Spg012426 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg012426
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionAAA-ATPase
Locationscaffold1: 5780935 .. 5783342 (-)
RNA-Seq ExpressionSpg012426
SyntenySpg012426
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GGTTAATATACAACTTTAGCCCTTATATTTGAACCTCCTAACCTAATATTATAACCAGGTCTAGTTCAGATCCTTTTTATTTTAATCTACTAATATAATAACCTTTGTCCACATGGACAAATTTTATTTTAATCCACTAATATTATAACATTTGTCCAAATCGAAAATTTTAATCAAGTCTACCTACCTAGAAAAAAAAAACAAAAACAAAAATATATTATCCAAATCTTTCATTAAAGCTTACTGATTCTCTATTTCTGTCTACTATTTCGATGAAGAATAAAAATAATATATCTTCGACCAAAGCTTCCTGATTCTCTCTACAGGTTTAATGAAGAATAATTTTTTTAAAAAAAATCCAACATCAAATAAAACCATAATATTATTATAATGCTAACCATTGAAGCAGATTCCTAGCAACATCGTCAATCTTGGTGCTTAAGCTTTCAGACAGTAAGCCAGTTGTATAAATTCTTCCGTTCATCCGTATTCCGTCGCAACCCTGCTGAGTTTCATCGCTTGATTTCGCTACAATGTTTGCTTTCAAAGAAATGCCTCAGTCGGCGTCTTCCCTGTTCGCGGCCTATGCCTCCTTCGCCACTTCCATGATGATGATCCGTTCAATGACCAACGACCTTCTTCCTCCTCAACTCATCTCCTTCATCTCCTCCATTTTCTCCTACTTTTTTCCTCCTAAATCCTCTCCTCAGACCACCCTCGTCATCGAGAAGAAGACCAACTACACCAAAAACCAGGTCTATGAAGCTGCAGAGATTTACCTCCGTACCAAAATCAGCCCCTCAATGGACCGTCTCAAGGTCTCCAAAACTCCAAGGCAACAGAAAGTTACCCTCTCCATGGAAAAGGGTCAAGAAATCGTTGATGAATTTCAAGACATTCAGCTCCGATGGCGATTCGTCTCCGAGAAAAAGCAGAAAGATGATGGATACAGCAAGGAGAAGCGCCATTACGAGCTTGTTTTCGATAAGAAATTCATGGATGAAGTCGTCGATTTATATTTTCCTTACATCTTACGGAGAGCCAAGGAGATTAAGGAGATGGAGAATGTTGCGAAACTCTGTAGCCAAAACTGTTCGTTCAATGACGATTCTGGTGACGATGGATGTCGAGGGAATTGGGGATCCATCAGTCTGGAGCATCCGGCTACGTTTGATACTCTGGCGATGGACCCTGATTTGAAGAAGATGATACTCGACGATTTGGATAGATTCGTCAAGAGGAAGGAATTCTATCGGAAGGTCGGGAAGGCTTGGAAGCGAGGCTATTTGTTGTACGGTCCGCCTGGTACAGGGAAATCCAGCTTAATCGCCGCCATGGCTAATTACCTCAAATTTGATATCTACGATTTAGATCTCACGGATATCGACAGCAACAACGAACTTAGAAGAGCGTTACTCTCCACTACAAATCGCTCAATTTTGGTGATTGAGGATATAGATTGCAGCGTGAATTTGCAGAATCGGGAAAAAGGCGACGAGGACAACGAGAATCTCGAACCTCCGAGAAGCAGGGTAGGGTTTCCCCCATTTTCCCCATTTCGAATTGTTGGAATCTACTCTAAATAATCGAAATTGAAATTGAAATTTGTGTGCAGTTGACGCTGTCAGGTATGCTCAATTTCATGGATGGATTGTGGTCGAGTTGCGGCGATGAGAGGATCATCGTTCTGACGACAAATCACAAGGAGCGATTGGACCCGGCGCTGTTGCGACCTGGTCGAATGGATGTGCACATAAACTTGAGCTACTGCACATCAAAGGCCTTCGAGGTTTTGGCCACCAATTACCTCGGCGGCGGAGCGATTCGTCACCCGCTGTACGAAGAAATCGAAGGGGTCATCGATTACGCCAACGTGACTCCGGCGGAGGTAGCGGAGGAGTTGATGAAGGGCGACGACGTTAACATGGTGATGGAAGGTTTAGCCAAGTTGGTGAAGGTAAAGATAGAGGAAAAGAGTAAGGTGAGTGACGGAAATGAGGCGCCGGAGGAAGAGGGCGAAGGGATAGTGGAAGAAGGGAAAGAAGAAAAAGTGAAGAGAAGAAAAAGCGGCAGCGGAGTTAGAAGGTACGGAAGAGGACTTAGAATTAGAAACAGAGGTCGTCGGTCGCCGTCGTCGTCGGTGGTCTACTAAAACAATCGTGGCTGAAGAAACTAAAGAGGAAGTTGGAAAGCGAATTAGGTAAGAGCCACGTGGGAGCCTCTTTTTCAGGAATAAAAATTCAGCAATCAGCTTTTGAACAACTATTTTCTATTCCGAAGAGTTTTTCCCCTCTCATTTTCACAGGTTAAATTATTAAATATACCCCAATAAGTTTCAATTATATCCCTAGAGTTTTAAAAGTTTCAATTATATCTTTGGAGTTTCAAGTTTGTTTCAAAATTGTCCT

mRNA sequence

ATGTTTGCTTTCAAAGAAATGCCTCAGTCGGCGTCTTCCCTGTTCGCGGCCTATGCCTCCTTCGCCACTTCCATGATGATGATCCGTTCAATGACCAACGACCTTCTTCCTCCTCAACTCATCTCCTTCATCTCCTCCATTTTCTCCTACTTTTTTCCTCCTAAATCCTCTCCTCAGACCACCCTCGTCATCGAGAAGAAGACCAACTACACCAAAAACCAGGTCTATGAAGCTGCAGAGATTTACCTCCGTACCAAAATCAGCCCCTCAATGGACCGTCTCAAGGTCTCCAAAACTCCAAGGCAACAGAAAGTTACCCTCTCCATGGAAAAGGGTCAAGAAATCGTTGATGAATTTCAAGACATTCAGCTCCGATGGCGATTCGTCTCCGAGAAAAAGCAGAAAGATGATGGATACAGCAAGGAGAAGCGCCATTACGAGCTTGTTTTCGATAAGAAATTCATGGATGAAGTCGTCGATTTATATTTTCCTTACATCTTACGGAGAGCCAAGGAGATTAAGGAGATGGAGAATGTTGCGAAACTCTGTAGCCAAAACTGTTCGTTCAATGACGATTCTGGTGACGATGGATGTCGAGGGAATTGGGGATCCATCAGTCTGGAGCATCCGGCTACGTTTGATACTCTGGCGATGGACCCTGATTTGAAGAAGATGATACTCGACGATTTGGATAGATTCGTCAAGAGGAAGGAATTCTATCGGAAGGTCGGGAAGGCTTGGAAGCGAGGCTATTTGTTGTACGGTCCGCCTGGTACAGGGAAATCCAGCTTAATCGCCGCCATGGCTAATTACCTCAAATTTGATATCTACGATTTAGATCTCACGGATATCGACAGCAACAACGAACTTAGAAGAGCGTTACTCTCCACTACAAATCGCTCAATTTTGGTGATTGAGGATATAGATTGCAGCGTGAATTTGCAGAATCGGGAAAAAGGCGACGAGGACAACGAGAATCTCGAACCTCCGAGAAGCAGGTTGACGCTGTCAGGTATGCTCAATTTCATGGATGGATTGTGGTCGAGTTGCGGCGATGAGAGGATCATCGTTCTGACGACAAATCACAAGGAGCGATTGGACCCGGCGCTGTTGCGACCTGGTCGAATGGATGTGCACATAAACTTGAGCTACTGCACATCAAAGGCCTTCGAGGTTTTGGCCACCAATTACCTCGGCGGCGGAGCGATTCGTCACCCGCTGTACGAAGAAATCGAAGGGGTCATCGATTACGCCAACGTGACTCCGGCGGAGGTAGCGGAGGAGTTGATGAAGGGCGACGACGTTAACATGGTGATGGAAGGTTTAGCCAAGTTGGTGAAGGTAAAGATAGAGGAAAAGAGTAAGGTGAGTGACGGAAATGAGGCGCCGGAGGAAGAGGGCGAAGGGATAGTGGAAGAAGGGAAAGAAGAAAAAGTGAAGAGAAGAAAAAGCGGCAGCGGAGTTAGAAGGTACGGAAGAGGACTTAGAATTAGAAACAGAGGTCGTCGGTCGCCGTCGTCGTCGGTGGTCTACTAA

Coding sequence (CDS)

ATGTTTGCTTTCAAAGAAATGCCTCAGTCGGCGTCTTCCCTGTTCGCGGCCTATGCCTCCTTCGCCACTTCCATGATGATGATCCGTTCAATGACCAACGACCTTCTTCCTCCTCAACTCATCTCCTTCATCTCCTCCATTTTCTCCTACTTTTTTCCTCCTAAATCCTCTCCTCAGACCACCCTCGTCATCGAGAAGAAGACCAACTACACCAAAAACCAGGTCTATGAAGCTGCAGAGATTTACCTCCGTACCAAAATCAGCCCCTCAATGGACCGTCTCAAGGTCTCCAAAACTCCAAGGCAACAGAAAGTTACCCTCTCCATGGAAAAGGGTCAAGAAATCGTTGATGAATTTCAAGACATTCAGCTCCGATGGCGATTCGTCTCCGAGAAAAAGCAGAAAGATGATGGATACAGCAAGGAGAAGCGCCATTACGAGCTTGTTTTCGATAAGAAATTCATGGATGAAGTCGTCGATTTATATTTTCCTTACATCTTACGGAGAGCCAAGGAGATTAAGGAGATGGAGAATGTTGCGAAACTCTGTAGCCAAAACTGTTCGTTCAATGACGATTCTGGTGACGATGGATGTCGAGGGAATTGGGGATCCATCAGTCTGGAGCATCCGGCTACGTTTGATACTCTGGCGATGGACCCTGATTTGAAGAAGATGATACTCGACGATTTGGATAGATTCGTCAAGAGGAAGGAATTCTATCGGAAGGTCGGGAAGGCTTGGAAGCGAGGCTATTTGTTGTACGGTCCGCCTGGTACAGGGAAATCCAGCTTAATCGCCGCCATGGCTAATTACCTCAAATTTGATATCTACGATTTAGATCTCACGGATATCGACAGCAACAACGAACTTAGAAGAGCGTTACTCTCCACTACAAATCGCTCAATTTTGGTGATTGAGGATATAGATTGCAGCGTGAATTTGCAGAATCGGGAAAAAGGCGACGAGGACAACGAGAATCTCGAACCTCCGAGAAGCAGGTTGACGCTGTCAGGTATGCTCAATTTCATGGATGGATTGTGGTCGAGTTGCGGCGATGAGAGGATCATCGTTCTGACGACAAATCACAAGGAGCGATTGGACCCGGCGCTGTTGCGACCTGGTCGAATGGATGTGCACATAAACTTGAGCTACTGCACATCAAAGGCCTTCGAGGTTTTGGCCACCAATTACCTCGGCGGCGGAGCGATTCGTCACCCGCTGTACGAAGAAATCGAAGGGGTCATCGATTACGCCAACGTGACTCCGGCGGAGGTAGCGGAGGAGTTGATGAAGGGCGACGACGTTAACATGGTGATGGAAGGTTTAGCCAAGTTGGTGAAGGTAAAGATAGAGGAAAAGAGTAAGGTGAGTGACGGAAATGAGGCGCCGGAGGAAGAGGGCGAAGGGATAGTGGAAGAAGGGAAAGAAGAAAAAGTGAAGAGAAGAAAAAGCGGCAGCGGAGTTAGAAGGTACGGAAGAGGACTTAGAATTAGAAACAGAGGTCGTCGGTCGCCGTCGTCGTCGGTGGTCTACTAA

