Homology
BLAST of Spg008554 vs. NCBI nr
Match:
XP_038875555.1 (probable RNA-dependent RNA polymerase 1 [Benincasa hispida])
HSP 1 Score: 1913.3 bits (4955), Expect = 0.0e+00
Identity = 945/1118 (84.53%), Postives = 1017/1118 (90.97%), Query Frame = 0
Query: 3 TIEIFGFPANVAVDDVKEFLENHTGDGTVSTVRISKPKDENSRFTFATVRFTSELAAEYV 62
TIEI+GF V VD+VKEFLENHTGDGTV TVRISKPKDE +RFTF TVRF SELAAEY+
Sbjct: 4 TIEIYGFRPEVTVDEVKEFLENHTGDGTVLTVRISKPKDEKTRFTFVTVRFKSELAAEYI 63
Query: 63 VEQA-EAEERLWYESSYLKARGVEREMRAAAGRRGEVERIENVRIHLGSRISKERMSVIW 122
VE+A AE++LW+ SSYLKAR VER A G GE+ER+E+V+ HLGS ISK++M VIW
Sbjct: 64 VEKATAAEKKLWFGSSYLKAREVERASPARGG--GEMERMEDVKGHLGSLISKDKMRVIW 123
Query: 123 RGENCCVEFGVGIRKLCFYLSYEFDEYKMELSFENILGVELRCPLDPPSNLLLIQLQGAP 182
GEN VEFG+G+RKL FYLSYE EYKMEL+FENILGVE RCPL+ PS LIQLQGAP
Sbjct: 124 EGENWNVEFGIGVRKLSFYLSYEV-EYKMELAFENILGVEFRCPLNQPSKFFLIQLQGAP 183
Query: 183 RIFRKSSSSSSAQFYSKESAGFRWIRDVDFTPSSSIGQSFTICLEVSPEHQLPSFFETLV 242
RIF+KS SSSS++ S E FRWIRDVDFTPSS IGQSF +CLE+SP H LP FF+ LV
Sbjct: 184 RIFKKSRSSSSSRLNSNEFC-FRWIRDVDFTPSSCIGQSFALCLELSPGHHLPPFFQNLV 243
Query: 243 GYKERYGPFNLETDSSFSSTSNLVPIISPPQGFDFSYKILFKINALVQHGYLPGPVLDDE 302
GYKE Y PF L+T SSFSS SNLVPII+PP+GFD SYK LFKINALVQHGYLPGP LDDE
Sbjct: 244 GYKETYAPFILQTGSSFSSISNLVPIITPPRGFDISYKTLFKINALVQHGYLPGPTLDDE 303
Query: 303 FFRLVDPSRFHPDCVEQALEKLFNLKECCYKPKKWLRQQYLSFYSSKQPSWKPNISLDDG 362
FFRLVD SRF PD VE ALEK+FNLKECCY+P+KWL+ YLSFY+S Q WKPNISLDDG
Sbjct: 304 FFRLVDSSRFRPDYVEHALEKMFNLKECCYEPQKWLKHHYLSFYTSNQLPWKPNISLDDG 363
Query: 363 LVYVHRVQITPSKVYFRGPEANLSNRVVRRFIDDIDNFLRVSFVDEELDKIRSIDLSPR- 422
LVYVHRVQITPSKVYFRGPEANLSNRVVRRFIDDIDNFLRVSFVDE+LDK+ SIDL+PR
Sbjct: 364 LVYVHRVQITPSKVYFRGPEANLSNRVVRRFIDDIDNFLRVSFVDEDLDKLHSIDLAPRS 423
Query: 423 TSAENTERTRVYDRVLSVLRNGIVIGEKKFEFLAFSASQLRENSFWMFASREGLTAADIR 482
+SA N+ERTRVYDR+LSVLRNGIVIG+KKFEFLAFSASQLRENSFWMFASREGL+AADIR
Sbjct: 424 SSAGNSERTRVYDRILSVLRNGIVIGDKKFEFLAFSASQLRENSFWMFASREGLSAADIR 483
Query: 483 EWMGDFRQIRNVAKYAARLGQSFGSSRKTLCVEEDEIEVIPDVEVVRKKIKYCFSDGIGK 542
EWMGDFR+IRNVAKYAARLGQSFGSSRKTLCVEE E EVIPDVEV RKK KYCFSDGIGK
Sbjct: 484 EWMGDFREIRNVAKYAARLGQSFGSSRKTLCVEEHEFEVIPDVEVERKKTKYCFSDGIGK 543
Query: 543 ISETLAEKVAEKCGLISHTPSAFQIRYAGYKGVVAIDPTSKKKLSLRKSMLKYMSLDTQL 602
IS+TLAEKVAEKCGLISHTPSAFQIRYAGYKGVVAIDPT KKKLSLRKSMLKYMSLDTQL
Sbjct: 544 ISKTLAEKVAEKCGLISHTPSAFQIRYAGYKGVVAIDPTLKKKLSLRKSMLKYMSLDTQL 603
Query: 603 DVLSWSKYQPCFLNRQVINLLSTLGIRDHVFVKKQKEAIDQLDSILHDPSKALEVLELMS 662
DVL WSKYQPCFLNRQVINLLSTLGI+D VFVKKQKEAIDQLDSIL DPSKALEVLELMS
Sbjct: 604 DVLLWSKYQPCFLNRQVINLLSTLGIKDRVFVKKQKEAIDQLDSILEDPSKALEVLELMS 663
Query: 663 PGEMTSILKEMLLFYKPNEEPFLNMMLRTFRADKLLDLRTKSRIFVPKGRTMMGCLDETQ 722
PGEMT ILKE+LLFYKPNEEPFLNMMLRTFRADK LDLRTKSRIFVPKGRTMMGCLDET+
Sbjct: 664 PGEMTGILKELLLFYKPNEEPFLNMMLRTFRADKFLDLRTKSRIFVPKGRTMMGCLDETR 723
Query: 723 TLEYGQVFVHCSVPGRSSENSFVVKGKVVVAKNPCLHPGDVRLLDAVDVKALHHMVDCVV 782
TLEYGQVFVHCS+PGRS+E++FV+KGKVVVAKNPCLHPGDVR+LDAVDVKALHHMVDCVV
Sbjct: 724 TLEYGQVFVHCSIPGRSNESNFVIKGKVVVAKNPCLHPGDVRVLDAVDVKALHHMVDCVV 783
Query: 783 FPQKGERPHPNECSGSDLDGDLYFVCWDSKLICIRPVKPMSYEPAPTIQLDHDVTIEEVQ 842
FPQKG+RPHPNECSGSDLDGDLYFVCWD +L CI+PVKPMSYEPAPT+QLDHDVTIEEVQ
Sbjct: 784 FPQKGKRPHPNECSGSDLDGDLYFVCWDPELTCIKPVKPMSYEPAPTMQLDHDVTIEEVQ 843
Query: 843 EYLAKYMVNDGLGGIANAHTVFADKKPKKAMSAECVELAKLFSIAVDFPKTGVPANLPRS 902
EYLA YMVNDGLG IANAHTVFADK PKKAMS EC++LAKLFSIAVDFPKTGVPANLPR+
Sbjct: 844 EYLANYMVNDGLGAIANAHTVFADKNPKKAMSTECIKLAKLFSIAVDFPKTGVPANLPRN 903
Query: 903 LRVHEYPDFMEKPDKPTYVSNGVLGKLFRGVKDVSSDINTIEAFTREVATKCYDPDMEVD 962
LRVHEYPDFM+KP+KPTY SNGVLGKLF+GVKDVSSD+NT+E FTREVA CYDPDMEVD
Sbjct: 904 LRVHEYPDFMDKPNKPTYASNGVLGKLFQGVKDVSSDVNTLEIFTREVAINCYDPDMEVD 963
Query: 963 GFENYLREAFDYKNRYDFKLGNLMDYYGIKTEPELVSGNILRMAKSFDKRNDLEQISLAM 1022
GF+ YL EAFDYK RYDFKLGNLMDYYGIKTEPELVSGNILRMAKSFDKRNDLEQI+ AM
Sbjct: 964 GFDKYLSEAFDYKTRYDFKLGNLMDYYGIKTEPELVSGNILRMAKSFDKRNDLEQITFAM 1023
Query: 1023 KSLRKEARAWFNEKGSKSTYD----EDEEYAKASAWYHVTYHPNYWGRYNKGMQRDHFLS 1082
KSLRKEARAWFNEKGSKSTYD E+EEYAKASAWYHVTYHP+YWGRYN+GMQRDHFLS
Sbjct: 1024 KSLRKEARAWFNEKGSKSTYDNSKGEEEEYAKASAWYHVTYHPDYWGRYNEGMQRDHFLS 1083
Query: 1083 FPWCVSDQLIQIKREKMCQISYSQVSSLIHKFGDLSFY 1115
FPWCV+D+LIQIKREK +S S +SSLIHKFG LS Y
Sbjct: 1084 FPWCVADKLIQIKREKTLLMSSSPMSSLIHKFGSLSLY 1117
BLAST of Spg008554 vs. NCBI nr
Match:
XP_022990904.1 (probable RNA-dependent RNA polymerase 1 [Cucurbita maxima])
HSP 1 Score: 1900.6 bits (4922), Expect = 0.0e+00
Identity = 946/1120 (84.46%), Postives = 1007/1120 (89.91%), Query Frame = 0
Query: 3 TIEIFGFPANVAVDDVKEFLENHTGDGTVSTVRISKPKDENSRFTFATVRFTSELAAEYV 62
TIE+FGF V+ +VKEF+ENHTG+GTVS VRISKPKD R TFA V F S++AAEYV
Sbjct: 7 TIEVFGFRREVSAGEVKEFMENHTGEGTVSKVRISKPKDGKGRLTFARVEFRSKVAAEYV 66
Query: 63 VEQAEAEERLWYESSYLKARGVEREMRAAAGRRGEVERIENVRIHLGSRISKERMSVIWR 122
VE+AEAEE LW+ YLKARGVEREMR VER+E VR+ LGS ISKE M VIWR
Sbjct: 67 VEKAEAEEGLWFGRWYLKARGVEREMR--------VERMEKVRVQLGSVISKEEMRVIWR 126
Query: 123 GENCCVEFGVGIRKLCFYLSY--EFDEYKMELSFENILGVELRCPLDPPSNLLLIQLQGA 182
GE VE+G G+RKL FYLSY E D+YKMEL FENILGV+LRCP + SNL LIQLQGA
Sbjct: 127 GEEWSVEYGNGMRKLWFYLSYGVEDDDYKMELRFENILGVQLRCPHNSSSNLFLIQLQGA 186
Query: 183 PRIFRK--SSSSSSAQFYSKESAGFRWIRDVDFTPSSSIGQSFTICLEVSPEHQLPSFFE 242
PRIF+K SSSSSS+QFYSKES GFRWIRDVDFTPSS IGQSF++CLEVSP QLPSFF+
Sbjct: 187 PRIFKKCPSSSSSSSQFYSKESTGFRWIRDVDFTPSSCIGQSFSLCLEVSPGDQLPSFFQ 246
Query: 243 TLVGYKERYGPFNLETDSSFSSTSNLVPIISPPQGFDFSYKILFKINALVQHGYLPGPVL 302
TLVGYKE YGPF L T S+FSSTSNLVPI++PPQ FD YKILFKINALVQHGYLPG L
Sbjct: 247 TLVGYKEIYGPFKLSTGSAFSSTSNLVPIVTPPQPFDIPYKILFKINALVQHGYLPGSAL 306
Query: 303 DDEFFRLVDPSRFHPDCVEQALEKLFNLKECCYKPKKWLRQQYLSFYSSKQPSWKPNISL 362
DD+FFRLVDPSRF D +E ALEKLF+LKECCY+P+KWL+QQYLSF SS Q WK NISL
Sbjct: 307 DDKFFRLVDPSRFRSDYIEYALEKLFSLKECCYEPQKWLKQQYLSFCSSMQLPWKANISL 366
Query: 363 DDGLVYVHRVQITPSKVYFRGPEANLSNRVVRRFIDDIDNFLRVSFVDEELDKIRSIDLS 422
DD LVYVHRVQITP+KVYFRGPEANLSNRVVRRFIDDIDNFLRVSFVDEELDKI S DLS
Sbjct: 367 DDSLVYVHRVQITPAKVYFRGPEANLSNRVVRRFIDDIDNFLRVSFVDEELDKIHSSDLS 426
Query: 423 PRTSAENTERTRVYDRVLSVLRNGIVIGEKKFEFLAFSASQLRENSFWMFASREGLTAAD 482
PRTS E ERTRVYDR+LSVLRNGIVIG KKFEFLAFSASQLRENSFWMFASREGL AAD
Sbjct: 427 PRTSTEKIERTRVYDRILSVLRNGIVIGGKKFEFLAFSASQLRENSFWMFASREGLNAAD 486
Query: 483 IREWMGDFRQIRNVAKYAARLGQSFGSSRKTLCVEEDEIEVIPDVEVVRKKIKYCFSDGI 542
IREWMGDFR+IRNVAKYAARLGQSFGSSRKTLCVEE+EIEVIPD EV RK IKYCFSDGI
Sbjct: 487 IREWMGDFREIRNVAKYAARLGQSFGSSRKTLCVEENEIEVIPDAEVERKNIKYCFSDGI 546
Query: 543 GKISETLAEKVAEKCGLISHTPSAFQIRYAGYKGVVAIDPTSKKKLSLRKSMLKYMSLDT 602
GKISE LAE VAEKCGLISHTPSAFQIRYAGYKGVVA+DPTSKKKLSLRKSMLKYMSLDT
Sbjct: 547 GKISEALAEMVAEKCGLISHTPSAFQIRYAGYKGVVAVDPTSKKKLSLRKSMLKYMSLDT 606
Query: 603 QLDVLSWSKYQPCFLNRQVINLLSTLGIRDHVFVKKQKEAIDQLDSILHDPSKALEVLEL 662
QLDVLSWSKYQPCFLNRQVINLLSTLGIRD VFVK+QK+A+DQLDSIL DPSKALEVLEL
Sbjct: 607 QLDVLSWSKYQPCFLNRQVINLLSTLGIRDDVFVKQQKKAVDQLDSILRDPSKALEVLEL 666
Query: 663 MSPGEMTSILKEMLLFYKPNEEPFLNMMLRTFRADKLLDLRTKSRIFVPKGRTMMGCLDE 722
MSPGEMTS+LK+MLLFY P+EEPFLNMMLRTFRADKLLDLRTKSRIFVPKGRTMMGCLDE
Sbjct: 667 MSPGEMTSVLKDMLLFYNPHEEPFLNMMLRTFRADKLLDLRTKSRIFVPKGRTMMGCLDE 726
Query: 723 TQTLEYGQVFVHCSVPGRSSENSFVVKGKVVVAKNPCLHPGDVRLLDAVDVKALHHMVDC 782
T+TLEYGQVF+HCSVPGRSSE++FVVKGKVVVAKNPCLHPGDVR+LDAVDVK LHHMVDC
Sbjct: 727 TRTLEYGQVFIHCSVPGRSSESNFVVKGKVVVAKNPCLHPGDVRVLDAVDVKTLHHMVDC 786
Query: 783 VVFPQKGERPHPNECSGSDLDGDLYFVCWDSKLICIRPVKPMSYEPAPTIQLDHDVTIEE 842
VVFPQKG+RPHPNECSGSDLDGDLYFVCWD +L CIRP+KPMSYEPAPTIQLDHDVT+EE
Sbjct: 787 VVFPQKGKRPHPNECSGSDLDGDLYFVCWDPELTCIRPIKPMSYEPAPTIQLDHDVTLEE 846
Query: 843 VQEYLAKYMVNDGLGGIANAHTVFADKKPKKAMSAECVELAKLFSIAVDFPKTGVPANLP 902
VQEY A YMVNDGLGGIANAHTVFAD KPKKAMSAEC+ELAKLFSIAVDFPKTGVPANLP
Sbjct: 847 VQEYFANYMVNDGLGGIANAHTVFADMKPKKAMSAECIELAKLFSIAVDFPKTGVPANLP 906
Query: 903 RSLRVHEYPDFMEKPDKPTYVSNGVLGKLFRGVKDVSSDINTIEAFTREVATKCYDPDME 962
RSLRVHEYPDFMEKP+K TY+SNGV+GKLFRGVKDVSSDIN IE FTREVA KCYDPDME
Sbjct: 907 RSLRVHEYPDFMEKPNKRTYISNGVIGKLFRGVKDVSSDINNIETFTREVAVKCYDPDME 966
Query: 963 VDGFENYLREAFDYKNRYDFKLGNLMDYYGIKTEPELVSGNILRMAKSFDKRNDLEQISL 1022
V GFENYL EAFDYK YDFKLGNLMDYYGI+TEPELVSGNILRM KSFDKRND+EQISL
Sbjct: 967 VMGFENYLSEAFDYKRMYDFKLGNLMDYYGIETEPELVSGNILRMGKSFDKRNDMEQISL 1026
Query: 1023 AMKSLRKEARAWFNEKGSKSTYD----EDEEYAKASAWYHVTYHPNYWGRYNKGMQRDHF 1082
AM+SLRKEAR WFNEK SKS Y+ ED EYAKASAWYHVTYHPN+WGRYN+GMQRDHF
Sbjct: 1027 AMRSLRKEARGWFNEKASKSIYNNNKGEDNEYAKASAWYHVTYHPNFWGRYNEGMQRDHF 1086
Query: 1083 LSFPWCVSDQLIQIKREKMCQISYSQVSSLIHKFGDLSFY 1115
LSFPWCVSD+LIQIKREKM S VSSLIHKFG LSFY
Sbjct: 1087 LSFPWCVSDKLIQIKREKM---ESSPVSSLIHKFGKLSFY 1115
BLAST of Spg008554 vs. NCBI nr
Match:
KAG7032582.1 (RNA-dependent RNA polymerase 1, partial [Cucurbita argyrosperma subsp. argyrosperma])
HSP 1 Score: 1900.6 bits (4922), Expect = 0.0e+00
Identity = 944/1122 (84.14%), Postives = 1013/1122 (90.29%), Query Frame = 0
Query: 3 TIEIFGFPANVAVDDVKEFLENHTGDGTVSTVRISKPKDENSRFTFATVRFTSELAAEYV 62
TIE+FGF V+ +VKEF+ENHTG+GTVS VRISKPKD+ SR TFA V F S++AAEYV
Sbjct: 7 TIEVFGFGREVSAGEVKEFMENHTGEGTVSKVRISKPKDKKSRLTFARVEFRSKVAAEYV 66
Query: 63 VEQAEAEERLWYESSYLKARGVEREMRAAAGRRGE-VERIENVRIHLGSRISKERMSVIW 122
VE+AEAEERLW+ YLKARGVERE R E +ER+E VR+ LGS ISK+ M VIW
Sbjct: 67 VEKAEAEERLWFGRWYLKARGVEREREREREMRVERLERLEKVRVQLGSVISKDEMRVIW 126
Query: 123 RGENCCVEFGVGIRKLCFYLSY--EFDEYKMELSFENILGVELRCPLDPPSNLLLIQLQG 182
RGE+ VE+G G+RKL FYLSY E D+YKMEL FENILGV+LRCP + SNL LIQLQG
Sbjct: 127 RGEDWSVEYGNGMRKLWFYLSYGVEDDDYKMELRFENILGVQLRCPHNSSSNLFLIQLQG 186
Query: 183 APRIFRK---SSSSSSAQFYSKESAGFRWIRDVDFTPSSSIGQSFTICLEVSPEHQLPSF 242
APRIF+K SSSSSS+QFYSKES GFRWIRDVDFTPSS IGQSF +C EVSP QLPSF
Sbjct: 187 APRIFKKCPSSSSSSSSQFYSKESTGFRWIRDVDFTPSSCIGQSFALCFEVSPGDQLPSF 246
Query: 243 FETLVGYKERYGPFNLETDSSFSSTSNLVPIISPPQGFDFSYKILFKINALVQHGYLPGP 302
F+TLVGYKE YGPF L T S+FSSTSNLVPI++PPQ F YKILFKINALVQHGYLPG
Sbjct: 247 FQTLVGYKEIYGPFKLSTGSAFSSTSNLVPIVTPPQPFHIPYKILFKINALVQHGYLPGS 306
Query: 303 VLDDEFFRLVDPSRFHPDCVEQALEKLFNLKECCYKPKKWLRQQYLSFYSSKQPSWKPNI 362
LDD+FFRLVDP+RF D +E ALEKLF+LKECCY+P+KWL+QQYLSF SS Q WK NI
