Spg008512 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg008512
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionTetratricopeptide repeat (TPR)-like superfamily protein
Locationscaffold10: 32961301 .. 32962443 (+)
RNA-Seq ExpressionSpg008512
SyntenySpg008512
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGCTTCCCAGAATCACGCTTGCTAATCAAAGCCTTTGCAAATCCGTCAATTTCACTAATTCTGTCGCTGTTGTTTCACTCATCTTCAGCGAAAATCTCTCTCCCGTTGTTGCTTCTCAATCTTACAGGCCAATTTGTACAGAAGTGATCAATGTTATTCCTCCTCTTGACGAAACCCATATCAGTAATAACTTCATTTCCCTCTTCAGCCAACGAAAATTTTCCCCAGATGACCCTGAGCTCAAAATTTTAGCTCCAAGACTCAACACCCAAATCGTTGAAACTGTATTGAATGGCCTGAGGAATTGGAAGGTTGCTCATATGTTCTTCATTTGGGCCTCAAAACAGCATGGTTACAGGCATAATTGCTATACTTTCAATGCCATTGCATCAATCCTATCACATGCTCGACAAAATGCCCCACTGAGAGCCATTGCTATGGATGTCCTTAACTTTCGTTGTTTGATGACTCCTGGAGCTTTAGGAGTCTTTTTGAGATGTTTGGGAAGCGTGGGGCTGGTTGAGGAAGCTAGCTTTTTGTTTGATCAGGTCAGAGTAATGGGTCTTTGTGTTCCAAATAGTTATAGTTATAACTGTTTGTTGGAAATTTTGTCCAAGGCAAATGCTGTTGATTCCATGGAGAACAGGCTGAGGGAGATGAAAGATTTCGGATGGGAAGTTGATAAGTACACGTTGACACCAGTTTTGAAGGCTTACTGCAATGCTGGGAAGTTTGACAAAGCTTTAAATGTTTATAATGATATTCATGAGAGAGGGTGGGTTGATGGGTACGTCTTTTCTATCTTGGCATTAGCTTTTAGCAAGTGGGGTGAGGTAGGTAGAGCAATGAAATTCATAAACATAATGGGAGATGAAAATCTTGGGTTGAATGAAAAGACATTTTATGCATTGATTCATGGTTTTGTGAAGGAATCCAGGGAGGATATGGCTCTTAAGTTGCTTGAGAAAATGCAGAAACTGGGTTTTACTCCTGATATTTCAATCTATGATGTGCTAATAGGAGGACTTTGTAAGAAGGGAGCATTTGAGAAAACAATGGCTTTGTTTTGGAAGATGAAGTTGCTCGGAATTCCGCCTGACATCGAGATACTTGCAAAGCTGATAGCATCTTCTTCTTAA

mRNA sequence

ATGGCGCTTCCCAGAATCACGCTTGCTAATCAAAGCCTTTGCAAATCCGTCAATTTCACTAATTCTGTCGCTGTTGTTTCACTCATCTTCAGCGAAAATCTCTCTCCCGTTGTTGCTTCTCAATCTTACAGGCCAATTTGTACAGAAGTGATCAATGTTATTCCTCCTCTTGACGAAACCCATATCAGTAATAACTTCATTTCCCTCTTCAGCCAACGAAAATTTTCCCCAGATGACCCTGAGCTCAAAATTTTAGCTCCAAGACTCAACACCCAAATCGTTGAAACTGTATTGAATGGCCTGAGGAATTGGAAGGTTGCTCATATGTTCTTCATTTGGGCCTCAAAACAGCATGGTTACAGGCATAATTGCTATACTTTCAATGCCATTGCATCAATCCTATCACATGCTCGACAAAATGCCCCACTGAGAGCCATTGCTATGGATGTCCTTAACTTTCGTTGTTTGATGACTCCTGGAGCTTTAGGAGTCTTTTTGAGATGTTTGGGAAGCGTGGGGCTGGTTGAGGAAGCTAGCTTTTTGTTTGATCAGGTCAGAGTAATGGGTCTTTGTGTTCCAAATAGTTATAGTTATAACTGTTTGTTGGAAATTTTGTCCAAGGCAAATGCTGTTGATTCCATGGAGAACAGGCTGAGGGAGATGAAAGATTTCGGATGGGAAGTTGATAAGTACACGTTGACACCAGTTTTGAAGGCTTACTGCAATGCTGGGAAGTTTGACAAAGCTTTAAATGTTTATAATGATATTCATGAGAGAGGGTGGGTTGATGGGTACGTCTTTTCTATCTTGGCATTAGCTTTTAGCAAGTGGGGTGAGGTAGGTAGAGCAATGAAATTCATAAACATAATGGGAGATGAAAATCTTGGGTTGAATGAAAAGACATTTTATGCATTGATTCATGGTTTTGTGAAGGAATCCAGGGAGGATATGGCTCTTAAGTTGCTTGAGAAAATGCAGAAACTGGGTTTTACTCCTGATATTTCAATCTATGATGTGCTAATAGGAGGACTTTGTAAGAAGGGAGCATTTGAGAAAACAATGGCTTTGTTTTGGAAGATGAAGTTGCTCGGAATTCCGCCTGACATCGAGATACTTGCAAAGCTGATAGCATCTTCTTCTTAA

Coding sequence (CDS)

ATGGCGCTTCCCAGAATCACGCTTGCTAATCAAAGCCTTTGCAAATCCGTCAATTTCACTAATTCTGTCGCTGTTGTTTCACTCATCTTCAGCGAAAATCTCTCTCCCGTTGTTGCTTCTCAATCTTACAGGCCAATTTGTACAGAAGTGATCAATGTTATTCCTCCTCTTGACGAAACCCATATCAGTAATAACTTCATTTCCCTCTTCAGCCAACGAAAATTTTCCCCAGATGACCCTGAGCTCAAAATTTTAGCTCCAAGACTCAACACCCAAATCGTTGAAACTGTATTGAATGGCCTGAGGAATTGGAAGGTTGCTCATATGTTCTTCATTTGGGCCTCAAAACAGCATGGTTACAGGCATAATTGCTATACTTTCAATGCCATTGCATCAATCCTATCACATGCTCGACAAAATGCCCCACTGAGAGCCATTGCTATGGATGTCCTTAACTTTCGTTGTTTGATGACTCCTGGAGCTTTAGGAGTCTTTTTGAGATGTTTGGGAAGCGTGGGGCTGGTTGAGGAAGCTAGCTTTTTGTTTGATCAGGTCAGAGTAATGGGTCTTTGTGTTCCAAATAGTTATAGTTATAACTGTTTGTTGGAAATTTTGTCCAAGGCAAATGCTGTTGATTCCATGGAGAACAGGCTGAGGGAGATGAAAGATTTCGGATGGGAAGTTGATAAGTACACGTTGACACCAGTTTTGAAGGCTTACTGCAATGCTGGGAAGTTTGACAAAGCTTTAAATGTTTATAATGATATTCATGAGAGAGGGTGGGTTGATGGGTACGTCTTTTCTATCTTGGCATTAGCTTTTAGCAAGTGGGGTGAGGTAGGTAGAGCAATGAAATTCATAAACATAATGGGAGATGAAAATCTTGGGTTGAATGAAAAGACATTTTATGCATTGATTCATGGTTTTGTGAAGGAATCCAGGGAGGATATGGCTCTTAAGTTGCTTGAGAAAATGCAGAAACTGGGTTTTACTCCTGATATTTCAATCTATGATGTGCTAATAGGAGGACTTTGTAAGAAGGGAGCATTTGAGAAAACAATGGCTTTGTTTTGGAAGATGAAGTTGCTCGGAATTCCGCCTGACATCGAGATACTTGCAAAGCTGATAGCATCTTCTTCTTAA

Protein sequence

MALPRITLANQSLCKSVNFTNSVAVVSLIFSENLSPVVASQSYRPICTEVINVIPPLDETHISNNFISLFSQRKFSPDDPELKILAPRLNTQIVETVLNGLRNWKVAHMFFIWASKQHGYRHNCYTFNAIASILSHARQNAPLRAIAMDVLNFRCLMTPGALGVFLRCLGSVGLVEEASFLFDQVRVMGLCVPNSYSYNCLLEILSKANAVDSMENRLREMKDFGWEVDKYTLTPVLKAYCNAGKFDKALNVYNDIHERGWVDGYVFSILALAFSKWGEVGRAMKFINIMGDENLGLNEKTFYALIHGFVKESREDMALKLLEKMQKLGFTPDISIYDVLIGGLCKKGAFEKTMALFWKMKLLGIPPDIEILAKLIASSS
Homology
BLAST of Spg008512 vs. NCBI nr
Match: XP_022141713.1 (putative pentatricopeptide repeat-containing protein At5g08310, mitochondrial [Momordica charantia])

