Spg012164 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg012164
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Auxin-responsive GH3 family protein
Location	scaffold1: 8281182 .. 8283658 (-)
RNA-Seq Expression	Spg012164
Synteny	Spg012164

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAGCATTTATCCACTGCCATATCAGTAGAAGAATTCTTTGCTTTCTTTGACAATGGCCGTTGTTTCGAATTCTTCGCTTCCTCACGCCAAGGCTCTCCATTTCATAGAGGAGACGACTACAAACGCCGACTCGGTTCAGCAGACGGTCTTGGCCGATATTCTGAACCGGAACGCCGATACGGAGTATCTGAACCGGTTCCGGCTCAATGGAGCCACCGACCGAGACACCTTTAAGTCCAAAGTTCCGGTGGTGACTTACGAGGATCTCCAGCCCGACATCCAACGCATTGCTAATGGCGATCGCTCTCCTATTTTCTCTTCTCATCCCATTTCTGAATTTCTTACAAGGTAAAAACTTTTAATTCCAACATATAAGTAATTAAACTAAAATATATCAAGTTCAATTCTTGAGATTAAATTGGGTTTTAATTTGTTTTGTGAATTTGCAGTTCTGGGACATCGGCTGGAGAGAGAAAACTGATGCCTACAATTAAAGAAGAAATGGAACGTCGTCAGCTGCTTTACAGTCTTCTCATGCCCATCATGAACCTGTAAGAATATAATTCACTCTCCATTCAATTTCAATTCTGTCACTTTCTTAATTCAAGACAAGAATTTAATTTTTGACGAATTTACAAAATATTCAGGTACGTGCCGGGGTTGGACAAAGGGAAAGGGCTCTACTTTCTATTCGTGAAGGCCGAAACCAAGACCCCTGGAGGGCTGGTGGCTCGTCCAGTGCTCACCAGCTACTACAAGAGCGACATCTTCAAGACTAGACCCTACGACCCTTTCAATGACTACACCAGCCCAAACGAGGCCATTCTATGCCCCAATTCCTTCCAAAGCATGTACACTCAGATGCTATGTGGCCTCTTGATGCGCGGCCAAGTCCTCCGGGTCGGCGCCGTCTTCGCCTCCGGCCTCCTCCGTGCCATTCACTTTCTCCAGCTCAACTGGAAGCAGTTTGCACACGACATCTCCACCGGAACCCTTAACCCCAAAATCACTGACCCTTCTCTTCGTGAATGCATAATCTCAAAATACTTGACAAACCCAAATCCCGAGCTGGCCGAATTCATTTCCAAGGAGTGCTCCACAGAGGAATGGGAAGGAATCGTCACACGAATCTGGCCAAACACAAAATACCTGGATGTGATCGTGACAGGAGCGATGGCTCAGTACATTCCGACGCTGGAATTTTACAGCGGGGGATTACCCATGGCTTGCACCATGTACGCTTCCTCGGAGTGCTATTTCGGGGTTAATTTGAAGCCAATGTGCAAACCCTCGGAAGTAACCTACACCATCATGCCCAACATGGGCTACTTCGAATTCCTCCCGCACGACACGTCGTCCCCTCCAGCCCTCTCCCGCGACTCCCCGCCTCGACTCGTCGACCTCGCTGACGTGGAAGTCGGGAAAGAGTACGAGCTCGTGATCACAACCTACTCCGGACTGTGCCGCTACCGCGTCGGCGACATCCTACAGGTAACTGGGTTCCACAACGCGGCCCCACAGTTCCGATTCGTGAGGAGAAAGAACGTTTTGTTGAGCATCGATTCCGACAAGACGGATGAGGCCGAGTTGCAAAAGGCCATCGACAAGGCGGCGTTGTTGCTGCGGGAGTTCAATACGAGCGTGGTGGAGTACACGAGCTACGCAGATACCAAAACAATCCCAGGACATTACGTGATTTATTGGGAGCTTCATGTCAAAGACGCTGCAAATCCCCCGCCGGGGGAGGTTCTAAACCAGTGCTGCTTGGCCATGGAAGAGAGCCTGAACTCGGTGTACCGCCAATGTCGAGTGGCGGATAACTCGATCGGGCCGCTGGAGATCAGGGTGGTGAAAAACGGGACGTTTGAAGAGCTAATGGACTACGCGATCTCAAGGGGCGCGTCAATCAATCAGTACAAAGCGCCACGCTGCGTAAACTTCACACCCATTGTCGAACTCCTTGACTCTAGGGTAACTTCGGCCCACTTCAGCCCATCGAAGCCACAATGGACACCGGAACGACGCCGTTGAGAGCCGAGAAGAACTCAACCAACAAGTTTTAACAAATTATTAAATAATAATATTATTATTATTTTTTTATAAAAAAATTATATGTTGTAGACAGTTGAGTTTAGTGGATTATGCATATGGCTTTTATAAGTGTCTTTGATTTGTGGGGTTTTTATAAGTTGATGTAGTGTGTCTGTGTTACACCCCTAAGTCTGATTTTCCCCCGTGACAGTCTGTGTAACGAGGTTGTGAGTAATGTCTAAATTAATATGAGATTCATGATGTAATTGTTGTTTTTGTTGGTCTATTTGCATGTTTTGTCATTGGCTTTCTTTCCTTTCCTTTTTGAGATTAGAGAAAGAGGAAAGAAAGGAAAAAGGAAAAGGTAAATAATTACTTTATGAGATAGATTTGATGTACTGTTGTTTCTTTTCAAGACAATCACACAAAGACTACATCATTTTTCC

mRNA sequence

ATGGCCGTTGTTTCGAATTCTTCGCTTCCTCACGCCAAGGCTCTCCATTTCATAGAGGAGACGACTACAAACGCCGACTCGGTTCAGCAGACGGTCTTGGCCGATATTCTGAACCGGAACGCCGATACGGAGTATCTGAACCGGTTCCGGCTCAATGGAGCCACCGACCGAGACACCTTTAAGTCCAAAGTTCCGGTGGTGACTTACGAGGATCTCCAGCCCGACATCCAACGCATTGCTAATGGCGATCGCTCTCCTATTTTCTCTTCTCATCCCATTTCTGAATTTCTTACAAGTTCTGGGACATCGGCTGGAGAGAGAAAACTGATGCCTACAATTAAAGAAGAAATGGAACGTCGTCAGCTGCTTTACAGTCTTCTCATGCCCATCATGAACCTGTACGTGCCGGGGTTGGACAAAGGGAAAGGGCTCTACTTTCTATTCGTGAAGGCCGAAACCAAGACCCCTGGAGGGCTGGTGGCTCGTCCAGTGCTCACCAGCTACTACAAGAGCGACATCTTCAAGACTAGACCCTACGACCCTTTCAATGACTACACCAGCCCAAACGAGGCCATTCTATGCCCCAATTCCTTCCAAAGCATGTACACTCAGATGCTATGTGGCCTCTTGATGCGCGGCCAAGTCCTCCGGGTCGGCGCCGTCTTCGCCTCCGGCCTCCTCCGTGCCATTCACTTTCTCCAGCTCAACTGGAAGCAGTTTGCACACGACATCTCCACCGGAACCCTTAACCCCAAAATCACTGACCCTTCTCTTCGTGAATGCATAATCTCAAAATACTTGACAAACCCAAATCCCGAGCTGGCCGAATTCATTTCCAAGGAGTGCTCCACAGAGGAATGGGAAGGAATCGTCACACGAATCTGGCCAAACACAAAATACCTGGATGTGATCGTGACAGGAGCGATGGCTCAGTACATTCCGACGCTGGAATTTTACAGCGGGGGATTACCCATGGCTTGCACCATGTACGCTTCCTCGGAGTGCTATTTCGGGGTTAATTTGAAGCCAATGTGCAAACCCTCGGAAGTAACCTACACCATCATGCCCAACATGGGCTACTTCGAATTCCTCCCGCACGACACGTCGTCCCCTCCAGCCCTCTCCCGCGACTCCCCGCCTCGACTCGTCGACCTCGCTGACGTGGAAGTCGGGAAAGAGTACGAGCTCGTGATCACAACCTACTCCGGACTGTGCCGCTACCGCGTCGGCGACATCCTACAGGTAACTGGGTTCCACAACGCGGCCCCACAGTTCCGATTCGTGAGGAGAAAGAACGTTTTGTTGAGCATCGATTCCGACAAGACGGATGAGGCCGAGTTGCAAAAGGCCATCGACAAGGCGGCGTTGTTGCTGCGGGAGTTCAATACGAGCGTGGTGGAGTACACGAGCTACGCAGATACCAAAACAATCCCAGGACATTACGTGATTTATTGGGAGCTTCATGTCAAAGACGCTGCAAATCCCCCGCCGGGGGAGGTTCTAAACCAGTGCTGCTTGGCCATGGAAGAGAGCCTGAACTCGGTGTACCGCCAATGTCGAGTGGCGGATAACTCGATCGGGCCGCTGGAGATCAGGGTGGTGAAAAACGGGACGTTTGAAGAGCTAATGGACTACGCGATCTCAAGGGGCGCGTCAATCAATCAGTACAAAGCGCCACGCTGCGTAAACTTCACACCCATTGTCGAACTCCTTGACTCTAGGGTAACTTCGGCCCACTTCAGCCCATCGAAGCCACAATGGACACCGGAACGACGCCGTTGA

Coding sequence (CDS)

ATGGCCGTTGTTTCGAATTCTTCGCTTCCTCACGCCAAGGCTCTCCATTTCATAGAGGAGACGACTACAAACGCCGACTCGGTTCAGCAGACGGTCTTGGCCGATATTCTGAACCGGAACGCCGATACGGAGTATCTGAACCGGTTCCGGCTCAATGGAGCCACCGACCGAGACACCTTTAAGTCCAAAGTTCCGGTGGTGACTTACGAGGATCTCCAGCCCGACATCCAACGCATTGCTAATGGCGATCGCTCTCCTATTTTCTCTTCTCATCCCATTTCTGAATTTCTTACAAGTTCTGGGACATCGGCTGGAGAGAGAAAACTGATGCCTACAATTAAAGAAGAAATGGAACGTCGTCAGCTGCTTTACAGTCTTCTCATGCCCATCATGAACCTGTACGTGCCGGGGTTGGACAAAGGGAAAGGGCTCTACTTTCTATTCGTGAAGGCCGAAACCAAGACCCCTGGAGGGCTGGTGGCTCGTCCAGTGCTCACCAGCTACTACAAGAGCGACATCTTCAAGACTAGACCCTACGACCCTTTCAATGACTACACCAGCCCAAACGAGGCCATTCTATGCCCCAATTCCTTCCAAAGCATGTACACTCAGATGCTATGTGGCCTCTTGATGCGCGGCCAAGTCCTCCGGGTCGGCGCCGTCTTCGCCTCCGGCCTCCTCCGTGCCATTCACTTTCTCCAGCTCAACTGGAAGCAGTTTGCACACGACATCTCCACCGGAACCCTTAACCCCAAAATCACTGACCCTTCTCTTCGTGAATGCATAATCTCAAAATACTTGACAAACCCAAATCCCGAGCTGGCCGAATTCATTTCCAAGGAGTGCTCCACAGAGGAATGGGAAGGAATCGTCACACGAATCTGGCCAAACACAAAATACCTGGATGTGATCGTGACAGGAGCGATGGCTCAGTACATTCCGACGCTGGAATTTTACAGCGGGGGATTACCCATGGCTTGCACCATGTACGCTTCCTCGGAGTGCTATTTCGGGGTTAATTTGAAGCCAATGTGCAAACCCTCGGAAGTAACCTACACCATCATGCCCAACATGGGCTACTTCGAATTCCTCCCGCACGACACGTCGTCCCCTCCAGCCCTCTCCCGCGACTCCCCGCCTCGACTCGTCGACCTCGCTGACGTGGAAGTCGGGAAAGAGTACGAGCTCGTGATCACAACCTACTCCGGACTGTGCCGCTACCGCGTCGGCGACATCCTACAGGTAACTGGGTTCCACAACGCGGCCCCACAGTTCCGATTCGTGAGGAGAAAGAACGTTTTGTTGAGCATCGATTCCGACAAGACGGATGAGGCCGAGTTGCAAAAGGCCATCGACAAGGCGGCGTTGTTGCTGCGGGAGTTCAATACGAGCGTGGTGGAGTACACGAGCTACGCAGATACCAAAACAATCCCAGGACATTACGTGATTTATTGGGAGCTTCATGTCAAAGACGCTGCAAATCCCCCGCCGGGGGAGGTTCTAAACCAGTGCTGCTTGGCCATGGAAGAGAGCCTGAACTCGGTGTACCGCCAATGTCGAGTGGCGGATAACTCGATCGGGCCGCTGGAGATCAGGGTGGTGAAAAACGGGACGTTTGAAGAGCTAATGGACTACGCGATCTCAAGGGGCGCGTCAATCAATCAGTACAAAGCGCCACGCTGCGTAAACTTCACACCCATTGTCGAACTCCTTGACTCTAGGGTAACTTCGGCCCACTTCAGCCCATCGAAGCCACAATGGACACCGGAACGACGCCGTTGA

Protein sequence

MAVVSNSSLPHAKALHFIEETTTNADSVQQTVLADILNRNADTEYLNRFRLNGATDRDTFKSKVPVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKEEMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSDIFKTRPYDPFNDYTSPNEAILCPNSFQSMYTQMLCGLLMRGQVLRVGAVFASGLLRAIHFLQLNWKQFAHDISTGTLNPKITDPSLRECIISKYLTNPNPELAEFISKECSTEEWEGIVTRIWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLKPMCKPSEVTYTIMPNMGYFEFLPHDTSSPPALSRDSPPRLVDLADVEVGKEYELVITTYSGLCRYRVGDILQVTGFHNAAPQFRFVRRKNVLLSIDSDKTDEAELQKAIDKAALLLREFNTSVVEYTSYADTKTIPGHYVIYWELHVKDAANPPPGEVLNQCCLAMEESLNSVYRQCRVADNSIGPLEIRVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVTSAHFSPSKPQWTPERRR

Homology

[Close]

BLAST of Spg012164 vs. NCBI nr
Match: KAG6604151.1 (putative indole-3-acetic acid-amido synthetase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1099.3 bits (2842), Expect = 0.0e+00
Identity = 543/603 (90.05%), Postives = 566/603 (93.86%), Query Frame = 0


Query: 1   MAVVSNSSLP--------HAKALHFIEETTTNADSVQQTVLADILNRNADTEYLNRFRLN 60
           MAV S  S P         AKAL FIEE TTN D+VQQ VLA+IL+RNA+TEY+NRFRLN
Sbjct: 1   MAVDSQLSSPLPPPPSDKDAKALRFIEEVTTNTDAVQQRVLAEILSRNANTEYVNRFRLN 60

Query: 61  GATDRDTFKSKVPVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPT 120
           GATDRDT+KSK+PVVTYEDLQPDIQRIANGDRSPIFSS PISEFLTSSGTSAGERKLMPT
Sbjct: 61  GATDRDTYKSKIPVVTYEDLQPDIQRIANGDRSPIFSSTPISEFLTSSGTSAGERKLMPT 120

Query: 121 IKEEMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSD 180
           I+EEMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSD
Sbjct: 121 IQEEMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSD 180

Query: 181 IFKTRPYDPFNDYTSPNEAILCPNSFQSMYTQMLCGLLMRGQVLRVGAVFASGLLRAIHF 240
           IFKTRPYDPFNDYTSPNEA+LC +SFQSMYTQMLCGLLMR QVLRVGAVFASGLLRAI F
Sbjct: 181 IFKTRPYDPFNDYTSPNEAVLCADSFQSMYTQMLCGLLMREQVLRVGAVFASGLLRAIRF 240

Query: 241 LQLNWKQFAHDISTGTLNPKITDPSLRECIISKYLTNPNPELAEFISKECSTEEWEGIVT 300
           LQLNWKQ AHDISTGTLNPKITDP LRECI+SKYLTNPNPE+AEFISKECSTEEWEGI+T
Sbjct: 241 LQLNWKQLAHDISTGTLNPKITDPCLRECIVSKYLTNPNPEVAEFISKECSTEEWEGIIT 300

Query: 301 RIWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLKPMCKPSEVTY 360
           RIWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLKPMCKPSEVTY
Sbjct: 301 RIWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLKPMCKPSEVTY 360

Query: 361 TIMPNMGYFEFLPHDTSSPPALSRDSPPRLVDLADVEVGKEYELVITTYSGLCRYRVGDI 420
           TIMPNMGYFEFLPHDTSSPP LSRDSPPRLVDLADVEVGKEYELVITTY+GL RYRVGD+
Sbjct: 361 TIMPNMGYFEFLPHDTSSPPVLSRDSPPRLVDLADVEVGKEYELVITTYAGLRRYRVGDV 420

Query: 421 LQVTGFHNAAPQFRFVRRKNVLLSIDSDKTDEAELQKAIDKAALLLREFNTSVVEYTSYA 480
           L+VTGFHNAAPQFRFVRRKNVLLSID+DKTDEAELQKA++ A+ LLRE NTSVVEYTSYA
Sbjct: 421 LEVTGFHNAAPQFRFVRRKNVLLSIDADKTDEAELQKAVENASSLLRELNTSVVEYTSYA 480

Query: 481 DTKTIPGHYVIYWELHVKDAA--NPPPGEVLNQCCLAMEESLNSVYRQCRVADNSIGPLE 540
           DTKTIPGHYVIYWEL VKD A  +PPP E+LNQCCLA+EESLNSVYRQ RVADNSIGPLE
Sbjct: 481 DTKTIPGHYVIYWELLVKDEAANSPPPAEILNQCCLAIEESLNSVYRQGRVADNSIGPLE 540

Query: 541 IRVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVTSAHFSPSKPQWTPE 594
           IRVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRV S HFSPSKP WTPE
Sbjct: 541 IRVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVASVHFSPSKPHWTPE 600

[Close]

BLAST of Spg012164 vs. NCBI nr
Match: XP_023543141.1 (probable indole-3-acetic acid-amido synthetase GH3.1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1099.3 bits (2842), Expect = 0.0e+00
Identity = 543/603 (90.05%), Postives = 566/603 (93.86%), Query Frame = 0


Query: 1   MAVVSNSSLP--------HAKALHFIEETTTNADSVQQTVLADILNRNADTEYLNRFRLN 60
           MAV S  S P         AKAL FI+E TTN D+VQQ VLA+IL+RNA+TEY+NRFRLN
Sbjct: 1   MAVDSQLSSPLPPPPSDKDAKALRFIQEVTTNTDAVQQRVLAEILSRNANTEYVNRFRLN 60

Query: 61  GATDRDTFKSKVPVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPT 120
           GATDRDT+KSK+PVVTYEDLQPDIQRIANGDRSPIFSS PISEFLTSSGTSAGERKLMPT
Sbjct: 61  GATDRDTYKSKIPVVTYEDLQPDIQRIANGDRSPIFSSTPISEFLTSSGTSAGERKLMPT 120

Query: 121 IKEEMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSD 180
           I+EEMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSD
Sbjct: 121 IQEEMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSD 180

Query: 181 IFKTRPYDPFNDYTSPNEAILCPNSFQSMYTQMLCGLLMRGQVLRVGAVFASGLLRAIHF 240
           IFKTRPYDPFNDYTSPNEA+LC +SFQSMYTQMLCGLLMR QVLRVGAVFASGLLRAI F
Sbjct: 181 IFKTRPYDPFNDYTSPNEAVLCADSFQSMYTQMLCGLLMREQVLRVGAVFASGLLRAIRF 240

Query: 241 LQLNWKQFAHDISTGTLNPKITDPSLRECIISKYLTNPNPELAEFISKECSTEEWEGIVT 300
           LQLNWKQ AHDISTGTLNPKITDP LRECI+SKYLTNPNPE+AEFISKECSTEEWEGI+T
Sbjct: 241 LQLNWKQLAHDISTGTLNPKITDPCLRECIVSKYLTNPNPEVAEFISKECSTEEWEGIIT 300

Query: 301 RIWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLKPMCKPSEVTY 360
           RIWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLKPMCKPSEVTY
Sbjct: 301 RIWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLKPMCKPSEVTY 360

Query: 361 TIMPNMGYFEFLPHDTSSPPALSRDSPPRLVDLADVEVGKEYELVITTYSGLCRYRVGDI 420
           TIMPNMGYFEFLPHDTSSPP LSRDSPPRLVDLADVEVGKEYELVITTY+GL RYRVGD+
Sbjct: 361 TIMPNMGYFEFLPHDTSSPPVLSRDSPPRLVDLADVEVGKEYELVITTYAGLRRYRVGDV 420

Query: 421 LQVTGFHNAAPQFRFVRRKNVLLSIDSDKTDEAELQKAIDKAALLLREFNTSVVEYTSYA 480
           L+VTGFHNAAPQFRFVRRKNVLLSIDSDKTDEAELQKA++ A+ LLRE NTSVVEYTSYA
Sbjct: 421 LEVTGFHNAAPQFRFVRRKNVLLSIDSDKTDEAELQKAVENASSLLRELNTSVVEYTSYA 480

Query: 481 DTKTIPGHYVIYWELHVKDAA--NPPPGEVLNQCCLAMEESLNSVYRQCRVADNSIGPLE 540
           DTKTIPGHYVIYWEL VKD A  +PPP E+LNQCCLA+EESLNSVYRQ RVADNSIGPLE
Sbjct: 481 DTKTIPGHYVIYWELLVKDEAANSPPPAEILNQCCLAIEESLNSVYRQGRVADNSIGPLE 540

Query: 541 IRVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVTSAHFSPSKPQWTPE 594
           IRVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRV S HFSPSKP WTPE
Sbjct: 541 IRVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVASVHFSPSKPHWTPE 600

[Close]

BLAST of Spg012164 vs. NCBI nr
Match: KAG7034313.1 (putative indole-3-acetic acid-amido synthetase GH3.1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1098.6 bits (2840), Expect = 0.0e+00
Identity = 536/584 (91.78%), Postives = 560/584 (95.89%), Query Frame = 0


Query: 12   AKALHFIEETTTNADSVQQTVLADILNRNADTEYLNRFRLNGATDRDTFKSKVPVVTYED 71
            AKAL FIEE TTN D+VQQ VLA+IL+RNA+TEY+NRFRLNGATDRDT+KSK+PVVTYED
Sbjct: 608  AKALRFIEEVTTNTDAVQQRVLAEILSRNANTEYVNRFRLNGATDRDTYKSKIPVVTYED 667

Query: 72   LQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKEEMERRQLLYSLLMPIM 131
            LQPDIQRIANGDRSPIFSS PISEFLTSSGTSAGERKLMPTI+EEMERRQLLYSLLMPIM
Sbjct: 668  LQPDIQRIANGDRSPIFSSTPISEFLTSSGTSAGERKLMPTIQEEMERRQLLYSLLMPIM 727

Query: 132  NLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSDIFKTRPYDPFNDYTSPNEA 191
            NLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSDIFKTRPYDPFNDYTSPNEA
Sbjct: 728  NLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSDIFKTRPYDPFNDYTSPNEA 787

Query: 192  ILCPNSFQSMYTQMLCGLLMRGQVLRVGAVFASGLLRAIHFLQLNWKQFAHDISTGTLNP 251
            +LC +SFQSMYTQMLCGLLMR QVLRVGAVFASGLLRAI FLQLNWKQ AHDISTGTLNP
Sbjct: 788  VLCADSFQSMYTQMLCGLLMREQVLRVGAVFASGLLRAIRFLQLNWKQLAHDISTGTLNP 847

Query: 252  KITDPSLRECIISKYLTNPNPELAEFISKECSTEEWEGIVTRIWPNTKYLDVIVTGAMAQ 311
            KITDP LRECI++KYLTNPNPE+AEFISKECSTEEWEGI+TRIWPNTKYLDVIVTGAMAQ
Sbjct: 848  KITDPCLRECIVTKYLTNPNPEVAEFISKECSTEEWEGIITRIWPNTKYLDVIVTGAMAQ 907

Query: 312  YIPTLEFYSGGLPMACTMYASSECYFGVNLKPMCKPSEVTYTIMPNMGYFEFLPHDTSSP 371
            YIPTLEFYSGGLPMACTMYASSECYFGVNLKPMCKPSEVTYTIMPNMGYFEFLPHDTSSP
Sbjct: 908  YIPTLEFYSGGLPMACTMYASSECYFGVNLKPMCKPSEVTYTIMPNMGYFEFLPHDTSSP 967

Query: 372  PALSRDSPPRLVDLADVEVGKEYELVITTYSGLCRYRVGDILQVTGFHNAAPQFRFVRRK 431
            P LSRDSPPRLVDLADVEVGKEYELVITTY+GL RYRVGD+L+VTGFHNAAPQFRFVRRK
Sbjct: 968  PVLSRDSPPRLVDLADVEVGKEYELVITTYAGLRRYRVGDVLEVTGFHNAAPQFRFVRRK 1027

Query: 432  NVLLSIDSDKTDEAELQKAIDKAALLLREFNTSVVEYTSYADTKTIPGHYVIYWELHVKD 491
            NVLLSID+DKTDEAELQKA++ A+ LLRE NTSVVEYTSYADTKTIPGHYVIYWEL VKD
Sbjct: 1028 NVLLSIDADKTDEAELQKAVENASSLLRELNTSVVEYTSYADTKTIPGHYVIYWELLVKD 1087

