Spg008556 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg008556
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionReverse transcriptase domain-containing protein
Locationscaffold10: 36941031 .. 36944311 (+)
RNA-Seq ExpressionSpg008556
SyntenySpg008556
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAACATTATTAACAAAGGAATTGTGACTAACCCTTTATGCCTTTTGTGCAGGAATCACGTGGAATGGACTTCCCATCTCTTTTGGGATTGCAAGGTATCAAAAAATTTGTGGCTCAAATACATTCCTCTAACAAAAGATTTATTTGATCTCAACAGGGGATTTTGGAATGCAAAGGCATATTGGCTTTGGCTCATGGACAATCTGAGCGACAAAGAATTGGAAAAATCCGTAATAATTCTCTGGAGCTTATGGCAACATAGAAATGAAGTTCTCACTAATTCCTCCAACGCAGATCAGTTCAGAATTTCAAAGCAAATAGACAGAAATATTTTCGCGCAAGAAGAAATTGACCAATCTCACCGTTCGCGGCTAGGAAGGAAGTCTGAGCAAAGAGTGAAGAGCCAGACGAGTCATGTGCAGTGGCAGCCCCCGCCTGGAAATTGCTGGAAACTCAACGTCGACGCCTCTTGGAGCGAAGCCAGGGGCATTGGAGGGGTGGGGTGGATCCTTCGTGACTCCTCAGGATCTTCACTATGCATGGGTTACAAACTGATCAGCAAAAGCTGGTCTGTCAAAACGCTTGAAATGAAAGCAATTGAAGAAGGCCTCAAATGTGTTCCTTCTTTTCTCGAGCGATCCAGGGAGCAATCCATTGCCCCAATCTTGGTGGAATCTGACGCCATCGGAGTCGTTCGCATCCTTAACGGGGAAGAAGAGGACCTCTCGGAAATCTCTTTTTTGGCCGAGGAGATTCTTCGTCTTAAGGAGTCTTTAGGGGAGGTGTCGTTTCATTTTTGTCCGAGATCTTGCAACGAGGCCGCCCATCGTTTGGCGCGAATTGCAATCTCTGATCCTCCGGTTTCAGTTTCTTTTTCTGGTTTTGAGATCTCTTCAAATGCGGAAGAAGATCATGTTAGTTGGTTTGGCCCTCCCCCTTCGTGGGTTGTTGGGCTATTAGCAGATCTATGTATCACTTCTTTTATTCCTTTTTAATGATATTTCCTATTCAAAAAAAAAAAAGATCCCATATTGAAAAGATAAGTGGAGACCTCACAATTTATAAGATTCATGGGTCACTCTATCCATTTCCAATTGGTTTTGAGATGGAACCCCATATTATCTATTATGGTATCAGAGCCATGAAACCCAAACGGTATTCGAGGCATGTTGAGGATCCCACATTGAAAAGATAAGTGAAGGCCTCACAATTAATAAGATTCATGGGTTACTCCACTCATTGCCAATTAGTTTTGAGATGGAACCCCATATTATATATCTAGGAACATTGTAATGGGTGGCTAAATTCTAATGGTTAATTAATTGCAGATATAACAACTTTTATTAAAAAATTGCTTTAATTTCTATCTGTGATAGATTTCTAACGTGATGGAAATCCAGTGAAGAAATTTATCACTAATAGAAATCAATAGTGTATTTGATTACGTGATAATTGTGTGTGTATATATATATATTTTGAGGTTCCCTCTTCTTTAAATTTCAACTTCCGCTTTGTGTTTTTCTTTTCTTCCAGTTCAATTTCCATGTTGTTTTGCTTCGACATCTATAAAAACAAAATCAGAGACATAGGTAAGGATGTTCACGGGGCGGGAGGGGAACGGGGAAGCCTTCCCCGTCCCCATCCCTGCCCCCTAAACCATTCCTCATCCCCGCAAAATTCCCCATTTAATTTTGCGGGGATCGGGGCGGGGATTCCCCGCGGGGAATTTGCGGGGATTGGGTTCCCCGTTGGGAATTATTCCCCATTTTTTTTAGTTTTTATTAAATTTCAATCAATTGCAATTTTTTTAAAAGATCTATGAAATATCCTAATAAAAAAAAACAATAATATTTCATGATAAAGTTTAAAATCTTCAAACATCATTTCTAAAAGTTTAAATTTCAATATTACAATCTGGTTTTCAACTTAAATATTACACAATTACAACATAGTCTCAAATATAAAAAATCTTAAAATAATAACAACAATAAAATAACAAGTCTAAAATTAAAAATTACAACAACAACAAAGCTAGGAATTTACTACTTATACTTACAAATTACATCATAATATTATAATAATATAAATTAAAGTGTATTATATTATATATATATTATTATTATAAAAAGAGCGGGGCGGGGGGGGGGGGGTCGGGGATGGGGACGGGGAATATAATCCCCGTCCCCGCCCCCGTTTAGAGAATGGGGAAAATTTTCTGCCCACTCCCTCCCCCATTCCCCATCCCGTTCGGGGTGGGGTCCCCACGGGCCCCGGCCCCACGGGGTAAATGGACACCCCTAGACATAAGTTTTAGAAGAAAAAAAAATGATAGTTTTCTATCACTGATAGAAATCTATTAGTGATACGTTTCTATCATTGATAGAAGCCTATCACTTATAGATTTATATCAGTGATAGCTGAAAGAAATCTAACATTGATAGTTCTATTTCTATCACTGATAGAAAACAATAGCAACTTCCAAACAAAATGTAGAAATCTTCTATCGATTCTATTAGTGATAGAAATATAGAAATTTGTCAGTGATAGGTTTCTATCACTGATAGAAATCTACCTTTCTATTACTGATAGGAATCTATTAGTGATGGATTCTATCACTATAGAAACCTATTGTGATACTTTGATATTTTTCAAATAATATTTTTATGTTTTTTTTTTGTTTTTTCAGGTTGATCTTATTAGAATGTACAGTACAAAGTATTGACGGATATAACTGTTGGGTTTTATGTCCTAAAACTCGTGGATGGTAAATGTAACAAAGTTAATCAATAAAGCGTTATTAAGTTTATTCAATAAGTAGTTATTGAATGGTTGTTTAATTCTACATTAATTAACCCAAATCCAAGAAACTAGAACCCATGGCTATATAATGAACACTTGAACTTTATGTGTAGACATAAAAGCGGATCAAGTTCAAGTGATAGCCAAAACGGTCTATAGTATAGGGATAGAGGTTGGGTGTCTTATCCTGGGGACACTATGTGATGCGACCCGCTTTGTATTTATTACAAACGATGTGATCCTGAATCGTTCATGTAACGACATGTGAGTGGGGGCGTCCTATGCAAAGAGTTTGCATAAGACCAGGACCGAGAAATAAGTCACTTTCACTTTATAACACCGTTTACTGTGAAACTGATTATTTCATGCGATGACCTAGGTAACTCGATCTTAATCTTGAGCTAACTATGAACTCCTGTTTATTCAGGATTATCCTTTGATTTGCATGGGTGAGAGTGACCCAACATCGCCGACTCAATAA