Protein sequence

MFAFKEMPQSASSLFAAYASFATSMMMIRSMTNDLLPPQLISFISSIFSYFFPPKSSPQTTLVIEKKTNYTKNQVYEAAEIYLRTKISPSMDRLKVSKTPRQQKVTLSMEKGQEIVDEFQDIQLRWRFVSEKKQKDDGYSKEKRHYELVFDKKFMDEVVDLYFPYILRRAKEIKEMENVAKLCSQNCSFNDDSGDDGCRGNWGSISLEHPATFDTLAMDPDLKKMILDDLDRFVKRKEFYRKVGKAWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLTDIDSNNELRRALLSTTNRSILVIEDIDCSVNLQNREKGDEDNENLEPPRSRLTLSGMLNFMDGLWSSCGDERIIVLTTNHKERLDPALLRPGRMDVHINLSYCTSKAFEVLATNYLGGGAIRHPLYEEIEGVIDYANVTPAEVAEELMKGDDVNMVMEGLAKLVKVKIEEKSKVSDGNEAPEEEGEGIVEEGKEEKVKRRKSGSGVRRYGRGLRIRNRGRRSPSSSVVY
Homology
BLAST of Spg012426 vs. NCBI nr
Match: XP_022132940.1 (AAA-ATPase At2g18193-like [Momordica charantia])

HSP 1 Score: 805.8 bits (2080), Expect = 2.1e-229
Identity = 421/511 (82.39%), Postives = 466/511 (91.19%), Query Frame = 0

Query: 1   MFAFKEMPQSASSLFAAYASFATSMMMIRSMTNDLLPPQLISFISSIFSYFFPPKSSPQT 60
           MFAFKEMPQSASSLFAAYASFATSMMMIRS+TNDLLPPQL+SFISSI  YFFPPKSSPQ 
Sbjct: 1   MFAFKEMPQSASSLFAAYASFATSMMMIRSITNDLLPPQLLSFISSILFYFFPPKSSPQA 60

Query: 61  TLVIEKKTNYTKNQVYEAAEIYLRTKISPSMDRLKVSKTPRQQKVTLSMEKGQEIVDEFQ 120
           TLVIEKK+N++KNQVYEAAEIYLRTKIS SMDRLKVSKT RQ+KV+LSMEKGQEI D+F+
Sbjct: 61  TLVIEKKSNFSKNQVYEAAEIYLRTKISSSMDRLKVSKTGRQKKVSLSMEKGQEITDDFE 120

Query: 121 DIQLRWRFVSEKKQKDDGYSKEKRHYELVFDKKFMDEVVDLYFPYILRRAKEIKEMENVA 180
           +I L WRFVS +K++D  Y+KEK HYELVFDKKF D ++D YFPYILRRAKEIKE++NVA
Sbjct: 121 NIHLTWRFVSVEKKEDGEYAKEKYHYELVFDKKFTDTIIDFYFPYILRRAKEIKELDNVA 180

Query: 181 KLCSQNCSFNDDS-GDDGCRGNWGSISLEHPATFDTLAMDPDLKKMILDDLDRFVKRKEF 240
           KLCSQ+C+++DDS G DGCRGNWGSI+LEHPATFDTLA+DPDLKKMI+DDLDRFVKRKEF
Sbjct: 181 KLCSQDCTYDDDSGGSDGCRGNWGSIALEHPATFDTLAVDPDLKKMIIDDLDRFVKRKEF 240

Query: 241 YRKVGKAWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLTDIDSNNELRRALLSTTN 300
           YRKVGKAWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLTDIDSN  LRRALLSTTN
Sbjct: 241 YRKVGKAWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLTDIDSNYNLRRALLSTTN 300

Query: 301 RSILVIEDIDCSVNLQNREKGDEDNENLEPPRSRLTLSGMLNFMDGLWSSCGDERIIVLT 360
           RSILVIEDIDCSVNLQNRE  DE+N+  +  RSRLTLSGMLNF+DGLWSSCGDERIIVLT
Sbjct: 301 RSILVIEDIDCSVNLQNRE-SDENND--DSTRSRLTLSGMLNFIDGLWSSCGDERIIVLT 360

Query: 361 TNHKERLDPALLRPGRMDVHINLSYCTSKAFEVLATNYLGGGAIRHPLYEEIEGVIDYAN 420
           TNHKERLDPALLRPGRMDVHINLSYCTSKAF+VLATNYLGG A  HPLYEEIEG+ID AN
Sbjct: 361 TNHKERLDPALLRPGRMDVHINLSYCTSKAFKVLATNYLGGEATAHPLYEEIEGLIDGAN 420

Query: 421 VTPAEVAEELMKGDDVNMVMEGLAKLVKVKIEEKSKVSDGNEAP-EEEGEGIVEEGKEEK 480
           VTPAEVAEELMK +D++ VMEGLAK V++K EE+   S GNEAP EEEG+ IVEE +EEK
Sbjct: 421 VTPAEVAEELMKSNDIDTVMEGLAKFVRLKREEQ---SGGNEAPEEEEGDEIVEEEQEEK 480

Query: 481 VKRRKSGSGVRRYGRGLRIRNRGRRSPSSSV 510
           + R+K+G+GVRRY RGLR+ +R RR PSSSV
Sbjct: 481 LNRKKNGAGVRRYRRGLRMGSRSRRPPSSSV 505

BLAST of Spg012426 vs. NCBI nr
Match: XP_023517746.1 (AAA-ATPase At2g18193-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 780.4 bits (2014), Expect = 9.5e-222
Identity = 394/473 (83.30%), Postives = 438/473 (92.60%), Query Frame = 0

Query: 1   MFAFKEMPQSASSLFAAYASFATSMMMIRSMTNDLLPPQLISFISSIFSYFFPPKSSPQT 60
           MF+FK+MP SASSLFAAYASFATSMMMIRS+TNDLLPPQLISFISSIF+YFFPPKSSPQT
Sbjct: 1   MFSFKDMPPSASSLFAAYASFATSMMMIRSITNDLLPPQLISFISSIFTYFFPPKSSPQT 60

Query: 61  TLVIEKKTNYTKNQVYEAAEIYLRTKISPSMDRLKVSKTPRQQKVTLSMEKGQEIVDEFQ 120
           TLVIEKKTNY KNQV+EAAEIYLRTKISPSMDRLKVSKTPRQQ+V+LSMEK QEIVD+F+
Sbjct: 61  TLVIEKKTNYNKNQVFEAAEIYLRTKISPSMDRLKVSKTPRQQRVSLSMEKDQEIVDQFE 120

Query: 121 DIQLRWRFVSEKKQKDDGYSKEKRHYELVFDKKFMDEVVDLYFPYILRRAKEIKEMENVA 180
           DI L+WRFV+EKK++ +   KEKRHYELVFDKKFMD+VVD Y PYIL+RAKEI EMENV+
Sbjct: 121 DIHLKWRFVAEKKKEGNESVKEKRHYELVFDKKFMDKVVDFYLPYILQRAKEITEMENVS 180

Query: 181 KLCSQNCSFNDDSGDDGCRGNWGSISLEHPATFDTLAMDPDLKKMILDDLDRFVKRKEFY 240
           KLCSQN S++DD GD+ CRGNWGSI LEHPATFDTLAMDPDLKKMI+DDLDRF+KRKEFY
Sbjct: 181 KLCSQNLSYSDDFGDERCRGNWGSIGLEHPATFDTLAMDPDLKKMIIDDLDRFLKRKEFY 240

Query: 241 RKVGKAWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLTDIDSNNELRRALLSTTNR 300
           RKVGKAWKRGYLLYGPPGTGKSSL+AAMANYLKFDIYDLDLTDI SN++LRR+LLST+NR
Sbjct: 241 RKVGKAWKRGYLLYGPPGTGKSSLVAAMANYLKFDIYDLDLTDICSNSDLRRSLLSTSNR 300

Query: 301 SILVIEDIDCSVNLQNREKGDEDNENLEPPRSRLTLSGMLNFMDGLWSSCGDERIIVLTT 360
           SILVIEDIDCSVNLQNR   D+++EN +  RS+LTLSGMLNFMDGLWSSCGDERIIVLTT
Sbjct: 301 SILVIEDIDCSVNLQNRANSDDESENNDDYRSKLTLSGMLNFMDGLWSSCGDERIIVLTT 360

Query: 361 NHKERLDPALLRPGRMDVHINLSYCTSKAFEVLATNYLGGGAIRHPLYEEIEGVIDYANV 420
           NHK+RLDPALLRPGRMDVHI+LSYCTSKAFE LATNYLGGGAI HPLYEEIE ++++ANV
Sbjct: 361 NHKDRLDPALLRPGRMDVHIHLSYCTSKAFEALATNYLGGGAIHHPLYEEIEALVEHANV 420

Query: 421 TPAEVAEELMKGDDVNMVMEGLAKLVKVKIEEKSKVSDGNEAPEEEGEGIVEE 474
           TPAEVAEELMKGDD+++VMEGLAK VK K EE+   +D N+A EEEG  IVEE
Sbjct: 421 TPAEVAEELMKGDDIDIVMEGLAKFVKRKREEQ---NDRNKAAEEEGNEIVEE 470

BLAST of Spg012426 vs. NCBI nr
Match: XP_022962680.1 (AAA-ATPase At2g18193-like [Cucurbita moschata])

HSP 1 Score: 780.0 bits (2013), Expect = 1.2e-221
Identity = 395/473 (83.51%), Postives = 435/473 (91.97%), Query Frame = 0

Query: 1   MFAFKEMPQSASSLFAAYASFATSMMMIRSMTNDLLPPQLISFISSIFSYFFPPKSSPQT 60
           M  FKEMP SASSLFAAYASFATSMMMIRS+TNDLLPPQLISFISSIF+YFFPPKSSPQT
Sbjct: 1   MLNFKEMPPSASSLFAAYASFATSMMMIRSITNDLLPPQLISFISSIFTYFFPPKSSPQT 60

Query: 61  TLVIEKKTNYTKNQVYEAAEIYLRTKISPSMDRLKVSKTPRQQKVTLSMEKGQEIVDEFQ 120
           TLVIEKKTNY KNQV+EAAEIYLRTKISPSMDRLKVSKTPRQQ+V+LSMEK QEIVD+F+
Sbjct: 61  TLVIEKKTNYNKNQVFEAAEIYLRTKISPSMDRLKVSKTPRQQRVSLSMEKDQEIVDQFE 120

Query: 121 DIQLRWRFVSEKKQKDDGYSKEKRHYELVFDKKFMDEVVDLYFPYILRRAKEIKEMENVA 180
           DI L+WRFV+EKK++ + + KEKRHYELVFDKKFMD+VVD Y PYILRRAKEI EMENV+
Sbjct: 121 DIHLKWRFVAEKKKEGNEFVKEKRHYELVFDKKFMDKVVDFYLPYILRRAKEITEMENVS 180

Query: 181 KLCSQNCSFNDDSGDDGCRGNWGSISLEHPATFDTLAMDPDLKKMILDDLDRFVKRKEFY 240
           KLCSQN S++DD GD+ CRGNWGSI LEHPATFDTLAMDPDLKKMI+DDLDRF+KRKEFY
Sbjct: 181 KLCSQNLSYSDDFGDERCRGNWGSIGLEHPATFDTLAMDPDLKKMIIDDLDRFLKRKEFY 240

