Spg009799 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg009799
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionLactase
Locationscaffold7: 6568023 .. 6576799 (+)
RNA-Seq ExpressionSpg009799
SyntenySpg009799
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AATTCAATTTCTGTTCTGGGTCTGCTCGGATTTCATGGTTTAATTTTGAAACCAGTGCTGTGGCTGCAGGTCATGGCTTCATTGGCTGCTAAGCTTATGATTCCCTCTGAGAATGGTTACAGGGTGTGGGAAGACCAAAATTTCATCAAGTGGAAGAAGAGGGATTCTCACGTTCCTCTACGCTGCCAGGATTCTGTTGAAGGTATTCGTTTTTAGTTTGTTTCTTTTTTACGGATGTGGTTTCGATCAAATGCTCGTTTTTTCTCTTGTGGGTCGAGGAAATTAGTTGGTTTCCTCTGTCTTGGATTTTGACATTGTCTTCTTCATTGTTTAAGCACCGTCATTAGTGCTTAATAATGTACATTGTGTGCATATCTTTGTGTTGATTAGTGAAATTAGGAAGCTCTAGAAAGAGTGTGACTAATATCAGTGCCCTGGTATGGAGTTGGGACGTTTGAAGGTACCTTTGATAGGCGACTTAATAACAACCCTTGTTGGCTCTCCGAGTTGGCATTGCGGTGGGCAAAGCCTCTTGGGGATTATTGGGTGGGACATGAGCATAATTGAAGGAGTGGAGCCCCGGATACCATAGTGTGCGGAAAGAATGTGATTAACATTGCTTTTAGAATGCACTTGTGATGAATTTATATGGTTATATCTCTGCATGTTTTACTTCTTATGGTTTGACCACCTCTTTATAGCTCAAAGGCTTAAATCAATGAAATGCTTTTAATCATTTTGATGTAAGCAGTCTGCCAGTGAGAGGGAACGGTTTTGGGAGTAACATTTGAGATTATCTAATGAAGCAAACTATTAGTAAACTTATTTGAGGAAACTCTGGTGTTTTGTGTATGCATAGGATGCCTCAAGTACTGGCATGACCGTAGTAAAGTAGATTTTCTGGTGTCGAACTCTGCTGTTTGGAATGATGATGCTGTTCAAAGTGCTCTTGACTGTGCTGCTTTCTGGGTTAAGGGCTTACCTTTCATCAAGTCTTTATCTGGTTATTGGAAATTTTATTTGGCCGCTACTCCAACGAGCGTTCCAGAGAATTTTCATGGAAGTGCATTTGAGGATTCTGAATGGACAAATTTGCCAGGTATATTTCAATGCTCAGAAGATACAGCTTGACATACATGAATTAGGTGGTAGATAGTTATCCATTCTTTTGTAACATCTTCACATTTTCAAAATTTTCATCTGTATTAATTATGTTAAAATGTGTTTTTCATGCTTTTTTGCATTAGGCGCTTTATTTCTAACAAACTGAATTAGCCATCTTCATAAGCATGTAAGATGTTTGGAGATACCCTTCAAGGTCCTTGGTTCAGGCCTGGTTGGTGGATTGGTTTTTGAAAATCAAATTATTAGAGTTCAAAAAAGTCAAAACATAACTTTATTGTTGCATATATGAATCTTTAGGACGACAAGAAGGAGTGGAGAACATCTGTTTTTCTTCTTCCACATTGTCTGATGAACACATATATTTTGTCTGATAAATTTTTTATTGCTCGACTATTTGGAACAGTTCCTTCAAATTGGCAGATGCATGGGTTTGATCGGCCCATTTATACCAATGTTGTTTATCCCTTCCCTCTCGATCCTCCGCATGTTCCTGAGGACAATCCTACTGGCTGCTACAGAACTTATTTTCAACTTCCAGAAGAATGGAAAGGTTCTTTTTCTTTCTGTTTTTCTTTTTTCCTGTTCTCTAAAAAGATAAGAAACAATACCTATTAACATTAATCAATATTCTCTCTTTTAAAACCTATATTAGATTTGATGCAAAAACAGTTTGTATTTGACAGGTCGTAGAATTTTGTTGCACTTTGAAGCTGTTGATTGTGCATTTTTTGTGTGGATAAATGGGAGTCTTGTTGGGTACAGGTAAACAGTGACGTTTTAGGAAAATTTATTTGTCATATTGGTGTTGTCTTTTATAGTATTGTTCGCTTTTGTGCTGTTTTTTTTACAAGAAAATAGAAGGAATAAGTACATTTTGTTTACCATTTTAGCTATTGAAATGTGCTTCGACTTGCGGTTTTTAGTAGACTACGCCAAATGAAGGTGTGGTCATTGGGAGGGGTTTGGTGAAATTAGTATTGCAAGCAACTTGGAAATCAGGAGATTGTACTGTTGGATATGAAACTGTGGTTAAAGCCTTTCATATGGGCTGTGAGAAATCTGAGATTTTTTTAAAAGTTAAATATTTGGTGTGGTTATGGTTAGGTCAGTCAGGACAGTAGACTACCAGCAGAATTTGAAATAACAGAATATTGCCATCCATGTGGCTCAAAGTCCAAGAATGTTCTTGCTGTTCAAGTGTTAAAATGGAGTGATGGTTCTTACCTTGAGGACCAAGACCAGTGGTGGCTCTCAGGAATTCATCGTGATGTCCTTCTCTTATCCAAACCTCAGGTATGTTTTTCAATATCAATCTTCTCTCTACTCTATTTTCAGTTTTATATGTCAAGTTTTTTTTTAATTAATTATGTTGGTTAAAAAAAATAAACTTCCAACTAAATCACTATAATTATTTATTGGTTTTTTAATATATATTTTAATTTAATTAAATAGTAATAATTTCTTTAAAACGATGAAGCAAAACTTAGATTACTGTGTTTCTAATACTTTGAAATTAAATAGTCTAGAAAATGAAGTTGAAATAATTTTGCTTTTGTTTCTAATTTGGTTACTTTTATTGTTGATTAGCATTGATGAGATGGAAATGACAAGATGGAGATATCGTAAATTAGAAATTGATTGGTGTCTGTTTCTTTGGTTTGTATGTAGGTATTCATAGGGGACTACTTTTTCAAATCATGTGTTGGGGAGGATTTTTCTTATGCTGATATACAGGTACGCTGTAGATTCACACCATTTATCTGCTTTCAGTATAAACTTTTGTCATTGTTTTTGAACTAAAAATAAATGTGATCATTTGAAGTTTCAACTTGTATTTTAAAATATCCAGATCGAAACTTTCAATTCCGCAAATGGTTGATGCTTCTGTTTTTCAAATGGTTATACACATATGTCATCGGAAAAGCATATGATTTATTGAAACAAGAGTGGTGATTCAATTAGAATTCTATGAAGTGATGATAGGAGTGAAATGAGTTTAATCTGTATGCATAGTGAAAGAAAAGTGAGAAGTTGGATATAGAGTTAGGACTCATATGGGAAGAAGTTCCAAGGAATGATGGATTGTCAGTTGGACAATAATAATAATAATAAAGGCAAATTGCACAAACCACCCCTAAACTGTAGTGGTTGCTACTATTGCACCTACAATGTTTTAATTGTAAAAGTTAGACCTTCAAACTTTCATAAGTGAAAAAATTGGATCCATAAACTTTGATTGTATGTATATATATAATATATTTATGAGAAACAAAATCATTATATTAGTGAGAAAAGGAGGAACAGCCTAAAGGCCTTAGGAAAGAGGGATCCCTGTAATGTGTATATTATATTTTAAATTGTTTTTTCTTGTTATCTTTTAAGCTGGATCAATATTAAAAATTTGATCTTCAATTGCAATACAATTGAAAACACTGATTCAAATAACTCTTAGAAAATAGAGCCATATCAGGGAGTAGTAGTGAATTCTCTCCTAAAACTTTTAATATAACCTGGCATGCATGATGTTTGTTTCTGTTCATTGTTCTGAATAATCGTGGATTTGTCATGCATATTTGCTTGTTCTCCCTCTCATACCCATGAACATATATACTTTAAAAATTAAAATGATTGTTTATTACTTAATTATATGGTTTATTACTTTTAATTAAACTTAGGTATTCATTGATTAACTTAAACAATCTCTATAAACATTTCTGGTATCATTTATTTTTCTCAATTTTCATTGTTTTATCTGTAAATCTGAATAATCAATGTTAGTATTTCCATCCTTCATTGCTTTGCTGCTAATGGGGCAATATCCATGAACTGGCGTATAGGTTTTGTTATCTTGATGGCATAAATTTCTCAAACTTTATGTTCATCTCAGCGTAGAATACTCATTACTTGACAGAAACTTGAAATGCATGTTTATTTTATCTTACAGGTTGAAGTGAAAATTGACAACTCTCTTGATACAAGCAAGGAAAATTTTCTTAACGACTTTAAGTTAGAAGCAATATTGTTTGACAATAGAACCTGGGACAATCATGAAGGCCAAGCCGATCTGCTCTCATCAAATGTGGCCAATGTTAAGCTTACTCTCCTCTCTGCGACAACTCTGGGGTTCCATGGTTATGTACTTGGGGGAAGATTGGAAAAGCCTAAGCTTTGGTCTGCAGAGCACGTAAGGAAGATTCTCATTAATCTGCTAGTTTCTCTTCTCTTTTTTTTGTCTCTGCATGCTAATGTTTAATCTTCTCTTTTCCCTCTTAAACAGCCACAGCTCTACACTCTTATTATTCTTCTGAAAGACTTGTCGGATCAGATTGTTGACTGTGAGTCATGCCTAGTTGGAATAAGAAGTATTACAAAAGCTCCAAAACAGCTACTTGTCAATGGATGTCCAGTAGTAATCAGAGGCGTGAATAGGCACGAGCATCATCCGCGTCTTGGGAAGACGAATATAGAGTCCTGCATGGTTCAGGTGACGCCTCCTCCTTTGTTTTTCTTTGGGGAAAAAATTGAACTTTCCAAACCCAACTACAAGAGTACTCAAATCTAACCAAAAAAATCGAGTAGATAAAAAACATTCAAACATAATTAGAAGAACGGGTGGGTACCACTAAACCCAACTTTTCCCATTTCTTAGACTAAAGAGGCATAATTTAGAGGCCATAGTGTTGGTACACTATATGGTGGGTGAGTTGTTTGTTTACTTTTGTCGTTTCAGGATTTGGTCCTAATGAAACAAAACAACATGAATGCGGTGAGAAACAGCCATTATCCTCAACACCCTCGTTGGTATGAACTGTGTGATTTGTTTGGCATGTACATGATAGACGAGGCCAACATTGAAACACATGGTTTTGATTATACTGGACATGTGAAGCATCCAACTTTGCAACCGAGTTGGGCTGCTGCAATGTTAGATCGTGTAATAGGAATGGTGGAAAGAGACAAGAATCATGCCTGTATTATAGCTTGGTCCTTGGGAAATGAGGCTGGATATGGGCCTAACCACTCAGCTCTAGCTGGTATGTCATTTTGATTTAATTATATCTTTAAGTTGGTTTTAGTTGAAAGAAATGCTTGAGATATACTAGTTTGGTTCTTGCAAGGCCTATTTATCTCGTGTATGCAATTTTTTTAATTTCTGCAAGAAAAATTTTGACCCCTACATATTGGTGTAGGGTGGATTCGTGGAAAAGATTCGTCCCGAGTGTTGCACTATGAAGGAGGAGGGTCTAGAACCTCTTCTACTGATATAATCTGTCCCATGTACATGCGTGTATGGGACATGGTGAAGATTGCTAATGATCCCAATGAAACTCGTCCTTTGATTCTCTGCGAGTAAGTCTTGTCAATAACTCTTTTTTAACTTTATGAGGATAGTTTTACAACATTTCCATTGTGGGTTTTTGTTAATGTTATACATCTTAAAAAACAATAGGTACTCGCACTCAATGGGAAACAGCACTGGGAATCTTCATAAATATTGGGAAGCAATTGACAACACCTTTGGCCTCCAAGGAGGGTTTATTTGGGATTGGGTTGACCAGGTTTGGGCACTTGTCTAACCCTATTGATAATCTAATGATATATAGTTACTATAAAAAGGAAAATCTATTGTTTGAGATCAATTGTTTATTAGTTCTAGCTATGAATTATCTGGTTCAATTTTCTAATTTTATTTTCTGCAAACTACTTTCTAATCATTCACTTAGATCATTAACCCTTTTTTGCATTAGTTTCAAAGGTTGTACTTTTTGATCTAGGCACTTCTGAAGGAGGTTGACAATGGCAGGAAGCGATGGGCATATGGAGGTGAATTTGGGGATGTCCCAAATGATTCAAATTTCTGTCTCAACGGTGTGACATGGCCAGATCGAACTCCGCATCCTGCACTGCATGGTAAATTGATAACATCTTATTATTTATATAATTACGTGATTAGCAACGATTTTGAATGCATCAAAAATGTTACTGACCAGTTTTCGTATTTGCGAAACTCAATTTTCAGAGGTCAAGTATCTTTATCAGCCAATCAAGATTTCATCAAAGGATGGGGCACTTCAGGTCTTTCAGATACCTAACTAGATTATTTCATCATTTATTGAACCTTGGAGGGGGAGATGTATGACATCGAATTTCCATTTTACAGGTTCTGAATGCTCATTTTTTTTCAACCACAGAGGATTTGGAGTTCAGCTGGAGCATTTATGGTGATGGTCTTGAACTTGGAAATGGAATTCTCTCTCTTCCTGTAATAGGTCCTCAGGAAAGTTATAATATTGAATGGCAAACAAGTCCATGGTATGATCTATGGGCTTCGTCACCTGCATTAGAGTTCTTTTTAAATGTATCAGTGAAACTTCTGCACTCAACACGATGGGTCGAAGCTGGTCACATCATTTCATCATCACAAGTCCAGTTGCCGATGAAACGAGAATTTTTTCCTCATGTAATTGCTTATAATTAATGCCAAGTCAAAGAATTTACTATTCCAATTATATAAATAACTGTATAAAAATTTGTGTTGATTTGCAGTCCATCAAGAATGGAAGTGCTAGTTTACTTAGTGAAATTCTTGGGGATACAGTTCGAGTCTACCAGCAGAACTTATGGGAGATTAAATTGGATGTTCAAACTGGAACACTCGAAAGCTGGAAGGTAATATAGTATATGATTGGATTTGTAGTATATTACTTTGGGGGGCTGATGTATCAGCCTAGGTACTATGACAAGTCTTTGGAGTTTTAAGAGAACTAAGGAGTCCTGATGTTGAGTGTTGACTTGTTGTAGGTTAAAGGAGTTCCTCTAATAATTAAAGGTATTGTACCTTCCTTCTGGCGAGCACCTACTGATAACGACAAGGGTGGAGGTTCATGTAGTTATTTATCCTTGTGGAAAGCTGCCCATATTGACAGCCTCTCTTTCACTGCCGAAAGATGTTCCATACTCAGTACGACAGAACATTATGTGAAAATCGCTGTCGTTTTTCTGGGTCTTGAGAATGATGAGCAGCAGGCTTCTTTCTCTGACTCAGAGAAATCAAATGTCTTAATTCAAGTGGATATGACTTATACAATTTATGGTTCTGGGGATGTGATTGTGGACTGCAATGTACAGCCAAGTCCAAATCTTCCTCCTTTGCCTCGTGTGGGAGTCGAATTTCATCTAGATAAATCTGTGGACCAGGTCAGATGGTATGGAAGAGGGCCGTTTGAGTGCTATCCCGATCGAAAAGCAGCGGCTCATGTTGGAGTTTATGAGAAGAATGTGTCTGAAATGCATGTCCCGTACATTGTCCCTGGAGAATCTTCAGGTAGGGCTGATGTTAGGTGGGTTACTTTTGAAAACAAGGATGGTGTTGGAATCTATGCCTCAATATACAGAGGCTCACCGCCTATGCAAATGAGTGCAAGCTATTACTCCACGGAAGAGCTCGACCGGGCAGTACATAATGAGGACCTTGTTGAGGGAGACGACATTGAGGTAGTTATTTCAGTTGTCTGTATATCTAACATCTGAGCAATTAACCCACGTTATACGTCGTTGAATCGACTCGTAGATATTTTCTAATGTTGATTCTTCTGTTGGCTTTTTACTCATCCTCTACATTCCTAGTTCTTAGTTTCATCGTGGTGTCATGTAGGTTCATCTTGACCACAAGCACATGGGTGTGGGAGGAGACGACAGCTGGTCTCCCTGTGTACATGATGAGTATTTGCTTTCTCCGGTGCCATACTCGTTCTCGATCCGGTTCTATCCGTTAACTCCATCTGCTTCTGGCTATGATGCTTACAAATCCCAACTCCCATTGTAGTGAATATGGCATCAGCATTCAACCAAGTAAAGAACTTGTAATAATCCTTTGGTTTGCTTAATGACAAAAGGGACATGCCAGATTGCATTCTTTTTCCTTCTCCCTGCTTCTCTGTAGATGTAATATTCAGTAGAATCTCACTCGGCCATGGCGCGGAGACGAGGGATCGATTCAAGTTCTCAGTTGTCTATAGGGTTATATGAACAAGAATCACAATAATGGCAATGTTGCTATGAGTTCAGGTCAAAGTGAATTTGTATTGATCTTATTGAGACAATCCATTTCCTTTTGTGTGCTGTTTCCTTCTCATGTTTGCAACAGTAGTATCTGTCTTGTATGCTTGATGTGGAATAATACATTGATGTTAGAGATGATTTTGATTACTGGGCTCTGGAAAGAAGTTGGGGAGTTTTTTTTTTTTTTTTTTTTTGAAAAGTAGAAGTTGGGGAGTTTGAACTGCTAAAGTATAGTTTTTTTTTTTTTTAATTTATTTATTTTAATCCGAGGATAATTTAGGTTGCGTTTGGTAGGTGATCTGAAAATAGAAAATTGAAAACAAGGGATTCATGAAAACAGAGTTGTATTTCATGTTTTCGAATTTGAATACTTGAATGTGTATTAATTAACATGGTTGACAAGACATGTATTTATAGGGAGAGTTGAAACAAAGAGATAAAAAATTGAACTTCCAACCTTTTTAAGTGAGGTTTATGATGCATTAATTTTGTCCATGTGTTGCTTAATGGGACATATATCAAAGTCATATAAGTTGGTTGACGTATTAGATGGATAAATGTTAGATTGCATAGTAGCAAGACTAAGAACC

mRNA sequence

AATTCAATTTCTGTTCTGGGTCTGCTCGGATTTCATGGTTTAATTTTGAAACCAGTGCTGTGGCTGCAGGTCATGGCTTCATTGGCTGCTAAGCTTATGATTCCCTCTGAGAATGGTTACAGGGTGTGGGAAGACCAAAATTTCATCAAGTGGAAGAAGAGGGATTCTCACGTTCCTCTACGCTGCCAGGATTCTGTTGAAGGATGCCTCAAGTACTGGCATGACCGTAGTAAAGTAGATTTTCTGGTGTCGAACTCTGCTGTTTGGAATGATGATGCTGTTCAAAGTGCTCTTGACTGTGCTGCTTTCTGGGTTAAGGGCTTACCTTTCATCAAGTCTTTATCTGGTTATTGGAAATTTTATTTGGCCGCTACTCCAACGAGCGTTCCAGAGAATTTTCATGGAAGTGCATTTGAGGATTCTGAATGGACAAATTTGCCAGTTCCTTCAAATTGGCAGATGCATGGGTTTGATCGGCCCATTTATACCAATGTTGTTTATCCCTTCCCTCTCGATCCTCCGCATGTTCCTGAGGACAATCCTACTGGCTGCTACAGAACTTATTTTCAACTTCCAGAAGAATGGAAAGGTCGTAGAATTTTGTTGCACTTTGAAGCTGTTGATTGTGCATTTTTTGTGTGGATAAATGGGAGTCTTGTTGGGTACAGTCAGGACAGTAGACTACCAGCAGAATTTGAAATAACAGAATATTGCCATCCATGTGGCTCAAAGTCCAAGAATGTTCTTGCTGTTCAAGTGTTAAAATGGAGTGATGGTTCTTACCTTGAGGACCAAGACCAGTGGTGGCTCTCAGGAATTCATCGTGATGTCCTTCTCTTATCCAAACCTCAGGTATTCATAGGGGACTACTTTTTCAAATCATGTGTTGGGGAGGATTTTTCTTATGCTGATATACAGGTTGAAGTGAAAATTGACAACTCTCTTGATACAAGCAAGGAAAATTTTCTTAACGACTTTAAGTTAGAAGCAATATTGTTTGACAATAGAACCTGGGACAATCATGAAGGCCAAGCCGATCTGCTCTCATCAAATGTGGCCAATGTTAAGCTTACTCTCCTCTCTGCGACAACTCTGGGGTTCCATGGTTATGTACTTGGGGGAAGATTGGAAAAGCCTAAGCTTTGGTCTGCAGAGCACCCACAGCTCTACACTCTTATTATTCTTCTGAAAGACTTGTCGGATCAGATTGTTGACTGTGAGTCATGCCTAGTTGGAATAAGAAGTATTACAAAAGCTCCAAAACAGCTACTTGTCAATGGATGTCCAGTAGTAATCAGAGGCGTGAATAGGCACGAGCATCATCCGCGTCTTGGGAAGACGAATATAGAGTCCTGCATGGTTCAGGATTTGGTCCTAATGAAACAAAACAACATGAATGCGGTGAGAAACAGCCATTATCCTCAACACCCTCGTTGGTATGAACTGTGTGATTTGTTTGGCATGTACATGATAGACGAGGCCAACATTGAAACACATGGTTTTGATTATACTGGACATGTGAAGCATCCAACTTTGCAACCGAGTTGGGCTGCTGCAATGTTAGATCGTGTAATAGGAATGGTGGAAAGAGACAAGAATCATGCCTGTATTATAGCTTGGTCCTTGGGAAATGAGGCTGGATATGGGCCTAACCACTCAGCTCTAGCTGGGTGGATTCGTGGAAAAGATTCGTCCCGAGTGTTGCACTATGAAGGAGGAGGGTCTAGAACCTCTTCTACTGATATAATCTGTCCCATGTACATGCGTGTATGGGACATGGTGAAGATTGCTAATGATCCCAATGAAACTCGTCCTTTGATTCTCTGCGAGTACTCGCACTCAATGGGAAACAGCACTGGGAATCTTCATAAATATTGGGAAGCAATTGACAACACCTTTGGCCTCCAAGGAGGGTTTATTTGGGATTGGGTTGACCAGGCACTTCTGAAGGAGGTTGACAATGGCAGGAAGCGATGGGCATATGGAGGTGAATTTGGGGATGTCCCAAATGATTCAAATTTCTGTCTCAACGGTGTGACATGGCCAGATCGAACTCCGCATCCTGCACTGCATGAGGTCAAGTATCTTTATCAGCCAATCAAGATTTCATCAAAGGATGGGGCACTTCAGGTTCTGAATGCTCATTTTTTTTCAACCACAGAGGATTTGGAGTTCAGCTGGAGCATTTATGGTGATGGTCTTGAACTTGGAAATGGAATTCTCTCTCTTCCTGTAATAGGTCCTCAGGAAAGTTATAATATTGAATGGCAAACAAGTCCATGGTATGATCTATGGGCTTCGTCACCTGCATTAGAGTTCTTTTTAAATGTATCAGTGAAACTTCTGCACTCAACACGATGGGTCGAAGCTGGTCACATCATTTCATCATCACAAGTCCAGTTGCCGATGAAACGAGAATTTTTTCCTCATTCCATCAAGAATGGAAGTGCTAGTTTACTTAGTGAAATTCTTGGGGATACAGTTCGAGTCTACCAGCAGAACTTATGGGAGATTAAATTGGATGTTCAAACTGGAACACTCGAAAGCTGGAAGGTTAAAGGAGTTCCTCTAATAATTAAAGGTATTGTACCTTCCTTCTGGCGAGCACCTACTGATAACGACAAGGGTGGAGGTTCATGTAGTTATTTATCCTTGTGGAAAGCTGCCCATATTGACAGCCTCTCTTTCACTGCCGAAAGATGTTCCATACTCAGTACGACAGAACATTATGTGAAAATCGCTGTCGTTTTTCTGGGTCTTGAGAATGATGAGCAGCAGGCTTCTTTCTCTGACTCAGAGAAATCAAATGTCTTAATTCAAGTGGATATGACTTATACAATTTATGGTTCTGGGGATGTGATTGTGGACTGCAATGTACAGCCAAGTCCAAATCTTCCTCCTTTGCCTCGTGTGGGAGTCGAATTTCATCTAGATAAATCTGTGGACCAGGTCAGATGGTATGGAAGAGGGCCGTTTGAGTGCTATCCCGATCGAAAAGCAGCGGCTCATGTTGGAGTTTATGAGAAGAATGTGTCTGAAATGCATGTCCCGTACATTGTCCCTGGAGAATCTTCAGGTAGGGCTGATGTTAGGTGGGTTACTTTTGAAAACAAGGATGGTGTTGGAATCTATGCCTCAATATACAGAGGCTCACCGCCTATGCAAATGAGTGCAAGCTATTACTCCACGGAAGAGCTCGACCGGGCAGTACATAATGAGGACCTTGTTGAGGGAGACGACATTGAGGTTCATCTTGACCACAAGCACATGGGTGTGGGAGGAGACGACAGCTGGTCTCCCTGTGTACATGATGAGTATTTGCTTTCTCCGGTGCCATACTCGTTCTCGATCCGGTTCTATCCGTTAACTCCATCTGCTTCTGGCTATGATGCTTACAAATCCCAACTCCCATTGTAG