mRNA sequence

ATGAACATTATTAACAAAGGAATTGTGACTAACCCTTTATGCCTTTTGTGCAGGAATCACGTGGAATGGACTTCCCATCTCTTTTGGGATTGCAAGGTATCAAAAAATTTGTGGCTCAAATACATTCCTCTAACAAAAGATTTATTTGATCTCAACAGGGGATTTTGGAATGCAAAGGCATATTGGCTTTGGCTCATGGACAATCTGAGCGACAAAGAATTGGAAAAATCCGTAATAATTCTCTGGAGCTTATGGCAACATAGAAATGAAGTTCTCACTAATTCCTCCAACGCAGATCAGTTCAGAATTTCAAAGCAAATAGACAGAAATATTTTCGCGCAAGAAGAAATTGACCAATCTCACCGTTCGCGGCTAGGAAGGAAGTCTGAGCAAAGAGTGAAGAGCCAGACGAGTCATGTGCAGTGGCAGCCCCCGCCTGGAAATTGCTGGAAACTCAACGTCGACGCCTCTTGGAGCGAAGCCAGGGGCATTGGAGGGGTGGGGTGGATCCTTCGTGACTCCTCAGGATCTTCACTATGCATGGGTTACAAACTGATCAGCAAAAGCTGGTCTGTCAAAACGCTTGAAATGAAAGCAATTGAAGAAGGCCTCAAATGTGTTCCTTCTTTTCTCGAGCGATCCAGGGAGCAATCCATTGCCCCAATCTTGGTGGAATCTGACGCCATCGGAGTCGTTCGCATCCTTAACGGGGAAGAAGAGGACCTCTCGGAAATCTCTTTTTTGGCCGAGGAGATTCTTCGTCTTAAGGAGTCTTTAGGGGAGGTGTCGTTTCATTTTTGTCCGAGATCTTGCAACGAGGCCGCCCATCGTTTGGCGCGAATTGCAATCTCTGATCCTCCGGTTTCAGTTTCTTTTTCTGGTTTTGAGATCTCTTCAAATGCGGAAGAAGATCATGATTATCCTTTGATTTGCATGGGTGAGAGTGACCCAACATCGCCGACTCAATAA

Coding sequence (CDS)

ATGAACATTATTAACAAAGGAATTGTGACTAACCCTTTATGCCTTTTGTGCAGGAATCACGTGGAATGGACTTCCCATCTCTTTTGGGATTGCAAGGTATCAAAAAATTTGTGGCTCAAATACATTCCTCTAACAAAAGATTTATTTGATCTCAACAGGGGATTTTGGAATGCAAAGGCATATTGGCTTTGGCTCATGGACAATCTGAGCGACAAAGAATTGGAAAAATCCGTAATAATTCTCTGGAGCTTATGGCAACATAGAAATGAAGTTCTCACTAATTCCTCCAACGCAGATCAGTTCAGAATTTCAAAGCAAATAGACAGAAATATTTTCGCGCAAGAAGAAATTGACCAATCTCACCGTTCGCGGCTAGGAAGGAAGTCTGAGCAAAGAGTGAAGAGCCAGACGAGTCATGTGCAGTGGCAGCCCCCGCCTGGAAATTGCTGGAAACTCAACGTCGACGCCTCTTGGAGCGAAGCCAGGGGCATTGGAGGGGTGGGGTGGATCCTTCGTGACTCCTCAGGATCTTCACTATGCATGGGTTACAAACTGATCAGCAAAAGCTGGTCTGTCAAAACGCTTGAAATGAAAGCAATTGAAGAAGGCCTCAAATGTGTTCCTTCTTTTCTCGAGCGATCCAGGGAGCAATCCATTGCCCCAATCTTGGTGGAATCTGACGCCATCGGAGTCGTTCGCATCCTTAACGGGGAAGAAGAGGACCTCTCGGAAATCTCTTTTTTGGCCGAGGAGATTCTTCGTCTTAAGGAGTCTTTAGGGGAGGTGTCGTTTCATTTTTGTCCGAGATCTTGCAACGAGGCCGCCCATCGTTTGGCGCGAATTGCAATCTCTGATCCTCCGGTTTCAGTTTCTTTTTCTGGTTTTGAGATCTCTTCAAATGCGGAAGAAGATCATGATTATCCTTTGATTTGCATGGGTGAGAGTGACCCAACATCGCCGACTCAATAA

