Spg009980 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg009980
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	calumenin-B
Location	scaffold7: 7428392 .. 7430725 (-)
RNA-Seq Expression	Spg009980
Synteny	Spg009980

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTGAAAATCTCCATTTTCATCTGCACATCAATCTTTTCCCTTCTCCTATTCTTCACTTCTCACACCTCAATTCGACACCATTCCCACTCCAATGCCGCCGACTCCCACCCCGAGTGGGAAGAGTTCATCAACGACGAGGATAGGTTCAATGTCACCGAAAGATTGGTGTCCCTTTTCCCCAAAATCGACGTCGATCCTCATGATGGGTTCGTGTCTGCGGAGGAATTGACTCGGTGGAACTTGCAGCAGGCCTTGAACGAGGCTCTGCATCGGACTGAAAGGGAATTTCAATCTCACGATGAGAATCGTGATGGGTTCGTTTCCTTTGCTGAGTACGAGCCGCCCAGTTGTTCTTGATCGTTCTAGCTTCTTTTTCAATTGGATTTTACTGTTGTCTGGTTCTTGATTTTGGATCCTCTGCAAATTAATGTGCTGCTGTTTGGTGAAGTTGGATTTTAGTATCTTTTGTCGGTTACTTTGGCAATTTAGTACTTTATGGTATACTATTTTCTATGATATGTTTGTTTGTTTTCCAATGGCATTTATGATTTACTTTACCAGTTTTTGTAGTAGAAAGTTACCTTTTGTACAAGAGAAATGAAGATATTATTTGTATATATGCTTTTGTGAGATATTTTATACATATATATTTGGCTCATATAGTTTATGATGTTTGGAATTGAGACATTTGAGTTTGTATAGAAATGTAGATAGACATAAGTTAGACCTGAGTTTATGTAATTTATGAGTTTTTTCTTTTCCTTAGAGAAAGTGTAACATGTGTTTGATAGGTAGATCTATGTGATTTGTTTAGAAAGTGAACCTGTGTTTGATTTGTGAGTTTATAACTTGTGAGTTCGTGACGGATAAAAGTTTACCATATAATCTTAAAGGTAGGTTTCATAAGCTTAGATTTTTTAATCTAGATTTATGTTGACCCTAGGAATAACAATTCAGTAGTTTGGAATAAGCAAATCCATACACTAAATATAGGCTTCGGTTCTTAAACCTTGTTAGACAAGTCAAACATCCCTAAATGGCTTCTTCTTCTGCTTCTCATAGAAGTTTTTTGAAGAATCCAGCGGTGTTGTTTATGTTACATATTCTGATGTTTAATTTCCAATCTCTCATCAGGCAATGGTTCTTTCAGCTATGATCTTGGCTGGTGGAATGAGGAGCACTTCAATGCATCAGGTGCAAATGGGGATGGGGTTTTGGACATAACTGAATTCGATGAGTAATCTTCAAAAACTTCACCGTGTCTCTTTTATCCGTATTCTGTTTGAAAATGCCAAGAAAAAGAGTTCATATTTCATCGGTTTCTCTTACTCGATACTTAACGAGCCATGATCTACTTGTTTTCAGTTTCTTGCATCCTGCTGACAGCAAGAGCCCAAAGCTACTTCTATGGCTGTGTGCAGATGTGGTAAGGTAGATTCCTTTGGTTCATAGTCAAATTTGGCCTTCTGCAGTTTGTATTTGTACTTGACTGTTGCTGTAAGTCCACAAAGAAGCTTATTACCAAGATGGTTCAACATAATGAACAAAAACCAGCAAGGAAGGCCTTAGATTTACAAGATTCTAATACTTGCCCCAATCTTCTCAGTTTCAAAGTCTTATGTTGTGTTCGAAGTTAGAATTTGATTCAGTCTCACACGATCATTCCACTGACCGCTTGCTTTATTATTTTACCGATCAGGGAAAGAGATGCAGACAATGATGGAAAGCTCAACCTCAGTGAGTTTTTCCCTGGAATTTTTTACTTAATAAGAAGTGGCGAAGAAGATTACGGTTCTTCAGATCAGGATGATGAACAAATGGAGGCACTGGCGAAAAAGGTGTTCTCTGTGCTTGACAAAGACTATGGTGGATATGTTGATATGCCTTGAATCTTTTATGAAGCGTCCAGAATGACCAAGTACAAGGGTTCGGGGGCAACACCCCCTAAACTAAATTATGTATAGAGATTAGATATGTTGTTTATGCTTTAGGCTTTTATTTTGGACCTATTCTCTTCGGGTTAAGAGTGGTGCTATATATAAACTCTCTTAACCTAATATGCCCTAATCTCTGTAATCTGCATATGTATTTTCCTCACTAATAAGATCGTTCTCTCCCTCTGCCCGTGGATGTAGCTAACACACCGTTAGTGAACCACGTAAATTCTGTGTTGCTTATTCGTCCTTTCATTCTTGTTCTGTTGATTTTAGTCTACTGTCATAACAGGGTAAAATATTGTTTCTAATTCCTATATATACCTTTATGCTGTAAATGATTCATTCTCTAAAACAGTATATTGTTCTGCTTCTTTACAGGTACCTTTCAGCAACA

mRNA sequence

ATGGTGAAAATCTCCATTTTCATCTGCACATCAATCTTTTCCCTTCTCCTATTCTTCACTTCTCACACCTCAATTCGACACCATTCCCACTCCAATGCCGCCGACTCCCACCCCGAGTGGGAAGAGTTCATCAACGACGAGGATAGGTTCAATGTCACCGAAAGATTGGTGTCCCTTTTCCCCAAAATCGACGTCGATCCTCATGATGGGTTCGTGTCTGCGGAGGAATTGACTCGGTGGAACTTGCAGCAGGCCTTGAACGAGGCTCTGCATCGGACTGAAAGGGAATTTCAATCTCACGATGAGAATCGTGATGGGTTCGTTTCCTTTGCTGAGTACGAGCCGCCCAGTTGCAATGGTTCTTTCAGCTATGATCTTGGCTGGTGGAATGAGGAGCACTTCAATGCATCAGGTGCAAATGGGGATGGGGTTTTGGACATAACTGAATTCGATGATTTCTTGCATCCTGCTGACAGCAAGAGCCCAAAGCTACTTCTATGGCTGTGTGCAGATGTGGTAAGGGAAAGAGATGCAGACAATGATGGAAAGCTCAACCTCAGTGAGTTTTTCCCTGGAATTTTTTACTTAATAAGAAGTGGCGAAGAAGATTACGGTTCTTCAGATCAGGATGATGAACAAATGGAGGCACTGGCGAAAAAGGTGTTCTCTGTGCTTGACAAAGACTATGGTGGATATGTTGATATGCCTTGA

Coding sequence (CDS)

ATGGTGAAAATCTCCATTTTCATCTGCACATCAATCTTTTCCCTTCTCCTATTCTTCACTTCTCACACCTCAATTCGACACCATTCCCACTCCAATGCCGCCGACTCCCACCCCGAGTGGGAAGAGTTCATCAACGACGAGGATAGGTTCAATGTCACCGAAAGATTGGTGTCCCTTTTCCCCAAAATCGACGTCGATCCTCATGATGGGTTCGTGTCTGCGGAGGAATTGACTCGGTGGAACTTGCAGCAGGCCTTGAACGAGGCTCTGCATCGGACTGAAAGGGAATTTCAATCTCACGATGAGAATCGTGATGGGTTCGTTTCCTTTGCTGAGTACGAGCCGCCCAGTTGCAATGGTTCTTTCAGCTATGATCTTGGCTGGTGGAATGAGGAGCACTTCAATGCATCAGGTGCAAATGGGGATGGGGTTTTGGACATAACTGAATTCGATGATTTCTTGCATCCTGCTGACAGCAAGAGCCCAAAGCTACTTCTATGGCTGTGTGCAGATGTGGTAAGGGAAAGAGATGCAGACAATGATGGAAAGCTCAACCTCAGTGAGTTTTTCCCTGGAATTTTTTACTTAATAAGAAGTGGCGAAGAAGATTACGGTTCTTCAGATCAGGATGATGAACAAATGGAGGCACTGGCGAAAAAGGTGTTCTCTGTGCTTGACAAAGACTATGGTGGATATGTTGATATGCCTTGA

Protein sequence

MVKISIFICTSIFSLLLFFTSHTSIRHHSHSNAADSHPEWEEFINDEDRFNVTERLVSLFPKIDVDPHDGFVSAEELTRWNLQQALNEALHRTEREFQSHDENRDGFVSFAEYEPPSCNGSFSYDLGWWNEEHFNASGANGDGVLDITEFDDFLHPADSKSPKLLLWLCADVVRERDADNDGKLNLSEFFPGIFYLIRSGEEDYGSSDQDDEQMEALAKKVFSVLDKDYGGYVDMP

Homology

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BLAST of Spg009980 vs. NCBI nr
Match: XP_038892509.1 (calumenin-B-like [Benincasa hispida] >XP_038892510.1 calumenin-B-like [Benincasa hispida])

HSP 1 Score: 341.3 bits (874), Expect = 6.8e-90
Identity = 183/289 (63.32%), Postives = 202/289 (69.90%), Query Frame = 0


Query: 1   MVKISIFICTSIFSLLLFFTSHT-----SIRH---------------------------- 60
           M K++I I  SIFSLLL   SHT      +RH                            
Sbjct: 1   MGKLAILIYISIFSLLLILISHTPTRRLRLRHRRLRLRSNFTFNPLLHRQQHDHYIPFDP 60

Query: 61  ----------------HS--HSNAADSHPEWEEFINDEDRFNVTERLVSLFPKIDVDPHD 120
                           HS  HS AADSHPEWEEFINDEDRFNVTERL  LFPKIDVDP D
Sbjct: 61  LIADIQLRREDDEWQKHSLQHSAAADSHPEWEEFINDEDRFNVTERLFWLFPKIDVDPSD 120

Query: 121 GFVSAEELTRWNLQQALNEALHRTEREFQSHDENRDGFVSFAEYEPPSC-----NGSFSY 180
           GFVSAEELTRWNLQQA+NEALHRTE+EFQSHD+NRDGFVSFAEYEPP+      N SF Y
Sbjct: 121 GFVSAEELTRWNLQQAMNEALHRTEKEFQSHDDNRDGFVSFAEYEPPTWVLAAGNDSFGY 180

Query: 181 DLGWWNEEHFNASGANGDGVLDITEFDDFLHPADSKSPKLLLWLCADVVRERDADNDGKL 234
           D+GWWNEEHFNAS A+GDGVLDI EF+DFLHPADSKSPKLLLWLCADVVRERD D DGKL
Sbjct: 181 DIGWWNEEHFNASDADGDGVLDINEFNDFLHPADSKSPKLLLWLCADVVRERDNDKDGKL 240

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BLAST of Spg009980 vs. NCBI nr
Match: XP_008445899.1 (PREDICTED: calumenin-B [Cucumis melo] >KAA0034085.1 calumenin-B [Cucumis melo var. makuwa])

HSP 1 Score: 339.7 bits (870), Expect = 2.0e-89
Identity = 163/206 (79.13%), Postives = 177/206 (85.92%), Query Frame = 0


Query: 33  AADSHPEWEEFINDEDRFNVTERLVSLFPKIDVDPHDGFVSAEELTRWNLQQALNEALHR 92
           A DSHPEWEEFINDEDRFNVTERL  +FPKIDVDP DGFVSAEELTRWNLQQA+NEALHR
Sbjct: 82  AHDSHPEWEEFINDEDRFNVTERLFFIFPKIDVDPSDGFVSAEELTRWNLQQAMNEALHR 141

Query: 93  TEREFQSHDENRDGFVSFAEYEPPSC-----NGSFSYDLGWWNEEHFNASGANGDGVLDI 152
           TEREFQSHDENRDGFVSFAEYEPPS      N SF YD+GWWNEEHFNAS A+GDGVLD+
Sbjct: 142 TEREFQSHDENRDGFVSFAEYEPPSWVLAAGNSSFGYDIGWWNEEHFNASDADGDGVLDL 201

Query: 153 TEFDDFLHPADSKSPKLLLWLCADVVRERDADNDGKLNLSEFFPGIFYLIRSGEEDYGSS 212
            EF+DFLHPADSKSPKLLLWLCADVVRERD D DGKLN SEFFP I  LIR  +EDY SS
Sbjct: 202 NEFNDFLHPADSKSPKLLLWLCADVVRERDNDKDGKLNFSEFFPKILDLIRRVDEDYNSS 261

Query: 213 DQDDEQMEALAKKVFSVLDKDYGGYV 234
           + +DE+ EALA+K+F  LDKD  G++
Sbjct: 262 NWEDEEPEALARKMFLELDKDSDGFL 287

[Close]

BLAST of Spg009980 vs. NCBI nr
Match: XP_011655504.1 (calumenin-B [Cucumis sativus] >XP_031741815.1 calumenin-B [Cucumis sativus] >KGN51567.1 hypothetical protein Csa_009120 [Cucumis sativus])

HSP 1 Score: 337.0 bits (863), Expect = 1.3e-88
Identity = 161/206 (78.16%), Postives = 177/206 (85.92%), Query Frame = 0


Query: 33  AADSHPEWEEFINDEDRFNVTERLVSLFPKIDVDPHDGFVSAEELTRWNLQQALNEALHR 92
           A DSHPEWEEFINDEDRFNVTERL  +FPKIDVDP DGFVSAEELTRWNLQQA+NEAL+R
Sbjct: 82  AHDSHPEWEEFINDEDRFNVTERLFWIFPKIDVDPSDGFVSAEELTRWNLQQAMNEALYR 141

Query: 93  TEREFQSHDENRDGFVSFAEYEPPSC-----NGSFSYDLGWWNEEHFNASGANGDGVLDI 152
           TEREFQSHD+NRDGFVSFAEYEPPS      NGSF YD+GWWNEEHFNAS A+GDGVLD+
Sbjct: 142 TEREFQSHDDNRDGFVSFAEYEPPSWVLSAGNGSFGYDIGWWNEEHFNASDADGDGVLDL 201

Query: 153 TEFDDFLHPADSKSPKLLLWLCADVVRERDADNDGKLNLSEFFPGIFYLIRSGEEDYGSS 212
            EF+DFLHPADSKS KLLLWLCADVVRERD D DGKLN SEFFP +  L+R  +EDY SS
Sbjct: 202 NEFNDFLHPADSKSTKLLLWLCADVVRERDNDKDGKLNFSEFFPKVLDLVRRVDEDYNSS 261

Query: 213 DQDDEQMEALAKKVFSVLDKDYGGYV 234
           + +DE+ EALAKK+F  LDKD  GY+
Sbjct: 262 NWEDEEPEALAKKMFLELDKDSDGYL 287

[Close]

BLAST of Spg009980 vs. NCBI nr
Match: KAG6601072.1 (45 kDa calcium-binding protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 323.6 bits (828), Expect = 1.5e-84
Identity = 154/211 (72.99%), Postives = 174/211 (82.46%), Query Frame = 0


Query: 29  SHSNAADSHPEWEEFINDEDRFNVTERLVSLFPKIDVDPHDGFVSAEELTRWNLQQALNE 88
           S S+AADSHPEWEEFINDEDRFNVTERL SLFP IDV+P DGF+S EELTRWN QQA+N 
Sbjct: 84  SQSDAADSHPEWEEFINDEDRFNVTERLSSLFPNIDVNPTDGFISVEELTRWNWQQAMNA 143

Query: 89  ALHRTEREFQSHDENRDGFVSFAEYEPPSC------NGSFSYDLGWWNEEHFNASGANGD 148
           ALHRTEREF++HDE+RDGFVSFAEYEPPS       N S  YD+GWWNEEHFNAS A+G+
Sbjct: 144 ALHRTEREFETHDEDRDGFVSFAEYEPPSWVMAAGNNSSSGYDIGWWNEEHFNASDADGN 203

Query: 149 GVLDITEFDDFLHPADSKSPKLLLWLCADVVRERDADNDGKLNLSEFFPGIFYLIRSGEE 208
           G LD++EF+DFLHPADS++PKLLLWLC DVVRERD   DGKLN SEF P IF L+R  +E
Sbjct: 204 GALDMSEFNDFLHPADSRNPKLLLWLCRDVVRERDNSKDGKLNFSEFSPAIFDLVRRVDE 263

Query: 209 DYGSSDQDDEQMEALAKKVFSVLDKDYGGYV 234
           DYGS   DD+QMEA AKK+F  LDKD  GY+
Sbjct: 264 DYGSLSSDDDQMEARAKKMFLELDKDEDGYL 294

[Close]

BLAST of Spg009980 vs. NCBI nr
Match: KAG7031877.1 (45 kDa calcium-binding protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 321.6 bits (823), Expect = 5.6e-84
Identity = 153/211 (72.51%), Postives = 174/211 (82.46%), Query Frame = 0


Query: 29  SHSNAADSHPEWEEFINDEDRFNVTERLVSLFPKIDVDPHDGFVSAEELTRWNLQQALNE 88
           S S+AADSHPEWEEFINDEDRFNVTERL SLFP IDV+P DGF+S EELTRWN QQA+N 
Sbjct: 84  SQSDAADSHPEWEEFINDEDRFNVTERLSSLFPNIDVNPTDGFISVEELTRWNWQQAMNA 143

Query: 89  ALHRTEREFQSHDENRDGFVSFAEYEPPSC------NGSFSYDLGWWNEEHFNASGANGD 148
           ALHRTEREF++HDE+RDGFVSFAEYEPPS       N S  YD+GWWNEEHFNAS A+G+
Sbjct: 144 ALHRTEREFETHDEDRDGFVSFAEYEPPSWVMAAGNNSSSGYDIGWWNEEHFNASDADGN 203

Query: 149 GVLDITEFDDFLHPADSKSPKLLLWLCADVVRERDADNDGKLNLSEFFPGIFYLIRSGEE 208
           G LD++EF+DFLHPADS++PKLLLWLC DVVRERD   DGKL+ SEF P IF L+R  +E
Sbjct: 204 GALDMSEFNDFLHPADSRNPKLLLWLCRDVVRERDNSKDGKLSFSEFSPAIFDLVRRVDE 263

Query: 209 DYGSSDQDDEQMEALAKKVFSVLDKDYGGYV 234
           DYGS   DD+QMEA AKK+F  LDKD  GY+
Sbjct: 264 DYGSLSSDDDQMEARAKKMFLELDKDEDGYL 294

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BLAST of Spg009980 vs. ExPASy Swiss-Prot
Match: Q7ZUC2 (45 kDa calcium-binding protein OS=Danio rerio OX=7955 GN=sdf4 PE=2 SV=1)

HSP 1 Score: 78.2 bits (191), Expect = 1.4e-13
Identity = 62/222 (27.93%), Postives = 101/222 (45.50%), Query Frame = 0


Query: 39  EWEEFINDEDRFNVTERLVSLFPKIDVDPHDGFVSAEELTRWNLQQA---LNEALHRTER 98
           E EEF  D +     ++L+ +F K+D++  D  VSA+E+ RW +++      EA+   + 
Sbjct: 80  EMEEFEEDSEPRRNRKKLIEIFTKVDIN-KDRSVSAKEMQRWIMEKTEEHFQEAVRENKL 139

Query: 99  EFQSHDENRDGFVSFAEY----------------EPPSCNGSFSYD------LGWWNEEH 158
            F++ D + DG V++ EY                E    N     D      L    +  
Sbjct: 140 SFRAVDPDGDGLVTWDEYRVKFLASKGLNEKEVAEKIKNNEELKVDEETQEVLESLKDRW 199

Query: 159 FNASGANGDGVLDITEFDDFLHPADSKSPKLLLWLCADVVRERDADNDGKLNLSEFFPGI 218
           F A     D +L+  EF  FLHP  S+   +L ++  ++VR+ D D DGKL L+EF    
Sbjct: 200 FQADNPPADQLLNEEEFLSFLHPEHSRG--MLRYMVKEIVRDLDQDGDGKLTLAEFIS-- 259

Query: 219 FYLIRSGEEDYGSSDQDDEQMEALAKKVFSVLDKDYGGYVDM 236
             L     E+  + D DD+ +    K+   V+D ++   V M
Sbjct: 260 --LPMGTVENQQAQDIDDDWVRERKKEFEEVIDANHDTIVTM 294

[Close]

BLAST of Spg009980 vs. ExPASy Swiss-Prot
Match: Q5ZKE5 (45 kDa calcium-binding protein OS=Gallus gallus OX=9031 GN=SDF4 PE=2 SV=2)

HSP 1 Score: 77.0 bits (188), Expect = 3.1e-13
Identity = 62/222 (27.93%), Postives = 103/222 (46.40%), Query Frame = 0


Query: 39  EWEEFINDEDRFNVTERLVSLFPKIDVDPHDGFVSAEELTRWNLQQA---LNEALHRTER 98
           E EEF  D +     ++L+ +F K+D+D +D  +SA+E+ RW +++      EA+   + 
Sbjct: 80  EMEEFEEDSEPRKNRKKLMVIFSKVDID-NDKKISAKEMQRWIMEKTDEHFQEAVEENKM 139

Query: 99  EFQSHDENRDGFVSFAEY----------------EPPSCNGSFSYD------LGWWNEEH 158
            F++ D + DG VS+ EY                E    N     D      L    +  
Sbjct: 140 HFRAVDPDGDGHVSWDEYKIKFLASKGLNEKEIAEKIKNNEELKIDEETQEVLDNLKDRW 199

Query: 159 FNASGANGDGVLDITEFDDFLHPADSKSPKLLLWLCADVVRERDADNDGKLNLSEFFPGI 218
           + A     D +L+  EF  FLHP  S+   +L ++  +++R+ D D D KL LSEF    
Sbjct: 200 YQADNPPPDMLLNEEEFLSFLHPEHSRG--MLKFMVKEIIRDLDQDGDKKLTLSEFIS-- 259

Query: 219 FYLIRSGEEDYGSSDQDDEQMEALAKKVFSVLDKDYGGYVDM 236
             L     E+  + D DD+ ++   K+   V+D ++ G V M
Sbjct: 260 --LPVGTVENQQAQDIDDDWVKDRRKEFEDVIDANHDGIVTM 294

[Close]

BLAST of Spg009980 vs. ExPASy Swiss-Prot
Match: Q66JA6 (45 kDa calcium-binding protein OS=Xenopus laevis OX=8355 GN=sdf4 PE=2 SV=1)

HSP 1 Score: 73.2 bits (178), Expect = 4.5e-12
Identity = 62/222 (27.93%), Postives = 99/222 (44.59%), Query Frame = 0


Query: 39  EWEEFINDEDRFNVTERLVSLFPKIDVDPHDGFVSAEELTRWNLQQA---LNEALHRTER 98
           E EEF  D +      +L ++F K+D +  D  +SA E+ RW +++      EA++  + 
Sbjct: 84  ELEEFDEDSEPRRNRRKLAAIFAKVDRN-EDKQISASEMQRWIMEKTEEHFQEAVNENKL 143

Query: 99  EFQSHDENRDGFVSFAEY----------------EPPSCNGSFSYD------LGWWNEEH 158
            F++ D + DG VS+ EY                E    N     D      L    +  
Sbjct: 144 HFRAVDPDGDGHVSWDEYKIKFLASKGFNEKEVAEKLKNNEDLKIDEETQEVLDNLKDRW 203

Query: 159 FNASGANGDGVLDITEFDDFLHPADSKSPKLLLWLCADVVRERDADNDGKLNLSEFFPGI 218
           F A     D +L+  EF  FLHP  S+   +L ++  +++R+ D D D KL LSEF    
Sbjct: 204 FQADNPPPDQLLNEEEFLSFLHPEHSRG--MLKFMVKEIIRDLDQDGDKKLTLSEFIS-- 263

Query: 219 FYLIRSGEEDYGSSDQDDEQMEALAKKVFSVLDKDYGGYVDM 236
             L     E+  + D DD+ +    K+   V+D ++ G V M
Sbjct: 264 --LPVGTVENQQAQDIDDDWVRDRKKEYEEVIDANHDGIVTM 298

[Close]

BLAST of Spg009980 vs. ExPASy Swiss-Prot
Match: Q5BKL9 (45 kDa calcium-binding protein OS=Xenopus tropicalis OX=8364 GN=sdf4 PE=2 SV=1)

HSP 1 Score: 73.2 bits (178), Expect = 4.5e-12
Identity = 62/222 (27.93%), Postives = 99/222 (44.59%), Query Frame = 0


Query: 39  EWEEFINDEDRFNVTERLVSLFPKIDVDPHDGFVSAEELTRWNLQQA---LNEALHRTER 98
           E EEF  D +      +L ++F K+D +  D  +SA E+ RW +++      EA++  + 
Sbjct: 84  EIEEFDEDSEPRRNRRKLAAIFAKVDRN-EDKQISANEMQRWIMEKTEEHFQEAVNENKL 143

Query: 99  EFQSHDENRDGFVSFAEY----------------EPPSCNGSFSYD------LGWWNEEH 158
            F++ D + DG VS+ EY                E    N     D      L    +  
Sbjct: 144 HFRAVDPDGDGHVSWDEYKIKFLASKGFNEKEVAEKLKNNEDLKIDEETQEVLDNLKDRW 203

Query: 159 FNASGANGDGVLDITEFDDFLHPADSKSPKLLLWLCADVVRERDADNDGKLNLSEFFPGI 218
           F A     D +L+  EF  FLHP  S+   +L ++  +++R+ D D D KL LSEF    
Sbjct: 204 FQADNPPADQLLNEEEFLSFLHPEHSQG--MLKFMVKEIIRDLDQDGDKKLTLSEFIS-- 263

Query: 219 FYLIRSGEEDYGSSDQDDEQMEALAKKVFSVLDKDYGGYVDM 236
             L     E+  + D DD+ +    K+   V+D ++ G V M
Sbjct: 264 --LPVGTVENQQAQDIDDDWVRDRKKEYEEVIDANHDGIVTM 298

[Close]

BLAST of Spg009980 vs. ExPASy Swiss-Prot
Match: Q7SXV9 (Calumenin-B OS=Danio rerio OX=7955 GN=calub PE=2 SV=1)

HSP 1 Score: 70.5 bits (171), Expect = 2.9e-11
Identity = 59/227 (25.99%), Postives = 96/227 (42.29%), Query Frame = 0


Query: 27  HHSHSNAADSHPEWEEFINDEDRF---------------NVTERLVSLFPKIDVDPHDGF 86
           HH    +   H + E F  D + F                  ERL  +  KID D HDGF
Sbjct: 29  HHDAPLSNKDHDDEENFDYDHEAFLGQEEAKTFDQLTPEESKERLGKIVEKIDED-HDGF 88

Query: 87  VSAEELTRWNLQQALNEALHRTEREFQSHDENRDGFVSFAEYEPPSC---------NGSF 146
           V+A+E+ RW             +R++Q+HD N D FVS+ EY+  +             F
Sbjct: 89  VTADEMKRWIKHAQRRWIYEDVDRQWQAHDLNSDSFVSWEEYKDATYGYILDEADPEDGF 148

Query: 147 SY-DLGWWNEEHFNASGANGDGVLDITEFDDFLHPADSKSPKLLLWLCADVVRERDADND 206
           +Y  +   +E  F  +  +GD   +  EF  FLHP +    K ++ L  + + + D + D
Sbjct: 149 NYRQMMTRDERRFKMADQDGDLRANKEEFTAFLHPEEFDYMKDIVVL--ETMEDIDKNGD 208

Query: 207 GKLNLSEFFPGIFYLIRSGEEDYGSSDQDDEQMEALAKKVFSVLDKD 229
           G ++L+E+   ++       E      + ++  E   K     +DKD
Sbjct: 209 GLIDLNEYIGDMYSQNGDSSEPEWVKTEREQFTEFRDKNKDGRMDKD 252

[Close]

BLAST of Spg009980 vs. ExPASy TrEMBL
Match: A0A5A7SY06 (Calumenin-B OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G00730 PE=4 SV=1)

HSP 1 Score: 339.7 bits (870), Expect = 9.6e-90
Identity = 163/206 (79.13%), Postives = 177/206 (85.92%), Query Frame = 0


Query: 33  AADSHPEWEEFINDEDRFNVTERLVSLFPKIDVDPHDGFVSAEELTRWNLQQALNEALHR 92
           A DSHPEWEEFINDEDRFNVTERL  +FPKIDVDP DGFVSAEELTRWNLQQA+NEALHR
Sbjct: 82  AHDSHPEWEEFINDEDRFNVTERLFFIFPKIDVDPSDGFVSAEELTRWNLQQAMNEALHR 141

Query: 93  TEREFQSHDENRDGFVSFAEYEPPSC-----NGSFSYDLGWWNEEHFNASGANGDGVLDI 152
           TEREFQSHDENRDGFVSFAEYEPPS      N SF YD+GWWNEEHFNAS A+GDGVLD+
Sbjct: 142 TEREFQSHDENRDGFVSFAEYEPPSWVLAAGNSSFGYDIGWWNEEHFNASDADGDGVLDL 201

Query: 153 TEFDDFLHPADSKSPKLLLWLCADVVRERDADNDGKLNLSEFFPGIFYLIRSGEEDYGSS 212
            EF+DFLHPADSKSPKLLLWLCADVVRERD D DGKLN SEFFP I  LIR  +EDY SS
Sbjct: 202 NEFNDFLHPADSKSPKLLLWLCADVVRERDNDKDGKLNFSEFFPKILDLIRRVDEDYNSS 261

Query: 213 DQDDEQMEALAKKVFSVLDKDYGGYV 234
           + +DE+ EALA+K+F  LDKD  G++
Sbjct: 262 NWEDEEPEALARKMFLELDKDSDGFL 287

[Close]

BLAST of Spg009980 vs. ExPASy TrEMBL
Match: A0A1S3BDS4 (calumenin-B OS=Cucumis melo OX=3656 GN=LOC103488782 PE=4 SV=1)

HSP 1 Score: 339.7 bits (870), Expect = 9.6e-90
Identity = 163/206 (79.13%), Postives = 177/206 (85.92%), Query Frame = 0


Query: 33  AADSHPEWEEFINDEDRFNVTERLVSLFPKIDVDPHDGFVSAEELTRWNLQQALNEALHR 92
           A DSHPEWEEFINDEDRFNVTERL  +FPKIDVDP DGFVSAEELTRWNLQQA+NEALHR
Sbjct: 82  AHDSHPEWEEFINDEDRFNVTERLFFIFPKIDVDPSDGFVSAEELTRWNLQQAMNEALHR 141

Query: 93  TEREFQSHDENRDGFVSFAEYEPPSC-----NGSFSYDLGWWNEEHFNASGANGDGVLDI 152
           TEREFQSHDENRDGFVSFAEYEPPS      N SF YD+GWWNEEHFNAS A+GDGVLD+
Sbjct: 142 TEREFQSHDENRDGFVSFAEYEPPSWVLAAGNSSFGYDIGWWNEEHFNASDADGDGVLDL 201

Query: 153 TEFDDFLHPADSKSPKLLLWLCADVVRERDADNDGKLNLSEFFPGIFYLIRSGEEDYGSS 212
            EF+DFLHPADSKSPKLLLWLCADVVRERD D DGKLN SEFFP I  LIR  +EDY SS
Sbjct: 202 NEFNDFLHPADSKSPKLLLWLCADVVRERDNDKDGKLNFSEFFPKILDLIRRVDEDYNSS 261

Query: 213 DQDDEQMEALAKKVFSVLDKDYGGYV 234
           + +DE+ EALA+K+F  LDKD  G++
Sbjct: 262 NWEDEEPEALARKMFLELDKDSDGFL 287

[Close]

BLAST of Spg009980 vs. ExPASy TrEMBL
Match: A0A0A0KT37 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G579050 PE=4 SV=1)

HSP 1 Score: 337.0 bits (863), Expect = 6.2e-89
Identity = 161/206 (78.16%), Postives = 177/206 (85.92%), Query Frame = 0


Query: 33  AADSHPEWEEFINDEDRFNVTERLVSLFPKIDVDPHDGFVSAEELTRWNLQQALNEALHR 92
           A DSHPEWEEFINDEDRFNVTERL  +FPKIDVDP DGFVSAEELTRWNLQQA+NEAL+R
Sbjct: 82  AHDSHPEWEEFINDEDRFNVTERLFWIFPKIDVDPSDGFVSAEELTRWNLQQAMNEALYR 141

Query: 93  TEREFQSHDENRDGFVSFAEYEPPSC-----NGSFSYDLGWWNEEHFNASGANGDGVLDI 152
           TEREFQSHD+NRDGFVSFAEYEPPS      NGSF YD+GWWNEEHFNAS A+GDGVLD+
Sbjct: 142 TEREFQSHDDNRDGFVSFAEYEPPSWVLSAGNGSFGYDIGWWNEEHFNASDADGDGVLDL 201

Query: 153 TEFDDFLHPADSKSPKLLLWLCADVVRERDADNDGKLNLSEFFPGIFYLIRSGEEDYGSS 212
            EF+DFLHPADSKS KLLLWLCADVVRERD D DGKLN SEFFP +  L+R  +EDY SS
Sbjct: 202 NEFNDFLHPADSKSTKLLLWLCADVVRERDNDKDGKLNFSEFFPKVLDLVRRVDEDYNSS 261

Query: 213 DQDDEQMEALAKKVFSVLDKDYGGYV 234
           + +DE+ EALAKK+F  LDKD  GY+
Sbjct: 262 NWEDEEPEALAKKMFLELDKDSDGYL 287

[Close]

BLAST of Spg009980 vs. ExPASy TrEMBL
Match: A0A6J1KJ28 (reticulocalbin-2-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111493671 PE=4 SV=1)

HSP 1 Score: 321.2 bits (822), Expect = 3.5e-84
Identity = 153/210 (72.86%), Postives = 174/210 (82.86%), Query Frame = 0


Query: 29  SHSNAADSHPEWEEFINDEDRFNVTERLVSLFPKIDVDPHDGFVSAEELTRWNLQQALNE 88
           S S+AADSHPEWEEFINDEDRFNVTERL SLFP IDV+P DGF+S EELTRWN QQA+N 
Sbjct: 82  SQSDAADSHPEWEEFINDEDRFNVTERLSSLFPNIDVNPTDGFISVEELTRWNWQQAMNA 141

Query: 89  ALHRTEREFQSHDENRDGFVSFAEYEPPSC-----NGSFSYDLGWWNEEHFNASGANGDG 148
           ALHRTEREF++HDE+RDGFVSFAEYEPPS      N S  YD+GWWNEEHFNAS A+G+G
Sbjct: 142 ALHRTEREFETHDEDRDGFVSFAEYEPPSWVMAAGNSSSGYDIGWWNEEHFNASDADGNG 201

Query: 149 VLDITEFDDFLHPADSKSPKLLLWLCADVVRERDADNDGKLNLSEFFPGIFYLIRSGEED 208
            LD++EF+DFLHPADS++PKLLLWLC DVVRERD   DGKL+ SEF P IF L+R  +ED
Sbjct: 202 ALDMSEFNDFLHPADSRNPKLLLWLCRDVVRERDNSKDGKLSFSEFSPAIFDLVRRVDED 261

Query: 209 YGSSDQDDEQMEALAKKVFSVLDKDYGGYV 234
           YGS   DD+QMEA AKK+F  LDKD  GY+
Sbjct: 262 YGSLSWDDDQMEARAKKMFLELDKDEDGYL 291

[Close]

BLAST of Spg009980 vs. ExPASy TrEMBL
Match: A0A6J1KGJ7 (calumenin-B-like isoform X4 OS=Cucurbita maxima OX=3661 GN=LOC111493671 PE=4 SV=1)

HSP 1 Score: 321.2 bits (822), Expect = 3.5e-84
Identity = 153/211 (72.51%), Postives = 174/211 (82.46%), Query Frame = 0


Query: 29  SHSNAADSHPEWEEFINDEDRFNVTERLVSLFPKIDVDPHDGFVSAEELTRWNLQQALNE 88
           S S+AADSHPEWEEFINDEDRFNVTERL SLFP IDV+P DGF+S EELTRWN QQA+N 
Sbjct: 82  SQSDAADSHPEWEEFINDEDRFNVTERLSSLFPNIDVNPTDGFISVEELTRWNWQQAMNA 141

Query: 89  ALHRTEREFQSHDENRDGFVSFAEYEPPS------CNGSFSYDLGWWNEEHFNASGANGD 148
           ALHRTEREF++HDE+RDGFVSFAEYEPPS       N S  YD+GWWNEEHFNAS A+G+
Sbjct: 142 ALHRTEREFETHDEDRDGFVSFAEYEPPSWVMAAGSNSSSGYDIGWWNEEHFNASDADGN 201

Query: 149 GVLDITEFDDFLHPADSKSPKLLLWLCADVVRERDADNDGKLNLSEFFPGIFYLIRSGEE 208
           G LD++EF+DFLHPADS++PKLLLWLC DVVRERD   DGKL+ SEF P IF L+R  +E
Sbjct: 202 GALDMSEFNDFLHPADSRNPKLLLWLCRDVVRERDNSKDGKLSFSEFSPAIFDLVRRVDE 261

Query: 209 DYGSSDQDDEQMEALAKKVFSVLDKDYGGYV 234
           DYGS   DD+QMEA AKK+F  LDKD  GY+
Sbjct: 262 DYGSLSWDDDQMEARAKKMFLELDKDEDGYL 292

[Close]

BLAST of Spg009980 vs. TAIR 10
Match: AT5G08580.1 (Calcium-binding EF hand family protein )

HSP 1 Score: 262.3 bits (669), Expect = 3.7e-70
Identity = 122/218 (55.96%), Postives = 160/218 (73.39%), Query Frame = 0


Query: 28  HSHSNAADSHPEWEEF------INDEDRFNVTERLVSLFPKIDVDPHDGFVSAEELTRWN 87
           H H+   +S PEWEEF      +NDE++FNVT+RL+ LFPKIDV P DGF++  ELT W 
Sbjct: 102 HEHAPGHESQPEWEEFMDAEDYLNDEEKFNVTDRLILLFPKIDVSPADGFMTESELTEWT 161

Query: 88  LQQALNEALHRTEREFQSHDENRDGFVSFAEYEPPSC-----NGSFSYDLGWWNEEHFNA 147
           +Q +  E +HRT+R+   HD N+DGF+SF+EYEPPS      N SF YD+GWW EEHFNA
Sbjct: 162 MQSSAKEVVHRTQRDLDVHDRNKDGFISFSEYEPPSWVRKSDNNSFGYDMGWWKEEHFNA 221