Query: 241 RKVGKAWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLTDIDSNNELRRALLSTTNR 300
           RKVGKAWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLTDI SN+ELRR+LLST+NR
Sbjct: 241 RKVGKAWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLTDICSNSELRRSLLSTSNR 300

Query: 301 SILVIEDIDCSVNLQNREKGDEDNENLEPPRSRLTLSGMLNFMDGLWSSCGDERIIVLTT 360
           SILVIEDIDCSVNLQNR   D+++EN +  RS+LTLSGMLNFMDGLWSSCGDERIIVLTT
Sbjct: 301 SILVIEDIDCSVNLQNRANSDDESENNDDYRSKLTLSGMLNFMDGLWSSCGDERIIVLTT 360

Query: 361 NHKERLDPALLRPGRMDVHINLSYCTSKAFEVLATNYLGGGAIRHPLYEEIEGVIDYANV 420
           NHK+RLDPALLRPGRMDVHI+LSYC+SKAFE L TNYLGGGAI HPLYEEIE ++++ANV
Sbjct: 361 NHKDRLDPALLRPGRMDVHIHLSYCSSKAFEALTTNYLGGGAIHHPLYEEIEALVEHANV 420

Query: 421 TPAEVAEELMKGDDVNMVMEGLAKLVKVKIEEKSKVSDGNEAPEEEGEGIVEE 474
           TPAEVAEELMK DD+++VMEGLAK VK K EE+   +DGN A EEEG  IVEE
Sbjct: 421 TPAEVAEELMKDDDIDIVMEGLAKFVKRKREEQ---NDGNNAAEEEGNEIVEE 470

BLAST of Spg012426 vs. NCBI nr
Match: KAG6594808.1 (Phosphatidylinositol/phosphatidylcholine transfer protein SFH12, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 766.5 bits (1978), Expect = 1.4e-217
Identity = 389/472 (82.42%), Postives = 433/472 (91.74%), Query Frame = 0

Query: 7   MPQSASSLFAAYASFATSMMMIRSMTNDLLPPQLISFISSIFSYFFPPKSSPQTTLVIEK 66
           MP SASSLFAAYASFATSMMMIRS+TNDLLPPQLISFISSIF+YFFPPKSSPQTTLVIEK
Sbjct: 1   MPPSASSLFAAYASFATSMMMIRSITNDLLPPQLISFISSIFTYFFPPKSSPQTTLVIEK 60

Query: 67  KTNYTKNQVYEAAEIYLRTKISPSMDRLKVSKTPRQQKVTLSMEKGQEIVDEFQDIQLRW 126
           KTNY KNQV+EAAEIYLRTKISPSMDRLKVSKTPRQQ+V+LSMEK QEIVD+F+DI L+W
Sbjct: 61  KTNYNKNQVFEAAEIYLRTKISPSMDRLKVSKTPRQQRVSLSMEKDQEIVDQFEDIHLKW 120

Query: 127 RFVSEKKQKDDGYSKEKRHYELVFDKKFMDEVVDLYFPYILRRAKEIKEMENVAKLCSQN 186
           RFV+EKK++ + + KEKRHYELVFDKKFMD+VVD Y PYILRRAKEI EMENV+KLCSQN
Sbjct: 121 RFVAEKKKEGNEFVKEKRHYELVFDKKFMDKVVDFYLPYILRRAKEITEMENVSKLCSQN 180

Query: 187 CSFNDDSGDDGCRGNWGSISLEHPATFDTLAMDPDLKKMILDDLDRFVKRKEFYRKVGKA 246
            S++DD GD+ CRGNWGSI LEHPATFDTLAMDPDLKKMI+DDLDRF+KRKEFYRKVGKA
Sbjct: 181 LSYSDDFGDERCRGNWGSIGLEHPATFDTLAMDPDLKKMIIDDLDRFLKRKEFYRKVGKA 240

Query: 247 WKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLTDIDSNNELRRALLSTTNRSILVIE 306
           WKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLTDI SN+ELRR+LLST+NRSILVIE
Sbjct: 241 WKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLTDICSNSELRRSLLSTSNRSILVIE 300

Query: 307 DIDCSVNLQNREKGDEDNENLEPPRSRLTLSGMLNFMDGLWSSCGDERIIVLTTNHKERL 366
           DIDCSVNLQNR   D+++EN +  RS+LTLSGMLNFMDGLWSSCGDERIIVLTTNHK+RL
Sbjct: 301 DIDCSVNLQNRANSDDESENNDDYRSKLTLSGMLNFMDGLWSSCGDERIIVLTTNHKDRL 360

Query: 367 DPALLRPGRMDVHINLSYCTSKAFEVLATNYLGGGAIRHPLYEEIEGVIDYANVTPAEVA 426
           DPALLRPGRMDVHI+LSYC+SKAFE L TNYLGGGAI HPLYEEIE ++++ANVTPAEVA
Sbjct: 361 DPALLRPGRMDVHIHLSYCSSKAFEALTTNYLGGGAIHHPLYEEIEALVEHANVTPAEVA 420

Query: 427 EELMKGDDVNMVMEGLAKLVKVKIEEKSKVSDGNEAPEEEGEGIVEEGKEEK 479
           EELMK DD+++VMEGLAK VK K EE+   +DGN A EEEG  + E  ++E+
Sbjct: 421 EELMKDDDIDIVMEGLAKFVKRKREEQ---NDGNNAAEEEGSDL-ESSEDER 468

BLAST of Spg012426 vs. NCBI nr
Match: XP_022962730.1 (AAA-ATPase At2g18193-like [Cucurbita moschata])

HSP 1 Score: 748.4 bits (1931), Expect = 4.0e-212
Identity = 383/492 (77.85%), Postives = 434/492 (88.21%), Query Frame = 0

Query: 1   MFAFKEMPQSASSLFAAYASFATSMMMIRSMTNDLLPPQLISFISSIFSYFFPPKSSPQT 60
           MF+FK+MP SA+SLFAAYASFATSMMMIRS+TNDLLPP+LISFISSIF+YFF  KSSPQT
Sbjct: 1   MFSFKDMPPSAASLFAAYASFATSMMMIRSITNDLLPPKLISFISSIFNYFFTLKSSPQT 60

Query: 61  TLVIEKKTNYTKNQVYEAAEIYLRTKISPSMDRLKVSKTPRQQKVTLSMEKGQEIVDEFQ 120
           TLVI+ KTNY KNQV++AAEIYLRTKISPSMDRL  SKTPRQ +V+LSM+K QEIVD+FQ
Sbjct: 61  TLVIKNKTNYAKNQVFDAAEIYLRTKISPSMDRLAASKTPRQHRVSLSMDKDQEIVDQFQ 120

Query: 121 DIQLRWRFVSEKKQKDDGYSKEKRHYELVFDKKFMDEVVDLYFPYILRRAKEIKEMENVA 180
           DI L+WRFV+EK ++D  + +EKRHYEL F KKFMD+VVD Y PYILRRAKEIK MENV+
Sbjct: 121 DIHLKWRFVAEKTKEDRQHIEEKRHYELSFHKKFMDKVVDFYLPYILRRAKEIKAMENVS 180

Query: 181 KLCSQNCSFNDDSGDDGCRGNWGSISLEHPATFDTLAMDPDLKKMILDDLDRFVKRKEFY 240
           KLCSQN S++DD GD+ CRGNWGSI LEHPATFDTLAMDPDLKKMI+DDLDRF+KRKEFY
Sbjct: 181 KLCSQNLSYSDDFGDERCRGNWGSIGLEHPATFDTLAMDPDLKKMIIDDLDRFLKRKEFY 240

Query: 241 RKVGKAWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLTDIDSNNELRRALLSTTNR 300
           RKVGKAWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLTDI SN+ELRR+LLST+NR
Sbjct: 241 RKVGKAWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLTDICSNSELRRSLLSTSNR 300

Query: 301 SILVIEDIDCSVNLQNREKGDEDNENLEPPRSRLTLSGMLNFMDGLWSSCGDERIIVLTT 360
           SILVIEDIDCSVNLQNR   D+++EN +  RS+LTLSGMLNFMDGLWSSCGDERIIVLTT
Sbjct: 301 SILVIEDIDCSVNLQNRANSDDESENNDDYRSKLTLSGMLNFMDGLWSSCGDERIIVLTT 360

Query: 361 NHKERLDPALLRPGRMDVHINLSYCTSKAFEVLATNYLGGGAIRHPLYEEIEGVIDYANV 420
           NHK+RLDPALLRPGRMDVHI LSYCTSKAFE L TNYLGGGAI HPLYEEI+ +I+YANV
Sbjct: 361 NHKDRLDPALLRPGRMDVHIYLSYCTSKAFEALTTNYLGGGAIHHPLYEEIKALIEYANV 420

Query: 421 TPAEVAEELMKGDDVNMVMEGLAKLVKVKIEEKSKVSDGNEAPEEEGEGIVEEGKEEKVK 480
           TPAEVAEELMK DD+++VMEGLAK VK+K EE+   + GN+APE+EG  +   G     +
Sbjct: 421 TPAEVAEELMKDDDIDIVMEGLAKFVKLKREEQ---NGGNKAPEKEGNEMEVVGG----R 480

Query: 481 RRKSGSGVRRYG 493
           RR+     + +G
Sbjct: 481 RRRKWESTKNHG 485

BLAST of Spg012426 vs. ExPASy Swiss-Prot
Match: Q8GW96 (AAA-ATPase At2g18193 OS=Arabidopsis thaliana OX=3702 GN=At2g18193 PE=2 SV=1)

HSP 1 Score: 497.7 bits (1280), Expect = 1.6e-139
Identity = 261/495 (52.73%), Postives = 357/495 (72.12%), Query Frame = 0

Query: 1   MFAFKEMPQSASSLFAAYASFATSMMMIRSMTNDLLPPQLISFISSIFSYFFPPKSSPQT 60
           MF   +   S SSLF+AYAS    +M+ RSM +D +P +L S+ SS+   FF PKS    
Sbjct: 1   MFPSSDFSFSPSSLFSAYASLTGFLMLFRSMLHDFVPEKLRSYFSSLLDRFFTPKSK-YL 60

Query: 61  TLVIEKKTNYTKNQVYEAAEIYLRTKISPSMDRLKVSKTPRQQKVTLSMEKGQEIVDEFQ 120
           T++I++     +NQV++AAE+YLR+KI P  +RL+V K P+Q+  T+S+E+G+EI+D F+
Sbjct: 61  TVIIDENFGLNRNQVFDAAEMYLRSKIGPETERLRVGKIPKQKHFTISIERGEEILDTFE 120

Query: 121 DIQLRWRFVSEKKQKDDGYSKEKRHYELVFDKKFMDEVVDLYFPYILRRAKEIKEMENVA 180
           + +++W +V  + +K D   K KR+YEL F+KK  D+V++ Y  +++  ++EIK    V 
Sbjct: 121 ESEVKWSYVQSENEKGD---KVKRYYELTFEKKLRDKVLNSYLTHVVAESEEIKRNLRVV 180

Query: 181 KLCSQNCSFNDDSGDDG-CRGNWGSISLEHPATFDTLAMDPDLKKMILDDLDRFVKRKEF 240
           KL S++   +DD  DDG   GNWG I+LEHP+TFDTLAMDP+ KK I+DDL+RF+KRKEF
Sbjct: 181 KLYSRDVYASDD--DDGMAGGNWGCINLEHPSTFDTLAMDPNAKKKIIDDLERFLKRKEF 240

Query: 241 YRKVGKAWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLTDIDSNNELRRALLSTTN 300
           Y++VGKAWKRGYLLYGPPGTGKSSLIAAMANYLKFD++DL+L+ I  N EL+R LLSTTN
Sbjct: 241 YKRVGKAWKRGYLLYGPPGTGKSSLIAAMANYLKFDVFDLELSSIYDNGELKRVLLSTTN 300