Protein sequence

MNIINKGIVTNPLCLLCRNHVEWTSHLFWDCKVSKNLWLKYIPLTKDLFDLNRGFWNAKAYWLWLMDNLSDKELEKSVIILWSLWQHRNEVLTNSSNADQFRISKQIDRNIFAQEEIDQSHRSRLGRKSEQRVKSQTSHVQWQPPPGNCWKLNVDASWSEARGIGGVGWILRDSSGSSLCMGYKLISKSWSVKTLEMKAIEEGLKCVPSFLERSREQSIAPILVESDAIGVVRILNGEEEDLSEISFLAEEILRLKESLGEVSFHFCPRSCNEAAHRLARIAISDPPVSVSFSGFEISSNAEEDHDYPLICMGESDPTSPTQ
Homology
BLAST of Spg008556 vs. NCBI nr
Match: XP_022154990.1 (uncharacterized protein LOC111022134 isoform X1 [Momordica charantia])

HSP 1 Score: 142.1 bits (357), Expect = 8.2e-30
Identity = 74/223 (33.18%), Postives = 117/223 (52.47%), Query Frame = 0

Query: 18  RNHVEWTSHLFWDCKVSKNLWLKYIPLTKDLFDLNRGFWNAKAYWLWLMDNLSDKELEKS 77
           R   E T H+ W+CKV K++W+   P+  + F ++R  W  K YW WLMD   ++E  +S
Sbjct: 131 RKKEETTQHILWECKVIKDIWINCTPIEANFFYIDRTNWTTKEYWEWLMDKAGEEERRRS 190

Query: 78  VIILWSLWQHRNEVLTNSSNADQFRISKQIDRNIFAQEEIDQSHRSRLGRKSEQ----RV 137
           +II   +W+ RN+ +    +++   I   IDR I      D    + L RKS+     R 
Sbjct: 191 MIIACQIWEMRNKSIFKGVHSETRDIQLAIDRYIINSAGQD----TNLKRKSKDFHPIRR 250

Query: 138 KSQTSHVQWQPPPGNCWKLNVDASWSEARGIGGVGWILRDSSGSSLCMGYKLISKSWSVK 197
               +  +W+PP  N WKLN DA+W       G+GWILRD  G  +  G ++I    ++ 
Sbjct: 251 IGDNTRARWKPPTSNSWKLNTDAAWRADTNTDGIGWILRDEKGEVIKTGCRIIRAERNIT 310

Query: 198 TLEMKAIEEGLKCVPSFLERSREQSIAPILVESDAIGVVRILN 237
            LE+ AI EGL+ +       R++   PI +ESD++  + +L+
Sbjct: 311 YLEVMAICEGLRAI-------RQEHCRPIHLESDSLEAIHLLH 342

BLAST of Spg008556 vs. NCBI nr
Match: XP_022143317.1 (uncharacterized protein LOC111013216 [Momordica charantia])

HSP 1 Score: 124.4 bits (311), Expect = 1.8e-24
Identity = 72/201 (35.82%), Postives = 106/201 (52.74%), Query Frame = 0

Query: 53  RGFWNAKAYWLWLMDNLSDKELEKSVIILWSLWQHRNEVLTNSSNADQFRISKQIDRNI- 112
           R  W  K  W WL++ LSD+E+  S++I W +W+ RN  +      D+    +Q+DR+I 
Sbjct: 10  RVHWTNKDSWNWLVNMLSDEEVATSMVIAWQIWESRNRSIFRGETIDE----QQLDRSIV 69

Query: 113 -FAQEEIDQSHRSRLGRKSEQ--------RVKSQTSHVQWQPPPGNCWKLNVDASWSEAR 172
            F    ID+       R+S+Q        R       V+W  PP NCWKLN DASWSE R
Sbjct: 70  LFINSNIDKGTCISQTRRSQQNDGYLPRGRENLNMLGVRWSAPPTNCWKLNTDASWSEER 129

Query: 173 GIGGVGWILRDSSGSSLCMGYKLISKSWSVKTLEMKAIEEGLKCVPSFLERSREQSIAPI 232
            +GG+GWIL D  G  +  G   I +   +  LE+  I  GL+ +         QS +PI
Sbjct: 130 EVGGIGWILCDCRGEIVLAGNCKIREKKEINALELMTIIRGLQFI-------NMQSRSPI 189

