Spg008806 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg008806
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionNitrate reductase
Locationscaffold10: 34451068 .. 34454650 (+)
RNA-Seq ExpressionSpg008806
SyntenySpg008806
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CGCCTATATAATCCCACCGTCTCCGCTTTACTTTTTCCCACCTTCACAACTTCATTCAAATCAAACTTTACAAAGAAAGTGAAGTGAAGGAAGCTGGTGGCCCAAAAGAAAACAACCATTTCTCTTTTCCCACCCAGAGCAACCGATCTCCCATGGCGGCCTCCGTCGACAACCGCCAGTTCGGCCCTCTTCAGCCACCGCTCAACGGCGTCGTCAGGACCTTTAAGTCCGGTCCCAACCACCGCTCCGACTCTCCCGTCCGTGGCTGTAATTTCCCCAATTCCAACATCGACTACAGCAACGGCAACCGTTCCGTGAAAAGTTCTGTGAAGATAGAAGAGGAGGAGGATTCCAGTAGTGACGATGAGAATGAGATCAACGAGTTTCGTGAGTTGATCAAGAAGGGAAACCGTGAACTGGAGCCGTCGATTGTGGACCCCAGAGATGAAGGAACGGCTGATAATTGGATCGAGCGGAATTCTTCCATGGTCCGTCTCACCGGGAAGCACCCCTTCAACTCCGAGCCGCCTCTCAACCGTCTCATGCACCACGGCTTCATCACCCCGGTCCCACTCCACTACGTGCGCAACCACGGCGCGGTCCCCAAGGCCAAGTGGAGCGACTGGAGCATCGAGATCTGCGGGCTCGTCAAGCGCCCCGCCAAGTTCACCATGGACCAACTCGTCAACGAGTTTCGCTTCCGGGAGTTTCCGGCAACACTCGTCTGCGCCGGCAACCGGCGGAAGGAGCAGAACATGGTGAAAAAGACCATTGGGTTCAACTGGGGAGCGGCGGGGGTGTCCACGTCGGTCTGGCGAGGGGTCCCACTGCGTGACGTGTTGAAACGATGCGGGATTTTTAGCCGGAAGAAGGGCGCGCTGAATGTGTGCTTTGAAGGAGCGGAGGACTTGCCAGGTGGCGGTGGGTCCAAGTACGGGACCAGCATCAAGAAGGAGCTGGCTATGGATCCGGCTAGGGACATCATTTTGGCTTACATGCAGAACGGCGAACAGTTGTCGCCGGATCATGGATTTCCCGTGAGGATGATAATACCGGGATTCATTGGGGGTAGAATGGTAAAGTGGCTCAAACGGATCATTGTGACAACCAAAGAATCCGAAAATTATTACCATTACAAGGATAACAGAGTATTACCTTCACATGTCGACGCTGAGCTGGCCAACTCCGAAGGTAATTTACAAATTGGTTAATTCTAATTGGTTGGCTGTATGAAAACGTAGCTAGATTAATTGAAGTTTTTAATTAATATGCAGCATGGTGGTACAAGCCTGAGTATATAATCAACGAGTTGAACATTAACTCCGTGATAACGACGCCCTGCCACGAGGAGATTTTACCCATCAACTCCTGGACCACACAGAGGCCGTACACCTTGAGAGGCTACTCTTATTCCGGTGAGCCGCCGCTGTTTTATTTAATTTTTTATTTTGACTCCCCCAGGCAAGGATATTTTTTTTTATTTAATAATTCTTTTAACTAGAGAATCTCTCGTTTTTGGAATAAATAAGTTGATTTATTTTAATAAACAAATGGTTTGGTTTACTTTAAGATACCTTTTCTTTAGTGTTATTTAGGAAAGTAGATGTAGATCCGCATCCGCTGAGTTTTAGTCAAATAATTTTAATTTTTTTGAATTAGATGTATTTTTAATTTTATATATGTTTGAAAAACGAGTAGGTGGTGGGAAGAAGGTCACACGTGTGGAGGTTACAATGGACAGTGGAGAGACATGGCACGTGTGCACGTTGGACCATCCGGAGAAGCCAAACAAGTTTGGAAAATACTGGTGCTGGTGTTTCTGGTCGTTGGAAGTGGAGGTTCTGGACTTGCTTGGCGCCAAGGAAATCGCGGTTCGGGCTTGGGACGAAACCCTCAATACCCAACCCGAGAAACTCATCTGGAACCTCATGGTATGGTACATAAAATATAAAACAAACTCATCCCATTTAACATTAATTTCTAGTCGCTGATCCAATGTAAACATTAAATTATTAATTTACAGGGAATGATGAACAACTGTTGGTTCAGAGTGAAAACGAACGTATGCAAACCCCACAAGGGAGAGATCGGAATCATCTTCGAACACCCGACACTCCCGGGCAACCAGTCCGGCGGATGGATGGACAGAGAGAGACATCTGGAAATATCCTCCGAGTCCAACCAAACCCTGAAGAAGAGCGTCTCCACTCCATTCATGAACACCGCCTCCAACACCTACTCCTTATCGGAAGTCAAAAAGCACAACTCCCCTCAGTCCGCCTGGATCATCGTCCACGGCCACGTCTACGACTGCACCCGCTTCCTCAAAGACCACCCCGGCGGCTCCGACAGCATTCTGATCAACGCCGGCACCGACTGCACCGAAGAGTTCGACGCCATCCACTCCGACAAAGCCAAGAAAATGCTCGAAGACTACCGAATCGGCGAGTTGATCACCACCGGCTACGCCTCCGACTCCTCCTCCAACTCCCCCAACAACTCCACTCACGGCGCTTCCAATTTCTCCCACTTAGCCCCCATCAAGGAAATCACTCCTTCCTCGCCAATCCCAGCACGACGCGTGGCACTCGCCCCCAACCAGAAAATCCCCTGCAAGCTCGTGTCGAAGACCGCAATCTCACACGACGTGCGTGTCTTCAGATTCGCGCTGCCCGGTCAGGAGCAGACGCTGGGGCTGCCCGTGGGCAAGCACATTTTCATATGCGCCACGGTGGACGGCAAGCTCTGCATGAGGGCCTACACGCCCACGAGCTCAATAGACGAAGTGGGCTTCTTTGAATTGGTCGTCAAAGTCTACTTCAAGAACGTTCATCCGAAATTTCCCAACGGTGGAGTGATGTCGCAGTACTTGGAGTCGATGGAGGTGGGTTCGACGCTGGACGTGAAGGGTCCGCTGGGGCACATCGAGTACACGGGGCGGGGAAACTTCATGGTCCATGGGAAGCCCCGGTTCGCGAAGCGGCTGGCGATGCTGGCGGGTGGGACCGGGATAACGCCGATTTATCAAGTGGTGCAGGCGATATTGAAGGATCCGGAGGACGAGACGGAGATGTTTGTGGTGTATGCGAATCGGACGGAGGAGGATATTTTGCTGAGGGAGGAATTGGAGATGTGGGCCAAGAAGAATGACAGATTGAAAGTGTGGTACGTGGTGCAGGAGAGTATTAGAGAAGGCTGGGAATACAGCGTTGGGTTTATCACGGAGAATATTCTGAGAGAGCATATTCCGACGGCGGCGGAGGATACTTTGGCTCTCGTATGTGGGCCGCCGCCGATGATTCAGTTCGCGGTGCAACCCAATTTGGAGAAGATGAATTACGACACCAAGAATTCGATGCTGGTGTTTTAATTAGACAGAGTACATAAATAAATAAAATTGCTTTTTTTTTATTTTTTTTCTTCAATGGAATGGGGAATTGAAGAAAAGGACCGTGGATTCGTTTTGTAAAATAATGAAATATATGTGTATATTAAGTTGTAAATATATATATATTTTTTTCCTTCTTCTTCTTCTTTGATGTATTCATGCATAAGGGACGATAATAAACGTTGTCGTTTATTC

mRNA sequence

ATGGCGGCCTCCGTCGACAACCGCCAGTTCGGCCCTCTTCAGCCACCGCTCAACGGCGTCGTCAGGACCTTTAAGTCCGGTCCCAACCACCGCTCCGACTCTCCCGTCCGTGGCTGTAATTTCCCCAATTCCAACATCGACTACAGCAACGGCAACCGTTCCGTGAAAAGTTCTGTGAAGATAGAAGAGGAGGAGGATTCCAGTAGTGACGATGAGAATGAGATCAACGAGTTTCGTGAGTTGATCAAGAAGGGAAACCGTGAACTGGAGCCGTCGATTGTGGACCCCAGAGATGAAGGAACGGCTGATAATTGGATCGAGCGGAATTCTTCCATGGTCCGTCTCACCGGGAAGCACCCCTTCAACTCCGAGCCGCCTCTCAACCGTCTCATGCACCACGGCTTCATCACCCCGGTCCCACTCCACTACGTGCGCAACCACGGCGCGGTCCCCAAGGCCAAGTGGAGCGACTGGAGCATCGAGATCTGCGGGCTCGTCAAGCGCCCCGCCAAGTTCACCATGGACCAACTCGTCAACGAGTTTCGCTTCCGGGAGTTTCCGGCAACACTCGTCTGCGCCGGCAACCGGCGGAAGGAGCAGAACATGGTGAAAAAGACCATTGGGTTCAACTGGGGAGCGGCGGGGGTGTCCACGTCGGTCTGGCGAGGGGTCCCACTGCGTGACGTGTTGAAACGATGCGGGATTTTTAGCCGGAAGAAGGGCGCGCTGAATGTGTGCTTTGAAGGAGCGGAGGACTTGCCAGGTGGCGGTGGGTCCAAGTACGGGACCAGCATCAAGAAGGAGCTGGCTATGGATCCGGCTAGGGACATCATTTTGGCTTACATGCAGAACGGCGAACAGTTGTCGCCGGATCATGGATTTCCCGTGAGGATGATAATACCGGGATTCATTGGGGGTAGAATGGTAAAGTGGCTCAAACGGATCATTGTGACAACCAAAGAATCCGAAAATTATTACCATTACAAGGATAACAGAGTATTACCTTCACATGTCGACGCTGAGCTGGCCAACTCCGAAGCATGGTGGTACAAGCCTGAGTATATAATCAACGAGTTGAACATTAACTCCGTGATAACGACGCCCTGCCACGAGGAGATTTTACCCATCAACTCCTGGACCACACAGAGGCCGTACACCTTGAGAGGCTACTCTTATTCCGGTGGTGGGAAGAAGGTCACACGTGTGGAGGTTACAATGGACAGTGGAGAGACATGGCACGTGTGCACGTTGGACCATCCGGAGAAGCCAAACAAGTTTGGAAAATACTGGTGCTGGTGTTTCTGGTCGTTGGAAGTGGAGGTTCTGGACTTGCTTGGCGCCAAGGAAATCGCGGTTCGGGCTTGGGACGAAACCCTCAATACCCAACCCGAGAAACTCATCTGGAACCTCATGGGAATGATGAACAACTGTTGGTTCAGAGTGAAAACGAACGTATGCAAACCCCACAAGGGAGAGATCGGAATCATCTTCGAACACCCGACACTCCCGGGCAACCAGTCCGGCGGATGGATGGACAGAGAGAGACATCTGGAAATATCCTCCGAGTCCAACCAAACCCTGAAGAAGAGCGTCTCCACTCCATTCATGAACACCGCCTCCAACACCTACTCCTTATCGGAAGTCAAAAAGCACAACTCCCCTCAGTCCGCCTGGATCATCGTCCACGGCCACGTCTACGACTGCACCCGCTTCCTCAAAGACCACCCCGGCGGCTCCGACAGCATTCTGATCAACGCCGGCACCGACTGCACCGAAGAGTTCGACGCCATCCACTCCGACAAAGCCAAGAAAATGCTCGAAGACTACCGAATCGGCGAGTTGATCACCACCGGCTACGCCTCCGACTCCTCCTCCAACTCCCCCAACAACTCCACTCACGGCGCTTCCAATTTCTCCCACTTAGCCCCCATCAAGGAAATCACTCCTTCCTCGCCAATCCCAGCACGACGCGTGGCACTCGCCCCCAACCAGAAAATCCCCTGCAAGCTCGTGTCGAAGACCGCAATCTCACACGACGTGCGTGTCTTCAGATTCGCGCTGCCCGGTCAGGAGCAGACGCTGGGGCTGCCCGTGGGCAAGCACATTTTCATATGCGCCACGGTGGACGGCAAGCTCTGCATGAGGGCCTACACGCCCACGAGCTCAATAGACGAAGTGGGCTTCTTTGAATTGGTCGTCAAAGTCTACTTCAAGAACGTTCATCCGAAATTTCCCAACGGTGGAGTGATGTCGCAGTACTTGGAGTCGATGGAGGTGGGTTCGACGCTGGACGTGAAGGGTCCGCTGGGGCACATCGAGTACACGGGGCGGGGAAACTTCATGGTCCATGGGAAGCCCCGGTTCGCGAAGCGGCTGGCGATGCTGGCGGGTGGGACCGGGATAACGCCGATTTATCAAGTGGTGCAGGCGATATTGAAGGATCCGGAGGACGAGACGGAGATGTTTGTGGTGTATGCGAATCGGACGGAGGAGGATATTTTGCTGAGGGAGGAATTGGAGATGTGGGCCAAGAAGAATGACAGATTGAAAGTGTGGTACGTGGTGCAGGAGAGTATTAGAGAAGGCTGGGAATACAGCGTTGGGTTTATCACGGAGAATATTCTGAGAGAGCATATTCCGACGGCGGCGGAGGATACTTTGGCTCTCGTATGTGGGCCGCCGCCGATGATTCAGTTCGCGGTGCAACCCAATTTGGAGAAGATGAATTACGACACCAAGAATTCGATGCTGGTGTTTTAA

Coding sequence (CDS)

ATGGCGGCCTCCGTCGACAACCGCCAGTTCGGCCCTCTTCAGCCACCGCTCAACGGCGTCGTCAGGACCTTTAAGTCCGGTCCCAACCACCGCTCCGACTCTCCCGTCCGTGGCTGTAATTTCCCCAATTCCAACATCGACTACAGCAACGGCAACCGTTCCGTGAAAAGTTCTGTGAAGATAGAAGAGGAGGAGGATTCCAGTAGTGACGATGAGAATGAGATCAACGAGTTTCGTGAGTTGATCAAGAAGGGAAACCGTGAACTGGAGCCGTCGATTGTGGACCCCAGAGATGAAGGAACGGCTGATAATTGGATCGAGCGGAATTCTTCCATGGTCCGTCTCACCGGGAAGCACCCCTTCAACTCCGAGCCGCCTCTCAACCGTCTCATGCACCACGGCTTCATCACCCCGGTCCCACTCCACTACGTGCGCAACCACGGCGCGGTCCCCAAGGCCAAGTGGAGCGACTGGAGCATCGAGATCTGCGGGCTCGTCAAGCGCCCCGCCAAGTTCACCATGGACCAACTCGTCAACGAGTTTCGCTTCCGGGAGTTTCCGGCAACACTCGTCTGCGCCGGCAACCGGCGGAAGGAGCAGAACATGGTGAAAAAGACCATTGGGTTCAACTGGGGAGCGGCGGGGGTGTCCACGTCGGTCTGGCGAGGGGTCCCACTGCGTGACGTGTTGAAACGATGCGGGATTTTTAGCCGGAAGAAGGGCGCGCTGAATGTGTGCTTTGAAGGAGCGGAGGACTTGCCAGGTGGCGGTGGGTCCAAGTACGGGACCAGCATCAAGAAGGAGCTGGCTATGGATCCGGCTAGGGACATCATTTTGGCTTACATGCAGAACGGCGAACAGTTGTCGCCGGATCATGGATTTCCCGTGAGGATGATAATACCGGGATTCATTGGGGGTAGAATGGTAAAGTGGCTCAAACGGATCATTGTGACAACCAAAGAATCCGAAAATTATTACCATTACAAGGATAACAGAGTATTACCTTCACATGTCGACGCTGAGCTGGCCAACTCCGAAGCATGGTGGTACAAGCCTGAGTATATAATCAACGAGTTGAACATTAACTCCGTGATAACGACGCCCTGCCACGAGGAGATTTTACCCATCAACTCCTGGACCACACAGAGGCCGTACACCTTGAGAGGCTACTCTTATTCCGGTGGTGGGAAGAAGGTCACACGTGTGGAGGTTACAATGGACAGTGGAGAGACATGGCACGTGTGCACGTTGGACCATCCGGAGAAGCCAAACAAGTTTGGAAAATACTGGTGCTGGTGTTTCTGGTCGTTGGAAGTGGAGGTTCTGGACTTGCTTGGCGCCAAGGAAATCGCGGTTCGGGCTTGGGACGAAACCCTCAATACCCAACCCGAGAAACTCATCTGGAACCTCATGGGAATGATGAACAACTGTTGGTTCAGAGTGAAAACGAACGTATGCAAACCCCACAAGGGAGAGATCGGAATCATCTTCGAACACCCGACACTCCCGGGCAACCAGTCCGGCGGATGGATGGACAGAGAGAGACATCTGGAAATATCCTCCGAGTCCAACCAAACCCTGAAGAAGAGCGTCTCCACTCCATTCATGAACACCGCCTCCAACACCTACTCCTTATCGGAAGTCAAAAAGCACAACTCCCCTCAGTCCGCCTGGATCATCGTCCACGGCCACGTCTACGACTGCACCCGCTTCCTCAAAGACCACCCCGGCGGCTCCGACAGCATTCTGATCAACGCCGGCACCGACTGCACCGAAGAGTTCGACGCCATCCACTCCGACAAAGCCAAGAAAATGCTCGAAGACTACCGAATCGGCGAGTTGATCACCACCGGCTACGCCTCCGACTCCTCCTCCAACTCCCCCAACAACTCCACTCACGGCGCTTCCAATTTCTCCCACTTAGCCCCCATCAAGGAAATCACTCCTTCCTCGCCAATCCCAGCACGACGCGTGGCACTCGCCCCCAACCAGAAAATCCCCTGCAAGCTCGTGTCGAAGACCGCAATCTCACACGACGTGCGTGTCTTCAGATTCGCGCTGCCCGGTCAGGAGCAGACGCTGGGGCTGCCCGTGGGCAAGCACATTTTCATATGCGCCACGGTGGACGGCAAGCTCTGCATGAGGGCCTACACGCCCACGAGCTCAATAGACGAAGTGGGCTTCTTTGAATTGGTCGTCAAAGTCTACTTCAAGAACGTTCATCCGAAATTTCCCAACGGTGGAGTGATGTCGCAGTACTTGGAGTCGATGGAGGTGGGTTCGACGCTGGACGTGAAGGGTCCGCTGGGGCACATCGAGTACACGGGGCGGGGAAACTTCATGGTCCATGGGAAGCCCCGGTTCGCGAAGCGGCTGGCGATGCTGGCGGGTGGGACCGGGATAACGCCGATTTATCAAGTGGTGCAGGCGATATTGAAGGATCCGGAGGACGAGACGGAGATGTTTGTGGTGTATGCGAATCGGACGGAGGAGGATATTTTGCTGAGGGAGGAATTGGAGATGTGGGCCAAGAAGAATGACAGATTGAAAGTGTGGTACGTGGTGCAGGAGAGTATTAGAGAAGGCTGGGAATACAGCGTTGGGTTTATCACGGAGAATATTCTGAGAGAGCATATTCCGACGGCGGCGGAGGATACTTTGGCTCTCGTATGTGGGCCGCCGCCGATGATTCAGTTCGCGGTGCAACCCAATTTGGAGAAGATGAATTACGACACCAAGAATTCGATGCTGGTGTTTTAA

Protein sequence

MAASVDNRQFGPLQPPLNGVVRTFKSGPNHRSDSPVRGCNFPNSNIDYSNGNRSVKSSVKIEEEEDSSSDDENEINEFRELIKKGNRELEPSIVDPRDEGTADNWIERNSSMVRLTGKHPFNSEPPLNRLMHHGFITPVPLHYVRNHGAVPKAKWSDWSIEICGLVKRPAKFTMDQLVNEFRFREFPATLVCAGNRRKEQNMVKKTIGFNWGAAGVSTSVWRGVPLRDVLKRCGIFSRKKGALNVCFEGAEDLPGGGGSKYGTSIKKELAMDPARDIILAYMQNGEQLSPDHGFPVRMIIPGFIGGRMVKWLKRIIVTTKESENYYHYKDNRVLPSHVDAELANSEAWWYKPEYIINELNINSVITTPCHEEILPINSWTTQRPYTLRGYSYSGGGKKVTRVEVTMDSGETWHVCTLDHPEKPNKFGKYWCWCFWSLEVEVLDLLGAKEIAVRAWDETLNTQPEKLIWNLMGMMNNCWFRVKTNVCKPHKGEIGIIFEHPTLPGNQSGGWMDRERHLEISSESNQTLKKSVSTPFMNTASNTYSLSEVKKHNSPQSAWIIVHGHVYDCTRFLKDHPGGSDSILINAGTDCTEEFDAIHSDKAKKMLEDYRIGELITTGYASDSSSNSPNNSTHGASNFSHLAPIKEITPSSPIPARRVALAPNQKIPCKLVSKTAISHDVRVFRFALPGQEQTLGLPVGKHIFICATVDGKLCMRAYTPTSSIDEVGFFELVVKVYFKNVHPKFPNGGVMSQYLESMEVGSTLDVKGPLGHIEYTGRGNFMVHGKPRFAKRLAMLAGGTGITPIYQVVQAILKDPEDETEMFVVYANRTEEDILLREELEMWAKKNDRLKVWYVVQESIREGWEYSVGFITENILREHIPTAAEDTLALVCGPPPMIQFAVQPNLEKMNYDTKNSMLVF
Homology
BLAST of Spg008806 vs. NCBI nr
Match: XP_038907222.1 (nitrate reductase [NADH] [Benincasa hispida])

HSP 1 Score: 1786.5 bits (4626), Expect = 0.0e+00
Identity = 852/923 (92.31%), Postives = 891/923 (96.53%), Query Frame = 0

Query: 1   MAASVDNRQFGPLQPPLNGVVRTFKSGPNHRSDSPVRGCNFPNSNIDYSNGN-RSVKSSV 60
           MAASVDNRQFG LQPPLNGVVR+FKSGPNHRSDSPVRGCNFPNSNIDY+N N R +KSSV
Sbjct: 1   MAASVDNRQFGSLQPPLNGVVRSFKSGPNHRSDSPVRGCNFPNSNIDYNNNNGRLLKSSV 60

Query: 61  KI--EEEEDSSSDDENEINEFRELIKKGNRELEPSIVDPRDEGTADNWIERNSSMVRLTG 120
            I  EEEEDS S+DEN+  EFRELI+KGNRELEPSIVDPRDEGTADNWIERNSSMVRLTG
Sbjct: 61  NIQQEEEEDSCSEDENDNYEFRELIQKGNRELEPSIVDPRDEGTADNWIERNSSMVRLTG 120

Query: 121 KHPFNSEPPLNRLMHHGFITPVPLHYVRNHGAVPKAKWSDWSIEICGLVKRPAKFTMDQL 180
           KHPFNSEPPLNRLMHHGFITPVPLHYVRNHGAVP+AKWSDW+IE+CGLVKRP KFTMDQL
Sbjct: 121 KHPFNSEPPLNRLMHHGFITPVPLHYVRNHGAVPQAKWSDWTIEVCGLVKRPTKFTMDQL 180

Query: 181 VNEFRFREFPATLVCAGNRRKEQNMVKKTIGFNWGAAGVSTSVWRGVPLRDVLKRCGIFS 240
           VNEFRFREFPATLVCAGNRRKEQNMVKK+IGFNWGAAGVSTSVWRGVPLRDVLKRCGI S
Sbjct: 181 VNEFRFREFPATLVCAGNRRKEQNMVKKSIGFNWGAAGVSTSVWRGVPLRDVLKRCGIMS 240

Query: 241 RKKGALNVCFEGAEDLPGGGGSKYGTSIKKELAMDPARDIILAYMQNGEQLSPDHGFPVR 300
           RKKGALNVCFEGAEDLPGGGGSKYGTSIKKELAMDPARDIILAYMQNGEQL PDHGFPVR
Sbjct: 241 RKKGALNVCFEGAEDLPGGGGSKYGTSIKKELAMDPARDIILAYMQNGEQLVPDHGFPVR 300

Query: 301 MIIPGFIGGRMVKWLKRIIVTTKESENYYHYKDNRVLPSHVDAELANSEAWWYKPEYIIN 360
           MIIPGFIGGRMVKWLKRIIVTTKESENYYH+KDNRVLPSHVDAELAN+EAWWYKPEYIIN
Sbjct: 301 MIIPGFIGGRMVKWLKRIIVTTKESENYYHFKDNRVLPSHVDAELANAEAWWYKPEYIIN 360

Query: 361 ELNINSVITTPCHEEILPINSWTTQRPYTLRGYSYSGGGKKVTRVEVTMDSGETWHVCTL 420
           ELNINSVITTPCHEEILPINSWTTQRPYTLRGYSYSGGGKKVTRVEVTMD GETW VCTL
Sbjct: 361 ELNINSVITTPCHEEILPINSWTTQRPYTLRGYSYSGGGKKVTRVEVTMDGGETWQVCTL 420

Query: 421 DHPEKPNKFGKYWCWCFWSLEVEVLDLLGAKEIAVRAWDETLNTQPEKLIWNLMGMMNNC 480
           DHPEKPNK+GKYWCWCFWSLEVEVLDLLGAKEIAVRAWDETLNTQPEKLIWNLMGMMNNC
Sbjct: 421 DHPEKPNKYGKYWCWCFWSLEVEVLDLLGAKEIAVRAWDETLNTQPEKLIWNLMGMMNNC 480

Query: 481 WFRVKTNVCKPHKGEIGIIFEHPTLPGNQSGGWMDRERHLEISSESNQTLKKSVSTPFMN 540
           WFRVKTNVCKPHKGEIGIIFEHPT+PGNQSGGWMDRERHLE+SSESNQTLKKSVSTPFMN
Sbjct: 481 WFRVKTNVCKPHKGEIGIIFEHPTVPGNQSGGWMDRERHLEMSSESNQTLKKSVSTPFMN 540

Query: 541 TASNTYSLSEVKKHNSPQSAWIIVHGHVYDCTRFLKDHPGGSDSILINAGTDCTEEFDAI 600
           TASNTYSLSEVKKHNSPQSAWIIVHGHVYDCTRFLKDHPGGSDSILINAGTDCTEEFDAI
Sbjct: 541 TASNTYSLSEVKKHNSPQSAWIIVHGHVYDCTRFLKDHPGGSDSILINAGTDCTEEFDAI 600

Query: 601 HSDKAKKMLEDYRIGELITTGYASDSSSNSPNNSTHGASNFSHLAPIKEITPSSPIPARR 660
           HSDKAKKMLEDYRIGELITTGYASDSSS+SPNNSTHGASNFSHLAPIKE    +P+  RR
Sbjct: 601 HSDKAKKMLEDYRIGELITTGYASDSSSSSPNNSTHGASNFSHLAPIKE----APMLTRR 660

Query: 661 VALAPNQKIPCKLVSKTAISHDVRVFRFALP-GQEQTLGLPVGKHIFICATVDGKLCMRA 720
           VAL PN+KIPCKL+SKTAISHDVRVFRF LP G +Q+LGLPVGKHIFICA VDGKLCMRA
Sbjct: 661 VALTPNEKIPCKLISKTAISHDVRVFRFELPGGLDQSLGLPVGKHIFICAKVDGKLCMRA 720

Query: 721 YTPTSSIDEVGFFELVVKVYFKNVHPKFPNGGVMSQYLESMEVGSTLDVKGPLGHIEYTG 780
           YTP++ ID+ G+FELVVKVYFKNVHPKFPNGG+MSQYL++MEVGS++++KGPLGHIEYTG
Sbjct: 721 YTPSTPIDQTGYFELVVKVYFKNVHPKFPNGGLMSQYLDNMEVGSSVEIKGPLGHIEYTG 780

Query: 781 RGNFMVHGKPRFAKRLAMLAGGTGITPIYQVVQAILKDPEDETEMFVVYANRTEEDILLR 840
           RGNF VHGKPRFAKRLAMLAGGTGITPIYQVVQAILKDPEDETEMFVVYANRTE+DILLR
Sbjct: 781 RGNFTVHGKPRFAKRLAMLAGGTGITPIYQVVQAILKDPEDETEMFVVYANRTEDDILLR 840

Query: 841 EELEMWAKKNDRLKVWYVVQESIREGWEYSVGFITENILREHIPTAAEDTLALVCGPPPM 900
           EEL+ WAKKN+RLKVWYVVQESIREGWEYS GFITE+I+R+HIP AAEDTLALVCGPPPM
Sbjct: 841 EELDTWAKKNERLKVWYVVQESIREGWEYSTGFITEDIMRKHIPAAAEDTLALVCGPPPM 900

Query: 901 IQFAVQPNLEKMNYDTKNSMLVF 920
           IQFAVQPNLEKMNYDTKNSMLVF
Sbjct: 901 IQFAVQPNLEKMNYDTKNSMLVF 919

BLAST of Spg008806 vs. NCBI nr
Match: NP_001267696.1 (nitrate reductase [NADH]-like [Cucumis sativus] >ADN96689.1 nitrate reductase 2 [Cucumis sativus])

HSP 1 Score: 1780.0 bits (4609), Expect = 0.0e+00
Identity = 845/923 (91.55%), Postives = 888/923 (96.21%), Query Frame = 0

Query: 1   MAASVDNRQFGPLQPPLNGVVRTFKSGPNHRSDSPVRGCNFPNSNIDYSNGNRSVKSSVK 60
           MAASVDNRQFG LQPPLNGVVR+FKSGPNHRSDSPVRGCNFPNSNI+    NR +KSSVK
Sbjct: 1   MAASVDNRQFGSLQPPLNGVVRSFKSGPNHRSDSPVRGCNFPNSNIN----NRLLKSSVK 60

Query: 61  IEEEE---DSSSDDENEINEFRELIKKGNRELEPSIVDPRDEGTADNWIERNSSMVRLTG 120
           +E+EE   DS S+DEN+ NE RELIKKGNRELEPS VDPRDEGTADNWIERNSSMVRLTG
Sbjct: 61  MEQEEEDDDSCSEDENDNNELRELIKKGNRELEPSTVDPRDEGTADNWIERNSSMVRLTG 120

Query: 121 KHPFNSEPPLNRLMHHGFITPVPLHYVRNHGAVPKAKWSDWSIEICGLVKRPAKFTMDQL 180
           KHPFNSEPPLNRLMHHGFITPVPLHYVRNHGAVPKAKWSDW+IE+CGLVKRP KF+MDQL
Sbjct: 121 KHPFNSEPPLNRLMHHGFITPVPLHYVRNHGAVPKAKWSDWTIEVCGLVKRPTKFSMDQL 180

Query: 181 VNEFRFREFPATLVCAGNRRKEQNMVKKTIGFNWGAAGVSTSVWRGVPLRDVLKRCGIFS 240
           VNEFRFREFPATLVCAGNRRKEQNMVKK+IGFNWGAAGVSTSVWRGVPLR+VLKRCGI S
Sbjct: 181 VNEFRFREFPATLVCAGNRRKEQNMVKKSIGFNWGAAGVSTSVWRGVPLREVLKRCGIMS 240

Query: 241 RKKGALNVCFEGAEDLPGGGGSKYGTSIKKELAMDPARDIILAYMQNGEQLSPDHGFPVR 300
           RKKGALNVCFEGAEDLPGGGGSKYGTSIKKELAMDPARDIILAYMQNGEQL+PDHGFPVR
Sbjct: 241 RKKGALNVCFEGAEDLPGGGGSKYGTSIKKELAMDPARDIILAYMQNGEQLAPDHGFPVR 300

Query: 301 MIIPGFIGGRMVKWLKRIIVTTKESENYYHYKDNRVLPSHVDAELANSEAWWYKPEYIIN 360
           MIIPGFIGGRMVKWLKRIIVTTKESENYYH+KDNRVLPSHVDAELAN+EAWWYKPEYIIN
Sbjct: 301 MIIPGFIGGRMVKWLKRIIVTTKESENYYHFKDNRVLPSHVDAELANAEAWWYKPEYIIN 360

Query: 361 ELNINSVITTPCHEEILPINSWTTQRPYTLRGYSYSGGGKKVTRVEVTMDSGETWHVCTL 420
           ELNINSVITTPCHEEILPINSWTTQRPYTLRGYSYSGGGKKVTRVEVTMD GETW VCTL
Sbjct: 361 ELNINSVITTPCHEEILPINSWTTQRPYTLRGYSYSGGGKKVTRVEVTMDGGETWQVCTL 420

Query: 421 DHPEKPNKFGKYWCWCFWSLEVEVLDLLGAKEIAVRAWDETLNTQPEKLIWNLMGMMNNC 480
           DHPEKPNK+GKYWCWCFWSLEVEVLDLLGAKEIAVRAWDETLNTQPEKLIWNLMGMMNNC
Sbjct: 421 DHPEKPNKYGKYWCWCFWSLEVEVLDLLGAKEIAVRAWDETLNTQPEKLIWNLMGMMNNC 480

Query: 481 WFRVKTNVCKPHKGEIGIIFEHPTLPGNQSGGWMDRERHLEISSESNQTLKKSVSTPFMN 540
           WF VKTNVCKPHKGEIGIIFEHPT+PGNQSGGWMDRERHLEIS+ESNQTLKKS+STPFMN
Sbjct: 481 WFTVKTNVCKPHKGEIGIIFEHPTVPGNQSGGWMDRERHLEISTESNQTLKKSISTPFMN 540

Query: 541 TASNTYSLSEVKKHNSPQSAWIIVHGHVYDCTRFLKDHPGGSDSILINAGTDCTEEFDAI 600
           TASNTYSLSEVKKHNSPQSAWIIVHGHVYDCTRFLKDHPGGSDSILINAGTDCTEEFDAI
Sbjct: 541 TASNTYSLSEVKKHNSPQSAWIIVHGHVYDCTRFLKDHPGGSDSILINAGTDCTEEFDAI 600

Query: 601 HSDKAKKMLEDYRIGELITTGYASDSSSNSPNNSTHGASNFSHLAPIKEITPSSPIPARR 660
           HSDKAKKMLEDYRIGELITTGYASDSSSNSPNNSTHGASNFSHLAPI+E    +P+  RR
Sbjct: 601 HSDKAKKMLEDYRIGELITTGYASDSSSNSPNNSTHGASNFSHLAPIRE----APMVTRR 660

Query: 661 VALAPNQKIPCKLVSKTAISHDVRVFRFALP-GQEQTLGLPVGKHIFICATVDGKLCMRA 720
            ALAPN+KIPCKL+SKT ISHDVRVFRF LP GQ+Q LGLPVGKHIFICA VDGKLCMRA
Sbjct: 661 AALAPNEKIPCKLISKTEISHDVRVFRFELPGGQDQVLGLPVGKHIFICAKVDGKLCMRA 720

Query: 721 YTPTSSIDEVGFFELVVKVYFKNVHPKFPNGGVMSQYLESMEVGSTLDVKGPLGHIEYTG 780
           YTP+S++D++G+FELVVKVYFKNVHPKFPNGG+MSQ+L++MEVGST++VKGPLGHIEYTG
Sbjct: 721 YTPSSTVDQMGYFELVVKVYFKNVHPKFPNGGIMSQFLDNMEVGSTVEVKGPLGHIEYTG 780

Query: 781 RGNFMVHGKPRFAKRLAMLAGGTGITPIYQVVQAILKDPEDETEMFVVYANRTEEDILLR 840
           RGNF VHGKPRFAKRLAMLAGGTGITPIYQ+VQAILKDPEDETEMFVVYANRTE+DILLR
Sbjct: 781 RGNFTVHGKPRFAKRLAMLAGGTGITPIYQIVQAILKDPEDETEMFVVYANRTEDDILLR 840

Query: 841 EELEMWAKKNDRLKVWYVVQESIREGWEYSVGFITENILREHIPTAAEDTLALVCGPPPM 900
           EEL+ WAKKN+RLKVWYVVQESIREGWEYS+GFITE I REH+P AAEDTLALVCGPPPM
Sbjct: 841 EELDTWAKKNERLKVWYVVQESIREGWEYSIGFITEEITREHLPAAAEDTLALVCGPPPM 900

Query: 901 IQFAVQPNLEKMNYDTKNSMLVF 920
           IQFAVQPNLEKMNYDTKNSMLVF
Sbjct: 901 IQFAVQPNLEKMNYDTKNSMLVF 915

BLAST of Spg008806 vs. NCBI nr
Match: TYK26501.1 (nitrate reductase [Cucumis melo var. makuwa])

HSP 1 Score: 1777.3 bits (4602), Expect = 0.0e+00
Identity = 845/923 (91.55%), Postives = 887/923 (96.10%), Query Frame = 0

Query: 1   MAASVDNRQFGPLQPPLNGVVRTFKSGPNHRSDSPVRGCNFPNSNIDYSNGNRSVKSSVK 60
           MAASVDNRQFG LQPPLNGVVR+FKSGPN RSDSPVRGCNFPNSNI+    NR +KSSVK
Sbjct: 1   MAASVDNRQFGSLQPPLNGVVRSFKSGPNRRSDSPVRGCNFPNSNIN----NRLLKSSVK 60

Query: 61  IEEEE---DSSSDDENEINEFRELIKKGNRELEPSIVDPRDEGTADNWIERNSSMVRLTG 120
           +E+EE   DS S+DEN+ NE RELIKKGNRELEPSIVDPRDE TADNWIERNSSMVRLTG
Sbjct: 61  MEQEEEDDDSCSEDENDNNELRELIKKGNRELEPSIVDPRDEATADNWIERNSSMVRLTG 120

Query: 121 KHPFNSEPPLNRLMHHGFITPVPLHYVRNHGAVPKAKWSDWSIEICGLVKRPAKFTMDQL 180
           KHPFNSEPPLNRLMHHGFITPVPLHYVRNHGAVPKA WSDW+IE+CGLVKRP KFTMDQL
Sbjct: 121 KHPFNSEPPLNRLMHHGFITPVPLHYVRNHGAVPKANWSDWTIEVCGLVKRPTKFTMDQL 180

Query: 181 VNEFRFREFPATLVCAGNRRKEQNMVKKTIGFNWGAAGVSTSVWRGVPLRDVLKRCGIFS 240
           VNEFRFREFPATLVCAGNRRKEQNMVKK+IGFNWGAAGVSTSVWRGVPLRDVLKRCGI S
Sbjct: 181 VNEFRFREFPATLVCAGNRRKEQNMVKKSIGFNWGAAGVSTSVWRGVPLRDVLKRCGIMS 240

Query: 241 RKKGALNVCFEGAEDLPGGGGSKYGTSIKKELAMDPARDIILAYMQNGEQLSPDHGFPVR 300
           RKKGALNVCFEGAEDLPGGGGSKYGTSIKKELAMDPARDIILAYMQNGEQL PDHGFPVR
Sbjct: 241 RKKGALNVCFEGAEDLPGGGGSKYGTSIKKELAMDPARDIILAYMQNGEQLVPDHGFPVR 300

Query: 301 MIIPGFIGGRMVKWLKRIIVTTKESENYYHYKDNRVLPSHVDAELANSEAWWYKPEYIIN 360
           MIIPGFIGGRMVKWLKRIIVTTKESENYYH+KDNRVLPSHVDAELAN+EAWWYKPEYIIN
Sbjct: 301 MIIPGFIGGRMVKWLKRIIVTTKESENYYHFKDNRVLPSHVDAELANAEAWWYKPEYIIN 360

Query: 361 ELNINSVITTPCHEEILPINSWTTQRPYTLRGYSYSGGGKKVTRVEVTMDSGETWHVCTL 420
           ELNINSVITTPCHEEILPINSWTTQRPYTLRGYSYSGGGKKVTRVEVTMD GETW VCTL
Sbjct: 361 ELNINSVITTPCHEEILPINSWTTQRPYTLRGYSYSGGGKKVTRVEVTMDGGETWQVCTL 420

Query: 421 DHPEKPNKFGKYWCWCFWSLEVEVLDLLGAKEIAVRAWDETLNTQPEKLIWNLMGMMNNC 480
           DHPEKPNK+GKYWCWCFWSLEVEVLDLLGAKEIAVRAWDETLNTQPEKLIWNLMGMMNNC
Sbjct: 421 DHPEKPNKYGKYWCWCFWSLEVEVLDLLGAKEIAVRAWDETLNTQPEKLIWNLMGMMNNC 480

Query: 481 WFRVKTNVCKPHKGEIGIIFEHPTLPGNQSGGWMDRERHLEISSESNQTLKKSVSTPFMN 540
           WF VKTNVCKPHKGEIGIIFEHPT+PGNQSGGWMDRERHLEIS+ESNQTLKKS+STPFMN
Sbjct: 481 WFTVKTNVCKPHKGEIGIIFEHPTVPGNQSGGWMDRERHLEISTESNQTLKKSISTPFMN 540

Query: 541 TASNTYSLSEVKKHNSPQSAWIIVHGHVYDCTRFLKDHPGGSDSILINAGTDCTEEFDAI 600
           TASNTYSLSEVKKHNSPQSAWIIVHGHVYDCTRFLKDHPGGSDSILINAGTDCTEEFDAI
Sbjct: 541 TASNTYSLSEVKKHNSPQSAWIIVHGHVYDCTRFLKDHPGGSDSILINAGTDCTEEFDAI 600

Query: 601 HSDKAKKMLEDYRIGELITTGYASDSSSNSPNNSTHGASNFSHLAPIKEITPSSPIPARR 660
           HSDKAKKMLEDYRIGELITTGYASDSSSNSPNNSTHGASNFSHLAPI+E    +P+  RR
Sbjct: 601 HSDKAKKMLEDYRIGELITTGYASDSSSNSPNNSTHGASNFSHLAPIRE----APVVTRR 660

Query: 661 VALAPNQKIPCKLVSKTAISHDVRVFRFALP-GQEQTLGLPVGKHIFICATVDGKLCMRA 720
           VALAPN+KIPCKL+SKTAISHDVRVFRF LP GQ+Q LGLPVGKHIFICA V+GKLCMRA
Sbjct: 661 VALAPNEKIPCKLISKTAISHDVRVFRFELPGGQDQVLGLPVGKHIFICAKVEGKLCMRA 720

Query: 721 YTPTSSIDEVGFFELVVKVYFKNVHPKFPNGGVMSQYLESMEVGSTLDVKGPLGHIEYTG 780
           YTP++++D++G+FELVVKVYFKNVHPKFPNGG+MSQ+L++MEVGST++VKGPLGHIEYTG
Sbjct: 721 YTPSTTVDQMGYFELVVKVYFKNVHPKFPNGGIMSQFLDNMEVGSTVEVKGPLGHIEYTG 780

Query: 781 RGNFMVHGKPRFAKRLAMLAGGTGITPIYQVVQAILKDPEDETEMFVVYANRTEEDILLR 840
            GNF VHGKPRFAKRLAMLAGGTGITPIYQVVQAILKDPEDETEMFVVYANRTE+DILLR
Sbjct: 781 SGNFTVHGKPRFAKRLAMLAGGTGITPIYQVVQAILKDPEDETEMFVVYANRTEDDILLR 840

Query: 841 EELEMWAKKNDRLKVWYVVQESIREGWEYSVGFITENILREHIPTAAEDTLALVCGPPPM 900
           EEL+ WAKKN+RLKVWYVVQESIREGWEYS+GFITE I+REH+P AAEDTLALVCGPPPM
Sbjct: 841 EELDTWAKKNERLKVWYVVQESIREGWEYSIGFITEEIMREHLPAAAEDTLALVCGPPPM 900

Query: 901 IQFAVQPNLEKMNYDTKNSMLVF 920
           IQFAVQPNLEKMNYDTKNSMLVF
Sbjct: 901 IQFAVQPNLEKMNYDTKNSMLVF 915

BLAST of Spg008806 vs. NCBI nr
Match: XP_008460453.1 (PREDICTED: nitrate reductase [NADH] [Cucumis melo] >KAA0057717.1 nitrate reductase [Cucumis melo var. makuwa])

HSP 1 Score: 1776.1 bits (4599), Expect = 0.0e+00
Identity = 844/923 (91.44%), Postives = 887/923 (96.10%), Query Frame = 0

Query: 1   MAASVDNRQFGPLQPPLNGVVRTFKSGPNHRSDSPVRGCNFPNSNIDYSNGNRSVKSSVK 60
           MAASVDNRQFG LQPPLNGVVR+FKSGPN RSDSPVRGCNFPNSNI+    NR +KSSVK
Sbjct: 1   MAASVDNRQFGSLQPPLNGVVRSFKSGPNRRSDSPVRGCNFPNSNIN----NRLLKSSVK 60

Query: 61  IEEEE---DSSSDDENEINEFRELIKKGNRELEPSIVDPRDEGTADNWIERNSSMVRLTG 120
           +E+EE   DS S+DEN+ NE RELIKKGNRELEPSIVDPRDE TADNWIERNSSMVRLTG
Sbjct: 61  MEQEEEDDDSCSEDENDNNELRELIKKGNRELEPSIVDPRDEATADNWIERNSSMVRLTG 120

Query: 121 KHPFNSEPPLNRLMHHGFITPVPLHYVRNHGAVPKAKWSDWSIEICGLVKRPAKFTMDQL 180
           KHPFNSEPPLNRLMHHGFITPVPLHYVRNHGAVPKA WSDW+IE+CGLVKRP KFTMDQL
Sbjct: 121 KHPFNSEPPLNRLMHHGFITPVPLHYVRNHGAVPKANWSDWTIEVCGLVKRPTKFTMDQL 180

Query: 181 VNEFRFREFPATLVCAGNRRKEQNMVKKTIGFNWGAAGVSTSVWRGVPLRDVLKRCGIFS 240
           VNEFRFREFPATLVCAGNRRKEQNMVKK+IGFNWGAAGVSTSVWRGVPLRDVLKRCGI S
Sbjct: 181 VNEFRFREFPATLVCAGNRRKEQNMVKKSIGFNWGAAGVSTSVWRGVPLRDVLKRCGIMS 240

Query: 241 RKKGALNVCFEGAEDLPGGGGSKYGTSIKKELAMDPARDIILAYMQNGEQLSPDHGFPVR 300
           RKKGALNVCFEGAEDLPGGGGSKYGTSIKKELAMDPARDIILAYMQNGEQL PDHGFPVR
Sbjct: 241 RKKGALNVCFEGAEDLPGGGGSKYGTSIKKELAMDPARDIILAYMQNGEQLVPDHGFPVR 300

Query: 301 MIIPGFIGGRMVKWLKRIIVTTKESENYYHYKDNRVLPSHVDAELANSEAWWYKPEYIIN 360
           MIIPGFIGGRMVKWLKRIIVTTKESENYYH+KDNRVLPSHVDAELAN+EAWWYKPEYIIN
Sbjct: 301 MIIPGFIGGRMVKWLKRIIVTTKESENYYHFKDNRVLPSHVDAELANAEAWWYKPEYIIN 360

Query: 361 ELNINSVITTPCHEEILPINSWTTQRPYTLRGYSYSGGGKKVTRVEVTMDSGETWHVCTL 420
           ELNINSVITTPCHEEILPINSWTTQRPYTLRGYSYSGGGKKVTRVEVTMD GETW VCTL
Sbjct: 361 ELNINSVITTPCHEEILPINSWTTQRPYTLRGYSYSGGGKKVTRVEVTMDGGETWQVCTL 420

Query: 421 DHPEKPNKFGKYWCWCFWSLEVEVLDLLGAKEIAVRAWDETLNTQPEKLIWNLMGMMNNC 480
           DHPEKPNK+GKYWCWCFWSLEVEVLDLLGAKEIAVRAWDETLNTQPEKLIWNLMGMMNNC
Sbjct: 421 DHPEKPNKYGKYWCWCFWSLEVEVLDLLGAKEIAVRAWDETLNTQPEKLIWNLMGMMNNC 480

Query: 481 WFRVKTNVCKPHKGEIGIIFEHPTLPGNQSGGWMDRERHLEISSESNQTLKKSVSTPFMN 540
           WF VKTNVCKPHKGEIGIIFEHPT+PGNQSGGWMDRERHLEIS+ESNQTLKKS+STPFMN
Sbjct: 481 WFTVKTNVCKPHKGEIGIIFEHPTVPGNQSGGWMDRERHLEISTESNQTLKKSISTPFMN 540

Query: 541 TASNTYSLSEVKKHNSPQSAWIIVHGHVYDCTRFLKDHPGGSDSILINAGTDCTEEFDAI 600
           TASNTYSLSEVKKHNSPQSAWIIVHGHVYDCTRFLKDHPGGSDSILINAGTDCTEEFDAI
Sbjct: 541 TASNTYSLSEVKKHNSPQSAWIIVHGHVYDCTRFLKDHPGGSDSILINAGTDCTEEFDAI 600

Query: 601 HSDKAKKMLEDYRIGELITTGYASDSSSNSPNNSTHGASNFSHLAPIKEITPSSPIPARR 660
           HSDKAKKMLEDYRIGELITTGYASDSSSNSPNNSTHGASNFSHLAPI+E    +P+  RR
Sbjct: 601 HSDKAKKMLEDYRIGELITTGYASDSSSNSPNNSTHGASNFSHLAPIRE----APVVTRR 660

Query: 661 VALAPNQKIPCKLVSKTAISHDVRVFRFALP-GQEQTLGLPVGKHIFICATVDGKLCMRA 720
           VALAPN+KIPCKL+SKTAISHDVRVFRF LP GQ+Q LGLPVGKHIFICA V+GKLCMRA
Sbjct: 661 VALAPNEKIPCKLISKTAISHDVRVFRFELPGGQDQVLGLPVGKHIFICAKVEGKLCMRA 720

Query: 721 YTPTSSIDEVGFFELVVKVYFKNVHPKFPNGGVMSQYLESMEVGSTLDVKGPLGHIEYTG 780
           YTP++++D++G+FELVVKVYF+NVHPKFPNGG+MSQ+L++MEVGST++VKGPLGHIEYTG
Sbjct: 721 YTPSTTVDQMGYFELVVKVYFRNVHPKFPNGGIMSQFLDNMEVGSTVEVKGPLGHIEYTG 780

Query: 781 RGNFMVHGKPRFAKRLAMLAGGTGITPIYQVVQAILKDPEDETEMFVVYANRTEEDILLR 840
            GNF VHGKPRFAKRLAMLAGGTGITPIYQVVQAILKDPEDETEMFVVYANRTE+DILLR
Sbjct: 781 SGNFTVHGKPRFAKRLAMLAGGTGITPIYQVVQAILKDPEDETEMFVVYANRTEDDILLR 840

Query: 841 EELEMWAKKNDRLKVWYVVQESIREGWEYSVGFITENILREHIPTAAEDTLALVCGPPPM 900
           EEL+ WAKKN+RLKVWYVVQESIREGWEYS+GFITE I+REH+P AAEDTLALVCGPPPM
Sbjct: 841 EELDTWAKKNERLKVWYVVQESIREGWEYSIGFITEEIMREHLPAAAEDTLALVCGPPPM 900

Query: 901 IQFAVQPNLEKMNYDTKNSMLVF 920
           IQFAVQPNLEKMNYDTKNSMLVF
Sbjct: 901 IQFAVQPNLEKMNYDTKNSMLVF 915

BLAST of Spg008806 vs. NCBI nr
Match: ADK77877.1 (nitrate reductase [Cucumis sativus])

HSP 1 Score: 1773.4 bits (4592), Expect = 0.0e+00
Identity = 843/923 (91.33%), Postives = 887/923 (96.10%), Query Frame = 0

Query: 1   MAASVDNRQFGPLQPPLNGVVRTFKSGPNHRSDSPVRGCNFPNSNIDYSNGNRSVKSSVK 60
           MAASVDNRQFG LQPPLNGVVR+FKSGPNHRSDSPVRGCNFPNSNI+    NR +KSSVK
Sbjct: 1   MAASVDNRQFGSLQPPLNGVVRSFKSGPNHRSDSPVRGCNFPNSNIN----NRLLKSSVK 60

Query: 61  IEEEE---DSSSDDENEINEFRELIKKGNRELEPSIVDPRDEGTADNWIERNSSMVRLTG 120
           +E+EE   DS S+DEN+ NE RELIKKGNRELEPS VDPRDEGTADNWIERNSSMVRLTG
Sbjct: 61  MEQEEEDDDSCSEDENDNNELRELIKKGNRELEPSTVDPRDEGTADNWIERNSSMVRLTG 120

Query: 121 KHPFNSEPPLNRLMHHGFITPVPLHYVRNHGAVPKAKWSDWSIEICGLVKRPAKFTMDQL 180
           KHPFNSEPPLNRLMHHGFITPVPLHYVRNHGAVPKAKW+DW+IE+CGLVKRP KF+MDQL
Sbjct: 121 KHPFNSEPPLNRLMHHGFITPVPLHYVRNHGAVPKAKWADWTIEVCGLVKRPTKFSMDQL 180

Query: 181 VNEFRFREFPATLVCAGNRRKEQNMVKKTIGFNWGAAGVSTSVWRGVPLRDVLKRCGIFS 240
           VNEFRFREFPATLVCAGNRRKEQNMVKK+IGFNWGAAGVSTSVWRGVPLR+VLKRCGI S
Sbjct: 181 VNEFRFREFPATLVCAGNRRKEQNMVKKSIGFNWGAAGVSTSVWRGVPLREVLKRCGIMS 240

Query: 241 RKKGALNVCFEGAEDLPGGGGSKYGTSIKKELAMDPARDIILAYMQNGEQLSPDHGFPVR 300
           RKKGALNVCFEGAEDLPGGGGSKYGTSIKKELAMDPARDIILAYMQNGEQL+PDHGFPVR
Sbjct: 241 RKKGALNVCFEGAEDLPGGGGSKYGTSIKKELAMDPARDIILAYMQNGEQLAPDHGFPVR 300

Query: 301 MIIPGFIGGRMVKWLKRIIVTTKESENYYHYKDNRVLPSHVDAELANSEAWWYKPEYIIN 360
           MIIPGFIGGRMVKWLKRIIVTTKESENYYH+KDNRVLPSHVDAELAN+EAWWYKPEYIIN
Sbjct: 301 MIIPGFIGGRMVKWLKRIIVTTKESENYYHFKDNRVLPSHVDAELANAEAWWYKPEYIIN 360

Query: 361 ELNINSVITTPCHEEILPINSWTTQRPYTLRGYSYSGGGKKVTRVEVTMDSGETWHVCTL 420
           ELNINSVITTPCHEEILPINSWTTQRPYTLRGYSYSGGGKKVTRVEVTMD GET  VCTL
Sbjct: 361 ELNINSVITTPCHEEILPINSWTTQRPYTLRGYSYSGGGKKVTRVEVTMDGGETRQVCTL 420

Query: 421 DHPEKPNKFGKYWCWCFWSLEVEVLDLLGAKEIAVRAWDETLNTQPEKLIWNLMGMMNNC 480
           DHPEKPNK+GKYWCWCFWSLEVEVLDLLGAKEIAVRAWDETLNTQPEKLIWNLMGMMNNC
Sbjct: 421 DHPEKPNKYGKYWCWCFWSLEVEVLDLLGAKEIAVRAWDETLNTQPEKLIWNLMGMMNNC 480

Query: 481 WFRVKTNVCKPHKGEIGIIFEHPTLPGNQSGGWMDRERHLEISSESNQTLKKSVSTPFMN 540
           WF VKTNVCKPHKGEIGIIFEHPT+PGNQSGGWMDRERHLEIS+ESNQTLKKS+STPFMN
Sbjct: 481 WFTVKTNVCKPHKGEIGIIFEHPTVPGNQSGGWMDRERHLEISTESNQTLKKSISTPFMN 540

Query: 541 TASNTYSLSEVKKHNSPQSAWIIVHGHVYDCTRFLKDHPGGSDSILINAGTDCTEEFDAI 600
           TASNTYSLSEVKKHNSPQSAWIIVHGHVYDCTRFLKDHPGGSDSILINAGTDCTEEFDAI
Sbjct: 541 TASNTYSLSEVKKHNSPQSAWIIVHGHVYDCTRFLKDHPGGSDSILINAGTDCTEEFDAI 600

Query: 601 HSDKAKKMLEDYRIGELITTGYASDSSSNSPNNSTHGASNFSHLAPIKEITPSSPIPARR 660
           HSDKAKKMLEDYRIGELITTGYASDSSSNSPNNSTHGASNFSHLAPI+E    +P+  RR
Sbjct: 601 HSDKAKKMLEDYRIGELITTGYASDSSSNSPNNSTHGASNFSHLAPIRE----APMVTRR 660

Query: 661 VALAPNQKIPCKLVSKTAISHDVRVFRFALP-GQEQTLGLPVGKHIFICATVDGKLCMRA 720
            ALAPN+KIPCKL+SKT ISHDVRVFRF LP GQ+Q LGLPVGKHIFICA VDGKLCMRA
Sbjct: 661 AALAPNEKIPCKLISKTEISHDVRVFRFELPGGQDQVLGLPVGKHIFICAKVDGKLCMRA 720

Query: 721 YTPTSSIDEVGFFELVVKVYFKNVHPKFPNGGVMSQYLESMEVGSTLDVKGPLGHIEYTG 780
           YTP+S++D++G+FELVVKVYFKNVHPKFPNGG+MSQ+L++MEVGST++VKGPLGHIEYTG
Sbjct: 721 YTPSSTVDQMGYFELVVKVYFKNVHPKFPNGGIMSQFLDNMEVGSTVEVKGPLGHIEYTG 780

Query: 781 RGNFMVHGKPRFAKRLAMLAGGTGITPIYQVVQAILKDPEDETEMFVVYANRTEEDILLR 840
           RGNF VHGKPRFAKRLAMLAGGTGITPIYQ+VQAILKDPEDETEMFVVYANRTE+DILLR
Sbjct: 781 RGNFTVHGKPRFAKRLAMLAGGTGITPIYQIVQAILKDPEDETEMFVVYANRTEDDILLR 840

Query: 841 EELEMWAKKNDRLKVWYVVQESIREGWEYSVGFITENILREHIPTAAEDTLALVCGPPPM 900
           EEL+ WAKKN+RLKVWYVVQESIREGWEYS+GFITE I REH+P AAEDTLALVCGPPPM
Sbjct: 841 EELDTWAKKNERLKVWYVVQESIREGWEYSIGFITEEITREHLPAAAEDTLALVCGPPPM 900

Query: 901 IQFAVQPNLEKMNYDTKNSMLVF 920
           IQFAVQPNLEKMNYDTKNSMLVF
Sbjct: 901 IQFAVQPNLEKMNYDTKNSMLVF 915

BLAST of Spg008806 vs. ExPASy Swiss-Prot
Match: P17569 (Nitrate reductase [NADH] OS=Cucurbita maxima OX=3661 PE=2 SV=1)

HSP 1 Score: 1765.7 bits (4572), Expect = 0.0e+00
Identity = 842/925 (91.03%), Postives = 891/925 (96.32%), Query Frame = 0

Query: 1   MAASVDNRQFGPLQPPLNGVVRTFKSGPNHRSDSPVRGCNFPNSNIDYSNGNRSVKSSVK 60
           MAASVDNRQ+GPLQPPL+GVVR+FK+GPNHR+DSPVRGCNFPNSN+DY+N NR +KSSVK
Sbjct: 1   MAASVDNRQYGPLQPPLSGVVRSFKNGPNHRADSPVRGCNFPNSNVDYNN-NRPLKSSVK 60

Query: 61  I-----EEEEDSSSDDENEINEFRELIKKGNRELEPSIVDPRDEGTADNWIERNSSMVRL 120
           I     EE EDS S+DENE NEFR+LI KGNRELEPSI+D RDEGTADNWIERN+SMVRL
Sbjct: 61  IQEAAAEEMEDSCSEDENE-NEFRDLIVKGNRELEPSILDHRDEGTADNWIERNASMVRL 120

Query: 121 TGKHPFNSEPPLNRLMHHGFITPVPLHYVRNHGAVPKAKWSDWSIEICGLVKRPAKFTMD 180
           TGKHPFNSEPPLNRLMHHGFITPVPLHYVRNHG VPKAKW+DW++E+CGLVKRPAKFTMD
Sbjct: 121 TGKHPFNSEPPLNRLMHHGFITPVPLHYVRNHGVVPKAKWADWTVEVCGLVKRPAKFTMD 180

Query: 181 QLVNEFRFREFPATLVCAGNRRKEQNMVKKTIGFNWGAAGVSTSVWRGVPLRDVLKRCGI 240
           QL+NEFRFREFPATLVCAGNRRKEQNMVK++IGFNWGAAGVSTSVWR VPL D+LKRCGI
Sbjct: 181 QLLNEFRFREFPATLVCAGNRRKEQNMVKQSIGFNWGAAGVSTSVWRRVPLCDLLKRCGI 240

Query: 241 FSRKKGALNVCFEGAEDLPGGGGSKYGTSIKKELAMDPARDIILAYMQNGEQLSPDHGFP 300
            SRKKGALNVCFEGAEDLPGGGGSKYGTSIKKELAMDPARDIILAYMQNGEQL+PDHGFP
Sbjct: 241 LSRKKGALNVCFEGAEDLPGGGGSKYGTSIKKELAMDPARDIILAYMQNGEQLAPDHGFP 300

Query: 301 VRMIIPGFIGGRMVKWLKRIIVTTKESENYYHYKDNRVLPSHVDAELANSEAWWYKPEYI 360
           VRMIIPGFIGGRMVKWLKRIIVTTKESENYYH+KDNRVLPSHVDA++AN+EAWWYKPE+I
Sbjct: 301 VRMIIPGFIGGRMVKWLKRIIVTTKESENYYHFKDNRVLPSHVDADVANAEAWWYKPEHI 360

Query: 361 INELNINSVITTPCHEEILPINSWTTQRPYTLRGYSYSGGGKKVTRVEVTMDSGETWHVC 420
           INELNINSVITTPCHEEILPIN+WTTQRPYTLRGYSYSGGGKKVTRVEVTMDSGETW VC
Sbjct: 361 INELNINSVITTPCHEEILPINAWTTQRPYTLRGYSYSGGGKKVTRVEVTMDSGETWQVC 420

Query: 421 TLDHPEKPNKFGKYWCWCFWSLEVEVLDLLGAKEIAVRAWDETLNTQPEKLIWNLMGMMN 480
           TLDHPEK NK+GKYWCWCFWSLEVEVLDLL AKEIAVRAWDET NTQPEKLIWNLMGMMN
Sbjct: 421 TLDHPEKANKYGKYWCWCFWSLEVEVLDLLSAKEIAVRAWDETHNTQPEKLIWNLMGMMN 480

Query: 481 NCWFRVKTNVCKPHKGEIGIIFEHPTLPGNQSGGWMDRERHLEISSESNQTLKKSVSTPF 540
           NCWFRVKTN+CKPHKGEIGI+FEHPT PGNQSGGWMDRERHLEIS+ESNQTLKKSVSTPF
Sbjct: 481 NCWFRVKTNMCKPHKGEIGIVFEHPTQPGNQSGGWMDRERHLEISTESNQTLKKSVSTPF 540

Query: 541 MNTASNTYSLSEVKKHNSPQSAWIIVHGHVYDCTRFLKDHPGGSDSILINAGTDCTEEFD 600
           MNTASNTY+LSEVKKHNSPQSAWIIVHGHVYDCTRFLKDHPGGSDSILINAGTDCTEEFD
Sbjct: 541 MNTASNTYTLSEVKKHNSPQSAWIIVHGHVYDCTRFLKDHPGGSDSILINAGTDCTEEFD 600

Query: 601 AIHSDKAKKMLEDYRIGELITTGYASDSSSNSPNNSTHGASNFSHLAPIKEITPSSPIPA 660
           AIHSDKAKKMLEDYRIGELITTGYASDSSSNSPNNSTHGASNFSHLAPI+E    +P+ +
Sbjct: 601 AIHSDKAKKMLEDYRIGELITTGYASDSSSNSPNNSTHGASNFSHLAPIRE----APV-S 660

Query: 661 RRVALAPNQKIPCKLVSKTAISHDVRVFRFALP-GQEQTLGLPVGKHIFICATVDGKLCM 720
           RRVALAPN+KIPCKL+SKT+ISHDVRVFRFALP GQ+Q LGLPVGKHIFICATVDGKLCM
Sbjct: 661 RRVALAPNEKIPCKLISKTSISHDVRVFRFALPGGQDQALGLPVGKHIFICATVDGKLCM 720

Query: 721 RAYTPTSSIDEVGFFELVVKVYFKNVHPKFPNGGVMSQYLESMEVGSTLDVKGPLGHIEY 780
           RAYTPTSSIDE+GFFELVVKVYFK VHPKFPNGG+MSQYL+SMEVGSTLDVKGPLGHIEY
Sbjct: 721 RAYTPTSSIDEMGFFELVVKVYFKGVHPKFPNGGIMSQYLDSMEVGSTLDVKGPLGHIEY 780

Query: 781 TGRGNFMVHGKPRFAKRLAMLAGGTGITPIYQVVQAILKDPEDETEMFVVYANRTEEDIL 840
           TGRGNFMVHGKPRFA+RLAMLAGGTGITPIYQVVQAILKDPEDETEM+VVYANRTE+DIL
Sbjct: 781 TGRGNFMVHGKPRFARRLAMLAGGTGITPIYQVVQAILKDPEDETEMYVVYANRTEDDIL 840

Query: 841 LREELEMWAKKNDRLKVWYVVQESIREGWEYSVGFITENILREHIPTAAEDTLALVCGPP 900
           LR+EL+ WAKKN RLKVWYVVQESIREGWEYSVGFITENILREHIP AAEDTLAL CGPP
Sbjct: 841 LRDELDTWAKKNQRLKVWYVVQESIREGWEYSVGFITENILREHIPAAAEDTLALACGPP 900

Query: 901 PMIQFAVQPNLEKMNYDTKNSMLVF 920
            MIQFAVQPNLEKMNYDTKNS+LVF
Sbjct: 901 AMIQFAVQPNLEKMNYDTKNSLLVF 918

BLAST of Spg008806 vs. ExPASy Swiss-Prot
Match: P27783 (Nitrate reductase [NAD(P)H] OS=Betula pendula OX=3505 GN=NIA1 PE=2 SV=1)

HSP 1 Score: 1543.9 bits (3996), Expect = 0.0e+00
Identity = 724/920 (78.70%), Postives = 816/920 (88.70%), Query Frame = 0

Query: 1   MAASVDNRQFGPLQPPLNGVVRTFKSGPN-HRSDSPVRGCNFPNSNIDYSNGNRSVKSSV 60
           MAASV+NR+F   +P +NG+VRTFK  PN HRSDSP  G   P+S               
Sbjct: 1   MAASVENRRFTHHEPAVNGLVRTFKPVPNSHRSDSPDLGRQIPSS------------PKK 60

Query: 61  KIEEEEDSSSDDENEINEFRELIKKGNRELEPSIVDPRDEGTADNWIERNSSMVRLTGKH 120
           ++   EDSSS+DENE N+++ELI+KGN ELEPSI+DPRDE TADNW+ERN++MVRLTGKH
Sbjct: 61  QVATGEDSSSEDENE-NDYKELIQKGNGELEPSILDPRDEATADNWVERNATMVRLTGKH 120

Query: 121 PFNSEPPLNRLMHHGFITPVPLHYVRNHGAVPKAKWSDWSIEICGLVKRPAKFTMDQLVN 180
           PFNSE PL RLMHHGFITP PLHYVRNHG VPKA+W DWS+E+CGLVKRPA+FTMD+LV 
Sbjct: 121 PFNSEAPLTRLMHHGFITPAPLHYVRNHGPVPKARWEDWSVEVCGLVKRPARFTMDRLVT 180

Query: 181 EFRFREFPATLVCAGNRRKEQNMVKKTIGFNWGAAGVSTSVWRGVPLRDVLKRCGIFSRK 240
           EFR REFP TLVCAGNRRKEQNMVKKTIGFNWGAAGVSTSVWRGVPLRDVLKRCGIFSR 
Sbjct: 181 EFRSREFPVTLVCAGNRRKEQNMVKKTIGFNWGAAGVSTSVWRGVPLRDVLKRCGIFSRG 240

Query: 241 KGALNVCFEGAEDLPGGGGSKYGTSIKKELAMDPARDIILAYMQNGEQLSPDHGFPVRMI 300
           +GA NVCFEGAEDLPGGGGSKYGTS+K E+AMDPARDIIL YMQNGE+LSPDHGFPVRMI
Sbjct: 241 RGAFNVCFEGAEDLPGGGGSKYGTSVKYEMAMDPARDIILGYMQNGERLSPDHGFPVRMI 300

Query: 301 IPGFIGGRMVKWLKRIIVTTKESENYYHYKDNRVLPSHVDAELANSEAWWYKPEYIINEL 360
           IPGFIGGRMVKWLKRIIVTTKES+NYYHY DNRVLPSHVDA++A +EAWWYKPE+IINEL
Sbjct: 301 IPGFIGGRMVKWLKRIIVTTKESDNYYHYNDNRVLPSHVDADVAKAEAWWYKPEHIINEL 360

Query: 361 NINSVITTPCHEEILPINSWTTQRPYTLRGYSYSGGGKKVTRVEVTMDSGETWHVCTLDH 420
           NINSVITTPCHEEILPINSWTTQRPYTLRGY+YSGGG+KVTRVE+TM+ GE W VC LDH
Sbjct: 361 NINSVITTPCHEEILPINSWTTQRPYTLRGYAYSGGGRKVTRVEITMNGGEKWRVCALDH 420

Query: 421 PEKPNKFGKYWCWCFWSLEVEVLDLLGAKEIAVRAWDETLNTQPEKLIWNLMGMMNNCWF 480
           PEKPNK+GKYWCWCFWSLEVEVLDLLGAKEIAVRAWDE  NTQPEKLIWN+MGMMNNCWF
Sbjct: 421 PEKPNKYGKYWCWCFWSLEVEVLDLLGAKEIAVRAWDEAHNTQPEKLIWNVMGMMNNCWF 480

Query: 481 RVKTNVCKPHKGEIGIIFEHPTLPGNQSGGWMDRERHLEISSESNQTLKKSVSTPFMNTA 540
           RVKTNVCK H GEIGI FEHPT+PGN+SGGWM RE++LE SS++NQ+LKKSVS+PFMNT+
Sbjct: 481 RVKTNVCKAHMGEIGIAFEHPTVPGNESGGWMAREKNLETSSDANQSLKKSVSSPFMNTS 540

Query: 541 SNTYSLSEVKKHNSPQSAWIIVHGHVYDCTRFLKDHPGGSDSILINAGTDCTEEFDAIHS 600
           S  +S+SEVKKHNS +SAWIIVHGH+YDCT FLKDHPGG+DSILINAGTDCTEEFDAIHS
Sbjct: 541 SKMFSMSEVKKHNSAESAWIIVHGHIYDCTHFLKDHPGGADSILINAGTDCTEEFDAIHS 600

Query: 601 DKAKKMLEDYRIGELITTGYASDSSSNSPNNSTHGASNFSHLAPIKEITPSSPIPARRVA 660
           DKAKKMLEDYRIGELITTGY SD    SPN++ HGASN SHLAPIKEI      P R VA
Sbjct: 601 DKAKKMLEDYRIGELITTGYVSD----SPNSTVHGASNTSHLAPIKEIA-----PLRNVA 660

Query: 661 LAPNQKIPCKLVSKTAISHDVRVFRFALPGQEQTLGLPVGKHIFICATVDGKLCMRAYTP 720
           L P  KIP KLV K ++SHDVR+FR ALP  +Q LGLPVGKH+F+CAT+D KLCMRAYTP
Sbjct: 661 LIPGAKIPTKLVYKKSLSHDVRLFRLALPSDDQVLGLPVGKHVFLCATIDDKLCMRAYTP 720

Query: 721 TSSIDEVGFFELVVKVYFKNVHPKFPNGGVMSQYLESMEVGSTLDVKGPLGHIEYTGRGN 780
           TS+IDEVG+ +LVVK+YFKN +P+FPNGG+MSQ+L+S+ +GS L VKGPLGH+EYTGRGN
Sbjct: 721 TSTIDEVGYLDLVVKIYFKNSNPRFPNGGLMSQHLDSLPIGSVLHVKGPLGHVEYTGRGN 780

Query: 781 FMVHGKPRFAKRLAMLAGGTGITPIYQVVQAILKDPEDETEMFVVYANRTEEDILLREEL 840
           F+VHG+P+FAKRLAM+AGGTGITPIYQV+QAILKDPEDETEMFVVYANRTE+DILLREEL
Sbjct: 781 FLVHGEPKFAKRLAMVAGGTGITPIYQVIQAILKDPEDETEMFVVYANRTEDDILLREEL 840

Query: 841 EMWAKKNDRLKVWYVVQESIREGWEYSVGFITENILREHIPTAAEDTLALVCGPPPMIQF 900
           + WAKK+++LKVWYVV+ES REGWEYSVG+I E+ILREHIP  ++D LAL CG P MI+ 
Sbjct: 841 DDWAKKHEKLKVWYVVKESKREGWEYSVGYIRESILREHIPEGSDDVLALACGAPSMIEE 898

Query: 901 AVQPNLEKMNYDTKNSMLVF 920
           AV+ NLEKMNYDTKNS+++F
Sbjct: 901 AVRLNLEKMNYDTKNSLIIF 898

BLAST of Spg008806 vs. ExPASy Swiss-Prot
Match: P36859 (Nitrate reductase [NADH] OS=Petunia hybrida OX=4102 GN=NIA PE=2 SV=1)

HSP 1 Score: 1539.2 bits (3984), Expect = 0.0e+00
Identity = 724/921 (78.61%), Postives = 817/921 (88.71%), Query Frame = 0

Query: 1   MAASVDNRQFGPLQPPLNGVVRTFKSGPNHRSDSPVRGCNFPNSNIDYSNGNRSVKSSVK 60
           MAASV+NRQF  L+P L+GVVR+FK     RSDSPVRGCNFP +N + +N  +   +++ 
Sbjct: 1   MAASVENRQFSHLEPGLSGVVRSFKP----RSDSPVRGCNFPLNN-ELTNFQKKPNTTIY 60

Query: 61  IEEEEDSSSDDENEINEFRELIKKGNRELEPSIVDPRDEGTADNWIERNSSMVRLTGKHP 120
           ++       DD+++ NE+ ++I+KG  E+EPS+ D RDEGTADNWIERN+SM+RLTGKHP
Sbjct: 61  LDCSSSEDDDDDDDKNEYLQMIRKGKLEVEPSVHDIRDEGTADNWIERNNSMIRLTGKHP 120

Query: 121 FNSEPPLNRLMHHGFITPVPLHYVRNHGAVPKAKWSDWSIEICGLVKRPAKFTMDQLVNE 180
           FNSEPPL RLMHHGFITPVPLHYVRNHG VPK  W DW++E+ GLVKRP KFTM+QLVNE
Sbjct: 121 FNSEPPLARLMHHGFITPVPLHYVRNHGPVPKGMWDDWTVEVTGLVKRPMKFTMEQLVNE 180

Query: 181 FRFREFPATLVCAGNRRKEQNMVKKTIGFNWGAAGVSTSVWRGVPLRDVLKRCGIFSRKK 240
           F  RE P TLVCAGNRRKEQNMVK+TIGFNWGAA VST+VWRGVPLR +LKRCGI+SR K
Sbjct: 181 FPSRELPVTLVCAGNRRKEQNMVKQTIGFNWGAAAVSTTVWRGVPLRAILKRCGIYSRTK 240

Query: 241 GALNVCFEGAEDLPGGGGSKYGTSIKKELAMDPARDIILAYMQNGEQLSPDHGFPVRMII 300
           GALN+CFEGA+ LPGGGGSKYGTSIKKE AMDP+RDII+AYMQNGE+L+PDHGFP+RMII
Sbjct: 241 GALNICFEGADVLPGGGGSKYGTSIKKEFAMDPSRDIIIAYMQNGEKLTPDHGFPLRMII 300

Query: 301 PGFIGGRMVKWLKRIIVTTKESENYYHYKDNRVLPSHVDAELANSEAWWYKPEYIINELN 360
           PGFIGGRMVKWLKRIIVTT+ESE+YYHYKDNRVLP HVDAELAN+EAWWYKPEYIINELN
Sbjct: 301 PGFIGGRMVKWLKRIIVTTQESESYYHYKDNRVLPPHVDAELANAEAWWYKPEYIINELN 360

Query: 361 INSVITTPCHEEILPINSWTTQRPYTLRGYSYSGGGKKVTRVEVTMDSGETWHVCTLDHP 420
           INSVITTPCHEEILPINSWTTQRPYTLRGYSYSGGGKKVTRVEVTMD GETW+VCT+DHP
Sbjct: 361 INSVITTPCHEEILPINSWTTQRPYTLRGYSYSGGGKKVTRVEVTMDGGETWNVCTVDHP 420

Query: 421 EKPNKFGKYWCWCFWSLEVEVLDLLGAKEIAVRAWDETLNTQPEKLIWNLMGMMNNCWFR 480
           EKPNK+GKYWCWCFWSLEVEVLDLL AKEIAVRAWDETLNTQPEKLIWN+MGMMNNCWFR
Sbjct: 421 EKPNKYGKYWCWCFWSLEVEVLDLLSAKEIAVRAWDETLNTQPEKLIWNVMGMMNNCWFR 480

Query: 481 VKTNVCKPHKGEIGIIFEHPTLPGNQSGGWMDRERHLEISSESNQTLKKSVSTPFMNTAS 540
           VKTNVCKPHKGEIGI+FEHPT PGN SGGWM +ERHLEIS+E+  TLKKS+STPFMNTAS
Sbjct: 481 VKTNVCKPHKGEIGIVFEHPTQPGNLSGGWMAKERHLEISAEAPPTLKKSISTPFMNTAS 540

Query: 541 NTYSLSEVKKHNSPQSAWIIVHGHVYDCTRFLKDHPGGSDSILINAGTDCTEEFDAIHSD 600
             YS+SEVKKHNS  SAWIIVHGHVYD TRFLKDHPGG DSILINAGTDCTEEFDAIHSD
Sbjct: 541 KMYSMSEVKKHNSADSAWIIVHGHVYDATRFLKDHPGGIDSILINAGTDCTEEFDAIHSD 600

Query: 601 KAKKMLEDYRIGELITTGYASDSSSNSPNNSTHGASNFS-HLAPIKEITPSSPIPARRVA 660
           KAKK+LED+RIGELITTGY SDS   SPNNS HG+S+FS  LAPIKE+ P+     R VA
Sbjct: 601 KAKKLLEDFRIGELITTGYTSDS---SPNNSVHGSSSFSGFLAPIKELAPA----VRSVA 660

Query: 661 LAPNQKIPCKLVSKTAISHDVRVFRFALPGQEQTLGLPVGKHIFICATVDGKLCMRAYTP 720
           L P +KIPCKLV K +ISHDVR FRFALP ++Q LGLPVGKHIF+CA +D KLCMRAYTP
Sbjct: 661 LIPREKIPCKLVDKKSISHDVRKFRFALPSEDQVLGLPVGKHIFLCAIIDDKLCMRAYTP 720

Query: 721 TSSIDEVGFFELVVKVYFKNVHPKFPNGGVMSQYLESMEVGSTLDVKGPLGHIEYTGRGN 780
           TS++DEVG+FELVVK+YFK + PKFPNGG MSQYL+S+ +G+ +DVKGPLGHIEY GRGN
Sbjct: 721 TSTVDEVGYFELVVKIYFKGIVPKFPNGGQMSQYLDSLPLGAFVDVKGPLGHIEYQGRGN 780

Query: 781 FMVHGKPRFAKRLAMLAGGTGITPIYQVVQAILKDPEDETEMFVVYANRTEEDILLREEL 840
           F+VHGK +FAK+LAMLAGGTGITP+YQV+QAILKDPEDETEM VVYANRTE+DILL++EL
Sbjct: 781 FLVHGKRKFAKKLAMLAGGTGITPVYQVMQAILKDPEDETEMHVVYANRTEDDILLKDEL 840

Query: 841 EMWA-KKNDRLKVWYVVQESIREGWEYSVGFITENILREHIPTAAEDTLALVCGPPPMIQ 900
           + WA K  +R+KVWYVVQ+SI+EGW+YS GFITE +LREHIP  ++ TLAL CGPPPMIQ
Sbjct: 841 DSWAVKLPERVKVWYVVQDSIKEGWKYSTGFITEAVLREHIPLPSQTTLALACGPPPMIQ 900

Query: 901 FAVQPNLEKMNYDTKNSMLVF 920
           FAV PNLEKM YD K+S+LVF
Sbjct: 901 FAVNPNLEKMGYDIKDSLLVF 909

BLAST of Spg008806 vs. ExPASy Swiss-Prot
Match: P08509 (Nitrate reductase [NADH] 2 OS=Nicotiana tabacum OX=4097 GN=NIA2 PE=2 SV=2)

HSP 1 Score: 1534.6 bits (3972), Expect = 0.0e+00
Identity = 721/921 (78.28%), Postives = 813/921 (88.27%), Query Frame = 0

Query: 1   MAASVDNRQFGPLQPPLNGVVRTFKSGPNHRSDSPVRGCNFPNSNIDYSNGNRSVKSSVK 60
           MAASV+NRQF  L+    G+ R+FK     RSDSPVRGCNFP+ N   +N  +   S++ 
Sbjct: 1   MAASVENRQFSHLEA---GLSRSFKP----RSDSPVRGCNFPSPN--STNFQKKPNSTIY 60

Query: 61  IEEEEDSSSDDENEINEFRELIKKGNRELEPSIVDPRDEGTADNWIERNSSMVRLTGKHP 120
           ++       DD++E NE+ ++IKKGN ELEPS+ D RDEGTADNWIERN SM+RLTGKHP
Sbjct: 61  LDYSSSEDDDDDDEKNEYLQMIKKGNSELEPSVHDTRDEGTADNWIERNFSMIRLTGKHP 120

Query: 121 FNSEPPLNRLMHHGFITPVPLHYVRNHGAVPKAKWSDWSIEICGLVKRPAKFTMDQLVNE 180
           FNSEPPLNRLMHHGFITPVPLHYVRNHG VPK  W DW++E+ GLVKRP KFTMDQLVNE
Sbjct: 121 FNSEPPLNRLMHHGFITPVPLHYVRNHGPVPKGTWDDWTVEVTGLVKRPMKFTMDQLVNE 180

Query: 181 FRFREFPATLVCAGNRRKEQNMVKKTIGFNWGAAGVSTSVWRGVPLRDVLKRCGIFSRKK 240
           F  RE P TLVCAGNRRKEQNMVK+TIGFNWGAA VST++WRGVPLR +LKRCG+FS+ K
Sbjct: 181 FPCRELPVTLVCAGNRRKEQNMVKQTIGFNWGAAAVSTTIWRGVPLRALLKRCGVFSKNK 240

Query: 241 GALNVCFEGAEDLPGGGGSKYGTSIKKELAMDPARDIILAYMQNGEQLSPDHGFPVRMII 300
           GALNVCFEGA+ LPGGGGSKYGTSIKKE AMDPARDII+AYMQNGE+L+PDHGFPVRMII
Sbjct: 241 GALNVCFEGADVLPGGGGSKYGTSIKKEFAMDPARDIIVAYMQNGEKLAPDHGFPVRMII 300

Query: 301 PGFIGGRMVKWLKRIIVTTKESENYYHYKDNRVLPSHVDAELANSEAWWYKPEYIINELN 360
           PGFIGGRMVKW+KRIIVTT+ES++YYH+KDNRVLP HVDAELAN+EAWWYKPEYIINELN
Sbjct: 301 PGFIGGRMVKWIKRIIVTTQESDSYYHFKDNRVLPPHVDAELANTEAWWYKPEYIINELN 360

Query: 361 INSVITTPCHEEILPINSWTTQRPYTLRGYSYSGGGKKVTRVEVTMDSGETWHVCTLDHP 420
           INSVITTPCHEEILPIN+WTTQRPYTLRGYSYSGGGKKVTRVEVT+D GETW V TLDHP
Sbjct: 361 INSVITTPCHEEILPINAWTTQRPYTLRGYSYSGGGKKVTRVEVTLDGGETWQVSTLDHP 420

Query: 421 EKPNKFGKYWCWCFWSLEVEVLDLLGAKEIAVRAWDETLNTQPEKLIWNLMGMMNNCWFR 480
           EKP K+GKYWCWCFWSLEVEVLDLL AKEIAVRAWDETLNTQPEKLIWN+MGMMNNCWFR
Sbjct: 421 EKPTKYGKYWCWCFWSLEVEVLDLLSAKEIAVRAWDETLNTQPEKLIWNVMGMMNNCWFR 480

Query: 481 VKTNVCKPHKGEIGIIFEHPTLPGNQSGGWMDRERHLEISSESNQTLKKSVSTPFMNTAS 540
           VK NVCKPHKGEIGI+FEHPT PGNQSGGWM +ERHLEIS+E+ QTLKKS+STPFMNTAS
Sbjct: 481 VKMNVCKPHKGEIGIVFEHPTQPGNQSGGWMAKERHLEISAEAPQTLKKSISTPFMNTAS 540

Query: 541 NTYSLSEVKKHNSPQSAWIIVHGHVYDCTRFLKDHPGGSDSILINAGTDCTEEFDAIHSD 600
             YS+SEV+KH+S  SAWIIVHGH+YD TRFLKDHPGG+DSILINAGTDCTEEFDAIHSD
Sbjct: 541 KMYSMSEVRKHSSADSAWIIVHGHIYDATRFLKDHPGGTDSILINAGTDCTEEFDAIHSD 600

Query: 601 KAKKMLEDYRIGELITTGYASDSSSNSPNNSTHGASNF-SHLAPIKEITPSSPIPARRVA 660
           KAKK+LED+RIGELITTGY SD    SP NS HG+S+F S LAPIKE+ P+     R VA
Sbjct: 601 KAKKLLEDFRIGELITTGYTSD----SPGNSVHGSSSFSSFLAPIKELVPAQ----RSVA 660

Query: 661 LAPNQKIPCKLVSKTAISHDVRVFRFALPGQEQTLGLPVGKHIFICATVDGKLCMRAYTP 720
           L P +KIPCKL+ K +ISHDVR FRFALP ++Q LGLPVGKHIF+CA +D KLCMRAYTP
Sbjct: 661 LIPREKIPCKLIDKQSISHDVRKFRFALPSEDQVLGLPVGKHIFLCAVIDDKLCMRAYTP 720

Query: 721 TSSIDEVGFFELVVKVYFKNVHPKFPNGGVMSQYLESMEVGSTLDVKGPLGHIEYTGRGN 780
           TS+IDEVG+FELVVK+YFK +HPKFPNGG MSQYL+SM +GS LDVKGPLGHIEY G+GN
Sbjct: 721 TSTIDEVGYFELVVKIYFKGIHPKFPNGGQMSQYLDSMPLGSFLDVKGPLGHIEYQGKGN 780

Query: 781 FMVHGKPRFAKRLAMLAGGTGITPIYQVVQAILKDPEDETEMFVVYANRTEEDILLREEL 840
           F+VHGK +FAK+LAM+AGGTGITP+YQV+QAILKDPED+TEM+VVYANRTE+DILL+EEL
Sbjct: 781 FLVHGKQKFAKKLAMIAGGTGITPVYQVMQAILKDPEDDTEMYVVYANRTEDDILLKEEL 840

Query: 841 EMWAKK-NDRLKVWYVVQESIREGWEYSVGFITENILREHIPTAAEDTLALVCGPPPMIQ 900
           + WA+K  +R+KVWYVVQ+SI+EGW+YS+GFITE ILREHIP  +  TLAL CGPPPMIQ
Sbjct: 841 DSWAEKIPERVKVWYVVQDSIKEGWKYSIGFITEAILREHIPEPSHTTLALACGPPPMIQ 900

Query: 901 FAVQPNLEKMNYDTKNSMLVF 920
           FAV PNLEKM YD K+S+LVF
Sbjct: 901 FAVNPNLEKMGYDIKDSLLVF 904

BLAST of Spg008806 vs. ExPASy Swiss-Prot
Match: P11605 (Nitrate reductase [NADH] 1 OS=Nicotiana tabacum OX=4097 GN=NIA1 PE=3 SV=1)

HSP 1 Score: 1521.9 bits (3939), Expect = 0.0e+00
Identity = 715/921 (77.63%), Postives = 811/921 (88.06%), Query Frame = 0

Query: 1   MAASVDNRQFGPLQPPLNGVVRTFKSGPNHRSDSPVRGCNFPNSNIDYSNGNRSVKSSVK 60
           MAASV+NRQF  ++    G+ R+FK     RSDSPVRGCNFP  N   +N  +   S++ 
Sbjct: 1   MAASVENRQFSHIEA---GLSRSFKP----RSDSPVRGCNFPPPN--STNFQKKPNSTIF 60

Query: 61  IEEEEDSSSDDENEINEFRELIKKGNRELEPSIVDPRDEGTADNWIERNSSMVRLTGKHP 120
           ++       DD++E NE+ ++IKKGN ELEPS+ D RDEGTADNWIERN S++RLTGKHP
Sbjct: 61  LDYSSSEDDDDDDEKNEYLQMIKKGNSELEPSVHDSRDEGTADNWIERNFSLIRLTGKHP 120

Query: 121 FNSEPPLNRLMHHGFITPVPLHYVRNHGAVPKAKWSDWSIEICGLVKRPAKFTMDQLVNE 180
           FNSEPPLNRLMHHGFITPVPLHYVRNHG VPK  W DW++E+ GLVKRP KFTMDQLVNE
Sbjct: 121 FNSEPPLNRLMHHGFITPVPLHYVRNHGPVPKGTWDDWTVEVTGLVKRPMKFTMDQLVNE 180

Query: 181 FRFREFPATLVCAGNRRKEQNMVKKTIGFNWGAAGVSTSVWRGVPLRDVLKRCGIFSRKK 240
           F  RE P TLVCAGNRRKEQNMVK+TIGFNWGAA VST+VWRGVPLR +LKR G+FS+ K
Sbjct: 181 FPSRELPVTLVCAGNRRKEQNMVKQTIGFNWGAAAVSTTVWRGVPLRALLKRYGVFSKNK 240

Query: 241 GALNVCFEGAEDLPGGGGSKYGTSIKKELAMDPARDIILAYMQNGEQLSPDHGFPVRMII 300
           GALNVCFEGA+ LPGGGGSKYGTSIKKE AMDPARDII+AYMQNGE+L+PDHGFPVRMII
Sbjct: 241 GALNVCFEGADVLPGGGGSKYGTSIKKEFAMDPARDIIIAYMQNGEKLAPDHGFPVRMII 300

Query: 301 PGFIGGRMVKWLKRIIVTTKESENYYHYKDNRVLPSHVDAELANSEAWWYKPEYIINELN 360
           PGFIGGRMVKW+KRIIVTT+ES++YYH+KDNRVLP HVDAELAN+EAWWYKPEYIINELN
Sbjct: 301 PGFIGGRMVKWIKRIIVTTQESDSYYHFKDNRVLPPHVDAELANTEAWWYKPEYIINELN 360

Query: 361 INSVITTPCHEEILPINSWTTQRPYTLRGYSYSGGGKKVTRVEVTMDSGETWHVCTLDHP 420
           INSVITTPCHEEILPIN+WTTQRPYTLRGYSYSGGGKKVTRVEVT+D GETW VCTLDHP
Sbjct: 361 INSVITTPCHEEILPINAWTTQRPYTLRGYSYSGGGKKVTRVEVTLDGGETWQVCTLDHP 420

Query: 421 EKPNKFGKYWCWCFWSLEVEVLDLLGAKEIAVRAWDETLNTQPEKLIWNLMGMMNNCWFR 480
           EKP K+GKYWCWCFWSLEVEVLDLL AKEIAVRAWDETLNTQPEKLIWN+MGMMNNCWFR
Sbjct: 421 EKPTKYGKYWCWCFWSLEVEVLDLLSAKEIAVRAWDETLNTQPEKLIWNVMGMMNNCWFR 480

Query: 481 VKTNVCKPHKGEIGIIFEHPTLPGNQSGGWMDRERHLEISSESNQTLKKSVSTPFMNTAS 540
           VK NVCKPHKGEIGI+FEHPT PGNQSGGWM +ERHLEIS+E+  TLKKS+STPFMNTAS
Sbjct: 481 VKMNVCKPHKGEIGIVFEHPTQPGNQSGGWMAKERHLEISAEAPPTLKKSISTPFMNTAS 540

Query: 541 NTYSLSEVKKHNSPQSAWIIVHGHVYDCTRFLKDHPGGSDSILINAGTDCTEEFDAIHSD 600
             YS+SEV+KH+S  SAWIIVHGH+YD TRFLKDHPGGSDSILINAGTDCTEEFDAIHSD
Sbjct: 541 KMYSMSEVRKHSSADSAWIIVHGHIYDATRFLKDHPGGSDSILINAGTDCTEEFDAIHSD 600

Query: 601 KAKKMLEDYRIGELITTGYASDSSSNSPNNSTHGASNF-SHLAPIKEITPSSPIPARRVA 660
           KAKK+LE++RIGEL+TTGY SD    SP NS HG+S+F S LAPIKE+ P+     R VA
Sbjct: 601 KAKKLLEEFRIGELLTTGYTSD----SPGNSVHGSSSFSSFLAPIKELVPAQ----RSVA 660

Query: 661 LAPNQKIPCKLVSKTAISHDVRVFRFALPGQEQTLGLPVGKHIFICATVDGKLCMRAYTP 720
           L P +KIPCKL+ K +IS DVR FRFALP ++Q LGLPVGKHIF+CA +D KLCMRAYTP
Sbjct: 661 LIPREKIPCKLIDKQSISPDVRKFRFALPSEDQVLGLPVGKHIFLCAVIDDKLCMRAYTP 720

Query: 721 TSSIDEVGFFELVVKVYFKNVHPKFPNGGVMSQYLESMEVGSTLDVKGPLGHIEYTGRGN 780
           TS+IDEVG+FELVVK+YFK +HPKFPNGG MSQYL+S+++GS LDVKGPLGHIEY G+GN
Sbjct: 721 TSTIDEVGYFELVVKIYFKGIHPKFPNGGQMSQYLDSLQLGSFLDVKGPLGHIEYQGKGN 780

Query: 781 FMVHGKPRFAKRLAMLAGGTGITPIYQVVQAILKDPEDETEMFVVYANRTEEDILLREEL 840
           F+VHGK +FAK+LAM+AGGTGITP+YQV+QAILKDPED+TEM+VVYANRTE+DILL+EEL
Sbjct: 781 FLVHGKQKFAKKLAMIAGGTGITPVYQVMQAILKDPEDDTEMYVVYANRTEDDILLKEEL 840

Query: 841 EMWAKK-NDRLKVWYVVQESIREGWEYSVGFITENILREHIPTAAEDTLALVCGPPPMIQ 900
           + WA+K  +R+KVWYVVQ+SI+EGW+YS+GFI+E ILREHIP  +  TLAL CGPPPMIQ
Sbjct: 841 DSWAEKIPERVKVWYVVQDSIKEGWKYSLGFISEAILREHIPEPSHTTLALACGPPPMIQ 900

Query: 901 FAVQPNLEKMNYDTKNSMLVF 920
           FAV PNLEKM YD K+S+LVF
Sbjct: 901 FAVNPNLEKMGYDIKDSLLVF 904

BLAST of Spg008806 vs. ExPASy TrEMBL
Match: E2IZV5 (Nitrate reductase OS=Cucumis sativus OX=3659 GN=NR2 PE=2 SV=1)

HSP 1 Score: 1780.0 bits (4609), Expect = 0.0e+00
Identity = 845/923 (91.55%), Postives = 888/923 (96.21%), Query Frame = 0

Query: 1   MAASVDNRQFGPLQPPLNGVVRTFKSGPNHRSDSPVRGCNFPNSNIDYSNGNRSVKSSVK 60
           MAASVDNRQFG LQPPLNGVVR+FKSGPNHRSDSPVRGCNFPNSNI+    NR +KSSVK
Sbjct: 1   MAASVDNRQFGSLQPPLNGVVRSFKSGPNHRSDSPVRGCNFPNSNIN----NRLLKSSVK 60

Query: 61  IEEEE---DSSSDDENEINEFRELIKKGNRELEPSIVDPRDEGTADNWIERNSSMVRLTG 120
           +E+EE   DS S+DEN+ NE RELIKKGNRELEPS VDPRDEGTADNWIERNSSMVRLTG
Sbjct: 61  MEQEEEDDDSCSEDENDNNELRELIKKGNRELEPSTVDPRDEGTADNWIERNSSMVRLTG 120

Query: 121 KHPFNSEPPLNRLMHHGFITPVPLHYVRNHGAVPKAKWSDWSIEICGLVKRPAKFTMDQL 180
           KHPFNSEPPLNRLMHHGFITPVPLHYVRNHGAVPKAKWSDW+IE+CGLVKRP KF+MDQL
Sbjct: 121 KHPFNSEPPLNRLMHHGFITPVPLHYVRNHGAVPKAKWSDWTIEVCGLVKRPTKFSMDQL 180

Query: 181 VNEFRFREFPATLVCAGNRRKEQNMVKKTIGFNWGAAGVSTSVWRGVPLRDVLKRCGIFS 240
           VNEFRFREFPATLVCAGNRRKEQNMVKK+IGFNWGAAGVSTSVWRGVPLR+VLKRCGI S
Sbjct: 181 VNEFRFREFPATLVCAGNRRKEQNMVKKSIGFNWGAAGVSTSVWRGVPLREVLKRCGIMS 240

Query: 241 RKKGALNVCFEGAEDLPGGGGSKYGTSIKKELAMDPARDIILAYMQNGEQLSPDHGFPVR 300
           RKKGALNVCFEGAEDLPGGGGSKYGTSIKKELAMDPARDIILAYMQNGEQL+PDHGFPVR
Sbjct: 241 RKKGALNVCFEGAEDLPGGGGSKYGTSIKKELAMDPARDIILAYMQNGEQLAPDHGFPVR 300

Query: 301 MIIPGFIGGRMVKWLKRIIVTTKESENYYHYKDNRVLPSHVDAELANSEAWWYKPEYIIN 360
           MIIPGFIGGRMVKWLKRIIVTTKESENYYH+KDNRVLPSHVDAELAN+EAWWYKPEYIIN
Sbjct: 301 MIIPGFIGGRMVKWLKRIIVTTKESENYYHFKDNRVLPSHVDAELANAEAWWYKPEYIIN 360

Query: 361 ELNINSVITTPCHEEILPINSWTTQRPYTLRGYSYSGGGKKVTRVEVTMDSGETWHVCTL 420
           ELNINSVITTPCHEEILPINSWTTQRPYTLRGYSYSGGGKKVTRVEVTMD GETW VCTL
Sbjct: 361 ELNINSVITTPCHEEILPINSWTTQRPYTLRGYSYSGGGKKVTRVEVTMDGGETWQVCTL 420

Query: 421 DHPEKPNKFGKYWCWCFWSLEVEVLDLLGAKEIAVRAWDETLNTQPEKLIWNLMGMMNNC 480
           DHPEKPNK+GKYWCWCFWSLEVEVLDLLGAKEIAVRAWDETLNTQPEKLIWNLMGMMNNC
Sbjct: 421 DHPEKPNKYGKYWCWCFWSLEVEVLDLLGAKEIAVRAWDETLNTQPEKLIWNLMGMMNNC 480

Query: 481 WFRVKTNVCKPHKGEIGIIFEHPTLPGNQSGGWMDRERHLEISSESNQTLKKSVSTPFMN 540
           WF VKTNVCKPHKGEIGIIFEHPT+PGNQSGGWMDRERHLEIS+ESNQTLKKS+STPFMN
Sbjct: 481 WFTVKTNVCKPHKGEIGIIFEHPTVPGNQSGGWMDRERHLEISTESNQTLKKSISTPFMN 540

Query: 541 TASNTYSLSEVKKHNSPQSAWIIVHGHVYDCTRFLKDHPGGSDSILINAGTDCTEEFDAI 600
           TASNTYSLSEVKKHNSPQSAWIIVHGHVYDCTRFLKDHPGGSDSILINAGTDCTEEFDAI
Sbjct: 541 TASNTYSLSEVKKHNSPQSAWIIVHGHVYDCTRFLKDHPGGSDSILINAGTDCTEEFDAI 600

Query: 601 HSDKAKKMLEDYRIGELITTGYASDSSSNSPNNSTHGASNFSHLAPIKEITPSSPIPARR 660
           HSDKAKKMLEDYRIGELITTGYASDSSSNSPNNSTHGASNFSHLAPI+E    +P+  RR
Sbjct: 601 HSDKAKKMLEDYRIGELITTGYASDSSSNSPNNSTHGASNFSHLAPIRE----APMVTRR 660

Query: 661 VALAPNQKIPCKLVSKTAISHDVRVFRFALP-GQEQTLGLPVGKHIFICATVDGKLCMRA 720
            ALAPN+KIPCKL+SKT ISHDVRVFRF LP GQ+Q LGLPVGKHIFICA VDGKLCMRA
Sbjct: 661 AALAPNEKIPCKLISKTEISHDVRVFRFELPGGQDQVLGLPVGKHIFICAKVDGKLCMRA 720

Query: 721 YTPTSSIDEVGFFELVVKVYFKNVHPKFPNGGVMSQYLESMEVGSTLDVKGPLGHIEYTG 780
           YTP+S++D++G+FELVVKVYFKNVHPKFPNGG+MSQ+L++MEVGST++VKGPLGHIEYTG
Sbjct: 721 YTPSSTVDQMGYFELVVKVYFKNVHPKFPNGGIMSQFLDNMEVGSTVEVKGPLGHIEYTG 780

Query: 781 RGNFMVHGKPRFAKRLAMLAGGTGITPIYQVVQAILKDPEDETEMFVVYANRTEEDILLR 840
           RGNF VHGKPRFAKRLAMLAGGTGITPIYQ+VQAILKDPEDETEMFVVYANRTE+DILLR
Sbjct: 781 RGNFTVHGKPRFAKRLAMLAGGTGITPIYQIVQAILKDPEDETEMFVVYANRTEDDILLR 840

Query: 841 EELEMWAKKNDRLKVWYVVQESIREGWEYSVGFITENILREHIPTAAEDTLALVCGPPPM 900
           EEL+ WAKKN+RLKVWYVVQESIREGWEYS+GFITE I REH+P AAEDTLALVCGPPPM
Sbjct: 841 EELDTWAKKNERLKVWYVVQESIREGWEYSIGFITEEITREHLPAAAEDTLALVCGPPPM 900

Query: 901 IQFAVQPNLEKMNYDTKNSMLVF 920
           IQFAVQPNLEKMNYDTKNSMLVF
Sbjct: 901 IQFAVQPNLEKMNYDTKNSMLVF 915

BLAST of Spg008806 vs. ExPASy TrEMBL
Match: A0A5D3DS07 (Nitrate reductase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold313G00730 PE=3 SV=1)

HSP 1 Score: 1777.3 bits (4602), Expect = 0.0e+00
Identity = 845/923 (91.55%), Postives = 887/923 (96.10%), Query Frame = 0

Query: 1   MAASVDNRQFGPLQPPLNGVVRTFKSGPNHRSDSPVRGCNFPNSNIDYSNGNRSVKSSVK 60
           MAASVDNRQFG LQPPLNGVVR+FKSGPN RSDSPVRGCNFPNSNI+    NR +KSSVK
Sbjct: 1   MAASVDNRQFGSLQPPLNGVVRSFKSGPNRRSDSPVRGCNFPNSNIN----NRLLKSSVK 60

Query: 61  IEEEE---DSSSDDENEINEFRELIKKGNRELEPSIVDPRDEGTADNWIERNSSMVRLTG 120
           +E+EE   DS S+DEN+ NE RELIKKGNRELEPSIVDPRDE TADNWIERNSSMVRLTG
Sbjct: 61  MEQEEEDDDSCSEDENDNNELRELIKKGNRELEPSIVDPRDEATADNWIERNSSMVRLTG 120

Query: 121 KHPFNSEPPLNRLMHHGFITPVPLHYVRNHGAVPKAKWSDWSIEICGLVKRPAKFTMDQL 180
           KHPFNSEPPLNRLMHHGFITPVPLHYVRNHGAVPKA WSDW+IE+CGLVKRP KFTMDQL
Sbjct: 121 KHPFNSEPPLNRLMHHGFITPVPLHYVRNHGAVPKANWSDWTIEVCGLVKRPTKFTMDQL 180

Query: 181 VNEFRFREFPATLVCAGNRRKEQNMVKKTIGFNWGAAGVSTSVWRGVPLRDVLKRCGIFS 240
           VNEFRFREFPATLVCAGNRRKEQNMVKK+IGFNWGAAGVSTSVWRGVPLRDVLKRCGI S
Sbjct: 181 VNEFRFREFPATLVCAGNRRKEQNMVKKSIGFNWGAAGVSTSVWRGVPLRDVLKRCGIMS 240

Query: 241 RKKGALNVCFEGAEDLPGGGGSKYGTSIKKELAMDPARDIILAYMQNGEQLSPDHGFPVR 300
           RKKGALNVCFEGAEDLPGGGGSKYGTSIKKELAMDPARDIILAYMQNGEQL PDHGFPVR
Sbjct: 241 RKKGALNVCFEGAEDLPGGGGSKYGTSIKKELAMDPARDIILAYMQNGEQLVPDHGFPVR 300

Query: 301 MIIPGFIGGRMVKWLKRIIVTTKESENYYHYKDNRVLPSHVDAELANSEAWWYKPEYIIN 360
           MIIPGFIGGRMVKWLKRIIVTTKESENYYH+KDNRVLPSHVDAELAN+EAWWYKPEYIIN
Sbjct: 301 MIIPGFIGGRMVKWLKRIIVTTKESENYYHFKDNRVLPSHVDAELANAEAWWYKPEYIIN 360

Query: 361 ELNINSVITTPCHEEILPINSWTTQRPYTLRGYSYSGGGKKVTRVEVTMDSGETWHVCTL 420
           ELNINSVITTPCHEEILPINSWTTQRPYTLRGYSYSGGGKKVTRVEVTMD GETW VCTL
Sbjct: 361 ELNINSVITTPCHEEILPINSWTTQRPYTLRGYSYSGGGKKVTRVEVTMDGGETWQVCTL 420

Query: 421 DHPEKPNKFGKYWCWCFWSLEVEVLDLLGAKEIAVRAWDETLNTQPEKLIWNLMGMMNNC 480
           DHPEKPNK+GKYWCWCFWSLEVEVLDLLGAKEIAVRAWDETLNTQPEKLIWNLMGMMNNC
Sbjct: 421 DHPEKPNKYGKYWCWCFWSLEVEVLDLLGAKEIAVRAWDETLNTQPEKLIWNLMGMMNNC 480

Query: 481 WFRVKTNVCKPHKGEIGIIFEHPTLPGNQSGGWMDRERHLEISSESNQTLKKSVSTPFMN 540
           WF VKTNVCKPHKGEIGIIFEHPT+PGNQSGGWMDRERHLEIS+ESNQTLKKS+STPFMN
Sbjct: 481 WFTVKTNVCKPHKGEIGIIFEHPTVPGNQSGGWMDRERHLEISTESNQTLKKSISTPFMN 540

Query: 541 TASNTYSLSEVKKHNSPQSAWIIVHGHVYDCTRFLKDHPGGSDSILINAGTDCTEEFDAI 600
           TASNTYSLSEVKKHNSPQSAWIIVHGHVYDCTRFLKDHPGGSDSILINAGTDCTEEFDAI
Sbjct: 541 TASNTYSLSEVKKHNSPQSAWIIVHGHVYDCTRFLKDHPGGSDSILINAGTDCTEEFDAI 600

Query: 601 HSDKAKKMLEDYRIGELITTGYASDSSSNSPNNSTHGASNFSHLAPIKEITPSSPIPARR 660
           HSDKAKKMLEDYRIGELITTGYASDSSSNSPNNSTHGASNFSHLAPI+E    +P+  RR
Sbjct: 601 HSDKAKKMLEDYRIGELITTGYASDSSSNSPNNSTHGASNFSHLAPIRE----APVVTRR 660

Query: 661 VALAPNQKIPCKLVSKTAISHDVRVFRFALP-GQEQTLGLPVGKHIFICATVDGKLCMRA 720
           VALAPN+KIPCKL+SKTAISHDVRVFRF LP GQ+Q LGLPVGKHIFICA V+GKLCMRA
Sbjct: 661 VALAPNEKIPCKLISKTAISHDVRVFRFELPGGQDQVLGLPVGKHIFICAKVEGKLCMRA 720

Query: 721 YTPTSSIDEVGFFELVVKVYFKNVHPKFPNGGVMSQYLESMEVGSTLDVKGPLGHIEYTG 780
           YTP++++D++G+FELVVKVYFKNVHPKFPNGG+MSQ+L++MEVGST++VKGPLGHIEYTG
Sbjct: 721 YTPSTTVDQMGYFELVVKVYFKNVHPKFPNGGIMSQFLDNMEVGSTVEVKGPLGHIEYTG 780

Query: 781 RGNFMVHGKPRFAKRLAMLAGGTGITPIYQVVQAILKDPEDETEMFVVYANRTEEDILLR 840
            GNF VHGKPRFAKRLAMLAGGTGITPIYQVVQAILKDPEDETEMFVVYANRTE+DILLR
Sbjct: 781 SGNFTVHGKPRFAKRLAMLAGGTGITPIYQVVQAILKDPEDETEMFVVYANRTEDDILLR 840

Query: 841 EELEMWAKKNDRLKVWYVVQESIREGWEYSVGFITENILREHIPTAAEDTLALVCGPPPM 900
           EEL+ WAKKN+RLKVWYVVQESIREGWEYS+GFITE I+REH+P AAEDTLALVCGPPPM
Sbjct: 841 EELDTWAKKNERLKVWYVVQESIREGWEYSIGFITEEIMREHLPAAAEDTLALVCGPPPM 900

Query: 901 IQFAVQPNLEKMNYDTKNSMLVF 920
           IQFAVQPNLEKMNYDTKNSMLVF
Sbjct: 901 IQFAVQPNLEKMNYDTKNSMLVF 915

BLAST of Spg008806 vs. ExPASy TrEMBL
Match: A0A5A7USP6 (Nitrate reductase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold1991G00030 PE=3 SV=1)

HSP 1 Score: 1776.1 bits (4599), Expect = 0.0e+00
Identity = 844/923 (91.44%), Postives = 887/923 (96.10%), Query Frame = 0

Query: 1   MAASVDNRQFGPLQPPLNGVVRTFKSGPNHRSDSPVRGCNFPNSNIDYSNGNRSVKSSVK 60
           MAASVDNRQFG LQPPLNGVVR+FKSGPN RSDSPVRGCNFPNSNI+    NR +KSSVK
Sbjct: 1   MAASVDNRQFGSLQPPLNGVVRSFKSGPNRRSDSPVRGCNFPNSNIN----NRLLKSSVK 60

Query: 61  IEEEE---DSSSDDENEINEFRELIKKGNRELEPSIVDPRDEGTADNWIERNSSMVRLTG 120
           +E+EE   DS S+DEN+ NE RELIKKGNRELEPSIVDPRDE TADNWIERNSSMVRLTG
Sbjct: 61  MEQEEEDDDSCSEDENDNNELRELIKKGNRELEPSIVDPRDEATADNWIERNSSMVRLTG 120

Query: 121 KHPFNSEPPLNRLMHHGFITPVPLHYVRNHGAVPKAKWSDWSIEICGLVKRPAKFTMDQL 180
           KHPFNSEPPLNRLMHHGFITPVPLHYVRNHGAVPKA WSDW+IE+CGLVKRP KFTMDQL
Sbjct: 121 KHPFNSEPPLNRLMHHGFITPVPLHYVRNHGAVPKANWSDWTIEVCGLVKRPTKFTMDQL 180

Query: 181 VNEFRFREFPATLVCAGNRRKEQNMVKKTIGFNWGAAGVSTSVWRGVPLRDVLKRCGIFS 240
           VNEFRFREFPATLVCAGNRRKEQNMVKK+IGFNWGAAGVSTSVWRGVPLRDVLKRCGI S
Sbjct: 181 VNEFRFREFPATLVCAGNRRKEQNMVKKSIGFNWGAAGVSTSVWRGVPLRDVLKRCGIMS 240

Query: 241 RKKGALNVCFEGAEDLPGGGGSKYGTSIKKELAMDPARDIILAYMQNGEQLSPDHGFPVR 300
           RKKGALNVCFEGAEDLPGGGGSKYGTSIKKELAMDPARDIILAYMQNGEQL PDHGFPVR
Sbjct: 241 RKKGALNVCFEGAEDLPGGGGSKYGTSIKKELAMDPARDIILAYMQNGEQLVPDHGFPVR 300

Query: 301 MIIPGFIGGRMVKWLKRIIVTTKESENYYHYKDNRVLPSHVDAELANSEAWWYKPEYIIN 360
           MIIPGFIGGRMVKWLKRIIVTTKESENYYH+KDNRVLPSHVDAELAN+EAWWYKPEYIIN
Sbjct: 301 MIIPGFIGGRMVKWLKRIIVTTKESENYYHFKDNRVLPSHVDAELANAEAWWYKPEYIIN 360

Query: 361 ELNINSVITTPCHEEILPINSWTTQRPYTLRGYSYSGGGKKVTRVEVTMDSGETWHVCTL 420
           ELNINSVITTPCHEEILPINSWTTQRPYTLRGYSYSGGGKKVTRVEVTMD GETW VCTL
Sbjct: 361 ELNINSVITTPCHEEILPINSWTTQRPYTLRGYSYSGGGKKVTRVEVTMDGGETWQVCTL 420

Query: 421 DHPEKPNKFGKYWCWCFWSLEVEVLDLLGAKEIAVRAWDETLNTQPEKLIWNLMGMMNNC 480
           DHPEKPNK+GKYWCWCFWSLEVEVLDLLGAKEIAVRAWDETLNTQPEKLIWNLMGMMNNC
Sbjct: 421 DHPEKPNKYGKYWCWCFWSLEVEVLDLLGAKEIAVRAWDETLNTQPEKLIWNLMGMMNNC 480

Query: 481 WFRVKTNVCKPHKGEIGIIFEHPTLPGNQSGGWMDRERHLEISSESNQTLKKSVSTPFMN 540
           WF VKTNVCKPHKGEIGIIFEHPT+PGNQSGGWMDRERHLEIS+ESNQTLKKS+STPFMN
Sbjct: 481 WFTVKTNVCKPHKGEIGIIFEHPTVPGNQSGGWMDRERHLEISTESNQTLKKSISTPFMN 540

Query: 541 TASNTYSLSEVKKHNSPQSAWIIVHGHVYDCTRFLKDHPGGSDSILINAGTDCTEEFDAI 600
           TASNTYSLSEVKKHNSPQSAWIIVHGHVYDCTRFLKDHPGGSDSILINAGTDCTEEFDAI
Sbjct: 541 TASNTYSLSEVKKHNSPQSAWIIVHGHVYDCTRFLKDHPGGSDSILINAGTDCTEEFDAI 600

Query: 601 HSDKAKKMLEDYRIGELITTGYASDSSSNSPNNSTHGASNFSHLAPIKEITPSSPIPARR 660
           HSDKAKKMLEDYRIGELITTGYASDSSSNSPNNSTHGASNFSHLAPI+E    +P+  RR
Sbjct: 601 HSDKAKKMLEDYRIGELITTGYASDSSSNSPNNSTHGASNFSHLAPIRE----APVVTRR 660

Query: 661 VALAPNQKIPCKLVSKTAISHDVRVFRFALP-GQEQTLGLPVGKHIFICATVDGKLCMRA 720
           VALAPN+KIPCKL+SKTAISHDVRVFRF LP GQ+Q LGLPVGKHIFICA V+GKLCMRA
Sbjct: 661 VALAPNEKIPCKLISKTAISHDVRVFRFELPGGQDQVLGLPVGKHIFICAKVEGKLCMRA 720

Query: 721 YTPTSSIDEVGFFELVVKVYFKNVHPKFPNGGVMSQYLESMEVGSTLDVKGPLGHIEYTG 780
           YTP++++D++G+FELVVKVYF+NVHPKFPNGG+MSQ+L++MEVGST++VKGPLGHIEYTG
Sbjct: 721 YTPSTTVDQMGYFELVVKVYFRNVHPKFPNGGIMSQFLDNMEVGSTVEVKGPLGHIEYTG 780

Query: 781 RGNFMVHGKPRFAKRLAMLAGGTGITPIYQVVQAILKDPEDETEMFVVYANRTEEDILLR 840
            GNF VHGKPRFAKRLAMLAGGTGITPIYQVVQAILKDPEDETEMFVVYANRTE+DILLR
Sbjct: 781 SGNFTVHGKPRFAKRLAMLAGGTGITPIYQVVQAILKDPEDETEMFVVYANRTEDDILLR 840

Query: 841 EELEMWAKKNDRLKVWYVVQESIREGWEYSVGFITENILREHIPTAAEDTLALVCGPPPM 900
           EEL+ WAKKN+RLKVWYVVQESIREGWEYS+GFITE I+REH+P AAEDTLALVCGPPPM
Sbjct: 841 EELDTWAKKNERLKVWYVVQESIREGWEYSIGFITEEIMREHLPAAAEDTLALVCGPPPM 900

Query: 901 IQFAVQPNLEKMNYDTKNSMLVF 920
           IQFAVQPNLEKMNYDTKNSMLVF
Sbjct: 901 IQFAVQPNLEKMNYDTKNSMLVF 915

BLAST of Spg008806 vs. ExPASy TrEMBL
Match: A0A1S3CCZ8 (Nitrate reductase OS=Cucumis melo OX=3656 GN=LOC103499266 PE=3 SV=1)

HSP 1 Score: 1776.1 bits (4599), Expect = 0.0e+00
Identity = 844/923 (91.44%), Postives = 887/923 (96.10%), Query Frame = 0

Query: 1   MAASVDNRQFGPLQPPLNGVVRTFKSGPNHRSDSPVRGCNFPNSNIDYSNGNRSVKSSVK 60
           MAASVDNRQFG LQPPLNGVVR+FKSGPN RSDSPVRGCNFPNSNI+    NR +KSSVK
Sbjct: 1   MAASVDNRQFGSLQPPLNGVVRSFKSGPNRRSDSPVRGCNFPNSNIN----NRLLKSSVK 60

Query: 61  IEEEE---DSSSDDENEINEFRELIKKGNRELEPSIVDPRDEGTADNWIERNSSMVRLTG 120
           +E+EE   DS S+DEN+ NE RELIKKGNRELEPSIVDPRDE TADNWIERNSSMVRLTG
Sbjct: 61  MEQEEEDDDSCSEDENDNNELRELIKKGNRELEPSIVDPRDEATADNWIERNSSMVRLTG 120

Query: 121 KHPFNSEPPLNRLMHHGFITPVPLHYVRNHGAVPKAKWSDWSIEICGLVKRPAKFTMDQL 180
           KHPFNSEPPLNRLMHHGFITPVPLHYVRNHGAVPKA WSDW+IE+CGLVKRP KFTMDQL
Sbjct: 121 KHPFNSEPPLNRLMHHGFITPVPLHYVRNHGAVPKANWSDWTIEVCGLVKRPTKFTMDQL 180

Query: 181 VNEFRFREFPATLVCAGNRRKEQNMVKKTIGFNWGAAGVSTSVWRGVPLRDVLKRCGIFS 240
           VNEFRFREFPATLVCAGNRRKEQNMVKK+IGFNWGAAGVSTSVWRGVPLRDVLKRCGI S
Sbjct: 181 VNEFRFREFPATLVCAGNRRKEQNMVKKSIGFNWGAAGVSTSVWRGVPLRDVLKRCGIMS 240

Query: 241 RKKGALNVCFEGAEDLPGGGGSKYGTSIKKELAMDPARDIILAYMQNGEQLSPDHGFPVR 300
           RKKGALNVCFEGAEDLPGGGGSKYGTSIKKELAMDPARDIILAYMQNGEQL PDHGFPVR
Sbjct: 241 RKKGALNVCFEGAEDLPGGGGSKYGTSIKKELAMDPARDIILAYMQNGEQLVPDHGFPVR 300

Query: 301 MIIPGFIGGRMVKWLKRIIVTTKESENYYHYKDNRVLPSHVDAELANSEAWWYKPEYIIN 360
           MIIPGFIGGRMVKWLKRIIVTTKESENYYH+KDNRVLPSHVDAELAN+EAWWYKPEYIIN
Sbjct: 301 MIIPGFIGGRMVKWLKRIIVTTKESENYYHFKDNRVLPSHVDAELANAEAWWYKPEYIIN 360

Query: 361 ELNINSVITTPCHEEILPINSWTTQRPYTLRGYSYSGGGKKVTRVEVTMDSGETWHVCTL 420
           ELNINSVITTPCHEEILPINSWTTQRPYTLRGYSYSGGGKKVTRVEVTMD GETW VCTL
Sbjct: 361 ELNINSVITTPCHEEILPINSWTTQRPYTLRGYSYSGGGKKVTRVEVTMDGGETWQVCTL 420

Query: 421 DHPEKPNKFGKYWCWCFWSLEVEVLDLLGAKEIAVRAWDETLNTQPEKLIWNLMGMMNNC 480
           DHPEKPNK+GKYWCWCFWSLEVEVLDLLGAKEIAVRAWDETLNTQPEKLIWNLMGMMNNC
Sbjct: 421 DHPEKPNKYGKYWCWCFWSLEVEVLDLLGAKEIAVRAWDETLNTQPEKLIWNLMGMMNNC 480

Query: 481 WFRVKTNVCKPHKGEIGIIFEHPTLPGNQSGGWMDRERHLEISSESNQTLKKSVSTPFMN 540
           WF VKTNVCKPHKGEIGIIFEHPT+PGNQSGGWMDRERHLEIS+ESNQTLKKS+STPFMN
Sbjct: 481 WFTVKTNVCKPHKGEIGIIFEHPTVPGNQSGGWMDRERHLEISTESNQTLKKSISTPFMN 540

Query: 541 TASNTYSLSEVKKHNSPQSAWIIVHGHVYDCTRFLKDHPGGSDSILINAGTDCTEEFDAI 600
           TASNTYSLSEVKKHNSPQSAWIIVHGHVYDCTRFLKDHPGGSDSILINAGTDCTEEFDAI
Sbjct: 541 TASNTYSLSEVKKHNSPQSAWIIVHGHVYDCTRFLKDHPGGSDSILINAGTDCTEEFDAI 600

Query: 601 HSDKAKKMLEDYRIGELITTGYASDSSSNSPNNSTHGASNFSHLAPIKEITPSSPIPARR 660
           HSDKAKKMLEDYRIGELITTGYASDSSSNSPNNSTHGASNFSHLAPI+E    +P+  RR
Sbjct: 601 HSDKAKKMLEDYRIGELITTGYASDSSSNSPNNSTHGASNFSHLAPIRE----APVVTRR 660

Query: 661 VALAPNQKIPCKLVSKTAISHDVRVFRFALP-GQEQTLGLPVGKHIFICATVDGKLCMRA 720
           VALAPN+KIPCKL+SKTAISHDVRVFRF LP GQ+Q LGLPVGKHIFICA V+GKLCMRA
Sbjct: 661 VALAPNEKIPCKLISKTAISHDVRVFRFELPGGQDQVLGLPVGKHIFICAKVEGKLCMRA 720

Query: 721 YTPTSSIDEVGFFELVVKVYFKNVHPKFPNGGVMSQYLESMEVGSTLDVKGPLGHIEYTG 780
           YTP++++D++G+FELVVKVYF+NVHPKFPNGG+MSQ+L++MEVGST++VKGPLGHIEYTG
Sbjct: 721 YTPSTTVDQMGYFELVVKVYFRNVHPKFPNGGIMSQFLDNMEVGSTVEVKGPLGHIEYTG 780

Query: 781 RGNFMVHGKPRFAKRLAMLAGGTGITPIYQVVQAILKDPEDETEMFVVYANRTEEDILLR 840
            GNF VHGKPRFAKRLAMLAGGTGITPIYQVVQAILKDPEDETEMFVVYANRTE+DILLR
Sbjct: 781 SGNFTVHGKPRFAKRLAMLAGGTGITPIYQVVQAILKDPEDETEMFVVYANRTEDDILLR 840

Query: 841 EELEMWAKKNDRLKVWYVVQESIREGWEYSVGFITENILREHIPTAAEDTLALVCGPPPM 900
           EEL+ WAKKN+RLKVWYVVQESIREGWEYS+GFITE I+REH+P AAEDTLALVCGPPPM
Sbjct: 841 EELDTWAKKNERLKVWYVVQESIREGWEYSIGFITEEIMREHLPAAAEDTLALVCGPPPM 900

Query: 901 IQFAVQPNLEKMNYDTKNSMLVF 920
           IQFAVQPNLEKMNYDTKNSMLVF
Sbjct: 901 IQFAVQPNLEKMNYDTKNSMLVF 915

BLAST of Spg008806 vs. ExPASy TrEMBL
Match: E7BXW7 (Nitrate reductase OS=Cucumis sativus OX=3659 PE=2 SV=1)

HSP 1 Score: 1773.4 bits (4592), Expect = 0.0e+00
Identity = 843/923 (91.33%), Postives = 887/923 (96.10%), Query Frame = 0

Query: 1   MAASVDNRQFGPLQPPLNGVVRTFKSGPNHRSDSPVRGCNFPNSNIDYSNGNRSVKSSVK 60
           MAASVDNRQFG LQPPLNGVVR+FKSGPNHRSDSPVRGCNFPNSNI+    NR +KSSVK
Sbjct: 1   MAASVDNRQFGSLQPPLNGVVRSFKSGPNHRSDSPVRGCNFPNSNIN----NRLLKSSVK 60

Query: 61  IEEEE---DSSSDDENEINEFRELIKKGNRELEPSIVDPRDEGTADNWIERNSSMVRLTG 120
           +E+EE   DS S+DEN+ NE RELIKKGNRELEPS VDPRDEGTADNWIERNSSMVRLTG
Sbjct: 61  MEQEEEDDDSCSEDENDNNELRELIKKGNRELEPSTVDPRDEGTADNWIERNSSMVRLTG 120

Query: 121 KHPFNSEPPLNRLMHHGFITPVPLHYVRNHGAVPKAKWSDWSIEICGLVKRPAKFTMDQL 180
           KHPFNSEPPLNRLMHHGFITPVPLHYVRNHGAVPKAKW+DW+IE+CGLVKRP KF+MDQL
Sbjct: 121 KHPFNSEPPLNRLMHHGFITPVPLHYVRNHGAVPKAKWADWTIEVCGLVKRPTKFSMDQL 180

Query: 181 VNEFRFREFPATLVCAGNRRKEQNMVKKTIGFNWGAAGVSTSVWRGVPLRDVLKRCGIFS 240
           VNEFRFREFPATLVCAGNRRKEQNMVKK+IGFNWGAAGVSTSVWRGVPLR+VLKRCGI S
Sbjct: 181 VNEFRFREFPATLVCAGNRRKEQNMVKKSIGFNWGAAGVSTSVWRGVPLREVLKRCGIMS 240

Query: 241 RKKGALNVCFEGAEDLPGGGGSKYGTSIKKELAMDPARDIILAYMQNGEQLSPDHGFPVR 300
           RKKGALNVCFEGAEDLPGGGGSKYGTSIKKELAMDPARDIILAYMQNGEQL+PDHGFPVR
Sbjct: 241 RKKGALNVCFEGAEDLPGGGGSKYGTSIKKELAMDPARDIILAYMQNGEQLAPDHGFPVR 300

Query: 301 MIIPGFIGGRMVKWLKRIIVTTKESENYYHYKDNRVLPSHVDAELANSEAWWYKPEYIIN 360
           MIIPGFIGGRMVKWLKRIIVTTKESENYYH+KDNRVLPSHVDAELAN+EAWWYKPEYIIN
Sbjct: 301 MIIPGFIGGRMVKWLKRIIVTTKESENYYHFKDNRVLPSHVDAELANAEAWWYKPEYIIN 360

Query: 361 ELNINSVITTPCHEEILPINSWTTQRPYTLRGYSYSGGGKKVTRVEVTMDSGETWHVCTL 420
           ELNINSVITTPCHEEILPINSWTTQRPYTLRGYSYSGGGKKVTRVEVTMD GET  VCTL
Sbjct: 361 ELNINSVITTPCHEEILPINSWTTQRPYTLRGYSYSGGGKKVTRVEVTMDGGETRQVCTL 420

Query: 421 DHPEKPNKFGKYWCWCFWSLEVEVLDLLGAKEIAVRAWDETLNTQPEKLIWNLMGMMNNC 480
           DHPEKPNK+GKYWCWCFWSLEVEVLDLLGAKEIAVRAWDETLNTQPEKLIWNLMGMMNNC
Sbjct: 421 DHPEKPNKYGKYWCWCFWSLEVEVLDLLGAKEIAVRAWDETLNTQPEKLIWNLMGMMNNC 480

Query: 481 WFRVKTNVCKPHKGEIGIIFEHPTLPGNQSGGWMDRERHLEISSESNQTLKKSVSTPFMN 540
           WF VKTNVCKPHKGEIGIIFEHPT+PGNQSGGWMDRERHLEIS+ESNQTLKKS+STPFMN
Sbjct: 481 WFTVKTNVCKPHKGEIGIIFEHPTVPGNQSGGWMDRERHLEISTESNQTLKKSISTPFMN 540

Query: 541 TASNTYSLSEVKKHNSPQSAWIIVHGHVYDCTRFLKDHPGGSDSILINAGTDCTEEFDAI 600
           TASNTYSLSEVKKHNSPQSAWIIVHGHVYDCTRFLKDHPGGSDSILINAGTDCTEEFDAI
Sbjct: 541 TASNTYSLSEVKKHNSPQSAWIIVHGHVYDCTRFLKDHPGGSDSILINAGTDCTEEFDAI 600

Query: 601 HSDKAKKMLEDYRIGELITTGYASDSSSNSPNNSTHGASNFSHLAPIKEITPSSPIPARR 660
           HSDKAKKMLEDYRIGELITTGYASDSSSNSPNNSTHGASNFSHLAPI+E    +P+  RR
Sbjct: 601 HSDKAKKMLEDYRIGELITTGYASDSSSNSPNNSTHGASNFSHLAPIRE----APMVTRR 660

Query: 661 VALAPNQKIPCKLVSKTAISHDVRVFRFALP-GQEQTLGLPVGKHIFICATVDGKLCMRA 720
            ALAPN+KIPCKL+SKT ISHDVRVFRF LP GQ+Q LGLPVGKHIFICA VDGKLCMRA
Sbjct: 661 AALAPNEKIPCKLISKTEISHDVRVFRFELPGGQDQVLGLPVGKHIFICAKVDGKLCMRA 720

Query: 721 YTPTSSIDEVGFFELVVKVYFKNVHPKFPNGGVMSQYLESMEVGSTLDVKGPLGHIEYTG 780
           YTP+S++D++G+FELVVKVYFKNVHPKFPNGG+MSQ+L++MEVGST++VKGPLGHIEYTG
Sbjct: 721 YTPSSTVDQMGYFELVVKVYFKNVHPKFPNGGIMSQFLDNMEVGSTVEVKGPLGHIEYTG 780

Query: 781 RGNFMVHGKPRFAKRLAMLAGGTGITPIYQVVQAILKDPEDETEMFVVYANRTEEDILLR 840
           RGNF VHGKPRFAKRLAMLAGGTGITPIYQ+VQAILKDPEDETEMFVVYANRTE+DILLR
Sbjct: 781 RGNFTVHGKPRFAKRLAMLAGGTGITPIYQIVQAILKDPEDETEMFVVYANRTEDDILLR 840

Query: 841 EELEMWAKKNDRLKVWYVVQESIREGWEYSVGFITENILREHIPTAAEDTLALVCGPPPM 900
           EEL+ WAKKN+RLKVWYVVQESIREGWEYS+GFITE I REH+P AAEDTLALVCGPPPM
Sbjct: 841 EELDTWAKKNERLKVWYVVQESIREGWEYSIGFITEEITREHLPAAAEDTLALVCGPPPM 900

Query: 901 IQFAVQPNLEKMNYDTKNSMLVF 920
           IQFAVQPNLEKMNYDTKNSMLVF
Sbjct: 901 IQFAVQPNLEKMNYDTKNSMLVF 915

BLAST of Spg008806 vs. TAIR 10
Match: AT1G37130.1 (nitrate reductase 2 )

HSP 1 Score: 1468.0 bits (3799), Expect = 0.0e+00
Identity = 702/931 (75.40%), Postives = 804/931 (86.36%), Query Frame = 0

Query: 1   MAASVDNRQFGPLQPPLNGVVRTFKSGPNHRSDSPVRGCNFPNSNIDYSNGNRSVKSSVK 60
           MAASVDNRQ+  L+P LNGVVR++K     RSDSP    N        +N    +K +  
Sbjct: 1   MAASVDNRQYARLEPGLNGVVRSYKPPVPGRSDSPKAHQN------QTTNQTVFLKPAKV 60

Query: 61  IEEEEDSSSDDENEINE-----FRELIKKGNRELEPSIVDPRDEGTADNWIERNSSMVRL 120
            +++ED SS+DENE +      ++E+I+K N ELEPS++DPRDE TAD+WIERN SMVRL
Sbjct: 61  HDDDEDVSSEDENETHNSNAVYYKEMIRKSNAELEPSVLDPRDEYTADSWIERNPSMVRL 120

Query: 121 TGKHPFNSEPPLNRLMHHGFITPVPLHYVRNHGAVPKAKWSDWSIEICGLVKRPAKFTMD 180
           TGKHPFNSE PLNRLMHHGFITPVPLHYVRNHG VPKA+W++W++E+ G VKRP KFTMD
Sbjct: 121 TGKHPFNSEAPLNRLMHHGFITPVPLHYVRNHGHVPKAQWAEWTVEVTGFVKRPMKFTMD 180

Query: 181 QLVNEFRFREFPATLVCAGNRRKEQNMVKKTIGFNWGAAGVSTSVWRGVPLRDVLKRCGI 240
           QLV+EF +REF ATLVCAGNRRKEQNMVKK+ GFNWG+AGVSTSVWRGVPL DVL+RCGI
Sbjct: 181 QLVSEFAYREFAATLVCAGNRRKEQNMVKKSKGFNWGSAGVSTSVWRGVPLCDVLRRCGI 240

Query: 241 FSRKKGALNVCFEGAEDLPGG---GGSKYGTSIKKELAMDPARDIILAYMQNGEQLSPDH 300
           FSRK GALNVCFEG+EDLPGG    GSKYGTSIKKE AMDP+RDIILAYMQNGE L+PDH
Sbjct: 241 FSRKGGALNVCFEGSEDLPGGAGTAGSKYGTSIKKEYAMDPSRDIILAYMQNGEYLTPDH 300

Query: 301 GFPVRMIIPGFIGGRMVKWLKRIIVTTKESENYYHYKDNRVLPSHVDAELANSEAWWYKP 360
           GFPVR+IIPGFIGGRMVKWLKRIIVTTKES+N+YH+KDNRVLPS VDAELA+ E WWYKP
Sbjct: 301 GFPVRIIIPGFIGGRMVKWLKRIIVTTKESDNFYHFKDNRVLPSLVDAELADEEGWWYKP 360

Query: 361 EYIINELNINSVITTPCHEEILPINSWTTQRPYTLRGYSYSGGGKKVTRVEVTMDSGETW 420
           EYIINELNINSVITTPCHEEILPIN++TTQRPYTL+GY+YSGGGKKVTRVEVT+D GETW
Sbjct: 361 EYIINELNINSVITTPCHEEILPINAFTTQRPYTLKGYAYSGGGKKVTRVEVTVDGGETW 420

Query: 421 HVCTLDHPEKPNKFGKYWCWCFWSLEVEVLDLLGAKEIAVRAWDETLNTQPEKLIWNLMG 480
           +VC LDH EKPNK+GK+WCWCFWSLEVEVLDLL AKEIAVRAWDETLNTQPEK+IWNLMG
Sbjct: 421 NVCALDHQEKPNKYGKFWCWCFWSLEVEVLDLLSAKEIAVRAWDETLNTQPEKMIWNLMG 480

Query: 481 MMNNCWFRVKTNVCKPHKGEIGIIFEHPTLPGNQSGGWMDRERHLEISSESNQTLKKSVS 540
           MMNNCWFRVKTNVCKPHKGEIGI+FEHPTLPGN+SGGWM +ERHLE S+++  +LKKSVS
Sbjct: 481 MMNNCWFRVKTNVCKPHKGEIGIVFEHPTLPGNESGGWMAKERHLEKSADAPPSLKKSVS 540

Query: 541 TPFMNTASNTYSLSEVKKHNSPQSAWIIVHGHVYDCTRFLKDHPGGSDSILINAGTDCTE 600
           TPFMNT +  YS+SEVKKHNS  S WIIVHGH+YDCTRFL DHPGGSDSILINAGTDCTE
Sbjct: 541 TPFMNTTAKMYSMSEVKKHNSADSCWIIVHGHIYDCTRFLMDHPGGSDSILINAGTDCTE 600

Query: 601 EFDAIHSDKAKKMLEDYRIGELITTGYASDSSSNSPNNSTHGASN-FSHLAPIKEITPSS 660
           EF+AIHSDKAKKMLEDYRIGELITTGY+SDSS  SPNNS HG+S  FS LAPI E T   
Sbjct: 601 EFEAIHSDKAKKMLEDYRIGELITTGYSSDSS--SPNNSVHGSSAVFSLLAPIGEAT--- 660

Query: 661 PIPARRVALA-PNQKIPCKLVSKTAISHDVRVFRFALPGQEQTLGLPVGKHIFICATVDG 720
             P R +AL  P  K+P +LV KT+ISHDVR FRFALP ++  LGLPVGKHIF+CAT++ 
Sbjct: 661 --PVRNLALVNPRAKVPVQLVEKTSISHDVRKFRFALPVEDMVLGLPVGKHIFLCATIND 720

Query: 721 KLCMRAYTPTSSIDEVGFFELVVKVYFKNVHPKFPNGGVMSQYLESMEVGSTLDVKGPLG 780
           KLC+RAYTP+S++D VG+FELVVK+YF  VHP+FPNGG+MSQYL+S+ +GSTL++KGPLG
Sbjct: 721 KLCLRAYTPSSTVDVVGYFELVVKIYFGGVHPRFPNGGLMSQYLDSLPIGSTLEIKGPLG 780

Query: 781 HIEYTGRGNFMVHGKPRFAKRLAMLAGGTGITPIYQVVQAILKDPEDETEMFVVYANRTE 840
           H+EY G+G+F VHGKP+FA +LAMLAGGTGITP+YQ++QAILKDPEDETEM+V+YANRTE
Sbjct: 781 HVEYLGKGSFTVHGKPKFADKLAMLAGGTGITPVYQIIQAILKDPEDETEMYVIYANRTE 840

Query: 841 EDILLREELEMWAKK-NDRLKVWYVVQESIREGWEYSVGFITENILREHIPTAAE-DTLA 900
           EDILLREEL+ WA++  DRLKVWYVV ES +EGW YS GFI+E I+REHIP   +   LA
Sbjct: 841 EDILLREELDGWAEQYPDRLKVWYVV-ESAKEGWAYSTGFISEAIMREHIPDGLDGSALA 900

Query: 901 LVCGPPPMIQFAVQPNLEKMNYDTKNSMLVF 920
           + CGPPPMIQFAVQPNLEKM Y+ K   L+F
Sbjct: 901 MACGPPPMIQFAVQPNLEKMQYNIKEDFLIF 917

BLAST of Spg008806 vs. TAIR 10
Match: AT1G77760.1 (nitrate reductase 1 )

HSP 1 Score: 1442.6 bits (3733), Expect = 0.0e+00
Identity = 684/934 (73.23%), Postives = 797/934 (85.33%), Query Frame = 0

Query: 1   MAASVDNRQFGPLQPPLNGVVRTFKSG--PNHRSDSPVRGCNFPNSNIDYSNGNRSVKSS 60
           MA SVDNR +    P +NGV   FK    P+ RS    R     N  +D       +   
Sbjct: 1   MATSVDNRHY----PTMNGVAHAFKPPLVPSPRSFDRHR---HQNQTLDVILTETKIVKE 60

Query: 61  VK-----IEEEEDSSSDDENE-----INEFRELIKKGNRELEPSIVDPRDEGTADNWIER 120
            +     ++  +DSSSDDE+E     +  ++EL+KK N +LEPSI+DPRDE TAD+WI+R
Sbjct: 61  TEVITTVVDSYDDSSSDDEDESHNRNVPYYKELVKKSNSDLEPSILDPRDESTADSWIQR 120

Query: 121 NSSMVRLTGKHPFNSEPPLNRLMHHGFITPVPLHYVRNHGAVPKAKWSDWSIEICGLVKR 180
           NSSM+RLTGKHPFN+E PL RLMHHGFITPVPLHYVRNHGAVPKA WSDWSIEI GLVKR
Sbjct: 121 NSSMLRLTGKHPFNAEAPLPRLMHHGFITPVPLHYVRNHGAVPKANWSDWSIEITGLVKR 180

Query: 181 PAKFTMDQLVNEFRFREFPATLVCAGNRRKEQNMVKKTIGFNWGAAGVSTSVWRGVPLRD 240
           PAKFTM++L++EF  REFP TLVCAGNRRKEQNMVK+TIGFNWG+AGVSTS+W+G+PL +
Sbjct: 181 PAKFTMEELISEFPSREFPVTLVCAGNRRKEQNMVKQTIGFNWGSAGVSTSLWKGIPLSE 240

Query: 241 VLKRCGIFSRKKGALNVCFEGAEDLPGGGGSKYGTSIKKELAMDPARDIILAYMQNGEQL 300
           +L+RCGI+SR+ GALNVCFEGAEDLPGGGGSKYGTSIKKE+AMDPARDIILAYMQNGE L
Sbjct: 241 ILRRCGIYSRRGGALNVCFEGAEDLPGGGGSKYGTSIKKEMAMDPARDIILAYMQNGELL 300

Query: 301 SPDHGFPVRMIIPGFIGGRMVKWLKRIIVTTKESENYYHYKDNRVLPSHVDAELANSEAW 360
           +PDHGFPVR+I+PGFIGGRMVKWLKRIIVT +ES++YYHYKDNRVLPS VDAELANSEAW
Sbjct: 301 TPDHGFPVRVIVPGFIGGRMVKWLKRIIVTPQESDSYYHYKDNRVLPSLVDAELANSEAW 360

Query: 361 WYKPEYIINELNINSVITTPCHEEILPINSWTTQRPYTLRGYSYSGGGKKVTRVEVTMDS 420
           WYKPEYIINELNINSVITTP H EILPIN++TTQ+PYTL+GY+YSGGGKKVTRVEVT+D 
Sbjct: 361 WYKPEYIINELNINSVITTPGHAEILPINAFTTQKPYTLKGYAYSGGGKKVTRVEVTLDG 420

Query: 421 GETWHVCTLDHPEKPNKFGKYWCWCFWSLEVEVLDLLGAKEIAVRAWDETLNTQPEKLIW 480
           G+TW VC LDH EKPNK+GK+WCWCFWSL+VEVLDLL AK++AVRAWDE+ NTQP+KLIW
Sbjct: 421 GDTWSVCELDHQEKPNKYGKFWCWCFWSLDVEVLDLLSAKDVAVRAWDESFNTQPDKLIW 480

Query: 481 NLMGMMNNCWFRVKTNVCKPHKGEIGIIFEHPTLPGNQSGGWMDRERHLEISSESNQTLK 540
           NLMGMMNNCWFR++TNVCKPH+GEIGI+FEHPT PGNQSGGWM +ER LEISSESN TLK
Sbjct: 481 NLMGMMNNCWFRIRTNVCKPHRGEIGIVFEHPTRPGNQSGGWMAKERQLEISSESNNTLK 540

Query: 541 KSVSTPFMNTASNTYSLSEVKKHNSPQSAWIIVHGHVYDCTRFLKDHPGGSDSILINAGT 600
           KSVS+PFMNTAS  YS+SEV+KHN+  SAWIIVHGH+YDCTRFLKDHPGG+DSILINAGT
Sbjct: 541 KSVSSPFMNTASKMYSISEVRKHNTADSAWIIVHGHIYDCTRFLKDHPGGTDSILINAGT 600

Query: 601 DCTEEFDAIHSDKAKKMLEDYRIGELITTGYASDSSSNSPNNSTHGASNFSH-LAPIKEI 660
           DCTEEF+AIHSDKAKK+LEDYRIGELITTGY S     SPN S HGASNF   LAPIKE+
Sbjct: 601 DCTEEFEAIHSDKAKKLLEDYRIGELITTGYDS-----SPNVSVHGASNFGPLLAPIKEL 660

Query: 661 TPSSPIPARRVALAPNQKIPCKLVSKTAISHDVRVFRFALPGQEQTLGLPVGKHIFICAT 720
           TP   I      + P +KIP +L+ KT+ISHDVR FRFALP ++Q LGLPVGKH+F+CA 
Sbjct: 661 TPQKNI----ALVNPREKIPVRLIEKTSISHDVRKFRFALPSEDQQLGLPVGKHVFVCAN 720

Query: 721 VDGKLCMRAYTPTSSIDEVGFFELVVKVYFKNVHPKFPNGGVMSQYLESMEVGSTLDVKG 780
           ++ KLC+RAYTPTS+ID VG  +LVVKVYFK+VHP+FPNGG+MSQ+L+S+ +GS +D+KG
Sbjct: 721 INDKLCLRAYTPTSAIDAVGHIDLVVKVYFKDVHPRFPNGGLMSQHLDSLPIGSMIDIKG 780

Query: 781 PLGHIEYTGRGNFMVHGKPRFAKRLAMLAGGTGITPIYQVVQAILKDPEDETEMFVVYAN 840
           PLGHIEY G+GNF+V GKP+FAK+LAMLAGGTGITPIYQ++Q+IL DPEDETEM+VVYAN
Sbjct: 781 PLGHIEYKGKGNFLVSGKPKFAKKLAMLAGGTGITPIYQIIQSILSDPEDETEMYVVYAN 840

Query: 841 RTEEDILLREELEMWAKKN-DRLKVWYVVQESIREGWEYSVGFITENILREHIPTAAE-D 900
           RTE+DIL+REELE WA K+ +RLK+WYVV E  +EGW YS GFITE +LREHIP   E +
Sbjct: 841 RTEDDILVREELEGWASKHKERLKIWYVV-EIAKEGWSYSTGFITEAVLREHIPEGLEGE 900

Query: 901 TLALVCGPPPMIQFAVQPNLEKMNYDTKNSMLVF 920
           +LAL CGPPPMIQFA+QPNLEKM Y+ K  +L+F
Sbjct: 901 SLALACGPPPMIQFALQPNLEKMGYNVKEDLLIF 917

BLAST of Spg008806 vs. TAIR 10
Match: AT3G01910.1 (sulfite oxidase )

HSP 1 Score: 218.0 bits (554), Expect = 3.1e-56
Identity = 123/383 (32.11%), Postives = 190/383 (49.61%), Query Frame = 0

Query: 113 VRLTGKHPFNSEPPLNRLMHHGFITPVPLHYVRNHGAVPKA-KWSDWSIEICGLVKRPAK 172
           +++  K PFN+EPP + L+   ++TPV L Y RNHG +P       +S+ + GL++ P K
Sbjct: 20  LKVNAKEPFNAEPPRSALV-SSYVTPVDLFYKRNHGPIPIVDHLQSYSVTLTGLIQNPRK 79

Query: 173 FTMDQLVNEFRFREFPATLVCAGNRRKEQNMVKKTIGFNWGAAGVSTSVWRGVPLRDVLK 232
             +  + +  ++    ATL CAGNRR   + V+   G  W  + +  +VW G  L DVL+
Sbjct: 80  LFIKDIRSLPKY-NVTATLQCAGNRRTAMSKVRNVRGVGWDVSAIGNAVWGGAKLADVLE 139

Query: 233 RCGI----FSRKKGALNVCFEGAEDLPGGGGSKYGTSIKKELAMDPARDIILAYMQNGEQ 292
             GI     S   GA +V F   +      G  Y  SI    A +P  D++LAY  NGE 
Sbjct: 140 LVGIPKLTASTNLGARHVEFVSVDRCKEENGGPYKASITLSQATNPEADVLLAYEMNGET 199

Query: 293 LSPDHGFPVRMIIPGFIGGRMVKWLKRIIVTTKESENYYHYKDNRVLPSHVDAELANSEA 352
           L+ DHGFP+R+++PG IG R VKWL  I V  +ES+ ++  KD ++ P  V+        
Sbjct: 200 LNRDHGFPLRVVVPGVIGARSVKWLDSINVIAEESQGFFMQKDYKMFPPSVN-------- 259

Query: 353 WWYKPEYIINELNINSVITTPCHEEILPINSWTTQRP--YTLRGYSYSGGGKKVTRVEVT 412
            W      IN  +    +  P    I  +      +P   +++GY+ SGGG+ + RV+++
Sbjct: 260 -WDN----INWSSRRPQMDFPVQSAICSVEDVQMVKPGKVSIKGYAVSGGGRGIERVDIS 319

Query: 413 MDSGETW-HVCTLDHPEK----PNKFGKYWCWCFWSLEVEVLDLLGAKEIAVRAWDETLN 472
           +D G+ W        P K     +     W W  +   ++V       E+  +A D   N
Sbjct: 320 LDGGKNWVEASRTQEPGKQYISEHSSSDKWAWVLFEATIDVSQ---TTEVIAKAVDSAAN 379

Query: 473 TQPEKL--IWNLMGMMNNCWFRV 482
            QPE +  +WNL G++N  W RV
Sbjct: 380 VQPENVESVWNLRGVLNTSWHRV 384

BLAST of Spg008806 vs. TAIR 10
Match: AT5G17770.1 (NADH:cytochrome B5 reductase 1 )

HSP 1 Score: 176.0 bits (445), Expect = 1.4e-43
Identity = 105/258 (40.70%), Postives = 152/258 (58.91%), Query Frame = 0

Query: 656 RRVALAPNQKIPCKLVSKTAISHDVRVFRFALPGQEQTLGLPVGKHIFICATVD--GKLC 715
           RRV L P      KLV +  +SH+V  F F LP     LGLP+G+HI  C   D  G+  
Sbjct: 38  RRVCLDPENFKEFKLVKRHQLSHNVAKFVFELPTSTSVLGLPIGQHI-SCRGKDGQGEDV 97

Query: 716 MRAYTPTSSIDEVGFFELVVKVYFKNVHPKFPNGGVMSQYLESMEVGSTLDVKGPLGHIE 775
           ++ YTPT+   +VG FELV+K+Y     P+    G MS +   M VG  L VKGP G  +
Sbjct: 98  IKPYTPTTLDSDVGRFELVIKMY-----PQ----GRMSHHFREMRVGDHLAVKGPKGRFK 157

Query: 776 YTGRGNFMVHGKPRFAKRLAMLAGGTGITPIYQVVQAILKDPEDETEMFVVYANRTEEDI 835
           Y          +P   +   MLAGG+GITP++QV +AIL++P D+T++ ++YAN T +DI
Sbjct: 158 Y----------QPGQFRAFGMLAGGSGITPMFQVARAILENPTDKTKVHLIYANVTYDDI 217

Query: 836 LLREELE-MWAKKNDRLKVWYVVQESIREGWEYSVGFITENILREHIPTAAEDTLALVCG 895
           LL+EELE +     ++ K++YV+ +   E W+  VGF+++ +++ H P  A D   L CG
Sbjct: 218 LLKEELEGLTTNYPEQFKIFYVLNQP-PEVWDGGVGFVSKEMIQTHCPAPASDIQILRCG 273

Query: 896 PPPMIQFAVQPNLEKMNY 911
           PPPM + A+  NLE + Y
Sbjct: 278 PPPMNK-AMAANLEALGY 273

BLAST of Spg008806 vs. TAIR 10
Match: AT3G01910.2 (sulfite oxidase )

HSP 1 Score: 171.4 bits (433), Expect = 3.4e-42
Identity = 97/303 (32.01%), Postives = 145/303 (47.85%), Query Frame = 0

Query: 192 CAGNRRKEQNMVKKTIGFNWGAAGVSTSVWRGVPLRDVLKRCGI----FSRKKGALNVCF 251
           CAGNRR   + V+   G  W  + +  +VW G  L DVL+  GI     S   GA +V F
Sbjct: 3   CAGNRRTAMSKVRNVRGVGWDVSAIGNAVWGGAKLADVLELVGIPKLTASTNLGARHVEF 62

Query: 252 EGAEDLPGGGGSKYGTSIKKELAMDPARDIILAYMQNGEQLSPDHGFPVRMIIPGFIGGR 311
              +      G  Y  SI    A +P  D++LAY  NGE L+ DHGFP+R+++PG IG R
Sbjct: 63  VSVDRCKEENGGPYKASITLSQATNPEADVLLAYEMNGETLNRDHGFPLRVVVPGVIGAR 122

Query: 312 MVKWLKRIIVTTKESENYYHYKDNRVLPSHVDAELANSEAWWYKPEYIINELNINSVITT 371
            VKWL  I V  +ES+ ++  KD ++ P  V+         W      IN  +    +  
Sbjct: 123 SVKWLDSINVIAEESQGFFMQKDYKMFPPSVN---------WDN----INWSSRRPQMDF 182

Query: 372 PCHEEILPINSWTTQRP--YTLRGYSYSGGGKKVTRVEVTMDSGETW-HVCTLDHPEK-- 431
           P    I  +      +P   +++GY+ SGGG+ + RV++++D G+ W        P K  
Sbjct: 183 PVQSAICSVEDVQMVKPGKVSIKGYAVSGGGRGIERVDISLDGGKNWVEASRTQEPGKQY 242

Query: 432 --PNKFGKYWCWCFWSLEVEVLDLLGAKEIAVRAWDETLNTQPEKL--IWNLMGMMNNCW 482
              +     W W  +   ++V       E+  +A D   N QPE +  +WNL G++N  W
Sbjct: 243 ISEHSSSDKWAWVLFEATIDVSQ---TTEVIAKAVDSAANVQPENVESVWNLRGVLNTSW 289

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038907222.10.0e+0092.31nitrate reductase [NADH] [Benincasa hispida][more]
NP_001267696.10.0e+0091.55nitrate reductase [NADH]-like [Cucumis sativus] >ADN96689.1 nitrate reductase 2 ... [more]
TYK26501.10.0e+0091.55nitrate reductase [Cucumis melo var. makuwa][more]
XP_008460453.10.0e+0091.44PREDICTED: nitrate reductase [NADH] [Cucumis melo] >KAA0057717.1 nitrate reducta... [more]
ADK77877.10.0e+0091.33nitrate reductase [Cucumis sativus][more]
Match NameE-valueIdentityDescription
P175690.0e+0091.03Nitrate reductase [NADH] OS=Cucurbita maxima OX=3661 PE=2 SV=1[more]
P277830.0e+0078.70Nitrate reductase [NAD(P)H] OS=Betula pendula OX=3505 GN=NIA1 PE=2 SV=1[more]
P368590.0e+0078.61Nitrate reductase [NADH] OS=Petunia hybrida OX=4102 GN=NIA PE=2 SV=1[more]
P085090.0e+0078.28Nitrate reductase [NADH] 2 OS=Nicotiana tabacum OX=4097 GN=NIA2 PE=2 SV=2[more]
P116050.0e+0077.63Nitrate reductase [NADH] 1 OS=Nicotiana tabacum OX=4097 GN=NIA1 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
E2IZV50.0e+0091.55Nitrate reductase OS=Cucumis sativus OX=3659 GN=NR2 PE=2 SV=1[more]
A0A5D3DS070.0e+0091.55Nitrate reductase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold313G00... [more]
A0A5A7USP60.0e+0091.44Nitrate reductase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold1991G0... [more]
A0A1S3CCZ80.0e+0091.44Nitrate reductase OS=Cucumis melo OX=3656 GN=LOC103499266 PE=3 SV=1[more]
E7BXW70.0e+0091.33Nitrate reductase OS=Cucumis sativus OX=3659 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
AT1G37130.10.0e+0075.40nitrate reductase 2 [more]
AT1G77760.10.0e+0073.23nitrate reductase 1 [more]
AT3G01910.13.1e-5632.11sulfite oxidase [more]
AT5G17770.11.4e-4340.70NADH:cytochrome B5 reductase 1 [more]
AT3G01910.23.4e-4232.01sulfite oxidase [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 61..81
NoneNo IPR availableGENE3D2.60.40.650coord: 358..534
e-value: 2.4E-63
score: 215.2
NoneNo IPR availableGENE3D2.40.30.10Translation factorscoord: 655..769
e-value: 3.5E-41
score: 141.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 36..56
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..75
NoneNo IPR availablePANTHERPTHR19370NADH-CYTOCHROME B5 REDUCTASEcoord: 73..919
NoneNo IPR availablePANTHERPTHR19370:SF198NITRATE REDUCTASEcoord: 73..919
NoneNo IPR availableCDDcd02112eukary_NR_Mococoord: 98..483
e-value: 0.0
score: 767.701
NoneNo IPR availableCDDcd06183cyt_b5_reduct_likecoord: 668..919
e-value: 1.16435E-107
score: 330.298
IPR001709Flavoprotein pyridine nucleotide cytochrome reductasePRINTSPR00371FPNCRcoord: 831..842
score: 46.17
coord: 574..584
score: 38.56
coord: 748..757
score: 28.74
coord: 887..895
score: 45.08
coord: 793..812
score: 47.76
coord: 819..828
score: 37.36
coord: 715..722
score: 58.91
coord: 863..879
score: 29.21
IPR001834NADH:cytochrome b5 reductase-likePRINTSPR00406CYTB5RDTASEcoord: 694..705
score: 71.53
coord: 831..842
score: 63.72
coord: 793..812
score: 72.6
coord: 715..722
score: 80.21
coord: 760..774
score: 66.67
coord: 887..895
score: 75.0
IPR008335Eukaryotic molybdopterin oxidoreductasePRINTSPR00407EUMOPTERINcoord: 188..199
score: 91.11
coord: 135..151
score: 80.26
coord: 305..318
score: 83.81
coord: 199..211
score: 76.07
coord: 214..229
score: 64.17
coord: 466..479
score: 75.87
coord: 387..401
score: 73.19
coord: 272..288
score: 70.46
coord: 428..438
score: 74.14
coord: 289..302
score: 78.41
coord: 453..465
score: 70.77
coord: 113..127
score: 82.67
coord: 401..413
score: 65.3
coord: 356..368
score: 75.9
IPR001199Cytochrome b5-like heme/steroid binding domainPRINTSPR00363CYTOCHROMEB5coord: 590..597
score: 64.2
coord: 602..614
score: 61.54
coord: 575..589
score: 68.48
coord: 565..575
score: 70.66
IPR001199Cytochrome b5-like heme/steroid binding domainSMARTSM01117Cyt_b5_2coord: 543..615
e-value: 2.2E-29
score: 113.6
IPR001199Cytochrome b5-like heme/steroid binding domainPFAMPF00173Cyt-b5coord: 544..614
e-value: 6.7E-25
score: 87.0
IPR001199Cytochrome b5-like heme/steroid binding domainPROSITEPS50255CYTOCHROME_B5_2coord: 540..615
score: 27.806641
IPR039261Ferredoxin-NADP reductase (FNR), nucleotide-binding domainGENE3D3.40.50.80coord: 770..919
e-value: 2.6E-47
score: 162.3
IPR039261Ferredoxin-NADP reductase (FNR), nucleotide-binding domainSUPERFAMILY52343Ferredoxin reductase-like, C-terminal NADP-linked domaincoord: 762..914
IPR005066Moybdenum cofactor oxidoreductase, dimerisationPFAMPF03404Mo-co_dimercoord: 354..484
e-value: 9.3E-53
score: 177.9
IPR000572Oxidoreductase, molybdopterin-binding domainPFAMPF00174Oxidored_molybcoord: 147..326
e-value: 3.0E-55
score: 186.4
IPR012137Nitrate reductase NADH dependentPIRSFPIRSF000233Nitr_rd_NADHcoord: 34..918
e-value: 0.0
score: 1283.3
IPR001433Oxidoreductase FAD/NAD(P)-bindingPFAMPF00175NAD_binding_1coord: 794..900
e-value: 1.8E-33
score: 115.4
IPR008333Flavoprotein pyridine nucleotide cytochrome reductase-like, FAD-binding domainPFAMPF00970FAD_binding_6coord: 668..774
e-value: 1.3E-35
score: 121.7
IPR036374Oxidoreductase, molybdopterin-binding domain superfamilyGENE3D3.90.420.10coord: 117..357
e-value: 1.9E-91
score: 307.3
IPR036374Oxidoreductase, molybdopterin-binding domain superfamilySUPERFAMILY56524Oxidoreductase molybdopterin-binding domaincoord: 95..356
IPR036400Cytochrome b5-like heme/steroid binding domain superfamilyGENE3D3.10.120.10coord: 537..617
e-value: 2.4E-29
score: 103.2
IPR036400Cytochrome b5-like heme/steroid binding domain superfamilySUPERFAMILY55856Cytochrome b5-like heme/steroid binding domaincoord: 540..617
IPR018506Cytochrome b5, heme-binding sitePROSITEPS00191CYTOCHROME_B5_1coord: 571..578
IPR022407Oxidoreductase, molybdopterin binding sitePROSITEPS00559MOLYBDOPTERIN_EUKcoord: 164..199
IPR017927FAD-binding domain, ferredoxin reductase-typePROSITEPS51384FAD_FRcoord: 663..775
score: 15.595819
IPR017938Riboflavin synthase-like beta-barrelSUPERFAMILY63380Riboflavin synthase domain-likecoord: 658..775
IPR014756Immunoglobulin E-setSUPERFAMILY81296E set domainscoord: 358..485

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg008806.1Spg008806.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042128 nitrate assimilation
biological_process GO:0006809 nitric oxide biosynthetic process
molecular_function GO:0071949 FAD binding
molecular_function GO:0020037 heme binding
molecular_function GO:0030151 molybdenum ion binding
molecular_function GO:0043546 molybdopterin cofactor binding
molecular_function GO:0009703 nitrate reductase (NADH) activity
molecular_function GO:0050464 nitrate reductase (NADPH) activity
molecular_function GO:0016491 oxidoreductase activity