Spg010521 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg010521 vs. NCBI nr
Match: XP_022971196.1 (cytochrome P450 94A1-like [Cucurbita maxima])

HSP 1 Score: 916.4 bits (2367), Expect = 1.1e-262
Identity = 449/508 (88.39%), Postives = 479/508 (94.29%), Query Frame = 0

Query: 1   MDISATTLFFLTFLSLSLYLLFFTSQKKSKTHQGFKHFPILGTLPLFLLNRHRFLDWSTE 60
           MDISAT   FLTFLSL LYLL FTS KK K++QGFKHFPI+GTLPLFLLNRHRFLDWSTE
Sbjct: 1   MDISATAFLFLTFLSLCLYLLLFTSPKKPKSNQGFKHFPIVGTLPLFLLNRHRFLDWSTE 60

Query: 61  VLSSCPTNTAVFKRPGKVHGVITANPLVVEHILKTQFENYPKGERFISLLEDFLGRGIFN 120
           VLS+C TNTAVF+RPGKVHGVITANPLVVEHILKTQFENYPKG+RFISLLEDFLGRGIFN
Sbjct: 61  VLSNCQTNTAVFRRPGKVHGVITANPLVVEHILKTQFENYPKGKRFISLLEDFLGRGIFN 120

Query: 121 SDGEIWRVQRKTASYEFNTKSLRNFVMENVRVEIQTRLLPILGKASETERILDLQDVLER 180
           SDGEIW+VQRKTASYEFNTKSLRNFVMENV VEIQTRLLPILGKASETERILDLQDVLER
Sbjct: 121 SDGEIWKVQRKTASYEFNTKSLRNFVMENVTVEIQTRLLPILGKASETERILDLQDVLER 180

Query: 181 FAFDNVCKLAFDFDPGCLGGDGTAGAEFMRAFEDAATLSSGRFMYAFPGLYKVKKILNIG 240
           FAFDNVCKLAF+FDP CLGGDGT+GA+FMRAFEDAATLSSGRFMYA PGLYKVKK LN+G
Sbjct: 181 FAFDNVCKLAFNFDPCCLGGDGTSGADFMRAFEDAATLSSGRFMYALPGLYKVKKFLNMG 240

Query: 241 SERTLKESIATVHKFAEDIIRSRMEEKKTTHIKNDQDLLSRFMGDEDYNSPEFLRDIIIS 300
           SERTLK+SIA VHKFAEDIIRSRMEEKKTTH+KN+QDLLSRFMG ++ NSPEFLRDIIIS
Sbjct: 241 SERTLKKSIARVHKFAEDIIRSRMEEKKTTHVKNNQDLLSRFMGTQENNSPEFLRDIIIS 300

Query: 301 FILAGRDTTSSALTWFFWILSSRPHIQQKILTELETIRSRTSKQIGETYSFDELRDMHYL 360
           FILAGRDTTSSALTWFFWILSSRP+I QKIL ELETIRSRT K++G  YSF+ELRDMHYL
Sbjct: 301 FILAGRDTTSSALTWFFWILSSRPNITQKILKELETIRSRTGKKLGAEYSFEELRDMHYL 360

Query: 361 QAALSETLRLYPPVPVDTKACRNDDVLPDGTFIGKSWFVTYHTYAMGRMESIWGKNYGEF 420
           QAALSETLRLYPPVPVDTKACRN+DVLPDGT +G+ WFVTYHTYAMGRME IWG+N GEF
Sbjct: 361 QAALSETLRLYPPVPVDTKACRNEDVLPDGTLVGRDWFVTYHTYAMGRMERIWGENCGEF 420

Query: 421 LPERWLENGVCRTESPFRFPVFHAGPRMCLGKDMAYIQMKSIAAAVMERFEVGMAEKEKS 480
           LPERW+ENGVCR ESPFRFPVFHAGPRMCLGKDMAYIQMKSIAAAV+ERFEVGMAEKEKS
Sbjct: 421 LPERWVENGVCRAESPFRFPVFHAGPRMCLGKDMAYIQMKSIAAAVIERFEVGMAEKEKS 480

Query: 481 PKHLLSLTLRMENGLQVIVKKRDTSVTI 509
           PK+LLSLTLRMENG  V++KKRD S+ I
Sbjct: 481 PKYLLSLTLRMENGCPVVLKKRDPSLAI 508

BLAST of Spg010521 vs. NCBI nr
Match: XP_022933496.1 (cytochrome P450 94A1-like [Cucurbita moschata])

HSP 1 Score: 916.0 bits (2366), Expect = 1.4e-262
Identity = 448/508 (88.19%), Postives = 479/508 (94.29%), Query Frame = 0

Query: 1   MDISATTLFFLTFLSLSLYLLFFTSQKKSKTHQGFKHFPILGTLPLFLLNRHRFLDWSTE 60
           MDISAT   FLTFLSL LYLL F S KKSK++QGFKHFPI+GTLPLFLLNRHRFLDWSTE
Sbjct: 1   MDISATAFLFLTFLSLCLYLLLFASPKKSKSNQGFKHFPIVGTLPLFLLNRHRFLDWSTE 60

Query: 61  VLSSCPTNTAVFKRPGKVHGVITANPLVVEHILKTQFENYPKGERFISLLEDFLGRGIFN 120
           VLS+C TNTAVF+RPGKVHGVITANPLVVEHILKTQFENYPKG+RFISLLEDFLGRGIFN
Sbjct: 61  VLSNCQTNTAVFRRPGKVHGVITANPLVVEHILKTQFENYPKGKRFISLLEDFLGRGIFN 120

Query: 121 SDGEIWRVQRKTASYEFNTKSLRNFVMENVRVEIQTRLLPILGKASETERILDLQDVLER 180
           SDGEIW+VQRKTASYEFNTKSLRNFVMENV VEIQTRLLPILGKASETERILDLQDVLER
Sbjct: 121 SDGEIWKVQRKTASYEFNTKSLRNFVMENVTVEIQTRLLPILGKASETERILDLQDVLER 180

Query: 181 FAFDNVCKLAFDFDPGCLGGDGTAGAEFMRAFEDAATLSSGRFMYAFPGLYKVKKILNIG 240
           FAFDNVCKLAF+FDP CLGGDGT+GA+FMRAFEDAATLSSGRFMYA PGLYKVKK LN+G
Sbjct: 181 FAFDNVCKLAFNFDPCCLGGDGTSGADFMRAFEDAATLSSGRFMYALPGLYKVKKFLNMG 240

Query: 241 SERTLKESIATVHKFAEDIIRSRMEEKKTTHIKNDQDLLSRFMGDEDYNSPEFLRDIIIS 300
           SERTLK+SIA VHKFAEDIIRSRMEEKKTTH+KNDQDLLSRFMG ++ NSPEFLRDIIIS
Sbjct: 241 SERTLKKSIARVHKFAEDIIRSRMEEKKTTHVKNDQDLLSRFMGSQENNSPEFLRDIIIS 300

Query: 301 FILAGRDTTSSALTWFFWILSSRPHIQQKILTELETIRSRTSKQIGETYSFDELRDMHYL 360
           FILAGRDTTSSALTWFFWILSSRP+I QKIL ELETIRSRT K++G  YSF+ELRDMHYL
Sbjct: 301 FILAGRDTTSSALTWFFWILSSRPNITQKILKELETIRSRTGKKLGAEYSFEELRDMHYL 360

Query: 361 QAALSETLRLYPPVPVDTKACRNDDVLPDGTFIGKSWFVTYHTYAMGRMESIWGKNYGEF 420
           QAALSETLRLYPPVPVDTKACRN+DVLPDGT +G+ WFVTYHTYAMGRME IWG+N GEF
Sbjct: 361 QAALSETLRLYPPVPVDTKACRNEDVLPDGTLVGREWFVTYHTYAMGRMERIWGENCGEF 420

Query: 421 LPERWLENGVCRTESPFRFPVFHAGPRMCLGKDMAYIQMKSIAAAVMERFEVGMAEKEKS 480
           LPERW+ENGVCR ESPFRFPVFHAGPRMCLGK+MAYIQMKSIAAAV+ERFEVGMAEKEKS
Sbjct: 421 LPERWVENGVCRAESPFRFPVFHAGPRMCLGKEMAYIQMKSIAAAVIERFEVGMAEKEKS 480

Query: 481 PKHLLSLTLRMENGLQVIVKKRDTSVTI 509
           PK+LLSLTLRMENG  V++K+RD S+ I
Sbjct: 481 PKYLLSLTLRMENGCPVVLKRRDPSLAI 508

BLAST of Spg010521 vs. NCBI nr
Match: KAG7027705.1 (Cytochrome P450 94A1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 913.7 bits (2360), Expect = 7.1e-262
Identity = 446/508 (87.80%), Postives = 479/508 (94.29%), Query Frame = 0

Query: 1   MDISATTLFFLTFLSLSLYLLFFTSQKKSKTHQGFKHFPILGTLPLFLLNRHRFLDWSTE 60
           MDISAT   FLTFLSL LYLL F S KKSK++QGFKHFPI+GTLPLFLLNRHRFLDWSTE
Sbjct: 1   MDISATAFLFLTFLSLCLYLLLFASPKKSKSNQGFKHFPIVGTLPLFLLNRHRFLDWSTE 60

Query: 61  VLSSCPTNTAVFKRPGKVHGVITANPLVVEHILKTQFENYPKGERFISLLEDFLGRGIFN 120
           VLS+C TNTAVF+RPGKVHGVITANPLVVEHILKTQFENYPKG+RFISLLEDFLGRGIFN
Sbjct: 61  VLSNCQTNTAVFRRPGKVHGVITANPLVVEHILKTQFENYPKGKRFISLLEDFLGRGIFN 120

Query: 121 SDGEIWRVQRKTASYEFNTKSLRNFVMENVRVEIQTRLLPILGKASETERILDLQDVLER 180
           SDGEIW+VQRKTASYEFNTKSLRNFVMENV VEIQTRLLPILGKASETERILDLQDVLER
Sbjct: 121 SDGEIWKVQRKTASYEFNTKSLRNFVMENVTVEIQTRLLPILGKASETERILDLQDVLER 180

Query: 181 FAFDNVCKLAFDFDPGCLGGDGTAGAEFMRAFEDAATLSSGRFMYAFPGLYKVKKILNIG 240
           FAFDNVCKLAF+FDP CLGGDGT+GA+FMRAFEDAATLSSGRFMYA PGLYKVKK LN+G
Sbjct: 181 FAFDNVCKLAFNFDPCCLGGDGTSGADFMRAFEDAATLSSGRFMYALPGLYKVKKFLNMG 240

Query: 241 SERTLKESIATVHKFAEDIIRSRMEEKKTTHIKNDQDLLSRFMGDEDYNSPEFLRDIIIS 300
           SERTLK+SIA VHKFAED+IRSRMEEKKTTH+KNDQDLLSRFMG ++ NSPEFLRDIIIS
Sbjct: 241 SERTLKKSIARVHKFAEDVIRSRMEEKKTTHVKNDQDLLSRFMGSQENNSPEFLRDIIIS 300

Query: 301 FILAGRDTTSSALTWFFWILSSRPHIQQKILTELETIRSRTSKQIGETYSFDELRDMHYL 360
           FILAGRDTTSSALTWFFWILSSRP+I QKIL ELETIRSRT K++G  YSF+ELRDMHYL
Sbjct: 301 FILAGRDTTSSALTWFFWILSSRPNITQKILKELETIRSRTGKKLGAEYSFEELRDMHYL 360

Query: 361 QAALSETLRLYPPVPVDTKACRNDDVLPDGTFIGKSWFVTYHTYAMGRMESIWGKNYGEF 420
           QAALSETLRLYPPVPVDTKACR++DVLPDGT +G+ WFVTYHTYAMGRME IWG+N GEF
Sbjct: 361 QAALSETLRLYPPVPVDTKACRHEDVLPDGTLVGREWFVTYHTYAMGRMERIWGENCGEF 420

Query: 421 LPERWLENGVCRTESPFRFPVFHAGPRMCLGKDMAYIQMKSIAAAVMERFEVGMAEKEKS 480
           LPERW+ENGVCR ESPFRFPVFHAGPRMCLGK+MAYIQMKSIAAAV+ERFEVGMAEKEKS
Sbjct: 421 LPERWVENGVCRAESPFRFPVFHAGPRMCLGKEMAYIQMKSIAAAVIERFEVGMAEKEKS 480

Query: 481 PKHLLSLTLRMENGLQVIVKKRDTSVTI 509
           PK+LLSLTLRMENG  V++K+RD S+ I
Sbjct: 481 PKYLLSLTLRMENGCPVVLKRRDPSLAI 508

BLAST of Spg010521 vs. NCBI nr
Match: XP_023538867.1 (cytochrome P450 94A1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 911.0 bits (2353), Expect = 4.6e-261
Identity = 445/508 (87.60%), Postives = 477/508 (93.90%), Query Frame = 0

Query: 1   MDISATTLFFLTFLSLSLYLLFFTSQKKSKTHQGFKHFPILGTLPLFLLNRHRFLDWSTE 60
           MDISAT   FLTFLSL LYLL F S KKSK++QGFKHFP++GTLPLFLLNRHRFLDWSTE
Sbjct: 1   MDISATAFLFLTFLSLCLYLLLFASPKKSKSNQGFKHFPLVGTLPLFLLNRHRFLDWSTE 60

Query: 61  VLSSCPTNTAVFKRPGKVHGVITANPLVVEHILKTQFENYPKGERFISLLEDFLGRGIFN 120
           VLS+C TNTAVF+RPGKVHGVITANPLVVEHILKTQFENYPKG+RFISLLEDFLGRGIFN
Sbjct: 61  VLSNCQTNTAVFRRPGKVHGVITANPLVVEHILKTQFENYPKGKRFISLLEDFLGRGIFN 120

Query: 121 SDGEIWRVQRKTASYEFNTKSLRNFVMENVRVEIQTRLLPILGKASETERILDLQDVLER 180
           SDGEIW+VQRKTASYEFNTKSLRNFVMENV VEIQTRLLPILGKASETERILDLQDVLER
Sbjct: 121 SDGEIWKVQRKTASYEFNTKSLRNFVMENVTVEIQTRLLPILGKASETERILDLQDVLER 180

Query: 181 FAFDNVCKLAFDFDPGCLGGDGTAGAEFMRAFEDAATLSSGRFMYAFPGLYKVKKILNIG 240
           FAFDNVCKLAF+FDP CLGGDGT+GA+FMRAFEDAATLSSGRFMYA PGLYKVKK  N+G
Sbjct: 181 FAFDNVCKLAFNFDPCCLGGDGTSGADFMRAFEDAATLSSGRFMYALPGLYKVKKFFNMG 240

Query: 241 SERTLKESIATVHKFAEDIIRSRMEEKKTTHIKNDQDLLSRFMGDEDYNSPEFLRDIIIS 300
           SERTLK+SIA VH+FAEDIIRSRMEEKKTTH+KNDQDLLSRFMG ++ NSPEFLRDIIIS
Sbjct: 241 SERTLKKSIARVHEFAEDIIRSRMEEKKTTHVKNDQDLLSRFMGSQENNSPEFLRDIIIS 300

Query: 301 FILAGRDTTSSALTWFFWILSSRPHIQQKILTELETIRSRTSKQIGETYSFDELRDMHYL 360
           FILAGRDTTSSALTWFFWILSSRP+I QKIL ELETIRSRT K++G  YS +ELRDMHYL
Sbjct: 301 FILAGRDTTSSALTWFFWILSSRPNITQKILKELETIRSRTGKKLGAEYSLEELRDMHYL 360

Query: 361 QAALSETLRLYPPVPVDTKACRNDDVLPDGTFIGKSWFVTYHTYAMGRMESIWGKNYGEF 420
           QAALSETLRLYPPVPVDTKACRN+DVLPDGT +G+ WFVTYHTYAMGRME IWG+N GEF
Sbjct: 361 QAALSETLRLYPPVPVDTKACRNEDVLPDGTLVGREWFVTYHTYAMGRMERIWGENCGEF 420

Query: 421 LPERWLENGVCRTESPFRFPVFHAGPRMCLGKDMAYIQMKSIAAAVMERFEVGMAEKEKS 480
           LPERW+ENGVCR ESPFRFPVFHAGPRMCLGK+MAYIQMKSIAAAV+ERFEVGMAEKEKS
Sbjct: 421 LPERWVENGVCRAESPFRFPVFHAGPRMCLGKEMAYIQMKSIAAAVIERFEVGMAEKEKS 480

Query: 481 PKHLLSLTLRMENGLQVIVKKRDTSVTI 509
           PK+LLSLTLRMENG  V++KKRD S+ I
Sbjct: 481 PKYLLSLTLRMENGCPVVLKKRDPSLAI 508

BLAST of Spg010521 vs. NCBI nr
Match: KAG6596164.1 (Cytochrome P450 94A1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 903.3 bits (2333), Expect = 9.6e-259
Identity = 441/498 (88.55%), Postives = 471/498 (94.58%), Query Frame = 0

Query: 1   MDISATTLFFLTFLSLSLYLLFFTSQKKSKTHQGFKHFPILGTLPLFLLNRHRFLDWSTE 60
           MDISAT   FLTFLSL LYLL F S KKSK++QGFKHFPI+GTLPLFLLNRHRFLDWSTE
Sbjct: 1   MDISATAFLFLTFLSLCLYLLLFASPKKSKSNQGFKHFPIVGTLPLFLLNRHRFLDWSTE 60

Query: 61  VLSSCPTNTAVFKRPGKVHGVITANPLVVEHILKTQFENYPKGERFISLLEDFLGRGIFN 120
           VLS+C TNTAVF+RPGKVHGVITANPLVVEHILKTQFENYPKG+RFISLLEDFLGRGIFN
Sbjct: 61  VLSNCQTNTAVFRRPGKVHGVITANPLVVEHILKTQFENYPKGKRFISLLEDFLGRGIFN 120

Query: 121 SDGEIWRVQRKTASYEFNTKSLRNFVMENVRVEIQTRLLPILGKASETERILDLQDVLER 180
           SDGEIW+VQRKTASYEFNTKSLRNFVMENV VEIQTRLLPILGKASETERILDLQDVLER
Sbjct: 121 SDGEIWKVQRKTASYEFNTKSLRNFVMENVTVEIQTRLLPILGKASETERILDLQDVLER 180

Query: 181 FAFDNVCKLAFDFDPGCLGGDGTAGAEFMRAFEDAATLSSGRFMYAFPGLYKVKKILNIG 240
           FAFDNVCKLAF+FDP CLGGDGT+GA+FMRAFEDAATLSSGRFMYA PGLYKVKK LN+G
Sbjct: 181 FAFDNVCKLAFNFDPCCLGGDGTSGADFMRAFEDAATLSSGRFMYALPGLYKVKKFLNMG 240

Query: 241 SERTLKESIATVHKFAEDIIRSRMEEKKTTHIKNDQDLLSRFMGDEDYNSPEFLRDIIIS 300
           SERTLK+SIA VHKFAED+IRSRMEEKKTTH+KNDQDLLSRFMG ++ NSPEFLRDIIIS
Sbjct: 241 SERTLKKSIARVHKFAEDVIRSRMEEKKTTHVKNDQDLLSRFMGSQENNSPEFLRDIIIS 300

Query: 301 FILAGRDTTSSALTWFFWILSSRPHIQQKILTELETIRSRTSKQIGETYSFDELRDMHYL 360
           FILAGRDTTSSALTWFFWILSSRP+I QKIL ELETIRSRT K++G  YSF+ELRDMHYL
Sbjct: 301 FILAGRDTTSSALTWFFWILSSRPNITQKILKELETIRSRTGKKLGAEYSFEELRDMHYL 360

Query: 361 QAALSETLRLYPPVPVDTKACRNDDVLPDGTFIGKSWFVTYHTYAMGRMESIWGKNYGEF 420
           QAALSETLRLYPPVPVDTKACR++DVLPDGT +G+ WFVTYHTYAMGRME IWG+N GEF
Sbjct: 361 QAALSETLRLYPPVPVDTKACRHEDVLPDGTLVGREWFVTYHTYAMGRMERIWGENCGEF 420

Query: 421 LPERWLENGVCRTESPFRFPVFHAGPRMCLGKDMAYIQMKSIAAAVMERFEVGMAEKEKS 480
           LPERW+ENGVCR ESPFRFPVFHAGPRMCLGK+MAYIQMKSIAAAV+ERFEVGMAEKEKS
Sbjct: 421 LPERWVENGVCRAESPFRFPVFHAGPRMCLGKEMAYIQMKSIAAAVIERFEVGMAEKEKS 480

Query: 481 PKHLLSLTLRMENGLQVI 499
           PK+LLSLTLRMENG  V+
Sbjct: 481 PKYLLSLTLRMENGCPVV 498

BLAST of Spg010521 vs. ExPASy Swiss-Prot
Match: O81117 (Cytochrome P450 94A1 OS=Vicia sativa OX=3908 GN=CYP94A1 PE=2 SV=2)

HSP 1 Score: 427.9 bits (1099), Expect = 1.6e-118
Identity = 220/478 (46.03%), Postives = 313/478 (65.48%), Query Frame = 0

Query: 36  KHFPILGTLPLFLLNRHRFLDWSTEVLSSCPTNTAVFKRPGKV--HGVITANPLVVEHIL 95
           K +P++G+   F  N HR + W ++++   P  +A F+  G +    +IT NP  V+HIL
Sbjct: 49  KSYPLIGSYLSFRKNLHRRIQWLSDIVQISP--SATFQLDGTLGKRQIITGNPSTVQHIL 108

Query: 96  KTQFENYPKGERFISLLEDFLGRGIFNSDGEIWRVQRKTASYEFNTKSLRNFVMENVRVE 155
           K QF NY KG  F + L DFLG GIFN++G  W+ QR+ AS+EFNTKS+RNFV   V  E
Sbjct: 109 KNQFSNYQKGTTFTNTLSDFLGTGIFNTNGPNWKFQRQVASHEFNTKSIRNFVEHIVDTE 168

Query: 156 IQTRLLPILGKASETERILDLQDVLERFAFDNVCKLAFDFDPGCLGGDGTAGAEFMRAFE 215
           +  RL+PIL  +++T  ILD QD+L+RF FDN+C +AF +DP  L    T  ++F  A+E
Sbjct: 169 LTNRLIPILTSSTQTNNILDFQDILQRFTFDNICNIAFGYDPEYL-TPSTNRSKFAEAYE 228

Query: 216 DAATLSSGRFMYAFPGLYKVKKILNIGSERTLKESIATVHKFAEDIIRSRMEEKKTTHIK 275
           DA  +SS RF    P ++K+KK  NIGSE+ LKE++  V  FA+ ++R +  E +     
Sbjct: 229 DATEISSKRFRLPLPIIWKIKKYFNIGSEKRLKEAVTEVRSFAKKLVREKKRELEEKSSL 288

Query: 276 NDQDLLSRFMGDEDYNSPEFLRDIIISFILAGRDTTSSALTWFFWILSSRPHIQQKILTE 335
             +D+LSRF+    ++  +F+ DI+ISFILAG+DTTS+ALTWFFW+L   P ++++I+ E
Sbjct: 289 ETEDMLSRFL-SSGHSDEDFVADIVISFILAGKDTTSAALTWFFWLLWKNPRVEEEIVNE 348

Query: 336 LETIRSRTSKQIGETYSFDELRDMHYLQAALSETLRLYPPVPVDTKACRNDDVLPDGTFI 395
           L       SK+  E   +DE+++M Y  AALSE++RLYPPVP+D+K   NDDVLPDG  +
Sbjct: 349 L-------SKK-SELMVYDEVKEMVYTHAALSESMRLYPPVPMDSKEAVNDDVLPDGWVV 408

Query: 396 GKSWFVTYHTYAMGRMESIWGKNYGEFLPERWLE----NG--VCRTESPFRFPVFHAGPR 455
            K   VTYH YAMGRM+S+WG ++ EF PERWLE    NG  V      + +PVF AGPR
Sbjct: 409 KKGTIVTYHVYAMGRMKSLWGDDWAEFRPERWLEKDEVNGKWVFVGRDSYSYPVFQAGPR 468

Query: 456 MCLGKDMAYIQMKSIAAAVMERFE-VGMAEKEKSPKHLLSLTLRMENGLQVIVKKRDT 505
           +CLGK+MA++QMK I A ++ +F+ V  A   + P  +  L+ +ME G  V ++KRD+
Sbjct: 469 VCLGKEMAFMQMKRIVAGIVGKFKVVPEAHLAQEPGFISFLSSQMEGGFPVTIQKRDS 514

BLAST of Spg010521 vs. ExPASy Swiss-Prot
Match: P98188 (Cytochrome P450 94A2 OS=Vicia sativa OX=3908 GN=CYP94A2 PE=2 SV=1)

HSP 1 Score: 419.9 bits (1078), Expect = 4.2e-116
Identity = 230/514 (44.75%), Postives = 316/514 (61.48%), Query Frame = 0

Query: 8   LFFLTFLSLSLYLLFFTSQKKSKTHQGFKHFPILGTLPLF------LLNRHRFLDWSTEV 67
           L F T L   L+L   T  K  KT     + PI  + P+F      L N HR + W++++
Sbjct: 10  LLFSTSLFWFLFLATKTKSKPPKTPSSTTNTPIPKSYPIFGSAFSLLANFHRRIQWTSDI 69

Query: 68  LSSCPTNTAVFKRPGKVHGVITANPLVVEHILKTQFENYPKGERFISLLEDFLGRGIFNS 127
           L + P++T V  RP     V TA P VV+HIL+T F  Y KG  F   + DFLG GIFN+
Sbjct: 70  LQTIPSSTFVLHRPFGARQVFTAQPAVVQHILRTNFTCYGKGLTFYQSINDFLGDGIFNA 129

Query: 128 DGEIWRVQRKTASYEFNTKSLRNFVMENVRVEIQTRLLPILGKASETERILDLQDVLERF 187
           DGE W+ QR+ +S+EFNT+SLR FV   V VE+  RL+P+L +AS ++  LD QD+L+R 
Sbjct: 130 DGESWKFQRQISSHEFNTRSLRKFVETVVDVELSDRLVPVLSQASNSQTTLDFQDILQRL 189

Query: 188 AFDNVCKLAFDFDPGCLGGDGTAGAEFMRAFEDAATLSSGRFMYAFPGLYKVKKILNIGS 247
            FDN+C +AF +DP  L         F +AF++++ LS  R     P L+KVK+ LNIG 
Sbjct: 190 TFDNICMIAFGYDPEYL-LPSLPEIPFAKAFDESSQLSIERLNALIPLLWKVKRFLNIGV 249

Query: 248 ERTLKESIATVHKFAEDIIRSRMEEKKTTHIKNDQ---DLLSRFMGDEDYNSPEFLRDII 307
           ER LKE++A V   A  I++++ +E K   ++++    DLLSRF+    ++   F+ D++
Sbjct: 250 ERQLKEAVAEVRGLATKIVKNKKKELKEKALQSESESVDLLSRFL-SSGHSDESFVTDMV 309

Query: 308 ISFILAGRDTTSSALTWFFWILSSRPHIQQKILTELETIRSRTSKQIGETYSFDELRDMH 367
           IS ILAGRDTTS+ALTWFFW+LS   H++ +IL E+      T K   ET  +DE++DM 
Sbjct: 310 ISIILAGRDTTSAALTWFFWLLSKHSHVENEILKEI------TGK--SETVGYDEVKDMV 369

Query: 368 YLQAALSETLRLYPPVPVDTKACRNDDVLPDGTFIGKSWFVTYHTYAMGRMESIWGKNYG 427
           Y  AAL E++RLYPP+PVDTK   +DDVLPDGT + K W VTYH YAMGR E IWG ++ 
Sbjct: 370 YTHAALCESMRLYPPLPVDTKVAVHDDVLPDGTLVKKGWRVTYHIYAMGRSEKIWGPDWA 429

Query: 428 EFLPERWLENGVCRTES-----PFRFPVFHAGPRMCLGKDMAYIQMKSIAAAVMERFEVG 487
           EF PERWL        S      + +PVF AGPR+C+GK+MA++QMK + A +M RF V 
Sbjct: 430 EFRPERWLSRDEVGKWSFVGIDYYSYPVFQAGPRVCIGKEMAFLQMKRVVAGIMGRFRVV 489

Query: 488 MAEKEK-SPKHLLSLTLRMENGLQVIVKKRDTSV 507
            A  E   P++    T  M+ G  V ++KR   V
Sbjct: 490 PAMVEGIEPEYTAHFTSVMKGGFPVKIEKRSPLV 513

BLAST of Spg010521 vs. ExPASy Swiss-Prot
Match: Q9SMP5 (Cytochrome P450 94B3 OS=Arabidopsis thaliana OX=3702 GN=CYP94B3 PE=1 SV=1)

HSP 1 Score: 407.9 bits (1047), Expect = 1.7e-112
Identity = 221/509 (43.42%), Postives = 324/509 (63.65%), Query Frame = 0

Query: 9   FFLTFLSLS--LYLLFFTSQKKSK-----THQGFKHFPILGTLPLFLLNRHRFLDWSTEV 68
           F L+FL L+  + ++FF S   +K     T  G   +P++G++  F  NRHR L W TE+
Sbjct: 3   FLLSFLILAFLITIIFFLSSSSTKKVQENTTYGPPSYPLIGSILSFNKNRHRLLQWYTEL 62

Query: 69  LSSCPTNTAVFKRPGKVHGVITANPLVVEHILKTQFENYPKGERFISLLEDFLGRGIFNS 128
           L   P+ T +    G    +IT NPL VE+ILKT F N+PKG+ F  LL D LG GIFN 
Sbjct: 63  LRLSPSQTILVPLLGNRRTIITTNPLNVEYILKTNFFNFPKGKPFTDLLGDLLGGGIFNV 122

Query: 129 DGEIWRVQRKTASYEFNTKSLRNFVMENVRVEIQTRLLPILGKASETERILDLQDVLERF 188
           DG  W  QRK AS+EF+T+SLR+F  E ++ E++ RL+P+L  A++    +DLQDVL+RF
Sbjct: 123 DGHSWSSQRKLASHEFSTRSLRSFAFEVLKDEVENRLVPVLSTAADVGTTVDLQDVLKRF 182

Query: 189 AFDNVCKLAFDFDPGCLGGDGTAGAE-FMRAFEDAATLSSGRFMYAFPGLYKVKKILNIG 248
           AFD VCK++  +DP CL  D T      + AF+ AA +S+ R       ++K K++LN+G
Sbjct: 183 AFDVVCKVSLGWDPDCL--DLTRPVNPLVEAFDTAAEISARRATEPIYAVWKTKRVLNVG 242

Query: 249 SERTLKESIATVHKFAEDIIRSRMEEKKT-THIKNDQDLLSRFMGDEDYNSPEFLRDIII 308
           SER L+E+I TVH    +I+R++ +  +  T  +  QDLLSRF+     ++ E +RD++I
Sbjct: 243 SERKLREAIRTVHVLVSEIVRAKKKSLEIGTGAEAKQDLLSRFLAAG--HNGEAVRDMVI 302

Query: 309 SFILAGRDTTSSALTWFFWILSSRPHIQQKILTELETIRSRTSKQIGETYSFDELRDMHY 368
           SFI+AGRDTTS+A+TW FW+L+    +++KIL E++ +       +     F++L++M Y
Sbjct: 303 SFIMAGRDTTSAAMTWLFWLLTENDDVERKILEEVDPL-------VSLGLGFEDLKEMAY 362

Query: 369 LQAALSETLRLYPPVPVDTKACRNDDVLPDGTFIGKSWFVTYHTYAMGRMESIWGKNYGE 428
            +A L E +RLYPPV  D+K   NDDVLPDGT + +   VTY  Y MGRME++WG +  E
Sbjct: 363 TKACLCEAMRLYPPVSWDSKHAANDDVLPDGTRVKRGDKVTYFPYGMGRMETLWGTDSEE 422

Query: 429 FLPERWLEN------GVCRTESPFRFPVFHAGPRMCLGKDMAYIQMKSIAAAVMERFEVG 488
           F P RW ++       V +  SP++FPVF AGPR+C+GK+MA++QMK +  +V+ RFE+ 
Sbjct: 423 FNPNRWFDSEPGSTRPVLKPISPYKFPVFQAGPRVCVGKEMAFMQMKYVVGSVLSRFEIV 482

Query: 489 MAEKEKSPKHLLSLTLRMENGLQVIVKKR 503
              K++ P  +  LT  M  GL+V +K+R
Sbjct: 483 PVNKDR-PVFVPLLTAHMAGGLKVKIKRR 499

BLAST of Spg010521 vs. ExPASy Swiss-Prot
Match: Q9ZUX1 (Cytochrome P450 94C1 OS=Arabidopsis thaliana OX=3702 GN=CYP94C1 PE=1 SV=1)

HSP 1 Score: 404.4 bits (1038), Expect = 1.8e-111
Identity = 231/507 (45.56%), Postives = 316/507 (62.33%), Query Frame = 0

Query: 3   ISATTLFFLTFLSLSLYLLFFTSQKKSKTHQGFKHFPILGTLPLFLLNRHRFLDWSTEVL 62
           IS T + F   +  SL+ L F  QK    +    H  +  +     +N     DW T +L
Sbjct: 5   ISFTIVSFFFIIIFSLFHLLFL-QKLRYCNCEICHAYLTSSWKKDFIN---LSDWYTHLL 64

Query: 63  SSCPTNTAVFKRPGKVH---GVITANPLVVEHILKTQFENYPKGERFISLLEDFLGRGIF 122
              PT+T       KVH    VITANP  VEHILKT F NYPKG++F  +L D LGRGIF
Sbjct: 65  RRSPTSTI------KVHVLNSVITANPSNVEHILKTNFHNYPKGKQFSVILGDLLGRGIF 124

Query: 123 NSDGEIWRVQRKTASYEFNTKSLRNFVMENVRVEIQTRLLPILGKASETE-RILDLQDVL 182
           NSDG+ WR QRK AS E  + S+R F  E V+ EI+TRLLPIL   S+    +LDLQDV 
Sbjct: 125 NSDGDTWRFQRKLASLELGSVSVRVFAHEIVKTEIETRLLPILTSFSDNPGSVLDLQDVF 184

Query: 183 ERFAFDNVCKLAFDFDPGCLGGDGTAGAEFMRAFEDAATLSSGRFMYAFPGLYKVKKILN 242
            RF+FD + KL+F FDP CL       +EF  AF+ A+ LS+ R +  FP L+K K++L 
Sbjct: 185 RRFSFDTISKLSFGFDPDCLRLPFPI-SEFAVAFDTASLLSAKRALAPFPLLWKTKRLLR 244

Query: 243 IGSERTLKESIATVHKFAEDIIRSRMEEKKTTHIKNDQDLLSRFMGDEDYNSPEFLRDII 302
           IGSE+ L+ESI  +++ A D+I+ R    + T +    DL+SRFM     +  E+LRDI+
Sbjct: 245 IGSEKKLQESINVINRLAGDLIKQR----RLTGLMGKNDLISRFMAVVAEDDDEYLRDIV 304

Query: 303 ISFILAGRDTTSSALTWFFWILSSRPHIQQKILTELETIRSRTSKQIGETYSFDELRDMH 362
           +SF+LAGRDT ++ LT FFW+L+  P ++ +I  EL+ +       +  T   DE+R+M 
Sbjct: 305 VSFLLAGRDTVAAGLTGFFWLLTRHPEVENRIREELDRVMGTGFDSV--TARCDEMREMD 364

Query: 363 YLQAALSETLRLYPPVPVDTKACRNDDVLPDGTFIGKSWFVTYHTYAMGRMESIWGKNYG 422
           YL A+L E++RL+PPV  D+K   NDDVL DGTF+     VTYH YAMGRM+ IWG +Y 
Sbjct: 365 YLHASLYESMRLFPPVQFDSKFALNDDVLSDGTFVNSGTRVTYHAYAMGRMDRIWGPDYE 424

Query: 423 EFLPERWLEN-GVCRTESPFRFPVFHAGPRMCLGKDMAYIQMKSIAAAVMERFEVGMAEK 482
           EF PERWL+N G  R E+P ++PVF AG R+C+GK+MA ++MKSIA A++ RFE  +A  
Sbjct: 425 EFKPERWLDNEGKFRPENPVKYPVFQAGARVCIGKEMAIMEMKSIAVAIIRRFETRVASP 484

Query: 483 E--KSPKHLLSLTLRMENGLQVIVKKR 503
           E  ++ +    LT  +  GL V++++R
Sbjct: 485 ETTETLRFAPGLTATVNGGLPVMIQER 494

BLAST of Spg010521 vs. ExPASy Swiss-Prot
Match: Q9FMV7 (Cytochrome P450 94B1 OS=Arabidopsis thaliana OX=3702 GN=CYP94B1 PE=1 SV=1)

HSP 1 Score: 400.6 bits (1028), Expect = 2.7e-110
Identity = 228/515 (44.27%), Postives = 315/515 (61.17%), Query Frame = 0

Query: 3   ISATTLFFLTFLSLSLYLLFFTSQKKSKTHQGFK--HFPILGTLPLFLLNRHRFLDWSTE 62
           ++A  L     +   L   F T   K+KT        + ++G++  F  NRHR L W T+
Sbjct: 4   LNAIILILFPIIGFVLIFSFPTKTLKAKTASPSNPTSYQLIGSILSFNKNRHRLLQWYTD 63

Query: 63  VLSSCPTNTAVFKRPGKVHGVITANPLVVEHILKTQFENYPKGERFISLLEDFLGRGIFN 122
           +L   P+ T           +ITANP  VEHILKT F N+PKG+ F  LL D LG GIFN
Sbjct: 64  LLRLSPSQTITVDLLFGRRTIITANPENVEHILKTNFYNFPKGKPFTDLLGDLLGGGIFN 123

Query: 123 SDGEIWRVQRKTASYEFNTKSLRNFVMENVRVEIQTRLLPILGKASETERILDLQDVLER 182
           SDGE+W  QRK AS+EF  +SLR F  E +R E+Q RL+P+L  A +    +D Q+VL+R
Sbjct: 124 SDGELWSSQRKLASHEFTMRSLREFTFEILREEVQNRLIPVLSSAVDCGETVDFQEVLKR 183

Query: 183 FAFDNVCKLAFDFDPGCLGGDGTAGA-EFMRAFEDAATLSSGRFMYAFPGLYKVKKILNI 242
           FAFD VCK++  +DP CL  D T    E ++AF+ AA +S+ R       ++KVK+ LN+
Sbjct: 184 FAFDVVCKVSLGWDPDCL--DLTRPVPELVKAFDVAAEISARRATEPVYAVWKVKRFLNV 243

Query: 243 GSERTLKESIATVHKFAEDIIRSRMEEKKTTHIKND----QDLLSRFMGDEDYNSPEFLR 302
           GSE+ L+E+I TVH    +IIR++   KK+  I  D    QDLLSRF+     +  E +R
Sbjct: 244 GSEKRLREAIKTVHLSVSEIIRAK---KKSLDIGGDVSDKQDLLSRFLAAG--HGEEAVR 303

Query: 303 DIIISFILAGRDTTSSALTWFFWILSSRPHIQQKILTELETIRSRTSKQIGETYSFDELR 362
           D +ISFI+AGRDTTS+A+TW FW+LS    ++ KIL EL   R++ S  +G    F++LR
Sbjct: 304 DSVISFIMAGRDTTSAAMTWLFWLLSQNDDVETKILDEL---RNKGSLGLG----FEDLR 363

Query: 363 DMHYLQAALSETLRLYPPVPVDTKACRNDDVLPDGTFIGKSWFVTYHTYAMGRMESIWGK 422
           +M Y +A L E +RLYPPV  D+K   NDD+LPDGT + K   VTY  Y MGRME +WGK
Sbjct: 364 EMSYTKACLCEAMRLYPPVAWDSKHAANDDILPDGTPLKKGDKVTYFPYGMGRMEKVWGK 423

Query: 423 NYGEFLPERWLE-------NGVCRTESPFRFPVFHAGPRMCLGKDMAYIQMKSIAAAVME 482
           ++ EF P RW E         V ++ S F+FPVF AGPR+C+GK+MA+ QMK +  +V+ 
Sbjct: 424 DWDEFKPNRWFEEEPSYGTKPVLKSVSSFKFPVFQAGPRVCIGKEMAFTQMKYVVGSVLS 483

Query: 483 RFEVGMAEKEKSPKHLLSLTLRMENGLQVIVKKRD 504
           RF++ +      P  +  LT  M  GL+V +K+R+
Sbjct: 484 RFKI-IPVCNNRPVFVPLLTAHMAGGLKVKIKRRE 503

BLAST of Spg010521 vs. ExPASy TrEMBL
Match: A0A6J1I2N7 (cytochrome P450 94A1-like OS=Cucurbita maxima OX=3661 GN=LOC111469989 PE=3 SV=1)

HSP 1 Score: 916.4 bits (2367), Expect = 5.3e-263
Identity = 449/508 (88.39%), Postives = 479/508 (94.29%), Query Frame = 0

Query: 1   MDISATTLFFLTFLSLSLYLLFFTSQKKSKTHQGFKHFPILGTLPLFLLNRHRFLDWSTE 60
           MDISAT   FLTFLSL LYLL FTS KK K++QGFKHFPI+GTLPLFLLNRHRFLDWSTE
Sbjct: 1   MDISATAFLFLTFLSLCLYLLLFTSPKKPKSNQGFKHFPIVGTLPLFLLNRHRFLDWSTE 60

Query: 61  VLSSCPTNTAVFKRPGKVHGVITANPLVVEHILKTQFENYPKGERFISLLEDFLGRGIFN 120
           VLS+C TNTAVF+RPGKVHGVITANPLVVEHILKTQFENYPKG+RFISLLEDFLGRGIFN
Sbjct: 61  VLSNCQTNTAVFRRPGKVHGVITANPLVVEHILKTQFENYPKGKRFISLLEDFLGRGIFN 120

Query: 121 SDGEIWRVQRKTASYEFNTKSLRNFVMENVRVEIQTRLLPILGKASETERILDLQDVLER 180
           SDGEIW+VQRKTASYEFNTKSLRNFVMENV VEIQTRLLPILGKASETERILDLQDVLER
Sbjct: 121 SDGEIWKVQRKTASYEFNTKSLRNFVMENVTVEIQTRLLPILGKASETERILDLQDVLER 180

Query: 181 FAFDNVCKLAFDFDPGCLGGDGTAGAEFMRAFEDAATLSSGRFMYAFPGLYKVKKILNIG 240
           FAFDNVCKLAF+FDP CLGGDGT+GA+FMRAFEDAATLSSGRFMYA PGLYKVKK LN+G
Sbjct: 181 FAFDNVCKLAFNFDPCCLGGDGTSGADFMRAFEDAATLSSGRFMYALPGLYKVKKFLNMG 240

Query: 241 SERTLKESIATVHKFAEDIIRSRMEEKKTTHIKNDQDLLSRFMGDEDYNSPEFLRDIIIS 300
           SERTLK+SIA VHKFAEDIIRSRMEEKKTTH+KN+QDLLSRFMG ++ NSPEFLRDIIIS
Sbjct: 241 SERTLKKSIARVHKFAEDIIRSRMEEKKTTHVKNNQDLLSRFMGTQENNSPEFLRDIIIS 300

Query: 301 FILAGRDTTSSALTWFFWILSSRPHIQQKILTELETIRSRTSKQIGETYSFDELRDMHYL 360
           FILAGRDTTSSALTWFFWILSSRP+I QKIL ELETIRSRT K++G  YSF+ELRDMHYL
Sbjct: 301 FILAGRDTTSSALTWFFWILSSRPNITQKILKELETIRSRTGKKLGAEYSFEELRDMHYL 360

Query: 361 QAALSETLRLYPPVPVDTKACRNDDVLPDGTFIGKSWFVTYHTYAMGRMESIWGKNYGEF 420
           QAALSETLRLYPPVPVDTKACRN+DVLPDGT +G+ WFVTYHTYAMGRME IWG+N GEF
Sbjct: 361 QAALSETLRLYPPVPVDTKACRNEDVLPDGTLVGRDWFVTYHTYAMGRMERIWGENCGEF 420

Query: 421 LPERWLENGVCRTESPFRFPVFHAGPRMCLGKDMAYIQMKSIAAAVMERFEVGMAEKEKS 480
           LPERW+ENGVCR ESPFRFPVFHAGPRMCLGKDMAYIQMKSIAAAV+ERFEVGMAEKEKS
Sbjct: 421 LPERWVENGVCRAESPFRFPVFHAGPRMCLGKDMAYIQMKSIAAAVIERFEVGMAEKEKS 480

Query: 481 PKHLLSLTLRMENGLQVIVKKRDTSVTI 509
           PK+LLSLTLRMENG  V++KKRD S+ I
Sbjct: 481 PKYLLSLTLRMENGCPVVLKKRDPSLAI 508

BLAST of Spg010521 vs. ExPASy TrEMBL
Match: A0A6J1EZ74 (cytochrome P450 94A1-like OS=Cucurbita moschata OX=3662 GN=LOC111440896 PE=3 SV=1)

HSP 1 Score: 916.0 bits (2366), Expect = 6.9e-263
Identity = 448/508 (88.19%), Postives = 479/508 (94.29%), Query Frame = 0

Query: 1   MDISATTLFFLTFLSLSLYLLFFTSQKKSKTHQGFKHFPILGTLPLFLLNRHRFLDWSTE 60
           MDISAT   FLTFLSL LYLL F S KKSK++QGFKHFPI+GTLPLFLLNRHRFLDWSTE
Sbjct: 1   MDISATAFLFLTFLSLCLYLLLFASPKKSKSNQGFKHFPIVGTLPLFLLNRHRFLDWSTE 60

Query: 61  VLSSCPTNTAVFKRPGKVHGVITANPLVVEHILKTQFENYPKGERFISLLEDFLGRGIFN 120
           VLS+C TNTAVF+RPGKVHGVITANPLVVEHILKTQFENYPKG+RFISLLEDFLGRGIFN
Sbjct: 61  VLSNCQTNTAVFRRPGKVHGVITANPLVVEHILKTQFENYPKGKRFISLLEDFLGRGIFN 120

Query: 121 SDGEIWRVQRKTASYEFNTKSLRNFVMENVRVEIQTRLLPILGKASETERILDLQDVLER 180
           SDGEIW+VQRKTASYEFNTKSLRNFVMENV VEIQTRLLPILGKASETERILDLQDVLER
Sbjct: 121 SDGEIWKVQRKTASYEFNTKSLRNFVMENVTVEIQTRLLPILGKASETERILDLQDVLER 180

Query: 181 FAFDNVCKLAFDFDPGCLGGDGTAGAEFMRAFEDAATLSSGRFMYAFPGLYKVKKILNIG 240
           FAFDNVCKLAF+FDP CLGGDGT+GA+FMRAFEDAATLSSGRFMYA PGLYKVKK LN+G
Sbjct: 181 FAFDNVCKLAFNFDPCCLGGDGTSGADFMRAFEDAATLSSGRFMYALPGLYKVKKFLNMG 240

Query: 241 SERTLKESIATVHKFAEDIIRSRMEEKKTTHIKNDQDLLSRFMGDEDYNSPEFLRDIIIS 300
           SERTLK+SIA VHKFAEDIIRSRMEEKKTTH+KNDQDLLSRFMG ++ NSPEFLRDIIIS
Sbjct: 241 SERTLKKSIARVHKFAEDIIRSRMEEKKTTHVKNDQDLLSRFMGSQENNSPEFLRDIIIS 300

Query: 301 FILAGRDTTSSALTWFFWILSSRPHIQQKILTELETIRSRTSKQIGETYSFDELRDMHYL 360
           FILAGRDTTSSALTWFFWILSSRP+I QKIL ELETIRSRT K++G  YSF+ELRDMHYL
Sbjct: 301 FILAGRDTTSSALTWFFWILSSRPNITQKILKELETIRSRTGKKLGAEYSFEELRDMHYL 360

Query: 361 QAALSETLRLYPPVPVDTKACRNDDVLPDGTFIGKSWFVTYHTYAMGRMESIWGKNYGEF 420
           QAALSETLRLYPPVPVDTKACRN+DVLPDGT +G+ WFVTYHTYAMGRME IWG+N GEF
Sbjct: 361 QAALSETLRLYPPVPVDTKACRNEDVLPDGTLVGREWFVTYHTYAMGRMERIWGENCGEF 420

Query: 421 LPERWLENGVCRTESPFRFPVFHAGPRMCLGKDMAYIQMKSIAAAVMERFEVGMAEKEKS 480
           LPERW+ENGVCR ESPFRFPVFHAGPRMCLGK+MAYIQMKSIAAAV+ERFEVGMAEKEKS
Sbjct: 421 LPERWVENGVCRAESPFRFPVFHAGPRMCLGKEMAYIQMKSIAAAVIERFEVGMAEKEKS 480

Query: 481 PKHLLSLTLRMENGLQVIVKKRDTSVTI 509
           PK+LLSLTLRMENG  V++K+RD S+ I
Sbjct: 481 PKYLLSLTLRMENGCPVVLKRRDPSLAI 508

BLAST of Spg010521 vs. ExPASy TrEMBL
Match: A0A5D3DPN2 (Cytochrome P450 94A1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G006500 PE=3 SV=1)

HSP 1 Score: 896.7 bits (2316), Expect = 4.4e-257
Identity = 440/507 (86.79%), Postives = 470/507 (92.70%), Query Frame = 0

Query: 3   ISATTLFFLTFLSLSLYLLFFTSQK-KSKTHQGFKHFPILGTLPLFLLNRHRFLDWSTEV 62
           +S T   FLTF S S+Y LFFTS K KSK  QGFKHFP +GTLPLFLLNRHRFLDWSTEV
Sbjct: 6   LSLTPFIFLTFFSFSIYFLFFTSTKSKSKPQQGFKHFPFVGTLPLFLLNRHRFLDWSTEV 65

Query: 63  LSSCPTNTAVFKRPGKVHGVITANPLVVEHILKTQFENYPKGERFISLLEDFLGRGIFNS 122
           L +C TNTAVFKRPGKVHGVITANPLVVEHILKTQFENYPKGERFISLLEDFLGRGIFNS
Sbjct: 66  LRNCRTNTAVFKRPGKVHGVITANPLVVEHILKTQFENYPKGERFISLLEDFLGRGIFNS 125

Query: 123 DGEIWRVQRKTASYEFNTKSLRNFVMENVRVEIQTRLLPILGKASETERILDLQDVLERF 182
           DGEIW+VQRKTASYEFNTKSLRNFVMENVRVEIQ+RLLPI GKA ETERILDLQDVLERF
Sbjct: 126 DGEIWKVQRKTASYEFNTKSLRNFVMENVRVEIQSRLLPIFGKACETERILDLQDVLERF 185

Query: 183 AFDNVCKLAFDFDPGCLGGDGTAGAEFMRAFEDAATLSSGRFMYAFPGLYKVKKILNIGS 242
           AFDNVCKLAF++DP CLGGDGTA  EFMRAFE AATLSSGRFMYAFPG YKVKK  NIGS
Sbjct: 186 AFDNVCKLAFNYDPACLGGDGTAATEFMRAFEHAATLSSGRFMYAFPGSYKVKKFFNIGS 245

Query: 243 ERTLKESIATVHKFAEDIIRSRMEEKKTTHIKNDQDLLSRFMGDEDYNSPEFLRDIIISF 302
           E+TLKESIA VHKFAEDII SR+EEKKTT I+NDQDLLSRFMGD++ NSP+FLRDIIISF
Sbjct: 246 EKTLKESIAVVHKFAEDIIHSRLEEKKTTQIENDQDLLSRFMGDQN-NSPQFLRDIIISF 305

Query: 303 ILAGRDTTSSALTWFFWILSSRPHIQQKILTELETIRSRTSKQIGETYSFDELRDMHYLQ 362
           ILAGRDTTSSALTWFFWILSSRP ++QKIL ELETIR++  K+IGE YSFDELRDMHYLQ
Sbjct: 306 ILAGRDTTSSALTWFFWILSSRPDVEQKILAELETIRTKAHKEIGEMYSFDELRDMHYLQ 365

Query: 363 AALSETLRLYPPVPVDTKACRNDDVLPDGTFIGKSWFVTYHTYAMGRMESIWGKNYGEFL 422
           AALSETLRLYPPVPVDTKACRNDDVLPDGTFIGKSWFVTYHTYAMGRMESIWGK+YGEF 
Sbjct: 366 AALSETLRLYPPVPVDTKACRNDDVLPDGTFIGKSWFVTYHTYAMGRMESIWGKDYGEFS 425

Query: 423 PERWLENGVCRTESPFRFPVFHAGPRMCLGKDMAYIQMKSIAAAVMERFEVGMAEKEKSP 482
           PERWLENGVC+TESPFRFP+FHAGPRMCLGKDMAYIQMKSIAAAV+E+FEV M EK+KSP
Sbjct: 426 PERWLENGVCKTESPFRFPIFHAGPRMCLGKDMAYIQMKSIAAAVIEKFEVEMVEKKKSP 485

Query: 483 KHLLSLTLRMENGLQVIVKKRDTSVTI 509
           KHLLSLTLRMENGLQV++K+RD  ++I
Sbjct: 486 KHLLSLTLRMENGLQVMIKRRDRGLSI 511

BLAST of Spg010521 vs. ExPASy TrEMBL
Match: A0A1S3B728 (cytochrome P450 94A1-like OS=Cucumis melo OX=3656 GN=LOC103486871 PE=3 SV=1)

HSP 1 Score: 896.7 bits (2316), Expect = 4.4e-257
Identity = 440/507 (86.79%), Postives = 470/507 (92.70%), Query Frame = 0

Query: 3   ISATTLFFLTFLSLSLYLLFFTSQK-KSKTHQGFKHFPILGTLPLFLLNRHRFLDWSTEV 62
           +S T   FLTF S S+Y LFFTS K KSK  QGFKHFP +GTLPLFLLNRHRFLDWSTEV
Sbjct: 6   LSLTPFIFLTFFSFSIYFLFFTSTKSKSKPQQGFKHFPFVGTLPLFLLNRHRFLDWSTEV 65

Query: 63  LSSCPTNTAVFKRPGKVHGVITANPLVVEHILKTQFENYPKGERFISLLEDFLGRGIFNS 122
           L +C TNTAVFKRPGKVHGVITANPLVVEHILKTQFENYPKGERFISLLEDFLGRGIFNS
Sbjct: 66  LRNCRTNTAVFKRPGKVHGVITANPLVVEHILKTQFENYPKGERFISLLEDFLGRGIFNS 125

Query: 123 DGEIWRVQRKTASYEFNTKSLRNFVMENVRVEIQTRLLPILGKASETERILDLQDVLERF 182
           DGEIW+VQRKTASYEFNTKSLRNFVMENVRVEIQ+RLLPI GKA ETERILDLQDVLERF
Sbjct: 126 DGEIWKVQRKTASYEFNTKSLRNFVMENVRVEIQSRLLPIFGKACETERILDLQDVLERF 185

Query: 183 AFDNVCKLAFDFDPGCLGGDGTAGAEFMRAFEDAATLSSGRFMYAFPGLYKVKKILNIGS 242
           AFDNVCKLAF++DP CLGGDGTA  EFMRAFE AATLSSGRFMYAFPG YKVKK  NIGS
Sbjct: 186 AFDNVCKLAFNYDPACLGGDGTAATEFMRAFEHAATLSSGRFMYAFPGSYKVKKFFNIGS 245

Query: 243 ERTLKESIATVHKFAEDIIRSRMEEKKTTHIKNDQDLLSRFMGDEDYNSPEFLRDIIISF 302
           E+TLKESIA VHKFAEDII SR+EEKKTT I+NDQDLLSRFMGD++ NSP+FLRDIIISF
Sbjct: 246 EKTLKESIAVVHKFAEDIIHSRLEEKKTTQIENDQDLLSRFMGDQN-NSPQFLRDIIISF 305

Query: 303 ILAGRDTTSSALTWFFWILSSRPHIQQKILTELETIRSRTSKQIGETYSFDELRDMHYLQ 362
           ILAGRDTTSSALTWFFWILSSRP ++QKIL ELETIR++  K+IGE YSFDELRDMHYLQ
Sbjct: 306 ILAGRDTTSSALTWFFWILSSRPDVEQKILAELETIRTKAHKEIGEMYSFDELRDMHYLQ 365

Query: 363 AALSETLRLYPPVPVDTKACRNDDVLPDGTFIGKSWFVTYHTYAMGRMESIWGKNYGEFL 422
           AALSETLRLYPPVPVDTKACRNDDVLPDGTFIGKSWFVTYHTYAMGRMESIWGK+YGEF 
Sbjct: 366 AALSETLRLYPPVPVDTKACRNDDVLPDGTFIGKSWFVTYHTYAMGRMESIWGKDYGEFS 425

Query: 423 PERWLENGVCRTESPFRFPVFHAGPRMCLGKDMAYIQMKSIAAAVMERFEVGMAEKEKSP 482
           PERWLENGVC+TESPFRFP+FHAGPRMCLGKDMAYIQMKSIAAAV+E+FEV M EK+KSP
Sbjct: 426 PERWLENGVCKTESPFRFPIFHAGPRMCLGKDMAYIQMKSIAAAVIEKFEVEMVEKKKSP 485

Query: 483 KHLLSLTLRMENGLQVIVKKRDTSVTI 509
           KHLLSLTLRMENGLQV++K+RD  ++I
Sbjct: 486 KHLLSLTLRMENGLQVMIKRRDRGLSI 511

BLAST of Spg010521 vs. ExPASy TrEMBL
Match: A0A0A0LFF3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G818260 PE=3 SV=1)

HSP 1 Score: 883.6 bits (2282), Expect = 3.8e-253
Identity = 435/512 (84.96%), Postives = 470/512 (91.80%), Query Frame = 0

Query: 1   MDISATTL---FFLTFLSLSLYLLFFTSQK-KSKTHQGFKHFPILGTLPLFLLNRHRFLD 60
           MDIS  TL    FLTF S S+Y LFF S K KSK  QGFKH+P +GTLPLFLLNRHRFLD
Sbjct: 1   MDISVLTLTPFIFLTFFSFSIYFLFFPSTKSKSKPQQGFKHYPFVGTLPLFLLNRHRFLD 60

Query: 61  WSTEVLSSCPTNTAVFKRPGKVHGVITANPLVVEHILKTQFENYPKGERFISLLEDFLGR 120
           WSTEVL +C TNT+VFKRPGKVHGVITANPLVVEHILKTQFENYPKGERFISLLEDFLGR
Sbjct: 61  WSTEVLRNCRTNTSVFKRPGKVHGVITANPLVVEHILKTQFENYPKGERFISLLEDFLGR 120

Query: 121 GIFNSDGEIWRVQRKTASYEFNTKSLRNFVMENVRVEIQTRLLPILGKASETERILDLQD 180
           GIFNSDGEIW+VQRKTASYEFNTKSLRNFVMENVRVEIQ+RLLPI GKA ETERILDLQD
Sbjct: 121 GIFNSDGEIWKVQRKTASYEFNTKSLRNFVMENVRVEIQSRLLPIFGKACETERILDLQD 180

Query: 181 VLERFAFDNVCKLAFDFDPGCLGGDGTAGAEFMRAFEDAATLSSGRFMYAFPGLYKVKKI 240
           VLERFAFDNVCKLAF++DP CLGG GT+ AEFMRAFEDAA LS+GRFMYAFPGLYKVKK 
Sbjct: 181 VLERFAFDNVCKLAFNYDPACLGGGGTSAAEFMRAFEDAANLSAGRFMYAFPGLYKVKKF 240

Query: 241 LNIGSERTLKESIATVHKFAEDIIRSRMEEKKTTHIKNDQDLLSRFMGDEDYNSPEFLRD 300
            NIGSE+TL ESIA VHKFAEDII SR+EEKKTT I+NDQDLLSRFMGD++ NSP+FLRD
Sbjct: 241 FNIGSEKTLNESIAIVHKFAEDIIHSRLEEKKTTQIENDQDLLSRFMGDQN-NSPQFLRD 300

Query: 301 IIISFILAGRDTTSSALTWFFWILSSRPHIQQKILTELETIRSRTSKQIGETYSFDELRD 360
           IIISFILAGRDTTSSALTWFFWILSSR  +QQKIL ELETIR++  K+IGE YSFDELRD
Sbjct: 301 IIISFILAGRDTTSSALTWFFWILSSRHDVQQKILAELETIRTKARKEIGEMYSFDELRD 360

Query: 361 MHYLQAALSETLRLYPPVPVDTKACRNDDVLPDGTFIGKSWFVTYHTYAMGRMESIWGKN 420
           MHYLQAA+SETLRLYPPVPVDTKACRNDDVLPDGTFIGKSWFVTYHTYAMGRMESIWGK+
Sbjct: 361 MHYLQAAISETLRLYPPVPVDTKACRNDDVLPDGTFIGKSWFVTYHTYAMGRMESIWGKD 420

Query: 421 YGEFLPERWLENGVCRTESPFRFPVFHAGPRMCLGKDMAYIQMKSIAAAVMERFEVGMAE 480
           YGE+ PERWLENGVC+TESPFRFP+FHAGPRMCLGKDMAYIQMK IAAAV+E+FEV M E
Sbjct: 421 YGEYSPERWLENGVCKTESPFRFPIFHAGPRMCLGKDMAYIQMKCIAAAVIEKFEVEMVE 480

Query: 481 KEKSPKHLLSLTLRMENGLQVIVKKRDTSVTI 509
           K+KSPKHLLSLTLRMENGL+V++KKR+  ++I
Sbjct: 481 KKKSPKHLLSLTLRMENGLEVMIKKRERGLSI 511

BLAST of Spg010521 vs. TAIR 10
Match: AT3G56630.1 (cytochrome P450, family 94, subfamily D, polypeptide 2 )

HSP 1 Score: 630.9 bits (1626), Expect = 8.6e-181
Identity = 305/494 (61.74%), Postives = 389/494 (78.74%), Query Frame = 0

Query: 11  LTFLSLSLYLLFFTSQKKSKTHQGFKHFPILGTLPLFLLNRHRFLDWSTEVLSSCPTNTA 70
           ++ L   + + F    K S +  GFK +PI+G+LP  + NRHRFLDW+ E LS CPT TA
Sbjct: 7   ISLLCFPIVIFFIFFPKNSSSEFGFKSYPIVGSLPGLVNNRHRFLDWTVETLSRCPTQTA 66

Query: 71  VFKRPGKVHGVITANPLVVEHILKTQFENYPKGERFISLLEDFLGRGIFNSDGEIWRVQR 130
           +F+RPGK+  V+TANP  VE++LKT+FE++PKGERFIS+LEDFLGRGIFNSDGE+W  QR
Sbjct: 67  IFRRPGKLQFVMTANPANVEYMLKTKFESFPKGERFISILEDFLGRGIFNSDGEMWWKQR 126

Query: 131 KTASYEFNTKSLRNFVMENVRVEIQTRLLPILGKASETERILDLQDVLERFAFDNVCKLA 190
           KTASYEF+TKSLR+FVM NV VEI TRL+P+L +A+   +++DLQD+LERFAFDN+CKLA
Sbjct: 127 KTASYEFSTKSLRDFVMSNVTVEINTRLVPVLAEAATNGKLIDLQDILERFAFDNICKLA 186

Query: 191 FDFDPGCLGGDGTAGAEFMRAFEDAATLSSGRFMYAFPGLYKVKKILNIGSERTLKESIA 250
           F+ D  CLG DG AG  FM+AFE AAT+ S RF       +K+KK LNIGSER L+ESI 
Sbjct: 187 FNVDSACLGDDGAAGVNFMQAFETAATIISQRFQSVISYSWKIKKKLNIGSERVLRESIM 246

Query: 251 TVHKFAEDIIRSRMEEKKTTHIKNDQDLLSRFMGDEDYNSPEFLRDIIISFILAGRDTTS 310
            VHKFA++I+R+R+E+ K +  K  +DLLSRF+  E+ NSPE LRDI+ISFILAGRDTTS
Sbjct: 247 IVHKFADEIVRNRIEQGKVSDHK--EDLLSRFISKEEMNSPEILRDIVISFILAGRDTTS 306

Query: 311 SALTWFFWILSSRPHIQQKILTELETIRSRTSKQIGETYSFDELRDMHYLQAALSETLRL 370
           SAL+WFFW+LS  P ++ KIL EL +IR RT K+IGE Y F++L+ M+YL AA++E+LRL
Sbjct: 307 SALSWFFWLLSMHPEVKDKILQELNSIRERTGKRIGEVYGFEDLKLMNYLHAAITESLRL 366

Query: 371 YPPVPVDTKACRNDDVLPDGTFIGKSWFVTYHTYAMGRMESIWGKNYGEFLPERWLE--N 430
           YPPVPVDT +C  D+VLPDGTFIGK W ++Y+ YAMGRMESIWGK+   F PERW++  N
Sbjct: 367 YPPVPVDTMSCAEDNVLPDGTFIGKDWGISYNAYAMGRMESIWGKDCDRFDPERWIDETN 426

Query: 431 GVCRTESPFRFPVFHAGPRMCLGKDMAYIQMKSIAAAVMERFEVGMAEKEKSPKHLLSLT 490
           G  R E+P++FP FHAGPRMCLGK+MAYIQMKSI AAV+ERF V +  K++ P+ L+S+T
Sbjct: 427 GGFRGENPYKFPAFHAGPRMCLGKEMAYIQMKSIVAAVLERFVVEVPGKKERPEILMSVT 486

Query: 491 LRMENGLQVIVKKR 503
           LR+  GL V V++R
Sbjct: 487 LRIRGGLNVRVQER 498

BLAST of Spg010521 vs. TAIR 10
Match: AT1G34540.1 (cytochrome P450, family 94, subfamily D, polypeptide 1 )

HSP 1 Score: 620.5 bits (1599), Expect = 1.2e-177
Identity = 303/495 (61.21%), Postives = 391/495 (78.99%), Query Frame = 0

Query: 10  FLTFLSLSLYLLFFTSQKKSKTHQGFKHFPILGTLPLFLLNRHRFLDWSTEVLSSCPTNT 69
           FL    +S++ +FFT  KKS +  GFK +PI+G+ P  + NRHRFLDW+ E LS CPT T
Sbjct: 8   FLLCFPISIFFIFFT--KKSSSEFGFKSYPIVGSFPGLVNNRHRFLDWTVETLSRCPTQT 67

Query: 70  AVFKRPGKVHGVITANPLVVEHILKTQFENYPKGERFISLLEDFLGRGIFNSDGEIWRVQ 129
           A+F+RPGK   ++TANP  VE++LKT+FE++PKG++F S+LEDFLG GIFNSDG++W  Q
Sbjct: 68  AIFRRPGKQQLIMTANPSNVEYMLKTKFESFPKGQQFTSVLEDFLGHGIFNSDGDMWWKQ 127

Query: 130 RKTASYEFNTKSLRNFVMENVRVEIQTRLLPILGKASETERILDLQDVLERFAFDNVCKL 189
           RKTASYEF+TKSLR+FVM NV VEI TRL+P+L +A+ T +++DLQD+LERFAFDN+CKL
Sbjct: 128 RKTASYEFSTKSLRDFVMSNVTVEINTRLVPVLVEAATTGKLIDLQDILERFAFDNICKL 187

Query: 190 AFDFDPGCLGGDGTAGAEFMRAFEDAATLSSGRFMYAFPGLYKVKKILNIGSERTLKESI 249
           AF+ D  CLG DG  G  FMRAFE AAT+ S RF       +++KK LNIGSER L+ESI
Sbjct: 188 AFNVDCACLGHDGAVGVNFMRAFETAATIISQRFRSVASCAWRIKKKLNIGSERVLRESI 247

Query: 250 ATVHKFAEDIIRSRMEEKKTTHIKNDQDLLSRFMGDEDYNSPEFLRDIIISFILAGRDTT 309
           ATVHKFA++I+R+R+++ +++  K  +DLLSRF+  E+ NSPE LRDI+ISFILAGRDTT
Sbjct: 248 ATVHKFADEIVRNRIDQGRSSDHK--EDLLSRFISKEEMNSPEILRDIVISFILAGRDTT 307

Query: 310 SSALTWFFWILSSRPHIQQKILTELETIRSRTSKQIGETYSFDELRDMHYLQAALSETLR 369
           SSAL+WFFW+LS  P ++ KIL EL +IR+RT K+IGE Y F+ L+ M+YL AA++E+LR
Sbjct: 308 SSALSWFFWLLSMHPEVEDKILQELNSIRARTGKRIGEVYGFEHLKMMNYLHAAITESLR 367

Query: 370 LYPPVPVDTKACRNDDVLPDGTFIGKSWFVTYHTYAMGRMESIWGKNYGEFLPERWLE-- 429
           LYPPVPVD K+C  D+VLPDGTF+GK W +TY+ +AMGRMESIWGK+   F PERW++  
Sbjct: 368 LYPPVPVDIKSCAEDNVLPDGTFVGKGWAITYNIFAMGRMESIWGKDCDRFDPERWIDET 427

Query: 430 NGVCRTESPFRFPVFHAGPRMCLGKDMAYIQMKSIAAAVMERFEVGMAEKEKSPKHLLSL 489
           NG  R E P +FP FHAGPRMC+GKDMAYIQMKSI AAV+ERF V +  KE+ P+ LLS+
Sbjct: 428 NGCFRGEDPSKFPAFHAGPRMCVGKDMAYIQMKSIVAAVLERFVVEVPGKER-PEILLSM 487

Query: 490 TLRMENGLQVIVKKR 503
           TLR++ GL   V++R
Sbjct: 488 TLRIKGGLFARVQER 497

BLAST of Spg010521 vs. TAIR 10
Match: AT3G48520.1 (cytochrome P450, family 94, subfamily B, polypeptide 3 )

HSP 1 Score: 407.9 bits (1047), Expect = 1.2e-113
Identity = 221/509 (43.42%), Postives = 324/509 (63.65%), Query Frame = 0

Query: 9   FFLTFLSLS--LYLLFFTSQKKSK-----THQGFKHFPILGTLPLFLLNRHRFLDWSTEV 68
           F L+FL L+  + ++FF S   +K     T  G   +P++G++  F  NRHR L W TE+
Sbjct: 3   FLLSFLILAFLITIIFFLSSSSTKKVQENTTYGPPSYPLIGSILSFNKNRHRLLQWYTEL 62

Query: 69  LSSCPTNTAVFKRPGKVHGVITANPLVVEHILKTQFENYPKGERFISLLEDFLGRGIFNS 128
           L   P+ T +    G    +IT NPL VE+ILKT F N+PKG+ F  LL D LG GIFN 
Sbjct: 63  LRLSPSQTILVPLLGNRRTIITTNPLNVEYILKTNFFNFPKGKPFTDLLGDLLGGGIFNV 122

Query: 129 DGEIWRVQRKTASYEFNTKSLRNFVMENVRVEIQTRLLPILGKASETERILDLQDVLERF 188
           DG  W  QRK AS+EF+T+SLR+F  E ++ E++ RL+P+L  A++    +DLQDVL+RF
Sbjct: 123 DGHSWSSQRKLASHEFSTRSLRSFAFEVLKDEVENRLVPVLSTAADVGTTVDLQDVLKRF 182

Query: 189 AFDNVCKLAFDFDPGCLGGDGTAGAE-FMRAFEDAATLSSGRFMYAFPGLYKVKKILNIG 248
           AFD VCK++  +DP CL  D T      + AF+ AA +S+ R       ++K K++LN+G
Sbjct: 183 AFDVVCKVSLGWDPDCL--DLTRPVNPLVEAFDTAAEISARRATEPIYAVWKTKRVLNVG 242

Query: 249 SERTLKESIATVHKFAEDIIRSRMEEKKT-THIKNDQDLLSRFMGDEDYNSPEFLRDIII 308
           SER L+E+I TVH    +I+R++ +  +  T  +  QDLLSRF+     ++ E +RD++I
Sbjct: 243 SERKLREAIRTVHVLVSEIVRAKKKSLEIGTGAEAKQDLLSRFLAAG--HNGEAVRDMVI 302

Query: 309 SFILAGRDTTSSALTWFFWILSSRPHIQQKILTELETIRSRTSKQIGETYSFDELRDMHY 368
           SFI+AGRDTTS+A+TW FW+L+    +++KIL E++ +       +     F++L++M Y
Sbjct: 303 SFIMAGRDTTSAAMTWLFWLLTENDDVERKILEEVDPL-------VSLGLGFEDLKEMAY 362

Query: 369 LQAALSETLRLYPPVPVDTKACRNDDVLPDGTFIGKSWFVTYHTYAMGRMESIWGKNYGE 428
            +A L E +RLYPPV  D+K   NDDVLPDGT + +   VTY  Y MGRME++WG +  E
Sbjct: 363 TKACLCEAMRLYPPVSWDSKHAANDDVLPDGTRVKRGDKVTYFPYGMGRMETLWGTDSEE 422

Query: 429 FLPERWLEN------GVCRTESPFRFPVFHAGPRMCLGKDMAYIQMKSIAAAVMERFEVG 488
           F P RW ++       V +  SP++FPVF AGPR+C+GK+MA++QMK +  +V+ RFE+ 
Sbjct: 423 FNPNRWFDSEPGSTRPVLKPISPYKFPVFQAGPRVCVGKEMAFMQMKYVVGSVLSRFEIV 482

Query: 489 MAEKEKSPKHLLSLTLRMENGLQVIVKKR 503
              K++ P  +  LT  M  GL+V +K+R
Sbjct: 483 PVNKDR-PVFVPLLTAHMAGGLKVKIKRR 499

BLAST of Spg010521 vs. TAIR 10
Match: AT2G27690.1 (cytochrome P450, family 94, subfamily C, polypeptide 1 )

HSP 1 Score: 404.4 bits (1038), Expect = 1.3e-112
Identity = 231/507 (45.56%), Postives = 316/507 (62.33%), Query Frame = 0

Query: 3   ISATTLFFLTFLSLSLYLLFFTSQKKSKTHQGFKHFPILGTLPLFLLNRHRFLDWSTEVL 62
           IS T + F   +  SL+ L F  QK    +    H  +  +     +N     DW T +L
Sbjct: 5   ISFTIVSFFFIIIFSLFHLLFL-QKLRYCNCEICHAYLTSSWKKDFIN---LSDWYTHLL 64

Query: 63  SSCPTNTAVFKRPGKVH---GVITANPLVVEHILKTQFENYPKGERFISLLEDFLGRGIF 122
              PT+T       KVH    VITANP  VEHILKT F NYPKG++F  +L D LGRGIF
Sbjct: 65  RRSPTSTI------KVHVLNSVITANPSNVEHILKTNFHNYPKGKQFSVILGDLLGRGIF 124

Query: 123 NSDGEIWRVQRKTASYEFNTKSLRNFVMENVRVEIQTRLLPILGKASETE-RILDLQDVL 182
           NSDG+ WR QRK AS E  + S+R F  E V+ EI+TRLLPIL   S+    +LDLQDV 
Sbjct: 125 NSDGDTWRFQRKLASLELGSVSVRVFAHEIVKTEIETRLLPILTSFSDNPGSVLDLQDVF 184

Query: 183 ERFAFDNVCKLAFDFDPGCLGGDGTAGAEFMRAFEDAATLSSGRFMYAFPGLYKVKKILN 242
            RF+FD + KL+F FDP CL       +EF  AF+ A+ LS+ R +  FP L+K K++L 
Sbjct: 185 RRFSFDTISKLSFGFDPDCLRLPFPI-SEFAVAFDTASLLSAKRALAPFPLLWKTKRLLR 244

Query: 243 IGSERTLKESIATVHKFAEDIIRSRMEEKKTTHIKNDQDLLSRFMGDEDYNSPEFLRDII 302
           IGSE+ L+ESI  +++ A D+I+ R    + T +    DL+SRFM     +  E+LRDI+
Sbjct: 245 IGSEKKLQESINVINRLAGDLIKQR----RLTGLMGKNDLISRFMAVVAEDDDEYLRDIV 304

Query: 303 ISFILAGRDTTSSALTWFFWILSSRPHIQQKILTELETIRSRTSKQIGETYSFDELRDMH 362
           +SF+LAGRDT ++ LT FFW+L+  P ++ +I  EL+ +       +  T   DE+R+M 
Sbjct: 305 VSFLLAGRDTVAAGLTGFFWLLTRHPEVENRIREELDRVMGTGFDSV--TARCDEMREMD 364

Query: 363 YLQAALSETLRLYPPVPVDTKACRNDDVLPDGTFIGKSWFVTYHTYAMGRMESIWGKNYG 422
           YL A+L E++RL+PPV  D+K   NDDVL DGTF+     VTYH YAMGRM+ IWG +Y 
Sbjct: 365 YLHASLYESMRLFPPVQFDSKFALNDDVLSDGTFVNSGTRVTYHAYAMGRMDRIWGPDYE 424

Query: 423 EFLPERWLEN-GVCRTESPFRFPVFHAGPRMCLGKDMAYIQMKSIAAAVMERFEVGMAEK 482
           EF PERWL+N G  R E+P ++PVF AG R+C+GK+MA ++MKSIA A++ RFE  +A  
Sbjct: 425 EFKPERWLDNEGKFRPENPVKYPVFQAGARVCIGKEMAIMEMKSIAVAIIRRFETRVASP 484

Query: 483 E--KSPKHLLSLTLRMENGLQVIVKKR 503
           E  ++ +    LT  +  GL V++++R
Sbjct: 485 ETTETLRFAPGLTATVNGGLPVMIQER 494

BLAST of Spg010521 vs. TAIR 10
Match: AT5G63450.1 (cytochrome P450, family 94, subfamily B, polypeptide 1 )

HSP 1 Score: 400.6 bits (1028), Expect = 1.9e-111
Identity = 228/515 (44.27%), Postives = 315/515 (61.17%), Query Frame = 0

Query: 3   ISATTLFFLTFLSLSLYLLFFTSQKKSKTHQGFK--HFPILGTLPLFLLNRHRFLDWSTE 62
           ++A  L     +   L   F T   K+KT        + ++G++  F  NRHR L W T+
Sbjct: 4   LNAIILILFPIIGFVLIFSFPTKTLKAKTASPSNPTSYQLIGSILSFNKNRHRLLQWYTD 63

Query: 63  VLSSCPTNTAVFKRPGKVHGVITANPLVVEHILKTQFENYPKGERFISLLEDFLGRGIFN 122
           +L   P+ T           +ITANP  VEHILKT F N+PKG+ F  LL D LG GIFN
Sbjct: 64  LLRLSPSQTITVDLLFGRRTIITANPENVEHILKTNFYNFPKGKPFTDLLGDLLGGGIFN 123

Query: 123 SDGEIWRVQRKTASYEFNTKSLRNFVMENVRVEIQTRLLPILGKASETERILDLQDVLER 182
           SDGE+W  QRK AS+EF  +SLR F  E +R E+Q RL+P+L  A +    +D Q+VL+R
Sbjct: 124 SDGELWSSQRKLASHEFTMRSLREFTFEILREEVQNRLIPVLSSAVDCGETVDFQEVLKR 183

Query: 183 FAFDNVCKLAFDFDPGCLGGDGTAGA-EFMRAFEDAATLSSGRFMYAFPGLYKVKKILNI 242
           FAFD VCK++  +DP CL  D T    E ++AF+ AA +S+ R       ++KVK+ LN+
Sbjct: 184 FAFDVVCKVSLGWDPDCL--DLTRPVPELVKAFDVAAEISARRATEPVYAVWKVKRFLNV 243

Query: 243 GSERTLKESIATVHKFAEDIIRSRMEEKKTTHIKND----QDLLSRFMGDEDYNSPEFLR 302
           GSE+ L+E+I TVH    +IIR++   KK+  I  D    QDLLSRF+     +  E +R
Sbjct: 244 GSEKRLREAIKTVHLSVSEIIRAK---KKSLDIGGDVSDKQDLLSRFLAAG--HGEEAVR 303

Query: 303 DIIISFILAGRDTTSSALTWFFWILSSRPHIQQKILTELETIRSRTSKQIGETYSFDELR 362
           D +ISFI+AGRDTTS+A+TW FW+LS    ++ KIL EL   R++ S  +G    F++LR
Sbjct: 304 DSVISFIMAGRDTTSAAMTWLFWLLSQNDDVETKILDEL---RNKGSLGLG----FEDLR 363

Query: 363 DMHYLQAALSETLRLYPPVPVDTKACRNDDVLPDGTFIGKSWFVTYHTYAMGRMESIWGK 422
           +M Y +A L E +RLYPPV  D+K   NDD+LPDGT + K   VTY  Y MGRME +WGK
Sbjct: 364 EMSYTKACLCEAMRLYPPVAWDSKHAANDDILPDGTPLKKGDKVTYFPYGMGRMEKVWGK 423

Query: 423 NYGEFLPERWLE-------NGVCRTESPFRFPVFHAGPRMCLGKDMAYIQMKSIAAAVME 482
           ++ EF P RW E         V ++ S F+FPVF AGPR+C+GK+MA+ QMK +  +V+ 
Sbjct: 424 DWDEFKPNRWFEEEPSYGTKPVLKSVSSFKFPVFQAGPRVCIGKEMAFTQMKYVVGSVLS 483

Query: 483 RFEVGMAEKEKSPKHLLSLTLRMENGLQVIVKKRD 504
           RF++ +      P  +  LT  M  GL+V +K+R+
Sbjct: 484 RFKI-IPVCNNRPVFVPLLTAHMAGGLKVKIKRRE 503