Spg012223 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg012223
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial
Location	scaffold1: 7913019 .. 7916374 (-)
RNA-Seq Expression	Spg012223
Synteny	Spg012223

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AATATAAAAGTTTTCGTATTAGTTTAAAGGCGCACCTGTAATTTCTACAAAGTTTTAGAGAAAACAACAGCGTAAATTTCTTCTCCATCTTTCGAAGCATAATCGAGTAGGAACTGTGTTTTTAACGGGGGACGAGCCTGACGGAAAACTAGTTTTCGCGTCGAATTTCGTTTATCGACCGATGGCTAACTCTCTGCAGCGAATCTGGAACCACAGATATGCTCTCCGTGGTACTGTGGCTCCTCTTTGCAGGTCATTTTCCGCCGACTCCTTGGTTGAAGTCAAGCCCGGCGAGATCGGTATGGTCTCTGGAATTCCCGACGAACACCTTACCAGGAAGGTATTGCTCTGGCATTCATTTGCTTTACTGTTTATCAACGAGTTTCTGATTGCTCACTTTTAGTTTAATTTCGAGGGACCGGGTTATTAGATGGTATTGATGATTTTTGGGGATTAGGTATTTCTATACCCGTCTGGTTGGGTATTCTTGCTTGTTTTTACCTGGGCCTTTGGAATGTTCTGCAGTGGCTAAATCGTTTAGGTATTTCTTTGTTGCAACTATGTTGAAATGTAGTCGATTCTACAAGGGAGATGGAAATTTCCGGCTGATTTATTACTATGACACTATCATTTCTATTATCTTGATGACCAAAAATTCACTTAAATACGGTAAAAATGGTAGAGGAACCTGACGTTCAATCACTAAGAAAAACTGCACCTTTTCTTCTTCTTTTTTTATGTTGAATTATCAATTTTTAAGTTATGGAAGGTAAATGTTGGGATTTTTTTTAAAAGAATAAATATTTTGGAACTTTGCATGTATTGAGGTTTAATCTTGTACTTCTTATATGCGAACCACTATTATTGGATTTCCCTGTAATATTAATTATTTTTTCTTTCTATACTACCATCTCGATTCATTTGTCTTCCAATTATGAAATTATGTCAGGTTGTAATTTACTCACCTGCTAGAACTGCATCTCAGCAAGGATCAGGGAAGGTTGGCAAGTGGAAAATCAATTTTATGTCAACACAAAAGTATGAATCGTTCTTTTCTTTCTTTGTTCTCTGAGTTTTTTTTTTTTTTTCTAAAAAATCTAAAATTGCTCCATAAGTTAACAGAATTTTTAACTTAGTTCTAGCAGATTCCTAGGTGCTAGGGTGAAATATTGTGCACAATATGTTATACTGGGCAAACTTCAAGTGCACAAAAGTGAATGACTTGTTCTCTTACTCTCAACAATTAGAGTTTTTTCCCTTATTATCTTTTTCTCTTTTTGGACTTTTGTTTCTCTGATCAGGCATCTTATGTGGTGGATTGATTTCTTTCTTTATTGCCCGCGTGTAATATTTCATTTATTTTCATAAAAGTTTGATTTCTCATTTAAAACAGAAAAACATATAAACAGAAGGGTAAAGAGTTTTTTAATGCTTATCGACATGTTTGTAGGTGGGAAAATCCATTGATGGGGTGGACTTCTACTGGGGACCCCTATGCCAATGTAGGGGATTCTGCACTGTCTTTTGATAGTGAAGAAGCTGCAAAGGCATTTGCTGAGAAACATGGATGGGAATATGTGGTAATGCTTCGTTCAACCATTTAGAAAAAATTTTCTTCTTGTAACTCCCTTTTTGTAGTTTTAGTTGTCATATCTTAGGTTAGTTATGAACTCTGTTCGTTTTTTTTCCACTGGCAGTTGCCTTCAAATAAGTAATAATTATAGTTGGTGGTGTTGATTAATTATGCGAATGTTCTAATATATTCTTTACAATAGATCTTTGCTTTTCCACAAGGTTAAAACAATGCACTCTAATTCTAAATAAATAATAAATGCTAAAATGAAATTGCATTTGGATATGGTTGTTCGATATGTTTATTTAGAATAACAAATATGATTCTTGATTTTTGCATTTGGGCTATAGTATGACATTTGACTTTGAATGTAAGATCTTATCTTTGTAAATAGTAGAAATGAATTTAGTTATTTTATATCCTAGTATTAGTTTGAACTAAACTTCAGGAATTTCAAGAAGAGGAATTTGAGAGGGAGAAATGGCAGTCTTTCTTTTCAAATTTGGTCCATTAGATGCAGAAAGTTCATGTTGCTTATTTAGCATTCTGATGGCATTGCAGTACATTTTTAATTGTTTCTTTAGTGTTCAGATTGAGGAATCACTTTGCTGGCCTGCATATAATTTTCAGATCACATGGGGTTAATTTCTAATTCATCTGTTGATCATGTTTCTTGATTCTTTTTTTCTTTTTCTCCTTTTTGTAGGTTAAGAAGCGCCACACACCACTTTTGAAGGTTTGAGATTCTTTTCCATTATTTTAATTTCATTGGGCTTGTATTTTGTCTATAGTTCTGTGAATGTCTTTTTTGGGAGAAGAAGGGGAAGGGGGAAGGGGTGGTTTGCCCGCCCTCCATAGTGTTCATGTTGAAGCTAAGAGTGATTCATCCATTGAGCTCTCTGTCATATCTACAAGCTTCGACTATTTTCAAGGAATGATCCCTTGTGTTAAATTTTATTCCTTTGTTCAATGCAACTTCTTTCTTCATCTTTTAGAGTTTTTCTAGCACTCAATTTATTGTTTCCTCCCATTTTTGTGGCCTTCAAGACATTTTGTATGAGAATTCTCAATCCTTTTGCAATCTAGAGTCTTCTTGTGGCCAATATAAGGGGGTCAGTTTCAGTGTTCTTTTGGTACCAATTATTGTTTAGTAGTATATCCTAATTCTTTCCCAGATGGCATTATATAAGTTTGGGTTGTTACTATTGGAATAGTTTAGATATCCTAACAACCAAGAGTTCCTTTTAGATAGATGAGGAAGGAGGAGGATGAAGAAATGACAAATTGGTTTTAAAGCCAATCATGTTAACATCCAATTGACTAAAAGGAATTTTTTATGCTGCTTATTTTCTTTATATCATCTAAGGACATATTCAGTTAATCTTAGCTGGTTCACTGTGAGCACCAAAATAAAGAAACAAAATATATATATAACTCCTTGTTTTGTTTTGGCTGCAGGTGAAGGCATATGCTGACAATTTCAAATGGAAGGGCCCCCCTGAAGCCAAGGAGGCTTAAACCTTGTTCAACACTCCTTTTCTCATTCTAATTCAAGGCGGAACAGACTGGAGGGTTTGTGTAGTCCATTTCGTTGCTTATTTTTGAGGAAGTTGGTTATAATAAGTGGGAACCATGGTGTATACTCACTTGACCCTGTAACTTTTGTTACCTCTATATGTTGCCGTTACAGAACATCAGATGAAGCTCGTATACATTTGCAACCCCCATTTCGAGTAACAAATGAGCATAGTAATTGATAAGTTGATTGTTTTGTGCAATGGAG

