Spg012030 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg012030
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionTwo-pore potassium channel 3
Locationscaffold1: 1903952 .. 1907079 (-)
RNA-Seq ExpressionSpg012030
SyntenySpg012030
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAAAAAAGTTGTTCTTACATCTGGCTTATATTTTATCAAGCTATTTTTGTAACGGTTCAATGAAAACAAAAGAAGAAGGAAGAACAATAAACGGGAACAAAGGGAAAAAGAAAGAAATTCGATTCCCAATCACACGTTGTCTTCACAGGTTGGGTTGGCCGATCCGTTGTCAATGATCAATGCTCACCCTCTTGCAAATGGCGCGTTAAATGGCTTTCTTCTTCATTTTCCTCTTTATCGGATCGTCTCCTTCATTAATCCTTCGTCTTCCTCTTTCTTTGTAGTACGTACGCTCTTACTGCGCTCTGTAATGTAATTATGCAACTGGGTTTCCCTCGTTTTTCACCTTTGAAGGATTTCAGTTTCTGGGTCCCAACGATAAACTTCTCTCTTCTTAATTAACTCCCATTTGAGCTCTCACTTTGTCCGTTTTGACTTGAAATTCATTCCTGAAAATCGTTTCTTCGGTTTTCATTTCGACCCTTTATTGTTTCCATTCTTCTGATTCTGCAAAACCATTGGACTCGCGGCTGCTGTTTGCATCTTGGCCTGAAGGGTCAACCATCGCCATATCTGAATGGAGAAAGAGCCTCTTCTTCCGTATCTGAGCCCAAGAGGGAAACCGCCTCCTTTACCTCCTCAATTATGTCCGCTCCCTGAAAATGATGAAATTACTCTGCCTATGACGCCCACGGAGTTCAAAGACCGCCTGATCTTTGGCCCTTCATCGTCCCCTCAAGATGGGTCTCCTGCGTTCGACGCTTTGACGCTATCTCTTTCGTCATCGAGACCCTCTGCTTCTTCTTCTCTTCAGGACCCATCAACCCCTTTGGACCCGAAATCCCAATCGCAGTCTCAACAGGGGTGGCCTGTGGATTCCAATTACTCATGGCACAAAAGCAACCTCCATCGCTCCAAAACTGCACCTGCCATGGCGGTCATCAACGATGTCAACCATTCGCAGGAGCCCAAGCCACAATTTGGTAAACAATCTATAATCCGCCAAGCTGTTGTTCTTCTAATTATTTACTTATCGTTGGGCGTGCTTATCTATTGGATTAATCGTGATAAGTTTTCGGCTACCGAGACTCATCCTGTGGTCGATGCATTGTACTTTTGTATTGTAACGATGTGCACGATTGGGTATGGAGATATTACGCCAAATAGTGCGGCGACGAAGCTATTCTCAGTATTTTTTGTGTTGGTGGGCTTTGGGTTCATTGATATATTGCTCTCTGGAATGGTCAGTTATGTGCTTGATTTGCAGGAGAGTTATTTGTTGAGGAATGTAAAAAGGGGGGTGCAGCAGGAGTCTAGGAAATCCTATATAATTGATGTGAAGAAGGGAAGAATGAGGATTAGAATGAAAGTGGCATTGGCCTTGGGCGTTGTGATACTTTGTATAGGAATTGGCATGGGTGTTATGCATTTTGTTGAGAACCTGGGGTGGTTGGATTCATTTTATCTTTCAGTCATGTCTGTGACAACGGTTGGATATGGTGATCAGGCATTCAAGTCGATGACTGGTCGTATTTTTGCTTCAATCTGGTTGCTTGTATCGACCCTTGCTGTTGCTCGGGCGTTTCTTTATTTAGCAGAGGCAAGAGTAGATAAGCGCCATAGGATGATGGCAAAATGGATACTTGGTCAGGATATGACTGTCTCTGAGTTTCTTGCAGCTGATATTGACAACAATGGCTTTGTGAGGTATGCAATAAGATTATTCATTCAAACTCATTTACAGAAGTAGCTTGGAAAATTGGATTGGATGTTACTGATTCTGATATCTGGAAGTCTTAATTGTATTGTTTCATGAACATGCTTGATTATTTTTGCATAATTTTTGTTATGAAATTATCTACTTTGGTGTGTTTGAAGAGCCATCGGTTTAAGAAATGATTGTCTTAAATGTGCATTCGTATTATCTACCTTATGACTATGAATCTGTTAATGATTCTGTGTGACTTTTCAACTTTTATAGCGTCGGATCGCCTTCTATTTGACGTAATTAATGATAGAGTTTGATATTACTTATCAACAAACCTAGGGAATAATTCAAGATCTTCATGTCCTATCTCTTTCCCAAGTACAACCAACTAGCATATACAAAACCATACTCCTCTATTCGTCTTTCTCCCTATTGGTATTGCGACCCAAAATCTAGTTGCATTTAAAGGTCCCTTCCCCAAACTTATTACATTCAACCCTATTACTTTCCTCACTGGCTTCCTCTTGTAGACGTTTCTAATAGGGGGTCTATCAATTAAGCATGCTATTAATAAGAATCTTTTAGATAGAAAGAAAGAAAATTGTTGGACTTTTTAATGAAAGGGAAGAGACTTGGAACTTTGCTCATTTTAAGACATCTACTTGGTGTATCCTTCTTACTCTGTTTCGTAATGATGATTCTTTTGTTATTGACTCCAAATCAACAACTGAAGTGTCTTCTTGTAAACTCTGTCGATCTCCTTCTGTACTTTCCATACCATCATCGAAAAGTTTAGTTTTGTTAGGCTGGAATGAGTTTTTGCTTCTGTTATATCATGGATGGAGTAAAAGCAAGAGAATGCAGTTTCAGCTTCAACTTTTGGGAAGTTTATGGAAAGTTCATTTAGGAAGATGAATTAAAGGATATGATATTTTCAATATTGAAATATGGAAAAAAGACTGATAATCAAACCTCATGTTCATATACAAAGGGTGCATTCAAAGACTATGGATTTCACAGAGGAAAGTGTTGGGATACCAGTAGGTAAAAGCCCTTAGTAATTAGTTGGTAAATTTGTTAGAATTGAATGGTTACACATAGATTTAGGGAGCTGGAAATAGGCAGATTTTGAGAGAATATAGATGTCATAAAGTGCTTAGAACTTCCTAAGTTTTTCTTTTAACATTAATAGCGCATTTCCGTTATTTCTGCTTCCGACCAGAAGAACTATACAATTATTGTCGTAAGAAATTACTCTACAAACAAAGATTAGAAGAGCCTAGGAGGCCGTCTAAGCCTCCTCGAACTACTCACAAGTAGAAATCCCCTCAGTCAAAAGTCACTTCATCAATGTTTGTAGCATAAACCACTTAACTGTTTGGGAACGTAGGTGCAGAAGTCTTACAGTTGGACGAAATC

mRNA sequence

ATGGAGAAAGAGCCTCTTCTTCCGTATCTGAGCCCAAGAGGGAAACCGCCTCCTTTACCTCCTCAATTATGTCCGCTCCCTGAAAATGATGAAATTACTCTGCCTATGACGCCCACGGAGTTCAAAGACCGCCTGATCTTTGGCCCTTCATCGTCCCCTCAAGATGGGTCTCCTGCGTTCGACGCTTTGACGCTATCTCTTTCGTCATCGAGACCCTCTGCTTCTTCTTCTCTTCAGGACCCATCAACCCCTTTGGACCCGAAATCCCAATCGCAGTCTCAACAGGGGTGGCCTGTGGATTCCAATTACTCATGGCACAAAAGCAACCTCCATCGCTCCAAAACTGCACCTGCCATGGCGGTCATCAACGATGTCAACCATTCGCAGGAGCCCAAGCCACAATTTGGTAAACAATCTATAATCCGCCAAGCTGTTGTTCTTCTAATTATTTACTTATCGTTGGGCGTGCTTATCTATTGGATTAATCGTGATAAGTTTTCGGCTACCGAGACTCATCCTGTGGTCGATGCATTGTACTTTTGTATTGTAACGATGTGCACGATTGGGTATGGAGATATTACGCCAAATAGTGCGGCGACGAAGCTATTCTCAGTATTTTTTGTGTTGGTGGGCTTTGGGTTCATTGATATATTGCTCTCTGGAATGGTCAGTTATGTGCTTGATTTGCAGGAGAGTTATTTGTTGAGGAATGTAAAAAGGGGGGTGCAGCAGGAGTCTAGGAAATCCTATATAATTGATGTGAAGAAGGGAAGAATGAGGATTAGAATGAAAGTGGCATTGGCCTTGGGCGTTGTGATACTTTGTATAGGAATTGGCATGGGTGTTATGCATTTTGTTGAGAACCTGGGGTGGTTGGATTCATTTTATCTTTCAGTCATGTCTGTGACAACGGTTGGATATGGTGATCAGGCATTCAAGTCGATGACTGGTCGTATTTTTGCTTCAATCTGGTTGCTTGTATCGACCCTTGCTGTTGCTCGGGCGTTTCTTTATTTAGCAGAGGCAAGAGTAGATAAGCGCCATAGGATGATGGCAAAATGGATACTTGGTCAGGATATGACTGTCTCTGAGTTTCTTGCAGCTGATATTGACAACAATGGCTTTGTGAGGGTGCATTCAAAGACTATGGATTTCACAGAGGAAAGTGTTGGGATACCAGTAGGTGCAGAAGTCTTACAGTTGGACGAAATC