Query: 301 RSILVIEDIDCSVNLQNREKGDEDNENLEPPRSRLTLSGMLNFMDGLWSSCGDERIIVLT 360
           RSILVIEDIDC+  +++RE  ++++E +   + ++TLSG+LNF+DGLWSS GDERIIV T
Sbjct: 301 RSILVIEDIDCNAEVRDREAENQEDEQI---KGKVTLSGILNFIDGLWSSFGDERIIVFT 360

Query: 361 TNHKERLDPALLRPGRMDVHINLSYCTSKAFEVLATNYLGGGAIRHPLYEEIEGVIDYAN 420
           TNHKERLDPALLRPGRMDVHIN+SYCT   F  L +NYLG   + HPL EEIE ++D   
Sbjct: 361 TNHKERLDPALLRPGRMDVHINMSYCTGLGFRTLVSNYLGLDGLNHPLCEEIEALVDSTE 420

Query: 421 VTPAEVAEELMKGDDVNMVMEGLAKLVKVKIEEKSKVSDGNEAPEEEGEGIVEEGKEEKV 480
           VTPAE+AEELM+ DD ++V+ G+   V+ +  E+SK        +   +   + G    V
Sbjct: 421 VTPAELAEELMQDDDTDVVLRGVISFVEKRKVERSKTKKEVSICKATDDDEKQNGSLGCV 480

Query: 481 KRRKSGSGVRRYGRG 495
           K++K G   +  G+G
Sbjct: 481 KKKKKGGKQKGKGKG 486

BLAST of Spg012426 vs. ExPASy Swiss-Prot
Match: F4IQG2 (AAA-ATPase At2g18190 OS=Arabidopsis thaliana OX=3702 GN=At2g18190 PE=2 SV=1)

HSP 1 Score: 473.4 bits (1217), Expect = 3.2e-132
Identity = 256/483 (53.00%), Postives = 343/483 (71.01%), Query Frame = 0

Query: 10  SASSLFAAYASFATSMMMIRSMTNDLLPPQLISFISSIFSYFFPPKSSPQTTLVIEKKTN 69
           S SSLF AYAS    +M+ RS+ ND +P +L S+I+ + + FF PKS    T+VI++   
Sbjct: 11  SPSSLFTAYASLTGFLMLFRSLFNDEVPERLRSYITDLLNRFFTPKSK-NLTMVIDEIIG 70

Query: 70  YTKNQVYEAAEIYLRTKISPSMDRLKVSKTPRQQKVTLSMEKGQEIVDEFQDIQLRWRFV 129
           + +NQV++AAE+YLR KI P   RL+V K P+Q+  T+ +EKG+EI+D F++ +LRW +V
Sbjct: 71  FKRNQVFDAAEVYLRNKIGPETARLRVGKLPKQKHFTIYIEKGEEILDTFENSELRWTYV 130

Query: 130 SEKKQKDDGYSKEKRHYELVFDKKFMDEVVDLYFPYILRRAKEIKEMENVAKLCSQNCSF 189
              + +++   KEKR+YEL F+KK  D+V++ Y  +++  ++E K      KL S++   
Sbjct: 131 ---ESENEASQKEKRYYELTFEKKLRDKVMNSYLSHVVAESEETKRDLRAVKLYSRDVRA 190

Query: 190 NDDSGDDGCRG-NWGSISLEHPATFDTLAMDPDLKKMILDDLDRFVKRKEFYRKVGKAWK 249
           + D  DDG  G  WG I+LEHP+TF+TLAMDP  KK I+DD++RF+KR+EFY++VGKAWK
Sbjct: 191 SKD--DDGMAGAGWGCINLEHPSTFETLAMDPGAKKKIIDDMERFLKRREFYKRVGKAWK 250

Query: 250 RGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLTDIDSNNELRRALLSTTNRSILVIEDI 309
           RGYLLYGPPGTGKSSLIAAMANYLKFD++DL+L+ I  N +L+  LLSTTNRSILVIEDI
Sbjct: 251 RGYLLYGPPGTGKSSLIAAMANYLKFDVFDLELSSIYENAQLKSILLSTTNRSILVIEDI 310

Query: 310 DCSVNLQNREKGDEDNENLEPPRSRLTLSGMLNFMDGLWSSCGDERIIVLTTNHKERLDP 369
           DCS       + DE  E  E    R+TLSG+LNF+DGLWSS GDERIIV TTNHKERLDP
Sbjct: 311 DCSSAEVVDREADEYQEYEEGYYGRVTLSGLLNFVDGLWSSFGDERIIVFTTNHKERLDP 370

Query: 370 ALLRPGRMDVHINLSYCTSKAFEVLATNYLGGGAIRHPLYEEIEGVIDYANVTPAEVAEE 429
           ALLRPGRMD+HIN+SYCT   F  L +NYLG G + HPL EEIE +ID   VTPAE+AEE
Sbjct: 371 ALLRPGRMDMHINMSYCTGLGFRTLVSNYLGLGGLNHPLCEEIEALIDSTEVTPAELAEE 430

Query: 430 LMKGDDVNMVMEGLAKLV---KVKIEEKSKVSDGNEAPEEEGE---GIVEEGKEEKVKRR 486
           LM+ DD ++V+ G+   V   KV+I  K+K  +G+   + +G+    +      +K K++
Sbjct: 431 LMQEDDTDVVLRGVVSFVENRKVEI-SKTKELEGSTCRKLDGDDKHNVSSTNDLKKTKKK 486

BLAST of Spg012426 vs. ExPASy Swiss-Prot
Match: Q9FN75 (AAA-ATPase At5g17760 OS=Arabidopsis thaliana OX=3702 GN=At5g17760 PE=3 SV=1)

HSP 1 Score: 429.5 bits (1103), Expect = 5.4e-119
Identity = 241/496 (48.59%), Postives = 328/496 (66.13%), Query Frame = 0

Query: 1   MFAFKEMPQSASSLFAAYASFATSMMMIRSMTNDLLPPQLISFISSIFSYFFPPKSSPQT 60
           MF  K++P S +S+F AYAS A  MMMIRSM ++L+P  L  FI       F   SS   
Sbjct: 1   MFFSKDLP-SPTSVFTAYASMAGYMMMIRSMAHELIPAPLQDFIYRTLRSLFFRSSSSTL 60

Query: 61  TLVIEKKTNYTKNQVYEAAEIYLRTKISPSMDRLKVSKTPRQQKVTLSMEKGQEIVDEFQ 120
           TL I+       N++Y AA+ YL TKISP   RL++SK  + + V L +  G+ + D ++
Sbjct: 61  TLTIDDDNMGMNNEIYRAAQTYLSTKISPDAVRLRISKGHKDKHVNLYLSDGEIVNDVYE 120

Query: 121 DIQLRWRFVSEKKQK---------------------DDGYSKEKRHYELVFDKKFMDEVV 180
           D+QL WRFV++   K                     DDG S+   ++EL FDKK  D ++
Sbjct: 121 DVQLVWRFVTDGGDKKGGGGGVGGRGGGGGRRGGMDDDGKSE---YFELSFDKKHKDLIL 180

Query: 181 DLYFPYILRRAKEIKEMENVAKLCSQNCSFNDDSGDDGCRGNWGSISLEHPATFDTLAMD 240
           + Y PYI  +AKEI++   +  L S N               W S+ LEHP+TF+T+AM+
Sbjct: 181 NSYVPYIESKAKEIRDERRILMLHSLNSL------------RWESVILEHPSTFETMAME 240

Query: 241 PDLKKMILDDLDRFVKRKEFYRKVGKAWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDL 300
            DLK+ +++DLDRF++RKEFY++VGKAWKRGYLLYGPPGTGKSSL+AAMANYLKFD+YDL
Sbjct: 241 DDLKRDVIEDLDRFIRRKEFYKRVGKAWKRGYLLYGPPGTGKSSLVAAMANYLKFDVYDL 300

Query: 301 DLTDIDSNNELRRALLSTTNRSILVIEDIDCSVNLQNREKGDEDNENLEPPRSRLTLSGM 360
            L  +  +++LRR LL+T NRSILVIEDIDC+V+L NR +   + +N    +  LTLSG+
Sbjct: 301 QLASVMRDSDLRRLLLATRNRSILVIEDIDCAVDLPNRIEQPVEGKNRGESQGPLTLSGL 360

Query: 361 LNFMDGLWSSCGDERIIVLTTNHKERLDPALLRPGRMDVHINLSYCTSKAFEVLATNYLG 420
           LNF+DGLWSSCGDERII+ TTNHK+RLDPALLRPGRMD+HI + +C+ + F+ LA+NYLG
Sbjct: 361 LNFIDGLWSSCGDERIIIFTTNHKDRLDPALLRPGRMDMHIYMGHCSFQGFKTLASNYLG 420

Query: 421 --GGAIRHPLYEEIEGVIDYANVTPAEVAEELMKGDDVNMVMEGLAKLVKVKIEEKSKVS 474
               A+ H L+ EIE +ID   +TPA+VAEELMK +D ++ +EGL  +++ K+  KSK S
Sbjct: 421 LSDAAMPHRLFPEIERLIDGEVMTPAQVAEELMKSEDADVALEGLVNVLE-KMRLKSKES 479

BLAST of Spg012426 vs. ExPASy Swiss-Prot
Match: Q147F9 (AAA-ATPase At3g50940 OS=Arabidopsis thaliana OX=3702 GN=At3g50940 PE=2 SV=1)

HSP 1 Score: 414.5 bits (1064), Expect = 1.8e-114
Identity = 226/449 (50.33%), Postives = 305/449 (67.93%), Query Frame = 0

Query: 10  SASSLFAAYASFATSMMMIRSMTNDLLPPQLISFISSIFSYFFPPKSSPQTTLVIEKKTN 69
           +A +   A AS A + ++ RS+  D +P ++  +IS  F  FF    S Q T VIE+   
Sbjct: 11  TAKTALTAVASVAAAAILARSVVQDYMPNEVHEYISHGFRRFF-SYFSYQMTAVIEEFGG 70

Query: 70  YTKNQVYEAAEIYLRTKISPSMDRLKVSKTPRQQKVTLSMEKGQEIVDEFQDIQLRWRFV 129
           +  NQV+EAAE YL TKIS S  R+KV+K  +Q   ++++E+ +E+VD F  ++L W  V
Sbjct: 71  FEHNQVFEAAEAYLSTKISNSTRRIKVNKLEKQSNYSVTVERDEEVVDIFDGVKLSWILV 130

Query: 130 SEKKQKDD---------GYSKEKRHYELVFDKKFMDEVVDLYFPYILRRAKEIKEMENVA 189
                K D             E R YEL F KKF + V++ Y P+++ +A  IK+     
Sbjct: 131 CRHVDKKDFRNPRDLNSTLKSEVRSYELSFRKKFKNMVLESYLPFVVEQAASIKQKFKTL 190

Query: 190 KLCSQNCSFNDDSGDDGCRGNWGSISLEHPATFDTLAMDPDLKKMILDDLDRFVKRKEFY 249
           K+ +           D     W S++L+HP+TF TLA+DP++KK +++DLDRFV+RK FY
Sbjct: 191 KIFTV----------DSYSVEWTSVTLDHPSTFRTLALDPEVKKNLVEDLDRFVQRKGFY 250

Query: 250 RKVGKAWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLTDIDSNNELRRALLSTTNR 309
            +VGKAWKRGYLLYGPPGTGKSSLIAA+AN+L FDIYDLDLT +++N ELRR L+ST NR
Sbjct: 251 GRVGKAWKRGYLLYGPPGTGKSSLIAAIANHLNFDIYDLDLTSLNNNAELRRLLMSTANR 310