Coding sequence (CDS)

AATTCAATTTCTGTTCTGGGTCTGCTCGGATTTCATGGTTTAATTTTGAAACCAGTGCTGTGGCTGCAGGTCATGGCTTCATTGGCTGCTAAGCTTATGATTCCCTCTGAGAATGGTTACAGGGTGTGGGAAGACCAAAATTTCATCAAGTGGAAGAAGAGGGATTCTCACGTTCCTCTACGCTGCCAGGATTCTGTTGAAGGATGCCTCAAGTACTGGCATGACCGTAGTAAAGTAGATTTTCTGGTGTCGAACTCTGCTGTTTGGAATGATGATGCTGTTCAAAGTGCTCTTGACTGTGCTGCTTTCTGGGTTAAGGGCTTACCTTTCATCAAGTCTTTATCTGGTTATTGGAAATTTTATTTGGCCGCTACTCCAACGAGCGTTCCAGAGAATTTTCATGGAAGTGCATTTGAGGATTCTGAATGGACAAATTTGCCAGTTCCTTCAAATTGGCAGATGCATGGGTTTGATCGGCCCATTTATACCAATGTTGTTTATCCCTTCCCTCTCGATCCTCCGCATGTTCCTGAGGACAATCCTACTGGCTGCTACAGAACTTATTTTCAACTTCCAGAAGAATGGAAAGGTCGTAGAATTTTGTTGCACTTTGAAGCTGTTGATTGTGCATTTTTTGTGTGGATAAATGGGAGTCTTGTTGGGTACAGTCAGGACAGTAGACTACCAGCAGAATTTGAAATAACAGAATATTGCCATCCATGTGGCTCAAAGTCCAAGAATGTTCTTGCTGTTCAAGTGTTAAAATGGAGTGATGGTTCTTACCTTGAGGACCAAGACCAGTGGTGGCTCTCAGGAATTCATCGTGATGTCCTTCTCTTATCCAAACCTCAGGTATTCATAGGGGACTACTTTTTCAAATCATGTGTTGGGGAGGATTTTTCTTATGCTGATATACAGGTTGAAGTGAAAATTGACAACTCTCTTGATACAAGCAAGGAAAATTTTCTTAACGACTTTAAGTTAGAAGCAATATTGTTTGACAATAGAACCTGGGACAATCATGAAGGCCAAGCCGATCTGCTCTCATCAAATGTGGCCAATGTTAAGCTTACTCTCCTCTCTGCGACAACTCTGGGGTTCCATGGTTATGTACTTGGGGGAAGATTGGAAAAGCCTAAGCTTTGGTCTGCAGAGCACCCACAGCTCTACACTCTTATTATTCTTCTGAAAGACTTGTCGGATCAGATTGTTGACTGTGAGTCATGCCTAGTTGGAATAAGAAGTATTACAAAAGCTCCAAAACAGCTACTTGTCAATGGATGTCCAGTAGTAATCAGAGGCGTGAATAGGCACGAGCATCATCCGCGTCTTGGGAAGACGAATATAGAGTCCTGCATGGTTCAGGATTTGGTCCTAATGAAACAAAACAACATGAATGCGGTGAGAAACAGCCATTATCCTCAACACCCTCGTTGGTATGAACTGTGTGATTTGTTTGGCATGTACATGATAGACGAGGCCAACATTGAAACACATGGTTTTGATTATACTGGACATGTGAAGCATCCAACTTTGCAACCGAGTTGGGCTGCTGCAATGTTAGATCGTGTAATAGGAATGGTGGAAAGAGACAAGAATCATGCCTGTATTATAGCTTGGTCCTTGGGAAATGAGGCTGGATATGGGCCTAACCACTCAGCTCTAGCTGGGTGGATTCGTGGAAAAGATTCGTCCCGAGTGTTGCACTATGAAGGAGGAGGGTCTAGAACCTCTTCTACTGATATAATCTGTCCCATGTACATGCGTGTATGGGACATGGTGAAGATTGCTAATGATCCCAATGAAACTCGTCCTTTGATTCTCTGCGAGTACTCGCACTCAATGGGAAACAGCACTGGGAATCTTCATAAATATTGGGAAGCAATTGACAACACCTTTGGCCTCCAAGGAGGGTTTATTTGGGATTGGGTTGACCAGGCACTTCTGAAGGAGGTTGACAATGGCAGGAAGCGATGGGCATATGGAGGTGAATTTGGGGATGTCCCAAATGATTCAAATTTCTGTCTCAACGGTGTGACATGGCCAGATCGAACTCCGCATCCTGCACTGCATGAGGTCAAGTATCTTTATCAGCCAATCAAGATTTCATCAAAGGATGGGGCACTTCAGGTTCTGAATGCTCATTTTTTTTCAACCACAGAGGATTTGGAGTTCAGCTGGAGCATTTATGGTGATGGTCTTGAACTTGGAAATGGAATTCTCTCTCTTCCTGTAATAGGTCCTCAGGAAAGTTATAATATTGAATGGCAAACAAGTCCATGGTATGATCTATGGGCTTCGTCACCTGCATTAGAGTTCTTTTTAAATGTATCAGTGAAACTTCTGCACTCAACACGATGGGTCGAAGCTGGTCACATCATTTCATCATCACAAGTCCAGTTGCCGATGAAACGAGAATTTTTTCCTCATTCCATCAAGAATGGAAGTGCTAGTTTACTTAGTGAAATTCTTGGGGATACAGTTCGAGTCTACCAGCAGAACTTATGGGAGATTAAATTGGATGTTCAAACTGGAACACTCGAAAGCTGGAAGGTTAAAGGAGTTCCTCTAATAATTAAAGGTATTGTACCTTCCTTCTGGCGAGCACCTACTGATAACGACAAGGGTGGAGGTTCATGTAGTTATTTATCCTTGTGGAAAGCTGCCCATATTGACAGCCTCTCTTTCACTGCCGAAAGATGTTCCATACTCAGTACGACAGAACATTATGTGAAAATCGCTGTCGTTTTTCTGGGTCTTGAGAATGATGAGCAGCAGGCTTCTTTCTCTGACTCAGAGAAATCAAATGTCTTAATTCAAGTGGATATGACTTATACAATTTATGGTTCTGGGGATGTGATTGTGGACTGCAATGTACAGCCAAGTCCAAATCTTCCTCCTTTGCCTCGTGTGGGAGTCGAATTTCATCTAGATAAATCTGTGGACCAGGTCAGATGGTATGGAAGAGGGCCGTTTGAGTGCTATCCCGATCGAAAAGCAGCGGCTCATGTTGGAGTTTATGAGAAGAATGTGTCTGAAATGCATGTCCCGTACATTGTCCCTGGAGAATCTTCAGGTAGGGCTGATGTTAGGTGGGTTACTTTTGAAAACAAGGATGGTGTTGGAATCTATGCCTCAATATACAGAGGCTCACCGCCTATGCAAATGAGTGCAAGCTATTACTCCACGGAAGAGCTCGACCGGGCAGTACATAATGAGGACCTTGTTGAGGGAGACGACATTGAGGTTCATCTTGACCACAAGCACATGGGTGTGGGAGGAGACGACAGCTGGTCTCCCTGTGTACATGATGAGTATTTGCTTTCTCCGGTGCCATACTCGTTCTCGATCCGGTTCTATCCGTTAACTCCATCTGCTTCTGGCTATGATGCTTACAAATCCCAACTCCCATTGTAG

Protein sequence

NSISVLGLLGFHGLILKPVLWLQVMASLAAKLMIPSENGYRVWEDQNFIKWKKRDSHVPLRCQDSVEGCLKYWHDRSKVDFLVSNSAVWNDDAVQSALDCAAFWVKGLPFIKSLSGYWKFYLAATPTSVPENFHGSAFEDSEWTNLPVPSNWQMHGFDRPIYTNVVYPFPLDPPHVPEDNPTGCYRTYFQLPEEWKGRRILLHFEAVDCAFFVWINGSLVGYSQDSRLPAEFEITEYCHPCGSKSKNVLAVQVLKWSDGSYLEDQDQWWLSGIHRDVLLLSKPQVFIGDYFFKSCVGEDFSYADIQVEVKIDNSLDTSKENFLNDFKLEAILFDNRTWDNHEGQADLLSSNVANVKLTLLSATTLGFHGYVLGGRLEKPKLWSAEHPQLYTLIILLKDLSDQIVDCESCLVGIRSITKAPKQLLVNGCPVVIRGVNRHEHHPRLGKTNIESCMVQDLVLMKQNNMNAVRNSHYPQHPRWYELCDLFGMYMIDEANIETHGFDYTGHVKHPTLQPSWAAAMLDRVIGMVERDKNHACIIAWSLGNEAGYGPNHSALAGWIRGKDSSRVLHYEGGGSRTSSTDIICPMYMRVWDMVKIANDPNETRPLILCEYSHSMGNSTGNLHKYWEAIDNTFGLQGGFIWDWVDQALLKEVDNGRKRWAYGGEFGDVPNDSNFCLNGVTWPDRTPHPALHEVKYLYQPIKISSKDGALQVLNAHFFSTTEDLEFSWSIYGDGLELGNGILSLPVIGPQESYNIEWQTSPWYDLWASSPALEFFLNVSVKLLHSTRWVEAGHIISSSQVQLPMKREFFPHSIKNGSASLLSEILGDTVRVYQQNLWEIKLDVQTGTLESWKVKGVPLIIKGIVPSFWRAPTDNDKGGGSCSYLSLWKAAHIDSLSFTAERCSILSTTEHYVKIAVVFLGLENDEQQASFSDSEKSNVLIQVDMTYTIYGSGDVIVDCNVQPSPNLPPLPRVGVEFHLDKSVDQVRWYGRGPFECYPDRKAAAHVGVYEKNVSEMHVPYIVPGESSGRADVRWVTFENKDGVGIYASIYRGSPPMQMSASYYSTEELDRAVHNEDLVEGDDIEVHLDHKHMGVGGDDSWSPCVHDEYLLSPVPYSFSIRFYPLTPSASGYDAYKSQLPL
Homology
BLAST of Spg009799 vs. NCBI nr
Match: XP_038891496.1 (beta-galactosidase isoform X1 [Benincasa hispida])

HSP 1 Score: 2217.6 bits (5745), Expect = 0.0e+00
Identity = 1025/1114 (92.01%), Postives = 1073/1114 (96.32%), Query Frame = 0

Query: 25   MASLAAKLMIPSENGYRVWEDQNFIKWKKRDSHVPLRCQDSVEGCLKYWHDRSKVDFLVS 84
            MA+LA+KL+IPSENGYR WEDQ FIKW+KRDSHVPLRCQDSVEGCLKYW DRSKVDFLVS
Sbjct: 1    MAALASKLLIPSENGYRAWEDQTFIKWRKRDSHVPLRCQDSVEGCLKYWQDRSKVDFLVS 60

Query: 85   NSAVWNDDAVQSALDCAAFWVKGLPFIKSLSGYWKFYLAATPTSVPENFHGSAFEDSEWT 144
            NSAVWNDDAVQSALD AAFWVK LPFIKSLSGYWKFYLAA+PTSVPENFHGS FEDSEWT
Sbjct: 61   NSAVWNDDAVQSALDSAAFWVKDLPFIKSLSGYWKFYLAASPTSVPENFHGSVFEDSEWT 120

Query: 145  NLPVPSNWQMHGFDRPIYTNVVYPFPLDPPHVPEDNPTGCYRTYFQLPEEWKGRRILLHF 204
             LPVPSNWQMHGFDRPIYTNVVYPFPLDPPHVPEDNPTGCYRTYF LPEEWKGRRILLHF
Sbjct: 121  TLPVPSNWQMHGFDRPIYTNVVYPFPLDPPHVPEDNPTGCYRTYFHLPEEWKGRRILLHF 180

Query: 205  EAVDCAFFVWINGSLVGYSQDSRLPAEFEITEYCHPCGSKSKNVLAVQVLKWSDGSYLED 264
            EAVD AFFVWINGSLVGYSQDSRLPAEFE+TEYCHPCGS+SKNVLAVQVLKWSDGSYLED
Sbjct: 181  EAVDSAFFVWINGSLVGYSQDSRLPAEFEVTEYCHPCGSESKNVLAVQVLKWSDGSYLED 240

Query: 265  QDQWWLSGIHRDVLLLSKPQVFIGDYFFKSCVGEDFSYADIQVEVKIDNSLDTSKENFLN 324
            QDQWWLSGIHRDVLLLSKPQVFIGDYFFKS VGEDFSYADIQVEVKIDNSL+T KENFLN
Sbjct: 241  QDQWWLSGIHRDVLLLSKPQVFIGDYFFKSHVGEDFSYADIQVEVKIDNSLETRKENFLN 300

Query: 325  DFKLEAILFDNRTWDNHEGQADLLSSNVANVKLTLLSATTLGFHGYVLGGRLEKPKLWSA 384
            DFKLEA+LFDN TW+NHEG  DLLSSN+ANVKL+LLS TTLGFHGY+LGGRLEKPKLWSA
Sbjct: 301  DFKLEAVLFDNGTWENHEGNVDLLSSNMANVKLSLLSVTTLGFHGYILGGRLEKPKLWSA 360

Query: 385  EHPQLYTLIILLKDLSDQIVDCESCLVGIRSITKAPKQLLVNGCPVVIRGVNRHEHHPRL 444
            E P LYTLI+LLKD SDQIVDCESCLVGIRSITKAPKQLLVNGCPVVIRGVNRHEHHPRL
Sbjct: 361  EQPHLYTLIVLLKDSSDQIVDCESCLVGIRSITKAPKQLLVNGCPVVIRGVNRHEHHPRL 420

Query: 445  GKTNIESCMVQDLVLMKQNNMNAVRNSHYPQHPRWYELCDLFGMYMIDEANIETHGFDYT 504
            GKTNIESCMVQDLVLMKQ+N+NAVRNSHYPQH RWYELCDLFGMYM+DEANIETHGFD++
Sbjct: 421  GKTNIESCMVQDLVLMKQHNINAVRNSHYPQHSRWYELCDLFGMYMVDEANIETHGFDFS 480

Query: 505  GHVKHPTLQPSWAAAMLDRVIGMVERDKNHACIIAWSLGNEAGYGPNHSALAGWIRGKDS 564
            GHVKHPTLQPSWA+AMLDRVIGMVERDKNHACII WSLGNE+GYGPNHSALAGWIRGKDS
Sbjct: 481  GHVKHPTLQPSWASAMLDRVIGMVERDKNHACIIVWSLGNESGYGPNHSALAGWIRGKDS 540

Query: 565  SRVLHYEGGGSRTSSTDIICPMYMRVWDMVKIANDPNETRPLILCEYSHSMGNSTGNLHK 624
            SRVLHYEGGGSRTSSTDIICPMYMRVWD+VKIANDPNETRPLILCEYSHSMGNSTGNLHK
Sbjct: 541  SRVLHYEGGGSRTSSTDIICPMYMRVWDIVKIANDPNETRPLILCEYSHSMGNSTGNLHK 600

Query: 625  YWEAIDNTFGLQGGFIWDWVDQALLKEVDNGRKRWAYGGEFGDVPNDSNFCLNGVTWPDR 684
            YWEAIDNTFGLQGGFIWDWVDQALLKEV NGRKRWAYGGEFGD+PNDS FCLNGVTWPDR
Sbjct: 601  YWEAIDNTFGLQGGFIWDWVDQALLKEVGNGRKRWAYGGEFGDIPNDSTFCLNGVTWPDR 660

Query: 685  TPHPALHEVKYLYQPIKISSKDGALQVLNAHFFSTTEDLEFSWSIYGDGLELGNGILSLP 744
            TPHPALHEVKYL+Q IKISSKDG L+V+N HFFSTTEDLEFSWSIYGDGLELGNGILSLP
Sbjct: 661  TPHPALHEVKYLHQAIKISSKDGILKVMNDHFFSTTEDLEFSWSIYGDGLELGNGILSLP 720

Query: 745  VIGPQESYNIEWQTSPWYDLWASSPALEFFLNVSVKLLHSTRWVEAGHIISSSQVQLPMK 804
            VIGPQ SY+IEWQ+SPWY LWASSPALEFF+ VSVKLL S RW +AGH +S SQVQLPMK
Sbjct: 721  VIGPQGSYHIEWQSSPWYKLWASSPALEFFITVSVKLLRSMRWAKAGHTVSLSQVQLPMK 780

Query: 805  REFFPHSIKNGSASLLSEILGDTVRVYQQNLWEIKLDVQTGTLESWKVKGVPLIIKGIVP 864
            REFFPHSIKNGS++L++EILGDTVRVYQQNLWEIKLDVQTGTLESWKVKGVPLIIKGI+P
Sbjct: 781  REFFPHSIKNGSSTLVNEILGDTVRVYQQNLWEIKLDVQTGTLESWKVKGVPLIIKGIIP 840

Query: 865  SFWRAPTDNDKGGGSCSYLSLWKAAHIDSLSFTAERCSILSTTEHYVKIAVVFLGLENDE 924
            SFWRAPTDNDKGGGSCSYLSLWKAAHIDSLSFTAERCSILSTTEHYVKIAVVFLG+ +D+
Sbjct: 841  SFWRAPTDNDKGGGSCSYLSLWKAAHIDSLSFTAERCSILSTTEHYVKIAVVFLGVGSDD 900

Query: 925  QQASFSDSEKSNVLIQVDMTYTIYGSGDVIVDCNVQPSPNLPPLPRVGVEFHLDKSVDQV 984
            QQAS SDSEKSN LIQVDMTYTI+GSGDVIVDCNVQPSPNLPPLPRVGV+FHLDKS+D+V
Sbjct: 901  QQASSSDSEKSNALIQVDMTYTIFGSGDVIVDCNVQPSPNLPPLPRVGVKFHLDKSMDRV 960

Query: 985  RWYGRGPFECYPDRKAAAHVGVYEKNVSEMHVPYIVPGESSGRADVRWVTFENKDGVGIY 1044
            RWYGRGPFECYPDRKAAAHVGVYEKNVSEMHVPYIVPGESSGRADVRWVTFENKDGVG+Y
Sbjct: 961  RWYGRGPFECYPDRKAAAHVGVYEKNVSEMHVPYIVPGESSGRADVRWVTFENKDGVGMY 1020

Query: 1045 ASIYRGSPPMQMSASYYSTEELDRAVHNEDLVEGDDIEVHLDHKHMGVGGDDSWSPCVHD 1104
            ASIY  SPPMQM ASYYST EL+RAVHNEDLVEGDDIEVHLDHKHMGVGGDDSWSPCVH+
Sbjct: 1021 ASIYGSSPPMQMRASYYSTAELERAVHNEDLVEGDDIEVHLDHKHMGVGGDDSWSPCVHE 1080

Query: 1105 EYLLSPVPYSFSIRFYPLTPSASGYDAYKSQLPL 1139
            EYLLSPVPYSFSIRFYP+TPS SGYDAY+SQLPL
Sbjct: 1081 EYLLSPVPYSFSIRFYPVTPSTSGYDAYRSQLPL 1114

BLAST of Spg009799 vs. NCBI nr
Match: XP_023526876.1 (uncharacterized protein LOC111790243 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 2189.1 bits (5671), Expect = 0.0e+00
Identity = 1013/1114 (90.93%), Postives = 1069/1114 (95.96%), Query Frame = 0

Query: 25   MASLAAKLMIPSENGYRVWEDQNFIKWKKRDSHVPLRCQDSVEGCLKYWHDRSKVDFLVS 84
            MA L AKL+IPS+NGY+VWED NFIKWKKRDSHVPLRCQDSVEGCLKYWHDRSKVDFLVS
Sbjct: 1    MAVLGAKLLIPSDNGYKVWEDPNFIKWKKRDSHVPLRCQDSVEGCLKYWHDRSKVDFLVS 60

Query: 85   NSAVWNDDAVQSALDCAAFWVKGLPFIKSLSGYWKFYLAATPTSVPENFHGSAFEDSEWT 144
            NSAVWNDDAVQSALD AAFWVKGLPFIKSLSGYWKFYLAA+PTSVPE+FHGSAFEDSEWT
Sbjct: 61   NSAVWNDDAVQSALDSAAFWVKGLPFIKSLSGYWKFYLAASPTSVPEDFHGSAFEDSEWT 120

Query: 145  NLPVPSNWQMHGFDRPIYTNVVYPFPLDPPHVPEDNPTGCYRTYFQLPEEWKGRRILLHF 204
             LPVPSNWQMHGFDRPIYTNVVYPFPLDPPHVPEDNPTGCYRTYF LPEEWKGRRILLHF
Sbjct: 121  TLPVPSNWQMHGFDRPIYTNVVYPFPLDPPHVPEDNPTGCYRTYFHLPEEWKGRRILLHF 180

Query: 205  EAVDCAFFVWINGSLVGYSQDSRLPAEFEITEYCHPCGSKSKNVLAVQVLKWSDGSYLED 264
            EAVD AFFVWINGSLVGYSQDSRLPAEFEITEYCHPCGSKSKNVLAVQVL+WSDGSYLED
Sbjct: 181  EAVDSAFFVWINGSLVGYSQDSRLPAEFEITEYCHPCGSKSKNVLAVQVLRWSDGSYLED 240

Query: 265  QDQWWLSGIHRDVLLLSKPQVFIGDYFFKSCVGEDFSYADIQVEVKIDNSLDTSKENFLN 324
            QDQWWLSGIHRDVLLLSKPQVFIGDYFFKS VGEDFS+ADIQVEVKIDNSL+T KENFLN
Sbjct: 241  QDQWWLSGIHRDVLLLSKPQVFIGDYFFKSHVGEDFSWADIQVEVKIDNSLETKKENFLN 300

Query: 325  DFKLEAILFDNRTWDNHEGQADLLSSNVANVKLTLLSATTLGFHGYVLGGRLEKPKLWSA 384
            D+KLEA+LFDN T DNHEG ADLLSSNVA+VKL+LLSATTLGFHGYVLGGR+EKPKLWSA
Sbjct: 301  DYKLEAVLFDNGTLDNHEGIADLLSSNVASVKLSLLSATTLGFHGYVLGGRMEKPKLWSA 360

Query: 385  EHPQLYTLIILLKDLSDQIVDCESCLVGIRSITKAPKQLLVNGCPVVIRGVNRHEHHPRL 444
            E P LYTLI+LLKD SD+I+DCESCLVGIRSITKAPKQLLVNGCPVVIRGVNRHEHHPRL
Sbjct: 361  EQPHLYTLIVLLKDSSDKIIDCESCLVGIRSITKAPKQLLVNGCPVVIRGVNRHEHHPRL 420

Query: 445  GKTNIESCMVQDLVLMKQNNMNAVRNSHYPQHPRWYELCDLFGMYMIDEANIETHGFDYT 504
            GKTNIESCMV+DLVLMKQNN+NAVRNSHYPQHPRWYELCDLFGMYM+DEANIETHGFD +
Sbjct: 421  GKTNIESCMVKDLVLMKQNNINAVRNSHYPQHPRWYELCDLFGMYMVDEANIETHGFDLS 480

Query: 505  GHVKHPTLQPSWAAAMLDRVIGMVERDKNHACIIAWSLGNEAGYGPNHSALAGWIRGKDS 564
            GHVKHPTLQPSWAAAMLDRVIGMVERDKNHACII WSLGNE+GYGPNHSALAGWIRGKD 
Sbjct: 481  GHVKHPTLQPSWAAAMLDRVIGMVERDKNHACIIVWSLGNESGYGPNHSALAGWIRGKDP 540

