Spg008434 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg008434
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionSASA domain-containing protein
Locationscaffold10: 36448774 .. 36449612 (-)
RNA-Seq ExpressionSpg008434
SyntenySpg008434
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTGGTCGAGGTGGCGTCTCCAAAGACCCAATCACAGGCAAAAATATATGGGACGGATATATCCCACCAGAGTCCCAGTCCAATGAATCAATCTTTCGATTCAATGCTAATTCAGAATGGGAACAAGCCTCTGAACCACTCCATTGGGATATTGATGTTAAAAAAACCAATGGAATCGGACCTGGGATGGCTTTTGCCAATGAGCTTTTGGCCAAAGCTGGTAAGAGCATTGGTGGCATCGGTCTTGTTCCGTGTGCCATAGGAGGAACTCACTTAAGAGAATGGGTTAAAGGGACTGATAGTTACACCAAATTGGTTGAGCGAATTAAAGCTTCAGAAAAACAAGGGGGAAAAGTCCAAGGATTTTTTTGGTATCAAGGAGAGTCTGATGCTGCAGTAGAAGAAGAATCTAAATGCTATGAAAGAGAACTCACCAAGTTCTTCACTGACTTGCGTACAGACGTGAACCACCCAGAACTACCCATCATCTTGGTTTGTCTTCTTCGTGTCTATAAATAAATAGGTAGTATCCTCTTTTGTATTTGGATGCTAATTAAATTTATATGTAAAATATATATATGCAGGTGAAGATAGTAACTCATGATTTTTTCATAAGTCCAACTATGAACTTCAAGGAAGATGTATGGAAGGCTCAAGAGGCAGTGACAGAGAAGCTACCAAACGTGAGAATGGTGGATGGTTTGATAGTTGTGGGCAACTTTGATGAAGGCCTTAATGAAGATAGAGGTCATCTCAATGTTAAATCTGAAGTCAAATTGGGCCAAATGTTTGCTCATGTCTTTTACTCTGACTTTGCCCACAATCTCACTTACTAA

mRNA sequence

ATGGCTGGTCGAGGTGGCGTCTCCAAAGACCCAATCACAGGCAAAAATATATGGGACGGATATATCCCACCAGAGTCCCAGTCCAATGAATCAATCTTTCGATTCAATGCTAATTCAGAATGGGAACAAGCCTCTGAACCACTCCATTGGGATATTGATGTTAAAAAAACCAATGGAATCGGACCTGGGATGGCTTTTGCCAATGAGCTTTTGGCCAAAGCTGGTAAGAGCATTGGTGGCATCGGTCTTGTTCCGTGTGCCATAGGAGGAACTCACTTAAGAGAATGGGTTAAAGGGACTGATAGTTACACCAAATTGGTTGAGCGAATTAAAGCTTCAGAAAAACAAGGGGGAAAAGTCCAAGGATTTTTTTGGTATCAAGGAGAGTCTGATGCTGCAGTAGAAGAAGAATCTAAATGCTATGAAAGAGAACTCACCAAGTTCTTCACTGACTTGCGTACAGACGTGAACCACCCAGAACTACCCATCATCTTGGTGAAGATAGTAACTCATGATTTTTTCATAAGTCCAACTATGAACTTCAAGGAAGATGTATGGAAGGCTCAAGAGGCAGTGACAGAGAAGCTACCAAACGTGAGAATGGTGGATGGTTTGATAGTTGTGGGCAACTTTGATGAAGGCCTTAATGAAGATAGAGGTCATCTCAATGTTAAATCTGAAGTCAAATTGGGCCAAATGTTTGCTCATGTCTTTTACTCTGACTTTGCCCACAATCTCACTTACTAA

Coding sequence (CDS)

ATGGCTGGTCGAGGTGGCGTCTCCAAAGACCCAATCACAGGCAAAAATATATGGGACGGATATATCCCACCAGAGTCCCAGTCCAATGAATCAATCTTTCGATTCAATGCTAATTCAGAATGGGAACAAGCCTCTGAACCACTCCATTGGGATATTGATGTTAAAAAAACCAATGGAATCGGACCTGGGATGGCTTTTGCCAATGAGCTTTTGGCCAAAGCTGGTAAGAGCATTGGTGGCATCGGTCTTGTTCCGTGTGCCATAGGAGGAACTCACTTAAGAGAATGGGTTAAAGGGACTGATAGTTACACCAAATTGGTTGAGCGAATTAAAGCTTCAGAAAAACAAGGGGGAAAAGTCCAAGGATTTTTTTGGTATCAAGGAGAGTCTGATGCTGCAGTAGAAGAAGAATCTAAATGCTATGAAAGAGAACTCACCAAGTTCTTCACTGACTTGCGTACAGACGTGAACCACCCAGAACTACCCATCATCTTGGTGAAGATAGTAACTCATGATTTTTTCATAAGTCCAACTATGAACTTCAAGGAAGATGTATGGAAGGCTCAAGAGGCAGTGACAGAGAAGCTACCAAACGTGAGAATGGTGGATGGTTTGATAGTTGTGGGCAACTTTGATGAAGGCCTTAATGAAGATAGAGGTCATCTCAATGTTAAATCTGAAGTCAAATTGGGCCAAATGTTTGCTCATGTCTTTTACTCTGACTTTGCCCACAATCTCACTTACTAA