mRNA sequence

ATGGCTAACTCTCTGCAGCGAATCTGGAACCACAGATATGCTCTCCGTGGTACTGTGGCTCCTCTTTGCAGGTCATTTTCCGCCGACTCCTTGGTTGAAGTCAAGCCCGGCGAGATCGGTATGGTCTCTGGAATTCCCGACGAACACCTTACCAGGAAGGTTGTAATTTACTCACCTGCTAGAACTGCATCTCAGCAAGGATCAGGGAAGGTTGGCAAGTGGGAAAATCCATTGATGGGGTGGACTTCTACTGGGGACCCCTATGCCAATGTAGGGGATTCTGCACTGTCTTTTGATAGTGAAGAAGCTGCAAAGGCATTTGCTGAGAAACATGGATGGGAATATGTGGTTAAGAAGCGCCACACACCACTTTTGAAGGTGAAGGCATATGCTGACAATTTCAAATGGAAGGGCCCCCCTGAAGCCAAGGAGGCTTAA

Coding sequence (CDS)

ATGGCTAACTCTCTGCAGCGAATCTGGAACCACAGATATGCTCTCCGTGGTACTGTGGCTCCTCTTTGCAGGTCATTTTCCGCCGACTCCTTGGTTGAAGTCAAGCCCGGCGAGATCGGTATGGTCTCTGGAATTCCCGACGAACACCTTACCAGGAAGGTTGTAATTTACTCACCTGCTAGAACTGCATCTCAGCAAGGATCAGGGAAGGTTGGCAAGTGGGAAAATCCATTGATGGGGTGGACTTCTACTGGGGACCCCTATGCCAATGTAGGGGATTCTGCACTGTCTTTTGATAGTGAAGAAGCTGCAAAGGCATTTGCTGAGAAACATGGATGGGAATATGTGGTTAAGAAGCGCCACACACCACTTTTGAAGGTGAAGGCATATGCTGACAATTTCAAATGGAAGGGCCCCCCTGAAGCCAAGGAGGCTTAA

Protein sequence

MANSLQRIWNHRYALRGTVAPLCRSFSADSLVEVKPGEIGMVSGIPDEHLTRKVVIYSPARTASQQGSGKVGKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEKHGWEYVVKKRHTPLLKVKAYADNFKWKGPPEAKEA

Homology

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BLAST of Spg012223 vs. NCBI nr
Match: XP_022132610.1 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial [Momordica charantia])

HSP 1 Score: 283.9 bits (725), Expect = 7.9e-73
Identity = 138/155 (89.03%), Postives = 143/155 (92.26%), Query Frame = 0


Query: 1   MANSLQRIWNHRYALRGTVAPLCRSFSADSLVEVKPGEIGMVSGIPDEHLTRKVVIYSPA 60
           MANSLQRIWNHR  LRGT+APLCRSFSADSLV+VKPGEIGMVSGIP+EHLTR+VVIYSPA
Sbjct: 1   MANSLQRIWNHRCGLRGTLAPLCRSFSADSLVQVKPGEIGMVSGIPEEHLTRRVVIYSPA 60

Query: 61  RTASQQGSGKVG----------KWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEK 120
           RTASQQGSGKVG          KWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEK
Sbjct: 61  RTASQQGSGKVGKWKINFMSTQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEK 120

Query: 121 HGWEYVVKKRHTPLLKVKAYADNFKWKGPPEAKEA 146
           HGW+YVVKKRHTPLLKVKAYADNFKWKGPPEAKEA
Sbjct: 121 HGWDYVVKKRHTPLLKVKAYADNFKWKGPPEAKEA 155

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BLAST of Spg012223 vs. NCBI nr
Match: XP_038881462.1 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial [Benincasa hispida])

HSP 1 Score: 279.6 bits (714), Expect = 1.5e-71
Identity = 136/155 (87.74%), Postives = 142/155 (91.61%), Query Frame = 0


Query: 1   MANSLQRIWNHRYALRGTVAPLCRSFSADSLVEVKPGEIGMVSGIPDEHLTRKVVIYSPA 60
           MANSLQRIW+H  ALRGTVAPLCRSFS DSLV+VKPGEIGMVSGIP+EHLTR+V+IYSPA
Sbjct: 1   MANSLQRIWSHGCALRGTVAPLCRSFSTDSLVQVKPGEIGMVSGIPEEHLTRRVIIYSPA 60

Query: 61  RTASQQGSGKVG----------KWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEK 120
           RTASQQGSGKVG          KWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEK
Sbjct: 61  RTASQQGSGKVGKWKINFMSTQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEK 120

Query: 121 HGWEYVVKKRHTPLLKVKAYADNFKWKGPPEAKEA 146
           HGWEYVV+KRHTPLLKVKAYADNFKWKGPPEAKEA
Sbjct: 121 HGWEYVVQKRHTPLLKVKAYADNFKWKGPPEAKEA 155