Query: 565  SRVLHYEGGGSRTSSTDIICPMYMRVWDMVKIANDPNETRPLILCEYSHSMGNSTGNLHK 624
            SRVLHYEGGGSRTSSTDIICPMYMRVWD+VKIANDP+ETRPLILCEYSHSMGNSTGNLHK
Sbjct: 541  SRVLHYEGGGSRTSSTDIICPMYMRVWDIVKIANDPDETRPLILCEYSHSMGNSTGNLHK 600

Query: 625  YWEAIDNTFGLQGGFIWDWVDQALLKEVDNGRKRWAYGGEFGDVPNDSNFCLNGVTWPDR 684
            YWEAIDNTFGLQGGFIWDWVDQALLKEV NGRKRWAYGGEFGD+PNDS FC+NGVTWPDR
Sbjct: 601  YWEAIDNTFGLQGGFIWDWVDQALLKEVGNGRKRWAYGGEFGDIPNDSVFCMNGVTWPDR 660

Query: 685  TPHPALHEVKYLYQPIKISSKDGALQVLNAHFFSTTEDLEFSWSIYGDGLELGNGILSLP 744
            TPHPAL+EVK LYQ IK S KDG LQVLNAHFF+TTE LEFSWSIYGDGLELGNGILSLP
Sbjct: 661  TPHPALNEVKNLYQEIKFSFKDGTLQVLNAHFFTTTEGLEFSWSIYGDGLELGNGILSLP 720

Query: 745  VIGPQESYNIEWQTSPWYDLWASSPALEFFLNVSVKLLHSTRWVEAGHIISSSQVQLPMK 804
            V+GPQ ++NI+WQ+SPWYDLWASSPALEFFL VSVK LHSTRWV+AGH ISSSQVQLPMK
Sbjct: 721  VLGPQGTHNIDWQSSPWYDLWASSPALEFFLTVSVKRLHSTRWVDAGHTISSSQVQLPMK 780

Query: 805  REFFPHSIKNGSASLLSEILGDTVRVYQQNLWEIKLDVQTGTLESWKVKGVPLIIKGIVP 864
            REFFPHSIKN +++LLSEILGDTVRVYQQ+LWEIKLDVQTGTLESWKVKGVPLIIKGI+P
Sbjct: 781  REFFPHSIKNENSTLLSEILGDTVRVYQQDLWEIKLDVQTGTLESWKVKGVPLIIKGIMP 840

Query: 865  SFWRAPTDNDKGGGSCSYLSLWKAAHIDSLSFTAERCSILSTTEHYVKIAVVFLGLENDE 924
            SFWRAPTDNDKGGGSCSY SLWKAAHIDSLSFTAERCSILSTTEHYVK+A+VFLG+ +D+
Sbjct: 841  SFWRAPTDNDKGGGSCSYFSLWKAAHIDSLSFTAERCSILSTTEHYVKVAIVFLGVGSDD 900

Query: 925  QQASFSDSEKSNVLIQVDMTYTIYGSGDVIVDCNVQPSPNLPPLPRVGVEFHLDKSVDQV 984
            QQAS SDSEKSN+LIQVDMTYTI+GSGDVIVDC VQPSPNLPPLPRVGV+F+LDKS+D+V
Sbjct: 901  QQASSSDSEKSNILIQVDMTYTIFGSGDVIVDCKVQPSPNLPPLPRVGVKFNLDKSMDRV 960

Query: 985  RWYGRGPFECYPDRKAAAHVGVYEKNVSEMHVPYIVPGESSGRADVRWVTFENKDGVGIY 1044
            RWYGRGPFECYPDRKAAAHVGVYEKNVSEMHVPYIVPGESSGR DVRWVT ENKDG+GIY
Sbjct: 961  RWYGRGPFECYPDRKAAAHVGVYEKNVSEMHVPYIVPGESSGRTDVRWVTLENKDGIGIY 1020

Query: 1045 ASIYRGSPPMQMSASYYSTEELDRAVHNEDLVEGDDIEVHLDHKHMGVGGDDSWSPCVHD 1104
            ASIY  SPPMQMSASYYS+ ELDRA HNE+LVEGDDIEVHLDHKHMGVGGDDSWSPCVH+
Sbjct: 1021 ASIYGSSPPMQMSASYYSSAELDRAEHNEELVEGDDIEVHLDHKHMGVGGDDSWSPCVHE 1080

Query: 1105 EYLLSPVPYSFSIRFYPLTPSASGYDAYKSQLPL 1139
            EYLL PVPYSFSIRF P+TPS S YDAY+SQLP+
Sbjct: 1081 EYLLPPVPYSFSIRFCPVTPSTSAYDAYRSQLPV 1114

BLAST of Spg009799 vs. NCBI nr
Match: KAG6601128.1 (hypothetical protein SDJN03_06361, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 2188.7 bits (5670), Expect = 0.0e+00
Identity = 1015/1117 (90.87%), Postives = 1071/1117 (95.88%), Query Frame = 0

Query: 22   LQVMASLAAKLMIPSENGYRVWEDQNFIKWKKRDSHVPLRCQDSVEGCLKYWHDRSKVDF 81
            LQVMA L AKL+IPS+NGY+VWED NFIKWKKRDSHVPLRCQDSVEGCLKYWHDRSKVDF
Sbjct: 30   LQVMAVLGAKLVIPSDNGYKVWEDPNFIKWKKRDSHVPLRCQDSVEGCLKYWHDRSKVDF 89

Query: 82   LVSNSAVWNDDAVQSALDCAAFWVKGLPFIKSLSGYWKFYLAATPTSVPENFHGSAFEDS 141
            LVSNSAVWNDDAVQSALD AAFWVKGLPFIKSLSGYWKFYLAA+PTSVPE+FHGSAFEDS
Sbjct: 90   LVSNSAVWNDDAVQSALDSAAFWVKGLPFIKSLSGYWKFYLAASPTSVPEDFHGSAFEDS 149

Query: 142  EWTNLPVPSNWQMHGFDRPIYTNVVYPFPLDPPHVPEDNPTGCYRTYFQLPEEWKGRRIL 201
            EWT LPVPSNWQMHGFDRPIYTNVVYPFPLDPPHVPEDNPTGCYRTYF LPEEWKGRRIL
Sbjct: 150  EWTTLPVPSNWQMHGFDRPIYTNVVYPFPLDPPHVPEDNPTGCYRTYFHLPEEWKGRRIL 209

Query: 202  LHFEAVDCAFFVWINGSLVGYSQDSRLPAEFEITEYCHPCGSKSKNVLAVQVLKWSDGSY 261
            LHFEAVD AFFVWINGSLVGYSQDSRLPAEFEITEYCHPCGSKSKNVLAVQVL+WSDGSY
Sbjct: 210  LHFEAVDSAFFVWINGSLVGYSQDSRLPAEFEITEYCHPCGSKSKNVLAVQVLRWSDGSY 269

Query: 262  LEDQDQWWLSGIHRDVLLLSKPQVFIGDYFFKSCVGEDFSYADIQVEVKIDNSLDTSKEN 321
            LEDQDQWWLSGIHRDVLLLSKPQVFIGDYFFKS VGEDFS+ADIQVEVKIDNSL+T KEN
Sbjct: 270  LEDQDQWWLSGIHRDVLLLSKPQVFIGDYFFKSHVGEDFSWADIQVEVKIDNSLETKKEN 329

Query: 322  FLNDFKLEAILFDNRTWDNHEGQADLLSSNVANVKLTLLSATTLGFHGYVLGGRLEKPKL 381
            FLNDFKLEA+LFDN T DNHEG ADLLSSNVA+VKL+LLSATTLGFHGYVLGGR+EKPKL
Sbjct: 330  FLNDFKLEAVLFDNGTLDNHEGIADLLSSNVASVKLSLLSATTLGFHGYVLGGRMEKPKL 389

Query: 382  WSAEHPQLYTLIILLKDLSDQIVDCESCLVGIRSITKAPKQLLVNGCPVVIRGVNRHEHH 441
            WSAE P LYTLI+LLKD SD+I+DCESCLVGIRSITKAPKQLLVNG PVVIRGVNRHEHH
Sbjct: 390  WSAEQPHLYTLIVLLKDSSDKIIDCESCLVGIRSITKAPKQLLVNGYPVVIRGVNRHEHH 449

Query: 442  PRLGKTNIESCMVQDLVLMKQNNMNAVRNSHYPQHPRWYELCDLFGMYMIDEANIETHGF 501
            PRLGKTNIESCMV+DLVLMKQNN+NAVRNSHYPQHPRWYELCDLFGMY++DEANIETHGF
Sbjct: 450  PRLGKTNIESCMVKDLVLMKQNNINAVRNSHYPQHPRWYELCDLFGMYVVDEANIETHGF 509

Query: 502  DYTGHVKHPTLQPSWAAAMLDRVIGMVERDKNHACIIAWSLGNEAGYGPNHSALAGWIRG 561
            D +GHVKHPTLQPSWAAAMLDRVIGMVERDKNHACII WSLGNE+GYGPNHSALAGWIRG
Sbjct: 510  DLSGHVKHPTLQPSWAAAMLDRVIGMVERDKNHACIIVWSLGNESGYGPNHSALAGWIRG 569

Query: 562  KDSSRVLHYEGGGSRTSSTDIICPMYMRVWDMVKIANDPNETRPLILCEYSHSMGNSTGN 621
            KD SRVLHYEGGGSRTSSTDIICPMYMRVWD+VKIANDP+ETRPLILCEYSHSMGNSTGN
Sbjct: 570  KDPSRVLHYEGGGSRTSSTDIICPMYMRVWDIVKIANDPDETRPLILCEYSHSMGNSTGN 629

Query: 622  LHKYWEAIDNTFGLQGGFIWDWVDQALLKEVDNGRKRWAYGGEFGDVPNDSNFCLNGVTW 681
            LHKYWEAIDNTFGLQGGFIWDWVDQALLKEV NGRKRWAYGGEFGD+PNDS FC+NGVTW
Sbjct: 630  LHKYWEAIDNTFGLQGGFIWDWVDQALLKEVGNGRKRWAYGGEFGDIPNDSVFCMNGVTW 689

Query: 682  PDRTPHPALHEVKYLYQPIKISSKDGALQVLNAHFFSTTEDLEFSWSIYGDGLELGNGIL 741
            PDRTPHPAL+EVK LYQ IK S KDG L+VLNAHFF+TTEDLEFSWSIYGDGLELGNGIL
Sbjct: 690  PDRTPHPALNEVKNLYQEIKFSFKDGTLKVLNAHFFTTTEDLEFSWSIYGDGLELGNGIL 749

Query: 742  SLPVIGPQESYNIEWQTSPWYDLWASSPALEFFLNVSVKLLHSTRWVEAGHIISSSQVQL 801
            SLPV+GPQ ++NIEWQ+SPWYDLWASSPALEFFL VSVK LHSTRWV+AGH ISSSQVQL
Sbjct: 750  SLPVLGPQGTHNIEWQSSPWYDLWASSPALEFFLTVSVKRLHSTRWVDAGHTISSSQVQL 809

Query: 802  PMKREFFPHSIKNGSASLLSEILGDTVRVYQQNLWEIKLDVQTGTLESWKVKGVPLIIKG 861
            PMKREFFPHSIKN +++LLSEILGDTVRVYQQ+LWEIKLDVQTGTLESWKVKGVPLIIKG
Sbjct: 810  PMKREFFPHSIKNENSTLLSEILGDTVRVYQQDLWEIKLDVQTGTLESWKVKGVPLIIKG 869

Query: 862  IVPSFWRAPTDNDKGGGSCSYLSLWKAAHIDSLSFTAERCSILSTTEHYVKIAVVFLGLE 921
            I+PSFWRAPTDNDKGGGSCSY SLWKAAHID LSFTAERCSILSTTEHYVK+A+VFLG+ 
Sbjct: 870  IMPSFWRAPTDNDKGGGSCSYFSLWKAAHIDILSFTAERCSILSTTEHYVKVAIVFLGVG 929

Query: 922  NDEQQASFSDSEKSNVLIQVDMTYTIYGSGDVIVDCNVQPSPNLPPLPRVGVEFHLDKSV 981
            +D+QQAS SDSEKSN+LIQVDMTYTI+GSGDVIVDC VQPSPNLPPLPRVGV+F+LDKS+
Sbjct: 930  SDDQQASSSDSEKSNILIQVDMTYTIFGSGDVIVDCKVQPSPNLPPLPRVGVKFNLDKSM 989

Query: 982  DQVRWYGRGPFECYPDRKAAAHVGVYEKNVSEMHVPYIVPGESSGRADVRWVTFENKDGV 1041
            D+VRWYGRGPFECYPDRKAAAHVGVYEKNVSEMHVPYIVPGESSGR DVRWVT ENKDG+
Sbjct: 990  DRVRWYGRGPFECYPDRKAAAHVGVYEKNVSEMHVPYIVPGESSGRTDVRWVTLENKDGI 1049

Query: 1042 GIYASIYRGSPPMQMSASYYSTEELDRAVHNEDLVEGDDIEVHLDHKHMGVGGDDSWSPC 1101
            GIYASIY  SPPMQMSASYYS+ ELDRA HNE+LVEGDDIEVHLDHKHMGVGGDDSWSPC
Sbjct: 1050 GIYASIYGSSPPMQMSASYYSSAELDRAEHNEELVEGDDIEVHLDHKHMGVGGDDSWSPC 1109

Query: 1102 VHDEYLLSPVPYSFSIRFYPLTPSASGYDAYKSQLPL 1139
            VHDEYLL PVPYSFSIRF P+TPS S Y+AY+SQLP+
Sbjct: 1110 VHDEYLLPPVPYSFSIRFCPVTPSTSAYEAYRSQLPV 1146

BLAST of Spg009799 vs. NCBI nr
Match: KAG7031926.1 (lacZ [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 2187.9 bits (5668), Expect = 0.0e+00
Identity = 1013/1114 (90.93%), Postives = 1067/1114 (95.78%), Query Frame = 0

Query: 25   MASLAAKLMIPSENGYRVWEDQNFIKWKKRDSHVPLRCQDSVEGCLKYWHDRSKVDFLVS 84
            MA L AKL+IPS+NGY+VWED NFIKWKKRDSHVPLRCQDSVEGCLKYWHDRSKVDFLVS
Sbjct: 1    MAVLGAKLVIPSDNGYKVWEDPNFIKWKKRDSHVPLRCQDSVEGCLKYWHDRSKVDFLVS 60

Query: 85   NSAVWNDDAVQSALDCAAFWVKGLPFIKSLSGYWKFYLAATPTSVPENFHGSAFEDSEWT 144
            NSAVWNDDAVQSALD AAFWVKGLPFIKSLSGYWKFYLAA+PTSVPE+FHGSAFEDSEWT
Sbjct: 61   NSAVWNDDAVQSALDSAAFWVKGLPFIKSLSGYWKFYLAASPTSVPEDFHGSAFEDSEWT 120

Query: 145  NLPVPSNWQMHGFDRPIYTNVVYPFPLDPPHVPEDNPTGCYRTYFQLPEEWKGRRILLHF 204
             LPVPSNWQMHGFDRPIYTNVVYPFPLDPPHVPEDNPTGCYRTYF LPEEWKGRRILLHF
Sbjct: 121  TLPVPSNWQMHGFDRPIYTNVVYPFPLDPPHVPEDNPTGCYRTYFHLPEEWKGRRILLHF 180

Query: 205  EAVDCAFFVWINGSLVGYSQDSRLPAEFEITEYCHPCGSKSKNVLAVQVLKWSDGSYLED 264
            EAVD AFFVWINGSLVGYSQDSRLPAEFEITEYCHPCGS SKNVLAVQVL+WSDGSYLED
Sbjct: 181  EAVDSAFFVWINGSLVGYSQDSRLPAEFEITEYCHPCGSNSKNVLAVQVLRWSDGSYLED 240

Query: 265  QDQWWLSGIHRDVLLLSKPQVFIGDYFFKSCVGEDFSYADIQVEVKIDNSLDTSKENFLN 324
            QDQWWLSGIHRDVLLLSKPQVFIGDYFFKS VGEDFS+ADIQVEVKIDNSL+T KENFLN
Sbjct: 241  QDQWWLSGIHRDVLLLSKPQVFIGDYFFKSHVGEDFSWADIQVEVKIDNSLETKKENFLN 300

Query: 325  DFKLEAILFDNRTWDNHEGQADLLSSNVANVKLTLLSATTLGFHGYVLGGRLEKPKLWSA 384
            DFKLEA+LFDN T DNHEG ADLLSSNVA+VKL+LLSATTLGFHGYVLGGR+EKPKLWSA
Sbjct: 301  DFKLEAVLFDNGTLDNHEGIADLLSSNVASVKLSLLSATTLGFHGYVLGGRMEKPKLWSA 360

Query: 385  EHPQLYTLIILLKDLSDQIVDCESCLVGIRSITKAPKQLLVNGCPVVIRGVNRHEHHPRL 444
            E P LYTLI+LLKD SD+I+DCESCLVGIRSITKAPKQLLVNGCPVVIRGVNRHEHHPRL
Sbjct: 361  EQPHLYTLIVLLKDSSDKIIDCESCLVGIRSITKAPKQLLVNGCPVVIRGVNRHEHHPRL 420

Query: 445  GKTNIESCMVQDLVLMKQNNMNAVRNSHYPQHPRWYELCDLFGMYMIDEANIETHGFDYT 504
            GKTNIESCMV+DLVLMKQNN+NAVRNSHYPQHPRWYELCDLFGMYM+DEANIETHGFD +
Sbjct: 421  GKTNIESCMVKDLVLMKQNNINAVRNSHYPQHPRWYELCDLFGMYMVDEANIETHGFDLS 480

Query: 505  GHVKHPTLQPSWAAAMLDRVIGMVERDKNHACIIAWSLGNEAGYGPNHSALAGWIRGKDS 564
            GHVKHPTLQPSWAAAMLDRVIGMVERDKNHACII WSLGNE+GYGPNHSALAGWIRGKD 
Sbjct: 481  GHVKHPTLQPSWAAAMLDRVIGMVERDKNHACIIVWSLGNESGYGPNHSALAGWIRGKDP 540

Query: 565  SRVLHYEGGGSRTSSTDIICPMYMRVWDMVKIANDPNETRPLILCEYSHSMGNSTGNLHK 624
            SRVLHYEGGGSRTSSTDIICPMYMRVWD+VKIANDP+ETRPLILCEYSHSMGNSTGNLHK
Sbjct: 541  SRVLHYEGGGSRTSSTDIICPMYMRVWDIVKIANDPDETRPLILCEYSHSMGNSTGNLHK 600

Query: 625  YWEAIDNTFGLQGGFIWDWVDQALLKEVDNGRKRWAYGGEFGDVPNDSNFCLNGVTWPDR 684
            YWEAIDNTFGLQGGFIWDWVDQALLKEV NGRKRWAYGGEFGD+PNDS FC+NGVTWPDR
Sbjct: 601  YWEAIDNTFGLQGGFIWDWVDQALLKEVGNGRKRWAYGGEFGDIPNDSVFCMNGVTWPDR 660

Query: 685  TPHPALHEVKYLYQPIKISSKDGALQVLNAHFFSTTEDLEFSWSIYGDGLELGNGILSLP 744
            TPHPAL+EVK LYQ IK S KDG L+VLNAHFF+TTEDLEFSWSIYGDGLELGNGILSLP
Sbjct: 661  TPHPALNEVKNLYQEIKFSFKDGTLKVLNAHFFTTTEDLEFSWSIYGDGLELGNGILSLP 720

Query: 745  VIGPQESYNIEWQTSPWYDLWASSPALEFFLNVSVKLLHSTRWVEAGHIISSSQVQLPMK 804
            V+GPQ ++NIEWQ+SPWYDLWASSPALEFFL VSVK LHSTRWV AGH ISSSQVQLPMK
Sbjct: 721  VLGPQGTHNIEWQSSPWYDLWASSPALEFFLTVSVKRLHSTRWVNAGHTISSSQVQLPMK 780

Query: 805  REFFPHSIKNGSASLLSEILGDTVRVYQQNLWEIKLDVQTGTLESWKVKGVPLIIKGIVP 864
            REFFPHSIKN +++LLSEILGDTVRVYQQ+LWEIKLDVQTGTLESWKVKGVPLIIKGI+P
Sbjct: 781  REFFPHSIKNENSTLLSEILGDTVRVYQQDLWEIKLDVQTGTLESWKVKGVPLIIKGIMP 840

Query: 865  SFWRAPTDNDKGGGSCSYLSLWKAAHIDSLSFTAERCSILSTTEHYVKIAVVFLGLENDE 924
            SFWRAPTDNDKGGGSCSY SLWKAAHIDSLSFTAERCSILSTTEHYVK+A+ FLG+ +D+
Sbjct: 841  SFWRAPTDNDKGGGSCSYFSLWKAAHIDSLSFTAERCSILSTTEHYVKVAIAFLGVGSDD 900

Query: 925  QQASFSDSEKSNVLIQVDMTYTIYGSGDVIVDCNVQPSPNLPPLPRVGVEFHLDKSVDQV 984
            QQAS SDSEKSN+LIQVDMTYTI+GSGDVIVDC VQPSPNLPPLPRVGV+F+LDKS+D+V
Sbjct: 901  QQASSSDSEKSNILIQVDMTYTIFGSGDVIVDCKVQPSPNLPPLPRVGVKFNLDKSMDRV 960

Query: 985  RWYGRGPFECYPDRKAAAHVGVYEKNVSEMHVPYIVPGESSGRADVRWVTFENKDGVGIY 1044
            RWYGRGPFECYPDRKAAAHVGVYEKNVSEMHVPYIVPGESSGR DVRWVT ENKDG+GIY
Sbjct: 961  RWYGRGPFECYPDRKAAAHVGVYEKNVSEMHVPYIVPGESSGRTDVRWVTLENKDGIGIY 1020

Query: 1045 ASIYRGSPPMQMSASYYSTEELDRAVHNEDLVEGDDIEVHLDHKHMGVGGDDSWSPCVHD 1104
            ASIY  SPPMQMSASYYS+ ELDRA HNE+LVEGDDIEVHLDHKHMGVGGDDSWSPCVHD
Sbjct: 1021 ASIYGSSPPMQMSASYYSSAELDRAEHNEELVEGDDIEVHLDHKHMGVGGDDSWSPCVHD 1080

Query: 1105 EYLLSPVPYSFSIRFYPLTPSASGYDAYKSQLPL 1139
            EYLL PVPYSFSIRF P+TPS S Y+AY+SQLP+
Sbjct: 1081 EYLLPPVPYSFSIRFCPVTPSTSAYEAYRSQLPV 1114

BLAST of Spg009799 vs. NCBI nr
Match: XP_022956821.1 (uncharacterized protein LOC111458406 [Cucurbita moschata])

HSP 1 Score: 2186.0 bits (5663), Expect = 0.0e+00
Identity = 1011/1114 (90.75%), Postives = 1069/1114 (95.96%), Query Frame = 0

Query: 25   MASLAAKLMIPSENGYRVWEDQNFIKWKKRDSHVPLRCQDSVEGCLKYWHDRSKVDFLVS 84
            MA L AKL+IPS+NGY+VWED NFIKWKKRDSHVPLRCQDSVEGCLKYWHDRSKVDFLVS
Sbjct: 1    MAVLGAKLVIPSDNGYKVWEDPNFIKWKKRDSHVPLRCQDSVEGCLKYWHDRSKVDFLVS 60

Query: 85   NSAVWNDDAVQSALDCAAFWVKGLPFIKSLSGYWKFYLAATPTSVPENFHGSAFEDSEWT 144
            NSAVWNDDAVQSALD AAFWVKGLPFIKSLSGYWKFYLAA+PTSVP++FHGSAFEDSEWT
Sbjct: 61   NSAVWNDDAVQSALDSAAFWVKGLPFIKSLSGYWKFYLAASPTSVPKDFHGSAFEDSEWT 120

Query: 145  NLPVPSNWQMHGFDRPIYTNVVYPFPLDPPHVPEDNPTGCYRTYFQLPEEWKGRRILLHF 204
             LPVPSNWQMHGFDRPIYTN+VYPFPLDPPHVPEDNPTGCYRTYF LPEEWKGRRILLHF
Sbjct: 121  TLPVPSNWQMHGFDRPIYTNIVYPFPLDPPHVPEDNPTGCYRTYFHLPEEWKGRRILLHF 180