Sbjct: 307 ALDDKFFRLVDPTRFRSDYIEYALEKLFSLKECCYEPQKWLKQQYLSFCSSMQLPWKANI 366
Query: 363 SLDDGLVYVHRVQITPSKVYFRGPEANLSNRVVRRFIDDIDNFLRVSFVDEELDKIRSID 422
SLDD LVYVHRVQITP+KVYFRGPEANLSNRVVRRFIDDIDNFLRVSFVDEELDKI S D
Sbjct: 367 SLDDSLVYVHRVQITPAKVYFRGPEANLSNRVVRRFIDDIDNFLRVSFVDEELDKIHSSD 426
Query: 423 LSPRTSAENTERTRVYDRVLSVLRNGIVIGEKKFEFLAFSASQLRENSFWMFASREGLTA 482
LSPRTS E ERTRVYDR+LSVLRNGI+IG KKFEFLAFSASQLRENSFWMFASREGL A
Sbjct: 427 LSPRTSTEKIERTRVYDRILSVLRNGILIGGKKFEFLAFSASQLRENSFWMFASREGLNA 486
Query: 483 ADIREWMGDFRQIRNVAKYAARLGQSFGSSRKTLCVEEDEIEVIPDVEVVRKKIKYCFSD 542
ADIREWMGDFR+IRNVAKYAARLGQSFGSSRKTLCVEE+EIEVIPDVEV RK IKYCFSD
Sbjct: 487 ADIREWMGDFREIRNVAKYAARLGQSFGSSRKTLCVEENEIEVIPDVEVERKNIKYCFSD 546
Query: 543 GIGKISETLAEKVAEKCGLISHTPSAFQIRYAGYKGVVAIDPTSKKKLSLRKSMLKYMSL 602
GIGKISE LAE VAEKCGLISHTPSAFQIRYAGYKGVVA+DPTSKKKLSLRKSMLKYMSL
Sbjct: 547 GIGKISEALAEMVAEKCGLISHTPSAFQIRYAGYKGVVAVDPTSKKKLSLRKSMLKYMSL 606
Query: 603 DTQLDVLSWSKYQPCFLNRQVINLLSTLGIRDHVFVKKQKEAIDQLDSILHDPSKALEVL 662
DTQLDVLSWSKYQPCFLNRQVINLLSTLGI+D VFVK+QK+A+DQLDSIL DPSKALEVL
Sbjct: 607 DTQLDVLSWSKYQPCFLNRQVINLLSTLGIQDDVFVKQQKKAVDQLDSILRDPSKALEVL 666
Query: 663 ELMSPGEMTSILKEMLLFYKPNEEPFLNMMLRTFRADKLLDLRTKSRIFVPKGRTMMGCL 722
ELMSPGEMTS+LK+MLLFY P+EEPFLNMMLRTFRADKLLDLRTKSRIFVP+GRTMMGCL
Sbjct: 667 ELMSPGEMTSVLKDMLLFYNPHEEPFLNMMLRTFRADKLLDLRTKSRIFVPEGRTMMGCL 726
Query: 723 DETQTLEYGQVFVHCSVPGRSSENSFVVKGKVVVAKNPCLHPGDVRLLDAVDVKALHHMV 782
DET+TLEYGQVF+HCSVPGRSSE++FVVKGKVVVAKNPCLHPGDVR+LDAVDVK LHHMV
Sbjct: 727 DETRTLEYGQVFIHCSVPGRSSESNFVVKGKVVVAKNPCLHPGDVRVLDAVDVKTLHHMV 786
Query: 783 DCVVFPQKGERPHPNECSGSDLDGDLYFVCWDSKLICIRPVKPMSYEPAPTIQLDHDVTI 842
DCVVFPQKG+RPHPNECSGSDLDGDLYFVCWD +L CIRP+KPMSYEPAPTIQLDHDVTI
Sbjct: 787 DCVVFPQKGKRPHPNECSGSDLDGDLYFVCWDPELTCIRPIKPMSYEPAPTIQLDHDVTI 846
Query: 843 EEVQEYLAKYMVNDGLGGIANAHTVFADKKPKKAMSAECVELAKLFSIAVDFPKTGVPAN 902
EEVQEY A YMVNDGLGGIANAHTVFADKKPKKAMSAEC+ELAKLFSIAVDFPKTGVPAN
Sbjct: 847 EEVQEYFANYMVNDGLGGIANAHTVFADKKPKKAMSAECIELAKLFSIAVDFPKTGVPAN 906
Query: 903 LPRSLRVHEYPDFMEKPDKPTYVSNGVLGKLFRGVKDVSSDINTIEAFTREVATKCYDPD 962
LPRSLRVHEYPDFMEKP+K TY+SNGV+GKLFRGVKDVSSDIN IE FTREVA KCYDPD
Sbjct: 907 LPRSLRVHEYPDFMEKPNKRTYISNGVIGKLFRGVKDVSSDINNIETFTREVAAKCYDPD 966
Query: 963 MEVDGFENYLREAFDYKNRYDFKLGNLMDYYGIKTEPELVSGNILRMAKSFDKRNDLEQI 1022
MEV GFENYL EAFDYK+ YDFKLGNLMDYYGI+TEPELVSGNILRM KSFDKRND+EQI
Sbjct: 967 MEVMGFENYLSEAFDYKSMYDFKLGNLMDYYGIETEPELVSGNILRMGKSFDKRNDMEQI 1026
Query: 1023 SLAMKSLRKEARAWFNEKGSKSTY----DEDEEYAKASAWYHVTYHPNYWGRYNKGMQRD 1082
SLAM+SLRKEAR WFNEKGSKS Y DED+EYAKASAWYHVTYHPN+WGRYN+GMQRD
Sbjct: 1027 SLAMRSLRKEARGWFNEKGSKSIYNNNKDEDDEYAKASAWYHVTYHPNFWGRYNEGMQRD 1086
Query: 1083 HFLSFPWCVSDQLIQIKREKMCQISYSQVSSLIHKFGDLSFY 1115
HFLSFPWCVS++LIQIKREKM S VSSLIHKFG LSFY
Sbjct: 1087 HFLSFPWCVSEKLIQIKREKM---ESSPVSSLIHKFGKLSFY 1125
BLAST of Spg008554 vs. NCBI nr
Match:
XP_023524039.1 (probable RNA-dependent RNA polymerase 1 isoform X1 [Cucurbita pepo subsp. pepo])
HSP 1 Score: 1892.1 bits (4900), Expect = 0.0e+00
Identity = 935/1116 (83.78%), Postives = 1003/1116 (89.87%), Query Frame = 0
Query: 3 TIEIFGFPANVAVDDVKEFLENHTGDGTVSTVRISKPKDENSRFTFATVRFTSELAAEYV 62
TIE+FGF V+ +VKEF+ENHTG+GTVS VRIS+PKDE +R T+A V F S++AAEYV
Sbjct: 7 TIEVFGFGREVSAGEVKEFMENHTGEGTVSKVRISEPKDEKARLTYARVEFRSKVAAEYV 66
Query: 63 VEQAEAEERLWYESSYLKARGVEREMRAAAGRRGEVERIENVRIHLGSRISKERMSVIWR 122
VE+AEAEERLW+ YLKARGVER R VER+E VR+ LGS + KE M VIWR
Sbjct: 67 VEKAEAEERLWFGRWYLKARGVER----GREREMRVERMEKVRVQLGSVVWKEEMRVIWR 126
Query: 123 GENCCVEFGVGIRKLCFYLSYEFDEYKMELSFENILGVELRCPLDPPSNLLLIQLQGAPR 182
GEN VE+G G+RKL FYLSY +YKMEL FENILGV+LRCP + SNL LIQLQGAPR
Sbjct: 127 GENWSVEYGNGMRKLWFYLSYGVIDYKMELRFENILGVQLRCPHNSSSNLFLIQLQGAPR 186
Query: 183 IFRKSSSSSSAQFYSKESAGFRWIRDVDFTPSSSIGQSFTICLEVSPEHQLPSFFETLVG 242
IF+K SSSS+QFYSKES GFRWIRDVDFTPSS IGQSF +C EVSP QLPSFF+TLVG
Sbjct: 187 IFKKCPSSSSSQFYSKESTGFRWIRDVDFTPSSCIGQSFALCFEVSPGDQLPSFFQTLVG 246
Query: 243 YKERYGPFNLETDSSFSSTSNLVPIISPPQGFDFSYKILFKINALVQHGYLPGPVLDDEF 302
YKE YGPF L T S+FSS SNLVPI++PPQ FD YKILFKINALVQHGYLPG LDD+F
Sbjct: 247 YKEIYGPFKLSTGSAFSSNSNLVPIVTPPQPFDIPYKILFKINALVQHGYLPGSALDDKF 306
Query: 303 FRLVDPSRFHPDCVEQALEKLFNLKECCYKPKKWLRQQYLSFYSSKQPSWKPNISLDDGL 362
FRLVDPSRF D +E ALEKLF+LKECCY+P+KWL+QQYLSF SS Q WK NISLDD L
Sbjct: 307 FRLVDPSRFRSDYIEYALEKLFSLKECCYEPQKWLKQQYLSFCSSMQLPWKANISLDDSL 366
Query: 363 VYVHRVQITPSKVYFRGPEANLSNRVVRRFIDDIDNFLRVSFVDEELDKIRSIDLSPRTS 422
VYVHRVQITP++VYFRGPEANLSNRVVRRFIDDIDNFLRVSFVDEELDKI S DLSPRTS
Sbjct: 367 VYVHRVQITPARVYFRGPEANLSNRVVRRFIDDIDNFLRVSFVDEELDKIHSSDLSPRTS 426
Query: 423 AENTERTRVYDRVLSVLRNGIVIGEKKFEFLAFSASQLRENSFWMFASREGLTAADIREW 482
E ERT VYDR+LSVLRNGIVIG KKFEFLAFSASQLRENSFWMFA REGL AADIREW
Sbjct: 427 TEKIERTSVYDRILSVLRNGIVIGGKKFEFLAFSASQLRENSFWMFAPREGLNAADIREW 486
Query: 483 MGDFRQIRNVAKYAARLGQSFGSSRKTLCVEEDEIEVIPDVEVVRKKIKYCFSDGIGKIS 542
MGDFR+IRNVAKYAARLGQSFGSSRKTLCVEE+EIEVIPDVEV RK IKYCFSDGIGKIS
Sbjct: 487 MGDFREIRNVAKYAARLGQSFGSSRKTLCVEENEIEVIPDVEVERKNIKYCFSDGIGKIS 546
Query: 543 ETLAEKVAEKCGLISHTPSAFQIRYAGYKGVVAIDPTSKKKLSLRKSMLKYMSLDTQLDV 602
E LAE VAEKCGLISHTPSAFQIRYAGYKGVVA+DPTSKKKLSLRKSMLKYMSLDTQLDV
Sbjct: 547 EALAEMVAEKCGLISHTPSAFQIRYAGYKGVVAVDPTSKKKLSLRKSMLKYMSLDTQLDV 606
Query: 603 LSWSKYQPCFLNRQVINLLSTLGIRDHVFVKKQKEAIDQLDSILHDPSKALEVLELMSPG 662
LSWSKYQPCFLNRQVINLLSTLGIRD VFV +QK+A+DQLDSIL DPSKALEVLELMSPG
Sbjct: 607 LSWSKYQPCFLNRQVINLLSTLGIRDDVFVNQQKKAVDQLDSILRDPSKALEVLELMSPG 666
Query: 663 EMTSILKEMLLFYKPNEEPFLNMMLRTFRADKLLDLRTKSRIFVPKGRTMMGCLDETQTL 722
EMTSILK+MLLFY P+EEPFLNMMLRTFRADKLLDLRTKSRIFVPKGRTMMGCLDET++L
Sbjct: 667 EMTSILKDMLLFYNPHEEPFLNMMLRTFRADKLLDLRTKSRIFVPKGRTMMGCLDETRSL 726
Query: 723 EYGQVFVHCSVPGRSSENSFVVKGKVVVAKNPCLHPGDVRLLDAVDVKALHHMVDCVVFP 782
EYGQVF+HCSVPGRSSE++FVVKGKVVVAKNPCLHPGDVR+LDAVDVK LHHMVDCVVFP
Sbjct: 727 EYGQVFIHCSVPGRSSESNFVVKGKVVVAKNPCLHPGDVRVLDAVDVKTLHHMVDCVVFP 786
Query: 783 QKGERPHPNECSGSDLDGDLYFVCWDSKLICIRPVKPMSYEPAPTIQLDHDVTIEEVQEY 842
QKG+RPHPNECSGSDLDGDLYFVCWD +L CIRP+KPMSYEPAPTIQLDHDVTIEEVQEY
Sbjct: 787 QKGKRPHPNECSGSDLDGDLYFVCWDPELTCIRPIKPMSYEPAPTIQLDHDVTIEEVQEY 846
Query: 843 LAKYMVNDGLGGIANAHTVFADKKPKKAMSAECVELAKLFSIAVDFPKTGVPANLPRSLR 902
A YMVNDGLGGIANAHTVFADKKPKKAMSAEC+ELAKLFSIAVDFPKTGVPANLPRSLR
Sbjct: 847 FANYMVNDGLGGIANAHTVFADKKPKKAMSAECIELAKLFSIAVDFPKTGVPANLPRSLR 906
Query: 903 VHEYPDFMEKPDKPTYVSNGVLGKLFRGVKDVSSDINTIEAFTREVATKCYDPDMEVDGF 962
VH+YPDFMEKP+K +Y+SNGV+GKLFRGVKDVSSDIN IE FTREVA KCYDPDMEV GF
Sbjct: 907 VHQYPDFMEKPNKRSYISNGVIGKLFRGVKDVSSDINNIETFTREVAAKCYDPDMEVMGF 966
Query: 963 ENYLREAFDYKNRYDFKLGNLMDYYGIKTEPELVSGNILRMAKSFDKRNDLEQISLAMKS 1022
ENYL EAFDYK+ YDFKLGNLMDYYGI+TEPELVSGNILRM KSFDKRND+EQISLAM+S
Sbjct: 967 ENYLSEAFDYKSMYDFKLGNLMDYYGIETEPELVSGNILRMGKSFDKRNDMEQISLAMRS 1026
Query: 1023 LRKEARAWFNEKGSKSTY----DEDEEYAKASAWYHVTYHPNYWGRYNKGMQRDHFLSFP 1082
LRKEAR WFNEKGSKS Y DED+EYAKASAWYHVTYHPN+WGRYN+GMQRDHFLSFP
Sbjct: 1027 LRKEARGWFNEKGSKSIYNNNKDEDDEYAKASAWYHVTYHPNFWGRYNEGMQRDHFLSFP 1086
Query: 1083 WCVSDQLIQIKREKMCQISYSQVSSLIHKFGDLSFY 1115
WCVS++LIQIKREKM S VSSLIHKFG LSFY
Sbjct: 1087 WCVSEKLIQIKREKM---ESSPVSSLIHKFGKLSFY 1115
BLAST of Spg008554 vs. NCBI nr
Match:
KAG6601883.1 (RNA-dependent RNA polymerase 1, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 1892.1 bits (4900), Expect = 0.0e+00
Identity = 943/1121 (84.12%), Postives = 1010/1121 (90.10%), Query Frame = 0
Query: 3 TIEIFGFPANVAVDDVKEFLENHTGDGTVSTVRISKPKDENSRFTFATVRFTSELAAEYV 62
TIE+FGF V+ +VKEF+ENHTG+GTVS VRISK KD+ SR FA V F S++AAEYV
Sbjct: 7 TIEVFGFGREVSAGEVKEFMENHTGEGTVSKVRISKRKDKKSR--FARVEFRSKVAAEYV 66
Query: 63 VEQAEAEERLWYESSYLKARGVEREMRAAAGRRGEVERIENVRIHLGSRISKERMSVIWR 122
VE+AEAEERLW+ YLKARGVEREMR VER+E VR+ LGS ISK+ M VIWR
Sbjct: 67 VEKAEAEERLWFGRWYLKARGVEREMR--------VERLEKVRVQLGSVISKDEMRVIWR 126
Query: 123 GENCCVEFGVGIRKLCFYLSY--EFDEYKMELSFENILGVELRCPLDPPSNLLLIQLQGA 182
GE+ VE+G G+RKL FYLSY E D+YKMEL FENILGV+LRCP + SNL LIQLQGA
Sbjct: 127 GEDWSVEYGNGMRKLWFYLSYGVEDDDYKMELRFENILGVQLRCPHNSSSNLFLIQLQGA 186
Query: 183 PRIFRK---SSSSSSAQFYSKESAGFRWIRDVDFTPSSSIGQSFTICLEVSPEHQLPSFF 242
PRIF+K SSSSSS+QFYSKES GFRWIRDVDFTPSS IGQSF +C EVSP QLPSFF
Sbjct: 187 PRIFKKCPSSSSSSSSQFYSKESTGFRWIRDVDFTPSSCIGQSFALCFEVSPGDQLPSFF 246
Query: 243 ETLVGYKERYGPFNLETDSSFSSTSNLVPIISPPQGFDFSYKILFKINALVQHGYLPGPV 302
+TLVGYKE YGPF L T S+FSSTSNLVPI++PPQ F YKILFKINALVQHGYLPG
Sbjct: 247 QTLVGYKEIYGPFKLSTGSAFSSTSNLVPIVTPPQPFHIPYKILFKINALVQHGYLPGSA 306
Query: 303 LDDEFFRLVDPSRFHPDCVEQALEKLFNLKECCYKPKKWLRQQYLSFYSSKQPSWKPNIS 362
LDD+FFRLVDP+RF D +E ALEKLF+LKECCY+P+KWL+QQYLSF SS Q WK NIS
Sbjct: 307 LDDKFFRLVDPTRFRSDYIEYALEKLFSLKECCYEPQKWLKQQYLSFCSSMQLPWKANIS 366
Query: 363 LDDGLVYVHRVQITPSKVYFRGPEANLSNRVVRRFIDDIDNFLRVSFVDEELDKIRSIDL 422
LDD LVYVHRVQITP+KVYFRGPEANLSNRVVRRFIDDIDNFLRVSFVDEELDKI S DL
Sbjct: 367 LDDSLVYVHRVQITPAKVYFRGPEANLSNRVVRRFIDDIDNFLRVSFVDEELDKIHSSDL 426
Query: 423 SPRTSAENTERTRVYDRVLSVLRNGIVIGEKKFEFLAFSASQLRENSFWMFASREGLTAA 482
SPRTS E ERTRVYDR+LSVLRNGI+IG KKFEFLAFSASQLRENSFWMFASREGL AA
Sbjct: 427 SPRTSTEKIERTRVYDRILSVLRNGILIGGKKFEFLAFSASQLRENSFWMFASREGLNAA 486
Query: 483 DIREWMGDFRQIRNVAKYAARLGQSFGSSRKTLCVEEDEIEVIPDVEVVRKKIKYCFSDG 542
DIREWMGDFR+IRNVAKYAARLGQSFGSSRKTLCVEE+EIEVIPDVEV RK IKYCFSDG
Sbjct: 487 DIREWMGDFREIRNVAKYAARLGQSFGSSRKTLCVEENEIEVIPDVEVERKNIKYCFSDG 546
Query: 543 IGKISETLAEKVAEKCGLISHTPSAFQIRYAGYKGVVAIDPTSKKKLSLRKSMLKYMSLD 602
IGKISE LAE VAEKCGLISHTPSAFQIRYAGYKGVVA+DPTSKKKLSLRKSMLKYMSLD
Sbjct: 547 IGKISEALAEMVAEKCGLISHTPSAFQIRYAGYKGVVAVDPTSKKKLSLRKSMLKYMSLD 606
Query: 603 TQLDVLSWSKYQPCFLNRQVINLLSTLGIRDHVFVKKQKEAIDQLDSILHDPSKALEVLE 662
TQLDVLSWSKYQPCFLNRQVINLLSTLGI+D VFVK+QK+A+DQLDSIL DPSKALEVLE
Sbjct: 607 TQLDVLSWSKYQPCFLNRQVINLLSTLGIQDDVFVKQQKKAVDQLDSILRDPSKALEVLE 666
Query: 663 LMSPGEMTSILKEMLLFYKPNEEPFLNMMLRTFRADKLLDLRTKSRIFVPKGRTMMGCLD 722
LMSPGEMTS+LK+MLLFY P+EEPFLNMMLRTFRADKLLDLRTKSRIFVP+GRTMMGCLD
Sbjct: 667 LMSPGEMTSVLKDMLLFYNPHEEPFLNMMLRTFRADKLLDLRTKSRIFVPEGRTMMGCLD 726
Query: 723 ETQTLEYGQVFVHCSVPGRSSENSFVVKGKVVVAKNPCLHPGDVRLLDAVDVKALHHMVD 782