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BLAST of Spg012223 vs. NCBI nr
Match: XP_022962977.1 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial-like [Cucurbita moschata] >XP_022962978.1 NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial-like [Cucurbita moschata] >XP_023003205.1 NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial-like [Cucurbita maxima] >XP_023517797.1 NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial-like [Cucurbita pepo subsp. pepo] >KAG6594972.1 NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 275.0 bits (702), Expect = 3.7e-70
Identity = 136/155 (87.74%), Postives = 139/155 (89.68%), Query Frame = 0


Query: 1   MANSLQRIWNHRYALRGTVAPLCRSFSADSLVEVKPGEIGMVSGIPDEHLTRKVVIYSPA 60
           MANSLQRIWN   ALRGT+APLCRSFSADSLVEVKPGEIGMVSGIP+EHLTR+VVIYSPA
Sbjct: 1   MANSLQRIWNRGCALRGTMAPLCRSFSADSLVEVKPGEIGMVSGIPEEHLTRRVVIYSPA 60

Query: 61  RTASQQGSGKVG----------KWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEK 120
           RTASQQGSGKVG          KWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEK
Sbjct: 61  RTASQQGSGKVGKWKINFMSTQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEK 120

Query: 121 HGWEYVVKKRHTPLLKVKAYADNFKWKGPPEAKEA 146
           HGWEYVVKKRHTPLLK KAYADNFKWKG PE KEA
Sbjct: 121 HGWEYVVKKRHTPLLKAKAYADNFKWKGHPEPKEA 155

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BLAST of Spg012223 vs. NCBI nr
Match: XP_022977437.1 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial-like [Cucurbita maxima])

HSP 1 Score: 273.5 bits (698), Expect = 1.1e-69
Identity = 134/155 (86.45%), Postives = 141/155 (90.97%), Query Frame = 0


Query: 1   MANSLQRIWNHRYALRGTVAPLCRSFSADSLVEVKPGEIGMVSGIPDEHLTRKVVIYSPA 60
           MANSLQRIW++  ALRGTVAPLCRSFS+DSLV++KPGEIGMVSGIPDEHLTR+VVIYSPA
Sbjct: 1   MANSLQRIWSNSRALRGTVAPLCRSFSSDSLVQLKPGEIGMVSGIPDEHLTRRVVIYSPA 60

Query: 61  RTASQQGSGKVG----------KWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEK 120
           RTASQQGSGKVG          KWENPLMGWTSTGDPYANVGDSALSFDSEEAAK FA+K
Sbjct: 61  RTASQQGSGKVGKWKINFMSTQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKEFADK 120

Query: 121 HGWEYVVKKRHTPLLKVKAYADNFKWKGPPEAKEA 146
           HGWEYVVKKRHTPLLKVKAYADNFKWKG PEAKEA
Sbjct: 121 HGWEYVVKKRHTPLLKVKAYADNFKWKGHPEAKEA 155

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BLAST of Spg012223 vs. NCBI nr
Match: XP_022950018.1 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial-like [Cucurbita moschata] >KAG6604122.1 NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 272.7 bits (696), Expect = 1.8e-69
Identity = 133/155 (85.81%), Postives = 141/155 (90.97%), Query Frame = 0


Query: 1   MANSLQRIWNHRYALRGTVAPLCRSFSADSLVEVKPGEIGMVSGIPDEHLTRKVVIYSPA 60
           MANSLQRIW++  A+RGTVAPLCRSFS+DSLV++KPGEIGMVSGIPDEHLTR+VVIYSPA
Sbjct: 1   MANSLQRIWSNSRAIRGTVAPLCRSFSSDSLVQLKPGEIGMVSGIPDEHLTRRVVIYSPA 60

Query: 61  RTASQQGSGKVG----------KWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEK 120
           RTASQQGSGKVG          KWENPLMGWTSTGDPYANVGDSALSFDSEEAAK FA+K
Sbjct: 61  RTASQQGSGKVGKWKINFMSTQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKEFADK 120

Query: 121 HGWEYVVKKRHTPLLKVKAYADNFKWKGPPEAKEA 146
           HGWEYVVKKRHTPLLKVKAYADNFKWKG PEAKEA
Sbjct: 121 HGWEYVVKKRHTPLLKVKAYADNFKWKGHPEAKEA 155

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BLAST of Spg012223 vs. ExPASy Swiss-Prot
Match: Q9FJW4 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=FRO1 PE=1 SV=1)