Query: 233 LVESDAIGVVRILNGEEEDLS 244
            +ESD++ V+R++  E+ DL+
Sbjct: 190 YLESDSVEVIRLMKKEDVDLT 199

BLAST of Spg008556 vs. NCBI nr
Match: XP_015385738.1 (uncharacterized protein LOC107177034 [Citrus sinensis])

HSP 1 Score: 120.2 bits (300), Expect = 3.4e-23
Identity = 80/283 (28.27%), Postives = 135/283 (47.70%), Query Frame = 0

Query: 2   NIINKGIVTNPLCLLCRNHVEWTSHLFWDCKVSKNLWLKYIPLTKDLFD-LNRGFWNAKA 61
           N+  + I+  P C +C+   E T+H    CK +K +W +Y P      D +N+   +   
Sbjct: 14  NLWRREILLEPTCQICKQGKETTAHTLLLCKAAKKVW-RYAPFETCFLDAVNQ---DMLE 73

Query: 62  YWLWLMDNLSDKELEKSVIILWSLWQHRNEVLTNSSNADQFRISKQIDRNIFAQEEIDQS 121
             + +   L+  ++E  V I W++W  RN+ L      +      + +  + A   + Q 
Sbjct: 74  IMVEMAKKLTKSDIEVMVAICWTIWYGRNKFLFEGKKMEPLLTISRAEAVVEAYRRVKQP 133

Query: 122 HRSRLGRKSEQRVKSQTSHVQWQPPPGNCWKLNVDASWSEARGIGGVGWILRDSSGSSLC 181
                    E +  S+   V W PPP N +K+NVDA+ +  R   G+G ++RDSS     
Sbjct: 134 TLD-----PEAKYSSKNQQV-WSPPPDNTFKVNVDAAVNFDRQKAGLGVVIRDSSNKVKV 193

Query: 182 MGYKLISKSWSVKTLEMKAIEEGLKCVPSFLERSREQSIAPILVESDAIGVVRILNGEEE 241
              K    +  V+T E +A+E GL         ++  +   I+VESD   VV+++N  E 
Sbjct: 194 AAVKSTLFTGDVQTAEAEAVEWGLVV-------AKSAAFQCIMVESDCQEVVKLINNNEG 253

Query: 242 DLSEISFLAEEILRLKESLGEVSFHFCPRSCNEAAHRLARIAI 284
             +EI ++  EI  L +    +S++  PRSCN  AH LA++A+
Sbjct: 254 SRTEIMWVISEIQSLSKDFQNISYYHTPRSCNTHAHSLAKLAL 279

BLAST of Spg008556 vs. NCBI nr
Match: XP_006491472.1 (uncharacterized protein LOC102626455 [Citrus sinensis])

HSP 1 Score: 117.1 bits (292), Expect = 2.8e-22
Identity = 78/289 (26.99%), Postives = 140/289 (48.44%), Query Frame = 0

Query: 2    NIINKGIVTNPLCLLCRNHVEWTSHLFWDCKVSKNLWLKYIPLTKDLFDLNRGFWNAKAY 61
            N+  +  +  P+C  C+  VE  SH+  +CK ++ +W     + +   D N+ F++A   
Sbjct: 1162 NLWKRRSLQEPICQRCKLQVETVSHVLIECKAARKIWDLAPLIVQPSKDHNQDFFSA-IQ 1221

Query: 62   WLWLMDNLSDKELEKSVIILWSLWQHRNEVLTNSSNADQFRISKQIDRNIFAQEEIDQSH 121
             +W   + ++ EL   ++  W +W  RN+ +     +D   ++ + D  + A + +  S 
Sbjct: 1222 EMWSRSSTAEAEL--MIVYCWVIWSARNKFIFEGKKSDSRFLAAKADSVLKAYQRV--SK 1281

Query: 122  RSRLGRKSEQRVKSQTSHVQWQPPPGNCWKLNVDASWSEARGIGGVGWILRDSSGSSLCM 181
               +    ++ +  Q    +W+PP  N  KLNVDA+ S      G+G I+RD+ G  L +
Sbjct: 1282 PGNVHGAKDRGIDQQ----KWKPPSQNVLKLNVDAAVSTKDQKVGLGAIVRDAEGKILAV 1341

Query: 182  GYKLISKSWSVKTLEMKAIEEGLKCVPSFLERSREQSIAPILVESDAIGVVRILNGEEED 241
            G K       V   E +AI  GL+        + + S + ++VESD   VV +LN  +  
Sbjct: 1342 GIKQAQFRERVSLAEAEAIHWGLQV-------ANQISSSSLIVESDCKEVVELLNNTKGS 1401

Query: 242  LSEISFLAEEILRLKESLGEVSFHFCPRSCNEAAHRLARIAISDPPVSV 291
             +EI ++  ++ R  +   +V F F PR+CN  AH LA+ A+ +    V
Sbjct: 1402 RTEIHWILSDVRRESKEFKQVQFSFIPRTCNTYAHALAKFALRNSSTDV 1434

BLAST of Spg008556 vs. NCBI nr
Match: XP_022155262.1 (uncharacterized protein LOC111022403 [Momordica charantia])

HSP 1 Score: 116.7 bits (291), Expect = 3.7e-22
Identity = 70/218 (32.11%), Postives = 117/218 (53.67%), Query Frame = 0

Query: 65  LMDNLSDKELEKSVIILWSLWQHRNEVLTNSSNADQFRISKQIDRNIFAQEEIDQSHRSR 124
           ++D  SD++L+  +I  W +W HRN V+    ++    + +Q+ +  F  E   QS  S 
Sbjct: 1   MLDKASDEDLDVLLIGSWVIWNHRNYVIFRGEHSSFSTMIQQLTK--FVTESSYQSETS- 60

Query: 125 LGRKSEQRVKSQTSHVQWQPPPGNCWKLNVDASWSEARGIGGVGWILRDSSGSSLCMGYK 184
                    K+  + ++W+PPP + W LN DASWS++   GG+GWI+R   G  +  G +
Sbjct: 61  ----LSMLHKTLNNKLKWEPPPMHIWTLNADASWSDSTHRGGIGWIIRSWDGDIVLAGNR 120

Query: 185 LISKSWSVKTLEMKAIEEGLKCVPSFLERSREQSIAPILVESDAIGVVRILNGEEEDLSE 244
            +    +VK LE  AI EGL+ + +         + P+ +E+D+  V  +LN + EDL++
Sbjct: 121 FVEACNNVKLLEASAILEGLRNLTNL------GVLRPLHIETDSAEVESLLNRKREDLTK 180

Query: 245 ISFLAEEILRLKESLGEVSFHFCPRSCNEAAHRLARIA 283
             ++ EEIL L++S   ++F    R  N  AH LA+ A
Sbjct: 181 TGWVVEEILNLRDSCEILAFAKVGRETNGCAHSLAQRA 205

BLAST of Spg008556 vs. ExPASy TrEMBL
Match: A0A6J1DL64 (uncharacterized protein LOC111022134 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111022134 PE=4 SV=1)

HSP 1 Score: 142.1 bits (357), Expect = 4.0e-30
Identity = 74/223 (33.18%), Postives = 117/223 (52.47%), Query Frame = 0

Query: 18  RNHVEWTSHLFWDCKVSKNLWLKYIPLTKDLFDLNRGFWNAKAYWLWLMDNLSDKELEKS 77
           R   E T H+ W+CKV K++W+   P+  + F ++R  W  K YW WLMD   ++E  +S
Sbjct: 131 RKKEETTQHILWECKVIKDIWINCTPIEANFFYIDRTNWTTKEYWEWLMDKAGEEERRRS 190

Query: 78  VIILWSLWQHRNEVLTNSSNADQFRISKQIDRNIFAQEEIDQSHRSRLGRKSEQ----RV 137
           +II   +W+ RN+ +    +++   I   IDR I      D    + L RKS+     R 
Sbjct: 191 MIIACQIWEMRNKSIFKGVHSETRDIQLAIDRYIINSAGQD----TNLKRKSKDFHPIRR 250

Query: 138 KSQTSHVQWQPPPGNCWKLNVDASWSEARGIGGVGWILRDSSGSSLCMGYKLISKSWSVK 197
               +  +W+PP  N WKLN DA+W       G+GWILRD  G  +  G ++I    ++ 
Sbjct: 251 IGDNTRARWKPPTSNSWKLNTDAAWRADTNTDGIGWILRDEKGEVIKTGCRIIRAERNIT 310

Query: 198 TLEMKAIEEGLKCVPSFLERSREQSIAPILVESDAIGVVRILN 237
            LE+ AI EGL+ +       R++   PI +ESD++  + +L+
Sbjct: 311 YLEVMAICEGLRAI-------RQEHCRPIHLESDSLEAIHLLH 342

BLAST of Spg008556 vs. ExPASy TrEMBL
Match: A0A6J1CQG0 (uncharacterized protein LOC111013216 OS=Momordica charantia OX=3673 GN=LOC111013216 PE=4 SV=1)

HSP 1 Score: 124.4 bits (311), Expect = 8.6e-25
Identity = 72/201 (35.82%), Postives = 106/201 (52.74%), Query Frame = 0

Query: 53  RGFWNAKAYWLWLMDNLSDKELEKSVIILWSLWQHRNEVLTNSSNADQFRISKQIDRNI- 112
           R  W  K  W WL++ LSD+E+  S++I W +W+ RN  +      D+    +Q+DR+I 
Sbjct: 10  RVHWTNKDSWNWLVNMLSDEEVATSMVIAWQIWESRNRSIFRGETIDE----QQLDRSIV 69

Query: 113 -FAQEEIDQSHRSRLGRKSEQ--------RVKSQTSHVQWQPPPGNCWKLNVDASWSEAR 172
            F    ID+       R+S+Q        R       V+W  PP NCWKLN DASWSE R
Sbjct: 70  LFINSNIDKGTCISQTRRSQQNDGYLPRGRENLNMLGVRWSAPPTNCWKLNTDASWSEER 129

Query: 173 GIGGVGWILRDSSGSSLCMGYKLISKSWSVKTLEMKAIEEGLKCVPSFLERSREQSIAPI 232
            +GG+GWIL D  G  +  G   I +   +  LE+  I  GL+ +         QS +PI
Sbjct: 130 EVGGIGWILCDCRGEIVLAGNCKIREKKEINALELMTIIRGLQFI-------NMQSRSPI 189

Query: 233 LVESDAIGVVRILNGEEEDLS 244
            +ESD++ V+R++  E+ DL+
Sbjct: 190 YLESDSVEVIRLMKKEDVDLT 199

BLAST of Spg008556 vs. ExPASy TrEMBL
Match: A0A6J1DNV9 (uncharacterized protein LOC111022403 OS=Momordica charantia OX=3673 GN=LOC111022403 PE=4 SV=1)