HSP 1 Score: 679.5 bits (1752), Expect = 1.7e-191
Identity = 337/380 (88.68%), Postives = 357/380 (93.95%), Query Frame = 0

Query: 1   MALPRITLANQSLCKSVNFTNSVAVVSLIFSENLSPVVASQSYRPICTEVINVIPPLDET 60
           MALPRITLANQ+LCKS+NFTNSVA+ SLIF E+L PVV  +SYRPICTEVINV+PPLDET
Sbjct: 1   MALPRITLANQNLCKSLNFTNSVAIASLIFCESLPPVV--KSYRPICTEVINVLPPLDET 60

Query: 61  HISNNFISLFSQRKFSPDDPELKILAPRLNTQIVETVLNGLRNWKVAHMFFIWASKQHGY 120
           HISNNFISLFSQRKFSPDDPELKILAPRLNTQIVETVLNGLR+WK+AHMFFIWASKQ+GY
Sbjct: 61  HISNNFISLFSQRKFSPDDPELKILAPRLNTQIVETVLNGLRSWKIAHMFFIWASKQNGY 120

Query: 121 RHNCYTFNAIASILSHARQNAPLRAIAMDVLNFRCLMTPGALGVFLRCLGSVGLVEEASF 180
           RHNCYT+NAIASILS ARQNAPLRA+AMDVLNFRC MTPGALGVFLRCLGSVGLVEEA+F
Sbjct: 121 RHNCYTYNAIASILSRARQNAPLRAVAMDVLNFRCSMTPGALGVFLRCLGSVGLVEEANF 180

Query: 181 LFDQVRVMGLCVPNSYSYNCLLEILSKANAVDSMENRLREMKDFGWEVDKYTLTPVLKAY 240
           LFDQVR MGLCVPNSYSYNCLLEILSKAN+VDS+E RL EMK  G EVDKYTLTPVLKAY
Sbjct: 181 LFDQVRAMGLCVPNSYSYNCLLEILSKANSVDSIETRLSEMKYCGLEVDKYTLTPVLKAY 240

Query: 241 CNAGKFDKALNVYNDIHERGWVDGYVFSILALAFSKWGEVGRAMKFINIMGDENLGLNEK 300
            N+GKFDKALNVYND+HERGWVDGY FSILALAFSKWGEV RAM+FI+ MGD+N GLNEK
Sbjct: 241 FNSGKFDKALNVYNDMHERGWVDGYAFSILALAFSKWGEVDRAMEFIDRMGDQNPGLNEK 300

Query: 301 TFYALIHGFVKESREDMALKLLEKMQKLGFTPDISIYDVLIGGLCKKGAFEKTMALFWKM 360
           TFYALIHGFVKESREDMALKLLEKMQKLGF+PDISIYDVLIG LCKKG FEK MALFWKM
Sbjct: 301 TFYALIHGFVKESREDMALKLLEKMQKLGFSPDISIYDVLIGALCKKGTFEKAMALFWKM 360

Query: 361 KLLGIPPDIEILAKLIASSS 381
           KLLGI PDIEILAKLIAS S
Sbjct: 361 KLLGIVPDIEILAKLIASCS 378

BLAST of Spg008512 vs. NCBI nr
Match: XP_022990710.1 (putative pentatricopeptide repeat-containing protein At5g08310, mitochondrial isoform X3 [Cucurbita maxima])

HSP 1 Score: 669.1 bits (1725), Expect = 2.3e-188
Identity = 331/380 (87.11%), Postives = 350/380 (92.11%), Query Frame = 0

Query: 1   MALPRITLANQSLCKSVNFTNSVAVVSLIFSENLSPVVASQSYRPICTEVINVIPPLDET 60
           MAL RIT ANQSL KSVNFTNSVA +S +FSE+LSPVV SQSYRPIC++ INVIP  DE+
Sbjct: 1   MALSRITFANQSLWKSVNFTNSVAFIS-VFSESLSPVVVSQSYRPICSDAINVIPSHDES 60

Query: 61  HISNNFISLFSQRKFSPDDPELKILAPRLNTQIVETVLNGLRNWKVAHMFFIWASKQHGY 120
           HISNNFISLFSQRK SPDDPELKILAPRLNTQIVE VLNGLRNWKVAHMFFIWASKQHGY
Sbjct: 61  HISNNFISLFSQRKISPDDPELKILAPRLNTQIVENVLNGLRNWKVAHMFFIWASKQHGY 120

Query: 121 RHNCYTFNAIASILSHARQNAPLRAIAMDVLNFRCLMTPGALGVFLRCLGSVGLVEEASF 180
           RHNCYTFN IASILSHARQNAPLRAIAMDV+N RC MTPGALG+FLRCLGSVGLVEEA+F
Sbjct: 121 RHNCYTFNVIASILSHARQNAPLRAIAMDVINSRCSMTPGALGIFLRCLGSVGLVEEANF 180

Query: 181 LFDQVRVMGLCVPNSYSYNCLLEILSKANAVDSMENRLREMKDFGWEVDKYTLTPVLKAY 240
           LFDQVRVMGLCVPNSY+YNCLLEILSKANA+DS+ENRLREMK +G EVDKYTLTPVLKAY
Sbjct: 181 LFDQVRVMGLCVPNSYTYNCLLEILSKANAIDSIENRLREMKYYGCEVDKYTLTPVLKAY 240

Query: 241 CNAGKFDKALNVYNDIHERGWVDGYVFSILALAFSKWGEVGRAMKFINIMGDENLGLNEK 300
           CN GKFDKALNVYNDIHERGW+DGYVFSIL LAFSKWGEV RAM+ I   GD+N  L EK
Sbjct: 241 CNTGKFDKALNVYNDIHERGWIDGYVFSILVLAFSKWGEVDRAMELIERTGDQNPWLTEK 300

Query: 301 TFYALIHGFVKESREDMALKLLEKMQKLGFTPDISIYDVLIGGLCKKGAFEKTMALFWKM 360
           TFYALIHGFVKESREDMA+KLLEKM+KLGF PDISIYDVLIGGLCKKG+FEK M LFWKM
Sbjct: 301 TFYALIHGFVKESREDMAIKLLEKMKKLGFPPDISIYDVLIGGLCKKGSFEKAMTLFWKM 360

Query: 361 KLLGIPPDIEILAKLIASSS 381
           KLLGI PDIEILAKLIASSS
Sbjct: 361 KLLGITPDIEILAKLIASSS 379

BLAST of Spg008512 vs. NCBI nr
Match: XP_022964314.1 (putative pentatricopeptide repeat-containing protein At5g08310, mitochondrial isoform X2 [Cucurbita moschata])

HSP 1 Score: 669.1 bits (1725), Expect = 2.3e-188
Identity = 330/380 (86.84%), Postives = 349/380 (91.84%), Query Frame = 0

Query: 1   MALPRITLANQSLCKSVNFTNSVAVVSLIFSENLSPVVASQSYRPICTEVINVIPPLDET 60
           MAL RIT ANQSL KSVNFTNSVA +S+IF ENLS VV S+SYRPIC+E INV+P  DE+
Sbjct: 1   MALSRITFANQSLWKSVNFTNSVAFISVIFGENLSSVVVSKSYRPICSEAINVLPSHDES 60

Query: 61  HISNNFISLFSQRKFSPDDPELKILAPRLNTQIVETVLNGLRNWKVAHMFFIWASKQHGY 120
           HISNNFISLFSQ KFSPDDPELKILAPRLNTQIVE VLNGLRNWKVAHMFFIWASKQHGY
Sbjct: 61  HISNNFISLFSQLKFSPDDPELKILAPRLNTQIVENVLNGLRNWKVAHMFFIWASKQHGY 120

Query: 121 RHNCYTFNAIASILSHARQNAPLRAIAMDVLNFRCLMTPGALGVFLRCLGSVGLVEEASF 180
           RHNCYTFN IASILSHARQNAPLRAIA DVLN RC MTPGALG+FLRCLGSVGLVEEA+F
Sbjct: 121 RHNCYTFNVIASILSHARQNAPLRAIATDVLNSRCSMTPGALGIFLRCLGSVGLVEEANF 180

Query: 181 LFDQVRVMGLCVPNSYSYNCLLEILSKANAVDSMENRLREMKDFGWEVDKYTLTPVLKAY 240
           LFDQVRVMGLCVPNSY+YNCLLEILSKANA+DS+ENRLREMK +G EVDKYTLTPVL+AY
Sbjct: 181 LFDQVRVMGLCVPNSYTYNCLLEILSKANAIDSIENRLREMKYYGCEVDKYTLTPVLRAY 240

