Spg011623 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg011623
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Vacuolar protein sorting-associated protein 28 homolog
Location	scaffold1: 3368941 .. 3371984 (+)
RNA-Seq Expression	Spg011623
Synteny	Spg011623

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGGTCAAGCTTTGGAACGACAAGCGCGAGAGAGAAATGTACGACAATTTTGCAGAGCTCTACGCCATTATCAAGGCCACCGAAAAGCTCGAAAAGGCTTACATCCGCGACATAATCCCCTCTTCCGATTACGAGACCGAATGCTTCAAACTCATCGCACATTTCAAAACCTTGGCCTCGACGCTCAAAGACACAGTCCCCAGCATTGAACGATTCGCAGACACTTACAAAATGGACTGTCCGGCGGCGATTAACCGTCTTGTGAACTCCGGTGTCCCTGCCACAGTGGAGCACAGGGCGGCTGCCGCCGCCTCAGCCACCACCTCCGCTGCCATTGTTGCGGAGTGTGTGCAGAACTTCATTACGGCTATGGATTCGTTGAAACTGAACATGGTGGCGGTGGATCAGGTGCACCCCCTGCTCTCTGATCTCTCTGCTTCACTGAATAAATTGACCATTCTGCCGCCGGATTTCGAAGGGAAGACAAAGATGAGGGAGTGGCTTGGACGGCTTTCGAAAATGGGCGCTGCGGATGAGTTAACGGAGCAGCAGGCTAGGCAGCTTCACTTTGATCTTGAATCTTCTTATAATTCATTCATGGCAGCCTTGCCTACTGCTGGTACTTGAATGTTATTGGTAATCTTCTGTATATACATAAGAGATTAATGATTCTATCGTACTGCATAAGTTCGCTTTCTTCATAAATCAAGATGGGATGTGCTTTTTTTTTCCTTTTCAATATGGATGCCTATTGAAGAAGATGAACTTTTTTTGTTTCCTTTTTCTTATTCTCACATTCAGTTTTTTTTTTTTTTTTTTTTTTTGAAAATAAAAACCATAAAAACCATTATTCTCACATTCAGTTTGAGAGAGCTTGTGATGATCTTACATTGTTGATTTAGATTGTTTGGAAACTGGTGTACTGAGTTGTGTATGTAATTCTGATTGTTGTGAGAAAAATTTTGGTTTTGGAAACAGATGAAAGTGGAAACTACACTGCAATGCACTAAGAACTACATCTCTTTTTTGGGGCACTTTGGGGGTTTTTGTGGGGTTGGATTTTTCGTGGGTTAGGTTTGGTTTAATGCTTGACCCATGATTTTGAGGGCATCGGTAGATTTGTTCTATTAGGCTGTCACTTCCATATTCATGGGTTTGAAGAGTGCTTTATTCAGAAACCTACTTCATTTCACCAAAACATTGTTTGGAAACTGGTACTGAAATATGCATGTGATTCTGATTGTTGTAAGAAAAGTTTTGGTTTGGAACCAGAGAAAAGTGAAAGTTGCTGCTATGCTGCAGCAGGAGCTATATCTGTATCTGATGCCCAAATCCTTTTTTGGGTACTTTTGTGGTATGTGGGGTTCGATTCGTGACTTTTGGGTTTTCAGTTCCAGAACTCATGGGGTTACATTGTAAGAGTTCTTTATTCAGAAACTTACCTCATTGGTCAGCTTTCTGCTCACAATACTTGTCCTCAATGCCGAGTTTTTCTTATGAGTGTTTGAAGTAACCATATCCCTTGGATAAAGCAAGTCAATCTAAAATCATGGCAAGCATCTTTGAAAATCTTGGCCTTTGGGATTTGCTTATATGGAATTCACGAGCTTTCCAGTAGTAGGTTGAAAGAAGTTGGGATGTCTAGTATCGAGAATTTTTTCTTAATTGGTACCAACTTTGTAGTTGATTATAAGGTCAGAATGTCCTGAGTATACCCCAATGTGTCATATGCTCCAATGGCCCATGTCAATAATGAAATGTTTTGCTATACCCACATGTCTTTTGGAAGTATATTTGTGCATTTTCTCTTTATGTTTTTTAAATAGATTTATCTTGTTGGAGAATTGTGATTAAAATAAAACGAATTCAAATCTATTGTCATTTATTATAGAAAAAAAATTGACTCTGTTGAATATCATCTAGAGGAGATAAAAACCAATATATTGAAATTTTAATATGTAAATACAATCAACTATAGTTTGAAATAACATTGAATTTCCAAATTAATCCAATTAAACATTTTCAAACTCCAATCCTCGGAAGCTATGACCTAAATACCTATTTCCTAAGCTCATACTTTAATTCATAACAAACAAAGACAACTGATAGATGTCATTGTGCCAATGGCTCTGAGCTAATTTGGTTGGGTTGGATTTGATTGAATTGAGTTGTAAATTTGTAATTAACATAGATTCATATGATAAAAGTTATTGGATTTCGTTTCAAACACTCAACCTCTCCATAAAGGCACTTACATGCGACAATTCAAACTAGTATCTGATCTGACAGGACTATGGTAGAGCCTCCTACTTGATTCTTGGACTAGATTAGACTTAAGGATAAGTTTAATTTGGATGAGGTAGAACATGATCATCATAGAGATGGTTTCTTTCGTGATTATGAGTTTCGTTTTGATCATATCGATGAATTGGAATATGACATGAGATAAAGACGTGATCTGCTAAAGTAAGAACAATTCAACGATAATTGGTATGTGCCTTTGAAATCAAAGGTTTGAATCTCTCATCTCAAAATATTGTTGCATTTGAAAACTATATACCACTTTATGATGCAAATGAGTCATATGCTTTCTTCTTCTCGTGTCCGCATGAAATTTCAATCATGTGATTCAAGGAAAAACCTCCCGGATTTGATTTCTAGCAAGAAATATCTACTGCTTTTTATGGTTTTTATTATTTGTTCCAGGTGGAGCATAAAGAACGAGAGAGAAACTTTTTCTGTTTGTAGAAAAATTATTTGGGGAGACCGCCCGCCTCAATGTAACCCTCCCTCAATAAGATAAAGTCAGAACTCTTTAGCATTTGATCCTTTTCTTACCATGGAAGTTTTAAATCACCGTCATCCTATTTGTTATCCTAAGAAAACAAGAAAAAAAGAAATATGTCCCGTATTTCAACTTCAATTAAATTGAGGAATCTCTGTAGCGGGAAAAACTGATAAGTCCTGGTCCAGATGGGGCAGATTCCATTGCAATGAGGTGCCCAAGTTCAATAATTGATGCAGCAAAAGTGAAAGGATAA

mRNA sequence

ATGGAGGTCAAGCTTTGGAACGACAAGCGCGAGAGAGAAATGTACGACAATTTTGCAGAGCTCTACGCCATTATCAAGGCCACCGAAAAGCTCGAAAAGGCTTACATCCGCGACATAATCCCCTCTTCCGATTACGAGACCGAATGCTTCAAACTCATCGCACATTTCAAAACCTTGGCCTCGACGCTCAAAGACACAGTCCCCAGCATTGAACGATTCGCAGACACTTACAAAATGGACTGTCCGGCGGCGATTAACCGTCTTGTGAACTCCGGTGTCCCTGCCACAGTGGAGCACAGGGCGGCTGCCGCCGCCTCAGCCACCACCTCCGCTGCCATTGTTGCGGAGTGTGTGCAGAACTTCATTACGGCTATGGATTCGTTGAAACTGAACATGGTGGCGGTGGATCAGGTGCACCCCCTGCTCTCTGATCTCTCTGCTTCACTGAATAAATTGACCATTCTGCCGCCGGATTTCGAAGGGAAGACAAAGATGAGGGAGTGGCTTGGACGGCTTTCGAAAATGGGCGCTGCGGATGAGTTAACGGAGCAGCAGGCTAGGCAGCTTCACTTTGATCTTGAATCTTCTTATAATTCATTCATGGCAGCCTTGCCTACTGCTGGCTGTCACTTCCATATTCATGGGTTTGAAGAGTGCTTTATTCAGAAACCTACTTCATTTCACCAAAACATTGTTTGGAAACTGGTACTGAAATATGCATGTGATTCTGATTGTTGTAAGAAAAGTTTTGGTTTGGAACCAGAGAAAAGTGAAAGTTGCTGCTATGCTGCAGCAGGAGCTATATCTGTATCTGATGCCCAAATCCTTTTTTGGCGGGAAAAACTGATAAGTCCTGGTCCAGATGGGGCAGATTCCATTGCAATGAGGTGCCCAAGTTCAATAATTGATGCAGCAAAAGTGAAAGGATAA

Coding sequence (CDS)

ATGGAGGTCAAGCTTTGGAACGACAAGCGCGAGAGAGAAATGTACGACAATTTTGCAGAGCTCTACGCCATTATCAAGGCCACCGAAAAGCTCGAAAAGGCTTACATCCGCGACATAATCCCCTCTTCCGATTACGAGACCGAATGCTTCAAACTCATCGCACATTTCAAAACCTTGGCCTCGACGCTCAAAGACACAGTCCCCAGCATTGAACGATTCGCAGACACTTACAAAATGGACTGTCCGGCGGCGATTAACCGTCTTGTGAACTCCGGTGTCCCTGCCACAGTGGAGCACAGGGCGGCTGCCGCCGCCTCAGCCACCACCTCCGCTGCCATTGTTGCGGAGTGTGTGCAGAACTTCATTACGGCTATGGATTCGTTGAAACTGAACATGGTGGCGGTGGATCAGGTGCACCCCCTGCTCTCTGATCTCTCTGCTTCACTGAATAAATTGACCATTCTGCCGCCGGATTTCGAAGGGAAGACAAAGATGAGGGAGTGGCTTGGACGGCTTTCGAAAATGGGCGCTGCGGATGAGTTAACGGAGCAGCAGGCTAGGCAGCTTCACTTTGATCTTGAATCTTCTTATAATTCATTCATGGCAGCCTTGCCTACTGCTGGCTGTCACTTCCATATTCATGGGTTTGAAGAGTGCTTTATTCAGAAACCTACTTCATTTCACCAAAACATTGTTTGGAAACTGGTACTGAAATATGCATGTGATTCTGATTGTTGTAAGAAAAGTTTTGGTTTGGAACCAGAGAAAAGTGAAAGTTGCTGCTATGCTGCAGCAGGAGCTATATCTGTATCTGATGCCCAAATCCTTTTTTGGCGGGAAAAACTGATAAGTCCTGGTCCAGATGGGGCAGATTCCATTGCAATGAGGTGCCCAAGTTCAATAATTGATGCAGCAAAAGTGAAAGGATAA

Protein sequence

MEVKLWNDKREREMYDNFAELYAIIKATEKLEKAYIRDIIPSSDYETECFKLIAHFKTLASTLKDTVPSIERFADTYKMDCPAAINRLVNSGVPATVEHRAAAAASATTSAAIVAECVQNFITAMDSLKLNMVAVDQVHPLLSDLSASLNKLTILPPDFEGKTKMREWLGRLSKMGAADELTEQQARQLHFDLESSYNSFMAALPTAGCHFHIHGFEECFIQKPTSFHQNIVWKLVLKYACDSDCCKKSFGLEPEKSESCCYAAAGAISVSDAQILFWREKLISPGPDGADSIAMRCPSSIIDAAKVKG

Homology

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BLAST of Spg011623 vs. NCBI nr
Match: XP_022142298.1 (vacuolar protein sorting-associated protein 28 homolog 2 [Momordica charantia] >XP_022142299.1 vacuolar protein sorting-associated protein 28 homolog 2 [Momordica charantia])

HSP 1 Score: 401.0 bits (1029), Expect = 9.4e-108
Identity = 207/208 (99.52%), Postives = 207/208 (99.52%), Query Frame = 0


Query: 1   MEVKLWNDKREREMYDNFAELYAIIKATEKLEKAYIRDIIPSSDYETECFKLIAHFKTLA 60
           MEVKLWNDKREREMYDNFAELYAIIKATEKLEKAYIRDIIPSSDYETECFKLIAHFKTLA
Sbjct: 1   MEVKLWNDKREREMYDNFAELYAIIKATEKLEKAYIRDIIPSSDYETECFKLIAHFKTLA 60

Query: 61  STLKDTVPSIERFADTYKMDCPAAINRLVNSGVPATVEHRAAAAASATTSAAIVAECVQN 120
           STLKDTVPSIERFADTYKMDCPAAINRLVNSGVPATVEHRAAAAASATTSAAIVAECVQN
Sbjct: 61  STLKDTVPSIERFADTYKMDCPAAINRLVNSGVPATVEHRAAAAASATTSAAIVAECVQN 120

Query: 121 FITAMDSLKLNMVAVDQVHPLLSDLSASLNKLTILPPDFEGKTKMREWLGRLSKMGAADE 180
           FITAMDSLKLNMVAVDQVHPLLSDLSASLNKLTILP DFEGKTKMREWLGRLSKMGAADE
Sbjct: 121 FITAMDSLKLNMVAVDQVHPLLSDLSASLNKLTILPADFEGKTKMREWLGRLSKMGAADE 180

Query: 181 LTEQQARQLHFDLESSYNSFMAALPTAG 209
           LTEQQARQLHFDLESSYNSFMAALPTAG
Sbjct: 181 LTEQQARQLHFDLESSYNSFMAALPTAG 208

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BLAST of Spg011623 vs. NCBI nr
Match: XP_038883329.1 (vacuolar protein sorting-associated protein 28 homolog 2 [Benincasa hispida] >XP_038883330.1 vacuolar protein sorting-associated protein 28 homolog 2 [Benincasa hispida])

HSP 1 Score: 395.2 bits (1014), Expect = 5.2e-106
Identity = 204/208 (98.08%), Postives = 206/208 (99.04%), Query Frame = 0


Query: 1   MEVKLWNDKREREMYDNFAELYAIIKATEKLEKAYIRDIIPSSDYETECFKLIAHFKTLA 60
           MEVKLWNDKREREMYDNFAELYAIIKATEKLEKAYIRDII SSDYETECFKLIAHFKTLA
Sbjct: 1   MEVKLWNDKREREMYDNFAELYAIIKATEKLEKAYIRDIISSSDYETECFKLIAHFKTLA 60

Query: 61  STLKDTVPSIERFADTYKMDCPAAINRLVNSGVPATVEHRAAAAASATTSAAIVAECVQN 120
           STLKDTVPSIERFADTYKMDCPAAINRLV +GVPATVEHRAAAAASATTSAAIVAECVQN
Sbjct: 61  STLKDTVPSIERFADTYKMDCPAAINRLVITGVPATVEHRAAAAASATTSAAIVAECVQN 120

Query: 121 FITAMDSLKLNMVAVDQVHPLLSDLSASLNKLTILPPDFEGKTKMREWLGRLSKMGAADE 180
           FITAMDSLKLNMVAVDQVHPLLSDLSASLNKLTILPPDFEGKTKM+EWLGRLSKMGAADE
Sbjct: 121 FITAMDSLKLNMVAVDQVHPLLSDLSASLNKLTILPPDFEGKTKMKEWLGRLSKMGAADE 180

Query: 181 LTEQQARQLHFDLESSYNSFMAALPTAG 209
           LTEQQARQLHFDLESSYNSFMAALPTAG
Sbjct: 181 LTEQQARQLHFDLESSYNSFMAALPTAG 208

[Close]

BLAST of Spg011623 vs. NCBI nr
Match: XP_023544381.1 (vacuolar protein sorting-associated protein 28 homolog 2 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023544382.1 vacuolar protein sorting-associated protein 28 homolog 2 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 394.4 bits (1012), Expect = 8.8e-106
Identity = 202/208 (97.12%), Postives = 206/208 (99.04%), Query Frame = 0


Query: 1   MEVKLWNDKREREMYDNFAELYAIIKATEKLEKAYIRDIIPSSDYETECFKLIAHFKTLA 60
           MEVKLWNDKREREMYDNFAELYAIIKATEKLEKAY+RDIIPSSDYETECFKLIAHFKTLA
Sbjct: 37  MEVKLWNDKREREMYDNFAELYAIIKATEKLEKAYVRDIIPSSDYETECFKLIAHFKTLA 96

Query: 61  STLKDTVPSIERFADTYKMDCPAAINRLVNSGVPATVEHRAAAAASATTSAAIVAECVQN 120
           STLKDTVPSIERFADTYKMDCPAAINRLV SGVPATVEHRA+AAAS+TTSAAIVAECVQN
Sbjct: 97  STLKDTVPSIERFADTYKMDCPAAINRLVISGVPATVEHRASAAASSTTSAAIVAECVQN 156

Query: 121 FITAMDSLKLNMVAVDQVHPLLSDLSASLNKLTILPPDFEGKTKMREWLGRLSKMGAADE 180
           FITAMDSLKLNMVAVDQVHPLLSDL ASLNKLTILPPDFEGKTKM+EWLGRLSKMGAADE
Sbjct: 157 FITAMDSLKLNMVAVDQVHPLLSDLFASLNKLTILPPDFEGKTKMKEWLGRLSKMGAADE 216

Query: 181 LTEQQARQLHFDLESSYNSFMAALPTAG 209
           LTEQQARQLHFDLESSYNSFMAALPTAG
Sbjct: 217 LTEQQARQLHFDLESSYNSFMAALPTAG 244

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BLAST of Spg011623 vs. NCBI nr
Match: KAG6603719.1 (Vacuolar protein sorting-associated protein 28-like 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 394.4 bits (1012), Expect = 8.8e-106
Identity = 205/222 (92.34%), Postives = 211/222 (95.05%), Query Frame = 0


Query: 1   MEVKLWNDKREREMYDNFAELYAIIKATEKLEKAYIRDIIPSSDYETECFKLIAHFKTLA 60
           MEVKLWNDKREREMYDNFAELYAIIKATEKLEKAY+RDIIPSSDYETECFKLIAHFKTLA
Sbjct: 1   MEVKLWNDKREREMYDNFAELYAIIKATEKLEKAYVRDIIPSSDYETECFKLIAHFKTLA 60

Query: 61  STLKDTVPSIERFADTYKMDCPAAINRLVNSGVPATVEHRAAAAASATTSAAIVAECVQN 120
           STLKDTVPSIERFADTYKMDCPAAINRLV SGVPATVEHRA+AAAS+TTSAAIVAECVQN
Sbjct: 61  STLKDTVPSIERFADTYKMDCPAAINRLVISGVPATVEHRASAAASSTTSAAIVAECVQN 120

Query: 121 FITAMDSLKLNMVAVDQVHPLLSDLSASLNKLTILPPDFEGKTKMREWLGRLSKMGAADE 180
           FITAMDSLKLNMVAVDQVHPLLSDL ASLNKLTILPPDFEGKTKM+EWLGRLSKMGAADE
Sbjct: 121 FITAMDSLKLNMVAVDQVHPLLSDLFASLNKLTILPPDFEGKTKMKEWLGRLSKMGAADE 180

Query: 181 LTEQQARQLHFDLESSYNSFMAALPTAGCHFHIHGFEECFIQ 223
           LTEQQARQLHFDLESSYNSFMAALPTA       G EE ++Q
Sbjct: 181 LTEQQARQLHFDLESSYNSFMAALPTA------DGLEELYLQ 216

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BLAST of Spg011623 vs. NCBI nr
Match: XP_022950166.1 (vacuolar protein sorting-associated protein 28 homolog 2 [Cucurbita moschata] >XP_022950167.1 vacuolar protein sorting-associated protein 28 homolog 2 [Cucurbita moschata] >XP_023544383.1 vacuolar protein sorting-associated protein 28 homolog 2 isoform X3 [Cucurbita pepo subsp. pepo] >XP_023544384.1 vacuolar protein sorting-associated protein 28 homolog 2 isoform X3 [Cucurbita pepo subsp. pepo] >KAG7033897.1 Vacuolar protein sorting-associated protein 28-like 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 394.4 bits (1012), Expect = 8.8e-106
Identity = 202/208 (97.12%), Postives = 206/208 (99.04%), Query Frame = 0


Query: 1   MEVKLWNDKREREMYDNFAELYAIIKATEKLEKAYIRDIIPSSDYETECFKLIAHFKTLA 60
           MEVKLWNDKREREMYDNFAELYAIIKATEKLEKAY+RDIIPSSDYETECFKLIAHFKTLA
Sbjct: 1   MEVKLWNDKREREMYDNFAELYAIIKATEKLEKAYVRDIIPSSDYETECFKLIAHFKTLA 60

Query: 61  STLKDTVPSIERFADTYKMDCPAAINRLVNSGVPATVEHRAAAAASATTSAAIVAECVQN 120
           STLKDTVPSIERFADTYKMDCPAAINRLV SGVPATVEHRA+AAAS+TTSAAIVAECVQN
Sbjct: 61  STLKDTVPSIERFADTYKMDCPAAINRLVISGVPATVEHRASAAASSTTSAAIVAECVQN 120

Query: 121 FITAMDSLKLNMVAVDQVHPLLSDLSASLNKLTILPPDFEGKTKMREWLGRLSKMGAADE 180
           FITAMDSLKLNMVAVDQVHPLLSDL ASLNKLTILPPDFEGKTKM+EWLGRLSKMGAADE
Sbjct: 121 FITAMDSLKLNMVAVDQVHPLLSDLFASLNKLTILPPDFEGKTKMKEWLGRLSKMGAADE 180

Query: 181 LTEQQARQLHFDLESSYNSFMAALPTAG 209
           LTEQQARQLHFDLESSYNSFMAALPTAG
Sbjct: 181 LTEQQARQLHFDLESSYNSFMAALPTAG 208

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BLAST of Spg011623 vs. ExPASy Swiss-Prot
Match: Q9S9T7 (Vacuolar protein sorting-associated protein 28 homolog 2 OS=Arabidopsis thaliana OX=3702 GN=VPS28-2 PE=1 SV=2)

HSP 1 Score: 354.8 bits (909), Expect = 1.0e-96
Identity = 179/208 (86.06%), Postives = 196/208 (94.23%), Query Frame = 0


Query: 1   MEVKLWNDKREREMYDNFAELYAIIKATEKLEKAYIRDIIPSSDYETECFKLIAHFKTLA 60
           MEVKLWNDKREREMY+NFAEL+AIIKATEKLEKAYIRD+I  S+YE+EC KLI HFKTL+
Sbjct: 2   MEVKLWNDKREREMYENFAELFAIIKATEKLEKAYIRDLINPSEYESECQKLIVHFKTLS 61

Query: 61  STLKDTVPSIERFADTYKMDCPAAINRLVNSGVPATVEHRAAAAASATTSAAIVAECVQN 120
           +TLKDTVP+IERFADTYKMDCPAA+ RLV SG+PATVEHRA  AAS + SA+IVAECVQN
Sbjct: 62  ATLKDTVPNIERFADTYKMDCPAALYRLVTSGLPATVEHRATVAASTSNSASIVAECVQN 121

Query: 121 FITAMDSLKLNMVAVDQVHPLLSDLSASLNKLTILPPDFEGKTKMREWLGRLSKMGAADE 180
           FIT+MDSLKLNMVAVDQV+PLLSDLSASLNKL+ILPPDFEGKTKM+EWL RLSKMGAADE
Sbjct: 122 FITSMDSLKLNMVAVDQVYPLLSDLSASLNKLSILPPDFEGKTKMKEWLSRLSKMGAADE 181

Query: 181 LTEQQARQLHFDLESSYNSFMAALPTAG 209
           LTEQQ+RQLHFDLESSYNSFMAALP AG
Sbjct: 182 LTEQQSRQLHFDLESSYNSFMAALPKAG 209

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BLAST of Spg011623 vs. ExPASy Swiss-Prot
Match: O65421 (Vacuolar protein sorting-associated protein 28 homolog 1 OS=Arabidopsis thaliana OX=3702 GN=VPS28-1 PE=1 SV=1)

HSP 1 Score: 349.7 bits (896), Expect = 3.3e-95
Identity = 177/208 (85.10%), Postives = 196/208 (94.23%), Query Frame = 0


Query: 1   MEVKLWNDKREREMYDNFAELYAIIKATEKLEKAYIRDIIPSSDYETECFKLIAHFKTLA 60
           MEVKLWNDKREREMY+NFAELYAIIKATEKLEKAYIRD+I  S+YETEC KLI HFKTL+
Sbjct: 1   MEVKLWNDKREREMYENFAELYAIIKATEKLEKAYIRDLISPSEYETECQKLIVHFKTLS 60

Query: 61  STLKDTVPSIERFADTYKMDCPAAINRLVNSGVPATVEHRAAAAASATTSAAIVAECVQN 120
           ++LKD VP+IERFA+TYKMDC AA+ RLV SGVPATVEHRAAA+AS ++SA++VAECVQN
Sbjct: 61  ASLKDMVPNIERFAETYKMDCSAAVYRLVTSGVPATVEHRAAASASTSSSASVVAECVQN 120

Query: 121 FITAMDSLKLNMVAVDQVHPLLSDLSASLNKLTILPPDFEGKTKMREWLGRLSKMGAADE 180
           FIT+MDSLKLNMVAVDQV+PLLSDLSASLNKL+ILPPDFEGK KM+EWL RLSKMGA+DE
Sbjct: 121 FITSMDSLKLNMVAVDQVYPLLSDLSASLNKLSILPPDFEGKIKMKEWLLRLSKMGASDE 180

Query: 181 LTEQQARQLHFDLESSYNSFMAALPTAG 209
           LTEQQARQLHFDLESSYNSFMAALP AG
Sbjct: 181 LTEQQARQLHFDLESSYNSFMAALPNAG 208

[Close]

BLAST of Spg011623 vs. ExPASy Swiss-Prot
Match: Q54NF1 (Vacuolar protein sorting-associated protein 28 OS=Dictyostelium discoideum OX=44689 GN=vps28 PE=3 SV=1)

HSP 1 Score: 201.8 bits (512), Expect = 1.1e-50
Identity = 107/203 (52.71%), Postives = 142/203 (69.95%), Query Frame = 0


Query: 2   EVKLWNDKREREMYDNFAELYAIIKATEKLEKAYIRDIIPSSDYETECFKLIAHFKTLAS 61
           EVKL+N+  EREMY+N AELY+IIK TE LEKAYIRD +   DY T C KLIA FK+  +
Sbjct: 84  EVKLFNNNIEREMYENLAELYSIIKVTEHLEKAYIRDDVSPKDYTTACSKLIAQFKSSQT 143

Query: 62  TLKDTVPSIERFADTYKMDCPAAINRLVNSGVPATVEHRAAAAASATTSAAIVAECVQNF 121
            LKD V ++ +F   Y ++C AA +RLV  G P+T+EH    +++ +  A  VAE VQ F
Sbjct: 144 LLKDQVSNVGQFMKDYDLNCKAAFDRLVIKGFPSTLEHNTNESSTDSAMAKNVAEAVQLF 203

Query: 122 ITAMDSLKLNMVAVDQVHPLLSDLSASLNKLTILPPDFEGKTKMREWLGRLSKMGAADEL 181
           IT MDS++L +V+VD ++PLLSDL  SLNK   L P FEGK K++ W+  L++M A DEL
Sbjct: 204 ITTMDSIRLKLVSVDGIYPLLSDLMESLNKNQWLGPTFEGKEKIKNWISILNQMKATDEL 263

Query: 182 TEQQARQLHFDLESSYNSFMAAL 205
            + Q+RQL FDL++SYN F  A+
Sbjct: 264 DDDQSRQLLFDLDNSYNIFYKAI 286

[Close]

BLAST of Spg011623 vs. ExPASy Swiss-Prot
Match: Q9V359 (Vacuolar protein sorting-associated protein 28 homolog OS=Drosophila melanogaster OX=7227 GN=Vps28 PE=1 SV=1)

HSP 1 Score: 157.5 bits (397), Expect = 2.4e-37
Identity = 84/200 (42.00%), Postives = 124/200 (62.00%), Query Frame = 0


Query: 2   EVKLWNDKREREMYDNFAELYAIIKATEKLEKAYIRDIIPSSDYETECFKLIAHFKTLAS 61
           EVKL+ + RERE YDN A+LYAII   ++LEKAYIRD I   +Y   C K +  +K    
Sbjct: 11  EVKLFRNAREREKYDNMADLYAIINTIQQLEKAYIRDCITPQEYTAACSKYLVQYKVAFK 70

Query: 62  TLK-DTVPSIERFADTYKMDCPAAINRLVNSGVPATVEHRAAAAASATTSAAIVAECVQN 121
            ++ D  PS+E F   +++DCPAA+ R +    P T+            ++  +AE V  
Sbjct: 71  QVQCDEFPSVETFVKKFRLDCPAALER-IREDRPITIRD------DKGNTSKCIAEIVSL 130

Query: 122 FITAMDSLKLNMVAVDQVHPLLSDLSASLNKLTILPPDFEGKTKMREWLGRLSKMGAADE 181
           FIT MD L+L +  +D + P + DL+ ++N+L+++P DF+ K K+ +WLG L++M A+DE
Sbjct: 131 FITIMDKLRLQINTMDALQPDVKDLADNMNRLSLIPEDFDAKLKVEKWLGSLNEMQASDE 190

Query: 182 LTEQQARQLHFDLESSYNSF 201
           L+E Q RQ  FDLES+Y  F
Sbjct: 191 LSEGQVRQFLFDLESAYADF 203

[Close]

BLAST of Spg011623 vs. ExPASy Swiss-Prot
Match: Q9UK41 (Vacuolar protein sorting-associated protein 28 homolog OS=Homo sapiens OX=9606 GN=VPS28 PE=1 SV=1)

HSP 1 Score: 151.4 bits (381), Expect = 1.7e-35
Identity = 79/200 (39.50%), Postives = 125/200 (62.50%), Query Frame = 0


Query: 2   EVKLWNDKREREMYDNFAELYAIIKATEKLEKAYIRDIIPSSDYETECFKLIAHFKTLAS 61
           EVKL+ + RERE YDN AEL+A++K  + LEKAYI+D +  S+Y   C +L+  +K    
Sbjct: 23  EVKLYKNAREREKYDNMAELFAVVKTMQALEKAYIKDCVSPSEYTAACSRLLVQYKAAFR 82

Query: 62  TLKDT-VPSIERFADTYKMDCPAAINRLVNSGVPATVEHRAAAAASATTSAAIVAECVQN 121
            ++ + + SI+ F   +++DCP A+ R +    P T++               +A+ V  
Sbjct: 83  QVQGSEISSIDEFCRKFRLDCPLAMER-IKEDRPITIKD------DKGNLNRCIADVVSL 142

Query: 122 FITAMDSLKLNMVAVDQVHPLLSDLSASLNKLTILPPDFEGKTKMREWLGRLSKMGAADE 181
           FIT MD L+L + A+D++ P L +L  ++++++ LPPDFEG+  + +WL  LS M A+DE
Sbjct: 143 FITVMDKLRLEIRAMDEIQPDLRELMETMHRMSHLPPDFEGRQTVSQWLQTLSGMSASDE 202

Query: 182 LTEQQARQLHFDLESSYNSF 201
           L + Q RQ+ FDLES+YN+F
Sbjct: 203 LDDSQVRQMLFDLESAYNAF 215

[Close]

BLAST of Spg011623 vs. ExPASy TrEMBL
Match: A0A6J1CL71 (Vacuolar protein sorting-associated protein 28 homolog OS=Momordica charantia OX=3673 GN=LOC111012452 PE=3 SV=1)

HSP 1 Score: 401.0 bits (1029), Expect = 4.6e-108
Identity = 207/208 (99.52%), Postives = 207/208 (99.52%), Query Frame = 0


Query: 1   MEVKLWNDKREREMYDNFAELYAIIKATEKLEKAYIRDIIPSSDYETECFKLIAHFKTLA 60
           MEVKLWNDKREREMYDNFAELYAIIKATEKLEKAYIRDIIPSSDYETECFKLIAHFKTLA
Sbjct: 1   MEVKLWNDKREREMYDNFAELYAIIKATEKLEKAYIRDIIPSSDYETECFKLIAHFKTLA 60

Query: 61  STLKDTVPSIERFADTYKMDCPAAINRLVNSGVPATVEHRAAAAASATTSAAIVAECVQN 120
           STLKDTVPSIERFADTYKMDCPAAINRLVNSGVPATVEHRAAAAASATTSAAIVAECVQN
Sbjct: 61  STLKDTVPSIERFADTYKMDCPAAINRLVNSGVPATVEHRAAAAASATTSAAIVAECVQN 120

Query: 121 FITAMDSLKLNMVAVDQVHPLLSDLSASLNKLTILPPDFEGKTKMREWLGRLSKMGAADE 180
           FITAMDSLKLNMVAVDQVHPLLSDLSASLNKLTILP DFEGKTKMREWLGRLSKMGAADE
Sbjct: 121 FITAMDSLKLNMVAVDQVHPLLSDLSASLNKLTILPADFEGKTKMREWLGRLSKMGAADE 180

Query: 181 LTEQQARQLHFDLESSYNSFMAALPTAG 209
           LTEQQARQLHFDLESSYNSFMAALPTAG
Sbjct: 181 LTEQQARQLHFDLESSYNSFMAALPTAG 208

[Close]

BLAST of Spg011623 vs. ExPASy TrEMBL
Match: A0A6J1GE20 (Vacuolar protein sorting-associated protein 28 homolog OS=Cucurbita moschata OX=3662 GN=LOC111453341 PE=3 SV=1)

HSP 1 Score: 394.4 bits (1012), Expect = 4.3e-106
Identity = 202/208 (97.12%), Postives = 206/208 (99.04%), Query Frame = 0


Query: 1   MEVKLWNDKREREMYDNFAELYAIIKATEKLEKAYIRDIIPSSDYETECFKLIAHFKTLA 60
           MEVKLWNDKREREMYDNFAELYAIIKATEKLEKAY+RDIIPSSDYETECFKLIAHFKTLA
Sbjct: 1   MEVKLWNDKREREMYDNFAELYAIIKATEKLEKAYVRDIIPSSDYETECFKLIAHFKTLA 60

Query: 61  STLKDTVPSIERFADTYKMDCPAAINRLVNSGVPATVEHRAAAAASATTSAAIVAECVQN 120
           STLKDTVPSIERFADTYKMDCPAAINRLV SGVPATVEHRA+AAAS+TTSAAIVAECVQN
Sbjct: 61  STLKDTVPSIERFADTYKMDCPAAINRLVISGVPATVEHRASAAASSTTSAAIVAECVQN 120

Query: 121 FITAMDSLKLNMVAVDQVHPLLSDLSASLNKLTILPPDFEGKTKMREWLGRLSKMGAADE 180
           FITAMDSLKLNMVAVDQVHPLLSDL ASLNKLTILPPDFEGKTKM+EWLGRLSKMGAADE
Sbjct: 121 FITAMDSLKLNMVAVDQVHPLLSDLFASLNKLTILPPDFEGKTKMKEWLGRLSKMGAADE 180

Query: 181 LTEQQARQLHFDLESSYNSFMAALPTAG 209
           LTEQQARQLHFDLESSYNSFMAALPTAG
Sbjct: 181 LTEQQARQLHFDLESSYNSFMAALPTAG 208

[Close]

BLAST of Spg011623 vs. ExPASy TrEMBL
Match: A0A1S3B0K8 (Vacuolar protein sorting-associated protein 28 homolog OS=Cucumis melo OX=3656 GN=LOC103484547 PE=3 SV=1)

HSP 1 Score: 394.0 bits (1011), Expect = 5.6e-106
Identity = 203/208 (97.60%), Postives = 206/208 (99.04%), Query Frame = 0


Query: 1   MEVKLWNDKREREMYDNFAELYAIIKATEKLEKAYIRDIIPSSDYETECFKLIAHFKTLA 60
           MEVKLWNDKREREMYDNFAELYAIIKATEKLEKAYIRDII SSDYETECFKLIAHFKTLA
Sbjct: 1   MEVKLWNDKREREMYDNFAELYAIIKATEKLEKAYIRDIISSSDYETECFKLIAHFKTLA 60

Query: 61  STLKDTVPSIERFADTYKMDCPAAINRLVNSGVPATVEHRAAAAASATTSAAIVAECVQN 120
           STLKDTVPSIERFADTYKMDCPAAINRLV +GVPATVEHRAAAAASATTSAAIVAECVQN
Sbjct: 61  STLKDTVPSIERFADTYKMDCPAAINRLVITGVPATVEHRAAAAASATTSAAIVAECVQN 120

Query: 121 FITAMDSLKLNMVAVDQVHPLLSDLSASLNKLTILPPDFEGKTKMREWLGRLSKMGAADE 180
           FITAMDSLKLNMVAVDQVHPLLSDL+ASLNKLTILPPDFEGKTKM+EWLGRLSKMGAADE
Sbjct: 121 FITAMDSLKLNMVAVDQVHPLLSDLAASLNKLTILPPDFEGKTKMKEWLGRLSKMGAADE 180

Query: 181 LTEQQARQLHFDLESSYNSFMAALPTAG 209
           LTEQQARQLHFDLESSYNSFMAALPTAG
Sbjct: 181 LTEQQARQLHFDLESSYNSFMAALPTAG 208

[Close]

BLAST of Spg011623 vs. ExPASy TrEMBL
Match: A0A5D3CMS3 (Vacuolar protein sorting-associated protein 28 homolog OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G007210 PE=3 SV=1)

HSP 1 Score: 394.0 bits (1011), Expect = 5.6e-106
Identity = 203/208 (97.60%), Postives = 206/208 (99.04%), Query Frame = 0


Query: 1   MEVKLWNDKREREMYDNFAELYAIIKATEKLEKAYIRDIIPSSDYETECFKLIAHFKTLA 60
           MEVKLWNDKREREMYDNFAELYAIIKATEKLEKAYIRDII SSDYETECFKLIAHFKTLA
Sbjct: 1   MEVKLWNDKREREMYDNFAELYAIIKATEKLEKAYIRDIISSSDYETECFKLIAHFKTLA 60

Query: 61  STLKDTVPSIERFADTYKMDCPAAINRLVNSGVPATVEHRAAAAASATTSAAIVAECVQN 120
           STLKDTVPSIERFADTYKMDCPAAINRLV +GVPATVEHRAAAAASATTSAAIVAECVQN
Sbjct: 61  STLKDTVPSIERFADTYKMDCPAAINRLVITGVPATVEHRAAAAASATTSAAIVAECVQN 120

Query: 121 FITAMDSLKLNMVAVDQVHPLLSDLSASLNKLTILPPDFEGKTKMREWLGRLSKMGAADE 180
           FITAMDSLKLNMVAVDQVHPLLSDL+ASLNKLTILPPDFEGKTKM+EWLGRLSKMGAADE
Sbjct: 121 FITAMDSLKLNMVAVDQVHPLLSDLAASLNKLTILPPDFEGKTKMKEWLGRLSKMGAADE 180

Query: 181 LTEQQARQLHFDLESSYNSFMAALPTAG 209
           LTEQQARQLHFDLESSYNSFMAALPTAG
Sbjct: 181 LTEQQARQLHFDLESSYNSFMAALPTAG 208

[Close]

BLAST of Spg011623 vs. ExPASy TrEMBL
Match: A0A0A0KI07 (Vacuolar protein sorting-associated protein 28 homolog OS=Cucumis sativus OX=3659 GN=Csa_6G517060 PE=3 SV=1)

HSP 1 Score: 393.3 bits (1009), Expect = 9.5e-106
Identity = 203/208 (97.60%), Postives = 205/208 (98.56%), Query Frame = 0


Query: 1   MEVKLWNDKREREMYDNFAELYAIIKATEKLEKAYIRDIIPSSDYETECFKLIAHFKTLA 60
           MEVKLWNDKREREMYDNFAELYAIIKATEKLEKAYIRDII SSDYE ECFKLIAHFKTLA
Sbjct: 1   MEVKLWNDKREREMYDNFAELYAIIKATEKLEKAYIRDIISSSDYEAECFKLIAHFKTLA 60

Query: 61  STLKDTVPSIERFADTYKMDCPAAINRLVNSGVPATVEHRAAAAASATTSAAIVAECVQN 120
           STLKDTVPSIERFADTYKMDCPAAINRLV +GVPATVEHRAAAAASATTSAAIVAECVQN
Sbjct: 61  STLKDTVPSIERFADTYKMDCPAAINRLVITGVPATVEHRAAAAASATTSAAIVAECVQN 120

Query: 121 FITAMDSLKLNMVAVDQVHPLLSDLSASLNKLTILPPDFEGKTKMREWLGRLSKMGAADE 180
           FITAMDSLKLNMVAVDQVHPLLSDLSASLNKLTILPPDFEGKTKM+EWLGRLSKMGAADE
Sbjct: 121 FITAMDSLKLNMVAVDQVHPLLSDLSASLNKLTILPPDFEGKTKMKEWLGRLSKMGAADE 180

Query: 181 LTEQQARQLHFDLESSYNSFMAALPTAG 209
           LTEQQARQLHFDLESSYNSFMAALPTAG
Sbjct: 181 LTEQQARQLHFDLESSYNSFMAALPTAG 208

[Close]

BLAST of Spg011623 vs. TAIR 10
Match: AT4G05000.1 (Vacuolar protein sorting-associated protein VPS28 family protein )

HSP 1 Score: 354.8 bits (909), Expect = 7.2e-98
Identity = 179/208 (86.06%), Postives = 196/208 (94.23%), Query Frame = 0


Query: 1   MEVKLWNDKREREMYDNFAELYAIIKATEKLEKAYIRDIIPSSDYETECFKLIAHFKTLA 60
           MEVKLWNDKREREMY+NFAEL+AIIKATEKLEKAYIRD+I  S+YE+EC KLI HFKTL+
Sbjct: 2   MEVKLWNDKREREMYENFAELFAIIKATEKLEKAYIRDLINPSEYESECQKLIVHFKTLS 61

Query: 61  STLKDTVPSIERFADTYKMDCPAAINRLVNSGVPATVEHRAAAAASATTSAAIVAECVQN 120
           +TLKDTVP+IERFADTYKMDCPAA+ RLV SG+PATVEHRA  AAS + SA+IVAECVQN
Sbjct: 62  ATLKDTVPNIERFADTYKMDCPAALYRLVTSGLPATVEHRATVAASTSNSASIVAECVQN 121

Query: 121 FITAMDSLKLNMVAVDQVHPLLSDLSASLNKLTILPPDFEGKTKMREWLGRLSKMGAADE 180
           FIT+MDSLKLNMVAVDQV+PLLSDLSASLNKL+ILPPDFEGKTKM+EWL RLSKMGAADE
Sbjct: 122 FITSMDSLKLNMVAVDQVYPLLSDLSASLNKLSILPPDFEGKTKMKEWLSRLSKMGAADE 181

Query: 181 LTEQQARQLHFDLESSYNSFMAALPTAG 209
           LTEQQ+RQLHFDLESSYNSFMAALP AG
Sbjct: 182 LTEQQSRQLHFDLESSYNSFMAALPKAG 209

[Close]

BLAST of Spg011623 vs. TAIR 10
Match: AT4G05000.2 (Vacuolar protein sorting-associated protein VPS28 family protein )

HSP 1 Score: 354.8 bits (909), Expect = 7.2e-98
Identity = 179/208 (86.06%), Postives = 196/208 (94.23%), Query Frame = 0


Query: 1   MEVKLWNDKREREMYDNFAELYAIIKATEKLEKAYIRDIIPSSDYETECFKLIAHFKTLA 60
           MEVKLWNDKREREMY+NFAEL+AIIKATEKLEKAYIRD+I  S+YE+EC KLI HFKTL+
Sbjct: 2   MEVKLWNDKREREMYENFAELFAIIKATEKLEKAYIRDLINPSEYESECQKLIVHFKTLS 61

Query: 61  STLKDTVPSIERFADTYKMDCPAAINRLVNSGVPATVEHRAAAAASATTSAAIVAECVQN 120
           +TLKDTVP+IERFADTYKMDCPAA+ RLV SG+PATVEHRA  AAS + SA+IVAECVQN
Sbjct: 62  ATLKDTVPNIERFADTYKMDCPAALYRLVTSGLPATVEHRATVAASTSNSASIVAECVQN 121

Query: 121 FITAMDSLKLNMVAVDQVHPLLSDLSASLNKLTILPPDFEGKTKMREWLGRLSKMGAADE 180
           FIT+MDSLKLNMVAVDQV+PLLSDLSASLNKL+ILPPDFEGKTKM+EWL RLSKMGAADE
Sbjct: 122 FITSMDSLKLNMVAVDQVYPLLSDLSASLNKLSILPPDFEGKTKMKEWLSRLSKMGAADE 181

Query: 181 LTEQQARQLHFDLESSYNSFMAALPTAG 209
           LTEQQ+RQLHFDLESSYNSFMAALP AG
Sbjct: 182 LTEQQSRQLHFDLESSYNSFMAALPKAG 209

[Close]

BLAST of Spg011623 vs. TAIR 10
Match: AT4G21560.1 (vacuolar protein sorting-associated protein 28 homolog 1 )

HSP 1 Score: 349.7 bits (896), Expect = 2.3e-96
Identity = 177/208 (85.10%), Postives = 196/208 (94.23%), Query Frame = 0


Query: 1   MEVKLWNDKREREMYDNFAELYAIIKATEKLEKAYIRDIIPSSDYETECFKLIAHFKTLA 60
           MEVKLWNDKREREMY+NFAELYAIIKATEKLEKAYIRD+I  S+YETEC KLI HFKTL+
Sbjct: 1   MEVKLWNDKREREMYENFAELYAIIKATEKLEKAYIRDLISPSEYETECQKLIVHFKTLS 60

Query: 61  STLKDTVPSIERFADTYKMDCPAAINRLVNSGVPATVEHRAAAAASATTSAAIVAECVQN 120
           ++LKD VP+IERFA+TYKMDC AA+ RLV SGVPATVEHRAAA+AS ++SA++VAECVQN
Sbjct: 61  ASLKDMVPNIERFAETYKMDCSAAVYRLVTSGVPATVEHRAAASASTSSSASVVAECVQN 120

Query: 121 FITAMDSLKLNMVAVDQVHPLLSDLSASLNKLTILPPDFEGKTKMREWLGRLSKMGAADE 180
           FIT+MDSLKLNMVAVDQV+PLLSDLSASLNKL+ILPPDFEGK KM+EWL RLSKMGA+DE
Sbjct: 121 FITSMDSLKLNMVAVDQVYPLLSDLSASLNKLSILPPDFEGKIKMKEWLLRLSKMGASDE 180

Query: 181 LTEQQARQLHFDLESSYNSFMAALPTAG 209
           LTEQQARQLHFDLESSYNSFMAALP AG
Sbjct: 181 LTEQQARQLHFDLESSYNSFMAALPNAG 208

[Close]

BLAST of Spg011623 vs. TAIR 10
Match: AT4G21560.2 (vacuolar protein sorting-associated protein 28 homolog 1 )

HSP 1 Score: 349.7 bits (896), Expect = 2.3e-96
Identity = 177/208 (85.10%), Postives = 196/208 (94.23%), Query Frame = 0


Query: 1   MEVKLWNDKREREMYDNFAELYAIIKATEKLEKAYIRDIIPSSDYETECFKLIAHFKTLA 60
           MEVKLWNDKREREMY+NFAELYAIIKATEKLEKAYIRD+I  S+YETEC KLI HFKTL+
Sbjct: 1   MEVKLWNDKREREMYENFAELYAIIKATEKLEKAYIRDLISPSEYETECQKLIVHFKTLS 60

Query: 61  STLKDTVPSIERFADTYKMDCPAAINRLVNSGVPATVEHRAAAAASATTSAAIVAECVQN 120
           ++LKD VP+IERFA+TYKMDC AA+ RLV SGVPATVEHRAAA+AS ++SA++VAECVQN
Sbjct: 61  ASLKDMVPNIERFAETYKMDCSAAVYRLVTSGVPATVEHRAAASASTSSSASVVAECVQN 120

Query: 121 FITAMDSLKLNMVAVDQVHPLLSDLSASLNKLTILPPDFEGKTKMREWLGRLSKMGAADE 180
           FIT+MDSLKLNMVAVDQV+PLLSDLSASLNKL+ILPPDFEGK KM+EWL RLSKMGA+DE
Sbjct: 121 FITSMDSLKLNMVAVDQVYPLLSDLSASLNKLSILPPDFEGKIKMKEWLLRLSKMGASDE 180

Query: 181 LTEQQARQLHFDLESSYNSFMAALPTAG 209
           LTEQQARQLHFDLESSYNSFMAALP AG
Sbjct: 181 LTEQQARQLHFDLESSYNSFMAALPNAG 208

[Close]

BLAST of Spg011623 vs. TAIR 10
Match: AT4G21560.3 (vacuolar protein sorting-associated protein 28 homolog 1 )

HSP 1 Score: 349.7 bits (896), Expect = 2.3e-96
Identity = 177/208 (85.10%), Postives = 196/208 (94.23%), Query Frame = 0


Query: 1   MEVKLWNDKREREMYDNFAELYAIIKATEKLEKAYIRDIIPSSDYETECFKLIAHFKTLA 60
           MEVKLWNDKREREMY+NFAELYAIIKATEKLEKAYIRD+I  S+YETEC KLI HFKTL+
Sbjct: 1   MEVKLWNDKREREMYENFAELYAIIKATEKLEKAYIRDLISPSEYETECQKLIVHFKTLS 60

Query: 61  STLKDTVPSIERFADTYKMDCPAAINRLVNSGVPATVEHRAAAAASATTSAAIVAECVQN 120
           ++LKD VP+IERFA+TYKMDC AA+ RLV SGVPATVEHRAAA+AS ++SA++VAECVQN
Sbjct: 61  ASLKDMVPNIERFAETYKMDCSAAVYRLVTSGVPATVEHRAAASASTSSSASVVAECVQN 120

Query: 121 FITAMDSLKLNMVAVDQVHPLLSDLSASLNKLTILPPDFEGKTKMREWLGRLSKMGAADE 180
           FIT+MDSLKLNMVAVDQV+PLLSDLSASLNKL+ILPPDFEGK KM+EWL RLSKMGA+DE
Sbjct: 121 FITSMDSLKLNMVAVDQVYPLLSDLSASLNKLSILPPDFEGKIKMKEWLLRLSKMGASDE 180

Query: 181 LTEQQARQLHFDLESSYNSFMAALPTAG 209
           LTEQQARQLHFDLESSYNSFMAALP AG
Sbjct: 181 LTEQQARQLHFDLESSYNSFMAALPNAG 208

The following BLAST results are available for this feature:

BLAST of Spg011623 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022142298.1	9.4e-108	99.52	vacuolar protein sorting-associated protein 28 homolog 2 [Momordica charantia] >...	[more]
XP_038883329.1	5.2e-106	98.08	vacuolar protein sorting-associated protein 28 homolog 2 [Benincasa hispida] >XP...	[more]
XP_023544381.1	8.8e-106	97.12	vacuolar protein sorting-associated protein 28 homolog 2 isoform X1 [Cucurbita p...	[more]
KAG6603719.1	8.8e-106	92.34	Vacuolar protein sorting-associated protein 28-like 2, partial [Cucurbita argyro...	[more]
XP_022950166.1	8.8e-106	97.12	vacuolar protein sorting-associated protein 28 homolog 2 [Cucurbita moschata] >X...	[more]

BLAST of Spg011623 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9S9T7	1.0e-96	86.06	Vacuolar protein sorting-associated protein 28 homolog 2 OS=Arabidopsis thaliana...	[more]
O65421	3.3e-95	85.10	Vacuolar protein sorting-associated protein 28 homolog 1 OS=Arabidopsis thaliana...	[more]
Q54NF1	1.1e-50	52.71	Vacuolar protein sorting-associated protein 28 OS=Dictyostelium discoideum OX=44...	[more]
Q9V359	2.4e-37	42.00	Vacuolar protein sorting-associated protein 28 homolog OS=Drosophila melanogaste...	[more]
Q9UK41	1.7e-35	39.50	Vacuolar protein sorting-associated protein 28 homolog OS=Homo sapiens OX=9606 G...	[more]

BLAST of Spg011623 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CL71	4.6e-108	99.52	Vacuolar protein sorting-associated protein 28 homolog OS=Momordica charantia OX...	[more]
A0A6J1GE20	4.3e-106	97.12	Vacuolar protein sorting-associated protein 28 homolog OS=Cucurbita moschata OX=...	[more]
A0A1S3B0K8	5.6e-106	97.60	Vacuolar protein sorting-associated protein 28 homolog OS=Cucumis melo OX=3656 G...	[more]
A0A5D3CMS3	5.6e-106	97.60	Vacuolar protein sorting-associated protein 28 homolog OS=Cucumis melo var. maku...	[more]
A0A0A0KI07	9.5e-106	97.60	Vacuolar protein sorting-associated protein 28 homolog OS=Cucumis sativus OX=365...	[more]

BLAST of Spg011623 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G05000.1	7.2e-98	86.06	Vacuolar protein sorting-associated protein VPS28 family protein	[more]
AT4G05000.2	7.2e-98	86.06	Vacuolar protein sorting-associated protein VPS28 family protein	[more]
AT4G21560.1	2.3e-96	85.10	vacuolar protein sorting-associated protein 28 homolog 1	[more]
AT4G21560.2	2.3e-96	85.10	vacuolar protein sorting-associated protein 28 homolog 1	[more]
AT4G21560.3	2.3e-96	85.10	vacuolar protein sorting-associated protein 28 homolog 1	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR037206	VPS28, C-terminal domain superfamily	GENE3D	1.20.120.1130		coord: 106..205 e-value: 1.9E-40 score: 138.8
IPR037206	VPS28, C-terminal domain superfamily	SUPERFAMILY	140427	VPS28 C-terminal domain-like	coord: 110..204
IPR007143	Vacuolar protein sorting-associated Vps28	PFAM	PF03997	VPS28	coord: 18..204 e-value: 2.3E-69 score: 232.9
IPR007143	Vacuolar protein sorting-associated Vps28	PANTHER	PTHR12937	VACUOLAR PROTEIN SORTING 28, ISOFORM 2 VPS28	coord: 1..208
IPR038358	VPS28, N-terminal domain superfamily	GENE3D	1.20.1440.200		coord: 1..91 e-value: 2.6E-31 score: 110.1
None	No IPR available	PANTHER	PTHR12937:SF3	VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 28 HOMOLOG 1	coord: 1..208
IPR017898	Vacuolar protein sorting-associated, VPS28, N-terminal	PROSITE	PS51313	VPS28_N	coord: 1..99 score: 31.518972
IPR017899	Vacuolar protein sorting-associated, VPS28, C-terminal	PROSITE	PS51310	VPS28_C	coord: 109..205 score: 44.760601
IPR037202	ESCRT assembly domain	SUPERFAMILY	140111	Endosomal sorting complex assembly domain	coord: 2..97

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg011623.1	Spg011623.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043328	protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway
biological_process	GO:0032509	endosome transport via multivesicular body sorting pathway
cellular_component	GO:0000813	ESCRT I complex
molecular_function	GO:0044877	protein-containing complex binding