HSP 1 Score: 116.7 bits (291), Expect = 1.8e-22
Identity = 70/218 (32.11%), Postives = 117/218 (53.67%), Query Frame = 0

Query: 65  LMDNLSDKELEKSVIILWSLWQHRNEVLTNSSNADQFRISKQIDRNIFAQEEIDQSHRSR 124
           ++D  SD++L+  +I  W +W HRN V+    ++    + +Q+ +  F  E   QS  S 
Sbjct: 1   MLDKASDEDLDVLLIGSWVIWNHRNYVIFRGEHSSFSTMIQQLTK--FVTESSYQSETS- 60

Query: 125 LGRKSEQRVKSQTSHVQWQPPPGNCWKLNVDASWSEARGIGGVGWILRDSSGSSLCMGYK 184
                    K+  + ++W+PPP + W LN DASWS++   GG+GWI+R   G  +  G +
Sbjct: 61  ----LSMLHKTLNNKLKWEPPPMHIWTLNADASWSDSTHRGGIGWIIRSWDGDIVLAGNR 120

Query: 185 LISKSWSVKTLEMKAIEEGLKCVPSFLERSREQSIAPILVESDAIGVVRILNGEEEDLSE 244
            +    +VK LE  AI EGL+ + +         + P+ +E+D+  V  +LN + EDL++
Sbjct: 121 FVEACNNVKLLEASAILEGLRNLTNL------GVLRPLHIETDSAEVESLLNRKREDLTK 180

Query: 245 ISFLAEEILRLKESLGEVSFHFCPRSCNEAAHRLARIA 283
             ++ EEIL L++S   ++F    R  N  AH LA+ A
Sbjct: 181 TGWVVEEILNLRDSCEILAFAKVGRETNGCAHSLAQRA 205

BLAST of Spg008556 vs. ExPASy TrEMBL
Match: A0A6J1DQC9 (uncharacterized protein LOC111022134 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111022134 PE=4 SV=1)

HSP 1 Score: 109.0 bits (271), Expect = 3.7e-20
Identity = 61/185 (32.97%), Postives = 95/185 (51.35%), Query Frame = 0

Query: 56  WNAKAYWLWLMDNLSDKELEKSVIILWSLWQHRNEVLTNSSNADQFRISKQIDRNIFAQE 115
           W  K YW WLMD   ++E  +S+II   +W+ RN+ +    +++   I   IDR I    
Sbjct: 134 WTTKEYWEWLMDKAGEEERRRSMIIACQIWEMRNKSIFKGVHSETRDIQLAIDRYIINSA 193

Query: 116 EIDQSHRSRLGRKSEQ----RVKSQTSHVQWQPPPGNCWKLNVDASWSEARGIGGVGWIL 175
             D    + L RKS+     R     +  +W+PP  N WKLN DA+W       G+GWIL
Sbjct: 194 GQD----TNLKRKSKDFHPIRRIGDNTRARWKPPTSNSWKLNTDAAWRADTNTDGIGWIL 253

Query: 176 RDSSGSSLCMGYKLISKSWSVKTLEMKAIEEGLKCVPSFLERSREQSIAPILVESDAIGV 235
           RD  G  +  G ++I    ++  LE+ AI EGL+ +       R++   PI +ESD++  
Sbjct: 254 RDEKGEVIKTGCRIIRAERNITYLEVMAICEGLRAI-------RQEHCRPIHLESDSLEA 307

Query: 236 VRILN 237
           + +L+
Sbjct: 314 IHLLH 307

BLAST of Spg008556 vs. ExPASy TrEMBL
Match: A0A5B7BI33 (Uncharacterized protein (Fragment) OS=Davidia involucrata OX=16924 GN=Din_037968 PE=4 SV=1)

HSP 1 Score: 108.6 bits (270), Expect = 4.9e-20
Identity = 74/281 (26.33%), Postives = 124/281 (44.13%), Query Frame = 0

Query: 3   IINKGIVTNPLCLLCRNHVEWTSHLFWDCKVSKNLWLKYIPLTKDLFDLNRGFWNAKAYW 62
           ++++ +  +PLC LC N  E   HLF  C   K +W       K  F       + + ++
Sbjct: 85  LVDRHVPVDPLCRLCENGPETPLHLFLHCSWVKLVW----DSAKGSFPDALVHLDFRQFF 144

Query: 63  LWLMDNLSDKELEKSVIILWSLWQHRNEVLTNSSNADQFRISKQIDRNIF-AQEEIDQSH 122
            ++ + L    +E   +I+W +W HRN +  +    D  R+       +F AQ+ +++ H
Sbjct: 145 EYICNKLDSSFIELWCVIIWIVWGHRNAIYHDRQGKDPLRV-------VFDAQQYLNEYH 204

Query: 123 RSRLGRKSEQRVKSQTSHVQWQPPPGNCWKLNVDASWSEARGIGGVGWILRDSSGSSLCM 182
            ++        V S+     W PPP + +KLNVD SW      GG+G ++RDS G  +  
Sbjct: 205 EAQHRLHIPPSVSSRAGSSVWSPPPEDLFKLNVDGSWVPGSFSGGIGGVIRDSRGLVIAG 264

Query: 183 GYKLISKSWSVKTLEMKAIEEGLKCVPSFLERSREQSIAPILVESDAIGVVRILNGEEED 242
             K +    S    E  A+  G+         ++E  I  +L+ESD + +V  +     D
Sbjct: 265 FAKPLKWCGSADYAEACAMFFGVAF-------AKEIGIVDVLIESDCLSLVNSVGCSSPD 324

Query: 243 LSEISFLAEEILRLKESLGEVSFHFCPRSCNEAAHRLARIA 283
            S I  + ++I R    L         RS N+ AH +A  A
Sbjct: 325 FSHIGHITDDIRRGMVGLRSFQVRHVRRSANQTAHEIASFA 347

BLAST of Spg008556 vs. TAIR 10
Match: AT2G34320.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 97.4 bits (241), Expect = 2.2e-20
Identity = 79/279 (28.32%), Postives = 130/279 (46.59%), Query Frame = 0

Query: 14  CLLCRNHVEWTSHLFWDCKVSKNLW-LKYIPLTKDLFDLNRGFWN----AKAYWLWLMDN 73
           C+ C +  E  +HL + C  ++ +W +  IP   +      G W     A  YW+  ++ 
Sbjct: 12  CVRCPDSRETVNHLLFKCCFARLVWAISPIPAYPE------GEWTDSLYANLYWVLNLEV 71

Query: 74  LSDKELEKSVII---LWSLWQHRNEVLTNSSNADQFRISKQIDRNIFAQEEIDQSHRSRL 133
              K  +   ++   LW LW+ RNE++      D    + ++ R      E   + R   
Sbjct: 72  EIPKLGKIGNLVPWLLWRLWKSRNELMFKGKEYD----APEVLRRAMEDFEEWSTRRELE 131

Query: 134 GRKSEQRVKSQTSHVQWQPPPGNCWKLNVDASWSEARGIGGVGWILRDSSGSSLCMGYKL 193
           G+ S  +V+   S VQW+ PP    K N DA+W       G+GWILR+ SG  L MG + 
Sbjct: 132 GKASGPQVERNLS-VQWKAPPYQWVKCNTDATWQLENPRCGIGWILRNESGGVLWMGARA 191

Query: 194 ISKSWSVKTLEMKAIEEGLKCVPSFLERSREQSIAPILVESDAIGVVRILNGEEEDLSEI 253
           + ++ +V   E++A+   +  +  F       +   I+ ESDA  +V +LN  ++    +
Sbjct: 192 LPRTKNVLEAELEALRWAVLTMSRF-------NYKRIIFESDAQALVNLLN-SDDFWPTL 251

Query: 254 SFLAEEILRLKESLGEVSFHFCPRSCNEAAHRLARIAIS 285
               E+I +L     EV F F PR  N+ A R+AR +IS
Sbjct: 252 QPALEDIQQLLHHFEEVKFEFTPRGGNKVADRIARESIS 271

BLAST of Spg008556 vs. TAIR 10
Match: AT5G65005.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 50.4 bits (119), Expect = 3.0e-06
Identity = 44/206 (21.36%), Postives = 91/206 (44.17%), Query Frame = 0

Query: 80  ILWSLWQHRNEVLTNSSNADQFRISKQIDRNIFAQEEIDQSHRSRLGRKSEQRVKSQTSH 139
           ++W +W+  N+++ N +   +F+ + ++  N   +E +D +  +   +++  R    + +
Sbjct: 47  LMWRIWKSGNDLVFNHTRT-KFQTTVEMALN-DTKEWLDNTMTNE--QQNGNRNADPSRN 106

Query: 140 VQWQPPPGNCWKLNVDASWSEARGIGGVGWILRDSSGSSLCMGYKLISKSWSVKTLEMKA 199
            +W PP  +  K N DAS  E   + G+GWILR+S G+ +  G        + +  E   
Sbjct: 107 TKWSPPGRDKLKCNYDASHHERNTVSGLGWILRNSQGTVIECGMGKFQGRMTTEEAECST 166

Query: 200 IEEGLKCVPSFLERSREQSIAPILVESDAIGVVRILNGEEEDLSEISFLAEEILRLKESL 259
           +   ++    F  +        ++ E D   + R++N +  +   +    + I     S 
Sbjct: 167 LIWAIQASYGFGHKK-------VIFEGDNQTITRMINTKSSN-PRLQHFLDTIQSWIPSF 226

Query: 260 GEVSFHFCPRSCNEAAHRLARIAISD 286
             + F F  R  N  A  LA+ AI +
Sbjct: 227 ESIEFSFKHREQNGCADFLAKQAIKE 240

BLAST of Spg008556 vs. TAIR 10
Match: AT4G10613.1 (RNA-directed DNA polymerase (reverse transcriptase)-related family protein )

HSP 1 Score: 48.9 bits (115), Expect = 8.8e-06
Identity = 31/111 (27.93%), Postives = 49/111 (44.14%), Query Frame = 0

Query: 3   IINKGIVTNPLCLLCRNHVEWTSHLFWDCKVSKNLWLKYIPLTKDLFDLNRGFWNAKAYW 62
           +++ G+  +PLC LC   VE   HL   C  S ++W   +     L  +    W +   W
Sbjct: 15  LVSWGLQISPLCCLCSQSVETRDHLILTCGFSSSIW-NMVQARLRLQPVQFRVWESLLSW 74

Query: 63  LWLMDNLSDKELEKSV--IILWSLWQHRNEVLTNSSNADQFRISKQIDRNI 112
           + L    S   + K V    + ++W+ RN +L N  +     I K IDR I
Sbjct: 75  IKLSTASSPSIIRKLVAQATVCAIWKQRNNLLHNLQDILPSIIFKNIDREI 124

BLAST of Spg008556 vs. TAIR 10
Match: AT3G23320.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 46.6 bits (109), Expect = 4.4e-05
Identity = 45/172 (26.16%), Postives = 73/172 (42.44%), Query Frame = 0

Query: 114 QEEIDQSHRSRLGRKSEQRVKSQTSHVQWQPPPGNCWKLNVDASWSEARGIGGVGWILRD 173
           +E +D  H++    K  Q   S+T   +WQ P     K N D S    R   G+ WI+R+
Sbjct: 4   KEWLDVKHKNDDNVKGGQTKSSRTD--KWQKPGAEWVKCNYDVSNHAGRQDSGLRWIIRN 63

Query: 174 SSGSSLCMGYKLISKSWSVKTLEMKAIEEGLKCVPSFLERSREQSIAPILVESDAIGVVR 233
           S G+ L  G        ++K  E  A+   ++C      R  E        E D I V R
Sbjct: 64  SQGTCLDCGCGKFQGRQTIKEAECTALIWAIQCAWDLGYRRVE-------FEGDNITVNR 123

Query: 234 ILNGEEEDLSEISFLAEEILRLKESLGEVSFHFCPRSCNEAAHRLARIAISD 286
           ++  +E +   + +  E I +  ++   V F F  R  N     LA+ A+++
Sbjct: 124 LIRNKETN-PRLRYYLEFIQQWSKAFTTVKFTFRHREQNVCVDVLAKKAVAN 165

BLAST of Spg008556 vs. TAIR 10
Match: AT2G02520.1 (RNA-directed DNA polymerase (reverse transcriptase)-related family protein )

HSP 1 Score: 45.1 bits (105), Expect = 1.3e-04
Identity = 27/103 (26.21%), Postives = 49/103 (47.57%), Query Frame = 0

Query: 3   IINKGIVTNPLCLLCRNHVEWTSHLFWDCKVSKNLWLKYIPLTKDLFD---LNRGFWNAK 62
           +I+ G +  PLCL C  H E   HLF+DC+ ++ +W+ Y      +F       G     
Sbjct: 74  MISWGFIFPPLCLFCNTHDETRQHLFFDCEFAREVWI-YFTSRVHVFPPLLFEDGI---- 133

Query: 63  AYWLWLMDNLSDKELEKSVII-----LWSLWQHRNEVLTNSSN 98
               WL +   DK +   + +     ++++W+ RN  L +S++
Sbjct: 134 ---RWLKNPCQDKNVTTILRLSHHASVYTIWKERNARLHDSAS 168

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022154990.18.2e-3033.18uncharacterized protein LOC111022134 isoform X1 [Momordica charantia][more]
XP_022143317.11.8e-2435.82uncharacterized protein LOC111013216 [Momordica charantia][more]
XP_015385738.13.4e-2328.27uncharacterized protein LOC107177034 [Citrus sinensis][more]
XP_006491472.12.8e-2226.99uncharacterized protein LOC102626455 [Citrus sinensis][more]
XP_022155262.13.7e-2232.11uncharacterized protein LOC111022403 [Momordica charantia][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1DL644.0e-3033.18uncharacterized protein LOC111022134 isoform X1 OS=Momordica charantia OX=3673 G... [more]
A0A6J1CQG08.6e-2535.82uncharacterized protein LOC111013216 OS=Momordica charantia OX=3673 GN=LOC111013... [more]
A0A6J1DNV91.8e-2232.11uncharacterized protein LOC111022403 OS=Momordica charantia OX=3673 GN=LOC111022... [more]
A0A6J1DQC93.7e-2032.97uncharacterized protein LOC111022134 isoform X2 OS=Momordica charantia OX=3673 G... [more]
A0A5B7BI334.9e-2026.33Uncharacterized protein (Fragment) OS=Davidia involucrata OX=16924 GN=Din_037968... [more]
Match NameE-valueIdentityDescription
AT2G34320.12.2e-2028.32Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
AT5G65005.13.0e-0621.36Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
AT4G10613.18.8e-0627.93RNA-directed DNA polymerase (reverse transcriptase)-related family protein [more]
AT3G23320.14.4e-0526.16Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
AT2G02520.11.3e-0426.21RNA-directed DNA polymerase (reverse transcriptase)-related family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 150..286
e-value: 3.6E-14
score: 54.9
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 153..280
e-value: 8.8E-22
score: 77.2
NoneNo IPR availablePANTHERPTHR33033:SF67SUBFAMILY NOT NAMEDcoord: 139..286
NoneNo IPR availablePANTHERPTHR33033POLYNUCLEOTIDYL TRANSFERASE, RIBONUCLEASE H-LIKE SUPERFAMILY PROTEIN-RELATEDcoord: 139..286
IPR044730Ribonuclease H-like domain, plant typeCDDcd06222RNase_H_likecoord: 152..280
e-value: 7.39319E-23
score: 89.6808
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 148..288

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg008556.1Spg008556.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity