQQMSSFGILQAIQMNRFLGP-HFQIPPA 422
           W+LC++ QVE+ KC+V ++P+W A    ++++ Q  ++ + QA+Q +R LG   F IP A
Sbjct: 341 WKLCTMQQVEEVKCIVRVLPIWFASSIYYLTITQQMTYPVFQALQSDRRLGSGGFVIPAA 400

Query: 423 WMSLTPMIALSIWIYIYEK-YVEFMKRKTHHNKRLSMKRRIDIGIVMSVLCMVVAGILEK 482
              +  M  ++++I +Y++  V  M+R T  +  +++ +RI  GI  +   +VVAG +E+
Sbjct: 401 TYVVFLMTGMTVFIVVYDRVLVPTMRRITGLDTGITLLQRIGTGIFFATASLVVAGFVEE 460

Query: 483 VRRDSAL--------ENKSFVSPLHVSVLIPEFVLSGLTEAFAAIAVMELLTTHLPESLR 542
            RR  AL          K  +S +    LIP+  L+G+ EAFAAI  ME      PE++R
Sbjct: 461 RRRTFALTKPTLGMAPRKGEISSMSAMWLIPQLSLAGVAEAFAAIGQMEFYYKQFPENMR 520

Query: 543 TVAGAIFFLSLSLASYLSSVIVGILSKISKKTPWLGGN----DLNKNRLDYFFYVVAVIA 602
           + AG+IF++   ++SYL S ++  + + ++ +   GGN    DLNK RLD F++++A I 
Sbjct: 521 SFAGSIFYVGGGVSSYLGSFLIATVHRTTQNSS--GGNWLAEDLNKGRLDLFYFMIAGIL 580

Query: 603 GLNFFYFRLVAAKFLPKGDVD 604
            +NF YF +++  +  KG  D
Sbjct: 581 AVNFAYFLVMSRWYRYKGSDD 596

BLAST of Spg012481 vs. TAIR 10
Match: AT1G69860.1 (Major facilitator superfamily protein )

HSP 1 Score: 379.0 bits (972), Expect = 7.2e-105
Identity = 209/550 (38.00%), Postives = 337/550 (61.27%), Query Frame = 0

Query: 55  SSSDPPPSKSAHGGWRAVRFILANETFEKLASMSLIANLVLYLHTMYNLDNVDSAYVFQI 114
           SSSD    +    GW+A+ +I+ NET E+LA+  L+AN ++Y+   Y++D V +  +   
Sbjct: 8   SSSDLTTRQRKPLGWKAMPYIIGNETLERLATFGLMANFMVYMVREYHMDQVQAVTLINT 67

Query: 115 WGGTTNFAPLAGAFLADAYLGRFYTLLFGSIASFLGMGVLTLGAGLPQMRPPPCTTGE-- 174
           W   TNFAP+ GAF++D+Y G+F T++FGSIA  LGM VLT  + +P +RPPPCT  +  
Sbjct: 68  WSALTNFAPIIGAFISDSYTGKFNTIVFGSIAELLGMLVLTFTSLVPNLRPPPCTADQIT 127

Query: 175 SNCPQPHPWQLAFLYLGLGLIVIGAGGIRPCNISFGADQFDTNTEKGRAKLESFLNWWYF 234
             C      QL  L  GL L+ +G GGIR C++ F  DQFD +TE+GR    SF +W+Y 
Sbjct: 128 GQCIPYSYSQLYVLLSGLFLLSVGTGGIRSCSVPFSLDQFDDSTEEGREGSRSFFSWYYT 187

Query: 235 LFSIALIIALTLVVYVQTNISWTLGFAIPTAAFVFSVLVFLSGRRFYICKAPQGSVFADM 294
             +I  ++++TLV+YVQ NISW +GFAIPT    F++L+   G R+Y+   P+GSVF+ +
Sbjct: 188 THTIVQLVSMTLVLYVQNNISWGIGFAIPTVLNFFALLLLFVGTRYYVFVKPEGSVFSGV 247

Query: 295 AKVIVATCHKRGIPKPSPDDLHNPPMDSD--SSKLAHTDRFLVFDKAATVVDSATELDEE 354
            KV+VA   KR     S  D H P +++D  S+KL  TD+F   +KA  V+++    DE 
Sbjct: 248 FKVLVAAYKKRKARFTSGIDYHQPLLETDLQSNKLVLTDQFRFLNKAVIVMNN----DEA 307

Query: 355 GRSKNEWRLCSVHQVEQFKCVVGIVPVWAAGIACFISMQQMSSFGILQAIQMN-RFLGPH 414
           G    EWR C+V Q+E  K ++ I+P++A+ I  F++M Q  +F + QA++M+ +F G  
Sbjct: 308 G--NEEWRTCTVRQIEDIKSIISIIPIFASSIIGFLAMNQQQTFTVSQALKMDLQFPGTS 367

Query: 415 FQIPPAWMSLTPMIALSIWIYIYEK-YVEFMKRKTHHNKRLSMKRRIDIGIVMSVLCMVV 474
           + IPPA +++  ++ + IW+  YE   V  ++  T  N  +S+ +++ IG + S+  M++
Sbjct: 368 YLIPPASITVISLLNIGIWLPFYETVLVRHIENITKQNGGISLLQKVGIGNIFSISTMLI 427

Query: 475 AGILEKVRRDSALENKSFVSPLHVSVLIPEFVLSGLTEAFAAIAVMELLTTHLPESLRTV 534
           +GI+E+ RRD +L        + V  L P+ VL G  + F  + + E     +P ++R++
Sbjct: 428 SGIVERKRRDLSLNGVK----MSVFWLTPQQVLMGFYQVFTIVGLTEFFNKQVPINMRSI 487

Query: 535 AGAIFFLSLSLASYLSSVIVGILSKISKK--TPWLGGNDLNKNRLDYFFYVVAVIAGLNF 594
             ++ +L LSLASYLSS +V I+  ++ +    WL  +D++K++LD F+Y +A ++ LNF
Sbjct: 488 GNSLLYLGLSLASYLSSAMVSIVHSVTARGGQSWL-TDDIDKSKLDCFYYFIAALSTLNF 546

Query: 595 FYFRLVAAKF 597
            +F   A ++
Sbjct: 548 IFFFWCARRY 546