Query: 310 SILVIEDIDCSVNLQNREKGDEDNENLEPPRSRLTLSGMLNFMDGLWSSCGDERIIVLTT 369
           SILV+EDIDCS+ L++R     D EN +P    +TLSG+LNF+DGLWSSCG+ERIIV TT
Sbjct: 311 SILVVEDIDCSIELKDR---STDQENNDPLHKTVTLSGLLNFVDGLWSSCGNERIIVFTT 370

Query: 370 NHKERLDPALLRPGRMDVHINLSYCTSKAFEVLATNYLGGGAIRHPLYEEIEGVIDYANV 429
           N++E+LDPALLRPGRMD+HI++SYCT  AF+VLA+NYL      H L+E+IE  I    V
Sbjct: 371 NYREKLDPALLRPGRMDMHIHMSYCTPAAFKVLASNYL--EIQDHILFEQIEEFIREIEV 430

Query: 430 TPAEVAEELMKGDDVNMVMEGLAKLVKVK 450
           TPAEVAE+LM+ D V+ V++GL + +K K
Sbjct: 431 TPAEVAEQLMRSDSVDKVLQGLVEFLKAK 443

BLAST of Spg012426 vs. ExPASy Swiss-Prot
Match: Q8VZG2 (Protein HYPER-SENSITIVITY-RELATED 4 OS=Arabidopsis thaliana OX=3702 GN=HSR4 PE=2 SV=1)

HSP 1 Score: 412.9 bits (1060), Expect = 5.2e-114
Identity = 230/496 (46.37%), Postives = 332/496 (66.94%), Query Frame = 0

Query: 10  SASSLFAAYASFATSMMMIRSMTNDLLPPQLISFIS----SIFSYFFPPKSSPQTTLVIE 69
           +A ++    AS A + M+ RS+  D LP ++  +IS    SIF YF     S Q T++IE
Sbjct: 57  TAKTVLTTAASVAATAMLARSLVQDYLPDEVHHYISYGFRSIFGYF-----SSQMTIIIE 116

Query: 70  KKTNYTKNQVYEAAEIYLRTKISPSMDRLKVSKTPRQQKVTLSMEKGQEIVDEFQDIQLR 129
           +   +  N+V+EAAE YL TKISPS  R+KVSK  ++    +++E+ +E+VD +  ++ +
Sbjct: 117 EFEGFAHNEVFEAAEAYLATKISPSNKRIKVSKHEKENNYNVTVERDEEVVDTYNGVKFQ 176

Query: 130 W----RFVSEK-----KQKDDGYSKEKRHYELVFDKKFMDEVVDLYFPYILRRAKEIKEM 189
           W    R V  K     +  +     E R +EL F KKF D  ++ Y P++++RA  +K+ 
Sbjct: 177 WILHCRHVESKHFHNPRDLNSTLRSEVRSFELNFHKKFKDVALESYLPFMVKRATLMKQE 236

Query: 190 ENVAKLCSQNCSFNDDSGDDGCRGNWGSISLEHPATFDTLAMDPDLKKMILDDLDRFVKR 249
           +   K+ + +      +  D     W S++L+HP+TF TLAMD D+K  +++DLD+FVKR
Sbjct: 237 KKTLKIFTLSPENMYGNYSDA----WTSVTLDHPSTFKTLAMDSDVKTSVMEDLDKFVKR 296

Query: 250 KEFYRKVGKAWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLTDIDSNNELRRALLS 309
           ++FY++VGKAWKRGYLLYGPPGTGKSSLIAAMAN+L FDIYDL+LT +++N+ELRR L++
Sbjct: 297 RDFYKRVGKAWKRGYLLYGPPGTGKSSLIAAMANHLNFDIYDLELTAVNNNSELRRLLIA 356

Query: 310 TTNRSILVIEDIDCSVNLQNR--EKGDEDNENLEPPR-SRLTLSGMLNFMDGLWSSCGDE 369
           T NRSIL++EDIDCS+ L++R  ++   +++++E PR  ++TLSG+LNF+DGLWSSCGDE
Sbjct: 357 TANRSILIVEDIDCSLELKDRTSDEPPRESDDIEDPRYKKVTLSGLLNFIDGLWSSCGDE 416

Query: 370 RIIVLTTNHKERLDPALLRPGRMDVHINLSYCTSKAFEVLATNYLGGGAIRHPLYEEIEG 429
           RII+ TTN+KE+LD ALLRPGRMD+HI++SYCT   F+ LA NYL      H L+ +IE 
Sbjct: 417 RIIIFTTNYKEKLDAALLRPGRMDMHIHMSYCTPSTFKALALNYL--EIKEHRLFSKIEE 476

Query: 430 VIDYANVTPAEVAEELMKGDDVNMVMEGLAKLVKVKIEEKSKVSDGNEAPEEEGEGIVEE 489
            I+   VTPAEVAE+LM+ D V+ V+EGL + +KVK  E  +     E  E E +   +E
Sbjct: 477 GIEATEVTPAEVAEQLMRNDSVDKVLEGLIEFLKVKKIENEQDKAKTEKQELENKKKTKE 536

BLAST of Spg012426 vs. ExPASy TrEMBL
Match: A0A6J1BTY0 (AAA-ATPase At2g18193-like OS=Momordica charantia OX=3673 GN=LOC111005660 PE=3 SV=1)

HSP 1 Score: 805.8 bits (2080), Expect = 1.0e-229
Identity = 421/511 (82.39%), Postives = 466/511 (91.19%), Query Frame = 0

Query: 1   MFAFKEMPQSASSLFAAYASFATSMMMIRSMTNDLLPPQLISFISSIFSYFFPPKSSPQT 60
           MFAFKEMPQSASSLFAAYASFATSMMMIRS+TNDLLPPQL+SFISSI  YFFPPKSSPQ 
Sbjct: 1   MFAFKEMPQSASSLFAAYASFATSMMMIRSITNDLLPPQLLSFISSILFYFFPPKSSPQA 60

Query: 61  TLVIEKKTNYTKNQVYEAAEIYLRTKISPSMDRLKVSKTPRQQKVTLSMEKGQEIVDEFQ 120
           TLVIEKK+N++KNQVYEAAEIYLRTKIS SMDRLKVSKT RQ+KV+LSMEKGQEI D+F+
Sbjct: 61  TLVIEKKSNFSKNQVYEAAEIYLRTKISSSMDRLKVSKTGRQKKVSLSMEKGQEITDDFE 120

Query: 121 DIQLRWRFVSEKKQKDDGYSKEKRHYELVFDKKFMDEVVDLYFPYILRRAKEIKEMENVA 180
           +I L WRFVS +K++D  Y+KEK HYELVFDKKF D ++D YFPYILRRAKEIKE++NVA
Sbjct: 121 NIHLTWRFVSVEKKEDGEYAKEKYHYELVFDKKFTDTIIDFYFPYILRRAKEIKELDNVA 180

Query: 181 KLCSQNCSFNDDS-GDDGCRGNWGSISLEHPATFDTLAMDPDLKKMILDDLDRFVKRKEF 240
           KLCSQ+C+++DDS G DGCRGNWGSI+LEHPATFDTLA+DPDLKKMI+DDLDRFVKRKEF
Sbjct: 181 KLCSQDCTYDDDSGGSDGCRGNWGSIALEHPATFDTLAVDPDLKKMIIDDLDRFVKRKEF 240

Query: 241 YRKVGKAWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLTDIDSNNELRRALLSTTN 300
           YRKVGKAWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLTDIDSN  LRRALLSTTN
Sbjct: 241 YRKVGKAWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLTDIDSNYNLRRALLSTTN 300

Query: 301 RSILVIEDIDCSVNLQNREKGDEDNENLEPPRSRLTLSGMLNFMDGLWSSCGDERIIVLT 360
           RSILVIEDIDCSVNLQNRE  DE+N+  +  RSRLTLSGMLNF+DGLWSSCGDERIIVLT
Sbjct: 301 RSILVIEDIDCSVNLQNRE-SDENND--DSTRSRLTLSGMLNFIDGLWSSCGDERIIVLT 360

Query: 361 TNHKERLDPALLRPGRMDVHINLSYCTSKAFEVLATNYLGGGAIRHPLYEEIEGVIDYAN 420
           TNHKERLDPALLRPGRMDVHINLSYCTSKAF+VLATNYLGG A  HPLYEEIEG+ID AN
Sbjct: 361 TNHKERLDPALLRPGRMDVHINLSYCTSKAFKVLATNYLGGEATAHPLYEEIEGLIDGAN 420

Query: 421 VTPAEVAEELMKGDDVNMVMEGLAKLVKVKIEEKSKVSDGNEAP-EEEGEGIVEEGKEEK 480
           VTPAEVAEELMK +D++ VMEGLAK V++K EE+   S GNEAP EEEG+ IVEE +EEK
Sbjct: 421 VTPAEVAEELMKSNDIDTVMEGLAKFVRLKREEQ---SGGNEAPEEEEGDEIVEEEQEEK 480

Query: 481 VKRRKSGSGVRRYGRGLRIRNRGRRSPSSSV 510
           + R+K+G+GVRRY RGLR+ +R RR PSSSV
Sbjct: 481 LNRKKNGAGVRRYRRGLRMGSRSRRPPSSSV 505

BLAST of Spg012426 vs. ExPASy TrEMBL
Match: A0A6J1HFS7 (AAA-ATPase At2g18193-like OS=Cucurbita moschata OX=3662 GN=LOC111463099 PE=3 SV=1)

HSP 1 Score: 780.0 bits (2013), Expect = 6.0e-222
Identity = 395/473 (83.51%), Postives = 435/473 (91.97%), Query Frame = 0

Query: 1   MFAFKEMPQSASSLFAAYASFATSMMMIRSMTNDLLPPQLISFISSIFSYFFPPKSSPQT 60
           M  FKEMP SASSLFAAYASFATSMMMIRS+TNDLLPPQLISFISSIF+YFFPPKSSPQT
Sbjct: 1   MLNFKEMPPSASSLFAAYASFATSMMMIRSITNDLLPPQLISFISSIFTYFFPPKSSPQT 60

Query: 61  TLVIEKKTNYTKNQVYEAAEIYLRTKISPSMDRLKVSKTPRQQKVTLSMEKGQEIVDEFQ 120
           TLVIEKKTNY KNQV+EAAEIYLRTKISPSMDRLKVSKTPRQQ+V+LSMEK QEIVD+F+
Sbjct: 61  TLVIEKKTNYNKNQVFEAAEIYLRTKISPSMDRLKVSKTPRQQRVSLSMEKDQEIVDQFE 120

Query: 121 DIQLRWRFVSEKKQKDDGYSKEKRHYELVFDKKFMDEVVDLYFPYILRRAKEIKEMENVA 180
           DI L+WRFV+EKK++ + + KEKRHYELVFDKKFMD+VVD Y PYILRRAKEI EMENV+
Sbjct: 121 DIHLKWRFVAEKKKEGNEFVKEKRHYELVFDKKFMDKVVDFYLPYILRRAKEITEMENVS 180

Query: 181 KLCSQNCSFNDDSGDDGCRGNWGSISLEHPATFDTLAMDPDLKKMILDDLDRFVKRKEFY 240
           KLCSQN S++DD GD+ CRGNWGSI LEHPATFDTLAMDPDLKKMI+DDLDRF+KRKEFY
Sbjct: 181 KLCSQNLSYSDDFGDERCRGNWGSIGLEHPATFDTLAMDPDLKKMIIDDLDRFLKRKEFY 240

Query: 241 RKVGKAWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLTDIDSNNELRRALLSTTNR 300
           RKVGKAWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLTDI SN+ELRR+LLST+NR
Sbjct: 241 RKVGKAWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLTDICSNSELRRSLLSTSNR 300

Query: 301 SILVIEDIDCSVNLQNREKGDEDNENLEPPRSRLTLSGMLNFMDGLWSSCGDERIIVLTT 360
           SILVIEDIDCSVNLQNR   D+++EN +  RS+LTLSGMLNFMDGLWSSCGDERIIVLTT
Sbjct: 301 SILVIEDIDCSVNLQNRANSDDESENNDDYRSKLTLSGMLNFMDGLWSSCGDERIIVLTT 360

Query: 361 NHKERLDPALLRPGRMDVHINLSYCTSKAFEVLATNYLGGGAIRHPLYEEIEGVIDYANV 420
           NHK+RLDPALLRPGRMDVHI+LSYC+SKAFE L TNYLGGGAI HPLYEEIE ++++ANV
Sbjct: 361 NHKDRLDPALLRPGRMDVHIHLSYCSSKAFEALTTNYLGGGAIHHPLYEEIEALVEHANV 420

Query: 421 TPAEVAEELMKGDDVNMVMEGLAKLVKVKIEEKSKVSDGNEAPEEEGEGIVEE 474
           TPAEVAEELMK DD+++VMEGLAK VK K EE+   +DGN A EEEG  IVEE
Sbjct: 421 TPAEVAEELMKDDDIDIVMEGLAKFVKRKREEQ---NDGNNAAEEEGNEIVEE 470

BLAST of Spg012426 vs. ExPASy TrEMBL
Match: A0A6J1HFX6 (AAA-ATPase At2g18193-like OS=Cucurbita moschata OX=3662 GN=LOC111463134 PE=3 SV=1)

HSP 1 Score: 748.4 bits (1931), Expect = 1.9e-212
Identity = 383/492 (77.85%), Postives = 434/492 (88.21%), Query Frame = 0

Query: 1   MFAFKEMPQSASSLFAAYASFATSMMMIRSMTNDLLPPQLISFISSIFSYFFPPKSSPQT 60
           MF+FK+MP SA+SLFAAYASFATSMMMIRS+TNDLLPP+LISFISSIF+YFF  KSSPQT
Sbjct: 1   MFSFKDMPPSAASLFAAYASFATSMMMIRSITNDLLPPKLISFISSIFNYFFTLKSSPQT 60

Query: 61  TLVIEKKTNYTKNQVYEAAEIYLRTKISPSMDRLKVSKTPRQQKVTLSMEKGQEIVDEFQ 120
           TLVI+ KTNY KNQV++AAEIYLRTKISPSMDRL  SKTPRQ +V+LSM+K QEIVD+FQ
Sbjct: 61  TLVIKNKTNYAKNQVFDAAEIYLRTKISPSMDRLAASKTPRQHRVSLSMDKDQEIVDQFQ 120

Query: 121 DIQLRWRFVSEKKQKDDGYSKEKRHYELVFDKKFMDEVVDLYFPYILRRAKEIKEMENVA 180
           DI L+WRFV+EK ++D  + +EKRHYEL F KKFMD+VVD Y PYILRRAKEIK MENV+
Sbjct: 121 DIHLKWRFVAEKTKEDRQHIEEKRHYELSFHKKFMDKVVDFYLPYILRRAKEIKAMENVS 180

Query: 181 KLCSQNCSFNDDSGDDGCRGNWGSISLEHPATFDTLAMDPDLKKMILDDLDRFVKRKEFY 240
           KLCSQN S++DD GD+ CRGNWGSI LEHPATFDTLAMDPDLKKMI+DDLDRF+KRKEFY
Sbjct: 181 KLCSQNLSYSDDFGDERCRGNWGSIGLEHPATFDTLAMDPDLKKMIIDDLDRFLKRKEFY 240

Query: 241 RKVGKAWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLTDIDSNNELRRALLSTTNR 300
           RKVGKAWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLTDI SN+ELRR+LLST+NR
Sbjct: 241 RKVGKAWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLTDICSNSELRRSLLSTSNR 300

Query: 301 SILVIEDIDCSVNLQNREKGDEDNENLEPPRSRLTLSGMLNFMDGLWSSCGDERIIVLTT 360
           SILVIEDIDCSVNLQNR   D+++EN +  RS+LTLSGMLNFMDGLWSSCGDERIIVLTT
Sbjct: 301 SILVIEDIDCSVNLQNRANSDDESENNDDYRSKLTLSGMLNFMDGLWSSCGDERIIVLTT 360

Query: 361 NHKERLDPALLRPGRMDVHINLSYCTSKAFEVLATNYLGGGAIRHPLYEEIEGVIDYANV 420
           NHK+RLDPALLRPGRMDVHI LSYCTSKAFE L TNYLGGGAI HPLYEEI+ +I+YANV
Sbjct: 361 NHKDRLDPALLRPGRMDVHIYLSYCTSKAFEALTTNYLGGGAIHHPLYEEIKALIEYANV 420

Query: 421 TPAEVAEELMKGDDVNMVMEGLAKLVKVKIEEKSKVSDGNEAPEEEGEGIVEEGKEEKVK 480
           TPAEVAEELMK DD+++VMEGLAK VK+K EE+   + GN+APE+EG  +   G     +
Sbjct: 421 TPAEVAEELMKDDDIDIVMEGLAKFVKLKREEQ---NGGNKAPEKEGNEMEVVGG----R 480

Query: 481 RRKSGSGVRRYG 493
           RR+     + +G
Sbjct: 481 RRRKWESTKNHG 485

BLAST of Spg012426 vs. ExPASy TrEMBL
Match: A0A6J1KNM3 (AAA-ATPase At2g18193-like OS=Cucurbita maxima OX=3661 GN=LOC111497272 PE=3 SV=1)

HSP 1 Score: 724.2 bits (1868), Expect = 3.9e-205
Identity = 370/474 (78.06%), Postives = 423/474 (89.24%), Query Frame = 0

Query: 1   MFAFKEMPQSASSLFAAYASFATS-MMMIRSMTNDLLPPQLISFISSIFSYFFPPKSSPQ 60
           MF+FK+MP SASSLFAAYASFATS MMMIRS+TNDLLPP+L+SFISSIF+YFF PKSSP 
Sbjct: 1   MFSFKDMPPSASSLFAAYASFATSMMMMIRSITNDLLPPKLVSFISSIFNYFFTPKSSPH 60

Query: 61  TTLVIEKKTNYTKNQVYEAAEIYLRTKISPSMDRLKVSKTPRQQKVTLSMEKGQEIVDEF 120
           TTLVI+ KTNY KNQV++AAEIYLRTKISPSMDRL+ SKTPRQ +V+LSM+K QEIVD+F
Sbjct: 61  TTLVIKNKTNYAKNQVFDAAEIYLRTKISPSMDRLEASKTPRQHRVSLSMDKDQEIVDQF 120

Query: 121 QDIQLRWRFVSEKKQKDDGYSKEKRHYELVFDKKFMDEVVDLYFPYILRRAKEIKEMENV 180
           QDI L+WRFV+EKK++D  + +EK   +LVF KKF+D+VVD Y PYILRRAKEIK  ENV
Sbjct: 121 QDIHLKWRFVAEKKKEDRQHIEEKS--QLVFHKKFVDKVVDFYLPYILRRAKEIKATENV 180

Query: 181 AKLCSQNCSFNDDSGDDGCRGNWGSISLEHPATFDTLAMDPDLKKMILDDLDRFVKRKEF 240
           ++LCSQN  ++DDSG+D CRG+WGSI L HPATFDTLAMDPDLKKMI+D+LDRF+KRKEF
Sbjct: 181 SELCSQNLPYSDDSGEDRCRGHWGSIGLGHPATFDTLAMDPDLKKMIIDELDRFLKRKEF 240

Query: 241 YRKVGKAWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLTDIDSNNELRRALLSTTN 300
           YRKVGKAWKRGYLLYGPPGTGKSSL+AAMANYLKFDIYDLDLTDI SN++LRR+LLST+N
Sbjct: 241 YRKVGKAWKRGYLLYGPPGTGKSSLVAAMANYLKFDIYDLDLTDICSNSDLRRSLLSTSN 300

Query: 301 RSILVIEDIDCSVNLQNREKGDEDNENLEPPRSRLTLSGMLNFMDGLWSSCGDERIIVLT 360
           RSILVIEDIDCSVNLQNR   D+++EN +  RS+L LSGMLNFMDGLWSSCGDERIIVLT
Sbjct: 301 RSILVIEDIDCSVNLQNRANSDDESENNDDYRSKLMLSGMLNFMDGLWSSCGDERIIVLT 360

Query: 361 TNHKERLDPALLRPGRMDVHINLSYCTSKAFEVLATNYLGGGAIRHPLYEEIEGVIDYAN 420
           TNHK+RLD ALLRPGRMDVHINLSYCTSKAFE L TNYLGGGAI HPLYEEIE ++++AN
Sbjct: 361 TNHKDRLDAALLRPGRMDVHINLSYCTSKAFETLVTNYLGGGAIHHPLYEEIEALVEHAN 420

Query: 421 VTPAEVAEELMKGDDVNMVMEGLAKLVKVKIEEKSKVSDGNEAPEEEGEGIVEE 474
           VTPAEVAEELMK DD++++MEGLAK VK K EE+   +DGN A EEE   IVEE
Sbjct: 421 VTPAEVAEELMKDDDIDILMEGLAKFVKRKREEQ---NDGNMAAEEEVNEIVEE 469

BLAST of Spg012426 vs. ExPASy TrEMBL
Match: A0A1S3B1F9 (AAA-ATPase At2g18193-like OS=Cucumis melo OX=3656 GN=LOC103485084 PE=3 SV=1)

HSP 1 Score: 664.8 bits (1714), Expect = 2.8e-187
Identity = 337/478 (70.50%), Postives = 402/478 (84.10%), Query Frame = 0

Query: 1   MFAFKEMPQSASSLFAAYASFATSMMMIRSMTNDLLPPQLISFISSIFSYFFPPKSSPQT 60
           MF FK+MPQSASSLF AYASFAT+ MMIRSMT  LLPPQLIS ISS+  YFFPPKS+  T
Sbjct: 1   MFGFKDMPQSASSLFTAYASFATTAMMIRSMTTTLLPPQLISLISSVIFYFFPPKSTLIT 60

Query: 61  TLVIEKKTNYTKNQVYEAAEIYLRTKISPSMDRLKVSKTPRQQKVTLSMEKGQEIVDEFQ 120
           T+VI++K ++  NQ++EAA++YLRTKI+PSMDRLK SKTPRQ KV LSMEKGQ+IVD F+
Sbjct: 61  TIVIDQKCDFLNNQLFEAAQLYLRTKINPSMDRLKASKTPRQNKVALSMEKGQKIVDHFE 120

Query: 121 DIQLRWRFVSEKKQKDDGYSKEKRHYELVFDKKFMDEVVDLYFPYILRRAKEIKEMENVA 180
           DI+L+W FV+ +K+K + YS+EK HYELVF KKF+D VV+ YFPYIL+RAKEIK ++NVA
Sbjct: 121 DIRLQWGFVATQKEKRNEYSEEKCHYELVFPKKFLDRVVNFYFPYILQRAKEIKALDNVA 180

Query: 181 KLCSQNCSFNDDSGDDGCRGNWGSISLEHPATFDTLAMDPDLKKMILDDLDRFVKRKEFY 240
           KLCS +CS++D+S     +G WGS+ LEHPATFDTLA+DPDLKKMI+DDLDRFVKRKEFY
Sbjct: 181 KLCSSSCSYDDESLGGKRQGKWGSVRLEHPATFDTLALDPDLKKMIIDDLDRFVKRKEFY 240

Query: 241 RKVGKAWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLTDIDSNNELRRALLSTTNR 300
           RKVGKAWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDL+D+ SN  LR +LLSTTNR
Sbjct: 241 RKVGKAWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLSDVYSNQSLRSSLLSTTNR 300