Query: 205  EAVDCAFFVWINGSLVGYSQDSRLPAEFEITEYCHPCGSKSKNVLAVQVLKWSDGSYLED 264
            EAVD AFFVWINGSLVGYSQDSRLPAEFEIT+YCHPCGSKSKNVLAVQVL+WSDGSYLED
Sbjct: 181  EAVDSAFFVWINGSLVGYSQDSRLPAEFEITKYCHPCGSKSKNVLAVQVLRWSDGSYLED 240

Query: 265  QDQWWLSGIHRDVLLLSKPQVFIGDYFFKSCVGEDFSYADIQVEVKIDNSLDTSKENFLN 324
            QDQWWLSGIHRDVLLLSKPQVFIGDYFFKS VGEDFS+ADIQVEVKIDNSL+T KENFLN
Sbjct: 241  QDQWWLSGIHRDVLLLSKPQVFIGDYFFKSHVGEDFSWADIQVEVKIDNSLETKKENFLN 300

Query: 325  DFKLEAILFDNRTWDNHEGQADLLSSNVANVKLTLLSATTLGFHGYVLGGRLEKPKLWSA 384
            DFKLEA+LFDN T DNHEG ADLLSS+VA+VKL+LLSATTLGFHGYVLGGR+EKPKLWSA
Sbjct: 301  DFKLEAVLFDNGTLDNHEGIADLLSSSVASVKLSLLSATTLGFHGYVLGGRMEKPKLWSA 360

Query: 385  EHPQLYTLIILLKDLSDQIVDCESCLVGIRSITKAPKQLLVNGCPVVIRGVNRHEHHPRL 444
            E P LYTLI+LLKD SD+I+DCESCLVGIRSITKAPKQLLVNGCPVVIRGVNRHEHHPRL
Sbjct: 361  EQPHLYTLIVLLKDSSDKIIDCESCLVGIRSITKAPKQLLVNGCPVVIRGVNRHEHHPRL 420

Query: 445  GKTNIESCMVQDLVLMKQNNMNAVRNSHYPQHPRWYELCDLFGMYMIDEANIETHGFDYT 504
            GKTNIESCMV+DLVLMKQNN+NAVRNSHYPQHPRWYELCDLFGMYM+DEANIETHGFD +
Sbjct: 421  GKTNIESCMVKDLVLMKQNNINAVRNSHYPQHPRWYELCDLFGMYMVDEANIETHGFDLS 480

Query: 505  GHVKHPTLQPSWAAAMLDRVIGMVERDKNHACIIAWSLGNEAGYGPNHSALAGWIRGKDS 564
            GHVKHPTLQPSWAAAMLDRVIGMVERDKNHACII WSLGNE+GYGPNHSALAGWIRGKD 
Sbjct: 481  GHVKHPTLQPSWAAAMLDRVIGMVERDKNHACIIVWSLGNESGYGPNHSALAGWIRGKDP 540

Query: 565  SRVLHYEGGGSRTSSTDIICPMYMRVWDMVKIANDPNETRPLILCEYSHSMGNSTGNLHK 624
            SRVLHYEGGGSRTSSTDIICPMYMRVWD+VKIANDP+ETRPLILCEYSHSMGNSTGNLHK
Sbjct: 541  SRVLHYEGGGSRTSSTDIICPMYMRVWDIVKIANDPDETRPLILCEYSHSMGNSTGNLHK 600

Query: 625  YWEAIDNTFGLQGGFIWDWVDQALLKEVDNGRKRWAYGGEFGDVPNDSNFCLNGVTWPDR 684
            YWEAIDNTFGLQGGFIWDWVDQALLKEV NGRKRWAYGGEFGD+PNDS FC+NGVTWPDR
Sbjct: 601  YWEAIDNTFGLQGGFIWDWVDQALLKEVGNGRKRWAYGGEFGDIPNDSVFCMNGVTWPDR 660

Query: 685  TPHPALHEVKYLYQPIKISSKDGALQVLNAHFFSTTEDLEFSWSIYGDGLELGNGILSLP 744
            TPHPAL+EVK LYQ IK S KDG L+VLNAHFF+TTEDLEFSWSIYGDGLELGNGILSLP
Sbjct: 661  TPHPALNEVKNLYQEIKFSFKDGTLKVLNAHFFTTTEDLEFSWSIYGDGLELGNGILSLP 720

Query: 745  VIGPQESYNIEWQTSPWYDLWASSPALEFFLNVSVKLLHSTRWVEAGHIISSSQVQLPMK 804
            V+GPQ ++NIEWQ+SPWYDLWASSPALEFFL VSVK LHSTRWV AGH ISSSQVQLPMK
Sbjct: 721  VLGPQGTHNIEWQSSPWYDLWASSPALEFFLTVSVKRLHSTRWVNAGHTISSSQVQLPMK 780

Query: 805  REFFPHSIKNGSASLLSEILGDTVRVYQQNLWEIKLDVQTGTLESWKVKGVPLIIKGIVP 864
            REFFPHSIKN +++LLSEILGDTVRVYQQ+LWEIKLDVQTGTLESWKVKGVPLIIKGI+P
Sbjct: 781  REFFPHSIKNENSTLLSEILGDTVRVYQQDLWEIKLDVQTGTLESWKVKGVPLIIKGIMP 840

Query: 865  SFWRAPTDNDKGGGSCSYLSLWKAAHIDSLSFTAERCSILSTTEHYVKIAVVFLGLENDE 924
            SFWRAPTDNDKGGGSCSY SLWKAAHIDSLSFTAERCSILSTTEHYVK+A+VFLG+ +D+
Sbjct: 841  SFWRAPTDNDKGGGSCSYFSLWKAAHIDSLSFTAERCSILSTTEHYVKVAIVFLGVGSDD 900

Query: 925  QQASFSDSEKSNVLIQVDMTYTIYGSGDVIVDCNVQPSPNLPPLPRVGVEFHLDKSVDQV 984
            QQAS SDSEKSN+LIQVDMTYTI+GSGDVIVDC VQPSPNLPPLPRVGV+F+LDKS+D+V
Sbjct: 901  QQASSSDSEKSNILIQVDMTYTIFGSGDVIVDCKVQPSPNLPPLPRVGVKFNLDKSMDRV 960

Query: 985  RWYGRGPFECYPDRKAAAHVGVYEKNVSEMHVPYIVPGESSGRADVRWVTFENKDGVGIY 1044
            RWYGRGPFECYPDRKAAAHVGVYEKNVSEMHVPYIVPGESSGR DVRWVT ENKDG+GIY
Sbjct: 961  RWYGRGPFECYPDRKAAAHVGVYEKNVSEMHVPYIVPGESSGRTDVRWVTLENKDGIGIY 1020

Query: 1045 ASIYRGSPPMQMSASYYSTEELDRAVHNEDLVEGDDIEVHLDHKHMGVGGDDSWSPCVHD 1104
            ASIY  SPPMQMSASYYS+ ELDRA HNE+LVEGDDIEVHLDHKHMGVGGDDSWSPCVHD
Sbjct: 1021 ASIYGSSPPMQMSASYYSSAELDRAEHNEELVEGDDIEVHLDHKHMGVGGDDSWSPCVHD 1080

Query: 1105 EYLLSPVPYSFSIRFYPLTPSASGYDAYKSQLPL 1139
            EYLL PVPYSFSIRF P+TPS S Y+AY+SQLP+
Sbjct: 1081 EYLLPPVPYSFSIRFCPVTPSTSAYEAYRSQLPV 1114

BLAST of Spg009799 vs. ExPASy Swiss-Prot
Match: A5F5U6 (Beta-galactosidase OS=Vibrio cholerae serotype O1 (strain ATCC 39541 / Classical Ogawa 395 / O395) OX=345073 GN=lacZ PE=3 SV=2)

HSP 1 Score: 779.2 bits (2011), Expect = 6.2e-224
Identity = 435/1092 (39.84%), Postives = 586/1092 (53.66%), Query Frame = 0

Query: 43   WEDQNFIKWKKRDSHVPLRCQDSVEGCLKYWHDRSKVDFLVSNSAVWNDDAVQSALDCAA 102
            W++ + +KW  R  HVPL    + +                           ++ LD   
Sbjct: 13   WQNPHIVKWHCRTPHVPLHSYRTEQ---------------------------EARLDVGG 72

Query: 103  FWVKGLPFIKSLSGYWKFYLAATPTSVPENFHGSAFEDSEWTNLPVPSNWQMHGFDRPIY 162
                     +SL+G W+F L   P +V        F+DS W ++PVPSNWQM GFD+PIY
Sbjct: 73   -------NRQSLNGQWRFALFEKPEAVEPAVIDPDFDDSAWAHIPVPSNWQMQGFDKPIY 132

Query: 163  TNVVYPFPLDPPHVPEDNPTGCYRTYFQLPEEWKGRRILLHFEAVDCAFFVWINGSLVGY 222
            TN+ YPF   PP+VP+DNPTGCYR  F L ++     I + F+ V+ AF +W NG  VGY
Sbjct: 133  TNIQYPFADRPPYVPQDNPTGCYRHRFTLEKQALTESIRIVFDGVNSAFHLWCNGHWVGY 192

Query: 223  SQDSRLPAEFEITEYCHPCGSKSKNVLAVQVLKWSDGSYLEDQDQWWLSGIHRDVLLLSK 282
            SQDSRLPAEFE+T Y      + +N+L   VL+WSDGSYLEDQD WWLSGI RDV L  K
Sbjct: 193  SQDSRLPAEFELTPYL----QEGENLLVAMVLRWSDGSYLEDQDMWWLSGIFRDVYLYRK 252

Query: 283  PQVFIGDYFFKSCVGEDFSYADIQVEVKIDNSLDTSKENFLNDFKLEAILFDNRTWDNHE 342
            P + I D+F ++ +   + +A+++VE ++               +++  LFD +      
Sbjct: 253  PILAIEDFFIRTELDALYQHAELRVETRL--------SQVTRHHQVQVALFDAQGECVAR 312

Query: 343  GQADLLSSNVANVKLTLLSATTLGFHGYVLGGRLEKPKLWSAEHPQLYTLIILLKDLSDQ 402
             QA      V + K      T    H   +      P LWS E P LY  +I L D    
Sbjct: 313  SQALHTGQRVVDEKGAWHDKTE---HSLAIC----SPTLWSDEAPYLYRCVICLLDEDGA 372

Query: 403  IVDCESCLVGIRSITKAPKQLLVNGCPVVIRGVNRHEHHPRLGKTNIESCMVQDLVLMKQ 462
             ++ ES  VG R +      L +NG P++IRGVNRHEHHP LG    E+ M +D+ LMKQ
Sbjct: 373  PIEFESAAVGFRKVEITQGLLKLNGQPLLIRGVNRHEHHPELGHVMDEASMRRDIELMKQ 432

Query: 463  NNMNAVRNSHYPQHPRWYELCDLFGMYMIDEANIETHGFDYTGHVKHPTLQPSWAAAMLD 522
            +N NAVR +HYP HPRWYELCD +G+Y++DEAN+ETHG      + +    P W  A L 
Sbjct: 433  HNFNAVRTAHYPNHPRWYELCDEYGLYVVDEANLETHGQFPMSRLSN---DPQWVNAYLQ 492

Query: 523  RVIGMVERDKNHACIIAWSLGNEAGYGPNHSALAGWIRGKDSSRVLHYEGGGSRTSSTDI 582
            R+IGMVERDKNH C+I WSLGNE+G G NH A+  W + +D SR + YEGGG+ T++TDI
Sbjct: 493  RMIGMVERDKNHPCVIIWSLGNESGIGTNHHAMYQWTKQRDPSRPVQYEGGGANTAATDI 552

Query: 583  ICPMYMRV-----------WDMVKIANDPNETRPLILCEYSHSMGNSTGNLHKYWEAIDN 642
            +CPMY RV           + +    + P E RPLILCEY+H+MGNS G  +KYW+A   
Sbjct: 553  VCPMYARVDQHQPHPAVPKYALKNWISLPQENRPLILCEYAHAMGNSLGAFYKYWQAFRE 612

Query: 643  TFGLQGGFIWDWVDQALLKEVDNGRKRWAYGGEFGDVPNDSNFCLNGVTWPDRTPHPALH 702
               LQGGFIWDWVDQ + K    GR  W YGG+FGD  ND  FC+NG+ +PDRTPHPALH
Sbjct: 613  FPRLQGGFIWDWVDQGISKWDSEGRHYWGYGGDFGDTINDRQFCINGLLFPDRTPHPALH 672

Query: 703  EVKYLYQPIKISSKDGALQVLNAHFFSTTEDLEFSWSIYGDGLELGNGILSLPVIGPQES 762
            EVK + QP + S     L + N   F+    LE   S+  DG E+    L L  I P+ +
Sbjct: 673  EVKKVQQPYQFSLSYPKLTIHNERLFAAL-PLELVVSVLCDGQEIKQERLPLD-IAPRGT 732

Query: 763  YNIEWQTSPWYDLWASSPALEFFLNVSVKLLHSTRWVEAGHIISSSQVQLPMKREFFPHS 822
              ++  + P        P  E+ LN  +       W  AGH I+S Q  L  +R   P  
Sbjct: 733  ITLDLASLPML------PEHEYHLNAVLLCREDQPWSNAGHCIASEQWCLQPRRSMLPKI 792

Query: 823  IKNGSASLLSEILGDTVRVYQQNLWEIKLDVQTGTLESWKVKGVPLIIKGIVPSFWRAPT 882
                      +  GD VR+   N  + + + QTG LE W   G P++ + +  +F+RA  
Sbjct: 793  THAPLPQWQQD--GDKVRIEAAN-QQWQFNRQTGLLEQWWQNGQPVLSEPLRDNFYRAVL 852

Query: 883  DNDKGGGSC------SYLSLWKAAHIDSLSFTAERCSILSTTEHYVKIAVVFLGLENDEQ 942
            DND G          S+++ W AA +D L      C  L  T     + VV + + +  Q
Sbjct: 853  DNDIGTSEAQHLDPNSWIARWHAAGLDKLRV---ECDDLRVTTLNESVEVV-IDVAHYHQ 912

Query: 943  QASFSDSEKSNVLIQVDMTYTIYGSGDVIVDCNVQPSPNLPPLPRVGVEFHLDKSVDQVR 1002
            QA         + ++    Y I+G   V ++  V    +LPPLPRVG+   L  + + V 
Sbjct: 913  QA---------LALRTRWRYQIFGDARVELNVEVMLCSDLPPLPRVGLTLALPVAENPVS 972

Query: 1003 WYGRGPFECYPDRKAAAHVGVYEKNVSEMHVPYIVPGESSGRADVRWVTFENKDGVGIYA 1062
            W+GRGP E YPDR  +AHVG Y   V E+H PYI P E+  R D R +         + A
Sbjct: 973  WFGRGPHENYPDRLQSAHVGRYTATVDELHTPYIFPSENGLRCDTRQLQ--------VGA 1014

Query: 1063 SIYRGSPPMQMSASYYSTEELDRAVHNEDLVEGDDIEVHLDHKHMGVGGDDSWSPCVHDE 1118
             +  G      S S YS   LD+A H+ +LV GD   ++LD +HMGVGGDDSWS  VH E
Sbjct: 1033 LVVEGH--FHFSLSRYSQTMLDKAKHSNELVAGDKWYLNLDAQHMGVGGDDSWSQSVHPE 1014

BLAST of Spg009799 vs. ExPASy Swiss-Prot
Match: Q6LL68 (Beta-galactosidase OS=Photobacterium profundum (strain SS9) OX=298386 GN=lacZ PE=3 SV=1)

HSP 1 Score: 766.9 bits (1979), Expect = 3.2e-220
Identity = 418/1035 (40.39%), Postives = 575/1035 (55.56%), Query Frame = 0

Query: 112  KSLSGYWKFYLAATPTSVPENFHGSAFEDSEWTNLPVPSNWQMHGFDRPIYTNVVYPFPL 171
            +SL+G WKF L   P  V   F    F DS W ++ VPSNWQ+ G+D+PIY NV YPF +
Sbjct: 47   QSLNGQWKFKLFDAPEQVEGEFIDVQFNDSAWGDITVPSNWQLQGYDKPIYANVKYPFEV 106

Query: 172  DPPHVPEDNPTGCYRTYFQLPEEWKGRRILLHFEAVDCAFFVWINGSLVGYSQDSRLPAE 231
            +PP+VP DNPTGCYRT   L E        + F+ V+ AF +W NG  VGYSQDSRLPAE
Sbjct: 107  NPPYVPADNPTGCYRTRLTLTEADLESTQRIIFDGVNSAFHLWCNGDWVGYSQDSRLPAE 166

Query: 232  FEITEYCHPCGSKSKNVLAVQVLKWSDGSYLEDQDQWWLSGIHRDVLLLSKPQVFIGDYF 291
            F++++Y     +  +N LAV V++WSDGSYLEDQD WWLSGI RDV LLSKP+  I D F
Sbjct: 167  FDLSQYL----TAGENTLAVMVIRWSDGSYLEDQDMWWLSGIFRDVTLLSKPKQCIEDVF 226

Query: 292  FKSCVGEDFSYADIQVEVKIDNSLDTSKENFLNDFKLEAILFDNRTWDNHEGQADLLSSN 351
                +  D  Y D  + +    S   + +  +  F     + +      H  + D   S 
Sbjct: 227  ITPDL--DACYRDGSLSIVTHISAPETSQVHVQLFDGSQAVTEPSIARPHNRRIDERGSY 286

Query: 352  VANVKLTLLSATTLGFHGYVLGGRLEKPKLWSAEHPQLYTLIILLKDLSDQIVDCESCLV 411
               V  TL                + +P+ W+AE P LY +++ L D     ++ E+  V
Sbjct: 287  DDVVFQTL---------------HVREPQQWTAETPNLYRVVVSLLDAEGNHLESEAYQV 346

Query: 412  GIRSITKAPKQLLVNGCPVVIRGVNRHEHHPRLGKTNIESCMVQDLVLMKQNNMNAVRNS 471
            G R +     QL +NG P++IRGVNRHEHHP LG    E  MV+D+ LMKQ N NAVR +
Sbjct: 347  GFRKVEVKDGQLQLNGKPLLIRGVNRHEHHPELGHVMTEEDMVRDICLMKQYNFNAVRTA 406

Query: 472  HYPQHPRWYELCDLFGMYMIDEANIETHGFDYTGHVKHPTLQPSWAAAMLDRVIGMVERD 531
            HYP HPRWYELCD +G+Y+ DEANIETHG      +   +  P WA A + R   MV RD
Sbjct: 407  HYPNHPRWYELCDQYGLYVCDEANIETHGMIPMNRL---SADPQWAHAYMSRYTQMVMRD 466

Query: 532  KNHACIIAWSLGNEAGYGPNHSALAGWIRGKDSSRVLHYEGGGSRTSSTDIICPMYMRV- 591
            KNH  II WSLGNE+G+G +H+A+  W +  D SR + YEGGG+ T++TDIICPMY RV 
Sbjct: 467  KNHPSIIIWSLGNESGHGSSHNAMYAWSKQFDPSRPVQYEGGGANTTATDIICPMYARVN 526

Query: 592  ----------WDMVKIANDPNETRPLILCEYSHSMGNSTGNLHKYWEAIDNTFGLQGGFI 651
                      W + +  + PNE RPLILCEY+H+MGNS GN ++YW+A      LQGGFI
Sbjct: 527  TTVEDEAVPKWPIKQWISLPNEQRPLILCEYAHAMGNSLGNFNEYWDAFREFPRLQGGFI 586

Query: 652  WDWVDQALLKEVDNGRKRWAYGGEFGDVPNDSNFCLNGVTWPDRTPHPALHEVKYLYQPI 711
            WDWVDQ L +  ++G+  WAYGG+FGD  ND  FC+NG+ +PDRTPHP L EVK+  + I
Sbjct: 587  WDWVDQGLSQWDNDGKHFWAYGGDFGDTINDRQFCINGLIFPDRTPHPTLEEVKFCQRMI 646

Query: 712  KIS-----SKDGALQVLNAHFFSTTEDLEFSWSIYGDGLELGNGILSLPV-IGPQESYNI 771
             ++      +   L V N + F +T++ +  WS+  +G+E+ +G  +L +  G Q++ +I
Sbjct: 647  TVALTQQDKQQCHLTVTNEYVFRSTDNEQLHWSVLENGVEVQSGQCTLAIDAGSQQTVDI 706

Query: 772  EWQTSPWYDLWASSPALEFFLNVSVKLLHSTRWVEAGHIISSSQVQLPMKREFFPHSIKN 831
                 P  D        ++ LN  + L+ +T W +AGH+ ++ Q  L          I  
Sbjct: 707  ALDFQPKAD-------AKYHLNTDICLISATPWAQAGHVSATEQFTLSNTSSLTLPKISI 766

Query: 832  GSASLLSEILGDTVRVYQQNLWEIKLDVQTGTLESWKVKGVPLIIKGIVPSFWRAPTDND 891
             SA  LSE   D +        + + +V++G L SW V G   ++     +F+RAP DND
Sbjct: 767  LSAPQLSEQGRDILVSNLDKKHQWQWNVESGLLTSWMVDGQSQLLHAPEDNFFRAPLDND 826

Query: 892  KGGGSC------SYLSLWKAAHIDSLSFTAERCSILSTTEHYVKIAVVFLGLENDEQQAS 951
             G          +++  W AA I         C+  S ++  VK+   F    N   QA 
Sbjct: 827  IGVSEIDNIDPNAWVCRWDAAGIGRWERECVSCTSESLSQ-AVKVTSTFAYHHNGGVQA- 886

Query: 952  FSDSEKSNVLIQVDMTYTIYGSGDVIVDCNVQPSPNLPPLPRVGVEFHLDKSVDQ--VRW 1011
                      I V  TYT+   G++ +D +V  + +LPP+PR+G+E  L    D   V W
Sbjct: 887  ----------ITV-WTYTLDNQGEMHIDVDVTLADHLPPMPRIGLELALPLPSDNTTVTW 946

Query: 1012 YGRGPFECYPDRKAAAHVGVYEKNVSEMHVPYIVPGESSGRADVRWVTFENKDGVGIYAS 1071
             G GPFE YPDR AAA  G + +++  MH PYI P +S  R+  +W+   N +  G +  
Sbjct: 947  QGLGPFENYPDRLAAARFGQHTQSLDAMHTPYIFPTDSGLRSGTQWLNVGNLECTGDFL- 1006

Query: 1072 IYRGSPPMQMSASYYSTEELDRAVHNEDLVEGDDIEVHLDHKHMGVGGDDSWSPCVHDEY 1122
                      S S +S ++L  A H  +L   D I + +DH+HMGVGGDDSWSP VH+E+
Sbjct: 1007 ---------FSVSRFSQQQLTEAKHTNELTLEDKIYLRIDHQHMGVGGDDSWSPSVHEEF 1027

BLAST of Spg009799 vs. ExPASy Swiss-Prot
Match: Q8D4H3 (Beta-galactosidase OS=Vibrio vulnificus (strain CMCP6) OX=216895 GN=lacZ PE=3 SV=2)

HSP 1 Score: 763.1 bits (1969), Expect = 4.6e-219
Identity = 416/1047 (39.73%), Postives = 582/1047 (55.59%), Query Frame = 0

Query: 112  KSLSGYWKFYLAATPTSVPENFHGSAFEDSEWTNLPVPSNWQMHGFDRPIYTNVVYPFPL 171
            +SL+G WKF L   P  V   F  + F D+EW  +PVPSNWQ+ G+D+PIY N+ YPF +
Sbjct: 48   QSLNGQWKFKLFDAPEQVDGQFTQADFNDAEWDEIPVPSNWQLQGYDKPIYANIKYPFDV 107

Query: 172  DPPHVPEDNPTGCYRTYFQL-PEEWKGRRILLHFEAVDCAFFVWINGSLVGYSQDSRLPA 231
            +PP VP DNPTGCYRT   L PE+    + ++ F+ V+ AF +W NG+ VGYSQDSRLPA
Sbjct: 108  NPPFVPSDNPTGCYRTRVSLSPEDLLNTQRII-FDGVNSAFHLWCNGTWVGYSQDSRLPA 167

Query: 232  EFEITEYCHPCGSKSKNVLAVQVLKWSDGSYLEDQDQWWLSGIHRDVLLLSKPQVFIGDY 291
            EF++T +        +N LAV V++W DGSYLEDQD WWLSGI RDV LLSKPQ  I D 
Sbjct: 168  EFDLTSHL----VAGENTLAVMVMRWCDGSYLEDQDMWWLSGIFRDVTLLSKPQHCIEDV 227

Query: 292  FFKSCVGEDFSYADIQVEVKIDNSLDTSKENFLNDFKLEAILFDNRTWDNHEGQADLLSS 351
            F    +  D  Y D  + +    +   +       ++++  LF        EG   +   
Sbjct: 228  FITPEL--DACYRDGSLSIVTTIAAPET-------YQVQVQLF--------EGTQAVTEP 287

Query: 352  NVANVKLTLLSATTLGFHGYVLGG-RLEKPKLWSAEHPQLYTLIILLKDLSDQIVDCESC 411
            N+A      +          V     L +PK W+AE P LY L++ L D +   ++ E+ 
Sbjct: 288  NIARPHNRRIDERGTWNDDVVFQTLHLREPKKWTAETPNLYRLVVSLLDENGTHLESEAY 347

Query: 412  LVGIRSITKAPKQLLVNGCPVVIRGVNRHEHHPRLGKTNIESCMVQDLVLMKQNNMNAVR 471
             VG R +  +  QL +NG P++IRGVNRHEHHP LG    E  M++D+ LMKQ N NAVR
Sbjct: 348  PVGFRKVEISEGQLKLNGKPLLIRGVNRHEHHPELGHVMTEEDMIRDICLMKQYNFNAVR 407

Query: 472  NSHYPQHPRWYELCDLFGMYMIDEANIETHGFDYTGHVKHPTLQPSWAAAMLDRVIGMVE 531
             +HYP HPRWYELCD +G+Y+ DEANIETHG      +   +  P WA A + R   MV 
Sbjct: 408  TAHYPNHPRWYELCDQYGLYVCDEANIETHGMQPMSRL---SSDPQWAHAYMSRYTQMVL 467

Query: 532  RDKNHACIIAWSLGNEAGYGPNHSALAGWIRGKDSSRVLHYEGGGSRTSSTDIICPMYMR 591
            RDKNH  II WSLGNE+G+G NH+A+  W +  D SR + YEGGGS T++TDII PMY R
Sbjct: 468  RDKNHPSIIIWSLGNESGHGSNHNAMYAWSKNFDPSRPVQYEGGGSNTTATDIIAPMYAR 527

Query: 592  V-----------WDMVKIANDPNETRPLILCEYSHSMGNSTGNLHKYWEAIDNTFGLQGG 651
            V           W + K  + PNETRPLILCEY+H+MGNS G+  +YW A      LQGG
Sbjct: 528  VNTLVADEAVPKWPIKKWISLPNETRPLILCEYAHAMGNSLGSFDEYWAAFREFPRLQGG 587

Query: 652  FIWDWVDQALLKEVDNGRKRWAYGGEFGDVPNDSNFCLNGVTWPDRTPHPALHEVKYLYQ 711
            FIWDWVDQ L +  +NG+  WAYGG+FGD  ND  FC+NG+ +PDRT HP L E KY  +
Sbjct: 588  FIWDWVDQGLSQWDENGQHFWAYGGDFGDEINDRQFCINGLIFPDRTVHPTLQEAKYCQR 647

Query: 712  PIKIS-----SKDGALQVLNAHFFSTTEDLEFSWSIYGDG--LELGNGILSLPVIGPQES 771
             I +S      K   L V N + F TT++ + +WS+  +G  ++ G+ +LS+     Q  
Sbjct: 648  MITVSLQEQTQKACTLLVTNENLFRTTDNEQLNWSLLENGQVIQTGSQVLSVEA-DSQTR 707

Query: 772  YNIEWQTSPWYDLWASSPALEFFLNVSVKLLHSTRWVEAGHIISSSQVQL---------P 831
              I    +P           +++LN  + L+ +T W  AGH++++ Q+ L          
Sbjct: 708  LEIALNFTP-------KAQAQYYLNTDICLIEATSWAPAGHVVATEQMALRNHAGLAMPT 767

Query: 832  MKREFFPHSIKNGSASLLSEILGDTVRVYQQNLWEIKLDVQTGTLESWKVKGVPLIIKGI 891
            ++ +  P   +NG A ++S +        +++ W  + D Q+G L  W V G   ++   
Sbjct: 768  LRTQPAPKLTENGHAIVVSSL-------DEKHQW--RWDSQSGLLMEWNVDGKAQMLAAP 827

Query: 892  VPSFWRAPTDNDKGGGSC------SYLSLWKAAHIDSLSFTAERCSILSTTEHYVKIAVV 951
              +F+RAP DND G          +++  W+ A I        +C    T  H V +   
Sbjct: 828  QDNFFRAPLDNDIGISEVDNVDPNAWVCRWEMAGIGQWERHCVQCE-SETLAHAVVVTTT 887

Query: 952  FLGLENDEQQASFSDSEKSNVLIQVDMTYTIYGSGDVIVDCNVQPSPNLPPLPRVGVEFH 1011
            F      + QA                T+T+   G++++D +V  +  LPP+PR+G+E  
Sbjct: 888  FAYHFGGDVQAI------------TQWTHTLSNDGEMLLDVDVTLADALPPMPRIGLELQ 947

Query: 1012 --LDKSVDQVRWYGRGPFECYPDRKAAAHVGVYEKNVSEMHVPYIVPGESSGRADVRWVT 1071
              L ++   + W G GPFE YPDR AAA  G++ + +++MH PYI P +S  R   +W+ 
Sbjct: 948  LPLHQADTPITWQGLGPFENYPDRLAAARFGLHTQTLAQMHTPYIFPTDSGLRCGTQWLQ 1007

Query: 1072 FENKDGVGIYASIYRGSPPMQMSASYYSTEELDRAVHNEDLVEGDDIEVHLDHKHMGVGG 1122
              N+  +         S   Q S S Y+ ++L  A H  DL+  + I + LDH+HMGVGG
Sbjct: 1008 V-NELAI---------SGDFQFSVSQYAQQQLAEAKHTHDLLAQERIYLRLDHQHMGVGG 1029

BLAST of Spg009799 vs. ExPASy Swiss-Prot
Match: P81650 (Beta-galactosidase OS=Pseudoalteromonas haloplanktis OX=228 GN=lacZ PE=1 SV=2)

HSP 1 Score: 761.5 bits (1965), Expect = 1.3e-218
Identity = 409/1051 (38.92%), Postives = 582/1051 (55.38%), Query Frame = 0

Query: 112  KSLSGYWKFYLAATPTSVPENFHGSAFE---DSEWTNLPVPSNWQMHGFDRPIYTNVVYP 171
            KSL+G W F L   P +V E+            +W ++ VPSNWQ+HGFD+PIY NV YP
Sbjct: 48   KSLNGQWDFKLFDKPEAVDESLLYEKISKELSGDWQSITVPSNWQLHGFDKPIYCNVKYP 107

Query: 172  FPLDPPHVPEDNPTGCYRTYFQLPEEWKGRRILLHFEAVDCAFFVWINGSLVGYSQDSRL 231
            F ++PP VP DNPTGCYRT F +  E   +R  + FE V+ AF +W NG  VGYSQDSRL
Sbjct: 108  FAVNPPFVPSDNPTGCYRTEFTITPEQLTQRNHIIFEGVNSAFHLWCNGQWVGYSQDSRL 167

Query: 232  PAEFEITEYCHPCGSKSKNVLAVQVLKWSDGSYLEDQDQWWLSGIHRDVLLLSKPQVFIG 291
            P+EF+++E          N +AV V++WSDGSYLEDQD WWLSGI RDV LL+KPQ  I 
Sbjct: 168  PSEFDLSELL----VVGTNRIAVMVIRWSDGSYLEDQDMWWLSGIFRDVNLLTKPQSQIR 227

Query: 292  DYFFKSCVGEDFSYADIQVEVKIDNSLDTSKENFLNDFKLEAILFD-------------- 351
            D F    +  D  Y D  + +K       +  N  N++++   +FD              
Sbjct: 228  DVFITPDL--DACYRDATLHIK-------TAINAPNNYQVAVQIFDGKTSLCEPKIQSTN 287

Query: 352  NRTWDNHEGQADLLSSNVANVKLTLLSATTLGFHGYVLGGRLEKPKLWSAEHPQLYTLII 411
            N+  D   G +D++   +A                      +  PK W+AE P LY  ++
Sbjct: 288  NKRVDEKGGWSDVVFQTIA----------------------IRSPKKWTAETPYLYRCVV 347

Query: 412  LLKDLSDQIVDCESCLVGIRSITKAPKQLLVNGCPVVIRGVNRHEHHPRLGKTNIESCMV 471
             L D     VD E+  +G R +     QL VNG P++IRGVNRHEHHP  G     + M+
Sbjct: 348  SLLDEQGNTVDVEAYNIGFRKVEMLNGQLCVNGKPLLIRGVNRHEHHPENGHAVSTADMI 407

Query: 472  QDLVLMKQNNMNAVRNSHYPQHPRWYELCDLFGMYMIDEANIETHGFDYTGHVKHPTLQP 531
            +D+ LMKQNN NAVR +HYP HP +YELCD  G+Y++DEANIETHG    G +      P
Sbjct: 408  EDIKLMKQNNFNAVRTAHYPNHPLFYELCDELGLYVVDEANIETHGMFPMGRL---ASDP 467

Query: 532  SWAAAMLDRVIGMVERDKNHACIIAWSLGNEAGYGPNHSALAGWIRGKDSSRVLHYEGGG 591
             WA A + R   MVERDKNHA II WSLGNE G+G NH A+ GW +  D SR + YEGGG
Sbjct: 468  LWAGAFMSRYTQMVERDKNHASIIIWSLGNECGHGANHDAMYGWSKSFDPSRPVQYEGGG 527

Query: 592  SRTSSTDIICPMYMRV-----------WDMVKIANDPNETRPLILCEYSHSMGNSTGNLH 651
            + T++TDIICPMY RV           + + K  + P ETRPLILCEY+H+MGNS G+  
Sbjct: 528  ANTTATDIICPMYSRVDTDIKDDAVPKYSIKKWLSLPGETRPLILCEYAHAMGNSLGSFD 587

Query: 652  KYWEAIDNTFGLQGGFIWDWVDQALLKEVDNGRKRWAYGGEFGDVPNDSNFCLNGVTWPD 711
             YW+A      LQGGFIWDWVDQ L K  +NG+  WAYGG+FGD  ND  FC+NG+ +PD
Sbjct: 588  DYWQAFREYPRLQGGFIWDWVDQGLSKIDENGKHYWAYGGDFGDELNDRQFCINGLLFPD 647

Query: 712  RTPHPALHEVKYLYQPIKISSKDG---------ALQVLNAHFFSTTEDLEFSWSIYGDGL 771
            RTPHP+L E KY  Q ++ + ++          ++ V + + F  T++ +  W +  +G+
Sbjct: 648  RTPHPSLFEAKYSQQHLQFTLREQNQNQNQNQYSIDVFSDYVFRHTDNEKLVWQLIQNGV 707

Query: 772  ELGNGILSLPVIGPQESYNIEWQTSPWYDLWASSPALEFFLNVSVKLLHSTRWVEAGHII 831
             +  G ++L  I PQ ++ +  +T   ++  A     +++LN+ V L++ + +  A H++
Sbjct: 708  CVEQGEMALN-IAPQSTHTLTIKTKTAFEHGA-----QYYLNLDVALINDSHFANANHVM 767

Query: 832  SSSQVQLPMKREFFPHSIKNGS-ASLLSEILGDTVRVYQQNLWEIKLDVQTGTLESWKVK 891
             S Q +L         S  + +  S++S    D+    + N +++  + Q+G +E W   
Sbjct: 768  DSEQFKLINSNNLNSKSFASATEKSVISVNETDSHLSIENNTFKLVFNQQSGLIEQWLQD 827

Query: 892  GVPLIIKGIVPSFWRAPTDNDKGGGSCSYL------SLWKAAHIDSLSFTAERCSILSTT 951
               +I   +V +F+RAP DND G      L      + W  A I     T   CS ++  
Sbjct: 828  DTQVISSPLVDNFYRAPLDNDIGVSEVDNLDPNAWEARWSRAGIGQWQRT---CSSINAV 887

Query: 952  EHYVKIAVVFLGLENDEQQASFSDSEKSNVLIQVDMTYTIYGSGDVIVDCNVQPSPNLPP 1011
            +  V + +  +          F+      +  Q    YT+  +G + ++ +V  +  LPP
Sbjct: 888  QSSVDVRITCV----------FNYEFNGVLQAQTQWLYTLNNTGTISLNVDVNLNDTLPP 947

Query: 1012 LPRVGVEFHLDKSVD-QVRWYGRGPFECYPDRKAAAHVGVYEKNVSEMHVPYIVPGESSG 1071
            +PR+G+   ++K  D +V W G GPFE YPDRK+AA  G Y  +++E++ PYI P ++  
Sbjct: 948  MPRIGLSTTINKQSDTKVNWLGLGPFENYPDRKSAARFGYYSLSLNELYTPYIFPTDNGL 1007

Query: 1072 RADVRWVTFENKDGVGIYASIYRGSPPMQMSASYYSTEELDRAVHNEDLVEGDDIEVHLD 1118
            R+D + ++  N    G +            +AS YS   L +A H  +L+  D I VH+D
Sbjct: 1008 RSDCQLLSINNLIVTGAFL----------FAASEYSQNMLTQAKHTNELIADDCIHVHID 1031

BLAST of Spg009799 vs. ExPASy Swiss-Prot
Match: Q7MG04 (Beta-galactosidase OS=Vibrio vulnificus (strain YJ016) OX=196600 GN=lacZ PE=3 SV=1)

HSP 1 Score: 760.8 bits (1963), Expect = 2.3e-218
Identity = 415/1045 (39.71%), Postives = 579/1045 (55.41%), Query Frame = 0

Query: 112  KSLSGYWKFYLAATPTSVPENFHGSAFEDSEWTNLPVPSNWQMHGFDRPIYTNVVYPFPL 171
            +SL+G WKF L   P  V   F  + F D+EW  +PVPSNWQ+HG+D+PIY N+ YPF +
Sbjct: 48   QSLNGQWKFKLFDAPEQVDGQFTQADFNDAEWDEIPVPSNWQLHGYDKPIYANIKYPFDV 107

Query: 172  DPPHVPEDNPTGCYRTYFQL-PEEWKGRRILLHFEAVDCAFFVWINGSLVGYSQDSRLPA 231
            +PP VP +NPTGCYRT   L PE+    + ++ F+ V+ AF +W NG+ VGYSQDSRLPA
Sbjct: 108  NPPFVPRENPTGCYRTRVSLSPEDLLNTQRII-FDGVNSAFHLWCNGTWVGYSQDSRLPA 167

Query: 232  EFEITEYCHPCGSKSKNVLAVQVLKWSDGSYLEDQDQWWLSGIHRDVLLLSKPQVFIGDY 291
            EF++T +        +N LAV V++W DGSYLEDQD WWLSGI RDV LLSKPQ  I D 
Sbjct: 168  EFDLTSHL----VAGENTLAVMVMRWCDGSYLEDQDMWWLSGIFRDVTLLSKPQHCIEDV 227

Query: 292  FFKSCVGEDFSYADIQVEVKIDNSLDTSKENFLNDFKLEAILFDNRTWDNHEGQADLLSS 351
            F    +  D  Y D  + +    +   +       ++++  LF+  T    E   D   +
Sbjct: 228  FITPDL--DACYRDGSLSIVTTIAAPET-------YQVQVQLFEG-TQAVTEPNIDRPHN 287

Query: 352  NVANVKLTLLSATTLGFHGYVLGGRLEKPKLWSAEHPQLYTLIILLKDLSDQIVDCESCL 411
               + + T         H       L +PK W+AE P LY L++ L D +   ++ E+  
Sbjct: 288  RRIDERGTWNDVVFQTLH-------LREPKKWTAETPNLYRLVVSLLDENGTHLESEAYP 347

Query: 412  VGIRSITKAPKQLLVNGCPVVIRGVNRHEHHPRLGKTNIESCMVQDLVLMKQNNMNAVRN 471
            VG R +  +  QL +NG P++IRGVNRHEHHP LG    E  M++D+ LMKQ N NAVR 
Sbjct: 348  VGFRKVEISEGQLKLNGKPLLIRGVNRHEHHPELGHVMTEEDMIRDICLMKQYNFNAVRT 407

Query: 472  SHYPQHPRWYELCDLFGMYMIDEANIETHGFDYTGHVKHPTLQPSWAAAMLDRVIGMVER 531
            +HYP HPRWYELCD +G+Y+ DEANIETHG      +   +  P WA A + R   MV R
Sbjct: 408  AHYPNHPRWYELCDQYGLYVCDEANIETHGMQPMSRL---SSDPQWAHAYMSRYTQMVLR 467

Query: 532  DKNHACIIAWSLGNEAGYGPNHSALAGWIRGKDSSRVLHYEGGGSRTSSTDIICPMYMRV 591
            DKNH  II WSLGNE+G+G NH+A+  W +  D SR + YEGGGS T++TDII PMY RV
Sbjct: 468  DKNHPSIIIWSLGNESGHGSNHNAMYAWSKNFDPSRPVQYEGGGSNTTATDIIAPMYARV 527

Query: 592  -----------WDMVKIANDPNETRPLILCEYSHSMGNSTGNLHKYWEAIDNTFGLQGGF 651
                       W + K  + PNETRPLILCEY+H+MGNS G+  +YW A      LQGGF
Sbjct: 528  NTLIADEAVPKWPIKKWISLPNETRPLILCEYAHAMGNSLGSFDEYWAAFREFPRLQGGF 587

Query: 652  IWDWVDQALLKEVDNGRKRWAYGGEFGDVPNDSNFCLNGVTWPDRTPHPALHEVKYLYQP 711
            IWDWVDQ L +  +NG+  WAYGG+FGD  ND  FC+NG+ +PDRT HP L E KY  + 
Sbjct: 588  IWDWVDQGLSQWDENGQHFWAYGGDFGDEINDRQFCINGLIFPDRTVHPTLQEAKYCQRM 647

Query: 712  IKIS-----SKDGALQVLNAHFFSTTEDLEFSWSIYGDGLELGNGILSLPV-IGPQESYN 771
            I +S      K   L V N + F  T++ + +WS+  +G  +  G L+L V    Q    
Sbjct: 648  ITVSLQEQTQKACTLLVTNENLFRATDNEQLNWSLLENGQVIQTGSLALSVEADSQTRLE 707

Query: 772  IEWQTSPWYDLWASSPALEFFLNVSVKLLHSTRWVEAGHIISSSQVQL---------PMK 831
            I    +P           ++ LN  + L+ +T W  AGH++++ Q+ L          ++
Sbjct: 708  IALNFTP-------KAQAQYHLNTDICLIEATSWAPAGHVVATEQMALRNHAGLAIPTLR 767

Query: 832  REFFPHSIKNGSASLLSEILGDTVRVYQQNLWEIKLDVQTGTLESWKVKGVPLIIKGIVP 891
             +  P   ++G A ++S +        +++ W  + D Q+G L  W V G   ++     
Sbjct: 768  TQPAPKLTQDGHAIVVSSL-------DEKHQW--RWDSQSGLLMEWNVDGKAQMLAAPQD 827

Query: 892  SFWRAPTDNDKGGGSC------SYLSLWKAAHIDSLSFTAERCSILSTTEHYVKIAVVFL 951
            +F+RAP DND G          +++  W+ A I         C    T  H V +   F 
Sbjct: 828  NFFRAPLDNDIGISEVDNVDPNAWVCRWEMAGIGQWERHCVHCD-SETLAHTVVVTTTFA 887

Query: 952  GLENDEQQASFSDSEKSNVLIQVDMTYTIYGSGDVIVDCNVQPSPNLPPLPRVGVEFHLD 1011
                 + QA                T+T+   G++++D +V  +  LPP+PR+G+E  L 
Sbjct: 888  YHFGGDVQAI------------TQWTHTLSNDGEMLLDVDVTLADTLPPMPRIGLELQLP 947

Query: 1012 --KSVDQVRWYGRGPFECYPDRKAAAHVGVYEKNVSEMHVPYIVPGESSGRADVRWVTFE 1071
              ++   + W G GPFE YPDR AAA  G++ + +++MH PYI P +S  R   +W+   
Sbjct: 948  LYQADTPITWQGLGPFENYPDRLAAARFGLHTQTLAQMHTPYIFPTDSGLRCGTQWLQV- 1007

Query: 1072 NKDGVGIYASIYRGSPPMQMSASYYSTEELDRAVHNEDLVEGDDIEVHLDHKHMGVGGDD 1122
            N+  +         S   Q S S Y+ ++L  A H  DL+  + I + LDH+HMGVGGDD
Sbjct: 1008 NELAI---------SGDFQFSVSQYAQQQLAEAKHTHDLLAQERIYLRLDHQHMGVGGDD 1028

BLAST of Spg009799 vs. ExPASy TrEMBL
Match: A0A6J1GXK4 (Lactase OS=Cucurbita moschata OX=3662 GN=LOC111458406 PE=3 SV=1)

HSP 1 Score: 2186.0 bits (5663), Expect = 0.0e+00
Identity = 1011/1114 (90.75%), Postives = 1069/1114 (95.96%), Query Frame = 0

Query: 25   MASLAAKLMIPSENGYRVWEDQNFIKWKKRDSHVPLRCQDSVEGCLKYWHDRSKVDFLVS 84
            MA L AKL+IPS+NGY+VWED NFIKWKKRDSHVPLRCQDSVEGCLKYWHDRSKVDFLVS
Sbjct: 1    MAVLGAKLVIPSDNGYKVWEDPNFIKWKKRDSHVPLRCQDSVEGCLKYWHDRSKVDFLVS 60

Query: 85   NSAVWNDDAVQSALDCAAFWVKGLPFIKSLSGYWKFYLAATPTSVPENFHGSAFEDSEWT 144
            NSAVWNDDAVQSALD AAFWVKGLPFIKSLSGYWKFYLAA+PTSVP++FHGSAFEDSEWT
Sbjct: 61   NSAVWNDDAVQSALDSAAFWVKGLPFIKSLSGYWKFYLAASPTSVPKDFHGSAFEDSEWT 120

Query: 145  NLPVPSNWQMHGFDRPIYTNVVYPFPLDPPHVPEDNPTGCYRTYFQLPEEWKGRRILLHF 204
             LPVPSNWQMHGFDRPIYTN+VYPFPLDPPHVPEDNPTGCYRTYF LPEEWKGRRILLHF
Sbjct: 121  TLPVPSNWQMHGFDRPIYTNIVYPFPLDPPHVPEDNPTGCYRTYFHLPEEWKGRRILLHF 180

Query: 205  EAVDCAFFVWINGSLVGYSQDSRLPAEFEITEYCHPCGSKSKNVLAVQVLKWSDGSYLED 264
            EAVD AFFVWINGSLVGYSQDSRLPAEFEIT+YCHPCGSKSKNVLAVQVL+WSDGSYLED
Sbjct: 181  EAVDSAFFVWINGSLVGYSQDSRLPAEFEITKYCHPCGSKSKNVLAVQVLRWSDGSYLED 240

Query: 265  QDQWWLSGIHRDVLLLSKPQVFIGDYFFKSCVGEDFSYADIQVEVKIDNSLDTSKENFLN 324
            QDQWWLSGIHRDVLLLSKPQVFIGDYFFKS VGEDFS+ADIQVEVKIDNSL+T KENFLN
Sbjct: 241  QDQWWLSGIHRDVLLLSKPQVFIGDYFFKSHVGEDFSWADIQVEVKIDNSLETKKENFLN 300

Query: 325  DFKLEAILFDNRTWDNHEGQADLLSSNVANVKLTLLSATTLGFHGYVLGGRLEKPKLWSA 384
            DFKLEA+LFDN T DNHEG ADLLSS+VA+VKL+LLSATTLGFHGYVLGGR+EKPKLWSA
Sbjct: 301  DFKLEAVLFDNGTLDNHEGIADLLSSSVASVKLSLLSATTLGFHGYVLGGRMEKPKLWSA 360

Query: 385  EHPQLYTLIILLKDLSDQIVDCESCLVGIRSITKAPKQLLVNGCPVVIRGVNRHEHHPRL 444
            E P LYTLI+LLKD SD+I+DCESCLVGIRSITKAPKQLLVNGCPVVIRGVNRHEHHPRL
Sbjct: 361  EQPHLYTLIVLLKDSSDKIIDCESCLVGIRSITKAPKQLLVNGCPVVIRGVNRHEHHPRL 420

Query: 445  GKTNIESCMVQDLVLMKQNNMNAVRNSHYPQHPRWYELCDLFGMYMIDEANIETHGFDYT 504
            GKTNIESCMV+DLVLMKQNN+NAVRNSHYPQHPRWYELCDLFGMYM+DEANIETHGFD +
Sbjct: 421  GKTNIESCMVKDLVLMKQNNINAVRNSHYPQHPRWYELCDLFGMYMVDEANIETHGFDLS 480

Query: 505  GHVKHPTLQPSWAAAMLDRVIGMVERDKNHACIIAWSLGNEAGYGPNHSALAGWIRGKDS 564
            GHVKHPTLQPSWAAAMLDRVIGMVERDKNHACII WSLGNE+GYGPNHSALAGWIRGKD 
Sbjct: 481  GHVKHPTLQPSWAAAMLDRVIGMVERDKNHACIIVWSLGNESGYGPNHSALAGWIRGKDP 540

Query: 565  SRVLHYEGGGSRTSSTDIICPMYMRVWDMVKIANDPNETRPLILCEYSHSMGNSTGNLHK 624
            SRVLHYEGGGSRTSSTDIICPMYMRVWD+VKIANDP+ETRPLILCEYSHSMGNSTGNLHK
Sbjct: 541  SRVLHYEGGGSRTSSTDIICPMYMRVWDIVKIANDPDETRPLILCEYSHSMGNSTGNLHK 600

Query: 625  YWEAIDNTFGLQGGFIWDWVDQALLKEVDNGRKRWAYGGEFGDVPNDSNFCLNGVTWPDR 684
            YWEAIDNTFGLQGGFIWDWVDQALLKEV NGRKRWAYGGEFGD+PNDS FC+NGVTWPDR
Sbjct: 601  YWEAIDNTFGLQGGFIWDWVDQALLKEVGNGRKRWAYGGEFGDIPNDSVFCMNGVTWPDR 660

Query: 685  TPHPALHEVKYLYQPIKISSKDGALQVLNAHFFSTTEDLEFSWSIYGDGLELGNGILSLP 744
            TPHPAL+EVK LYQ IK S KDG L+VLNAHFF+TTEDLEFSWSIYGDGLELGNGILSLP
Sbjct: 661  TPHPALNEVKNLYQEIKFSFKDGTLKVLNAHFFTTTEDLEFSWSIYGDGLELGNGILSLP 720

Query: 745  VIGPQESYNIEWQTSPWYDLWASSPALEFFLNVSVKLLHSTRWVEAGHIISSSQVQLPMK 804
            V+GPQ ++NIEWQ+SPWYDLWASSPALEFFL VSVK LHSTRWV AGH ISSSQVQLPMK
Sbjct: 721  VLGPQGTHNIEWQSSPWYDLWASSPALEFFLTVSVKRLHSTRWVNAGHTISSSQVQLPMK 780

Query: 805  REFFPHSIKNGSASLLSEILGDTVRVYQQNLWEIKLDVQTGTLESWKVKGVPLIIKGIVP 864
            REFFPHSIKN +++LLSEILGDTVRVYQQ+LWEIKLDVQTGTLESWKVKGVPLIIKGI+P
Sbjct: 781  REFFPHSIKNENSTLLSEILGDTVRVYQQDLWEIKLDVQTGTLESWKVKGVPLIIKGIMP 840

Query: 865  SFWRAPTDNDKGGGSCSYLSLWKAAHIDSLSFTAERCSILSTTEHYVKIAVVFLGLENDE 924
            SFWRAPTDNDKGGGSCSY SLWKAAHIDSLSFTAERCSILSTTEHYVK+A+VFLG+ +D+
Sbjct: 841  SFWRAPTDNDKGGGSCSYFSLWKAAHIDSLSFTAERCSILSTTEHYVKVAIVFLGVGSDD 900

Query: 925  QQASFSDSEKSNVLIQVDMTYTIYGSGDVIVDCNVQPSPNLPPLPRVGVEFHLDKSVDQV 984
            QQAS SDSEKSN+LIQVDMTYTI+GSGDVIVDC VQPSPNLPPLPRVGV+F+LDKS+D+V
Sbjct: 901  QQASSSDSEKSNILIQVDMTYTIFGSGDVIVDCKVQPSPNLPPLPRVGVKFNLDKSMDRV 960

Query: 985  RWYGRGPFECYPDRKAAAHVGVYEKNVSEMHVPYIVPGESSGRADVRWVTFENKDGVGIY 1044
            RWYGRGPFECYPDRKAAAHVGVYEKNVSEMHVPYIVPGESSGR DVRWVT ENKDG+GIY
Sbjct: 961  RWYGRGPFECYPDRKAAAHVGVYEKNVSEMHVPYIVPGESSGRTDVRWVTLENKDGIGIY 1020

Query: 1045 ASIYRGSPPMQMSASYYSTEELDRAVHNEDLVEGDDIEVHLDHKHMGVGGDDSWSPCVHD 1104
            ASIY  SPPMQMSASYYS+ ELDRA HNE+LVEGDDIEVHLDHKHMGVGGDDSWSPCVHD
Sbjct: 1021 ASIYGSSPPMQMSASYYSSAELDRAEHNEELVEGDDIEVHLDHKHMGVGGDDSWSPCVHD 1080

Query: 1105 EYLLSPVPYSFSIRFYPLTPSASGYDAYKSQLPL 1139
            EYLL PVPYSFSIRF P+TPS S Y+AY+SQLP+
Sbjct: 1081 EYLLPPVPYSFSIRFCPVTPSTSAYEAYRSQLPV 1114

BLAST of Spg009799 vs. ExPASy TrEMBL
Match: A0A5D3CBT7 (Lactase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G003100 PE=3 SV=1)

HSP 1 Score: 2185.6 bits (5662), Expect = 0.0e+00
Identity = 1006/1114 (90.31%), Postives = 1064/1114 (95.51%), Query Frame = 0

Query: 25   MASLAAKLMIPSENGYRVWEDQNFIKWKKRDSHVPLRCQDSVEGCLKYWHDRSKVDFLVS 84
            MA+LA+KLMIPS+NGYRVWEDQ FIKW+KRDSHVPLRCQDSVEGCLKYW DR+KVDFLVS
Sbjct: 1    MAALASKLMIPSDNGYRVWEDQTFIKWRKRDSHVPLRCQDSVEGCLKYWQDRTKVDFLVS 60

Query: 85   NSAVWNDDAVQSALDCAAFWVKGLPFIKSLSGYWKFYLAATPTSVPENFHGSAFEDSEWT 144
            N AVWNDDAVQSALD AAFWVK LPFIKSLSGYWKFYLAATPTSVP NFH + FEDS+W+
Sbjct: 61   NLAVWNDDAVQSALDSAAFWVKDLPFIKSLSGYWKFYLAATPTSVPHNFHATVFEDSQWS 120

Query: 145  NLPVPSNWQMHGFDRPIYTNVVYPFPLDPPHVPEDNPTGCYRTYFQLPEEWKGRRILLHF 204
            NLPVPSNWQMHGFDRPIYTNVVYPFPLDPPHVPEDNPTGCYRTYF LPEEWKGRRILLHF
Sbjct: 121  NLPVPSNWQMHGFDRPIYTNVVYPFPLDPPHVPEDNPTGCYRTYFHLPEEWKGRRILLHF 180

Query: 205  EAVDCAFFVWINGSLVGYSQDSRLPAEFEITEYCHPCGSKSKNVLAVQVLKWSDGSYLED 264
            EAVD AFF WINGSLVGYSQDSRLPAEFEITEYCHPCGS+SKNVLAVQVLKWSDGSYLED
Sbjct: 181  EAVDSAFFAWINGSLVGYSQDSRLPAEFEITEYCHPCGSQSKNVLAVQVLKWSDGSYLED 240

Query: 265  QDQWWLSGIHRDVLLLSKPQVFIGDYFFKSCVGEDFSYADIQVEVKIDNSLDTSKENFLN 324
            QDQWWLSGIHRDV+LLSKPQVFIGDYFFKS VGEDFSYADIQVEVKIDNSL+T KENFLN
Sbjct: 241  QDQWWLSGIHRDVILLSKPQVFIGDYFFKSHVGEDFSYADIQVEVKIDNSLETRKENFLN 300

Query: 325  DFKLEAILFDNRTWDNHEGQADLLSSNVANVKLTLLSATTLGFHGYVLGGRLEKPKLWSA 384
            DFKLEA+LFDN TWDNH+G  DLLSS++ANVKL+LLS TTLGFHGYVLGGRLEKPKLWSA
Sbjct: 301  DFKLEAVLFDNGTWDNHDGNVDLLSSSMANVKLSLLSVTTLGFHGYVLGGRLEKPKLWSA 360

Query: 385  EHPQLYTLIILLKDLSDQIVDCESCLVGIRSITKAPKQLLVNGCPVVIRGVNRHEHHPRL 444
            E P LYTL++LLKD SDQIVDCESCLVGIRSITK PKQLLVNGCPVVIRGVNRHEHHPRL
Sbjct: 361  EQPHLYTLVVLLKDSSDQIVDCESCLVGIRSITKGPKQLLVNGCPVVIRGVNRHEHHPRL 420

Query: 445  GKTNIESCMVQDLVLMKQNNMNAVRNSHYPQHPRWYELCDLFGMYMIDEANIETHGFDYT 504
            GKTNIESCMVQDLVLMKQ+N+NAVRNSHYPQH RWYELCDLFGMYM+DEANIETHGFD++
Sbjct: 421  GKTNIESCMVQDLVLMKQHNINAVRNSHYPQHSRWYELCDLFGMYMVDEANIETHGFDFS 480

Query: 505  GHVKHPTLQPSWAAAMLDRVIGMVERDKNHACIIAWSLGNEAGYGPNHSALAGWIRGKDS 564
            GHVKHPTLQPSWAAAMLDRVIGMVERDKNHACII WSLGNE+GYGPNH ALAGWIRGKDS
Sbjct: 481  GHVKHPTLQPSWAAAMLDRVIGMVERDKNHACIIVWSLGNESGYGPNHFALAGWIRGKDS 540

Query: 565  SRVLHYEGGGSRTSSTDIICPMYMRVWDMVKIANDPNETRPLILCEYSHSMGNSTGNLHK 624
            SRVLHYEGGGSRTSSTDIICPMYMRVWD+VKIANDPNETRPLILCEYSHSMGNSTGNLHK
Sbjct: 541  SRVLHYEGGGSRTSSTDIICPMYMRVWDIVKIANDPNETRPLILCEYSHSMGNSTGNLHK 600

Query: 625  YWEAIDNTFGLQGGFIWDWVDQALLKEVDNGRKRWAYGGEFGDVPNDSNFCLNGVTWPDR 684
            YWEAIDNTFGLQGGFIWDWVDQALLKEV NGRKRWAYGGEFGD+PNDS FCLNGVTWPDR
Sbjct: 601  YWEAIDNTFGLQGGFIWDWVDQALLKEVGNGRKRWAYGGEFGDIPNDSTFCLNGVTWPDR 660

Query: 685  TPHPALHEVKYLYQPIKISSKDGALQVLNAHFFSTTEDLEFSWSIYGDGLELGNGILSLP 744
            TPHPALHEVKYL+Q IKISSKDG L+VLN HFFSTTEDLEFSWSIYGDGLELGNGILSLP
Sbjct: 661  TPHPALHEVKYLHQAIKISSKDGTLKVLNGHFFSTTEDLEFSWSIYGDGLELGNGILSLP 720

Query: 745  VIGPQESYNIEWQTSPWYDLWASSPALEFFLNVSVKLLHSTRWVEAGHIISSSQVQLPMK 804
            V+GPQ SYNIEWQ+SPWYDLWASS ALEFFL +SVKLLHSTRW EAGH +S SQVQLPMK
Sbjct: 721  VLGPQGSYNIEWQSSPWYDLWASSHALEFFLTISVKLLHSTRWAEAGHTVSLSQVQLPMK 780

Query: 805  REFFPHSIKNGSASLLSEILGDTVRVYQQNLWEIKLDVQTGTLESWKVKGVPLIIKGIVP 864
            REFFPHSIKNG+++L++EI+GDTVRVYQQNLWEIKLDVQTGTLESWKVKGVPLIIKGI+P
Sbjct: 781  REFFPHSIKNGNSTLVNEIVGDTVRVYQQNLWEIKLDVQTGTLESWKVKGVPLIIKGIIP 840

Query: 865  SFWRAPTDNDKGGGSCSYLSLWKAAHIDSLSFTAERCSILSTTEHYVKIAVVFLGLENDE 924
            SFWRAPTDNDKGGGSCSYLS+WKAAHID+LSFTAERCSILSTTEHYVKIAVVFLG+ +D+
Sbjct: 841  SFWRAPTDNDKGGGSCSYLSVWKAAHIDNLSFTAERCSILSTTEHYVKIAVVFLGVGSDD 900

Query: 925  QQASFSDSEKSNVLIQVDMTYTIYGSGDVIVDCNVQPSPNLPPLPRVGVEFHLDKSVDQV 984
            +QAS SD EKSNVLIQ DMTYTI+GSGDV+VDCNVQPS NLPP PRVGV+FHLDKS+D+V
Sbjct: 901  RQASNSDLEKSNVLIQADMTYTIFGSGDVLVDCNVQPSSNLPPFPRVGVKFHLDKSMDRV 960

Query: 985  RWYGRGPFECYPDRKAAAHVGVYEKNVSEMHVPYIVPGESSGRADVRWVTFENKDGVGIY 1044
            +WYGRGPFECYPDRKAAAHVGVYEKNVSEMHVPYIVPGESSGR DVRWVTFENKDGVGIY
Sbjct: 961  KWYGRGPFECYPDRKAAAHVGVYEKNVSEMHVPYIVPGESSGRTDVRWVTFENKDGVGIY 1020

Query: 1045 ASIYRGSPPMQMSASYYSTEELDRAVHNEDLVEGDDIEVHLDHKHMGVGGDDSWSPCVHD 1104
            ASIY  SPPMQM ASYYST +L+RA HN+DLVEGDDIEV+LDHKHMGVGGDDSWSPCVH+
Sbjct: 1021 ASIYGNSPPMQMRASYYSTADLERAGHNDDLVEGDDIEVNLDHKHMGVGGDDSWSPCVHE 1080

Query: 1105 EYLLSPVPYSFSIRFYPLTPSASGYDAYKSQLPL 1139
            EYLL PVPYSFSIRFYP+TPS SGYDAY+SQL L
Sbjct: 1081 EYLLPPVPYSFSIRFYPVTPSTSGYDAYRSQLLL 1114

BLAST of Spg009799 vs. ExPASy TrEMBL
Match: A0A1S3BGB3 (Lactase OS=Cucumis melo OX=3656 GN=LOC103489515 PE=3 SV=1)

HSP 1 Score: 2185.6 bits (5662), Expect = 0.0e+00
Identity = 1006/1114 (90.31%), Postives = 1064/1114 (95.51%), Query Frame = 0

Query: 25   MASLAAKLMIPSENGYRVWEDQNFIKWKKRDSHVPLRCQDSVEGCLKYWHDRSKVDFLVS 84
            MA+LA+KLMIPS+NGYRVWEDQ FIKW+KRDSHVPLRCQDSVEGCLKYW DR+KVDFLVS
Sbjct: 1    MAALASKLMIPSDNGYRVWEDQTFIKWRKRDSHVPLRCQDSVEGCLKYWQDRTKVDFLVS 60

Query: 85   NSAVWNDDAVQSALDCAAFWVKGLPFIKSLSGYWKFYLAATPTSVPENFHGSAFEDSEWT 144
            N AVWNDDAVQSALD AAFWVK LPFIKSLSGYWKFYLAATPTSVP NFH + FEDS+W+
Sbjct: 61   NLAVWNDDAVQSALDSAAFWVKDLPFIKSLSGYWKFYLAATPTSVPHNFHATVFEDSQWS 120

Query: 145  NLPVPSNWQMHGFDRPIYTNVVYPFPLDPPHVPEDNPTGCYRTYFQLPEEWKGRRILLHF 204
            NLPVPSNWQMHGFDRPIYTNVVYPFPLDPPHVPEDNPTGCYRTYF LPEEWKGRRILLHF
Sbjct: 121  NLPVPSNWQMHGFDRPIYTNVVYPFPLDPPHVPEDNPTGCYRTYFHLPEEWKGRRILLHF 180

Query: 205  EAVDCAFFVWINGSLVGYSQDSRLPAEFEITEYCHPCGSKSKNVLAVQVLKWSDGSYLED 264
            EAVD AFF WINGSLVGYSQDSRLPAEFEITEYCHPCGS+SKNVLAVQVLKWSDGSYLED
Sbjct: 181  EAVDSAFFAWINGSLVGYSQDSRLPAEFEITEYCHPCGSQSKNVLAVQVLKWSDGSYLED 240

Query: 265  QDQWWLSGIHRDVLLLSKPQVFIGDYFFKSCVGEDFSYADIQVEVKIDNSLDTSKENFLN 324
            QDQWWLSGIHRDV+LLSKPQVFIGDYFFKS VGEDFSYADIQVEVKIDNSL+T KENFLN
Sbjct: 241  QDQWWLSGIHRDVILLSKPQVFIGDYFFKSHVGEDFSYADIQVEVKIDNSLETRKENFLN 300

Query: 325  DFKLEAILFDNRTWDNHEGQADLLSSNVANVKLTLLSATTLGFHGYVLGGRLEKPKLWSA 384
            DFKLEA+LFDN TWDNH+G  DLLSS++ANVKL+LLS TTLGFHGYVLGGRLEKPKLWSA
Sbjct: 301  DFKLEAVLFDNGTWDNHDGNVDLLSSSMANVKLSLLSVTTLGFHGYVLGGRLEKPKLWSA 360

Query: 385  EHPQLYTLIILLKDLSDQIVDCESCLVGIRSITKAPKQLLVNGCPVVIRGVNRHEHHPRL 444
            E P LYTL++LLKD SDQIVDCESCLVGIRSITK PKQLLVNGCPVVIRGVNRHEHHPRL
Sbjct: 361  EQPHLYTLVVLLKDSSDQIVDCESCLVGIRSITKGPKQLLVNGCPVVIRGVNRHEHHPRL 420

Query: 445  GKTNIESCMVQDLVLMKQNNMNAVRNSHYPQHPRWYELCDLFGMYMIDEANIETHGFDYT 504
            GKTNIESCMVQDLVLMKQ+N+NAVRNSHYPQH RWYELCDLFGMYM+DEANIETHGFD++
Sbjct: 421  GKTNIESCMVQDLVLMKQHNINAVRNSHYPQHSRWYELCDLFGMYMVDEANIETHGFDFS 480

Query: 505  GHVKHPTLQPSWAAAMLDRVIGMVERDKNHACIIAWSLGNEAGYGPNHSALAGWIRGKDS 564
            GHVKHPTLQPSWAAAMLDRVIGMVERDKNHACII WSLGNE+GYGPNH ALAGWIRGKDS
Sbjct: 481  GHVKHPTLQPSWAAAMLDRVIGMVERDKNHACIIVWSLGNESGYGPNHFALAGWIRGKDS 540

Query: 565  SRVLHYEGGGSRTSSTDIICPMYMRVWDMVKIANDPNETRPLILCEYSHSMGNSTGNLHK 624
            SRVLHYEGGGSRTSSTDIICPMYMRVWD+VKIANDPNETRPLILCEYSHSMGNSTGNLHK
Sbjct: 541  SRVLHYEGGGSRTSSTDIICPMYMRVWDIVKIANDPNETRPLILCEYSHSMGNSTGNLHK 600

Query: 625  YWEAIDNTFGLQGGFIWDWVDQALLKEVDNGRKRWAYGGEFGDVPNDSNFCLNGVTWPDR 684
            YWEAIDNTFGLQGGFIWDWVDQALLKEV NGRKRWAYGGEFGD+PNDS FCLNGVTWPDR
Sbjct: 601  YWEAIDNTFGLQGGFIWDWVDQALLKEVGNGRKRWAYGGEFGDIPNDSTFCLNGVTWPDR 660

Query: 685  TPHPALHEVKYLYQPIKISSKDGALQVLNAHFFSTTEDLEFSWSIYGDGLELGNGILSLP 744
            TPHPALHEVKYL+Q IKISSKDG L+VLN HFFSTTEDLEFSWSIYGDGLELGNGILSLP
Sbjct: 661  TPHPALHEVKYLHQAIKISSKDGTLKVLNGHFFSTTEDLEFSWSIYGDGLELGNGILSLP 720

Query: 745  VIGPQESYNIEWQTSPWYDLWASSPALEFFLNVSVKLLHSTRWVEAGHIISSSQVQLPMK 804
            V+GPQ SYNIEWQ+SPWYDLWASS ALEFFL +SVKLLHSTRW EAGH +S SQVQLPMK
Sbjct: 721  VLGPQGSYNIEWQSSPWYDLWASSHALEFFLTISVKLLHSTRWAEAGHTVSLSQVQLPMK 780

Query: 805  REFFPHSIKNGSASLLSEILGDTVRVYQQNLWEIKLDVQTGTLESWKVKGVPLIIKGIVP 864
            REFFPHSIKNG+++L++EI+GDTVRVYQQNLWEIKLDVQTGTLESWKVKGVPLIIKGI+P
Sbjct: 781  REFFPHSIKNGNSTLVNEIVGDTVRVYQQNLWEIKLDVQTGTLESWKVKGVPLIIKGIIP 840

Query: 865  SFWRAPTDNDKGGGSCSYLSLWKAAHIDSLSFTAERCSILSTTEHYVKIAVVFLGLENDE 924
            SFWRAPTDNDKGGGSCSYLS+WKAAHID+LSFTAERCSILSTTEHYVKIAVVFLG+ +D+
Sbjct: 841  SFWRAPTDNDKGGGSCSYLSVWKAAHIDNLSFTAERCSILSTTEHYVKIAVVFLGVGSDD 900

Query: 925  QQASFSDSEKSNVLIQVDMTYTIYGSGDVIVDCNVQPSPNLPPLPRVGVEFHLDKSVDQV 984
            +QAS SD EKSNVLIQ DMTYTI+GSGDV+VDCNVQPS NLPP PRVGV+FHLDKS+D+V
Sbjct: 901  RQASNSDLEKSNVLIQADMTYTIFGSGDVLVDCNVQPSSNLPPFPRVGVKFHLDKSMDRV 960

Query: 985  RWYGRGPFECYPDRKAAAHVGVYEKNVSEMHVPYIVPGESSGRADVRWVTFENKDGVGIY 1044
            +WYGRGPFECYPDRKAAAHVGVYEKNVSEMHVPYIVPGESSGR DVRWVTFENKDGVGIY
Sbjct: 961  KWYGRGPFECYPDRKAAAHVGVYEKNVSEMHVPYIVPGESSGRTDVRWVTFENKDGVGIY 1020

Query: 1045 ASIYRGSPPMQMSASYYSTEELDRAVHNEDLVEGDDIEVHLDHKHMGVGGDDSWSPCVHD 1104
            ASIY  SPPMQM ASYYST +L+RA HN+DLVEGDDIEV+LDHKHMGVGGDDSWSPCVH+
Sbjct: 1021 ASIYGNSPPMQMRASYYSTADLERAGHNDDLVEGDDIEVNLDHKHMGVGGDDSWSPCVHE 1080

Query: 1105 EYLLSPVPYSFSIRFYPLTPSASGYDAYKSQLPL 1139
            EYLL PVPYSFSIRFYP+TPS SGYDAY+SQL L
Sbjct: 1081 EYLLPPVPYSFSIRFYPVTPSTSGYDAYRSQLLL 1114

BLAST of Spg009799 vs. ExPASy TrEMBL
Match: A0A6J1CDH4 (Lactase OS=Momordica charantia OX=3673 GN=LOC111010223 PE=3 SV=1)

HSP 1 Score: 2181.0 bits (5650), Expect = 0.0e+00
Identity = 1006/1116 (90.14%), Postives = 1063/1116 (95.25%), Query Frame = 0

Query: 25   MASLAAKLMIPSENGYRVWEDQNFIKWKKRDSHVPLRCQDSVEGCLKYWHDRSKVDFLVS 84
            MASLAAKL+IPSENGY+VWEDQNFIKW+KRDSHVPLRCQDSVEGC+KYW DRSKVDFLVS
Sbjct: 1    MASLAAKLVIPSENGYKVWEDQNFIKWRKRDSHVPLRCQDSVEGCIKYWQDRSKVDFLVS 60

Query: 85   NSAVWNDDAVQSALDCAAFWVKGLPFIKSLSGYWKFYLAATPTSVPENFHGSAFEDSEWT 144
             SAVWNDDAV SALDCAAFWVK LPFIKSLSGYWKF+LAA+PTSVP +FH S+FEDSEWT
Sbjct: 61   KSAVWNDDAVHSALDCAAFWVKDLPFIKSLSGYWKFFLAASPTSVPVDFHESSFEDSEWT 120

Query: 145  NLPVPSNWQMHGFDRPIYTNVVYPFPLDPPHVPEDNPTGCYRTYFQLPEEWKGRRILLHF 204
             LPVPSNWQMHGFD+PIYTNVVYPFP+DPPHVPEDNPTGCYRTYF LPEEWKGRRILLHF
Sbjct: 121  TLPVPSNWQMHGFDKPIYTNVVYPFPVDPPHVPEDNPTGCYRTYFHLPEEWKGRRILLHF 180

Query: 205  EAVDCAFFVWINGSLVGYSQDSRLPAEFEITEYCHPCGSKSKNVLAVQVLKWSDGSYLED 264
            EAVD AFFVWING+LVGYSQDSRLPAEFEITEYCHPCGSKSKNV+AVQV+KWSDGSYLED
Sbjct: 181  EAVDSAFFVWINGNLVGYSQDSRLPAEFEITEYCHPCGSKSKNVVAVQVIKWSDGSYLED 240

Query: 265  QDQWWLSGIHRDVLLLSKPQVFIGDYFFKSCVGEDFSYADIQVEVKIDNSLDTSKENFLN 324
            QDQWWLSGIHRDVLL++KPQVFIGDYFFKS  GED+SYADIQVEVKID+SL+ SKENFLN
Sbjct: 241  QDQWWLSGIHRDVLLMAKPQVFIGDYFFKSKAGEDYSYADIQVEVKIDSSLEASKENFLN 300

Query: 325  DFKLEAILFDNRTWD--NHEGQADLLSSNVANVKLTLLSATTLGFHGYVLGGRLEKPKLW 384
            DFKLEA+LFD+ TWD  NHE   DLLSSNVANVKLTLL  T LGFHGYVLGGRLEKP+LW
Sbjct: 301  DFKLEAVLFDSGTWDNNNHESHVDLLSSNVANVKLTLLPGTALGFHGYVLGGRLEKPRLW 360

Query: 385  SAEHPQLYTLIILLKDLSDQIVDCESCLVGIRSITKAPKQLLVNGCPVVIRGVNRHEHHP 444
            SAE P LYTL++LLKD SD+IVDCESCLVGIRSI KAPKQLLVNGCPVVIRGVNRHEHHP
Sbjct: 361  SAEQPNLYTLVVLLKDSSDRIVDCESCLVGIRSIRKAPKQLLVNGCPVVIRGVNRHEHHP 420

Query: 445  RLGKTNIESCMVQDLVLMKQNNMNAVRNSHYPQHPRWYELCDLFGMYMIDEANIETHGFD 504
            RLGKTNIESCM+QDLVLMKQNN+NAVRNSHYPQHPRWYELCDLFGMYMIDEANIETHGFD
Sbjct: 421  RLGKTNIESCMIQDLVLMKQNNINAVRNSHYPQHPRWYELCDLFGMYMIDEANIETHGFD 480

Query: 505  YTGHVKHPTLQPSWAAAMLDRVIGMVERDKNHACIIAWSLGNEAGYGPNHSALAGWIRGK 564
             +GHVKHPTL+PSWA+AMLDRVIGMVERDKNHACIIAWSLGNEAGYGPNHSALAGWIRGK
Sbjct: 481  LSGHVKHPTLEPSWASAMLDRVIGMVERDKNHACIIAWSLGNEAGYGPNHSALAGWIRGK 540

Query: 565  DSSRVLHYEGGGSRTSSTDIICPMYMRVWDMVKIANDPNETRPLILCEYSHSMGNSTGNL 624
            DSSRVLHYEGGGSRTSSTDI+CPMYMRVWDMVKIANDPNETRPLILCEYSHSMGNSTGNL
Sbjct: 541  DSSRVLHYEGGGSRTSSTDIVCPMYMRVWDMVKIANDPNETRPLILCEYSHSMGNSTGNL 600

Query: 625  HKYWEAIDNTFGLQGGFIWDWVDQALLKEVDNGRKRWAYGGEFGDVPNDSNFCLNGVTWP 684
            H+YWEAIDNTFGLQGGFIWDWVDQALLKEV +GRKRWAYGGEFGD+PND NFCLNGVTWP
Sbjct: 601  HRYWEAIDNTFGLQGGFIWDWVDQALLKEVGDGRKRWAYGGEFGDIPNDLNFCLNGVTWP 660

Query: 685  DRTPHPALHEVKYLYQPIKISSKDGALQVLNAHFFSTTEDLEFSWSIYGDGLELGNGILS 744
            DRTPHPALHEVKYLYQPIKISS+DG LQVLN HFFSTTEDLEFSW IYGDG ELGNG LS
Sbjct: 661  DRTPHPALHEVKYLYQPIKISSRDGTLQVLNGHFFSTTEDLEFSWRIYGDGHELGNGTLS 720

Query: 745  LPVIGPQESYNIEWQTSPWYDLWASSPALEFFLNVSVKLLHSTRWVEAGHIISSSQVQLP 804
            LPV+GPQ SY+IEWQ+SPWYDLWASSPALEFFL VSVKL   TRWVEAGHI+S SQVQLP
Sbjct: 721  LPVLGPQRSYDIEWQSSPWYDLWASSPALEFFLTVSVKLQRPTRWVEAGHIVSLSQVQLP 780

Query: 805  MKREFFPHSIKNGSASLLSEILGDTVRVYQQNLWEIKLDVQTGTLESWKVKGVPLIIKGI 864
             KR+FFPHSIKN SA+LLSEI GDTVRVYQQNLWEIKLDVQTGTLESWKVKGVPLIIKGI
Sbjct: 781  TKRDFFPHSIKNVSATLLSEIHGDTVRVYQQNLWEIKLDVQTGTLESWKVKGVPLIIKGI 840

Query: 865  VPSFWRAPTDNDKGGGSCSYLSLWKAAHIDSLSFTAERCSILSTTEHYVKIAVVFLGLEN 924
            +PSFWRAPTDNDKGGGSCSYLSLWKAAHID+LSFTAERCSILSTT+H+VK+AV+FLG+ +
Sbjct: 841  IPSFWRAPTDNDKGGGSCSYLSLWKAAHIDTLSFTAERCSILSTTDHFVKVAVIFLGVGS 900

Query: 925  DEQQASFSDSEKSNVLIQVDMTYTIYGSGDVIVDCNVQPSPNLPPLPRVGVEFHLDKSVD 984
            D Q AS S SEKS+ LIQVDM YTIYGSGDVIVDCNVQPSPNLPPLPRVGVEFHLDKSVD
Sbjct: 901  DRQWASSSASEKSDALIQVDMIYTIYGSGDVIVDCNVQPSPNLPPLPRVGVEFHLDKSVD 960

Query: 985  QVRWYGRGPFECYPDRKAAAHVGVYEKNVSEMHVPYIVPGESSGRADVRWVTFENKDGVG 1044
            +VRWYGRGPFECYPDRKAAAHVGVYEKNVSEMHVPYIVPGESSGRAD+RWVTFENKDGVG
Sbjct: 961  RVRWYGRGPFECYPDRKAAAHVGVYEKNVSEMHVPYIVPGESSGRADIRWVTFENKDGVG 1020

Query: 1045 IYASIYRGSPPMQMSASYYSTEELDRAVHNEDLVEGDDIEVHLDHKHMGVGGDDSWSPCV 1104
            IYASIYR SPPMQMSASYYST ELDRAVHN+DLV GDDIEVHLDH+HMGVGGDDSWSPCV
Sbjct: 1021 IYASIYRSSPPMQMSASYYSTAELDRAVHNDDLVNGDDIEVHLDHQHMGVGGDDSWSPCV 1080

Query: 1105 HDEYLLSPVPYSFSIRFYPLTPSASGYDAYKSQLPL 1139
            HD+YLLSPV YSFS+RFYP+TPSASG+DAYKSQLPL
Sbjct: 1081 HDDYLLSPVAYSFSMRFYPITPSASGHDAYKSQLPL 1116

BLAST of Spg009799 vs. ExPASy TrEMBL
Match: A0A6J1JEA6 (Lactase OS=Cucurbita maxima OX=3661 GN=LOC111486104 PE=3 SV=1)

HSP 1 Score: 2178.3 bits (5643), Expect = 0.0e+00
Identity = 1007/1114 (90.39%), Postives = 1066/1114 (95.69%), Query Frame = 0

Query: 25   MASLAAKLMIPSENGYRVWEDQNFIKWKKRDSHVPLRCQDSVEGCLKYWHDRSKVDFLVS 84
            MA L AKL+IPS+NGY+VWED NFIKWKKRDSHVPLRCQDSVEGCLKYWHDRSKVDFLVS
Sbjct: 1    MAVLGAKLVIPSDNGYKVWEDPNFIKWKKRDSHVPLRCQDSVEGCLKYWHDRSKVDFLVS 60

Query: 85   NSAVWNDDAVQSALDCAAFWVKGLPFIKSLSGYWKFYLAATPTSVPENFHGSAFEDSEWT 144
            NSAVWNDDAVQSALD AAFWVKGLPFIKSLSGYWKFYLAA+PTSVPE+FHGSAFEDSEWT
Sbjct: 61   NSAVWNDDAVQSALDSAAFWVKGLPFIKSLSGYWKFYLAASPTSVPEDFHGSAFEDSEWT 120

Query: 145  NLPVPSNWQMHGFDRPIYTNVVYPFPLDPPHVPEDNPTGCYRTYFQLPEEWKGRRILLHF 204
             LPVPSNWQMHGFDRPIYTN+VYPFPLDPPHVPEDNPTGCYRTYF LPEEWKGRRILLHF
Sbjct: 121  TLPVPSNWQMHGFDRPIYTNIVYPFPLDPPHVPEDNPTGCYRTYFHLPEEWKGRRILLHF 180

Query: 205  EAVDCAFFVWINGSLVGYSQDSRLPAEFEITEYCHPCGSKSKNVLAVQVLKWSDGSYLED 264
            EAVD AFFVWINGSLVGYSQDSRLPAEFEITEYCHPCGSKSKNVLAVQVL+WSDGSYLED
Sbjct: 181  EAVDSAFFVWINGSLVGYSQDSRLPAEFEITEYCHPCGSKSKNVLAVQVLRWSDGSYLED 240

Query: 265  QDQWWLSGIHRDVLLLSKPQVFIGDYFFKSCVGEDFSYADIQVEVKIDNSLDTSKENFLN 324
            QDQWWLSGIHRDVLLLSKPQVFIGDYFFKS VGEDFS+ADIQVEVKIDNSL+T KENFL 
Sbjct: 241  QDQWWLSGIHRDVLLLSKPQVFIGDYFFKSHVGEDFSWADIQVEVKIDNSLETKKENFLY 300

Query: 325  DFKLEAILFDNRTWDNHEGQADLLSSNVANVKLTLLSATTLGFHGYVLGGRLEKPKLWSA 384
            D+KLEA+LFD  T DNHE  ADLLSSNVA+VKL+LLSATTLGFHGYVLGGR+EKPKLWSA
Sbjct: 301  DYKLEAVLFDVGTLDNHEDIADLLSSNVASVKLSLLSATTLGFHGYVLGGRMEKPKLWSA 360

Query: 385  EHPQLYTLIILLKDLSDQIVDCESCLVGIRSITKAPKQLLVNGCPVVIRGVNRHEHHPRL 444
            E P LYTLI+LLKD SD+I+DCESCLVGIRSITKAPKQLLVNGCPVVIRGVNRHEHHPRL
Sbjct: 361  EQPHLYTLIVLLKDSSDKIIDCESCLVGIRSITKAPKQLLVNGCPVVIRGVNRHEHHPRL 420

Query: 445  GKTNIESCMVQDLVLMKQNNMNAVRNSHYPQHPRWYELCDLFGMYMIDEANIETHGFDYT 504
            GKTNIESCMV+DLVLMKQNN+NAVRNSHYPQHPRWYELCDLFGMYM+DEANIETHGFD +
Sbjct: 421  GKTNIESCMVKDLVLMKQNNINAVRNSHYPQHPRWYELCDLFGMYMVDEANIETHGFDLS 480

Query: 505  GHVKHPTLQPSWAAAMLDRVIGMVERDKNHACIIAWSLGNEAGYGPNHSALAGWIRGKDS 564
            GHVKHPTLQPSWAAAMLDRVIGMVERDKNHACII WSLGNE+GYGPNHSALAGWIRGKD 
Sbjct: 481  GHVKHPTLQPSWAAAMLDRVIGMVERDKNHACIIVWSLGNESGYGPNHSALAGWIRGKDP 540

Query: 565  SRVLHYEGGGSRTSSTDIICPMYMRVWDMVKIANDPNETRPLILCEYSHSMGNSTGNLHK 624
            SRVLHYEGGGSRTSSTDIICPMYMRVWD+VKIANDP+ETRPLILCEYSHSMGNSTGNLHK
Sbjct: 541  SRVLHYEGGGSRTSSTDIICPMYMRVWDIVKIANDPDETRPLILCEYSHSMGNSTGNLHK 600

Query: 625  YWEAIDNTFGLQGGFIWDWVDQALLKEVDNGRKRWAYGGEFGDVPNDSNFCLNGVTWPDR 684
            YWEAIDNTFGLQGGFIWDWVDQALLKEV NGRKRWAYGGEFGD+PNDS FC+NGVTWPDR
Sbjct: 601  YWEAIDNTFGLQGGFIWDWVDQALLKEVGNGRKRWAYGGEFGDIPNDSVFCMNGVTWPDR 660

Query: 685  TPHPALHEVKYLYQPIKISSKDGALQVLNAHFFSTTEDLEFSWSIYGDGLELGNGILSLP 744
            TPHPAL+E+K LYQ IK S KDG L+VLNAHFF+TTEDLEFSWSIYGDGLELGNGILSLP
Sbjct: 661  TPHPALNEIKNLYQEIKFSFKDGTLKVLNAHFFTTTEDLEFSWSIYGDGLELGNGILSLP 720

Query: 745  VIGPQESYNIEWQTSPWYDLWASSPALEFFLNVSVKLLHSTRWVEAGHIISSSQVQLPMK 804
            V+GPQ ++NIEWQ+SPWYDLWASSPALEFFL VSVK LHSTRWV+AGH ISSSQVQLPMK
Sbjct: 721  VLGPQGTHNIEWQSSPWYDLWASSPALEFFLTVSVKRLHSTRWVDAGHTISSSQVQLPMK 780

Query: 805  REFFPHSIKNGSASLLSEILGDTVRVYQQNLWEIKLDVQTGTLESWKVKGVPLIIKGIVP 864
            RE+FPHSIKN +++LLSEILGDTVRVYQQ+LWEIKLDVQTGTLESWKVKGVPLIIKGI+P
Sbjct: 781  REYFPHSIKNENSTLLSEILGDTVRVYQQDLWEIKLDVQTGTLESWKVKGVPLIIKGIMP 840

Query: 865  SFWRAPTDNDKGGGSCSYLSLWKAAHIDSLSFTAERCSILSTTEHYVKIAVVFLGLENDE 924
            SFWRAPTDNDKGGGSCSY SLWKAAHIDSLSF+AERCSILSTTEHYVK+A+VFLG+ +D+
Sbjct: 841  SFWRAPTDNDKGGGSCSYFSLWKAAHIDSLSFSAERCSILSTTEHYVKVAIVFLGVGSDD 900

Query: 925  QQASFSDSEKSNVLIQVDMTYTIYGSGDVIVDCNVQPSPNLPPLPRVGVEFHLDKSVDQV 984
            QQ S SDSEKSN+LIQVDMTYTI+GSGDVIVDC VQPSPNLPPLPRVGV+F+LDKS+D+V
Sbjct: 901  QQTSSSDSEKSNILIQVDMTYTIFGSGDVIVDCKVQPSPNLPPLPRVGVKFNLDKSMDRV 960

Query: 985  RWYGRGPFECYPDRKAAAHVGVYEKNVSEMHVPYIVPGESSGRADVRWVTFENKDGVGIY 1044
            RWYGRGPFECYPDRKAAAHVGVYEKNVSEMHVPYIVPGESSGR DVRWVT ENKDG+GIY
Sbjct: 961  RWYGRGPFECYPDRKAAAHVGVYEKNVSEMHVPYIVPGESSGRTDVRWVTLENKDGIGIY 1020

Query: 1045 ASIYRGSPPMQMSASYYSTEELDRAVHNEDLVEGDDIEVHLDHKHMGVGGDDSWSPCVHD 1104
            ASIY  SPPMQMSASYYS+ ELDRA HNE+LVEGDDIEVHLDHKHMGVGGDDSWSPCVHD
Sbjct: 1021 ASIYGSSPPMQMSASYYSSAELDRAEHNEELVEGDDIEVHLDHKHMGVGGDDSWSPCVHD 1080

Query: 1105 EYLLSPVPYSFSIRFYPLTPSASGYDAYKSQLPL 1139
            EYLL PVPYSFSIRF P+TPS S YDAY+SQLP+
Sbjct: 1081 EYLLPPVPYSFSIRFCPVTPSTSAYDAYRSQLPV 1114

BLAST of Spg009799 vs. TAIR 10
Match: AT3G54440.1 (glycoside hydrolase family 2 protein )

HSP 1 Score: 1688.3 bits (4371), Expect = 0.0e+00
Identity = 751/1113 (67.48%), Postives = 915/1113 (82.21%), Query Frame = 0

Query: 25   MASLAAKLMIPSENGYRVWEDQNFIKWKKRDSHVPLRCQDSVEGCLKYWHDRSKVDFLVS 84
            M SLA ++++PSENGYRVWEDQ   KW+KRD HV LRC +SV+G L+YW+ R+ VD  VS
Sbjct: 1    MVSLATQMILPSENGYRVWEDQTLFKWRKRDPHVTLRCHESVQGALRYWYQRNNVDLTVS 60

Query: 85   NSAVWNDDAVQSALDCAAFWVKGLPFIKSLSGYWKFYLAATPTSVPENFHGSAFEDSEWT 144
             SAVWNDDAVQ+ALD AAFWV GLPF+KSLSGYWKF+LA  P +VP+ F+ +AF DS+W 
Sbjct: 61   KSAVWNDDAVQAALDSAAFWVDGLPFVKSLSGYWKFFLAPKPANVPDKFYDAAFSDSDWN 120

Query: 145  NLPVPSNWQMHGFDRPIYTNVVYPFPLDPPHVPEDNPTGCYRTYFQLPEEWKGRRILLHF 204
             L VPSNWQ HGFDRPIYTNVVYPFP DPP+VPEDNPTGCYRTYFQ+P+EWK RRILLHF
Sbjct: 121  ALQVPSNWQCHGFDRPIYTNVVYPFPNDPPYVPEDNPTGCYRTYFQIPKEWKDRRILLHF 180

Query: 205  EAVDCAFFVWINGSLVGYSQDSRLPAEFEITEYCHPCGSKSKNVLAVQVLKWSDGSYLED 264
            EAVD AFF WING+ VGYSQDSRLPAEFEI++YC+P  S  +NVLAVQV +WSDGSYLED
Sbjct: 181  EAVDSAFFAWINGNPVGYSQDSRLPAEFEISDYCYPWDSGKQNVLAVQVFRWSDGSYLED 240

Query: 265  QDQWWLSGIHRDVLLLSKPQVFIGDYFFKSCVGEDFSYADIQVEVKIDNSLDTSKENFLN 324
            QD WWLSGIHRDVLLL+KP+VFI DYFFKS + +DFSYADIQVEVKIDN  ++SK+  L+
Sbjct: 241  QDHWWLSGIHRDVLLLAKPKVFIADYFFKSKLADDFSYADIQVEVKIDNMQESSKDLVLS 300

Query: 325  DFKLEAILFDNRTWDNHEGQADLLSSNVANVKLTLLSATTLGFHGYVLGGRLEKPKLWSA 384
            +F +EA +FD + W N EG +  LS  VAN+KL    + TLGFHGY+L G+L+ P LWSA
Sbjct: 301  NFIIEAAIFDTKNWYNSEGFSCELSPKVANLKLNPSPSPTLGFHGYLLEGKLDSPNLWSA 360

Query: 385  EHPQLYTLIILLKDLSDQIVDCESCLVGIRSITKAPKQLLVNGCPVVIRGVNRHEHHPRL 444
            E P +Y L++ LKD S +++D ES +VGIR ++KA KQLLVNG PVVI+GVNRHEHHPR+
Sbjct: 361  EQPNVYILVLTLKDTSGKVLDSESSIVGIRQVSKAFKQLLVNGHPVVIKGVNRHEHHPRV 420

Query: 445  GKTNIESCMVQDLVLMKQNNMNAVRNSHYPQHPRWYELCDLFGMYMIDEANIETHGFDYT 504
            GKTNIE+CMV+DL++MK+ N+NAVRNSHYPQHPRWYELCDLFGMYMIDEANIETHGFD +
Sbjct: 421  GKTNIEACMVKDLIMMKEYNINAVRNSHYPQHPRWYELCDLFGMYMIDEANIETHGFDLS 480

Query: 505  GHVKHPTLQPSWAAAMLDRVIGMVERDKNHACIIAWSLGNEAGYGPNHSALAGWIRGKDS 564
            GH+KHP  +PSWAAAMLDRV+GMVERDKNH CII+WSLGNEAGYGPNHSA+AGWIR KD 
Sbjct: 481  GHLKHPAKEPSWAAAMLDRVVGMVERDKNHTCIISWSLGNEAGYGPNHSAMAGWIREKDP 540

Query: 565  SRVLHYEGGGSRTSSTDIICPMYMRVWDMVKIANDPNETRPLILCEYSHSMGNSTGNLHK 624
            SR++HYEGGGSRTSSTDI+CPMYMRVWD++KIA D NE+RPLILCEY H+MGNS GN+ +
Sbjct: 541  SRLVHYEGGGSRTSSTDIVCPMYMRVWDIIKIALDQNESRPLILCEYQHAMGNSNGNIDE 600

Query: 625  YWEAIDNTFGLQGGFIWDWVDQALLKEVDNGRKRWAYGGEFGDVPNDSNFCLNGVTWPDR 684
            YWEAIDNTFGLQGGFIWDWVDQ LLK   +G KRWAYGG+FGD PND NFCLNG+ WPDR
Sbjct: 601  YWEAIDNTFGLQGGFIWDWVDQGLLKLGSDGIKRWAYGGDFGDQPNDLNFCLNGLIWPDR 660

Query: 685  TPHPALHEVKYLYQPIKISSKDGALQVLNAHFFSTTEDLEFSWSIYGDGLELGNGILSLP 744
            TPHPALHEVK+ YQPIK+S  DG ++V N +FF+TTE+LEFSW+I+GDGLELG+G LS+P
Sbjct: 661  TPHPALHEVKHCYQPIKVSLTDGMIKVANTYFFNTTEELEFSWTIHGDGLELGSGTLSIP 720

Query: 745  VIGPQESYNIEWQTSPWYDLWASSPALEFFLNVSVKLLHSTRWVEAGHIISSSQVQLPMK 804
            VI PQ S+ +EW++ PW+  W  S A E FL ++ KLL+ TR +EAGH++SS+Q+ LP K
Sbjct: 721  VIKPQNSFEMEWKSGPWFSFWNDSNAGELFLTINAKLLNLTRSLEAGHLLSSTQIPLPAK 780

Query: 805  REFFPHSIKNGSASLLSEILGDTVRVYQQNLWEIKLDVQTGTLESWKVKGVPLIIKGIVP 864
             +  P +IK    S+  E +GD +++ Q++ WE+ ++V+ GT+E WK++GV L+ + I+P
Sbjct: 781  GQIIPQAIKKTDTSITCETVGDFIKISQKDSWELMVNVRKGTIEGWKIQGVLLMNEAILP 840

Query: 865  SFWRAPTDNDKGGGSCSYLSLWKAAHIDSLSFTAERCSILSTTEHYVKIAVVFLGLENDE 924
             FWRAPTDNDKGGG  SY S WKAA +D++ F  E CS+ S T+  V+I  ++LG     
Sbjct: 841  CFWRAPTDNDKGGGDSSYFSRWKAAQLDNVEFLVESCSVKSITDKSVEIEFIYLG----- 900

Query: 925  QQASFSDSEKSNVLIQVDMTYTIYGSGDVIVDCNVQPSPNLPPLPRVGVEFHLDKSVDQV 984
              +S S S KS+ L +V++TY IYGSGD+I +  V+P+ +LPPLPRVG+EFH++K++D+V
Sbjct: 901  --SSASGSSKSDALFKVNVTYLIYGSGDIITNWFVEPNSDLPPLPRVGIEFHIEKTLDRV 960