Coding sequence (CDS)

ATGGAGAAAGAGCCTCTTCTTCCGTATCTGAGCCCAAGAGGGAAACCGCCTCCTTTACCTCCTCAATTATGTCCGCTCCCTGAAAATGATGAAATTACTCTGCCTATGACGCCCACGGAGTTCAAAGACCGCCTGATCTTTGGCCCTTCATCGTCCCCTCAAGATGGGTCTCCTGCGTTCGACGCTTTGACGCTATCTCTTTCGTCATCGAGACCCTCTGCTTCTTCTTCTCTTCAGGACCCATCAACCCCTTTGGACCCGAAATCCCAATCGCAGTCTCAACAGGGGTGGCCTGTGGATTCCAATTACTCATGGCACAAAAGCAACCTCCATCGCTCCAAAACTGCACCTGCCATGGCGGTCATCAACGATGTCAACCATTCGCAGGAGCCCAAGCCACAATTTGGTAAACAATCTATAATCCGCCAAGCTGTTGTTCTTCTAATTATTTACTTATCGTTGGGCGTGCTTATCTATTGGATTAATCGTGATAAGTTTTCGGCTACCGAGACTCATCCTGTGGTCGATGCATTGTACTTTTGTATTGTAACGATGTGCACGATTGGGTATGGAGATATTACGCCAAATAGTGCGGCGACGAAGCTATTCTCAGTATTTTTTGTGTTGGTGGGCTTTGGGTTCATTGATATATTGCTCTCTGGAATGGTCAGTTATGTGCTTGATTTGCAGGAGAGTTATTTGTTGAGGAATGTAAAAAGGGGGGTGCAGCAGGAGTCTAGGAAATCCTATATAATTGATGTGAAGAAGGGAAGAATGAGGATTAGAATGAAAGTGGCATTGGCCTTGGGCGTTGTGATACTTTGTATAGGAATTGGCATGGGTGTTATGCATTTTGTTGAGAACCTGGGGTGGTTGGATTCATTTTATCTTTCAGTCATGTCTGTGACAACGGTTGGATATGGTGATCAGGCATTCAAGTCGATGACTGGTCGTATTTTTGCTTCAATCTGGTTGCTTGTATCGACCCTTGCTGTTGCTCGGGCGTTTCTTTATTTAGCAGAGGCAAGAGTAGATAAGCGCCATAGGATGATGGCAAAATGGATACTTGGTCAGGATATGACTGTCTCTGAGTTTCTTGCAGCTGATATTGACAACAATGGCTTTGTGAGGGTGCATTCAAAGACTATGGATTTCACAGAGGAAAGTGTTGGGATACCAGTAGGTGCAGAAGTCTTACAGTTGGACGAAATC

Protein sequence

MEKEPLLPYLSPRGKPPPLPPQLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDGSPAFDALTLSLSSSRPSASSSLQDPSTPLDPKSQSQSQQGWPVDSNYSWHKSNLHRSKTAPAMAVINDVNHSQEPKPQFGKQSIIRQAVVLLIIYLSLGVLIYWINRDKFSATETHPVVDALYFCIVTMCTIGYGDITPNSAATKLFSVFFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVKRGVQQESRKSYIIDVKKGRMRIRMKVALALGVVILCIGIGMGVMHFVENLGWLDSFYLSVMSVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQDMTVSEFLAADIDNNGFVRVHSKTMDFTEESVGIPVGAEVLQLDEI
Homology
BLAST of Spg012030 vs. NCBI nr
Match: KAA0052565.1 (two-pore potassium channel 3 [Cucumis melo var. makuwa] >TYK13260.1 two-pore potassium channel 3 [Cucumis melo var. makuwa])

HSP 1 Score: 696.0 bits (1795), Expect = 1.9e-196
Identity = 361/402 (89.80%), Postives = 373/402 (92.79%), Query Frame = 0

Query: 1   MEKEPLLPYLSPRGKPPPLPPQLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDGSPAF 60
           MEKEPLLPYLSPRGKPPP PPQLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQD SP F
Sbjct: 1   MEKEPLLPYLSPRGKPPPPPPQLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDASPIF 60

Query: 61  DALTLSLSSSRPSASSSLQDPSTPLDPKSQSQSQQGWPVDSNYSWHKSNLHRSKTAPAMA 120
           DALTLSLSSSRPSASSSLQDPSTPLDP+SQSQSQQ W VDSNY+W KSNLHRSKTAPAMA
Sbjct: 61  DALTLSLSSSRPSASSSLQDPSTPLDPRSQSQSQQAWLVDSNYTWPKSNLHRSKTAPAMA 120

Query: 121 VINDVNHSQEPKPQFGKQSIIRQAVVLLIIYLSLGVLIYWINRDKFSATETHPVVDALYF 180
           VINDVNHSQEPKPQFGKQSIIRQAVVLLI+YLSLGVLIYW+NRD F+A ETHPVVDALYF
Sbjct: 121 VINDVNHSQEPKPQFGKQSIIRQAVVLLIVYLSLGVLIYWLNRDNFAAQETHPVVDALYF 180

Query: 181 CIVTMCTIGYGDITPNSAATKLFSVFFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVKR 240
           CIVTMCTIGYGDITPNS ATKLFSV FVLVGFGFIDILLSGMVSYVLDLQESYLLRNVKR
Sbjct: 181 CIVTMCTIGYGDITPNSTATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVKR 240

Query: 241 GVQQESRKSYIIDVKKGRMRIRMKVALALGVVILCIGIGMGVMHFVENLGWLDSFYLSVM 300
           GV++ES KSYIIDVKKGRMRIRMKVALALGVVILCIG+GMGVMHFVENLGWLDSFYLSVM
Sbjct: 241 GVKRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGMGVMHFVENLGWLDSFYLSVM 300

Query: 301 SVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQDM 360
           SVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQDM
Sbjct: 301 SVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQDM 360

Query: 361 TVSEFLAADIDNNGFVRVHSKTMDFTEESVGIPVGAEVLQLD 403
           T+SEFLAADIDNNGFVR        +   V   +G +V   D
Sbjct: 361 TISEFLAADIDNNGFVRYALTLFIHSFREVAWKIGFDVTDSD 402

BLAST of Spg012030 vs. NCBI nr
Match: XP_008439665.1 (PREDICTED: two-pore potassium channel 3 [Cucumis melo])

HSP 1 Score: 692.6 bits (1786), Expect = 2.1e-195
Identity = 356/376 (94.68%), Postives = 365/376 (97.07%), Query Frame = 0

Query: 1   MEKEPLLPYLSPRGKPPPLPPQLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDGSPAF 60
           MEKEPLLPYLSPRGKPPP PPQLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQD SP F
Sbjct: 1   MEKEPLLPYLSPRGKPPPPPPQLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDASPIF 60

Query: 61  DALTLSLSSSRPSASSSLQDPSTPLDPKSQSQSQQGWPVDSNYSWHKSNLHRSKTAPAMA 120
           DALTLSLSSSRPSASSSLQDPSTPLDP+SQSQSQQ W VDSNY+W KSNLHRSKTAPAMA
Sbjct: 61  DALTLSLSSSRPSASSSLQDPSTPLDPRSQSQSQQAWLVDSNYTWPKSNLHRSKTAPAMA 120

Query: 121 VINDVNHSQEPKPQFGKQSIIRQAVVLLIIYLSLGVLIYWINRDKFSATETHPVVDALYF 180
           VINDVNHSQEPKPQFGKQSIIRQAVVLLI+YLSLGVLIYW+NRD F+A ETHPVVDALYF
Sbjct: 121 VINDVNHSQEPKPQFGKQSIIRQAVVLLIVYLSLGVLIYWLNRDNFAAQETHPVVDALYF 180

Query: 181 CIVTMCTIGYGDITPNSAATKLFSVFFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVKR 240
           CIVTMCTIGYGDITPNS ATKLFSV FVLVGFGFIDILLSGMVSYVLDLQESYLLRNVKR
Sbjct: 181 CIVTMCTIGYGDITPNSTATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVKR 240

Query: 241 GVQQESRKSYIIDVKKGRMRIRMKVALALGVVILCIGIGMGVMHFVENLGWLDSFYLSVM 300
           GV++ES KSYIIDVKKGRMRIRMKVALALGVVILCIG+GMGVMHFVENLGWLDSFYLSVM
Sbjct: 241 GVKRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGMGVMHFVENLGWLDSFYLSVM 300

Query: 301 SVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQDM 360
           SVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQDM
Sbjct: 301 SVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQDM 360

Query: 361 TVSEFLAADIDNNGFV 377
           T+SEFLAADIDNNGFV
Sbjct: 361 TISEFLAADIDNNGFV 376

BLAST of Spg012030 vs. NCBI nr
Match: KAE8647742.1 (hypothetical protein Csa_004248 [Cucumis sativus])

HSP 1 Score: 692.6 bits (1786), Expect = 2.1e-195
Identity = 356/377 (94.43%), Postives = 366/377 (97.08%), Query Frame = 0

Query: 1   MEKEPLLPYLSPRGKPPPLPPQLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDGSPAF 60
           MEKEPLLPYLSPRGKP P+PPQLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQD SP F
Sbjct: 1   MEKEPLLPYLSPRGKPSPIPPQLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDASPIF 60

Query: 61  DALTLSLSSSRPSASSSLQDPSTPLDPKSQSQSQQGWPVDSNYSWHKSNLHRSKTAPAMA 120
           DALTLSLSSSRPSASSSLQDPSTPLDP+SQSQSQQ W VDSNY+W KSNLHRS+TAPAMA
Sbjct: 61  DALTLSLSSSRPSASSSLQDPSTPLDPRSQSQSQQAWLVDSNYTWPKSNLHRSRTAPAMA 120

Query: 121 VINDVNHSQEPKPQFGKQSIIRQAVVLLIIYLSLGVLIYWINRDKFSATETHPVVDALYF 180
           VINDVNHSQEPKPQFGKQSIIRQAVVLLI+YLSLGVLIYW+NRD FSA ETHPVVDALYF
Sbjct: 121 VINDVNHSQEPKPQFGKQSIIRQAVVLLIVYLSLGVLIYWLNRDNFSAQETHPVVDALYF 180

Query: 181 CIVTMCTIGYGDITPNSAATKLFSVFFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVKR 240
           CIVTMCTIGYGDITPNS +TKLFSV FVLVGFGFIDILLSGMVSYVLDLQESYLLRNVKR
Sbjct: 181 CIVTMCTIGYGDITPNSTSTKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVKR 240

Query: 241 GVQQESRKSYIIDVKKGRMRIRMKVALALGVVILCIGIGMGVMHFVENLGWLDSFYLSVM 300
           GV++ES KSYIIDVKKGRMRIRMKVALALGVVILCIG+GMGVMHFVENLGWLDSFYLSVM
Sbjct: 241 GVKRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGMGVMHFVENLGWLDSFYLSVM 300

Query: 301 SVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQDM 360
           SVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQDM
Sbjct: 301 SVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQDM 360

Query: 361 TVSEFLAADIDNNGFVR 378
           TVSEFLAADIDNNGFVR
Sbjct: 361 TVSEFLAADIDNNGFVR 377

BLAST of Spg012030 vs. NCBI nr
Match: XP_004134597.1 (two-pore potassium channel 3 [Cucumis sativus])

HSP 1 Score: 690.6 bits (1781), Expect = 7.8e-195
Identity = 355/376 (94.41%), Postives = 365/376 (97.07%), Query Frame = 0

Query: 1   MEKEPLLPYLSPRGKPPPLPPQLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDGSPAF 60
           MEKEPLLPYLSPRGKP P+PPQLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQD SP F
Sbjct: 1   MEKEPLLPYLSPRGKPSPIPPQLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDASPIF 60

Query: 61  DALTLSLSSSRPSASSSLQDPSTPLDPKSQSQSQQGWPVDSNYSWHKSNLHRSKTAPAMA 120
           DALTLSLSSSRPSASSSLQDPSTPLDP+SQSQSQQ W VDSNY+W KSNLHRS+TAPAMA
Sbjct: 61  DALTLSLSSSRPSASSSLQDPSTPLDPRSQSQSQQAWLVDSNYTWPKSNLHRSRTAPAMA 120

Query: 121 VINDVNHSQEPKPQFGKQSIIRQAVVLLIIYLSLGVLIYWINRDKFSATETHPVVDALYF 180
           VINDVNHSQEPKPQFGKQSIIRQAVVLLI+YLSLGVLIYW+NRD FSA ETHPVVDALYF
Sbjct: 121 VINDVNHSQEPKPQFGKQSIIRQAVVLLIVYLSLGVLIYWLNRDNFSAQETHPVVDALYF 180

Query: 181 CIVTMCTIGYGDITPNSAATKLFSVFFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVKR 240
           CIVTMCTIGYGDITPNS +TKLFSV FVLVGFGFIDILLSGMVSYVLDLQESYLLRNVKR
Sbjct: 181 CIVTMCTIGYGDITPNSTSTKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVKR 240

Query: 241 GVQQESRKSYIIDVKKGRMRIRMKVALALGVVILCIGIGMGVMHFVENLGWLDSFYLSVM 300
           GV++ES KSYIIDVKKGRMRIRMKVALALGVVILCIG+GMGVMHFVENLGWLDSFYLSVM
Sbjct: 241 GVKRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGMGVMHFVENLGWLDSFYLSVM 300

Query: 301 SVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQDM 360
           SVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQDM
Sbjct: 301 SVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQDM 360

Query: 361 TVSEFLAADIDNNGFV 377
           TVSEFLAADIDNNGFV
Sbjct: 361 TVSEFLAADIDNNGFV 376

BLAST of Spg012030 vs. NCBI nr
Match: XP_023544618.1 (two-pore potassium channel 3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 684.5 bits (1765), Expect = 5.6e-193
Identity = 359/377 (95.23%), Postives = 363/377 (96.29%), Query Frame = 0

Query: 1   MEKEPLLPYLSPRGKPPPLPPQ-LCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDGSPA 60
           MEKEPLLPY+SPRGKP P PPQ LCPLPENDEITLPMTPTEFKDRLIFGPSSSPQD SP 
Sbjct: 1   MEKEPLLPYMSPRGKPLPPPPQLLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDTSPI 60

Query: 61  FDALTLSLSSSRPSASSSLQDPSTPLDPKSQSQSQQGWPVDSNYSWHKSNLHRSKTAPAM 120
           FDALTLSLSSSRPSASSSLQDPSTPL PKSQS SQQ W VDSNYSWHKSNLHRSKTAPAM
Sbjct: 61  FDALTLSLSSSRPSASSSLQDPSTPLAPKSQSPSQQAWLVDSNYSWHKSNLHRSKTAPAM 120

Query: 121 AVINDVNHSQEPKPQFGKQSIIRQAVVLLIIYLSLGVLIYWINRDKFSATETHPVVDALY 180
           AVINDVNHSQEPKPQFGKQSIIRQAVVLLI+YLSLGVLIYWINRDKFSA ETHPVVDALY
Sbjct: 121 AVINDVNHSQEPKPQFGKQSIIRQAVVLLILYLSLGVLIYWINRDKFSAHETHPVVDALY 180

Query: 181 FCIVTMCTIGYGDITPNSAATKLFSVFFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVK 240
           FCIVTMCTIGYGDITPNSAATKLFSV FVLVGFGFIDILLSGMVSYVLDLQESYLLRNVK
Sbjct: 181 FCIVTMCTIGYGDITPNSAATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVK 240

Query: 241 RGVQQESRKSYIIDVKKGRMRIRMKVALALGVVILCIGIGMGVMHFVENLGWLDSFYLSV 300
           RGVQ+ES KSYIIDVKKGRMRIRMKVALALGVVILCIG+GMGVMHFVENLGWLDSFYLSV
Sbjct: 241 RGVQRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGLGMGVMHFVENLGWLDSFYLSV 300

Query: 301 MSVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQD 360
           MSVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQD
Sbjct: 301 MSVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQD 360

Query: 361 MTVSEFLAADIDNNGFV 377
           MTVSEFLAAD DNNG V
Sbjct: 361 MTVSEFLAADTDNNGCV 377

BLAST of Spg012030 vs. ExPASy Swiss-Prot
Match: Q9SVV6 (Two-pore potassium channel 3 OS=Arabidopsis thaliana OX=3702 GN=TPK3 PE=2 SV=1)

HSP 1 Score: 442.2 bits (1136), Expect = 6.3e-123
Identity = 255/414 (61.59%), Postives = 312/414 (75.36%), Query Frame = 0

Query: 4   EPLLPY-LSPRGKPPPLPPQLCPLPENDE--ITLPMTPTEFKDRLIFGP-SSSPQDGSPA 63
           +PLL Y +SPR K P  P  L PLPE++E  I +PMTP+EFK+RLIFGP S SP+D S  
Sbjct: 7   DPLLQYMISPRLKKP--PQLLFPLPEDNEVAIPMPMTPSEFKERLIFGPFSCSPRDSSHF 66

Query: 64  FDALTL-SLSSSRPSASSSLQDPST--PLDPKSQSQSQQGWPVDSNYSWHKSN-LHRSKT 123
            D++   S SSS  + ++   D ST  PL P    Q +  W  D   S  + + LHRSKT
Sbjct: 67  IDSMKQPSPSSSSTAVNNPFSDSSTLDPLLPPPPPQPEP-WLSDQTSSHCQGHALHRSKT 126