HSP 1 Score: 194.5 bits (493), Expect = 8.3e-49
Identity = 94/139 (67.63%), Postives = 113/139 (81.29%), Query Frame = 0


Query: 15  LRGTVAPLCRSFSADSLVE--VKPGEIGMVSGIPDEHLTRKVVIYSPARTASQQGSGKVG 74
           +  T+  + R F+ D++VE   K GEIG VSGIP+EHL+RKV+IYSPARTA+Q GSGK+G
Sbjct: 13  IAATLRRVARPFATDAVVESDYKRGEIGKVSGIPEEHLSRKVIIYSPARTATQSGSGKLG 72

Query: 75  ----------KWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEKHGWEYVVKKRHT 134
                     KWENPLMGWTSTGDPYANVGDSAL+FDSEEAAK+FAE+HGW+Y VKK +T
Sbjct: 73  KWKINFVSTLKWENPLMGWTSTGDPYANVGDSALAFDSEEAAKSFAERHGWDYKVKKPNT 132

Query: 135 PLLKVKAYADNFKWKGPPE 142
           PLLKVK+Y+DNFKWKG P+
Sbjct: 133 PLLKVKSYSDNFKWKGNPQ 151

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BLAST of Spg012223 vs. ExPASy Swiss-Prot
Match: Q5XIF3 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Rattus norvegicus OX=10116 GN=Ndufs4 PE=1 SV=1)

HSP 1 Score: 96.3 bits (238), Expect = 3.1e-19
Identity = 53/121 (43.80%), Postives = 69/121 (57.02%), Query Frame = 0


Query: 27  SADSLVEVKPGEIGMVSGIPDEHL-TRKVVIYSPARTASQQGSGKVGK----------WE 86
           + D  ++V P     ++G+P+EH+ TRKV I+ PAR   Q G     K          WE
Sbjct: 54  TVDEKLDVTP-----LTGVPEEHIKTRKVRIFVPARNNMQSGVNNTKKWKMEFDTRERWE 113

Query: 87  NPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEKHGWEYVVKKRHTPLLKVKAYADNFK 137
           NPLMGW ST DP +N+    L+F ++E A AFAEKHGW Y V+ R  P  K K+Y  NF 
Sbjct: 114 NPLMGWASTADPLSNM---VLTFSAKEDAVAFAEKHGWSYDVEGRKVPKPKSKSYGANFS 166

[Close]

BLAST of Spg012223 vs. ExPASy Swiss-Prot
Match: Q02375 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Bos taurus OX=9913 GN=NDUFS4 PE=1 SV=1)

HSP 1 Score: 94.0 bits (232), Expect = 1.5e-18
Identity = 49/110 (44.55%), Postives = 65/110 (59.09%), Query Frame = 0


Query: 38  EIGMVSGIPDEHL-TRKVVIYSPARTASQQGSGKVGK----------WENPLMGWTSTGD 97
           +I  ++G+P+EH+ TRK  I+ PAR   Q G     K          WENPLMGW ST D
Sbjct: 60  DITTITGVPEEHIKTRKARIFVPARNNMQSGVNNTKKWKMEFDTRERWENPLMGWASTAD 119

Query: 98  PYANVGDSALSFDSEEAAKAFAEKHGWEYVVKKRHTPLLKVKAYADNFKW 137
           P +N+    L+F ++E A AFAEK+GW Y V++R  P  K K+Y  NF W
Sbjct: 120 PLSNL---VLTFSTKEDAVAFAEKNGWSYDVEERKVPKPKSKSYGANFSW 166

[Close]

BLAST of Spg012223 vs. ExPASy Swiss-Prot
Match: Q66XS7 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Gekko japonicus OX=146911 GN=NDUFS4 PE=2 SV=1)

HSP 1 Score: 93.6 bits (231), Expect = 2.0e-18
Identity = 49/110 (44.55%), Postives = 67/110 (60.91%), Query Frame = 0


Query: 38  EIGMVSGIPDEHL-TRKVVIYSPARTASQQGSGKVGK----------WENPLMGWTSTGD 97
           +I  ++G+P+EH+ TRKV I+ PAR   Q G     K          WENPLMGW+ST D
Sbjct: 60  DITTLTGVPEEHIKTRKVRIFVPARNNMQSGVNNTKKWKMEFDTRERWENPLMGWSSTAD 119