Query: 241 CNAGKFDKALNVYNDIHERGWVDGYVFSILALAFSKWGEVGRAMKFINIMGDENLGLNEK 300
           CNAGKFDKALNVYNDIHERGW+DGYVFSIL LAFSKWGEV RAM+ I   GD+N  L EK
Sbjct: 241 CNAGKFDKALNVYNDIHERGWIDGYVFSILVLAFSKWGEVDRAMELIERTGDQNPWLTEK 300

Query: 301 TFYALIHGFVKESREDMALKLLEKMQKLGFTPDISIYDVLIGGLCKKGAFEKTMALFWKM 360
           TFYALIHGFVKESREDMA+KLLEKM+KLGF PDISIYDVLIGGLCKKG+FEK M LFWKM
Sbjct: 301 TFYALIHGFVKESREDMAIKLLEKMKKLGFPPDISIYDVLIGGLCKKGSFEKAMTLFWKM 360

Query: 361 KLLGIPPDIEILAKLIASSS 381
           KLLGI PDIEILAKLIASSS
Sbjct: 361 KLLGITPDIEILAKLIASSS 380

BLAST of Spg008512 vs. NCBI nr
Match: XP_022990709.1 (putative pentatricopeptide repeat-containing protein At5g08310, mitochondrial isoform X2 [Cucurbita maxima])

HSP 1 Score: 669.1 bits (1725), Expect = 2.3e-188
Identity = 331/380 (87.11%), Postives = 350/380 (92.11%), Query Frame = 0

Query: 1   MALPRITLANQSLCKSVNFTNSVAVVSLIFSENLSPVVASQSYRPICTEVINVIPPLDET 60
           MAL RIT ANQSL KSVNFTNSVA +S +FSE+LSPVV SQSYRPIC++ INVIP  DE+
Sbjct: 1   MALSRITFANQSLWKSVNFTNSVAFIS-VFSESLSPVVVSQSYRPICSDAINVIPSHDES 60

Query: 61  HISNNFISLFSQRKFSPDDPELKILAPRLNTQIVETVLNGLRNWKVAHMFFIWASKQHGY 120
           HISNNFISLFSQRK SPDDPELKILAPRLNTQIVE VLNGLRNWKVAHMFFIWASKQHGY
Sbjct: 61  HISNNFISLFSQRKISPDDPELKILAPRLNTQIVENVLNGLRNWKVAHMFFIWASKQHGY 120

Query: 121 RHNCYTFNAIASILSHARQNAPLRAIAMDVLNFRCLMTPGALGVFLRCLGSVGLVEEASF 180
           RHNCYTFN IASILSHARQNAPLRAIAMDV+N RC MTPGALG+FLRCLGSVGLVEEA+F
Sbjct: 121 RHNCYTFNVIASILSHARQNAPLRAIAMDVINSRCSMTPGALGIFLRCLGSVGLVEEANF 180

Query: 181 LFDQVRVMGLCVPNSYSYNCLLEILSKANAVDSMENRLREMKDFGWEVDKYTLTPVLKAY 240
           LFDQVRVMGLCVPNSY+YNCLLEILSKANA+DS+ENRLREMK +G EVDKYTLTPVLKAY
Sbjct: 181 LFDQVRVMGLCVPNSYTYNCLLEILSKANAIDSIENRLREMKYYGCEVDKYTLTPVLKAY 240

Query: 241 CNAGKFDKALNVYNDIHERGWVDGYVFSILALAFSKWGEVGRAMKFINIMGDENLGLNEK 300
           CN GKFDKALNVYNDIHERGW+DGYVFSIL LAFSKWGEV RAM+ I   GD+N  L EK
Sbjct: 241 CNTGKFDKALNVYNDIHERGWIDGYVFSILVLAFSKWGEVDRAMELIERTGDQNPWLTEK 300

Query: 301 TFYALIHGFVKESREDMALKLLEKMQKLGFTPDISIYDVLIGGLCKKGAFEKTMALFWKM 360
           TFYALIHGFVKESREDMA+KLLEKM+KLGF PDISIYDVLIGGLCKKG+FEK M LFWKM
Sbjct: 301 TFYALIHGFVKESREDMAIKLLEKMKKLGFPPDISIYDVLIGGLCKKGSFEKAMTLFWKM 360

Query: 361 KLLGIPPDIEILAKLIASSS 381
           KLLGI PDIEILAKLIASSS
Sbjct: 361 KLLGITPDIEILAKLIASSS 379

BLAST of Spg008512 vs. NCBI nr
Match: XP_022964306.1 (putative pentatricopeptide repeat-containing protein At5g08310, mitochondrial isoform X1 [Cucurbita moschata])

HSP 1 Score: 669.1 bits (1725), Expect = 2.3e-188
Identity = 330/380 (86.84%), Postives = 349/380 (91.84%), Query Frame = 0

Query: 1   MALPRITLANQSLCKSVNFTNSVAVVSLIFSENLSPVVASQSYRPICTEVINVIPPLDET 60
           MAL RIT ANQSL KSVNFTNSVA +S+IF ENLS VV S+SYRPIC+E INV+P  DE+
Sbjct: 1   MALSRITFANQSLWKSVNFTNSVAFISVIFGENLSSVVVSKSYRPICSEAINVLPSHDES 60

Query: 61  HISNNFISLFSQRKFSPDDPELKILAPRLNTQIVETVLNGLRNWKVAHMFFIWASKQHGY 120
           HISNNFISLFSQ KFSPDDPELKILAPRLNTQIVE VLNGLRNWKVAHMFFIWASKQHGY
Sbjct: 61  HISNNFISLFSQLKFSPDDPELKILAPRLNTQIVENVLNGLRNWKVAHMFFIWASKQHGY 120

Query: 121 RHNCYTFNAIASILSHARQNAPLRAIAMDVLNFRCLMTPGALGVFLRCLGSVGLVEEASF 180
           RHNCYTFN IASILSHARQNAPLRAIA DVLN RC MTPGALG+FLRCLGSVGLVEEA+F
Sbjct: 121 RHNCYTFNVIASILSHARQNAPLRAIATDVLNSRCSMTPGALGIFLRCLGSVGLVEEANF 180

Query: 181 LFDQVRVMGLCVPNSYSYNCLLEILSKANAVDSMENRLREMKDFGWEVDKYTLTPVLKAY 240
           LFDQVRVMGLCVPNSY+YNCLLEILSKANA+DS+ENRLREMK +G EVDKYTLTPVL+AY
Sbjct: 181 LFDQVRVMGLCVPNSYTYNCLLEILSKANAIDSIENRLREMKYYGCEVDKYTLTPVLRAY 240

Query: 241 CNAGKFDKALNVYNDIHERGWVDGYVFSILALAFSKWGEVGRAMKFINIMGDENLGLNEK 300
           CNAGKFDKALNVYNDIHERGW+DGYVFSIL LAFSKWGEV RAM+ I   GD+N  L EK
Sbjct: 241 CNAGKFDKALNVYNDIHERGWIDGYVFSILVLAFSKWGEVDRAMELIERTGDQNPWLTEK 300

Query: 301 TFYALIHGFVKESREDMALKLLEKMQKLGFTPDISIYDVLIGGLCKKGAFEKTMALFWKM 360
           TFYALIHGFVKESREDMA+KLLEKM+KLGF PDISIYDVLIGGLCKKG+FEK M LFWKM
Sbjct: 301 TFYALIHGFVKESREDMAIKLLEKMKKLGFPPDISIYDVLIGGLCKKGSFEKAMTLFWKM 360

Query: 361 KLLGIPPDIEILAKLIASSS 381
           KLLGI PDIEILAKLIASSS
Sbjct: 361 KLLGITPDIEILAKLIASSS 380

BLAST of Spg008512 vs. ExPASy Swiss-Prot
Match: P0C8Q6 (Putative pentatricopeptide repeat-containing protein At5g08310, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At5g08310 PE=3 SV=1)

HSP 1 Score: 392.5 bits (1007), Expect = 5.4e-108
Identity = 185/342 (54.09%), Postives = 263/342 (76.90%), Query Frame = 0

Query: 43  YRPICTEVINV--IPPLDETHISNNFISLFSQRKFSPDDPELKILAPRLNTQIVETVLNG 102
           +RP+ T++ N   + P +++ ++ N I +F+++ FSPDDPEL IL+P LNT++VETVLNG
Sbjct: 24  HRPLTTKLDNTRFLHP-NQSKLAQNLIVIFTRQPFSPDDPELLILSPELNTKVVETVLNG 83