Query: 985  RWYGRGPFECYPDRKAAAHVGVYEKNVSEMHVPYIVPGESSGRADVRWVTFENKDGVGIY 1044
             WYG+GPFECYPDRKAAAHV +YE NV +MHVPYIVPGE+ GR DVRWVTF NKDGVGIY
Sbjct: 961  EWYGKGPFECYPDRKAAAHVAIYEHNVGDMHVPYIVPGENGGRTDVRWVTFRNKDGVGIY 1020

Query: 1045 ASIYRGSPPMQMSASYYSTEELDRAVHNEDLVEGDDIEVHLDHKHMGVGGDDSWSPCVHD 1104
            AS Y  S  MQM+ASYY+T EL RA H EDL++G +IEVHLDHKHMG+GGDDSW+PCVHD
Sbjct: 1021 ASTYGSSSLMQMNASYYTTGELHRATHEEDLIKGQNIEVHLDHKHMGLGGDDSWTPCVHD 1080

Query: 1105 EYLLSPVPYSFSIRFYPLTPSASGYDAYKSQLP 1138
            ++L+ P  YSFS+R  P+T S SG + YK QLP
Sbjct: 1081 KFLIPPAQYSFSLRLCPITASTSGLNIYKDQLP 1106

BLAST of Spg009799 vs. TAIR 10
Match: AT3G54440.2 (glycoside hydrolase family 2 protein )

HSP 1 Score: 1683.7 bits (4359), Expect = 0.0e+00
Identity = 751/1114 (67.41%), Postives = 915/1114 (82.14%), Query Frame = 0

Query: 25   MASLAAKLMIPSENGYRVWEDQNFIKWKKRDSHVPLRCQDSVEGCLKYWHDRSKVDFLVS 84
            M SLA ++++PSENGYRVWEDQ   KW+KRD HV LRC +SV+G L+YW+ R+ VD  VS
Sbjct: 1    MVSLATQMILPSENGYRVWEDQTLFKWRKRDPHVTLRCHESVQGALRYWYQRNNVDLTVS 60

Query: 85   NSAVWNDDAVQSALDCAAFWVKGLPFIKSLSGYWKFYLAATPTSVPENFHGSAFEDSEWT 144
             SAVWNDDAVQ+ALD AAFWV GLPF+KSLSGYWKF+LA  P +VP+ F+ +AF DS+W 
Sbjct: 61   KSAVWNDDAVQAALDSAAFWVDGLPFVKSLSGYWKFFLAPKPANVPDKFYDAAFSDSDWN 120

Query: 145  NLPVPSNWQMHGFDRPIYTNVVYPFPLDPPHVPEDNPTGCYRTYFQLPEEWKGRRILLHF 204
             L VPSNWQ HGFDRPIYTNVVYPFP DPP+VPEDNPTGCYRTYFQ+P+EWK RRILLHF
Sbjct: 121  ALQVPSNWQCHGFDRPIYTNVVYPFPNDPPYVPEDNPTGCYRTYFQIPKEWKDRRILLHF 180

Query: 205  EAVDCAFFVWINGSLVGYSQDSRLPAEFEITEYCHPCGSKSKNVLAVQVLKWSDGSYLED 264
            EAVD AFF WING+ VGYSQDSRLPAEFEI++YC+P  S  +NVLAVQV +WSDGSYLED
Sbjct: 181  EAVDSAFFAWINGNPVGYSQDSRLPAEFEISDYCYPWDSGKQNVLAVQVFRWSDGSYLED 240

Query: 265  QDQWWLSGIHRDVLLLSKPQVFIGDYFFKSCVGEDFSYADIQVEVKIDNSLDTSKENFLN 324
            QD WWLSGIHRDVLLL+KP+VFI DYFFKS + +DFSYADIQVEVKIDN  ++SK+  L+
Sbjct: 241  QDHWWLSGIHRDVLLLAKPKVFIADYFFKSKLADDFSYADIQVEVKIDNMQESSKDLVLS 300

Query: 325  DFKLEAILFDNRTWDNHEGQADLLSSNVANVKLTLLSATTLGFHGYVLGGRLEKPKLWSA 384
            +F +EA +FD + W N EG +  LS  VAN+KL    + TLGFHGY+L G+L+ P LWSA
Sbjct: 301  NFIIEAAIFDTKNWYNSEGFSCELSPKVANLKLNPSPSPTLGFHGYLLEGKLDSPNLWSA 360

Query: 385  EHPQLYTLIILLKDLSDQIVDCESCLVGIRSITKAPKQLLVNGCPVVIRGVNRHEHHPRL 444
            E P +Y L++ LKD S +++D ES +VGIR ++KA KQLLVNG PVVI+GVNRHEHHPR+
Sbjct: 361  EQPNVYILVLTLKDTSGKVLDSESSIVGIRQVSKAFKQLLVNGHPVVIKGVNRHEHHPRV 420

Query: 445  GKTNIESCMVQDLVLMKQNNMNAVRNSHYPQHPRWYELCDLFGMYMIDEANIETHGFDYT 504
            GKTNIE+CMV+DL++MK+ N+NAVRNSHYPQHPRWYELCDLFGMYMIDEANIETHGFD +
Sbjct: 421  GKTNIEACMVKDLIMMKEYNINAVRNSHYPQHPRWYELCDLFGMYMIDEANIETHGFDLS 480

Query: 505  GHVKHPTLQPSWAAAMLDRVIGMVERDKNHACIIAWSLGNEAGYGPNHSALAGWIRGKDS 564
            GH+KHP  +PSWAAAMLDRV+GMVERDKNH CII+WSLGNEAGYGPNHSA+AGWIR KD 
Sbjct: 481  GHLKHPAKEPSWAAAMLDRVVGMVERDKNHTCIISWSLGNEAGYGPNHSAMAGWIREKDP 540

Query: 565  SRVLHYEGGGSRTSSTDIICPMYMRVWDMVKIANDPNETRPLILCEYSHSMGNSTGNLHK 624
            SR++HYEGGGSRTSSTDI+CPMYMRVWD++KIA D NE+RPLILCEY H+MGNS GN+ +
Sbjct: 541  SRLVHYEGGGSRTSSTDIVCPMYMRVWDIIKIALDQNESRPLILCEYQHAMGNSNGNIDE 600

Query: 625  YWEAIDNTFGLQGGFIWDWVDQALLKEVDNGRKRWAYGGEFGDVPNDSNFCLNGVTWPDR 684
            YWEAIDNTFGLQGGFIWDWVDQ LLK   +G KRWAYGG+FGD PND NFCLNG+ WPDR
Sbjct: 601  YWEAIDNTFGLQGGFIWDWVDQGLLKLGSDGIKRWAYGGDFGDQPNDLNFCLNGLIWPDR 660

Query: 685  TPHPALHEVKYLYQPIKISSKDGALQVLNAHFFSTTEDLEFSWSIYGDGLELGNGILSLP 744
            TPHPALHEVK+ YQPIK+S  DG ++V N +FF+TTE+LEFSW+I+GDGLELG+G LS+P
Sbjct: 661  TPHPALHEVKHCYQPIKVSLTDGMIKVANTYFFNTTEELEFSWTIHGDGLELGSGTLSIP 720

Query: 745  VIGPQESYNIEWQTSPWYDLWASSPALEFFLNVSVKLLHSTRWVEAGHIISSSQVQLPMK 804
            VI PQ S+ +EW++ PW+  W  S A E FL ++ KLL+ TR +EAGH++SS+Q+ LP K
Sbjct: 721  VIKPQNSFEMEWKSGPWFSFWNDSNAGELFLTINAKLLNLTRSLEAGHLLSSTQIPLPAK 780

Query: 805  REFFPHSIKNGSASLLSEILGDTVRVYQQNLWEIKLDVQTGTLESWKVKGVPLIIKGIVP 864
             +  P +IK    S+  E +GD +++ Q++ WE+ ++V+ GT+E WK++GV L+ + I+P
Sbjct: 781  GQIIPQAIKKTDTSITCETVGDFIKISQKDSWELMVNVRKGTIEGWKIQGVLLMNEAILP 840

Query: 865  SFWRAPTDNDKGGGSCSYLSLWKAAHIDSLSFTAERCSILSTTEHYVKIAVVFLGLENDE 924
             FWRAPTDNDKGGG  SY S WKAA +D++ F  E CS+ S T+  V+I  ++LG     
Sbjct: 841  CFWRAPTDNDKGGGDSSYFSRWKAAQLDNVEFLVESCSVKSITDKSVEIEFIYLG----- 900

Query: 925  QQASFSDSEKSNVLIQVDMTYTIYGSGDVIVDCNVQPSPNLPPLPRVGVEFHLDKSVDQV 984
              +S S S KS+ L +V++TY IYGSGD+I +  V+P+ +LPPLPRVG+EFH++K++D+V
Sbjct: 901  --SSASGSSKSDALFKVNVTYLIYGSGDIITNWFVEPNSDLPPLPRVGIEFHIEKTLDRV 960

Query: 985  RWYGRGPFECYPDRKAAAHVGVYEKNVSEMHVPYIVPGESSGRADVRWVTFENKDGVGIY 1044
             WYG+GPFECYPDRKAAAHV +YE NV +MHVPYIVPGE+ GR DVRWVTF NKDGVGIY
Sbjct: 961  EWYGKGPFECYPDRKAAAHVAIYEHNVGDMHVPYIVPGENGGRTDVRWVTFRNKDGVGIY 1020

Query: 1045 ASIYRGSPPMQMSASYYSTEELDRAVHNEDLVEGDDIE-VHLDHKHMGVGGDDSWSPCVH 1104
            AS Y  S  MQM+ASYY+T EL RA H EDL++G +IE VHLDHKHMG+GGDDSW+PCVH
Sbjct: 1021 ASTYGSSSLMQMNASYYTTGELHRATHEEDLIKGQNIEVVHLDHKHMGLGGDDSWTPCVH 1080

Query: 1105 DEYLLSPVPYSFSIRFYPLTPSASGYDAYKSQLP 1138
            D++L+ P  YSFS+R  P+T S SG + YK QLP
Sbjct: 1081 DKFLIPPAQYSFSLRLCPITASTSGLNIYKDQLP 1107

BLAST of Spg009799 vs. TAIR 10
Match: AT3G54440.3 (glycoside hydrolase family 2 protein )

HSP 1 Score: 1679.1 bits (4347), Expect = 0.0e+00
Identity = 751/1126 (66.70%), Postives = 915/1126 (81.26%), Query Frame = 0

Query: 25   MASLAAKLMIPSENGYRVWEDQNFIKWKKRDSHVPLRCQDSVE-------------GCLK 84
            M SLA ++++PSENGYRVWEDQ   KW+KRD HV LRC +SV+             G L+
Sbjct: 1    MVSLATQMILPSENGYRVWEDQTLFKWRKRDPHVTLRCHESVQVSQGRVKILCDCIGALR 60

Query: 85   YWHDRSKVDFLVSNSAVWNDDAVQSALDCAAFWVKGLPFIKSLSGYWKFYLAATPTSVPE 144
            YW+ R+ VD  VS SAVWNDDAVQ+ALD AAFWV GLPF+KSLSGYWKF+LA  P +VP+
Sbjct: 61   YWYQRNNVDLTVSKSAVWNDDAVQAALDSAAFWVDGLPFVKSLSGYWKFFLAPKPANVPD 120

Query: 145  NFHGSAFEDSEWTNLPVPSNWQMHGFDRPIYTNVVYPFPLDPPHVPEDNPTGCYRTYFQL 204
             F+ +AF DS+W  L VPSNWQ HGFDRPIYTNVVYPFP DPP+VPEDNPTGCYRTYFQ+
Sbjct: 121  KFYDAAFSDSDWNALQVPSNWQCHGFDRPIYTNVVYPFPNDPPYVPEDNPTGCYRTYFQI 180

Query: 205  PEEWKGRRILLHFEAVDCAFFVWINGSLVGYSQDSRLPAEFEITEYCHPCGSKSKNVLAV 264
            P+EWK RRILLHFEAVD AFF WING+ VGYSQDSRLPAEFEI++YC+P  S  +NVLAV
Sbjct: 181  PKEWKDRRILLHFEAVDSAFFAWINGNPVGYSQDSRLPAEFEISDYCYPWDSGKQNVLAV 240

Query: 265  QVLKWSDGSYLEDQDQWWLSGIHRDVLLLSKPQVFIGDYFFKSCVGEDFSYADIQVEVKI 324
            QV +WSDGSYLEDQD WWLSGIHRDVLLL+KP+VFI DYFFKS + +DFSYADIQVEVKI
Sbjct: 241  QVFRWSDGSYLEDQDHWWLSGIHRDVLLLAKPKVFIADYFFKSKLADDFSYADIQVEVKI 300

Query: 325  DNSLDTSKENFLNDFKLEAILFDNRTWDNHEGQADLLSSNVANVKLTLLSATTLGFHGYV 384
            DN  ++SK+  L++F +EA +FD + W N EG +  LS  VAN+KL    + TLGFHGY+
Sbjct: 301  DNMQESSKDLVLSNFIIEAAIFDTKNWYNSEGFSCELSPKVANLKLNPSPSPTLGFHGYL 360

Query: 385  LGGRLEKPKLWSAEHPQLYTLIILLKDLSDQIVDCESCLVGIRSITKAPKQLLVNGCPVV 444
            L G+L+ P LWSAE P +Y L++ LKD S +++D ES +VGIR ++KA KQLLVNG PVV
Sbjct: 361  LEGKLDSPNLWSAEQPNVYILVLTLKDTSGKVLDSESSIVGIRQVSKAFKQLLVNGHPVV 420

Query: 445  IRGVNRHEHHPRLGKTNIESCMVQDLVLMKQNNMNAVRNSHYPQHPRWYELCDLFGMYMI 504
            I+GVNRHEHHPR+GKTNIE+CMV+DL++MK+ N+NAVRNSHYPQHPRWYELCDLFGMYMI
Sbjct: 421  IKGVNRHEHHPRVGKTNIEACMVKDLIMMKEYNINAVRNSHYPQHPRWYELCDLFGMYMI 480

Query: 505  DEANIETHGFDYTGHVKHPTLQPSWAAAMLDRVIGMVERDKNHACIIAWSLGNEAGYGPN 564
            DEANIETHGFD +GH+KHP  +PSWAAAMLDRV+GMVERDKNH CII+WSLGNEAGYGPN
Sbjct: 481  DEANIETHGFDLSGHLKHPAKEPSWAAAMLDRVVGMVERDKNHTCIISWSLGNEAGYGPN 540

Query: 565  HSALAGWIRGKDSSRVLHYEGGGSRTSSTDIICPMYMRVWDMVKIANDPNETRPLILCEY 624
            HSA+AGWIR KD SR++HYEGGGSRTSSTDI+CPMYMRVWD++KIA D NE+RPLILCEY
Sbjct: 541  HSAMAGWIREKDPSRLVHYEGGGSRTSSTDIVCPMYMRVWDIIKIALDQNESRPLILCEY 600

Query: 625  SHSMGNSTGNLHKYWEAIDNTFGLQGGFIWDWVDQALLKEVDNGRKRWAYGGEFGDVPND 684
             H+MGNS GN+ +YWEAIDNTFGLQGGFIWDWVDQ LLK   +G KRWAYGG+FGD PND
Sbjct: 601  QHAMGNSNGNIDEYWEAIDNTFGLQGGFIWDWVDQGLLKLGSDGIKRWAYGGDFGDQPND 660

Query: 685  SNFCLNGVTWPDRTPHPALHEVKYLYQPIKISSKDGALQVLNAHFFSTTEDLEFSWSIYG 744
             NFCLNG+ WPDRTPHPALHEVK+ YQPIK+S  DG ++V N +FF+TTE+LEFSW+I+G
Sbjct: 661  LNFCLNGLIWPDRTPHPALHEVKHCYQPIKVSLTDGMIKVANTYFFNTTEELEFSWTIHG 720

Query: 745  DGLELGNGILSLPVIGPQESYNIEWQTSPWYDLWASSPALEFFLNVSVKLLHSTRWVEAG 804
            DGLELG+G LS+PVI PQ S+ +EW++ PW+  W  S A E FL ++ KLL+ TR +EAG
Sbjct: 721  DGLELGSGTLSIPVIKPQNSFEMEWKSGPWFSFWNDSNAGELFLTINAKLLNLTRSLEAG 780

Query: 805  HIISSSQVQLPMKREFFPHSIKNGSASLLSEILGDTVRVYQQNLWEIKLDVQTGTLESWK 864
            H++SS+Q+ LP K +  P +IK    S+  E +GD +++ Q++ WE+ ++V+ GT+E WK
Sbjct: 781  HLLSSTQIPLPAKGQIIPQAIKKTDTSITCETVGDFIKISQKDSWELMVNVRKGTIEGWK 840

Query: 865  VKGVPLIIKGIVPSFWRAPTDNDKGGGSCSYLSLWKAAHIDSLSFTAERCSILSTTEHYV 924
            ++GV L+ + I+P FWRAPTDNDKGGG  SY S WKAA +D++ F  E CS+ S T+  V
Sbjct: 841  IQGVLLMNEAILPCFWRAPTDNDKGGGDSSYFSRWKAAQLDNVEFLVESCSVKSITDKSV 900

Query: 925  KIAVVFLGLENDEQQASFSDSEKSNVLIQVDMTYTIYGSGDVIVDCNVQPSPNLPPLPRV 984
            +I  ++LG       +S S S KS+ L +V++TY IYGSGD+I +  V+P+ +LPPLPRV
Sbjct: 901  EIEFIYLG-------SSASGSSKSDALFKVNVTYLIYGSGDIITNWFVEPNSDLPPLPRV 960

Query: 985  GVEFHLDKSVDQVRWYGRGPFECYPDRKAAAHVGVYEKNVSEMHVPYIVPGESSGRADVR 1044
            G+EFH++K++D+V WYG+GPFECYPDRKAAAHV +YE NV +MHVPYIVPGE+ GR DVR
Sbjct: 961  GIEFHIEKTLDRVEWYGKGPFECYPDRKAAAHVAIYEHNVGDMHVPYIVPGENGGRTDVR 1020

Query: 1045 WVTFENKDGVGIYASIYRGSPPMQMSASYYSTEELDRAVHNEDLVEGDDIEVHLDHKHMG 1104
            WVTF NKDGVGIYAS Y  S  MQM+ASYY+T EL RA H EDL++G +IEVHLDHKHMG
Sbjct: 1021 WVTFRNKDGVGIYASTYGSSSLMQMNASYYTTGELHRATHEEDLIKGQNIEVHLDHKHMG 1080

Query: 1105 VGGDDSWSPCVHDEYLLSPVPYSFSIRFYPLTPSASGYDAYKSQLP 1138
            +GGDDSW+PCVHD++L+ P  YSFS+R  P+T S SG + YK QLP
Sbjct: 1081 LGGDDSWTPCVHDKFLIPPAQYSFSLRLCPITASTSGLNIYKDQLP 1119

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038891496.10.0e+0092.01beta-galactosidase isoform X1 [Benincasa hispida][more]
XP_023526876.10.0e+0090.93uncharacterized protein LOC111790243 [Cucurbita pepo subsp. pepo][more]
KAG6601128.10.0e+0090.87hypothetical protein SDJN03_06361, partial [Cucurbita argyrosperma subsp. sorori... [more]
KAG7031926.10.0e+0090.93lacZ [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022956821.10.0e+0090.75uncharacterized protein LOC111458406 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A5F5U66.2e-22439.84Beta-galactosidase OS=Vibrio cholerae serotype O1 (strain ATCC 39541 / Classical... [more]
Q6LL683.2e-22040.39Beta-galactosidase OS=Photobacterium profundum (strain SS9) OX=298386 GN=lacZ PE... [more]
Q8D4H34.6e-21939.73Beta-galactosidase OS=Vibrio vulnificus (strain CMCP6) OX=216895 GN=lacZ PE=3 SV... [more]
P816501.3e-21838.92Beta-galactosidase OS=Pseudoalteromonas haloplanktis OX=228 GN=lacZ PE=1 SV=2[more]
Q7MG042.3e-21839.71Beta-galactosidase OS=Vibrio vulnificus (strain YJ016) OX=196600 GN=lacZ PE=3 SV... [more]
Match NameE-valueIdentityDescription
A0A6J1GXK40.0e+0090.75Lactase OS=Cucurbita moschata OX=3662 GN=LOC111458406 PE=3 SV=1[more]
A0A5D3CBT70.0e+0090.31Lactase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G003100 PE=3 ... [more]
A0A1S3BGB30.0e+0090.31Lactase OS=Cucumis melo OX=3656 GN=LOC103489515 PE=3 SV=1[more]
A0A6J1CDH40.0e+0090.14Lactase OS=Momordica charantia OX=3673 GN=LOC111010223 PE=3 SV=1[more]
A0A6J1JEA60.0e+0090.39Lactase OS=Cucurbita maxima OX=3661 GN=LOC111486104 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G54440.10.0e+0067.48glycoside hydrolase family 2 protein [more]
AT3G54440.20.0e+0067.41glycoside hydrolase family 2 protein [more]
AT3G54440.30.0e+0066.70glycoside hydrolase family 2 protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006101Glycoside hydrolase, family 2PRINTSPR00132GLHYDRLASE2coord: 425..439
score: 59.54
coord: 531..546
score: 65.52
coord: 207..222
score: 47.41
coord: 604..619
score: 62.5
coord: 456..474
score: 64.79
IPR004199Beta galactosidase small chain/ domain 5SMARTSM01038Bgal_small_N_2coord: 834..1120
e-value: 1.0E-110
score: 383.8
IPR004199Beta galactosidase small chain/ domain 5PFAMPF02929Bgal_small_Ncoord: 835..1118
e-value: 2.1E-76
score: 256.8
IPR006103Glycoside hydrolase family 2, catalytic domainPFAMPF02836Glyco_hydro_2_Ccoord: 421..702
e-value: 8.1E-95
score: 317.4
IPR014718Glycoside hydrolase-type carbohydrate-bindingGENE3D2.70.98.10coord: 823..1120
e-value: 3.1E-95
score: 320.7
NoneNo IPR availableGENE3D2.60.120.260coord: 32..283
e-value: 5.6E-76
score: 256.9
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 416..696
e-value: 2.4E-105
score: 354.4
NoneNo IPR availablePANTHERPTHR46323:SF2GLYCOSIDE HYDROLASE FAMILY 2 PROTEINcoord: 31..1125
IPR006102Glycoside hydrolase, family 2, immunoglobulin-like beta-sandwichPFAMPF00703Glyco_hydro_2coord: 285..414
e-value: 9.2E-10
score: 39.2
IPR013783Immunoglobulin-like foldGENE3D2.60.40.10Immunoglobulinscoord: 284..414
e-value: 4.5E-18
score: 68.3
IPR013783Immunoglobulin-like foldGENE3D2.60.40.10Immunoglobulinscoord: 698..806
e-value: 4.3E-20
score: 73.5
IPR032312Beta-galactosidase, domain 4PFAMPF16353DUF4981coord: 710..802
e-value: 1.6E-16
score: 60.5
IPR006104Glycosyl hydrolases family 2, sugar binding domainPFAMPF02837Glyco_hydro_2_Ncoord: 111..283
e-value: 1.5E-48
score: 164.9
IPR023933Glycoside hydrolase, family 2, beta-galactosidasePANTHERPTHR46323BETA-GALACTOSIDASEcoord: 31..1125
IPR023230Glycoside hydrolase, family 2, conserved sitePROSITEPS00719GLYCOSYL_HYDROL_F2_1coord: 466..491
IPR008979Galactose-binding-like domain superfamilySUPERFAMILY49785Galactose-binding domain-likecoord: 110..283
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 419..698
IPR011013Galactose mutarotase-like domain superfamilySUPERFAMILY74650Galactose mutarotase-likecoord: 823..1121
IPR036156Beta-Galactosidase/glucuronidase domain superfamilySUPERFAMILY49303beta-Galactosidase/glucuronidase domaincoord: 285..414
IPR036156Beta-Galactosidase/glucuronidase domain superfamilySUPERFAMILY49303beta-Galactosidase/glucuronidase domaincoord: 694..803

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg009799.1Spg009799.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005990 lactose catabolic process
biological_process GO:0005975 carbohydrate metabolic process
cellular_component GO:0009341 beta-galactosidase complex
molecular_function GO:0004565 beta-galactosidase activity
molecular_function GO:0030246 carbohydrate binding
molecular_function GO:0003824 catalytic activity
molecular_function GO:0016798 hydrolase activity, acting on glycosyl bonds
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds