Spg009835 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg009835 vs. NCBI nr
Match: XP_023541587.1 (nuclear pore complex protein NUP214 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 2349.3 bits (6087), Expect = 0.0e+00
Identity = 1386/2032 (68.21%), Postives = 1477/2032 (72.69%), Query Frame = 0

Query: 1    MASVDSRHSTSSTQIPLEDGDEGEHVQTTDYYFEKIGEPVPVKLNDSIIDPESPPSQPLA 60
            MASVDSRHS SST + LED  EGEHV+T DYYFEKIGEPVPVKLNDSI DP SPPSQPLA
Sbjct: 1    MASVDSRHSISSTHVALEDSYEGEHVETNDYYFEKIGEPVPVKLNDSIFDPGSPPSQPLA 60

Query: 61   VSESFGLIFVAHLSGFFVVRTEDVIASAKEMKNGGAGSSVQDLSIVDVSVGKVHILALSS 120
            VSESFGLIFVAHLSGFFVVRT+DV+ASAKEMKNGG GSS+QDLSIVDVSVGKVH+LALS+
Sbjct: 61   VSESFGLIFVAHLSGFFVVRTKDVVASAKEMKNGGTGSSIQDLSIVDVSVGKVHVLALSN 120

Query: 121  DNSILAAVVAGDVHLFSVGSLLDKAEKPSSSHSITDTSCIKDFKWTRKLENSYLVLSKHG 180
            DNS LAAVVAGDVHLFSV SLLDKAEKP  S S TD+SCIKDFKWTRK ENSYLVLSKHG
Sbjct: 121  DNSFLAAVVAGDVHLFSVDSLLDKAEKPYFSCSTTDSSCIKDFKWTRKPENSYLVLSKHG 180

Query: 181  ELYQGSANGPPKHVMHDIDAVECSVKGKFIAVAKKDTLAIFSYKFKERLSMSLLPSLGNG 240
            +LYQGSA+GP KH+MHDIDAVECSVKGKFIAVAKKDT  IFSYKFKERLSMSLLPSLGNG
Sbjct: 181  KLYQGSASGPFKHIMHDIDAVECSVKGKFIAVAKKDTFTIFSYKFKERLSMSLLPSLGNG 240

Query: 241  NTDTDFAVKGSVSVSPSVSLSKNDICKMLKLEVDCIKWVRADCIIIGCFQVTATGDEEDY 300
            +TDTDFAVK                       VD IKWVRADCIIIGCFQVTATGDEEDY
Sbjct: 241  DTDTDFAVK-----------------------VDSIKWVRADCIIIGCFQVTATGDEEDY 300

Query: 301  FVQVVRSKDGKITDVSSNKVLLSFHDIHSGFTHDILPVETGPCLFLSYLDKCKLAIVANR 360
            FVQV+RSKDGKITDVSSNKVLLSFHDI+SGFT DILPVETGPCL LSYLDKCKLAIVANR
Sbjct: 301  FVQVIRSKDGKITDVSSNKVLLSFHDIYSGFTPDILPVETGPCLLLSYLDKCKLAIVANR 360

Query: 361  NNMDQHIVLLGWLLEVENEVAIIDIERDTSLPRINLQVSDRKTMDVLSLLSLIREVVLGP 420
            NN DQHIVLLGWL EVENEVA+IDIERD SLPRI LQ                       
Sbjct: 361  NNTDQHIVLLGWLQEVENEVAVIDIERDKSLPRIELQ----------------------- 420

Query: 421  GRRDVRLWSPNPIEDFSCRLFFRSLLYPSCENGDDNLVMGLCIDRFSLPGKVKVQVGAEE 480
                                          +NGDDNLVMGLCIDR SLPGKV+VQVG EE
Sbjct: 421  ------------------------------DNGDDNLVMGLCIDRVSLPGKVEVQVGNEE 480

Query: 481  TREVSPYCILLCLTLEGKLIMFHFSSVNESEAPHD-VSACDEEEEDGTVEPSDDQSQLSS 540
             REVSPYC LLCLTLEGKLI+FHFSS NESEA  + VSACDEEEED TV P+DDQ QL  
Sbjct: 481  IREVSPYCTLLCLTLEGKLILFHFSSANESEASDETVSACDEEEEDDTVVPTDDQPQLF- 540

Query: 541  ESKNEFREAIVSLKMQDTEKIATNSEIPKEKINISSDIKPSNIDQSSVSNIDESVIVSGE 600
                                                    SNIDQ  VS +DES +++ E
Sbjct: 541  ----------------------------------------SNIDQRPVSKVDESPVITRE 600

Query: 601  SYTKSQKADSFIYSQSLRSSNLERPNNDTGNFDKPVQKFTGLGSVAFSGQSANVPSQSLK 660
            S  KSQ+ DSF +SQ L+ S LERPNN+ GNF KP + FTGLGSVAFSGQS +VPSQ+LK
Sbjct: 601  SNAKSQQMDSFAFSQPLKPSTLERPNNEIGNFAKPAKNFTGLGSVAFSGQSVDVPSQTLK 660

Query: 661  SSILERPNNEIGNFDKPVQKFTGLGSVAFSGQSANVPSQSLKSSILERPNNEIGNFDKPV 720
            SSILERPNNEIGNF+KP  KFTGLGSVAFSGQS ++P+QSLK S LERPNN+IGNF    
Sbjct: 661  SSILERPNNEIGNFNKPFHKFTGLGSVAFSGQSVDMPNQSLKPSFLERPNNQIGNF---- 720

Query: 721  QKFTGFGSVTFSGQSAAMPSQSLKSSILERPNNEIGNCDKPVQKFTGLGSVAFSGQSVDV 780
                                                  DKPVQKFTGLGSVAFS QS DV
Sbjct: 721  --------------------------------------DKPVQKFTGLGSVAFSEQSADV 780

Query: 781  PSQPFLNVKESTKRLGSTGLQAASELSSDKPMLFKKVDPVSSVLPLNSLQSSKTENYGPS 840
            PS PFLNVKEST +                                   QSS        
Sbjct: 781  PSHPFLNVKESTVK-----------------------------------QSS-------- 840

Query: 841  FGAANAFTGFAGKPFQQKDVPSTLTQSERQVTAGSGKIESLPVIRTSQTSLQDNFSTGKT 900
             GAANAFTGFAGKPFQ KDVPSTLTQS RQV+AG+GKIESLPVI++SQ SLQDNFS GK 
Sbjct: 841  -GAANAFTGFAGKPFQPKDVPSTLTQSGRQVSAGAGKIESLPVIQSSQVSLQDNFSLGKI 900

Query: 901  ANEKQDGSDRNYSNVPLAKPMKEMCEGLDKLLESIEEPGGFLDACTAFQNSSVEALELGL 960
            +N+KQDGS+RNY NVPLAKPM EMCEGLD LLESIEEPGGFLDACT FQ SSVEALELGL
Sbjct: 901  SNKKQDGSERNYGNVPLAKPMTEMCEGLDMLLESIEEPGGFLDACTTFQKSSVEALELGL 960

Query: 961  ATLSDQCQIWRRTMNKCAQEVPNLFDKTVQVLQKKTYIEGIVTQASDSNYWEHWDRQKLS 1020
            ATLSDQCQIWRRTM + AQEV  LFD+TV+VL KKTYIEGIVTQASDSNYWEHWDRQKLS
Sbjct: 961  ATLSDQCQIWRRTMTERAQEVQYLFDRTVEVLSKKTYIEGIVTQASDSNYWEHWDRQKLS 1020

Query: 1021 SELELKRQHILKMNQNITNQLIELERHFNGLELNKFGGNDESQVSERALQRKFGSTRHSH 1080
            SELELKRQHIL+MNQN+TNQLIELERHFNGLELNKFGGNDE+QV+ERALQRKFGS+R SH
Sbjct: 1021 SELELKRQHILQMNQNMTNQLIELERHFNGLELNKFGGNDETQVNERALQRKFGSSRQSH 1080

Query: 1081 SLHSLNNIMGSQLAAAQLLSENLSKQMAALNIESPSLKRQSVTKELFETIGITYDASFSS 1140
            SLHSLNNIMGSQLAAAQLLS+NLSKQ+A LNIESPS KRQS+TKELF+TIGITYDASFSS
Sbjct: 1081 SLHSLNNIMGSQLAAAQLLSDNLSKQIATLNIESPSSKRQSITKELFDTIGITYDASFSS 1140

Query: 1141 PNVNKIAETSTSKKLLLSADSFSSKDTSRRKQQSGTKNSEAETGRRRRDSLDRRRETTVG 1200
            PNVNKI ETS SKKLLLSADSFSSKDTSRRKQ+SG K SE ETGRRRRDSLDR       
Sbjct: 1141 PNVNKIPETS-SKKLLLSADSFSSKDTSRRKQRSGAKISETETGRRRRDSLDR------- 1200

Query: 1201 GAVRPDLGSGDGESTVRGGGARAGIRAARFVPLQGLDGGEVSRRQGEEGERRAAEEGERR 1260
                                                                        
Sbjct: 1201 ------------------------------------------------------------ 1260

Query: 1261 AANGRRATSCERAASDERTERARRRRNAEGHIRESHVQNALSYLQELLLYYYGFSVLIRH 1320
                                                                        
Sbjct: 1261 ------------------------------------------------------------ 1320

Query: 1321 VYELEIGFMEMAKNLASVEPPKTTVKRMLLQGIPLSGEKQFRSRTPEGVAAVARPASCIT 1380
                         NLASV+PPKTTV+RM+LQG PLS EK+FRS T EG A VARPAS I 
Sbjct: 1321 -------------NLASVQPPKTTVQRMILQGTPLSNEKEFRSPTLEGPATVARPASRIA 1380

Query: 1381 SSMLSSSSKNAENGSENPATPFTWASPPQPSNSSRQKSQPLQKANATTPSPLPVFQSSHE 1440
            SSMLSSSSKNAE GSENPATPF+WASPP      RQK QP QK N T PSPLPVFQSSHE
Sbjct: 1381 SSMLSSSSKNAEQGSENPATPFSWASPP------RQKFQPPQKTNGTAPSPLPVFQSSHE 1440

Query: 1441 MLKKGTNEAYSVTSENKFAEVTFPEKSKSSDFFSLTRNDSVQKPNMNLDQKSSIFTIPAK 1500
            MLKK  +EAYS  SENKFAEVT+PEKSK+SDFFSL R+DSVQK NMN +QKSSIF   +K
Sbjct: 1441 MLKKSNSEAYSAASENKFAEVTYPEKSKASDFFSLARSDSVQKSNMNFEQKSSIFVTSSK 1500

Query: 1501 QTPTPKDSIDTSNSNSQKTANVKERHTTTSPLFGSANKPESAFVGTASSLVSTVDGARKT 1560
               TPKDSI+T N NSQKTANVKER TT SPLFGSANKPE   VGT SSLV  VDG RKT
Sbjct: 1501 PMSTPKDSIETLNPNSQKTANVKERLTTPSPLFGSANKPEPTSVGTTSSLVPIVDGLRKT 1560

Query: 1561 EEKKSTIAFSPSVPAPALFNTPSSASTLFSGFPVSKSLPS--SAAVIDLNKPGSTSTQLI 1620
            EEKK    FSPSV APA  NTPSSASTLFSG P+SKS PS  +AAV+DLNKP STSTQ  
Sbjct: 1561 EEKKPPTVFSPSVSAPAPVNTPSSASTLFSGSPLSKSFPSPAAAAVVDLNKPLSTSTQSS 1620

Query: 1621 FSSPVVSVSDSLFQAPKMVSPSPTLSSLNPTLDSSKKELPVPKSDTDTEKQASASKPESH 1680
            F+ PVVSVSDSLFQAPKMVSP   LSSLNPTL SS KE P+PKSD DTEKQA ASKPES 
Sbjct: 1621 FAFPVVSVSDSLFQAPKMVSPPSNLSSLNPTLVSSSKEQPMPKSDADTEKQAPASKPESR 1680

Query: 1681 ELKLQPPVTPADKNHVEPTSGTQMVSKDVGGHVPNVIGDAQPQQPSAAFVPLSAPNLTSK 1740
            ELKLQP VT A  NHVEPTS TQ VSKDVGGHVP V  DAQPQQ SAAFVPL  PN T K
Sbjct: 1681 ELKLQPSVTLAVGNHVEPTSVTQTVSKDVGGHVPFVTADAQPQQSSAAFVPLPTPNSTPK 1681

Query: 1741 ISANGKNETSDAVVTQDDDMDEEAPETNNNVEFSLSALGGFG-SSSPISSAPKPNPFGGP 1800
            +SANGK+ETSDA+VTQDDDMDEEAPET NNVEFSLS+LGGFG +S+P+S+APKPNPFGG 
Sbjct: 1741 VSANGKSETSDALVTQDDDMDEEAPET-NNVEFSLSSLGGFGTTSTPMSNAPKPNPFGGS 1681

Query: 1801 FGNVNATSMNSSFTMAPPQSGELFRPASFSFQSPLASQAASQPTNSVAFSGGFGSGMATQ 1860
            FGNVNATSMNSSFTMA P SGELFRPASFSFQSPLASQAASQPTNSVAFSG FGSGMATQ
Sbjct: 1801 FGNVNATSMNSSFTMASPPSGELFRPASFSFQSPLASQAASQPTNSVAFSGSFGSGMATQ 1681

Query: 1861 APSQGGFGQPAQIGVGQQALGNVLGSFGQSRQLGPSLPGTGSGSPGGFS-GGFTSVKPVG 1920
            AP+QGGFGQPAQIGVGQQALG VLGSFGQSRQLGPSLPGT +GSPGGF+ GGFTSVKPVG
Sbjct: 1861 APAQGGFGQPAQIGVGQQALGTVLGSFGQSRQLGPSLPGTATGSPGGFNGGGFTSVKPVG 1681

Query: 1921 GGFAGVGTGGGGGFAGVGSGGGGGFGSVGSGGG--------GGFG---SGGFAGAA---- 1980
            GGFAGVG+GGGGGF G G  GGG  G+  +GGG        GGF     GGFAGAA    
Sbjct: 1921 GGFAGVGSGGGGGFGGGGFSGGGFAGAASTGGGFAGASPPTGGFAGATGGGFAGAAGGGF 1681

Query: 1981 --STGGGFAGAASGFGAFGSQQGSSGFSAFAGGGGGAGGTGKPPELFTQIRK 2011
              + GGGFAGAA GFGAFG+QQGS GFSAF    GG+GGTGKPPELFTQIRK
Sbjct: 1981 AGAAGGGFAGAAGGFGAFGNQQGSGGFSAFGAAPGGSGGTGKPPELFTQIRK 1681

BLAST of Spg009835 vs. NCBI nr
Match: XP_022945174.1 (nuclear pore complex protein NUP214 isoform X2 [Cucurbita moschata])

HSP 1 Score: 2343.2 bits (6071), Expect = 0.0e+00
Identity = 1379/2016 (68.40%), Postives = 1470/2016 (72.92%), Query Frame = 0

Query: 1    MASVDSRHSTSSTQIPLEDGDEGEHVQTTDYYFEKIGEPVPVKLNDSIIDPESPPSQPLA 60
            MASVDSRHS S T I LED  EGEHV+T DYYFEKIGEPVPVKLNDSI DP SPPSQPLA
Sbjct: 1    MASVDSRHSISLTPIALEDSYEGEHVETNDYYFEKIGEPVPVKLNDSIFDPGSPPSQPLA 60

Query: 61   VSESFGLIFVAHLSGFFVVRTEDVIASAKEMKNGGAGSSVQDLSIVDVSVGKVHILALSS 120
            VSESFGLIFVAHLSGFFVVRT DV+ASAKEMKNGG GSS+QDLSIVDVSVGKVH+LALS+
Sbjct: 61   VSESFGLIFVAHLSGFFVVRTTDVVASAKEMKNGGTGSSIQDLSIVDVSVGKVHVLALSN 120

Query: 121  DNSILAAVVAGDVHLFSVGSLLDKAEKPSSSHSITDTSCIKDFKWTRKLENSYLVLSKHG 180
            DNS LAAVVAGDVHLFSV SLLDKAEKP  S S TD+SCIKDFKWTRK ENSYLVLSKHG
Sbjct: 121  DNSFLAAVVAGDVHLFSVDSLLDKAEKPYFSCSTTDSSCIKDFKWTRKPENSYLVLSKHG 180

Query: 181  ELYQGSANGPPKHVMHDIDAVECSVKGKFIAVAKKDTLAIFSYKFKERLSMSLLPSLGNG 240
            +LYQGSA+ P KH+MHDIDAVECSVKGKFIAVAKKDTL+IFSYKFKERLSMSLLPS GNG
Sbjct: 181  KLYQGSASVPFKHIMHDIDAVECSVKGKFIAVAKKDTLSIFSYKFKERLSMSLLPSSGNG 240

Query: 241  NTDTDFAVKGSVSVSPSVSLSKNDICKMLKLEVDCIKWVRADCIIIGCFQVTATGDEEDY 300
            +TDTDFA+K                       VD IKWVRADCIIIGCFQVTATGDEEDY
Sbjct: 241  DTDTDFAMK-----------------------VDSIKWVRADCIIIGCFQVTATGDEEDY 300

Query: 301  FVQVVRSKDGKITDVSSNKVLLSFHDIHSGFTHDILPVETGPCLFLSYLDKCKLAIVANR 360
            FVQV+RSKDGKITDVSSNKVLLSFHDI+SGFT DILPVETGPCL LSYLDKCKLAIVANR
Sbjct: 301  FVQVIRSKDGKITDVSSNKVLLSFHDIYSGFTPDILPVETGPCLLLSYLDKCKLAIVANR 360

Query: 361  NNMDQHIVLLGWLLEVENEVAIIDIERDTSLPRINLQVSDRKTMDVLSLLSLIREVVLGP 420
            NN DQHIVLLGWL EVENEVA+IDIERD SLPRI LQ                       
Sbjct: 361  NNTDQHIVLLGWLQEVENEVAVIDIERDKSLPRIELQ----------------------- 420

Query: 421  GRRDVRLWSPNPIEDFSCRLFFRSLLYPSCENGDDNLVMGLCIDRFSLPGKVKVQVGAEE 480
                                          +NGDDNLVMGLCIDR SLPGKV+VQVG EE
Sbjct: 421  ------------------------------DNGDDNLVMGLCIDRVSLPGKVEVQVGNEE 480

Query: 481  TREVSPYCILLCLTLEGKLIMFHFSSVNESEAPHD-VSACDEEEEDGTVEPSDDQSQLSS 540
             REVSPYC LLCLTLEGKL++FHFSS NESEA  + VSACDEEEED TV P+DDQ QL  
Sbjct: 481  IREVSPYCTLLCLTLEGKLLLFHFSSANESEASDETVSACDEEEEDDTVVPTDDQPQLF- 540

Query: 541  ESKNEFREAIVSLKMQDTEKIATNSEIPKEKINISSDIKPSNIDQSSVSNIDESVIVSGE 600
                                                    SNIDQ  VS +DES +++ E
Sbjct: 541  ----------------------------------------SNIDQRPVSEVDESPVITRE 600

Query: 601  SYTKSQKADSFIYSQSLRSSNLERPNNDTGNFDKPVQKFTGLGSVAFSGQSANVPSQSLK 660
            S  KSQ+ DSF +SQ L+ S LERPNN+ GNF KPV+ FTGLGSVAFSGQS +VPSQSLK
Sbjct: 601  SNAKSQQMDSFAFSQPLKPSTLERPNNEIGNFTKPVKSFTGLGSVAFSGQSVDVPSQSLK 660

Query: 661  SSILERPNNEIGNFDKPVQKFTGLGSVAFSGQSANVPSQSLKSSILERPNNEIGNFDKPV 720
            SSILERPNNEIGNF+KP  KFTGLGSVAFSGQS ++ +QSLK S LERPNN+IGNF    
Sbjct: 661  SSILERPNNEIGNFNKPFHKFTGLGSVAFSGQSVDMANQSLKPSFLERPNNQIGNF---- 720

Query: 721  QKFTGFGSVTFSGQSAAMPSQSLKSSILERPNNEIGNCDKPVQKFTGLGSVAFSGQSVDV 780
                                                  DKPVQKFTGLGSVAFS QS DV
Sbjct: 721  --------------------------------------DKPVQKFTGLGSVAFSEQSADV 780

Query: 781  PSQPFLNVKESTKRLGSTGLQAASELSSDKPMLFKKVDPVSSVLPLNSLQSSKTENYGPS 840
            PS PFLNVKEST +                                   QSS        
Sbjct: 781  PSHPFLNVKESTIK-----------------------------------QSS-------- 840

Query: 841  FGAANAFTGFAGKPFQQKDVPSTLTQSERQVTAGSGKIESLPVIRTSQTSLQDNFSTGKT 900
             GAANAFTGFAGKPFQ KDVPSTLTQS RQV+AG+GKIESLPVI++SQ SLQDNFS GK 
Sbjct: 841  -GAANAFTGFAGKPFQPKDVPSTLTQSGRQVSAGAGKIESLPVIQSSQVSLQDNFSLGKI 900

Query: 901  ANEKQDGSDRNYSNVPLAKPMKEMCEGLDKLLESIEEPGGFLDACTAFQNSSVEALELGL 960
            +N+KQDGS+RNY NVPLAKPM EMCEGLD LLESIEEPGGFLDACT FQ SSVEALELGL
Sbjct: 901  SNKKQDGSERNYGNVPLAKPMTEMCEGLDMLLESIEEPGGFLDACTTFQKSSVEALELGL 960

Query: 961  ATLSDQCQIWRRTMNKCAQEVPNLFDKTVQVLQKKTYIEGIVTQASDSNYWEHWDRQKLS 1020
            ATLSDQCQIWRRTM + AQEV NLFD+TV+VL KKTYIEGIVTQASDSNYWEHWDRQKLS
Sbjct: 961  ATLSDQCQIWRRTMTERAQEVQNLFDRTVEVLSKKTYIEGIVTQASDSNYWEHWDRQKLS 1020

Query: 1021 SELELKRQHILKMNQNITNQLIELERHFNGLELNKFGGNDESQVSERALQRKFGSTRHSH 1080
            SELELKRQ IL+MNQN+TNQLIELERHFNGLELNKFGGN+E QV+ER LQRKFGS+R SH
Sbjct: 1021 SELELKRQRILQMNQNMTNQLIELERHFNGLELNKFGGNEEIQVNERTLQRKFGSSRQSH 1080

Query: 1081 SLHSLNNIMGSQLAAAQLLSENLSKQMAALNIESPSLKRQSVTKELFETIGITYDASFSS 1140
            SLHSLNNIMGSQLAAAQLLS+NLSKQ+A LNIESPS KRQS+TKELFETIGITYDASFSS
Sbjct: 1081 SLHSLNNIMGSQLAAAQLLSDNLSKQIATLNIESPSSKRQSITKELFETIGITYDASFSS 1140

Query: 1141 PNVNKIAETSTSKKLLLSADSFSSKDTSRRKQQSGTKNSEAETGRRRRDSLDRRRETTVG 1200
            PNVNKI ETS SKKLLLSADSFSSKDTSRRKQ+SG K SE ETGRRRRDSLDR       
Sbjct: 1141 PNVNKIPETS-SKKLLLSADSFSSKDTSRRKQRSGAKISETETGRRRRDSLDR------- 1200

Query: 1201 GAVRPDLGSGDGESTVRGGGARAGIRAARFVPLQGLDGGEVSRRQGEEGERRAAEEGERR 1260
                                                                        
Sbjct: 1201 ------------------------------------------------------------ 1260

Query: 1261 AANGRRATSCERAASDERTERARRRRNAEGHIRESHVQNALSYLQELLLYYYGFSVLIRH 1320
                                                                        
Sbjct: 1261 ------------------------------------------------------------ 1320

Query: 1321 VYELEIGFMEMAKNLASVEPPKTTVKRMLLQGIPLSGEKQFRSRTPEGVAAVARPASCIT 1380
                         NLASV+PPKTTVKRM+LQG PLS EKQFRS T EG A +ARPAS I 
Sbjct: 1321 -------------NLASVQPPKTTVKRMILQGTPLSNEKQFRSPTLEGPATIARPASRIP 1380

Query: 1381 SSMLSSSSKNAENGSENPATPFTWASPPQPSNSSRQKSQPLQKANATTPSPLPVFQSSHE 1440
            SSMLSSSSKNAE GS+NPATPF+WASPP      RQK QPLQK N T PS LPVFQSSHE
Sbjct: 1381 SSMLSSSSKNAEQGSKNPATPFSWASPP------RQKFQPLQKTNGTAPSSLPVFQSSHE 1440

Query: 1441 MLKKGTNEAYSVTSENKFAEVTFPEKSKSSDFFSLTRNDSVQKPNMNLDQKSSIFTIPAK 1500
            M+KK  +EAYS  SENKFAEVT+PEKSK+SDFFSL R+DSVQK NMN +QKSSIF   +K
Sbjct: 1441 MVKKSNSEAYSAASENKFAEVTYPEKSKASDFFSLARSDSVQKSNMNFEQKSSIFVTSSK 1500

Query: 1501 QTPTPKDSIDTSNSNSQKTANVKERHTTTSPLFGSANKPESAFVGTASSLVSTVDGARKT 1560
               TPKDSI+T N NSQKTANVKER TT SPLFGSANKPE   VGT SSLV TVDG RKT
Sbjct: 1501 PMSTPKDSIETLNPNSQKTANVKERLTTPSPLFGSANKPEPVSVGTTSSLVPTVDGLRKT 1560

Query: 1561 EEKKSTIAFSPSVPAPALFNTPSSASTLFSGFPVSKSLPSSAAVIDLNKPGSTSTQLIFS 1620
            EEKK    FSPSVPAPA  NTP SASTLFSG P+SKS PS AAV+DLNKP STSTQ  F+
Sbjct: 1561 EEKKPPTVFSPSVPAPAPVNTPPSASTLFSGSPLSKSFPSPAAVVDLNKPLSTSTQSSFA 1620

Query: 1621 SPVVSVSDSLFQAPKMVSPSPTLSSLNPTLDSSKKELPVPKSDTDTEKQASASKPESHEL 1680
            SPVVSVSDSLFQAPKMVSP   LSSLNPTL SS KE P+PKSD DTEKQA ASKPES EL
Sbjct: 1621 SPVVSVSDSLFQAPKMVSPPSNLSSLNPTLVSSSKEQPMPKSDADTEKQAPASKPESREL 1663

Query: 1681 KLQPPVTPADKNHVEPTSGTQMVSKDVGGHVPNVIGDAQPQQPSAAFVPLSAPNLTSKIS 1740
            KLQP VT A  NHVEPTS TQ VSKDVGGHVP V+ DAQPQQ  AAFVPL  PN TSK +
Sbjct: 1681 KLQPSVTLAVGNHVEPTSVTQTVSKDVGGHVPFVVADAQPQQSPAAFVPLPTPNSTSKAA 1663

Query: 1741 ANGKNETSDAVVTQDDDMDEEAPETNNNVEFSLSALGGFG-SSSPISSAPKPNPFGGPFG 1800
            ANGK+ETSDA++TQDDDMDEEAPET NNVEFSLS+LGGFG +S+P+S+APKPNPFGG FG
Sbjct: 1741 ANGKSETSDALITQDDDMDEEAPET-NNVEFSLSSLGGFGTTSTPMSNAPKPNPFGGSFG 1663

Query: 1801 NVNATSMNSSFTMAPPQSGELFRPASFSFQSPLASQAASQPTNSVAFSGGFGSGMATQAP 1860
            NVNATSMNSSFT A P SGELFRPASFSFQSPLASQAASQPTN+VAFSG FGSGMATQAP
Sbjct: 1801 NVNATSMNSSFTTASPPSGELFRPASFSFQSPLASQAASQPTNAVAFSGSFGSGMATQAP 1663

Query: 1861 SQGGFGQPAQIGVGQQALGNVLGSFGQSRQLGPSLPGTGSGSPGGFS-GGFTSVKPVGGG 1920
            +QGGFGQPAQIGVGQQALG VLGSFGQSRQLGPSLPGT SGSPGGF+ GGFTSVKPVGGG
Sbjct: 1861 AQGGFGQPAQIGVGQQALGTVLGSFGQSRQLGPSLPGTASGSPGGFNGGGFTSVKPVGGG 1663

Query: 1921 FAGVGTGGGGGFAGVGSGGGGGFGSVGSGGGGGFGS---GGFAGAASTGGGFAGAASGFG 1980
            FAGVG+GGGGGF G G  GGG  G+  +GGG    S   GGFAGAA  GGGFAGAA GFG
Sbjct: 1921 FAGVGSGGGGGFGGGGFSGGGFAGAASTGGGFAGASPPTGGFAGAA--GGGFAGAAGGFG 1663

Query: 1981 AFGSQQGSSGFSAFAGGGGGAGGTGKPPELFTQIRK 2011
            AFG+QQGS GFSAF    GG+GGTGKPPELFTQIRK
Sbjct: 1981 AFGNQQGSGGFSAFGAAPGGSGGTGKPPELFTQIRK 1663

BLAST of Spg009835 vs. NCBI nr
Match: XP_022945173.1 (nuclear pore complex protein NUP214 isoform X1 [Cucurbita moschata])

HSP 1 Score: 2342.0 bits (6068), Expect = 0.0e+00
Identity = 1381/2030 (68.03%), Postives = 1473/2030 (72.56%), Query Frame = 0

Query: 1    MASVDSRHSTSSTQIPLEDGDEGEHVQTTDYYFEKIGEPVPVKLNDSIIDPESPPSQPLA 60
            MASVDSRHS S T I LED  EGEHV+T DYYFEKIGEPVPVKLNDSI DP SPPSQPLA
Sbjct: 1    MASVDSRHSISLTPIALEDSYEGEHVETNDYYFEKIGEPVPVKLNDSIFDPGSPPSQPLA 60

Query: 61   VSESFGLIFVAHLSGFFVVRTEDVIASAKEMKNGGAGSSVQDLSIVDVSVGKVHILALSS 120
            VSESFGLIFVAHLSGFFVVRT DV+ASAKEMKNGG GSS+QDLSIVDVSVGKVH+LALS+
Sbjct: 61   VSESFGLIFVAHLSGFFVVRTTDVVASAKEMKNGGTGSSIQDLSIVDVSVGKVHVLALSN 120

Query: 121  DNSILAAVVAGDVHLFSVGSLLDKAEKPSSSHSITDTSCIKDFKWTRKLENSYLVLSKHG 180
            DNS LAAVVAGDVHLFSV SLLDKAEKP  S S TD+SCIKDFKWTRK ENSYLVLSKHG
Sbjct: 121  DNSFLAAVVAGDVHLFSVDSLLDKAEKPYFSCSTTDSSCIKDFKWTRKPENSYLVLSKHG 180

Query: 181  ELYQGSANGPPKHVMHDIDAVECSVKGKFIAVAKKDTLAIFSYKFKERLSMSLLPSLGNG 240
            +LYQGSA+ P KH+MHDIDAVECSVKGKFIAVAKKDTL+IFSYKFKERLSMSLLPS GNG
Sbjct: 181  KLYQGSASVPFKHIMHDIDAVECSVKGKFIAVAKKDTLSIFSYKFKERLSMSLLPSSGNG 240

Query: 241  NTDTDFAVKGSVSVSPSVSLSKNDICKMLKLEVDCIKWVRADCIIIGCFQVTATGDEEDY 300
            +TDTDFA+K                       VD IKWVRADCIIIGCFQVTATGDEEDY
Sbjct: 241  DTDTDFAMK-----------------------VDSIKWVRADCIIIGCFQVTATGDEEDY 300

Query: 301  FVQVVRSKDGKITDVSSNKVLLSFHDIHSGFTHDILPVETGPCLFLSYLDKCKLAIVANR 360
            FVQV+RSKDGKITDVSSNKVLLSFHDI+SGFT DILPVETGPCL LSYLDKCKLAIVANR
Sbjct: 301  FVQVIRSKDGKITDVSSNKVLLSFHDIYSGFTPDILPVETGPCLLLSYLDKCKLAIVANR 360

Query: 361  NNMDQHIVLLGWLLEVENEVAIIDIERDTSLPRINLQVSDRKTMDVLSLLSLIREVVLGP 420
            NN DQHIVLLGWL EVENEVA+IDIERD SLPRI LQ                       
Sbjct: 361  NNTDQHIVLLGWLQEVENEVAVIDIERDKSLPRIELQ----------------------- 420

Query: 421  GRRDVRLWSPNPIEDFSCRLFFRSLLYPSCENGDDNLVMGLCIDRFSLPGKVKVQVGAEE 480
                                          +NGDDNLVMGLCIDR SLPGKV+VQVG EE
Sbjct: 421  ------------------------------DNGDDNLVMGLCIDRVSLPGKVEVQVGNEE 480

Query: 481  TREVSPYCILLCLTLEGKLIMFHFSSVNESEAPHD-VSACDEEEEDGTVEPSDDQSQLSS 540
             REVSPYC LLCLTLEGKL++FHFSS NESEA  + VSACDEEEED TV P+DDQ QL  
Sbjct: 481  IREVSPYCTLLCLTLEGKLLLFHFSSANESEASDETVSACDEEEEDDTVVPTDDQPQLF- 540

Query: 541  ESKNEFREAIVSLKMQDTEKIATNSEIPKEKINISSDIKPSNIDQSSVSNIDESVIVSGE 600
                                                    SNIDQ  VS +DES +++ E
Sbjct: 541  ----------------------------------------SNIDQRPVSEVDESPVITRE 600

Query: 601  SYTKSQKADSFIYSQSLRSSNLERPNNDTGNFDKPVQKFTGLGSVAFSGQSANVPSQSLK 660
            S  KSQ+ DSF +SQ L+ S LERPNN+ GNF KPV+ FTGLGSVAFSGQS +VPSQSLK
Sbjct: 601  SNAKSQQMDSFAFSQPLKPSTLERPNNEIGNFTKPVKSFTGLGSVAFSGQSVDVPSQSLK 660

Query: 661  SSILERPNNEIGNFDKPVQKFTGLGSVAFSGQSANVPSQSLKSSILERPNNEIGNFDKPV 720
            SSILERPNNEIGNF+KP  KFTGLGSVAFSGQS ++ +QSLK S LERPNN+IGNF    
Sbjct: 661  SSILERPNNEIGNFNKPFHKFTGLGSVAFSGQSVDMANQSLKPSFLERPNNQIGNF---- 720

Query: 721  QKFTGFGSVTFSGQSAAMPSQSLKSSILERPNNEIGNCDKPVQKFTGLGSVAFSGQSVDV 780
                                                  DKPVQKFTGLGSVAFS QS DV
Sbjct: 721  --------------------------------------DKPVQKFTGLGSVAFSEQSADV 780

Query: 781  PSQPFLNVKESTKRLGSTGLQAASELSSDKPMLFKKVDPVSSVLPLNSLQSSKTENYGPS 840
            PS PFLNVKEST +                                   QSS        
Sbjct: 781  PSHPFLNVKESTIK-----------------------------------QSS-------- 840

Query: 841  FGAANAFTGFAGKPFQQKDVPSTLTQSERQVTAGSGKIESLPVIRTSQTSLQDNFSTGKT 900
             GAANAFTGFAGKPFQ KDVPSTLTQS RQV+AG+GKIESLPVI++SQ SLQDNFS GK 
Sbjct: 841  -GAANAFTGFAGKPFQPKDVPSTLTQSGRQVSAGAGKIESLPVIQSSQVSLQDNFSLGKI 900

Query: 901  ANEKQDGSDRNYSNVPLAKPMKEMCEGLDKLLESIEEPGGFLDACTAFQNSSVEALELGL 960
            +N+KQDGS+RNY NVPLAKPM EMCEGLD LLESIEEPGGFLDACT FQ SSVEALELGL
Sbjct: 901  SNKKQDGSERNYGNVPLAKPMTEMCEGLDMLLESIEEPGGFLDACTTFQKSSVEALELGL 960

Query: 961  ATLSDQCQIWRRTMNKCAQEVPNLFDKTVQVLQKKTYIEGIVTQASDSNYWEHWDRQKLS 1020
            ATLSDQCQIWRRTM + AQEV NLFD+TV+VL KKTYIEGIVTQASDSNYWEHWDRQKLS
Sbjct: 961  ATLSDQCQIWRRTMTERAQEVQNLFDRTVEVLSKKTYIEGIVTQASDSNYWEHWDRQKLS 1020

Query: 1021 SELELKRQHILKMNQNITNQLIELERHFNGLELNKFGGNDESQVSERALQRKFGSTRHSH 1080
            SELELKRQ IL+MNQN+TNQLIELERHFNGLELNKFGGN+E QV+ER LQRKFGS+R SH
Sbjct: 1021 SELELKRQRILQMNQNMTNQLIELERHFNGLELNKFGGNEEIQVNERTLQRKFGSSRQSH 1080

Query: 1081 SLHSLNNIMGSQLAAAQLLSENLSKQMAALNIESPSLKRQSVTKELFETIGITYDASFSS 1140
            SLHSLNNIMGSQLAAAQLLS+NLSKQ+A LNIESPS KRQS+TKELFETIGITYDASFSS
Sbjct: 1081 SLHSLNNIMGSQLAAAQLLSDNLSKQIATLNIESPSSKRQSITKELFETIGITYDASFSS 1140

Query: 1141 PNVNKIAETSTSKKLLLSADSFSSKDTSRRKQQSGTKNSEAETGRRRRDSLDRRRETTVG 1200
            PNVNKI ETS SKKLLLSADSFSSKDTSRRKQ+SG K SE ETGRRRRDSLDR       
Sbjct: 1141 PNVNKIPETS-SKKLLLSADSFSSKDTSRRKQRSGAKISETETGRRRRDSLDR------- 1200

Query: 1201 GAVRPDLGSGDGESTVRGGGARAGIRAARFVPLQGLDGGEVSRRQGEEGERRAAEEGERR 1260
                                                                        
Sbjct: 1201 ------------------------------------------------------------ 1260

Query: 1261 AANGRRATSCERAASDERTERARRRRNAEGHIRESHVQNALSYLQELLLYYYGFSVLIRH 1320
                                                                        
Sbjct: 1261 ------------------------------------------------------------ 1320

Query: 1321 VYELEIGFMEMAKNLASVEPPKTTVKRMLLQGIPLSGEKQFRSRTPEGVAAVARPASCIT 1380
                         NLASV+PPKTTVKRM+LQG PLS EKQFRS T EG A +ARPAS I 
Sbjct: 1321 -------------NLASVQPPKTTVKRMILQGTPLSNEKQFRSPTLEGPATIARPASRIP 1380

Query: 1381 SSMLSSSSKNAENGSENPATPFTWASPPQPSNSSRQKSQPLQKANATTPSPLPVFQSSHE 1440
            SSMLSSSSKNAE GS+NPATPF+WASPP      RQK QPLQK N T PS LPVFQSSHE
Sbjct: 1381 SSMLSSSSKNAEQGSKNPATPFSWASPP------RQKFQPLQKTNGTAPSSLPVFQSSHE 1440

Query: 1441 MLKKGTNEAYSVTSENKFAEVTFPEKSKSSDFFSLTRNDSVQKPNMNLDQKSSIFTIPAK 1500
            M+KK  +EAYS  SENKFAEVT+PEKSK+SDFFSL R+DSVQK NMN +QKSSIF   +K
Sbjct: 1441 MVKKSNSEAYSAASENKFAEVTYPEKSKASDFFSLARSDSVQKSNMNFEQKSSIFVTSSK 1500

Query: 1501 QTPTPKDSIDTSNSNSQKTANVKERHTTTSPLFGSANKPESAFVGTASSLVSTVDGARKT 1560
               TPKDSI+T N NSQKTANVKER TT SPLFGSANKPE   VGT SSLV TVDG RKT
Sbjct: 1501 PMSTPKDSIETLNPNSQKTANVKERLTTPSPLFGSANKPEPVSVGTTSSLVPTVDGLRKT 1560

Query: 1561 EEKKSTIAFSPSVPAPALFNTPSSASTLFSGFPVSKSLPSSAAVIDLNKPGSTSTQLIFS 1620
            EEKK    FSPSVPAPA  NTP SASTLFSG P+SKS PS AAV+DLNKP STSTQ  F+
Sbjct: 1561 EEKKPPTVFSPSVPAPAPVNTPPSASTLFSGSPLSKSFPSPAAVVDLNKPLSTSTQSSFA 1620

Query: 1621 SPVVSVSDSLFQAPKMVSPSPTLSSLNPTLDSSKKELPVPKSDTDTEKQASASKPESHEL 1680
            SPVVSVSDSLFQAPKMVSP   LSSLNPTL SS KE P+PKSD DTEKQA ASKPES EL
Sbjct: 1621 SPVVSVSDSLFQAPKMVSPPSNLSSLNPTLVSSSKEQPMPKSDADTEKQAPASKPESREL 1679

Query: 1681 KLQPPVTPADKNHVEPTSGTQMVSKDVGGHVPNVIGDAQPQQPSAAFVPLSAPNLTSKIS 1740
            KLQP VT A  NHVEPTS TQ VSKDVGGHVP V+ DAQPQQ  AAFVPL  PN TSK +
Sbjct: 1681 KLQPSVTLAVGNHVEPTSVTQTVSKDVGGHVPFVVADAQPQQSPAAFVPLPTPNSTSKAA 1679

Query: 1741 ANGKNETSDAVVTQDDDMDEEAPETNNNVEFSLSALGGFG-SSSPISSAPKPNPFGGPFG 1800
            ANGK+ETSDA++TQDDDMDEEAPET NNVEFSLS+LGGFG +S+P+S+APKPNPFGG FG
Sbjct: 1741 ANGKSETSDALITQDDDMDEEAPET-NNVEFSLSSLGGFGTTSTPMSNAPKPNPFGGSFG 1679

Query: 1801 NVNATSMNSSFTMAPPQSGELFRPASFSFQSPLASQAASQPTNSVAFSGGFGSGMATQAP 1860
            NVNATSMNSSFT A P SGELFRPASFSFQSPLASQAASQPTN+VAFSG FGSGMATQAP
Sbjct: 1801 NVNATSMNSSFTTASPPSGELFRPASFSFQSPLASQAASQPTNAVAFSGSFGSGMATQAP 1679

Query: 1861 SQGGFGQPAQIGVGQQALGNVLGSFGQSRQLGPSLPGTGSGSPGGFS-GGFTSVKPVGGG 1920
            +QGGFGQPAQIGVGQQALG VLGSFGQSRQLGPSLPGT SGSPGGF+ GGFTSVKPVGGG
Sbjct: 1861 AQGGFGQPAQIGVGQQALGTVLGSFGQSRQLGPSLPGTASGSPGGFNGGGFTSVKPVGGG 1679

Query: 1921 FAGVGTGGGGGFAGVGSGGGGGFGSVGSGGG--------GGFG---SGGFAGAA------ 1980
            FAGVG+GGGGGF G G  GGG  G+  +GGG        GGF     GGFAGAA      
Sbjct: 1921 FAGVGSGGGGGFGGGGFSGGGFAGAASTGGGFAGASPPTGGFAGATGGGFAGAAGGGFAG 1679

Query: 1981 STGGGFAGAASGFGAFGSQQGSSGFSAFAGGGGGAGGTGKPPELFTQIRK 2011
            + GGGFAGAA GFGAFG+QQGS GFSAF    GG+GGTGKPPELFTQIRK
Sbjct: 1981 AAGGGFAGAAGGFGAFGNQQGSGGFSAFGAAPGGSGGTGKPPELFTQIRK 1679

BLAST of Spg009835 vs. NCBI nr
Match: XP_022966767.1 (nuclear pore complex protein NUP214 isoform X3 [Cucurbita maxima])

HSP 1 Score: 2340.8 bits (6065), Expect = 0.0e+00
Identity = 1372/2016 (68.06%), Postives = 1468/2016 (72.82%), Query Frame = 0

Query: 1    MASVDSRHSTSSTQIPLEDGDEGEHVQTTDYYFEKIGEPVPVKLNDSIIDPESPPSQPLA 60
            MASVDSRHSTSST IPLED  EGEHV+T DYYFEKIGEPVPVKLNDSI DP SPPSQPLA
Sbjct: 1    MASVDSRHSTSSTPIPLEDSYEGEHVETNDYYFEKIGEPVPVKLNDSIFDPGSPPSQPLA 60

Query: 61   VSESFGLIFVAHLSGFFVVRTEDVIASAKEMKNGGAGSSVQDLSIVDVSVGKVHILALSS 120
            VSESFGLIFVAHLSGFF VRT+DV+ASAKEMKNGG GSS+QDLSIVDVSVGKVH+LALS+
Sbjct: 61   VSESFGLIFVAHLSGFFAVRTKDVVASAKEMKNGGTGSSIQDLSIVDVSVGKVHVLALSN 120

Query: 121  DNSILAAVVAGDVHLFSVGSLLDKAEKPSSSHSITDTSCIKDFKWTRKLENSYLVLSKHG 180
            DNS LAAVVAGDVHLF V SLLDK E+PS S S TD+SCIKDFKWTRK ENSYLVLSKHG
Sbjct: 121  DNSFLAAVVAGDVHLFLVDSLLDKGEEPSFSCSTTDSSCIKDFKWTRKPENSYLVLSKHG 180

Query: 181  ELYQGSANGPPKHVMHDIDAVECSVKGKFIAVAKKDTLAIFSYKFKERLSMSLLPSLGNG 240
            +LYQGSA+GP KH+MHDIDAVECSVKGKFIAVAKKDTL +FSYKFKERLSMSLLPSLGNG
Sbjct: 181  KLYQGSASGPFKHIMHDIDAVECSVKGKFIAVAKKDTLTVFSYKFKERLSMSLLPSLGNG 240

Query: 241  NTDTDFAVKGSVSVSPSVSLSKNDICKMLKLEVDCIKWVRADCIIIGCFQVTATGDEEDY 300
            +TDTDFAVK                       VD IKWVRADCIIIGCFQVTATGDEEDY
Sbjct: 241  DTDTDFAVK-----------------------VDSIKWVRADCIIIGCFQVTATGDEEDY 300

Query: 301  FVQVVRSKDGKITDVSSNKVLLSFHDIHSGFTHDILPVETGPCLFLSYLDKCKLAIVANR 360
            FVQV+RSKDGKITDVSSNKVLLSFHDI+SGFT DILPVETGPCL LSYLDKCKLAIVANR
Sbjct: 301  FVQVIRSKDGKITDVSSNKVLLSFHDIYSGFTPDILPVETGPCLLLSYLDKCKLAIVANR 360

Query: 361  NNMDQHIVLLGWLLEVENEVAIIDIERDTSLPRINLQVSDRKTMDVLSLLSLIREVVLGP 420
            NN DQHIVLLGWL EVENEVA+IDIERD SLPRI LQ                       
Sbjct: 361  NNTDQHIVLLGWLQEVENEVAVIDIERDKSLPRIELQ----------------------- 420

Query: 421  GRRDVRLWSPNPIEDFSCRLFFRSLLYPSCENGDDNLVMGLCIDRFSLPGKVKVQVGAEE 480
                                          +NGDDNLVMGLCIDR SLPGKV+VQVG EE
Sbjct: 421  ------------------------------DNGDDNLVMGLCIDRVSLPGKVEVQVGNEE 480

Query: 481  TREVSPYCILLCLTLEGKLIMFHFSSVNESEAPHD-VSACDEEEEDGTVEPSDDQSQLSS 540
             REVSPYC LLCLTLEGKLI+FHFSS NESEA  + VSACDEEEED TV P+DDQ QL  
Sbjct: 481  IREVSPYCTLLCLTLEGKLILFHFSSANESEASDETVSACDEEEEDETVVPTDDQPQLF- 540

Query: 541  ESKNEFREAIVSLKMQDTEKIATNSEIPKEKINISSDIKPSNIDQSSVSNIDESVIVSGE 600
                                                    SNIDQ  VS +D S +++ E
Sbjct: 541  ----------------------------------------SNIDQRPVSKVDGSPVITRE 600

Query: 601  SYTKSQKADSFIYSQSLRSSNLERPNNDTGNFDKPVQKFTGLGSVAFSGQSANVPSQSLK 660
            S  KSQ+ DS  +SQ L+ S LERPNN+ GNF KPV+ FTGLGSVAFSGQS +VPSQ LK
Sbjct: 601  SNAKSQQMDSLAFSQPLKPSTLERPNNEIGNFAKPVKNFTGLGSVAFSGQSVDVPSQPLK 660

Query: 661  SSILERPNNEIGNFDKPVQKFTGLGSVAFSGQSANVPSQSLKSSILERPNNEIGNFDKPV 720
            SSILERPNNEIGNF+KP  KFTGLGSVAFSGQS ++P++SLK S LERPNN+IGNF    
Sbjct: 661  SSILERPNNEIGNFNKPFHKFTGLGSVAFSGQSVDMPNESLKPSFLERPNNQIGNF---- 720

Query: 721  QKFTGFGSVTFSGQSAAMPSQSLKSSILERPNNEIGNCDKPVQKFTGLGSVAFSGQSVDV 780
                                                  DKPVQKFTGLGSVAFS QSVDV
Sbjct: 721  --------------------------------------DKPVQKFTGLGSVAFSEQSVDV 780

Query: 781  PSQPFLNVKESTKRLGSTGLQAASELSSDKPMLFKKVDPVSSVLPLNSLQSSKTENYGPS 840
            PS PFLNVKEST +  S                                           
Sbjct: 781  PSHPFLNVKESTIKHSS------------------------------------------- 840

Query: 841  FGAANAFTGFAGKPFQQKDVPSTLTQSERQVTAGSGKIESLPVIRTSQTSLQDNFSTGKT 900
             GAANAFTGFAGKPFQ KDVPSTLTQS RQV+AG+GKIESLPVI++SQ SLQDNFS GK 
Sbjct: 841  -GAANAFTGFAGKPFQPKDVPSTLTQSGRQVSAGAGKIESLPVIQSSQVSLQDNFSLGKI 900

Query: 901  ANEKQDGSDRNYSNVPLAKPMKEMCEGLDKLLESIEEPGGFLDACTAFQNSSVEALELGL 960
            +N+KQDGS+RNY NVPLAKPM EMCEGLD LLESIEEPGGFLDACT FQ SSVEAL LGL
Sbjct: 901  SNKKQDGSERNYGNVPLAKPMNEMCEGLDMLLESIEEPGGFLDACTTFQKSSVEALGLGL 960

Query: 961  ATLSDQCQIWRRTMNKCAQEVPNLFDKTVQVLQKKTYIEGIVTQASDSNYWEHWDRQKLS 1020
            ATLSDQCQIWRRTM + AQEV NLFD+TV+VL KKTYIEGIVTQASDSNYW+HWDRQKLS
Sbjct: 961  ATLSDQCQIWRRTMTERAQEVQNLFDRTVEVLSKKTYIEGIVTQASDSNYWDHWDRQKLS 1020

Query: 1021 SELELKRQHILKMNQNITNQLIELERHFNGLELNKFGGNDESQVSERALQRKFGSTRHSH 1080
            SELELKRQ IL+MNQN+TNQLIELERHFNGLELN FGGN+E QV+ER LQRKFGS+R SH
Sbjct: 1021 SELELKRQRILQMNQNMTNQLIELERHFNGLELNTFGGNEEIQVNERTLQRKFGSSRQSH 1080

Query: 1081 SLHSLNNIMGSQLAAAQLLSENLSKQMAALNIESPSLKRQSVTKELFETIGITYDASFSS 1140
            SLHSLNNIMGSQLAAAQLLS+NLSKQ+A LNI+SPS KRQS+TKELFETIGITYDASFSS
Sbjct: 1081 SLHSLNNIMGSQLAAAQLLSDNLSKQIATLNIKSPSSKRQSITKELFETIGITYDASFSS 1140

Query: 1141 PNVNKIAETSTSKKLLLSADSFSSKDTSRRKQQSGTKNSEAETGRRRRDSLDRRRETTVG 1200
            PNVNKI ETS SKKLLLSADSFSSKDTSRRKQ+SG K SE ETGRRRRDSLDR       
Sbjct: 1141 PNVNKIPETS-SKKLLLSADSFSSKDTSRRKQRSGAKISETETGRRRRDSLDR------- 1200

Query: 1201 GAVRPDLGSGDGESTVRGGGARAGIRAARFVPLQGLDGGEVSRRQGEEGERRAAEEGERR 1260
                                                                        
Sbjct: 1201 ------------------------------------------------------------ 1260

Query: 1261 AANGRRATSCERAASDERTERARRRRNAEGHIRESHVQNALSYLQELLLYYYGFSVLIRH 1320
                                                                        
Sbjct: 1261 ------------------------------------------------------------ 1320

Query: 1321 VYELEIGFMEMAKNLASVEPPKTTVKRMLLQGIPLSGEKQFRSRTPEGVAAVARPASCIT 1380
                         NLAS++PPKTTVKRM+LQG PLS EKQFRS T EG A VARPA  I 
Sbjct: 1321 -------------NLASIQPPKTTVKRMILQGTPLSNEKQFRSPTLEGPATVARPAGRIP 1380

Query: 1381 SSMLSSSSKNAENGSENPATPFTWASPPQPSNSSRQKSQPLQKANATTPSPLPVFQSSHE 1440
            SSMLSSSSKNAE GSENPATPF+WASPP      RQK QPLQK N T PSPLPV+QSSHE
Sbjct: 1381 SSMLSSSSKNAEQGSENPATPFSWASPP------RQKFQPLQKTNGTAPSPLPVYQSSHE 1440

Query: 1441 MLKKGTNEAYSVTSENKFAEVTFPEKSKSSDFFSLTRNDSVQKPNMNLDQKSSIFTIPAK 1500
            M+KK  +EAYS  SENKFAEVT+PEKSK+SDFFSL R+DSVQK NMN +QKSS F   +K
Sbjct: 1441 MVKKSNSEAYSAASENKFAEVTYPEKSKASDFFSLARSDSVQKSNMNFEQKSSFFVTSSK 1500

Query: 1501 QTPTPKDSIDTSNSNSQKTANVKERHTTTSPLFGSANKPESAFVGTASSLVSTVDGARKT 1560
               TPKDSI+T N NSQKTANVKER TT SPLFG+ANKPE A VGT SSLV TVD  RKT
Sbjct: 1501 PMSTPKDSIETLNPNSQKTANVKERLTTPSPLFGAANKPEPASVGTTSSLVPTVDELRKT 1560

Query: 1561 EEKKSTIAFSPSVPAPALFNTPSSASTLFSGFPVSKSLPSSAAVIDLNKPGSTSTQLIFS 1620
            EEKK    FSPSVPA    NTPSSASTLFSG P+SKS PS AAV+DLNKP STSTQ  F+
Sbjct: 1561 EEKKPPTVFSPSVPASVPVNTPSSASTLFSGSPLSKSFPSPAAVVDLNKPLSTSTQSSFA 1620

Query: 1621 SPVVSVSDSLFQAPKMVSPSPTLSSLNPTLDSSKKELPVPKSDTDTEKQASASKPESHEL 1680
            SPVVSVSDSLFQAPKMVSP  TLSSLNP+L SS KE P+PKSD DTEKQA ASKPE  EL
Sbjct: 1621 SPVVSVSDSLFQAPKMVSPPSTLSSLNPSLVSSSKEQPIPKSDADTEKQAQASKPEFREL 1663

Query: 1681 KLQPPVTPADKNHVEPTSGTQMVSKDVGGHVPNVIGDAQPQQPSAAFVPLSAPNLTSKIS 1740
            KLQP VT A  NHVEPTS TQ VSKDVGGHVP+VI DAQPQQ SAAFVPL +PN T K+S
Sbjct: 1681 KLQPSVTLAVGNHVEPTSVTQTVSKDVGGHVPSVIADAQPQQSSAAFVPLPSPNSTPKVS 1663

Query: 1741 ANGKNETSDAVVTQDDDMDEEAPETNNNVEFSLSALGGFG-SSSPISSAPKPNPFGGPFG 1800
            ANGK+ETSDA++TQDDDMDEEAPET NNVEFSLS+LGGFG +S+P+S+APKPNPFGG FG
Sbjct: 1741 ANGKSETSDALITQDDDMDEEAPET-NNVEFSLSSLGGFGTTSTPMSNAPKPNPFGGSFG 1663

Query: 1801 NVNATSMNSSFTMAPPQSGELFRPASFSFQSPLASQAASQPTNSVAFSGGFGSGMATQAP 1860
            N NATSMNSSFT A P SGELFRPASFSFQSPLASQAASQPTNSVAFS  FGSGMATQAP
Sbjct: 1801 NANATSMNSSFTTASPPSGELFRPASFSFQSPLASQAASQPTNSVAFSSSFGSGMATQAP 1663

Query: 1861 SQGGFGQPAQIGVGQQALGNVLGSFGQSRQLGPSLPGTGSGSPGGFS-GGFTSVKPVGGG 1920
            +QGGFGQPAQIGVGQQALG VLGSFGQSRQLGPSLPGT SGSPGGF+ GGFTSVKPVGGG
Sbjct: 1861 TQGGFGQPAQIGVGQQALGTVLGSFGQSRQLGPSLPGTASGSPGGFNGGGFTSVKPVGGG 1663

Query: 1921 FAGVGTGGGGGFAGVGSGGGGGFGSVGSGGGGGFGS---GGFAGAASTGGGFAGAASGFG 1980
            FAGVG+GGGGGF G G GGGG  G+  +GGG    S   GGFAGAA  GGGFAGAA GFG
Sbjct: 1921 FAGVGSGGGGGFGGGGFGGGGFAGAASTGGGFAGASPPTGGFAGAA--GGGFAGAAGGFG 1663

Query: 1981 AFGSQQGSSGFSAFAGGGGGAGGTGKPPELFTQIRK 2011
            AFG+QQGS GFSAF    GG+GGTGKPPELFTQIRK
Sbjct: 1981 AFGNQQGSGGFSAFGAAPGGSGGTGKPPELFTQIRK 1663

BLAST of Spg009835 vs. NCBI nr
Match: XP_022966766.1 (nuclear pore complex protein NUP214 isoform X2 [Cucurbita maxima])

HSP 1 Score: 2340.5 bits (6064), Expect = 0.0e+00
Identity = 1374/2021 (67.99%), Postives = 1469/2021 (72.69%), Query Frame = 0

Query: 1    MASVDSRHSTSSTQIPLEDGDEGEHVQTTDYYFEKIGEPVPVKLNDSIIDPESPPSQPLA 60
            MASVDSRHSTSST IPLED  EGEHV+T DYYFEKIGEPVPVKLNDSI DP SPPSQPLA
Sbjct: 1    MASVDSRHSTSSTPIPLEDSYEGEHVETNDYYFEKIGEPVPVKLNDSIFDPGSPPSQPLA 60

Query: 61   VSESFGLIFVAHLSGFFVVRTEDVIASAKEMKNGGAGSSVQDLSIVDVSVGKVHILALSS 120
            VSESFGLIFVAHLSGFF VRT+DV+ASAKEMKNGG GSS+QDLSIVDVSVGKVH+LALS+
Sbjct: 61   VSESFGLIFVAHLSGFFAVRTKDVVASAKEMKNGGTGSSIQDLSIVDVSVGKVHVLALSN 120

Query: 121  DNSILAAVVAGDVHLFSVGSLLDKAEKPSSSHSITDTSCIKDFKWTRKLENSYLVLSKHG 180
            DNS LAAVVAGDVHLF V SLLDK E+PS S S TD+SCIKDFKWTRK ENSYLVLSKHG
Sbjct: 121  DNSFLAAVVAGDVHLFLVDSLLDKGEEPSFSCSTTDSSCIKDFKWTRKPENSYLVLSKHG 180

Query: 181  ELYQGSANGPPKHVMHDIDAVECSVKGKFIAVAKKDTLAIFSYKFKERLSMSLLPSLGNG 240
            +LYQGSA+GP KH+MHDIDAVECSVKGKFIAVAKKDTL +FSYKFKERLSMSLLPSLGNG
Sbjct: 181  KLYQGSASGPFKHIMHDIDAVECSVKGKFIAVAKKDTLTVFSYKFKERLSMSLLPSLGNG 240

Query: 241  NTDTDFAVKGSVSVSPSVSLSKNDICKMLKLEVDCIKWVRADCIIIGCFQVTATGDEEDY 300
            +TDTDFAVK                       VD IKWVRADCIIIGCFQVTATGDEEDY
Sbjct: 241  DTDTDFAVK-----------------------VDSIKWVRADCIIIGCFQVTATGDEEDY 300

Query: 301  FVQVVRSKDGKITDVSSNKVLLSFHDIHSGFTHDILPVETGPCLFLSYLDKCKLAIVANR 360
            FVQV+RSKDGKITDVSSNKVLLSFHDI+SGFT DILPVETGPCL LSYLDKCKLAIVANR
Sbjct: 301  FVQVIRSKDGKITDVSSNKVLLSFHDIYSGFTPDILPVETGPCLLLSYLDKCKLAIVANR 360

Query: 361  NNMDQHIVLLGWLLEVENEVAIIDIERDTSLPRINLQVSDRKTMDVLSLLSLIREVVLGP 420
            NN DQHIVLLGWL EVENEVA+IDIERD SLPRI LQ                       
Sbjct: 361  NNTDQHIVLLGWLQEVENEVAVIDIERDKSLPRIELQ----------------------- 420

Query: 421  GRRDVRLWSPNPIEDFSCRLFFRSLLYPSCENGDDNLVMGLCIDRFSLPGKVKVQVGAEE 480
                                          +NGDDNLVMGLCIDR SLPGKV+VQVG EE
Sbjct: 421  ------------------------------DNGDDNLVMGLCIDRVSLPGKVEVQVGNEE 480

Query: 481  TREVSPYCILLCLTLEGKLIMFHFSSVNESEAPHD-VSACDEEEEDGTVEPSDDQSQLSS 540
             REVSPYC LLCLTLEGKLI+FHFSS NESEA  + VSACDEEEED TV P+DDQ QL  
Sbjct: 481  IREVSPYCTLLCLTLEGKLILFHFSSANESEASDETVSACDEEEEDETVVPTDDQPQLF- 540

Query: 541  ESKNEFREAIVSLKMQDTEKIATNSEIPKEKINISSDIKPSNIDQSSVSNIDESVIVSGE 600
                                                    SNIDQ  VS +D S +++ E
Sbjct: 541  ----------------------------------------SNIDQRPVSKVDGSPVITRE 600

Query: 601  SYTKSQKADSFIYSQSLRSSNLERPNNDTGNFDKPVQKFTGLGSVAFSGQSANVPSQSLK 660
            S  KSQ+ DS  +SQ L+ S LERPNN+ GNF KPV+ FTGLGSVAFSGQS +VPSQ LK
Sbjct: 601  SNAKSQQMDSLAFSQPLKPSTLERPNNEIGNFAKPVKNFTGLGSVAFSGQSVDVPSQPLK 660

Query: 661  SSILERPNNEIGNFDKPVQKFTGLGSVAFSGQSANVPSQSLKSSILERPNNEIGNFDKPV 720
            SSILERPNNEIGNF+KP  KFTGLGSVAFSGQS ++P++SLK S LERPNN+IGNF    
Sbjct: 661  SSILERPNNEIGNFNKPFHKFTGLGSVAFSGQSVDMPNESLKPSFLERPNNQIGNF---- 720

Query: 721  QKFTGFGSVTFSGQSAAMPSQSLKSSILERPNNEIGNCDKPVQKFTGLGSVAFSGQSVDV 780
                                                  DKPVQKFTGLGSVAFS QSVDV
Sbjct: 721  --------------------------------------DKPVQKFTGLGSVAFSEQSVDV 780

Query: 781  PSQPFLNVKESTKRLGSTGLQAASELSSDKPMLFKKVDPVSSVLPLNSLQSSKTENYGPS 840
            PS PFLNVKEST +  S                                           
Sbjct: 781  PSHPFLNVKESTIKHSS------------------------------------------- 840

Query: 841  FGAANAFTGFAGKPFQQKDVPSTLTQSERQVTAGSGKIESLPVIRTSQTSLQDNFSTGKT 900
             GAANAFTGFAGKPFQ KDVPSTLTQS RQV+AG+GKIESLPVI++SQ SLQDNFS GK 
Sbjct: 841  -GAANAFTGFAGKPFQPKDVPSTLTQSGRQVSAGAGKIESLPVIQSSQVSLQDNFSLGKI 900

Query: 901  ANEKQDGSDRNYSNVPLAKPMKEMCEGLDKLLESIEEPGGFLDACTAFQNSSVEALELGL 960
            +N+KQDGS+RNY NVPLAKPM EMCEGLD LLESIEEPGGFLDACT FQ SSVEAL LGL
Sbjct: 901  SNKKQDGSERNYGNVPLAKPMNEMCEGLDMLLESIEEPGGFLDACTTFQKSSVEALGLGL 960

Query: 961  ATLSDQCQIWRRTMNKCAQEVPNLFDKTVQVLQKKTYIEGIVTQASDSNYWEHWDRQKLS 1020
            ATLSDQCQIWRRTM + AQEV NLFD+TV+VL KKTYIEGIVTQASDSNYW+HWDRQKLS
Sbjct: 961  ATLSDQCQIWRRTMTERAQEVQNLFDRTVEVLSKKTYIEGIVTQASDSNYWDHWDRQKLS 1020

Query: 1021 SELELKRQHILKMNQNITNQLIELERHFNGLELNKFGGNDESQVSERALQRKFGSTRHSH 1080
            SELELKRQ IL+MNQN+TNQLIELERHFNGLELN FGGN+E QV+ER LQRKFGS+R SH
Sbjct: 1021 SELELKRQRILQMNQNMTNQLIELERHFNGLELNTFGGNEEIQVNERTLQRKFGSSRQSH 1080

Query: 1081 SLHSLNNIMGSQLAAAQLLSENLSKQMAALNIESPSLKRQSVTKELFETIGITYDASFSS 1140
            SLHSLNNIMGSQLAAAQLLS+NLSKQ+A LNI+SPS KRQS+TKELFETIGITYDASFSS
Sbjct: 1081 SLHSLNNIMGSQLAAAQLLSDNLSKQIATLNIKSPSSKRQSITKELFETIGITYDASFSS 1140

Query: 1141 PNVNKIAETSTSKKLLLSADSFSSKDTSRRKQQSGTKNSEAETGRRRRDSLDRRRETTVG 1200
            PNVNKI ETS SKKLLLSADSFSSKDTSRRKQ+SG K SE ETGRRRRDSLDR       
Sbjct: 1141 PNVNKIPETS-SKKLLLSADSFSSKDTSRRKQRSGAKISETETGRRRRDSLDR------- 1200

Query: 1201 GAVRPDLGSGDGESTVRGGGARAGIRAARFVPLQGLDGGEVSRRQGEEGERRAAEEGERR 1260
                                                                        
Sbjct: 1201 ------------------------------------------------------------ 1260

Query: 1261 AANGRRATSCERAASDERTERARRRRNAEGHIRESHVQNALSYLQELLLYYYGFSVLIRH 1320
                                                                        
Sbjct: 1261 ------------------------------------------------------------ 1320

Query: 1321 VYELEIGFMEMAKNLASVEPPKTTVKRMLLQGIPLSGEKQFRSRTPEGVAAVARPASCIT 1380
                         NLAS++PPKTTVKRM+LQG PLS EKQFRS T EG A VARPA  I 
Sbjct: 1321 -------------NLASIQPPKTTVKRMILQGTPLSNEKQFRSPTLEGPATVARPAGRIP 1380

Query: 1381 SSMLSSSSKNAENGSENPATPFTWASPPQPSNSSRQKSQPLQKANATTPSPLPVFQSSHE 1440
            SSMLSSSSKNAE GSENPATPF+WASPP      RQK QPLQK N T PSPLPV+QSSHE
Sbjct: 1381 SSMLSSSSKNAEQGSENPATPFSWASPP------RQKFQPLQKTNGTAPSPLPVYQSSHE 1440

Query: 1441 MLKKGTNEAYSVTSENKFAEVTFPEKSKSSDFFSLTRNDSVQKPNMNLDQKSSIFTIPAK 1500
            M+KK  +EAYS  SENKFAEVT+PEKSK+SDFFSL R+DSVQK NMN +QKSS F   +K
Sbjct: 1441 MVKKSNSEAYSAASENKFAEVTYPEKSKASDFFSLARSDSVQKSNMNFEQKSSFFVTSSK 1500

Query: 1501 QTPTPKDSIDTSNSNSQKTANVKERHTTTSPLFGSANKPESAFVGTASSLVSTVDGARKT 1560
               TPKDSI+T N NSQKTANVKER TT SPLFG+ANKPE A VGT SSLV TVD  RKT
Sbjct: 1501 PMSTPKDSIETLNPNSQKTANVKERLTTPSPLFGAANKPEPASVGTTSSLVPTVDELRKT 1560

Query: 1561 EEKKSTIAFSPSVPAPALFNTPSSASTLFSGFPVSKSLPSSAAVIDLNKPGSTSTQLIFS 1620
            EEKK    FSPSVPA    NTPSSASTLFSG P+SKS PS AAV+DLNKP STSTQ  F+
Sbjct: 1561 EEKKPPTVFSPSVPASVPVNTPSSASTLFSGSPLSKSFPSPAAVVDLNKPLSTSTQSSFA 1620

Query: 1621 SPVVSVSDSLFQAPKMVSPSPTLSSLNPTLDSSKKELPVPKSDTDTEKQASASKPESHEL 1680
            SPVVSVSDSLFQAPKMVSP  TLSSLNP+L SS KE P+PKSD DTEKQA ASKPE  EL
Sbjct: 1621 SPVVSVSDSLFQAPKMVSPPSTLSSLNPSLVSSSKEQPIPKSDADTEKQAQASKPEFREL 1668

Query: 1681 KLQPPVTPADKNHVEPTSGTQMVSKDVGGHVPNVIGDAQPQQPSAAFVPLSAPNLTSKIS 1740
            KLQP VT A  NHVEPTS TQ VSKDVGGHVP+VI DAQPQQ SAAFVPL +PN T K+S
Sbjct: 1681 KLQPSVTLAVGNHVEPTSVTQTVSKDVGGHVPSVIADAQPQQSSAAFVPLPSPNSTPKVS 1668

Query: 1741 ANGKNETSDAVVTQDDDMDEEAPETNNNVEFSLSALGGFG-SSSPISSAPKPNPFGGPFG 1800
            ANGK+ETSDA++TQDDDMDEEAPET NNVEFSLS+LGGFG +S+P+S+APKPNPFGG FG
Sbjct: 1741 ANGKSETSDALITQDDDMDEEAPET-NNVEFSLSSLGGFGTTSTPMSNAPKPNPFGGSFG 1668

Query: 1801 NVNATSMNSSFTMAPPQSGELFRPASFSFQSPLASQAASQPTNSVAFSGGFGSGMATQAP 1860
            N NATSMNSSFT A P SGELFRPASFSFQSPLASQAASQPTNSVAFS  FGSGMATQAP
Sbjct: 1801 NANATSMNSSFTTASPPSGELFRPASFSFQSPLASQAASQPTNSVAFSSSFGSGMATQAP 1668

Query: 1861 SQGGFGQPAQIGVGQQALGNVLGSFGQSRQLGPSLPGTGSGSPGGFS-GGFTSVKPVGGG 1920
            +QGGFGQPAQIGVGQQALG VLGSFGQSRQLGPSLPGT SGSPGGF+ GGFTSVKPVGGG
Sbjct: 1861 TQGGFGQPAQIGVGQQALGTVLGSFGQSRQLGPSLPGTASGSPGGFNGGGFTSVKPVGGG 1668

Query: 1921 FAGVGTGGGGGFAGVGSG----GGGGFGSVGSGGGGGFG----SGGFAGAASTGGGFAGA 1980
            FAGVG+GGGGGF G G G    GGGGF    S GGG  G    +GGFAGAA  GGGFAGA
Sbjct: 1921 FAGVGSGGGGGFGGGGFGGGGFGGGGFAGAASTGGGFAGASPPTGGFAGAA--GGGFAGA 1668

Query: 1981 ASGFGAFGSQQGSSGFSAFAGGGGGAGGTGKPPELFTQIRK 2011
            A GFGAFG+QQGS GFSAF    GG+GGTGKPPELFTQIRK
Sbjct: 1981 AGGFGAFGNQQGSGGFSAFGAAPGGSGGTGKPPELFTQIRK 1668

BLAST of Spg009835 vs. ExPASy Swiss-Prot
Match: F4I1T7 (Nuclear pore complex protein NUP214 OS=Arabidopsis thaliana OX=3702 GN=NUP214 PE=1 SV=1)

HSP 1 Score: 773.5 bits (1996), Expect = 6.0e-222
Identity = 737/2123 (34.72%), Postives = 1019/2123 (48.00%), Query Frame = 0

Query: 13   TQIPLEDGDEGEHVQTTDYYFEKIGEPVPVKLNDSIIDPESPPSQPLAVSESFGLIFVAH 72
            +++ +E+  EG+ + T DYYFE+IGEP+ +K +D+  D E+PPSQPLA+SE   ++FVAH
Sbjct: 2    SRVEIEEDTEGDRISTNDYYFERIGEPISIKEDDAQYDLENPPSQPLAISERHAVLFVAH 61

Query: 73   LSGFFVVRTEDVIASAKEMKNGGAGSSVQDLSIVDVSVGKVHILALSSDNSILAAVVAGD 132
             SGFFV RT DVI+++K     G    +QDLS+VDV VG V IL+LS+D+SILA  VA D
Sbjct: 62   SSGFFVGRTNDVISASKNSNGNGDKVFIQDLSLVDVPVGDVRILSLSADDSILAVTVAAD 121

Query: 133  VHLFSVGSLLDKAEKPSSSHSITDTSCIKDFKWTRKLENSYLVLSKHGELYQGSANGPPK 192
            +H FSV SLL K  KPS S+S  ++  +KDF+W R  ++SYLVLS  G+L+ G  N PP+
Sbjct: 122  IHFFSVDSLLKKDAKPSFSYSPDESGFVKDFRWRRNDKHSYLVLSNTGKLFHGIDNAPPR 181

Query: 193  HVMHDIDAVECSVKGKFIAVAKKDTLAIFSYKFKERLSMSLLPSLGNGNTDTDFAVKGSV 252
            HVM  +DAVE S KG +IAVA+ ++L IFS KF E+  ++L      G++D D  VK   
Sbjct: 182  HVMDAVDAVEWSSKGSYIAVAQDNSLRIFSSKFNEKRCIALSFDSWIGDSDEDCFVK--- 241

Query: 253  SVSPSVSLSKNDICKMLKLEVDCIKWVRADCIIIGCFQVTATGDEEDYFVQVVRSKDGKI 312
                                VD I+WVR +CI++GCFQ+   G EE+Y VQV+RS DGKI
Sbjct: 242  --------------------VDSIRWVRNNCILLGCFQL-IEGREENYLVQVIRSPDGKI 301

Query: 313  TDVSSNKVLLSFHDIHSGFTHDILPVETGPCLFLSYLDKCKLAIVANRNNMDQHIVLLGW 372
            +D S+N V LSF D+      D++PV  GP L  SY+D+CKLA+ ANR ++D+HIVLL W
Sbjct: 302  SDGSTNLVALSFSDLFPCSMDDLVPVGVGPHLLFSYIDQCKLAVTANRKSIDEHIVLLDW 361

Query: 373  LL-EVENEVAIIDIERDTSLPRINLQVSDRKTMDVLSLLSLIREVVLGPGRRDVRLWSPN 432
               + ++ V+++DI+R+T LPRI LQ                                  
Sbjct: 362  SSGDDKSAVSVVDIDRETFLPRIGLQ---------------------------------- 421

Query: 433  PIEDFSCRLFFRSLLYPSCENGDDNLVMGLCIDRFSLPGKVKVQVGAEETREVSPYCILL 492
                               EN DDN VMGLCIDR S+ G V V+ G +E +E+ PY +L+
Sbjct: 422  -------------------ENNDDNTVMGLCIDRVSIEGTVNVRSGDDELKELQPYFVLV 481

Query: 493  CLTLEGKLIMFHFSSVNESEAPHDVSACDEEEEDGTVEP--SDDQSQLSSESKNEFREAI 552
            CLTLEGKL+MF+ +SV    A  D       + +    P   DD S+ SSE   +   A+
Sbjct: 482  CLTLEGKLVMFNVASVAGRPASSDTDLASSSDIEDAYTPLIEDDLSKQSSEKHQQLNIAV 541

Query: 553  VS-LKMQDTEKIATNSEIPKEKINISSDIKPSNIDQSSVSNIDESVIVSGESYTKSQK-A 612
             +  K  +TEK +T   +P E           NI      ++  S  VSG++  K +  A
Sbjct: 542  QNDQKHLNTEKFSTEQRLPNE-----------NIFSKEFESVKSS--VSGDNNKKQEPYA 601

Query: 613  DSFIYSQSLRSSNLERPNNDTGNFDKPVQKFTGLGSVAFSGQSANVPSQSLKSSILERPN 672
            +  +  +  + S + R                       SG S      SL         
Sbjct: 602  EKPLQVEDAQQSMIPR----------------------LSGTSFGQLPMSL--------- 661

Query: 673  NEIGNFDKPVQKFTGLGSVAFSGQSANVPSQSLKSSILERPNNEIGNFDKPVQKFTGFGS 732
                 +D    KF G G        A   S+ L+  I  + N+        +Q      +
Sbjct: 662  ----GYD--TNKFAGFG-------PALPVSEKLQKDIFAQSNS------MHLQ-----AN 721

Query: 733  VTFSGQSAAMPSQSLKSSILERPNNEIGNCDKPVQKFTGLGSVAFSGQSVDVP---SQPF 792
            V     +A   S  L+++IL+ P N      +P            SG+SV  P   S PF
Sbjct: 722  VESKSTAAFFGSPGLQNAILQSPQN---TSSQPWS----------SGKSVSPPDFVSGPF 781

Query: 793  LNVKESTKRLGSTGLQAASELSSDKPMLFKKVDPVSSVLPLNSLQSSKTENYGPSFGAAN 852
             +++++  +     +Q+ +    + PM  K      SV  + + + S   N  P  G   
Sbjct: 782  PSMRDTQHK---QSVQSGTGY-VNPPMSIKD----KSVQVIETGRVSALSNLSPLLG--- 841

Query: 853  AFTGFAGKPFQQKDVPSTLTQSERQVTAGSGKIESLPVIRTSQTSLQDNFSTGKTANEKQ 912
                                   +    G  KIE +P IR SQ S Q   S  K+A+ +Q
Sbjct: 842  ---------------------QNQDTNEGVEKIEPIPSIRASQLSQQVKSSFEKSASHQQ 901

Query: 913  DGS---------DRNYSNVPLAKPMKEMCEGLDKLLESIEEPGGFLDACTAFQNSSVEAL 972
              +         + N SN P    + EM   +D LL+SIE PGGF D+C     S+VE L
Sbjct: 902  HKTPLSTGPLRLEHNMSNQP--SNINEMAREMDTLLQSIEGPGGFKDSCAFILKSNVEEL 961

Query: 973  ELGLATLSDQCQIWRRTMNKCAQEVPNLFDKTVQVLQKKTYIEGIVTQASDSNYWEHWDR 1032
            E GL +L+ +CQ W+ T+++   E+ +L DKT+QVL KKTY+EG+  Q +D+ YW+ W+R
Sbjct: 962  EQGLESLAGKCQTWKSTIHEQQAEIQHLLDKTIQVLAKKTYMEGMYKQTADNQYWQLWNR 1021

Query: 1033 QKLSSELELKRQHILKMNQNITNQLIELERHFNGLELNKFGGNDESQVSERALQRKFGST 1092
            QKL+ ELE KRQHI+K+N+++T+QLIELER+FN LEL+++  +    V+ R +  +   +
Sbjct: 1022 QKLNPELEAKRQHIMKLNKDLTHQLIELERYFNRLELDRYNEDGGHPVARRGVPNRSAPS 1081

Query: 1093 RHSHSLHSLNNIMGSQLAAAQLLSENLSKQMAALNIESPSLKRQSVTKELFETIGITYDA 1152
            R   SLHSL+N M SQLAAA+ LSE LSKQM  L I+SP   +++V +ELFETIGI YDA
Sbjct: 1082 RRVQSLHSLHNTMSSQLAAAEQLSECLSKQMTYLKIDSP--VKKNVKQELFETIGIPYDA 1141

Query: 1153 SFSSPNVNKIAETSTSKKLLLSADSFSSKDTSRRKQQSGTKNSEAETGRRRRDSLDRRRE 1212
            SFSSP+  K    S++K LLLS+   S    SR++Q S  KNS+ ET RRRR+SLD    
Sbjct: 1142 SFSSPDAVKAKNASSAKNLLLSSIPASINQQSRQRQSSAMKNSDPETARRRRESLD---- 1201

Query: 1213 TTVGGAVRPDLGSGDGESTVRGGGARAGIRAARFVPLQGLDGGEVSRRQGEEGERRAAEE 1272
                                     R     A F P +               +R   +E
Sbjct: 1202 -------------------------RVIFNWAAFEPPK------------TTVKRMLLQE 1261

Query: 1273 GERRAANGRRATSCERAASDERTERARRRRNAEGHIRESHVQNALSYLQELLLYYYGFSV 1332
             ++   N +   S          ER R   N +      HV++  S              
Sbjct: 1262 QQKTGMNQQTVLS----------ERLRSANNTQDR-SLLHVKDHAS-------------- 1321

Query: 1333 LIRHVYELEIGFMEMAKNLASVEPPKTTVKRMLLQGIPLSGEKQFRSRTPEGVAAVARPA 1392
                V     G ME  +   S             Q  P      F++R P     + +  
Sbjct: 1322 ---PVVSSNKGIMESFQQDTSE-----------AQSTP------FKTRPP-----MPQSN 1381

Query: 1393 SCITSSMLSSS--SKNAENGSENPATPFTWASPPQPSNSSRQKSQPLQKANATTPSPLPV 1452
            S  T S +S+S  S N      +  T +   S P     +R  SQP     ++     PV
Sbjct: 1382 SPFTISPISASKPSFNWSGNKSSNTTSYAEESAPSQIKDTRTVSQP---GGSSFLPKRPV 1441

Query: 1453 FQSSHEMLKKGTNE-AYSVTSENKFAEVT------FPEKSKSSDFFS------------- 1512
              +  E  +K   E  +S    N F E            S  SDF S             
Sbjct: 1442 ASTVLEQTEKKAGEFKFSEAKANAFVETAAGSVQRLSTTSSGSDFESSKGFGAQFSTMSS 1501

Query: 1513 -LTRNDSVQKPNMNLDQKSSI----FTIPAKQTP---TPKDSIDT-SNSNSQKTANVKER 1572
                +    K     +  SSI    FT PA   P   TP DS  T   ++S   ++  + 
Sbjct: 1502 GAPASSFSSKSLFGFNSSSSIPGDKFTFPAVTAPLSGTPLDSTSTLFTASSAPVSSSSQD 1561

Query: 1573 HTTTSPLFGSANKPESAFVGTASSLVSTVD-----GARKTEEKKSTIAFSPSVPAPA--- 1632
                S    SA  P++ F  T++S VS        G   T  K      +PS P+P+   
Sbjct: 1562 PVPASIPISSAPVPQT-FSVTSTSTVSATGFNVPFGKPLTSVKVDLNQAAPSTPSPSPGP 1621

Query: 1633 ----LFN----TPSSASTLFSGFPVSKSLPSSAAVIDLNKPGSTSTQLIFSSPVVSVSDS 1692
                 FN    +PSS   + S    S   P SA    ++   +++T  +  S  +  S S
Sbjct: 1622 TAGFTFNLPALSPSSPEMVSSSTGQSSLFPPSAPTSQVSSDQASATSSLTDSSRLFSSTS 1681

Query: 1693 L-----------FQAPKMVSPSPTLSSLNPTLDSSKKEL---PVPKSDTDTEKQASASKP 1752
            L           FQ+P++ +PS  +    P  +  K E     +  + +  +  A+A+K 
Sbjct: 1682 LSSTPPITPPDAFQSPQVSTPSSAVPITEPVSEPKKPEAQSSSILSTQSTVDSVANATKT 1741

Query: 1753 ESHELKLQ-------PPVTPADKNHVEP--TSGTQMVSKDVGGHVPNVIGDAQPQQPSAA 1812
            ++  L ++         VTP   +      +SGTQ     +     +  G +QPQQ S+ 
Sbjct: 1742 QNEPLPVKSEISNPGTTVTPVSSSGFLSGFSSGTQSSLASMAAPSFSWPGSSQPQQLSST 1801

Query: 1813 FVPLSAPNLTSKISANGKNETSDAVVTQDDDMDEEAPETNNNVEFSLSALGGFG-SSSPI 1872
              P  A   +S  SA+   E  D V TQ+D+MDEEAPE +   E S+ + GGFG  S+P 
Sbjct: 1802 PAPFPA---SSPTSASPFGEKKDIVDTQEDEMDEEAPEASQTTELSMGSFGGFGLGSTPN 1819

Query: 1873 SSAPKPNPFGGPFGNVNATSMNSSFTMAPPQSGELFRPASFSFQSPLASQAASQPTNSVA 1932
              APK NPFGGPFGN   T+ N  F M  P SGELF+PASF+FQ+P  SQ A        
Sbjct: 1862 PGAPKTNPFGGPFGNATTTTSN-PFNMTVP-SGELFKPASFNFQNPQPSQPA-------- 1819

Query: 1933 FSGGFGSGMAT--QAPSQGGFGQPAQIGVGQQALGNVLGSFGQSRQLGPSLPGTGSGSPG 1992
               GFGS   T  Q P+Q GFGQP+QIG GQQALG+VLGSFGQSRQ+G  LPG   GSP 
Sbjct: 1922 ---GFGSFSVTPSQTPAQSGFGQPSQIGGGQQALGSVLGSFGQSRQIGAGLPGATFGSPT 1819

Query: 1993 GF-------------------------SGGFTSVKPVGGGFAGVGTGGGGGFAGVGSGGG 2011
            GF                         +GGF ++   G GFAG  +   GGFA + SG G
Sbjct: 1982 GFGGSNPGSGLPNAPASGGFAAAGSSATGGFAAMASAGRGFAGASSTPTGGFAALASGSG 1819

BLAST of Spg009835 vs. ExPASy TrEMBL
Match: A0A6J1G089 (nuclear pore complex protein NUP214 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111449495 PE=4 SV=1)

HSP 1 Score: 2343.2 bits (6071), Expect = 0.0e+00
Identity = 1379/2016 (68.40%), Postives = 1470/2016 (72.92%), Query Frame = 0

Query: 1    MASVDSRHSTSSTQIPLEDGDEGEHVQTTDYYFEKIGEPVPVKLNDSIIDPESPPSQPLA 60
            MASVDSRHS S T I LED  EGEHV+T DYYFEKIGEPVPVKLNDSI DP SPPSQPLA
Sbjct: 1    MASVDSRHSISLTPIALEDSYEGEHVETNDYYFEKIGEPVPVKLNDSIFDPGSPPSQPLA 60

Query: 61   VSESFGLIFVAHLSGFFVVRTEDVIASAKEMKNGGAGSSVQDLSIVDVSVGKVHILALSS 120
            VSESFGLIFVAHLSGFFVVRT DV+ASAKEMKNGG GSS+QDLSIVDVSVGKVH+LALS+
Sbjct: 61   VSESFGLIFVAHLSGFFVVRTTDVVASAKEMKNGGTGSSIQDLSIVDVSVGKVHVLALSN 120

Query: 121  DNSILAAVVAGDVHLFSVGSLLDKAEKPSSSHSITDTSCIKDFKWTRKLENSYLVLSKHG 180
            DNS LAAVVAGDVHLFSV SLLDKAEKP  S S TD+SCIKDFKWTRK ENSYLVLSKHG
Sbjct: 121  DNSFLAAVVAGDVHLFSVDSLLDKAEKPYFSCSTTDSSCIKDFKWTRKPENSYLVLSKHG 180

Query: 181  ELYQGSANGPPKHVMHDIDAVECSVKGKFIAVAKKDTLAIFSYKFKERLSMSLLPSLGNG 240
            +LYQGSA+ P KH+MHDIDAVECSVKGKFIAVAKKDTL+IFSYKFKERLSMSLLPS GNG
Sbjct: 181  KLYQGSASVPFKHIMHDIDAVECSVKGKFIAVAKKDTLSIFSYKFKERLSMSLLPSSGNG 240

Query: 241  NTDTDFAVKGSVSVSPSVSLSKNDICKMLKLEVDCIKWVRADCIIIGCFQVTATGDEEDY 300
            +TDTDFA+K                       VD IKWVRADCIIIGCFQVTATGDEEDY
Sbjct: 241  DTDTDFAMK-----------------------VDSIKWVRADCIIIGCFQVTATGDEEDY 300

Query: 301  FVQVVRSKDGKITDVSSNKVLLSFHDIHSGFTHDILPVETGPCLFLSYLDKCKLAIVANR 360
            FVQV+RSKDGKITDVSSNKVLLSFHDI+SGFT DILPVETGPCL LSYLDKCKLAIVANR
Sbjct: 301  FVQVIRSKDGKITDVSSNKVLLSFHDIYSGFTPDILPVETGPCLLLSYLDKCKLAIVANR 360

Query: 361  NNMDQHIVLLGWLLEVENEVAIIDIERDTSLPRINLQVSDRKTMDVLSLLSLIREVVLGP 420
            NN DQHIVLLGWL EVENEVA+IDIERD SLPRI LQ                       
Sbjct: 361  NNTDQHIVLLGWLQEVENEVAVIDIERDKSLPRIELQ----------------------- 420

Query: 421  GRRDVRLWSPNPIEDFSCRLFFRSLLYPSCENGDDNLVMGLCIDRFSLPGKVKVQVGAEE 480
                                          +NGDDNLVMGLCIDR SLPGKV+VQVG EE
Sbjct: 421  ------------------------------DNGDDNLVMGLCIDRVSLPGKVEVQVGNEE 480

Query: 481  TREVSPYCILLCLTLEGKLIMFHFSSVNESEAPHD-VSACDEEEEDGTVEPSDDQSQLSS 540
             REVSPYC LLCLTLEGKL++FHFSS NESEA  + VSACDEEEED TV P+DDQ QL  
Sbjct: 481  IREVSPYCTLLCLTLEGKLLLFHFSSANESEASDETVSACDEEEEDDTVVPTDDQPQLF- 540

Query: 541  ESKNEFREAIVSLKMQDTEKIATNSEIPKEKINISSDIKPSNIDQSSVSNIDESVIVSGE 600
                                                    SNIDQ  VS +DES +++ E
Sbjct: 541  ----------------------------------------SNIDQRPVSEVDESPVITRE 600

Query: 601  SYTKSQKADSFIYSQSLRSSNLERPNNDTGNFDKPVQKFTGLGSVAFSGQSANVPSQSLK 660
            S  KSQ+ DSF +SQ L+ S LERPNN+ GNF KPV+ FTGLGSVAFSGQS +VPSQSLK
Sbjct: 601  SNAKSQQMDSFAFSQPLKPSTLERPNNEIGNFTKPVKSFTGLGSVAFSGQSVDVPSQSLK 660

Query: 661  SSILERPNNEIGNFDKPVQKFTGLGSVAFSGQSANVPSQSLKSSILERPNNEIGNFDKPV 720
            SSILERPNNEIGNF+KP  KFTGLGSVAFSGQS ++ +QSLK S LERPNN+IGNF    
Sbjct: 661  SSILERPNNEIGNFNKPFHKFTGLGSVAFSGQSVDMANQSLKPSFLERPNNQIGNF---- 720

Query: 721  QKFTGFGSVTFSGQSAAMPSQSLKSSILERPNNEIGNCDKPVQKFTGLGSVAFSGQSVDV 780
                                                  DKPVQKFTGLGSVAFS QS DV
Sbjct: 721  --------------------------------------DKPVQKFTGLGSVAFSEQSADV 780

Query: 781  PSQPFLNVKESTKRLGSTGLQAASELSSDKPMLFKKVDPVSSVLPLNSLQSSKTENYGPS 840
            PS PFLNVKEST +                                   QSS        
Sbjct: 781  PSHPFLNVKESTIK-----------------------------------QSS-------- 840

Query: 841  FGAANAFTGFAGKPFQQKDVPSTLTQSERQVTAGSGKIESLPVIRTSQTSLQDNFSTGKT 900
             GAANAFTGFAGKPFQ KDVPSTLTQS RQV+AG+GKIESLPVI++SQ SLQDNFS GK 
Sbjct: 841  -GAANAFTGFAGKPFQPKDVPSTLTQSGRQVSAGAGKIESLPVIQSSQVSLQDNFSLGKI 900

Query: 901  ANEKQDGSDRNYSNVPLAKPMKEMCEGLDKLLESIEEPGGFLDACTAFQNSSVEALELGL 960
            +N+KQDGS+RNY NVPLAKPM EMCEGLD LLESIEEPGGFLDACT FQ SSVEALELGL
Sbjct: 901  SNKKQDGSERNYGNVPLAKPMTEMCEGLDMLLESIEEPGGFLDACTTFQKSSVEALELGL 960

Query: 961  ATLSDQCQIWRRTMNKCAQEVPNLFDKTVQVLQKKTYIEGIVTQASDSNYWEHWDRQKLS 1020
            ATLSDQCQIWRRTM + AQEV NLFD+TV+VL KKTYIEGIVTQASDSNYWEHWDRQKLS
Sbjct: 961  ATLSDQCQIWRRTMTERAQEVQNLFDRTVEVLSKKTYIEGIVTQASDSNYWEHWDRQKLS 1020

Query: 1021 SELELKRQHILKMNQNITNQLIELERHFNGLELNKFGGNDESQVSERALQRKFGSTRHSH 1080
            SELELKRQ IL+MNQN+TNQLIELERHFNGLELNKFGGN+E QV+ER LQRKFGS+R SH
Sbjct: 1021 SELELKRQRILQMNQNMTNQLIELERHFNGLELNKFGGNEEIQVNERTLQRKFGSSRQSH 1080

Query: 1081 SLHSLNNIMGSQLAAAQLLSENLSKQMAALNIESPSLKRQSVTKELFETIGITYDASFSS 1140
            SLHSLNNIMGSQLAAAQLLS+NLSKQ+A LNIESPS KRQS+TKELFETIGITYDASFSS
Sbjct: 1081 SLHSLNNIMGSQLAAAQLLSDNLSKQIATLNIESPSSKRQSITKELFETIGITYDASFSS 1140

Query: 1141 PNVNKIAETSTSKKLLLSADSFSSKDTSRRKQQSGTKNSEAETGRRRRDSLDRRRETTVG 1200
            PNVNKI ETS SKKLLLSADSFSSKDTSRRKQ+SG K SE ETGRRRRDSLDR       
Sbjct: 1141 PNVNKIPETS-SKKLLLSADSFSSKDTSRRKQRSGAKISETETGRRRRDSLDR------- 1200

Query: 1201 GAVRPDLGSGDGESTVRGGGARAGIRAARFVPLQGLDGGEVSRRQGEEGERRAAEEGERR 1260
                                                                        
Sbjct: 1201 ------------------------------------------------------------ 1260

Query: 1261 AANGRRATSCERAASDERTERARRRRNAEGHIRESHVQNALSYLQELLLYYYGFSVLIRH 1320
                                                                        
Sbjct: 1261 ------------------------------------------------------------ 1320

Query: 1321 VYELEIGFMEMAKNLASVEPPKTTVKRMLLQGIPLSGEKQFRSRTPEGVAAVARPASCIT 1380
                         NLASV+PPKTTVKRM+LQG PLS EKQFRS T EG A +ARPAS I 
Sbjct: 1321 -------------NLASVQPPKTTVKRMILQGTPLSNEKQFRSPTLEGPATIARPASRIP 1380

Query: 1381 SSMLSSSSKNAENGSENPATPFTWASPPQPSNSSRQKSQPLQKANATTPSPLPVFQSSHE 1440
            SSMLSSSSKNAE GS+NPATPF+WASPP      RQK QPLQK N T PS LPVFQSSHE
Sbjct: 1381 SSMLSSSSKNAEQGSKNPATPFSWASPP------RQKFQPLQKTNGTAPSSLPVFQSSHE 1440

Query: 1441 MLKKGTNEAYSVTSENKFAEVTFPEKSKSSDFFSLTRNDSVQKPNMNLDQKSSIFTIPAK 1500
            M+KK  +EAYS  SENKFAEVT+PEKSK+SDFFSL R+DSVQK NMN +QKSSIF   +K
Sbjct: 1441 MVKKSNSEAYSAASENKFAEVTYPEKSKASDFFSLARSDSVQKSNMNFEQKSSIFVTSSK 1500

Query: 1501 QTPTPKDSIDTSNSNSQKTANVKERHTTTSPLFGSANKPESAFVGTASSLVSTVDGARKT 1560
               TPKDSI+T N NSQKTANVKER TT SPLFGSANKPE   VGT SSLV TVDG RKT
Sbjct: 1501 PMSTPKDSIETLNPNSQKTANVKERLTTPSPLFGSANKPEPVSVGTTSSLVPTVDGLRKT 1560

Query: 1561 EEKKSTIAFSPSVPAPALFNTPSSASTLFSGFPVSKSLPSSAAVIDLNKPGSTSTQLIFS 1620
            EEKK    FSPSVPAPA  NTP SASTLFSG P+SKS PS AAV+DLNKP STSTQ  F+
Sbjct: 1561 EEKKPPTVFSPSVPAPAPVNTPPSASTLFSGSPLSKSFPSPAAVVDLNKPLSTSTQSSFA 1620

Query: 1621 SPVVSVSDSLFQAPKMVSPSPTLSSLNPTLDSSKKELPVPKSDTDTEKQASASKPESHEL 1680
            SPVVSVSDSLFQAPKMVSP   LSSLNPTL SS KE P+PKSD DTEKQA ASKPES EL
Sbjct: 1621 SPVVSVSDSLFQAPKMVSPPSNLSSLNPTLVSSSKEQPMPKSDADTEKQAPASKPESREL 1663

Query: 1681 KLQPPVTPADKNHVEPTSGTQMVSKDVGGHVPNVIGDAQPQQPSAAFVPLSAPNLTSKIS 1740
            KLQP VT A  NHVEPTS TQ VSKDVGGHVP V+ DAQPQQ  AAFVPL  PN TSK +
Sbjct: 1681 KLQPSVTLAVGNHVEPTSVTQTVSKDVGGHVPFVVADAQPQQSPAAFVPLPTPNSTSKAA 1663

Query: 1741 ANGKNETSDAVVTQDDDMDEEAPETNNNVEFSLSALGGFG-SSSPISSAPKPNPFGGPFG 1800
            ANGK+ETSDA++TQDDDMDEEAPET NNVEFSLS+LGGFG +S+P+S+APKPNPFGG FG
Sbjct: 1741 ANGKSETSDALITQDDDMDEEAPET-NNVEFSLSSLGGFGTTSTPMSNAPKPNPFGGSFG 1663

Query: 1801 NVNATSMNSSFTMAPPQSGELFRPASFSFQSPLASQAASQPTNSVAFSGGFGSGMATQAP 1860
            NVNATSMNSSFT A P SGELFRPASFSFQSPLASQAASQPTN+VAFSG FGSGMATQAP
Sbjct: 1801 NVNATSMNSSFTTASPPSGELFRPASFSFQSPLASQAASQPTNAVAFSGSFGSGMATQAP 1663

Query: 1861 SQGGFGQPAQIGVGQQALGNVLGSFGQSRQLGPSLPGTGSGSPGGFS-GGFTSVKPVGGG 1920
            +QGGFGQPAQIGVGQQALG VLGSFGQSRQLGPSLPGT SGSPGGF+ GGFTSVKPVGGG
Sbjct: 1861 AQGGFGQPAQIGVGQQALGTVLGSFGQSRQLGPSLPGTASGSPGGFNGGGFTSVKPVGGG 1663

Query: 1921 FAGVGTGGGGGFAGVGSGGGGGFGSVGSGGGGGFGS---GGFAGAASTGGGFAGAASGFG 1980
            FAGVG+GGGGGF G G  GGG  G+  +GGG    S   GGFAGAA  GGGFAGAA GFG
Sbjct: 1921 FAGVGSGGGGGFGGGGFSGGGFAGAASTGGGFAGASPPTGGFAGAA--GGGFAGAAGGFG 1663

Query: 1981 AFGSQQGSSGFSAFAGGGGGAGGTGKPPELFTQIRK 2011
            AFG+QQGS GFSAF    GG+GGTGKPPELFTQIRK
Sbjct: 1981 AFGNQQGSGGFSAFGAAPGGSGGTGKPPELFTQIRK 1663

BLAST of Spg009835 vs. ExPASy TrEMBL
Match: A0A6J1G030 (nuclear pore complex protein NUP214 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111449495 PE=4 SV=1)

HSP 1 Score: 2342.0 bits (6068), Expect = 0.0e+00
Identity = 1381/2030 (68.03%), Postives = 1473/2030 (72.56%), Query Frame = 0

Query: 1    MASVDSRHSTSSTQIPLEDGDEGEHVQTTDYYFEKIGEPVPVKLNDSIIDPESPPSQPLA 60
            MASVDSRHS S T I LED  EGEHV+T DYYFEKIGEPVPVKLNDSI DP SPPSQPLA
Sbjct: 1    MASVDSRHSISLTPIALEDSYEGEHVETNDYYFEKIGEPVPVKLNDSIFDPGSPPSQPLA 60

Query: 61   VSESFGLIFVAHLSGFFVVRTEDVIASAKEMKNGGAGSSVQDLSIVDVSVGKVHILALSS 120
            VSESFGLIFVAHLSGFFVVRT DV+ASAKEMKNGG GSS+QDLSIVDVSVGKVH+LALS+
Sbjct: 61   VSESFGLIFVAHLSGFFVVRTTDVVASAKEMKNGGTGSSIQDLSIVDVSVGKVHVLALSN 120

Query: 121  DNSILAAVVAGDVHLFSVGSLLDKAEKPSSSHSITDTSCIKDFKWTRKLENSYLVLSKHG 180
            DNS LAAVVAGDVHLFSV SLLDKAEKP  S S TD+SCIKDFKWTRK ENSYLVLSKHG
Sbjct: 121  DNSFLAAVVAGDVHLFSVDSLLDKAEKPYFSCSTTDSSCIKDFKWTRKPENSYLVLSKHG 180

Query: 181  ELYQGSANGPPKHVMHDIDAVECSVKGKFIAVAKKDTLAIFSYKFKERLSMSLLPSLGNG 240
            +LYQGSA+ P KH+MHDIDAVECSVKGKFIAVAKKDTL+IFSYKFKERLSMSLLPS GNG
Sbjct: 181  KLYQGSASVPFKHIMHDIDAVECSVKGKFIAVAKKDTLSIFSYKFKERLSMSLLPSSGNG 240

Query: 241  NTDTDFAVKGSVSVSPSVSLSKNDICKMLKLEVDCIKWVRADCIIIGCFQVTATGDEEDY 300
            +TDTDFA+K                       VD IKWVRADCIIIGCFQVTATGDEEDY
Sbjct: 241  DTDTDFAMK-----------------------VDSIKWVRADCIIIGCFQVTATGDEEDY 300

Query: 301  FVQVVRSKDGKITDVSSNKVLLSFHDIHSGFTHDILPVETGPCLFLSYLDKCKLAIVANR 360
            FVQV+RSKDGKITDVSSNKVLLSFHDI+SGFT DILPVETGPCL LSYLDKCKLAIVANR
Sbjct: 301  FVQVIRSKDGKITDVSSNKVLLSFHDIYSGFTPDILPVETGPCLLLSYLDKCKLAIVANR 360

Query: 361  NNMDQHIVLLGWLLEVENEVAIIDIERDTSLPRINLQVSDRKTMDVLSLLSLIREVVLGP 420
            NN DQHIVLLGWL EVENEVA+IDIERD SLPRI LQ                       
Sbjct: 361  NNTDQHIVLLGWLQEVENEVAVIDIERDKSLPRIELQ----------------------- 420

Query: 421  GRRDVRLWSPNPIEDFSCRLFFRSLLYPSCENGDDNLVMGLCIDRFSLPGKVKVQVGAEE 480
                                          +NGDDNLVMGLCIDR SLPGKV+VQVG EE
Sbjct: 421  ------------------------------DNGDDNLVMGLCIDRVSLPGKVEVQVGNEE 480

Query: 481  TREVSPYCILLCLTLEGKLIMFHFSSVNESEAPHD-VSACDEEEEDGTVEPSDDQSQLSS 540
             REVSPYC LLCLTLEGKL++FHFSS NESEA  + VSACDEEEED TV P+DDQ QL  
Sbjct: 481  IREVSPYCTLLCLTLEGKLLLFHFSSANESEASDETVSACDEEEEDDTVVPTDDQPQLF- 540

Query: 541  ESKNEFREAIVSLKMQDTEKIATNSEIPKEKINISSDIKPSNIDQSSVSNIDESVIVSGE 600
                                                    SNIDQ  VS +DES +++ E
Sbjct: 541  ----------------------------------------SNIDQRPVSEVDESPVITRE 600

Query: 601  SYTKSQKADSFIYSQSLRSSNLERPNNDTGNFDKPVQKFTGLGSVAFSGQSANVPSQSLK 660
            S  KSQ+ DSF +SQ L+ S LERPNN+ GNF KPV+ FTGLGSVAFSGQS +VPSQSLK
Sbjct: 601  SNAKSQQMDSFAFSQPLKPSTLERPNNEIGNFTKPVKSFTGLGSVAFSGQSVDVPSQSLK 660

Query: 661  SSILERPNNEIGNFDKPVQKFTGLGSVAFSGQSANVPSQSLKSSILERPNNEIGNFDKPV 720
            SSILERPNNEIGNF+KP  KFTGLGSVAFSGQS ++ +QSLK S LERPNN+IGNF    
Sbjct: 661  SSILERPNNEIGNFNKPFHKFTGLGSVAFSGQSVDMANQSLKPSFLERPNNQIGNF---- 720

Query: 721  QKFTGFGSVTFSGQSAAMPSQSLKSSILERPNNEIGNCDKPVQKFTGLGSVAFSGQSVDV 780
                                                  DKPVQKFTGLGSVAFS QS DV
Sbjct: 721  --------------------------------------DKPVQKFTGLGSVAFSEQSADV 780

Query: 781  PSQPFLNVKESTKRLGSTGLQAASELSSDKPMLFKKVDPVSSVLPLNSLQSSKTENYGPS 840
            PS PFLNVKEST +                                   QSS        
Sbjct: 781  PSHPFLNVKESTIK-----------------------------------QSS-------- 840

Query: 841  FGAANAFTGFAGKPFQQKDVPSTLTQSERQVTAGSGKIESLPVIRTSQTSLQDNFSTGKT 900
             GAANAFTGFAGKPFQ KDVPSTLTQS RQV+AG+GKIESLPVI++SQ SLQDNFS GK 
Sbjct: 841  -GAANAFTGFAGKPFQPKDVPSTLTQSGRQVSAGAGKIESLPVIQSSQVSLQDNFSLGKI 900

Query: 901  ANEKQDGSDRNYSNVPLAKPMKEMCEGLDKLLESIEEPGGFLDACTAFQNSSVEALELGL 960
            +N+KQDGS+RNY NVPLAKPM EMCEGLD LLESIEEPGGFLDACT FQ SSVEALELGL
Sbjct: 901  SNKKQDGSERNYGNVPLAKPMTEMCEGLDMLLESIEEPGGFLDACTTFQKSSVEALELGL 960

Query: 961  ATLSDQCQIWRRTMNKCAQEVPNLFDKTVQVLQKKTYIEGIVTQASDSNYWEHWDRQKLS 1020
            ATLSDQCQIWRRTM + AQEV NLFD+TV+VL KKTYIEGIVTQASDSNYWEHWDRQKLS
Sbjct: 961  ATLSDQCQIWRRTMTERAQEVQNLFDRTVEVLSKKTYIEGIVTQASDSNYWEHWDRQKLS 1020

Query: 1021 SELELKRQHILKMNQNITNQLIELERHFNGLELNKFGGNDESQVSERALQRKFGSTRHSH 1080
            SELELKRQ IL+MNQN+TNQLIELERHFNGLELNKFGGN+E QV+ER LQRKFGS+R SH
Sbjct: 1021 SELELKRQRILQMNQNMTNQLIELERHFNGLELNKFGGNEEIQVNERTLQRKFGSSRQSH 1080

Query: 1081 SLHSLNNIMGSQLAAAQLLSENLSKQMAALNIESPSLKRQSVTKELFETIGITYDASFSS 1140
            SLHSLNNIMGSQLAAAQLLS+NLSKQ+A LNIESPS KRQS+TKELFETIGITYDASFSS
Sbjct: 1081 SLHSLNNIMGSQLAAAQLLSDNLSKQIATLNIESPSSKRQSITKELFETIGITYDASFSS 1140

Query: 1141 PNVNKIAETSTSKKLLLSADSFSSKDTSRRKQQSGTKNSEAETGRRRRDSLDRRRETTVG 1200
            PNVNKI ETS SKKLLLSADSFSSKDTSRRKQ+SG K SE ETGRRRRDSLDR       
Sbjct: 1141 PNVNKIPETS-SKKLLLSADSFSSKDTSRRKQRSGAKISETETGRRRRDSLDR------- 1200

Query: 1201 GAVRPDLGSGDGESTVRGGGARAGIRAARFVPLQGLDGGEVSRRQGEEGERRAAEEGERR 1260
                                                                        
Sbjct: 1201 ------------------------------------------------------------ 1260

Query: 1261 AANGRRATSCERAASDERTERARRRRNAEGHIRESHVQNALSYLQELLLYYYGFSVLIRH 1320
                                                                        
Sbjct: 1261 ------------------------------------------------------------ 1320

Query: 1321 VYELEIGFMEMAKNLASVEPPKTTVKRMLLQGIPLSGEKQFRSRTPEGVAAVARPASCIT 1380
                         NLASV+PPKTTVKRM+LQG PLS EKQFRS T EG A +ARPAS I 
Sbjct: 1321 -------------NLASVQPPKTTVKRMILQGTPLSNEKQFRSPTLEGPATIARPASRIP 1380

Query: 1381 SSMLSSSSKNAENGSENPATPFTWASPPQPSNSSRQKSQPLQKANATTPSPLPVFQSSHE 1440
            SSMLSSSSKNAE GS+NPATPF+WASPP      RQK QPLQK N T PS LPVFQSSHE
Sbjct: 1381 SSMLSSSSKNAEQGSKNPATPFSWASPP------RQKFQPLQKTNGTAPSSLPVFQSSHE 1440

Query: 1441 MLKKGTNEAYSVTSENKFAEVTFPEKSKSSDFFSLTRNDSVQKPNMNLDQKSSIFTIPAK 1500
            M+KK  +EAYS  SENKFAEVT+PEKSK+SDFFSL R+DSVQK NMN +QKSSIF   +K
Sbjct: 1441 MVKKSNSEAYSAASENKFAEVTYPEKSKASDFFSLARSDSVQKSNMNFEQKSSIFVTSSK 1500

Query: 1501 QTPTPKDSIDTSNSNSQKTANVKERHTTTSPLFGSANKPESAFVGTASSLVSTVDGARKT 1560
               TPKDSI+T N NSQKTANVKER TT SPLFGSANKPE   VGT SSLV TVDG RKT
Sbjct: 1501 PMSTPKDSIETLNPNSQKTANVKERLTTPSPLFGSANKPEPVSVGTTSSLVPTVDGLRKT 1560

Query: 1561 EEKKSTIAFSPSVPAPALFNTPSSASTLFSGFPVSKSLPSSAAVIDLNKPGSTSTQLIFS 1620
            EEKK    FSPSVPAPA  NTP SASTLFSG P+SKS PS AAV+DLNKP STSTQ  F+
Sbjct: 1561 EEKKPPTVFSPSVPAPAPVNTPPSASTLFSGSPLSKSFPSPAAVVDLNKPLSTSTQSSFA 1620

Query: 1621 SPVVSVSDSLFQAPKMVSPSPTLSSLNPTLDSSKKELPVPKSDTDTEKQASASKPESHEL 1680
            SPVVSVSDSLFQAPKMVSP   LSSLNPTL SS KE P+PKSD DTEKQA ASKPES EL
Sbjct: 1621 SPVVSVSDSLFQAPKMVSPPSNLSSLNPTLVSSSKEQPMPKSDADTEKQAPASKPESREL 1679

Query: 1681 KLQPPVTPADKNHVEPTSGTQMVSKDVGGHVPNVIGDAQPQQPSAAFVPLSAPNLTSKIS 1740
            KLQP VT A  NHVEPTS TQ VSKDVGGHVP V+ DAQPQQ  AAFVPL  PN TSK +
Sbjct: 1681 KLQPSVTLAVGNHVEPTSVTQTVSKDVGGHVPFVVADAQPQQSPAAFVPLPTPNSTSKAA 1679

Query: 1741 ANGKNETSDAVVTQDDDMDEEAPETNNNVEFSLSALGGFG-SSSPISSAPKPNPFGGPFG 1800
            ANGK+ETSDA++TQDDDMDEEAPET NNVEFSLS+LGGFG +S+P+S+APKPNPFGG FG
Sbjct: 1741 ANGKSETSDALITQDDDMDEEAPET-NNVEFSLSSLGGFGTTSTPMSNAPKPNPFGGSFG 1679

Query: 1801 NVNATSMNSSFTMAPPQSGELFRPASFSFQSPLASQAASQPTNSVAFSGGFGSGMATQAP 1860
            NVNATSMNSSFT A P SGELFRPASFSFQSPLASQAASQPTN+VAFSG FGSGMATQAP
Sbjct: 1801 NVNATSMNSSFTTASPPSGELFRPASFSFQSPLASQAASQPTNAVAFSGSFGSGMATQAP 1679

Query: 1861 SQGGFGQPAQIGVGQQALGNVLGSFGQSRQLGPSLPGTGSGSPGGFS-GGFTSVKPVGGG 1920
            +QGGFGQPAQIGVGQQALG VLGSFGQSRQLGPSLPGT SGSPGGF+ GGFTSVKPVGGG
Sbjct: 1861 AQGGFGQPAQIGVGQQALGTVLGSFGQSRQLGPSLPGTASGSPGGFNGGGFTSVKPVGGG 1679

Query: 1921 FAGVGTGGGGGFAGVGSGGGGGFGSVGSGGG--------GGFG---SGGFAGAA------ 1980
            FAGVG+GGGGGF G G  GGG  G+  +GGG        GGF     GGFAGAA      
Sbjct: 1921 FAGVGSGGGGGFGGGGFSGGGFAGAASTGGGFAGASPPTGGFAGATGGGFAGAAGGGFAG 1679

Query: 1981 STGGGFAGAASGFGAFGSQQGSSGFSAFAGGGGGAGGTGKPPELFTQIRK 2011
            + GGGFAGAA GFGAFG+QQGS GFSAF    GG+GGTGKPPELFTQIRK
Sbjct: 1981 AAGGGFAGAAGGFGAFGNQQGSGGFSAFGAAPGGSGGTGKPPELFTQIRK 1679

BLAST of Spg009835 vs. ExPASy TrEMBL
Match: A0A6J1HNV2 (nuclear pore complex protein NUP214 isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111466378 PE=4 SV=1)

HSP 1 Score: 2340.8 bits (6065), Expect = 0.0e+00
Identity = 1372/2016 (68.06%), Postives = 1468/2016 (72.82%), Query Frame = 0

Query: 1    MASVDSRHSTSSTQIPLEDGDEGEHVQTTDYYFEKIGEPVPVKLNDSIIDPESPPSQPLA 60
            MASVDSRHSTSST IPLED  EGEHV+T DYYFEKIGEPVPVKLNDSI DP SPPSQPLA
Sbjct: 1    MASVDSRHSTSSTPIPLEDSYEGEHVETNDYYFEKIGEPVPVKLNDSIFDPGSPPSQPLA 60

Query: 61   VSESFGLIFVAHLSGFFVVRTEDVIASAKEMKNGGAGSSVQDLSIVDVSVGKVHILALSS 120
            VSESFGLIFVAHLSGFF VRT+DV+ASAKEMKNGG GSS+QDLSIVDVSVGKVH+LALS+
Sbjct: 61   VSESFGLIFVAHLSGFFAVRTKDVVASAKEMKNGGTGSSIQDLSIVDVSVGKVHVLALSN 120

Query: 121  DNSILAAVVAGDVHLFSVGSLLDKAEKPSSSHSITDTSCIKDFKWTRKLENSYLVLSKHG 180
            DNS LAAVVAGDVHLF V SLLDK E+PS S S TD+SCIKDFKWTRK ENSYLVLSKHG
Sbjct: 121  DNSFLAAVVAGDVHLFLVDSLLDKGEEPSFSCSTTDSSCIKDFKWTRKPENSYLVLSKHG 180

Query: 181  ELYQGSANGPPKHVMHDIDAVECSVKGKFIAVAKKDTLAIFSYKFKERLSMSLLPSLGNG 240
            +LYQGSA+GP KH+MHDIDAVECSVKGKFIAVAKKDTL +FSYKFKERLSMSLLPSLGNG
Sbjct: 181  KLYQGSASGPFKHIMHDIDAVECSVKGKFIAVAKKDTLTVFSYKFKERLSMSLLPSLGNG 240

Query: 241  NTDTDFAVKGSVSVSPSVSLSKNDICKMLKLEVDCIKWVRADCIIIGCFQVTATGDEEDY 300
            +TDTDFAVK                       VD IKWVRADCIIIGCFQVTATGDEEDY
Sbjct: 241  DTDTDFAVK-----------------------VDSIKWVRADCIIIGCFQVTATGDEEDY 300

Query: 301  FVQVVRSKDGKITDVSSNKVLLSFHDIHSGFTHDILPVETGPCLFLSYLDKCKLAIVANR 360
            FVQV+RSKDGKITDVSSNKVLLSFHDI+SGFT DILPVETGPCL LSYLDKCKLAIVANR
Sbjct: 301  FVQVIRSKDGKITDVSSNKVLLSFHDIYSGFTPDILPVETGPCLLLSYLDKCKLAIVANR 360

Query: 361  NNMDQHIVLLGWLLEVENEVAIIDIERDTSLPRINLQVSDRKTMDVLSLLSLIREVVLGP 420
            NN DQHIVLLGWL EVENEVA+IDIERD SLPRI LQ                       
Sbjct: 361  NNTDQHIVLLGWLQEVENEVAVIDIERDKSLPRIELQ----------------------- 420

Query: 421  GRRDVRLWSPNPIEDFSCRLFFRSLLYPSCENGDDNLVMGLCIDRFSLPGKVKVQVGAEE 480
                                          +NGDDNLVMGLCIDR SLPGKV+VQVG EE
Sbjct: 421  ------------------------------DNGDDNLVMGLCIDRVSLPGKVEVQVGNEE 480

Query: 481  TREVSPYCILLCLTLEGKLIMFHFSSVNESEAPHD-VSACDEEEEDGTVEPSDDQSQLSS 540
             REVSPYC LLCLTLEGKLI+FHFSS NESEA  + VSACDEEEED TV P+DDQ QL  
Sbjct: 481  IREVSPYCTLLCLTLEGKLILFHFSSANESEASDETVSACDEEEEDETVVPTDDQPQLF- 540

Query: 541  ESKNEFREAIVSLKMQDTEKIATNSEIPKEKINISSDIKPSNIDQSSVSNIDESVIVSGE 600
                                                    SNIDQ  VS +D S +++ E
Sbjct: 541  ----------------------------------------SNIDQRPVSKVDGSPVITRE 600

Query: 601  SYTKSQKADSFIYSQSLRSSNLERPNNDTGNFDKPVQKFTGLGSVAFSGQSANVPSQSLK 660
            S  KSQ+ DS  +SQ L+ S LERPNN+ GNF KPV+ FTGLGSVAFSGQS +VPSQ LK
Sbjct: 601  SNAKSQQMDSLAFSQPLKPSTLERPNNEIGNFAKPVKNFTGLGSVAFSGQSVDVPSQPLK 660

Query: 661  SSILERPNNEIGNFDKPVQKFTGLGSVAFSGQSANVPSQSLKSSILERPNNEIGNFDKPV 720
            SSILERPNNEIGNF+KP  KFTGLGSVAFSGQS ++P++SLK S LERPNN+IGNF    
Sbjct: 661  SSILERPNNEIGNFNKPFHKFTGLGSVAFSGQSVDMPNESLKPSFLERPNNQIGNF---- 720

Query: 721  QKFTGFGSVTFSGQSAAMPSQSLKSSILERPNNEIGNCDKPVQKFTGLGSVAFSGQSVDV 780
                                                  DKPVQKFTGLGSVAFS QSVDV
Sbjct: 721  --------------------------------------DKPVQKFTGLGSVAFSEQSVDV 780

Query: 781  PSQPFLNVKESTKRLGSTGLQAASELSSDKPMLFKKVDPVSSVLPLNSLQSSKTENYGPS 840
            PS PFLNVKEST +  S                                           
Sbjct: 781  PSHPFLNVKESTIKHSS------------------------------------------- 840

Query: 841  FGAANAFTGFAGKPFQQKDVPSTLTQSERQVTAGSGKIESLPVIRTSQTSLQDNFSTGKT 900
             GAANAFTGFAGKPFQ KDVPSTLTQS RQV+AG+GKIESLPVI++SQ SLQDNFS GK 
Sbjct: 841  -GAANAFTGFAGKPFQPKDVPSTLTQSGRQVSAGAGKIESLPVIQSSQVSLQDNFSLGKI 900

Query: 901  ANEKQDGSDRNYSNVPLAKPMKEMCEGLDKLLESIEEPGGFLDACTAFQNSSVEALELGL 960
            +N+KQDGS+RNY NVPLAKPM EMCEGLD LLESIEEPGGFLDACT FQ SSVEAL LGL
Sbjct: 901  SNKKQDGSERNYGNVPLAKPMNEMCEGLDMLLESIEEPGGFLDACTTFQKSSVEALGLGL 960

Query: 961  ATLSDQCQIWRRTMNKCAQEVPNLFDKTVQVLQKKTYIEGIVTQASDSNYWEHWDRQKLS 1020
            ATLSDQCQIWRRTM + AQEV NLFD+TV+VL KKTYIEGIVTQASDSNYW+HWDRQKLS
Sbjct: 961  ATLSDQCQIWRRTMTERAQEVQNLFDRTVEVLSKKTYIEGIVTQASDSNYWDHWDRQKLS 1020

Query: 1021 SELELKRQHILKMNQNITNQLIELERHFNGLELNKFGGNDESQVSERALQRKFGSTRHSH 1080
            SELELKRQ IL+MNQN+TNQLIELERHFNGLELN FGGN+E QV+ER LQRKFGS+R SH
Sbjct: 1021 SELELKRQRILQMNQNMTNQLIELERHFNGLELNTFGGNEEIQVNERTLQRKFGSSRQSH 1080

Query: 1081 SLHSLNNIMGSQLAAAQLLSENLSKQMAALNIESPSLKRQSVTKELFETIGITYDASFSS 1140
            SLHSLNNIMGSQLAAAQLLS+NLSKQ+A LNI+SPS KRQS+TKELFETIGITYDASFSS
Sbjct: 1081 SLHSLNNIMGSQLAAAQLLSDNLSKQIATLNIKSPSSKRQSITKELFETIGITYDASFSS 1140

Query: 1141 PNVNKIAETSTSKKLLLSADSFSSKDTSRRKQQSGTKNSEAETGRRRRDSLDRRRETTVG 1200
            PNVNKI ETS SKKLLLSADSFSSKDTSRRKQ+SG K SE ETGRRRRDSLDR       
Sbjct: 1141 PNVNKIPETS-SKKLLLSADSFSSKDTSRRKQRSGAKISETETGRRRRDSLDR------- 1200

Query: 1201 GAVRPDLGSGDGESTVRGGGARAGIRAARFVPLQGLDGGEVSRRQGEEGERRAAEEGERR 1260
                                                                        
Sbjct: 1201 ------------------------------------------------------------ 1260

Query: 1261 AANGRRATSCERAASDERTERARRRRNAEGHIRESHVQNALSYLQELLLYYYGFSVLIRH 1320
                                                                        
Sbjct: 1261 ------------------------------------------------------------ 1320

Query: 1321 VYELEIGFMEMAKNLASVEPPKTTVKRMLLQGIPLSGEKQFRSRTPEGVAAVARPASCIT 1380
                         NLAS++PPKTTVKRM+LQG PLS EKQFRS T EG A VARPA  I 
Sbjct: 1321 -------------NLASIQPPKTTVKRMILQGTPLSNEKQFRSPTLEGPATVARPAGRIP 1380

Query: 1381 SSMLSSSSKNAENGSENPATPFTWASPPQPSNSSRQKSQPLQKANATTPSPLPVFQSSHE 1440
            SSMLSSSSKNAE GSENPATPF+WASPP      RQK QPLQK N T PSPLPV+QSSHE
Sbjct: 1381 SSMLSSSSKNAEQGSENPATPFSWASPP------RQKFQPLQKTNGTAPSPLPVYQSSHE 1440

Query: 1441 MLKKGTNEAYSVTSENKFAEVTFPEKSKSSDFFSLTRNDSVQKPNMNLDQKSSIFTIPAK 1500
            M+KK  +EAYS  SENKFAEVT+PEKSK+SDFFSL R+DSVQK NMN +QKSS F   +K
Sbjct: 1441 MVKKSNSEAYSAASENKFAEVTYPEKSKASDFFSLARSDSVQKSNMNFEQKSSFFVTSSK 1500

Query: 1501 QTPTPKDSIDTSNSNSQKTANVKERHTTTSPLFGSANKPESAFVGTASSLVSTVDGARKT 1560
               TPKDSI+T N NSQKTANVKER TT SPLFG+ANKPE A VGT SSLV TVD  RKT
Sbjct: 1501 PMSTPKDSIETLNPNSQKTANVKERLTTPSPLFGAANKPEPASVGTTSSLVPTVDELRKT 1560

Query: 1561 EEKKSTIAFSPSVPAPALFNTPSSASTLFSGFPVSKSLPSSAAVIDLNKPGSTSTQLIFS 1620
            EEKK    FSPSVPA    NTPSSASTLFSG P+SKS PS AAV+DLNKP STSTQ  F+
Sbjct: 1561 EEKKPPTVFSPSVPASVPVNTPSSASTLFSGSPLSKSFPSPAAVVDLNKPLSTSTQSSFA 1620

Query: 1621 SPVVSVSDSLFQAPKMVSPSPTLSSLNPTLDSSKKELPVPKSDTDTEKQASASKPESHEL 1680
            SPVVSVSDSLFQAPKMVSP  TLSSLNP+L SS KE P+PKSD DTEKQA ASKPE  EL
Sbjct: 1621 SPVVSVSDSLFQAPKMVSPPSTLSSLNPSLVSSSKEQPIPKSDADTEKQAQASKPEFREL 1663

Query: 1681 KLQPPVTPADKNHVEPTSGTQMVSKDVGGHVPNVIGDAQPQQPSAAFVPLSAPNLTSKIS 1740
            KLQP VT A  NHVEPTS TQ VSKDVGGHVP+VI DAQPQQ SAAFVPL +PN T K+S
Sbjct: 1681 KLQPSVTLAVGNHVEPTSVTQTVSKDVGGHVPSVIADAQPQQSSAAFVPLPSPNSTPKVS 1663

Query: 1741 ANGKNETSDAVVTQDDDMDEEAPETNNNVEFSLSALGGFG-SSSPISSAPKPNPFGGPFG 1800
            ANGK+ETSDA++TQDDDMDEEAPET NNVEFSLS+LGGFG +S+P+S+APKPNPFGG FG
Sbjct: 1741 ANGKSETSDALITQDDDMDEEAPET-NNVEFSLSSLGGFGTTSTPMSNAPKPNPFGGSFG 1663

Query: 1801 NVNATSMNSSFTMAPPQSGELFRPASFSFQSPLASQAASQPTNSVAFSGGFGSGMATQAP 1860
            N NATSMNSSFT A P SGELFRPASFSFQSPLASQAASQPTNSVAFS  FGSGMATQAP
Sbjct: 1801 NANATSMNSSFTTASPPSGELFRPASFSFQSPLASQAASQPTNSVAFSSSFGSGMATQAP 1663

Query: 1861 SQGGFGQPAQIGVGQQALGNVLGSFGQSRQLGPSLPGTGSGSPGGFS-GGFTSVKPVGGG 1920
            +QGGFGQPAQIGVGQQALG VLGSFGQSRQLGPSLPGT SGSPGGF+ GGFTSVKPVGGG
Sbjct: 1861 TQGGFGQPAQIGVGQQALGTVLGSFGQSRQLGPSLPGTASGSPGGFNGGGFTSVKPVGGG 1663

Query: 1921 FAGVGTGGGGGFAGVGSGGGGGFGSVGSGGGGGFGS---GGFAGAASTGGGFAGAASGFG 1980
            FAGVG+GGGGGF G G GGGG  G+  +GGG    S   GGFAGAA  GGGFAGAA GFG
Sbjct: 1921 FAGVGSGGGGGFGGGGFGGGGFAGAASTGGGFAGASPPTGGFAGAA--GGGFAGAAGGFG 1663

Query: 1981 AFGSQQGSSGFSAFAGGGGGAGGTGKPPELFTQIRK 2011
            AFG+QQGS GFSAF    GG+GGTGKPPELFTQIRK
Sbjct: 1981 AFGNQQGSGGFSAFGAAPGGSGGTGKPPELFTQIRK 1663

BLAST of Spg009835 vs. ExPASy TrEMBL
Match: A0A6J1HQ79 (nuclear pore complex protein NUP214 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111466378 PE=4 SV=1)

HSP 1 Score: 2340.5 bits (6064), Expect = 0.0e+00
Identity = 1374/2021 (67.99%), Postives = 1469/2021 (72.69%), Query Frame = 0

Query: 1    MASVDSRHSTSSTQIPLEDGDEGEHVQTTDYYFEKIGEPVPVKLNDSIIDPESPPSQPLA 60
            MASVDSRHSTSST IPLED  EGEHV+T DYYFEKIGEPVPVKLNDSI DP SPPSQPLA
Sbjct: 1    MASVDSRHSTSSTPIPLEDSYEGEHVETNDYYFEKIGEPVPVKLNDSIFDPGSPPSQPLA 60

Query: 61   VSESFGLIFVAHLSGFFVVRTEDVIASAKEMKNGGAGSSVQDLSIVDVSVGKVHILALSS 120
            VSESFGLIFVAHLSGFF VRT+DV+ASAKEMKNGG GSS+QDLSIVDVSVGKVH+LALS+
Sbjct: 61   VSESFGLIFVAHLSGFFAVRTKDVVASAKEMKNGGTGSSIQDLSIVDVSVGKVHVLALSN 120

Query: 121  DNSILAAVVAGDVHLFSVGSLLDKAEKPSSSHSITDTSCIKDFKWTRKLENSYLVLSKHG 180
            DNS LAAVVAGDVHLF V SLLDK E+PS S S TD+SCIKDFKWTRK ENSYLVLSKHG
Sbjct: 121  DNSFLAAVVAGDVHLFLVDSLLDKGEEPSFSCSTTDSSCIKDFKWTRKPENSYLVLSKHG 180

Query: 181  ELYQGSANGPPKHVMHDIDAVECSVKGKFIAVAKKDTLAIFSYKFKERLSMSLLPSLGNG 240
            +LYQGSA+GP KH+MHDIDAVECSVKGKFIAVAKKDTL +FSYKFKERLSMSLLPSLGNG
Sbjct: 181  KLYQGSASGPFKHIMHDIDAVECSVKGKFIAVAKKDTLTVFSYKFKERLSMSLLPSLGNG 240

Query: 241  NTDTDFAVKGSVSVSPSVSLSKNDICKMLKLEVDCIKWVRADCIIIGCFQVTATGDEEDY 300
            +TDTDFAVK                       VD IKWVRADCIIIGCFQVTATGDEEDY
Sbjct: 241  DTDTDFAVK-----------------------VDSIKWVRADCIIIGCFQVTATGDEEDY 300

Query: 301  FVQVVRSKDGKITDVSSNKVLLSFHDIHSGFTHDILPVETGPCLFLSYLDKCKLAIVANR 360
            FVQV+RSKDGKITDVSSNKVLLSFHDI+SGFT DILPVETGPCL LSYLDKCKLAIVANR
Sbjct: 301  FVQVIRSKDGKITDVSSNKVLLSFHDIYSGFTPDILPVETGPCLLLSYLDKCKLAIVANR 360

Query: 361  NNMDQHIVLLGWLLEVENEVAIIDIERDTSLPRINLQVSDRKTMDVLSLLSLIREVVLGP 420
            NN DQHIVLLGWL EVENEVA+IDIERD SLPRI LQ                       
Sbjct: 361  NNTDQHIVLLGWLQEVENEVAVIDIERDKSLPRIELQ----------------------- 420

Query: 421  GRRDVRLWSPNPIEDFSCRLFFRSLLYPSCENGDDNLVMGLCIDRFSLPGKVKVQVGAEE 480
                                          +NGDDNLVMGLCIDR SLPGKV+VQVG EE
Sbjct: 421  ------------------------------DNGDDNLVMGLCIDRVSLPGKVEVQVGNEE 480

Query: 481  TREVSPYCILLCLTLEGKLIMFHFSSVNESEAPHD-VSACDEEEEDGTVEPSDDQSQLSS 540
             REVSPYC LLCLTLEGKLI+FHFSS NESEA  + VSACDEEEED TV P+DDQ QL  
Sbjct: 481  IREVSPYCTLLCLTLEGKLILFHFSSANESEASDETVSACDEEEEDETVVPTDDQPQLF- 540

Query: 541  ESKNEFREAIVSLKMQDTEKIATNSEIPKEKINISSDIKPSNIDQSSVSNIDESVIVSGE 600
                                                    SNIDQ  VS +D S +++ E
Sbjct: 541  ----------------------------------------SNIDQRPVSKVDGSPVITRE 600

Query: 601  SYTKSQKADSFIYSQSLRSSNLERPNNDTGNFDKPVQKFTGLGSVAFSGQSANVPSQSLK 660
            S  KSQ+ DS  +SQ L+ S LERPNN+ GNF KPV+ FTGLGSVAFSGQS +VPSQ LK
Sbjct: 601  SNAKSQQMDSLAFSQPLKPSTLERPNNEIGNFAKPVKNFTGLGSVAFSGQSVDVPSQPLK 660

Query: 661  SSILERPNNEIGNFDKPVQKFTGLGSVAFSGQSANVPSQSLKSSILERPNNEIGNFDKPV 720
            SSILERPNNEIGNF+KP  KFTGLGSVAFSGQS ++P++SLK S LERPNN+IGNF    
Sbjct: 661  SSILERPNNEIGNFNKPFHKFTGLGSVAFSGQSVDMPNESLKPSFLERPNNQIGNF---- 720

Query: 721  QKFTGFGSVTFSGQSAAMPSQSLKSSILERPNNEIGNCDKPVQKFTGLGSVAFSGQSVDV 780
                                                  DKPVQKFTGLGSVAFS QSVDV
Sbjct: 721  --------------------------------------DKPVQKFTGLGSVAFSEQSVDV 780

Query: 781  PSQPFLNVKESTKRLGSTGLQAASELSSDKPMLFKKVDPVSSVLPLNSLQSSKTENYGPS 840
            PS PFLNVKEST +  S                                           
Sbjct: 781  PSHPFLNVKESTIKHSS------------------------------------------- 840

Query: 841  FGAANAFTGFAGKPFQQKDVPSTLTQSERQVTAGSGKIESLPVIRTSQTSLQDNFSTGKT 900
             GAANAFTGFAGKPFQ KDVPSTLTQS RQV+AG+GKIESLPVI++SQ SLQDNFS GK 
Sbjct: 841  -GAANAFTGFAGKPFQPKDVPSTLTQSGRQVSAGAGKIESLPVIQSSQVSLQDNFSLGKI 900

Query: 901  ANEKQDGSDRNYSNVPLAKPMKEMCEGLDKLLESIEEPGGFLDACTAFQNSSVEALELGL 960
            +N+KQDGS+RNY NVPLAKPM EMCEGLD LLESIEEPGGFLDACT FQ SSVEAL LGL
Sbjct: 901  SNKKQDGSERNYGNVPLAKPMNEMCEGLDMLLESIEEPGGFLDACTTFQKSSVEALGLGL 960

Query: 961  ATLSDQCQIWRRTMNKCAQEVPNLFDKTVQVLQKKTYIEGIVTQASDSNYWEHWDRQKLS 1020
            ATLSDQCQIWRRTM + AQEV NLFD+TV+VL KKTYIEGIVTQASDSNYW+HWDRQKLS
Sbjct: 961  ATLSDQCQIWRRTMTERAQEVQNLFDRTVEVLSKKTYIEGIVTQASDSNYWDHWDRQKLS 1020

Query: 1021 SELELKRQHILKMNQNITNQLIELERHFNGLELNKFGGNDESQVSERALQRKFGSTRHSH 1080
            SELELKRQ IL+MNQN+TNQLIELERHFNGLELN FGGN+E QV+ER LQRKFGS+R SH
Sbjct: 1021 SELELKRQRILQMNQNMTNQLIELERHFNGLELNTFGGNEEIQVNERTLQRKFGSSRQSH 1080

Query: 1081 SLHSLNNIMGSQLAAAQLLSENLSKQMAALNIESPSLKRQSVTKELFETIGITYDASFSS 1140
            SLHSLNNIMGSQLAAAQLLS+NLSKQ+A LNI+SPS KRQS+TKELFETIGITYDASFSS
Sbjct: 1081 SLHSLNNIMGSQLAAAQLLSDNLSKQIATLNIKSPSSKRQSITKELFETIGITYDASFSS 1140

Query: 1141 PNVNKIAETSTSKKLLLSADSFSSKDTSRRKQQSGTKNSEAETGRRRRDSLDRRRETTVG 1200
            PNVNKI ETS SKKLLLSADSFSSKDTSRRKQ+SG K SE ETGRRRRDSLDR       
Sbjct: 1141 PNVNKIPETS-SKKLLLSADSFSSKDTSRRKQRSGAKISETETGRRRRDSLDR------- 1200

Query: 1201 GAVRPDLGSGDGESTVRGGGARAGIRAARFVPLQGLDGGEVSRRQGEEGERRAAEEGERR 1260
                                                                        
Sbjct: 1201 ------------------------------------------------------------ 1260

Query: 1261 AANGRRATSCERAASDERTERARRRRNAEGHIRESHVQNALSYLQELLLYYYGFSVLIRH 1320
                                                                        
Sbjct: 1261 ------------------------------------------------------------ 1320

Query: 1321 VYELEIGFMEMAKNLASVEPPKTTVKRMLLQGIPLSGEKQFRSRTPEGVAAVARPASCIT 1380
                         NLAS++PPKTTVKRM+LQG PLS EKQFRS T EG A VARPA  I 
Sbjct: 1321 -------------NLASIQPPKTTVKRMILQGTPLSNEKQFRSPTLEGPATVARPAGRIP 1380

Query: 1381 SSMLSSSSKNAENGSENPATPFTWASPPQPSNSSRQKSQPLQKANATTPSPLPVFQSSHE 1440
            SSMLSSSSKNAE GSENPATPF+WASPP      RQK QPLQK N T PSPLPV+QSSHE
Sbjct: 1381 SSMLSSSSKNAEQGSENPATPFSWASPP------RQKFQPLQKTNGTAPSPLPVYQSSHE 1440

Query: 1441 MLKKGTNEAYSVTSENKFAEVTFPEKSKSSDFFSLTRNDSVQKPNMNLDQKSSIFTIPAK 1500
            M+KK  +EAYS  SENKFAEVT+PEKSK+SDFFSL R+DSVQK NMN +QKSS F   +K
Sbjct: 1441 MVKKSNSEAYSAASENKFAEVTYPEKSKASDFFSLARSDSVQKSNMNFEQKSSFFVTSSK 1500

Query: 1501 QTPTPKDSIDTSNSNSQKTANVKERHTTTSPLFGSANKPESAFVGTASSLVSTVDGARKT 1560
               TPKDSI+T N NSQKTANVKER TT SPLFG+ANKPE A VGT SSLV TVD  RKT
Sbjct: 1501 PMSTPKDSIETLNPNSQKTANVKERLTTPSPLFGAANKPEPASVGTTSSLVPTVDELRKT 1560

Query: 1561 EEKKSTIAFSPSVPAPALFNTPSSASTLFSGFPVSKSLPSSAAVIDLNKPGSTSTQLIFS 1620
            EEKK    FSPSVPA    NTPSSASTLFSG P+SKS PS AAV+DLNKP STSTQ  F+
Sbjct: 1561 EEKKPPTVFSPSVPASVPVNTPSSASTLFSGSPLSKSFPSPAAVVDLNKPLSTSTQSSFA 1620

Query: 1621 SPVVSVSDSLFQAPKMVSPSPTLSSLNPTLDSSKKELPVPKSDTDTEKQASASKPESHEL 1680
            SPVVSVSDSLFQAPKMVSP  TLSSLNP+L SS KE P+PKSD DTEKQA ASKPE  EL
Sbjct: 1621 SPVVSVSDSLFQAPKMVSPPSTLSSLNPSLVSSSKEQPIPKSDADTEKQAQASKPEFREL 1668

Query: 1681 KLQPPVTPADKNHVEPTSGTQMVSKDVGGHVPNVIGDAQPQQPSAAFVPLSAPNLTSKIS 1740
            KLQP VT A  NHVEPTS TQ VSKDVGGHVP+VI DAQPQQ SAAFVPL +PN T K+S
Sbjct: 1681 KLQPSVTLAVGNHVEPTSVTQTVSKDVGGHVPSVIADAQPQQSSAAFVPLPSPNSTPKVS 1668

Query: 1741 ANGKNETSDAVVTQDDDMDEEAPETNNNVEFSLSALGGFG-SSSPISSAPKPNPFGGPFG 1800
            ANGK+ETSDA++TQDDDMDEEAPET NNVEFSLS+LGGFG +S+P+S+APKPNPFGG FG
Sbjct: 1741 ANGKSETSDALITQDDDMDEEAPET-NNVEFSLSSLGGFGTTSTPMSNAPKPNPFGGSFG 1668

Query: 1801 NVNATSMNSSFTMAPPQSGELFRPASFSFQSPLASQAASQPTNSVAFSGGFGSGMATQAP 1860
            N NATSMNSSFT A P SGELFRPASFSFQSPLASQAASQPTNSVAFS  FGSGMATQAP
Sbjct: 1801 NANATSMNSSFTTASPPSGELFRPASFSFQSPLASQAASQPTNSVAFSSSFGSGMATQAP 1668

Query: 1861 SQGGFGQPAQIGVGQQALGNVLGSFGQSRQLGPSLPGTGSGSPGGFS-GGFTSVKPVGGG 1920
            +QGGFGQPAQIGVGQQALG VLGSFGQSRQLGPSLPGT SGSPGGF+ GGFTSVKPVGGG
Sbjct: 1861 TQGGFGQPAQIGVGQQALGTVLGSFGQSRQLGPSLPGTASGSPGGFNGGGFTSVKPVGGG 1668

Query: 1921 FAGVGTGGGGGFAGVGSG----GGGGFGSVGSGGGGGFG----SGGFAGAASTGGGFAGA 1980
            FAGVG+GGGGGF G G G    GGGGF    S GGG  G    +GGFAGAA  GGGFAGA
Sbjct: 1921 FAGVGSGGGGGFGGGGFGGGGFGGGGFAGAASTGGGFAGASPPTGGFAGAA--GGGFAGA 1668

Query: 1981 ASGFGAFGSQQGSSGFSAFAGGGGGAGGTGKPPELFTQIRK 2011
            A GFGAFG+QQGS GFSAF    GG+GGTGKPPELFTQIRK
Sbjct: 1981 AGGFGAFGNQQGSGGFSAFGAAPGGSGGTGKPPELFTQIRK 1668

BLAST of Spg009835 vs. ExPASy TrEMBL
Match: A0A6J1HUR6 (nuclear pore complex protein NUP214 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111466378 PE=4 SV=1)

HSP 1 Score: 2337.8 bits (6057), Expect = 0.0e+00
Identity = 1373/2029 (67.67%), Postives = 1471/2029 (72.50%), Query Frame = 0

Query: 1    MASVDSRHSTSSTQIPLEDGDEGEHVQTTDYYFEKIGEPVPVKLNDSIIDPESPPSQPLA 60
            MASVDSRHSTSST IPLED  EGEHV+T DYYFEKIGEPVPVKLNDSI DP SPPSQPLA
Sbjct: 1    MASVDSRHSTSSTPIPLEDSYEGEHVETNDYYFEKIGEPVPVKLNDSIFDPGSPPSQPLA 60

Query: 61   VSESFGLIFVAHLSGFFVVRTEDVIASAKEMKNGGAGSSVQDLSIVDVSVGKVHILALSS 120
            VSESFGLIFVAHLSGFF VRT+DV+ASAKEMKNGG GSS+QDLSIVDVSVGKVH+LALS+
Sbjct: 61   VSESFGLIFVAHLSGFFAVRTKDVVASAKEMKNGGTGSSIQDLSIVDVSVGKVHVLALSN 120

Query: 121  DNSILAAVVAGDVHLFSVGSLLDKAEKPSSSHSITDTSCIKDFKWTRKLENSYLVLSKHG 180
            DNS LAAVVAGDVHLF V SLLDK E+PS S S TD+SCIKDFKWTRK ENSYLVLSKHG
Sbjct: 121  DNSFLAAVVAGDVHLFLVDSLLDKGEEPSFSCSTTDSSCIKDFKWTRKPENSYLVLSKHG 180

Query: 181  ELYQGSANGPPKHVMHDIDAVECSVKGKFIAVAKKDTLAIFSYKFKERLSMSLLPSLGNG 240
            +LYQGSA+GP KH+MHDIDAVECSVKGKFIAVAKKDTL +FSYKFKERLSMSLLPSLGNG
Sbjct: 181  KLYQGSASGPFKHIMHDIDAVECSVKGKFIAVAKKDTLTVFSYKFKERLSMSLLPSLGNG 240

Query: 241  NTDTDFAVKGSVSVSPSVSLSKNDICKMLKLEVDCIKWVRADCIIIGCFQVTATGDEEDY 300
            +TDTDFAVK                       VD IKWVRADCIIIGCFQVTATGDEEDY
Sbjct: 241  DTDTDFAVK-----------------------VDSIKWVRADCIIIGCFQVTATGDEEDY 300

Query: 301  FVQVVRSKDGKITDVSSNKVLLSFHDIHSGFTHDILPVETGPCLFLSYLDKCKLAIVANR 360
            FVQV+RSKDGKITDVSSNKVLLSFHDI+SGFT DILPVETGPCL LSYLDKCKLAIVANR
Sbjct: 301  FVQVIRSKDGKITDVSSNKVLLSFHDIYSGFTPDILPVETGPCLLLSYLDKCKLAIVANR 360

Query: 361  NNMDQHIVLLGWLLEVENEVAIIDIERDTSLPRINLQVSDRKTMDVLSLLSLIREVVLGP 420
            NN DQHIVLLGWL EVENEVA+IDIERD SLPRI LQ                       
Sbjct: 361  NNTDQHIVLLGWLQEVENEVAVIDIERDKSLPRIELQ----------------------- 420

Query: 421  GRRDVRLWSPNPIEDFSCRLFFRSLLYPSCENGDDNLVMGLCIDRFSLPGKVKVQVGAEE 480
                                          +NGDDNLVMGLCIDR SLPGKV+VQVG EE
Sbjct: 421  ------------------------------DNGDDNLVMGLCIDRVSLPGKVEVQVGNEE 480

Query: 481  TREVSPYCILLCLTLEGKLIMFHFSSVNESEAPHD-VSACDEEEEDGTVEPSDDQSQLSS 540
             REVSPYC LLCLTLEGKLI+FHFSS NESEA  + VSACDEEEED TV P+DDQ QL  
Sbjct: 481  IREVSPYCTLLCLTLEGKLILFHFSSANESEASDETVSACDEEEEDETVVPTDDQPQLF- 540

Query: 541  ESKNEFREAIVSLKMQDTEKIATNSEIPKEKINISSDIKPSNIDQSSVSNIDESVIVSGE 600
                                                    SNIDQ  VS +D S +++ E
Sbjct: 541  ----------------------------------------SNIDQRPVSKVDGSPVITRE 600

Query: 601  SYTKSQKADSFIYSQSLRSSNLERPNNDTGNFDKPVQKFTGLGSVAFSGQSANVPSQSLK 660
            S  KSQ+ DS  +SQ L+ S LERPNN+ GNF KPV+ FTGLGSVAFSGQS +VPSQ LK
Sbjct: 601  SNAKSQQMDSLAFSQPLKPSTLERPNNEIGNFAKPVKNFTGLGSVAFSGQSVDVPSQPLK 660

Query: 661  SSILERPNNEIGNFDKPVQKFTGLGSVAFSGQSANVPSQSLKSSILERPNNEIGNFDKPV 720
            SSILERPNNEIGNF+KP  KFTGLGSVAFSGQS ++P++SLK S LERPNN+IGNF    
Sbjct: 661  SSILERPNNEIGNFNKPFHKFTGLGSVAFSGQSVDMPNESLKPSFLERPNNQIGNF---- 720

Query: 721  QKFTGFGSVTFSGQSAAMPSQSLKSSILERPNNEIGNCDKPVQKFTGLGSVAFSGQSVDV 780
                                                  DKPVQKFTGLGSVAFS QSVDV
Sbjct: 721  --------------------------------------DKPVQKFTGLGSVAFSEQSVDV 780

Query: 781  PSQPFLNVKESTKRLGSTGLQAASELSSDKPMLFKKVDPVSSVLPLNSLQSSKTENYGPS 840
            PS PFLNVKEST +  S                                           
Sbjct: 781  PSHPFLNVKESTIKHSS------------------------------------------- 840

Query: 841  FGAANAFTGFAGKPFQQKDVPSTLTQSERQVTAGSGKIESLPVIRTSQTSLQDNFSTGKT 900
             GAANAFTGFAGKPFQ KDVPSTLTQS RQV+AG+GKIESLPVI++SQ SLQDNFS GK 
Sbjct: 841  -GAANAFTGFAGKPFQPKDVPSTLTQSGRQVSAGAGKIESLPVIQSSQVSLQDNFSLGKI 900

Query: 901  ANEKQDGSDRNYSNVPLAKPMKEMCEGLDKLLESIEEPGGFLDACTAFQNSSVEALELGL 960
            +N+KQDGS+RNY NVPLAKPM EMCEGLD LLESIEEPGGFLDACT FQ SSVEAL LGL
Sbjct: 901  SNKKQDGSERNYGNVPLAKPMNEMCEGLDMLLESIEEPGGFLDACTTFQKSSVEALGLGL 960

Query: 961  ATLSDQCQIWRRTMNKCAQEVPNLFDKTVQVLQKKTYIEGIVTQASDSNYWEHWDRQKLS 1020
            ATLSDQCQIWRRTM + AQEV NLFD+TV+VL KKTYIEGIVTQASDSNYW+HWDRQKLS
Sbjct: 961  ATLSDQCQIWRRTMTERAQEVQNLFDRTVEVLSKKTYIEGIVTQASDSNYWDHWDRQKLS 1020

Query: 1021 SELELKRQHILKMNQNITNQLIELERHFNGLELNKFGGNDESQVSERALQRKFGSTRHSH 1080
            SELELKRQ IL+MNQN+TNQLIELERHFNGLELN FGGN+E QV+ER LQRKFGS+R SH
Sbjct: 1021 SELELKRQRILQMNQNMTNQLIELERHFNGLELNTFGGNEEIQVNERTLQRKFGSSRQSH 1080

Query: 1081 SLHSLNNIMGSQLAAAQLLSENLSKQMAALNIESPSLKRQSVTKELFETIGITYDASFSS 1140
            SLHSLNNIMGSQLAAAQLLS+NLSKQ+A LNI+SPS KRQS+TKELFETIGITYDASFSS
Sbjct: 1081 SLHSLNNIMGSQLAAAQLLSDNLSKQIATLNIKSPSSKRQSITKELFETIGITYDASFSS 1140

Query: 1141 PNVNKIAETSTSKKLLLSADSFSSKDTSRRKQQSGTKNSEAETGRRRRDSLDRRRETTVG 1200
            PNVNKI ETS SKKLLLSADSFSSKDTSRRKQ+SG K SE ETGRRRRDSLDR       
Sbjct: 1141 PNVNKIPETS-SKKLLLSADSFSSKDTSRRKQRSGAKISETETGRRRRDSLDR------- 1200

Query: 1201 GAVRPDLGSGDGESTVRGGGARAGIRAARFVPLQGLDGGEVSRRQGEEGERRAAEEGERR 1260
                                                                        
Sbjct: 1201 ------------------------------------------------------------ 1260

Query: 1261 AANGRRATSCERAASDERTERARRRRNAEGHIRESHVQNALSYLQELLLYYYGFSVLIRH 1320
                                                                        
Sbjct: 1261 ------------------------------------------------------------ 1320

Query: 1321 VYELEIGFMEMAKNLASVEPPKTTVKRMLLQGIPLSGEKQFRSRTPEGVAAVARPASCIT 1380
                         NLAS++PPKTTVKRM+LQG PLS EKQFRS T EG A VARPA  I 
Sbjct: 1321 -------------NLASIQPPKTTVKRMILQGTPLSNEKQFRSPTLEGPATVARPAGRIP 1380

Query: 1381 SSMLSSSSKNAENGSENPATPFTWASPPQPSNSSRQKSQPLQKANATTPSPLPVFQSSHE 1440
            SSMLSSSSKNAE GSENPATPF+WASPP      RQK QPLQK N T PSPLPV+QSSHE
Sbjct: 1381 SSMLSSSSKNAEQGSENPATPFSWASPP------RQKFQPLQKTNGTAPSPLPVYQSSHE 1440

Query: 1441 MLKKGTNEAYSVTSENKFAEVTFPEKSKSSDFFSLTRNDSVQKPNMNLDQKSSIFTIPAK 1500
            M+KK  +EAYS  SENKFAEVT+PEKSK+SDFFSL R+DSVQK NMN +QKSS F   +K
Sbjct: 1441 MVKKSNSEAYSAASENKFAEVTYPEKSKASDFFSLARSDSVQKSNMNFEQKSSFFVTSSK 1500

Query: 1501 QTPTPKDSIDTSNSNSQKTANVKERHTTTSPLFGSANKPESAFVGTASSLVSTVDGARKT 1560
               TPKDSI+T N NSQKTANVKER TT SPLFG+ANKPE A VGT SSLV TVD  RKT
Sbjct: 1501 PMSTPKDSIETLNPNSQKTANVKERLTTPSPLFGAANKPEPASVGTTSSLVPTVDELRKT 1560

Query: 1561 EEKKSTIAFSPSVPAPALFNTPSSASTLFSGFPVSKSLPSSAAVIDLNKPGSTSTQLIFS 1620
            EEKK    FSPSVPA    NTPSSASTLFSG P+SKS PS AAV+DLNKP STSTQ  F+
Sbjct: 1561 EEKKPPTVFSPSVPASVPVNTPSSASTLFSGSPLSKSFPSPAAVVDLNKPLSTSTQSSFA 1620

Query: 1621 SPVVSVSDSLFQAPKMVSPSPTLSSLNPTLDSSKKELPVPKSDTDTEKQASASKPESHEL 1680
            SPVVSVSDSLFQAPKMVSP  TLSSLNP+L SS KE P+PKSD DTEKQA ASKPE  EL
Sbjct: 1621 SPVVSVSDSLFQAPKMVSPPSTLSSLNPSLVSSSKEQPIPKSDADTEKQAQASKPEFREL 1678

Query: 1681 KLQPPVTPADKNHVEPTSGTQMVSKDVGGHVPNVIGDAQPQQPSAAFVPLSAPNLTSKIS 1740
            KLQP VT A  NHVEPTS TQ VSKDVGGHVP+VI DAQPQQ SAAFVPL +PN T K+S
Sbjct: 1681 KLQPSVTLAVGNHVEPTSVTQTVSKDVGGHVPSVIADAQPQQSSAAFVPLPSPNSTPKVS 1678

Query: 1741 ANGKNETSDAVVTQDDDMDEEAPETNNNVEFSLSALGGFG-SSSPISSAPKPNPFGGPFG 1800
            ANGK+ETSDA++TQDDDMDEEAPET NNVEFSLS+LGGFG +S+P+S+APKPNPFGG FG
Sbjct: 1741 ANGKSETSDALITQDDDMDEEAPET-NNVEFSLSSLGGFGTTSTPMSNAPKPNPFGGSFG 1678

Query: 1801 NVNATSMNSSFTMAPPQSGELFRPASFSFQSPLASQAASQPTNSVAFSGGFGSGMATQAP 1860
            N NATSMNSSFT A P SGELFRPASFSFQSPLASQAASQPTNSVAFS  FGSGMATQAP
Sbjct: 1801 NANATSMNSSFTTASPPSGELFRPASFSFQSPLASQAASQPTNSVAFSSSFGSGMATQAP 1678

Query: 1861 SQGGFGQPAQIGVGQQALGNVLGSFGQSRQLGPSLPGTGSGSPGGFS-GGFTSVKPVGGG 1920
            +QGGFGQPAQIGVGQQALG VLGSFGQSRQLGPSLPGT SGSPGGF+ GGFTSVKPVGGG
Sbjct: 1861 TQGGFGQPAQIGVGQQALGTVLGSFGQSRQLGPSLPGTASGSPGGFNGGGFTSVKPVGGG 1678

Query: 1921 FAGVGTGGGGGFAGVGSG----GGGGFGSVGSGGGGGFGS----GGFAGAA--------S 1980
            FAGVG+GGGGGF G G G    GGGGF +  S GGG  G+    GGFAGA+        +
Sbjct: 1921 FAGVGSGGGGGFGGGGFGGGGFGGGGFAAAASTGGGFAGAASTGGGFAGASPPTGGFAGA 1678

Query: 1981 TGGGFAGAASGFGAFGSQQGSSGFSAFAGGGGGAGGTGKPPELFTQIRK 2011
             GGGFAGAA GFGAFG+QQGS GFSAF    GG+GGTGKPPELFTQIRK
Sbjct: 1981 AGGGFAGAAGGFGAFGNQQGSGGFSAFGAAPGGSGGTGKPPELFTQIRK 1678

BLAST of Spg009835 vs. TAIR 10
Match: AT1G55540.1 (Nuclear pore complex protein )

HSP 1 Score: 775.0 bits (2000), Expect = 1.5e-223
Identity = 738/2123 (34.76%), Postives = 1021/2123 (48.09%), Query Frame = 0

Query: 13   TQIPLEDGDEGEHVQTTDYYFEKIGEPVPVKLNDSIIDPESPPSQPLAVSESFGLIFVAH 72
            +++ +E+  EG+ + T DYYFE+IGEP+ +K +D+  D E+PPSQPLA+SE   ++FVAH
Sbjct: 2    SRVEIEEDTEGDRISTNDYYFERIGEPISIKEDDAQYDLENPPSQPLAISERHAVLFVAH 61

Query: 73   LSGFFVVRTEDVIASAKEMKNGGAGSSVQDLSIVDVSVGKVHILALSSDNSILAAVVAGD 132
             SGFFV RT DVI+++K     G    +QDLS+VDV VG V IL+LS+D+SILA  VA D
Sbjct: 62   SSGFFVGRTNDVISASKNSNGNGDKVFIQDLSLVDVPVGDVRILSLSADDSILAVTVAAD 121

Query: 133  VHLFSVGSLLDKAEKPSSSHSITDTSCIKDFKWTRKLENSYLVLSKHGELYQGSANGPPK 192
            +H FSV SLL K  KPS S+S  ++  +KDF+W R  ++SYLVLS  G+L+ G  N PP+
Sbjct: 122  IHFFSVDSLLKKDAKPSFSYSPDESGFVKDFRWRRNDKHSYLVLSNTGKLFHGIDNAPPR 181

Query: 193  HVMHDIDAVECSVKGKFIAVAKKDTLAIFSYKFKERLSMSLLPSLGNGNTDTDFAVKGSV 252
            HVM  +DAVE S KG +IAVA+ ++L IFS KF E+  ++L      G++D D  VK   
Sbjct: 182  HVMDAVDAVEWSSKGSYIAVAQDNSLRIFSSKFNEKRCIALSFDSWIGDSDEDCFVK--- 241

Query: 253  SVSPSVSLSKNDICKMLKLEVDCIKWVRADCIIIGCFQVTATGDEEDYFVQVVRSKDGKI 312
                                VD I+WVR +CI++GCFQ+   G EE+Y VQV+RS DGKI
Sbjct: 242  --------------------VDSIRWVRNNCILLGCFQL-IEGREENYLVQVIRSPDGKI 301

Query: 313  TDVSSNKVLLSFHDIHSGFTHDILPVETGPCLFLSYLDKCKLAIVANRNNMDQHIVLLGW 372
            +D S+N V LSF D+      D++PV  GP L  SY+D+CKLA+ ANR ++D+HIVLL W
Sbjct: 302  SDGSTNLVALSFSDLFPCSMDDLVPVGVGPHLLFSYIDQCKLAVTANRKSIDEHIVLLDW 361

Query: 373  LL-EVENEVAIIDIERDTSLPRINLQVSDRKTMDVLSLLSLIREVVLGPGRRDVRLWSPN 432
               + ++ V+++DI+R+T LPRI LQ                                  
Sbjct: 362  SSGDDKSAVSVVDIDRETFLPRIGLQ---------------------------------- 421

Query: 433  PIEDFSCRLFFRSLLYPSCENGDDNLVMGLCIDRFSLPGKVKVQVGAEETREVSPYCILL 492
                               EN DDN VMGLCIDR S+ G V V+ G +E +E+ PY +L+
Sbjct: 422  -------------------ENNDDNTVMGLCIDRVSIEGTVNVRSGDDELKELQPYFVLV 481

Query: 493  CLTLEGKLIMFHFSSVNESEAPHDVSACDEEEEDGTVEP--SDDQSQLSSESKNEFREAI 552
            CLTLEGKL+MF+ +SV    A  D       + +    P   DD S+ SSE   +   A+
Sbjct: 482  CLTLEGKLVMFNVASVAGRPASSDTDLASSSDIEDAYTPLIEDDLSKQSSEKHQQLNIAV 541

Query: 553  VS-LKMQDTEKIATNSEIPKEKINISSDIKPSNIDQSSVSNIDESVIVSGESYTKSQK-A 612
             +  K  +TEK +T   +P E           NI      ++  S  VSG++  K +  A
Sbjct: 542  QNDQKHLNTEKFSTEQRLPNE-----------NIFSKEFESVKSS--VSGDNNKKQEPYA 601