Query: 124 APAMAVINDVNHS-QEPKPQFGKQSIIRQAVVLLIIYLSLGVLIYWINRDKFSATETHPV 183
           APAMAVIND++H  ++  P    +S++RQA  LL++YLSLGVLIYW+NRD +   +THPV
Sbjct: 127 APAMAVINDLHHPIRQKDPTETSRSVVRQAFALLVVYLSLGVLIYWLNRDHYVVNQTHPV 186

Query: 184 VDALYFCIVTMCTIGYGDITPNSAATKLFSVFFVLVGFGFIDILLSGMVSYVLDLQESYL 243
           VD LYFCIVTMCTIGYGDITPNS  TKLFS+ FVLVGFGFIDILLSGMVSYVLDLQESY+
Sbjct: 187 VDGLYFCIVTMCTIGYGDITPNSVVTKLFSIMFVLVGFGFIDILLSGMVSYVLDLQESYM 246

Query: 244 LRNVKRGVQQESRKSYIIDVKKGRMRIRMKVALALGVVILCIGIGMGVMHFVENLGWLDS 303
           L + KR  + E R+SYIIDVKKGRMRIR+KVALALGVV+LCI +G+G+MHF+E +GWLDS
Sbjct: 247 LDSAKRRDEPEKRRSYIIDVKKGRMRIRLKVALALGVVVLCIAVGVGIMHFIEEIGWLDS 306

Query: 304 FYLSVMSVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKW 363
           FYLSVMSVTTVGYGD+AFK++ GR+FA+IWLLVSTLAVARAFLYLAEARVDKR+R  AK 
Sbjct: 307 FYLSVMSVTTVGYGDRAFKTLPGRLFAAIWLLVSTLAVARAFLYLAEARVDKRNRERAKK 366

Query: 364 ILGQDMTVSEFLAADIDNNG------FVRVHSKTMDFTEESVGIPVGAEVLQLD 403
           +L + M+VS+F AADIDNNG      +V    K M+   +   +P+  +  +LD
Sbjct: 367 VLCETMSVSQFFAADIDNNGCVSKAEYVIYKLKEMEKITDKDILPISKQFDKLD 417

BLAST of Spg012030 vs. ExPASy Swiss-Prot
Match: Q9FL25 (Two-pore potassium channel 2 OS=Arabidopsis thaliana OX=3702 GN=TPK2 PE=2 SV=1)

HSP 1 Score: 406.8 bits (1044), Expect = 2.9e-112
Identity = 239/415 (57.59%), Postives = 301/415 (72.53%), Query Frame = 0

Query: 4   EPLLPYL-SPRGKPPPLPPQLCPLPENDEITLPM--TPTEFKDRLIFGP-SSSPQDGSPA 63
           +PL  YL +PR  PP  PP L  LPEN+++T+PM  TP E K+RLIFG    S ++ S  
Sbjct: 12  DPLRQYLMNPRINPP--PPSLLTLPENNDVTIPMPITPLELKNRLIFGSFVRSRKESSLP 71

Query: 64  FDALTLSLSSSRPSASSSLQDPSTPLDPKSQSQSQQGWPVDSNYSWHKSNLHRSKTAPAM 123
            DAL+ + S+S  SA++S  D +  L P ++       PV    S    N HRSKTAPAM
Sbjct: 72  IDALSQNPSTS-SSATTSFSDSTDLLLPLTEPNK----PV--RKSKPTINFHRSKTAPAM 131

Query: 124 AVINDVNHSQEPK--PQFGKQSIIRQAVVLLIIYLSLGVLIYWINRDKFSATETHPVVDA 183
           A IN+++H  +PK   Q   ++I+ QAV LL++YLSLGVLIYW+NRD ++  +THPVVDA
Sbjct: 132 AAINNISHPNDPKTDQQSDSKTIVNQAVALLVVYLSLGVLIYWLNRDSYNVKQTHPVVDA 191

Query: 184 LYFCIVTMCTIGYGDITPNSAATKLFSVFFVLVGFGFIDILLSGMVSYVLDLQESYLL-- 243
           LYFCIVTMCTIGYGDITP+S  TKLFS+FFVLVGFGF+DILLSGMV+YVLDLQE+Y+L  
Sbjct: 192 LYFCIVTMCTIGYGDITPDSVVTKLFSIFFVLVGFGFMDILLSGMVTYVLDLQENYMLET 251

Query: 244 -RNVKRGVQQESR-KSYIIDVKKGRMRIRMKVALALGVVILCIGIGMGVMHFVENLGWLD 303
            RN    +    + +SYIIDVKKGRMRIR+KV LALGVV+LC+G G+ +MHFVE +GWLD
Sbjct: 252 ARNESLNLNDRDKVRSYIIDVKKGRMRIRLKVGLALGVVVLCLGFGVLIMHFVEKIGWLD 311

Query: 304 SFYLSVMSVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAK 363
           SFY SVMSVTTVGYGD+AF ++ GR+ A++WLLVSTLAVARA L+LAE+RVDKR+R  AK
Sbjct: 312 SFYFSVMSVTTVGYGDRAFNTLAGRLLAAMWLLVSTLAVARAILFLAESRVDKRNRERAK 371

Query: 364 WILGQDMTVSEFLAADIDNNG------FVRVHSKTMDFTEESVGIPVGAEVLQLD 403
            +LG+ M++S+FL ADID NG      FV    K MD   E    P+G +  +LD
Sbjct: 372 KVLGESMSISQFLDADIDCNGCVSKAEFVIYKLKKMDKITEKDINPIGFQFDKLD 417

BLAST of Spg012030 vs. ExPASy Swiss-Prot
Match: Q69TN4 (Two pore potassium channel c OS=Oryza sativa subsp. japonica OX=39947 GN=TPKC PE=3 SV=1)

HSP 1 Score: 344.7 bits (883), Expect = 1.4e-93
Identity = 210/403 (52.11%), Postives = 264/403 (65.51%), Query Frame = 0

Query: 1   MEKEPLLPYLSPRGKPPPLPPQLCPLPENDEITL---PMTP-----TEFKDRLIFG---- 60
           M+ EPLL  LSP       P  L PLPE+ E++    P++P     + +K+R+IFG    
Sbjct: 1   MDTEPLLSPLSPS------PHLLHPLPEHAEVSTFSPPLSPCPSPASSYKERIIFGAHPP 60

Query: 61  ---PSSSPQDGSPAFDALTLSLSSSRPSASSSLQDPSTPLDPKSQSQSQQGWPVDSNYSW 120
              P   P    P        +S       S    PS P D ++   +    P   ++  
Sbjct: 61  PPPPPPPPPPPPPRGRRYYRRVSGDDLDVPSCSSSPSPPSDEENPPPNP---PSLFDFIG 120

Query: 121 HKSNLHRSKTAPAMAVINDV--------NHSQEPKPQFGKQSIIRQAVVLLIIYLSLGVL 180
            ++NLHRS+TAPAMA +N            S+ P P   + +I+  A + L+ YL++GV 
Sbjct: 121 GRTNLHRSRTAPAMAPLNAAAIAAAAASGDSRNPPPPPRRPAIVLHAFLFLLAYLAMGVT 180

Query: 181 IYWINRDKF--SATETHPVVDALYFCIVTMCTIGYGDITPNSAATKLFSVFFVLVGFGFI 240
            Y      F  SA  THPV DALYFCIVT+CTIGYGDITP + A KLFS+ FVL+GFGF+
Sbjct: 181 FYAALPGNFTSSAGPTHPVADALYFCIVTLCTIGYGDITPATPAAKLFSISFVLIGFGFV 240

Query: 241 DILLSGMVSYVLDLQESYLLRNVK--RGVQQESRKSYIIDVKKGRMRIRMKVALALGVVI 300
           DILLSGMVSYVLDLQE  L+  +K  R V++  R +YI D+KKGRMR+RMKVALAL VV 
Sbjct: 241 DILLSGMVSYVLDLQEHLLITALKNPRSVRKH-RHNYIFDLKKGRMRVRMKVALALTVVA 300

Query: 301 LCIGIGMGVMHFVENLGWLDSFYLSVMSVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVA 360
           +C+G+G  V+  VENLGWLD+ YL+VMSVTTVGYGD AF+++ GR+FAS WLLVSTLAVA
Sbjct: 301 ICVGVGAAVLKRVENLGWLDAVYLAVMSVTTVGYGDHAFRTLAGRLFASAWLLVSTLAVA 360

Query: 361 RAFLYLAEARVDKRHRMMAKWILGQDMTVSEFLAADIDNNGFV 377
           RAFLYLAE R+DKRHR MA W+L +DMTVSEFLAADIDNNG+V
Sbjct: 361 RAFLYLAEMRIDKRHRAMANWVLSRDMTVSEFLAADIDNNGYV 393

BLAST of Spg012030 vs. ExPASy Swiss-Prot
Match: Q9S6Z8 (Two-pore potassium channel 5 OS=Arabidopsis thaliana OX=3702 GN=TPK5 PE=1 SV=1)

HSP 1 Score: 323.6 bits (828), Expect = 3.3e-87
Identity = 189/345 (54.78%), Postives = 235/345 (68.12%), Query Frame = 0

Query: 49  PSSSPQDGSPAFDALTLSLSSSRPSASSSLQDPSTPLDPKSQSQSQQGWPVDSNYSWHKS 108
           PSSS    S     +T+  S S  S    +      L  +   QS Q    D +    ++
Sbjct: 20  PSSSSSSAS-----ITIPRSISNTSFFHEISQERLLLHHQDLEQSVQDDKEDQDSDSDET 79

Query: 109 N--------LHRSKTAPAMAVINDVNHS--QEPKPQFGKQSIIRQAVVLLIIYLSLGVLI 168
           N        LHRS+TAPAM +I D+     +  KP    +SIIRQA+ LLI+YL+LGV I
Sbjct: 80  NRFLSQTRPLHRSRTAPAMVIIKDLRTKPPETKKPSPVSKSIIRQAIFLLIVYLTLGVSI 139

Query: 169 YWINRDKFSATETHPVVDALYFCIVTMCTIGYGDITPNSAATKLFSVFFVLVGFGFIDIL 228
           Y  NRD +S  ETHPVVDALYFCIVTMCTIGYGDI P +  TK+F+V FVL GFGF+DIL
Sbjct: 140 YSFNRDHYSGIETHPVVDALYFCIVTMCTIGYGDIAPLTPWTKIFAVVFVLFGFGFLDIL 199

Query: 229 LSGMVSYVLDLQESYLLRNVKRGVQQE-------SRKSYIIDVKKGRMRIRMKVALALGV 288
           LSG+V+YVLDLQES +L  ++     +       S K YIID +KGRMRIRMKV LAL V
Sbjct: 200 LSGVVNYVLDLQESMILTGIQTRQHHQHHHHHRFSAKDYIIDFEKGRMRIRMKVCLALCV 259

Query: 289 VILCIGIGMGVMHFVENLGWLDSFYLSVMSVTTVGYGDQAFKSMTGRIFASIWLLVSTLA 348
           V+LCIG+G  V+HFVE LG++DS YLSVMSVTTVGYGD+AFK++ GR+FA++WLLVSTLA
Sbjct: 260 VVLCIGVGALVLHFVEELGFVDSVYLSVMSVTTVGYGDRAFKTLQGRLFAAVWLLVSTLA 319

Query: 349 VARAFLYLAEARVDKRHRMMAKWILGQDMTVSEFLAADIDNNGFV 377
           VARAFLYLAEAR+D+RHR   K  L +++TV + L AD   +GF+
Sbjct: 320 VARAFLYLAEARIDRRHRKAVKLALNREITVDDLLKADTYQHGFI 359

BLAST of Spg012030 vs. ExPASy Swiss-Prot
Match: Q9FWX6 (Two-pore potassium channel 4 OS=Arabidopsis thaliana OX=3702 GN=TPK4 PE=2 SV=2)

HSP 1 Score: 190.3 bits (482), Expect = 4.3e-47
Identity = 106/237 (44.73%), Postives = 153/237 (64.56%), Query Frame = 0

Query: 110 LHRSKTAPAMAVINDVNHSQEPKPQFGKQSIIRQAVVLLIIYLSLGVLIYWINRDKFSAT 169
           LH ++++P    +  V+ S        K +I+  A++LL++YL+ GV  Y   RD+FS T
Sbjct: 12  LHPNESSPEETQVTTVSKS--------KWTILVLAMILLLVYLTFGVCTYSFFRDQFSGT 71

Query: 170 ETHPVVDALYFCIVTMCTIGYGDITPNSAATKLFSVFFVLVGFGFIDILLSGMVSYVLDL 229
           ET+  VDA YF IVT  T+GYGDI P+++ TK+ ++  V  G  F+D LL+ +VS+VL L
Sbjct: 72  ETNLFVDAFYFSIVTFSTVGYGDIVPSTSTTKILTIVLVSTGVVFLDYLLNRVVSHVLSL 131

Query: 230 QESYLLRNVKRGVQQESRKSYIIDVKKGRMRIRMKVALALGVVILCIGIGMGVMHFVENL 289
           QE+ +L  + +   +  R     D   G++R++ K+ LA   V LC+G G   +H  E L
Sbjct: 132 QENAILDRINKTRNRAIRDHIAED---GKIRLKWKLCLAFCAVGLCVGSGALFLHVFERL 191

Query: 290 GWLDSFYLSVMSVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDK 347
            WLDS YLSV+SVTTVGYGD+ FK++ GR FA  WLL+ST+A+A  FLYLAE R+D+
Sbjct: 192 DWLDSVYLSVISVTTVGYGDKTFKTVEGRGFAVFWLLLSTIAMATLFLYLAEMRIDR 237

BLAST of Spg012030 vs. ExPASy TrEMBL
Match: A0A5A7UFS5 (Two-pore potassium channel 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G008780 PE=3 SV=1)

HSP 1 Score: 696.0 bits (1795), Expect = 9.0e-197
Identity = 361/402 (89.80%), Postives = 373/402 (92.79%), Query Frame = 0

Query: 1   MEKEPLLPYLSPRGKPPPLPPQLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDGSPAF 60
           MEKEPLLPYLSPRGKPPP PPQLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQD SP F
Sbjct: 1   MEKEPLLPYLSPRGKPPPPPPQLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDASPIF 60

Query: 61  DALTLSLSSSRPSASSSLQDPSTPLDPKSQSQSQQGWPVDSNYSWHKSNLHRSKTAPAMA 120
           DALTLSLSSSRPSASSSLQDPSTPLDP+SQSQSQQ W VDSNY+W KSNLHRSKTAPAMA
Sbjct: 61  DALTLSLSSSRPSASSSLQDPSTPLDPRSQSQSQQAWLVDSNYTWPKSNLHRSKTAPAMA 120

Query: 121 VINDVNHSQEPKPQFGKQSIIRQAVVLLIIYLSLGVLIYWINRDKFSATETHPVVDALYF 180
           VINDVNHSQEPKPQFGKQSIIRQAVVLLI+YLSLGVLIYW+NRD F+A ETHPVVDALYF
Sbjct: 121 VINDVNHSQEPKPQFGKQSIIRQAVVLLIVYLSLGVLIYWLNRDNFAAQETHPVVDALYF 180

Query: 181 CIVTMCTIGYGDITPNSAATKLFSVFFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVKR 240
           CIVTMCTIGYGDITPNS ATKLFSV FVLVGFGFIDILLSGMVSYVLDLQESYLLRNVKR
Sbjct: 181 CIVTMCTIGYGDITPNSTATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVKR 240

Query: 241 GVQQESRKSYIIDVKKGRMRIRMKVALALGVVILCIGIGMGVMHFVENLGWLDSFYLSVM 300
           GV++ES KSYIIDVKKGRMRIRMKVALALGVVILCIG+GMGVMHFVENLGWLDSFYLSVM
Sbjct: 241 GVKRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGMGVMHFVENLGWLDSFYLSVM 300

Query: 301 SVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQDM 360
           SVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQDM
Sbjct: 301 SVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQDM 360

Query: 361 TVSEFLAADIDNNGFVRVHSKTMDFTEESVGIPVGAEVLQLD 403
           T+SEFLAADIDNNGFVR        +   V   +G +V   D
Sbjct: 361 TISEFLAADIDNNGFVRYALTLFIHSFREVAWKIGFDVTDSD 402

BLAST of Spg012030 vs. ExPASy TrEMBL
Match: A0A1S3AYW0 (two-pore potassium channel 3 OS=Cucumis melo OX=3656 GN=LOC103484391 PE=3 SV=1)

HSP 1 Score: 692.6 bits (1786), Expect = 9.9e-196
Identity = 356/376 (94.68%), Postives = 365/376 (97.07%), Query Frame = 0

Query: 1   MEKEPLLPYLSPRGKPPPLPPQLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDGSPAF 60
           MEKEPLLPYLSPRGKPPP PPQLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQD SP F
Sbjct: 1   MEKEPLLPYLSPRGKPPPPPPQLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDASPIF 60

Query: 61  DALTLSLSSSRPSASSSLQDPSTPLDPKSQSQSQQGWPVDSNYSWHKSNLHRSKTAPAMA 120
           DALTLSLSSSRPSASSSLQDPSTPLDP+SQSQSQQ W VDSNY+W KSNLHRSKTAPAMA
Sbjct: 61  DALTLSLSSSRPSASSSLQDPSTPLDPRSQSQSQQAWLVDSNYTWPKSNLHRSKTAPAMA 120

Query: 121 VINDVNHSQEPKPQFGKQSIIRQAVVLLIIYLSLGVLIYWINRDKFSATETHPVVDALYF 180
           VINDVNHSQEPKPQFGKQSIIRQAVVLLI+YLSLGVLIYW+NRD F+A ETHPVVDALYF
Sbjct: 121 VINDVNHSQEPKPQFGKQSIIRQAVVLLIVYLSLGVLIYWLNRDNFAAQETHPVVDALYF 180

Query: 181 CIVTMCTIGYGDITPNSAATKLFSVFFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVKR 240
           CIVTMCTIGYGDITPNS ATKLFSV FVLVGFGFIDILLSGMVSYVLDLQESYLLRNVKR
Sbjct: 181 CIVTMCTIGYGDITPNSTATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVKR 240

Query: 241 GVQQESRKSYIIDVKKGRMRIRMKVALALGVVILCIGIGMGVMHFVENLGWLDSFYLSVM 300
           GV++ES KSYIIDVKKGRMRIRMKVALALGVVILCIG+GMGVMHFVENLGWLDSFYLSVM
Sbjct: 241 GVKRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGMGVMHFVENLGWLDSFYLSVM 300

Query: 301 SVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQDM 360
           SVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQDM
Sbjct: 301 SVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQDM 360

Query: 361 TVSEFLAADIDNNGFV 377
           T+SEFLAADIDNNGFV
Sbjct: 361 TISEFLAADIDNNGFV 376

BLAST of Spg012030 vs. ExPASy TrEMBL
Match: A0A6J1ED61 (two-pore potassium channel 3-like OS=Cucurbita moschata OX=3662 GN=LOC111433251 PE=3 SV=1)

HSP 1 Score: 684.5 bits (1765), Expect = 2.7e-193
Identity = 359/377 (95.23%), Postives = 363/377 (96.29%), Query Frame = 0

Query: 1   MEKEPLLPYLSPRGKPPPLPPQ-LCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDGSPA 60
           MEKEPLLPY+SPRGKP P PPQ LCPLPENDEITLPMTPTEFKDRLIFGPSSSPQD SP 
Sbjct: 1   MEKEPLLPYISPRGKPLPPPPQLLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDTSPI 60

Query: 61  FDALTLSLSSSRPSASSSLQDPSTPLDPKSQSQSQQGWPVDSNYSWHKSNLHRSKTAPAM 120
           FDALTLSLSSSRPSASSSLQDPSTPL PKSQS SQQ W VDSNYSWHKSNLHRSKTAPAM
Sbjct: 61  FDALTLSLSSSRPSASSSLQDPSTPLAPKSQSPSQQAWLVDSNYSWHKSNLHRSKTAPAM 120

Query: 121 AVINDVNHSQEPKPQFGKQSIIRQAVVLLIIYLSLGVLIYWINRDKFSATETHPVVDALY 180
           AVINDVNHSQEPKPQFGKQSIIRQAVVLLI+YLSLGVLIYWINRDKFSA ETHPVVDALY
Sbjct: 121 AVINDVNHSQEPKPQFGKQSIIRQAVVLLILYLSLGVLIYWINRDKFSAHETHPVVDALY 180

Query: 181 FCIVTMCTIGYGDITPNSAATKLFSVFFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVK 240
           FCIVTMCTIGYGDITPNSAATKLFSV FVLVGFGFIDILLSGMVSYVLDLQESYLLRNVK
Sbjct: 181 FCIVTMCTIGYGDITPNSAATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVK 240

Query: 241 RGVQQESRKSYIIDVKKGRMRIRMKVALALGVVILCIGIGMGVMHFVENLGWLDSFYLSV 300
           RGVQ+ES KSYIIDVKKGRMRIRMKVALALGVVILCIG+GMGVMHFVENLGWLDSFYLSV
Sbjct: 241 RGVQRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGLGMGVMHFVENLGWLDSFYLSV 300

Query: 301 MSVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQD 360
           MSVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQD
Sbjct: 301 MSVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQD 360

Query: 361 MTVSEFLAADIDNNGFV 377
           MTVSEFLAAD DNNG V
Sbjct: 361 MTVSEFLAADTDNNGCV 377

BLAST of Spg012030 vs. ExPASy TrEMBL
Match: A0A6J1IME3 (two-pore potassium channel 3-like OS=Cucurbita maxima OX=3661 GN=LOC111478359 PE=3 SV=1)

HSP 1 Score: 681.8 bits (1758), Expect = 1.8e-192
Identity = 358/377 (94.96%), Postives = 362/377 (96.02%), Query Frame = 0

Query: 1   MEKEPLLPYLSPRGKPPPLPPQ-LCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDGSPA 60
           MEKEPLLPY+SPRGKP P PPQ LCPLPENDEITLPMTPTEFKDRLIFGPSSSPQD SP 
Sbjct: 1   MEKEPLLPYMSPRGKPLPPPPQLLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDTSPI 60

Query: 61  FDALTLSLSSSRPSASSSLQDPSTPLDPKSQSQSQQGWPVDSNYSWHKSNLHRSKTAPAM 120
           FDALTLSLSSSRPSASSSLQDPSTPL PKSQS SQQ W VDSNYSWHKSNLHRSKTAPAM
Sbjct: 61  FDALTLSLSSSRPSASSSLQDPSTPLAPKSQSPSQQAWLVDSNYSWHKSNLHRSKTAPAM 120

Query: 121 AVINDVNHSQEPKPQFGKQSIIRQAVVLLIIYLSLGVLIYWINRDKFSATETHPVVDALY 180
           AVINDVNHSQEPKPQFGK SIIRQAVVLLI+YLSLGVLIYWINRDKFSA ETHPVVDALY
Sbjct: 121 AVINDVNHSQEPKPQFGKLSIIRQAVVLLILYLSLGVLIYWINRDKFSAHETHPVVDALY 180

Query: 181 FCIVTMCTIGYGDITPNSAATKLFSVFFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVK 240
           FCIVTMCTIGYGDITPNSAATKLFSV FVLVGFGFIDILLSGMVSYVLDLQESYLLRNVK
Sbjct: 181 FCIVTMCTIGYGDITPNSAATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVK 240

Query: 241 RGVQQESRKSYIIDVKKGRMRIRMKVALALGVVILCIGIGMGVMHFVENLGWLDSFYLSV 300
           RGVQ+ES KSYIIDVKKGRMRIRMKVALALGVVILCIG+GMGVMHFVENLGWLDSFYLSV
Sbjct: 241 RGVQRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGLGMGVMHFVENLGWLDSFYLSV 300

Query: 301 MSVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQD 360
           MSVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQD
Sbjct: 301 MSVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQD 360

Query: 361 MTVSEFLAADIDNNGFV 377
           MTVSEFLAAD DNNG V
Sbjct: 361 MTVSEFLAADTDNNGCV 377

BLAST of Spg012030 vs. ExPASy TrEMBL
Match: A0A6J1KXM2 (two-pore potassium channel 3-like OS=Cucurbita maxima OX=3661 GN=LOC111497264 PE=3 SV=1)

HSP 1 Score: 666.8 bits (1719), Expect = 5.8e-188
Identity = 354/401 (88.28%), Postives = 370/401 (92.27%), Query Frame = 0

Query: 1   MEKEPLLPYLSPRGKPPPLPPQLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDGSPAF 60
           MEKEPLLPY+SPRGKPP L PQL PLPENDEI LPMTPTEFKDRLIFGPSSSPQD S AF
Sbjct: 1   MEKEPLLPYMSPRGKPPSL-PQLYPLPENDEIILPMTPTEFKDRLIFGPSSSPQDASSAF 60

Query: 61  DALTLSLSSSRPSASSSLQDPSTPLDPKSQSQSQQGWPVDSNYSWHKSNLHRSKTAPAMA 120
           DALTLSL+S RPSASSS QDPSTPLDPKSQS SQQGW VDS +S HKSNLHRSKTAPAMA
Sbjct: 61  DALTLSLASPRPSASSSRQDPSTPLDPKSQSPSQQGWLVDSKHSCHKSNLHRSKTAPAMA 120

Query: 121 VINDVNHSQEPKPQFGKQSIIRQAVVLLIIYLSLGVLIYWINRDKFSATETHPVVDALYF 180
           VIND+NHSQEPKPQFGKQSIIRQAVVLLIIYLSLGV+IYWINRD FSA+ETHPVVDALYF
Sbjct: 121 VINDINHSQEPKPQFGKQSIIRQAVVLLIIYLSLGVVIYWINRDNFSASETHPVVDALYF 180

Query: 181 CIVTMCTIGYGDITPNSAATKLFSVFFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVKR 240
           CIVTMCTIGYGDITPNSAATKLFSV FVLVGFGFIDILLSGMVSYVLDLQESYLLRNVKR
Sbjct: 181 CIVTMCTIGYGDITPNSAATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVKR 240

Query: 241 GVQQESRKSYIIDVKKGRMRIRMKVALALGVVILCIGIGMGVMHFVENLGWLDSFYLSVM 300
           GVQ+ES KSYIIDVKKGRMRIRMKVALALGVVILCIGIGMGVMHFVE+LGWLDSFYLSVM
Sbjct: 241 GVQRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGIGMGVMHFVEDLGWLDSFYLSVM 300

Query: 301 SVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQDM 360
           SVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILG DM
Sbjct: 301 SVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGHDM 360

Query: 361 TVSEFLAADIDNNGFVRVHSKTMDFTEESVGIPVGAEVLQL 402
           TVSEFLAAD DNNG V   S+ + +  + +G     +V+Q+
Sbjct: 361 TVSEFLAADTDNNGCVS-RSEYVVYKLKEMGKVTEKDVMQI 399

BLAST of Spg012030 vs. TAIR 10
Match: AT4G18160.1 (Ca2+ activated outward rectifying K+ channel 6 )

HSP 1 Score: 442.2 bits (1136), Expect = 4.5e-124
Identity = 255/414 (61.59%), Postives = 312/414 (75.36%), Query Frame = 0

Query: 4   EPLLPY-LSPRGKPPPLPPQLCPLPENDE--ITLPMTPTEFKDRLIFGP-SSSPQDGSPA 63
           +PLL Y +SPR K P  P  L PLPE++E  I +PMTP+EFK+RLIFGP S SP+D S  
Sbjct: 7   DPLLQYMISPRLKKP--PQLLFPLPEDNEVAIPMPMTPSEFKERLIFGPFSCSPRDSSHF 66

Query: 64  FDALTL-SLSSSRPSASSSLQDPST--PLDPKSQSQSQQGWPVDSNYSWHKSN-LHRSKT 123
            D++   S SSS  + ++   D ST  PL P    Q +  W  D   S  + + LHRSKT
Sbjct: 67  IDSMKQPSPSSSSTAVNNPFSDSSTLDPLLPPPPPQPEP-WLSDQTSSHCQGHALHRSKT 126

Query: 124 APAMAVINDVNHS-QEPKPQFGKQSIIRQAVVLLIIYLSLGVLIYWINRDKFSATETHPV 183
           APAMAVIND++H  ++  P    +S++RQA  LL++YLSLGVLIYW+NRD +   +THPV
Sbjct: 127 APAMAVINDLHHPIRQKDPTETSRSVVRQAFALLVVYLSLGVLIYWLNRDHYVVNQTHPV 186

Query: 184 VDALYFCIVTMCTIGYGDITPNSAATKLFSVFFVLVGFGFIDILLSGMVSYVLDLQESYL 243
           VD LYFCIVTMCTIGYGDITPNS  TKLFS+ FVLVGFGFIDILLSGMVSYVLDLQESY+
Sbjct: 187 VDGLYFCIVTMCTIGYGDITPNSVVTKLFSIMFVLVGFGFIDILLSGMVSYVLDLQESYM 246

Query: 244 LRNVKRGVQQESRKSYIIDVKKGRMRIRMKVALALGVVILCIGIGMGVMHFVENLGWLDS 303
           L + KR  + E R+SYIIDVKKGRMRIR+KVALALGVV+LCI +G+G+MHF+E +GWLDS
Sbjct: 247 LDSAKRRDEPEKRRSYIIDVKKGRMRIRLKVALALGVVVLCIAVGVGIMHFIEEIGWLDS 306

Query: 304 FYLSVMSVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKW 363
           FYLSVMSVTTVGYGD+AFK++ GR+FA+IWLLVSTLAVARAFLYLAEARVDKR+R  AK 
Sbjct: 307 FYLSVMSVTTVGYGDRAFKTLPGRLFAAIWLLVSTLAVARAFLYLAEARVDKRNRERAKK 366

Query: 364 ILGQDMTVSEFLAADIDNNG------FVRVHSKTMDFTEESVGIPVGAEVLQLD 403
           +L + M+VS+F AADIDNNG      +V    K M+   +   +P+  +  +LD
Sbjct: 367 VLCETMSVSQFFAADIDNNGCVSKAEYVIYKLKEMEKITDKDILPISKQFDKLD 417

BLAST of Spg012030 vs. TAIR 10
Match: AT5G46370.1 (Ca2+ activated outward rectifying K+ channel 2 )

HSP 1 Score: 406.8 bits (1044), Expect = 2.1e-113
Identity = 239/415 (57.59%), Postives = 301/415 (72.53%), Query Frame = 0

Query: 4   EPLLPYL-SPRGKPPPLPPQLCPLPENDEITLPM--TPTEFKDRLIFGP-SSSPQDGSPA 63
           +PL  YL +PR  PP  PP L  LPEN+++T+PM  TP E K+RLIFG    S ++ S  
Sbjct: 12  DPLRQYLMNPRINPP--PPSLLTLPENNDVTIPMPITPLELKNRLIFGSFVRSRKESSLP 71

Query: 64  FDALTLSLSSSRPSASSSLQDPSTPLDPKSQSQSQQGWPVDSNYSWHKSNLHRSKTAPAM 123
            DAL+ + S+S  SA++S  D +  L P ++       PV    S    N HRSKTAPAM
Sbjct: 72  IDALSQNPSTS-SSATTSFSDSTDLLLPLTEPNK----PV--RKSKPTINFHRSKTAPAM 131

Query: 124 AVINDVNHSQEPK--PQFGKQSIIRQAVVLLIIYLSLGVLIYWINRDKFSATETHPVVDA 183
           A IN+++H  +PK   Q   ++I+ QAV LL++YLSLGVLIYW+NRD ++  +THPVVDA
Sbjct: 132 AAINNISHPNDPKTDQQSDSKTIVNQAVALLVVYLSLGVLIYWLNRDSYNVKQTHPVVDA 191

Query: 184 LYFCIVTMCTIGYGDITPNSAATKLFSVFFVLVGFGFIDILLSGMVSYVLDLQESYLL-- 243
           LYFCIVTMCTIGYGDITP+S  TKLFS+FFVLVGFGF+DILLSGMV+YVLDLQE+Y+L  
Sbjct: 192 LYFCIVTMCTIGYGDITPDSVVTKLFSIFFVLVGFGFMDILLSGMVTYVLDLQENYMLET 251

Query: 244 -RNVKRGVQQESR-KSYIIDVKKGRMRIRMKVALALGVVILCIGIGMGVMHFVENLGWLD 303
            RN    +    + +SYIIDVKKGRMRIR+KV LALGVV+LC+G G+ +MHFVE +GWLD
Sbjct: 252 ARNESLNLNDRDKVRSYIIDVKKGRMRIRLKVGLALGVVVLCLGFGVLIMHFVEKIGWLD 311

Query: 304 SFYLSVMSVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAK 363
           SFY SVMSVTTVGYGD+AF ++ GR+ A++WLLVSTLAVARA L+LAE+RVDKR+R  AK
Sbjct: 312 SFYFSVMSVTTVGYGDRAFNTLAGRLLAAMWLLVSTLAVARAILFLAESRVDKRNRERAK 371

Query: 364 WILGQDMTVSEFLAADIDNNG------FVRVHSKTMDFTEESVGIPVGAEVLQLD 403
            +LG+ M++S+FL ADID NG      FV    K MD   E    P+G +  +LD
Sbjct: 372 KVLGESMSISQFLDADIDCNGCVSKAEFVIYKLKKMDKITEKDINPIGFQFDKLD 417

BLAST of Spg012030 vs. TAIR 10
Match: AT4G01840.1 (Ca2+ activated outward rectifying K+ channel 5 )

HSP 1 Score: 323.6 bits (828), Expect = 2.3e-88
Identity = 189/345 (54.78%), Postives = 235/345 (68.12%), Query Frame = 0

Query: 49  PSSSPQDGSPAFDALTLSLSSSRPSASSSLQDPSTPLDPKSQSQSQQGWPVDSNYSWHKS 108
           PSSS    S     +T+  S S  S    +      L  +   QS Q    D +    ++
Sbjct: 20  PSSSSSSAS-----ITIPRSISNTSFFHEISQERLLLHHQDLEQSVQDDKEDQDSDSDET 79

Query: 109 N--------LHRSKTAPAMAVINDVNHS--QEPKPQFGKQSIIRQAVVLLIIYLSLGVLI 168
           N        LHRS+TAPAM +I D+     +  KP    +SIIRQA+ LLI+YL+LGV I
Sbjct: 80  NRFLSQTRPLHRSRTAPAMVIIKDLRTKPPETKKPSPVSKSIIRQAIFLLIVYLTLGVSI 139

Query: 169 YWINRDKFSATETHPVVDALYFCIVTMCTIGYGDITPNSAATKLFSVFFVLVGFGFIDIL 228
           Y  NRD +S  ETHPVVDALYFCIVTMCTIGYGDI P +  TK+F+V FVL GFGF+DIL
Sbjct: 140 YSFNRDHYSGIETHPVVDALYFCIVTMCTIGYGDIAPLTPWTKIFAVVFVLFGFGFLDIL 199

Query: 229 LSGMVSYVLDLQESYLLRNVKRGVQQE-------SRKSYIIDVKKGRMRIRMKVALALGV 288
           LSG+V+YVLDLQES +L  ++     +       S K YIID +KGRMRIRMKV LAL V
Sbjct: 200 LSGVVNYVLDLQESMILTGIQTRQHHQHHHHHRFSAKDYIIDFEKGRMRIRMKVCLALCV 259

Query: 289 VILCIGIGMGVMHFVENLGWLDSFYLSVMSVTTVGYGDQAFKSMTGRIFASIWLLVSTLA 348
           V+LCIG+G  V+HFVE LG++DS YLSVMSVTTVGYGD+AFK++ GR+FA++WLLVSTLA
Sbjct: 260 VVLCIGVGALVLHFVEELGFVDSVYLSVMSVTTVGYGDRAFKTLQGRLFAAVWLLVSTLA 319

Query: 349 VARAFLYLAEARVDKRHRMMAKWILGQDMTVSEFLAADIDNNGFV 377
           VARAFLYLAEAR+D+RHR   K  L +++TV + L AD   +GF+
Sbjct: 320 VARAFLYLAEARIDRRHRKAVKLALNREITVDDLLKADTYQHGFI 359

BLAST of Spg012030 vs. TAIR 10
Match: AT1G02510.1 (Outward rectifying potassium channel protein )

HSP 1 Score: 190.3 bits (482), Expect = 3.1e-48
Identity = 106/237 (44.73%), Postives = 153/237 (64.56%), Query Frame = 0

Query: 110 LHRSKTAPAMAVINDVNHSQEPKPQFGKQSIIRQAVVLLIIYLSLGVLIYWINRDKFSAT 169
           LH ++++P    +  V+ S        K +I+  A++LL++YL+ GV  Y   RD+FS T
Sbjct: 12  LHPNESSPEETQVTTVSKS--------KWTILVLAMILLLVYLTFGVCTYSFFRDQFSGT 71

Query: 170 ETHPVVDALYFCIVTMCTIGYGDITPNSAATKLFSVFFVLVGFGFIDILLSGMVSYVLDL 229
           ET+  VDA YF IVT  T+GYGDI P+++ TK+ ++  V  G  F+D LL+ +VS+VL L
Sbjct: 72  ETNLFVDAFYFSIVTFSTVGYGDIVPSTSTTKILTIVLVSTGVVFLDYLLNRVVSHVLSL 131

Query: 230 QESYLLRNVKRGVQQESRKSYIIDVKKGRMRIRMKVALALGVVILCIGIGMGVMHFVENL 289
           QE+ +L  + +   +  R     D   G++R++ K+ LA   V LC+G G   +H  E L
Sbjct: 132 QENAILDRINKTRNRAIRDHIAED---GKIRLKWKLCLAFCAVGLCVGSGALFLHVFERL 191

Query: 290 GWLDSFYLSVMSVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDK 347
            WLDS YLSV+SVTTVGYGD+ FK++ GR FA  WLL+ST+A+A  FLYLAE R+D+
Sbjct: 192 DWLDSVYLSVISVTTVGYGDKTFKTVEGRGFAVFWLLLSTIAMATLFLYLAEMRIDR 237

BLAST of Spg012030 vs. TAIR 10
Match: AT5G55630.1 (Outward rectifying potassium channel protein )

HSP 1 Score: 164.5 bits (415), Expect = 1.8e-40
Identity = 106/304 (34.87%), Postives = 172/304 (56.58%), Query Frame = 0

Query: 83  TPLDPKSQSQSQ-QGWPVDS-NYSWHKSNLHRSKTAP-AMAVINDVNHSQEPKPQFGKQS 142
           TPL P  +  +  Q + ++S   S  K  L RS++AP    + ND     EP P   K  
Sbjct: 8   TPLLPTEKIDTMAQDFNLNSRTSSSRKRRLRRSRSAPRGDCMYNDDVKIDEPPPHPSKIP 67

Query: 143 I-------IRQAVVLLIIYLSLGVLIYWINRDKFSATETHPVVDALYFCIVTMCTIGYGD 202
           +       +R+ ++ L +YL++G L +++ RD+ S  +T  VVDALYFCIVTM T+GYGD
Sbjct: 68  MFSDLNPNLRRVIMFLALYLTIGTLCFYLVRDQISGHKTSGVVDALYFCIVTMTTVGYGD 127

Query: 203 ITPNSAATKLFSVFFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVKRGVQQESRKSYII 262
           + PNS+A++L +  FV  G   +  LLS    Y+++ QE+ L+R      Q       + 
Sbjct: 128 LVPNSSASRLLACAFVFSGMVLVGHLLSRAADYLVEKQEALLVRAFHLR-QSFGPTDILK 187

Query: 263 DVKKGRMRIRMKVALALGVVILCIGIGMGVMHFVENLGWLDSFYLSVMSVTTVGYGDQAF 322
           ++   ++R +      + VV+  +G    VM  VE +  + +FY    +VTT+GYGD++F
Sbjct: 188 ELHTNKLRYKCYATCLVLVVLFIVGTIFLVM--VEKMPVISAFYCVCSTVTTLGYGDKSF 247

Query: 323 KSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQDMTVSEFLAADIDN 377
            S  GR+FA  W+L S++ +A+ FLY+AE   + + R + KW+L + +T ++  AAD+D 
Sbjct: 248 NSEAGRLFAVFWILTSSICLAQFFLYVAELNTENKQRALVKWVLTRRITNNDLEAADLDE 307

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAA0052565.11.9e-19689.80two-pore potassium channel 3 [Cucumis melo var. makuwa] >TYK13260.1 two-pore pot... [more]
XP_008439665.12.1e-19594.68PREDICTED: two-pore potassium channel 3 [Cucumis melo][more]
KAE8647742.12.1e-19594.43hypothetical protein Csa_004248 [Cucumis sativus][more]
XP_004134597.17.8e-19594.41two-pore potassium channel 3 [Cucumis sativus][more]
XP_023544618.15.6e-19395.23two-pore potassium channel 3-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q9SVV66.3e-12361.59Two-pore potassium channel 3 OS=Arabidopsis thaliana OX=3702 GN=TPK3 PE=2 SV=1[more]
Q9FL252.9e-11257.59Two-pore potassium channel 2 OS=Arabidopsis thaliana OX=3702 GN=TPK2 PE=2 SV=1[more]
Q69TN41.4e-9352.11Two pore potassium channel c OS=Oryza sativa subsp. japonica OX=39947 GN=TPKC PE... [more]
Q9S6Z83.3e-8754.78Two-pore potassium channel 5 OS=Arabidopsis thaliana OX=3702 GN=TPK5 PE=1 SV=1[more]
Q9FWX64.3e-4744.73Two-pore potassium channel 4 OS=Arabidopsis thaliana OX=3702 GN=TPK4 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
A0A5A7UFS59.0e-19789.80Two-pore potassium channel 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca... [more]
A0A1S3AYW09.9e-19694.68two-pore potassium channel 3 OS=Cucumis melo OX=3656 GN=LOC103484391 PE=3 SV=1[more]
A0A6J1ED612.7e-19395.23two-pore potassium channel 3-like OS=Cucurbita moschata OX=3662 GN=LOC111433251 ... [more]
A0A6J1IME31.8e-19294.96two-pore potassium channel 3-like OS=Cucurbita maxima OX=3661 GN=LOC111478359 PE... [more]
A0A6J1KXM25.8e-18888.28two-pore potassium channel 3-like OS=Cucurbita maxima OX=3661 GN=LOC111497264 PE... [more]
Match NameE-valueIdentityDescription
AT4G18160.14.5e-12461.59Ca2+ activated outward rectifying K+ channel 6 [more]
AT5G46370.12.1e-11357.59Ca2+ activated outward rectifying K+ channel 2 [more]
AT4G01840.12.3e-8854.78Ca2+ activated outward rectifying K+ channel 5 [more]
AT1G02510.13.1e-4844.73Outward rectifying potassium channel protein [more]
AT5G55630.11.8e-4034.87Outward rectifying potassium channel protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003280Two pore domain potassium channelPRINTSPR013332POREKCHANELcoord: 303..312
score: 47.04
coord: 183..211
score: 36.02
IPR003280Two pore domain potassium channelPANTHERPTHR11003POTASSIUM CHANNEL, SUBFAMILY Kcoord: 90..377
NoneNo IPR availableGENE3D1.10.287.70coord: 137..234
e-value: 3.7E-21
score: 76.8
NoneNo IPR availableGENE3D1.10.287.70coord: 235..364
e-value: 1.8E-18
score: 68.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 10..25
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 50..98
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..98
NoneNo IPR availablePANTHERPTHR11003:SF282TWO-PORE POTASSIUM CHANNEL 3coord: 90..377
NoneNo IPR availableSUPERFAMILY81324Voltage-gated potassium channelscoord: 140..226
NoneNo IPR availableSUPERFAMILY81324Voltage-gated potassium channelscoord: 254..359
IPR013099Potassium channel domainPFAMPF07885Ion_trans_2coord: 272..340
e-value: 4.6E-11
score: 42.5
coord: 147..226
e-value: 4.4E-16
score: 58.6

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg012030.1Spg012030.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0071805 potassium ion transmembrane transport
biological_process GO:0030322 stabilization of membrane potential
cellular_component GO:0005887 integral component of plasma membrane
cellular_component GO:0009705 plant-type vacuole membrane
cellular_component GO:0016020 membrane
molecular_function GO:0022841 potassium ion leak channel activity
molecular_function GO:0005267 potassium channel activity