Query: 148 SGANGDGVLDITEFDDFLHPADSKSPKLLLWLCADVVRERDADNDGKLNLSEFFPGIFYL 207
           S ANGDG+L++TEF+DFLHPAD+K+PKLLLWLC + VRERD+D DGK++  EFF G+F  
Sbjct: 222 SDANGDGLLNLTEFNDFLHPADTKNPKLLLWLCKEEVRERDSDKDGKISFEEFFHGLFDT 281

Query: 208 IRSGEED-YGSSDQDDEQMEALAKKVFSVLDKDYGGYV 234
           +R+ EED + S+    +  E  AK++FS LDK+  GY+
Sbjct: 282 VRNYEEDNHNSTHPYHDLPEGPAKQLFSQLDKNDDGYL 319

[Close]

BLAST of Spg009980 vs. TAIR 10
Match: AT4G27790.1 (Calcium-binding EF hand family protein )

HSP 1 Score: 121.3 bits (303), Expect = 1.0e-27
Identity = 62/195 (31.79%), Postives = 106/195 (54.36%), Query Frame = 0


Query: 40  WEEFINDEDRFNVTERLVSLFPKIDVDPHDGFVSAEELTRWNLQQALNEALHRTEREFQS 99
           +EE+   E R N T R+  LFP +D  P DGFVS +EL  W +QQ  +  ++RT +E + 
Sbjct: 80  FEEYFAQERRLNTTMRIKFLFPLLDASPRDGFVSLKELQTWMMQQTEDNMVYRTAKELEL 139

Query: 100 HDENRDGFVSFAEYEPP------SCNGSFSYDLGWWNEEHFNASGANGDGVLDITEFDDF 159
            D+++DG ++F EY P         N     + GWW E+ F  S  + +G LDI EF++F
Sbjct: 140 QDKDKDGVITFEEYLPQFSKQDIEKNEKGHGEAGWWMEQ-FKNSDFDHNGSLDIEEFNNF 199

Query: 160 LHPADSKSPKLLLWLCADVVRERDADNDGKLNLSEFFPGIFYLIRSGEEDYGSSDQDDEQ 219
           LHP DS++     W+  + +   D + DGKL   EF    + + +    ++   ++++++
Sbjct: 200 LHPEDSRNGDTQRWVLKERMTGMDTNGDGKLEYKEFVKNAYEMYK----EFAKFEKEEDE 259

Query: 220 MEALAKKVFSVLDKD 229
                + +F+ +D+D
Sbjct: 260 NVPTPQLLFAEMDRD 269

The following BLAST results are available for this feature:

BLAST of Spg009980 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038892509.1	6.8e-90	63.32	calumenin-B-like [Benincasa hispida] >XP_038892510.1 calumenin-B-like [Benincasa...	[more]
XP_008445899.1	2.0e-89	79.13	PREDICTED: calumenin-B [Cucumis melo] >KAA0034085.1 calumenin-B [Cucumis melo va...	[more]
XP_011655504.1	1.3e-88	78.16	calumenin-B [Cucumis sativus] >XP_031741815.1 calumenin-B [Cucumis sativus] >KGN...	[more]
KAG6601072.1	1.5e-84	72.99	45 kDa calcium-binding protein, partial [Cucurbita argyrosperma subsp. sororia]	[more]
KAG7031877.1	5.6e-84	72.51	45 kDa calcium-binding protein, partial [Cucurbita argyrosperma subsp. argyrospe...	[more]

BLAST of Spg009980 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q7ZUC2	1.4e-13	27.93	45 kDa calcium-binding protein OS=Danio rerio OX=7955 GN=sdf4 PE=2 SV=1	[more]
Q5ZKE5	3.1e-13	27.93	45 kDa calcium-binding protein OS=Gallus gallus OX=9031 GN=SDF4 PE=2 SV=2	[more]
Q66JA6	4.5e-12	27.93	45 kDa calcium-binding protein OS=Xenopus laevis OX=8355 GN=sdf4 PE=2 SV=1	[more]
Q5BKL9	4.5e-12	27.93	45 kDa calcium-binding protein OS=Xenopus tropicalis OX=8364 GN=sdf4 PE=2 SV=1	[more]
Q7SXV9	2.9e-11	25.99	Calumenin-B OS=Danio rerio OX=7955 GN=calub PE=2 SV=1	[more]

BLAST of Spg009980 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7SY06	9.6e-90	79.13	Calumenin-B OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G00730 PE=...	[more]
A0A1S3BDS4	9.6e-90	79.13	calumenin-B OS=Cucumis melo OX=3656 GN=LOC103488782 PE=4 SV=1	[more]
A0A0A0KT37	6.2e-89	78.16	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G579050 PE=4 SV=1	[more]
A0A6J1KJ28	3.5e-84	72.86	reticulocalbin-2-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111493671 PE=...	[more]
A0A6J1KGJ7	3.5e-84	72.51	calumenin-B-like isoform X4 OS=Cucurbita maxima OX=3661 GN=LOC111493671 PE=4 SV=...	[more]

BLAST of Spg009980 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G08580.1	3.7e-70	55.96	Calcium-binding EF hand family protein	[more]
AT4G27790.1	1.0e-27	31.79	Calcium-binding EF hand family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 83..103
None	No IPR available	GENE3D	1.10.238.10		coord: 130..236 e-value: 5.0E-9 score: 37.9
None	No IPR available	GENE3D	1.10.238.10		coord: 15..115 e-value: 7.9E-8 score: 34.4
None	No IPR available	PANTHER	PTHR10827:SF83	BNAC09G47400D PROTEIN	coord: 29..233
None	No IPR available	PANTHER	PTHR10827	RETICULOCALBIN	coord: 29..233
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 101..113
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 177..189
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 213..236 score: 8.432208
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 53..193

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg009980.1	Spg009980.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005783	endoplasmic reticulum
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0005509	calcium ion binding