Spg010056 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg010056 vs. NCBI nr
Match: XP_038891534.1 (uncharacterized protein LOC120080925 [Benincasa hispida] >XP_038891535.1 uncharacterized protein LOC120080925 [Benincasa hispida])

HSP 1 Score: 647.1 bits (1668), Expect = 9.9e-182
Identity = 329/376 (87.50%), Postives = 353/376 (93.88%), Query Frame = 0

Query: 30  RFSDEFR-KPGRCVFEEKFIARDSGTLEQLKEMTSRRRVIEESINETSSITDAIAREMYG 89
           +FSD  + K   CVFE+KF+ARDSGTLEQLKEMTSRRRVIEESINETSSITDAIAREM G
Sbjct: 4   QFSDPAKLKTKMCVFEDKFLARDSGTLEQLKEMTSRRRVIEESINETSSITDAIAREMSG 63

Query: 90  GLSSHTQQVLHKLQQYLPLLENFVSQVDLVDYKLKLVQWTSELKIRWSSALSSSSLFQLR 149
           GLSSHTQQVLHKLQ+YLPLLENFVSQVD++DY L++VQWTS+LKIRWSSALSSSSLF LR
Sbjct: 64  GLSSHTQQVLHKLQKYLPLLENFVSQVDMIDYNLRIVQWTSDLKIRWSSALSSSSLFHLR 123

Query: 150 GPKFFQIDNLRFEVGMILFLYGAVLRQRAVEVMSEDIVQSATFFREAAGVYQYLAKEILP 209
           GPKFFQIDNLRFEVGM L LYGAVLRQRA EV+SED VQSATFFREAAG+YQYLAKEILP
Sbjct: 124 GPKFFQIDNLRFEVGMTLSLYGAVLRQRASEVISEDQVQSATFFREAAGIYQYLAKEILP 183

Query: 210 SIQHCLPSEKPPEVIPSTSVAMSLVCLAEAQAVTTMRAEEKGTISSLLAKLHYGIVDLLN 269
           SIQHCLPSE+PPEVIPSTS AMSL+CLAEAQAVT+MRAEEKGTI SLLAKLHYGIV+LLN
Sbjct: 184 SIQHCLPSERPPEVIPSTSAAMSLICLAEAQAVTSMRAEEKGTIPSLLAKLHYGIVELLN 243

Query: 270 ESANHLRIISGECKDISSNFLEFLSAFRALHELKCWKNIAKDLMSGGHAGVAIGVLRHAL 329
           ES NHLRIISGE KD+SSNFLEFLSAFRALHELK  KN+AK+LMSGG AGVAIGVLR+AL
Sbjct: 244 ESVNHLRIISGEWKDMSSNFLEFLSAFRALHELKSRKNLAKELMSGGQAGVAIGVLRYAL 303

Query: 330 TDVKKEMPRENSWKLIFGTEIDIVAETLRKFERENEIVWHKKIPSRDELPTPKGSQIVKS 389
           TDVKKEMPRENSWK++FG EIDIVAE LRKFERENEIVWH KIPSRDELPTPKG+QIVKS
Sbjct: 304 TDVKKEMPRENSWKIVFGIEIDIVAEMLRKFERENEIVWHNKIPSRDELPTPKGTQIVKS 363

Query: 390 IPYHPEKWERELAFKI 405
           IPYHP+KWERELAFKI
Sbjct: 364 IPYHPKKWERELAFKI 379

BLAST of Spg010056 vs. NCBI nr
Match: XP_023541232.1 (uncharacterized protein LOC111801459 [Cucurbita pepo subsp. pepo] >XP_023541233.1 uncharacterized protein LOC111801459 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 638.6 bits (1646), Expect = 3.5e-179
Identity = 331/376 (88.03%), Postives = 352/376 (93.62%), Query Frame = 0

Query: 30  RFSDEFR-KPGRCVFEEKFIARDSGTLEQLKEMTSRRRVIEESINETSSITDAIAREMYG 89
           +FSD  + K   CVFE+KFIARDS TLEQLKEMTSRRRVIEESINETSSITDAIAREM G
Sbjct: 4   QFSDPAKLKTKMCVFEDKFIARDSSTLEQLKEMTSRRRVIEESINETSSITDAIAREMSG 63

Query: 90  GLSSHTQQVLHKLQQYLPLLENFVSQVDLVDYKLKLVQWTSELKIRWSSALSSSSLFQLR 149
           GLSSHTQQVLHKL QYLPLLENFVSQVDLV Y LK+VQWTS+LKIRWSSALSSSSLF LR
Sbjct: 64  GLSSHTQQVLHKLLQYLPLLENFVSQVDLVGYNLKIVQWTSDLKIRWSSALSSSSLFHLR 123

Query: 150 GPKFFQIDNLRFEVGMILFLYGAVLRQRAVEVMSEDIVQSATFFREAAGVYQYLAKEILP 209
           GPKFFQIDNLRFEV M LFLYGAVLRQRA+EV+SED VQSATFFREAAGVYQYLAKEILP
Sbjct: 124 GPKFFQIDNLRFEVAMTLFLYGAVLRQRALEVISEDQVQSATFFREAAGVYQYLAKEILP 183

Query: 210 SIQHCLPSEKPPEVIPSTSVAMSLVCLAEAQAVTTMRAEEKGTISSLLAKLHYGIVDLLN 269
           SIQHC+PSE+PPEVIPSTSVAMSLVCLAEAQAVTTMRAEEKG+ISSLLAKLHYGIVDLLN
Sbjct: 184 SIQHCVPSERPPEVIPSTSVAMSLVCLAEAQAVTTMRAEEKGSISSLLAKLHYGIVDLLN 243

Query: 270 ESANHLRIISGECKDISSNFLEFLSAFRALHELKCWKNIAKDLMSGGHAGVAIGVLRHAL 329
           ESANHL+I+SGE    SS+FLEFLSAFRALHELK  KN+AK+LMSGG AGVAIG+LRHAL
Sbjct: 244 ESANHLQILSGE----SSSFLEFLSAFRALHELKSRKNLAKELMSGGQAGVAIGILRHAL 303

Query: 330 TDVKKEMPRENSWKLIFGTEIDIVAETLRKFERENEIVWHKKIPSRDELPTPKGSQIVKS 389
           TDVKK+MPRENSW+L+FGTEID VAETLRKFERENEIVWHKKIPSRDELPTPKGSQIVKS
Sbjct: 304 TDVKKDMPRENSWRLVFGTEIDSVAETLRKFERENEIVWHKKIPSRDELPTPKGSQIVKS 363

Query: 390 IPYHPEKWERELAFKI 405
           IPY P+KWERELAFK+
Sbjct: 364 IPYCPKKWERELAFKM 375

BLAST of Spg010056 vs. NCBI nr
Match: XP_022944843.1 (uncharacterized protein LOC111449253 [Cucurbita moschata] >XP_022944844.1 uncharacterized protein LOC111449253 [Cucurbita moschata])

HSP 1 Score: 637.1 bits (1642), Expect = 1.0e-178
Identity = 330/376 (87.77%), Postives = 351/376 (93.35%), Query Frame = 0

Query: 30  RFSDEFR-KPGRCVFEEKFIARDSGTLEQLKEMTSRRRVIEESINETSSITDAIAREMYG 89
           +FSD  + K   CVFE+KFIARDS TLEQLKEMTSRRRVIEESINETSSITDAIAREM G
Sbjct: 4   QFSDPAKLKTKMCVFEDKFIARDSSTLEQLKEMTSRRRVIEESINETSSITDAIAREMSG 63

Query: 90  GLSSHTQQVLHKLQQYLPLLENFVSQVDLVDYKLKLVQWTSELKIRWSSALSSSSLFQLR 149
           GLSSHTQQVLHKL QYLPLLENFVSQVDLV Y LK+VQWTS+LKIRWSSALSSSSLF LR
Sbjct: 64  GLSSHTQQVLHKLLQYLPLLENFVSQVDLVGYNLKIVQWTSDLKIRWSSALSSSSLFHLR 123

Query: 150 GPKFFQIDNLRFEVGMILFLYGAVLRQRAVEVMSEDIVQSATFFREAAGVYQYLAKEILP 209
           GPKFFQIDNLRFEV M LFLYGAVLRQRA+EV+SED VQSATFFREAAGVYQYLAK+ILP
Sbjct: 124 GPKFFQIDNLRFEVAMTLFLYGAVLRQRALEVISEDQVQSATFFREAAGVYQYLAKDILP 183

Query: 210 SIQHCLPSEKPPEVIPSTSVAMSLVCLAEAQAVTTMRAEEKGTISSLLAKLHYGIVDLLN 269
           SIQHC+PSE+PPEVIPSTSVAMSLVCLAEAQAVTTMRAEEKG+ISSLLAKLHYGIVDLLN
Sbjct: 184 SIQHCVPSERPPEVIPSTSVAMSLVCLAEAQAVTTMRAEEKGSISSLLAKLHYGIVDLLN 243

Query: 270 ESANHLRIISGECKDISSNFLEFLSAFRALHELKCWKNIAKDLMSGGHAGVAIGVLRHAL 329
           ESANHLRI+SGE    SS+FLEFLSAFRALHELK  KN+AK+LMSGG AGVAIG+LRHAL
Sbjct: 244 ESANHLRILSGE----SSSFLEFLSAFRALHELKSRKNLAKELMSGGQAGVAIGILRHAL 303

Query: 330 TDVKKEMPRENSWKLIFGTEIDIVAETLRKFERENEIVWHKKIPSRDELPTPKGSQIVKS 389
           TDVKK+MPRENSW+L+FGTEID VAETLRKFERENEIVWHKKIPSRDELPTPKGSQIV S
Sbjct: 304 TDVKKDMPRENSWRLVFGTEIDSVAETLRKFERENEIVWHKKIPSRDELPTPKGSQIVSS 363

Query: 390 IPYHPEKWERELAFKI 405
           IPY P+KWERELAFK+
Sbjct: 364 IPYCPKKWERELAFKM 375

BLAST of Spg010056 vs. NCBI nr
Match: XP_008446562.1 (PREDICTED: uncharacterized protein LOC103489257 [Cucumis melo])

HSP 1 Score: 634.8 bits (1636), Expect = 5.1e-178
Identity = 323/376 (85.90%), Postives = 351/376 (93.35%), Query Frame = 0

Query: 30  RFSDEFR-KPGRCVFEEKFIARDSGTLEQLKEMTSRRRVIEESINETSSITDAIAREMYG 89
           +FSD  + K   CVFE+KFIARDSGTLEQLKEMTSRRRVIEESINETSSITDAIAREM G
Sbjct: 4   QFSDPSKLKTKMCVFEDKFIARDSGTLEQLKEMTSRRRVIEESINETSSITDAIAREMSG 63

Query: 90  GLSSHTQQVLHKLQQYLPLLENFVSQVDLVDYKLKLVQWTSELKIRWSSALSSSSLFQLR 149
           GLSSHTQQVLHKLQQYLPLLENF+SQVD++DY L++VQWTS+LKIRWSSALSSS LF LR
Sbjct: 64  GLSSHTQQVLHKLQQYLPLLENFISQVDMLDYNLRIVQWTSDLKIRWSSALSSSLLFHLR 123

Query: 150 GPKFFQIDNLRFEVGMILFLYGAVLRQRAVEVMSEDIVQSATFFREAAGVYQYLAKEILP 209
           GPKFFQIDNLRFEVGM+L LYGA LRQRA+EV+SED VQSATFFREAAGVYQYLA+EILP
Sbjct: 124 GPKFFQIDNLRFEVGMMLSLYGAFLRQRALEVISEDQVQSATFFREAAGVYQYLAEEILP 183

