Spg011737 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg011737
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Descriptionbifunctional epoxide hydrolase 2-like
Locationscaffold1: 3210541 .. 3212618 (-)
RNA-Seq ExpressionSpg011737
SyntenySpg011737
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GGCCGTTGAAGGAAGCAAGAGGATACCCACACGTAATCGATTCTTCCCTTACATCGCAGAATTGAATTCAATATAATGGAGATCATCGCAGCTTCACATTTTACTGAAGAAGCTCTCTCTCCATGTCCATGGATCAAATTCAGCACAACTTCATCCAAGTTCGAGGACTCAAGCTCCACGTAGCCGAGATCGGAACTGGTAAGTAATCGGTCATCTCGTCGCCATTTTCCCCCAATTTCTAGGTCACTACTCACAGCATTTGCTCTCAGGTTCCAATGTCGTCGTCTTTCTGCACGGTTTTCCGGAGATTTGGTACTCGTGGCGGCACCAGATGATCGCTCTGGCTAACGCCGGATTCCGAGCCATTGCTCCCGATTTCAGAGGATATGGACTCTCCGATGCGCCGGCGGAGCCTTCGAAGGTTGAATTCAGCGATCTCGTCAGCGATCTCCTTGGAATTCTCGACGCTCTCCACATCCCTAAGGTCCTATTCTCCGTCCGATTCGTTAATCATTCATATTTTTTTTTTTAACTATTTGACAAGGTATAGCAATTTTCACTTTAAACTTTCTTTCTTCTTTTTTTTTTTTTGGGTTAAATTACAAGTGTTGCAGCTTTACTTTTAATAAACTTTCATTTGAAAAATCGTCCATAAGAATATGTTGATGTGCATTTAATGAATGCAAAACATATGTCTAGTACCAAATTAATGAAAGATTTATTTATAATCTTTTTCAAAAAAGAAAAGAAAGATTTATTTATAATCTTTCATCTGGTGCATTAATAAGACTTAATTTTGCGAAATTAGTGAGTTTTGGAAGATTTTTACTTTACTTTCTTGTTAAATTTTAGGATGAATCTAGCAAAAATTGAACCTAAGGAAAAGTTTGGACACATTTTTTAGTTTATGGTTCAATCTAATGAAACTGAAAGTATGCAATGAAAATTTATTCAATTTAACGATTTTTTTTTCTTTTTTACGTTTAAAAAATATCTATAATACTCTGTTTAGGTGTTCCTTGTTGCGAAAGATTTTGGAGCAAGGCCAGCCTTCTGTTTTGCTATTAAACACCAAAGGAGGGTATTGGGAGTTGTGACATTGGGAGTGCCATTCATACCTCCTGGACCTCACAAGTTCCCTCAAGGCTTCTATATCTCAAGGTGGCAGGTATATATATGAACATACTCTACCAATTACCATTTCAACTTGTTTCTTTTATACTTAATCTAAGCGATTTCTTATCTAGGAACCTCGGCGAGCTGAAGCTGATTTTGGTCGTTTTGATGCAAAAACTATTATCAGGATTGTCTATATCCTCTTCTCCAAAAGTGAAATCCCTGTAGCTCAAGAAAACCAGGAGATAATGGACTTAGTAGACCCATCTTCCCCTCTTCCCCCTTGGTTAAGTGAGGAGGATCTAATAACTTATGGAGCTCTTTATGAGAAATCTGGATTCAGTACCGCGTTAAAAGTTCCTTATAGGTAATGCTCTTGAATCTAGATTATTATTGCTTTTTAACTATGCTAGTGAATTGGAGTCCTATTCCAACTTTACCTAGTTTCTCAACTTTATAATCCTCTCATATCAACCTGATCCTTAATGTTTAGAGTAATCGATTTTGACACAAATAATTTTGGTTGTATTTGATGCATTATAGGTCTTTAAATGAAGACTGGGGCATACAAGATCCAAAAGTTAAAGTTCCAACAATGCTTATAATGGGTGAAAAGGATTATACTCGCAAATTTCCTAACATTGATGAATACATTCAAAGTGGAAAAATGGAAGAGTATGTTCCCAATTTGGAGATAATCTATTTACCAGAGGGATCGCACTTTGTTCAAGAACAATGCCCTAAAAAAGTTAATGAGCTACTTCTTACTTTCCTTGCCAAGAATACTTGACTCTTGACAAAGGCAACTGTTGTGTGCAAATGTATGGAGGTCAAGTATCAATGTGTTGTTTGGAGAATTGGCCCCACTTTGTTTGGGTTGAATGTATAATTTTTTACTTTTTGAGAAAAATACAAGAGTATGTACTAGAATAATTAAAATGGTACTTTACGGTTCACCGATG

mRNA sequence

ATGTCCATGGATCAAATTCAGCACAACTTCATCCAAGTTCGAGGACTCAAGCTCCACGTAGCCGAGATCGGAACTGGTTCCAATGTCGTCGTCTTTCTGCACGGTTTTCCGGAGATTTGGTACTCGTGGCGGCACCAGATGATCGCTCTGGCTAACGCCGGATTCCGAGCCATTGCTCCCGATTTCAGAGGATATGGACTCTCCGATGCGCCGGCGGAGCCTTCGAAGGTTGAATTCAGCGATCTCGTCAGCGATCTCCTTGGAATTCTCGACGCTCTCCACATCCCTAAGGTGTTCCTTGTTGCGAAAGATTTTGGAGCAAGGCCAGCCTTCTGTTTTGCTATTAAACACCAAAGGAGGGTATTGGGAGTTGTGACATTGGGAGTGCCATTCATACCTCCTGGACCTCACAAGTTCCCTCAAGGCTTCTATATCTCAAGGTGGCAGGAACCTCGGCGAGCTGAAGCTGATTTTGGTCGTTTTGATGCAAAAACTATTATCAGGATTGTCTATATCCTCTTCTCCAAAAGTGAAATCCCTGTAGCTCAAGAAAACCAGGAGATAATGGACTTAGTAGACCCATCTTCCCCTCTTCCCCCTTGGTTAAGTGAGGAGGATCTAATAACTTATGGAGCTCTTTATGAGAAATCTGGATTCAGTACCGCGTTAAAAGTTCCTTATAGGTCTTTAAATGAAGACTGGGGCATACAAGATCCAAAAGTTAAAGTTCCAACAATGCTTATAATGGGTGAAAAGGATTATACTCGCAAATTTCCTAACATTGATGAATACATTCAAAGTGGAAAAATGGAAGAGTATGTTCCCAATTTGGAGATAATCTATTTACCAGAGGGATCGCACTTTGTTCAAGAACAATGCCCTAAAAAAGTTAATGAGCTACTTCTTACTTTCCTTGCCAAGAATACTTGA

Coding sequence (CDS)

ATGTCCATGGATCAAATTCAGCACAACTTCATCCAAGTTCGAGGACTCAAGCTCCACGTAGCCGAGATCGGAACTGGTTCCAATGTCGTCGTCTTTCTGCACGGTTTTCCGGAGATTTGGTACTCGTGGCGGCACCAGATGATCGCTCTGGCTAACGCCGGATTCCGAGCCATTGCTCCCGATTTCAGAGGATATGGACTCTCCGATGCGCCGGCGGAGCCTTCGAAGGTTGAATTCAGCGATCTCGTCAGCGATCTCCTTGGAATTCTCGACGCTCTCCACATCCCTAAGGTGTTCCTTGTTGCGAAAGATTTTGGAGCAAGGCCAGCCTTCTGTTTTGCTATTAAACACCAAAGGAGGGTATTGGGAGTTGTGACATTGGGAGTGCCATTCATACCTCCTGGACCTCACAAGTTCCCTCAAGGCTTCTATATCTCAAGGTGGCAGGAACCTCGGCGAGCTGAAGCTGATTTTGGTCGTTTTGATGCAAAAACTATTATCAGGATTGTCTATATCCTCTTCTCCAAAAGTGAAATCCCTGTAGCTCAAGAAAACCAGGAGATAATGGACTTAGTAGACCCATCTTCCCCTCTTCCCCCTTGGTTAAGTGAGGAGGATCTAATAACTTATGGAGCTCTTTATGAGAAATCTGGATTCAGTACCGCGTTAAAAGTTCCTTATAGGTCTTTAAATGAAGACTGGGGCATACAAGATCCAAAAGTTAAAGTTCCAACAATGCTTATAATGGGTGAAAAGGATTATACTCGCAAATTTCCTAACATTGATGAATACATTCAAAGTGGAAAAATGGAAGAGTATGTTCCCAATTTGGAGATAATCTATTTACCAGAGGGATCGCACTTTGTTCAAGAACAATGCCCTAAAAAAGTTAATGAGCTACTTCTTACTTTCCTTGCCAAGAATACTTGA

Protein sequence

MSMDQIQHNFIQVRGLKLHVAEIGTGSNVVVFLHGFPEIWYSWRHQMIALANAGFRAIAPDFRGYGLSDAPAEPSKVEFSDLVSDLLGILDALHIPKVFLVAKDFGARPAFCFAIKHQRRVLGVVTLGVPFIPPGPHKFPQGFYISRWQEPRRAEADFGRFDAKTIIRIVYILFSKSEIPVAQENQEIMDLVDPSSPLPPWLSEEDLITYGALYEKSGFSTALKVPYRSLNEDWGIQDPKVKVPTMLIMGEKDYTRKFPNIDEYIQSGKMEEYVPNLEIIYLPEGSHFVQEQCPKKVNELLLTFLAKNT
Homology
BLAST of Spg011737 vs. NCBI nr
Match: XP_022950359.1 (uncharacterized protein LOC111453471 [Cucurbita moschata])

HSP 1 Score: 515.0 bits (1325), Expect = 4.5e-142
Identity = 239/311 (76.85%), Postives = 277/311 (89.07%), Query Frame = 0

Query: 3   MDQIQHNFIQVRGLKLHVAEIGTGSNVVVFLHGFPEIWYSWRHQMIALANAGFRAIAPDF 62
           MDQIQHNFI+V GLKLHVAEIGTGSNVVVFLHGFPEIWYSWR+QMIA+ANAGFRAIAPD+
Sbjct: 1   MDQIQHNFIEVAGLKLHVAEIGTGSNVVVFLHGFPEIWYSWRYQMIAIANAGFRAIAPDY 60

Query: 63  RGYGLSDAPAEPSKVEFSDLVSDLLGILDALHIPKVFLVAKDFGARPAFCFAIKHQRRVL 122
           RGYGLSD+PAEPSK  FSDL+SDLLGILDAL+IPKV +V KDFGA PA+ FA+KH  R L
Sbjct: 61  RGYGLSDSPAEPSKASFSDLISDLLGILDALNIPKVIIVGKDFGAWPAYYFALKHPERAL 120

Query: 123 GVVTLGVPFIPPGPHK-----FPQGFYISRWQEPRRAEADFGRFDAKTIIRIVYILFSKS 182
           GVVTLGVPF+PPG  +      P+G Y  RW+EP RAEADFGRFDAKT++R VYILFSKS
Sbjct: 121 GVVTLGVPFMPPGSVRSTQSHIPEGVYTLRWREPGRAEADFGRFDAKTVVRNVYILFSKS 180

Query: 183 EIPVAQENQEIMDLVDPSSPLPPWLSEEDLITYGALYEKSGFSTALKVPYRSLNEDWGIQ 242
           E+P+AQENQEIMDLVDPS+PLPPW +EEDL TYGALYEKSGF TALKVPYRS NEDWG++
Sbjct: 181 EMPIAQENQEIMDLVDPSTPLPPWFTEEDLATYGALYEKSGFDTALKVPYRSFNEDWGVK 240

Query: 243 DPKVKVPTMLIMGEKDYTRKFPNIDEYIQSGKMEEYVPNLEIIYLPEGSHFVQEQCPKKV 302
           DPKV++P + I+GEKDY  KFP+I+EY++SG+++E+VP LEIIYLPEGSHFVQEQ P++V
Sbjct: 241 DPKVEIPALFIVGEKDYVLKFPDIEEYVRSGRVKEFVPKLEIIYLPEGSHFVQEQSPEEV 300

Query: 303 NELLLTFLAKN 309
           N+LLLTFLA +
Sbjct: 301 NQLLLTFLANH 311

BLAST of Spg011737 vs. NCBI nr
Match: XP_023544288.1 (uncharacterized protein LOC111803907 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 514.6 bits (1324), Expect = 5.9e-142
Identity = 240/311 (77.17%), Postives = 276/311 (88.75%), Query Frame = 0

Query: 3   MDQIQHNFIQVRGLKLHVAEIGTGSNVVVFLHGFPEIWYSWRHQMIALANAGFRAIAPDF 62
           MDQIQHNFI+V GLKLHVAEIGTGSNVVVFLHGFPEIWYSWR+QMIALANAGFRAIAPD+
Sbjct: 1   MDQIQHNFIEVAGLKLHVAEIGTGSNVVVFLHGFPEIWYSWRYQMIALANAGFRAIAPDY 60

Query: 63  RGYGLSDAPAEPSKVEFSDLVSDLLGILDALHIPKVFLVAKDFGARPAFCFAIKHQRRVL 122
           RGYGLSD+PAEPSK  FSDL+SDLLGILDAL+IPKV +V KDFGA PA+ FA+KH  R L
Sbjct: 61  RGYGLSDSPAEPSKASFSDLISDLLGILDALNIPKVIIVGKDFGAWPAYYFALKHPERAL 120

Query: 123 GVVTLGVPFIPPGPHK-----FPQGFYISRWQEPRRAEADFGRFDAKTIIRIVYILFSKS 182
           GVVTLGVPF+PPG  +      P+G Y  RW+EP RAEADFGRFDAKT++R VYILFSKS
Sbjct: 121 GVVTLGVPFMPPGSVRSTQSHIPEGVYTLRWREPGRAEADFGRFDAKTVVRNVYILFSKS 180

Query: 183 EIPVAQENQEIMDLVDPSSPLPPWLSEEDLITYGALYEKSGFSTALKVPYRSLNEDWGIQ 242
           E+P+AQENQEIMDLVDPS+PLPPW +EEDL TYGALYEKSGF TALKVPYRS NEDWGI+
Sbjct: 181 EMPIAQENQEIMDLVDPSTPLPPWFTEEDLATYGALYEKSGFDTALKVPYRSFNEDWGIR 240

Query: 243 DPKVKVPTMLIMGEKDYTRKFPNIDEYIQSGKMEEYVPNLEIIYLPEGSHFVQEQCPKKV 302
           DPKV++P + I+GEKDY  KFP I+EY++SG+++E+VP LEIIYLPEGSHFVQEQ P++V
Sbjct: 241 DPKVEIPALFIVGEKDYVLKFPGIEEYVRSGRVKEFVPKLEIIYLPEGSHFVQEQSPEEV 300

Query: 303 NELLLTFLAKN 309
           N+LLLTFL+ +
Sbjct: 301 NQLLLTFLSNH 311

BLAST of Spg011737 vs. NCBI nr
Match: XP_022142308.1 (bifunctional epoxide hydrolase 2-like isoform X2 [Momordica charantia])

HSP 1 Score: 514.6 bits (1324), Expect = 5.9e-142
Identity = 242/315 (76.83%), Postives = 275/315 (87.30%), Query Frame = 0

Query: 3   MDQIQHNFIQVRGLKLHVAEIGTGSNVVVFLHGFPEIWYSWRHQMIALANAGFRAIAPDF 62
           M+QIQH FIQVRGLKLHVAEIGTGSNVVVFLHGFPEIWYSWR+QMIALANAGFRAIAPD+
Sbjct: 1   MEQIQHKFIQVRGLKLHVAEIGTGSNVVVFLHGFPEIWYSWRYQMIALANAGFRAIAPDY 60

Query: 63  RGYGLSDAPAEPSKVEFSDLVSDLLGILDALHIPKVFLVAKDFGARPAFCFAIKHQRRVL 122
           RGYGLSDAP EPSK  FSDL+SDL+GILDAL+IPKVF++ KDFGA PA+ FA+KH  R L
Sbjct: 61  RGYGLSDAPPEPSKATFSDLISDLVGILDALNIPKVFVIGKDFGAWPAYYFALKHPDRAL 120

Query: 123 GVVTLGVPFIPP--------GPHKFPQGFYISRWQEPRRAEADFGRFDAKTIIRIVYILF 182
           GVVTLGVPF+PP          H  P+G Y  RW+EP RAEADFGRFDAKT++R VYILF
Sbjct: 121 GVVTLGVPFLPPESVNNSLKPQHHIPEGVYTLRWREPGRAEADFGRFDAKTVVRNVYILF 180

Query: 183 SKSEIPVAQENQEIMDLVDPSSPLPPWLSEEDLITYGALYEKSGFSTALKVPYRSLNEDW 242
           SKSEIP+AQENQE+MDLVDPS+PLPPW +EEDL TYGALYEKSGF TALKVPYRS NEDW
Sbjct: 181 SKSEIPIAQENQEVMDLVDPSTPLPPWFTEEDLATYGALYEKSGFETALKVPYRSFNEDW 240

Query: 243 GIQDPKVKVPTMLIMGEKDYTRKFPNIDEYIQSGKMEEYVPNLEIIYLPEGSHFVQEQCP 302
           GI+DPKV++P + IMGEKDY  KFP I+EYI+S ++++YVP LEIIYLPEGSHFVQEQ P
Sbjct: 241 GIKDPKVEIPALFIMGEKDYVFKFPEIEEYIRSERVKDYVPKLEIIYLPEGSHFVQEQSP 300

Query: 303 KKVNELLLTFLAKNT 310
           ++VN+LLLTFLA +T
Sbjct: 301 EEVNQLLLTFLANHT 315

BLAST of Spg011737 vs. NCBI nr
Match: XP_022978890.1 (uncharacterized protein LOC111478707 [Cucurbita maxima])

HSP 1 Score: 512.7 bits (1319), Expect = 2.2e-141
Identity = 240/311 (77.17%), Postives = 275/311 (88.42%), Query Frame = 0

Query: 3   MDQIQHNFIQVRGLKLHVAEIGTGSNVVVFLHGFPEIWYSWRHQMIALANAGFRAIAPDF 62
           MDQIQHNF++V GLKLHVAEIGTGSNVVVFLHGFPEIWYSWR QMIALANAGFRAIAPD+
Sbjct: 1   MDQIQHNFVEVGGLKLHVAEIGTGSNVVVFLHGFPEIWYSWRFQMIALANAGFRAIAPDY 60

Query: 63  RGYGLSDAPAEPSKVEFSDLVSDLLGILDALHIPKVFLVAKDFGARPAFCFAIKHQRRVL 122
           RGYGLSD+PAEPSK  FSDL+SDLLGIL+AL+IPKV +V KDFGA PA+ FA+KH  R L
Sbjct: 61  RGYGLSDSPAEPSKASFSDLISDLLGILNALNIPKVIIVGKDFGAWPAYYFALKHPERAL 120

Query: 123 GVVTLGVPFIPPGPHK-----FPQGFYISRWQEPRRAEADFGRFDAKTIIRIVYILFSKS 182
           GVVTLGVPF+PPG  +      P+G Y  RW+EP RAEADFGRFDAKT++R VYILFSKS
Sbjct: 121 GVVTLGVPFMPPGSVRSTQSHIPEGVYTLRWREPGRAEADFGRFDAKTVVRNVYILFSKS 180

Query: 183 EIPVAQENQEIMDLVDPSSPLPPWLSEEDLITYGALYEKSGFSTALKVPYRSLNEDWGIQ 242
           E+P+AQENQEIMDLVDPS+PLPPW +EEDL TYGALYEKSGF TALKVPYRS NEDWGI+
Sbjct: 181 EMPIAQENQEIMDLVDPSTPLPPWFTEEDLATYGALYEKSGFDTALKVPYRSFNEDWGIE 240

Query: 243 DPKVKVPTMLIMGEKDYTRKFPNIDEYIQSGKMEEYVPNLEIIYLPEGSHFVQEQCPKKV 302
           DPKV++P + I+GEKDY  KFP I+EYI+SG+++E+VP LEIIYLPEGSHFVQEQ P++V
Sbjct: 241 DPKVEIPALFIVGEKDYVLKFPGIEEYIRSGRVKEFVPKLEIIYLPEGSHFVQEQSPEEV 300

Query: 303 NELLLTFLAKN 309
           N+LLLTFLA +
Sbjct: 301 NQLLLTFLANH 311

BLAST of Spg011737 vs. NCBI nr
Match: XP_008439884.2 (PREDICTED: bifunctional epoxide hydrolase 2-like [Cucumis melo])

HSP 1 Score: 507.3 bits (1305), Expect = 9.4e-140
Identity = 236/310 (76.13%), Postives = 272/310 (87.74%), Query Frame = 0

Query: 3   MDQIQHNFIQVRGLKLHVAEIGTGSNVVVFLHGFPEIWYSWRHQMIALANAGFRAIAPDF 62
           MD+I HNFIQV  LKLHVAEIGTGSNVVVFLHGFPEIWYSWR+QMIALA+AGFR +APD+
Sbjct: 1   MDRIHHNFIQVGALKLHVAEIGTGSNVVVFLHGFPEIWYSWRYQMIALADAGFRVLAPDY 60

Query: 63  RGYGLSDAPAEPSKVEFSDLVSDLLGILDALHIPKVFLVAKDFGARPAFCFAIKHQRRVL 122
           RGYGLSD+PAEPSK  FSDL+SDLLGILDAL+IPKVF+VAKDFGA PA+ FA+KH  R L
Sbjct: 61  RGYGLSDSPAEPSKASFSDLISDLLGILDALNIPKVFVVAKDFGAWPAYYFALKHPERAL 120

Query: 123 GVVTLGVPFIPPGPHK-----FPQGFYISRWQEPRRAEADFGRFDAKTIIRIVYILFSKS 182
           G+VTLGVPF+PP   K      P+G Y  RW+EP RAEADFGRFDAKT++R VYILFSKS
Sbjct: 121 GIVTLGVPFLPPESLKHSQSNIPEGVYTLRWREPGRAEADFGRFDAKTVVRNVYILFSKS 180

Query: 183 EIPVAQENQEIMDLVDPSSPLPPWLSEEDLITYGALYEKSGFSTALKVPYRSLNEDWGIQ 242
           EIP AQENQE+MDLVDPS+PLPPW +EEDL TYGALYEKSGF TALKVPYRS NEDWGI+
Sbjct: 181 EIPTAQENQEVMDLVDPSTPLPPWFTEEDLATYGALYEKSGFDTALKVPYRSFNEDWGIK 240

Query: 243 DPKVKVPTMLIMGEKDYTRKFPNIDEYIQSGKMEEYVPNLEIIYLPEGSHFVQEQCPKKV 302
           DP+V++P + IMGEKDY  KFP +DEY++S +++++VPNLEI+YLPEGSHFVQEQ P++V
Sbjct: 241 DPRVEIPALFIMGEKDYVFKFPEVDEYVRSERVKDFVPNLEIVYLPEGSHFVQEQSPEEV 300

Query: 303 NELLLTFLAK 308
           N LLLTFLAK
Sbjct: 301 NHLLLTFLAK 310

BLAST of Spg011737 vs. ExPASy Swiss-Prot
Match: I6YGS0 (Epoxide hydrolase A OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX=83332 GN=ephA PE=1 SV=1)

HSP 1 Score: 145.6 bits (366), Expect = 9.4e-34
Identity = 98/316 (31.01%), Postives = 151/316 (47.78%), Query Frame = 0

Query: 11  IQVRGLKLHVAEIG-TGSNVVVFLHGFPEIWYSWRHQMIALANAGFRAIAPDFRGYGLSD 70
           +   G++L V E G  G+ VV+  HGFPE+ YSWRHQ+ ALA+AG+  +APD RGYG S 
Sbjct: 9   VDTNGVRLRVVEAGEPGAPVVILAHGFPELAYSWRHQIPALADAGYHVLAPDQRGYGGSS 68

Query: 71  APAEPSKVEFSDLVSDLLGILDALHIPKVFLVAKDFGARPAFCFAIKHQRRVLGVVTLGV 130
            P      +   L +DL+G+LD +   +   V  D+GA   +   + H  RV  V  L V
Sbjct: 69  RPEAIEAYDIHRLTADLVGLLDDVGAERAVWVGHDWGAVVVWNAPLLHADRVAAVAALSV 128

Query: 131 PFIP----PGPHKF-----PQGFYISRWQEPRRAEADFGRFDAKTIIRIVYILFSKSEIP 190
           P +P    P    F        FYI  +QEP  A+A+     A+T+ R++  L    +  
Sbjct: 129 PALPRAQVPPTQAFRSRFGENFFYILYFQEPGIADAELNGDPARTMRRMIGGLRPPGDQS 188

Query: 191 VAQE-----NQEIMDLVDPSSPLPPWLSEEDLITYGALYEKSGFSTALKVPYRSLNEDW- 250
            A           +D +   + LP W+S+E+L  Y   + ++GF+  L   YR+ + +W 
Sbjct: 189 AAMRMLAPGPDGFIDRLPEPAGLPAWISQEELDHYIGEFTRTGFTGGLNW-YRNFDRNWE 248

Query: 251 ---GIQDPKVKVPTMLIMGEKDYTRKFPNIDEYIQ--SGKMEEYVPNLEIIYLPEGSHFV 306
               +    + VP++ I G  D    F   D   +  SG   E       + +    H++
Sbjct: 249 TTADLAGKTISVPSLFIAGTADPVLTFTRTDRAAEVISGPYRE-------VLIDGAGHWL 308

BLAST of Spg011737 vs. ExPASy Swiss-Prot
Match: P34913 (Bifunctional epoxide hydrolase 2 OS=Homo sapiens OX=9606 GN=EPHX2 PE=1 SV=2)

HSP 1 Score: 143.7 bits (361), Expect = 3.6e-33
Identity = 94/318 (29.56%), Postives = 161/318 (50.63%), Query Frame = 0

Query: 6   IQHNFIQVR-GLKLHVAEIGTGSNVVVFLHGFPEIWYSWRHQMIALANAGFRAIAPDFRG 65
           + H ++ V+  ++LH  E+G+G   V   HGFPE WYSWR+Q+ ALA AG+R +A D +G
Sbjct: 237 MSHGYVTVKPRVRLHFVELGSGP-AVCLCHGFPESWYSWRYQIPALAQAGYRVLAMDMKG 296

Query: 66  YGLSDAPAEPSKVEFSDLVSDLLGILDALHIPKVFLVAKDFGARPAFCFAIKHQRRVLGV 125
           YG S AP E  +     L  +++  LD L + +   +  D+G    +  A+ +  RV  V
Sbjct: 297 YGESSAPPEIEEYCMEVLCKEMVTFLDKLGLSQAVFIGHDWGGMLVWYMALFYPERVRAV 356

Query: 126 VTLGVPFIPPGPHKFPQGF--------YISRWQEPRRAEADFGRFDAKTIIRIVYILFSK 185
            +L  PFIP  P+  P           Y   +QEP  AEA+      + + R    LF  
Sbjct: 357 ASLNTPFIPANPNMSPLESIKANPVFDYQLYFQEPGVAEAEL----EQNLSRTFKSLFRA 416

Query: 186 SEIPVAQENQEI----MDLVDPSSP-LPPWLSEEDLITYGALYEKSGFSTALKVPYRSLN 245
           S+  V   ++      + +  P  P L   ++EE++  Y   ++KSGF   L   YR++ 
Sbjct: 417 SDESVLSMHKVCEAGGLFVNSPEEPSLSRMVTEEEIQFYVQQFKKSGFRGPLNW-YRNME 476

Query: 246 EDW----GIQDPKVKVPTMLIMGEKDYTRKFPNIDEYIQSGKMEEYVPNLEIIYLPEGSH 305
            +W         K+ +P +++  EKD+    P + ++     ME+++P+L+  ++ +  H
Sbjct: 477 RNWKWACKSLGRKILIPALMVTAEKDFV-LVPQMSQH-----MEDWIPHLKRGHIEDCGH 536

BLAST of Spg011737 vs. ExPASy Swiss-Prot
Match: P34914 (Bifunctional epoxide hydrolase 2 OS=Mus musculus OX=10090 GN=Ephx2 PE=1 SV=2)

HSP 1 Score: 142.5 bits (358), Expect = 7.9e-33
Identity = 96/318 (30.19%), Postives = 161/318 (50.63%), Query Frame = 0

Query: 4   DQIQHNFIQVR-GLKLHVAEIGTGSNVVVFLHGFPEIWYSWRHQMIALANAGFRAIAPDF 63
           + + H ++ V+ G++LH  E+G+G   +   HGFPE W+SWR+Q+ ALA AGFR +A D 
Sbjct: 233 NDVSHGYVTVKPGIRLHFVEMGSGP-ALCLCHGFPESWFSWRYQIPALAQAGFRVLAIDM 292

Query: 64  RGYGLSDAPAEPSKVEFSDLVSDLLGILDALHIPKVFLVAKDFGARPAFCFAIKHQRRVL 123
           +GYG S +P E  +     L  +++  LD L IP+   +  D+     +  A+ +  RV 
Sbjct: 293 KGYGDSSSPPEIEEYAMELLCKEMVTFLDKLGIPQAVFIGHDWAGVMVWNMALFYPERVR 352

Query: 124 GVVTLGVPFIPPGP--------HKFPQGFYISRWQEPRRAEADFGRFDAKTIIRIVYILF 183
            V +L  PF+PP P           P   Y   +QEP  AEA+  +  ++T         
Sbjct: 353 AVASLNTPFMPPDPDVSPMKVIRSIPVFNYQLYFQEPGVAEAELEKNMSRTFKSFFRASD 412

Query: 184 SKSEIPVAQENQEIMDLVD-PSSP-LPPWLSEEDLITYGALYEKSGFSTALKVPYRSLNE 243
               I V +  +    LV+ P  P L    +EE++  Y   ++K+GF   L   YR+   
Sbjct: 413 ETGFIAVHKATEIGGILVNTPEDPNLSKITTEEEIEFYIQQFKKTGFRGPLNW-YRNTER 472

Query: 244 DW-----GIQDPKVKVPTMLIMGEKDYTRKFPNIDEYIQSGKMEEYVPNLEIIYLPEGSH 303
           +W     G+   K+ VP +++  EKD   + P +     S  ME+++P L+  ++ +  H
Sbjct: 473 NWKWSCKGL-GRKILVPALMVTAEKDIVLR-PEM-----SKNMEKWIPFLKRGHIEDCGH 532

Query: 304 FVQEQCPKKVNELLLTFL 306
           + Q + P +VN++L+ +L
Sbjct: 533 WTQIEKPTEVNQILIKWL 541

BLAST of Spg011737 vs. ExPASy Swiss-Prot
Match: P80299 (Bifunctional epoxide hydrolase 2 OS=Rattus norvegicus OX=10116 GN=Ephx2 PE=1 SV=1)

HSP 1 Score: 141.4 bits (355), Expect = 1.8e-32
Identity = 95/323 (29.41%), Postives = 156/323 (48.30%), Query Frame = 0

Query: 2   SMDQIQHNFIQVR-GLKLHVAEIGTGSNVVVFLHGFPEIWYSWRHQMIALANAGFRAIAP 61
           S + + H ++ V+ G++LH  E+G+G   +   HGFPE W+SWR+Q+ ALA AGFR +A 
Sbjct: 231 SPNDVSHGYVTVKPGIRLHFVEMGSGP-AICLCHGFPESWFSWRYQIPALAQAGFRVLAI 290

Query: 62  DFRGYGLSDAPAEPSKVEFSDLVSDLLGILDALHIPKVFLVAKDFGARPAFCFAIKHQRR 121
           D +GYG S +P E  +     L  +++  L+ L IP+   +  D+     +  A+ H  R
Sbjct: 291 DMKGYGDSSSPPEIEEYAMELLCEEMVTFLNKLGIPQAVFIGHDWAGVLVWNMALFHPER 350

Query: 122 VLGVVTLGVPFIPPGPHKFPQGF--------YISRWQEPRRAEADFGRFDAKTIIRIVYI 181
           V  V +L  P +PP P   P           Y   +QEP  AEA+      K + R    
Sbjct: 351 VRAVASLNTPLMPPNPEVSPMEVIRSIPVFNYQLYFQEPGVAEAEL----EKNMSRTFKS 410

Query: 182 LFSKSE------IPVAQENQEIMDLVDPSSPLPPWLSEEDLITYGALYEKSGFSTALKVP 241
            F  S+      +  A E   I+        +    +EE++  Y   ++KSGF   L   
Sbjct: 411 FFRTSDDMGLLTVNKATEMGGILVGTPEDPKVSKITTEEEIEYYIQQFKKSGFRGPLNW- 470

Query: 242 YRSLNEDW----GIQDPKVKVPTMLIMGEKDYTRKFPNIDEYIQSGKMEEYVPNLEIIYL 301
           YR+   +W         K+ VP +++  EKD   + P +     S  ME ++P L+  ++
Sbjct: 471 YRNTERNWKWSCKALGRKILVPALMVTAEKDIVLR-PEM-----SKNMENWIPFLKRGHI 530

Query: 302 PEGSHFVQEQCPKKVNELLLTFL 306
            +  H+ Q + P +VN++L+ +L
Sbjct: 531 EDCGHWTQIEKPAEVNQILIKWL 541

BLAST of Spg011737 vs. ExPASy Swiss-Prot
Match: Q6Q2C2 (Bifunctional epoxide hydrolase 2 OS=Sus scrofa OX=9823 GN=EPHX2 PE=2 SV=1)

HSP 1 Score: 137.1 bits (344), Expect = 3.3e-31
Identity = 94/318 (29.56%), Postives = 158/318 (49.69%), Query Frame = 0

Query: 6   IQHNFIQVR-GLKLHVAEIGTGSNVVVFLHGFPEIWYSWRHQMIALANAGFRAIAPDFRG 65
           + H ++ ++ G++LH  E+G+G   V   HGFPE W+SWR+Q+ ALA AGFR +A D +G
Sbjct: 237 LSHGYVLIKPGVRLHFVEMGSGP-AVCLCHGFPESWFSWRYQIPALAQAGFRVLAVDMKG 296

Query: 66  YGLSDAPAEPSKVEFSDLVSDLLGILDALHIPKVFLVAKDFGARPAFCFAIKHQRRVLGV 125
           YG S AP E  +     L  D++  L+ L + +   +  D+G    +  A+ +  RV  V
Sbjct: 297 YGESSAPPEIEEYSLEVLCKDMVTFLNKLGLSQAVFIGHDWGGVLVWNMALFYPERVRAV 356

Query: 126 VTLGVPFIPPGPHKFPQGF--------YISRWQEPRRAEADFGRFDAKTIIRIVYILFSK 185
            +L  PF+P  P+  P           Y   +QEP  AEA+      + + R     F  
Sbjct: 357 ASLNTPFMPSNPNVSPMEIIKANPVFDYQLYFQEPGVAEAEL----EQNLDRTFKNFFRA 416

Query: 186 SEIPVAQEN--QEIMDLV--DPSSP-LPPWLSEEDLITYGALYEKSGFSTALKVPYRSL- 245
            +      N  +E+  L    P  P L   ++EED+  Y   ++KSGF   L   YR++ 
Sbjct: 417 HDETFLTTNRVRELGGLFVGTPEEPSLSRLVTEEDIQFYVQQFKKSGFRGPLNW-YRNME 476

Query: 246 -NEDWGIQDP--KVKVPTMLIMGEKDYTRKFPNIDEYIQSGKMEEYVPNLEIIYLPEGSH 305
            N  WG +    K+ +P +++  E D     P + ++     ME ++P+L+  ++ +  H
Sbjct: 477 RNWQWGCKGSGRKILIPALMVTAENDLV-LHPKMSKH-----MENWIPHLKRGHIKDCGH 536

BLAST of Spg011737 vs. ExPASy TrEMBL
Match: A0A6J1GFK2 (uncharacterized protein LOC111453471 OS=Cucurbita moschata OX=3662 GN=LOC111453471 PE=4 SV=1)

HSP 1 Score: 515.0 bits (1325), Expect = 2.2e-142
Identity = 239/311 (76.85%), Postives = 277/311 (89.07%), Query Frame = 0

Query: 3   MDQIQHNFIQVRGLKLHVAEIGTGSNVVVFLHGFPEIWYSWRHQMIALANAGFRAIAPDF 62
           MDQIQHNFI+V GLKLHVAEIGTGSNVVVFLHGFPEIWYSWR+QMIA+ANAGFRAIAPD+
Sbjct: 1   MDQIQHNFIEVAGLKLHVAEIGTGSNVVVFLHGFPEIWYSWRYQMIAIANAGFRAIAPDY 60

Query: 63  RGYGLSDAPAEPSKVEFSDLVSDLLGILDALHIPKVFLVAKDFGARPAFCFAIKHQRRVL 122
           RGYGLSD+PAEPSK  FSDL+SDLLGILDAL+IPKV +V KDFGA PA+ FA+KH  R L
Sbjct: 61  RGYGLSDSPAEPSKASFSDLISDLLGILDALNIPKVIIVGKDFGAWPAYYFALKHPERAL 120

Query: 123 GVVTLGVPFIPPGPHK-----FPQGFYISRWQEPRRAEADFGRFDAKTIIRIVYILFSKS 182
           GVVTLGVPF+PPG  +      P+G Y  RW+EP RAEADFGRFDAKT++R VYILFSKS
Sbjct: 121 GVVTLGVPFMPPGSVRSTQSHIPEGVYTLRWREPGRAEADFGRFDAKTVVRNVYILFSKS 180

Query: 183 EIPVAQENQEIMDLVDPSSPLPPWLSEEDLITYGALYEKSGFSTALKVPYRSLNEDWGIQ 242
           E+P+AQENQEIMDLVDPS+PLPPW +EEDL TYGALYEKSGF TALKVPYRS NEDWG++
Sbjct: 181 EMPIAQENQEIMDLVDPSTPLPPWFTEEDLATYGALYEKSGFDTALKVPYRSFNEDWGVK 240

Query: 243 DPKVKVPTMLIMGEKDYTRKFPNIDEYIQSGKMEEYVPNLEIIYLPEGSHFVQEQCPKKV 302
           DPKV++P + I+GEKDY  KFP+I+EY++SG+++E+VP LEIIYLPEGSHFVQEQ P++V
Sbjct: 241 DPKVEIPALFIVGEKDYVLKFPDIEEYVRSGRVKEFVPKLEIIYLPEGSHFVQEQSPEEV 300

Query: 303 NELLLTFLAKN 309
           N+LLLTFLA +
Sbjct: 301 NQLLLTFLANH 311

BLAST of Spg011737 vs. ExPASy TrEMBL
Match: A0A6J1CMZ3 (bifunctional epoxide hydrolase 2-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111012457 PE=4 SV=1)

HSP 1 Score: 514.6 bits (1324), Expect = 2.8e-142
Identity = 242/315 (76.83%), Postives = 275/315 (87.30%), Query Frame = 0

Query: 3   MDQIQHNFIQVRGLKLHVAEIGTGSNVVVFLHGFPEIWYSWRHQMIALANAGFRAIAPDF 62
           M+QIQH FIQVRGLKLHVAEIGTGSNVVVFLHGFPEIWYSWR+QMIALANAGFRAIAPD+
Sbjct: 1   MEQIQHKFIQVRGLKLHVAEIGTGSNVVVFLHGFPEIWYSWRYQMIALANAGFRAIAPDY 60

Query: 63  RGYGLSDAPAEPSKVEFSDLVSDLLGILDALHIPKVFLVAKDFGARPAFCFAIKHQRRVL 122
           RGYGLSDAP EPSK  FSDL+SDL+GILDAL+IPKVF++ KDFGA PA+ FA+KH  R L
Sbjct: 61  RGYGLSDAPPEPSKATFSDLISDLVGILDALNIPKVFVIGKDFGAWPAYYFALKHPDRAL 120

Query: 123 GVVTLGVPFIPP--------GPHKFPQGFYISRWQEPRRAEADFGRFDAKTIIRIVYILF 182
           GVVTLGVPF+PP          H  P+G Y  RW+EP RAEADFGRFDAKT++R VYILF
Sbjct: 121 GVVTLGVPFLPPESVNNSLKPQHHIPEGVYTLRWREPGRAEADFGRFDAKTVVRNVYILF 180

Query: 183 SKSEIPVAQENQEIMDLVDPSSPLPPWLSEEDLITYGALYEKSGFSTALKVPYRSLNEDW 242
           SKSEIP+AQENQE+MDLVDPS+PLPPW +EEDL TYGALYEKSGF TALKVPYRS NEDW
Sbjct: 181 SKSEIPIAQENQEVMDLVDPSTPLPPWFTEEDLATYGALYEKSGFETALKVPYRSFNEDW 240

Query: 243 GIQDPKVKVPTMLIMGEKDYTRKFPNIDEYIQSGKMEEYVPNLEIIYLPEGSHFVQEQCP 302
           GI+DPKV++P + IMGEKDY  KFP I+EYI+S ++++YVP LEIIYLPEGSHFVQEQ P
Sbjct: 241 GIKDPKVEIPALFIMGEKDYVFKFPEIEEYIRSERVKDYVPKLEIIYLPEGSHFVQEQSP 300

Query: 303 KKVNELLLTFLAKNT 310
           ++VN+LLLTFLA +T
Sbjct: 301 EEVNQLLLTFLANHT 315

BLAST of Spg011737 vs. ExPASy TrEMBL
Match: A0A6J1IRI3 (uncharacterized protein LOC111478707 OS=Cucurbita maxima OX=3661 GN=LOC111478707 PE=4 SV=1)

HSP 1 Score: 512.7 bits (1319), Expect = 1.1e-141
Identity = 240/311 (77.17%), Postives = 275/311 (88.42%), Query Frame = 0

Query: 3   MDQIQHNFIQVRGLKLHVAEIGTGSNVVVFLHGFPEIWYSWRHQMIALANAGFRAIAPDF 62
           MDQIQHNF++V GLKLHVAEIGTGSNVVVFLHGFPEIWYSWR QMIALANAGFRAIAPD+
Sbjct: 1   MDQIQHNFVEVGGLKLHVAEIGTGSNVVVFLHGFPEIWYSWRFQMIALANAGFRAIAPDY 60

Query: 63  RGYGLSDAPAEPSKVEFSDLVSDLLGILDALHIPKVFLVAKDFGARPAFCFAIKHQRRVL 122
           RGYGLSD+PAEPSK  FSDL+SDLLGIL+AL+IPKV +V KDFGA PA+ FA+KH  R L
Sbjct: 61  RGYGLSDSPAEPSKASFSDLISDLLGILNALNIPKVIIVGKDFGAWPAYYFALKHPERAL 120

Query: 123 GVVTLGVPFIPPGPHK-----FPQGFYISRWQEPRRAEADFGRFDAKTIIRIVYILFSKS 182
           GVVTLGVPF+PPG  +      P+G Y  RW+EP RAEADFGRFDAKT++R VYILFSKS
Sbjct: 121 GVVTLGVPFMPPGSVRSTQSHIPEGVYTLRWREPGRAEADFGRFDAKTVVRNVYILFSKS 180

Query: 183 EIPVAQENQEIMDLVDPSSPLPPWLSEEDLITYGALYEKSGFSTALKVPYRSLNEDWGIQ 242
           E+P+AQENQEIMDLVDPS+PLPPW +EEDL TYGALYEKSGF TALKVPYRS NEDWGI+
Sbjct: 181 EMPIAQENQEIMDLVDPSTPLPPWFTEEDLATYGALYEKSGFDTALKVPYRSFNEDWGIE 240

Query: 243 DPKVKVPTMLIMGEKDYTRKFPNIDEYIQSGKMEEYVPNLEIIYLPEGSHFVQEQCPKKV 302
           DPKV++P + I+GEKDY  KFP I+EYI+SG+++E+VP LEIIYLPEGSHFVQEQ P++V
Sbjct: 241 DPKVEIPALFIVGEKDYVLKFPGIEEYIRSGRVKEFVPKLEIIYLPEGSHFVQEQSPEEV 300

Query: 303 NELLLTFLAKN 309
           N+LLLTFLA +
Sbjct: 301 NQLLLTFLANH 311

BLAST of Spg011737 vs. ExPASy TrEMBL
Match: A0A1S3AZS9 (bifunctional epoxide hydrolase 2-like OS=Cucumis melo OX=3656 GN=LOC103484538 PE=4 SV=1)

HSP 1 Score: 507.3 bits (1305), Expect = 4.5e-140
Identity = 236/310 (76.13%), Postives = 272/310 (87.74%), Query Frame = 0

Query: 3   MDQIQHNFIQVRGLKLHVAEIGTGSNVVVFLHGFPEIWYSWRHQMIALANAGFRAIAPDF 62
           MD+I HNFIQV  LKLHVAEIGTGSNVVVFLHGFPEIWYSWR+QMIALA+AGFR +APD+
Sbjct: 1   MDRIHHNFIQVGALKLHVAEIGTGSNVVVFLHGFPEIWYSWRYQMIALADAGFRVLAPDY 60

Query: 63  RGYGLSDAPAEPSKVEFSDLVSDLLGILDALHIPKVFLVAKDFGARPAFCFAIKHQRRVL 122
           RGYGLSD+PAEPSK  FSDL+SDLLGILDAL+IPKVF+VAKDFGA PA+ FA+KH  R L
Sbjct: 61  RGYGLSDSPAEPSKASFSDLISDLLGILDALNIPKVFVVAKDFGAWPAYYFALKHPERAL 120

Query: 123 GVVTLGVPFIPPGPHK-----FPQGFYISRWQEPRRAEADFGRFDAKTIIRIVYILFSKS 182
           G+VTLGVPF+PP   K      P+G Y  RW+EP RAEADFGRFDAKT++R VYILFSKS
Sbjct: 121 GIVTLGVPFLPPESLKHSQSNIPEGVYTLRWREPGRAEADFGRFDAKTVVRNVYILFSKS 180

Query: 183 EIPVAQENQEIMDLVDPSSPLPPWLSEEDLITYGALYEKSGFSTALKVPYRSLNEDWGIQ 242
           EIP AQENQE+MDLVDPS+PLPPW +EEDL TYGALYEKSGF TALKVPYRS NEDWGI+
Sbjct: 181 EIPTAQENQEVMDLVDPSTPLPPWFTEEDLATYGALYEKSGFDTALKVPYRSFNEDWGIK 240

Query: 243 DPKVKVPTMLIMGEKDYTRKFPNIDEYIQSGKMEEYVPNLEIIYLPEGSHFVQEQCPKKV 302
           DP+V++P + IMGEKDY  KFP +DEY++S +++++VPNLEI+YLPEGSHFVQEQ P++V
Sbjct: 241 DPRVEIPALFIMGEKDYVFKFPEVDEYVRSERVKDFVPNLEIVYLPEGSHFVQEQSPEEV 300

Query: 303 NELLLTFLAK 308
           N LLLTFLAK
Sbjct: 301 NHLLLTFLAK 310

BLAST of Spg011737 vs. ExPASy TrEMBL
Match: A0A6J1CM01 (bifunctional epoxide hydrolase 2-like OS=Momordica charantia OX=3673 GN=LOC111012513 PE=4 SV=1)

HSP 1 Score: 504.2 bits (1297), Expect = 3.8e-139
Identity = 234/312 (75.00%), Postives = 275/312 (88.14%), Query Frame = 0

Query: 3   MDQIQHNFIQVRG-LKLHVAEIGTGSNVVVFLHGFPEIWYSWRHQMIALANAGFRAIAPD 62
           MDQIQH F+QV+G L++HVA+IGTGSNVV+FLHGFPEIWYSWRHQMIALANAGFRAIAPD
Sbjct: 1   MDQIQHVFVQVQGALQIHVAQIGTGSNVVLFLHGFPEIWYSWRHQMIALANAGFRAIAPD 60

Query: 63  FRGYGLSDAPAEPSKVEFSDLVSDLLGILDALHIPKVFLVAKDFGARPAFCFAIKHQRRV 122
           +RGYGLSDAPAEPSK  ++DL+ DLL ILD LHIPKVFLV KDFGA PA+ FA+KH  R 
Sbjct: 61  YRGYGLSDAPAEPSKATYADLIGDLLQILDTLHIPKVFLVGKDFGAMPAYYFALKHPERA 120

Query: 123 LGVVTLGVPFIPPGPHKF----PQGFYISRWQEPRRAEADFGRFDAKTIIRIVYILFSKS 182
           LGVVTLGVPF+PP P KF    P+GFYISRWQEP RAE+DFGRFDAKT++R +YILFS+S
Sbjct: 121 LGVVTLGVPFMPPEPIKFQSHLPEGFYISRWQEPGRAESDFGRFDAKTVVRNIYILFSRS 180

Query: 183 EIPVAQENQEIMDLVDPSSPLPPWLSEEDLITYGALYEKSGFSTALKVPYRSLNEDWGIQ 242
           E+P+A+ENQEIMDLVD S+PLPPW +EEDL  YG LY+KSGF TALKVPYRSL EDWGI+
Sbjct: 181 EVPIAEENQEIMDLVDSSTPLPPWFTEEDLAAYGDLYKKSGFDTALKVPYRSLGEDWGIK 240

Query: 243 DPKVKVPTMLIMGEKDYTRKFPNIDEYIQSGKMEEYVPNLEIIYLPEGSHFVQEQCPKKV 302
           DPKV++P +LIMGEKDY  KFP I+EY++S K+++YVP LEIIYL EGSHFVQEQ P+++
Sbjct: 241 DPKVEIPALLIMGEKDYVLKFPGIEEYVRSEKVKDYVPKLEIIYLAEGSHFVQEQSPEEI 300

Query: 303 NELLLTFLAKNT 310
           N+LLL FL+K+T
Sbjct: 301 NQLLLNFLSKHT 312

BLAST of Spg011737 vs. TAIR 10
Match: AT4G02340.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 220.3 bits (560), Expect = 2.1e-57
Identity = 120/319 (37.62%), Postives = 177/319 (55.49%), Query Frame = 0

Query: 3   MDQIQHNFIQVRGLKLHVAEIGTGSNVVVFLHGFPEIWYSWRHQMIALANAGFRAIAPDF 62
           M++I+H  I   G+ +HVA IG+G  V++F+HGFP++WYSWRHQ+++ A  G+RAIAPD 
Sbjct: 1   MEKIEHTTISTNGINMHVASIGSGP-VILFVHGFPDLWYSWRHQLVSFAALGYRAIAPDL 60

Query: 63  RGYGLSDAPAEPSKVEFSDLVSDLLGILDALHIPKVFLVAKDFGARPAFCFAIKHQRRVL 122
           RGYG SDAP          +V DL+G+LD+L + +VFLV  D+GA  A+   +    RV 
Sbjct: 61  RGYGDSDAPPSRESYTILHIVGDLVGLLDSLGVDRVFLVGHDWGAIVAWWLCMIRPDRVN 120

Query: 123 GVVTLGVPFIPPGPH---------KFPQGFYISRWQEPRRAEADFGRFDAKTIIRIVYIL 182
            +V   V F P  P           F   +YI R+QEP   E DF + D K +I   +  
Sbjct: 121 ALVNTSVVFNPRNPSVKPVDAFRALFGDDYYICRFQEPGEIEEDFAQVDTKKLI-TRFFT 180

Query: 183 FSKSEIPVAQENQEIMDLVDPSSPLPPWLSEEDLITYGALYEKSGFSTALKVPYRSLNED 242
                 P   ++     L DP S LP WL+E+D+  YG  + + GF+  L   YR+LN  
Sbjct: 181 SRNPRPPCIPKSVGFRGLPDPPS-LPAWLTEQDVRFYGDKFSQKGFTGGLNY-YRALNLS 240

Query: 243 WGIQDP----KVKVPTMLIMGEKDYTRKFPNIDEYIQSGKMEEYVPNLEIIYLPEG-SHF 302
           W +  P    ++KVP   I+G+ D T   P   EYI  G ++++VP L+ + + EG  HF
Sbjct: 241 WELTAPWTGLQIKVPVKFIVGDLDITYNIPGTKEYIHEGGLKKHVPFLQEVVVMEGVGHF 300

Query: 303 VQEQCPKKVNELLLTFLAK 308
           + ++ P +V + +  F  K
Sbjct: 301 LHQEKPDEVTDHIYGFFKK 315

BLAST of Spg011737 vs. TAIR 10
Match: AT3G51000.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 214.9 bits (546), Expect = 8.9e-56
Identity = 114/316 (36.08%), Postives = 187/316 (59.18%), Query Frame = 0

Query: 6   IQHNFIQVRGLKLHVAEIGTGSN-VVVFLHGFPEIWYSWRHQMIALANAGFRAIAPDFRG 65
           ++   I+  G+ L+VAE G     +V+ LHGFPE WYSWRHQ+  L++ G+  +APD RG
Sbjct: 5   VREKKIKTNGIWLNVAEKGDEEGPLVLLLHGFPETWYSWRHQIDFLSSHGYHVVAPDLRG 64

Query: 66  YGLSDAPAEPSKVEFSDLVSDLLGILDALHIPKVFLVAKDFGARPAFCFAIKHQRRVLGV 125
           YG SD+         S LV+D++G+LD     + F+   D+GA   +C  +    RV G 
Sbjct: 65  YGDSDSLPSHESYTVSHLVADVIGLLDHYGTTQAFVAGHDWGAIIGWCLCLFRPDRVKGF 124

Query: 126 VTLGVPFIPPGPHK--------FPQGFYISRWQEPRRAEADFGRFDAKTIIRIVYILFSK 185
           ++L VP+ P  P          F  G YI+++Q+P RAEA F + D  ++++  ++L ++
Sbjct: 125 ISLSVPYFPRDPKLKPSDFFKIFGDGLYITQFQKPGRAEAAFAKHDCLSVMK-KFLLITR 184

Query: 186 SEIPVAQENQEIMDLVDPSSPLPPWLSEEDLITYGALYEKSGFSTALKVPYRSLNEDWGI 245
           ++  VA  + EI+D ++  S +P W++EE++  Y   +++SGF+  L   YRS++ +W I
Sbjct: 185 TDYLVAPPDTEIIDHLEIPSTIPDWITEEEIQVYAEKFQRSGFTGPLNY-YRSMDMNWEI 244

Query: 246 ----QDPKVKVPTMLIMGEKDYTRKFPN-IDEYIQSGKMEEYVPNLEIIYLPEGSHFVQE 305
               QD K+ VPT  I G+KD   + PN   EY++    +  VPNLEI+ +  G HF+Q+
Sbjct: 245 LAPWQDSKIVVPTKFIAGDKDIGYEGPNGTMEYVKGEVFKIVVPNLEIVVIEGGHHFIQQ 304

Query: 306 QCPKKVNELLLTFLAK 308
           +  ++V++ +L+FL K
Sbjct: 305 EKSEQVSQEILSFLNK 318

BLAST of Spg011737 vs. TAIR 10
Match: AT3G05600.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 190.3 bits (482), Expect = 2.4e-48
Identity = 110/325 (33.85%), Postives = 177/325 (54.46%), Query Frame = 0

Query: 3   MDQIQHNFIQVRGLKLHVAEIGTGSN-VVVFLHGFPEIWYSWRHQMIALANAGFRAIAPD 62
           M+ I H  + V G+ +H+AE G     VV+ LHGFP++WY+WRHQ+  L++ G+RA+APD
Sbjct: 1   MEGIDHRMVSVNGITMHIAEKGPKEGPVVLLLHGFPDLWYTWRHQISGLSSLGYRAVAPD 60

Query: 63  FRGYGLSDAPAEPSKVEFSDLVSDLLGILDAL--HIPKVFLVAKDFGARPAFCFAIKHQR 122
            RGYG SD+P   S+    ++V DL+ +LD++  +  KVFLV  D+GA   +   +    
Sbjct: 61  LRGYGDSDSPESFSEYTCLNVVGDLVALLDSVAGNQEKVFLVGHDWGAIIGWFLCLFRPE 120

Query: 123 RVLGVVTLGVPFIPPGPHKFP-QGF--------YISRWQEPRRAEADFGRFDAKTIIRIV 182
           ++ G V L VP+    P   P QGF        YI R+QEP + E +    D +  +R +
Sbjct: 121 KINGFVCLSVPYRSRNPKVKPVQGFKAVFGDDYYICRFQEPGKIEGEIASADPRIFLRNL 180

Query: 183 YILFSKSEIPVAQENQEIMDLVDPSS---PLPPWLSEEDLITYGALYEKSGFSTALKVPY 242
           +   +    P+  ++    +  +P+S    LP W S++DL  Y + +EK+GF+  L   Y
Sbjct: 181 FTGRTLGP-PILPKDNPFGEKPNPNSENIELPEWFSKKDLDFYVSKFEKAGFTGGLNY-Y 240

Query: 243 RSLNEDWGIQDP----KVKVPTMLIMGEKDYTRKFPNIDEYIQSGKMEEYVPNL-EIIYL 302
           R+++ +W +  P    K++VP   + G+ D     P + EYI  G     VP L EI+ +
Sbjct: 241 RAMDLNWELTAPWTGAKIQVPVKFMTGDFDMVYTTPGMKEYIHGGGFAADVPTLQEIVVI 300

Query: 303 PEGSHFVQEQCPKKVNELLLTFLAK 308
            +  HFV ++ P++V   +  F  K
Sbjct: 301 EDAGHFVNQEKPQEVTAHINDFFTK 323

BLAST of Spg011737 vs. TAIR 10
Match: AT2G26740.1 (soluble epoxide hydrolase )

HSP 1 Score: 187.6 bits (475), Expect = 1.5e-47
Identity = 109/322 (33.85%), Postives = 176/322 (54.66%), Query Frame = 0

Query: 6   IQHNFIQVRGLKLHVAEIGTGSN-VVVFLHGFPEIWYSWRHQMIALANAGFRAIAPDFRG 65
           ++H  ++  G+ +HVA  G     +V+ LHGFPE+WYSWRHQ+  LA  G+RA+APD RG
Sbjct: 1   MEHRKVRGNGIDIHVAIQGPSDGPIVLLLHGFPELWYSWRHQIPGLAARGYRAVAPDLRG 60

Query: 66  YGLSDAPAEPSKVEFSDLVSDLLGILDAL---HIPKVFLVAKDFGARPAFCFAIKHQRRV 125
           YG SDAPAE S     ++V DL+ ++ AL      KVF+V  D+GA  A+   +    RV
Sbjct: 61  YGDSDAPAEISSYTCFNIVGDLIAVISALTASEDEKVFVVGHDWGALIAWYLCLFRPDRV 120

Query: 126 LGVVTLGVPFI--PPGPHKFP---------QGFYISRWQEPRRAEADFGRFDAKTIIRIV 185
             +V L VPF   P  P   P           +YI R+QE    EA+      + +++ +
Sbjct: 121 KALVNLSVPFSFRPTDPSVKPVDRMRAFYGDDYYICRFQEFGDVEAEIAEVGTERVMKRL 180

Query: 186 YILFSKSEIPVAQENQEIMDLVDPSSPLPPWLSEEDLITYGALYEKSGFSTALKVPYRSL 245
               +   + +  +++        + PLP WL+EED+  + + +E+ GFS  +   YR+ 
Sbjct: 181 LTYRTPGPV-IIPKDKSFWGSKGETIPLPSWLTEEDVAYFVSKFEEKGFSGPVNY-YRNF 240

Query: 246 NEDWGIQDP----KVKVPTMLIMGEKDYTRKFPNIDEYIQSGKMEEYVPNLEIIYLPEG- 305
           N +  +  P    K++VPT  ++GE D     P + EYI   + +E VP LE   + EG 
Sbjct: 241 NRNNELLGPWVGSKIQVPTKFVIGELDLVYYMPGVKEYIHGPQFKEDVPLLEEPVVMEGV 300

Query: 306 SHFVQEQCPKKVNELLLTFLAK 308
           +HF+ ++ P+++ +++L F++K
Sbjct: 301 AHFINQEKPQEILQIILDFISK 320

BLAST of Spg011737 vs. TAIR 10
Match: AT4G15960.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 187.2 bits (474), Expect = 2.0e-47
Identity = 106/326 (32.52%), Postives = 178/326 (54.60%), Query Frame = 0

Query: 3   MDQIQHNFIQVRGLKLHVAE-IGTGSN---VVVFLHGFPEIWYSWRHQMIALANAGFRAI 62
           +D ++H  ++V G+ +HVAE  G+GS    +++FLHGFPE+WY+WRHQM+AL++ G+R I
Sbjct: 51  LDGVEHKTLKVNGINMHVAEKPGSGSGEDPIILFLHGFPELWYTWRHQMVALSSLGYRTI 110

Query: 63  APDFRGYGLSDAPAEPSKVEFSDLVSDLLGILDAL--HIPKVFLVAKDFGARPAFCFAIK 122
           APD RGYG ++AP +     + ++  D++ ++DA+      V +V  D+GA  A+     
Sbjct: 111 APDLRGYGDTEAPEKVEDYTYLNVDGDVVALIDAVTGGDKAVSVVGHDWGAMIAWQLCQY 170

Query: 123 HQRRVLGVVTLGVPFIPPGP---------HKFPQGFYISRWQEPRRAEADFGRFDAKTII 182
              +V  +V + V F P  P         H F   +Y+ R+Q+    E +F +   + ++
Sbjct: 171 RPEKVKALVNMSVLFSPRNPVRVPVPTLRHVFGDDYYVCRFQKAGEIETEFKKLGTENVL 230

Query: 183 RIVYILFSKSEIPV-AQENQEIMDLVDPSSPLPPWLSEEDLITYGALYEKSGFSTALKVP 242
           +    L  K+  P+   +++      + +S LP WL++EDL  Y   YE  GF+  +   
Sbjct: 231 K--EFLTYKTPGPLNLPKDKYFKRSENAASALPLWLTQEDLDYYVTKYENKGFTGPINY- 290

Query: 243 YRSLNEDWGIQDP----KVKVPTMLIMGEKDYTRKFPNIDEYIQSGKMEEYVPNL-EIIY 302
           YR+++ +W +  P    K++VP   I+G++D T  FP   EYI  G  +  VP L E + 
Sbjct: 291 YRNIDRNWELTAPWTGAKIRVPVKFIIGDQDLTYNFPGAKEYINGGGFKRDVPLLDETVV 350

Query: 303 LPEGSHFVQEQCPKKVNELLLTFLAK 308
           L    HF+ E+ P  +N+ +  F  K
Sbjct: 351 LKGLGHFLHEENPDVINQHIHNFFHK 373

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022950359.14.5e-14276.85uncharacterized protein LOC111453471 [Cucurbita moschata][more]
XP_023544288.15.9e-14277.17uncharacterized protein LOC111803907 [Cucurbita pepo subsp. pepo][more]
XP_022142308.15.9e-14276.83bifunctional epoxide hydrolase 2-like isoform X2 [Momordica charantia][more]
XP_022978890.12.2e-14177.17uncharacterized protein LOC111478707 [Cucurbita maxima][more]
XP_008439884.29.4e-14076.13PREDICTED: bifunctional epoxide hydrolase 2-like [Cucumis melo][more]
Match NameE-valueIdentityDescription
I6YGS09.4e-3431.01Epoxide hydrolase A OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX... [more]
P349133.6e-3329.56Bifunctional epoxide hydrolase 2 OS=Homo sapiens OX=9606 GN=EPHX2 PE=1 SV=2[more]
P349147.9e-3330.19Bifunctional epoxide hydrolase 2 OS=Mus musculus OX=10090 GN=Ephx2 PE=1 SV=2[more]
P802991.8e-3229.41Bifunctional epoxide hydrolase 2 OS=Rattus norvegicus OX=10116 GN=Ephx2 PE=1 SV=... [more]
Q6Q2C23.3e-3129.56Bifunctional epoxide hydrolase 2 OS=Sus scrofa OX=9823 GN=EPHX2 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1GFK22.2e-14276.85uncharacterized protein LOC111453471 OS=Cucurbita moschata OX=3662 GN=LOC1114534... [more]
A0A6J1CMZ32.8e-14276.83bifunctional epoxide hydrolase 2-like isoform X2 OS=Momordica charantia OX=3673 ... [more]
A0A6J1IRI31.1e-14177.17uncharacterized protein LOC111478707 OS=Cucurbita maxima OX=3661 GN=LOC111478707... [more]
A0A1S3AZS94.5e-14076.13bifunctional epoxide hydrolase 2-like OS=Cucumis melo OX=3656 GN=LOC103484538 PE... [more]
A0A6J1CM013.8e-13975.00bifunctional epoxide hydrolase 2-like OS=Momordica charantia OX=3673 GN=LOC11101... [more]
Match NameE-valueIdentityDescription
AT4G02340.12.1e-5737.62alpha/beta-Hydrolases superfamily protein [more]
AT3G51000.18.9e-5636.08alpha/beta-Hydrolases superfamily protein [more]
AT3G05600.12.4e-4833.85alpha/beta-Hydrolases superfamily protein [more]
AT2G26740.11.5e-4733.85soluble epoxide hydrolase [more]
AT4G15960.12.0e-4732.52alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000639Epoxide hydrolase-likePRINTSPR00412EPOXHYDRLASEcoord: 34..52
score: 55.5
coord: 54..69
score: 56.53
coord: 282..304
score: 24.31
coord: 243..259
score: 31.02
IPR000073Alpha/beta hydrolase fold-1PFAMPF00561Abhydrolase_1coord: 28..253
e-value: 2.7E-21
score: 76.4
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 3..308
e-value: 8.5E-84
score: 284.0
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 3..307
NoneNo IPR availablePANTHERPTHR43329EPOXIDE HYDROLASEcoord: 4..308
NoneNo IPR availablePANTHERPTHR43329:SF106BIFUNCTIONAL EPOXIDE HYDROLASE 2-LIKE ISOFORM X1coord: 4..308

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg011737.1Spg011737.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016787 hydrolase activity
molecular_function GO:0003824 catalytic activity