Spg010133 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg010133
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	3-oxo-5-alpha-steroid 4-dehydrogenase 1-like
Location	scaffold7: 4841802 .. 4844151 (-)
RNA-Seq Expression	Spg010133
Synteny	Spg010133

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATCAGAGAGCAGATTTCTAGAGAAATATTTACACAAAGAGACTTGATCCATCTGGTTCACGGCGGCGATGGATTACTTATCGGAGTTTGTTTTTGCGCCGCCGTCTTCACTGTTCGTGGCCGTGATGACGGTGGTGAGCTCGATGGTGTTGGTGTTGACGGGGCTTTCGGAAGCGAGAGGGAAGCATATGCAGTATTCGAAATTCTGGAACGCGAATGGTGGTACGAAATCGTCTTCGTCGGAGCAGTTCAAACTTCCGTCCAGGTTAGGGATGCTATTGGCTTATACTCCGGCATTTCTTGCCGGCGTTGTGTCGTTGTGGTTGTTTCCGAATGAGAATCGGAGGATTCTTCTGCTTAAGTCTGCTCTCATCTTGCATTTCTTGAAGAGAGACCTTGAGGTAATGTCTCTGTCTCTGTTTATTTCTCTGTGCCTCTGATTGTGTTCTGAATTTCAATCTGATCCTGGAAATCGATAGTGTAGATTGTGGAAATGAACTGTTTCTTCTTGCATTATTCGTGATTTACTGATTGGCTGTTCATTTTCTTGTGAAGAATTTGTTATTCATGCCCTAGTTTGATCTAATCCTGGAAAAATATATGTCCTAGTATGTTAATATGAGCATCATTTCGAACAACATAAATTGGAGATAATTTTGGATAAATGGATATTAAATAGAAAAATAAGATAATGAGGATCAAAATTATTATTTTTTAGATTAAAATAAATATTTTAACACCAATAAAATAATATATTATTGGTTAAAATACCATCGTAGTATCGTGCATTTCATTCCATTTTAATCTATATATTTTCAAATGTCTAAATTTAGTCTTTATAATAATTTTTAAAACTAGTTTTTTGGAGTTAATTTTTGTCAAAATTGGTTTAATAATAATAATAATAATTTTCATGTAAGAAAATACATGGTGTGGATGTGTTTTCAAAACTTATAGAGAAAAAATTAATAAATTATATATTTTTTTAGAATTTTTAACAATAAACTAGCATTTCAACGTTCGAAAGTATAACTAAAAGCAACAAAACACTATATTTTAGGGGATATTATTTTCCAACGTGTGAAATTGTAAACGGAAGAAGCATATATTCCAAATTTTCCTCCCTTCCCATTCCACTTTACATCTCAAGACAGGGATATTTCTGTCATCTTCTATGAGAAATGTCTCGATTGGATTATTTTTGCTGTTCATTTGATATTCGTTTGTTGTTTTTGTGTTTATATACTATATTTGTTCGTGCTATTATTATTTTTTGAGTTCAACGATATTTGAGATAGAGAGATTGAAGCCTCTAATATTTTAGAAGATGATATATGTCAATTACCGTTAAATTATGCTCCTCTTAGTCATATAAACTTTAAATTAGTCCTTGGCCTCATAAAAAAGGTTTATTAAATTGTCACTACACATAATTCATGGCAGTCCCTAAAAAACTCTCTCTGTGTGTGAAAATTGTACAGGTACTGTTTGTTCATAAATACAGCAGCAAAATGGTAATTGACACAGTAATTACAATCTCCCTGAGCTACTTCTCCTCCACAGCACCCATGATATACACTCAGCACCTTTCAAAAGCGCTTCCTGAGCCATCCATTGATCTGAAAAACACTGGAATTGCTCTGTTTGTGATAGGTATTACTGGGAACTTTTACCACCATTTTCTCTTGTCCAAAACCAGAAAACAGGGAGAAACTGGCTACAAAATCCCCAAAGGAGGCCTCTTCAGCATTGTGATCTGCCCTCACTATCTCTTTGAAATTATTGAGTTCTTTGGATTTGCTTTCATCTCTCAGACAATCTACTCACTCTGCTTTGCTTTTGCCACTACTTTCTATTTGGTTGGGAGGAGCCATGCCACTATGAGATGGTATGCCTCTAAATTTCACCACTTCCCCAAACATCTCAAAGCCATTCTCCCCTTTGTATTTTAGCCTTTTCTTTTAGCTCAATCCTGTCGTCCGCGGTTTTCGAACATCTGACCATTAGGACGGATGTTGATAGAAGTTTTATGTCTTAAATAACCGAGTAGCTTTGCTTAGGTTGGCTCTTTTGCAAGAATGTGTGTCGTTTGTAATTTCTCTTGGTTGTCTCGTGCTTTGACCGATGACATTTGTGTTAATGTTAATATTGAATGTTTCAATACAATAATATGGGATGTGGAGATTTGAACATCTGATCACAAAAATAAGGTGTATAATGCTATAACTATTTTTTTTTTTTTTTGAAAATAGAGAATTTCATTCATAAACAAAGGCTCCCATTCGGGGATACAAACCAACACAACTCTCTACTAGCCAAACCTTCCACCCAATCCGGAAAGTTCCTATTCC

mRNA sequence

ATGGATTACTTATCGGAGTTTGTTTTTGCGCCGCCGTCTTCACTGTTCGTGGCCGTGATGACGGTGGTGAGCTCGATGGTGTTGGTGTTGACGGGGCTTTCGGAAGCGAGAGGGAAGCATATGCAGTATTCGAAATTCTGGAACGCGAATGGTGGTACGAAATCGTCTTCGTCGGAGCAGTTCAAACTTCCGTCCAGGTTAGGGATGCTATTGGCTTATACTCCGGCATTTCTTGCCGGCGTTGTGTCGTTGTGGTTGTTTCCGAATGAGAATCGGAGGATTCTTCTGCTTAAGTCTGCTCTCATCTTGCATTTCTTGAAGAGAGACCTTGAGGTACTGTTTGTTCATAAATACAGCAGCAAAATGGTAATTGACACAGTAATTACAATCTCCCTGAGCTACTTCTCCTCCACAGCACCCATGATATACACTCAGCACCTTTCAAAAGCGCTTCCTGAGCCATCCATTGATCTGAAAAACACTGGAATTGCTCTGTTTGTGATAGGTATTACTGGGAACTTTTACCACCATTTTCTCTTGTCCAAAACCAGAAAACAGGGAGAAACTGGCTACAAAATCCCCAAAGGAGGCCTCTTCAGCATTGTGATCTGCCCTCACTATCTCTTTGAAATTATTGAGTTCTTTGGATTTGCTTTCATCTCTCAGACAATCTACTCACTCTGCTTTGCTTTTGCCACTACTTTCTATTTGGTTGGGAGGAGCCATGCCACTATGAGATGGTATGCCTCTAAATTTCACCACTTCCCCAAACATCTCAAAGCCATTCTCCCCTTTGTATTTTAG