Query: 103 LRNWKVAHMFFIWASKQHGYRHNCYTFNAIASILSHARQNAPLRAIAMDVLNFRCLMTPG 162
            + W +A++FF WASKQ GYR++ Y +NA+ASILS ARQNA L+A+ +DVLN RC M+PG
Sbjct: 84  FKRWGLAYLFFNWASKQEGYRNDMYAYNAMASILSRARQNASLKALVVDVLNSRCFMSPG 143

Query: 163 ALGVFLRCLGSVGLVEEASFLFDQVRVMGLCVPNSYSYNCLLEILSKAN--AVDSMENRL 222
           A G F+RCLG+ GLV+EAS +FD+VR MGLCVPN+Y+YNCLLE +SK+N  +V+ +E RL
Sbjct: 144 AFGFFIRCLGNAGLVDEASSVFDRVREMGLCVPNAYTYNCLLEAISKSNSSSVELVEARL 203

Query: 223 REMKDFGWEVDKYTLTPVLKAYCNAGKFDKALNVYNDIHERGWVDGYVFSILALAFSKWG 282
           +EM+D G+  DK+TLTPVL+ YCN GK ++AL+V+N+I  RGW+D ++ +IL ++F KWG
Sbjct: 204 KEMRDCGFHFDKFTLTPVLQVYCNTGKSERALSVFNEILSRGWLDEHISTILVVSFCKWG 263

Query: 283 EVGRAMKFINIMGDENLGLNEKTFYALIHGFVKESREDMALKLLEKMQKLGFTPDISIYD 342
           +V +A + I ++ + ++ LN KT+  LIHGFVKESR D A +L EKM+++G   DI++YD
Sbjct: 264 QVDKAFELIEMLEERDIRLNYKTYCVLIHGFVKESRIDKAFQLFEKMRRMGMNADIALYD 323

Query: 343 VLIGGLCKKGAFEKTMALFWKMKLLGIPPDIEILAKLIASSS 381
           VLIGGLCK    E  ++L+ ++K  GIPPD  IL KL+ S S
Sbjct: 324 VLIGGLCKHKDLEMALSLYLEIKRSGIPPDRGILGKLLCSFS 364

BLAST of Spg008512 vs. ExPASy Swiss-Prot
Match: Q9LR67 (Pentatricopeptide repeat-containing protein At1g03560, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At1g03560 PE=2 SV=1)

HSP 1 Score: 122.5 bits (306), Expect = 1.0e-26
Identity = 73/265 (27.55%), Postives = 126/265 (47.55%), Query Frame = 0

Query: 106 VAHMFFIWASKQHGYRHNCYTFNAIASILSHARQNAPLRAIAMDVLNFRCLMTPGALGVF 165
           +A  FF W+ KQ  Y HN   + ++  +L+ A+    +R ++ ++  F   MT  A    
Sbjct: 134 IAWSFFCWSRKQKKYTHNLECYVSLVDVLALAKDVDRIRFVSSEIKKFEFPMTVSAANAL 193

Query: 166 LRCLGSVGLVEEASFLFDQVRVMGLCVPNSYSYNCLLEILSKANAVDSMENRLREMKDFG 225
           ++  G +G+VEE  +++ +++  G+  P  Y+YN L+  L  A  VDS E     M+   
Sbjct: 194 IKSFGKLGMVEELLWVWRKMKENGI-EPTLYTYNFLMNGLVSAMFVDSAERVFEVMESGR 253

Query: 226 WEVDKYTLTPVLKAYCNAGKFDKALNVYNDIHERGW-VDGYVFSILALAFSKWGEVGRAM 285
            + D  T   ++K YC AG+  KA+    D+  RG   D   +  +  A     + G  +
Sbjct: 254 IKPDIVTYNTMIKGYCKAGQTQKAMEKLRDMETRGHEADKITYMTMIQACYADSDFGSCV 313

Query: 286 KFINIMGDENLGLNEKTFYALIHGFVKESREDMALKLLEKMQKLGFTPDISIYDVLIGGL 345
                M ++ + +    F  +I G  KE + +    + E M + G  P+++IY VLI G 
Sbjct: 314 ALYQEMDEKGIQVPPHAFSLVIGGLCKEGKLNEGYTVFENMIRKGSKPNVAIYTVLIDGY 373

Query: 346 CKKGAFEKTMALFWKMKLLGIPPDI 370
            K G+ E  + L  +M   G  PD+
Sbjct: 374 AKSGSVEDAIRLLHRMIDEGFKPDV 397

BLAST of Spg008512 vs. ExPASy Swiss-Prot
Match: Q9SAD9 (Pentatricopeptide repeat-containing protein At1g13040, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At1g13040 PE=2 SV=1)

HSP 1 Score: 103.6 bits (257), Expect = 5.0e-21
Identity = 75/276 (27.17%), Postives = 126/276 (45.65%), Query Frame = 0

Query: 97  VLNGL-RNWKVAHMFFIW-ASKQHGYRHNCYTFNAIASILSHARQ-NAPLRAIAMDVLNF 156
           ++NGL R  KV     IW A  + G   +     A+   L HAR+ +     +A ++ + 
Sbjct: 155 LINGLFRAGKVTDAVEIWNAMIRSGVSPDNKACAALVVGLCHARKVDLAYEMVAEEIKSA 214

Query: 157 RCLMTPGALGVFLRCLGSVGLVEEASFLFDQVRVMGLCVPNSYSYNCLLEILSKANAVDS 216
           R  ++       +      G +E+A  L   +  +G C P+  +YN LL      N +  
Sbjct: 215 RVKLSTVVYNALISGFCKAGRIEKAEALKSYMSKIG-CEPDLVTYNVLLNYYYDNNMLKR 274

Query: 217 MENRLREMKDFGWEVDKYTLTPVLKAYCNAGKFDKALN-VYNDIHERGWVDGYVFSILAL 276
            E  + EM   G ++D Y+   +LK +C     DK  N +  ++  RG+ D   +S L  
Sbjct: 275 AEGVMAEMVRSGIQLDAYSYNQLLKRHCRVSHPDKCYNFMVKEMEPRGFCDVVSYSTLIE 334

Query: 277 AFSKWGEVGRAMKFINIMGDENLGLNEKTFYALIHGFVKESREDMALKLLEKMQKLGFTP 336
            F +     +A +    M  + + +N  T+ +LI  F++E    +A KLL++M +LG +P
Sbjct: 335 TFCRASNTRKAYRLFEEMRQKGMVMNVVTYTSLIKAFLREGNSSVAKKLLDQMTELGLSP 394

Query: 337 DISIYDVLIGGLCKKGAFEKTMALFWKMKLLGIPPD 369
           D   Y  ++  LCK G  +K   +F  M    I PD
Sbjct: 395 DRIFYTTILDHLCKSGNVDKAYGVFNDMIEHEITPD 429

BLAST of Spg008512 vs. ExPASy Swiss-Prot
Match: Q9SAJ5 (Pentatricopeptide repeat-containing protein At1g79540 OS=Arabidopsis thaliana OX=3702 GN=At1g79540 PE=2 SV=1)

HSP 1 Score: 101.7 bits (252), Expect = 1.9e-20
Identity = 85/346 (24.57%), Postives = 147/346 (42.49%), Query Frame = 0

Query: 59  ETHISNNFISLFSQRKFSPDDPELKILAPRLNTQIVETVLNGLRNWKVAHMFFIWASKQH 118
           E +IS   IS+ +++K  P +P L+ L P L+  I+ +V+    N ++   FFIWAS++ 
Sbjct: 30  EFNISGEVISILAKKK--PIEPALEPLVPFLSKNIITSVIKDEVNRQLGFRFFIWASRRE 89

Query: 119 GYRHNCYTFNAIASILS---------HARQNAPLRAIAMDVLNFRCLMTPGA-LGVFLRC 178
             R    +F  +  +LS            +      +++D   F  L++  A +G+  + 
Sbjct: 90  RLRSR-ESFGLVIDMLSEDNGCDLYWQTLEELKSGGVSVDSYCFCVLISAYAKMGMAEKA 149

Query: 179 LGSVGLVEEAS-----FLFDQV-RVMGL-------------------CVPNSYSYNCLLE 238
           + S G ++E       F ++ + RVM                     C PN Y++  L++
Sbjct: 150 VESFGRMKEFDCRPDVFTYNVILRVMMREEVFFMLAFAVYNEMLKCNCSPNLYTFGILMD 209

Query: 239 ILSKANAVDSMENRLREMKDFGWEVDKYTLTPVLKAYCNAGKFDKALNVYNDIHERG-WV 298
            L K       +    +M   G   ++ T T ++   C  G  D A  ++ ++   G + 
Sbjct: 210 GLYKKGRTSDAQKMFDDMTGRGISPNRVTYTILISGLCQRGSADDARKLFYEMQTSGNYP 269