Query: 492  AA--NPPPGEVLNQCCLAMEESLNSVYRQCRVADNSIGPLEIRVVKNGTFEELMDYAISR 551
             A  +PPP E+LNQCCLA+EESLNSVYRQ RVADNSIGPLEIRVVKNGTFEELMDYAISR
Sbjct: 1088 EAANSPPPAEILNQCCLAIEESLNSVYRQGRVADNSIGPLEIRVVKNGTFEELMDYAISR 1147

Query: 552  GASINQYKAPRCVNFTPIVELLDSRVTSAHFSPSKPQWTPERRR 594
            GASINQYKAPRCVNFTPIVELLDSRV S HFSPSKP WTPERRR
Sbjct: 1148 GASINQYKAPRCVNFTPIVELLDSRVASVHFSPSKPHWTPERRR 1191

[Close]

BLAST of Spg012164 vs. NCBI nr
Match: XP_022978925.1 (probable indole-3-acetic acid-amido synthetase GH3.1 [Cucurbita maxima])

HSP 1 Score: 1097.0 bits (2836), Expect = 0.0e+00
Identity = 542/603 (89.88%), Postives = 566/603 (93.86%), Query Frame = 0


Query: 1   MAVVSNSSLP--------HAKALHFIEETTTNADSVQQTVLADILNRNADTEYLNRFRLN 60
           MAV S  S P         AKAL FIEE TTNAD+VQQ VLA+IL+RNA+TEY+NRFRLN
Sbjct: 1   MAVGSQLSSPLPPPPSDRDAKALRFIEEVTTNADAVQQRVLAEILSRNANTEYVNRFRLN 60

Query: 61  GATDRDTFKSKVPVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPT 120
           GATDRDT+KSK+PVVTYEDLQPDIQRIANGDRSPIFSS PISEFLTSSGTSAGERKLMPT
Sbjct: 61  GATDRDTYKSKIPVVTYEDLQPDIQRIANGDRSPIFSSTPISEFLTSSGTSAGERKLMPT 120

Query: 121 IKEEMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSD 180
           I+EEMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSD
Sbjct: 121 IQEEMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSD 180

Query: 181 IFKTRPYDPFNDYTSPNEAILCPNSFQSMYTQMLCGLLMRGQVLRVGAVFASGLLRAIHF 240
           IFKTRPYDPFNDYTSPNEA+LC +SFQSMYTQMLCGLLMR QVLRVGAVFASGLLRAI F
Sbjct: 181 IFKTRPYDPFNDYTSPNEAVLCADSFQSMYTQMLCGLLMREQVLRVGAVFASGLLRAIRF 240

Query: 241 LQLNWKQFAHDISTGTLNPKITDPSLRECIISKYLTNPNPELAEFISKECSTEEWEGIVT 300
           LQLNWKQ AHDISTGTLNPKITDP LRECI+SKYLTNPNPE+AEFISKECSTEEWEGI+T
Sbjct: 241 LQLNWKQLAHDISTGTLNPKITDPCLRECIVSKYLTNPNPEVAEFISKECSTEEWEGIIT 300

Query: 301 RIWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLKPMCKPSEVTY 360
           RIWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLKPMCKPSEVTY
Sbjct: 301 RIWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLKPMCKPSEVTY 360

Query: 361 TIMPNMGYFEFLPHDTSSPPALSRDSPPRLVDLADVEVGKEYELVITTYSGLCRYRVGDI 420
           TIMPNMGYFEFLPH TSSPP LSRDSPPRLVDLADVEVGKEYELVITTY+GL RYRVGD+
Sbjct: 361 TIMPNMGYFEFLPHHTSSPPVLSRDSPPRLVDLADVEVGKEYELVITTYAGLRRYRVGDV 420

Query: 421 LQVTGFHNAAPQFRFVRRKNVLLSIDSDKTDEAELQKAIDKAALLLREFNTSVVEYTSYA 480
           L+VTGFHNAAPQFRFVRRKNVLLSID+DKTDEAELQKA++ A+ LLRE NTSVVEYTSYA
Sbjct: 421 LEVTGFHNAAPQFRFVRRKNVLLSIDADKTDEAELQKAVENASSLLRELNTSVVEYTSYA 480

Query: 481 DTKTIPGHYVIYWELHVKDAA--NPPPGEVLNQCCLAMEESLNSVYRQCRVADNSIGPLE 540
           DTKTIPGHYVIYWEL VKD A  +PPP E+LNQCCLA+EESLNSVYRQ RVADNSIGPLE
Sbjct: 481 DTKTIPGHYVIYWELLVKDEAANSPPPAEILNQCCLAIEESLNSVYRQGRVADNSIGPLE 540

Query: 541 IRVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVTSAHFSPSKPQWTPE 594
           IRVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRV S HFSP+KP WTPE
Sbjct: 541 IRVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVVSVHFSPTKPHWTPE 600

[Close]

BLAST of Spg012164 vs. NCBI nr
Match: XP_022950930.1 (probable indole-3-acetic acid-amido synthetase GH3.1 [Cucurbita moschata])

HSP 1 Score: 1096.3 bits (2834), Expect = 0.0e+00
Identity = 542/603 (89.88%), Postives = 565/603 (93.70%), Query Frame = 0


Query: 1   MAVVSNSSLP--------HAKALHFIEETTTNADSVQQTVLADILNRNADTEYLNRFRLN 60
           MAV S  S P         AKAL FIEE TTN D+VQQ VLA+IL+RNA+TEY+NRFRLN
Sbjct: 1   MAVDSQLSSPLPPPPSDKDAKALRFIEEVTTNTDAVQQRVLAEILSRNANTEYVNRFRLN 60

Query: 61  GATDRDTFKSKVPVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPT 120
           GATDRDT+KSK+PVVTYEDLQPDIQRIANGDRSPIFSS PISEFLTSSGTSAGERKLMPT
Sbjct: 61  GATDRDTYKSKIPVVTYEDLQPDIQRIANGDRSPIFSSTPISEFLTSSGTSAGERKLMPT 120

Query: 121 IKEEMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSD 180
           I+EEMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSD
Sbjct: 121 IQEEMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSD 180

Query: 181 IFKTRPYDPFNDYTSPNEAILCPNSFQSMYTQMLCGLLMRGQVLRVGAVFASGLLRAIHF 240
           IFKTRPYDPFNDYTSPNEA+LC +SFQSMYTQMLCGLLMR QVLRVGAVFASGLLRAI F
Sbjct: 181 IFKTRPYDPFNDYTSPNEAVLCADSFQSMYTQMLCGLLMREQVLRVGAVFASGLLRAIRF 240

Query: 241 LQLNWKQFAHDISTGTLNPKITDPSLRECIISKYLTNPNPELAEFISKECSTEEWEGIVT 300
           LQLNWKQ AHDISTGTLNPKITDP LRECI+SKYLTN NPE+AEFISKECSTEEWEGI+T
Sbjct: 241 LQLNWKQLAHDISTGTLNPKITDPCLRECIVSKYLTNTNPEVAEFISKECSTEEWEGIIT 300

Query: 301 RIWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLKPMCKPSEVTY 360
           RIWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLKPMCKPSEVTY
Sbjct: 301 RIWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLKPMCKPSEVTY 360

Query: 361 TIMPNMGYFEFLPHDTSSPPALSRDSPPRLVDLADVEVGKEYELVITTYSGLCRYRVGDI 420
           TIMPNMGYFEFLPHDTSSPP LSRDSPPRLVDLADVEVGKEYELVITTY+GL RYRVGD+
Sbjct: 361 TIMPNMGYFEFLPHDTSSPPVLSRDSPPRLVDLADVEVGKEYELVITTYAGLRRYRVGDV 420

Query: 421 LQVTGFHNAAPQFRFVRRKNVLLSIDSDKTDEAELQKAIDKAALLLREFNTSVVEYTSYA 480
           L+VTGFHNAAPQFRFVRRKNVLLSID+DKTDEAELQKA++ A+ LLRE NTSVVEYTSYA
Sbjct: 421 LEVTGFHNAAPQFRFVRRKNVLLSIDADKTDEAELQKAVENASSLLRELNTSVVEYTSYA 480

Query: 481 DTKTIPGHYVIYWELHVKDAA--NPPPGEVLNQCCLAMEESLNSVYRQCRVADNSIGPLE 540
           DTKTIPGHYVIYWEL VKD A  +PPP E+LNQCCLA+EESLNSVYRQ RVADNSIGPLE
Sbjct: 481 DTKTIPGHYVIYWELLVKDEAANSPPPAEILNQCCLAIEESLNSVYRQGRVADNSIGPLE 540

Query: 541 IRVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVTSAHFSPSKPQWTPE 594
           IRVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRV S HFSPSKP WTPE
Sbjct: 541 IRVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVASVHFSPSKPHWTPE 600

[Close]

BLAST of Spg012164 vs. ExPASy Swiss-Prot
Match: O82333 (Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Arabidopsis thaliana OX=3702 GN=GH3.1 PE=2 SV=1)

HSP 1 Score: 928.7 bits (2399), Expect = 3.3e-269
Identity = 458/602 (76.08%), Postives = 514/602 (85.38%), Query Frame = 0


Query: 1   MAVVSNSSLP--------HAKALHFIEETTTNADSVQQTVLADILNRNADTEYLNRFRLN 60
           MAV SN S P         AKAL FIEE T NAD+VQ+ +LA+IL RNADTEYL RF L 
Sbjct: 1   MAVDSNLSSPLGPPACEKDAKALRFIEEMTRNADTVQENLLAEILARNADTEYLRRFNLC 60

Query: 61  GATDRDTFKSKVPVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPT 120
           GATDRDTFK+K+PV+TYEDLQP+IQRIA+GDRSPI S+HPISEFLTSSGTSAGERKLMPT
Sbjct: 61  GATDRDTFKTKIPVITYEDLQPEIQRIADGDRSPILSAHPISEFLTSSGTSAGERKLMPT 120

Query: 121 IKEEMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSD 180
           IKEE++RRQLLYSLLMP+MNLYVPGLDKGKG+YFLFVK+ETKTPGGL ARPVLTSYYKS+
Sbjct: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGMYFLFVKSETKTPGGLPARPVLTSYYKSE 180

Query: 181 IFKTRPYDPFNDYTSPNEAILCPNSFQSMYTQMLCGLLMRGQVLRVGAVFASGLLRAIHF 240
            F++RPYDP+N YTSPNEAILCP+SFQSMYTQMLCGLL R  VLRVGAVFASGLLRAI F
Sbjct: 181 HFRSRPYDPYNVYTSPNEAILCPDSFQSMYTQMLCGLLDRLSVLRVGAVFASGLLRAIRF 240

Query: 241 LQLNWKQFAHDISTGTLNPKITDPSLRECIISKYLTNPNPELAEFISKECSTEEWEGIVT 300
           LQL+W +FAHDI  G L+ +ITDPS+R+C+    +  P+P LAEFI +EC ++ WE I+T
Sbjct: 241 LQLHWSRFAHDIELGCLDSEITDPSIRQCMSG--ILKPDPVLAEFIRRECKSDNWEKIIT 300

Query: 301 RIWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLKPMCKPSEVTY 360
           RIWPNTKYLDVIVTGAMAQYIPTLE+YSGGLPMACTMYASSECYFG+NL PM KPSEV+Y
Sbjct: 301 RIWPNTKYLDVIVTGAMAQYIPTLEYYSGGLPMACTMYASSECYFGLNLNPMSKPSEVSY 360

Query: 361 TIMPNMGYFEFLPHDTSSPPALSRDSPPRLVDLADVEVGKEYELVITTYSGLCRYRVGDI 420
           TIMPNM YFEF+P   +           + V+L DV +GKEYELV+TTY+GLCRYRVGDI
Sbjct: 361 TIMPNMAYFEFIPLGGT-----------KAVELVDVNIGKEYELVVTTYAGLCRYRVGDI 420

Query: 421 LQVTGFHNAAPQFRFVRRKNVLLSIDSDKTDEAELQKAIDKAALLL-REFNTSVVEYTSY 480
           L+VTGFHN+APQF FVRRKNVLLSIDSDKTDE+ELQKA++ A+ +L  E  + V EYTSY
Sbjct: 421 LRVTGFHNSAPQFHFVRRKNVLLSIDSDKTDESELQKAVENASSILHEECGSRVAEYTSY 480

Query: 481 ADTKTIPGHYVIYWELHVKDAANPPPGEVLNQCCLAMEESLNSVYRQCRVADNSIGPLEI 540
           ADT TIPGHYV+YWEL V+D A  P  E L +CCL MEESLNSVYRQ RVADNS+GPLEI
Sbjct: 481 ADTSTIPGHYVLYWELLVRDGARQPSHETLTRCCLGMEESLNSVYRQSRVADNSVGPLEI 540

Query: 541 RVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVTSAHFSPSKPQWTPER 594
           RVV+NGTFEELMDYAISRGASINQYK PRCVNFTPIVELLDSRV SAHFSPS P WTPER
Sbjct: 541 RVVRNGTFEELMDYAISRGASINQYKVPRCVNFTPIVELLDSRVVSAHFSPSLPHWTPER 589

[Close]

BLAST of Spg012164 vs. ExPASy Swiss-Prot
Match: O22190 (Indole-3-acetic acid-amido synthetase GH3.3 OS=Arabidopsis thaliana OX=3702 GN=GH3.3 PE=1 SV=1)

HSP 1 Score: 905.2 bits (2338), Expect = 3.9e-262
Identity = 451/592 (76.18%), Postives = 508/592 (85.81%), Query Frame = 0


Query: 3   VVSNSSLPHAKALHFIEETTTNADSVQQTVLADILNRNADTEYLNRFRLNGATDRDTFKS 62
           ++ + S    KAL FIEE T N D VQ+ V+ +IL+RN+DTEYL RF L G TDR TFK+
Sbjct: 11  MMHSPSTKDVKALRFIEEMTRNVDFVQKKVIREILSRNSDTEYLKRFGLKGFTDRKTFKT 70

Query: 63  KVPVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKEEMERRQL 122
           KVPVV Y+DL+P+IQRIANGDRS I SS+PI+EFLTSSGTSAGERKLMPTI E+M+RRQL
Sbjct: 71  KVPVVIYDDLKPEIQRIANGDRSMILSSYPITEFLTSSGTSAGERKLMPTIDEDMDRRQL 130

Query: 123 LYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSDIFKTRPYDPF 182
           LYSLLMP+MNLYVPGLDKGK LYFLFVK E+KTPGGL ARPVLTSYYKS+ FK RP DP+
Sbjct: 131 LYSLLMPVMNLYVPGLDKGKALYFLFVKTESKTPGGLPARPVLTSYYKSEQFKRRPNDPY 190

Query: 183 NDYTSPNEAILCPNSFQSMYTQMLCGLLMRGQVLRVGAVFASGLLRAIHFLQLNWKQFAH 242
           N YTSPNEAILCP+S QSMYTQMLCGLLMR +VLR+GAVFASGLLRAI FLQ NWK+ A 
Sbjct: 191 NVYTSPNEAILCPDSSQSMYTQMLCGLLMRHEVLRLGAVFASGLLRAIGFLQTNWKELAD 250

Query: 243 DISTGTLNPKITDPSLRECIISKYLTNPNPELAEFISKECSTE-EWEGIVTRIWPNTKYL 302
           DISTGTL+ +I+DP+++E  +SK LT P+ ELA+FI+  C  +  WEGI+T+IWPNTKYL
Sbjct: 251 DISTGTLSSRISDPAIKES-MSKILTKPDQELADFITSVCGQDNSWEGIITKIWPNTKYL 310

Query: 303 DVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLKPMCKPSEVTYTIMPNMGYF 362
           DVIVTGAMAQYIP LE+YSGGLPMACTMYASSE YFG+NLKPMCKPSEV+YTIMPNM YF
Sbjct: 311 DVIVTGAMAQYIPMLEYYSGGLPMACTMYASSESYFGINLKPMCKPSEVSYTIMPNMAYF 370

Query: 363 EFLPHDTSSPPALSRDSPPRLVDLADVEVGKEYELVITTYSGLCRYRVGDILQVTGFHNA 422
           EFLPH    P   S      LV+LADVEVGKEYELVITTY+GL RYRVGDILQVTGF+N+
Sbjct: 371 EFLPHH-EVPTEKS-----ELVELADVEVGKEYELVITTYAGLNRYRVGDILQVTGFYNS 430

Query: 423 APQFRFVRRKNVLLSIDSDKTDEAELQKAIDKAALLLREFNTSVVEYTSYADTKTIPGHY 482
           APQF+FVRRKNVLLSI+SDKTDEAELQ A++ A+LLL E  T V+EYTSYA+TKTIPGHY
Sbjct: 431 APQFKFVRRKNVLLSIESDKTDEAELQSAVENASLLLGEQGTRVIEYTSYAETKTIPGHY 490

Query: 483 VIYWELHVKDAANPPPGEVLNQCCLAMEESLNSVYRQCRVADNSIGPLEIRVVKNGTFEE 542
           VIYWEL VKD  NPP  EV+ +CCL MEESLNSVYRQ RVAD SIGPLEIRVVKNGTFEE
Sbjct: 491 VIYWELLVKDQTNPPNDEVMARCCLEMEESLNSVYRQSRVADKSIGPLEIRVVKNGTFEE 550

Query: 543 LMDYAISRGASINQYKAPRCVNFTPIVELLDSRVTSAHFSPSKPQWTPERRR 594
           LMDYAISRGASINQYK PRCV+FTPI+ELLDSRV S HFSP+ P W+PERRR
Sbjct: 551 LMDYAISRGASINQYKVPRCVSFTPIMELLDSRVVSTHFSPALPHWSPERRR 595

[Close]

BLAST of Spg012164 vs. ExPASy Swiss-Prot
Match: A3BLS0 (Indole-3-acetic acid-amido synthetase GH3.8 OS=Oryza sativa subsp. indica OX=39946 GN=GH3.8 PE=1 SV=2)

HSP 1 Score: 880.2 bits (2273), Expect = 1.3e-254
Identity = 434/579 (74.96%), Postives = 493/579 (85.15%), Query Frame = 0


Query: 15  LHFIEETTTNADSVQQTVLADILNRNADTEYLNRFRLNGATDRDTFKSKVPVVTYEDLQP 74
           L FI+E TTN D+VQ+ VL +IL RNA TEYL +  L+GATDR  F++KVPVV+Y+DLQP
Sbjct: 30  LRFIDEMTTNVDAVQERVLGEILGRNAGTEYLTKCGLDGATDRAAFRAKVPVVSYDDLQP 89

Query: 75  DIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKEEMERRQLLYSLLMPIMNLY 134
            IQRIANGDRSPI S+HP+SEFLTSSGTSAGERKLMPTI +E++RRQLLYSLLMP+MNLY
Sbjct: 90  YIQRIANGDRSPILSTHPVSEFLTSSGTSAGERKLMPTIMDELDRRQLLYSLLMPVMNLY 149

Query: 135 VPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSDIFKTRPYDPFNDYTSPNEAILC 194
           VPGLDKGKGLYFLFVK+ETKTPGGL ARPVLTSYYKSD FK RPYDP+++YTSP  AILC
Sbjct: 150 VPGLDKGKGLYFLFVKSETKTPGGLTARPVLTSYYKSDHFKNRPYDPYHNYTSPTAAILC 209

Query: 195 PNSFQSMYTQMLCGLLMRGQVLRVGAVFASGLLRAIHFLQLNWKQFAHDISTGTLNPKIT 254
            ++FQSMY QM+CGL  R  VLR+GAVFASGLLRAI FLQLNW+Q A DI +G L P++T
Sbjct: 210 ADAFQSMYAQMVCGLCQRNDVLRLGAVFASGLLRAIRFLQLNWEQLADDIESGELTPRVT 269

Query: 255 DPSLRECIISKYLTNPNPELAEFISKECSTEEWEGIVTRIWPNTKYLDVIVTGAMAQYIP 314
           DPS+RE + +  L  P+PELA+ I  ECS  +W GI+TR+WPNTKYLDVIVTGAMAQYIP
Sbjct: 270 DPSVREAVAAILL--PDPELAKLIRAECSKGDWAGIITRVWPNTKYLDVIVTGAMAQYIP 329

Query: 315 TLEFYSGGLPMACTMYASSECYFGVNLKPMCKPSEVTYTIMPNMGYFEFLPHDTSSPPAL 374
           TLEFYSGGLPMACTMYASSECYFG+NL+PMC PSEV+YTIMPNMGYFEFLP D +   A 
Sbjct: 330 TLEFYSGGLPMACTMYASSECYFGLNLRPMCDPSEVSYTIMPNMGYFEFLPVDETG--AA 389

Query: 375 SRDSPPRLVDLADVEVGKEYELVITTYSGLCRYRVGDILQVTGFHNAAPQFRFVRRKNVL 434
           S D+  +LVDLA VEVG+EYELVITTY+GL RYRVGD+L+VTGFHNAAPQFRFVRRKNVL
Sbjct: 390 SGDA-TQLVDLARVEVGREYELVITTYAGLNRYRVGDVLRVTGFHNAAPQFRFVRRKNVL 449

Query: 435 LSIDSDKTDEAELQKAIDKAALLLREFNTSVVEYTSYADTKTIPGHYVIYWELHVKDA-A 494
           LSI+SDKTDEAELQ+A+++A+ LLR    SVVEYTS A TK IPGHYVIYWEL  K A A
Sbjct: 450 LSIESDKTDEAELQRAVERASALLRPHGASVVEYTSQACTKRIPGHYVIYWELLTKGAGA 509

Query: 495 NPPPGEVLNQCCLAMEESLNSVYRQCRVADNSIGPLEIRVVKNGTFEELMDYAISRGASI 554
                + L +CCL MEE+LN+VYRQ RVAD SIGPLEIRVV+ GTFEELMDYAISRGASI
Sbjct: 510 TVVDADTLGRCCLEMEEALNTVYRQSRVADGSIGPLEIRVVRPGTFEELMDYAISRGASI 569

Query: 555 NQYKAPRCVNFTPIVELLDSRVTSAHFSPSKPQWTPERR 593
           NQYK PRCV F PIVELLDSRV S+HFSP+ P WTP RR
Sbjct: 570 NQYKVPRCVTFPPIVELLDSRVVSSHFSPALPHWTPARR 603

[Close]

BLAST of Spg012164 vs. ExPASy Swiss-Prot
Match: Q0D4Z6 (Indole-3-acetic acid-amido synthetase GH3.8 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.8 PE=1 SV=1)

HSP 1 Score: 880.2 bits (2273), Expect = 1.3e-254
Identity = 434/579 (74.96%), Postives = 493/579 (85.15%), Query Frame = 0


Query: 15  LHFIEETTTNADSVQQTVLADILNRNADTEYLNRFRLNGATDRDTFKSKVPVVTYEDLQP 74
           L FI+E TTN D+VQ+ VL +IL RNA TEYL +  L+GATDR  F++KVPVV+Y+DLQP
Sbjct: 30  LRFIDEMTTNVDAVQERVLGEILGRNAGTEYLTKCGLDGATDRAAFRAKVPVVSYDDLQP 89

Query: 75  DIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKEEMERRQLLYSLLMPIMNLY 134
            IQRIANGDRSPI S+HP+SEFLTSSGTSAGERKLMPTI +E++RRQLLYSLLMP+MNLY
Sbjct: 90  YIQRIANGDRSPILSTHPVSEFLTSSGTSAGERKLMPTIMDELDRRQLLYSLLMPVMNLY 149

Query: 135 VPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSDIFKTRPYDPFNDYTSPNEAILC 194
           VPGLDKGKGLYFLFVK+ETKTPGGL ARPVLTSYYKSD FK RPYDP+++YTSP  AILC
Sbjct: 150 VPGLDKGKGLYFLFVKSETKTPGGLTARPVLTSYYKSDHFKNRPYDPYHNYTSPTAAILC 209

Query: 195 PNSFQSMYTQMLCGLLMRGQVLRVGAVFASGLLRAIHFLQLNWKQFAHDISTGTLNPKIT 254
            ++FQSMY QM+CGL  R  VLR+GAVFASGLLRAI FLQLNW+Q A DI +G L P++T
Sbjct: 210 ADAFQSMYAQMVCGLCQRNDVLRLGAVFASGLLRAIRFLQLNWEQLADDIESGELTPRVT 269

Query: 255 DPSLRECIISKYLTNPNPELAEFISKECSTEEWEGIVTRIWPNTKYLDVIVTGAMAQYIP 314
           DPS+RE + +  L  P+PELA+ I  ECS  +W GI+TR+WPNTKYLDVIVTGAMAQYIP
Sbjct: 270 DPSVREAVAAILL--PDPELAKLIRAECSKGDWAGIITRVWPNTKYLDVIVTGAMAQYIP 329

Query: 315 TLEFYSGGLPMACTMYASSECYFGVNLKPMCKPSEVTYTIMPNMGYFEFLPHDTSSPPAL 374
           TLEFYSGGLPMACTMYASSECYFG+NL+PMC PSEV+YTIMPNMGYFEFLP D +   A 
Sbjct: 330 TLEFYSGGLPMACTMYASSECYFGLNLRPMCDPSEVSYTIMPNMGYFEFLPVDETG--AA 389

Query: 375 SRDSPPRLVDLADVEVGKEYELVITTYSGLCRYRVGDILQVTGFHNAAPQFRFVRRKNVL 434
           S D+  +LVDLA VEVG+EYELVITTY+GL RYRVGD+L+VTGFHNAAPQFRFVRRKNVL
Sbjct: 390 SGDA-TQLVDLARVEVGREYELVITTYAGLNRYRVGDVLRVTGFHNAAPQFRFVRRKNVL 449

Query: 435 LSIDSDKTDEAELQKAIDKAALLLREFNTSVVEYTSYADTKTIPGHYVIYWELHVKDA-A 494
           LSI+SDKTDEAELQ+A+++A+ LLR    SVVEYTS A TK IPGHYVIYWEL  K A A
Sbjct: 450 LSIESDKTDEAELQRAVERASALLRPHGASVVEYTSQACTKRIPGHYVIYWELLTKGAGA 509

Query: 495 NPPPGEVLNQCCLAMEESLNSVYRQCRVADNSIGPLEIRVVKNGTFEELMDYAISRGASI 554
                + L +CCL MEE+LN+VYRQ RVAD SIGPLEIRVV+ GTFEELMDYAISRGASI
Sbjct: 510 TVVDADTLGRCCLEMEEALNTVYRQSRVADGSIGPLEIRVVRPGTFEELMDYAISRGASI 569

Query: 555 NQYKAPRCVNFTPIVELLDSRVTSAHFSPSKPQWTPERR 593
           NQYK PRCV F PIVELLDSRV S+HFSP+ P WTP RR
Sbjct: 570 NQYKVPRCVTFPPIVELLDSRVVSSHFSPALPHWTPARR 603

[Close]

BLAST of Spg012164 vs. ExPASy Swiss-Prot
Match: P0C0M2 (Probable indole-3-acetic acid-amido synthetase GH3.2 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.2 PE=2 SV=1)

HSP 1 Score: 875.9 bits (2262), Expect = 2.5e-253
Identity = 427/588 (72.62%), Postives = 503/588 (85.54%), Query Frame = 0


Query: 12  AKALHFIEETTTNADSVQQTVLADILNRNADTEYLNRFRLNGATDRDTFKSKVPVVTYED 71
           A+ L FIEE T   D+VQ+ VLA IL RN   EYL R  + G TDR+ FK++VPVVTYED
Sbjct: 30  AEKLEFIEEMTRGFDAVQERVLAAILARNNGAEYLRRHGMEGRTDREAFKARVPVVTYED 89

Query: 72  LQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKEEMERRQLLYSLLMPIM 131
           L+P+I+RIANGDRS I SSHPI+EFLTSSGTSAGERKLMPTI++E++RRQ+LYSLLMP+M
Sbjct: 90  LRPEIERIANGDRSNIISSHPITEFLTSSGTSAGERKLMPTIEDELDRRQMLYSLLMPVM 149

Query: 132 NLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSDIFKTRPYDPFNDYTSPNEA 191
           NLYVPGLDKGKGLYFLF+K+ETKTPGGL ARPVLTSYYKSD FK RP+DP+N YTSP  A
Sbjct: 150 NLYVPGLDKGKGLYFLFIKSETKTPGGLPARPVLTSYYKSDHFKHRPFDPYNVYTSPTAA 209

Query: 192 ILCPNSFQSMYTQMLCGLLMRGQVLRVGAVFASGLLRAIHFLQLNWKQFAHDISTGTLNP 251
           ILC ++FQSMY QMLCGL+ R +VLRVGAVFASGLLRAI FLQL+W++ AHDI TGTL+ 
Sbjct: 210 ILCTDAFQSMYAQMLCGLVARAEVLRVGAVFASGLLRAIRFLQLHWRELAHDIRTGTLSA 269

Query: 252 KITDPSLRECIISKYLTNPNPELAEFISKECSTEEWEGIVTRIWPNTKYLDVIVTGAMAQ 311
           K+T+PS+R+  +++ L  P+ ELA F+  EC  ++WEGI+TR+WPNTKYLDVIVTGAMAQ
Sbjct: 270 KVTEPSIRDA-VAEVLAAPDAELAAFVEAECGKDKWEGIITRMWPNTKYLDVIVTGAMAQ 329

Query: 312 YIPTLEFYSGGLPMACTMYASSECYFGVNLKPMCKPSEVTYTIMPNMGYFEFLPHDTSSP 371
           YIPTL+FYSGGLPMACTMYASSECYFG+NL+PMC PSEV+YTIMPNMGYFE +PHD  +P
Sbjct: 330 YIPTLKFYSGGLPMACTMYASSECYFGLNLRPMCDPSEVSYTIMPNMGYFELMPHDPDAP 389

Query: 372 PALSRDS-PPRLVDLADVEVGKEYELVITTYSGLCRYRVGDILQVTGFHNAAPQFRFVRR 431
           P L RD+ PPRLVDLAD EVG+EYELVITTY+GLCRYRVGDILQVTGFHNAAPQFRFVRR
Sbjct: 390 P-LPRDAPPPRLVDLADAEVGREYELVITTYAGLCRYRVGDILQVTGFHNAAPQFRFVRR 449

Query: 432 KNVLLSIDSDKTDEAELQKAIDKAALLLREFNTSVVEYTSYADTKTIPGHYVIYWELHVK 491
           KNVLLSIDSDKTDEAELQ A+++A+ LL  +  S+VEYTS AD  TIPGHYV+YWEL V+
Sbjct: 450 KNVLLSIDSDKTDEAELQAAVERASALLSPYGASIVEYTSQADATTIPGHYVVYWELMVR 509

Query: 492 D-AANPPPGE-----VLNQCCLAMEESLNSVYRQCRVADNSIGPLEIRVVKNGTFEELMD 551
           +  A PPP E     V  +CCL MEE+LN+VYRQ R  + +IGPLEIRVV+ GTFEE+MD
Sbjct: 510 EGGAWPPPAEEEGRGVFERCCLEMEEALNAVYRQGRNGE-AIGPLEIRVVRAGTFEEVMD 569

Query: 552 YAISRGASINQYKAPRCVNFTPIVELLDSRVTSAHFSPSKPQWTPERR 593
           YAISRGASINQYKAPRCV+F PI+ELL+SRV S HFSP+ P+++P ++
Sbjct: 570 YAISRGASINQYKAPRCVSFGPIIELLNSRVISKHFSPACPKYSPHKK 614

[Close]

BLAST of Spg012164 vs. ExPASy TrEMBL
Match: A0A6J1IRM5 (probable indole-3-acetic acid-amido synthetase GH3.1 OS=Cucurbita maxima OX=3661 GN=LOC111478730 PE=3 SV=1)

HSP 1 Score: 1097.0 bits (2836), Expect = 0.0e+00
Identity = 542/603 (89.88%), Postives = 566/603 (93.86%), Query Frame = 0


Query: 1   MAVVSNSSLP--------HAKALHFIEETTTNADSVQQTVLADILNRNADTEYLNRFRLN 60
           MAV S  S P         AKAL FIEE TTNAD+VQQ VLA+IL+RNA+TEY+NRFRLN
Sbjct: 1   MAVGSQLSSPLPPPPSDRDAKALRFIEEVTTNADAVQQRVLAEILSRNANTEYVNRFRLN 60

Query: 61  GATDRDTFKSKVPVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPT 120
           GATDRDT+KSK+PVVTYEDLQPDIQRIANGDRSPIFSS PISEFLTSSGTSAGERKLMPT
Sbjct: 61  GATDRDTYKSKIPVVTYEDLQPDIQRIANGDRSPIFSSTPISEFLTSSGTSAGERKLMPT 120

Query: 121 IKEEMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSD 180
           I+EEMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSD
Sbjct: 121 IQEEMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSD 180

Query: 181 IFKTRPYDPFNDYTSPNEAILCPNSFQSMYTQMLCGLLMRGQVLRVGAVFASGLLRAIHF 240
           IFKTRPYDPFNDYTSPNEA+LC +SFQSMYTQMLCGLLMR QVLRVGAVFASGLLRAI F
Sbjct: 181 IFKTRPYDPFNDYTSPNEAVLCADSFQSMYTQMLCGLLMREQVLRVGAVFASGLLRAIRF 240

Query: 241 LQLNWKQFAHDISTGTLNPKITDPSLRECIISKYLTNPNPELAEFISKECSTEEWEGIVT 300
           LQLNWKQ AHDISTGTLNPKITDP LRECI+SKYLTNPNPE+AEFISKECSTEEWEGI+T
Sbjct: 241 LQLNWKQLAHDISTGTLNPKITDPCLRECIVSKYLTNPNPEVAEFISKECSTEEWEGIIT 300

Query: 301 RIWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLKPMCKPSEVTY 360
           RIWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLKPMCKPSEVTY
Sbjct: 301 RIWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLKPMCKPSEVTY 360

Query: 361 TIMPNMGYFEFLPHDTSSPPALSRDSPPRLVDLADVEVGKEYELVITTYSGLCRYRVGDI 420
           TIMPNMGYFEFLPH TSSPP LSRDSPPRLVDLADVEVGKEYELVITTY+GL RYRVGD+
Sbjct: 361 TIMPNMGYFEFLPHHTSSPPVLSRDSPPRLVDLADVEVGKEYELVITTYAGLRRYRVGDV 420

Query: 421 LQVTGFHNAAPQFRFVRRKNVLLSIDSDKTDEAELQKAIDKAALLLREFNTSVVEYTSYA 480
           L+VTGFHNAAPQFRFVRRKNVLLSID+DKTDEAELQKA++ A+ LLRE NTSVVEYTSYA
Sbjct: 421 LEVTGFHNAAPQFRFVRRKNVLLSIDADKTDEAELQKAVENASSLLRELNTSVVEYTSYA 480

Query: 481 DTKTIPGHYVIYWELHVKDAA--NPPPGEVLNQCCLAMEESLNSVYRQCRVADNSIGPLE 540
           DTKTIPGHYVIYWEL VKD A  +PPP E+LNQCCLA+EESLNSVYRQ RVADNSIGPLE
Sbjct: 481 DTKTIPGHYVIYWELLVKDEAANSPPPAEILNQCCLAIEESLNSVYRQGRVADNSIGPLE 540

Query: 541 IRVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVTSAHFSPSKPQWTPE 594
           IRVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRV S HFSP+KP WTPE
Sbjct: 541 IRVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVVSVHFSPTKPHWTPE 600

[Close]

BLAST of Spg012164 vs. ExPASy TrEMBL
Match: A0A6J1GH54 (probable indole-3-acetic acid-amido synthetase GH3.1 OS=Cucurbita moschata OX=3662 GN=LOC111453877 PE=3 SV=1)

HSP 1 Score: 1096.3 bits (2834), Expect = 0.0e+00
Identity = 542/603 (89.88%), Postives = 565/603 (93.70%), Query Frame = 0


Query: 1   MAVVSNSSLP--------HAKALHFIEETTTNADSVQQTVLADILNRNADTEYLNRFRLN 60
           MAV S  S P         AKAL FIEE TTN D+VQQ VLA+IL+RNA+TEY+NRFRLN
Sbjct: 1   MAVDSQLSSPLPPPPSDKDAKALRFIEEVTTNTDAVQQRVLAEILSRNANTEYVNRFRLN 60

Query: 61  GATDRDTFKSKVPVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPT 120
           GATDRDT+KSK+PVVTYEDLQPDIQRIANGDRSPIFSS PISEFLTSSGTSAGERKLMPT
Sbjct: 61  GATDRDTYKSKIPVVTYEDLQPDIQRIANGDRSPIFSSTPISEFLTSSGTSAGERKLMPT 120

Query: 121 IKEEMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSD 180
           I+EEMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSD
Sbjct: 121 IQEEMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSD 180

Query: 181 IFKTRPYDPFNDYTSPNEAILCPNSFQSMYTQMLCGLLMRGQVLRVGAVFASGLLRAIHF 240
           IFKTRPYDPFNDYTSPNEA+LC +SFQSMYTQMLCGLLMR QVLRVGAVFASGLLRAI F
Sbjct: 181 IFKTRPYDPFNDYTSPNEAVLCADSFQSMYTQMLCGLLMREQVLRVGAVFASGLLRAIRF 240

Query: 241 LQLNWKQFAHDISTGTLNPKITDPSLRECIISKYLTNPNPELAEFISKECSTEEWEGIVT 300
           LQLNWKQ AHDISTGTLNPKITDP LRECI+SKYLTN NPE+AEFISKECSTEEWEGI+T
Sbjct: 241 LQLNWKQLAHDISTGTLNPKITDPCLRECIVSKYLTNTNPEVAEFISKECSTEEWEGIIT 300

Query: 301 RIWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLKPMCKPSEVTY 360
           RIWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLKPMCKPSEVTY
Sbjct: 301 RIWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLKPMCKPSEVTY 360

Query: 361 TIMPNMGYFEFLPHDTSSPPALSRDSPPRLVDLADVEVGKEYELVITTYSGLCRYRVGDI 420
           TIMPNMGYFEFLPHDTSSPP LSRDSPPRLVDLADVEVGKEYELVITTY+GL RYRVGD+
Sbjct: 361 TIMPNMGYFEFLPHDTSSPPVLSRDSPPRLVDLADVEVGKEYELVITTYAGLRRYRVGDV 420

Query: 421 LQVTGFHNAAPQFRFVRRKNVLLSIDSDKTDEAELQKAIDKAALLLREFNTSVVEYTSYA 480
           L+VTGFHNAAPQFRFVRRKNVLLSID+DKTDEAELQKA++ A+ LLRE NTSVVEYTSYA
Sbjct: 421 LEVTGFHNAAPQFRFVRRKNVLLSIDADKTDEAELQKAVENASSLLRELNTSVVEYTSYA 480

Query: 481 DTKTIPGHYVIYWELHVKDAA--NPPPGEVLNQCCLAMEESLNSVYRQCRVADNSIGPLE 540
           DTKTIPGHYVIYWEL VKD A  +PPP E+LNQCCLA+EESLNSVYRQ RVADNSIGPLE
Sbjct: 481 DTKTIPGHYVIYWELLVKDEAANSPPPAEILNQCCLAIEESLNSVYRQGRVADNSIGPLE 540

Query: 541 IRVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVTSAHFSPSKPQWTPE 594
           IRVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRV S HFSPSKP WTPE
Sbjct: 541 IRVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVASVHFSPSKPHWTPE 600

[Close]

BLAST of Spg012164 vs. ExPASy TrEMBL
Match: A0A6J1ILA9 (indole-3-acetic acid-amido synthetase GH3.3-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111478067 PE=3 SV=1)

HSP 1 Score: 1094.3 bits (2829), Expect = 0.0e+00
Identity = 540/586 (92.15%), Postives = 558/586 (95.22%), Query Frame = 0


Query: 6   NSSLPHAKALHFIEETTTNADSVQQTVLADILNRNADTEYLNRFRLNGATDRDTFKSKVP 65
           +SS+ HAKAL FIEETTTNADSVQ+ VLA+ILNRNADTEYLNRFRLNGATD+D FKSKVP
Sbjct: 4   DSSVSHAKALRFIEETTTNADSVQKRVLAEILNRNADTEYLNRFRLNGATDQDAFKSKVP 63

Query: 66  VVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKEEMERRQLLYS 125
           VVTYE+L+ DI+RIANGDRSPIFSSHPISEFLTSSGTS GERKLMPTIKEEMERRQLLYS
Sbjct: 64  VVTYEELEADIRRIANGDRSPIFSSHPISEFLTSSGTSGGERKLMPTIKEEMERRQLLYS 123

Query: 126 LLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSDIFKTRPYDPFNDY 185
           LLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSDIFKTRPYDPFNDY
Sbjct: 124 LLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSDIFKTRPYDPFNDY 183

Query: 186 TSPNEAILCPNSFQSMYTQMLCGLLMRGQVLRVGAVFASGLLRAIHFLQLNWKQFAHDIS 245
           TSPNEAILC NSFQSMYTQMLCGLLMR QVLRVGAVFASGLLRAIHFLQLNWKQ A+DIS
Sbjct: 184 TSPNEAILCANSFQSMYTQMLCGLLMRHQVLRVGAVFASGLLRAIHFLQLNWKQLAYDIS 243

Query: 246 TGTLNPKITDPSLRECIISKYLTNPNPELAEFISKECSTEEWEGIVTRIWPNTKYLDVIV 305
           TGTL+PKITDP LRECI SKYLTNPNPELAEFISKECSTEEWEGIVTRIWPNTKYLDVIV
Sbjct: 244 TGTLSPKITDPCLRECITSKYLTNPNPELAEFISKECSTEEWEGIVTRIWPNTKYLDVIV 303

Query: 306 TGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLKPMCKPSEVTYTIMPNMGYFEFLP 365
           TGAMAQYIPTLE YSGGLPMACTMYASSECYFG+NL PMCKPSEVTYTIMPNMGYFEFLP
Sbjct: 304 TGAMAQYIPTLELYSGGLPMACTMYASSECYFGLNLNPMCKPSEVTYTIMPNMGYFEFLP 363

Query: 366 HDTSSPPALSRDSPPRLVDLADVEVGKEYELVITTYSGLCRYRVGDILQVTGFHNAAPQF 425
           H TSSPPA SRDSPPRLV+LADVEVGK YELVITTYSGL RYRVGDILQVTGFHNAAPQF
Sbjct: 364 HHTSSPPAHSRDSPPRLVNLADVEVGKVYELVITTYSGLNRYRVGDILQVTGFHNAAPQF 423

Query: 426 RFVRRKNVLLSIDSDKTDEAELQKAIDKAALLLREFNTSVVEYTSYADTKTIPGHYVIYW 485
           RFVRRKNVLLSIDSDKTDEAELQ AI+ AALLLREFNTSV+EYT++ADTKTIPGHYVIY 
Sbjct: 424 RFVRRKNVLLSIDSDKTDEAELQTAINNAALLLREFNTSVLEYTTHADTKTIPGHYVIYL 483

Query: 486 ELHVKDAANPPPGEVLNQCCLAMEESLNSVYRQCRVADNSIGPLEIRVVKNGTFEELMDY 545
           EL VKD ANPPP E LNQCCLAMEESLNSVYRQCRVADNSIGPLEIRVVK  TFEE+MDY
Sbjct: 484 ELLVKDTANPPPVETLNQCCLAMEESLNSVYRQCRVADNSIGPLEIRVVKYRTFEEVMDY 543

Query: 546 AISRGASINQYKAPRCVNFTPIVELLDSRVTSAHFSPSKPQWTPER 592
           AISRGASINQYKAPRCVNFTPI+ELLDSRV SAHFSPSKP WTPER
Sbjct: 544 AISRGASINQYKAPRCVNFTPILELLDSRVISAHFSPSKPHWTPER 589

[Close]

BLAST of Spg012164 vs. ExPASy TrEMBL
Match: A0A6J1GF82 (probable indole-3-acetic acid-amido synthetase GH3.1 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111453636 PE=3 SV=1)

HSP 1 Score: 1090.1 bits (2818), Expect = 0.0e+00
Identity = 538/588 (91.50%), Postives = 559/588 (95.07%), Query Frame = 0


Query: 6   NSSLPHAKALHFIEETTTNADSVQQTVLADILNRNADTEYLNRFRLNGATDRDTFKSKVP 65
           +SS+ HAKAL FIEETTTNADSVQ+ VLA+ILNRNADTEYLNRFRLNGATD+D FKSKVP
Sbjct: 4   DSSVSHAKALRFIEETTTNADSVQKRVLAEILNRNADTEYLNRFRLNGATDQDAFKSKVP 63

Query: 66  VVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKEEMERRQLLYS 125
           VVTYE+L+ DI+RIANGDRSPIFSSHPISEFLTSSGTS GERKLMPTIKEEMERRQLLYS
Sbjct: 64  VVTYEELEADIRRIANGDRSPIFSSHPISEFLTSSGTSGGERKLMPTIKEEMERRQLLYS 123

Query: 126 LLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSDIFKTRPYDPFNDY 185
           LLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSDIFKTRPYDPFNDY
Sbjct: 124 LLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSDIFKTRPYDPFNDY 183

Query: 186 TSPNEAILCPNSFQSMYTQMLCGLLMRGQVLRVGAVFASGLLRAIHFLQLNWKQFAHDIS 245
           TSPNEAILC NSFQSMYTQMLCGLLMR +VLRVGAVFASGLLRAIHFLQLNWKQ A+DIS
Sbjct: 184 TSPNEAILCANSFQSMYTQMLCGLLMRHRVLRVGAVFASGLLRAIHFLQLNWKQLAYDIS 243

Query: 246 TGTLNPKITDPSLRECIISKYLTNPNPELAEFISKECSTEEWEGIVTRIWPNTKYLDVIV 305
           TGTL+PKITDP LRECI SKYLTNPNPELAEFISKECSTEEWEGIVTRIWPNTKYLDVIV
Sbjct: 244 TGTLSPKITDPCLRECITSKYLTNPNPELAEFISKECSTEEWEGIVTRIWPNTKYLDVIV 303

Query: 306 TGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLKPMCKPSEVTYTIMPNMGYFEFLP 365
           TGAMAQYIPTLE YSGGLPMACTMYASSECYFG+NL PMCKPSEVTYTIMPNMGYFEFLP
Sbjct: 304 TGAMAQYIPTLELYSGGLPMACTMYASSECYFGLNLNPMCKPSEVTYTIMPNMGYFEFLP 363

Query: 366 HDTSSPPALSRDSPPRLVDLADVEVGKEYELVITTYSGLCRYRVGDILQVTGFHNAAPQF 425
           HDT SPPA S +SPPRLV+LADVEVGK YELVITTYSGL RYRVGDILQVTGFHNAAPQF
Sbjct: 364 HDTLSPPAHSHNSPPRLVNLADVEVGKVYELVITTYSGLNRYRVGDILQVTGFHNAAPQF 423

Query: 426 RFVRRKNVLLSIDSDKTDEAELQKAIDKAALLLREFNTSVVEYTSYADTKTIPGHYVIYW 485
           RFVRRKNVLLSIDSDKTDEAELQ AI+ AALLLREFNTSV+EYT++ADTKTIPGHYVIY 
Sbjct: 424 RFVRRKNVLLSIDSDKTDEAELQTAINNAALLLREFNTSVLEYTTHADTKTIPGHYVIYL 483

Query: 486 ELHVKDAANPPPGEVLNQCCLAMEESLNSVYRQCRVADNSIGPLEIRVVKNGTFEELMDY 545
           EL VKD AN PP E LNQCC+AMEESLNSVYRQCRVADNSIGPLEIRVVKNGTFEE+MDY
Sbjct: 484 ELLVKDTANRPPVETLNQCCVAMEESLNSVYRQCRVADNSIGPLEIRVVKNGTFEEVMDY 543

Query: 546 AISRGASINQYKAPRCVNFTPIVELLDSRVTSAHFSPSKPQWTPERRR 594
           AISRGASINQYKAPRCVNFTPI+ELLDSRV SAHFS SKPQWTPER R
Sbjct: 544 AISRGASINQYKAPRCVNFTPILELLDSRVISAHFSLSKPQWTPERTR 591

[Close]

BLAST of Spg012164 vs. ExPASy TrEMBL
Match: A0A5D3CNV8 (Putative indole-3-acetic acid-amido synthetase GH3.1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G004400 PE=3 SV=1)

HSP 1 Score: 1080.9 bits (2794), Expect = 0.0e+00
Identity = 539/607 (88.80%), Postives = 559/607 (92.09%), Query Frame = 0


Query: 1   MAVVSNSSL----------PHAKALHFIEETTTNADSVQQTVLADILNRNADTEYLNRFR 60
           MAVV NS              AKAL FIEETTTNAD VQQ VLA+IL +NA TEY+NRFR
Sbjct: 1   MAVVDNSEFSSPLGPPACEKDAKALRFIEETTTNADLVQQRVLAEILTQNAHTEYVNRFR 60

Query: 61  LNGATDRDTFKSKVPVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLM 120
           LNGATDRDTFKSK PVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLM
Sbjct: 61  LNGATDRDTFKSKFPVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLM 120

Query: 121 PTIKEEMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYK 180
           PTIKEEMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYK
Sbjct: 121 PTIKEEMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYK 180

Query: 181 SDIFKTRPYDPFNDYTSPNEAILCPNSFQSMYTQMLCGLLMRGQVLRVGAVFASGLLRAI 240
           SDIFKTRPYDPFNDYTSPNEA+LC +SFQSMYTQMLCGLLMR QVLRVGAVFASGLLRAI
Sbjct: 181 SDIFKTRPYDPFNDYTSPNEAVLCADSFQSMYTQMLCGLLMRDQVLRVGAVFASGLLRAI 240

Query: 241 HFLQLNWKQFAHDISTGTLNPKITDPSLRECIISKYLTNPNPELAEFISKECSTEEWEGI 300
            FLQLNWKQ AHDISTGTLNPKITD  LRECI SKYLTNPNPELAEFIS EC TEEWEGI
Sbjct: 241 RFLQLNWKQLAHDISTGTLNPKITDTCLRECITSKYLTNPNPELAEFISNECYTEEWEGI 300

Query: 301 VTRIWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLKPMCKPSEV 360
           +TRIWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNL PMCKPS+V
Sbjct: 301 ITRIWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDV 360

Query: 361 TYTIMPNMGYFEFLPHDTSS-PPALSRDSPPRLVDLADVEVGKEYELVITTYSGLCRYRV 420
           TYTIMPNMGYFEFLPHD+SS   ALSRDSPPRLVDLADVEVGKEYELVITTY+GLCRYRV
Sbjct: 361 TYTIMPNMGYFEFLPHDSSSRASALSRDSPPRLVDLADVEVGKEYELVITTYAGLCRYRV 420

Query: 421 GDILQVTGFHNAAPQFRFVRRKNVLLSIDSDKTDEAELQKAIDKAALLLREFNTSVVEYT 480
           GD+L+VTGFHNAAPQFRFVRRKNVLLSIDSDKTDEAELQKA++ A+ LLR+FNT+VVEYT
Sbjct: 421 GDVLEVTGFHNAAPQFRFVRRKNVLLSIDSDKTDEAELQKAVENASSLLRDFNTTVVEYT 480

Query: 481 SYADTKTIPGHYVIYWELHVKD---AANPPPGEVLNQCCLAMEESLNSVYRQCRVADNSI 540
           SYADTKTIPGHYVIYWEL VKD     + P  E+LNQCCLA+EESLNSVYRQ RVADNSI
Sbjct: 481 SYADTKTIPGHYVIYWELLVKDNETMNSHPTDEILNQCCLAIEESLNSVYRQGRVADNSI 540

Query: 541 GPLEIRVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVTSAHFSPSKPQ 594
           GPLEIRVV+NGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVTS HFSPSKP 
Sbjct: 541 GPLEIRVVRNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVTSVHFSPSKPH 600

[Close]

BLAST of Spg012164 vs. TAIR 10
Match: AT2G14960.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 928.7 bits (2399), Expect = 2.3e-270
Identity = 458/602 (76.08%), Postives = 514/602 (85.38%), Query Frame = 0


Query: 1   MAVVSNSSLP--------HAKALHFIEETTTNADSVQQTVLADILNRNADTEYLNRFRLN 60
           MAV SN S P         AKAL FIEE T NAD+VQ+ +LA+IL RNADTEYL RF L 
Sbjct: 1   MAVDSNLSSPLGPPACEKDAKALRFIEEMTRNADTVQENLLAEILARNADTEYLRRFNLC 60

Query: 61  GATDRDTFKSKVPVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPT 120
           GATDRDTFK+K+PV+TYEDLQP+IQRIA+GDRSPI S+HPISEFLTSSGTSAGERKLMPT
Sbjct: 61  GATDRDTFKTKIPVITYEDLQPEIQRIADGDRSPILSAHPISEFLTSSGTSAGERKLMPT 120

Query: 121 IKEEMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSD 180
           IKEE++RRQLLYSLLMP+MNLYVPGLDKGKG+YFLFVK+ETKTPGGL ARPVLTSYYKS+
Sbjct: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGMYFLFVKSETKTPGGLPARPVLTSYYKSE 180

Query: 181 IFKTRPYDPFNDYTSPNEAILCPNSFQSMYTQMLCGLLMRGQVLRVGAVFASGLLRAIHF 240
            F++RPYDP+N YTSPNEAILCP+SFQSMYTQMLCGLL R  VLRVGAVFASGLLRAI F
Sbjct: 181 HFRSRPYDPYNVYTSPNEAILCPDSFQSMYTQMLCGLLDRLSVLRVGAVFASGLLRAIRF 240

Query: 241 LQLNWKQFAHDISTGTLNPKITDPSLRECIISKYLTNPNPELAEFISKECSTEEWEGIVT 300
           LQL+W +FAHDI  G L+ +ITDPS+R+C+    +  P+P LAEFI +EC ++ WE I+T
Sbjct: 241 LQLHWSRFAHDIELGCLDSEITDPSIRQCMSG--ILKPDPVLAEFIRRECKSDNWEKIIT 300

Query: 301 RIWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLKPMCKPSEVTY 360
           RIWPNTKYLDVIVTGAMAQYIPTLE+YSGGLPMACTMYASSECYFG+NL PM KPSEV+Y
Sbjct: 301 RIWPNTKYLDVIVTGAMAQYIPTLEYYSGGLPMACTMYASSECYFGLNLNPMSKPSEVSY 360

Query: 361 TIMPNMGYFEFLPHDTSSPPALSRDSPPRLVDLADVEVGKEYELVITTYSGLCRYRVGDI 420
           TIMPNM YFEF+P   +           + V+L DV +GKEYELV+TTY+GLCRYRVGDI
Sbjct: 361 TIMPNMAYFEFIPLGGT-----------KAVELVDVNIGKEYELVVTTYAGLCRYRVGDI 420

Query: 421 LQVTGFHNAAPQFRFVRRKNVLLSIDSDKTDEAELQKAIDKAALLL-REFNTSVVEYTSY 480
           L+VTGFHN+APQF FVRRKNVLLSIDSDKTDE+ELQKA++ A+ +L  E  + V EYTSY
Sbjct: 421 LRVTGFHNSAPQFHFVRRKNVLLSIDSDKTDESELQKAVENASSILHEECGSRVAEYTSY 480

Query: 481 ADTKTIPGHYVIYWELHVKDAANPPPGEVLNQCCLAMEESLNSVYRQCRVADNSIGPLEI 540
           ADT TIPGHYV+YWEL V+D A  P  E L +CCL MEESLNSVYRQ RVADNS+GPLEI
Sbjct: 481 ADTSTIPGHYVLYWELLVRDGARQPSHETLTRCCLGMEESLNSVYRQSRVADNSVGPLEI 540

Query: 541 RVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVTSAHFSPSKPQWTPER 594
           RVV+NGTFEELMDYAISRGASINQYK PRCVNFTPIVELLDSRV SAHFSPS P WTPER
Sbjct: 541 RVVRNGTFEELMDYAISRGASINQYKVPRCVNFTPIVELLDSRVVSAHFSPSLPHWTPER 589

[Close]

BLAST of Spg012164 vs. TAIR 10
Match: AT2G23170.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 905.2 bits (2338), Expect = 2.8e-263
Identity = 451/592 (76.18%), Postives = 508/592 (85.81%), Query Frame = 0


Query: 3   VVSNSSLPHAKALHFIEETTTNADSVQQTVLADILNRNADTEYLNRFRLNGATDRDTFKS 62
           ++ + S    KAL FIEE T N D VQ+ V+ +IL+RN+DTEYL RF L G TDR TFK+
Sbjct: 11  MMHSPSTKDVKALRFIEEMTRNVDFVQKKVIREILSRNSDTEYLKRFGLKGFTDRKTFKT 70

Query: 63  KVPVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKEEMERRQL 122
           KVPVV Y+DL+P+IQRIANGDRS I SS+PI+EFLTSSGTSAGERKLMPTI E+M+RRQL
Sbjct: 71  KVPVVIYDDLKPEIQRIANGDRSMILSSYPITEFLTSSGTSAGERKLMPTIDEDMDRRQL 130

Query: 123 LYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSDIFKTRPYDPF 182
           LYSLLMP+MNLYVPGLDKGK LYFLFVK E+KTPGGL ARPVLTSYYKS+ FK RP DP+
Sbjct: 131 LYSLLMPVMNLYVPGLDKGKALYFLFVKTESKTPGGLPARPVLTSYYKSEQFKRRPNDPY 190

Query: 183 NDYTSPNEAILCPNSFQSMYTQMLCGLLMRGQVLRVGAVFASGLLRAIHFLQLNWKQFAH 242
           N YTSPNEAILCP+S QSMYTQMLCGLLMR +VLR+GAVFASGLLRAI FLQ NWK+ A 
Sbjct: 191 NVYTSPNEAILCPDSSQSMYTQMLCGLLMRHEVLRLGAVFASGLLRAIGFLQTNWKELAD 250

Query: 243 DISTGTLNPKITDPSLRECIISKYLTNPNPELAEFISKECSTE-EWEGIVTRIWPNTKYL 302
           DISTGTL+ +I+DP+++E  +SK LT P+ ELA+FI+  C  +  WEGI+T+IWPNTKYL
Sbjct: 251 DISTGTLSSRISDPAIKES-MSKILTKPDQELADFITSVCGQDNSWEGIITKIWPNTKYL 310

Query: 303 DVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLKPMCKPSEVTYTIMPNMGYF 362
           DVIVTGAMAQYIP LE+YSGGLPMACTMYASSE YFG+NLKPMCKPSEV+YTIMPNM YF
Sbjct: 311 DVIVTGAMAQYIPMLEYYSGGLPMACTMYASSESYFGINLKPMCKPSEVSYTIMPNMAYF 370

Query: 363 EFLPHDTSSPPALSRDSPPRLVDLADVEVGKEYELVITTYSGLCRYRVGDILQVTGFHNA 422
           EFLPH    P   S      LV+LADVEVGKEYELVITTY+GL RYRVGDILQVTGF+N+
Sbjct: 371 EFLPHH-EVPTEKS-----ELVELADVEVGKEYELVITTYAGLNRYRVGDILQVTGFYNS 430

Query: 423 APQFRFVRRKNVLLSIDSDKTDEAELQKAIDKAALLLREFNTSVVEYTSYADTKTIPGHY 482
           APQF+FVRRKNVLLSI+SDKTDEAELQ A++ A+LLL E  T V+EYTSYA+TKTIPGHY
Sbjct: 431 APQFKFVRRKNVLLSIESDKTDEAELQSAVENASLLLGEQGTRVIEYTSYAETKTIPGHY 490

Query: 483 VIYWELHVKDAANPPPGEVLNQCCLAMEESLNSVYRQCRVADNSIGPLEIRVVKNGTFEE 542
           VIYWEL VKD  NPP  EV+ +CCL MEESLNSVYRQ RVAD SIGPLEIRVVKNGTFEE
Sbjct: 491 VIYWELLVKDQTNPPNDEVMARCCLEMEESLNSVYRQSRVADKSIGPLEIRVVKNGTFEE 550

Query: 543 LMDYAISRGASINQYKAPRCVNFTPIVELLDSRVTSAHFSPSKPQWTPERRR 594
           LMDYAISRGASINQYK PRCV+FTPI+ELLDSRV S HFSP+ P W+PERRR
Sbjct: 551 LMDYAISRGASINQYKVPRCVSFTPIMELLDSRVVSTHFSPALPHWSPERRR 595

[Close]

BLAST of Spg012164 vs. TAIR 10
Match: AT4G37390.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 901.4 bits (2328), Expect = 4.0e-262
Identity = 448/593 (75.55%), Postives = 507/593 (85.50%), Query Frame = 0


Query: 3   VVSNSSLPHAKALHFIEETTTNADSVQQTVLADILNRNADTEYLNRFRLNGATDRDTFKS 62
           V + +S    KAL FIEE T N DSVQ+ VL +IL RN++TEYL RF L+G  DR TFKS
Sbjct: 12  VSATTSEKDVKALKFIEEMTRNPDSVQEKVLGEILTRNSNTEYLKRFDLDGVVDRKTFKS 71

Query: 63  KVPVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKEEMERRQL 122
           KVPVVTYEDL+P+IQRI+NGD SPI SSHPI+EFLTSSGTSAGERKLMPTI+E+++RRQL
Sbjct: 72  KVPVVTYEDLKPEIQRISNGDCSPILSSHPITEFLTSSGTSAGERKLMPTIEEDLDRRQL 131

Query: 123 LYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSDIFKTRPYDPF 182
           LYSLLMP+MNLYVPGLDKGKGLYFLFVK+E+KT GGL ARPVLTSYYKSD FK RPYDP+
Sbjct: 132 LYSLLMPVMNLYVPGLDKGKGLYFLFVKSESKTSGGLPARPVLTSYYKSDHFKRRPYDPY 191

Query: 183 NDYTSPNEAILCPNSFQSMYTQMLCGLLMRGQVLRVGAVFASGLLRAIHFLQLNWKQFAH 242
           N YTSPNEAILC +S QSMY QMLCGLLMR +VLR+GAVFASGLLRAI FLQ NWK+ A 
Sbjct: 192 NVYTSPNEAILCSDSSQSMYAQMLCGLLMRHEVLRLGAVFASGLLRAISFLQNNWKELAR 251

Query: 243 DISTGTLNPKITDPSLRECIISKYLTNPNPELAEFISKECSTEEWEGIVTRIWPNTKYLD 302
           DISTGTL+ +I DP+++   +SK LT P+ ELAEF+   CS E WEGI+T+IWPNTKYLD
Sbjct: 252 DISTGTLSSRIFDPAIKN-RMSKILTKPDQELAEFLVGVCSQENWEGIITKIWPNTKYLD 311

Query: 303 VIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLKPMCKPSEVTYTIMPNMGYFE 362
           VIVTGAMAQYIPTLE+YSGGLPMACTMYASSE YFG+NLKPMCKPSEV+YTIMPNM YFE
Sbjct: 312 VIVTGAMAQYIPTLEYYSGGLPMACTMYASSESYFGINLKPMCKPSEVSYTIMPNMAYFE 371

Query: 363 FLPHDTSSPPALSRD-SPPRLVDLADVEVGKEYELVITTYSGLCRYRVGDILQVTGFHNA 422
           FLPH+     A         LV+LA+VEVGKEYELVITTY+GL RYRVGDIL+VTGFHN+
Sbjct: 372 FLPHNHDGDGAAEASLDETSLVELANVEVGKEYELVITTYAGLYRYRVGDILRVTGFHNS 431

Query: 423 APQFRFVRRKNVLLSIDSDKTDEAELQKAIDKAALLLREFNTSVVEYTSYADTKTIPGHY 482
           APQF+F+RRKNVLLS++SDKTDEAELQKA++ A+ L  E  T V+EYTSYA+TKTIPGHY
Sbjct: 432 APQFKFIRRKNVLLSVESDKTDEAELQKAVENASRLFAEQGTRVIEYTSYAETKTIPGHY 491

Query: 483 VIYWELHVKDAANP-PPGEVLNQCCLAMEESLNSVYRQCRVADNSIGPLEIRVVKNGTFE 542
           VIYWEL  +D +N     EV+ +CCL MEESLNSVYRQ RVAD SIGPLEIRVV+NGTFE
Sbjct: 492 VIYWELLGRDQSNALMSEEVMAKCCLEMEESLNSVYRQSRVADKSIGPLEIRVVRNGTFE 551

Query: 543 ELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVTSAHFSPSKPQWTPERRR 594
           ELMDYAISRGASINQYK PRCV+FTPI+ELLDSRV SAHFSPS P W+PERRR
Sbjct: 552 ELMDYAISRGASINQYKVPRCVSFTPIMELLDSRVVSAHFSPSLPHWSPERRR 603

[Close]

BLAST of Spg012164 vs. TAIR 10
Match: AT1G59500.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 842.4 bits (2175), Expect = 2.2e-244
Identity = 422/587 (71.89%), Postives = 489/587 (83.30%), Query Frame = 0


Query: 7   SSLPHAKALHFIEETTTNADSVQQTVLADILNRNADTEYLNRFRLNGATDRDTFKSKVPV 66
           +S    KAL FIEE T N DSVQ+ VL +IL+RN++TEYL RF LNGA DR +FKSKVPV
Sbjct: 16  TSETEVKALKFIEEITRNPDSVQEKVLGEILSRNSNTEYLKRFDLNGAVDRKSFKSKVPV 75

Query: 67  VTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKEEMERRQLLYSL 126
           V YEDL+ DIQRI+NGDRSPI SSHPI+EFLTSSGTSAGERKLMPTI+E++ RRQLL +L
Sbjct: 76  VIYEDLKTDIQRISNGDRSPILSSHPITEFLTSSGTSAGERKLMPTIEEDINRRQLLGNL 135

Query: 127 LMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSDIFKTRPYDPFNDYT 186
           LMP+MNLYVPGLDKGKGLYFLFVK+E+ T GGL ARP LTSYYKSD F+T   D  + YT
Sbjct: 136 LMPVMNLYVPGLDKGKGLYFLFVKSESTTSGGLPARPALTSYYKSDYFRTSDSD--SVYT 195

Query: 187 SPNEAILCPNSFQSMYTQMLCGLLMRGQVLRVGAVFASGLLRAIHFLQLNWKQFAHDIST 246
           SP EAILC +S QSMYTQMLCGLLMR +V R+GAVF SGLLRAI FLQ NWK+ + DIST
Sbjct: 196 SPKEAILCCDSSQSMYTQMLCGLLMRHEVNRLGAVFPSGLLRAISFLQNNWKELSQDIST 255

Query: 247 GTLNPKITDPSLRECIISKYLTNPNPELAEFISKECSTEEWEGIVTRIWPNTKYLDVIVT 306
           GTL+ KI D +++   +S  L  P+ ELAEF+   CS E WEGI+T+IWPNTKYLDVIVT
Sbjct: 256 GTLSSKIFDHAIK-TRMSNILNKPDQELAEFLIGVCSQENWEGIITKIWPNTKYLDVIVT 315

Query: 307 GAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLKPMCKPSEVTYTIMPNMGYFEFLPH 366
           GAMA+YIP LE+YSGGLPMA  +YASSE YFG+NL PMCKPSEV+YTI PNM YFEFLPH
Sbjct: 316 GAMAEYIPMLEYYSGGLPMASMIYASSESYFGINLNPMCKPSEVSYTIFPNMAYFEFLPH 375

Query: 367 DTSSPPALSRDSPPRLVDLADVEVGKEYELVITTYSGLCRYRVGDILQVTGFHNAAPQFR 426
           +      +   S   LV+LADVEVGKEYELVITTY+GL RYRVGDIL+VTGFHN+APQF+
Sbjct: 376 NHDGDGGVEATS---LVELADVEVGKEYELVITTYAGLYRYRVGDILRVTGFHNSAPQFK 435

Query: 427 FVRRKNVLLSIDSDKTDEAELQKAIDKAALLLREFNTSVVEYTSYADTKTIPGHYVIYWE 486
           F+RR+NVLLSI+SDKTDEA+LQKA++ A+ LL E  T V+EYTSYADTKTIPGHYVIYWE
Sbjct: 436 FIRRENVLLSIESDKTDEADLQKAVENASRLLAEQGTRVIEYTSYADTKTIPGHYVIYWE 495

Query: 487 LHVKDAANP-PPGEVLNQCCLAMEESLNSVYRQCRVADNSIGPLEIRVVKNGTFEELMDY 546
           L  +D +N  P  EV+ +CCL MEESLN+VYRQ RV+D SIGPLEIRVV+NGTFEELMD+
Sbjct: 496 LLSRDQSNALPSDEVMAKCCLEMEESLNAVYRQSRVSDKSIGPLEIRVVQNGTFEELMDF 555

Query: 547 AISRGASINQYKAPRCVNFTPIVELLDSRVTSAHFSPSKPQWTPERR 593
           +ISRG+SINQYK PRCV+ TPI++LLDSRV SAHFSPS P W+PERR
Sbjct: 556 SISRGSSINQYKVPRCVSLTPIMKLLDSRVVSAHFSPSLPHWSPERR 596

[Close]

BLAST of Spg012164 vs. TAIR 10
Match: AT4G27260.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 800.4 bits (2066), Expect = 9.6e-232
Identity = 380/587 (64.74%), Postives = 468/587 (79.73%), Query Frame = 0


Query: 15  LHFIEETTTNADSVQQTVLADILNRNADTEYLNRFRLNGATDRDTFKSKVPVVTYEDLQP 74
           L  IEE T+NAD VQ+ VL +IL RNAD EYL R  LNG TDR+TFK+ +PV+TYED++P
Sbjct: 25  LQLIEELTSNADQVQRQVLEEILTRNADVEYLRRHDLNGRTDRETFKNIMPVITYEDIEP 84

Query: 75  DIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKEEMERRQLLYSLLMPIMNLY 134
           +I RIANGD+SPI SS PISEFLTSSGTS GERKLMPTI+EE++RR LLYSLLMP+M+ +
Sbjct: 85  EINRIANGDKSPILSSKPISEFLTSSGTSGGERKLMPTIEEELDRRSLLYSLLMPVMSQF 144

Query: 135 VPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSDIFKTRPYDPFNDYTSPNEAILC 194
           VPGL+ GKG+YFLF+K+E+KTPGGL ARPVLTSYYKS  FK RPYDP+ +YTSPNE ILC
Sbjct: 145 VPGLENGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKERPYDPYTNYTSPNETILC 204

Query: 195 PNSFQSMYTQMLCGLLMRGQVLRVGAVFASGLLRAIHFLQLNWKQFAHDISTGTLNPKIT 254
            +S+QSMY+QMLCGL    +VLRVGAVFASG +RAI FL+ +W +   DI TGTL+  IT
Sbjct: 205 SDSYQSMYSQMLCGLCQHQEVLRVGAVFASGFIRAIKFLEKHWIELVRDIRTGTLSSLIT 264

Query: 255 DPSLRECIISKYLTNPNPELAEFISKECSTEEWEGIVTRIWPNTKYLDVIVTGAMAQYIP 314
           DPS+RE +    +  P+P+LA+F+  EC    W+GI+TR+WPNTKY+DVIVTG M+QYIP
Sbjct: 265 DPSVREAVAK--ILKPSPKLADFVEFECKKSSWQGIITRLWPNTKYVDVIVTGTMSQYIP 324

Query: 315 TLEFYSGGLPMACTMYASSECYFGVNLKPMCKPSEVTYTIMPNMGYFEFLP--------H 374
           TL++YS GLP+ CTMYASSECYFGVNL+P+CKPSEV+YT++P+M YFEFLP        +
Sbjct: 325 TLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPSMAYFEFLPVHRNNGVTN 384

Query: 375 DTSSPPALSRDSPPRLVDLADVEVGKEYELVITTYSGLCRYRVGDILQVTGFHNAAPQFR 434
             + P AL+      LVDL DV++G+EYELV+TTY+GLCRYRVGD+L+VTGF N APQF 
Sbjct: 385 SINLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAGLCRYRVGDLLRVTGFKNKAPQFS 444

Query: 435 FVRRKNVLLSIDSDKTDEAELQKAIDKAALLLREFNTSVVEYTSYADTKTIPGHYVIYWE 494
           F+ RKNV+LSIDSDKTDE ELQ A+  A   L  F+ S+ EYTSYADT +IPGHYV++WE
Sbjct: 445 FICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTSYADTSSIPGHYVLFWE 504

Query: 495 LHVKDAANPPPGEVLNQCCLAMEESLNSVYRQCRVADNSIGPLEIRVVKNGTFEELMDYA 554
           L + D   P P  V   CCLA+EES N+VYRQ RV+D SIGPLEI++V+ GTF++LMDYA
Sbjct: 505 LCL-DGNTPIPPSVFEDCCLAVEESFNTVYRQGRVSDKSIGPLEIKIVEPGTFDKLMDYA 564

Query: 555 ISRGASINQYKAPRCVNFTPIVELLDSRVTSAHFSPSKPQWTPERRR 594
           IS GASINQYK PRCV F PI+ELL+SRV  ++FSP  P+W P  ++
Sbjct: 565 ISLGASINQYKTPRCVKFAPIIELLNSRVVDSYFSPKCPKWVPGHKQ 608

The following BLAST results are available for this feature:

BLAST of Spg012164 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6604151.1	0.0e+00	90.05	putative indole-3-acetic acid-amido synthetase, partial [Cucurbita argyrosperma ...	[more]
XP_023543141.1	0.0e+00	90.05	probable indole-3-acetic acid-amido synthetase GH3.1 [Cucurbita pepo subsp. pepo...	[more]
KAG7034313.1	0.0e+00	91.78	putative indole-3-acetic acid-amido synthetase GH3.1, partial [Cucurbita argyros...	[more]
XP_022978925.1	0.0e+00	89.88	probable indole-3-acetic acid-amido synthetase GH3.1 [Cucurbita maxima]	[more]
XP_022950930.1	0.0e+00	89.88	probable indole-3-acetic acid-amido synthetase GH3.1 [Cucurbita moschata]	[more]

BLAST of Spg012164 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O82333	3.3e-269	76.08	Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Arabidopsis thaliana OX=...	[more]
O22190	3.9e-262	76.18	Indole-3-acetic acid-amido synthetase GH3.3 OS=Arabidopsis thaliana OX=3702 GN=G...	[more]
A3BLS0	1.3e-254	74.96	Indole-3-acetic acid-amido synthetase GH3.8 OS=Oryza sativa subsp. indica OX=399...	[more]
Q0D4Z6	1.3e-254	74.96	Indole-3-acetic acid-amido synthetase GH3.8 OS=Oryza sativa subsp. japonica OX=3...	[more]
P0C0M2	2.5e-253	72.62	Probable indole-3-acetic acid-amido synthetase GH3.2 OS=Oryza sativa subsp. japo...	[more]

BLAST of Spg012164 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1IRM5	0.0e+00	89.88	probable indole-3-acetic acid-amido synthetase GH3.1 OS=Cucurbita maxima OX=3661...	[more]
A0A6J1GH54	0.0e+00	89.88	probable indole-3-acetic acid-amido synthetase GH3.1 OS=Cucurbita moschata OX=36...	[more]
A0A6J1ILA9	0.0e+00	92.15	indole-3-acetic acid-amido synthetase GH3.3-like isoform X1 OS=Cucurbita maxima ...	[more]
A0A6J1GF82	0.0e+00	91.50	probable indole-3-acetic acid-amido synthetase GH3.1 isoform X2 OS=Cucurbita mos...	[more]
A0A5D3CNV8	0.0e+00	88.80	Putative indole-3-acetic acid-amido synthetase GH3.1 OS=Cucumis melo var. makuwa...	[more]

BLAST of Spg012164 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G14960.1	2.3e-270	76.08	Auxin-responsive GH3 family protein	[more]
AT2G23170.1	2.8e-263	76.18	Auxin-responsive GH3 family protein	[more]
AT4G37390.1	4.0e-262	75.55	Auxin-responsive GH3 family protein	[more]
AT1G59500.1	2.2e-244	71.89	Auxin-responsive GH3 family protein	[more]
AT4G27260.1	9.6e-232	64.74	Auxin-responsive GH3 family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004993	GH3 family	PFAM	PF03321	GH3	coord: 18..564 e-value: 1.5E-202 score: 674.2
IPR004993	GH3 family	PANTHER	PTHR31901	GH3 DOMAIN-CONTAINING PROTEIN	coord: 10..593
None	No IPR available	PANTHER	PTHR31901:SF7	INDOLE-3-ACETIC ACID-AMIDO SYNTHETASE GH3.2-RELATED	coord: 10..593

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg012164.1	Spg012164.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0016881	acid-amino acid ligase activity