Coding sequence (CDS)

ATGGATTACTTATCGGAGTTTGTTTTTGCGCCGCCGTCTTCACTGTTCGTGGCCGTGATGACGGTGGTGAGCTCGATGGTGTTGGTGTTGACGGGGCTTTCGGAAGCGAGAGGGAAGCATATGCAGTATTCGAAATTCTGGAACGCGAATGGTGGTACGAAATCGTCTTCGTCGGAGCAGTTCAAACTTCCGTCCAGGTTAGGGATGCTATTGGCTTATACTCCGGCATTTCTTGCCGGCGTTGTGTCGTTGTGGTTGTTTCCGAATGAGAATCGGAGGATTCTTCTGCTTAAGTCTGCTCTCATCTTGCATTTCTTGAAGAGAGACCTTGAGGTACTGTTTGTTCATAAATACAGCAGCAAAATGGTAATTGACACAGTAATTACAATCTCCCTGAGCTACTTCTCCTCCACAGCACCCATGATATACACTCAGCACCTTTCAAAAGCGCTTCCTGAGCCATCCATTGATCTGAAAAACACTGGAATTGCTCTGTTTGTGATAGGTATTACTGGGAACTTTTACCACCATTTTCTCTTGTCCAAAACCAGAAAACAGGGAGAAACTGGCTACAAAATCCCCAAAGGAGGCCTCTTCAGCATTGTGATCTGCCCTCACTATCTCTTTGAAATTATTGAGTTCTTTGGATTTGCTTTCATCTCTCAGACAATCTACTCACTCTGCTTTGCTTTTGCCACTACTTTCTATTTGGTTGGGAGGAGCCATGCCACTATGAGATGGTATGCCTCTAAATTTCACCACTTCCCCAAACATCTCAAAGCCATTCTCCCCTTTGTATTTTAG

Protein sequence

MDYLSEFVFAPPSSLFVAVMTVVSSMVLVLTGLSEARGKHMQYSKFWNANGGTKSSSSEQFKLPSRLGMLLAYTPAFLAGVVSLWLFPNENRRILLLKSALILHFLKRDLEVLFVHKYSSKMVIDTVITISLSYFSSTAPMIYTQHLSKALPEPSIDLKNTGIALFVIGITGNFYHHFLLSKTRKQGETGYKIPKGGLFSIVICPHYLFEIIEFFGFAFISQTIYSLCFAFATTFYLVGRSHATMRWYASKFHHFPKHLKAILPFVF

Homology

[Close]

BLAST of Spg010133 vs. NCBI nr
Match: KAG6573901.1 (3-oxo-5-alpha-steroid 4-dehydrogenase 1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7012967.1 3-oxo-5-alpha-steroid 4-dehydrogenase 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 420.6 bits (1080), Expect = 9.9e-114
Identity = 217/267 (81.27%), Postives = 232/267 (86.89%), Query Frame = 0


Query: 1   MDYLSEFVFAPPSSLFVAVMTVVSSMVLVLTGLSEARGKHMQYSKFWNANGGTKSSSSEQ 60
           MD LSEFVFAPP SLF+ V+TVV  +V+ + GLSEARG+H+QYSKFWNAN   K  S EQ
Sbjct: 1   MDALSEFVFAPPPSLFLNVVTVVIPVVVAMLGLSEARGRHLQYSKFWNANSAPK--SLEQ 60

Query: 61  FKLPSRLGMLLAYTPAFLAGVVSLWLFPNENRRILLLKSALILHFLKRDLEVLFVHKYSS 120
           FKLPS LGMLLAYTPA LAGVVSLW FPNE+RR LLLKSAL LHFLKRDLEVLFVHKYSS
Sbjct: 61  FKLPSWLGMLLAYTPALLAGVVSLWFFPNEDRRTLLLKSALTLHFLKRDLEVLFVHKYSS 120

Query: 121 KMVIDTVITISLSYFSSTAPMIYTQHLSKALPEPSIDLKNTGIALFVIGITGNFYHHFLL 180
           KMV DT ITIS SYFSSTA MIYTQHLSKALPEPSIDLKN GIALF+IGI GNFYHHFLL
Sbjct: 121 KMVFDTAITISSSYFSSTALMIYTQHLSKALPEPSIDLKNIGIALFLIGIIGNFYHHFLL 180

Query: 181 SKTRKQGETGYKIPKGGLFSIVICPHYLFEIIEFFGFAFISQTIYSLCFAFATTFYLVGR 240
           SKTRKQGETGYKIP+GGLF+ VICPHYLFEIIE+FGFAFISQT +SL FAF T FYL GR
Sbjct: 181 SKTRKQGETGYKIPQGGLFNTVICPHYLFEIIEYFGFAFISQTFFSLSFAFGTAFYLAGR 240

Query: 241 SHATMRWYASKFHHFPKHLKAILPFVF 268
           S AT RWYASKF  FP H+KA+LPFVF
Sbjct: 241 SSATKRWYASKFEDFPNHIKALLPFVF 265

[Close]

BLAST of Spg010133 vs. NCBI nr
Match: XP_022945516.1 (3-oxo-5-alpha-steroid 4-dehydrogenase 1-like [Cucurbita moschata])

HSP 1 Score: 420.6 bits (1080), Expect = 9.9e-114
Identity = 217/267 (81.27%), Postives = 232/267 (86.89%), Query Frame = 0


Query: 1   MDYLSEFVFAPPSSLFVAVMTVVSSMVLVLTGLSEARGKHMQYSKFWNANGGTKSSSSEQ 60
           MD LSEFVFAPP SLF+ V+TVV  +V+ + GLSEARG+H+QYSKFWNAN   K  S EQ
Sbjct: 1   MDALSEFVFAPPPSLFLNVVTVVIPVVVAMLGLSEARGRHLQYSKFWNANSSPK--SLEQ 60

Query: 61  FKLPSRLGMLLAYTPAFLAGVVSLWLFPNENRRILLLKSALILHFLKRDLEVLFVHKYSS 120
           FKLPS LGMLLAYTPA LAGVVSLW FPNE+RR LLLKSAL LHFLKRDLEVLFVHKYSS
Sbjct: 61  FKLPSWLGMLLAYTPALLAGVVSLWFFPNEDRRTLLLKSALTLHFLKRDLEVLFVHKYSS 120

Query: 121 KMVIDTVITISLSYFSSTAPMIYTQHLSKALPEPSIDLKNTGIALFVIGITGNFYHHFLL 180
           KMV DT ITIS SYFSSTA MIYTQHLSKALPEPSIDLKN GIALF+IGI GNFYHHFLL
Sbjct: 121 KMVFDTAITISSSYFSSTALMIYTQHLSKALPEPSIDLKNIGIALFLIGIIGNFYHHFLL 180

Query: 181 SKTRKQGETGYKIPKGGLFSIVICPHYLFEIIEFFGFAFISQTIYSLCFAFATTFYLVGR 240
           SKTRKQGETGYKIP+GGLF+ VICPHYLFEIIE+FGFAFISQT +SL FAF T FYL GR
Sbjct: 181 SKTRKQGETGYKIPQGGLFNTVICPHYLFEIIEYFGFAFISQTFFSLSFAFGTAFYLAGR 240

Query: 241 SHATMRWYASKFHHFPKHLKAILPFVF 268
           S AT RWYASKF  FP H+KA+LPFVF
Sbjct: 241 SSATKRWYASKFEDFPNHIKALLPFVF 265

[Close]

BLAST of Spg010133 vs. NCBI nr
Match: XP_023542215.1 (3-oxo-5-alpha-steroid 4-dehydrogenase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 418.3 bits (1074), Expect = 4.9e-113
Identity = 216/267 (80.90%), Postives = 231/267 (86.52%), Query Frame = 0


Query: 1   MDYLSEFVFAPPSSLFVAVMTVVSSMVLVLTGLSEARGKHMQYSKFWNANGGTKSSSSEQ 60
           MD LSEFVFAPP SLF+ V+TVV  +V+ + GLSEARG+H+QYSKFWNAN   K  S EQ
Sbjct: 1   MDALSEFVFAPPPSLFLNVVTVVIPVVVAMLGLSEARGRHLQYSKFWNANSAPK--SLEQ 60

Query: 61  FKLPSRLGMLLAYTPAFLAGVVSLWLFPNENRRILLLKSALILHFLKRDLEVLFVHKYSS 120
           FKLPS LGMLLAYTPA LAGVVSLW FPNE+RR LLLKSAL LHFLKRDLEVLFVHKYSS
Sbjct: 61  FKLPSWLGMLLAYTPALLAGVVSLWFFPNEDRRTLLLKSALTLHFLKRDLEVLFVHKYSS 120

Query: 121 KMVIDTVITISLSYFSSTAPMIYTQHLSKALPEPSIDLKNTGIALFVIGITGNFYHHFLL 180
           KMV DT ITIS SYFSSTA MIYTQHLSKALPEPSIDLKN GIALF+IGI GNFYHHFLL
Sbjct: 121 KMVFDTAITISSSYFSSTALMIYTQHLSKALPEPSIDLKNIGIALFLIGIIGNFYHHFLL 180

Query: 181 SKTRKQGETGYKIPKGGLFSIVICPHYLFEIIEFFGFAFISQTIYSLCFAFATTFYLVGR 240
           SKTRKQGETGYKIP+GGLF+ VICPHYLFEIIE+FGFA ISQT +SL FAF T FYL GR
Sbjct: 181 SKTRKQGETGYKIPQGGLFNTVICPHYLFEIIEYFGFALISQTFFSLSFAFGTAFYLAGR 240

Query: 241 SHATMRWYASKFHHFPKHLKAILPFVF 268
           S AT RWYASKF  FP H+KA+LPFVF
Sbjct: 241 SSATKRWYASKFEDFPNHIKALLPFVF 265

[Close]

BLAST of Spg010133 vs. NCBI nr
Match: XP_022968321.1 (3-oxo-5-alpha-steroid 4-dehydrogenase 1-like [Cucurbita maxima])

HSP 1 Score: 413.7 bits (1062), Expect = 1.2e-111
Identity = 212/267 (79.40%), Postives = 231/267 (86.52%), Query Frame = 0


Query: 1   MDYLSEFVFAPPSSLFVAVMTVVSSMVLVLTGLSEARGKHMQYSKFWNANGGTKSSSSEQ 60
           MD LSEFVFAPP SLF+ V+TVV  +V+ + GLSEARG+H+QYSKFWNAN   K  S E+
Sbjct: 1   MDALSEFVFAPPPSLFLNVVTVVIPVVVAMLGLSEARGRHLQYSKFWNANSAPK--SLEK 60

Query: 61  FKLPSRLGMLLAYTPAFLAGVVSLWLFPNENRRILLLKSALILHFLKRDLEVLFVHKYSS 120
           FKLPS LGMLLAYTPA LAGV+SLW FPNE+RR LLLKSAL LHFLKRDLEVLFVHKYSS
Sbjct: 61  FKLPSWLGMLLAYTPALLAGVLSLWFFPNEDRRTLLLKSALTLHFLKRDLEVLFVHKYSS 120

Query: 121 KMVIDTVITISLSYFSSTAPMIYTQHLSKALPEPSIDLKNTGIALFVIGITGNFYHHFLL 180
           KMV DT I IS SYFSSTA MIYTQHLSKALPEPSIDLKN G+ALF+IGI GNFYHHFLL
Sbjct: 121 KMVFDTAIIISSSYFSSTALMIYTQHLSKALPEPSIDLKNIGLALFLIGIIGNFYHHFLL 180

Query: 181 SKTRKQGETGYKIPKGGLFSIVICPHYLFEIIEFFGFAFISQTIYSLCFAFATTFYLVGR 240
           SKTRKQGETGY+IP+GGLF+ VICPHYLFEIIE+FGFAFISQT +SL FAF T FYL GR
Sbjct: 181 SKTRKQGETGYRIPQGGLFNTVICPHYLFEIIEYFGFAFISQTFFSLSFAFGTAFYLAGR 240

Query: 241 SHATMRWYASKFHHFPKHLKAILPFVF 268
           S AT RWYASKF  FP H+KA+LPFVF
Sbjct: 241 SSATKRWYASKFEDFPNHIKALLPFVF 265

[Close]

BLAST of Spg010133 vs. NCBI nr
Match: XP_038892898.1 (3-oxo-5-alpha-steroid 4-dehydrogenase 2-like [Benincasa hispida])

HSP 1 Score: 400.6 bits (1028), Expect = 1.1e-107
Identity = 207/267 (77.53%), Postives = 227/267 (85.02%), Query Frame = 0


Query: 1   MDYLSEFVFAPPSSLFVAVMTVVSSMVLVLTGLSEARGKHMQYSKFWNANGGTKSSSSEQ 60
           MD +SEF F PPSSLFV VMTVVSS V+V+ GLSE RGK+M+YSKFWN       SS+  
Sbjct: 1   MDTVSEFFFPPPSSLFVNVMTVVSSAVVVVVGLSETRGKNMKYSKFWNNTNNAPKSSN-- 60

Query: 61  FKLPSRLGMLLAYTPAFLAGVVSLWLFPNENRRILLLKSALILHFLKRDLEVLFVHKYSS 120
             L +RLGMLLAY PAFL GVV LWLFPNE+RRILLLKSALILHF KR+LEVLF+HKYSS
Sbjct: 61  --LSARLGMLLAYMPAFLVGVVLLWLFPNEDRRILLLKSALILHFFKRNLEVLFLHKYSS 120

Query: 121 KMVIDTVITISLSYFSSTAPMIYTQHLSKALPEPSIDLKNTGIALFVIGITGNFYHHFLL 180
           KMVIDTVITISLSYFSSTA MIYTQHLS+ LPEPSIDLK  GIAL++IGITGNFYHH+LL
Sbjct: 121 KMVIDTVITISLSYFSSTAIMIYTQHLSQGLPEPSIDLKKIGIALYLIGITGNFYHHYLL 180

Query: 181 SKTRKQGETGYKIPKGGLFSIVICPHYLFEIIEFFGFAFISQTIYSLCFAFATTFYLVGR 240
           SK RKQGET YKIPKGGLFS +ICPHYLFEIIEFFGFAFISQTIYSL FA AT  YL GR
Sbjct: 181 SKARKQGETSYKIPKGGLFSFIICPHYLFEIIEFFGFAFISQTIYSLFFAIATALYLTGR 240

Query: 241 SHATMRWYASKFHHFPKHLKAILPFVF 268
           S+AT +WY SKF  FP H+KA+LPF+F
Sbjct: 241 SYATRKWYVSKFEDFPSHVKALLPFLF 263

[Close]

BLAST of Spg010133 vs. ExPASy Swiss-Prot
Match: P18405 (3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Homo sapiens OX=9606 GN=SRD5A1 PE=1 SV=1)

HSP 1 Score: 88.6 bits (218), Expect = 1.2e-16
Identity = 44/106 (41.51%), Postives = 62/106 (58.49%), Query Frame = 0


Query: 162 GIALFVIGITGNFYHHFLLSKTRKQGETGYKIPKGGLFSIVICPHYLFEIIEFFGFAFIS 221
           G  L++ G+  N +   +L   RK G+TGYKIP+GGLF  V   +Y  EI+E+ G+A  S
Sbjct: 154 GFGLWLTGMLINIHSDHILRNLRKPGDTGYKIPRGGLFEYVTAANYFGEIMEWCGYALAS 213

Query: 222 QTIYSLCFAFATTFYLVGRSHATMRWYASKFHHFPKHLKAILPFVF 268
            ++    FAF T  +L GR+     WY  KF  +PK  K I+PF+F
Sbjct: 214 WSVQGAAFAFFTFCFLSGRAKEHHEWYLRKFEEYPKFRKIIIPFLF 259

[Close]

BLAST of Spg010133 vs. ExPASy Swiss-Prot
Match: P24008 (3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Rattus norvegicus OX=10116 GN=Srd5a1 PE=1 SV=2)

HSP 1 Score: 88.6 bits (218), Expect = 1.2e-16
Identity = 44/106 (41.51%), Postives = 64/106 (60.38%), Query Frame = 0


Query: 161 TGIALFVIGITGNFYHHFLLSKTRKQGETGYKIPKGGLFSIVICPHYLFEIIEFFGFAFI 220
           TG AL+++G+  N +   +L   RK GETGYKIP+GGLF  V   +Y  E++E+ GFA  
Sbjct: 153 TGFALWLVGMVINIHSDHILRNLRKPGETGYKIPRGGLFEYVSAANYFGELVEWCGFALA 212

Query: 221 SQTIYSLCFAFATTFYLVGRSHATMRWYASKFHHFPKHLKAILPFV 267
           S ++  + FA  T   L+ R+    +WY  KF  +PK  K ++PFV
Sbjct: 213 SWSLQGVVFALFTLSTLLTRAKQHHQWYHEKFEDYPKSRKILIPFV 258

[Close]

BLAST of Spg010133 vs. ExPASy Swiss-Prot
Match: Q28891 (3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Macaca fascicularis OX=9541 GN=SRD5A1 PE=1 SV=1)

HSP 1 Score: 88.2 bits (217), Expect = 1.5e-16
Identity = 64/224 (28.57%), Postives = 110/224 (49.11%), Query Frame = 0


Query: 52  GTKSSSSEQFKLPSRLGMLLAYTPAFLAGVVSLWLFPNENRRIL------LLKSALILHF 111
           G  +S S + ++P+R   ++   P+     + L+ + +E+   L      +L +  ++H+
Sbjct: 43  GRHASPSFRVRVPARAAWVVQELPSL---ALPLYQYASESAPRLRSAPNCILLAMFLVHY 102

Query: 112 LKRDLEVLFVHKYSSKM-VIDTVITISLSYFSSTAPMIYTQHLSKALPEPSIDLK-NTGI 171
             R L   F+ +    M ++   + I    F+      Y  H +    +   D +   G 
Sbjct: 103 GHRCLIYPFLMRGGKPMPLLACTMAIMFCTFNGYLQSRYLSHWAVYADDWVTDPRFLIGF 162