Query: 210 SIQHCLPSEKPPEVIPSTSVAMSLVCLAEAQAVTTMRAEEKGTISSLLAKLHYGIVDLLN 269
           +IQHCLPSE+PPEVIPSTS AMSL+CLAEAQAVT+MRAEEKGTI SLLAKLHYGIV+LLN
Sbjct: 184 TIQHCLPSERPPEVIPSTSAAMSLICLAEAQAVTSMRAEEKGTIPSLLAKLHYGIVELLN 243

Query: 270 ESANHLRIISGECKDISSNFLEFLSAFRALHELKCWKNIAKDLMSGGHAGVAIGVLRHAL 329
           ESANHL I SGE KD+SSNFLEFLSAFRALHEL   KN+AK+LMSGG +GVAIGVLR+AL
Sbjct: 244 ESANHLHISSGESKDMSSNFLEFLSAFRALHELMSRKNLAKELMSGGQSGVAIGVLRYAL 303

Query: 330 TDVKKEMPRENSWKLIFGTEIDIVAETLRKFERENEIVWHKKIPSRDELPTPKGSQIVKS 389
           TDVKKEMPRENSWKL+FG EIDIV ETLRKFERENEIVWHKKIPSRDELPTP G+QIVKS
Sbjct: 304 TDVKKEMPRENSWKLVFGIEIDIVTETLRKFERENEIVWHKKIPSRDELPTPVGTQIVKS 363

Query: 390 IPYHPEKWERELAFKI 405
           IPY+P+KWER+LAFKI
Sbjct: 364 IPYNPKKWERDLAFKI 379

BLAST of Spg010056 vs. NCBI nr
Match: KAG6573989.1 (hypothetical protein SDJN03_27876, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 634.0 bits (1634), Expect = 8.7e-178
Identity = 329/376 (87.50%), Postives = 350/376 (93.09%), Query Frame = 0

Query: 30  RFSDEFR-KPGRCVFEEKFIARDSGTLEQLKEMTSRRRVIEESINETSSITDAIAREMYG 89
           +FSD  + K   CVFE+KFIARDS TLEQLKEMTSRRRVIEESINETSSITDAIAREM G
Sbjct: 4   QFSDPAKLKTKMCVFEDKFIARDSSTLEQLKEMTSRRRVIEESINETSSITDAIAREMSG 63

Query: 90  GLSSHTQQVLHKLQQYLPLLENFVSQVDLVDYKLKLVQWTSELKIRWSSALSSSSLFQLR 149
           GLSSHTQQVLHKL QYLPLLENFVSQVDLV Y LK+VQWTS+LKIRWSSALSSSSLF LR
Sbjct: 64  GLSSHTQQVLHKLLQYLPLLENFVSQVDLVGYNLKIVQWTSDLKIRWSSALSSSSLFHLR 123

Query: 150 GPKFFQIDNLRFEVGMILFLYGAVLRQRAVEVMSEDIVQSATFFREAAGVYQYLAKEILP 209
           GPKF QIDNLRFEV M LFLYGAVLRQRA+EV+SED VQSATFFREAAGVYQYLAK+ILP
Sbjct: 124 GPKFVQIDNLRFEVAMTLFLYGAVLRQRALEVISEDQVQSATFFREAAGVYQYLAKDILP 183

Query: 210 SIQHCLPSEKPPEVIPSTSVAMSLVCLAEAQAVTTMRAEEKGTISSLLAKLHYGIVDLLN 269
           SIQHC+PSE+PPEVIPSTSVAMSLVCLAEAQAVTTMRAEEKG+ISSLLAKLHYGIVDLLN
Sbjct: 184 SIQHCVPSERPPEVIPSTSVAMSLVCLAEAQAVTTMRAEEKGSISSLLAKLHYGIVDLLN 243

Query: 270 ESANHLRIISGECKDISSNFLEFLSAFRALHELKCWKNIAKDLMSGGHAGVAIGVLRHAL 329
           ESANHLRI+SGE    SS+FLEFLSAFRALHELK  KN+AK+LMSGG AGVAIG+LRHAL
Sbjct: 244 ESANHLRILSGE----SSSFLEFLSAFRALHELKSRKNLAKELMSGGQAGVAIGILRHAL 303

Query: 330 TDVKKEMPRENSWKLIFGTEIDIVAETLRKFERENEIVWHKKIPSRDELPTPKGSQIVKS 389
           TDVKK+MPRENSW+L+FGTEID VAETLRKFERENEIVWHKKIPSRDELPTPKGSQIV S
Sbjct: 304 TDVKKDMPRENSWRLVFGTEIDSVAETLRKFERENEIVWHKKIPSRDELPTPKGSQIVTS 363

Query: 390 IPYHPEKWERELAFKI 405
           IPY P+KWERELAFK+
Sbjct: 364 IPYCPKKWERELAFKM 375

BLAST of Spg010056 vs. ExPASy Swiss-Prot
Match: Q9W6C5 (Programmed cell death 6-interacting protein OS=Xenopus laevis OX=8355 GN=pdcd6ip PE=1 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 8.0e-09
Identity = 65/299 (21.74%), Postives = 127/299 (42.47%), Query Frame = 0

Query: 98  LHKLQQYLPLLENFVSQVDLVDYKLKLVQWTSELKIRWSSALSSSSLFQLRGPKFFQIDN 157
           L K +  L  +  +  Q+  V+ K    +    L   W  A    S+F   G     + +
Sbjct: 57  LDKHETSLETVMRYYDQLCSVEPKFPFTESQLCLTFTWKDAFDKGSIF--GGSVKLALPS 116

Query: 158 LRFEVGMILFLYGAVLRQRAVEV---MSEDIVQSATFFREAAGVYQYLAKEILPSIQHCL 217
           L +E   +LF  GA+  Q A E      E +  ++ F++ A+G + ++   +L S+    
Sbjct: 117 LGYEKTCVLFNIGALASQIASEQNLDNDEALKAASKFYQLASGAFSHIKDTVLSSLNR-- 176

Query: 218 PSEKPPEVIPSTSVAMSLVCLAEAQAVTTMRAEEKGTISSLLAKLHYGIVDLLNESANHL 277
             +   ++ P T   +SL+ LA+AQ V  ++A       +++AK       L N++A++ 
Sbjct: 177 --DPTVDISPDTVGTLSLIMLAQAQEVFFLKATRDKMKDAVIAK-------LANQAADYY 236

Query: 278 RIISGECK---DISSNFLEFLSAFRALHELKCWKNIAKDLMSGGHAGVAIGVLRHALTDV 337
                +C+    +S      L+A   + +     + +         G  IG L+HA   V
Sbjct: 237 GDAFKQCQYKDTLSKEVFPILAAKHCIMQAHAEYHQSVLAKQQKKFGEEIGRLQHASDLV 296

Query: 338 KKEMPRENSWKLIFGTEIDIVAETLRKFERENEIVWHKKIPSRDELPTPKGSQIVKSIP 391
           K    R + +  +     D +   L   +++N+ ++H ++P   +L     + +VKS P
Sbjct: 297 KTVSSRYDEYVNVKDL-ADKINRALTAAKKDNDFIYHDRVPDLKDLDPVGKASLVKSTP 341

BLAST of Spg010056 vs. ExPASy Swiss-Prot
Match: Q9QZA2 (Programmed cell death 6-interacting protein OS=Rattus norvegicus OX=10116 GN=Pdcd6ip PE=1 SV=2)

HSP 1 Score: 57.8 bits (138), Expect = 3.4e-07
Identity = 67/304 (22.04%), Postives = 123/304 (40.46%), Query Frame = 0

Query: 98  LHKLQQYLPLLENFVSQVDLVDYKLKLVQWTSELKIRWSSALSSSSLFQLRGPKFFQIDN 157
           L K +  L  L  +  Q+  ++ K    +    L   W  A    SLF   G     + +
Sbjct: 58  LDKHEGALETLLRYYDQICSIEPKFPFSENQICLTFTWKDAFDKGSLF--GGSVKLALAS 117

Query: 158 LRFEVGMILFLYGAVLRQRAVEV---MSEDIVQSATFFREAAGVYQYLAKEILPSIQHCL 217
           L +E   +LF   A+  Q A E      E +  +A  ++ A+G + ++   +L +    L
Sbjct: 118 LGYEKSCVLFNCAALASQIAAEQNLDNDEGLKTAAKQYQFASGAFLHIKDTVLSA----L 177

Query: 218 PSEKPPEVIPSTSVAMSLVCLAEAQAVTTMRAEEKGTISSLLAKLHYGIVDLLNESANHL 277
             E   ++ P T   +SL+ LA+AQ V  ++A       +++AK       L N++A++ 
Sbjct: 178 SREPTVDISPDTVGTLSLIMLAQAQEVFFLKATRDKMKDAIIAK-------LANQAADYF 237

Query: 278 RIISGEC--KDISSNFLEFLSAFRALHELKCWKNIAKDLMSGGHA------GVAIGVLRH 337
                +C  KD    +  F   F  L   +C      +      A      G  I  L+H
Sbjct: 238 GDAFKQCQYKDALPKYFYFQEVFPTLAAKQCIMQANAEYHQSILAKQQKKFGEEIARLQH 297

Query: 338 ALTDVKKEMPRENSWKLIFGTEIDIVAETLRKFERENEIVWHKKIPSRDELPTPKGSQIV 391
           A   +K    R + +  +     D +   L   +++N+ ++H ++P   +L     + +V
Sbjct: 298 AAELIKNVASRYDEYVNVKDFS-DKINRALAAAKKDNDFIYHDRVPDLKDLDPIGKATLV 347

BLAST of Spg010056 vs. ExPASy Swiss-Prot
Match: Q12033 (pH-response regulator protein palA/RIM20 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=RIM20 PE=1 SV=1)

HSP 1 Score: 56.2 bits (134), Expect = 9.8e-07
Identity = 63/263 (23.95%), Postives = 121/263 (46.01%), Query Frame = 0

Query: 129 SELKIRWSSALSSSSLFQLRGPKFFQIDNLRFEVGMILFLYG---AVLRQRAVEVMSEDI 188
           ++++  W   LS  S    RG   +   +L++E   I++  G   ++L   +    +E +
Sbjct: 80  NQIEFTWFQTLSQKS----RGTSQY---SLQWEKLTIIYNIGCMYSLLALNSNNDAAESL 139

Query: 189 VQSATFFREAAGVYQYLAKEILPSIQHCLPSEKPPEVIPSTSVAMSLVCLAEAQAVTTMR 248
             S  +F+ AAG ++++       + H    E  P V  +T  A++ + LA+AQ     +
Sbjct: 140 KTSCLYFQNAAGCFKHV-------LDHQKNLETIPVVDDATLNALTSLMLAQAQECFWFK 199

Query: 249 AEEKGTISSLLAKLHYGIVDLLNESANHLRIISGECKDISSNFLEFLSAFRALHELKCWK 308
           A +     SL+AKL   IVD   E+ N  +      K I S+++  L A +A      + 
Sbjct: 200 AVQDKHKDSLIAKLSQQIVDFYCEAINDAQ----RGKLIRSDWINHLKAKKAYFSAVTYY 259

Query: 309 NIAKDLMSGGHAGVAIGVLRHALTDVKKE-MPRENSWKLIFGTEIDIVAETLRKFERENE 368
            IA         G  +  L+  L  + +  +  +  +K        +V  +L++ +R+NE
Sbjct: 260 RIALSFNEKKQFGNVVKALQMGLQFINESTLSSQAKFK-------TVVESSLKEAQRDNE 315

Query: 369 IVWHKKIPSRDELPTPKGSQIVK 388
            ++ +++PS  ELP+ K + +VK
Sbjct: 320 FIYLQEVPS--ELPSIKPALMVK 315

BLAST of Spg010056 vs. ExPASy Swiss-Prot
Match: Q8WUM4 (Programmed cell death 6-interacting protein OS=Homo sapiens OX=9606 GN=PDCD6IP PE=1 SV=1)

HSP 1 Score: 54.7 bits (130), Expect = 2.8e-06
Identity = 63/299 (21.07%), Postives = 124/299 (41.47%), Query Frame = 0

Query: 98  LHKLQQYLPLLENFVSQVDLVDYKLKLVQWTSELKIRWSSALSSSSLFQLRGPKFFQIDN 157
           L K +  L  L  +  Q+  ++ K    +    L   W  A    SLF   G     + +
Sbjct: 58  LDKHEGALETLLRYYDQICSIEPKFPFSENQICLTFTWKDAFDKGSLF--GGSVKLALAS 117

Query: 158 LRFEVGMILFLYGAVLRQRAVEV---MSEDIVQSATFFREAAGVYQYLAKEILPSIQHCL 217
           L +E   +LF   A+  Q A E      E +  +A  ++ A+G + ++ + +L +    L
Sbjct: 118 LGYEKSCVLFNCAALASQIAAEQNLDNDEGLKIAAKHYQFASGAFLHIKETVLSA----L 177

Query: 218 PSEKPPEVIPSTSVAMSLVCLAEAQAVTTMRAEEKGTISSLLAKLHYGIVDLLNESANHL 277
             E   ++ P T   +SL+ LA+AQ V  ++A       +++AK       L N++A++ 
Sbjct: 178 SREPTVDISPDTVGTLSLIMLAQAQEVFFLKATRDKMKDAIIAK-------LANQAADYF 237

Query: 278 RIISGECK---DISSNFLEFLSAFRALHELKCWKNIAKDLMSGGHAGVAIGVLRHALTDV 337
                +C+    +       L+A   + +     + +         G  I  L+HA   +
Sbjct: 238 GDAFKQCQYKDTLPKEVFPVLAAKHCIMQANAEYHQSILAKQQKKFGEEIARLQHAAELI 297

Query: 338 KKEMPRENSWKLIFGTEIDIVAETLRKFERENEIVWHKKIPSRDELPTPKGSQIVKSIP 391
           K    R + +  +     D +   L   +++N+ ++H ++P   +L     + +VKS P
Sbjct: 298 KTVASRYDEYVNVKDFS-DKINRALAAAKKDNDFIYHDRVPDLKDLDPIGKATLVKSTP 342

BLAST of Spg010056 vs. ExPASy Swiss-Prot
Match: Q8WZL4 (pH-response regulator protein RIM20 OS=Yarrowia lipolytica (strain CLIB 122 / E 150) OX=284591 GN=RIM20 PE=3 SV=2)

HSP 1 Score: 54.3 bits (129), Expect = 3.7e-06
Identity = 71/291 (24.40%), Postives = 126/291 (43.30%), Query Frame = 0

Query: 117 LVDYKLKLVQWTSE-------LKIRWSSALSSSSLFQLRGPKFFQIDNLRFEVGMILFLY 176
           L  Y  +L  WT++       L+  W   L+ ++      P   Q  +L FE   +L+  
Sbjct: 63  LTKYYAQLTYWTTKFPAGCDSLEFMWYGTLAYTA---NAAPVISQ--SLHFERCNLLYNL 122

Query: 177 GAVLRQRAVEVMSED---IVQSATFFREAAGVYQYLAKEILPSIQHCLPSEKPPEVIPST 236
           G++  Q  V    +D   +  S  +F+ AAG +Q L +  LP ++    S     +   T
Sbjct: 123 GSLYSQMGVNEGRQDADGLKMSFNYFQMAAGCFQILIENGLPDLE----SLNMRGLEYDT 182

Query: 237 SVAMSLVCLAEAQAVTTMRAEEKGTISSLLAKLHYGIVDLLNESANHLRIISGECKDISS 296
              +  + LA+AQ     R+   G+ +SL+AKL    V LL +SA      + +   + S
Sbjct: 183 ICCVRDLMLAQAQECFWQRSLLSGSRNSLVAKLAQQ-VSLLYDSA---LAWAQKSPQVRS 242

Query: 297 NFLEFLSAFRALHELKCWKNIAKDLMSGGHAGVAIGVLRHALTDVK----KEMPRENSWK 356
            ++  ++  R          +A+D + G   G  I  L+ AL  +K    K M R    +
Sbjct: 243 EWVHHMTCKRQHFLAVAHYRMARDALDGCRYGEEIARLKAALNAIKEGTGKGMLRRYLVE 302

Query: 357 LI---FGTEIDIVAETLRKFERENEIVWHKKIPSRDELPTPKGSQIVKSIP 391
            +       ID+V E L++ E++N +++ + +P    L     + +VK  P
Sbjct: 303 SVADDLNGIIDVVTEDLKRAEKDNNLIYLQSVPQTTALAPITPALMVKCTP 340

BLAST of Spg010056 vs. ExPASy TrEMBL
Match: A0A6J1FZA5 (uncharacterized protein LOC111449253 OS=Cucurbita moschata OX=3662 GN=LOC111449253 PE=4 SV=1)

HSP 1 Score: 637.1 bits (1642), Expect = 5.0e-179
Identity = 330/376 (87.77%), Postives = 351/376 (93.35%), Query Frame = 0

Query: 30  RFSDEFR-KPGRCVFEEKFIARDSGTLEQLKEMTSRRRVIEESINETSSITDAIAREMYG 89
           +FSD  + K   CVFE+KFIARDS TLEQLKEMTSRRRVIEESINETSSITDAIAREM G
Sbjct: 4   QFSDPAKLKTKMCVFEDKFIARDSSTLEQLKEMTSRRRVIEESINETSSITDAIAREMSG 63

Query: 90  GLSSHTQQVLHKLQQYLPLLENFVSQVDLVDYKLKLVQWTSELKIRWSSALSSSSLFQLR 149
           GLSSHTQQVLHKL QYLPLLENFVSQVDLV Y LK+VQWTS+LKIRWSSALSSSSLF LR
Sbjct: 64  GLSSHTQQVLHKLLQYLPLLENFVSQVDLVGYNLKIVQWTSDLKIRWSSALSSSSLFHLR 123

Query: 150 GPKFFQIDNLRFEVGMILFLYGAVLRQRAVEVMSEDIVQSATFFREAAGVYQYLAKEILP 209
           GPKFFQIDNLRFEV M LFLYGAVLRQRA+EV+SED VQSATFFREAAGVYQYLAK+ILP
Sbjct: 124 GPKFFQIDNLRFEVAMTLFLYGAVLRQRALEVISEDQVQSATFFREAAGVYQYLAKDILP 183

Query: 210 SIQHCLPSEKPPEVIPSTSVAMSLVCLAEAQAVTTMRAEEKGTISSLLAKLHYGIVDLLN 269
           SIQHC+PSE+PPEVIPSTSVAMSLVCLAEAQAVTTMRAEEKG+ISSLLAKLHYGIVDLLN
Sbjct: 184 SIQHCVPSERPPEVIPSTSVAMSLVCLAEAQAVTTMRAEEKGSISSLLAKLHYGIVDLLN 243

Query: 270 ESANHLRIISGECKDISSNFLEFLSAFRALHELKCWKNIAKDLMSGGHAGVAIGVLRHAL 329
           ESANHLRI+SGE    SS+FLEFLSAFRALHELK  KN+AK+LMSGG AGVAIG+LRHAL
Sbjct: 244 ESANHLRILSGE----SSSFLEFLSAFRALHELKSRKNLAKELMSGGQAGVAIGILRHAL 303

Query: 330 TDVKKEMPRENSWKLIFGTEIDIVAETLRKFERENEIVWHKKIPSRDELPTPKGSQIVKS 389
           TDVKK+MPRENSW+L+FGTEID VAETLRKFERENEIVWHKKIPSRDELPTPKGSQIV S
Sbjct: 304 TDVKKDMPRENSWRLVFGTEIDSVAETLRKFERENEIVWHKKIPSRDELPTPKGSQIVSS 363

Query: 390 IPYHPEKWERELAFKI 405
           IPY P+KWERELAFK+
Sbjct: 364 IPYCPKKWERELAFKM 375

BLAST of Spg010056 vs. ExPASy TrEMBL
Match: A0A1S3BFB8 (uncharacterized protein LOC103489257 OS=Cucumis melo OX=3656 GN=LOC103489257 PE=4 SV=1)

HSP 1 Score: 634.8 bits (1636), Expect = 2.5e-178
Identity = 323/376 (85.90%), Postives = 351/376 (93.35%), Query Frame = 0

Query: 30  RFSDEFR-KPGRCVFEEKFIARDSGTLEQLKEMTSRRRVIEESINETSSITDAIAREMYG 89
           +FSD  + K   CVFE+KFIARDSGTLEQLKEMTSRRRVIEESINETSSITDAIAREM G
Sbjct: 4   QFSDPSKLKTKMCVFEDKFIARDSGTLEQLKEMTSRRRVIEESINETSSITDAIAREMSG 63

Query: 90  GLSSHTQQVLHKLQQYLPLLENFVSQVDLVDYKLKLVQWTSELKIRWSSALSSSSLFQLR 149
           GLSSHTQQVLHKLQQYLPLLENF+SQVD++DY L++VQWTS+LKIRWSSALSSS LF LR
Sbjct: 64  GLSSHTQQVLHKLQQYLPLLENFISQVDMLDYNLRIVQWTSDLKIRWSSALSSSLLFHLR 123

Query: 150 GPKFFQIDNLRFEVGMILFLYGAVLRQRAVEVMSEDIVQSATFFREAAGVYQYLAKEILP 209
           GPKFFQIDNLRFEVGM+L LYGA LRQRA+EV+SED VQSATFFREAAGVYQYLA+EILP
Sbjct: 124 GPKFFQIDNLRFEVGMMLSLYGAFLRQRALEVISEDQVQSATFFREAAGVYQYLAEEILP 183

Query: 210 SIQHCLPSEKPPEVIPSTSVAMSLVCLAEAQAVTTMRAEEKGTISSLLAKLHYGIVDLLN 269
           +IQHCLPSE+PPEVIPSTS AMSL+CLAEAQAVT+MRAEEKGTI SLLAKLHYGIV+LLN
Sbjct: 184 TIQHCLPSERPPEVIPSTSAAMSLICLAEAQAVTSMRAEEKGTIPSLLAKLHYGIVELLN 243

Query: 270 ESANHLRIISGECKDISSNFLEFLSAFRALHELKCWKNIAKDLMSGGHAGVAIGVLRHAL 329
           ESANHL I SGE KD+SSNFLEFLSAFRALHEL   KN+AK+LMSGG +GVAIGVLR+AL
Sbjct: 244 ESANHLHISSGESKDMSSNFLEFLSAFRALHELMSRKNLAKELMSGGQSGVAIGVLRYAL 303

Query: 330 TDVKKEMPRENSWKLIFGTEIDIVAETLRKFERENEIVWHKKIPSRDELPTPKGSQIVKS 389
           TDVKKEMPRENSWKL+FG EIDIV ETLRKFERENEIVWHKKIPSRDELPTP G+QIVKS
Sbjct: 304 TDVKKEMPRENSWKLVFGIEIDIVTETLRKFERENEIVWHKKIPSRDELPTPVGTQIVKS 363

Query: 390 IPYHPEKWERELAFKI 405
           IPY+P+KWER+LAFKI
Sbjct: 364 IPYNPKKWERDLAFKI 379

BLAST of Spg010056 vs. ExPASy TrEMBL
Match: A0A6J1HUA9 (uncharacterized protein LOC111467531 OS=Cucurbita maxima OX=3661 GN=LOC111467531 PE=4 SV=1)

HSP 1 Score: 629.8 bits (1623), Expect = 7.9e-177
Identity = 327/376 (86.97%), Postives = 349/376 (92.82%), Query Frame = 0

Query: 30  RFSDEFR-KPGRCVFEEKFIARDSGTLEQLKEMTSRRRVIEESINETSSITDAIAREMYG 89
           +FSD  + K   CVFE+KFIARDS TLEQLKEMTSRR+VIEESINETS ITDAIAREM G
Sbjct: 4   QFSDPAKLKTKMCVFEDKFIARDSSTLEQLKEMTSRRKVIEESINETSFITDAIAREMSG 63

Query: 90  GLSSHTQQVLHKLQQYLPLLENFVSQVDLVDYKLKLVQWTSELKIRWSSALSSSSLFQLR 149
           GLSSHTQQVLHKL QYLPLLENFVSQVDLV Y LK+VQWTS+LKIRWSSALSSSSLF LR
Sbjct: 64  GLSSHTQQVLHKLLQYLPLLENFVSQVDLVGYNLKIVQWTSDLKIRWSSALSSSSLFHLR 123

Query: 150 GPKFFQIDNLRFEVGMILFLYGAVLRQRAVEVMSEDIVQSATFFREAAGVYQYLAKEILP 209
           GPKFFQIDNLRFEV M LFLYGAVLR+RA+EV+SED VQSATFFREAAGVYQYLAK+ILP
Sbjct: 124 GPKFFQIDNLRFEVAMTLFLYGAVLRKRALEVISEDKVQSATFFREAAGVYQYLAKDILP 183

Query: 210 SIQHCLPSEKPPEVIPSTSVAMSLVCLAEAQAVTTMRAEEKGTISSLLAKLHYGIVDLLN 269
           SIQHC+PSE+PPEVIPSTSVAMSLVCLAEAQAVTTMRAEEKG+ISSLLAKLHYGIVDLLN
Sbjct: 184 SIQHCVPSERPPEVIPSTSVAMSLVCLAEAQAVTTMRAEEKGSISSLLAKLHYGIVDLLN 243

Query: 270 ESANHLRIISGECKDISSNFLEFLSAFRALHELKCWKNIAKDLMSGGHAGVAIGVLRHAL 329
           ESANHLRI SGE    SS+FLEFLSAFRALHELK  KN+AK+LMSGG  G+AIG+LRHAL
Sbjct: 244 ESANHLRIFSGE----SSSFLEFLSAFRALHELKSRKNLAKELMSGGQVGLAIGILRHAL 303

Query: 330 TDVKKEMPRENSWKLIFGTEIDIVAETLRKFERENEIVWHKKIPSRDELPTPKGSQIVKS 389
           TDVKKEMPRENSWKL+FGTEIDIVAETLRKFERENEIVWHKKIPSRDELPT KGS+IVKS
Sbjct: 304 TDVKKEMPRENSWKLVFGTEIDIVAETLRKFERENEIVWHKKIPSRDELPTLKGSRIVKS 363

Query: 390 IPYHPEKWERELAFKI 405
           IPY P+KWERELAFK+
Sbjct: 364 IPYCPKKWERELAFKM 375

BLAST of Spg010056 vs. ExPASy TrEMBL
Match: A0A0A0KR44 (BRO1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G608410 PE=4 SV=1)

HSP 1 Score: 626.7 bits (1615), Expect = 6.7e-176
Identity = 320/376 (85.11%), Postives = 348/376 (92.55%), Query Frame = 0

Query: 30  RFSDEFR-KPGRCVFEEKFIARDSGTLEQLKEMTSRRRVIEESINETSSITDAIAREMYG 89
           +FSD  + K   CVFE+KFIARDSGTLEQLKE+TSRRRVIEESINETSSITDAIAREM G
Sbjct: 4   QFSDPAKLKTKMCVFEDKFIARDSGTLEQLKEITSRRRVIEESINETSSITDAIAREMSG 63

Query: 90  GLSSHTQQVLHKLQQYLPLLENFVSQVDLVDYKLKLVQWTSELKIRWSSALSSSSLFQLR 149
           GLSSHTQQVLHKLQQYLPLLENFVSQVD++DY L++VQWTS+LKIRWSSALSSSSLF LR
Sbjct: 64  GLSSHTQQVLHKLQQYLPLLENFVSQVDMIDYNLRIVQWTSDLKIRWSSALSSSSLFHLR 123

Query: 150 GPKFFQIDNLRFEVGMILFLYGAVLRQRAVEVMSEDIVQSATFFREAAGVYQYLAKEILP 209
           GPKFFQIDNLRFEVGM L LYGA LRQRA+E++SED VQSATFFREA+GVYQYLA+EILP
Sbjct: 124 GPKFFQIDNLRFEVGMTLSLYGAFLRQRALEIISEDQVQSATFFREASGVYQYLAEEILP 183

Query: 210 SIQHCLPSEKPPEVIPSTSVAMSLVCLAEAQAVTTMRAEEKGTISSLLAKLHYGIVDLLN 269
           +IQHCLPSE+PPEVIPSTS AMSLVCLAEAQAVT+MRAEEKGTI SLLAKLHYGIV+LLN
Sbjct: 184 TIQHCLPSERPPEVIPSTSAAMSLVCLAEAQAVTSMRAEEKGTIPSLLAKLHYGIVELLN 243

Query: 270 ESANHLRIISGECKDISSNFLEFLSAFRALHELKCWKNIAKDLMSGGHAGVAIGVLRHAL 329
           ESAN+L I  GECKD+SSNFLEFLSAFRALHEL   KN+AK+LMS G  GVAIGVLR+AL
Sbjct: 244 ESANYLHISFGECKDMSSNFLEFLSAFRALHELMSRKNLAKELMSSGQVGVAIGVLRYAL 303

Query: 330 TDVKKEMPRENSWKLIFGTEIDIVAETLRKFERENEIVWHKKIPSRDELPTPKGSQIVKS 389
           TDVKKEMPRENSWKL+FG EIDIVAETLRKFERENEIVWHKKI  RDELPTP G++IVKS
Sbjct: 304 TDVKKEMPRENSWKLVFGIEIDIVAETLRKFERENEIVWHKKIALRDELPTPIGTRIVKS 363

Query: 390 IPYHPEKWERELAFKI 405
           IPY+P+KWERELAFKI
Sbjct: 364 IPYNPKKWERELAFKI 379

BLAST of Spg010056 vs. ExPASy TrEMBL
Match: A0A5A7SYL1 (BRO1 domain-containing protein BROX OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G00750 PE=4 SV=1)

HSP 1 Score: 591.7 bits (1524), Expect = 2.4e-165
Identity = 300/340 (88.24%), Postives = 322/340 (94.71%), Query Frame = 0

Query: 41  CVFEEKFIARDSGTLEQLKEMTSRRRVIEESINETSSITDAIAREMYGGLSSHTQQVLHK 100
           CVFE+KFIARDSGTLEQLKEMTSRRRVIEESINETSSITDAIAREM GGLSSHTQQVLHK
Sbjct: 2   CVFEDKFIARDSGTLEQLKEMTSRRRVIEESINETSSITDAIAREMSGGLSSHTQQVLHK 61

Query: 101 LQQYLPLLENFVSQVDLVDYKLKLVQWTSELKIRWSSALSSSSLFQLRGPKFFQIDNLRF 160
           LQQYLPLLENF+SQVD++DY L++VQWTS+LKIRWSSALSSS LF LRGPKFFQIDNLRF
Sbjct: 62  LQQYLPLLENFISQVDMLDYNLRIVQWTSDLKIRWSSALSSSLLFHLRGPKFFQIDNLRF 121

Query: 161 EVGMILFLYGAVLRQRAVEVMSEDIVQSATFFREAAGVYQYLAKEILPSIQHCLPSEKPP 220
           EVGM+L LYGA LRQRA+EV+SED VQSATFFREAAGVYQYLA+EILP+IQHCLPSE+PP
Sbjct: 122 EVGMMLSLYGAFLRQRALEVISEDQVQSATFFREAAGVYQYLAEEILPTIQHCLPSERPP 181

Query: 221 EVIPSTSVAMSLVCLAEAQAVTTMRAEEKGTISSLLAKLHYGIVDLLNESANHLRIISGE 280
           EVIPSTS AMSL+CLAEAQAVT+MRAEEKGTI SLLAKLHYGIV+LLNESANHL I SGE
Sbjct: 182 EVIPSTSAAMSLICLAEAQAVTSMRAEEKGTIPSLLAKLHYGIVELLNESANHLHISSGE 241

Query: 281 CKDISSNFLEFLSAFRALHELKCWKNIAKDLMSGGHAGVAIGVLRHALTDVKKEMPRENS 340
            KD+SSNFLEFLSAFRALHEL   KN+AK+LMSGG +GVAIGVLR+ALTDVKKEMPRENS
Sbjct: 242 SKDMSSNFLEFLSAFRALHELMSRKNLAKELMSGGQSGVAIGVLRYALTDVKKEMPRENS 301

Query: 341 WKLIFGTEIDIVAETLRKFERENEIVWHKKIPSRDELPTP 381
           WKL+FG EIDIV ETLRKFERENEIVWHKKIPSRDELPTP
Sbjct: 302 WKLVFGIEIDIVTETLRKFERENEIVWHKKIPSRDELPTP 341

BLAST of Spg010056 vs. TAIR 10
Match: AT1G13310.1 (Endosomal targeting BRO1-like domain-containing protein )

HSP 1 Score: 309.7 bits (792), Expect = 3.5e-84
Identity = 175/369 (47.43%), Postives = 242/369 (65.58%), Query Frame = 0

Query: 37  KPGRCVFEEKFIARDSGTLEQLKEMTSRRRVIEESINETSSITDAIAREMYGGLSSHTQQ 96
           K    ++E+   A    TL ++KE+TS+R++IEES+N+TS + D+  REM  GL+S  +Q
Sbjct: 12  KTKEILYEKTLQAAGPITLGEVKELTSKRKIIEESVNKTSKVIDSTTREMTRGLTSACEQ 71

Query: 97  VLHKLQQYLPLLENFVSQVDLVDYKLKLVQWTSELKIRWSSALSSSSLFQLRGPKFFQID 156
            LHKL +YLPLL N V   D    K+K V   S LKIRWSS L S +L Q   PKFFQ+D
Sbjct: 72  DLHKLGEYLPLLFNLVHYTD----KIKRV---SNLKIRWSSGLISQTLIQRTCPKFFQVD 131

Query: 157 NLRFEVGMILFLYGAVLRQRAVEVMSEDIVQSATFFREAAGVYQYLAKEILPSIQHCLPS 216
           N+ FE GM+LF+Y   LR+RA+E++S D  ++   +REA+GV+ +L+ EILP +Q CLP 
Sbjct: 132 NIMFEFGMVLFIYAVKLRERAMELVSTDSKKAVAVYREASGVFHHLSHEILPLLQTCLPP 191

Query: 217 EKPPEVIPSTSVAMSLVCLAEAQAVTTMRAEEKGTISSLLAKLHYGIVDLLNESANHLRI 276
            K PE+ P    A+SL+CLAE QAVTT +AEE G  +SLL+KLH+GI   L+E+   L  
Sbjct: 192 GKLPELTPPLCSALSLLCLAEGQAVTTEKAEESGKSASLLSKLHFGIFQFLSEAYALLSS 251

Query: 277 -ISGECKDISSNFLEFLSAFRALHELKCWKNIAKDLMSGGHAGVAIGVLRHALTDVKKEM 336
            ++GE KD+S+ FLE+++   ALHELK  K +A+ L S    G A+GVLR AL   KK  
Sbjct: 252 RLTGEYKDLSTRFLEYVTTMGALHELKSQKYLAELLESEDRVGDAVGVLRRALAAAKKST 311

Query: 337 P-RENSWKLIFGTEIDIVAETLRKFERENEIVWHKKIPSRDELPTPKGSQIVKSIPYHPE 396
           P +++ W  IF  E + VA+ + K+E+ N+ +  +KIP   E+P PKG +IV  IPY P 
Sbjct: 312 PSKDDKWIAIFKKEREDVAKNMAKYEKLNDSMMLQKIPIDREIPFPKGEKIVNLIPYTPT 371

Query: 397 KWERELAFK 404
           +  REL FK
Sbjct: 372 RVVRELRFK 373