Spg010157 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg010157
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Pectin lyase-like superfamily protein
Location	scaffold3: 23827783 .. 23829585 (+)
RNA-Seq Expression	Spg010157
Synteny	Spg010157

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTCTTCATACAAGTTTATGTCTTCTCTTACTATTAGTCATTATCTTCTTTAACTCAGCCCTTGCCACTCAATTGACATTTAGTGTTGTCAATTCTGGAGCCAAACCTGATGCTAAAATCGACTCATCCAAGGCCTTCCAATTAGCATGGGAACGTGCTTGTAGCTCTTCTCAAGCAGCAACTATTTACGTACCGGAGGGCAAATTCTATCTCCATAGTGTAACTTTTAAAGGGCCATGCAACAACAATGCAATCACCATACGCATTGATGGCACTCTCGTGGCTCCGTCAGACTTCCATGTTACCGACCCTCAAAATTGGATCAAATTTCATCTTGTAGATGGTGTTACTATCCTAGGTGGCGTTCTTGATGGACAAGGAACTGGGTTGTGGGATTGCAAACATTTTGGCAAGAATTGTCCTCGTGGAGCCACGGTATACAAATCTAACTTTATATATACTACTATTTTAATTAACAATATGTTTTCCTACTAAAATTCATTTATTACAAGATAAGGTTAATTATGTATCAAATTGTTTCTATTGAATGTAGTATGTATGTATGCGGTATAATGTAAGATAAACACCTTTCAGTTTTTTAAAACAACCATGATATCTACTATTTTTTTTAAAACAATTTGGAATGGTTTTGAAACGTTTTAATATTTATTTTATGTGTATTAATAGCTCAATTTAATTTATTAAATGTTATAAATAACAATAAATAAAGGATAAATAAAATATTAACCAAAAAAACTTATTACACATCCTCTCAATTTGATATACTTCCTTTTCCACGATACTAATTTTGCTCTCTAATTTCAAAACAACTTTTGTTTTGGACATGTGCAGTCACTGAAATTTACCAATTCACAAAACATAATGATAAGTGGATTAACCTCACTAGATAGTCAACTTTTCCACATAGTAGTGAATGAGTGTCGAAATGTGAAATTGCAAGGACTAAAGGTCTCGGCCGCTGGCAACAGTCCAAACACCGATGGCATTCACATAGGGTTGTCAACTTATGTCACCATCCTCAACTCCAATATCAGCACTGGCGATGACTGTGTCTCGATAGGCCCTGGCACCTCCAATTTGTGGATTGAGAATGTTGCTTGTGGACCTGGTCATGGAATTAGGTACTTCTTAAATTTTTATCAAGTGTTTGGGTTCTTTTTTGGATTCCTAATGAGAAATAGATGGTGTGTGTTTAATTGGTTGGTGCAGCATTGGGAGTTTAGGAAGAAACTTGAAGGAGGATGGAGTTCAGAACGTGACAGTGAAATCTTCTAGATTTACCAACACTCAAAATGGGGTCAGAATAAAAAGTAGGGGAATATCAAGTAGTGGGTTTGCAAGAAACATTCGTTTTCAACATCTCATAATGGATAACGTTAGGTATCCTATCATCATCGATCAAAATTACTGCCCCCACAATAAAGATTGTCCTGGACAGGTGCATATTGATATCTACACAACTATATACACACACACACACACACAAATATATATATATATATATATATATATAAAAGGATAGATCTAAACTTTTATATTTTATTTCTTCCCATTGAATAGGCTTCTGGAATTAAAGTTAGCGATGTGACATATCAAGACATCCATGGGACATCGGCCAAACAGGTCACCGTGAAATTTGATTGTAGCCCAAAGTTTCCTTGCACGGGGATAAAGTTGGAAGATGTAAAGCTAACTTACAAGAATGGAGTAGCCAAAGCTTCGTGTAGCCATGCTCGAGGAACTGTTGTTGGCTTGGTTCAACCAATGAGTTGTTTTTAG

mRNA sequence

ATGGCTCTTCATACAAGTTTATGTCTTCTCTTACTATTAGTCATTATCTTCTTTAACTCAGCCCTTGCCACTCAATTGACATTTAGTGTTGTCAATTCTGGAGCCAAACCTGATGCTAAAATCGACTCATCCAAGGCCTTCCAATTAGCATGGGAACGTGCTTGTAGCTCTTCTCAAGCAGCAACTATTTACGTACCGGAGGGCAAATTCTATCTCCATAGTGTAACTTTTAAAGGGCCATGCAACAACAATGCAATCACCATACGCATTGATGGCACTCTCGTGGCTCCGTCAGACTTCCATGTTACCGACCCTCAAAATTGGATCAAATTTCATCTTGTAGATGGTGTTACTATCCTAGGTGGCGTTCTTGATGGACAAGGAACTGGGTTGTGGGATTGCAAACATTTTGGCAAGAATTGTCCTCGTGGAGCCACGTCACTGAAATTTACCAATTCACAAAACATAATGATAAGTGGATTAACCTCACTAGATAGTCAACTTTTCCACATAGTAGTGAATGAGTGTCGAAATGTGAAATTGCAAGGACTAAAGGTCTCGGCCGCTGGCAACAGTCCAAACACCGATGGCATTCACATAGGGTTGTCAACTTATGTCACCATCCTCAACTCCAATATCAGCACTGGCGATGACTGTGTCTCGATAGGCCCTGGCACCTCCAATTTGTGGATTGAGAATGTTGCTTGTGGACCTGGTCATGGAATTAGCATTGGGAGTTTAGGAAGAAACTTGAAGGAGGATGGAGTTCAGAACGTCACCGTGAAATTTGATTGTAGCCCAAAGTTTCCTTGCACGGGGATAAAGTTGGAAGATGTAAAGCTAACTTACAAGAATGGAGTAGCCAAAGCTTCGTGTAGCCATGCTCGAGGAACTGTTGTTGGCTTGGTTCAACCAATGAGTTGTTTTTAG

Coding sequence (CDS)

ATGGCTCTTCATACAAGTTTATGTCTTCTCTTACTATTAGTCATTATCTTCTTTAACTCAGCCCTTGCCACTCAATTGACATTTAGTGTTGTCAATTCTGGAGCCAAACCTGATGCTAAAATCGACTCATCCAAGGCCTTCCAATTAGCATGGGAACGTGCTTGTAGCTCTTCTCAAGCAGCAACTATTTACGTACCGGAGGGCAAATTCTATCTCCATAGTGTAACTTTTAAAGGGCCATGCAACAACAATGCAATCACCATACGCATTGATGGCACTCTCGTGGCTCCGTCAGACTTCCATGTTACCGACCCTCAAAATTGGATCAAATTTCATCTTGTAGATGGTGTTACTATCCTAGGTGGCGTTCTTGATGGACAAGGAACTGGGTTGTGGGATTGCAAACATTTTGGCAAGAATTGTCCTCGTGGAGCCACGTCACTGAAATTTACCAATTCACAAAACATAATGATAAGTGGATTAACCTCACTAGATAGTCAACTTTTCCACATAGTAGTGAATGAGTGTCGAAATGTGAAATTGCAAGGACTAAAGGTCTCGGCCGCTGGCAACAGTCCAAACACCGATGGCATTCACATAGGGTTGTCAACTTATGTCACCATCCTCAACTCCAATATCAGCACTGGCGATGACTGTGTCTCGATAGGCCCTGGCACCTCCAATTTGTGGATTGAGAATGTTGCTTGTGGACCTGGTCATGGAATTAGCATTGGGAGTTTAGGAAGAAACTTGAAGGAGGATGGAGTTCAGAACGTCACCGTGAAATTTGATTGTAGCCCAAAGTTTCCTTGCACGGGGATAAAGTTGGAAGATGTAAAGCTAACTTACAAGAATGGAGTAGCCAAAGCTTCGTGTAGCCATGCTCGAGGAACTGTTGTTGGCTTGGTTCAACCAATGAGTTGTTTTTAG