Query: 172 ALFVIGITGNFYHHFLLSKTRKQGETGYKIPKGGLFSIVICPHYLFEIIEFFGFAFISQT 231
            L++ G+  N +   +L   RK G+TGYKIP+GGLF  V   +Y  EI+E+ G+A  S +
Sbjct: 163 GLWLAGMLINIHSDHILRNLRKPGDTGYKIPRGGLFEYVTAANYFGEIMEWCGYALASWS 222

Query: 232 IYSLCFAFATTFYLVGRSHATMRWYASKFHHFPKHLKAILPFVF 268
           +    FAF T  +L GR+    RWY  KF  +PK  K ++PF+F
Sbjct: 223 VQGAAFAFFTFCFLSGRAKEHHRWYLQKFEEYPKFRKILIPFLF 263

[Close]

BLAST of Spg010133 vs. ExPASy Swiss-Prot
Match: Q68FF9 (3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Mus musculus OX=10090 GN=Srd5a1 PE=2 SV=2)

HSP 1 Score: 86.7 bits (213), Expect = 4.5e-16
Identity = 43/106 (40.57%), Postives = 63/106 (59.43%), Query Frame = 0


Query: 161 TGIALFVIGITGNFYHHFLLSKTRKQGETGYKIPKGGLFSIVICPHYLFEIIEFFGFAFI 220
           TG AL+++G+  N +   +L   RK GETGYKIP+GGLF  V   +Y  E++E+ GFA  
Sbjct: 149 TGFALWLVGMVINIHSDHILRNLRKPGETGYKIPRGGLFEYVSSANYFGELVEWCGFALA 208

Query: 221 SQTIYSLCFAFATTFYLVGRSHATMRWYASKFHHFPKHLKAILPFV 267
           S ++  + FA  T   L  R+    +WY  KF  +PK  K ++PF+
Sbjct: 209 SWSLQGVVFALFTLCALFTRARQHHQWYLEKFEDYPKTRKILIPFL 254

[Close]

BLAST of Spg010133 vs. ExPASy Swiss-Prot
Match: Q28892 (3-oxo-5-alpha-steroid 4-dehydrogenase 2 OS=Macaca fascicularis OX=9541 GN=SRD5A2 PE=1 SV=1)

HSP 1 Score: 83.6 bits (205), Expect = 3.8e-15
Identity = 42/106 (39.62%), Postives = 68/106 (64.15%), Query Frame = 0


Query: 162 GIALFVIGITGNFYHHFLLSKTRKQGETGYKIPKGGLFSIVICPHYLFEIIEFFGFAFIS 221
           G+ LF++G+  N +  ++L + RK GE  Y+IPKGGLF+ V   ++L EIIE+ G+A  +
Sbjct: 149 GVFLFILGMGVNIHSDYILRQLRKPGEITYRIPKGGLFTYVSGANFLGEIIEWIGYALAT 208

Query: 222 QTIYSLCFAFATTFYLVGRSHATMRWYASKFHHFPKHLKAILPFVF 268
            ++ +L FAF +  +L  R+    R+Y   F  +PK  KA++PF+F
Sbjct: 209 WSLPALAFAFFSVCFLGLRAFHHHRFYLKMFEDYPKSRKALIPFIF 254

[Close]

BLAST of Spg010133 vs. ExPASy TrEMBL
Match: A0A6J1G134 (3-oxo-5-alpha-steroid 4-dehydrogenase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111449728 PE=3 SV=1)

HSP 1 Score: 420.6 bits (1080), Expect = 4.8e-114
Identity = 217/267 (81.27%), Postives = 232/267 (86.89%), Query Frame = 0


Query: 1   MDYLSEFVFAPPSSLFVAVMTVVSSMVLVLTGLSEARGKHMQYSKFWNANGGTKSSSSEQ 60
           MD LSEFVFAPP SLF+ V+TVV  +V+ + GLSEARG+H+QYSKFWNAN   K  S EQ
Sbjct: 1   MDALSEFVFAPPPSLFLNVVTVVIPVVVAMLGLSEARGRHLQYSKFWNANSSPK--SLEQ 60

Query: 61  FKLPSRLGMLLAYTPAFLAGVVSLWLFPNENRRILLLKSALILHFLKRDLEVLFVHKYSS 120
           FKLPS LGMLLAYTPA LAGVVSLW FPNE+RR LLLKSAL LHFLKRDLEVLFVHKYSS
Sbjct: 61  FKLPSWLGMLLAYTPALLAGVVSLWFFPNEDRRTLLLKSALTLHFLKRDLEVLFVHKYSS 120

Query: 121 KMVIDTVITISLSYFSSTAPMIYTQHLSKALPEPSIDLKNTGIALFVIGITGNFYHHFLL 180
           KMV DT ITIS SYFSSTA MIYTQHLSKALPEPSIDLKN GIALF+IGI GNFYHHFLL
Sbjct: 121 KMVFDTAITISSSYFSSTALMIYTQHLSKALPEPSIDLKNIGIALFLIGIIGNFYHHFLL 180

Query: 181 SKTRKQGETGYKIPKGGLFSIVICPHYLFEIIEFFGFAFISQTIYSLCFAFATTFYLVGR 240
           SKTRKQGETGYKIP+GGLF+ VICPHYLFEIIE+FGFAFISQT +SL FAF T FYL GR
Sbjct: 181 SKTRKQGETGYKIPQGGLFNTVICPHYLFEIIEYFGFAFISQTFFSLSFAFGTAFYLAGR 240

Query: 241 SHATMRWYASKFHHFPKHLKAILPFVF 268
           S AT RWYASKF  FP H+KA+LPFVF
Sbjct: 241 SSATKRWYASKFEDFPNHIKALLPFVF 265

[Close]

BLAST of Spg010133 vs. ExPASy TrEMBL
Match: A0A6J1HUJ4 (3-oxo-5-alpha-steroid 4-dehydrogenase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111467586 PE=3 SV=1)

HSP 1 Score: 413.7 bits (1062), Expect = 5.9e-112
Identity = 212/267 (79.40%), Postives = 231/267 (86.52%), Query Frame = 0


Query: 1   MDYLSEFVFAPPSSLFVAVMTVVSSMVLVLTGLSEARGKHMQYSKFWNANGGTKSSSSEQ 60
           MD LSEFVFAPP SLF+ V+TVV  +V+ + GLSEARG+H+QYSKFWNAN   K  S E+
Sbjct: 1   MDALSEFVFAPPPSLFLNVVTVVIPVVVAMLGLSEARGRHLQYSKFWNANSAPK--SLEK 60

Query: 61  FKLPSRLGMLLAYTPAFLAGVVSLWLFPNENRRILLLKSALILHFLKRDLEVLFVHKYSS 120
           FKLPS LGMLLAYTPA LAGV+SLW FPNE+RR LLLKSAL LHFLKRDLEVLFVHKYSS
Sbjct: 61  FKLPSWLGMLLAYTPALLAGVLSLWFFPNEDRRTLLLKSALTLHFLKRDLEVLFVHKYSS 120

Query: 121 KMVIDTVITISLSYFSSTAPMIYTQHLSKALPEPSIDLKNTGIALFVIGITGNFYHHFLL 180
           KMV DT I IS SYFSSTA MIYTQHLSKALPEPSIDLKN G+ALF+IGI GNFYHHFLL
Sbjct: 121 KMVFDTAIIISSSYFSSTALMIYTQHLSKALPEPSIDLKNIGLALFLIGIIGNFYHHFLL 180

Query: 181 SKTRKQGETGYKIPKGGLFSIVICPHYLFEIIEFFGFAFISQTIYSLCFAFATTFYLVGR 240
           SKTRKQGETGY+IP+GGLF+ VICPHYLFEIIE+FGFAFISQT +SL FAF T FYL GR
Sbjct: 181 SKTRKQGETGYRIPQGGLFNTVICPHYLFEIIEYFGFAFISQTFFSLSFAFGTAFYLAGR 240

Query: 241 SHATMRWYASKFHHFPKHLKAILPFVF 268
           S AT RWYASKF  FP H+KA+LPFVF
Sbjct: 241 SSATKRWYASKFEDFPNHIKALLPFVF 265

[Close]

BLAST of Spg010133 vs. ExPASy TrEMBL
Match: A0A6J1D974 (3-oxo-5-alpha-steroid 4-dehydrogenase 1-like OS=Momordica charantia OX=3673 GN=LOC111018758 PE=3 SV=1)

HSP 1 Score: 392.9 bits (1008), Expect = 1.1e-105
Identity = 198/267 (74.16%), Postives = 225/267 (84.27%), Query Frame = 0


Query: 1   MDYLSEFVFAPPSSLFVAVMTVVSSMVLVLTGLSEARGKHMQYSKFWNANGGTKSSSSEQ 60
           M+ LSEF+F  P SLFV VMTVVSS V+   G+SEA+GKH+QYSKFWN N   K SSSEQ
Sbjct: 3   MNALSEFLFPAPPSLFVNVMTVVSSAVVTAIGISEAKGKHLQYSKFWNTNTAPK-SSSEQ 62

Query: 61  FKLPSRLGMLLAYTPAFLAGVVSLWLFPNENRRILLLKSALILHFLKRDLEVLFVHKYSS 120
           FK+ SRLGML  YTPAF+AG VSLW FPNE+RRILL+KSAL LHF KRDLEVLFVHKYSS
Sbjct: 63  FKISSRLGMLFLYTPAFIAGAVSLWFFPNEDRRILLIKSALTLHFFKRDLEVLFVHKYSS 122

Query: 121 KMVIDTVITISLSYFSSTAPMIYTQHLSKALPEPSIDLKNTGIALFVIGITGNFYHHFLL 180
           KMV+D+ I ISLSYFSSTA MIY+QHLS+ LP+P IDLKN GIALF+ GI GN YHH +L
Sbjct: 123 KMVLDSAILISLSYFSSTAIMIYSQHLSQGLPQPPIDLKNIGIALFLTGIIGNLYHHLIL 182

Query: 181 SKTRKQGETGYKIPKGGLFSIVICPHYLFEIIEFFGFAFISQTIYSLCFAFATTFYLVGR 240
           + TRK GETGYKIPKGGLF I+ICPHYLFEII +FGFAFISQTIY+LCFA AT  YL GR
Sbjct: 183 ANTRKGGETGYKIPKGGLFGIIICPHYLFEIIVYFGFAFISQTIYALCFALATAVYLGGR 242

Query: 241 SHATMRWYASKFHHFPKHLKAILPFVF 268
           S+AT RWYASKF +FP H+KA++PF+F
Sbjct: 243 SYATRRWYASKFDNFPNHVKALIPFLF 268

[Close]

BLAST of Spg010133 vs. ExPASy TrEMBL
Match: A0A0A0KWK8 (S5A_REDUCTASE domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G612900 PE=3 SV=1)

HSP 1 Score: 388.7 bits (997), Expect = 2.0e-104
Identity = 197/268 (73.51%), Postives = 230/268 (85.82%), Query Frame = 0


Query: 1   MDYLSEFVFAPPSSLFVAVMTVVSSMVLVLTGLSEARGKHMQYSKFWNANGGTKSSSSEQ 60
           MD LSEFVF PP+S FV VMTVVSS ++ + GLSEARGKH++YSKFW++N    ++  + 
Sbjct: 1   MDTLSEFVFPPPASAFVNVMTVVSSAIVAVVGLSEARGKHLKYSKFWSSNNA--NTDRKS 60

Query: 61  FKLPSRLGMLLAYTPAFLAGVVSLWLFPNENRRILLLKSALILHFLKRDLEVLFVHKYSS 120
           F L ++LGML+AY PAFLAGVVSLWLFP+E+RRILLLKSAL+LHF KR+LEVLF+HKYSS
Sbjct: 61  FNLSAKLGMLVAYIPAFLAGVVSLWLFPSEDRRILLLKSALVLHFFKRNLEVLFLHKYSS 120

Query: 121 KMVIDTVITISLSYFSSTAPMIYTQHLSKA-LPEPSIDLKNTGIALFVIGITGNFYHHFL 180
           KM +D+VITIS SYFSSTA MIYTQHLS+  L EP IDLK+ G+ LF+IGI GNFYHH+L
Sbjct: 121 KMAVDSVITISFSYFSSTAIMIYTQHLSQGLLREPPIDLKSIGVGLFLIGIIGNFYHHYL 180

Query: 181 LSKTRKQGETGYKIPKGGLFSIVICPHYLFEIIEFFGFAFISQTIYSLCFAFATTFYLVG 240
           LS+TRKQGET YKIPKGGLFS++ICPHYLFEIIEFFGFAFISQTIYSL FAFAT  YL G
Sbjct: 181 LSQTRKQGETSYKIPKGGLFSLIICPHYLFEIIEFFGFAFISQTIYSLFFAFATALYLSG 240

Query: 241 RSHATMRWYASKFHHFPKHLKAILPFVF 268
           RS+AT +WY SKF  FP H+KA+LPFVF
Sbjct: 241 RSYATRKWYVSKFEDFPIHVKALLPFVF 266

[Close]

BLAST of Spg010133 vs. ExPASy TrEMBL
Match: A0A5D3CDA5 (3-oxo-5-alpha-steroid 4-dehydrogenase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G001760 PE=3 SV=1)

HSP 1 Score: 384.4 bits (986), Expect = 3.8e-103
Identity = 197/268 (73.51%), Postives = 223/268 (83.21%), Query Frame = 0


Query: 1   MDYLSEFVFAPPSSLFVAVMTVVSSMVLVLTGLSEARGKHMQYSKFWNANGGTKSSSSEQ 60
           MD LSEFVF PPSS FV VMTVVSS ++ + GLSEARGKH+QYSKFWN N    ++  + 
Sbjct: 1   MDALSEFVFPPPSSAFVNVMTVVSSAIVAVVGLSEARGKHLQYSKFWN-NNNNANTDRKS 60

Query: 61  FKLPSRLGMLLAYTPAFLAGVVSLWLFPNENRRILLLKSALILHFLKRDLEVLFVHKYSS 120
           F L ++LGMLLAY PAFL GVVS WLFPNE+RRI++LKSAL+LHF KR+LEVLF+HKYSS
Sbjct: 61  FNLSAKLGMLLAYMPAFLVGVVSFWLFPNEDRRIVVLKSALVLHFFKRNLEVLFLHKYSS 120

Query: 121 KMVIDTVITISLSYFSSTAPMIYTQHLSKA-LPEPSIDLKNTGIALFVIGITGNFYHHFL 180
           KM I +VITIS SYFSSTA MIYTQHLS+  LPEP IDLK  G+ LF+IGI GNFYHH+L
Sbjct: 121 KMAIGSVITISFSYFSSTAIMIYTQHLSQGLLPEPPIDLKIIGVGLFLIGIVGNFYHHYL 180

Query: 181 LSKTRKQGETGYKIPKGGLFSIVICPHYLFEIIEFFGFAFISQTIYSLCFAFATTFYLVG 240
           LS TRK GET YKIP+GGLF+ VICPHYLFEIIEFFGFAFISQTIYSL FAFAT  YL G
Sbjct: 181 LSGTRKGGETSYKIPEGGLFNFVICPHYLFEIIEFFGFAFISQTIYSLFFAFATALYLTG 240

Query: 241 RSHATMRWYASKFHHFPKHLKAILPFVF 268
           RS+AT +WY SKF  FP H+KA+LPFVF
Sbjct: 241 RSYATRKWYVSKFEDFPIHVKALLPFVF 267

[Close]

BLAST of Spg010133 vs. TAIR 10
Match: AT5G16010.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 287.7 bits (735), Expect = 9.4e-78
Identity = 148/268 (55.22%), Postives = 188/268 (70.15%), Query Frame = 0


Query: 1   MDYLSEFVFAPPSSLFVAVMTVVSSMVLVLTGLSEARGKHMQYSKFWNANGGTKSSSSEQ 60
           M+ ++ FV+ PP S+ +  M+VV    L   G SE RG H++YSKF  ++   +      
Sbjct: 1   MEMVTSFVYPPPPSILLNCMSVVGVAALANIGWSEIRGNHLKYSKFGVSSSSPQPQKERF 60

Query: 61  FKLPSRLGMLLAYTPAFLAGVVSLWLFPNENRRILLLKSALILHFLKRDLEVLFVHKYSS 120
             + SR GMLL YTPAFLA   S ++ P+++ R LLLKSAL LHF KR  EVLF+HKYS 
Sbjct: 61  GSISSRNGMLLLYTPAFLAAASSFFVVPSDDLRFLLLKSALALHFFKRVFEVLFIHKYSG 120

Query: 121 KMVIDTVITISLSYFSSTAPMIYTQHLSKALPEPSIDLKNTGIALFVIGITGNFYHHFLL 180
            M ID+ +TIS SYFSSTA M+Y+Q+L+  L EPS D+K  G+ +FV+GI GN YHH LL
Sbjct: 121 GMAIDSALTISSSYFSSTALMLYSQNLTLGLTEPSFDMKLAGVVMFVVGIVGNLYHHVLL 180

Query: 181 SKTRKQ-GETGYKIPKGGLFSIVICPHYLFEIIEFFGFAFISQTIYSLCFAFATTFYLVG 240
           +K RK+ G+  YKIPKGGLF I+ICPHYLFEI+ F+ F  ISQTIYS  FA  T  YL+G
Sbjct: 181 AKLRKEDGKKEYKIPKGGLFDIIICPHYLFEILVFWSFFLISQTIYSFSFAMGTMLYLIG 240

Query: 241 RSHATMRWYASKFHHFPKHLKAILPFVF 268
           RS+AT  WY SKF  FPKH+KA++PFVF
Sbjct: 241 RSYATRTWYLSKFDDFPKHIKALIPFVF 268

[Close]

BLAST of Spg010133 vs. TAIR 10
Match: AT2G38050.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 72.0 bits (175), Expect = 8.1e-13
Identity = 38/107 (35.51%), Postives = 62/107 (57.94%), Query Frame = 0


Query: 162 GIALFVIGITGNFYHHFLLSKTRKQGETGYKIPKGGLFSIVICPHYLFEIIEFFGFAFIS 221
           G+ +F+ G+  N      L + +K+   GY IP+GG F +V CP+Y  E IE+ G+A ++
Sbjct: 156 GMVVFITGMYINITSDRTLVRLKKENRGGYVIPRGGWFELVSCPNYFGEAIEWLGWAVMT 215

Query: 222 QTIYSLCFAFATTFYLVGRSHATMRWYASKF-HHFPKHLKAILPFVF 268
            +   + F   T   L  R+ A+ +WY +KF   +PK  KA++PFV+
Sbjct: 216 WSWAGIGFFLYTCSNLFPRARASHKWYIAKFKEEYPKTRKAVIPFVY 262

[Close]

BLAST of Spg010133 vs. TAIR 10
Match: AT3G55360.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 65.1 bits (157), Expect = 9.9e-11
Identity = 52/150 (34.67%), Postives = 76/150 (50.67%), Query Frame = 0