Query: 613  DSFIYSQSLRSSNLERPNNDTGNFDKPVQKFTGLGSVAFSGQSANVPSQSLKSSILERPN 672
            +  +  +  + S + R                       SG S      SL         
Sbjct: 602  EKPLQVEDAQQSMIPR----------------------LSGTSFGQLPMSL--------- 661

Query: 673  NEIGNFDKPVQKFTGLGSVAFSGQSANVPSQSLKSSILERPNNEIGNFDKPVQKFTGFGS 732
                 +D    KF G G        A   S+ L+  I  + N+        +Q      +
Sbjct: 662  ----GYD--TNKFAGFG-------PALPVSEKLQKDIFAQSNS------MHLQ-----AN 721

Query: 733  VTFSGQSAAMPSQSLKSSILERPNNEIGNCDKPVQKFTGLGSVAFSGQSVDVP---SQPF 792
            V     +A   S  L+++IL+ P N      +P            SG+SV  P   S PF
Sbjct: 722  VESKSTAAFFGSPGLQNAILQSPQN---TSSQPWS----------SGKSVSPPDFVSGPF 781

Query: 793  LNVKESTKRLGSTGLQAASELSSDKPMLFKKVDPVSSVLPLNSLQSSKTENYGPSFGAAN 852
             +++++  +     +Q+ +    + PM  K      SV  + + + S   N  P  G   
Sbjct: 782  PSMRDTQHK---QSVQSGTGY-VNPPMSIKD----KSVQVIETGRVSALSNLSPLLG--- 841

Query: 853  AFTGFAGKPFQQKDVPSTLTQSERQVTAGSGKIESLPVIRTSQTSLQDNFSTGKTANEKQ 912
                                   +    G  KIE +P IR SQ S Q   S  K+A+ +Q
Sbjct: 842  ---------------------QNQDTNEGVEKIEPIPSIRASQLSQQVKSSFEKSASHQQ 901

Query: 913  DGS---------DRNYSNVPLAKPMKEMCEGLDKLLESIEEPGGFLDACTAFQNSSVEAL 972
              +         + N SN P    + EM   +D LL+SIE PGGF D+C     S+VE L
Sbjct: 902  HKTPLSTGPLRLEHNMSNQP--SNINEMAREMDTLLQSIEGPGGFKDSCAFILKSNVEEL 961

Query: 973  ELGLATLSDQCQIWRRTMNKCAQEVPNLFDKTVQVLQKKTYIEGIVTQASDSNYWEHWDR 1032
            E GL +L+ +CQ W+ T+++   E+ +L DKT+QVL KKTY+EG+  Q +D+ YW+ W+R
Sbjct: 962  EQGLESLAGKCQTWKSTIHEQQAEIQHLLDKTIQVLAKKTYMEGMYKQTADNQYWQLWNR 1021

Query: 1033 QKLSSELELKRQHILKMNQNITNQLIELERHFNGLELNKFGGNDESQVSERALQRKFGST 1092
            QKL+ ELE KRQHI+K+N+++T+QLIELER+FN LEL+++  +    V+ R +  +   +
Sbjct: 1022 QKLNPELEAKRQHIMKLNKDLTHQLIELERYFNRLELDRYNEDGGHPVARRGVPNRSAPS 1081

Query: 1093 RHSHSLHSLNNIMGSQLAAAQLLSENLSKQMAALNIESPSLKRQSVTKELFETIGITYDA 1152
            R   SLHSL+N M SQLAAA+ LSE LSKQM  L I+SP   +++V +ELFETIGI YDA
Sbjct: 1082 RRVQSLHSLHNTMSSQLAAAEQLSECLSKQMTYLKIDSP--VKKNVKQELFETIGIPYDA 1141

Query: 1153 SFSSPNVNKIAETSTSKKLLLSADSFSSKDTSRRKQQSGTKNSEAETGRRRRDSLDRRRE 1212
            SFSSP+  K    S++K LLLS+   S    SR++Q S  KNS+ ET RRRR+SLDR   
Sbjct: 1142 SFSSPDAVKAKNASSAKNLLLSSIPASINQQSRQRQSSAMKNSDPETARRRRESLDRN-- 1201

Query: 1213 TTVGGAVRPDLGSGDGESTVRGGGARAGIRAARFVPLQGLDGGEVSRRQGEEGERRAAEE 1272
                 A  P       ++TV+                                 R   +E
Sbjct: 1202 ---WAAFEPP------KTTVK---------------------------------RMLLQE 1261

Query: 1273 GERRAANGRRATSCERAASDERTERARRRRNAEGHIRESHVQNALSYLQELLLYYYGFSV 1332
             ++   N +   S          ER R   N +      HV++  S              
Sbjct: 1262 QQKTGMNQQTVLS----------ERLRSANNTQDR-SLLHVKDHAS-------------- 1321

Query: 1333 LIRHVYELEIGFMEMAKNLASVEPPKTTVKRMLLQGIPLSGEKQFRSRTPEGVAAVARPA 1392
                V     G ME  +   S             Q  P      F++R P     + +  
Sbjct: 1322 ---PVVSSNKGIMESFQQDTSE-----------AQSTP------FKTRPP-----MPQSN 1381

Query: 1393 SCITSSMLSSS--SKNAENGSENPATPFTWASPPQPSNSSRQKSQPLQKANATTPSPLPV 1452
            S  T S +S+S  S N      +  T +   S P     +R  SQP     ++     PV
Sbjct: 1382 SPFTISPISASKPSFNWSGNKSSNTTSYAEESAPSQIKDTRTVSQP---GGSSFLPKRPV 1441

Query: 1453 FQSSHEMLKKGTNE-AYSVTSENKFAEVT------FPEKSKSSDFFS------------- 1512
              +  E  +K   E  +S    N F E            S  SDF S             
Sbjct: 1442 ASTVLEQTEKKAGEFKFSEAKANAFVETAAGSVQRLSTTSSGSDFESSKGFGAQFSTMSS 1501

Query: 1513 -LTRNDSVQKPNMNLDQKSSI----FTIPAKQTP---TPKDSIDT-SNSNSQKTANVKER 1572
                +    K     +  SSI    FT PA   P   TP DS  T   ++S   ++  + 
Sbjct: 1502 GAPASSFSSKSLFGFNSSSSIPGDKFTFPAVTAPLSGTPLDSTSTLFTASSAPVSSSSQD 1561

Query: 1573 HTTTSPLFGSANKPESAFVGTASSLVSTVD-----GARKTEEKKSTIAFSPSVPAPA--- 1632
                S    SA  P++ F  T++S VS        G   T  K      +PS P+P+   
Sbjct: 1562 PVPASIPISSAPVPQT-FSVTSTSTVSATGFNVPFGKPLTSVKVDLNQAAPSTPSPSPGP 1621

Query: 1633 ----LFN----TPSSASTLFSGFPVSKSLPSSAAVIDLNKPGSTSTQLIFSSPVVSVSDS 1692
                 FN    +PSS   + S    S   P SA    ++   +++T  +  S  +  S S
Sbjct: 1622 TAGFTFNLPALSPSSPEMVSSSTGQSSLFPPSAPTSQVSSDQASATSSLTDSSRLFSSTS 1681

Query: 1693 L-----------FQAPKMVSPSPTLSSLNPTLDSSKKEL---PVPKSDTDTEKQASASKP 1752
            L           FQ+P++ +PS  +    P  +  K E     +  + +  +  A+A+K 
Sbjct: 1682 LSSTPPITPPDAFQSPQVSTPSSAVPITEPVSEPKKPEAQSSSILSTQSTVDSVANATKT 1741

Query: 1753 ESHELKLQ-------PPVTPADKNHVEP--TSGTQMVSKDVGGHVPNVIGDAQPQQPSAA 1812
            ++  L ++         VTP   +      +SGTQ     +     +  G +QPQQ S+ 
Sbjct: 1742 QNEPLPVKSEISNPGTTVTPVSSSGFLSGFSSGTQSSLASMAAPSFSWPGSSQPQQLSST 1801

Query: 1813 FVPLSAPNLTSKISANGKNETSDAVVTQDDDMDEEAPETNNNVEFSLSALGGFG-SSSPI 1872
              P  A   +S  SA+   E  D V TQ+D+MDEEAPE +   E S+ + GGFG  S+P 
Sbjct: 1802 PAPFPA---SSPTSASPFGEKKDIVDTQEDEMDEEAPEASQTTELSMGSFGGFGLGSTPN 1816

Query: 1873 SSAPKPNPFGGPFGNVNATSMNSSFTMAPPQSGELFRPASFSFQSPLASQAASQPTNSVA 1932
              APK NPFGGPFGN   T+ N  F M  P SGELF+PASF+FQ+P  SQ A        
Sbjct: 1862 PGAPKTNPFGGPFGNATTTTSN-PFNMTVP-SGELFKPASFNFQNPQPSQPA-------- 1816

Query: 1933 FSGGFGSGMAT--QAPSQGGFGQPAQIGVGQQALGNVLGSFGQSRQLGPSLPGTGSGSPG 1992
               GFGS   T  Q P+Q GFGQP+QIG GQQALG+VLGSFGQSRQ+G  LPG   GSP 
Sbjct: 1922 ---GFGSFSVTPSQTPAQSGFGQPSQIGGGQQALGSVLGSFGQSRQIGAGLPGATFGSPT 1816

Query: 1993 GF-------------------------SGGFTSVKPVGGGFAGVGTGGGGGFAGVGSGGG 2011
            GF                         +GGF ++   G GFAG  +   GGFA + SG G
Sbjct: 1982 GFGGSNPGSGLPNAPASGGFAAAGSSATGGFAAMASAGRGFAGASSTPTGGFAALASGSG 1816

BLAST of Spg009835 vs. TAIR 10
Match: AT1G55540.2 (Nuclear pore complex protein )

HSP 1 Score: 773.5 bits (1996), Expect = 4.2e-223
Identity = 737/2123 (34.72%), Postives = 1019/2123 (48.00%), Query Frame = 0

Query: 13   TQIPLEDGDEGEHVQTTDYYFEKIGEPVPVKLNDSIIDPESPPSQPLAVSESFGLIFVAH 72
            +++ +E+  EG+ + T DYYFE+IGEP+ +K +D+  D E+PPSQPLA+SE   ++FVAH
Sbjct: 2    SRVEIEEDTEGDRISTNDYYFERIGEPISIKEDDAQYDLENPPSQPLAISERHAVLFVAH 61

Query: 73   LSGFFVVRTEDVIASAKEMKNGGAGSSVQDLSIVDVSVGKVHILALSSDNSILAAVVAGD 132
             SGFFV RT DVI+++K     G    +QDLS+VDV VG V IL+LS+D+SILA  VA D
Sbjct: 62   SSGFFVGRTNDVISASKNSNGNGDKVFIQDLSLVDVPVGDVRILSLSADDSILAVTVAAD 121

Query: 133  VHLFSVGSLLDKAEKPSSSHSITDTSCIKDFKWTRKLENSYLVLSKHGELYQGSANGPPK 192
            +H FSV SLL K  KPS S+S  ++  +KDF+W R  ++SYLVLS  G+L+ G  N PP+
Sbjct: 122  IHFFSVDSLLKKDAKPSFSYSPDESGFVKDFRWRRNDKHSYLVLSNTGKLFHGIDNAPPR 181

Query: 193  HVMHDIDAVECSVKGKFIAVAKKDTLAIFSYKFKERLSMSLLPSLGNGNTDTDFAVKGSV 252
            HVM  +DAVE S KG +IAVA+ ++L IFS KF E+  ++L      G++D D  VK   
Sbjct: 182  HVMDAVDAVEWSSKGSYIAVAQDNSLRIFSSKFNEKRCIALSFDSWIGDSDEDCFVK--- 241

Query: 253  SVSPSVSLSKNDICKMLKLEVDCIKWVRADCIIIGCFQVTATGDEEDYFVQVVRSKDGKI 312
                                VD I+WVR +CI++GCFQ+   G EE+Y VQV+RS DGKI
Sbjct: 242  --------------------VDSIRWVRNNCILLGCFQL-IEGREENYLVQVIRSPDGKI 301

Query: 313  TDVSSNKVLLSFHDIHSGFTHDILPVETGPCLFLSYLDKCKLAIVANRNNMDQHIVLLGW 372
            +D S+N V LSF D+      D++PV  GP L  SY+D+CKLA+ ANR ++D+HIVLL W
Sbjct: 302  SDGSTNLVALSFSDLFPCSMDDLVPVGVGPHLLFSYIDQCKLAVTANRKSIDEHIVLLDW 361

Query: 373  LL-EVENEVAIIDIERDTSLPRINLQVSDRKTMDVLSLLSLIREVVLGPGRRDVRLWSPN 432
               + ++ V+++DI+R+T LPRI LQ                                  
Sbjct: 362  SSGDDKSAVSVVDIDRETFLPRIGLQ---------------------------------- 421

Query: 433  PIEDFSCRLFFRSLLYPSCENGDDNLVMGLCIDRFSLPGKVKVQVGAEETREVSPYCILL 492
                               EN DDN VMGLCIDR S+ G V V+ G +E +E+ PY +L+
Sbjct: 422  -------------------ENNDDNTVMGLCIDRVSIEGTVNVRSGDDELKELQPYFVLV 481

Query: 493  CLTLEGKLIMFHFSSVNESEAPHDVSACDEEEEDGTVEP--SDDQSQLSSESKNEFREAI 552
            CLTLEGKL+MF+ +SV    A  D       + +    P   DD S+ SSE   +   A+
Sbjct: 482  CLTLEGKLVMFNVASVAGRPASSDTDLASSSDIEDAYTPLIEDDLSKQSSEKHQQLNIAV 541

Query: 553  VS-LKMQDTEKIATNSEIPKEKINISSDIKPSNIDQSSVSNIDESVIVSGESYTKSQK-A 612
             +  K  +TEK +T   +P E           NI      ++  S  VSG++  K +  A
Sbjct: 542  QNDQKHLNTEKFSTEQRLPNE-----------NIFSKEFESVKSS--VSGDNNKKQEPYA 601

Query: 613  DSFIYSQSLRSSNLERPNNDTGNFDKPVQKFTGLGSVAFSGQSANVPSQSLKSSILERPN 672
            +  +  +  + S + R                       SG S      SL         
Sbjct: 602  EKPLQVEDAQQSMIPR----------------------LSGTSFGQLPMSL--------- 661

Query: 673  NEIGNFDKPVQKFTGLGSVAFSGQSANVPSQSLKSSILERPNNEIGNFDKPVQKFTGFGS 732
                 +D    KF G G        A   S+ L+  I  + N+        +Q      +
Sbjct: 662  ----GYD--TNKFAGFG-------PALPVSEKLQKDIFAQSNS------MHLQ-----AN 721

Query: 733  VTFSGQSAAMPSQSLKSSILERPNNEIGNCDKPVQKFTGLGSVAFSGQSVDVP---SQPF 792
            V     +A   S  L+++IL+ P N      +P            SG+SV  P   S PF
Sbjct: 722  VESKSTAAFFGSPGLQNAILQSPQN---TSSQPWS----------SGKSVSPPDFVSGPF 781

Query: 793  LNVKESTKRLGSTGLQAASELSSDKPMLFKKVDPVSSVLPLNSLQSSKTENYGPSFGAAN 852
             +++++  +     +Q+ +    + PM  K      SV  + + + S   N  P  G   
Sbjct: 782  PSMRDTQHK---QSVQSGTGY-VNPPMSIKD----KSVQVIETGRVSALSNLSPLLG--- 841

Query: 853  AFTGFAGKPFQQKDVPSTLTQSERQVTAGSGKIESLPVIRTSQTSLQDNFSTGKTANEKQ 912
                                   +    G  KIE +P IR SQ S Q   S  K+A+ +Q
Sbjct: 842  ---------------------QNQDTNEGVEKIEPIPSIRASQLSQQVKSSFEKSASHQQ 901

Query: 913  DGS---------DRNYSNVPLAKPMKEMCEGLDKLLESIEEPGGFLDACTAFQNSSVEAL 972
              +         + N SN P    + EM   +D LL+SIE PGGF D+C     S+VE L
Sbjct: 902  HKTPLSTGPLRLEHNMSNQP--SNINEMAREMDTLLQSIEGPGGFKDSCAFILKSNVEEL 961

Query: 973  ELGLATLSDQCQIWRRTMNKCAQEVPNLFDKTVQVLQKKTYIEGIVTQASDSNYWEHWDR 1032
            E GL +L+ +CQ W+ T+++   E+ +L DKT+QVL KKTY+EG+  Q +D+ YW+ W+R
Sbjct: 962  EQGLESLAGKCQTWKSTIHEQQAEIQHLLDKTIQVLAKKTYMEGMYKQTADNQYWQLWNR 1021

Query: 1033 QKLSSELELKRQHILKMNQNITNQLIELERHFNGLELNKFGGNDESQVSERALQRKFGST 1092
            QKL+ ELE KRQHI+K+N+++T+QLIELER+FN LEL+++  +    V+ R +  +   +
Sbjct: 1022 QKLNPELEAKRQHIMKLNKDLTHQLIELERYFNRLELDRYNEDGGHPVARRGVPNRSAPS 1081

Query: 1093 RHSHSLHSLNNIMGSQLAAAQLLSENLSKQMAALNIESPSLKRQSVTKELFETIGITYDA 1152
            R   SLHSL+N M SQLAAA+ LSE LSKQM  L I+SP   +++V +ELFETIGI YDA
Sbjct: 1082 RRVQSLHSLHNTMSSQLAAAEQLSECLSKQMTYLKIDSP--VKKNVKQELFETIGIPYDA 1141

Query: 1153 SFSSPNVNKIAETSTSKKLLLSADSFSSKDTSRRKQQSGTKNSEAETGRRRRDSLDRRRE 1212
            SFSSP+  K    S++K LLLS+   S    SR++Q S  KNS+ ET RRRR+SLD    
Sbjct: 1142 SFSSPDAVKAKNASSAKNLLLSSIPASINQQSRQRQSSAMKNSDPETARRRRESLD---- 1201

Query: 1213 TTVGGAVRPDLGSGDGESTVRGGGARAGIRAARFVPLQGLDGGEVSRRQGEEGERRAAEE 1272
                                     R     A F P +               +R   +E
Sbjct: 1202 -------------------------RVIFNWAAFEPPK------------TTVKRMLLQE 1261

Query: 1273 GERRAANGRRATSCERAASDERTERARRRRNAEGHIRESHVQNALSYLQELLLYYYGFSV 1332
             ++   N +   S          ER R   N +      HV++  S              
Sbjct: 1262 QQKTGMNQQTVLS----------ERLRSANNTQDR-SLLHVKDHAS-------------- 1321

Query: 1333 LIRHVYELEIGFMEMAKNLASVEPPKTTVKRMLLQGIPLSGEKQFRSRTPEGVAAVARPA 1392
                V     G ME  +   S             Q  P      F++R P     + +  
Sbjct: 1322 ---PVVSSNKGIMESFQQDTSE-----------AQSTP------FKTRPP-----MPQSN 1381

Query: 1393 SCITSSMLSSS--SKNAENGSENPATPFTWASPPQPSNSSRQKSQPLQKANATTPSPLPV 1452
            S  T S +S+S  S N      +  T +   S P     +R  SQP     ++     PV
Sbjct: 1382 SPFTISPISASKPSFNWSGNKSSNTTSYAEESAPSQIKDTRTVSQP---GGSSFLPKRPV 1441

Query: 1453 FQSSHEMLKKGTNE-AYSVTSENKFAEVT------FPEKSKSSDFFS------------- 1512
              +  E  +K   E  +S    N F E            S  SDF S             
Sbjct: 1442 ASTVLEQTEKKAGEFKFSEAKANAFVETAAGSVQRLSTTSSGSDFESSKGFGAQFSTMSS 1501

Query: 1513 -LTRNDSVQKPNMNLDQKSSI----FTIPAKQTP---TPKDSIDT-SNSNSQKTANVKER 1572
                +    K     +  SSI    FT PA   P   TP DS  T   ++S   ++  + 
Sbjct: 1502 GAPASSFSSKSLFGFNSSSSIPGDKFTFPAVTAPLSGTPLDSTSTLFTASSAPVSSSSQD 1561

Query: 1573 HTTTSPLFGSANKPESAFVGTASSLVSTVD-----GARKTEEKKSTIAFSPSVPAPA--- 1632
                S    SA  P++ F  T++S VS        G   T  K      +PS P+P+   
Sbjct: 1562 PVPASIPISSAPVPQT-FSVTSTSTVSATGFNVPFGKPLTSVKVDLNQAAPSTPSPSPGP 1621

Query: 1633 ----LFN----TPSSASTLFSGFPVSKSLPSSAAVIDLNKPGSTSTQLIFSSPVVSVSDS 1692
                 FN    +PSS   + S    S   P SA    ++   +++T  +  S  +  S S
Sbjct: 1622 TAGFTFNLPALSPSSPEMVSSSTGQSSLFPPSAPTSQVSSDQASATSSLTDSSRLFSSTS 1681

Query: 1693 L-----------FQAPKMVSPSPTLSSLNPTLDSSKKEL---PVPKSDTDTEKQASASKP 1752
            L           FQ+P++ +PS  +    P  +  K E     +  + +  +  A+A+K 
Sbjct: 1682 LSSTPPITPPDAFQSPQVSTPSSAVPITEPVSEPKKPEAQSSSILSTQSTVDSVANATKT 1741

Query: 1753 ESHELKLQ-------PPVTPADKNHVEP--TSGTQMVSKDVGGHVPNVIGDAQPQQPSAA 1812
            ++  L ++         VTP   +      +SGTQ     +     +  G +QPQQ S+ 
Sbjct: 1742 QNEPLPVKSEISNPGTTVTPVSSSGFLSGFSSGTQSSLASMAAPSFSWPGSSQPQQLSST 1801

Query: 1813 FVPLSAPNLTSKISANGKNETSDAVVTQDDDMDEEAPETNNNVEFSLSALGGFG-SSSPI 1872
              P  A   +S  SA+   E  D V TQ+D+MDEEAPE +   E S+ + GGFG  S+P 
Sbjct: 1802 PAPFPA---SSPTSASPFGEKKDIVDTQEDEMDEEAPEASQTTELSMGSFGGFGLGSTPN 1819

Query: 1873 SSAPKPNPFGGPFGNVNATSMNSSFTMAPPQSGELFRPASFSFQSPLASQAASQPTNSVA 1932
              APK NPFGGPFGN   T+ N  F M  P SGELF+PASF+FQ+P  SQ A        
Sbjct: 1862 PGAPKTNPFGGPFGNATTTTSN-PFNMTVP-SGELFKPASFNFQNPQPSQPA-------- 1819

Query: 1933 FSGGFGSGMAT--QAPSQGGFGQPAQIGVGQQALGNVLGSFGQSRQLGPSLPGTGSGSPG 1992
               GFGS   T  Q P+Q GFGQP+QIG GQQALG+VLGSFGQSRQ+G  LPG   GSP 
Sbjct: 1922 ---GFGSFSVTPSQTPAQSGFGQPSQIGGGQQALGSVLGSFGQSRQIGAGLPGATFGSPT 1819

Query: 1993 GF-------------------------SGGFTSVKPVGGGFAGVGTGGGGGFAGVGSGGG 2011
            GF                         +GGF ++   G GFAG  +   GGFA + SG G
Sbjct: 1982 GFGGSNPGSGLPNAPASGGFAAAGSSATGGFAAMASAGRGFAGASSTPTGGFAALASGSG 1819