Query: 301 SILVIEDIDCSVNLQNREKGDEDNENLEPPRSRLTLSGMLNFMDGLWSSCGDERIIVLTT 360
           SILVIEDIDCSV+LQNR    +  E  EPP+SRLTLSGMLNF+DGLWSSCGDERII+ TT
Sbjct: 301 SILVIEDIDCSVDLQNR----KFEEKFEPPKSRLTLSGMLNFIDGLWSSCGDERIIIFTT 360

Query: 361 NHKERLDPALLRPGRMDVHINLSYCTSKAFEVLATNYLGGGAIRHPLYEEIEGVIDYANV 420
           NHKE+LDPALLRPGRMDVHI+L YCTSK  +VLATNYLG     H LYEE++G+ID  N 
Sbjct: 361 NHKEKLDPALLRPGRMDVHIHLGYCTSKMLKVLATNYLGAEVTDHRLYEEVQGLIDCVNA 420

Query: 421 TPAEVAEELMKGDDVNMVMEGLAKLVKVKIEEKSKVSDGNEAPEEEGEGIVEEGKEEK 479
           TPAE+AEELMK DD+++V+EGLA  +K+K +E+       +A +E+ + I+EE K  +
Sbjct: 421 TPAEIAEELMKSDDIDVVIEGLANFLKLKSKER-------KAGDEKIDRILEENKASR 467

BLAST of Spg012426 vs. TAIR 10
Match: AT2G18193.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 497.7 bits (1280), Expect = 1.1e-140
Identity = 261/495 (52.73%), Postives = 357/495 (72.12%), Query Frame = 0

Query: 1   MFAFKEMPQSASSLFAAYASFATSMMMIRSMTNDLLPPQLISFISSIFSYFFPPKSSPQT 60
           MF   +   S SSLF+AYAS    +M+ RSM +D +P +L S+ SS+   FF PKS    
Sbjct: 1   MFPSSDFSFSPSSLFSAYASLTGFLMLFRSMLHDFVPEKLRSYFSSLLDRFFTPKSK-YL 60

Query: 61  TLVIEKKTNYTKNQVYEAAEIYLRTKISPSMDRLKVSKTPRQQKVTLSMEKGQEIVDEFQ 120
           T++I++     +NQV++AAE+YLR+KI P  +RL+V K P+Q+  T+S+E+G+EI+D F+
Sbjct: 61  TVIIDENFGLNRNQVFDAAEMYLRSKIGPETERLRVGKIPKQKHFTISIERGEEILDTFE 120

Query: 121 DIQLRWRFVSEKKQKDDGYSKEKRHYELVFDKKFMDEVVDLYFPYILRRAKEIKEMENVA 180
           + +++W +V  + +K D   K KR+YEL F+KK  D+V++ Y  +++  ++EIK    V 
Sbjct: 121 ESEVKWSYVQSENEKGD---KVKRYYELTFEKKLRDKVLNSYLTHVVAESEEIKRNLRVV 180

Query: 181 KLCSQNCSFNDDSGDDG-CRGNWGSISLEHPATFDTLAMDPDLKKMILDDLDRFVKRKEF 240
           KL S++   +DD  DDG   GNWG I+LEHP+TFDTLAMDP+ KK I+DDL+RF+KRKEF
Sbjct: 181 KLYSRDVYASDD--DDGMAGGNWGCINLEHPSTFDTLAMDPNAKKKIIDDLERFLKRKEF 240

Query: 241 YRKVGKAWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLTDIDSNNELRRALLSTTN 300
           Y++VGKAWKRGYLLYGPPGTGKSSLIAAMANYLKFD++DL+L+ I  N EL+R LLSTTN
Sbjct: 241 YKRVGKAWKRGYLLYGPPGTGKSSLIAAMANYLKFDVFDLELSSIYDNGELKRVLLSTTN 300

Query: 301 RSILVIEDIDCSVNLQNREKGDEDNENLEPPRSRLTLSGMLNFMDGLWSSCGDERIIVLT 360
           RSILVIEDIDC+  +++RE  ++++E +   + ++TLSG+LNF+DGLWSS GDERIIV T
Sbjct: 301 RSILVIEDIDCNAEVRDREAENQEDEQI---KGKVTLSGILNFIDGLWSSFGDERIIVFT 360

Query: 361 TNHKERLDPALLRPGRMDVHINLSYCTSKAFEVLATNYLGGGAIRHPLYEEIEGVIDYAN 420
           TNHKERLDPALLRPGRMDVHIN+SYCT   F  L +NYLG   + HPL EEIE ++D   
Sbjct: 361 TNHKERLDPALLRPGRMDVHINMSYCTGLGFRTLVSNYLGLDGLNHPLCEEIEALVDSTE 420

Query: 421 VTPAEVAEELMKGDDVNMVMEGLAKLVKVKIEEKSKVSDGNEAPEEEGEGIVEEGKEEKV 480
           VTPAE+AEELM+ DD ++V+ G+   V+ +  E+SK        +   +   + G    V
Sbjct: 421 VTPAELAEELMQDDDTDVVLRGVISFVEKRKVERSKTKKEVSICKATDDDEKQNGSLGCV 480

Query: 481 KRRKSGSGVRRYGRG 495
           K++K G   +  G+G
Sbjct: 481 KKKKKGGKQKGKGKG 486

BLAST of Spg012426 vs. TAIR 10
Match: AT2G18190.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 473.4 bits (1217), Expect = 2.3e-133
Identity = 256/483 (53.00%), Postives = 343/483 (71.01%), Query Frame = 0

Query: 10  SASSLFAAYASFATSMMMIRSMTNDLLPPQLISFISSIFSYFFPPKSSPQTTLVIEKKTN 69
           S SSLF AYAS    +M+ RS+ ND +P +L S+I+ + + FF PKS    T+VI++   
Sbjct: 11  SPSSLFTAYASLTGFLMLFRSLFNDEVPERLRSYITDLLNRFFTPKSK-NLTMVIDEIIG 70

Query: 70  YTKNQVYEAAEIYLRTKISPSMDRLKVSKTPRQQKVTLSMEKGQEIVDEFQDIQLRWRFV 129
           + +NQV++AAE+YLR KI P   RL+V K P+Q+  T+ +EKG+EI+D F++ +LRW +V
Sbjct: 71  FKRNQVFDAAEVYLRNKIGPETARLRVGKLPKQKHFTIYIEKGEEILDTFENSELRWTYV 130

Query: 130 SEKKQKDDGYSKEKRHYELVFDKKFMDEVVDLYFPYILRRAKEIKEMENVAKLCSQNCSF 189
              + +++   KEKR+YEL F+KK  D+V++ Y  +++  ++E K      KL S++   
Sbjct: 131 ---ESENEASQKEKRYYELTFEKKLRDKVMNSYLSHVVAESEETKRDLRAVKLYSRDVRA 190

Query: 190 NDDSGDDGCRG-NWGSISLEHPATFDTLAMDPDLKKMILDDLDRFVKRKEFYRKVGKAWK 249
           + D  DDG  G  WG I+LEHP+TF+TLAMDP  KK I+DD++RF+KR+EFY++VGKAWK
Sbjct: 191 SKD--DDGMAGAGWGCINLEHPSTFETLAMDPGAKKKIIDDMERFLKRREFYKRVGKAWK 250

Query: 250 RGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLTDIDSNNELRRALLSTTNRSILVIEDI 309
           RGYLLYGPPGTGKSSLIAAMANYLKFD++DL+L+ I  N +L+  LLSTTNRSILVIEDI
Sbjct: 251 RGYLLYGPPGTGKSSLIAAMANYLKFDVFDLELSSIYENAQLKSILLSTTNRSILVIEDI 310

Query: 310 DCSVNLQNREKGDEDNENLEPPRSRLTLSGMLNFMDGLWSSCGDERIIVLTTNHKERLDP 369
           DCS       + DE  E  E    R+TLSG+LNF+DGLWSS GDERIIV TTNHKERLDP
Sbjct: 311 DCSSAEVVDREADEYQEYEEGYYGRVTLSGLLNFVDGLWSSFGDERIIVFTTNHKERLDP 370

Query: 370 ALLRPGRMDVHINLSYCTSKAFEVLATNYLGGGAIRHPLYEEIEGVIDYANVTPAEVAEE 429
           ALLRPGRMD+HIN+SYCT   F  L +NYLG G + HPL EEIE +ID   VTPAE+AEE
Sbjct: 371 ALLRPGRMDMHINMSYCTGLGFRTLVSNYLGLGGLNHPLCEEIEALIDSTEVTPAELAEE 430

Query: 430 LMKGDDVNMVMEGLAKLV---KVKIEEKSKVSDGNEAPEEEGE---GIVEEGKEEKVKRR 486
           LM+ DD ++V+ G+   V   KV+I  K+K  +G+   + +G+    +      +K K++
Sbjct: 431 LMQEDDTDVVLRGVVSFVENRKVEI-SKTKELEGSTCRKLDGDDKHNVSSTNDLKKTKKK 486

BLAST of Spg012426 vs. TAIR 10
Match: AT5G17760.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 429.5 bits (1103), Expect = 3.8e-120
Identity = 241/496 (48.59%), Postives = 328/496 (66.13%), Query Frame = 0

Query: 1   MFAFKEMPQSASSLFAAYASFATSMMMIRSMTNDLLPPQLISFISSIFSYFFPPKSSPQT 60
           MF  K++P S +S+F AYAS A  MMMIRSM ++L+P  L  FI       F   SS   
Sbjct: 1   MFFSKDLP-SPTSVFTAYASMAGYMMMIRSMAHELIPAPLQDFIYRTLRSLFFRSSSSTL 60

Query: 61  TLVIEKKTNYTKNQVYEAAEIYLRTKISPSMDRLKVSKTPRQQKVTLSMEKGQEIVDEFQ 120
           TL I+       N++Y AA+ YL TKISP   RL++SK  + + V L +  G+ + D ++
Sbjct: 61  TLTIDDDNMGMNNEIYRAAQTYLSTKISPDAVRLRISKGHKDKHVNLYLSDGEIVNDVYE 120

Query: 121 DIQLRWRFVSEKKQK---------------------DDGYSKEKRHYELVFDKKFMDEVV 180
           D+QL WRFV++   K                     DDG S+   ++EL FDKK  D ++
Sbjct: 121 DVQLVWRFVTDGGDKKGGGGGVGGRGGGGGRRGGMDDDGKSE---YFELSFDKKHKDLIL 180

Query: 181 DLYFPYILRRAKEIKEMENVAKLCSQNCSFNDDSGDDGCRGNWGSISLEHPATFDTLAMD 240
           + Y PYI  +AKEI++   +  L S N               W S+ LEHP+TF+T+AM+
Sbjct: 181 NSYVPYIESKAKEIRDERRILMLHSLNSL------------RWESVILEHPSTFETMAME 240

Query: 241 PDLKKMILDDLDRFVKRKEFYRKVGKAWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDL 300
            DLK+ +++DLDRF++RKEFY++VGKAWKRGYLLYGPPGTGKSSL+AAMANYLKFD+YDL
Sbjct: 241 DDLKRDVIEDLDRFIRRKEFYKRVGKAWKRGYLLYGPPGTGKSSLVAAMANYLKFDVYDL 300

Query: 301 DLTDIDSNNELRRALLSTTNRSILVIEDIDCSVNLQNREKGDEDNENLEPPRSRLTLSGM 360
            L  +  +++LRR LL+T NRSILVIEDIDC+V+L NR +   + +N    +  LTLSG+
Sbjct: 301 QLASVMRDSDLRRLLLATRNRSILVIEDIDCAVDLPNRIEQPVEGKNRGESQGPLTLSGL 360