Query: 299 DGYVFSILALAFSKWGEVGRAMKFINIMGDENLGLNEKTFYALIHGFVKESREDMALKLL 358
           D    + L   F K G +  A + + +   +   L  + + +LI G  +  R   A +L 
Sbjct: 270 DSVAHNALLDGFCKLGRMVEAFELLRLFEKDGFVLGLRGYSSLIDGLFRARRYTQAFELY 329

Query: 359 EKMQKLGFTPDISIYDVLIGGLCKKGAFEKTMALFWKMKLLGIPPD 369
             M K    PDI +Y +LI GL K G  E  + L   M   GI PD
Sbjct: 330 ANMLKKNIKPDIILYTILIQGLSKAGKIEDALKLLSSMPSKGISPD 372

BLAST of Spg008512 vs. ExPASy Swiss-Prot
Match: Q9FMF6 (Pentatricopeptide repeat-containing protein At5g64320, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At5g64320 PE=2 SV=1)

HSP 1 Score: 99.8 bits (247), Expect = 7.3e-20
Identity = 63/206 (30.58%), Postives = 98/206 (47.57%), Query Frame = 0

Query: 172 VGLVEEASFLFDQVRVMGLCVPNSYSYNCLLEILSKANAVDSMENRLREMKDFGWEVDKY 231
           +G ++EA  + +++   GL  PN+  +NCL+    K + +       REM   G + D Y
Sbjct: 437 LGKIDEAYNVLNEMSADGL-KPNTVGFNCLISAFCKEHRIPEAVEIFREMPRKGCKPDVY 496

Query: 232 TLTPVLKAYCNAGKFDKALNVYNDIHERGWVDGYV-FSILALAFSKWGEVGRAMKFINIM 291
           T   ++   C   +   AL +  D+   G V   V ++ L  AF + GE+  A K +N M
Sbjct: 497 TFNSLISGLCEVDEIKHALWLLRDMISEGVVANTVTYNTLINAFLRRGEIKEARKLVNEM 556

Query: 292 GDENLGLNEKTFYALIHGFVKESREDMALKLLEKMQKLGFTPDISIYDVLIGGLCKKGAF 351
             +   L+E T+ +LI G  +    D A  L EKM + G  P     ++LI GLC+ G  
Sbjct: 557 VFQGSPLDEITYNSLIKGLCRAGEVDKARSLFEKMLRDGHAPSNISCNILINGLCRSGMV 616

Query: 352 EKTMALFWKMKLLGIPPDIEILAKLI 377
           E+ +    +M L G  PDI     LI
Sbjct: 617 EEAVEFQKEMVLRGSTPDIVTFNSLI 641

BLAST of Spg008512 vs. ExPASy TrEMBL
Match: A0A6J1CLB5 (putative pentatricopeptide repeat-containing protein At5g08310, mitochondrial OS=Momordica charantia OX=3673 GN=LOC111012005 PE=3 SV=1)

HSP 1 Score: 679.5 bits (1752), Expect = 8.2e-192
Identity = 337/380 (88.68%), Postives = 357/380 (93.95%), Query Frame = 0

Query: 1   MALPRITLANQSLCKSVNFTNSVAVVSLIFSENLSPVVASQSYRPICTEVINVIPPLDET 60
           MALPRITLANQ+LCKS+NFTNSVA+ SLIF E+L PVV  +SYRPICTEVINV+PPLDET
Sbjct: 1   MALPRITLANQNLCKSLNFTNSVAIASLIFCESLPPVV--KSYRPICTEVINVLPPLDET 60

Query: 61  HISNNFISLFSQRKFSPDDPELKILAPRLNTQIVETVLNGLRNWKVAHMFFIWASKQHGY 120
           HISNNFISLFSQRKFSPDDPELKILAPRLNTQIVETVLNGLR+WK+AHMFFIWASKQ+GY
Sbjct: 61  HISNNFISLFSQRKFSPDDPELKILAPRLNTQIVETVLNGLRSWKIAHMFFIWASKQNGY 120

Query: 121 RHNCYTFNAIASILSHARQNAPLRAIAMDVLNFRCLMTPGALGVFLRCLGSVGLVEEASF 180
           RHNCYT+NAIASILS ARQNAPLRA+AMDVLNFRC MTPGALGVFLRCLGSVGLVEEA+F
Sbjct: 121 RHNCYTYNAIASILSRARQNAPLRAVAMDVLNFRCSMTPGALGVFLRCLGSVGLVEEANF 180

Query: 181 LFDQVRVMGLCVPNSYSYNCLLEILSKANAVDSMENRLREMKDFGWEVDKYTLTPVLKAY 240
           LFDQVR MGLCVPNSYSYNCLLEILSKAN+VDS+E RL EMK  G EVDKYTLTPVLKAY
Sbjct: 181 LFDQVRAMGLCVPNSYSYNCLLEILSKANSVDSIETRLSEMKYCGLEVDKYTLTPVLKAY 240

Query: 241 CNAGKFDKALNVYNDIHERGWVDGYVFSILALAFSKWGEVGRAMKFINIMGDENLGLNEK 300
            N+GKFDKALNVYND+HERGWVDGY FSILALAFSKWGEV RAM+FI+ MGD+N GLNEK
Sbjct: 241 FNSGKFDKALNVYNDMHERGWVDGYAFSILALAFSKWGEVDRAMEFIDRMGDQNPGLNEK 300

Query: 301 TFYALIHGFVKESREDMALKLLEKMQKLGFTPDISIYDVLIGGLCKKGAFEKTMALFWKM 360
           TFYALIHGFVKESREDMALKLLEKMQKLGF+PDISIYDVLIG LCKKG FEK MALFWKM
Sbjct: 301 TFYALIHGFVKESREDMALKLLEKMQKLGFSPDISIYDVLIGALCKKGTFEKAMALFWKM 360

Query: 361 KLLGIPPDIEILAKLIASSS 381
           KLLGI PDIEILAKLIAS S
Sbjct: 361 KLLGIVPDIEILAKLIASCS 378

BLAST of Spg008512 vs. ExPASy TrEMBL
Match: A0A6J1JSS2 (putative pentatricopeptide repeat-containing protein At5g08310, mitochondrial isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111487519 PE=4 SV=1)

HSP 1 Score: 669.1 bits (1725), Expect = 1.1e-188
Identity = 331/380 (87.11%), Postives = 350/380 (92.11%), Query Frame = 0

Query: 1   MALPRITLANQSLCKSVNFTNSVAVVSLIFSENLSPVVASQSYRPICTEVINVIPPLDET 60
           MAL RIT ANQSL KSVNFTNSVA +S +FSE+LSPVV SQSYRPIC++ INVIP  DE+
Sbjct: 1   MALSRITFANQSLWKSVNFTNSVAFIS-VFSESLSPVVVSQSYRPICSDAINVIPSHDES 60

Query: 61  HISNNFISLFSQRKFSPDDPELKILAPRLNTQIVETVLNGLRNWKVAHMFFIWASKQHGY 120
           HISNNFISLFSQRK SPDDPELKILAPRLNTQIVE VLNGLRNWKVAHMFFIWASKQHGY
Sbjct: 61  HISNNFISLFSQRKISPDDPELKILAPRLNTQIVENVLNGLRNWKVAHMFFIWASKQHGY 120

Query: 121 RHNCYTFNAIASILSHARQNAPLRAIAMDVLNFRCLMTPGALGVFLRCLGSVGLVEEASF 180
           RHNCYTFN IASILSHARQNAPLRAIAMDV+N RC MTPGALG+FLRCLGSVGLVEEA+F
Sbjct: 121 RHNCYTFNVIASILSHARQNAPLRAIAMDVINSRCSMTPGALGIFLRCLGSVGLVEEANF 180

Query: 181 LFDQVRVMGLCVPNSYSYNCLLEILSKANAVDSMENRLREMKDFGWEVDKYTLTPVLKAY 240
           LFDQVRVMGLCVPNSY+YNCLLEILSKANA+DS+ENRLREMK +G EVDKYTLTPVLKAY
Sbjct: 181 LFDQVRVMGLCVPNSYTYNCLLEILSKANAIDSIENRLREMKYYGCEVDKYTLTPVLKAY 240

Query: 241 CNAGKFDKALNVYNDIHERGWVDGYVFSILALAFSKWGEVGRAMKFINIMGDENLGLNEK 300
           CN GKFDKALNVYNDIHERGW+DGYVFSIL LAFSKWGEV RAM+ I   GD+N  L EK
Sbjct: 241 CNTGKFDKALNVYNDIHERGWIDGYVFSILVLAFSKWGEVDRAMELIERTGDQNPWLTEK 300

Query: 301 TFYALIHGFVKESREDMALKLLEKMQKLGFTPDISIYDVLIGGLCKKGAFEKTMALFWKM 360
           TFYALIHGFVKESREDMA+KLLEKM+KLGF PDISIYDVLIGGLCKKG+FEK M LFWKM
Sbjct: 301 TFYALIHGFVKESREDMAIKLLEKMKKLGFPPDISIYDVLIGGLCKKGSFEKAMTLFWKM 360

Query: 361 KLLGIPPDIEILAKLIASSS 381
           KLLGI PDIEILAKLIASSS
Sbjct: 361 KLLGITPDIEILAKLIASSS 379

BLAST of Spg008512 vs. ExPASy TrEMBL
Match: A0A6J1JQU8 (putative pentatricopeptide repeat-containing protein At5g08310, mitochondrial isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111487519 PE=4 SV=1)

HSP 1 Score: 669.1 bits (1725), Expect = 1.1e-188
Identity = 331/380 (87.11%), Postives = 350/380 (92.11%), Query Frame = 0

Query: 1   MALPRITLANQSLCKSVNFTNSVAVVSLIFSENLSPVVASQSYRPICTEVINVIPPLDET 60
           MAL RIT ANQSL KSVNFTNSVA +S +FSE+LSPVV SQSYRPIC++ INVIP  DE+
Sbjct: 1   MALSRITFANQSLWKSVNFTNSVAFIS-VFSESLSPVVVSQSYRPICSDAINVIPSHDES 60

Query: 61  HISNNFISLFSQRKFSPDDPELKILAPRLNTQIVETVLNGLRNWKVAHMFFIWASKQHGY 120
           HISNNFISLFSQRK SPDDPELKILAPRLNTQIVE VLNGLRNWKVAHMFFIWASKQHGY
Sbjct: 61  HISNNFISLFSQRKISPDDPELKILAPRLNTQIVENVLNGLRNWKVAHMFFIWASKQHGY 120

Query: 121 RHNCYTFNAIASILSHARQNAPLRAIAMDVLNFRCLMTPGALGVFLRCLGSVGLVEEASF 180
           RHNCYTFN IASILSHARQNAPLRAIAMDV+N RC MTPGALG+FLRCLGSVGLVEEA+F
Sbjct: 121 RHNCYTFNVIASILSHARQNAPLRAIAMDVINSRCSMTPGALGIFLRCLGSVGLVEEANF 180

Query: 181 LFDQVRVMGLCVPNSYSYNCLLEILSKANAVDSMENRLREMKDFGWEVDKYTLTPVLKAY 240
           LFDQVRVMGLCVPNSY+YNCLLEILSKANA+DS+ENRLREMK +G EVDKYTLTPVLKAY
Sbjct: 181 LFDQVRVMGLCVPNSYTYNCLLEILSKANAIDSIENRLREMKYYGCEVDKYTLTPVLKAY 240

Query: 241 CNAGKFDKALNVYNDIHERGWVDGYVFSILALAFSKWGEVGRAMKFINIMGDENLGLNEK 300
           CN GKFDKALNVYNDIHERGW+DGYVFSIL LAFSKWGEV RAM+ I   GD+N  L EK
Sbjct: 241 CNTGKFDKALNVYNDIHERGWIDGYVFSILVLAFSKWGEVDRAMELIERTGDQNPWLTEK 300

Query: 301 TFYALIHGFVKESREDMALKLLEKMQKLGFTPDISIYDVLIGGLCKKGAFEKTMALFWKM 360
           TFYALIHGFVKESREDMA+KLLEKM+KLGF PDISIYDVLIGGLCKKG+FEK M LFWKM
Sbjct: 301 TFYALIHGFVKESREDMAIKLLEKMKKLGFPPDISIYDVLIGGLCKKGSFEKAMTLFWKM 360

Query: 361 KLLGIPPDIEILAKLIASSS 381
           KLLGI PDIEILAKLIASSS
Sbjct: 361 KLLGITPDIEILAKLIASSS 379

BLAST of Spg008512 vs. ExPASy TrEMBL
Match: A0A6J1JU29 (putative pentatricopeptide repeat-containing protein At5g08310, mitochondrial isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111487519 PE=4 SV=1)

HSP 1 Score: 669.1 bits (1725), Expect = 1.1e-188
Identity = 331/380 (87.11%), Postives = 350/380 (92.11%), Query Frame = 0

Query: 1   MALPRITLANQSLCKSVNFTNSVAVVSLIFSENLSPVVASQSYRPICTEVINVIPPLDET 60
           MAL RIT ANQSL KSVNFTNSVA +S +FSE+LSPVV SQSYRPIC++ INVIP  DE+
Sbjct: 1   MALSRITFANQSLWKSVNFTNSVAFIS-VFSESLSPVVVSQSYRPICSDAINVIPSHDES 60

Query: 61  HISNNFISLFSQRKFSPDDPELKILAPRLNTQIVETVLNGLRNWKVAHMFFIWASKQHGY 120
           HISNNFISLFSQRK SPDDPELKILAPRLNTQIVE VLNGLRNWKVAHMFFIWASKQHGY
Sbjct: 61  HISNNFISLFSQRKISPDDPELKILAPRLNTQIVENVLNGLRNWKVAHMFFIWASKQHGY 120

Query: 121 RHNCYTFNAIASILSHARQNAPLRAIAMDVLNFRCLMTPGALGVFLRCLGSVGLVEEASF 180
           RHNCYTFN IASILSHARQNAPLRAIAMDV+N RC MTPGALG+FLRCLGSVGLVEEA+F
Sbjct: 121 RHNCYTFNVIASILSHARQNAPLRAIAMDVINSRCSMTPGALGIFLRCLGSVGLVEEANF 180

Query: 181 LFDQVRVMGLCVPNSYSYNCLLEILSKANAVDSMENRLREMKDFGWEVDKYTLTPVLKAY 240
           LFDQVRVMGLCVPNSY+YNCLLEILSKANA+DS+ENRLREMK +G EVDKYTLTPVLKAY
Sbjct: 181 LFDQVRVMGLCVPNSYTYNCLLEILSKANAIDSIENRLREMKYYGCEVDKYTLTPVLKAY 240

Query: 241 CNAGKFDKALNVYNDIHERGWVDGYVFSILALAFSKWGEVGRAMKFINIMGDENLGLNEK 300
           CN GKFDKALNVYNDIHERGW+DGYVFSIL LAFSKWGEV RAM+ I   GD+N  L EK
Sbjct: 241 CNTGKFDKALNVYNDIHERGWIDGYVFSILVLAFSKWGEVDRAMELIERTGDQNPWLTEK 300

Query: 301 TFYALIHGFVKESREDMALKLLEKMQKLGFTPDISIYDVLIGGLCKKGAFEKTMALFWKM 360
           TFYALIHGFVKESREDMA+KLLEKM+KLGF PDISIYDVLIGGLCKKG+FEK M LFWKM
Sbjct: 301 TFYALIHGFVKESREDMAIKLLEKMKKLGFPPDISIYDVLIGGLCKKGSFEKAMTLFWKM 360

Query: 361 KLLGIPPDIEILAKLIASSS 381
           KLLGI PDIEILAKLIASSS
Sbjct: 361 KLLGITPDIEILAKLIASSS 379

BLAST of Spg008512 vs. ExPASy TrEMBL
Match: A0A6J1HIJ6 (putative pentatricopeptide repeat-containing protein At5g08310, mitochondrial isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111464360 PE=4 SV=1)

HSP 1 Score: 669.1 bits (1725), Expect = 1.1e-188
Identity = 330/380 (86.84%), Postives = 349/380 (91.84%), Query Frame = 0

Query: 1   MALPRITLANQSLCKSVNFTNSVAVVSLIFSENLSPVVASQSYRPICTEVINVIPPLDET 60
           MAL RIT ANQSL KSVNFTNSVA +S+IF ENLS VV S+SYRPIC+E INV+P  DE+
Sbjct: 1   MALSRITFANQSLWKSVNFTNSVAFISVIFGENLSSVVVSKSYRPICSEAINVLPSHDES 60

Query: 61  HISNNFISLFSQRKFSPDDPELKILAPRLNTQIVETVLNGLRNWKVAHMFFIWASKQHGY 120
           HISNNFISLFSQ KFSPDDPELKILAPRLNTQIVE VLNGLRNWKVAHMFFIWASKQHGY
Sbjct: 61  HISNNFISLFSQLKFSPDDPELKILAPRLNTQIVENVLNGLRNWKVAHMFFIWASKQHGY 120

Query: 121 RHNCYTFNAIASILSHARQNAPLRAIAMDVLNFRCLMTPGALGVFLRCLGSVGLVEEASF 180
           RHNCYTFN IASILSHARQNAPLRAIA DVLN RC MTPGALG+FLRCLGSVGLVEEA+F
Sbjct: 121 RHNCYTFNVIASILSHARQNAPLRAIATDVLNSRCSMTPGALGIFLRCLGSVGLVEEANF 180

Query: 181 LFDQVRVMGLCVPNSYSYNCLLEILSKANAVDSMENRLREMKDFGWEVDKYTLTPVLKAY 240
           LFDQVRVMGLCVPNSY+YNCLLEILSKANA+DS+ENRLREMK +G EVDKYTLTPVL+AY
Sbjct: 181 LFDQVRVMGLCVPNSYTYNCLLEILSKANAIDSIENRLREMKYYGCEVDKYTLTPVLRAY 240

Query: 241 CNAGKFDKALNVYNDIHERGWVDGYVFSILALAFSKWGEVGRAMKFINIMGDENLGLNEK 300
           CNAGKFDKALNVYNDIHERGW+DGYVFSIL LAFSKWGEV RAM+ I   GD+N  L EK
Sbjct: 241 CNAGKFDKALNVYNDIHERGWIDGYVFSILVLAFSKWGEVDRAMELIERTGDQNPWLTEK 300

Query: 301 TFYALIHGFVKESREDMALKLLEKMQKLGFTPDISIYDVLIGGLCKKGAFEKTMALFWKM 360
           TFYALIHGFVKESREDMA+KLLEKM+KLGF PDISIYDVLIGGLCKKG+FEK M LFWKM
Sbjct: 301 TFYALIHGFVKESREDMAIKLLEKMKKLGFPPDISIYDVLIGGLCKKGSFEKAMTLFWKM 360

Query: 361 KLLGIPPDIEILAKLIASSS 381
           KLLGI PDIEILAKLIASSS
Sbjct: 361 KLLGITPDIEILAKLIASSS 380

BLAST of Spg008512 vs. TAIR 10
Match: AT5G08310.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 392.5 bits (1007), Expect = 3.8e-109
Identity = 185/342 (54.09%), Postives = 263/342 (76.90%), Query Frame = 0

Query: 43  YRPICTEVINV--IPPLDETHISNNFISLFSQRKFSPDDPELKILAPRLNTQIVETVLNG 102
           +RP+ T++ N   + P +++ ++ N I +F+++ FSPDDPEL IL+P LNT++VETVLNG
Sbjct: 24  HRPLTTKLDNTRFLHP-NQSKLAQNLIVIFTRQPFSPDDPELLILSPELNTKVVETVLNG 83

Query: 103 LRNWKVAHMFFIWASKQHGYRHNCYTFNAIASILSHARQNAPLRAIAMDVLNFRCLMTPG 162
            + W +A++FF WASKQ GYR++ Y +NA+ASILS ARQNA L+A+ +DVLN RC M+PG
Sbjct: 84  FKRWGLAYLFFNWASKQEGYRNDMYAYNAMASILSRARQNASLKALVVDVLNSRCFMSPG 143

Query: 163 ALGVFLRCLGSVGLVEEASFLFDQVRVMGLCVPNSYSYNCLLEILSKAN--AVDSMENRL 222
           A G F+RCLG+ GLV+EAS +FD+VR MGLCVPN+Y+YNCLLE +SK+N  +V+ +E RL
Sbjct: 144 AFGFFIRCLGNAGLVDEASSVFDRVREMGLCVPNAYTYNCLLEAISKSNSSSVELVEARL 203

Query: 223 REMKDFGWEVDKYTLTPVLKAYCNAGKFDKALNVYNDIHERGWVDGYVFSILALAFSKWG 282
           +EM+D G+  DK+TLTPVL+ YCN GK ++AL+V+N+I  RGW+D ++ +IL ++F KWG
Sbjct: 204 KEMRDCGFHFDKFTLTPVLQVYCNTGKSERALSVFNEILSRGWLDEHISTILVVSFCKWG 263

Query: 283 EVGRAMKFINIMGDENLGLNEKTFYALIHGFVKESREDMALKLLEKMQKLGFTPDISIYD 342
           +V +A + I ++ + ++ LN KT+  LIHGFVKESR D A +L EKM+++G   DI++YD
Sbjct: 264 QVDKAFELIEMLEERDIRLNYKTYCVLIHGFVKESRIDKAFQLFEKMRRMGMNADIALYD 323

Query: 343 VLIGGLCKKGAFEKTMALFWKMKLLGIPPDIEILAKLIASSS 381
           VLIGGLCK    E  ++L+ ++K  GIPPD  IL KL+ S S
Sbjct: 324 VLIGGLCKHKDLEMALSLYLEIKRSGIPPDRGILGKLLCSFS 364

BLAST of Spg008512 vs. TAIR 10
Match: AT1G03560.1 (Pentatricopeptide repeat (PPR-like) superfamily protein )

HSP 1 Score: 122.5 bits (306), Expect = 7.4e-28
Identity = 73/265 (27.55%), Postives = 126/265 (47.55%), Query Frame = 0

Query: 106 VAHMFFIWASKQHGYRHNCYTFNAIASILSHARQNAPLRAIAMDVLNFRCLMTPGALGVF 165
           +A  FF W+ KQ  Y HN   + ++  +L+ A+    +R ++ ++  F   MT  A    
Sbjct: 134 IAWSFFCWSRKQKKYTHNLECYVSLVDVLALAKDVDRIRFVSSEIKKFEFPMTVSAANAL 193

Query: 166 LRCLGSVGLVEEASFLFDQVRVMGLCVPNSYSYNCLLEILSKANAVDSMENRLREMKDFG 225
           ++  G +G+VEE  +++ +++  G+  P  Y+YN L+  L  A  VDS E     M+   
Sbjct: 194 IKSFGKLGMVEELLWVWRKMKENGI-EPTLYTYNFLMNGLVSAMFVDSAERVFEVMESGR 253

Query: 226 WEVDKYTLTPVLKAYCNAGKFDKALNVYNDIHERGW-VDGYVFSILALAFSKWGEVGRAM 285
            + D  T   ++K YC AG+  KA+    D+  RG   D   +  +  A     + G  +
Sbjct: 254 IKPDIVTYNTMIKGYCKAGQTQKAMEKLRDMETRGHEADKITYMTMIQACYADSDFGSCV 313

Query: 286 KFINIMGDENLGLNEKTFYALIHGFVKESREDMALKLLEKMQKLGFTPDISIYDVLIGGL 345
                M ++ + +    F  +I G  KE + +    + E M + G  P+++IY VLI G 
Sbjct: 314 ALYQEMDEKGIQVPPHAFSLVIGGLCKEGKLNEGYTVFENMIRKGSKPNVAIYTVLIDGY 373

Query: 346 CKKGAFEKTMALFWKMKLLGIPPDI 370
            K G+ E  + L  +M   G  PD+
Sbjct: 374 AKSGSVEDAIRLLHRMIDEGFKPDV 397

BLAST of Spg008512 vs. TAIR 10
Match: AT1G13040.1 (Pentatricopeptide repeat (PPR-like) superfamily protein )

HSP 1 Score: 103.6 bits (257), Expect = 3.6e-22
Identity = 75/276 (27.17%), Postives = 126/276 (45.65%), Query Frame = 0

Query: 97  VLNGL-RNWKVAHMFFIW-ASKQHGYRHNCYTFNAIASILSHARQ-NAPLRAIAMDVLNF 156
           ++NGL R  KV     IW A  + G   +     A+   L HAR+ +     +A ++ + 
Sbjct: 155 LINGLFRAGKVTDAVEIWNAMIRSGVSPDNKACAALVVGLCHARKVDLAYEMVAEEIKSA 214

Query: 157 RCLMTPGALGVFLRCLGSVGLVEEASFLFDQVRVMGLCVPNSYSYNCLLEILSKANAVDS 216
           R  ++       +      G +E+A  L   +  +G C P+  +YN LL      N +  
Sbjct: 215 RVKLSTVVYNALISGFCKAGRIEKAEALKSYMSKIG-CEPDLVTYNVLLNYYYDNNMLKR 274

Query: 217 MENRLREMKDFGWEVDKYTLTPVLKAYCNAGKFDKALN-VYNDIHERGWVDGYVFSILAL 276
            E  + EM   G ++D Y+   +LK +C     DK  N +  ++  RG+ D   +S L  
Sbjct: 275 AEGVMAEMVRSGIQLDAYSYNQLLKRHCRVSHPDKCYNFMVKEMEPRGFCDVVSYSTLIE 334

Query: 277 AFSKWGEVGRAMKFINIMGDENLGLNEKTFYALIHGFVKESREDMALKLLEKMQKLGFTP 336
            F +     +A +    M  + + +N  T+ +LI  F++E    +A KLL++M +LG +P
Sbjct: 335 TFCRASNTRKAYRLFEEMRQKGMVMNVVTYTSLIKAFLREGNSSVAKKLLDQMTELGLSP 394

Query: 337 DISIYDVLIGGLCKKGAFEKTMALFWKMKLLGIPPD 369
           D   Y  ++  LCK G  +K   +F  M    I PD
Sbjct: 395 DRIFYTTILDHLCKSGNVDKAYGVFNDMIEHEITPD 429

BLAST of Spg008512 vs. TAIR 10
Match: AT1G79540.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 101.7 bits (252), Expect = 1.4e-21
Identity = 85/346 (24.57%), Postives = 147/346 (42.49%), Query Frame = 0

Query: 59  ETHISNNFISLFSQRKFSPDDPELKILAPRLNTQIVETVLNGLRNWKVAHMFFIWASKQH 118
           E +IS   IS+ +++K  P +P L+ L P L+  I+ +V+    N ++   FFIWAS++ 
Sbjct: 30  EFNISGEVISILAKKK--PIEPALEPLVPFLSKNIITSVIKDEVNRQLGFRFFIWASRRE 89

Query: 119 GYRHNCYTFNAIASILS---------HARQNAPLRAIAMDVLNFRCLMTPGA-LGVFLRC 178
             R    +F  +  +LS            +      +++D   F  L++  A +G+  + 
Sbjct: 90  RLRSR-ESFGLVIDMLSEDNGCDLYWQTLEELKSGGVSVDSYCFCVLISAYAKMGMAEKA 149

Query: 179 LGSVGLVEEAS-----FLFDQV-RVMGL-------------------CVPNSYSYNCLLE 238
           + S G ++E       F ++ + RVM                     C PN Y++  L++
Sbjct: 150 VESFGRMKEFDCRPDVFTYNVILRVMMREEVFFMLAFAVYNEMLKCNCSPNLYTFGILMD 209

Query: 239 ILSKANAVDSMENRLREMKDFGWEVDKYTLTPVLKAYCNAGKFDKALNVYNDIHERG-WV 298
            L K       +    +M   G   ++ T T ++   C  G  D A  ++ ++   G + 
Sbjct: 210 GLYKKGRTSDAQKMFDDMTGRGISPNRVTYTILISGLCQRGSADDARKLFYEMQTSGNYP 269

Query: 299 DGYVFSILALAFSKWGEVGRAMKFINIMGDENLGLNEKTFYALIHGFVKESREDMALKLL 358
           D    + L   F K G +  A + + +   +   L  + + +LI G  +  R   A +L 
Sbjct: 270 DSVAHNALLDGFCKLGRMVEAFELLRLFEKDGFVLGLRGYSSLIDGLFRARRYTQAFELY 329

Query: 359 EKMQKLGFTPDISIYDVLIGGLCKKGAFEKTMALFWKMKLLGIPPD 369
             M K    PDI +Y +LI GL K G  E  + L   M   GI PD
Sbjct: 330 ANMLKKNIKPDIILYTILIQGLSKAGKIEDALKLLSSMPSKGISPD 372

BLAST of Spg008512 vs. TAIR 10
Match: AT5G64320.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 99.8 bits (247), Expect = 5.2e-21
Identity = 63/206 (30.58%), Postives = 98/206 (47.57%), Query Frame = 0

Query: 172 VGLVEEASFLFDQVRVMGLCVPNSYSYNCLLEILSKANAVDSMENRLREMKDFGWEVDKY 231
           +G ++EA  + +++   GL  PN+  +NCL+    K + +       REM   G + D Y
Sbjct: 437 LGKIDEAYNVLNEMSADGL-KPNTVGFNCLISAFCKEHRIPEAVEIFREMPRKGCKPDVY 496

Query: 232 TLTPVLKAYCNAGKFDKALNVYNDIHERGWVDGYV-FSILALAFSKWGEVGRAMKFINIM 291
           T   ++   C   +   AL +  D+   G V   V ++ L  AF + GE+  A K +N M
Sbjct: 497 TFNSLISGLCEVDEIKHALWLLRDMISEGVVANTVTYNTLINAFLRRGEIKEARKLVNEM 556

Query: 292 GDENLGLNEKTFYALIHGFVKESREDMALKLLEKMQKLGFTPDISIYDVLIGGLCKKGAF 351
             +   L+E T+ +LI G  +    D A  L EKM + G  P     ++LI GLC+ G  
Sbjct: 557 VFQGSPLDEITYNSLIKGLCRAGEVDKARSLFEKMLRDGHAPSNISCNILINGLCRSGMV 616

Query: 352 EKTMALFWKMKLLGIPPDIEILAKLI 377
           E+ +    +M L G  PDI     LI
Sbjct: 617 EEAVEFQKEMVLRGSTPDIVTFNSLI 641

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022141713.11.7e-19188.68putative pentatricopeptide repeat-containing protein At5g08310, mitochondrial [M... [more]
XP_022990710.12.3e-18887.11putative pentatricopeptide repeat-containing protein At5g08310, mitochondrial is... [more]
XP_022964314.12.3e-18886.84putative pentatricopeptide repeat-containing protein At5g08310, mitochondrial is... [more]
XP_022990709.12.3e-18887.11putative pentatricopeptide repeat-containing protein At5g08310, mitochondrial is... [more]
XP_022964306.12.3e-18886.84putative pentatricopeptide repeat-containing protein At5g08310, mitochondrial is... [more]
Match NameE-valueIdentityDescription
P0C8Q65.4e-10854.09Putative pentatricopeptide repeat-containing protein At5g08310, mitochondrial OS... [more]
Q9LR671.0e-2627.55Pentatricopeptide repeat-containing protein At1g03560, mitochondrial OS=Arabidop... [more]
Q9SAD95.0e-2127.17Pentatricopeptide repeat-containing protein At1g13040, mitochondrial OS=Arabidop... [more]
Q9SAJ51.9e-2024.57Pentatricopeptide repeat-containing protein At1g79540 OS=Arabidopsis thaliana OX... [more]
Q9FMF67.3e-2030.58Pentatricopeptide repeat-containing protein At5g64320, mitochondrial OS=Arabidop... [more]
Match NameE-valueIdentityDescription
A0A6J1CLB58.2e-19288.68putative pentatricopeptide repeat-containing protein At5g08310, mitochondrial OS... [more]
A0A6J1JSS21.1e-18887.11putative pentatricopeptide repeat-containing protein At5g08310, mitochondrial is... [more]
A0A6J1JQU81.1e-18887.11putative pentatricopeptide repeat-containing protein At5g08310, mitochondrial is... [more]
A0A6J1JU291.1e-18887.11putative pentatricopeptide repeat-containing protein At5g08310, mitochondrial is... [more]
A0A6J1HIJ61.1e-18886.84putative pentatricopeptide repeat-containing protein At5g08310, mitochondrial is... [more]
Match NameE-valueIdentityDescription
AT5G08310.13.8e-10954.09Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT1G03560.17.4e-2827.55Pentatricopeptide repeat (PPR-like) superfamily protein [more]
AT1G13040.13.6e-2227.17Pentatricopeptide repeat (PPR-like) superfamily protein [more]
AT1G79540.11.4e-2124.57Pentatricopeptide repeat (PPR) superfamily protein [more]
AT5G64320.15.2e-2130.58Pentatricopeptide repeat (PPR) superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002885Pentatricopeptide repeatPFAMPF13041PPR_2coord: 300..346
e-value: 6.3E-9
score: 35.9
coord: 193..242
e-value: 4.5E-8
score: 33.2
IPR002885Pentatricopeptide repeatTIGRFAMTIGR00756TIGR00756coord: 336..369
e-value: 0.001
score: 17.1
coord: 301..334
e-value: 6.4E-7
score: 27.2
coord: 231..262
e-value: 8.0E-7
score: 26.9
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 229..263
score: 10.39137
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 298..332
score: 11.169622
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 333..367
score: 11.290196
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 194..228
score: 9.086975
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 223..291
e-value: 1.1E-9
score: 40.1
coord: 292..364
e-value: 2.9E-16
score: 61.5
coord: 81..222
e-value: 9.4E-17
score: 63.1
NoneNo IPR availablePANTHERPTHR47938:SF24PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN MITOCHONDRIAL-RELATEDcoord: 54..379
NoneNo IPR availablePANTHERPTHR47938RESPIRATORY COMPLEX I CHAPERONE (CIA84), PUTATIVE (AFU_ORTHOLOGUE AFUA_2G06020)-RELATEDcoord: 54..379

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg008512.1Spg008512.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005515 protein binding