Protein sequence

MALHTSLCLLLLLVIIFFNSALATQLTFSVVNSGAKPDAKIDSSKAFQLAWERACSSSQAATIYVPEGKFYLHSVTFKGPCNNNAITIRIDGTLVAPSDFHVTDPQNWIKFHLVDGVTILGGVLDGQGTGLWDCKHFGKNCPRGATSLKFTNSQNIMISGLTSLDSQLFHIVVNECRNVKLQGLKVSAAGNSPNTDGIHIGLSTYVTILNSNISTGDDCVSIGPGTSNLWIENVACGPGHGISIGSLGRNLKEDGVQNVTVKFDCSPKFPCTGIKLEDVKLTYKNGVAKASCSHARGTVVGLVQPMSCF

Homology

[Close]

BLAST of Spg010157 vs. NCBI nr
Match: XP_022953045.1 (polygalacturonase-like [Cucurbita moschata])

HSP 1 Score: 440.3 bits (1131), Expect = 1.4e-119
Identity = 230/380 (60.53%), Postives = 257/380 (67.63%), Query Frame = 0


Query: 5   TSLCLLLLLVIIFFNSALATQLTFSVVNSGAKPDAKIDSSKAFQLAWERACSSSQAATIY 64
           T+  LL LL+IIFFNS  A   TF+VV+ GAKPDAK DSSKAFQ AW RAC+SS +A+IY
Sbjct: 28  TTSFLLSLLLIIFFNSPFAASETFNVVSFGAKPDAKTDSSKAFQAAWARACTSSTSASIY 87

Query: 65  VPEGKFYLHSVTFKGPCNNNAITIRIDGTLVAPSDFHV-TDPQNWIKFHLVDGVTILGGV 124
           VP G+FYL   TF GPC N+AI I IDGTLVAPSDFHV  +  NWI FHLV+GV+I GG 
Sbjct: 88  VPNGRFYLRHATFNGPCRNDAIDIHIDGTLVAPSDFHVNANSPNWIIFHLVNGVSIFGGT 147

Query: 125 LDGQGTGLWDCKHFGKNCPRGATSLKFTNSQNIMISGLTSLDSQLFHIVVNECRNVKLQG 184
           LDGQG GLW CK   K CPRGATSL+FT+S+NIMISGLTSL+SQ+FHIVVNEC NVK++G
Sbjct: 148 LDGQGAGLWACKRSAKTCPRGATSLEFTDSKNIMISGLTSLNSQIFHIVVNECHNVKMEG 207

Query: 185 LKVSAAGNSPNTDGIHIGLSTYVTILNSNISTGDDCVSIGPGTSNLWIENVACGPGHGIS 244
           LKVSA+GNSPNTDGIH+GLS++VTILNS+ISTGDDC+SIGPGTSNLWIENVACGPGHGIS
Sbjct: 208 LKVSASGNSPNTDGIHVGLSSHVTILNSDISTGDDCISIGPGTSNLWIENVACGPGHGIS 267

Query: 245 IGSLGRNLKEDGVQNVTVK----------------------------------------- 302
           IGSLG+ LKEDGVQNVTVK                                         
Sbjct: 268 IGSLGKALKEDGVQNVTVKSCRFMNTQNGVRIKSWGRPSSGFARNIRFQHLKMTNVKNPI 327

[Close]

BLAST of Spg010157 vs. NCBI nr
Match: XP_023511587.1 (polygalacturonase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 437.6 bits (1124), Expect = 9.1e-119
Identity = 228/380 (60.00%), Postives = 256/380 (67.37%), Query Frame = 0


Query: 5   TSLCLLLLLVIIFFNSALATQLTFSVVNSGAKPDAKIDSSKAFQLAWERACSSSQAATIY 64
           T+  LL LL+IIFFNS  A   TF+VVN GAKPDAK DSSKAFQ AW RAC+SS +A+IY
Sbjct: 5   TTSFLLSLLLIIFFNSPFAASETFNVVNFGAKPDAKTDSSKAFQAAWARACTSSTSASIY 64

Query: 65  VPEGKFYLHSVTFKGPCNNNAITIRIDGTLVAPSDFHV-TDPQNWIKFHLVDGVTILGGV 124
           +P G+FYL   TF GPC N+AI I IDGTLVAPSDFHV  +  NWI FHLV+GV+I GG 
Sbjct: 65  IPNGRFYLRHATFNGPCINHAIDIHIDGTLVAPSDFHVNANSPNWIIFHLVNGVSIFGGT 124

Query: 125 LDGQGTGLWDCKHFGKNCPRGATSLKFTNSQNIMISGLTSLDSQLFHIVVNECRNVKLQG 184
           LDGQG GLW CK   K CPRGATSL+FTNS+NIMISGLTSL+S +FHIVVNEC +VK++G
Sbjct: 125 LDGQGAGLWACKRTAKTCPRGATSLEFTNSKNIMISGLTSLNSHIFHIVVNECHDVKMEG 184

Query: 185 LKVSAAGNSPNTDGIHIGLSTYVTILNSNISTGDDCVSIGPGTSNLWIENVACGPGHGIS 244
           LKVSA+GNSPNTDGIH+GLS++VTILNS+ISTGDDC+SIGPGTSNLWIENVACGPGHGIS
Sbjct: 185 LKVSASGNSPNTDGIHVGLSSHVTILNSDISTGDDCISIGPGTSNLWIENVACGPGHGIS 244

Query: 245 IGSLGRNLKEDGVQNVTVK----------------------------------------- 302
           IGSLG+ LKEDGVQNVTVK                                         
Sbjct: 245 IGSLGKALKEDGVQNVTVKSCRFMNTQNGVRIKSWGRPSSGFARNIRFQHLKMTNVKNPI 304

[Close]

BLAST of Spg010157 vs. NCBI nr
Match: XP_022953043.1 (polygalacturonase-like [Cucurbita moschata])

HSP 1 Score: 437.2 bits (1123), Expect = 1.2e-118
Identity = 229/380 (60.26%), Postives = 256/380 (67.37%), Query Frame = 0


Query: 5   TSLCLLLLLVIIFFNSALATQLTFSVVNSGAKPDAKIDSSKAFQLAWERACSSSQAATIY 64
           T+  LL LL+IIFFNS  A   TF+VV+ GAKPDAK DSSKAFQ AW RAC+SS +A+IY
Sbjct: 5   TTSFLLSLLLIIFFNSPFAASETFNVVSFGAKPDAKTDSSKAFQAAWARACTSSTSASIY 64

Query: 65  VPEGKFYLHSVTFKGPCNNNAITIRIDGTLVAPSDFHV-TDPQNWIKFHLVDGVTILGGV 124
           VP G+FYL   TF GPC N+AI I IDGTLVAPSDFHV  +  NWI FHLV+GV+I GG 
Sbjct: 65  VPNGRFYLRHATFNGPCRNDAIDIHIDGTLVAPSDFHVNANSPNWIIFHLVNGVSIFGGT 124

Query: 125 LDGQGTGLWDCKHFGKNCPRGATSLKFTNSQNIMISGLTSLDSQLFHIVVNECRNVKLQG 184
           LDGQG GLW CK   K CPRGATSL+FT+S+NIMISGLTSL+SQ+FHIVVNEC NVK++G
Sbjct: 125 LDGQGAGLWACKRSAKTCPRGATSLEFTDSKNIMISGLTSLNSQIFHIVVNECHNVKMEG 184

Query: 185 LKVSAAGNSPNTDGIHIGLSTYVTILNSNISTGDDCVSIGPGTSNLWIENVACGPGHGIS 244
           LKVSA+GNSPNTDGIH+ LS++VTILNS+ISTGDDC+SIGPGTSNLWIENVACGPGHGIS
Sbjct: 185 LKVSASGNSPNTDGIHVELSSHVTILNSDISTGDDCISIGPGTSNLWIENVACGPGHGIS 244

Query: 245 IGSLGRNLKEDGVQNVTVK----------------------------------------- 302
           IGSLG+ LKEDGVQNVTVK                                         
Sbjct: 245 IGSLGKALKEDGVQNVTVKSCRFMNTQNGVRIKSWGRPSSGFARNIRFQHLKMTNVKNPI 304

[Close]

BLAST of Spg010157 vs. NCBI nr
Match: XP_023511588.1 (polygalacturonase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 435.6 bits (1119), Expect = 3.5e-118
Identity = 228/380 (60.00%), Postives = 255/380 (67.11%), Query Frame = 0


Query: 5   TSLCLLLLLVIIFFNSALATQLTFSVVNSGAKPDAKIDSSKAFQLAWERACSSSQAATIY 64
           T+  LL LL+IIFFNS  A   TF+VVN GAKPDAK DSSKAFQ AW RAC+SS +A+IY
Sbjct: 5   TTSFLLSLLLIIFFNSPFAASETFNVVNFGAKPDAKTDSSKAFQAAWARACTSSTSASIY 64

Query: 65  VPEGKFYLHSVTFKGPCNNNAITIRIDGTLVAPSDFHV-TDPQNWIKFHLVDGVTILGGV 124
           VP G+FYL   TF GPC N+AI I IDGTLVAPSDFHV  +  NWI FHLV+GV+I GG 
Sbjct: 65  VPNGRFYLRHATFNGPCINDAIDIHIDGTLVAPSDFHVNANSPNWIIFHLVNGVSIFGGT 124

Query: 125 LDGQGTGLWDCKHFGKNCPRGATSLKFTNSQNIMISGLTSLDSQLFHIVVNECRNVKLQG 184
           LDGQG GLW CK   K CPRGATSL+FTNS+NIMISGLTSL+S +FHIVVNEC +VK++G
Sbjct: 125 LDGQGAGLWACKRTAKTCPRGATSLEFTNSKNIMISGLTSLNSHIFHIVVNECHDVKMEG 184

Query: 185 LKVSAAGNSPNTDGIHIGLSTYVTILNSNISTGDDCVSIGPGTSNLWIENVACGPGHGIS 244
           LKVSA+GNSPNTDGIH+GLS++VTILNS+ISTGDDC+SIGPGTSNLWIENVACGPGHGIS
Sbjct: 185 LKVSASGNSPNTDGIHVGLSSHVTILNSDISTGDDCISIGPGTSNLWIENVACGPGHGIS 244

Query: 245 IGSLGRNLKEDGVQNVTVK----------------------------------------- 302
           IGSLG+ LKEDGVQNVTVK                                         
Sbjct: 245 IGSLGKALKEDGVQNVTVKSCRFMNTQNGVRIKSWGRPSSGFARNIRFQHLKMTNVKNPI 304

[Close]

BLAST of Spg010157 vs. NCBI nr
Match: XP_022971874.1 (polygalacturonase-like [Cucurbita maxima])

HSP 1 Score: 431.8 bits (1109), Expect = 5.0e-117
Identity = 224/380 (58.95%), Postives = 255/380 (67.11%), Query Frame = 0


Query: 5   TSLCLLLLLVIIFFNSALATQLTFSVVNSGAKPDAKIDSSKAFQLAWERACSSSQAATIY 64
           T+  LL L ++IFFNS  A   T +VVN GAKPDAK DSSKAFQ AW RAC+SS +++IY
Sbjct: 5   TTSFLLSLQLVIFFNSPFAASETLNVVNFGAKPDAKTDSSKAFQAAWARACTSSTSSSIY 64

Query: 65  VPEGKFYLHSVTFKGPCNNNAITIRIDGTLVAPSDFHV-TDPQNWIKFHLVDGVTILGGV 124
           VP G+FYL   TF GPC ++AI I IDGTLVAPSDFHV  +  NWI FHLV+GV+I GG 
Sbjct: 65  VPNGRFYLRHATFNGPCRSDAIDIHIDGTLVAPSDFHVNANSPNWIIFHLVNGVSIFGGT 124

Query: 125 LDGQGTGLWDCKHFGKNCPRGATSLKFTNSQNIMISGLTSLDSQLFHIVVNECRNVKLQG 184
           LDGQG GLW CK   K CPRGATSL+FT+S+NI+ISGLTSL+SQ+FHIVVNEC NVK++G
Sbjct: 125 LDGQGAGLWACKRSAKTCPRGATSLEFTDSKNILISGLTSLNSQIFHIVVNECHNVKMEG 184

Query: 185 LKVSAAGNSPNTDGIHIGLSTYVTILNSNISTGDDCVSIGPGTSNLWIENVACGPGHGIS 244
           LKVSA+GNSPNTDGIH+GLS++VTILNS+ISTGDDC+SIGPGTSNLWIENVACGPGHGIS
Sbjct: 185 LKVSASGNSPNTDGIHVGLSSHVTILNSDISTGDDCISIGPGTSNLWIENVACGPGHGIS 244

Query: 245 IGSLGRNLKEDGVQNVTVK----------------------------------------- 302
           IGSLG+ LKEDGVQNVTVK                                         
Sbjct: 245 IGSLGKALKEDGVQNVTVKSCRFTNTQNGVRIKSWGRPSSGFARNIRFQHLKMTNVKNPI 304

[Close]

BLAST of Spg010157 vs. ExPASy Swiss-Prot
Match: P48979 (Polygalacturonase OS=Prunus persica OX=3760 PE=2 SV=1)

HSP 1 Score: 373.6 bits (958), Expect = 2.1e-102
Identity = 201/392 (51.28%), Postives = 242/392 (61.73%), Query Frame = 0


Query: 1   MALHTSLCLLLLLVIIFFNSALATQLTFSVVNSGAKPDAKIDSSKAFQLAWERACSSSQA 60
           MA   SL  L L+ +   NSA+A+ LT++V + GAK D K DS+KAF  AW +AC+S   
Sbjct: 1   MANRRSLFSLSLIFVFMINSAIASPLTYNVASLGAKADGKTDSTKAFLSAWAKACASMNP 60

Query: 61  ATIYVPEGKFYLHSVTFKGPCNNNAITIRIDGTLVAPSDFHVT-DPQNWIKFHLVDGVTI 120
             IYVP G F+L  V F GPC NNAIT RI GTLVAPSD+ V  +  NWI FH V+GVTI
Sbjct: 61  GVIYVPAGTFFLRDVVFSGPCKNNAITFRIAGTLVAPSDYRVIGNAANWIFFHHVNGVTI 120

Query: 121 LGGVLDGQGTGLWDCKH-FGKNCPRGATSLKFTNSQNIMISGLTSLDSQLFHIVVNECRN 180
            GG+LDGQGT LW CK   G++CP GAT+L F++S NI++SGL SL+SQ+FHIV+N+ +N
Sbjct: 121 SGGILDGQGTALWACKACHGESCPSGATTLGFSDSNNIVVSGLASLNSQMFHIVINDFQN 180

Query: 181 VKLQGLKVSAAGNSPNTDGIHIGLSTYVTILNSNISTGDDCVSIGPGTSNLWIENVACGP 240
           V++QG++VS +GNSPNTDGIH+ +S+ VTILNS I+TGDDCVSIGPGTSNLWIE VACGP
Sbjct: 181 VQMQGVRVSRSGNSPNTDGIHVQMSSGVTILNSKIATGDDCVSIGPGTSNLWIEGVACGP 240

Query: 241 GHGISIGSLGRNLKEDGVQNVT-------------------------------------- 300
           GHGISIGSLG+  +E GVQNVT                                      
Sbjct: 241 GHGISIGSLGKEQEEAGVQNVTVKTVTFSGTQNGLRIKSWGRPSTGFARNILFQHATMVN 300

Query: 301 --------------------------------------------VKFDCSPKFPCTGIKL 309
                                                       VKFDCSPK PC  IKL
Sbjct: 301 VENPIVIDQHYCPDNKGCPGQVSGVQISDVTYEDIHGTSATEVAVKFDCSPKHPCREIKL 360