Query: 104 HFLKRDLEVLFVHKYS-SKMVIDTVITISLSYFSSTAPMIYTQHLSKALPEPSIDLK-NT 163
           H+ KR LE  FVH++S +   I  V      Y+S  A + Y  +++  L  P  DL+   
Sbjct: 141 HYFKRILETFFVHRFSHATSPIGNVFRNCAYYWSFGAYIAY--YVNHPLYTPVSDLQMKI 200

Query: 164 GIALFVIGITGNFYHHFLLSKTR-KQGETGYKIPKGGLFSIVICPHYLFEIIEFFGFAFI 223
           G    ++    NFY H LL   R   G  GY+IP+G LF+IV C +Y  EI ++ GF   
Sbjct: 201 GFGFGLVCQVANFYCHILLKNLRDPSGAGGYQIPRGFLFNIVTCANYTTEIYQWLGFNIA 260

Query: 224 SQTIYSLCF----AFATTFYLVGRSHATMR 247
           +QTI    F    A   T + +G+ H+ +R
Sbjct: 261 TQTIAGYVFLAVAALIMTNWALGK-HSRLR 287

[Close]

BLAST of Spg010133 vs. TAIR 10
Match: AT1G72590.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 48.1 bits (113), Expect = 1.3e-05
Identity = 52/212 (24.53%), Postives = 85/212 (40.09%), Query Frame = 0


Query: 93  RILLLKSALILHFLKRDLEVLFVHKYSSKMVIDTVITISLSYFSSTAPMIYTQHLSKALP 152
           R + L   + +H L+R +E  +V KYS+   +  +  +   ++   AP+    +++  + 
Sbjct: 112 RAVFLLLLMEIHVLRRVIESFYVFKYSTSARMHILAYVGALFYYVAAPLSLCSNIAPEVA 171

Query: 153 ---------------------------EPSIDLKN---TGIALFVIGITGNFYHHFLLSK 212
                                       P + L +    G A+F+ G       H +L  
Sbjct: 172 RFVGSQVAEFIASGKSHSHDFNLLLSISPLMKLGSLQWIGGAIFLWGWIHQRRCHAILGS 231

Query: 213 TRKQGETG--YKIPKGGLFSIVICPHYLFEIIEFFGFAFISQ----TIYSLC-FAFATTF 268
            R+       Y IP G  F +V CPH+L EI+ + G    S     +I+ L  F  A   
Sbjct: 232 LREYPSQAKEYIIPYGDWFEMVSCPHFLAEIVLYLGLLISSGGTDISIWLLFGFVAANLT 291

The following BLAST results are available for this feature:

BLAST of Spg010133 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6573901.1	9.9e-114	81.27	3-oxo-5-alpha-steroid 4-dehydrogenase 1, partial [Cucurbita argyrosperma subsp. ...	[more]
XP_022945516.1	9.9e-114	81.27	3-oxo-5-alpha-steroid 4-dehydrogenase 1-like [Cucurbita moschata]	[more]
XP_023542215.1	4.9e-113	80.90	3-oxo-5-alpha-steroid 4-dehydrogenase 1-like [Cucurbita pepo subsp. pepo]	[more]
XP_022968321.1	1.2e-111	79.40	3-oxo-5-alpha-steroid 4-dehydrogenase 1-like [Cucurbita maxima]	[more]
XP_038892898.1	1.1e-107	77.53	3-oxo-5-alpha-steroid 4-dehydrogenase 2-like [Benincasa hispida]	[more]

BLAST of Spg010133 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P18405	1.2e-16	41.51	3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Homo sapiens OX=9606 GN=SRD5A1 PE=1 S...	[more]
P24008	1.2e-16	41.51	3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Rattus norvegicus OX=10116 GN=Srd5a1 ...	[more]
Q28891	1.5e-16	28.57	3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Macaca fascicularis OX=9541 GN=SRD5A1...	[more]
Q68FF9	4.5e-16	40.57	3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Mus musculus OX=10090 GN=Srd5a1 PE=2 ...	[more]
Q28892	3.8e-15	39.62	3-oxo-5-alpha-steroid 4-dehydrogenase 2 OS=Macaca fascicularis OX=9541 GN=SRD5A2...	[more]

BLAST of Spg010133 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1G134	4.8e-114	81.27	3-oxo-5-alpha-steroid 4-dehydrogenase 1-like OS=Cucurbita moschata OX=3662 GN=LO...	[more]
A0A6J1HUJ4	5.9e-112	79.40	3-oxo-5-alpha-steroid 4-dehydrogenase 1-like OS=Cucurbita maxima OX=3661 GN=LOC1...	[more]
A0A6J1D974	1.1e-105	74.16	3-oxo-5-alpha-steroid 4-dehydrogenase 1-like OS=Momordica charantia OX=3673 GN=L...	[more]
A0A0A0KWK8	2.0e-104	73.51	S5A_REDUCTASE domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G6129...	[more]
A0A5D3CDA5	3.8e-103	73.51	3-oxo-5-alpha-steroid 4-dehydrogenase 1-like OS=Cucumis melo var. makuwa OX=1194...	[more]

BLAST of Spg010133 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G16010.1	9.4e-78	55.22	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]
AT2G38050.1	8.1e-13	35.51	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]
AT3G55360.1	9.9e-11	34.67	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]
AT1G72590.1	1.3e-05	24.53	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	1.20.120.1630		coord: 93..266 e-value: 3.3E-6 score: 28.9
None	No IPR available	PANTHER	PTHR10556:SF56	3-OXO-5-ALPHA-STEROID 4-DEHYDROGENASE 2-LIKE	coord: 4..267
IPR001104	3-oxo-5-alpha-steroid 4-dehydrogenase, C-terminal	PFAM	PF02544	Steroid_dh	coord: 158..267 e-value: 1.6E-28 score: 99.6
IPR001104	3-oxo-5-alpha-steroid 4-dehydrogenase, C-terminal	PROSITE	PS50244	S5A_REDUCTASE	coord: 162..242 score: 25.900263
IPR039357	3-oxo-5-alpha-steroid 4-dehydrogenase/very-long-chain enoyl-CoA reductase	PANTHER	PTHR10556	3-OXO-5-ALPHA-STEROID 4-DEHYDROGENASE	coord: 4..267

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg010133.1	Spg010133.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006629	lipid metabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0016627	oxidoreductase activity, acting on the CH-CH group of donors