Spg010085 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg010085
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Descriptionsigma factor binding protein 1, chloroplastic-like
Locationscaffold7: 6713957 .. 6714400 (-)
RNA-Seq ExpressionSpg010085
SyntenySpg010085
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATTTGATCAAGAGACAGCCGAGGAGAACCAAGAAATCGGCCGCCGATTTCAAAGTCGTGTACATTTCCAGTCCGATGAAGGTGAAAACCAGCGCCTCCAAGTTCCGATCTCTCGTCCAGAAACTCACCGGTCAGGACTCCGACGCCGAGAGGTTCATGGAACTCATCGGCGCCGCCGCCGGTGGTCGGAATGATAATCTCCAATCGTCGGCGTCGGCGTTCCTCGATTACCAACTCCTTGGCGACGAAGATTTAAGCGATCGCGATCATCGTCTTGTGGCGGGAAAGATGGAAACGGCGGCGTATCAGTCGTCGGTGGCTCCGGCGGAGGATTTGGTTTTGCAGAACTTCGATGCGAGTTTTGCGGAAATGTTCCATGGCTTTTTGCATGAATCTTCTACACAGTCTGAGATGCCTGCAGGTTTAAGTGCACTACTGTAA

mRNA sequence

ATGGATTTGATCAAGAGACAGCCGAGGAGAACCAAGAAATCGGCCGCCGATTTCAAAGTCGTGTACATTTCCAGTCCGATGAAGGTGAAAACCAGCGCCTCCAAGTTCCGATCTCTCGTCCAGAAACTCACCGGTCAGGACTCCGACGCCGAGAGGTTCATGGAACTCATCGGCGCCGCCGCCGGTGGTCGGAATGATAATCTCCAATCGTCGGCGTCGGCGTTCCTCGATTACCAACTCCTTGGCGACGAAGATTTAAGCGATCGCGATCATCGTCTTGTGGCGGGAAAGATGGAAACGGCGGCGTATCAGTCGTCGGTGGCTCCGGCGGAGGATTTGGTTTTGCAGAACTTCGATGCGAGTTTTGCGGAAATGTTCCATGGCTTTTTGCATGAATCTTCTACACAGTCTGAGATGCCTGCAGGTTTAAGTGCACTACTGTAA

Coding sequence (CDS)

ATGGATTTGATCAAGAGACAGCCGAGGAGAACCAAGAAATCGGCCGCCGATTTCAAAGTCGTGTACATTTCCAGTCCGATGAAGGTGAAAACCAGCGCCTCCAAGTTCCGATCTCTCGTCCAGAAACTCACCGGTCAGGACTCCGACGCCGAGAGGTTCATGGAACTCATCGGCGCCGCCGCCGGTGGTCGGAATGATAATCTCCAATCGTCGGCGTCGGCGTTCCTCGATTACCAACTCCTTGGCGACGAAGATTTAAGCGATCGCGATCATCGTCTTGTGGCGGGAAAGATGGAAACGGCGGCGTATCAGTCGTCGGTGGCTCCGGCGGAGGATTTGGTTTTGCAGAACTTCGATGCGAGTTTTGCGGAAATGTTCCATGGCTTTTTGCATGAATCTTCTACACAGTCTGAGATGCCTGCAGGTTTAAGTGCACTACTGTAA

Protein sequence

MDLIKRQPRRTKKSAADFKVVYISSPMKVKTSASKFRSLVQKLTGQDSDAERFMELIGAAAGGRNDNLQSSASAFLDYQLLGDEDLSDRDHRLVAGKMETAAYQSSVAPAEDLVLQNFDASFAEMFHGFLHESSTQSEMPAGLSALL
Homology
BLAST of Spg010085 vs. NCBI nr
Match: XP_023542375.1 (sigma factor binding protein 1, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 207.2 bits (526), Expect = 9.5e-50
Identity = 121/153 (79.08%), Postives = 132/153 (86.27%), Query Frame = 0

Query: 1   MDLI--KRQPRRTKKS-AADFKVVYISSPMKVKTSASKFRSLVQKLTGQDSDAERFMELI 60
           MDL   K+  RR++KS AADFKVVYIS+PMKVKTSAS+FRSLVQKLTG DSDAERFMELI
Sbjct: 1   MDLTAKKQSKRRSRKSAAADFKVVYISNPMKVKTSASRFRSLVQKLTGLDSDAERFMELI 60

Query: 61  GAAAGGRNDNLQS--SASAFLDYQLLGDEDLSDRDHRLVAGKMETAAYQSSVAPAEDLVL 120
            AAA    +NLQS  SASAFLDYQLLGD+DL+D DHRLVAGK+ETAAYQSSVAP EDLV+
Sbjct: 61  AAAASSDRNNLQSSTSASAFLDYQLLGDDDLTDCDHRLVAGKIETAAYQSSVAPPEDLVM 120

Query: 121 QNFDASFAEMFHGFLHE-SSTQSEMPAGLSALL 148
           QNFDASFAEMF  FLHE SSTQ+EMP G SALL
Sbjct: 121 QNFDASFAEMFQSFLHESSSTQTEMP-GFSALL 152

BLAST of Spg010085 vs. NCBI nr
Match: XP_022966872.1 (sigma factor binding protein 1, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 207.2 bits (526), Expect = 9.5e-50
Identity = 121/153 (79.08%), Postives = 132/153 (86.27%), Query Frame = 0

Query: 1   MDLIKRQ--PRRTKKS-AADFKVVYISSPMKVKTSASKFRSLVQKLTGQDSDAERFMELI 60
           MDL  +Q   RR++KS AADFKVVYIS+PMKVKTSAS+FRSLVQKLTG DSDAERFMELI
Sbjct: 1   MDLTAKQQSKRRSRKSAAADFKVVYISNPMKVKTSASRFRSLVQKLTGLDSDAERFMELI 60

Query: 61  GAAAGGRNDNLQS--SASAFLDYQLLGDEDLSDRDHRLVAGKMETAAYQSSVAPAEDLVL 120
            AAA    +NLQS  SASAFLDYQLLGD+DL+D DHRLVAGK+ETAAYQSSVAP EDLV+
Sbjct: 61  AAAASSDRNNLQSSTSASAFLDYQLLGDDDLTDCDHRLVAGKIETAAYQSSVAPPEDLVM 120

Query: 121 QNFDASFAEMFHGFLHE-SSTQSEMPAGLSALL 148
           QNFDASFAEMF  FLHE SSTQ+EMP G SALL
Sbjct: 121 QNFDASFAEMFQSFLHESSSTQTEMP-GFSALL 152

BLAST of Spg010085 vs. NCBI nr
Match: KAG6573793.1 (Sigma factor binding protein 1, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 204.9 bits (520), Expect = 4.7e-49
Identity = 119/153 (77.78%), Postives = 132/153 (86.27%), Query Frame = 0

Query: 1   MDLI--KRQPRRTKKS-AADFKVVYISSPMKVKTSASKFRSLVQKLTGQDSDAERFMELI 60
           MDL   K+  RR++KS AADFKVVYIS+PMKVKTSAS+FRSLVQKLTG DSDAERFMELI
Sbjct: 1   MDLTAKKQSKRRSRKSAAADFKVVYISNPMKVKTSASRFRSLVQKLTGLDSDAERFMELI 60

Query: 61  GAAAGGRNDNLQS--SASAFLDYQLLGDEDLSDRDHRLVAGKMETAAYQSSVAPAEDLVL 120
            AAA    +NLQS  SASAFLDYQLLGD+DL+D DHRLVAGK+ETAAYQSSVAP EDLV+
Sbjct: 61  AAAASSDRNNLQSSTSASAFLDYQLLGDDDLTDCDHRLVAGKIETAAYQSSVAPPEDLVM 120

Query: 121 QNFDASFAEMFHGFLHE-SSTQSEMPAGLSALL 148
           +NFDASFAEMF  FLH+ SSTQ+EMP G SALL
Sbjct: 121 ENFDASFAEMFQSFLHDSSSTQTEMP-GFSALL 152

BLAST of Spg010085 vs. NCBI nr
Match: XP_022945563.1 (sigma factor binding protein 1, chloroplastic-like [Cucurbita moschata] >KAG7012870.1 Sigma factor binding protein 1, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 204.9 bits (520), Expect = 4.7e-49
Identity = 119/153 (77.78%), Postives = 132/153 (86.27%), Query Frame = 0

Query: 1   MDLI--KRQPRRTKKS-AADFKVVYISSPMKVKTSASKFRSLVQKLTGQDSDAERFMELI 60
           MDL   K+  RR++KS AADFKVVYIS+PMKVKTSAS+FRSLVQKLTG DSDAERFMELI
Sbjct: 1   MDLTAKKQSKRRSRKSAAADFKVVYISNPMKVKTSASRFRSLVQKLTGLDSDAERFMELI 60

Query: 61  GAAAGGRNDNLQS--SASAFLDYQLLGDEDLSDRDHRLVAGKMETAAYQSSVAPAEDLVL 120
            AAA    +NLQS  SASAFLDYQLLGD+DL+D DHRLVAGK+ETAAYQSSVAP EDLV+
Sbjct: 61  AAAASSDRNNLQSSTSASAFLDYQLLGDDDLTDCDHRLVAGKIETAAYQSSVAPPEDLVM 120

Query: 121 QNFDASFAEMFHGFLHE-SSTQSEMPAGLSALL 148
           +NFDASFAEMF  FLH+ SSTQ+EMP G SALL
Sbjct: 121 ENFDASFAEMFQSFLHDSSSTQTEMP-GFSALL 152

BLAST of Spg010085 vs. NCBI nr
Match: XP_022139181.1 (uncharacterized protein LOC111010149 [Momordica charantia])

HSP 1 Score: 188.3 bits (477), Expect = 4.6e-44
Identity = 109/154 (70.78%), Postives = 131/154 (85.06%), Query Frame = 0

Query: 1   MDLIKRQP---RRTKKSAADFKVVYISSPMKVKTSASKFRSLVQKLTGQDSDAERFMELI 60
           MD+I R+    RR+KKSAADFKVVYISSPMKVKTSASKFRSLVQKLTGQDSDAERFMELI
Sbjct: 1   MDVIGRKQAKIRRSKKSAADFKVVYISSPMKVKTSASKFRSLVQKLTGQDSDAERFMELI 60

Query: 61  GAAAGGRNDNLQ-SSASAFLDYQLL--GDEDLSDRD--HRLVAGKMETAAYQSSVAPAED 120
            AAA    ++LQ SS++AFLD+QLL   D+D  DRD  HRLV+G +E ++YQSS+AP +D
Sbjct: 61  AAAAAADRNHLQPSSSAAFLDHQLLCDADDDQIDRDNHHRLVSGNIE-SSYQSSIAPPDD 120

Query: 121 LVLQNFDASFAEMFHGFLHESSTQSEMPAGLSAL 147
           L+LQNFDA+FA+MFHGFLH+SSTQ++MP GL+ L
Sbjct: 121 LILQNFDANFADMFHGFLHDSSTQTQMP-GLALL 152

BLAST of Spg010085 vs. ExPASy Swiss-Prot
Match: Q9LDH1 (Sigma factor binding protein 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=SIB1 PE=1 SV=1)

HSP 1 Score: 48.5 bits (114), Expect = 7.4e-05
Identity = 24/43 (55.81%), Postives = 33/43 (76.74%), Query Frame = 0

Query: 6  RQPRRTKKSAADFKVVYISSPMKVKTSASKFRSLVQKLTGQDS 49
          +Q ++T  +    KV YIS+PM+V+T ASKFR LVQ+LTGQD+
Sbjct: 28 KQKKKTTSTNKPIKVRYISNPMRVQTCASKFRELVQELTGQDA 70

BLAST of Spg010085 vs. ExPASy Swiss-Prot
Match: O80669 (Sigma factor binding protein 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=SIB2 PE=1 SV=1)

HSP 1 Score: 45.8 bits (107), Expect = 4.8e-04
Identity = 23/43 (53.49%), Postives = 32/43 (74.42%), Query Frame = 0

Query: 6  RQPRRTKKSAADFKVVYISSPMKVKTSASKFRSLVQKLTGQDS 49
          +Q R++  +    KV YIS+PM+V+T  SKFR LVQ+LTGQD+
Sbjct: 25 KQKRKSTTTHKPIKVRYISNPMRVETCPSKFRELVQELTGQDA 67

BLAST of Spg010085 vs. ExPASy TrEMBL
Match: A0A6J1HP65 (sigma factor binding protein 1, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111466446 PE=4 SV=1)

HSP 1 Score: 207.2 bits (526), Expect = 4.6e-50
Identity = 121/153 (79.08%), Postives = 132/153 (86.27%), Query Frame = 0

Query: 1   MDLIKRQ--PRRTKKS-AADFKVVYISSPMKVKTSASKFRSLVQKLTGQDSDAERFMELI 60
           MDL  +Q   RR++KS AADFKVVYIS+PMKVKTSAS+FRSLVQKLTG DSDAERFMELI
Sbjct: 1   MDLTAKQQSKRRSRKSAAADFKVVYISNPMKVKTSASRFRSLVQKLTGLDSDAERFMELI 60

Query: 61  GAAAGGRNDNLQS--SASAFLDYQLLGDEDLSDRDHRLVAGKMETAAYQSSVAPAEDLVL 120
            AAA    +NLQS  SASAFLDYQLLGD+DL+D DHRLVAGK+ETAAYQSSVAP EDLV+
Sbjct: 61  AAAASSDRNNLQSSTSASAFLDYQLLGDDDLTDCDHRLVAGKIETAAYQSSVAPPEDLVM 120

Query: 121 QNFDASFAEMFHGFLHE-SSTQSEMPAGLSALL 148
           QNFDASFAEMF  FLHE SSTQ+EMP G SALL
Sbjct: 121 QNFDASFAEMFQSFLHESSSTQTEMP-GFSALL 152

BLAST of Spg010085 vs. ExPASy TrEMBL
Match: A0A6J1G190 (sigma factor binding protein 1, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111449759 PE=4 SV=1)

HSP 1 Score: 204.9 bits (520), Expect = 2.3e-49
Identity = 119/153 (77.78%), Postives = 132/153 (86.27%), Query Frame = 0

Query: 1   MDLI--KRQPRRTKKS-AADFKVVYISSPMKVKTSASKFRSLVQKLTGQDSDAERFMELI 60
           MDL   K+  RR++KS AADFKVVYIS+PMKVKTSAS+FRSLVQKLTG DSDAERFMELI
Sbjct: 1   MDLTAKKQSKRRSRKSAAADFKVVYISNPMKVKTSASRFRSLVQKLTGLDSDAERFMELI 60

Query: 61  GAAAGGRNDNLQS--SASAFLDYQLLGDEDLSDRDHRLVAGKMETAAYQSSVAPAEDLVL 120
            AAA    +NLQS  SASAFLDYQLLGD+DL+D DHRLVAGK+ETAAYQSSVAP EDLV+
Sbjct: 61  AAAASSDRNNLQSSTSASAFLDYQLLGDDDLTDCDHRLVAGKIETAAYQSSVAPPEDLVM 120

Query: 121 QNFDASFAEMFHGFLHE-SSTQSEMPAGLSALL 148
           +NFDASFAEMF  FLH+ SSTQ+EMP G SALL
Sbjct: 121 ENFDASFAEMFQSFLHDSSSTQTEMP-GFSALL 152

BLAST of Spg010085 vs. ExPASy TrEMBL
Match: A0A6J1CBX0 (uncharacterized protein LOC111010149 OS=Momordica charantia OX=3673 GN=LOC111010149 PE=4 SV=1)

HSP 1 Score: 188.3 bits (477), Expect = 2.2e-44
Identity = 109/154 (70.78%), Postives = 131/154 (85.06%), Query Frame = 0

Query: 1   MDLIKRQP---RRTKKSAADFKVVYISSPMKVKTSASKFRSLVQKLTGQDSDAERFMELI 60
           MD+I R+    RR+KKSAADFKVVYISSPMKVKTSASKFRSLVQKLTGQDSDAERFMELI
Sbjct: 1   MDVIGRKQAKIRRSKKSAADFKVVYISSPMKVKTSASKFRSLVQKLTGQDSDAERFMELI 60

Query: 61  GAAAGGRNDNLQ-SSASAFLDYQLL--GDEDLSDRD--HRLVAGKMETAAYQSSVAPAED 120
            AAA    ++LQ SS++AFLD+QLL   D+D  DRD  HRLV+G +E ++YQSS+AP +D
Sbjct: 61  AAAAAADRNHLQPSSSAAFLDHQLLCDADDDQIDRDNHHRLVSGNIE-SSYQSSIAPPDD 120

Query: 121 LVLQNFDASFAEMFHGFLHESSTQSEMPAGLSAL 147
           L+LQNFDA+FA+MFHGFLH+SSTQ++MP GL+ L
Sbjct: 121 LILQNFDANFADMFHGFLHDSSTQTQMP-GLALL 152

BLAST of Spg010085 vs. ExPASy TrEMBL
Match: A0A6J1GZT9 (sigma factor binding protein 1, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111458651 PE=4 SV=1)

HSP 1 Score: 177.2 bits (448), Expect = 5.1e-41
Identity = 102/131 (77.86%), Postives = 110/131 (83.97%), Query Frame = 0

Query: 1   MDLI-KRQPRRTKKSAADFKVVYISSPMKVKTSASKFRSLVQKLTGQDSDAERFMELIGA 60
           MD+I K+ P+RTKKSAA FKVVYISSPMKVKTSASKFRSLVQKLTGQDSDAE FMELI A
Sbjct: 1   MDVIGKKPPKRTKKSAAGFKVVYISSPMKVKTSASKFRSLVQKLTGQDSDAESFMELIAA 60

Query: 61  AAGGRNDNLQSSASAFLDYQLLGDEDLSDRDHRLVAGKMETAAYQSSVAPAE-DLVLQNF 120
                + N QS  SAFLDYQ+LGD++L DRDHRLVAGK+ETAAYQS VAP E D VLQNF
Sbjct: 61  -----DRNFQS--SAFLDYQILGDDNLIDRDHRLVAGKIETAAYQSLVAPPEDDFVLQNF 120

Query: 121 DASFAEMFHGF 130
           DASF EM HGF
Sbjct: 121 DASFEEMLHGF 124

BLAST of Spg010085 vs. ExPASy TrEMBL
Match: A0A6J1JD32 (uncharacterized protein LOC111483942 OS=Cucurbita maxima OX=3661 GN=LOC111483942 PE=4 SV=1)

HSP 1 Score: 176.8 bits (447), Expect = 6.7e-41
Identity = 101/131 (77.10%), Postives = 113/131 (86.26%), Query Frame = 0

Query: 1   MDLI-KRQPRRTKKSAADFKVVYISSPMKVKTSASKFRSLVQKLTGQDSDAERFMELIGA 60
           MD+I K+QP+RTKKSAA+FKVVYISSPMKVKTSASKFRSLVQKLTGQDSDAE F+ELI  
Sbjct: 1   MDVIGKKQPKRTKKSAANFKVVYISSPMKVKTSASKFRSLVQKLTGQDSDAESFLELI-- 60

Query: 61  AAGGRNDNLQSSASAFLDYQLLGDEDLSDRDHRLVAGKMETAAYQSSVA-PAEDLVLQNF 120
            AG RN      +SAFLDYQ+LGD++L +RDHRLVAGK+ETAAYQS VA PA+DLVLQNF
Sbjct: 61  -AGDRN----FQSSAFLDYQILGDDNLINRDHRLVAGKIETAAYQSLVAPPADDLVLQNF 120

Query: 121 DASFAEMFHGF 130
           DASF EM  GF
Sbjct: 121 DASFEEMLRGF 124

BLAST of Spg010085 vs. TAIR 10
Match: AT3G56710.1 (sigma factor binding protein 1 )

HSP 1 Score: 48.5 bits (114), Expect = 5.3e-06
Identity = 24/43 (55.81%), Postives = 33/43 (76.74%), Query Frame = 0

Query: 6  RQPRRTKKSAADFKVVYISSPMKVKTSASKFRSLVQKLTGQDS 49
          +Q ++T  +    KV YIS+PM+V+T ASKFR LVQ+LTGQD+
Sbjct: 28 KQKKKTTSTNKPIKVRYISNPMRVQTCASKFRELVQELTGQDA 70

BLAST of Spg010085 vs. TAIR 10
Match: AT2G41180.1 (VQ motif-containing protein )

HSP 1 Score: 45.8 bits (107), Expect = 3.4e-05
Identity = 23/43 (53.49%), Postives = 32/43 (74.42%), Query Frame = 0

Query: 6  RQPRRTKKSAADFKVVYISSPMKVKTSASKFRSLVQKLTGQDS 49
          +Q R++  +    KV YIS+PM+V+T  SKFR LVQ+LTGQD+
Sbjct: 25 KQKRKSTTTHKPIKVRYISNPMRVETCPSKFRELVQELTGQDA 67

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023542375.19.5e-5079.08sigma factor binding protein 1, chloroplastic-like [Cucurbita pepo subsp. pepo][more]
XP_022966872.19.5e-5079.08sigma factor binding protein 1, chloroplastic-like [Cucurbita maxima][more]
KAG6573793.14.7e-4977.78Sigma factor binding protein 1, chloroplastic, partial [Cucurbita argyrosperma s... [more]
XP_022945563.14.7e-4977.78sigma factor binding protein 1, chloroplastic-like [Cucurbita moschata] >KAG7012... [more]
XP_022139181.14.6e-4470.78uncharacterized protein LOC111010149 [Momordica charantia][more]
Match NameE-valueIdentityDescription
Q9LDH17.4e-0555.81Sigma factor binding protein 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN... [more]
O806694.8e-0453.49Sigma factor binding protein 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN... [more]
Match NameE-valueIdentityDescription
A0A6J1HP654.6e-5079.08sigma factor binding protein 1, chloroplastic-like OS=Cucurbita maxima OX=3661 G... [more]
A0A6J1G1902.3e-4977.78sigma factor binding protein 1, chloroplastic-like OS=Cucurbita moschata OX=3662... [more]
A0A6J1CBX02.2e-4470.78uncharacterized protein LOC111010149 OS=Momordica charantia OX=3673 GN=LOC111010... [more]
A0A6J1GZT95.1e-4177.86sigma factor binding protein 1, chloroplastic-like OS=Cucurbita moschata OX=3662... [more]
A0A6J1JD326.7e-4177.10uncharacterized protein LOC111483942 OS=Cucurbita maxima OX=3661 GN=LOC111483942... [more]
Match NameE-valueIdentityDescription
AT3G56710.15.3e-0655.81sigma factor binding protein 1 [more]
AT2G41180.13.4e-0553.49VQ motif-containing protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR008889VQPFAMPF05678VQcoord: 23..49
e-value: 4.8E-12
score: 45.1
IPR039335Sigma factor binding protein 1/2PANTHERPTHR33624SIGMA FACTOR BINDING PROTEIN 1, CHLOROPLASTICcoord: 7..125
NoneNo IPR availablePANTHERPTHR33624:SF14OS07G0687400 PROTEINcoord: 7..125

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg010085.1Spg010085.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0051091 positive regulation of DNA-binding transcription factor activity