Query: 98  PYANVGDSALSFDSEEAAKAFAEKHGWEYVVKKRHTPLLKVKAYADNFKW 137
           P +N+    L+F ++E A AFAEK+GW + V++R  P  K K+Y  NF W
Sbjct: 120 PLSNL---VLTFSTKEDAVAFAEKNGWSFDVEERKVPKPKSKSYGANFSW 166

[Close]

BLAST of Spg012223 vs. ExPASy Swiss-Prot
Match: Q0MQH0 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Gorilla gorilla gorilla OX=9595 GN=NDUFS4 PE=2 SV=1)

HSP 1 Score: 93.2 bits (230), Expect = 2.6e-18
Identity = 48/110 (43.64%), Postives = 66/110 (60.00%), Query Frame = 0


Query: 38  EIGMVSGIPDEHL-TRKVVIYSPARTASQQGSGKVGK----------WENPLMGWTSTGD 97
           +I  ++G+P+EH+ TRKV I+ PAR   Q G     K          WENPLMGW ST D
Sbjct: 60  DITTLTGVPEEHIKTRKVRIFVPARNNMQSGVNNTKKWKMEFDTRERWENPLMGWASTAD 119

Query: 98  PYANVGDSALSFDSEEAAKAFAEKHGWEYVVKKRHTPLLKVKAYADNFKW 137
           P +N+    L+F ++E A +FAEK+GW Y +++R  P  K K+Y  NF W
Sbjct: 120 PLSNM---VLTFSTKEDAVSFAEKNGWSYDIEERKVPKPKSKSYGANFSW 166

[Close]

BLAST of Spg012223 vs. ExPASy TrEMBL
Match: A0A6J1BSY3 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Momordica charantia OX=3673 GN=LOC111005430 PE=3 SV=1)

HSP 1 Score: 283.9 bits (725), Expect = 3.8e-73
Identity = 138/155 (89.03%), Postives = 143/155 (92.26%), Query Frame = 0


Query: 1   MANSLQRIWNHRYALRGTVAPLCRSFSADSLVEVKPGEIGMVSGIPDEHLTRKVVIYSPA 60
           MANSLQRIWNHR  LRGT+APLCRSFSADSLV+VKPGEIGMVSGIP+EHLTR+VVIYSPA
Sbjct: 1   MANSLQRIWNHRCGLRGTLAPLCRSFSADSLVQVKPGEIGMVSGIPEEHLTRRVVIYSPA 60

Query: 61  RTASQQGSGKVG----------KWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEK 120
           RTASQQGSGKVG          KWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEK
Sbjct: 61  RTASQQGSGKVGKWKINFMSTQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEK 120

Query: 121 HGWEYVVKKRHTPLLKVKAYADNFKWKGPPEAKEA 146
           HGW+YVVKKRHTPLLKVKAYADNFKWKGPPEAKEA
Sbjct: 121 HGWDYVVKKRHTPLLKVKAYADNFKWKGPPEAKEA 155

[Close]

BLAST of Spg012223 vs. ExPASy TrEMBL
Match: A0A6J1HE01 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111463327 PE=3 SV=1)

HSP 1 Score: 275.0 bits (702), Expect = 1.8e-70
Identity = 136/155 (87.74%), Postives = 139/155 (89.68%), Query Frame = 0


Query: 1   MANSLQRIWNHRYALRGTVAPLCRSFSADSLVEVKPGEIGMVSGIPDEHLTRKVVIYSPA 60
           MANSLQRIWN   ALRGT+APLCRSFSADSLVEVKPGEIGMVSGIP+EHLTR+VVIYSPA
Sbjct: 1   MANSLQRIWNRGCALRGTMAPLCRSFSADSLVEVKPGEIGMVSGIPEEHLTRRVVIYSPA 60

Query: 61  RTASQQGSGKVG----------KWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEK 120
           RTASQQGSGKVG          KWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEK
Sbjct: 61  RTASQQGSGKVGKWKINFMSTQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEK 120

Query: 121 HGWEYVVKKRHTPLLKVKAYADNFKWKGPPEAKEA 146
           HGWEYVVKKRHTPLLK KAYADNFKWKG PE KEA
Sbjct: 121 HGWEYVVKKRHTPLLKAKAYADNFKWKGHPEPKEA 155

[Close]

BLAST of Spg012223 vs. ExPASy TrEMBL
Match: A0A6J1KR46 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111496890 PE=3 SV=1)

HSP 1 Score: 275.0 bits (702), Expect = 1.8e-70
Identity = 136/155 (87.74%), Postives = 139/155 (89.68%), Query Frame = 0


Query: 1   MANSLQRIWNHRYALRGTVAPLCRSFSADSLVEVKPGEIGMVSGIPDEHLTRKVVIYSPA 60
           MANSLQRIWN   ALRGT+APLCRSFSADSLVEVKPGEIGMVSGIP+EHLTR+VVIYSPA
Sbjct: 1   MANSLQRIWNRGCALRGTMAPLCRSFSADSLVEVKPGEIGMVSGIPEEHLTRRVVIYSPA 60

Query: 61  RTASQQGSGKVG----------KWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEK 120
           RTASQQGSGKVG          KWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEK
Sbjct: 61  RTASQQGSGKVGKWKINFMSTQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEK 120

Query: 121 HGWEYVVKKRHTPLLKVKAYADNFKWKGPPEAKEA 146
           HGWEYVVKKRHTPLLK KAYADNFKWKG PE KEA
Sbjct: 121 HGWEYVVKKRHTPLLKAKAYADNFKWKGHPEPKEA 155

[Close]

BLAST of Spg012223 vs. ExPASy TrEMBL
Match: A0A6J1IIF4 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111477781 PE=3 SV=1)

HSP 1 Score: 273.5 bits (698), Expect = 5.2e-70
Identity = 134/155 (86.45%), Postives = 141/155 (90.97%), Query Frame = 0


Query: 1   MANSLQRIWNHRYALRGTVAPLCRSFSADSLVEVKPGEIGMVSGIPDEHLTRKVVIYSPA 60
           MANSLQRIW++  ALRGTVAPLCRSFS+DSLV++KPGEIGMVSGIPDEHLTR+VVIYSPA
Sbjct: 1   MANSLQRIWSNSRALRGTVAPLCRSFSSDSLVQLKPGEIGMVSGIPDEHLTRRVVIYSPA 60

Query: 61  RTASQQGSGKVG----------KWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEK 120
           RTASQQGSGKVG          KWENPLMGWTSTGDPYANVGDSALSFDSEEAAK FA+K
Sbjct: 61  RTASQQGSGKVGKWKINFMSTQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKEFADK 120

Query: 121 HGWEYVVKKRHTPLLKVKAYADNFKWKGPPEAKEA 146
           HGWEYVVKKRHTPLLKVKAYADNFKWKG PEAKEA
Sbjct: 121 HGWEYVVKKRHTPLLKVKAYADNFKWKGHPEAKEA 155

[Close]

BLAST of Spg012223 vs. ExPASy TrEMBL
Match: A0A6J1GDQ9 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111453236 PE=3 SV=1)

HSP 1 Score: 272.7 bits (696), Expect = 8.8e-70
Identity = 133/155 (85.81%), Postives = 141/155 (90.97%), Query Frame = 0


Query: 1   MANSLQRIWNHRYALRGTVAPLCRSFSADSLVEVKPGEIGMVSGIPDEHLTRKVVIYSPA 60
           MANSLQRIW++  A+RGTVAPLCRSFS+DSLV++KPGEIGMVSGIPDEHLTR+VVIYSPA
Sbjct: 1   MANSLQRIWSNSRAIRGTVAPLCRSFSSDSLVQLKPGEIGMVSGIPDEHLTRRVVIYSPA 60

Query: 61  RTASQQGSGKVG----------KWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEK 120
           RTASQQGSGKVG          KWENPLMGWTSTGDPYANVGDSALSFDSEEAAK FA+K
Sbjct: 61  RTASQQGSGKVGKWKINFMSTQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKEFADK 120

Query: 121 HGWEYVVKKRHTPLLKVKAYADNFKWKGPPEAKEA 146
           HGWEYVVKKRHTPLLKVKAYADNFKWKG PEAKEA
Sbjct: 121 HGWEYVVKKRHTPLLKVKAYADNFKWKGHPEAKEA 155

[Close]

BLAST of Spg012223 vs. TAIR 10
Match: AT5G67590.1 (NADH-ubiquinone oxidoreductase-related )

HSP 1 Score: 194.5 bits (493), Expect = 5.9e-50
Identity = 94/139 (67.63%), Postives = 113/139 (81.29%), Query Frame = 0


Query: 15  LRGTVAPLCRSFSADSLVE--VKPGEIGMVSGIPDEHLTRKVVIYSPARTASQQGSGKVG 74
           +  T+  + R F+ D++VE   K GEIG VSGIP+EHL+RKV+IYSPARTA+Q GSGK+G
Sbjct: 13  IAATLRRVARPFATDAVVESDYKRGEIGKVSGIPEEHLSRKVIIYSPARTATQSGSGKLG 72

Query: 75  ----------KWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEKHGWEYVVKKRHT 134
                     KWENPLMGWTSTGDPYANVGDSAL+FDSEEAAK+FAE+HGW+Y VKK +T
Sbjct: 73  KWKINFVSTLKWENPLMGWTSTGDPYANVGDSALAFDSEEAAKSFAERHGWDYKVKKPNT 132

Query: 135 PLLKVKAYADNFKWKGPPE 142
           PLLKVK+Y+DNFKWKG P+
Sbjct: 133 PLLKVKSYSDNFKWKGNPQ 151

The following BLAST results are available for this feature:

BLAST of Spg012223 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022132610.1	7.9e-73	89.03	NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial [Momordica ...	[more]
XP_038881462.1	1.5e-71	87.74	NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial [Benincasa ...	[more]
XP_022962977.1	3.7e-70	87.74	NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial-like [Cucur...	[more]
XP_022977437.1	1.1e-69	86.45	NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial-like [Cucur...	[more]
XP_022950018.1	1.8e-69	85.81	NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial-like [Cucur...	[more]

BLAST of Spg012223 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FJW4	8.3e-49	67.63	NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Arabidop...	[more]
Q5XIF3	3.1e-19	43.80	NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Rattus n...	[more]
Q02375	1.5e-18	44.55	NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Bos taur...	[more]
Q66XS7	2.0e-18	44.55	NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Gekko ja...	[more]
Q0MQH0	2.6e-18	43.64	NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Gorilla ...	[more]

BLAST of Spg012223 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BSY3	3.8e-73	89.03	NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Momordic...	[more]
A0A6J1HE01	1.8e-70	87.74	NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Cucurbit...	[more]
A0A6J1KR46	1.8e-70	87.74	NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Cucurbit...	[more]
A0A6J1IIF4	5.2e-70	86.45	NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Cucurbit...	[more]
A0A6J1GDQ9	8.8e-70	85.81	NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Cucurbit...	[more]

BLAST of Spg012223 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G67590.1	5.9e-50	67.63	NADH-ubiquinone oxidoreductase-related	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006885	NADH dehydrogenase ubiquinone Fe-S protein 4, mitochondrial	PFAM	PF04800	ETC_C1_NDUFA4	coord: 53..137 e-value: 1.4E-23 score: 82.9
IPR006885	NADH dehydrogenase ubiquinone Fe-S protein 4, mitochondrial	PANTHER	PTHR12219	NADH-UBIQUINONE OXIDOREDUCTASE	coord: 23..144
IPR038532	NADH dehydrogenase ubiquinone Fe-S protein 4-like superfamily	GENE3D	3.30.160.190	atu1810 like domain	coord: 52..117 e-value: 2.1E-23 score: 84.1
None	No IPR available	PANTHER	PTHR12219:SF23	NADH DEHYDROGENASE [UBIQUINONE] IRON-SULFUR PROTEIN 4, MITOCHONDRIAL-LIKE	coord: 23..144

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg012223.1	Spg012223.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0022900	electron transport chain
cellular_component	GO:0005743	mitochondrial inner membrane
cellular_component	GO:0070469	respirasome
molecular_function	GO:0016651	oxidoreductase activity, acting on NAD(P)H