[Close]

BLAST of Spg010157 vs. ExPASy Swiss-Prot
Match: O22818 (Probable polygalacturonase At2g43860 OS=Arabidopsis thaliana OX=3702 GN=At2g43860 PE=2 SV=1)

HSP 1 Score: 267.7 bits (683), Expect = 1.6e-70
Identity = 158/396 (39.90%), Postives = 210/396 (53.03%), Query Frame = 0


Query: 3   LHTSLCLLLLLVIIFFNSALATQL------TFSVVNSGAKPDAKIDSSKAFQLAWERACS 62
           ++ + CL+L L +I  +  L + L      T +V++ GAKPD   DS+KAF  AW+ AC+
Sbjct: 2   VYNTSCLILPLALIILDFTLISSLAHPIPSTLNVLSYGAKPDGSKDSTKAFLAAWDVACA 61

Query: 63  SSQAATIYVPEGKFYLHSVTFKG-PCNNNAITIRIDGTLVAPSDFH-VTDPQNWIKFHLV 122
           S+   TI VP+G+F + ++ F G  C    I+IRI G++VAP DF  +   ++WI F  V
Sbjct: 62  SANPTTIIVPKGRFLVGNLVFHGNECKQAPISIRIAGSIVAPEDFRIIASSKHWIWFEDV 121

Query: 123 DGVTILGGVLDGQGTGLWDCK-HFGKNCPRGATSLKFTNSQNIMISGLTSLDSQLFHIVV 182
             V+I GG+LD QGT LW CK + G NCP GA SL F+ S NI ISGLTS++SQ FHIV+
Sbjct: 122 TDVSIYGGILDAQGTSLWKCKNNGGHNCPTGAKSLVFSGSNNIKISGLTSINSQKFHIVI 181

Query: 183 NECRNVKLQGLKVSAAGNSPNTDGIHIGLSTYVTILNSNISTGDDCVSIGPGTSNLWIEN 242
           +   NV + G+KVSA  NSPNTDGIH+  S  V I NS I TGDDC+SIGPG++N++I+ 
Sbjct: 182 DNSNNVNIDGVKVSADENSPNTDGIHVESSHSVHITNSRIGTGDDCISIGPGSTNVFIQT 241

Query: 243 VACGPGHGISIGSLGRNLKEDGVQNVTVK------------------------------- 302
           + CGPGHGISIGSLGR  +E GV NVTV                                
Sbjct: 242 IRCGPGHGISIGSLGRAEEEQGVDNVTVSNVDFMGTNNGVRIKTWGKDSNSFARNIVFQH 301

Query: 303 --------------------------------------------------FDCSPKFPCT 309
                                                              DCS + PCT
Sbjct: 302 INMKMVKNPIIIDQHYCLHKPCPKQESGVKVSNVRYEDIHGTSNTEVAVLLDCSKEKPCT 361

[Close]

BLAST of Spg010157 vs. ExPASy Swiss-Prot
Match: Q6H9K0 (Exopolygalacturonase (Fragment) OS=Platanus acerifolia OX=140101 GN=plaa2 PE=1 SV=1)

HSP 1 Score: 213.8 bits (543), Expect = 2.8e-54
Identity = 109/253 (43.08%), Postives = 158/253 (62.45%), Query Frame = 0


Query: 28  FSVVNSGAKPDAKIDSSKAFQLAWERACSSSQAATIYVPEGKFYLHSVTFKGPCNNNAIT 87
           F+V + GAK     D S+A   AW+ AC+S   +T+ +P+G + +  V  +GPC  + I 
Sbjct: 10  FNVNDYGAKGAG--DISQAVMKAWKAACASQGPSTVLIPKGNYNMGEVAMQGPCKGSKIG 69

Query: 88  IRIDGTLVAPSDFHVTDPQNWIKFHLVDGVTILG-GVLDGQGTGLWDCKHFGK--NCPRG 147
            +IDG + AP+D        W+ F+ +DG+T+ G G LDGQG   W   +  K  NC   
Sbjct: 70  FQIDGVVKAPADPSKFKSDGWVSFYRIDGLTVSGTGTLDGQGQTAWAKNNCDKNPNCKHA 129

Query: 148 ATSLKFTNSQNIMISGLTSLDSQLFHIVVNECRNVKLQGLKVSAAGNSPNTDGIHIGLST 207
           A +L+F   ++ M+  +TSL+S++FHI V EC ++  Q + V+A G S NTDGIH+G+S 
Sbjct: 130 AMNLRFDFLKHAMVRDITSLNSKMFHINVLECEDITFQHVTVTAPGTSINTDGIHVGISK 189

Query: 208 YVTILNSNISTGDDCVSIGPGTSNLWIENVACGPGHGISIGSLGRNLKEDGVQNVTVKFD 267
            VTI N+ I+TGDDC+SIGPG+ N+ I  V CGPGHGISIGSLGR   E  V+ +TVK  
Sbjct: 190 GVTITNTKIATGDDCISIGPGSQNVTITQVNCGPGHGISIGSLGRYNNEKEVRGITVK-G 249

Query: 268 CSPKFPCTGIKLE 278
           C+      G++++
Sbjct: 250 CTFSGTMNGVRVK 259

[Close]

BLAST of Spg010157 vs. ExPASy Swiss-Prot
Match: P35338 (Exopolygalacturonase OS=Zea mays OX=4577 GN=PG9 PE=2 SV=1)

HSP 1 Score: 204.5 bits (519), Expect = 1.7e-51
Identity = 109/255 (42.75%), Postives = 156/255 (61.18%), Query Frame = 0


Query: 27  TFSVVNSGAKPDAKIDSSKAFQLAWERACSSSQAATIYVPEGKFYLHSVTFKGPCNNNAI 86
           +F +   GA  + K DS+KA Q AW  AC  +   TI +P+G F +  + F GPC  + +
Sbjct: 40  SFDITKLGASGNGKTDSTKAVQEAWASACGGTGKQTILIPKGDFLVGQLNFTGPCKGD-V 99

Query: 87  TIRIDGTLVAPSDF-HVTDPQNWIKFHLVDGVTILG-GVLDGQGTGLWDCKHFGK--NCP 146
           TI++DG L+A +D     +  NWI+   VD + I G G LDGQG  +W      K  +C 
Sbjct: 100 TIQVDGNLLATTDLSQYKEHGNWIEILRVDNLVITGKGNLDGQGPAVWSKNSCTKKYDCK 159

Query: 147 RGATSLKFTNSQNIMISGLTSLDSQLFHIVVNECRNVKLQGLKVSAAGNSPNTDGIHIGL 206
               SL      N  +SG+T L+S+ FH+ + +C+N+ ++ + V+A G+SPNTDGIH+G 
Sbjct: 160 ILPNSLVMDFVNNGEVSGVTLLNSKFFHMNMYQCKNMLIKDVTVTAPGDSPNTDGIHMGD 219

Query: 207 STYVTILNSNISTGDDCVSIGPGTSNLWIENVACGPGHGISIGSLGRNLKEDGVQNVTVK 266
           S+ +TI N+ I  GDDC+SIGPGTS + I  V CGPGHGISIGSLGR   E  V ++ VK
Sbjct: 220 SSGITITNTVIGVGDDCISIGPGTSKVNITGVTCGPGHGISIGSLGRYKDEKDVTDINVK 279

Query: 267 FDCSPKFPCTGIKLE 278
            DC+ K    G++++
Sbjct: 280 -DCTLKKTMFGVRIK 292

[Close]

BLAST of Spg010157 vs. ExPASy Swiss-Prot
Match: P49063 (Exopolygalacturonase clone GBGA483 OS=Arabidopsis thaliana OX=3702 GN=At3g07850 PE=2 SV=2)

HSP 1 Score: 204.1 bits (518), Expect = 2.2e-51
Identity = 110/251 (43.82%), Postives = 152/251 (60.56%), Query Frame = 0


Query: 33  SGAKPDAKIDSSKAFQLAWERACSSSQAATIYVPEGKFYLHSVTFKGPCNNNAITIRIDG 92
           SGAK D+K D S AF  AW+ AC++   +TI VP+G++ + S+ FKGPC    +T+ ++G
Sbjct: 75  SGAKGDSKTDDSAAFAAAWKEACAA--GSTITVPKGEYMVESLEFKGPC-KGPVTLELNG 134

Query: 93  TLVAPSDFHVTDPQ-NWIKFHLVDGVTILG--GVLDGQGTGLW---DCKHFGKNCPRGAT 152
              AP+    T P   WI F  +   T+ G   + DGQG+  W   DC   GK C     
Sbjct: 135 NFKAPATVKTTKPHAGWIDFENIADFTLNGNKAIFDGQGSLAWKANDCAKTGK-CNSLPI 194

Query: 153 SLKFTNSQNIMISGLTSLDSQLFHIVVNECRNVKLQGLKVSAAGNSPNTDGIHIGLSTYV 212
           +++FT   N  I+ +TS +S+LFH+ +  C+N+ L  + + A   S NTDGIHIG S  V
Sbjct: 195 NIRFTGLTNSKINSITSTNSKLFHMNILNCKNITLSDIGIDAPPESLNTDGIHIGRSNGV 254

Query: 213 TILNSNISTGDDCVSIGPGTSNLWIENVACGPGHGISIGSLGRNLKEDGVQNVTVKFDCS 272
            ++ + I TGDDCVSIG GT NL +ENV CGPGHGISIGSLGR   E  V+ VTV+  C 
Sbjct: 255 NLIGAKIKTGDDCVSIGDGTENLIVENVECGPGHGISIGSLGRYPNEQPVKGVTVR-KCL 314

Query: 273 PKFPCTGIKLE 278
            K    G++++
Sbjct: 315 IKNTDNGVRIK 320

[Close]

BLAST of Spg010157 vs. ExPASy TrEMBL
Match: A0A6J1GNK7 (polygalacturonase-like OS=Cucurbita moschata OX=3662 GN=LOC111455565 PE=3 SV=1)

HSP 1 Score: 440.3 bits (1131), Expect = 6.8e-120
Identity = 230/380 (60.53%), Postives = 257/380 (67.63%), Query Frame = 0


Query: 5   TSLCLLLLLVIIFFNSALATQLTFSVVNSGAKPDAKIDSSKAFQLAWERACSSSQAATIY 64
           T+  LL LL+IIFFNS  A   TF+VV+ GAKPDAK DSSKAFQ AW RAC+SS +A+IY
Sbjct: 28  TTSFLLSLLLIIFFNSPFAASETFNVVSFGAKPDAKTDSSKAFQAAWARACTSSTSASIY 87

Query: 65  VPEGKFYLHSVTFKGPCNNNAITIRIDGTLVAPSDFHV-TDPQNWIKFHLVDGVTILGGV 124
           VP G+FYL   TF GPC N+AI I IDGTLVAPSDFHV  +  NWI FHLV+GV+I GG 
Sbjct: 88  VPNGRFYLRHATFNGPCRNDAIDIHIDGTLVAPSDFHVNANSPNWIIFHLVNGVSIFGGT 147

Query: 125 LDGQGTGLWDCKHFGKNCPRGATSLKFTNSQNIMISGLTSLDSQLFHIVVNECRNVKLQG 184
           LDGQG GLW CK   K CPRGATSL+FT+S+NIMISGLTSL+SQ+FHIVVNEC NVK++G
Sbjct: 148 LDGQGAGLWACKRSAKTCPRGATSLEFTDSKNIMISGLTSLNSQIFHIVVNECHNVKMEG 207

Query: 185 LKVSAAGNSPNTDGIHIGLSTYVTILNSNISTGDDCVSIGPGTSNLWIENVACGPGHGIS 244
           LKVSA+GNSPNTDGIH+GLS++VTILNS+ISTGDDC+SIGPGTSNLWIENVACGPGHGIS
Sbjct: 208 LKVSASGNSPNTDGIHVGLSSHVTILNSDISTGDDCISIGPGTSNLWIENVACGPGHGIS 267

Query: 245 IGSLGRNLKEDGVQNVTVK----------------------------------------- 302
           IGSLG+ LKEDGVQNVTVK                                         
Sbjct: 268 IGSLGKALKEDGVQNVTVKSCRFMNTQNGVRIKSWGRPSSGFARNIRFQHLKMTNVKNPI 327

[Close]

BLAST of Spg010157 vs. ExPASy TrEMBL
Match: A0A6J1GMA1 (polygalacturonase-like OS=Cucurbita moschata OX=3662 GN=LOC111455564 PE=3 SV=1)

HSP 1 Score: 437.2 bits (1123), Expect = 5.8e-119
Identity = 229/380 (60.26%), Postives = 256/380 (67.37%), Query Frame = 0


Query: 5   TSLCLLLLLVIIFFNSALATQLTFSVVNSGAKPDAKIDSSKAFQLAWERACSSSQAATIY 64
           T+  LL LL+IIFFNS  A   TF+VV+ GAKPDAK DSSKAFQ AW RAC+SS +A+IY
Sbjct: 5   TTSFLLSLLLIIFFNSPFAASETFNVVSFGAKPDAKTDSSKAFQAAWARACTSSTSASIY 64

Query: 65  VPEGKFYLHSVTFKGPCNNNAITIRIDGTLVAPSDFHV-TDPQNWIKFHLVDGVTILGGV 124
           VP G+FYL   TF GPC N+AI I IDGTLVAPSDFHV  +  NWI FHLV+GV+I GG 
Sbjct: 65  VPNGRFYLRHATFNGPCRNDAIDIHIDGTLVAPSDFHVNANSPNWIIFHLVNGVSIFGGT 124

Query: 125 LDGQGTGLWDCKHFGKNCPRGATSLKFTNSQNIMISGLTSLDSQLFHIVVNECRNVKLQG 184
           LDGQG GLW CK   K CPRGATSL+FT+S+NIMISGLTSL+SQ+FHIVVNEC NVK++G
Sbjct: 125 LDGQGAGLWACKRSAKTCPRGATSLEFTDSKNIMISGLTSLNSQIFHIVVNECHNVKMEG 184

Query: 185 LKVSAAGNSPNTDGIHIGLSTYVTILNSNISTGDDCVSIGPGTSNLWIENVACGPGHGIS 244
           LKVSA+GNSPNTDGIH+ LS++VTILNS+ISTGDDC+SIGPGTSNLWIENVACGPGHGIS
Sbjct: 185 LKVSASGNSPNTDGIHVELSSHVTILNSDISTGDDCISIGPGTSNLWIENVACGPGHGIS 244

Query: 245 IGSLGRNLKEDGVQNVTVK----------------------------------------- 302
           IGSLG+ LKEDGVQNVTVK                                         
Sbjct: 245 IGSLGKALKEDGVQNVTVKSCRFMNTQNGVRIKSWGRPSSGFARNIRFQHLKMTNVKNPI 304

[Close]

BLAST of Spg010157 vs. ExPASy TrEMBL
Match: A0A6J1I9T6 (polygalacturonase-like OS=Cucurbita maxima OX=3661 GN=LOC111470567 PE=3 SV=1)

HSP 1 Score: 431.8 bits (1109), Expect = 2.4e-117
Identity = 224/380 (58.95%), Postives = 255/380 (67.11%), Query Frame = 0


Query: 5   TSLCLLLLLVIIFFNSALATQLTFSVVNSGAKPDAKIDSSKAFQLAWERACSSSQAATIY 64
           T+  LL L ++IFFNS  A   T +VVN GAKPDAK DSSKAFQ AW RAC+SS +++IY
Sbjct: 5   TTSFLLSLQLVIFFNSPFAASETLNVVNFGAKPDAKTDSSKAFQAAWARACTSSTSSSIY 64

Query: 65  VPEGKFYLHSVTFKGPCNNNAITIRIDGTLVAPSDFHV-TDPQNWIKFHLVDGVTILGGV 124
           VP G+FYL   TF GPC ++AI I IDGTLVAPSDFHV  +  NWI FHLV+GV+I GG 
Sbjct: 65  VPNGRFYLRHATFNGPCRSDAIDIHIDGTLVAPSDFHVNANSPNWIIFHLVNGVSIFGGT 124

Query: 125 LDGQGTGLWDCKHFGKNCPRGATSLKFTNSQNIMISGLTSLDSQLFHIVVNECRNVKLQG 184
           LDGQG GLW CK   K CPRGATSL+FT+S+NI+ISGLTSL+SQ+FHIVVNEC NVK++G
Sbjct: 125 LDGQGAGLWACKRSAKTCPRGATSLEFTDSKNILISGLTSLNSQIFHIVVNECHNVKMEG 184

Query: 185 LKVSAAGNSPNTDGIHIGLSTYVTILNSNISTGDDCVSIGPGTSNLWIENVACGPGHGIS 244
           LKVSA+GNSPNTDGIH+GLS++VTILNS+ISTGDDC+SIGPGTSNLWIENVACGPGHGIS
Sbjct: 185 LKVSASGNSPNTDGIHVGLSSHVTILNSDISTGDDCISIGPGTSNLWIENVACGPGHGIS 244

Query: 245 IGSLGRNLKEDGVQNVTVK----------------------------------------- 302
           IGSLG+ LKEDGVQNVTVK                                         
Sbjct: 245 IGSLGKALKEDGVQNVTVKSCRFTNTQNGVRIKSWGRPSSGFARNIRFQHLKMTNVKNPI 304

[Close]

BLAST of Spg010157 vs. ExPASy TrEMBL
Match: A0A6J1I881 (polygalacturonase-like OS=Cucurbita maxima OX=3661 GN=LOC111470566 PE=3 SV=1)

HSP 1 Score: 424.1 bits (1089), Expect = 5.0e-115
Identity = 220/380 (57.89%), Postives = 252/380 (66.32%), Query Frame = 0


Query: 5   TSLCLLLLLVIIFFNSALATQLTFSVVNSGAKPDAKIDSSKAFQLAWERACSSSQAATIY 64
           T+  LL L ++IFFNS  A   T +VVN GAKPDAK DSSKAFQ AW RAC+S  +A+IY
Sbjct: 5   TTSFLLSLQLVIFFNSPFAASETLNVVNFGAKPDAKTDSSKAFQAAWARACTSCTSASIY 64

Query: 65  VPEGKFYLHSVTFKGPCNNNAITIRIDGTLVAPSDFHV-TDPQNWIKFHLVDGVTILGGV 124
           VP G+FYL   TF GPC ++AI I IDGTLVAPSDFHV  +  NWI FHLV+GV+I GG 
Sbjct: 65  VPNGRFYLRHATFNGPCRSDAINIHIDGTLVAPSDFHVNANSPNWIIFHLVNGVSIFGGT 124

Query: 125 LDGQGTGLWDCKHFGKNCPRGATSLKFTNSQNIMISGLTSLDSQLFHIVVNECRNVKLQG 184
           LDGQG GLW CK   K CP GATSL+FT+S+NI+ISGLTSL+SQ+FHIVVNEC NVK++G
Sbjct: 125 LDGQGAGLWACKRSAKTCPLGATSLEFTDSKNILISGLTSLNSQIFHIVVNECHNVKMEG 184

Query: 185 LKVSAAGNSPNTDGIHIGLSTYVTILNSNISTGDDCVSIGPGTSNLWIENVACGPGHGIS 244
           LKV+A+GNSPNTDGIH+GLS++VTILNS+ISTGDDC+SIGPGTSNLWIENVACGPGHGIS
Sbjct: 185 LKVTASGNSPNTDGIHVGLSSHVTILNSDISTGDDCISIGPGTSNLWIENVACGPGHGIS 244

Query: 245 IGSLGRNLKEDGVQNVTVK----------------------------------------- 302
           IGSLG+ LKEDGVQNVTVK                                         
Sbjct: 245 IGSLGKALKEDGVQNVTVKSCRFTNTQNGVRIKSWGRPSSGFARNIRFQHLKMTNVKNPI 304

[Close]

BLAST of Spg010157 vs. ExPASy TrEMBL
Match: A0A1S3BJI1 (polygalacturonase-like OS=Cucumis melo OX=3656 GN=LOC103490378 PE=3 SV=1)

HSP 1 Score: 423.7 bits (1088), Expect = 6.6e-115
Identity = 225/398 (56.53%), Postives = 265/398 (66.58%), Query Frame = 0


Query: 1   MALHTSLCLLL---LLVIIFFN--SALATQLTFSVVNSGAKP-DAKIDSSKAFQLAWERA 60
           MALH     LL   L V++ FN  +A  T LTF+VV+ G KP +A+ DSSKA QLAW  A
Sbjct: 1   MALHAITSFLLFSTLTVLMLFNNLAAFPTPLTFNVVDFGTKPNNAETDSSKALQLAWMHA 60

Query: 61  CSSSQAATIYVPEGKFYLHSVTFKGPCNNNAITIRIDGTLVAPSDFHVT-DPQNWIKFHL 120
           CSSS+ ATIYVP+ KFY+ S TF GPC NN ITIRIDG L+APSDFH+T + +NWI FH 
Sbjct: 61  CSSSKPATIYVPKAKFYVRSATFNGPCKNNHITIRIDGALMAPSDFHLTSNSENWILFHN 120

Query: 121 VDGVTILGGVLDGQGTGLWDCKHFGKNCPRGATSLKFTNSQNIMISGLTSLDSQLFHIVV 180
           V+GVT+LGG+LDGQGT LW CK+    CPRG TSL+FTNSQNI+++GLT+L+SQL+HIVV
Sbjct: 121 VNGVTVLGGILDGQGTQLWACKNSANTCPRGTTSLEFTNSQNILVNGLTALNSQLYHIVV 180

Query: 181 NECRNVKLQGLKVSAAGNSPNTDGIHIGLSTYVTILNSNISTGDDCVSIGPGTSNLWIEN 240
           N+CRNVK+QGLKVSAAGNSPNTDGIH+G S++VTILN+NI TGDDC+SIGPG+SNLWIEN
Sbjct: 181 NQCRNVKMQGLKVSAAGNSPNTDGIHVGDSSHVTILNTNIRTGDDCISIGPGSSNLWIEN 240

Query: 241 VACGPGHGISIGSLGRNLKEDGVQNVTV-------------------------------- 300
           +ACGPGHGISIGSL ++ KE GVQNVTV                                
Sbjct: 241 IACGPGHGISIGSLAKDFKEKGVQNVTVKSCKFMDTQNGVRIKSWGRQSIGFARNIRFQH 300

Query: 301 --------------------------------------------------KFDCSPKFPC 310
                                                             KFDCSPKFPC
Sbjct: 301 LTMTNVQNPIIIDQNYCPHNQGCPGQSSGVAVSDVKYTDIRGTSATPVAIKFDCSPKFPC 360

[Close]

BLAST of Spg010157 vs. TAIR 10
Match: AT2G43870.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 345.1 bits (884), Expect = 5.7e-95
Identity = 182/379 (48.02%), Postives = 230/379 (60.69%), Query Frame = 0


Query: 10  LLLLVIIFFNSALATQLTFSVVNSGAKPDAKIDSSKAFQLAWERACSSSQAATIYVPEGK 69
           LL+L + FF S+ + Q +++V++ GAKPD K D++KAF   W+ AC+SS+  TI VP+G+
Sbjct: 4   LLVLFVFFFISSCSAQ-SYNVLSFGAKPDGKTDATKAFMAVWQTACASSRPVTIVVPKGR 63

Query: 70  FYLHSVTFKG-PCNNNAITIRIDGTLVAPSDFHVTDPQN-WIKFHLVDGVTILGGVLDGQ 129
           F L SVTF G  C    +T RIDGTLVAP+D+ V   ++ WI F  +DG+T+ GGVLD +
Sbjct: 64  FLLRSVTFDGSKCKPKPVTFRIDGTLVAPADYRVIGNEDYWIFFQHLDGITVYGGVLDAR 123

Query: 130 GTGLWDCKHFGKNCPRGATSLKFTNSQNIMISGLTSLDSQLFHIVVNECRNVKLQGLKVS 189
           G  LWDCK  GKNCP GAT++ F +S N+++SGLTSL+SQ+FH+V+N C NVKLQG+KV 
Sbjct: 124 GASLWDCKKSGKNCPSGATTIGFQSSSNVVVSGLTSLNSQMFHVVINGCNNVKLQGVKVL 183

Query: 190 AAGNSPNTDGIHIGLSTYVTILNSNISTGDDCVSIGPGTSNLWIENVACGPGHGISIGSL 249
           AAGNSPNTDGIH+  S+ V+I N+ ISTGDDCVSIGPGT+ LWIENVACGPGHGISIGSL
Sbjct: 184 AAGNSPNTDGIHVQSSSSVSIFNTKISTGDDCVSIGPGTNGLWIENVACGPGHGISIGSL 243

Query: 250 GRNLKEDGVQNVT----------------------------------------------- 306
           G++  E GVQNVT                                               
Sbjct: 244 GKDSVESGVQNVTVKTVTFTGTDNGVRIKSWARPSSGFAKNIRFQHCVMNNVENPIIIDQ 303

[Close]

BLAST of Spg010157 vs. TAIR 10
Match: AT3G59850.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 340.1 bits (871), Expect = 1.8e-93
Identity = 180/390 (46.15%), Postives = 231/390 (59.23%), Query Frame = 0


Query: 3   LHTSLCLLLLLVIIFFNSALATQLTFSVVNSGAKPDAKIDSSKAFQLAWERACSSSQAAT 62
           +H+SL ++LL ++   +S+     T+++++ GAKPD K DS+KAF + W +AC+S +  T
Sbjct: 1   MHSSLLIVLLFLLSVSSSSAQ---TYNILSYGAKPDGKTDSTKAFTVLWAKACASVKPVT 60

Query: 63  IYVPEGKFYLHSVTFKG-PCNNNAITIRIDGTLVAPSDFHVTDPQN-WIKFHLVDGVTIL 122
           I VP+G+F L S+ F G  C   ++T RI GTLVAPSD+ V   +N WI F  +DG+++ 
Sbjct: 61  ILVPKGRFLLRSIIFDGSKCKRKSVTFRIQGTLVAPSDYRVIGKENYWILFQHLDGISVY 120

Query: 123 GGVLDGQGTGLWDCKHFGKNCPRGATSLKFTNSQNIMISGLTSLDSQLFHIVVNECRNVK 182
           GGVLD QG  LW CK  GKNCP GATS+ F +S+N++ISGLTSL+SQ+FH+ +N C NVK
Sbjct: 121 GGVLDAQGASLWSCKKSGKNCPSGATSIGFQSSRNVVISGLTSLNSQMFHVAINGCSNVK 180

Query: 183 LQGLKVSAAGNSPNTDGIHIGLSTYVTILNSNISTGDDCVSIGPGTSNLWIENVACGPGH 242
           L G+KVSA GNSPNTDGIH+  S+ V+ILNS ISTGDDCVSIGPGT+ LWIENVACGPGH
Sbjct: 181 LDGVKVSADGNSPNTDGIHVQSSSTVSILNSKISTGDDCVSIGPGTNGLWIENVACGPGH 240

Query: 243 GISIGSLGRNLKEDGVQNVT---------------------------------------- 302
           GISIGSLG+   E GVQN+T                                        
Sbjct: 241 GISIGSLGKESVEVGVQNITVKTATFTGTENGVRIKSWARPSNGFAKNIRFQHCVMNNVQ 300

Query: 303 ------------------------------------------VKFDCSPKFPCTGIKLED 309
                                                     VK DCS K PCTGI+++D
Sbjct: 301 NPIVIDQNYCPGNENCPNQVSGIKISDVMFFDIHGTSATEVGVKLDCSSKKPCTGIRIQD 360

[Close]

BLAST of Spg010157 vs. TAIR 10
Match: AT1G65570.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 272.7 bits (696), Expect = 3.6e-73
Identity = 148/396 (37.37%), Postives = 208/396 (52.53%), Query Frame = 0


Query: 1   MALHTSLCLLLLLVIIFFNSALA---TQLTFSVVNSGAKPDAKIDSSKAFQLAWERACSS 60
           MAL  S   +  +VI    S       + + +V++ GA P+  ++S+KAF  AW+ AC  
Sbjct: 1   MALFLSFVQVFSIVITIIMSHFGQFDARTSLNVLSFGANPNGIVESAKAFSDAWDAACGV 60

Query: 61  SQAATIYVPEGKFYLH-SVTFKG-PCNNNAITIRIDGTLVAPSDFHVT-DPQNWIKFHLV 120
             +  IYVP+G++ +   V F+G  C +  IT+RIDGTL+ P D+ +    +NW  F  V
Sbjct: 61  EDSVVIYVPKGRYLVSGEVRFEGESCKSREITLRIDGTLIGPQDYSLLGKKENWFSFSGV 120

Query: 121 DGVTILGGVLDGQGTGLWDCKHFGKNCPRGATSLKFTNSQNIMISGLTSLDSQLFHIVVN 180
             VT+LGG  D +G+ LW CK  G NCP GAT+L+F +S N+ I G+ SL+SQLFHI +N
Sbjct: 121 HNVTVLGGSFDAKGSTLWSCKANGYNCPEGATTLRFMDSNNVKIKGVLSLNSQLFHIAIN 180

Query: 181 ECRNVKLQGLKVSAAGNSPNTDGIHIGLSTYVTILNSNISTGDDCVSIGPGTSNLWIENV 240
            CRN+K++ +++ A   SPNTDGIHI LST + + N++I TGDDC+SIGPGT NL ++ +
Sbjct: 181 RCRNIKIEDVRIIAPDESPNTDGIHIQLSTDIEVRNASIKTGDDCISIGPGTKNLMVDGI 240

Query: 241 ACGPGHGISIGSLGRNLKEDGVQNVTV--------------------------------- 300
            CGPGHGISIGSL ++++E GV+NVTV                                 
Sbjct: 241 TCGPGHGISIGSLAKSIEEQGVENVTVKNAVFVRTDNGLRIKSWPRHSNGFVERVRFLGA 300

Query: 301 -------------------------------------------------KFDCSPKFPCT 309
                                                            K DCS K PCT
Sbjct: 301 IMVNVSYPILIDQNYCPGDSSCPSQESGIKINDVIYSGIMGTSATEIAIKMDCSEKVPCT 360

[Close]

BLAST of Spg010157 vs. TAIR 10
Match: AT2G43860.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 267.7 bits (683), Expect = 1.2e-71
Identity = 158/396 (39.90%), Postives = 210/396 (53.03%), Query Frame = 0


Query: 3   LHTSLCLLLLLVIIFFNSALATQL------TFSVVNSGAKPDAKIDSSKAFQLAWERACS 62
           ++ + CL+L L +I  +  L + L      T +V++ GAKPD   DS+KAF  AW+ AC+
Sbjct: 2   VYNTSCLILPLALIILDFTLISSLAHPIPSTLNVLSYGAKPDGSKDSTKAFLAAWDVACA 61

Query: 63  SSQAATIYVPEGKFYLHSVTFKG-PCNNNAITIRIDGTLVAPSDFH-VTDPQNWIKFHLV 122
           S+   TI VP+G+F + ++ F G  C    I+IRI G++VAP DF  +   ++WI F  V
Sbjct: 62  SANPTTIIVPKGRFLVGNLVFHGNECKQAPISIRIAGSIVAPEDFRIIASSKHWIWFEDV 121

Query: 123 DGVTILGGVLDGQGTGLWDCK-HFGKNCPRGATSLKFTNSQNIMISGLTSLDSQLFHIVV 182
             V+I GG+LD QGT LW CK + G NCP GA SL F+ S NI ISGLTS++SQ FHIV+
Sbjct: 122 TDVSIYGGILDAQGTSLWKCKNNGGHNCPTGAKSLVFSGSNNIKISGLTSINSQKFHIVI 181

Query: 183 NECRNVKLQGLKVSAAGNSPNTDGIHIGLSTYVTILNSNISTGDDCVSIGPGTSNLWIEN 242
           +   NV + G+KVSA  NSPNTDGIH+  S  V I NS I TGDDC+SIGPG++N++I+ 
Sbjct: 182 DNSNNVNIDGVKVSADENSPNTDGIHVESSHSVHITNSRIGTGDDCISIGPGSTNVFIQT 241

Query: 243 VACGPGHGISIGSLGRNLKEDGVQNVTVK------------------------------- 302
           + CGPGHGISIGSLGR  +E GV NVTV                                
Sbjct: 242 IRCGPGHGISIGSLGRAEEEQGVDNVTVSNVDFMGTNNGVRIKTWGKDSNSFARNIVFQH 301

Query: 303 --------------------------------------------------FDCSPKFPCT 309
                                                              DCS + PCT
Sbjct: 302 INMKMVKNPIIIDQHYCLHKPCPKQESGVKVSNVRYEDIHGTSNTEVAVLLDCSKEKPCT 361

[Close]

BLAST of Spg010157 vs. TAIR 10
Match: AT1G05660.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 266.2 bits (679), Expect = 3.4e-71
Identity = 143/383 (37.34%), Postives = 203/383 (53.00%), Query Frame = 0


Query: 10  LLLLVIIFFNSALATQLTFSVVNSGAKPDAKIDSSKAFQLAWERACSSSQAATIYVPEGK 69
           LL  ++ F + +++    F+VV+ GAKPD   DS+ AF  AW+ AC S+ +AT+ VP+G 
Sbjct: 10  LLFTLLTFIDVSISASNVFNVVSFGAKPDGVTDSTGAFLKAWQGACVSASSATVVVPKGT 69

Query: 70  FYLHSVTFKGPCNNNAITIRIDGTLVAPSDFHVTDPQN-WIKFHLVDGVTILGGVLDGQG 129
           F L  +TF GPC +  IT ++ GT++AP D+        WI F+ V+  +++GG  D + 
Sbjct: 70  FLLKVITFGGPCKSK-ITFQVAGTVIAPEDYRTFGNSGFWILFNKVNRFSLVGGTFDARA 129

Query: 130 TGLWDCKHFGKNCPRGATSLKFTNSQNIMISGLTSLDSQLFHIVVNECRNVKLQGLKVSA 189
            G W C+  G+NCP G  S+ F ++++++ISG+ S++SQ+ H+ +N C NV ++ +K+ A
Sbjct: 130 NGFWSCRKSGQNCPPGVRSISFNSAKDVIISGVKSMNSQVTHMTLNGCTNVVVRNVKLVA 189

Query: 190 AGNSPNTDGIHIGLSTYVTILNSNISTGDDCVSIGPGTSNLWIENVACGPGHGISIGSLG 249
            GNSPNTDG H+  ST VT   S + TGDDCV+IGPGT NL I  +ACGPGHG+SIGSL 
Sbjct: 190 PGNSPNTDGFHVQHSTGVTFTGSTVQTGDDCVAIGPGTRNLLITKLACGPGHGVSIGSLA 249

Query: 250 RNLKEDGVQNVTV----------------------------------------------- 309
           + LKEDGV+NVTV                                               
Sbjct: 250 KELKEDGVENVTVSSSVFTGSQNGVRIKSWARPSNGFVRTVFFQDLVMKNVENPIIIDQN 309

The following BLAST results are available for this feature:

BLAST of Spg010157 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022953045.1	1.4e-119	60.53	polygalacturonase-like [Cucurbita moschata]	[more]
XP_023511587.1	9.1e-119	60.00	polygalacturonase-like [Cucurbita pepo subsp. pepo]	[more]
XP_022953043.1	1.2e-118	60.26	polygalacturonase-like [Cucurbita moschata]	[more]
XP_023511588.1	3.5e-118	60.00	polygalacturonase-like [Cucurbita pepo subsp. pepo]	[more]
XP_022971874.1	5.0e-117	58.95	polygalacturonase-like [Cucurbita maxima]	[more]

BLAST of Spg010157 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P48979	2.1e-102	51.28	Polygalacturonase OS=Prunus persica OX=3760 PE=2 SV=1	[more]
O22818	1.6e-70	39.90	Probable polygalacturonase At2g43860 OS=Arabidopsis thaliana OX=3702 GN=At2g4386...	[more]
Q6H9K0	2.8e-54	43.08	Exopolygalacturonase (Fragment) OS=Platanus acerifolia OX=140101 GN=plaa2 PE=1 S...	[more]
P35338	1.7e-51	42.75	Exopolygalacturonase OS=Zea mays OX=4577 GN=PG9 PE=2 SV=1	[more]
P49063	2.2e-51	43.82	Exopolygalacturonase clone GBGA483 OS=Arabidopsis thaliana OX=3702 GN=At3g07850 ...	[more]

BLAST of Spg010157 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GNK7	6.8e-120	60.53	polygalacturonase-like OS=Cucurbita moschata OX=3662 GN=LOC111455565 PE=3 SV=1	[more]
A0A6J1GMA1	5.8e-119	60.26	polygalacturonase-like OS=Cucurbita moschata OX=3662 GN=LOC111455564 PE=3 SV=1	[more]
A0A6J1I9T6	2.4e-117	58.95	polygalacturonase-like OS=Cucurbita maxima OX=3661 GN=LOC111470567 PE=3 SV=1	[more]
A0A6J1I881	5.0e-115	57.89	polygalacturonase-like OS=Cucurbita maxima OX=3661 GN=LOC111470566 PE=3 SV=1	[more]
A0A1S3BJI1	6.6e-115	56.53	polygalacturonase-like OS=Cucumis melo OX=3656 GN=LOC103490378 PE=3 SV=1	[more]

BLAST of Spg010157 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G43870.1	5.7e-95	48.02	Pectin lyase-like superfamily protein	[more]
AT3G59850.1	1.8e-93	46.15	Pectin lyase-like superfamily protein	[more]
AT1G65570.1	3.6e-73	37.37	Pectin lyase-like superfamily protein	[more]
AT2G43860.1	1.2e-71	39.90	Pectin lyase-like superfamily protein	[more]
AT1G05660.1	3.4e-71	37.34	Pectin lyase-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000743	Glycoside hydrolase, family 28	PFAM	PF00295	Glyco_hydro_28	coord: 55..262 e-value: 9.1E-54 score: 182.7
IPR000743	Glycoside hydrolase, family 28	PROSITE	PS00502	POLYGALACTURONASE	coord: 233..246
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 261..307 e-value: 8.2E-6 score: 27.0 coord: 18..260 e-value: 1.3E-72 score: 247.1
None	No IPR available	PANTHER	PTHR31375	FAMILY NOT NAMED	coord: 259..308
None	No IPR available	PANTHER	PTHR31375:SF219	PUTATIVE-RELATED	coord: 259..308
None	No IPR available	PANTHER	PTHR31375:SF219	PUTATIVE-RELATED	coord: 7..262
None	No IPR available	PANTHER	PTHR31375	FAMILY NOT NAMED	coord: 7..262
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 11..309

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg010157.1	Spg010157.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
molecular_function	GO:0004650	polygalacturonase activity