ET+TLEYGQVF+HCSVPGRSSE++FVVKGKVVVAKNPCLHPGDVR+LDAVDVK LHHMVD
Sbjct: 727 ETRTLEYGQVFIHCSVPGRSSESNFVVKGKVVVAKNPCLHPGDVRVLDAVDVKTLHHMVD 786
Query: 783 CVVFPQKGERPHPNECSGSDLDGDLYFVCWDSKLICIRPVKPMSYEPAPTIQLDHDVTIE 842
CVVFPQKG+RPHPNECSGSDLDGDLYFVCWD +L CIRP+KPMSYEPAPTIQLDHDVTIE
Sbjct: 787 CVVFPQKGKRPHPNECSGSDLDGDLYFVCWDPELTCIRPIKPMSYEPAPTIQLDHDVTIE 846
Query: 843 EVQEYLAKYMVNDGLGGIANAHTVFADKKPKKAMSAECVELAKLFSIAVDFPKTGVPANL 902
EVQEY A YMVNDGLGGIANAHTVFADKKPKKAMSAEC+ELAKLFSIAVDFPKTGVPANL
Sbjct: 847 EVQEYFANYMVNDGLGGIANAHTVFADKKPKKAMSAECIELAKLFSIAVDFPKTGVPANL 906
Query: 903 PRSLRVHEYPDFMEKPDKPTYVSNGVLGKLFRGVKDVSSDINTIEAFTREVATKCYDPDM 962
PRSLRVHEYPDFMEKP+K TY SNGV+GKLFRGVKDVSSDIN IE FTREVA KCYDPDM
Sbjct: 907 PRSLRVHEYPDFMEKPNKRTYTSNGVIGKLFRGVKDVSSDINNIETFTREVAAKCYDPDM 966
Query: 963 EVDGFENYLREAFDYKNRYDFKLGNLMDYYGIKTEPELVSGNILRMAKSFDKRNDLEQIS 1022
EV GFENYL EAFDYK+ YDFKLGNLMDYYGI+TEPELVSGNILRM KSFDKRND+EQIS
Sbjct: 967 EVMGFENYLSEAFDYKSMYDFKLGNLMDYYGIETEPELVSGNILRMGKSFDKRNDMEQIS 1026
Query: 1023 LAMKSLRKEARAWFNEKGSKSTY----DEDEEYAKASAWYHVTYHPNYWGRYNKGMQRDH 1082
LAM+SLRKEAR WFNEKGSKS Y DED+EYAKASAWYHVTYHPN+WGRYN+GMQRDH
Sbjct: 1027 LAMRSLRKEARGWFNEKGSKSIYNNNKDEDDEYAKASAWYHVTYHPNFWGRYNEGMQRDH 1086
Query: 1083 FLSFPWCVSDQLIQIKREKMCQISYSQVSSLIHKFGDLSFY 1115
FLSFPWCVS++LIQIKREKM S VSSLIHKFG LSFY
Sbjct: 1087 FLSFPWCVSEKLIQIKREKM---ESSPVSSLIHKFGKLSFY 1114
BLAST of Spg008554 vs. ExPASy Swiss-Prot
Match:
Q9LQV2 (RNA-dependent RNA polymerase 1 OS=Arabidopsis thaliana OX=3702 GN=RDR1 PE=2 SV=1)
HSP 1 Score: 1255.4 bits (3247), Expect = 0.0e+00
Identity = 635/1098 (57.83%), Postives = 805/1098 (73.32%), Query Frame = 0
Query: 3 TIEIFGFPANVAVDDVKEFLENHTGDGTVSTVRISKPKDENSRFTFATVRFTSELAAEYV 62
TI++FGFP V+ ++VK+FLE TG GTV +++ +PK R +A V+FTSE +
Sbjct: 4 TIQVFGFPNGVSAEEVKKFLERLTGSGTVYAIKVRQPKKGGPR-VYAIVQFTSERHTRLI 63
Query: 63 VEQAEAEERLWYESSYLKARGVEREMRAAAGRRGEVERIENVRIHLGSRISKERMSVIWR 122
+ A ERL+Y SYLKA VE+++ R + I +++ G ++S ++ +W
Sbjct: 64 I--TAAAERLYYGRSYLKAFEVEQDI--VPKPRASLHTISGLKMFFGCQVSTKKFLTLWS 123
Query: 123 GENCCVEFGVGIRKLCFYLSYEFDEYKMELSFENILGVELRCPLDPPSNLLLIQLQGAPR 182
++ CV FG+G+RKL F S+ +Y++ELS+ENI ++L P S L+IQ+ GAP+
Sbjct: 124 AQDVCVSFGIGMRKLHFSFSWYQKDYRLELSYENIWQIDLHSPQGRSSKFLVIQVIGAPK 183
Query: 183 IFRKSSSSSSAQF----YSKESAGFRWIRDVDFTPSSSIGQSFTICLEVSPEHQLPSFFE 242
IF K + F + + + +WIR DFT SS IGQS CLE+ +P F E
Sbjct: 184 IFEKEDQPINLLFGIMDFYSDGSDEQWIRTTDFTSSSCIGQSTAFCLELPVHLNVPDFRE 243
Query: 243 TLVGYKE-RYGPFNLETDSSFSSTSN-LVPIISPPQGFDFSYKILFKINALVQHGYLPGP 302
Y E R F +E+ SS+SS +N LVP++ PP GF ++ILFK+N LVQ+ L GP
Sbjct: 244 NFANYAEHRASSFLIESGSSYSSNANTLVPVVDPPPGFSLPFEILFKLNTLVQNACLSGP 303
Query: 303 VLDDEFFRLVDPSRFHPDCVEQALEKLFNLKECCYKPKKWLRQQYLSFYSSKQPSWKPNI 362
LD +F+RL++ ++ ++ LEKLF+L ECCY+P WLR +Y + S + P I
Sbjct: 304 ALDLDFYRLLNQKKYDRALIDHCLEKLFHLGECCYEPAHWLRDEYKKWISKGKLPLSPTI 363
Query: 363 SLDDGLVYVHRVQITPSKVYFRGPEANLSNRVVRRFIDDIDNFLRVSFVDEELDKIRSID 422
SLDDGLVY++RVQ+TP++VYF GPE N+SNRV+R + I+NFLRVSFVDE+L+K+RS+D
Sbjct: 364 SLDDGLVYMYRVQVTPARVYFSGPEVNVSNRVLRHYSKYINNFLRVSFVDEDLEKVRSMD 423
Query: 423 LSPRTSAENTERTRVYDRVLSVLRNGIVIGEKKFEFLAFSASQLRENSFWMFASREGLTA 482
LSPR+S + RT++YDR+ SVLR+GIVIG+KKFEFLAFS+SQLRENS WMFA + +TA
Sbjct: 424 LSPRSSTQ--RRTKLYDRIYSVLRDGIVIGDKKFEFLAFSSSQLRENSAWMFAPIDRITA 483
Query: 483 ADIREWMGDFRQIRNVAKYAARLGQSFGSSRKTLCVEEDEIEVIPDVEVVRKKIKYCFSD 542
A IR WMGDF IRNVAKYAARLGQSF SSR+TL V DEIEVIPDVE++ +Y FSD
Sbjct: 484 AHIRAWMGDFDHIRNVAKYAARLGQSFSSSRETLNVRSDEIEVIPDVEIISLGTRYVFSD 543
Query: 543 GIGKISETLAEKVAEKCGLISHTPSAFQIRYAGYKGVVAIDPTSKKKLSLRKSMLKYMSL 602
GIGKIS A KVA KCGL +PSAFQIRY GYKGVVA+DP S KKLSLRKSM K+ S
Sbjct: 544 GIGKISAEFARKVARKCGLTEFSPSAFQIRYGGYKGVVAVDPNSSKKLSLRKSMSKFESE 603
Query: 603 DTQLDVLSWSKYQPCFLNRQVINLLSTLGIRDHVFVKKQKEAIDQLDSILHDPSKALEVL 662
+T+LDVL+WSKYQPC++NRQ+I LLSTLG+ D VF KKQ+E +D+LD+IL P +A E L
Sbjct: 604 NTKLDVLAWSKYQPCYMNRQLITLLSTLGVTDSVFEKKQREVVDRLDAILTHPLEAHEAL 663
Query: 663 ELMSPGEMTSILKEMLLF-YKPNEEPFLNMMLRTFRADKLLDLRTKSRIFVPKGRTMMGC 722
LM+PGE T+ILK ++L YKP+ EPFL+MML+ FRA KLL+LRTK+RIF+ GR+MMGC
Sbjct: 664 GLMAPGENTNILKALILCGYKPDAEPFLSMMLQNFRASKLLELRTKTRIFISGGRSMMGC 723
Query: 723 LDETQTLEYGQVFVHCSVPGRSSENSFVVKGKVVVAKNPCLHPGDVRLLDAVDVKALHHM 782
LDET+TLEYGQV V S P R F++ G VVVAKNPCLHPGDVR+L AV+V AL+HM
Sbjct: 724 LDETRTLEYGQVVVQYSDPMRPGRR-FIITGPVVVAKNPCLHPGDVRVLQAVNVPALNHM 783
Query: 783 VDCVVFPQKGERPHPNECSGSDLDGDLYFVCWDSKLICIRPVKPMSYEPAPTIQLDHDVT 842
VDCVVFPQKG RPHPNECSGSDLDGD+YFVCWD +L+ R +PM Y P PT LDHDVT
Sbjct: 784 VDCVVFPQKGLRPHPNECSGSDLDGDIYFVCWDQELVPPRTSEPMDYTPEPTQILDHDVT 843
Query: 843 IEEVQEYLAKYMVNDGLGGIANAHTVFADKKPKKAMSAECVELAKLFSIAVDFPKTGVPA 902
IEEV+EY A Y+VND LG IANAHT FADK+P KA S C+ELAK FS AVDFPKTGV A
Sbjct: 844 IEEVEEYFANYIVNDSLGIIANAHTAFADKEPLKAFSDPCIELAKKFSTAVDFPKTGVAA 903
Query: 903 NLPRSLRVHEYPDFMEKPDKPTYVSNGVLGKLFRGVKDVSSDINTIEAFTREVATKCYDP 962
+P+ L V EYPDFMEKPDKPTY S V+GKLFR VK+ + + +I++FT +VA+K YD
Sbjct: 904 VIPQHLYVKEYPDFMEKPDKPTYESKNVIGKLFREVKERAPPLISIKSFTLDVASKSYDK 963
Query: 963 DMEVDGFENYLREAFDYKNRYDFKLGNLMDYYGIKTEPELVSGNILRMAKSFDKRNDLEQ 1022
DMEVDGFE Y+ EAF K YDFKLGNLMDYYGIKTE E++SG I+RM+KSF KR D E
Sbjct: 964 DMEVDGFEEYVDEAFYQKANYDFKLGNLMDYYGIKTEAEILSGGIMRMSKSFTKRRDAES 1023
Query: 1023 ISLAMKSLRKEARAWFNEKGSKSTYDEDEEYAKASAWYHVTYHPNYWGRYNKGMQRDHFL 1082
I A+++LRKE + FN ++ +E+ E AKASAWYHVTYH +YWG YN+G+ RDHFL
Sbjct: 1024 IGRAVRALRKETLSLFN-----ASEEEENESAKASAWYHVTYHSSYWGLYNEGLNRDHFL 1083
Query: 1083 SFPWCVSDQLIQIKREKM 1094
SF WCV D+L++IK+ +
Sbjct: 1084 SFAWCVYDKLVRIKKTNL 1088
BLAST of Spg008554 vs. ExPASy Swiss-Prot
Match:
Q0DXS3 (Probable RNA-dependent RNA polymerase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=RDR1 PE=2 SV=2)
HSP 1 Score: 916.8 bits (2368), Expect = 2.4e-265
Identity = 456/723 (63.07%), Postives = 567/723 (78.42%), Query Frame = 0
Query: 373 SKVYFRGPEANLSNRVVRRFIDDIDNFLRVSFVDEELDKIRSIDLSPRT-SAENTERTRV 432
S VYF GPE N+SNRVVR F DI+NFLR+SFVDE+ +K+R+ DLSPR+ S + RT +
Sbjct: 7 STVYFYGPEINVSNRVVRNFSSDIENFLRISFVDEDCEKLRATDLSPRSASGHDANRTAL 66
Query: 433 YDRVLSVLRNGIVIGEKKFEFLAFSASQLRENSFWMFASREGLTAADIREWMGDFRQIRN 492
Y RVLSVL +GI IG K FEFLAFS+SQLR+NS WMFASR+GL A+DIR WMGDFR IRN
Sbjct: 67 YKRVLSVLSDGITIGGKNFEFLAFSSSQLRDNSAWMFASRQGLAASDIRTWMGDFRNIRN 126
Query: 493 VAKYAARLGQSFGSSRKTLCVEEDEIEVIPDVEVVRKKIKYCFSDGIGKISETLAEKVAE 552
VAKYAARLGQSF SS +TL V++ E+E I D ++ ++ FSDGIGKIS A +VA
Sbjct: 127 VAKYAARLGQSFSSSTETLKVQKYEVEEISD---IKNGTQHVFSDGIGKISSAFANEVAM 186
Query: 553 KCGLISHTPSAFQIRYAGYKGVVAIDPTSKKKLSLRKSMLKYMSLDTQLDVLSWSKYQPC 612
KC L PSAFQIRY GYKGVVA+DPTS+ KLSLRKSMLK+ S + +DVL++SKYQP
Sbjct: 187 KCNLKRFAPSAFQIRYGGYKGVVAVDPTSRWKLSLRKSMLKFQSDNITVDVLAYSKYQPG 246
Query: 613 FLNRQVINLLSTLGIRDHVFVKKQKEAIDQLDSILHDPSKALEVLELMSPGEMTSILKEM 672
FLNRQ+I LLSTLG+RD VF +KQ+EA++QL+ ++ DP A+E +ELM GE+T+ +KE+
Sbjct: 247 FLNRQLITLLSTLGVRDSVFEQKQEEAVNQLNKMVTDPQAAIEAIELMPMGEITNAVKEL 306
Query: 673 LLF-YKPNEEPFLNMMLRTFRADKLLDLRTKSRIFVPKGRTMMGCLDETQTLEYGQVFVH 732
LL Y+P++EP+L+M+L+TFRA KLL+L+TKSRI +PKGR MMGCLDET+TL+YGQVF+
Sbjct: 307 LLCGYQPDDEPYLSMLLQTFRASKLLELKTKSRILIPKGRAMMGCLDETRTLKYGQVFIR 366
Query: 733 CSVPGRSSENSFVVKGKVVVAKNPCLHPGDVRLLDAVDVKALHHMVDCVVFPQKGERPHP 792
+ G + + F V GKVV+AKNPCLHPGD+R+L AVDV LHHM +CVVFPQ+G RPHP
Sbjct: 367 -ATSGVNDNDRFTVTGKVVIAKNPCLHPGDIRILHAVDVPVLHHMFNCVVFPQQGPRPHP 426
Query: 793 NECSGSDLDGDLYFVCWDSKLICIRPVKPMSYEPAPTIQLDHDVTIEEVQEYLAKYMVND 852
NECSGSDLDGD+YFV WD LI R V PM Y PAPT LDHDVTIEEV+EY Y+VN+
Sbjct: 427 NECSGSDLDGDIYFVSWDPSLIPPRMVTPMDYTPAPTETLDHDVTIEEVEEYFTNYIVNE 486
Query: 853 GLGGIANAHTVFADKKPKKAMSAECVELAKLFSIAVDFPKTGVPANLPRSLRVHEYPDFM 912
LG IANAH VFADK+ KA S+ C+ELAKLFSIAVDFPKTGVPA +P L V EYPDFM
Sbjct: 487 SLGMIANAHVVFADKEDLKAESSPCIELAKLFSIAVDFPKTGVPALIPPELHVKEYPDFM 546
Query: 913 EKPDKPTYVSNGVLGKLFRGVKDVSSDINTIEAFTREVATKCYDPDMEVDGFENYLREAF 972
EK DK TY S GV+GKL+R +K + I+ FTREVA + YD DM VDG+E+Y+ EA
Sbjct: 547 EKLDKVTYESKGVIGKLYREIKKHTPH---IKHFTREVARRSYDTDMIVDGYEDYITEAM 606
Query: 973 DYKNRYDFKLGNLMDYYGIKTEPELVSGNILRMAKSFDKRNDLEQISLAMKSLRKEARAW 1032
K+ YDFKLGNLMD+YGIK+E E++SG IL+MAK+F K++D + I LA++SLRKEAR+
Sbjct: 607 ALKDEYDFKLGNLMDHYGIKSEAEIISGCILKMAKNFTKKSDADAIRLAVRSLRKEARSR 666
Query: 1033 FNEKG-SKSTYDEDEEYAKASAWYHVTYHPNYWGRYNKGMQRDHFLSFPWCVSDQLIQIK 1092
F+E + + D AKASAWYHVTYHP +WG YN+G +R HF+SFPWC+ ++L++IK
Sbjct: 667 FSEMSLDDNGHGHDASEAKASAWYHVTYHPEFWGCYNEGYERPHFISFPWCIYEKLLRIK 722
BLAST of Spg008554 vs. ExPASy Swiss-Prot
Match:
O82504 (RNA-dependent RNA polymerase 2 OS=Arabidopsis thaliana OX=3702 GN=RDR2 PE=1 SV=1)
HSP 1 Score: 765.0 bits (1974), Expect = 1.2e-219
Identity = 451/1142 (39.49%), Postives = 663/1142 (58.06%), Query Frame = 0
Query: 2 ATIEIFGFPANVAVDDVKEFLENHTGDGTVSTVRISKPKDENSRFTFATVRFTSELAAEY 61
+T++I P + D++ FLE H G+ TV + I +D FA V+FT+ L +
Sbjct: 10 STVKISNVPQTIVADELLRFLELHLGEDTVFALEIPTTRDNWKPRDFARVQFTT-LEVKS 69
Query: 62 VVEQAEAEERLWYESSYLKARGVEREM--RAAAGRRGEVERIENVRIHLGSRISKERMSV 121
+ ++ +L +++ L+ ++ R R+ R++++ + +G S E+
Sbjct: 70 RAQLLSSQSKLLFKTHNLRLSEAYDDIIPRPVDPRK----RLDDIVLTVGFPESDEKRFC 129
Query: 122 I---WRGENCCVEFGVGIRKLCFYLSYEFDEYKMELSFENILGVELRCPLDPPSNL--LL 181
W G C + R++ F++ D YK+E+ FE+I+ C S + L
Sbjct: 130 ALEKWDGVRCWIL--TEKRRVEFWVWESGDCYKIEVRFEDIIETLSCCVNGDASEIDAFL 189
Query: 182 IQLQGAPRIFRKSSSSSSAQF------YSKESAGFRWIRDVDFTPSSSIGQSFTICLEVS 241
++L+ P++F++ + + +F + KE F WIR DF+ S SIG S CLEV
Sbjct: 190 LKLKYGPKVFKRVTVHIATKFKSDRYRFCKEDFDFMWIRTTDFSGSKSIGTSTCFCLEVH 249
Query: 242 PEHQLPSFFETLVGYKERYGPFNLETDSSFSSTSNLVPIISPP-QGFDFSYKILFKINAL 301
+ F L Y+E +F+S + +VP+++ G +F Y+ILF++NAL
Sbjct: 250 NGSTMLDIFSGLPYYREDTLSLTYVDGKTFASAAQIVPLLNAAILGLEFPYEILFQLNAL 309
Query: 302 VQHGYLP-GPVLDDEFFRLVDPSRFHPDCVEQALEKLFNLKECCYKPKKWLRQQYLSFYS 361
V + D E +++ V L+KL CY P +++ Q S
Sbjct: 310 VHAQKISLFAASDMELIKILRGMSLETALV--ILKKLHQQSSICYDPVFFVKTQMQSVVK 369
Query: 362 SKQ--PSWKPNISLDDGLVYVHRVQITPSKVYFRGPEANLSNRVVRRFIDDIDNFLRVSF 421
+ P+ + ++ R +TPSK+Y GPE +N VV+ F + + +F+RV+F
Sbjct: 370 KMKHSPASAYKRLTEQNIMSCQRAYVTPSKIYLLGPELETANYVVKNFAEHVSDFMRVTF 429
Query: 422 VDEELDKIRSIDLSPRTSA---ENTERTRVYDRVLSVLRNGIVIGEKKFEFLAFSASQLR 481
V+E+ K+ + LS + RT +Y+RVLS+L GI +G K+FEFLAFSASQLR
Sbjct: 430 VEEDWSKLPANALSVNSKEGYFVKPSRTNIYNRVLSILGEGITVGPKRFEFLAFSASQLR 489
Query: 482 ENSFWMFASREGLTAADIREWMGDFRQIRNVAKYAARLGQSFGSSRKTLCVEEDEIEVIP 541
NS WMFAS E + A DIREWMG FR+IR+++K AAR+GQ F +SR+TL V ++E IP
Sbjct: 490 GNSVWMFASNEKVKAEDIREWMGCFRKIRSISKCAARMGQLFSASRQTLIVRAQDVEQIP 549
Query: 542 DVEVVRKKIKYCFSDGIGKISETLAEKVAEKCGLISHTPSAFQIRYAGYKGVVAIDPTSK 601
D+EV YCFSDGIGKIS A++VA+KCGL SH PSAFQIRY GYKGV+A+D +S
Sbjct: 550 DIEVTTDGADYCFSDGIGKISLAFAKQVAQKCGL-SHVPSAFQIRYGGYKGVIAVDRSSF 609
Query: 602 KKLSLRKSMLKYMSLDTQLDVLSWSKYQPCFLNRQVINLLSTLGIRDHVFVKKQKEAIDQ 661
+KLSLR SMLK+ S + L+V W++ PCFLNR++I LLSTLGI D +F Q +
Sbjct: 610 RKLSLRDSMLKFDSNNRMLNVTRWTESMPCFLNREIICLLSTLGIEDAMFEAMQAVHLSM 669
Query: 662 LDSILHDPSKALEVLELMSPGEMTSILKEMLL-FYKPNEEPFLNMMLRTFRADKLLDLRT 721
L ++L D AL VL+ +S ++L +MLL Y P+ EP+L+MMLR +L +L++
Sbjct: 670 LGNMLEDRDAALNVLQKLSGENSKNLLVKMLLQGYAPSSEPYLSMMLRVHHESQLSELKS 729
Query: 722 KSRIFVPKGRTMMGCLDETQTLEYGQVFVHCSVP------------GRSSENSFVVKGKV 781
+ RI VPKGR ++GC+DE LEYGQV+V ++ + E + VV GKV
Sbjct: 730 RCRILVPKGRILIGCMDEMGILEYGQVYVRVTLTKAELKSRDQSYFRKIDEETSVVIGKV 789
Query: 782 VVAKNPCLHPGDVRLLDAVDVKALHH--MVDCVVFPQKGERPHPNECSGSDLDGDLYFVC 841
VV KNPCLHPGD+R+LDA+ +DC++FPQKGERPHPNECSG DLDGD +FV
Sbjct: 790 VVTKNPCLHPGDIRVLDAIYEVHFEEKGYLDCIIFPQKGERPHPNECSGGDLDGDQFFVS 849
Query: 842 WDSKLICIRPVKPMSYEPAPTIQLDHDVTIEEVQEYLAKYMVNDGLGGIANAHTVFADKK 901
WD K+I PM Y + +DHDVT+EE+ ++ YM++D LG I+ AH V AD+
Sbjct: 850 WDEKIIPSEMDPPMDYAGSRPRLMDHDVTLEEIHKFFVDYMISDTLGVISTAHLVHADRD 909
Query: 902 PKKAMSAECVELAKLFSIAVDFPKTGVPANLPRSLRVHEYPDFMEKPDKPTYVSNGVLGK 961
P+KA S +C+ELA L S AVDF KTG PA +P +L+ E+PDF+E+ +KPTY+S V GK
Sbjct: 910 PEKARSQKCLELANLHSRAVDFAKTGAPAEMPYALKPREFPDFLERFEKPTYISESVFGK 969
Query: 962 LFRGVKDVSSDINTIEAFTREVATKCYDPDMEVDGFENYLREAFDYKNRYDFKLGNLMDY 1021
L+R VK S + + T YD +E GFE+++ A +++ Y KL +LM Y
Sbjct: 970 LYRAVK---SSLAQRKPEAESEDTVAYDVTLEEAGFESFIETAKAHRDMYGEKLTSLMIY 1029
Query: 1022 YGIKTEPELVSGNI----LRMAKSFDKRNDL-EQISLAMKSLRKEARAWFNEKGSKSTYD 1081
YG E E+++G + + +A+ + D+ ++I+L++K L KEA WF KS D
Sbjct: 1030 YGAANEEEILTGILKTKEMYLARDNRRYGDMKDRITLSVKDLHKEAMGWF----EKSCED 1089
Query: 1082 EDEEYAKASAWYHVTYHPNYWGRYNKGMQRDHFLSFPWCVSDQLIQIKREKMCQISYSQV 1104
E ++ ASAWY+VTY+PN+ ++ FLSFPW V D L+ IK E + S +
Sbjct: 1090 EQQKKKLASAWYYVTYNPNHRD------EKLTFLSFPWIVGDVLLDIKAENAQRQSVEEK 1128
BLAST of Spg008554 vs. ExPASy Swiss-Prot
Match:
Q7XM31 (Probable RNA-dependent RNA polymerase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=RDR2 PE=2 SV=1)
HSP 1 Score: 748.0 bits (1930), Expect = 1.5e-214
Identity = 419/1017 (41.20%), Postives = 608/1017 (59.78%), Query Frame = 0
Query: 108 LGSRISKERMSVIWRGENCCVEFGVGIRKLCFYLSYEFDEYKMELSFENILGVELRCPLD 167
+G R+++ + E G R++ YL ++ YK+E+ FE++ L C LD
Sbjct: 115 VGDRVAERVFEAADAWDGVRAEVIPGKRRVDLYLEHDSQRYKLEVLFEDMKDC-LGCTLD 174
Query: 168 PPSNLLLIQLQGAPRIFRKSSSSS------SAQFYS-KESAGFRWIRDVDFTPSSSIGQS 227
+L+QL APRI S + +F++ KE A F W+R +DFTP+ S G+
Sbjct: 175 -GMGAILLQLNYAPRIHTAISGPAVNSRFMDDRFHACKEDAKFSWVRALDFTPNYSFGRC 234
Query: 228 FTICLEVSPEHQLPSFFETLVGYKERYGPFNLETDSSFSSTSNLVPIISPPQGFDFSYKI 287
T+ L++ + ++L + G + + ++SN+VP++ P+ + Y++
Sbjct: 235 STLVLKLGKSALVSDILKSL-PFSGNLGELTMNSMDGVGASSNVVPLVHCPRDYSVPYEV 294
Query: 288 LFKINALVQHGYLPGPVLDDEFFRLVDPSRFHPDCVEQALEKLFNLKECCYKPKKWLRQQ 347
LF++N+L+ G + ++ + F+ + D + EK+ L+ CY P ++++Q+
Sbjct: 295 LFRLNSLMHMGKIVAKHVNADLFKAL--QELPVDVSRRIFEKMHKLESTCYGPLQFIQQE 354
Query: 348 YLSFYSSKQPSWKPNISLDDG---LVYVHRVQITPSKVYFRGPEANLSNRVVRRFIDDID 407
S S N +G L+ +RV ITPSK++ GPE ++N VV+
Sbjct: 355 AYSMKRSHNVLLS-NEGEGEGERKLMKCYRVNITPSKIFCFGPEEEVTNYVVKHHSAYAS 414
Query: 408 NFLRVSFVDEELDKIRSIDLSPRTSA---ENTERTRVYDRVLSVLRNGIVIGEKKFEFLA 467
+F+RV+FVDE+ K+ S +S R +T +Y R+LS+L+ G IG K FEFLA
Sbjct: 415 DFVRVTFVDEDWSKLSSNAISARIEQGFFSKPFKTGLYYRILSILKKGFSIGPKNFEFLA 474
Query: 468 FSASQLRENSFWMFASREGLTAADIREWMGDFRQIRNVAKYAARLGQSFGSSRKTLCVEE 527
FSASQLR NS WMFAS L A IR WMG F IR+V+K AAR+GQ F SSR+T V
Sbjct: 475 FSASQLRGNSVWMFASNASLNAGGIRRWMGHFENIRSVSKCAARMGQLFSSSRQTFEVLR 534
Query: 528 DEIEVIPDVEVVRKKIKYCFSDGIGKISETLAEKVAEKCGL-ISHTPSAFQIRYAGYKGV 587
++EVIPD+E+ KY FSDGIGKIS A++VA GL ++ PSAFQIRY GYKGV
Sbjct: 535 WDVEVIPDIEITTDGSKYIFSDGIGKISLRFAKRVAHHVGLDPTNLPSAFQIRYGGYKGV 594
Query: 588 VAIDPTSKKKLSLRKSMLKYMSLDTQLDVLSWSKYQPCFLNRQVINLLSTLGIRDHVFVK 647
+AIDP S LSLR SM K+ S L++ SWSK QPC++NR++I+LLSTLGIRD +FV
Sbjct: 595 IAIDPMSSIDLSLRPSMKKFESESRMLNITSWSKSQPCYVNREIISLLSTLGIRDEIFVA 654
Query: 648 KQKEAIDQLDSILHDPSKALEVLELMSPGEMTSILKEMLLFYKPNEEPFLNMMLRTFRAD 707
Q++ + + + +L + AL VL + E + +K +L Y+P+ EP+L+M+L+ + +
Sbjct: 655 MQQDEMRETEEMLTNKEVALSVLGKLGGSETKTAVKMLLQGYEPSSEPYLSMILKAHQEN 714
Query: 708 KLLDLRTKSRIFVPKGRTMMGCLDETQTLEYGQVFVHCSVPGRSSENS------------ 767
+L D+RT+ +I VPKGR ++GCLDET LEYGQV++ + + ++S
Sbjct: 715 RLTDIRTRCKIHVPKGRVLIGCLDETGVLEYGQVYIRITKNSKEQKDSNQSYFYNDDGKT 774
Query: 768 FVVKGKVVVAKNPCLHPGDVRLLDAVDVKALHHMVDCVVFPQKGERPHPNECSGSDLDGD 827
V GKV + KNPCLHPGD+R+L+A+ L MVDC+VFPQ+GERPHPNECSG DLDGD
Sbjct: 775 ATVVGKVAITKNPCLHPGDIRVLEAIYDPDLVGMVDCLVFPQRGERPHPNECSGGDLDGD 834
Query: 828 LYFVCWDSKLICIRPVKPMSYEPAPTIQLDHDVTIEEVQEYLAKYMVNDGLGGIANAHTV 887
LYF+ WD KLI + PM Y +DH VT+EE+Q++ YM+ND LG I+ AH +
Sbjct: 835 LYFITWDDKLIPEKVDTPMDYTATRPRIMDHVVTLEEIQKHFVDYMINDSLGAISTAHLI 894
Query: 888 FADKKPKKAMSAECVELAKLFSIAVDFPKTGVPANLPRSLRVHEYPDFMEKPDKPTYVSN 947
AD+ P KA S EC++LA L S+AVDF KTG PA +PR+LR EYPDFME+ +KP Y+SN
Sbjct: 895 HADRSPLKARSPECLQLATLHSMAVDFAKTGAPAEMPRTLRPREYPDFMERWEKPMYISN 954
Query: 948 GVLGKLFR---GVKDVSSDINTIEAFTREVATKCYDPDMEVDGFENYLREAFDYKNRYDF 1007
GVLGKL+R G + S D + + + + + YDPD+EV G + +L+ A +Y Y+
Sbjct: 955 GVLGKLYRSAMGHMEKSGDSGALSSSSAQ-PSPTYDPDLEVPGSDEFLQAAEEYYELYEE 1014
Query: 1008 KLGNLMDYYGIKTEPELVSGNILRMAKSFDKRND------LEQISLAMKSLRKEARAWFN 1067
KL LM+YY + E E+++GNI R + KR++ ++I A+ +L +EAR W
Sbjct: 1015 KLTTLMNYYRAELEDEILTGNI-RNKMLYLKRDNKRYFEMKDRIVAAVDALHREARGWL- 1074
Query: 1068 EKGSKSTYDEDEEYAKASAWYHVTYHPNYWGRYNKGMQRDHFLSFPWCVSDQLIQIK 1090
S+ E++ ASAWY VTYHP+ + F SFPW D L+ IK
Sbjct: 1075 ----LSSRKEEDASRMASAWYRVTYHPD-------RRRGKRFWSFPWIACDNLLAIK 1111
BLAST of Spg008554 vs. ExPASy Swiss-Prot
Match:
Q9SG02 (RNA-dependent RNA polymerase 6 OS=Arabidopsis thaliana OX=3702 GN=RDR6 PE=1 SV=1)
HSP 1 Score: 588.6 bits (1516), Expect = 1.5e-166
Identity = 394/1059 (37.20%), Postives = 568/1059 (53.64%), Query Frame = 0
Query: 100 RIENVRIHLGSRISKERMSVIWRGENCCVEFGV----GIRKLCFYLSYEFD--------- 159
++ + + +G+ +S++ V WR E V+F V K CF S F
Sbjct: 131 KLAGITLEIGTLVSRDDFFVSWRAEG--VDFLVDPFDNTCKFCFRKSTAFSFKDAVMHAV 190
Query: 160 ---EYKMELSFENILGVELRCPLDPPSNLLLIQLQGAPRIFRKSSSSSSAQFYSKE--SA 219
+YK+EL +I V L +L++QL +PR++ +++ +
Sbjct: 191 INCDYKLELLVRDIQTVRQYKTLH--GFVLILQLASSPRVWYRTADDDIYDTVPGDLLDD 250
Query: 220 GFRWIRDVDFTPSSSIGQSFTICLEVSPEHQ------LPSFFETLVGYKERYGPFNLETD 279
WIR DFT +IG+ + + +SP ++ L F V + P + +
Sbjct: 251 DDPWIRTTDFTQVGAIGRCHSYRVLISPRYENKLRTALDYFRMRRVQEERVRWPPRIRNE 310
Query: 280 SSFSS-TSNLVPIISPPQGFDFSYKILFKINALVQHGYLPGPVLDDEFFRLVDPSRFHPD 339
F S+ I +G S++I+F +N+++ G L + FF L+ R P
Sbjct: 311 PCFGEPVSDHFFCIHHKEG--ISFEIMFLVNSVLHRGVFNQFQLTERFFDLL---RNQPK 370
Query: 340 CVEQA-LEKLFNLKECCYKPKKWLRQQYLSFYSSKQPSWKPNISLDDGLVYVHRVQITPS 399
V A L+ L K + K R + + + K P + + + + R+ ITP+
Sbjct: 371 DVNIASLKHLCTYKRPVFDAYK--RLKLVQEWIQKNPKLLGSHEQSEDISEIRRLVITPT 430
Query: 400 KVYFRGPEANLSNRVVRRFIDDIDNFLRVSFVDEELDKIRSIDLS--------PRTSAEN 459
+ Y PE LSNRV+RR+ + FLRV+F+DE + I S LS TS+
Sbjct: 431 RAYCLPPEVELSNRVLRRYKAVAERFLRVTFMDESMQTINSNVLSYFVAPIVKDLTSSSF 490
Query: 460 TERTRVYDRVLSVLRNGIVIGEKKFEFLAFSASQLRENSFWMFASREGLTAADIREWMGD 519
+++T V+ RV S+L +G + +K+ FLAFSA+QLR+ S W FA +DI+ WMG
Sbjct: 491 SQKTYVFKRVKSILTDGFKLCGRKYSFLAFSANQLRDRSAWFFAEDGKTRVSDIKTWMGK 550
Query: 520 FRQIRNVAKYAARLGQSFGSSRKTLCVEEDEIEV-IPDVEVVRKKIKYCFSDGIGKISET 579
F+ +NVAK AAR+G F S+ T+ V E++ +PD+E + Y FSDGIG I+
Sbjct: 551 FKD-KNVAKCAARMGLCFSSTYATVDVMPHEVDTEVPDIE----RNGYVFSDGIGTITPD 610
Query: 580 LAEKVAEKCGL-ISHTPSAFQIRYAGYKGVVAIDPTSKK--KLSLRKSMLKYMSLDTQLD 639
LA++V EK L + ++P A+QIRYAG+KGVVA P+ +L+LR SM K+ S T L+
Sbjct: 611 LADEVMEKLKLDVHYSPCAYQIRYAGFKGVVARWPSKSDGIRLALRDSMKKFFSKHTILE 670
Query: 640 VLSWSKYQPCFLNRQVINLLSTLGIRDHVFVKKQKEAIDQLDSILHDPSKALEVLELMSP 699
+ SW+++QP FLNRQ+I LLS LG+ D +F Q+ + +L+ IL D A EVL
Sbjct: 671 ICSWTRFQPGFLNRQIITLLSVLGVPDEIFWDMQESMLYKLNRILDDTDVAFEVLTASCA 730
Query: 700 GEMTSILKEMLLFYKPNEEPFLNMMLRTFRADKLLDLRTKSRIFVPKGRTMMGCLDETQT 759
+ + + +KP EP L ML + R +L LR KSRIFV GR +MGCLDE
Sbjct: 731 EQGNTAAIMLSAGFKPKTEPHLRGMLSSVRIAQLWGLREKSRIFVTSGRWLMGCLDEAGI 790
Query: 760 LEYGQVFVHCSVPGRSSENSF---------------VVKGKVVVAKNPCLHPGDVRLLDA 819
LE+GQ F+ S P S EN F VVKG V +AKNPCLHPGDVR+L+A
Sbjct: 791 LEHGQCFIQVSKP--SIENCFSKHGSRFKETKTDLEVVKGYVAIAKNPCLHPGDVRILEA 850
Query: 820 VDVKALHHMVDCVVFPQKGERPHPNECSGSDLDGDLYFVCWDSKLI--CIRPVKPMSYEP 879
VDV LHHM DC++FPQKG+RPH NE SGSDLDGDLYFV WD KLI + M Y+
Sbjct: 851 VDVPQLHHMYDCLIFPQKGDRPHTNEASGSDLDGDLYFVAWDQKLIPPNRKSYPAMHYDA 910
Query: 880 APTIQLDHDVTIEEVQEYLAKYMVNDGLGGIANAHTVFADKKPKKAMSAECVELAKLFSI 939
A L V +++ ++ A+ + N+ LG I NAH V AD+ AM EC+ LA+L +
Sbjct: 911 AEEKSLGRAVNHQDIIDFFARNLANEQLGTICNAHVVHADRSEYGAMDEECLLLAELAAT 970
Query: 940 AVDFPKTGVPANLPRSLRVHEYPDFMEKPDKPTYVSNGVLGKLFRGVKDV-SSDINTIEA 999
AVDFPKTG ++P L+ YPDFM K D TY SN +LG+L+R VK+V D
Sbjct: 971 AVDFPKTGKIVSMPFHLKPKLYPDFMGKEDYQTYKSNKILGRLYRRVKEVYDEDAEASSE 1030
Query: 1000 FTREVATKCYDPDMEVDGFENYLREAFDYKNRYDFKLGNLMDYYGIKTEPELVSGNILRM 1059
+ + + YD +E+ GFE+ + EA+ +K YD +L L+ Y ++ E E+V+G+I M
Sbjct: 1031 ESTDPSAIPYDAVLEIPGFEDLIPEAWGHKCLYDGQLIGLLGQYKVQKEEEIVTGHIWSM 1090
Query: 1060 AKSFDKRND--LEQISLAMKSLRKEARAWFNEK-GSKSTYDEDEE----YAKASAWYHVT 1090
K K+ E++ + SL+KE R F E E+E+ KASAWYHVT
Sbjct: 1091 PKYTSKKQGELKERLKHSYNSLKKEFRKVFEETIPDHENLSEEEKNILYEKKASAWYHVT 1150
BLAST of Spg008554 vs. ExPASy TrEMBL
Match:
A0A6J1JTB1 (RNA-dependent RNA polymerase OS=Cucurbita maxima OX=3661 GN=LOC111487656 PE=3 SV=1)
HSP 1 Score: 1900.6 bits (4922), Expect = 0.0e+00
Identity = 946/1120 (84.46%), Postives = 1007/1120 (89.91%), Query Frame = 0
Query: 3 TIEIFGFPANVAVDDVKEFLENHTGDGTVSTVRISKPKDENSRFTFATVRFTSELAAEYV 62
TIE+FGF V+ +VKEF+ENHTG+GTVS VRISKPKD R TFA V F S++AAEYV
Sbjct: 7 TIEVFGFRREVSAGEVKEFMENHTGEGTVSKVRISKPKDGKGRLTFARVEFRSKVAAEYV 66
Query: 63 VEQAEAEERLWYESSYLKARGVEREMRAAAGRRGEVERIENVRIHLGSRISKERMSVIWR 122
VE+AEAEE LW+ YLKARGVEREMR VER+E VR+ LGS ISKE M VIWR
Sbjct: 67 VEKAEAEEGLWFGRWYLKARGVEREMR--------VERMEKVRVQLGSVISKEEMRVIWR 126
Query: 123 GENCCVEFGVGIRKLCFYLSY--EFDEYKMELSFENILGVELRCPLDPPSNLLLIQLQGA 182
GE VE+G G+RKL FYLSY E D+YKMEL FENILGV+LRCP + SNL LIQLQGA
Sbjct: 127 GEEWSVEYGNGMRKLWFYLSYGVEDDDYKMELRFENILGVQLRCPHNSSSNLFLIQLQGA 186
Query: 183 PRIFRK--SSSSSSAQFYSKESAGFRWIRDVDFTPSSSIGQSFTICLEVSPEHQLPSFFE 242
PRIF+K SSSSSS+QFYSKES GFRWIRDVDFTPSS IGQSF++CLEVSP QLPSFF+
Sbjct: 187 PRIFKKCPSSSSSSSQFYSKESTGFRWIRDVDFTPSSCIGQSFSLCLEVSPGDQLPSFFQ 246
Query: 243 TLVGYKERYGPFNLETDSSFSSTSNLVPIISPPQGFDFSYKILFKINALVQHGYLPGPVL 302
TLVGYKE YGPF L T S+FSSTSNLVPI++PPQ FD YKILFKINALVQHGYLPG L
Sbjct: 247 TLVGYKEIYGPFKLSTGSAFSSTSNLVPIVTPPQPFDIPYKILFKINALVQHGYLPGSAL 306
Query: 303 DDEFFRLVDPSRFHPDCVEQALEKLFNLKECCYKPKKWLRQQYLSFYSSKQPSWKPNISL 362
DD+FFRLVDPSRF D +E ALEKLF+LKECCY+P+KWL+QQYLSF SS Q WK NISL
Sbjct: 307 DDKFFRLVDPSRFRSDYIEYALEKLFSLKECCYEPQKWLKQQYLSFCSSMQLPWKANISL 366
Query: 363 DDGLVYVHRVQITPSKVYFRGPEANLSNRVVRRFIDDIDNFLRVSFVDEELDKIRSIDLS 422
DD LVYVHRVQITP+KVYFRGPEANLSNRVVRRFIDDIDNFLRVSFVDEELDKI S DLS
Sbjct: 367 DDSLVYVHRVQITPAKVYFRGPEANLSNRVVRRFIDDIDNFLRVSFVDEELDKIHSSDLS 426
Query: 423 PRTSAENTERTRVYDRVLSVLRNGIVIGEKKFEFLAFSASQLRENSFWMFASREGLTAAD 482
PRTS E ERTRVYDR+LSVLRNGIVIG KKFEFLAFSASQLRENSFWMFASREGL AAD
Sbjct: 427 PRTSTEKIERTRVYDRILSVLRNGIVIGGKKFEFLAFSASQLRENSFWMFASREGLNAAD 486
Query: 483 IREWMGDFRQIRNVAKYAARLGQSFGSSRKTLCVEEDEIEVIPDVEVVRKKIKYCFSDGI 542
IREWMGDFR+IRNVAKYAARLGQSFGSSRKTLCVEE+EIEVIPD EV RK IKYCFSDGI
Sbjct: 487 IREWMGDFREIRNVAKYAARLGQSFGSSRKTLCVEENEIEVIPDAEVERKNIKYCFSDGI 546
Query: 543 GKISETLAEKVAEKCGLISHTPSAFQIRYAGYKGVVAIDPTSKKKLSLRKSMLKYMSLDT 602
GKISE LAE VAEKCGLISHTPSAFQIRYAGYKGVVA+DPTSKKKLSLRKSMLKYMSLDT
Sbjct: 547 GKISEALAEMVAEKCGLISHTPSAFQIRYAGYKGVVAVDPTSKKKLSLRKSMLKYMSLDT 606
Query: 603 QLDVLSWSKYQPCFLNRQVINLLSTLGIRDHVFVKKQKEAIDQLDSILHDPSKALEVLEL 662
QLDVLSWSKYQPCFLNRQVINLLSTLGIRD VFVK+QK+A+DQLDSIL DPSKALEVLEL
Sbjct: 607 QLDVLSWSKYQPCFLNRQVINLLSTLGIRDDVFVKQQKKAVDQLDSILRDPSKALEVLEL 666
Query: 663 MSPGEMTSILKEMLLFYKPNEEPFLNMMLRTFRADKLLDLRTKSRIFVPKGRTMMGCLDE 722
MSPGEMTS+LK+MLLFY P+EEPFLNMMLRTFRADKLLDLRTKSRIFVPKGRTMMGCLDE
Sbjct: 667 MSPGEMTSVLKDMLLFYNPHEEPFLNMMLRTFRADKLLDLRTKSRIFVPKGRTMMGCLDE 726
Query: 723 TQTLEYGQVFVHCSVPGRSSENSFVVKGKVVVAKNPCLHPGDVRLLDAVDVKALHHMVDC 782
T+TLEYGQVF+HCSVPGRSSE++FVVKGKVVVAKNPCLHPGDVR+LDAVDVK LHHMVDC
Sbjct: 727 TRTLEYGQVFIHCSVPGRSSESNFVVKGKVVVAKNPCLHPGDVRVLDAVDVKTLHHMVDC 786
Query: 783 VVFPQKGERPHPNECSGSDLDGDLYFVCWDSKLICIRPVKPMSYEPAPTIQLDHDVTIEE 842
VVFPQKG+RPHPNECSGSDLDGDLYFVCWD +L CIRP+KPMSYEPAPTIQLDHDVT+EE
Sbjct: 787 VVFPQKGKRPHPNECSGSDLDGDLYFVCWDPELTCIRPIKPMSYEPAPTIQLDHDVTLEE 846
Query: 843 VQEYLAKYMVNDGLGGIANAHTVFADKKPKKAMSAECVELAKLFSIAVDFPKTGVPANLP 902
VQEY A YMVNDGLGGIANAHTVFAD KPKKAMSAEC+ELAKLFSIAVDFPKTGVPANLP
Sbjct: 847 VQEYFANYMVNDGLGGIANAHTVFADMKPKKAMSAECIELAKLFSIAVDFPKTGVPANLP 906
Query: 903 RSLRVHEYPDFMEKPDKPTYVSNGVLGKLFRGVKDVSSDINTIEAFTREVATKCYDPDME 962
RSLRVHEYPDFMEKP+K TY+SNGV+GKLFRGVKDVSSDIN IE FTREVA KCYDPDME
Sbjct: 907 RSLRVHEYPDFMEKPNKRTYISNGVIGKLFRGVKDVSSDINNIETFTREVAVKCYDPDME 966
Query: 963 VDGFENYLREAFDYKNRYDFKLGNLMDYYGIKTEPELVSGNILRMAKSFDKRNDLEQISL 1022
V GFENYL EAFDYK YDFKLGNLMDYYGI+TEPELVSGNILRM KSFDKRND+EQISL
Sbjct: 967 VMGFENYLSEAFDYKRMYDFKLGNLMDYYGIETEPELVSGNILRMGKSFDKRNDMEQISL 1026
Query: 1023 AMKSLRKEARAWFNEKGSKSTYD----EDEEYAKASAWYHVTYHPNYWGRYNKGMQRDHF 1082
AM+SLRKEAR WFNEK SKS Y+ ED EYAKASAWYHVTYHPN+WGRYN+GMQRDHF
Sbjct: 1027 AMRSLRKEARGWFNEKASKSIYNNNKGEDNEYAKASAWYHVTYHPNFWGRYNEGMQRDHF 1086
Query: 1083 LSFPWCVSDQLIQIKREKMCQISYSQVSSLIHKFGDLSFY 1115
LSFPWCVSD+LIQIKREKM S VSSLIHKFG LSFY
Sbjct: 1087 LSFPWCVSDKLIQIKREKM---ESSPVSSLIHKFGKLSFY 1115
BLAST of Spg008554 vs. ExPASy TrEMBL
Match:
A0A6J1EB47 (RNA-dependent RNA polymerase OS=Cucurbita moschata OX=3662 GN=LOC111430971 PE=3 SV=1)
HSP 1 Score: 1888.6 bits (4891), Expect = 0.0e+00
Identity = 942/1121 (84.03%), Postives = 1008/1121 (89.92%), Query Frame = 0
Query: 3 TIEIFGFPANVAVDDVKEFLENHTGDGTVSTVRISKPKDENSRFTFATVRFTSELAAEYV 62
TIE+FGF V+ +VKEF+ENHTG+GTVS VRISKPK E SR T A V F S++AAEYV
Sbjct: 7 TIEVFGFGREVSAGEVKEFMENHTGEGTVSKVRISKPKYEKSRLTSARVEFRSKVAAEYV 66
Query: 63 VEQAEAEERLWYESSYLKARGVEREMRAAAGRRGEVERIENVRIHLGSRISKERMSVIWR 122
VE+AEAEERLW+ YLKAR VERE R R +ER+E VR+ LGS I K+ M VIWR
Sbjct: 67 VEKAEAEERLWFGRWYLKARRVERE-REREMRVERLERLEKVRVQLGSVIWKDEMRVIWR 126
Query: 123 GENCCVEFGVGIRKLCFYLSY--EFDEYKMELSFENILGVELRCPLDPPSNLLLIQLQGA 182
GE+ VE+G G+RKL FYLSY E D+YKMEL FENILGV+LRCP + SNL LIQLQGA
Sbjct: 127 GEDWSVEYGNGMRKLWFYLSYGVEDDDYKMELRFENILGVQLRCPHNSSSNLFLIQLQGA 186
Query: 183 PRIFRK---SSSSSSAQFYSKESAGFRWIRDVDFTPSSSIGQSFTICLEVSPEHQLPSFF 242
PRIF+K SSSSSS+QFYSKES GFRWIRDVDFTPSS IGQSF +C EVSP QLPSFF
Sbjct: 187 PRIFKKCPSSSSSSSSQFYSKESTGFRWIRDVDFTPSSCIGQSFALCFEVSPGDQLPSFF 246
Query: 243 ETLVGYKERYGPFNLETDSSFSSTSNLVPIISPPQGFDFSYKILFKINALVQHGYLPGPV 302
+TLVGYKE YGPF L T S+FSSTSNLVPI++PPQ F YKILFKINALVQHGYLPG
Sbjct: 247 QTLVGYKEIYGPFKLSTGSAFSSTSNLVPIVTPPQPFHIPYKILFKINALVQHGYLPGSA 306
Query: 303 LDDEFFRLVDPSRFHPDCVEQALEKLFNLKECCYKPKKWLRQQYLSFYSSKQPSWKPNIS 362
LDD+FFRLVDP+RF D +E ALEKLF+LKECCY+P+KWL+QQYLSF SS Q WK NIS
Sbjct: 307 LDDKFFRLVDPTRFRSDYIEYALEKLFSLKECCYEPQKWLKQQYLSFCSSMQLPWKANIS 366
Query: 363 LDDGLVYVHRVQITPSKVYFRGPEANLSNRVVRRFIDDIDNFLRVSFVDEELDKIRSIDL 422
LDD LVYVHRVQITP+KVYFRGPEANLSNRVVRRFIDDIDNFLRVSFVDEELDKI S DL
Sbjct: 367 LDDSLVYVHRVQITPAKVYFRGPEANLSNRVVRRFIDDIDNFLRVSFVDEELDKIYSSDL 426
Query: 423 SPRTSAENTERTRVYDRVLSVLRNGIVIGEKKFEFLAFSASQLRENSFWMFASREGLTAA 482
SPRTS E ERTRVYDR+LSVLRNGIVIG KKFEFLAFSASQLRENSFWMFASREGL AA
Sbjct: 427 SPRTSTEKIERTRVYDRILSVLRNGIVIGGKKFEFLAFSASQLRENSFWMFASREGLNAA 486
Query: 483 DIREWMGDFRQIRNVAKYAARLGQSFGSSRKTLCVEEDEIEVIPDVEVVRKKIKYCFSDG 542
DIREWMGDFR+IRNVAKYAARLGQSFGSSRKTLCVEE+EIEVIPDVEV RK IKYCFSDG
Sbjct: 487 DIREWMGDFREIRNVAKYAARLGQSFGSSRKTLCVEENEIEVIPDVEVERKNIKYCFSDG 546
Query: 543 IGKISETLAEKVAEKCGLISHTPSAFQIRYAGYKGVVAIDPTSKKKLSLRKSMLKYMSLD 602
IGKISE LAE VAEKCGLISHTPSAFQIRYAGYKGVVA+DPTSKKKLSLRKSMLKYMSLD
Sbjct: 547 IGKISEALAEMVAEKCGLISHTPSAFQIRYAGYKGVVAVDPTSKKKLSLRKSMLKYMSLD 606
Query: 603 TQLDVLSWSKYQPCFLNRQVINLLSTLGIRDHVFVKKQKEAIDQLDSILHDPSKALEVLE 662
TQLDVLSWSKYQPCFLNRQVINLLSTLGI+D VFVK+QK+A+DQLDSIL DPSKALEVLE
Sbjct: 607 TQLDVLSWSKYQPCFLNRQVINLLSTLGIQDDVFVKQQKKAVDQLDSILRDPSKALEVLE 666
Query: 663 LMSPGEMTSILKEMLLFYKPNEEPFLNMMLRTFRADKLLDLRTKSRIFVPKGRTMMGCLD 722
LMSPGEMTS+LK+MLLFY P+EEPFLNMMLRTFRADKLLDLRTKSRIFVP+GRTMMGCLD
Sbjct: 667 LMSPGEMTSVLKDMLLFYNPHEEPFLNMMLRTFRADKLLDLRTKSRIFVPEGRTMMGCLD 726
Query: 723 ETQTLEYGQVFVHCSVPGRSSENSFVVKGKVVVAKNPCLHPGDVRLLDAVDVKALHHMVD 782
ET+TLEYGQVF+HCSVPGRSSE++FVVKGKVVVAKNPCLHPGDVR+LDAVDVK LHHMVD
Sbjct: 727 ETRTLEYGQVFIHCSVPGRSSESNFVVKGKVVVAKNPCLHPGDVRVLDAVDVKTLHHMVD 786
Query: 783 CVVFPQKGERPHPNECSGSDLDGDLYFVCWDSKLICIRPVKPMSYEPAPTIQLDHDVTIE 842
CVVFPQKG+RPHPNECSGSDLDGDLYFVCWD +L CIRP+KPMSYEPAPTIQLDHDVTIE
Sbjct: 787 CVVFPQKGKRPHPNECSGSDLDGDLYFVCWDPELTCIRPIKPMSYEPAPTIQLDHDVTIE 846
Query: 843 EVQEYLAKYMVNDGLGGIANAHTVFADKKPKKAMSAECVELAKLFSIAVDFPKTGVPANL 902
EVQEY A YMVNDGLGGIANAHTVFADKKPKKAMSAEC+ELAKLFSIAVDFPKTGVPANL
Sbjct: 847 EVQEYFANYMVNDGLGGIANAHTVFADKKPKKAMSAECIELAKLFSIAVDFPKTGVPANL 906
Query: 903 PRSLRVHEYPDFMEKPDKPTYVSNGVLGKLFRGVKDVSSDINTIEAFTREVATKCYDPDM 962
PRSLRVHEYPDFMEKP+K TY SNGV+GKLFRGVKDVSSDIN IE FTREVA KCYDPDM
Sbjct: 907 PRSLRVHEYPDFMEKPNKRTYTSNGVIGKLFRGVKDVSSDINNIETFTREVAAKCYDPDM 966
Query: 963 EVDGFENYLREAFDYKNRYDFKLGNLMDYYGIKTEPELVSGNILRMAKSFDKRNDLEQIS 1022
EV GFENYL EAFDYK+ YDFKLGNLMDYYGI+TEPELVSGNILRM KSFDKRND+EQIS
Sbjct: 967 EVMGFENYLSEAFDYKSMYDFKLGNLMDYYGIETEPELVSGNILRMGKSFDKRNDMEQIS 1026
Query: 1023 LAMKSLRKEARAWFNEKGSKSTY----DEDEEYAKASAWYHVTYHPNYWGRYNKGMQRDH 1082
LAM+SLRKEAR WFNEKGSKS Y DED+EYAKASAWYHVTYHPN+WGRYN+GMQRDH
Sbjct: 1027 LAMRSLRKEARGWFNEKGSKSIYNNNKDEDDEYAKASAWYHVTYHPNFWGRYNEGMQRDH 1086
Query: 1083 FLSFPWCVSDQLIQIKREKMCQISYSQVSSLIHKFGDLSFY 1115
FLSFPWCVS++LIQIKREKM S VSSLIHKFG LSFY
Sbjct: 1087 FLSFPWCVSEKLIQIKREKM---ESSPVSSLIHKFGKLSFY 1123
BLAST of Spg008554 vs. ExPASy TrEMBL
Match:
A0A6J1FF44 (RNA-dependent RNA polymerase OS=Cucurbita moschata OX=3662 GN=LOC111444909 PE=3 SV=1)
HSP 1 Score: 1877.1 bits (4861), Expect = 0.0e+00
Identity = 924/1103 (83.77%), Postives = 1000/1103 (90.66%), Query Frame = 0
Query: 3 TIEIFGFPANVAVDDVKEFLENHTGDGTVSTVRISKPKDENSRFTFATVRFTSELAAEYV 62
TIEI+GFP V D+VKEF+ENHTGDGTV TVRISK DE +RFTFATV+FTS+L EYV
Sbjct: 10 TIEIYGFPTQVTADEVKEFIENHTGDGTVLTVRISKLNDEKARFTFATVQFTSKLGGEYV 69
Query: 63 VEQAEAEERLWYESSYLKARGVEREMRAAAGRRGEVERIENVRIHLGSRISKERMSVIWR 122
V QA AE+RLW+ SSYLKAR VERE++ AA GE+ER+ENV + GS +SKE M VIW+
Sbjct: 70 VAQAAAEKRLWFGSSYLKARKVEREIKTAARVHGELERMENVNVQWGSLVSKEEMKVIWK 129
Query: 123 GENCCVEFGVGIRKLCFYLSYEFDEYKMELSFENILGVELRCPLDPPSNLLLIQLQGAPR 182
G VE+G+G+RKL FYLSYE EYKMEL FENILGV+LRC +D S L LIQLQGAPR
Sbjct: 130 GGKWSVEYGIGVRKLGFYLSYEGVEYKMELCFENILGVQLRCSVDRGSKLFLIQLQGAPR 189
Query: 183 IFRKSSSSSSAQFYSKESAGFRWIRDVDFTPSSSIGQSFTICLEVSPEHQLPSFFETLVG 242
IF+K+ SSS++ S+ES GFRWIRDVDFTPSS IGQSF +CLE+S QLPSFF+TLVG
Sbjct: 190 IFKKAPLSSSSRLSSEESTGFRWIRDVDFTPSSCIGQSFALCLELSHGDQLPSFFQTLVG 249
Query: 243 YKERYGPFNLETDSSFSSTSNLVPIISPPQGFDFSYKILFKINALVQHGYLPGPVLDDEF 302
YKE Y PF L+T SS SS SNLVPII+PPQGFD YKILFKINAL+QHGYLPGP LD+EF
Sbjct: 250 YKESYAPFILQTGSSLSSISNLVPIITPPQGFDIPYKILFKINALLQHGYLPGPALDNEF 309
Query: 303 FRLVDPSRFHPDCVEQALEKLFNLKECCYKPKKWLRQQYLSFYSSKQPSWKPNISLDDGL 362
FRLVD SRF PD VE AL+KLF+ KECCY+P+KWL+QQYLSFYSSKQ WKPNISLDDGL
Sbjct: 310 FRLVDSSRFRPDYVEYALDKLFHFKECCYEPQKWLKQQYLSFYSSKQLPWKPNISLDDGL 369
Query: 363 VYVHRVQITPSKVYFRGPEANLSNRVVRRFIDDIDNFLRVSFVDEELDKIRSIDLSPRTS 422
VYVHRVQITP+KVYFRGPEANLSNRVVR FIDD+DNFLRVSFVDEELDK+ SIDLSPRTS
Sbjct: 370 VYVHRVQITPTKVYFRGPEANLSNRVVRHFIDDVDNFLRVSFVDEELDKLHSIDLSPRTS 429
Query: 423 AENTERTRVYDRVLSVLRNGIVIGEKKFEFLAFSASQLRENSFWMFASREGLTAADIREW 482
AEN RTRVYDRVLSVLRNGIVIG+KKFEFLAFSASQLRENS WMFASR+GL+AADIREW
Sbjct: 430 AENDLRTRVYDRVLSVLRNGIVIGDKKFEFLAFSASQLRENSCWMFASRKGLSAADIREW 489
Query: 483 MGDFRQIRNVAKYAARLGQSFGSSRKTLCVEEDEIEVIPDVEVVRKKIKYCFSDGIGKIS 542
MGD RQIRNVAKYAARLGQSFGSSR+TLCVE+ EIEVI DVEV KI YCFSDGIGKIS
Sbjct: 490 MGDLRQIRNVAKYAARLGQSFGSSRRTLCVEQHEIEVISDVEVETNKITYCFSDGIGKIS 549
Query: 543 ETLAEKVAEKCGLISHTPSAFQIRYAGYKGVVAIDPTSKKKLSLRKSMLKYMSLDTQLDV 602
TLAEKVAEKCGLISHTPSAFQIRYAGYKGVVAIDPTS KKLSLRKSMLKY SLDTQ+DV
Sbjct: 550 GTLAEKVAEKCGLISHTPSAFQIRYAGYKGVVAIDPTSTKKLSLRKSMLKYTSLDTQVDV 609
Query: 603 LSWSKYQPCFLNRQVINLLSTLGIRDHVFVKKQKEAIDQLDSILHDPSKALEVLELMSPG 662
LSWSKYQPCFLNRQVINLLSTLGI+D VFVKKQKEAIDQLDSIL DPS+ALEVLELMSPG
Sbjct: 610 LSWSKYQPCFLNRQVINLLSTLGIKDRVFVKKQKEAIDQLDSILEDPSRALEVLELMSPG 669
Query: 663 EMTSILKEMLLFYKPNEEPFLNMMLRTFRADKLLDLRTKSRIFVPKGRTMMGCLDETQTL 722
EMTSILKE+LL YKPNEEPFLNMMLRTFRA KLLDLRTKSRIFVPKGRTMMGCLDET+TL
Sbjct: 670 EMTSILKELLLLYKPNEEPFLNMMLRTFRAAKLLDLRTKSRIFVPKGRTMMGCLDETRTL 729
Query: 723 EYGQVFVHCSVPGRSSENSFVVKGKVVVAKNPCLHPGDVRLLDAVDVKALHHMVDCVVFP 782
EYGQVFVHCS+PGRSSE++FVVKGKVVVAKNPCLHPGDVRLLDAVDVKALHHMVDCVVFP
Sbjct: 730 EYGQVFVHCSLPGRSSESNFVVKGKVVVAKNPCLHPGDVRLLDAVDVKALHHMVDCVVFP 789
Query: 783 QKGERPHPNECSGSDLDGDLYFVCWDSKLICIRPVKPMSYEPAPTIQLDHDVTIEEVQEY 842
QKG+RPHPNECSGSDLDGDLYFV WD +L I+PVKPMSYEPAPT+QLDHDVTIEEVQ Y
Sbjct: 790 QKGKRPHPNECSGSDLDGDLYFVGWDPELTRIKPVKPMSYEPAPTMQLDHDVTIEEVQGY 849
Query: 843 LAKYMVNDGLGGIANAHTVFADKKPKKAMSAECVELAKLFSIAVDFPKTGVPANLPRSLR 902
L KYMVNDGLGGIANAHTVFADKKPKKAM+AEC++LAKLFSIAVDFPKTGVPAN P +LR
Sbjct: 850 LVKYMVNDGLGGIANAHTVFADKKPKKAMAAECIKLAKLFSIAVDFPKTGVPANFPHNLR 909
Query: 903 VHEYPDFMEKPDKPTYVSNGVLGKLFRGVKDVSSDINTIEAFTREVATKCYDPDMEVDGF 962
V EYPDFMEKPDKPTYVSNGVLGKLFRGVKDVSSD+NT+E F+REVATKCYDPDMEVDGF
Sbjct: 910 VREYPDFMEKPDKPTYVSNGVLGKLFRGVKDVSSDVNTVEIFSREVATKCYDPDMEVDGF 969
Query: 963 ENYLREAFDYKNRYDFKLGNLMDYYGIKTEPELVSGNILRMAKSFDKRNDLEQISLAMKS 1022
E+YL EAF++K RYDFKLGNLMDYYGIKTEPEL+SGNILRMAKSFDKRND+EQISLAMKS
Sbjct: 970 EDYLSEAFEFKARYDFKLGNLMDYYGIKTEPELISGNILRMAKSFDKRNDMEQISLAMKS 1029
Query: 1023 LRKEARAWFNEKGSKSTYDED----EEYAKASAWYHVTYHPNYWGRYNKGMQRDHFLSFP 1082
LRKEAR+WFNEKGSKS YDED EEYAKASAWY VTYHP+YWGRYN+GMQRDHFLSFP
Sbjct: 1030 LRKEARSWFNEKGSKSAYDEDDNKNEEYAKASAWYRVTYHPDYWGRYNEGMQRDHFLSFP 1089
Query: 1083 WCVSDQLIQIKREKMCQISYSQV 1102
WCVSD+LIQIKREK C +++S V
Sbjct: 1090 WCVSDKLIQIKREKTCLVNFSPV 1112
BLAST of Spg008554 vs. ExPASy TrEMBL
Match:
A0A6J1K1Q3 (RNA-dependent RNA polymerase OS=Cucurbita maxima OX=3661 GN=LOC111489826 PE=3 SV=1)
HSP 1 Score: 1871.7 bits (4847), Expect = 0.0e+00
Identity = 928/1106 (83.91%), Postives = 1000/1106 (90.42%), Query Frame = 0
Query: 4 IEIFGFPANVAVDDVKEFLENHTGDGTVSTVRISKPKDENSRFTFATVRFTSELAAEYVV 63
IEI+GFPA V D+VKEF+ENHTGDGTV TVRISKP DE +RFTFATVRFTS+L EYVV
Sbjct: 11 IEIYGFPAEVTADEVKEFIENHTGDGTVLTVRISKPNDEKARFTFATVRFTSKLGGEYVV 70
Query: 64 EQAEAEERLWYESSYLKARGVEREMR-----AAAGRRGEVERIENVRIHLGSRISKERMS 123
QA AE+RLW+ SSYLKAR VERE+R AAA G +ER+ENV + GS +SKE+M
Sbjct: 71 AQAAAEKRLWFGSSYLKARKVEREIRTATAAAAARVHGGLERMENVNVQWGSLVSKEKMK 130
Query: 124 VIWRGENCCVEFGVGIRKLCFYLSYEFDEYKMELSFENILGVELRCPLDPPSNLLLIQLQ 183
VIW+ VE+G+G RKL FYLSYE EYKMEL FENILGV+LRC D S L LIQLQ
Sbjct: 131 VIWKEGKWSVEYGIGARKLGFYLSYEGVEYKMELCFENILGVQLRCSGDRGSELFLIQLQ 190
Query: 184 GAPRIFRKSSSSSSAQFYSKESAGFRWIRDVDFTPSSSIGQSFTICLEVSPEHQLPSFFE 243
GAPRIF+K+ SSSS++ S+ES GFRWIRDVDFTPSS IGQSF +CLE+SP QLPSFF
Sbjct: 191 GAPRIFKKTPSSSSSRLSSEESTGFRWIRDVDFTPSSCIGQSFALCLELSPGDQLPSFFR 250
Query: 244 TLVGYKERYGPFNLETDSSFSSTSNLVPIISPPQGFDFSYKILFKINALVQHGYLPGPVL 303
TLVGYKE Y PF L SS SS SNLVPII+PPQGFD SYKILFKINAL+QHGYLPGP L
Sbjct: 251 TLVGYKESYAPFILHPGSSLSSISNLVPIITPPQGFDISYKILFKINALLQHGYLPGPAL 310
Query: 304 DDEFFRLVDPSRFHPDCVEQALEKLFNLKECCYKPKKWLRQQYLSFYSSKQPSWKPNISL 363
D+EFFRLVD SRF P+ +E ALEKLF+ KECCY+P+KWL+QQYLSFYSSKQ KPN+SL
Sbjct: 311 DNEFFRLVDSSRFRPEYIEYALEKLFHFKECCYEPQKWLKQQYLSFYSSKQLPCKPNVSL 370
Query: 364 DDGLVYVHRVQITPSKVYFRGPEANLSNRVVRRFIDDIDNFLRVSFVDEELDKIRSIDLS 423
DDGLVYVHRVQITP+KVYFRGPEANLSNRVVR FIDD+DNFLRVSFVDEELDK+ SIDLS
Sbjct: 371 DDGLVYVHRVQITPTKVYFRGPEANLSNRVVRHFIDDVDNFLRVSFVDEELDKLHSIDLS 430
Query: 424 PRTSAENTERTRVYDRVLSVLRNGIVIGEKKFEFLAFSASQLRENSFWMFASREGLTAAD 483
PRTSAEN RTRVYDRVLSVLRNGIVIG+KKFEFLAFSASQLRENS WMFASR+GL+AAD
Sbjct: 431 PRTSAENDLRTRVYDRVLSVLRNGIVIGDKKFEFLAFSASQLRENSCWMFASRKGLSAAD 490
Query: 484 IREWMGDFRQIRNVAKYAARLGQSFGSSRKTLCVEEDEIEVIPDVEVVRKKIKYCFSDGI 543
IREWMGDFRQIRNVAKYAARLGQSFGSSR+TLCVE+ EIEVIPDVEV KI YCFSDGI
Sbjct: 491 IREWMGDFRQIRNVAKYAARLGQSFGSSRRTLCVEQHEIEVIPDVEVETNKITYCFSDGI 550
Query: 544 GKISETLAEKVAEKCGLISHTPSAFQIRYAGYKGVVAIDPTSKKKLSLRKSMLKYMSLDT 603
GKIS TLAEKVAEKCGLISHTPSAFQIRYAGYKGVVAIDPTS KKLSLRKSMLKY SLDT
Sbjct: 551 GKISGTLAEKVAEKCGLISHTPSAFQIRYAGYKGVVAIDPTSTKKLSLRKSMLKYTSLDT 610
Query: 604 QLDVLSWSKYQPCFLNRQVINLLSTLGIRDHVFVKKQKEAIDQLDSILHDPSKALEVLEL 663
Q+DVLSWSKYQPCFLNRQVINLLSTLGI+D VFVKKQKEAIDQLDSIL DPS+ALEVLEL
Sbjct: 611 QVDVLSWSKYQPCFLNRQVINLLSTLGIKDRVFVKKQKEAIDQLDSILEDPSRALEVLEL 670
Query: 664 MSPGEMTSILKEMLLFYKPNEEPFLNMMLRTFRADKLLDLRTKSRIFVPKGRTMMGCLDE 723
MSPGEMTSILKE+LL YKPNEEPFLNMMLRTFRA KLLDLRTKSRIFVPKGRTMMGCLDE
Sbjct: 671 MSPGEMTSILKELLLLYKPNEEPFLNMMLRTFRAAKLLDLRTKSRIFVPKGRTMMGCLDE 730
Query: 724 TQTLEYGQVFVHCSVPGRSSENSFVVKGKVVVAKNPCLHPGDVRLLDAVDVKALHHMVDC 783
T+TLEYGQVFVHCS+PGRSSE++FVVKGKVVVAKNPCLHPGDVRLLDAVDVKALHHMV+C
Sbjct: 731 TRTLEYGQVFVHCSLPGRSSESNFVVKGKVVVAKNPCLHPGDVRLLDAVDVKALHHMVNC 790
Query: 784 VVFPQKGERPHPNECSGSDLDGDLYFVCWDSKLICIRPVKPMSYEPAPTIQLDHDVTIEE 843
VVFPQKGERPHPNECSGSDLDGDLYFV WD +L I+PVKPMSYEPAPT++LDHDVTIEE
Sbjct: 791 VVFPQKGERPHPNECSGSDLDGDLYFVGWDPELTRIKPVKPMSYEPAPTMRLDHDVTIEE 850
Query: 844 VQEYLAKYMVNDGLGGIANAHTVFADKKPKKAMSAECVELAKLFSIAVDFPKTGVPANLP 903
VQEYLAKYMVNDGLGGIANAHTVFADKKPKKAM+AEC++LAKLFSIAVDFPKTGVPAN P
Sbjct: 851 VQEYLAKYMVNDGLGGIANAHTVFADKKPKKAMTAECIKLAKLFSIAVDFPKTGVPANFP 910
Query: 904 RSLRVHEYPDFMEKPDKPTYVSNGVLGKLFRGVKDVSSDINTIEAFTREVATKCYDPDME 963
+LRV EYPDFMEKPDKPTYVSNGVLGKLFRGVKDVSSD+NTIE F+REVATKCYDPDME
Sbjct: 911 HNLRVREYPDFMEKPDKPTYVSNGVLGKLFRGVKDVSSDVNTIEIFSREVATKCYDPDME 970
Query: 964 VDGFENYLREAFDYKNRYDFKLGNLMDYYGIKTEPELVSGNILRMAKSFDKRNDLEQISL 1023
VDGFE+YL EAF+YK RYDFKLGNLMDYYGIKTEPEL+SGNILRMAKSFDKRNDLEQISL
Sbjct: 971 VDGFEDYLSEAFEYKARYDFKLGNLMDYYGIKTEPELISGNILRMAKSFDKRNDLEQISL 1030
Query: 1024 AMKSLRKEARAWFNEKGSKSTYDE---DEEYAKASAWYHVTYHPNYWGRYNKGMQRDHFL 1083
A+KSLRKEAR+WFNEKGSKS DE DEEYAKASAWY VTYHP+YWGRYN+GMQRDHFL
Sbjct: 1031 AIKSLRKEARSWFNEKGSKSACDEDDKDEEYAKASAWYRVTYHPDYWGRYNEGMQRDHFL 1090
Query: 1084 SFPWCVSDQLIQIKREKMCQISYSQV 1102
SFPWCVSD+LIQIKREK C ++ S +
Sbjct: 1091 SFPWCVSDKLIQIKREKTCLVNLSPI 1116
BLAST of Spg008554 vs. ExPASy TrEMBL
Match:
A0A0S1YCZ4 (RNA-dependent RNA polymerase OS=Cucumis sativus OX=3659 GN=RDR1c PE=2 SV=1)
HSP 1 Score: 1833.5 bits (4748), Expect = 0.0e+00
Identity = 909/1109 (81.97%), Postives = 984/1109 (88.73%), Query Frame = 0
Query: 3 TIEIFGFPANVAVDDVKEFLENHTGDGTVSTVRISKPKDENSRFTFATVRFTSELAAEYV 62
TIEI+GF V D+VKEFLENHTGDGTVSTVRISKPKDE +RFT TV F S+LAAEY+
Sbjct: 4 TIEIYGFRPQVTADEVKEFLENHTGDGTVSTVRISKPKDEKARFTSVTVLFKSKLAAEYI 63
Query: 63 VEQAEAEER-LWYESSYLKARGVEREMRAAAGRRG--EVERIENVRIHLGSRISKERMSV 122
V ++ EER LW+ESSYLKAR +E+ + A +G E+ER+E+V+ HLGS IS +M V
Sbjct: 64 VAKSTTEERKLWFESSYLKARELEKAV-VANETKGVLEMERMEDVKGHLGSMISNGKMRV 123
Query: 123 IWRGENCCVEFGVGIRKLCFYLSYEFDEYKMELSFENILGVELRCPLDPPSNLLLIQLQG 182
IW GE VEFG G RKL FYLSYE DEYKMEL FENIL VE RCPL+ PS LIQLQG
Sbjct: 124 IWEGEKWSVEFGNGFRKLWFYLSYEVDEYKMELCFENILSVEFRCPLNEPSKFFLIQLQG 183
Query: 183 APRIFRKSSSSSSAQFYSKESAGFRWIRDVDFTPSSSIGQSFTICLEVSPEHQLPSFFET 242
APRIFRK+ SSSS+ YS +S FRWIRDVDFTPSS IGQSFTICL++SP H LP FF+T
Sbjct: 184 APRIFRKTPSSSSSPLYSNKSTSFRWIRDVDFTPSSCIGQSFTICLQLSPSHHLPPFFQT 243
Query: 243 LVGYKERYGPFNLETDSSFSSTSNLVPIISPPQGFDFSYKILFKINALVQHGYLPGPVLD 302
LVGYK Y PF L SS S SNLVPII+PPQ FD SYKILFKINAL+QHGYL GP LD
Sbjct: 244 LVGYKVTYAPFILHKGSSLVSNSNLVPIITPPQAFDISYKILFKINALLQHGYLSGPTLD 303
Query: 303 DEFFRLVDPSRFHPDCVEQALEKLFNLKECCYKPKKWLRQQYLSFYSSKQPSWKPNISLD 362
DEFFRLVD SRFH D +E ALEKLFNLKECCYKP+KWL+ QYLS+Y+S Q WK NISLD
Sbjct: 304 DEFFRLVDSSRFHSDYIEHALEKLFNLKECCYKPQKWLKDQYLSYYTSNQLPWKSNISLD 363
Query: 363 DGLVYVHRVQITPSKVYFRGPEANLSNRVVRRFIDDIDNFLRVSFVDEELDKIRSIDLSP 422
DGLVYVHRVQITP KVYF GPEANLSNRVVRRFI DIDNFLRVSFVDEELDK+ SIDL+P
Sbjct: 364 DGLVYVHRVQITPLKVYFCGPEANLSNRVVRRFIGDIDNFLRVSFVDEELDKLHSIDLAP 423
Query: 423 RTSA-ENTERTRVYDRVLSVLRNGIVIGEKKFEFLAFSASQLRENSFWMFASREGLTAAD 482
R+S+ EN++RTRVYDRV+S+L+NGIVIG KKFEFLAFSASQLRENSFWMFASREGL+AAD
Sbjct: 424 RSSSPENSQRTRVYDRVISILKNGIVIGNKKFEFLAFSASQLRENSFWMFASREGLSAAD 483
Query: 483 IREWMGDFRQIRNVAKYAARLGQSFGSSRKTLCVEEDEIEVIPDVEVVRKKIKYCFSDGI 542
IREWMGDF IRNVAKYAARLGQSFGSSRKTLCVEE EIEVIPDVEV RK I YCFSDGI
Sbjct: 484 IREWMGDFHHIRNVAKYAARLGQSFGSSRKTLCVEEHEIEVIPDVEVERKNIMYCFSDGI 543
Query: 543 GKISETLAEKVAEKCGLISHTPSAFQIRYAGYKGVVAIDPTSKKKLSLRKSMLKYMSLDT 602
GKIS+TLA+KVAEKCGL SHTPSAFQIRYAGYKGVVAIDPTS+KKLSLRKSMLKYMSLDT
Sbjct: 544 GKISKTLAKKVAEKCGLTSHTPSAFQIRYAGYKGVVAIDPTSEKKLSLRKSMLKYMSLDT 603
Query: 603 QLDVLSWSKYQPCFLNRQVINLLSTLGIRDHVFVKKQKEAIDQLDSILHDPSKALEVLEL 662
QLDVL WSKYQPCFLNRQVINLLSTLGIRD VFVKKQKEAIDQLDSIL DPS+ALEVLEL
Sbjct: 604 QLDVLLWSKYQPCFLNRQVINLLSTLGIRDDVFVKKQKEAIDQLDSILEDPSRALEVLEL 663
Query: 663 MSPGEMTSILKEMLLFYKPNEEPFLNMMLRTFRADKLLDLRTKSRIFVPKGRTMMGCLDE 722
MSPGEMTSILKE+L FY PN+EPFLNMMLRTFRADKLLDLRTKSRIFVPKGRTMMGCLDE
Sbjct: 664 MSPGEMTSILKELLSFYMPNQEPFLNMMLRTFRADKLLDLRTKSRIFVPKGRTMMGCLDE 723
Query: 723 TQTLEYGQVFVHCSVPGRSSENSFVVKGKVVVAKNPCLHPGDVRLLDAVDVKALHHMVDC 782
TQTLEYGQVFVHCS+PGRSSE +FVVKGKVVVAKNPCLHPGDVRLLDA+DVKALHHMVDC
Sbjct: 724 TQTLEYGQVFVHCSIPGRSSEGNFVVKGKVVVAKNPCLHPGDVRLLDAIDVKALHHMVDC 783
Query: 783 VVFPQKGERPHPNECSGSDLDGDLYFVCWDSKLICIRPVKPMSYEPAPTIQLDHDVTIEE 842
VVFPQKG+RPHPNECSGSDLDGDLYFVCWD++L CI+ VKPMSY+PAPTIQLDHDVTIEE
Sbjct: 784 VVFPQKGKRPHPNECSGSDLDGDLYFVCWDTELTCIKQVKPMSYKPAPTIQLDHDVTIEE 843
Query: 843 VQEYLAKYMVNDGLGGIANAHTVFADKKPKKAMSAECVELAKLFSIAVDFPKTGVPANLP 902
VQEY A YMVNDGLG IANAHTVFADK KKAMSAEC++LAKLFSIAVDFPKTGVPANLP
Sbjct: 844 VQEYFANYMVNDGLGAIANAHTVFADKNSKKAMSAECIKLAKLFSIAVDFPKTGVPANLP 903
Query: 903 RSLRVHEYPDFMEKPDKPTYVSNGVLGKLFRGVKDVSSDINTIEAFTREVATKCYDPDME 962
R+LRVHEYPDFM+KP+K TYVSNGVLGKLFRGVKDVSSD++ E FTREVATKCYDPDME
Sbjct: 904 RNLRVHEYPDFMDKPNKATYVSNGVLGKLFRGVKDVSSDVSAFEIFTREVATKCYDPDME 963
Query: 963 VDGFENYLREAFDYKNRYDFKLGNLMDYYGIKTEPELVSGNILRMAKSFDKRNDLEQISL 1022
VDGFE YLREAFDYK +YDFKLGNLMDYYGIKTEPELVSGNIL+MAKSFDKR DLEQI+
Sbjct: 964 VDGFEKYLREAFDYKTKYDFKLGNLMDYYGIKTEPELVSGNILKMAKSFDKRKDLEQIAF 1023
Query: 1023 AMKSLRKEARAWFNEKGSKSTYDE-DEEYAKASAWYHVTYHPNYWGRYNKGMQRDHFLSF 1082
AMKSLRKE R WFNE SKSTYD+ +EYA+ASAWY VTYHP+YWG YN+G +RDHFLSF
Sbjct: 1024 AMKSLRKEVRFWFNENESKSTYDDIQDEYARASAWYCVTYHPDYWGCYNEGTKRDHFLSF 1083
Query: 1083 PWCVSDQLIQIKREKMCQISYSQVSSLIH 1107
PWCV+D+LIQIKREKM + S SSL+H
Sbjct: 1084 PWCVADKLIQIKREKMSMRNSSPKSSLLH 1111
BLAST of Spg008554 vs. TAIR 10
Match:
AT1G14790.1 (RNA-dependent RNA polymerase 1 )
HSP 1 Score: 1255.4 bits (3247), Expect = 0.0e+00
Identity = 635/1098 (57.83%), Postives = 805/1098 (73.32%), Query Frame = 0
Query: 3 TIEIFGFPANVAVDDVKEFLENHTGDGTVSTVRISKPKDENSRFTFATVRFTSELAAEYV 62
TI++FGFP V+ ++VK+FLE TG GTV +++ +PK R +A V+FTSE +
Sbjct: 4 TIQVFGFPNGVSAEEVKKFLERLTGSGTVYAIKVRQPKKGGPR-VYAIVQFTSERHTRLI 63
Query: 63 VEQAEAEERLWYESSYLKARGVEREMRAAAGRRGEVERIENVRIHLGSRISKERMSVIWR 122
+ A ERL+Y SYLKA VE+++ R + I +++ G ++S ++ +W
Sbjct: 64 I--TAAAERLYYGRSYLKAFEVEQDI--VPKPRASLHTISGLKMFFGCQVSTKKFLTLWS 123
Query: 123 GENCCVEFGVGIRKLCFYLSYEFDEYKMELSFENILGVELRCPLDPPSNLLLIQLQGAPR 182
++ CV FG+G+RKL F S+ +Y++ELS+ENI ++L P S L+IQ+ GAP+
Sbjct: 124 AQDVCVSFGIGMRKLHFSFSWYQKDYRLELSYENIWQIDLHSPQGRSSKFLVIQVIGAPK 183
Query: 183 IFRKSSSSSSAQF----YSKESAGFRWIRDVDFTPSSSIGQSFTICLEVSPEHQLPSFFE 242
IF K + F + + + +WIR DFT SS IGQS CLE+ +P F E
Sbjct: 184 IFEKEDQPINLLFGIMDFYSDGSDEQWIRTTDFTSSSCIGQSTAFCLELPVHLNVPDFRE 243
Query: 243 TLVGYKE-RYGPFNLETDSSFSSTSN-LVPIISPPQGFDFSYKILFKINALVQHGYLPGP 302
Y E R F +E+ SS+SS +N LVP++ PP GF ++ILFK+N LVQ+ L GP
Sbjct: 244 NFANYAEHRASSFLIESGSSYSSNANTLVPVVDPPPGFSLPFEILFKLNTLVQNACLSGP 303
Query: 303 VLDDEFFRLVDPSRFHPDCVEQALEKLFNLKECCYKPKKWLRQQYLSFYSSKQPSWKPNI 362
LD +F+RL++ ++ ++ LEKLF+L ECCY+P WLR +Y + S + P I
Sbjct: 304 ALDLDFYRLLNQKKYDRALIDHCLEKLFHLGECCYEPAHWLRDEYKKWISKGKLPLSPTI 363
Query: 363 SLDDGLVYVHRVQITPSKVYFRGPEANLSNRVVRRFIDDIDNFLRVSFVDEELDKIRSID 422
SLDDGLVY++RVQ+TP++VYF GPE N+SNRV+R + I+NFLRVSFVDE+L+K+RS+D
Sbjct: 364 SLDDGLVYMYRVQVTPARVYFSGPEVNVSNRVLRHYSKYINNFLRVSFVDEDLEKVRSMD 423
Query: 423 LSPRTSAENTERTRVYDRVLSVLRNGIVIGEKKFEFLAFSASQLRENSFWMFASREGLTA 482
LSPR+S + RT++YDR+ SVLR+GIVIG+KKFEFLAFS+SQLRENS WMFA + +TA
Sbjct: 424 LSPRSSTQ--RRTKLYDRIYSVLRDGIVIGDKKFEFLAFSSSQLRENSAWMFAPIDRITA 483
Query: 483 ADIREWMGDFRQIRNVAKYAARLGQSFGSSRKTLCVEEDEIEVIPDVEVVRKKIKYCFSD 542
A IR WMGDF IRNVAKYAARLGQSF SSR+TL V DEIEVIPDVE++ +Y FSD
Sbjct: 484 AHIRAWMGDFDHIRNVAKYAARLGQSFSSSRETLNVRSDEIEVIPDVEIISLGTRYVFSD 543
Query: 543 GIGKISETLAEKVAEKCGLISHTPSAFQIRYAGYKGVVAIDPTSKKKLSLRKSMLKYMSL 602
GIGKIS A KVA KCGL +PSAFQIRY GYKGVVA+DP S KKLSLRKSM K+ S
Sbjct: 544 GIGKISAEFARKVARKCGLTEFSPSAFQIRYGGYKGVVAVDPNSSKKLSLRKSMSKFESE 603
Query: 603 DTQLDVLSWSKYQPCFLNRQVINLLSTLGIRDHVFVKKQKEAIDQLDSILHDPSKALEVL 662
+T+LDVL+WSKYQPC++NRQ+I LLSTLG+ D VF KKQ+E +D+LD+IL P +A E L
Sbjct: 604 NTKLDVLAWSKYQPCYMNRQLITLLSTLGVTDSVFEKKQREVVDRLDAILTHPLEAHEAL 663
Query: 663 ELMSPGEMTSILKEMLLF-YKPNEEPFLNMMLRTFRADKLLDLRTKSRIFVPKGRTMMGC 722
LM+PGE T+ILK ++L YKP+ EPFL+MML+ FRA KLL+LRTK+RIF+ GR+MMGC
Sbjct: 664 GLMAPGENTNILKALILCGYKPDAEPFLSMMLQNFRASKLLELRTKTRIFISGGRSMMGC 723
Query: 723 LDETQTLEYGQVFVHCSVPGRSSENSFVVKGKVVVAKNPCLHPGDVRLLDAVDVKALHHM 782
LDET+TLEYGQV V S P R F++ G VVVAKNPCLHPGDVR+L AV+V AL+HM
Sbjct: 724 LDETRTLEYGQVVVQYSDPMRPGRR-FIITGPVVVAKNPCLHPGDVRVLQAVNVPALNHM 783
Query: 783 VDCVVFPQKGERPHPNECSGSDLDGDLYFVCWDSKLICIRPVKPMSYEPAPTIQLDHDVT 842
VDCVVFPQKG RPHPNECSGSDLDGD+YFVCWD +L+ R +PM Y P PT LDHDVT
Sbjct: 784 VDCVVFPQKGLRPHPNECSGSDLDGDIYFVCWDQELVPPRTSEPMDYTPEPTQILDHDVT 843
Query: 843 IEEVQEYLAKYMVNDGLGGIANAHTVFADKKPKKAMSAECVELAKLFSIAVDFPKTGVPA 902
IEEV+EY A Y+VND LG IANAHT FADK+P KA S C+ELAK FS AVDFPKTGV A
Sbjct: 844 IEEVEEYFANYIVNDSLGIIANAHTAFADKEPLKAFSDPCIELAKKFSTAVDFPKTGVAA 903
Query: 903 NLPRSLRVHEYPDFMEKPDKPTYVSNGVLGKLFRGVKDVSSDINTIEAFTREVATKCYDP 962
+P+ L V EYPDFMEKPDKPTY S V+GKLFR VK+ + + +I++FT +VA+K YD
Sbjct: 904 VIPQHLYVKEYPDFMEKPDKPTYESKNVIGKLFREVKERAPPLISIKSFTLDVASKSYDK 963
Query: 963 DMEVDGFENYLREAFDYKNRYDFKLGNLMDYYGIKTEPELVSGNILRMAKSFDKRNDLEQ 1022
DMEVDGFE Y+ EAF K YDFKLGNLMDYYGIKTE E++SG I+RM+KSF KR D E
Sbjct: 964 DMEVDGFEEYVDEAFYQKANYDFKLGNLMDYYGIKTEAEILSGGIMRMSKSFTKRRDAES 1023
Query: 1023 ISLAMKSLRKEARAWFNEKGSKSTYDEDEEYAKASAWYHVTYHPNYWGRYNKGMQRDHFL 1082
I A+++LRKE + FN ++ +E+ E AKASAWYHVTYH +YWG YN+G+ RDHFL
Sbjct: 1024 IGRAVRALRKETLSLFN-----ASEEEENESAKASAWYHVTYHSSYWGLYNEGLNRDHFL 1083
Query: 1083 SFPWCVSDQLIQIKREKM 1094
SF WCV D+L++IK+ +
Sbjct: 1084 SFAWCVYDKLVRIKKTNL 1088
BLAST of Spg008554 vs. TAIR 10
Match:
AT4G11130.1 (RNA-dependent RNA polymerase 2 )
HSP 1 Score: 765.0 bits (1974), Expect = 8.4e-221
Identity = 451/1142 (39.49%), Postives = 663/1142 (58.06%), Query Frame = 0
Query: 2 ATIEIFGFPANVAVDDVKEFLENHTGDGTVSTVRISKPKDENSRFTFATVRFTSELAAEY 61
+T++I P + D++ FLE H G+ TV + I +D FA V+FT+ L +
Sbjct: 10 STVKISNVPQTIVADELLRFLELHLGEDTVFALEIPTTRDNWKPRDFARVQFTT-LEVKS 69
Query: 62 VVEQAEAEERLWYESSYLKARGVEREM--RAAAGRRGEVERIENVRIHLGSRISKERMSV 121
+ ++ +L +++ L+ ++ R R+ R++++ + +G S E+
Sbjct: 70 RAQLLSSQSKLLFKTHNLRLSEAYDDIIPRPVDPRK----RLDDIVLTVGFPESDEKRFC 129
Query: 122 I---WRGENCCVEFGVGIRKLCFYLSYEFDEYKMELSFENILGVELRCPLDPPSNL--LL 181
W G C + R++ F++ D YK+E+ FE+I+ C S + L
Sbjct: 130 ALEKWDGVRCWIL--TEKRRVEFWVWESGDCYKIEVRFEDIIETLSCCVNGDASEIDAFL 189
Query: 182 IQLQGAPRIFRKSSSSSSAQF------YSKESAGFRWIRDVDFTPSSSIGQSFTICLEVS 241
++L+ P++F++ + + +F + KE F WIR DF+ S SIG S CLEV
Sbjct: 190 LKLKYGPKVFKRVTVHIATKFKSDRYRFCKEDFDFMWIRTTDFSGSKSIGTSTCFCLEVH 249
Query: 242 PEHQLPSFFETLVGYKERYGPFNLETDSSFSSTSNLVPIISPP-QGFDFSYKILFKINAL 301
+ F L Y+E +F+S + +VP+++ G +F Y+ILF++NAL
Sbjct: 250 NGSTMLDIFSGLPYYREDTLSLTYVDGKTFASAAQIVPLLNAAILGLEFPYEILFQLNAL 309
Query: 302 VQHGYLP-GPVLDDEFFRLVDPSRFHPDCVEQALEKLFNLKECCYKPKKWLRQQYLSFYS 361
V + D E +++ V L+KL CY P +++ Q S
Sbjct: 310 VHAQKISLFAASDMELIKILRGMSLETALV--ILKKLHQQSSICYDPVFFVKTQMQSVVK 369
Query: 362 SKQ--PSWKPNISLDDGLVYVHRVQITPSKVYFRGPEANLSNRVVRRFIDDIDNFLRVSF 421
+ P+ + ++ R +TPSK+Y GPE +N VV+ F + + +F+RV+F
Sbjct: 370 KMKHSPASAYKRLTEQNIMSCQRAYVTPSKIYLLGPELETANYVVKNFAEHVSDFMRVTF 429
Query: 422 VDEELDKIRSIDLSPRTSA---ENTERTRVYDRVLSVLRNGIVIGEKKFEFLAFSASQLR 481
V+E+ K+ + LS + RT +Y+RVLS+L GI +G K+FEFLAFSASQLR
Sbjct: 430 VEEDWSKLPANALSVNSKEGYFVKPSRTNIYNRVLSILGEGITVGPKRFEFLAFSASQLR 489
Query: 482 ENSFWMFASREGLTAADIREWMGDFRQIRNVAKYAARLGQSFGSSRKTLCVEEDEIEVIP 541
NS WMFAS E + A DIREWMG FR+IR+++K AAR+GQ F +SR+TL V ++E IP
Sbjct: 490 GNSVWMFASNEKVKAEDIREWMGCFRKIRSISKCAARMGQLFSASRQTLIVRAQDVEQIP 549
Query: 542 DVEVVRKKIKYCFSDGIGKISETLAEKVAEKCGLISHTPSAFQIRYAGYKGVVAIDPTSK 601
D+EV YCFSDGIGKIS A++VA+KCGL SH PSAFQIRY GYKGV+A+D +S
Sbjct: 550 DIEVTTDGADYCFSDGIGKISLAFAKQVAQKCGL-SHVPSAFQIRYGGYKGVIAVDRSSF 609
Query: 602 KKLSLRKSMLKYMSLDTQLDVLSWSKYQPCFLNRQVINLLSTLGIRDHVFVKKQKEAIDQ 661
+KLSLR SMLK+ S + L+V W++ PCFLNR++I LLSTLGI D +F Q +
Sbjct: 610 RKLSLRDSMLKFDSNNRMLNVTRWTESMPCFLNREIICLLSTLGIEDAMFEAMQAVHLSM 669
Query: 662 LDSILHDPSKALEVLELMSPGEMTSILKEMLL-FYKPNEEPFLNMMLRTFRADKLLDLRT 721
L ++L D AL VL+ +S ++L +MLL Y P+ EP+L+MMLR +L +L++
Sbjct: 670 LGNMLEDRDAALNVLQKLSGENSKNLLVKMLLQGYAPSSEPYLSMMLRVHHESQLSELKS 729
Query: 722 KSRIFVPKGRTMMGCLDETQTLEYGQVFVHCSVP------------GRSSENSFVVKGKV 781
+ RI VPKGR ++GC+DE LEYGQV+V ++ + E + VV GKV
Sbjct: 730 RCRILVPKGRILIGCMDEMGILEYGQVYVRVTLTKAELKSRDQSYFRKIDEETSVVIGKV 789
Query: 782 VVAKNPCLHPGDVRLLDAVDVKALHH--MVDCVVFPQKGERPHPNECSGSDLDGDLYFVC 841
VV KNPCLHPGD+R+LDA+ +DC++FPQKGERPHPNECSG DLDGD +FV
Sbjct: 790 VVTKNPCLHPGDIRVLDAIYEVHFEEKGYLDCIIFPQKGERPHPNECSGGDLDGDQFFVS 849
Query: 842 WDSKLICIRPVKPMSYEPAPTIQLDHDVTIEEVQEYLAKYMVNDGLGGIANAHTVFADKK 901
WD K+I PM Y + +DHDVT+EE+ ++ YM++D LG I+ AH V AD+
Sbjct: 850 WDEKIIPSEMDPPMDYAGSRPRLMDHDVTLEEIHKFFVDYMISDTLGVISTAHLVHADRD 909
Query: 902 PKKAMSAECVELAKLFSIAVDFPKTGVPANLPRSLRVHEYPDFMEKPDKPTYVSNGVLGK 961
P+KA S +C+ELA L S AVDF KTG PA +P +L+ E+PDF+E+ +KPTY+S V GK
Sbjct: 910 PEKARSQKCLELANLHSRAVDFAKTGAPAEMPYALKPREFPDFLERFEKPTYISESVFGK 969
Query: 962 LFRGVKDVSSDINTIEAFTREVATKCYDPDMEVDGFENYLREAFDYKNRYDFKLGNLMDY 1021
L+R VK S + + T YD +E GFE+++ A +++ Y KL +LM Y
Sbjct: 970 LYRAVK---SSLAQRKPEAESEDTVAYDVTLEEAGFESFIETAKAHRDMYGEKLTSLMIY 1029
Query: 1022 YGIKTEPELVSGNI----LRMAKSFDKRNDL-EQISLAMKSLRKEARAWFNEKGSKSTYD 1081
YG E E+++G + + +A+ + D+ ++I+L++K L KEA WF KS D
Sbjct: 1030 YGAANEEEILTGILKTKEMYLARDNRRYGDMKDRITLSVKDLHKEAMGWF----EKSCED 1089
Query: 1082 EDEEYAKASAWYHVTYHPNYWGRYNKGMQRDHFLSFPWCVSDQLIQIKREKMCQISYSQV 1104
E ++ ASAWY+VTY+PN+ ++ FLSFPW V D L+ IK E + S +
Sbjct: 1090 EQQKKKLASAWYYVTYNPNHRD------EKLTFLSFPWIVGDVLLDIKAENAQRQSVEEK 1128
BLAST of Spg008554 vs. TAIR 10
Match:
AT3G49500.1 (RNA-dependent RNA polymerase 6 )
HSP 1 Score: 588.6 bits (1516), Expect = 1.1e-167
Identity = 394/1059 (37.20%), Postives = 568/1059 (53.64%), Query Frame = 0
Query: 100 RIENVRIHLGSRISKERMSVIWRGENCCVEFGV----GIRKLCFYLSYEFD--------- 159
++ + + +G+ +S++ V WR E V+F V K CF S F
Sbjct: 131 KLAGITLEIGTLVSRDDFFVSWRAEG--VDFLVDPFDNTCKFCFRKSTAFSFKDAVMHAV 190
Query: 160 ---EYKMELSFENILGVELRCPLDPPSNLLLIQLQGAPRIFRKSSSSSSAQFYSKE--SA 219
+YK+EL +I V L +L++QL +PR++ +++ +
Sbjct: 191 INCDYKLELLVRDIQTVRQYKTLH--GFVLILQLASSPRVWYRTADDDIYDTVPGDLLDD 250
Query: 220 GFRWIRDVDFTPSSSIGQSFTICLEVSPEHQ------LPSFFETLVGYKERYGPFNLETD 279
WIR DFT +IG+ + + +SP ++ L F V + P + +
Sbjct: 251 DDPWIRTTDFTQVGAIGRCHSYRVLISPRYENKLRTALDYFRMRRVQEERVRWPPRIRNE 310
Query: 280 SSFSS-TSNLVPIISPPQGFDFSYKILFKINALVQHGYLPGPVLDDEFFRLVDPSRFHPD 339
F S+ I +G S++I+F +N+++ G L + FF L+ R P
Sbjct: 311 PCFGEPVSDHFFCIHHKEG--ISFEIMFLVNSVLHRGVFNQFQLTERFFDLL---RNQPK 370
Query: 340 CVEQA-LEKLFNLKECCYKPKKWLRQQYLSFYSSKQPSWKPNISLDDGLVYVHRVQITPS 399
V A L+ L K + K R + + + K P + + + + R+ ITP+
Sbjct: 371 DVNIASLKHLCTYKRPVFDAYK--RLKLVQEWIQKNPKLLGSHEQSEDISEIRRLVITPT 430
Query: 400 KVYFRGPEANLSNRVVRRFIDDIDNFLRVSFVDEELDKIRSIDLS--------PRTSAEN 459
+ Y PE LSNRV+RR+ + FLRV+F+DE + I S LS TS+
Sbjct: 431 RAYCLPPEVELSNRVLRRYKAVAERFLRVTFMDESMQTINSNVLSYFVAPIVKDLTSSSF 490
Query: 460 TERTRVYDRVLSVLRNGIVIGEKKFEFLAFSASQLRENSFWMFASREGLTAADIREWMGD 519
+++T V+ RV S+L +G + +K+ FLAFSA+QLR+ S W FA +DI+ WMG
Sbjct: 491 SQKTYVFKRVKSILTDGFKLCGRKYSFLAFSANQLRDRSAWFFAEDGKTRVSDIKTWMGK 550
Query: 520 FRQIRNVAKYAARLGQSFGSSRKTLCVEEDEIEV-IPDVEVVRKKIKYCFSDGIGKISET 579
F+ +NVAK AAR+G F S+ T+ V E++ +PD+E + Y FSDGIG I+
Sbjct: 551 FKD-KNVAKCAARMGLCFSSTYATVDVMPHEVDTEVPDIE----RNGYVFSDGIGTITPD 610
Query: 580 LAEKVAEKCGL-ISHTPSAFQIRYAGYKGVVAIDPTSKK--KLSLRKSMLKYMSLDTQLD 639
LA++V EK L + ++P A+QIRYAG+KGVVA P+ +L+LR SM K+ S T L+
Sbjct: 611 LADEVMEKLKLDVHYSPCAYQIRYAGFKGVVARWPSKSDGIRLALRDSMKKFFSKHTILE 670
Query: 640 VLSWSKYQPCFLNRQVINLLSTLGIRDHVFVKKQKEAIDQLDSILHDPSKALEVLELMSP 699
+ SW+++QP FLNRQ+I LLS LG+ D +F Q+ + +L+ IL D A EVL
Sbjct: 671 ICSWTRFQPGFLNRQIITLLSVLGVPDEIFWDMQESMLYKLNRILDDTDVAFEVLTASCA 730
Query: 700 GEMTSILKEMLLFYKPNEEPFLNMMLRTFRADKLLDLRTKSRIFVPKGRTMMGCLDETQT 759
+ + + +KP EP L ML + R +L LR KSRIFV GR +MGCLDE
Sbjct: 731 EQGNTAAIMLSAGFKPKTEPHLRGMLSSVRIAQLWGLREKSRIFVTSGRWLMGCLDEAGI 790
Query: 760 LEYGQVFVHCSVPGRSSENSF---------------VVKGKVVVAKNPCLHPGDVRLLDA 819
LE+GQ F+ S P S EN F VVKG V +AKNPCLHPGDVR+L+A
Sbjct: 791 LEHGQCFIQVSKP--SIENCFSKHGSRFKETKTDLEVVKGYVAIAKNPCLHPGDVRILEA 850
Query: 820 VDVKALHHMVDCVVFPQKGERPHPNECSGSDLDGDLYFVCWDSKLI--CIRPVKPMSYEP 879
VDV LHHM DC++FPQKG+RPH NE SGSDLDGDLYFV WD KLI + M Y+
Sbjct: 851 VDVPQLHHMYDCLIFPQKGDRPHTNEASGSDLDGDLYFVAWDQKLIPPNRKSYPAMHYDA 910
Query: 880 APTIQLDHDVTIEEVQEYLAKYMVNDGLGGIANAHTVFADKKPKKAMSAECVELAKLFSI 939
A L V +++ ++ A+ + N+ LG I NAH V AD+ AM EC+ LA+L +
Sbjct: 911 AEEKSLGRAVNHQDIIDFFARNLANEQLGTICNAHVVHADRSEYGAMDEECLLLAELAAT 970
Query: 940 AVDFPKTGVPANLPRSLRVHEYPDFMEKPDKPTYVSNGVLGKLFRGVKDV-SSDINTIEA 999
AVDFPKTG ++P L+ YPDFM K D TY SN +LG+L+R VK+V D
Sbjct: 971 AVDFPKTGKIVSMPFHLKPKLYPDFMGKEDYQTYKSNKILGRLYRRVKEVYDEDAEASSE 1030
Query: 1000 FTREVATKCYDPDMEVDGFENYLREAFDYKNRYDFKLGNLMDYYGIKTEPELVSGNILRM 1059
+ + + YD +E+ GFE+ + EA+ +K YD +L L+ Y ++ E E+V+G+I M
Sbjct: 1031 ESTDPSAIPYDAVLEIPGFEDLIPEAWGHKCLYDGQLIGLLGQYKVQKEEEIVTGHIWSM 1090
Query: 1060 AKSFDKRND--LEQISLAMKSLRKEARAWFNEK-GSKSTYDEDEE----YAKASAWYHVT 1090
K K+ E++ + SL+KE R F E E+E+ KASAWYHVT
Sbjct: 1091 PKYTSKKQGELKERLKHSYNSLKKEFRKVFEETIPDHENLSEEEKNILYEKKASAWYHVT 1150
BLAST of Spg008554 vs. TAIR 10
Match:
AT2G19930.1 (RNA-dependent RNA polymerase family protein )
HSP 1 Score: 138.3 bits (347), Expect = 3.8e-32
Identity = 180/710 (25.35%), Postives = 296/710 (41.69%), Query Frame = 0
Query: 359 DDGLVYVHRVQITPSKVY-------FRGPEANLSNRVVRRFIDDIDNFLRVSFVDEELDK 418
D G + ++ + P+ Y +GP + + + + D DN L V F D +
Sbjct: 198 DSGKTHYYQCNVAPNGSYTFKVLSALQGPLLEHTGTHLHKVLGD-DNVLTVKFADVQ--- 257
Query: 419 IRSIDLSPRTSAENTERTRVYDRVLSVLRNGIVIGEKKFEFLAFSASQLRE--------- 478
+ +T Y + +NGI+IG ++++F F E
Sbjct: 258 ----------KSSSTYSIDHYFTYKGIAKNGIMIGLRRYQFFVFKDGGKEEKKKDLSTKK 317
Query: 479 ----------NSFWMFASREGLTAADIREWMGDFRQIR---NVAKYAARLGQSFGSSRKT 538
+F+ + LT I E F + +A Y AR S KT
Sbjct: 318 VKCYFIRTDSTAFYDMQNPYILTGKSIYEARMHFMHVHRAPTLANYMARFSLIL-SKTKT 377
Query: 539 LCVEEDEI--EVIPDV--------EVVRKKIKYCF-SDGIGKISETLA------------ 598
L V+ I + I D+ +V+ K K C SDG G ISE LA
Sbjct: 378 LEVDMTGITFDQIDDIHCHDQDGKDVLDKNKKPCIHSDGTGYISEDLARMCPLNIFKGKC 437
Query: 599 ---EKVAEKCGLISHTPSAFQIRYAGY--KGVVAID-PTSKKKLSLRKSMLKY------- 658
E + E C F++ Y GY KG ++ + + +R SM+K
Sbjct: 438 LRSESIQEACYQDPPLLIQFRMFYDGYAVKGTFLLNKKLCPRTVQVRPSMIKVSKDPSLS 497
Query: 659 -MSLDTQLDVLSWSK-YQPCFLNRQVINLLSTLGIRDHVFVKKQKEAIDQLDSILHDPSK 718
S L+V++ S + L++ ++ LLS GI + F+ +++ SI ++
Sbjct: 498 NFSTFNALEVVTTSNPPKRTKLSKNLVALLSYGGIPNEFFLDILLNTLEESKSIFYNKRA 557
Query: 719 ALEVLELMSPGEM-TSILKEMLLFYKPNEEPFLNMMLRTFRADKLLDLRTKSRIFVPKGR 778
AL ++ GEM +M+L P +EP L L + DL+ ++ V +
Sbjct: 558 ALNA--ALNYGEMDDQNAAQMILVGIPLDEPHLKNYLSILLKTEKNDLKA-GKLPVTESY 617
Query: 779 TMMGCLDETQTLEYGQVFVHCSVPGRSSENSFVVKGKVVVAKNPCLHPGDVRLLDAVDVK 838
+MG +D T L+ +V V S + G+V+V +NP LH GD+ +L A VK
Sbjct: 618 YLMGTVDPTGALKEDEVCVIL--------ESGQISGEVLVYRNPGLHFGDIHILKATYVK 677
Query: 839 ALHHMVD----CVVFPQKGERPHPNECSGSDLDGDLYFVCWDSKLI-CIRPVKP-MSYEP 898
AL V V FPQKG R +E +G D DGD+YF+ + +L+ +P +P +S P
Sbjct: 678 ALEEYVGNSKFAVFFPQKGPRSLGDEIAGGDFDGDMYFISRNPELLENFKPSEPWVSLTP 737
Query: 899 --------APTIQLDHDVTIEEVQEYL--AKYMVNDGLGGIANAHTVFADK--------- 958
AP+ QL + EE+ E A + ++ +G A++ D+
Sbjct: 738 PSKSNSGRAPS-QLSPEELEEELFEMFLTAGFHASNVIGIAADSWLTIMDRFLILGDDRA 797
Query: 959 KPKKAMSAECVELAKLFSIAVDFPKTGVPANLPRSLRVHEYPDFMEKPDKPTYVSNGVLG 969
+ K M + +EL ++ A+D PK G LP L+ +P +ME+ K + S +LG
Sbjct: 798 EEKAEMKKKMLELIDIYYDALDAPKKGDKVYLPNKLKPDIFPHYMERDKK--FQSTSILG 857
BLAST of Spg008554 vs. TAIR 10
Match:
AT2G19910.1 (RNA-dependent RNA polymerase family protein )
HSP 1 Score: 135.2 bits (339), Expect = 3.2e-31
Identity = 188/813 (23.12%), Postives = 328/813 (40.34%), Query Frame = 0
Query: 320 LEKLFNLKECCYKPKKW--LRQQYLSFYSSKQPSWKPNISLDDGLVYVHRVQITPSKVY- 379
+ K +L Y+ W L Q+Y S ++P + D G+ + ++ + Y
Sbjct: 173 IRKWKDLPMVSYEAAVWNRLGQRYCSPKERRRP-----LEGDSGMTHYYQCHVATDGSYK 232
Query: 380 FRGPEANLSNRVVRRFIDDIDNFLRVSFVDEELDKIRSIDLSPRTSAENTERTRVYDRVL 439
F+G + + + + D DN L V F DK+ ++ T +Y
Sbjct: 233 FKGHLLENTGTHLHKVLGD-DNVLTVKF-----DKVLGVE---------TYCNDLYSTYK 292
Query: 440 SVLRNGIVIGEKKFEFLAF-------------------------SASQLRENSFWMFASR 499
+ +NGI++G +++ F F S + + + ++FA
Sbjct: 293 GIAKNGIMVGLRRYRFFVFKDGGKEEKKKDVSTKGVKCYFIRTDSTASIDMQNPYIFA-- 352
Query: 500 EGLTAADIREWMGDFRQIRNVAKYAARLGQSFGSSRKTLCVEEDEI--EVIPDV------ 559
G + + R + ++ Y +R S KTL V+ I E I D+
Sbjct: 353 -GKSMHEARMHFMHVNTLSSLPNYMSRFSLIL-SKTKTLEVDMTGITFEQIDDIHCHDQD 412
Query: 560 --EVVRKKIKYCF-SDGIGKISETLA---------------EKVAEK---------CGLI 619
+V+ K K C SDG G ISE LA + K CG
Sbjct: 413 DKDVLDKNGKPCIHSDGTGYISEDLARMCPVNIFKGKSMRSNNIQSKNLNFEGQGPCGQE 472
Query: 620 SHTPSAFQIRYAGYKGVVAIDPTSKK----KLSLRKSMLKY--------MSLDTQLDVLS 679
F+I Y GY V T+KK + +R SM+K +S L+V++
Sbjct: 473 PPLLIQFRIFYNGY-AVKGTFLTNKKLPPRTVQVRPSMIKVYEDRTLSNLSTFNSLEVVT 532
Query: 680 WSK-YQPCFLNRQVINLLSTLGIRDHVFVKKQKEAIDQLDSILHDPSKALEVLELMSPGE 739
S + L+R ++ LLS G+ + F+ + +++ +I + A + +
Sbjct: 533 TSNPPRKARLSRNLVALLSYGGVPNDFFLNILRNTLEESKTIFYSERAAFKAAINYGDDQ 592
Query: 740 MTSILKEMLLFYKPNEEPFLNMMLRTFRADKLLDLRTKSRIFVPKGRTMMGCLDETQTLE 799
T+ +M+L P +EP+L L + L+ R + + +MG +D T L+
Sbjct: 593 YTA---DMILVGIPLDEPYLKDRLSYLLKTERNALKA-GRFPIDESYYIMGTVDPTGELK 652
Query: 800 YGQVFVHCSVPGRSSENSFVVKGKVVVAKNPCLHPGDVRLLDAVDVKALHHMVD----CV 859
++ V +S + G V+V +NP LH GD+ +L A VKAL V V
Sbjct: 653 ENEICVIL--------HSGQISGDVLVYRNPGLHFGDIHVLKATYVKALEDYVGNAKFAV 712
Query: 860 VFPQKGERPHPNECSGSDLDGDLYFVCWDSKLI-CIRPVKPMSYEPAPTI----QLDHDV 919
FPQKG R +E +G D DGD+YF+ + KL+ +P +P P+ + ++
Sbjct: 713 FFPQKGPRSLGDEIAGGDFDGDMYFISRNPKLLEHFKPSEPWVSSSKPSKIYCGRKPSEL 772
Query: 920 TIEEVQEYL------AKYMVNDGLG-------GIANAHTVFADK--KPKKAMSAECVELA 979
+ EE++E L A++ D +G GI + D+ K K ++L
Sbjct: 773 SEEELEEELFKMFLKARFCKRDVIGMAADCWLGIMDPFLTLGDESAKEKYERKKNILKLI 832
Query: 980 KLFSIAVDFPKTGVPANLPRSLRVHEYPDFMEKPDKPTYVSNGVLGKLFRGVKDVSSDIN 1033
++ A+D PK G +LP L + +P +ME+ K + S +LG +F D N
Sbjct: 833 DIYYDALDAPKKGAKVDLPPDLEIKNFPHYMERDPKRDFRSTSILGLIF----DTVDSHN 892
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| XP_038875555.1 | 0.0e+00 | 84.53 | probable RNA-dependent RNA polymerase 1 [Benincasa hispida] | [more] |
| XP_022990904.1 | 0.0e+00 | 84.46 | probable RNA-dependent RNA polymerase 1 [Cucurbita maxima] | [more] |
| KAG7032582.1 | 0.0e+00 | 84.14 | RNA-dependent RNA polymerase 1, partial [Cucurbita argyrosperma subsp. argyrospe... | [more] |
| XP_023524039.1 | 0.0e+00 | 83.78 | probable RNA-dependent RNA polymerase 1 isoform X1 [Cucurbita pepo subsp. pepo] | [more] |
| KAG6601883.1 | 0.0e+00 | 84.12 | RNA-dependent RNA polymerase 1, partial [Cucurbita argyrosperma subsp. sororia] | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| Q9LQV2 | 0.0e+00 | 57.83 | RNA-dependent RNA polymerase 1 OS=Arabidopsis thaliana OX=3702 GN=RDR1 PE=2 SV=1 | [more] |
| Q0DXS3 | 2.4e-265 | 63.07 | Probable RNA-dependent RNA polymerase 1 OS=Oryza sativa subsp. japonica OX=39947... | [more] |
| O82504 | 1.2e-219 | 39.49 | RNA-dependent RNA polymerase 2 OS=Arabidopsis thaliana OX=3702 GN=RDR2 PE=1 SV=1 | [more] |
| Q7XM31 | 1.5e-214 | 41.20 | Probable RNA-dependent RNA polymerase 2 OS=Oryza sativa subsp. japonica OX=39947... | [more] |
| Q9SG02 | 1.5e-166 | 37.20 | RNA-dependent RNA polymerase 6 OS=Arabidopsis thaliana OX=3702 GN=RDR6 PE=1 SV=1 | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A6J1JTB1 | 0.0e+00 | 84.46 | RNA-dependent RNA polymerase OS=Cucurbita maxima OX=3661 GN=LOC111487656 PE=3 SV... | [more] |
| A0A6J1EB47 | 0.0e+00 | 84.03 | RNA-dependent RNA polymerase OS=Cucurbita moschata OX=3662 GN=LOC111430971 PE=3 ... | [more] |
| A0A6J1FF44 | 0.0e+00 | 83.77 | RNA-dependent RNA polymerase OS=Cucurbita moschata OX=3662 GN=LOC111444909 PE=3 ... | [more] |
| A0A6J1K1Q3 | 0.0e+00 | 83.91 | RNA-dependent RNA polymerase OS=Cucurbita maxima OX=3661 GN=LOC111489826 PE=3 SV... | [more] |
| A0A0S1YCZ4 | 0.0e+00 | 81.97 | RNA-dependent RNA polymerase OS=Cucumis sativus OX=3659 GN=RDR1c PE=2 SV=1 | [more] |
