Spg012000 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg012000 vs. NCBI nr
Match: XP_023518482.1 (GDSL esterase/lipase At1g28570-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 476.9 bits (1226), Expect = 1.6e-130
Identity = 250/416 (60.10%), Postives = 279/416 (67.07%), Query Frame = 0

Query: 9   SSSILQLFIPLAFVIFVPCVFGCYTSIFNFGDSLSDTGNLYYTCSSPDPSQVCFFPYGET 68
           S  +L L I  AF   V CV G YTSIFNFGDSLSDTGNLYYTCSSP+P+ VCF PYGET
Sbjct: 4   SIPLLHLAIAFAFAFSVHCVVGRYTSIFNFGDSLSDTGNLYYTCSSPNPAHVCFLPYGET 63

Query: 69  FFHRPTGRFSDGRVIPDFFGLISSPIPRFLCFVLFPEIWVLSDFGVFAAISLRLPLLKPY 128
           FFHRPTGRFSDGRVI DF                              A SL LPL++PY
Sbjct: 64  FFHRPTGRFSDGRVILDFI-----------------------------ATSLGLPLVRPY 123

Query: 129 RS---GGEGISAEDFGNGLNFAVGGATALDVSFFRERGIHDLPSTAVSLRFQLQWFKQAY 188
           RS   GGEGISA+DFG GLNFAVGGATALDVS+F ERGIH++PS AVSLR QL WF + Y
Sbjct: 124 RSVGLGGEGISAQDFGKGLNFAVGGATALDVSYFEERGIHNVPSHAVSLRIQLHWFNKTY 183

Query: 189 SSFCASS----------------------------------------------------- 248
           SS CASS                                                     
Sbjct: 184 SSVCASSPSSRCRDIFKNSLFMMGEIGGNDYNYFFFDNRNITELTSLVPLVVKQIGSVIM 243

Query: 249 ---TLSVETLMVPANIPIGCLPVYLQLYETSNQDQYDPQNSCLKWLNRFSEYHNEQLHEE 308
               L V+T+MVP N+PIGCLP+YLQ+Y TSN+ QYDPQN CLKWLN+FS+YHNEQL +E
Sbjct: 244 ELIELGVDTIMVPGNLPIGCLPIYLQVYATSNRGQYDPQNGCLKWLNQFSKYHNEQLQQE 303

Query: 309 LKRIRALYPHVRLIYVDSFNAALRVYNAPKDFGFIDSREVCCVDGNGSYYFPLPCGLPST 366
           LKRIRAL+PHV LIY D FNA + + NAPK+FGF +  +VCCVD N SYYFP+PCGLPST
Sbjct: 304 LKRIRALHPHVHLIYADYFNAVMHILNAPKNFGFTNLHQVCCVDENRSYYFPMPCGLPST 363

BLAST of Spg012000 vs. NCBI nr
Match: KAG6594559.1 (GDSL esterase/lipase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 461.5 bits (1186), Expect = 7.0e-126
Identity = 244/413 (59.08%), Postives = 274/413 (66.34%), Query Frame = 0

Query: 11  SILQLFIPLAFVIFVPCVFGCYTSIFNFGDSLSDTGNLYYTCSSPDPSQVCFFPYGETFF 70
           SI  L +  AF   V  VFG YTSIFNFGDSLSDTGNLYYTCSSP+P+ VCF PYGETFF
Sbjct: 4   SIPLLHLAFAFAFSVHSVFGRYTSIFNFGDSLSDTGNLYYTCSSPNPAHVCFLPYGETFF 63

Query: 71  HRPTGRFSDGRVIPDFFGLISSPIPRFLCFVLFPEIWVLSDFGVFAAISLRLPLLKPYRS 130
           HRPTGRFSDGRVI DF                              A SL LPL++PYRS
Sbjct: 64  HRPTGRFSDGRVILDFI-----------------------------ATSLGLPLVRPYRS 123

Query: 131 ---GGEGISAEDFGNGLNFAVGGATALDVSFFRERGIHDLPSTAVSLRFQLQWFKQAYSS 190
              GGEGISAE+FG GLNFAVGGATALDVS+F ERGIH++PS AVSL  QLQWF + YSS
Sbjct: 124 VGMGGEGISAEEFGKGLNFAVGGATALDVSYFEERGIHNVPSHAVSLGIQLQWFNKTYSS 183

Query: 191 FCASS------------------------------------------------------- 250
            C+SS                                                       
Sbjct: 184 VCSSSPSSRCRDIFKNSLFMMGEIGGNDYNYFFFDNRNITELTSLVPLVVKQIGSVIMEL 243

Query: 251 -TLSVETLMVPANIPIGCLPVYLQLYETSNQDQYDPQNSCLKWLNRFSEYHNEQLHEELK 310
             L V+T+MVP N+PIGCLP+YLQ+Y TSN  QYDPQN CLKWLN+FS+YHN+QL +ELK
Sbjct: 244 IELGVDTIMVPGNLPIGCLPIYLQVYATSNGGQYDPQNGCLKWLNQFSKYHNKQLQQELK 303

Query: 311 RIRALYPHVRLIYVDSFNAALRVYNAPKDFGFIDSREVCCVDGNGSYYFPLPCGLPSTIV 365
            IRAL+PHV LIY D FNA + + NAPK+FGF +  +VCCVD N S+YFP+PCGLPSTIV
Sbjct: 304 LIRALHPHVHLIYADYFNALMDILNAPKNFGFTNLHQVCCVDENRSFYFPMPCGLPSTIV 363

BLAST of Spg012000 vs. NCBI nr
Match: XP_023004101.1 (GDSL esterase/lipase At1g28570-like [Cucurbita maxima])

HSP 1 Score: 454.5 bits (1168), Expect = 8.5e-124
Identity = 243/424 (57.31%), Postives = 273/424 (64.39%), Query Frame = 0

Query: 1   MASSKPSSSSSILQLFIPLAFVIFVPCVFGCYTSIFNFGDSLSDTGNLYYTCSSPDPSQV 60
           MA S PS   +    F   AF   +  VFG YTSIFNFGDSLSDTGNLYYTCSSP+P  V
Sbjct: 1   MAPSIPSLHLAFAFAF-AFAFAFSLHSVFGRYTSIFNFGDSLSDTGNLYYTCSSPNPVNV 60

Query: 61  CFFPYGETFFHRPTGRFSDGRVIPDFFGLISSPIPRFLCFVLFPEIWVLSDFGVFAAISL 120
           CF PYGETFFHRPTGRFSDGRV+ DF                              A  L
Sbjct: 61  CFLPYGETFFHRPTGRFSDGRVVLDFI-----------------------------ATWL 120

Query: 121 RLPLLKPYRS---GGEGISAEDFGNGLNFAVGGATALDVSFFRERGIHDLPSTAVSLRFQ 180
            LPL++PYR    GGEGISAE+FG GLNFAVGGATALD+SFF ERG+H++PS AVSL  Q
Sbjct: 121 GLPLVRPYRRVGLGGEGISAEEFGKGLNFAVGGATALDLSFFEERGVHNVPSHAVSLGIQ 180

Query: 181 LQWFKQAYSSFCASST-------------------------------------------- 240
           L W  + YSS CASS                                             
Sbjct: 181 LHWINKTYSSVCASSASSGCRDIFKNSLFILGEIGGNDYNYFLLDNRTITEVTSLVPLVV 240

Query: 241 ------------LSVETLMVPANIPIGCLPVYLQLYETSNQDQYDPQNSCLKWLNRFSEY 300
                       L V T+MVP N+P+GCLP+YLQ+Y TSN  QYDPQN CLKWLN+FS+Y
Sbjct: 241 KEISSAITELIELGVGTIMVPGNLPLGCLPIYLQVYATSNPSQYDPQNGCLKWLNQFSKY 300

Query: 301 HNEQLHEELKRIRALYPHVRLIYVDSFNAALRVYNAPKDFGFIDSREVCCVDGNGSYYFP 360
           HNEQL +EL+RIRAL+PHV LIY D FNA +R+ NAPK FGF +  +VCCVD N SYYFP
Sbjct: 301 HNEQLQQELERIRALHPHVHLIYADYFNAVMRILNAPKKFGFTNLHQVCCVDENRSYYFP 360

Query: 361 LPCGLPSTIVCDDPSKYVSWDGLHLTEAAYKVIATNLLQGSFTTPQFSISCLPQNSSTTL 366
           +PCGLPSTIVCDDPSKYVSWDGLHLTEAAYK+IAT+L+Q SFT PQFSI  L QNSST L
Sbjct: 361 MPCGLPSTIVCDDPSKYVSWDGLHLTEAAYKLIATSLMQASFTAPQFSI--LQQNSSTKL 392

BLAST of Spg012000 vs. NCBI nr
Match: TYK13195.1 (GDSL esterase/lipase [Cucumis melo var. makuwa])

HSP 1 Score: 445.3 bits (1144), Expect = 5.2e-121
Identity = 230/411 (55.96%), Postives = 270/411 (65.69%), Query Frame = 0

Query: 11  SILQLFIPLAFVIFVPCVFGCYTSIFNFGDSLSDTGNLYYTCSSPDPSQVCFFPYGETFF 70
           S + L +P   + F+P VFGCYTSIFNFGDSLSDTGNLYYTCS P+P  VCFFPYGETFF
Sbjct: 4   SFILLHLPF-LISFLPSVFGCYTSIFNFGDSLSDTGNLYYTCSFPNPPNVCFFPYGETFF 63

Query: 71  HRPTGRFSDGRVIPDFFGLISSPIPRFLCFVLFPEIWVLSDFGVFAAISLRLPLLKPYRS 130
           HRPTGRFSDGR++ DF                              A+SL LP ++PY+ 
Sbjct: 64  HRPTGRFSDGRLVLDFL-----------------------------AMSLGLPPVQPYQG 123

Query: 131 GGEGISAEDFGNGLNFAVGGATALDVSFFRERGIHDLPSTAVSLRFQLQWFKQAYSSFCA 190
             +GI+AEDF  GLNFAVGGATALD+SFF +RGI +LP T  SL  Q   F Q+YSS C 
Sbjct: 124 VEKGITAEDFQKGLNFAVGGATALDLSFFEQRGIPNLPPTVDSLGNQFNSFNQSYSSICT 183

Query: 191 SS-------------------------------------------------------TLS 250
           SS                                                        L 
Sbjct: 184 SSPPKCKDIFKSSLFIMGEIGGNDYNYLFFDGRNIEELESLVLLVVNKIASVILELIELG 243

Query: 251 VETLMVPANIPIGCLPVYLQLYE-TSNQDQYDPQNSCLKWLNRFSEYHNEQLHEELKRIR 310
           VETLMVP+N+PIGCLPVYL+ Y+ TSN+ Q+DPQN CLKWLN+FSEYHN+QL E+LKRIR
Sbjct: 244 VETLMVPSNLPIGCLPVYLEFYKTTSNESQFDPQNGCLKWLNQFSEYHNQQLQEQLKRIR 303

Query: 311 ALYPHVRLIYVDSFNAALRVYNAPKDFGFIDSREVCCVDGNGSYYFPLPCGLPSTIVCDD 366
            L+PHV LIYVD FNAA+R+YNAPKDFG ++  +VCCVD NGSYYFP PCG   TIVCDD
Sbjct: 304 VLHPHVHLIYVDYFNAAMRIYNAPKDFGLVEPLQVCCVDKNGSYYFPPPCGGAGTIVCDD 363

BLAST of Spg012000 vs. NCBI nr
Match: XP_008439761.1 (PREDICTED: GDSL esterase/lipase At1g28570-like [Cucumis melo])

HSP 1 Score: 444.9 bits (1143), Expect = 6.7e-121
Identity = 230/411 (55.96%), Postives = 270/411 (65.69%), Query Frame = 0

Query: 11  SILQLFIPLAFVIFVPCVFGCYTSIFNFGDSLSDTGNLYYTCSSPDPSQVCFFPYGETFF 70
           S + L +P   + F+P VFGCYTSIFNFGDSLSDTGNLYYTCS P+P  VCFFPYGETFF
Sbjct: 4   SFVLLHLPF-LISFLPSVFGCYTSIFNFGDSLSDTGNLYYTCSFPNPPNVCFFPYGETFF 63

Query: 71  HRPTGRFSDGRVIPDFFGLISSPIPRFLCFVLFPEIWVLSDFGVFAAISLRLPLLKPYRS 130
           HRPTGRFSDGR++ DF                              A+SL LP ++PY+ 
Sbjct: 64  HRPTGRFSDGRLVLDFL-----------------------------AMSLGLPPVQPYQG 123

Query: 131 GGEGISAEDFGNGLNFAVGGATALDVSFFRERGIHDLPSTAVSLRFQLQWFKQAYSSFCA 190
             +GI+AEDF  GLNFAVGGATALD+SFF +RGI +LP T  SL  Q   F Q+YSS C 
Sbjct: 124 VEKGITAEDFQKGLNFAVGGATALDLSFFEQRGIPNLPPTVDSLGNQFNSFNQSYSSICT 183

Query: 191 SS-------------------------------------------------------TLS 250
           SS                                                        L 
Sbjct: 184 SSPPKCKDIFKSSLFIMGEIGGNDYNYLFFDGRNIEELESLVLLVVNKIASVILELIELG 243

Query: 251 VETLMVPANIPIGCLPVYLQLYE-TSNQDQYDPQNSCLKWLNRFSEYHNEQLHEELKRIR 310
           VETLMVP+N+PIGCLPVYL+ Y+ TSN+ Q+DPQN CLKWLN+FSEYHN+QL E+LKRIR
Sbjct: 244 VETLMVPSNLPIGCLPVYLEFYKTTSNESQFDPQNGCLKWLNQFSEYHNQQLQEQLKRIR 303

Query: 311 ALYPHVRLIYVDSFNAALRVYNAPKDFGFIDSREVCCVDGNGSYYFPLPCGLPSTIVCDD 366
            L+PHV LIYVD FNAA+R+YNAPKDFG ++  +VCCVD NGSYYFP PCG   TIVCDD
Sbjct: 304 VLHPHVHLIYVDYFNAAMRIYNAPKDFGLVEPLQVCCVDKNGSYYFPPPCGGAGTIVCDD 363

BLAST of Spg012000 vs. ExPASy Swiss-Prot
Match: Q9FXJ1 (GDSL esterase/lipase At1g28570 OS=Arabidopsis thaliana OX=3702 GN=At1g28570 PE=3 SV=1)

HSP 1 Score: 254.6 bits (649), Expect = 1.7e-66
Identity = 152/385 (39.48%), Postives = 191/385 (49.61%), Query Frame = 0

Query: 32  YTSIFNFGDSLSDTGNLYYTCSSPDPSQVCFFPYGETFFHRPTGRFSDGRVIPDFFGLIS 91
           + SI +FGDS++DTGNL       +  +V F PYGETFFH PTGRFS+GR+I DF     
Sbjct: 32  FKSIISFGDSIADTGNLLALSDPTNLPKVAFLPYGETFFHHPTGRFSNGRLIIDF----- 91

Query: 92  SPIPRFLCFVLFPEIWVLSDFGVFAAISLRLPLLKPYRSGGEGISAEDFGNGLNFAVGGA 151
             I  FL F L P  +                          G    +F  G+NFAVGGA
Sbjct: 92  --IAEFLGFPLVPPFY--------------------------GSQNANFEKGVNFAVGGA 151

Query: 152 TALDVSFFRERGIHDLPSTAVSLRFQLQWFKQAYSSFCASST------------------ 211
           TAL+ SF  ERGIH  P T VSL  QL  FK++  + C S +                  
Sbjct: 152 TALERSFLEERGIH-FPYTNVSLAVQLSSFKESLPNLCVSPSDCRDMIENSLILMGEIGG 211

Query: 212 ---------------------LSVETL---------------MVPANIPIGCLPVYLQLY 271
                                L +ET+               +VP   P+GC   YL LY
Sbjct: 212 NDYNYAFFVGKNIEEIKELVPLVIETISSAITELIGMGGKTFLVPGEFPLGCSVAYLSLY 271

Query: 272 ETSNQDQYDPQNSCLKWLNRFSEYHNEQLHEELKRIRALYPHVRLIYVDSFNAALRVYNA 331
           +TSN ++YDP   CLKWLN+FSEYH+EQL  EL R++ LYPHV +IY D +N  LR+   
Sbjct: 272 QTSNIEEYDPLTGCLKWLNKFSEYHDEQLQAELNRLQKLYPHVNIIYADYYNTLLRLAQE 331

Query: 332 PKDFGFIDSREVCCVDGNGSYYFPL--PCGLPSTIVCDDPSKYVSWDGLHLTEAAYKVIA 361
           P  FGFI      C    G + F L    G      CDDPSKYVSWDG+H+TEAAY+++A
Sbjct: 332 PAKFGFISRPLPACCALGGPFNFTLGRKRGTQVPECCDDPSKYVSWDGVHMTEAAYRLMA 382

BLAST of Spg012000 vs. ExPASy Swiss-Prot
Match: Q9SHP6 (GDSL esterase/lipase At1g28610 OS=Arabidopsis thaliana OX=3702 GN=At1g28610 PE=2 SV=2)

HSP 1 Score: 249.2 bits (635), Expect = 7.1e-65
Identity = 152/404 (37.62%), Postives = 195/404 (48.27%), Query Frame = 0

Query: 7   SSSSSILQLFIPLAFVIFVPCVFGC--YTSIFNFGDSLSDTGNLYYTCSSPDPSQVCFFP 66
           +S  S++  F+   FV  V     C    SI +FGDS++DTGNL             F P
Sbjct: 2   ASLDSLVSFFLSTLFVTIVSSQTQCRNLESIISFGDSITDTGNLVGLSDRNHLPVTAFLP 61

Query: 67  YGETFFHRPTGRFSDGRVIPDFFGLISSPIPRFLCFVLFPEIWVLSDFGVFAAISLRLPL 126
           YGETFFH PTGR  +GR+I DF                      +++F       L LP 
Sbjct: 62  YGETFFHHPTGRSCNGRIIIDF----------------------IAEF-------LGLPH 121

Query: 127 LKPYRSGGEGISAEDFGNGLNFAVGGATALDVSFFRERGIHDLPSTAVSLRFQLQWFKQA 186
           + P+     G    +F  G+NFAV GATAL+ S   +RGI+  P + +SL  QL+ FK++
Sbjct: 122 VPPFYGSKNG----NFEKGVNFAVAGATALETSILEKRGIY-YPHSNISLGIQLKTFKES 181

Query: 187 YSSFCASST--------------------------------------------------- 246
             + C S T                                                   
Sbjct: 182 LPNLCGSPTDCRDMIGNAFIIMGEIGGNDFNFAFFVNKTSEVKELVPLVITKISSAIVEL 241

Query: 247 --LSVETLMVPANIPIGCLPVYLQLYETSNQDQYDPQNSCLKWLNRFSEYHNEQLHEELK 306
             +   T +VP N P+GC   YL LY+TSN+++YDP   CL WLN FSEY+NE+L  EL 
Sbjct: 242 VDMGGRTFLVPGNFPLGCSATYLTLYQTSNKEEYDPLTGCLTWLNDFSEYYNEKLQAELN 301

Query: 307 RIRALYPHVRLIYVDSFNAALRVYNAPKDFGFIDSREVCCVDGNGSYYFPL--PCGLPST 354
           R+  LYPHV +IY D FNA LR+Y  P  FGF+D     C    G Y F L   CG    
Sbjct: 302 RLSKLYPHVNIIYGDYFNALLRLYQEPSKFGFMDRPLPACCGLGGPYNFTLSKKCGSVGV 361

BLAST of Spg012000 vs. ExPASy Swiss-Prot
Match: Q94F40 (GDSL esterase/lipase At1g28600 OS=Arabidopsis thaliana OX=3702 GN=At1g28600 PE=2 SV=1)

HSP 1 Score: 247.7 bits (631), Expect = 2.1e-64
Identity = 154/405 (38.02%), Postives = 199/405 (49.14%), Query Frame = 0

Query: 7   SSSSSILQLFIPLAFVIFVPCVFGC--YTSIFNFGDSLSDTGNLYYTCSSPDPSQVCFFP 66
           S  S ++ LF  L FV  V     C  + SI +FGDS++DTGNL             F P
Sbjct: 3   SLDSLVIFLFSTL-FVTIVSSETPCPNFKSIISFGDSIADTGNLVGLSDRNQLPVTAFPP 62

Query: 67  YGETFFHRPTGRFSDGRVIPDFFGLISSPIPRFLCFVLFPEIWVLSDFGVFAAISLRLPL 126
           YGETFFH PTGR  DGR+I DF                      +++F       + LP 
Sbjct: 63  YGETFFHHPTGRSCDGRIIMDF----------------------IAEF-------VGLPY 122

Query: 127 LKPYRSGGEGISAEDFGNGLNFAVGGATALDVSFFRERGIHDLPSTAVSLRFQLQWFKQA 186
           + PY     G    +F  G+NFAV GATAL  SF ++RGI   P T VSL  QL+ FK++
Sbjct: 123 VPPY----FGSKNRNFDKGVNFAVAGATALKSSFLKKRGIQ--PHTNVSLGVQLKSFKKS 182

Query: 187 YSSFCASST--------------------------------------------------- 246
             + C S +                                                   
Sbjct: 183 LPNLCGSPSDCRDMIGNALILMGEIGGNDYNFPFFNRKPVKEVEELVPFVIASISSTITE 242

Query: 247 ---LSVETLMVPANIPIGCLPVYLQLYETSNQDQYDPQNSCLKWLNRFSEYHNEQLHEEL 306
              +  +T +VP   PIGC  VYL LY+TSN+D+YDP   CLKWLN+F EYH+E+L  EL
Sbjct: 243 LIGMGGKTFLVPGEFPIGCSVVYLTLYKTSNKDEYDPSTGCLKWLNKFGEYHSEKLKVEL 302

Query: 307 KRIRALYPHVRLIYVDSFNAALRVYNAPKDFGFIDSR-EVCC-VDGNGSYYFPLPCGLPS 354
            R+R LYPHV +IY D +N+ LR++  P  FGF++     CC + G  ++ F   CG   
Sbjct: 303 NRLRKLYPHVNIIYADYYNSLLRIFKEPAKFGFMERPFPACCGIGGPYNFNFTRKCGSVG 362

BLAST of Spg012000 vs. ExPASy Swiss-Prot
Match: Q9FXJ2 (GDSL esterase/lipase At1g28580 OS=Arabidopsis thaliana OX=3702 GN=At1g28580 PE=2 SV=1)

HSP 1 Score: 246.9 bits (629), Expect = 3.5e-64
Identity = 141/377 (37.40%), Postives = 186/377 (49.34%), Query Frame = 0

Query: 32  YTSIFNFGDSLSDTGNLYYTCSSPDPSQVCFFPYGETFFHRPTGRFSDGRVIPDFFGLIS 91
           + SI +FGDS++DTGNL       D   + F PYGE FFH PTGRFS+GR+I DF     
Sbjct: 35  FKSIISFGDSIADTGNLLGLSDPKDLPHMAFPPYGENFFHHPTGRFSNGRLIIDF----- 94

Query: 92  SPIPRFLCFVLFPEIWVLSDFGVFAAISLRLPLLKPYRSGGEGISAEDFGNGLNFAVGGA 151
                            +++F       L LPL+ P+     G    +F  G+NFAVGGA
Sbjct: 95  -----------------IAEF-------LGLPLVPPF----YGSHNANFEKGVNFAVGGA 154

Query: 152 TALDVSFFRERGIHDLPSTAVSLRFQLQWFKQAYSSFCASST------------------ 211
           TAL+ SF  +RGIH  P T VSL  QL  FK++  S C S +                  
Sbjct: 155 TALERSFLEDRGIH-FPYTNVSLGVQLNSFKESLPSICGSPSDCRDMIENALILMGEIGG 214

Query: 212 ------------------------------------LSVETLMVPANIPIGCLPVYLQLY 271
                                               +   T +VP   P+GC  +YL  +
Sbjct: 215 NDYNYAFFVDKGIEEIKELMPLVITTISSAITELIGMGGRTFLVPGEFPVGCSVLYLTSH 274

Query: 272 ETSNQDQYDPQNSCLKWLNRFSEYHNEQLHEELKRIRALYPHVRLIYVDSFNAALRVYNA 331
           +TSN ++YDP   CLKWLN+F E H EQL  EL R++ LYPHV +IY D +NA   +Y  
Sbjct: 275 QTSNMEEYDPLTGCLKWLNKFGENHGEQLRAELNRLQKLYPHVNIIYADYYNALFHLYQE 334

Query: 332 PKDFGFIDSREVCCVDGNGSYYFPL--PCGLPSTIVCDDPSKYVSWDGLHLTEAAYKVIA 353
           P  FGF++     C    G Y + +   CG      CDDPSKYV+WDG+H+TEAAY+++A
Sbjct: 335 PAKFGFMNRPLSACCGAGGPYNYTVGRKCGTDIVESCDDPSKYVAWDGVHMTEAAYRLMA 377

BLAST of Spg012000 vs. ExPASy Swiss-Prot
Match: Q9ZQI3 (GDSL esterase/lipase At2g27360 OS=Arabidopsis thaliana OX=3702 GN=At2g27360 PE=2 SV=1)

HSP 1 Score: 244.2 bits (622), Expect = 2.3e-63
Identity = 149/401 (37.16%), Postives = 196/401 (48.88%), Query Frame = 0

Query: 12  ILQLFIPLAFVIFVPCVFGC--YTSIFNFGDSLSDTGNLYYTCSSPDPSQVCFFPYGETF 71
           +L  FI    +  V     C  + SI +FGDS++DTGNL    S  D  +  F PYGETF
Sbjct: 9   LLSFFISTFLITVVTSQTRCRNFKSIISFGDSITDTGNLLGLSSPNDLPESAFPPYGETF 68

Query: 72  FHRPTGRFSDGRVIPDFFGLISSPIPRFLCFVLFPEIWVLSDFGVFAAISLRLPLLKPYR 131
           FH P+GRFSDGR+I DF                      +++F       L +P + P+ 
Sbjct: 69  FHHPSGRFSDGRLIIDF----------------------IAEF-------LGIPHVPPFY 128

Query: 132 SGGEGISAEDFGNGLNFAVGGATALDVSFFRERGIHDLPSTAVSLRFQLQWFKQAYSSFC 191
               G    +F  G+NFAVGGATAL+ S   E+G H   S  +SL  QL+ FK++    C
Sbjct: 129 GSKNG----NFEKGVNFAVGGATALECSVLEEKGTHCSQSN-ISLGNQLKSFKESLPYLC 188

Query: 192 ASST-------------------------------------------------------L 251
            SS+                                                       +
Sbjct: 189 GSSSPDCRDMIENAFILIGEIGGNDYNFPLFDRKNIEEVKELVPLVITTISSAISELVDM 248

Query: 252 SVETLMVPANIPIGCLPVYLQLYETSNQDQYDPQNSCLKWLNRFSEYHNEQLHEELKRIR 311
              T +VP N P+GC   YL LYET N+++Y+P   CL WLN FS YHNEQL  ELKR+R
Sbjct: 249 GARTFLVPGNFPLGCSVAYLTLYETPNKEEYNPLTGCLTWLNDFSVYHNEQLQAELKRLR 308

Query: 312 ALYPHVRLIYVDSFNAALRVYNAPKDFGFID-SREVCC-VDGNGSYYFPLPCGLPSTIVC 354
            LYPHV +IY D +N  LR+   P  FG +D     CC + G  ++ F + CG      C
Sbjct: 309 NLYPHVNIIYGDYYNTLLRLMQEPSKFGLMDRPLPACCGLGGPYNFTFSIKCGSKGVEYC 368

BLAST of Spg012000 vs. ExPASy TrEMBL
Match: A0A6J1KR60 (GDSL esterase/lipase At1g28570-like OS=Cucurbita maxima OX=3661 GN=LOC111497512 PE=3 SV=1)

HSP 1 Score: 454.5 bits (1168), Expect = 4.1e-124
Identity = 243/424 (57.31%), Postives = 273/424 (64.39%), Query Frame = 0

Query: 1   MASSKPSSSSSILQLFIPLAFVIFVPCVFGCYTSIFNFGDSLSDTGNLYYTCSSPDPSQV 60
           MA S PS   +    F   AF   +  VFG YTSIFNFGDSLSDTGNLYYTCSSP+P  V
Sbjct: 1   MAPSIPSLHLAFAFAF-AFAFAFSLHSVFGRYTSIFNFGDSLSDTGNLYYTCSSPNPVNV 60

Query: 61  CFFPYGETFFHRPTGRFSDGRVIPDFFGLISSPIPRFLCFVLFPEIWVLSDFGVFAAISL 120
           CF PYGETFFHRPTGRFSDGRV+ DF                              A  L
Sbjct: 61  CFLPYGETFFHRPTGRFSDGRVVLDFI-----------------------------ATWL 120

Query: 121 RLPLLKPYRS---GGEGISAEDFGNGLNFAVGGATALDVSFFRERGIHDLPSTAVSLRFQ 180
            LPL++PYR    GGEGISAE+FG GLNFAVGGATALD+SFF ERG+H++PS AVSL  Q
Sbjct: 121 GLPLVRPYRRVGLGGEGISAEEFGKGLNFAVGGATALDLSFFEERGVHNVPSHAVSLGIQ 180

Query: 181 LQWFKQAYSSFCASST-------------------------------------------- 240
           L W  + YSS CASS                                             
Sbjct: 181 LHWINKTYSSVCASSASSGCRDIFKNSLFILGEIGGNDYNYFLLDNRTITEVTSLVPLVV 240

Query: 241 ------------LSVETLMVPANIPIGCLPVYLQLYETSNQDQYDPQNSCLKWLNRFSEY 300
                       L V T+MVP N+P+GCLP+YLQ+Y TSN  QYDPQN CLKWLN+FS+Y
Sbjct: 241 KEISSAITELIELGVGTIMVPGNLPLGCLPIYLQVYATSNPSQYDPQNGCLKWLNQFSKY 300

Query: 301 HNEQLHEELKRIRALYPHVRLIYVDSFNAALRVYNAPKDFGFIDSREVCCVDGNGSYYFP 360
           HNEQL +EL+RIRAL+PHV LIY D FNA +R+ NAPK FGF +  +VCCVD N SYYFP
Sbjct: 301 HNEQLQQELERIRALHPHVHLIYADYFNAVMRILNAPKKFGFTNLHQVCCVDENRSYYFP 360

Query: 361 LPCGLPSTIVCDDPSKYVSWDGLHLTEAAYKVIATNLLQGSFTTPQFSISCLPQNSSTTL 366
           +PCGLPSTIVCDDPSKYVSWDGLHLTEAAYK+IAT+L+Q SFT PQFSI  L QNSST L
Sbjct: 361 MPCGLPSTIVCDDPSKYVSWDGLHLTEAAYKLIATSLMQASFTAPQFSI--LQQNSSTKL 392

BLAST of Spg012000 vs. ExPASy TrEMBL
Match: A0A5D3CNP6 (GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G008070 PE=3 SV=1)

HSP 1 Score: 445.3 bits (1144), Expect = 2.5e-121
Identity = 230/411 (55.96%), Postives = 270/411 (65.69%), Query Frame = 0

Query: 11  SILQLFIPLAFVIFVPCVFGCYTSIFNFGDSLSDTGNLYYTCSSPDPSQVCFFPYGETFF 70
           S + L +P   + F+P VFGCYTSIFNFGDSLSDTGNLYYTCS P+P  VCFFPYGETFF
Sbjct: 4   SFILLHLPF-LISFLPSVFGCYTSIFNFGDSLSDTGNLYYTCSFPNPPNVCFFPYGETFF 63

Query: 71  HRPTGRFSDGRVIPDFFGLISSPIPRFLCFVLFPEIWVLSDFGVFAAISLRLPLLKPYRS 130
           HRPTGRFSDGR++ DF                              A+SL LP ++PY+ 
Sbjct: 64  HRPTGRFSDGRLVLDFL-----------------------------AMSLGLPPVQPYQG 123

Query: 131 GGEGISAEDFGNGLNFAVGGATALDVSFFRERGIHDLPSTAVSLRFQLQWFKQAYSSFCA 190
             +GI+AEDF  GLNFAVGGATALD+SFF +RGI +LP T  SL  Q   F Q+YSS C 
Sbjct: 124 VEKGITAEDFQKGLNFAVGGATALDLSFFEQRGIPNLPPTVDSLGNQFNSFNQSYSSICT 183

Query: 191 SS-------------------------------------------------------TLS 250
           SS                                                        L 
Sbjct: 184 SSPPKCKDIFKSSLFIMGEIGGNDYNYLFFDGRNIEELESLVLLVVNKIASVILELIELG 243

Query: 251 VETLMVPANIPIGCLPVYLQLYE-TSNQDQYDPQNSCLKWLNRFSEYHNEQLHEELKRIR 310
           VETLMVP+N+PIGCLPVYL+ Y+ TSN+ Q+DPQN CLKWLN+FSEYHN+QL E+LKRIR
Sbjct: 244 VETLMVPSNLPIGCLPVYLEFYKTTSNESQFDPQNGCLKWLNQFSEYHNQQLQEQLKRIR 303

Query: 311 ALYPHVRLIYVDSFNAALRVYNAPKDFGFIDSREVCCVDGNGSYYFPLPCGLPSTIVCDD 366
            L+PHV LIYVD FNAA+R+YNAPKDFG ++  +VCCVD NGSYYFP PCG   TIVCDD
Sbjct: 304 VLHPHVHLIYVDYFNAAMRIYNAPKDFGLVEPLQVCCVDKNGSYYFPPPCGGAGTIVCDD 363

BLAST of Spg012000 vs. ExPASy TrEMBL
Match: A0A1S3AZJ0 (GDSL esterase/lipase At1g28570-like OS=Cucumis melo OX=3656 GN=LOC103484462 PE=3 SV=1)

HSP 1 Score: 444.9 bits (1143), Expect = 3.3e-121
Identity = 230/411 (55.96%), Postives = 270/411 (65.69%), Query Frame = 0

Query: 11  SILQLFIPLAFVIFVPCVFGCYTSIFNFGDSLSDTGNLYYTCSSPDPSQVCFFPYGETFF 70
           S + L +P   + F+P VFGCYTSIFNFGDSLSDTGNLYYTCS P+P  VCFFPYGETFF
Sbjct: 4   SFVLLHLPF-LISFLPSVFGCYTSIFNFGDSLSDTGNLYYTCSFPNPPNVCFFPYGETFF 63

Query: 71  HRPTGRFSDGRVIPDFFGLISSPIPRFLCFVLFPEIWVLSDFGVFAAISLRLPLLKPYRS 130
           HRPTGRFSDGR++ DF                              A+SL LP ++PY+ 
Sbjct: 64  HRPTGRFSDGRLVLDFL-----------------------------AMSLGLPPVQPYQG 123

Query: 131 GGEGISAEDFGNGLNFAVGGATALDVSFFRERGIHDLPSTAVSLRFQLQWFKQAYSSFCA 190
             +GI+AEDF  GLNFAVGGATALD+SFF +RGI +LP T  SL  Q   F Q+YSS C 
Sbjct: 124 VEKGITAEDFQKGLNFAVGGATALDLSFFEQRGIPNLPPTVDSLGNQFNSFNQSYSSICT 183

Query: 191 SS-------------------------------------------------------TLS 250
           SS                                                        L 
Sbjct: 184 SSPPKCKDIFKSSLFIMGEIGGNDYNYLFFDGRNIEELESLVLLVVNKIASVILELIELG 243

Query: 251 VETLMVPANIPIGCLPVYLQLYE-TSNQDQYDPQNSCLKWLNRFSEYHNEQLHEELKRIR 310
           VETLMVP+N+PIGCLPVYL+ Y+ TSN+ Q+DPQN CLKWLN+FSEYHN+QL E+LKRIR
Sbjct: 244 VETLMVPSNLPIGCLPVYLEFYKTTSNESQFDPQNGCLKWLNQFSEYHNQQLQEQLKRIR 303

Query: 311 ALYPHVRLIYVDSFNAALRVYNAPKDFGFIDSREVCCVDGNGSYYFPLPCGLPSTIVCDD 366
            L+PHV LIYVD FNAA+R+YNAPKDFG ++  +VCCVD NGSYYFP PCG   TIVCDD
Sbjct: 304 VLHPHVHLIYVDYFNAAMRIYNAPKDFGLVEPLQVCCVDKNGSYYFPPPCGGAGTIVCDD 363

BLAST of Spg012000 vs. ExPASy TrEMBL
Match: A0A6J1EFN7 (GDSL esterase/lipase At1g28570-like OS=Cucurbita moschata OX=3662 GN=LOC111433889 PE=3 SV=1)

HSP 1 Score: 424.5 bits (1090), Expect = 4.6e-115
Identity = 223/412 (54.13%), Postives = 265/412 (64.32%), Query Frame = 0

Query: 1   MASSKPSSSSSILQ---LFIPLAFVIFVPCVFGCYTSIFNFGDSLSDTGNLYYTCSSPDP 60
           M+ + PS S S+     L +  AF   V  VFG YTSIFNFGDSLSDTGNLYYTCSSP+P
Sbjct: 1   MSPTIPSFSFSMTPPSLLHLAFAFAFAVHSVFGRYTSIFNFGDSLSDTGNLYYTCSSPNP 60

Query: 61  SQVCFFPYGETFFHRPTGRFSDGRVIPDFFGLISSPIPRFLCFVLFPEIWVLSDFGVFAA 120
             VCF PYGETFFHRPTGRFSDGRVI DF                              A
Sbjct: 61  VHVCFLPYGETFFHRPTGRFSDGRVILDFI-----------------------------A 120

Query: 121 ISLRLPLLKPYRS---GGEGISAEDFGNGLNFAVGGATALDVSFFRERGIHDLPSTAVSL 180
            SL LPL++PYRS   G EGISAE+F  GLNFAVGGATAL++S+F ERG+H++PS AVSL
Sbjct: 121 TSLGLPLVRPYRSVGLGEEGISAEEFRKGLNFAVGGATALNLSYFEERGVHNVPSHAVSL 180

Query: 181 RFQLQWFKQAYSSFCASST----------------------------------------- 240
             QL WFK+ YSS CASS                                          
Sbjct: 181 EIQLDWFKKTYSSVCASSASSGCKDMFKKSLFIMGEIGGNDYNDFLFDKRNIEEVASLVP 240

Query: 241 ---------------LSVETLMVPANIPIGCLPVYLQLYETSNQDQYDPQNSCLKWLNRF 300
                          L V+T+MVP N PIGC+P+YL+L++TSN + YD +N CLKWLN+F
Sbjct: 241 LVIKQIGSAIRELIELGVDTIMVPGNFPIGCVPMYLKLFKTSNGEHYDSKNGCLKWLNQF 300

Query: 301 SEYHNEQLHEELKRIRALYPHVRLIYVDSFNAALRVYNAPKDFGFIDSREVCCVDGNGSY 351
           SEYHNEQL +EL+RI AL+PHV LIY D FNAA+R++NAPK+FGF+D  +VCCVDGN + 
Sbjct: 301 SEYHNEQLQQELERIGALHPHVHLIYADYFNAAMRLFNAPKNFGFVDIEQVCCVDGNRTI 360

BLAST of Spg012000 vs. ExPASy TrEMBL
Match: A0A5A7UA55 (GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold120G002420 PE=3 SV=1)

HSP 1 Score: 338.6 bits (867), Expect = 3.3e-89
Identity = 172/304 (56.58%), Postives = 205/304 (67.43%), Query Frame = 0

Query: 118 ISLRLPLLKPYRSGGEGISAEDFGNGLNFAVGGATALDVSFFRERGIHDLPSTAVSLRFQ 177
           +SL LP ++PY+   +GI+AEDF  GLNFAVGGATALD+SFF +RGI +LP T  SL  Q
Sbjct: 1   MSLGLPPVQPYQGVEKGITAEDFQKGLNFAVGGATALDLSFFEQRGIPNLPPTVDSLGNQ 60

Query: 178 LQWFKQAYSSFCASS--------------------------------------------- 237
              F Q+YSS C SS                                             
Sbjct: 61  FNSFNQSYSSICTSSPPKCKDIFKSSLFIMGEIGGNDYNYLFFDGRNIEELESLVLLVVN 120

Query: 238 ----------TLSVETLMVPANIPIGCLPVYLQLYE-TSNQDQYDPQNSCLKWLNRFSEY 297
                      L VETLMVP+N+PIGCLPVYL+ Y+ TSN+ Q+DPQN CLKWLN+FSEY
Sbjct: 121 KIASVILELIELGVETLMVPSNLPIGCLPVYLEFYKTTSNESQFDPQNGCLKWLNQFSEY 180

Query: 298 HNEQLHEELKRIRALYPHVRLIYVDSFNAALRVYNAPKDFGFIDSREVCCVDGNGSYYFP 357
           HN+QL E+LKRIR L+PHV LIYVD FNAA+R+YNAPKDFG ++  +VCCVD NGSYYFP
Sbjct: 181 HNQQLQEQLKRIRVLHPHVHLIYVDYFNAAMRIYNAPKDFGLVEPLQVCCVDKNGSYYFP 240

Query: 358 LPCGLPSTIVCDDPSKYVSWDGLHLTEAAYKVIATNLLQGSFTTPQFSISCLPQNSSTTL 366
            PCG   TIVCDDPSKY SWDGLHLTEAAY+++AT+++ G FT PQFSISCL +N+ST L
Sbjct: 241 PPCGGAGTIVCDDPSKYASWDGLHLTEAAYELMATSIINGEFTFPQFSISCLQRNTSTQL 300

BLAST of Spg012000 vs. TAIR 10
Match: AT1G28570.1 (SGNH hydrolase-type esterase superfamily protein )

HSP 1 Score: 254.6 bits (649), Expect = 1.2e-67
Identity = 152/385 (39.48%), Postives = 191/385 (49.61%), Query Frame = 0

Query: 32  YTSIFNFGDSLSDTGNLYYTCSSPDPSQVCFFPYGETFFHRPTGRFSDGRVIPDFFGLIS 91
           + SI +FGDS++DTGNL       +  +V F PYGETFFH PTGRFS+GR+I DF     
Sbjct: 32  FKSIISFGDSIADTGNLLALSDPTNLPKVAFLPYGETFFHHPTGRFSNGRLIIDF----- 91

Query: 92  SPIPRFLCFVLFPEIWVLSDFGVFAAISLRLPLLKPYRSGGEGISAEDFGNGLNFAVGGA 151
             I  FL F L P  +                          G    +F  G+NFAVGGA
Sbjct: 92  --IAEFLGFPLVPPFY--------------------------GSQNANFEKGVNFAVGGA 151

Query: 152 TALDVSFFRERGIHDLPSTAVSLRFQLQWFKQAYSSFCASST------------------ 211
           TAL+ SF  ERGIH  P T VSL  QL  FK++  + C S +                  
Sbjct: 152 TALERSFLEERGIH-FPYTNVSLAVQLSSFKESLPNLCVSPSDCRDMIENSLILMGEIGG 211

Query: 212 ---------------------LSVETL---------------MVPANIPIGCLPVYLQLY 271
                                L +ET+               +VP   P+GC   YL LY
Sbjct: 212 NDYNYAFFVGKNIEEIKELVPLVIETISSAITELIGMGGKTFLVPGEFPLGCSVAYLSLY 271

Query: 272 ETSNQDQYDPQNSCLKWLNRFSEYHNEQLHEELKRIRALYPHVRLIYVDSFNAALRVYNA 331
           +TSN ++YDP   CLKWLN+FSEYH+EQL  EL R++ LYPHV +IY D +N  LR+   
Sbjct: 272 QTSNIEEYDPLTGCLKWLNKFSEYHDEQLQAELNRLQKLYPHVNIIYADYYNTLLRLAQE 331

Query: 332 PKDFGFIDSREVCCVDGNGSYYFPL--PCGLPSTIVCDDPSKYVSWDGLHLTEAAYKVIA 361
           P  FGFI      C    G + F L    G      CDDPSKYVSWDG+H+TEAAY+++A
Sbjct: 332 PAKFGFISRPLPACCALGGPFNFTLGRKRGTQVPECCDDPSKYVSWDGVHMTEAAYRLMA 382

BLAST of Spg012000 vs. TAIR 10
Match: AT1G28610.2 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 249.2 bits (635), Expect = 5.1e-66
Identity = 152/404 (37.62%), Postives = 195/404 (48.27%), Query Frame = 0

Query: 7   SSSSSILQLFIPLAFVIFVPCVFGC--YTSIFNFGDSLSDTGNLYYTCSSPDPSQVCFFP 66
           +S  S++  F+   FV  V     C    SI +FGDS++DTGNL             F P
Sbjct: 2   ASLDSLVSFFLSTLFVTIVSSQTQCRNLESIISFGDSITDTGNLVGLSDRNHLPVTAFLP 61

Query: 67  YGETFFHRPTGRFSDGRVIPDFFGLISSPIPRFLCFVLFPEIWVLSDFGVFAAISLRLPL 126
           YGETFFH PTGR  +GR+I DF                      +++F       L LP 
Sbjct: 62  YGETFFHHPTGRSCNGRIIIDF----------------------IAEF-------LGLPH 121

Query: 127 LKPYRSGGEGISAEDFGNGLNFAVGGATALDVSFFRERGIHDLPSTAVSLRFQLQWFKQA 186
           + P+     G    +F  G+NFAV GATAL+ S   +RGI+  P + +SL  QL+ FK++
Sbjct: 122 VPPFYGSKNG----NFEKGVNFAVAGATALETSILEKRGIY-YPHSNISLGIQLKTFKES 181

Query: 187 YSSFCASST--------------------------------------------------- 246
             + C S T                                                   
Sbjct: 182 LPNLCGSPTDCRDMIGNAFIIMGEIGGNDFNFAFFVNKTSEVKELVPLVITKISSAIVEL 241

Query: 247 --LSVETLMVPANIPIGCLPVYLQLYETSNQDQYDPQNSCLKWLNRFSEYHNEQLHEELK 306
             +   T +VP N P+GC   YL LY+TSN+++YDP   CL WLN FSEY+NE+L  EL 
Sbjct: 242 VDMGGRTFLVPGNFPLGCSATYLTLYQTSNKEEYDPLTGCLTWLNDFSEYYNEKLQAELN 301

Query: 307 RIRALYPHVRLIYVDSFNAALRVYNAPKDFGFIDSREVCCVDGNGSYYFPL--PCGLPST 354
           R+  LYPHV +IY D FNA LR+Y  P  FGF+D     C    G Y F L   CG    
Sbjct: 302 RLSKLYPHVNIIYGDYFNALLRLYQEPSKFGFMDRPLPACCGLGGPYNFTLSKKCGSVGV 361

BLAST of Spg012000 vs. TAIR 10
Match: AT1G28600.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 247.7 bits (631), Expect = 1.5e-65
Identity = 154/405 (38.02%), Postives = 199/405 (49.14%), Query Frame = 0

Query: 7   SSSSSILQLFIPLAFVIFVPCVFGC--YTSIFNFGDSLSDTGNLYYTCSSPDPSQVCFFP 66
           S  S ++ LF  L FV  V     C  + SI +FGDS++DTGNL             F P
Sbjct: 3   SLDSLVIFLFSTL-FVTIVSSETPCPNFKSIISFGDSIADTGNLVGLSDRNQLPVTAFPP 62

Query: 67  YGETFFHRPTGRFSDGRVIPDFFGLISSPIPRFLCFVLFPEIWVLSDFGVFAAISLRLPL 126
           YGETFFH PTGR  DGR+I DF                      +++F       + LP 
Sbjct: 63  YGETFFHHPTGRSCDGRIIMDF----------------------IAEF-------VGLPY 122

Query: 127 LKPYRSGGEGISAEDFGNGLNFAVGGATALDVSFFRERGIHDLPSTAVSLRFQLQWFKQA 186
           + PY     G    +F  G+NFAV GATAL  SF ++RGI   P T VSL  QL+ FK++
Sbjct: 123 VPPY----FGSKNRNFDKGVNFAVAGATALKSSFLKKRGIQ--PHTNVSLGVQLKSFKKS 182

Query: 187 YSSFCASST--------------------------------------------------- 246
             + C S +                                                   
Sbjct: 183 LPNLCGSPSDCRDMIGNALILMGEIGGNDYNFPFFNRKPVKEVEELVPFVIASISSTITE 242

Query: 247 ---LSVETLMVPANIPIGCLPVYLQLYETSNQDQYDPQNSCLKWLNRFSEYHNEQLHEEL 306
              +  +T +VP   PIGC  VYL LY+TSN+D+YDP   CLKWLN+F EYH+E+L  EL
Sbjct: 243 LIGMGGKTFLVPGEFPIGCSVVYLTLYKTSNKDEYDPSTGCLKWLNKFGEYHSEKLKVEL 302

Query: 307 KRIRALYPHVRLIYVDSFNAALRVYNAPKDFGFIDSR-EVCC-VDGNGSYYFPLPCGLPS 354
            R+R LYPHV +IY D +N+ LR++  P  FGF++     CC + G  ++ F   CG   
Sbjct: 303 NRLRKLYPHVNIIYADYYNSLLRIFKEPAKFGFMERPFPACCGIGGPYNFNFTRKCGSVG 362

BLAST of Spg012000 vs. TAIR 10
Match: AT1G28580.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 246.9 bits (629), Expect = 2.5e-65
Identity = 141/377 (37.40%), Postives = 186/377 (49.34%), Query Frame = 0

Query: 32  YTSIFNFGDSLSDTGNLYYTCSSPDPSQVCFFPYGETFFHRPTGRFSDGRVIPDFFGLIS 91
           + SI +FGDS++DTGNL       D   + F PYGE FFH PTGRFS+GR+I DF     
Sbjct: 35  FKSIISFGDSIADTGNLLGLSDPKDLPHMAFPPYGENFFHHPTGRFSNGRLIIDF----- 94

Query: 92  SPIPRFLCFVLFPEIWVLSDFGVFAAISLRLPLLKPYRSGGEGISAEDFGNGLNFAVGGA 151
                            +++F       L LPL+ P+     G    +F  G+NFAVGGA
Sbjct: 95  -----------------IAEF-------LGLPLVPPF----YGSHNANFEKGVNFAVGGA 154

Query: 152 TALDVSFFRERGIHDLPSTAVSLRFQLQWFKQAYSSFCASST------------------ 211
           TAL+ SF  +RGIH  P T VSL  QL  FK++  S C S +                  
Sbjct: 155 TALERSFLEDRGIH-FPYTNVSLGVQLNSFKESLPSICGSPSDCRDMIENALILMGEIGG 214

Query: 212 ------------------------------------LSVETLMVPANIPIGCLPVYLQLY 271
                                               +   T +VP   P+GC  +YL  +
Sbjct: 215 NDYNYAFFVDKGIEEIKELMPLVITTISSAITELIGMGGRTFLVPGEFPVGCSVLYLTSH 274

Query: 272 ETSNQDQYDPQNSCLKWLNRFSEYHNEQLHEELKRIRALYPHVRLIYVDSFNAALRVYNA 331
           +TSN ++YDP   CLKWLN+F E H EQL  EL R++ LYPHV +IY D +NA   +Y  
Sbjct: 275 QTSNMEEYDPLTGCLKWLNKFGENHGEQLRAELNRLQKLYPHVNIIYADYYNALFHLYQE 334

Query: 332 PKDFGFIDSREVCCVDGNGSYYFPL--PCGLPSTIVCDDPSKYVSWDGLHLTEAAYKVIA 353
           P  FGF++     C    G Y + +   CG      CDDPSKYV+WDG+H+TEAAY+++A
Sbjct: 335 PAKFGFMNRPLSACCGAGGPYNYTVGRKCGTDIVESCDDPSKYVAWDGVHMTEAAYRLMA 377

BLAST of Spg012000 vs. TAIR 10
Match: AT2G27360.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 244.2 bits (622), Expect = 1.6e-64
Identity = 149/401 (37.16%), Postives = 196/401 (48.88%), Query Frame = 0

Query: 12  ILQLFIPLAFVIFVPCVFGC--YTSIFNFGDSLSDTGNLYYTCSSPDPSQVCFFPYGETF 71
           +L  FI    +  V     C  + SI +FGDS++DTGNL    S  D  +  F PYGETF
Sbjct: 9   LLSFFISTFLITVVTSQTRCRNFKSIISFGDSITDTGNLLGLSSPNDLPESAFPPYGETF 68

Query: 72  FHRPTGRFSDGRVIPDFFGLISSPIPRFLCFVLFPEIWVLSDFGVFAAISLRLPLLKPYR 131
           FH P+GRFSDGR+I DF                      +++F       L +P + P+ 
Sbjct: 69  FHHPSGRFSDGRLIIDF----------------------IAEF-------LGIPHVPPFY 128

Query: 132 SGGEGISAEDFGNGLNFAVGGATALDVSFFRERGIHDLPSTAVSLRFQLQWFKQAYSSFC 191
               G    +F  G+NFAVGGATAL+ S   E+G H   S  +SL  QL+ FK++    C
Sbjct: 129 GSKNG----NFEKGVNFAVGGATALECSVLEEKGTHCSQSN-ISLGNQLKSFKESLPYLC 188

Query: 192 ASST-------------------------------------------------------L 251
            SS+                                                       +
Sbjct: 189 GSSSPDCRDMIENAFILIGEIGGNDYNFPLFDRKNIEEVKELVPLVITTISSAISELVDM 248

Query: 252 SVETLMVPANIPIGCLPVYLQLYETSNQDQYDPQNSCLKWLNRFSEYHNEQLHEELKRIR 311
              T +VP N P+GC   YL LYET N+++Y+P   CL WLN FS YHNEQL  ELKR+R
Sbjct: 249 GARTFLVPGNFPLGCSVAYLTLYETPNKEEYNPLTGCLTWLNDFSVYHNEQLQAELKRLR 308

Query: 312 ALYPHVRLIYVDSFNAALRVYNAPKDFGFID-SREVCC-VDGNGSYYFPLPCGLPSTIVC 354
            LYPHV +IY D +N  LR+   P  FG +D     CC + G  ++ F + CG      C
Sbjct: 309 NLYPHVNIIYGDYYNTLLRLMQEPSKFGLMDRPLPACCGLGGPYNFTFSIKCGSKGVEYC 368