Query: 361 LNFMDGLWSSCGDERIIVLTTNHKERLDPALLRPGRMDVHINLSYCTSKAFEVLATNYLG 420
           LNF+DGLWSSCGDERII+ TTNHK+RLDPALLRPGRMD+HI + +C+ + F+ LA+NYLG
Sbjct: 361 LNFIDGLWSSCGDERIIIFTTNHKDRLDPALLRPGRMDMHIYMGHCSFQGFKTLASNYLG 420

Query: 421 --GGAIRHPLYEEIEGVIDYANVTPAEVAEELMKGDDVNMVMEGLAKLVKVKIEEKSKVS 474
               A+ H L+ EIE +ID   +TPA+VAEELMK +D ++ +EGL  +++ K+  KSK S
Sbjct: 421 LSDAAMPHRLFPEIERLIDGEVMTPAQVAEELMKSEDADVALEGLVNVLE-KMRLKSKES 479

BLAST of Spg012426 vs. TAIR 10
Match: AT3G50940.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 414.5 bits (1064), Expect = 1.3e-115
Identity = 226/449 (50.33%), Postives = 305/449 (67.93%), Query Frame = 0

Query: 10  SASSLFAAYASFATSMMMIRSMTNDLLPPQLISFISSIFSYFFPPKSSPQTTLVIEKKTN 69
           +A +   A AS A + ++ RS+  D +P ++  +IS  F  FF    S Q T VIE+   
Sbjct: 11  TAKTALTAVASVAAAAILARSVVQDYMPNEVHEYISHGFRRFF-SYFSYQMTAVIEEFGG 70

Query: 70  YTKNQVYEAAEIYLRTKISPSMDRLKVSKTPRQQKVTLSMEKGQEIVDEFQDIQLRWRFV 129
           +  NQV+EAAE YL TKIS S  R+KV+K  +Q   ++++E+ +E+VD F  ++L W  V
Sbjct: 71  FEHNQVFEAAEAYLSTKISNSTRRIKVNKLEKQSNYSVTVERDEEVVDIFDGVKLSWILV 130

Query: 130 SEKKQKDD---------GYSKEKRHYELVFDKKFMDEVVDLYFPYILRRAKEIKEMENVA 189
                K D             E R YEL F KKF + V++ Y P+++ +A  IK+     
Sbjct: 131 CRHVDKKDFRNPRDLNSTLKSEVRSYELSFRKKFKNMVLESYLPFVVEQAASIKQKFKTL 190

Query: 190 KLCSQNCSFNDDSGDDGCRGNWGSISLEHPATFDTLAMDPDLKKMILDDLDRFVKRKEFY 249
           K+ +           D     W S++L+HP+TF TLA+DP++KK +++DLDRFV+RK FY
Sbjct: 191 KIFTV----------DSYSVEWTSVTLDHPSTFRTLALDPEVKKNLVEDLDRFVQRKGFY 250

Query: 250 RKVGKAWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLTDIDSNNELRRALLSTTNR 309
            +VGKAWKRGYLLYGPPGTGKSSLIAA+AN+L FDIYDLDLT +++N ELRR L+ST NR
Sbjct: 251 GRVGKAWKRGYLLYGPPGTGKSSLIAAIANHLNFDIYDLDLTSLNNNAELRRLLMSTANR 310

Query: 310 SILVIEDIDCSVNLQNREKGDEDNENLEPPRSRLTLSGMLNFMDGLWSSCGDERIIVLTT 369
           SILV+EDIDCS+ L++R     D EN +P    +TLSG+LNF+DGLWSSCG+ERIIV TT
Sbjct: 311 SILVVEDIDCSIELKDR---STDQENNDPLHKTVTLSGLLNFVDGLWSSCGNERIIVFTT 370

Query: 370 NHKERLDPALLRPGRMDVHINLSYCTSKAFEVLATNYLGGGAIRHPLYEEIEGVIDYANV 429
           N++E+LDPALLRPGRMD+HI++SYCT  AF+VLA+NYL      H L+E+IE  I    V
Sbjct: 371 NYREKLDPALLRPGRMDMHIHMSYCTPAAFKVLASNYL--EIQDHILFEQIEEFIREIEV 430

Query: 430 TPAEVAEELMKGDDVNMVMEGLAKLVKVK 450
           TPAEVAE+LM+ D V+ V++GL + +K K
Sbjct: 431 TPAEVAEQLMRSDSVDKVLQGLVEFLKAK 443

BLAST of Spg012426 vs. TAIR 10
Match: AT3G50930.1 (cytochrome BC1 synthesis )

HSP 1 Score: 412.9 bits (1060), Expect = 3.7e-115
Identity = 230/496 (46.37%), Postives = 332/496 (66.94%), Query Frame = 0

Query: 10  SASSLFAAYASFATSMMMIRSMTNDLLPPQLISFIS----SIFSYFFPPKSSPQTTLVIE 69
           +A ++    AS A + M+ RS+  D LP ++  +IS    SIF YF     S Q T++IE
Sbjct: 57  TAKTVLTTAASVAATAMLARSLVQDYLPDEVHHYISYGFRSIFGYF-----SSQMTIIIE 116

Query: 70  KKTNYTKNQVYEAAEIYLRTKISPSMDRLKVSKTPRQQKVTLSMEKGQEIVDEFQDIQLR 129
           +   +  N+V+EAAE YL TKISPS  R+KVSK  ++    +++E+ +E+VD +  ++ +
Sbjct: 117 EFEGFAHNEVFEAAEAYLATKISPSNKRIKVSKHEKENNYNVTVERDEEVVDTYNGVKFQ 176

Query: 130 W----RFVSEK-----KQKDDGYSKEKRHYELVFDKKFMDEVVDLYFPYILRRAKEIKEM 189
           W    R V  K     +  +     E R +EL F KKF D  ++ Y P++++RA  +K+ 
Sbjct: 177 WILHCRHVESKHFHNPRDLNSTLRSEVRSFELNFHKKFKDVALESYLPFMVKRATLMKQE 236

Query: 190 ENVAKLCSQNCSFNDDSGDDGCRGNWGSISLEHPATFDTLAMDPDLKKMILDDLDRFVKR 249
           +   K+ + +      +  D     W S++L+HP+TF TLAMD D+K  +++DLD+FVKR
Sbjct: 237 KKTLKIFTLSPENMYGNYSDA----WTSVTLDHPSTFKTLAMDSDVKTSVMEDLDKFVKR 296

Query: 250 KEFYRKVGKAWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLTDIDSNNELRRALLS 309
           ++FY++VGKAWKRGYLLYGPPGTGKSSLIAAMAN+L FDIYDL+LT +++N+ELRR L++
Sbjct: 297 RDFYKRVGKAWKRGYLLYGPPGTGKSSLIAAMANHLNFDIYDLELTAVNNNSELRRLLIA 356

Query: 310 TTNRSILVIEDIDCSVNLQNR--EKGDEDNENLEPPR-SRLTLSGMLNFMDGLWSSCGDE 369
           T NRSIL++EDIDCS+ L++R  ++   +++++E PR  ++TLSG+LNF+DGLWSSCGDE
Sbjct: 357 TANRSILIVEDIDCSLELKDRTSDEPPRESDDIEDPRYKKVTLSGLLNFIDGLWSSCGDE 416

Query: 370 RIIVLTTNHKERLDPALLRPGRMDVHINLSYCTSKAFEVLATNYLGGGAIRHPLYEEIEG 429
           RII+ TTN+KE+LD ALLRPGRMD+HI++SYCT   F+ LA NYL      H L+ +IE 
Sbjct: 417 RIIIFTTNYKEKLDAALLRPGRMDMHIHMSYCTPSTFKALALNYL--EIKEHRLFSKIEE 476

Query: 430 VIDYANVTPAEVAEELMKGDDVNMVMEGLAKLVKVKIEEKSKVSDGNEAPEEEGEGIVEE 489
            I+   VTPAEVAE+LM+ D V+ V+EGL + +KVK  E  +     E  E E +   +E
Sbjct: 477 GIEATEVTPAEVAEQLMRNDSVDKVLEGLIEFLKVKKIENEQDKAKTEKQELENKKKTKE 536

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022132940.12.1e-22982.39AAA-ATPase At2g18193-like [Momordica charantia][more]
XP_023517746.19.5e-22283.30AAA-ATPase At2g18193-like [Cucurbita pepo subsp. pepo][more]
XP_022962680.11.2e-22183.51AAA-ATPase At2g18193-like [Cucurbita moschata][more]
KAG6594808.11.4e-21782.42Phosphatidylinositol/phosphatidylcholine transfer protein SFH12, partial [Cucurb... [more]
XP_022962730.14.0e-21277.85AAA-ATPase At2g18193-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q8GW961.6e-13952.73AAA-ATPase At2g18193 OS=Arabidopsis thaliana OX=3702 GN=At2g18193 PE=2 SV=1[more]
F4IQG23.2e-13253.00AAA-ATPase At2g18190 OS=Arabidopsis thaliana OX=3702 GN=At2g18190 PE=2 SV=1[more]
Q9FN755.4e-11948.59AAA-ATPase At5g17760 OS=Arabidopsis thaliana OX=3702 GN=At5g17760 PE=3 SV=1[more]
Q147F91.8e-11450.33AAA-ATPase At3g50940 OS=Arabidopsis thaliana OX=3702 GN=At3g50940 PE=2 SV=1[more]
Q8VZG25.2e-11446.37Protein HYPER-SENSITIVITY-RELATED 4 OS=Arabidopsis thaliana OX=3702 GN=HSR4 PE=2... [more]
Match NameE-valueIdentityDescription
A0A6J1BTY01.0e-22982.39AAA-ATPase At2g18193-like OS=Momordica charantia OX=3673 GN=LOC111005660 PE=3 SV... [more]
A0A6J1HFS76.0e-22283.51AAA-ATPase At2g18193-like OS=Cucurbita moschata OX=3662 GN=LOC111463099 PE=3 SV=... [more]
A0A6J1HFX61.9e-21277.85AAA-ATPase At2g18193-like OS=Cucurbita moschata OX=3662 GN=LOC111463134 PE=3 SV=... [more]
A0A6J1KNM33.9e-20578.06AAA-ATPase At2g18193-like OS=Cucurbita maxima OX=3661 GN=LOC111497272 PE=3 SV=1[more]
A0A1S3B1F92.8e-18770.50AAA-ATPase At2g18193-like OS=Cucumis melo OX=3656 GN=LOC103485084 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT2G18193.11.1e-14052.73P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
AT2G18190.12.3e-13353.00P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
AT5G17760.13.8e-12048.59P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
AT3G50940.11.3e-11550.33P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
AT3G50930.13.7e-11546.37cytochrome BC1 synthesis [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003593AAA+ ATPase domainSMARTSM00382AAA_5coord: 247..386
e-value: 2.1E-7
score: 40.6
IPR025753AAA-type ATPase, N-terminal domainPFAMPF14363AAA_assoccoord: 36..129
e-value: 1.8E-22
score: 79.5
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 204..380
e-value: 3.8E-44
score: 152.6
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 169..430
IPR003959ATPase, AAA-type, corePFAMPF00004AAAcoord: 252..383
e-value: 3.5E-18
score: 66.3
NoneNo IPR availableGENE3D6.10.280.40coord: 381..456
e-value: 1.0E-15
score: 59.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 453..511
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 484..500
NoneNo IPR availablePANTHERPTHR23070:SF160SUBFAMILY NOT NAMEDcoord: 4..469
NoneNo IPR availablePANTHERPTHR23070BCS1 AAA-TYPE ATPASEcoord: 4..469
NoneNo IPR availableCDDcd00009AAAcoord: 248..384
e-value: 1.16981E-12
score: 63.7043
IPR003960ATPase, AAA-type, conserved sitePROSITEPS00674AAAcoord: 354..372

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg012426.1Spg012426.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016020 membrane
molecular_function GO:0005524 ATP binding
molecular_function GO:0016887 ATP hydrolysis activity