Protein sequence

MAGRGGVSKDPITGKNIWDGYIPPESQSNESIFRFNANSEWEQASEPLHWDIDVKKTNGIGPGMAFANELLAKAGKSIGGIGLVPCAIGGTHLREWVKGTDSYTKLVERIKASEKQGGKVQGFFWYQGESDAAVEEESKCYERELTKFFTDLRTDVNHPELPIILVKIVTHDFFISPTMNFKEDVWKAQEAVTEKLPNVRMVDGLIVVGNFDEGLNEDRGHLNVKSEVKLGQMFAHVFYSDFAHNLTY
Homology
BLAST of Spg008434 vs. NCBI nr
Match: KAG7016422.1 (putative carbohydrate esterase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 431.8 bits (1109), Expect = 4.0e-117
Identity = 202/244 (82.79%), Postives = 220/244 (90.16%), Query Frame = 0

Query: 1   MAGRGGVSKDPITGKNIWDGYIPPESQSNESIFRFNANSEWEQASEPLHWDIDVKKTNGI 60
           MAGRGGVSKDP T KN+WDGYIPPESQ N+SIFRF A+  WEQA EPLHWDIDV KTNG+
Sbjct: 18  MAGRGGVSKDPTTDKNVWDGYIPPESQPNQSIFRFTADMVWEQAREPLHWDIDVVKTNGV 77

Query: 61  GPGMAFANELLAKAGKSIGGIGLVPCAIGGTHLREWVKGTDSYTKLVERIKASEKQGGKV 120
           GPGM FANELLAKAG SIG IGLVPCAIGG+HLREWVKGTD YTKLVER+K SE+ GGKV
Sbjct: 78  GPGMPFANELLAKAGPSIGTIGLVPCAIGGSHLREWVKGTDRYTKLVERMKRSEEHGGKV 137

Query: 121 QGFFWYQGESDAAVEEESKCYERELTKFFTDLRTDVNHPELPIILVKIVTHDFFISPTMN 180
           +GFFWYQGESDAAVEEE+K YEREL+KFFTDLR D+NHP+LPIILVKIVTHDFFISP   
Sbjct: 138 KGFFWYQGESDAAVEEEAKSYERELSKFFTDLRADMNHPDLPIILVKIVTHDFFISPDFE 197

Query: 181 FKEDVWKAQEAVTEKLPNVRMVDGLIVVGNFDEGLNEDRGHLNVKSEVKLGQMFAHVFYS 240
           FKE+VW AQEAVT+KLPNVRMVDG + VGNFDEGLNEDRGHLNVKSEV LG+MFAH +YS
Sbjct: 198 FKEEVWNAQEAVTQKLPNVRMVDGRVAVGNFDEGLNEDRGHLNVKSEVNLGKMFAHSYYS 257

Query: 241 DFAH 245
           +FAH
Sbjct: 258 NFAH 261

BLAST of Spg008434 vs. NCBI nr
Match: XP_022993914.1 (probable carbohydrate esterase At4g34215 [Cucurbita maxima])

HSP 1 Score: 431.8 bits (1109), Expect = 4.0e-117
Identity = 202/244 (82.79%), Postives = 220/244 (90.16%), Query Frame = 0

Query: 1   MAGRGGVSKDPITGKNIWDGYIPPESQSNESIFRFNANSEWEQASEPLHWDIDVKKTNGI 60
           MAGRGGVSKDP T KN+WDGYIPPESQ N+SIFRF A+  WEQA EPLHWDIDV KTNG+
Sbjct: 18  MAGRGGVSKDPTTDKNVWDGYIPPESQPNQSIFRFTADMVWEQAREPLHWDIDVVKTNGV 77

Query: 61  GPGMAFANELLAKAGKSIGGIGLVPCAIGGTHLREWVKGTDSYTKLVERIKASEKQGGKV 120
           GPGM FANELLAKAG SIG IGLVPCAIGG+HLREWVKGTD YTKLVER+K SE+ GGKV
Sbjct: 78  GPGMPFANELLAKAGPSIGTIGLVPCAIGGSHLREWVKGTDRYTKLVERMKRSEEHGGKV 137

Query: 121 QGFFWYQGESDAAVEEESKCYERELTKFFTDLRTDVNHPELPIILVKIVTHDFFISPTMN 180
           +GFFWYQGESDAAVEEE+K YEREL+KFFTDLR DVNHP+LPIILVKIVTHDFFISP   
Sbjct: 138 KGFFWYQGESDAAVEEEAKSYERELSKFFTDLRADVNHPDLPIILVKIVTHDFFISPDFE 197

Query: 181 FKEDVWKAQEAVTEKLPNVRMVDGLIVVGNFDEGLNEDRGHLNVKSEVKLGQMFAHVFYS 240
           FK++VW AQEAVT+KLPNVRMVDG + VGNFDEGLNEDRGHLNVKSEV LG+MFAH +YS
Sbjct: 198 FKDEVWNAQEAVTQKLPNVRMVDGRVAVGNFDEGLNEDRGHLNVKSEVNLGKMFAHSYYS 257

Query: 241 DFAH 245
           +FAH
Sbjct: 258 NFAH 261

BLAST of Spg008434 vs. NCBI nr
Match: XP_023550941.1 (probable carbohydrate esterase At4g34215 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 431.8 bits (1109), Expect = 4.0e-117
Identity = 202/244 (82.79%), Postives = 220/244 (90.16%), Query Frame = 0

Query: 1   MAGRGGVSKDPITGKNIWDGYIPPESQSNESIFRFNANSEWEQASEPLHWDIDVKKTNGI 60
           MAGRGGVSKDP T KN+WDGYIPPESQ N+SIFRF A+  WEQA EPLHWDIDV KTNG+
Sbjct: 18  MAGRGGVSKDPTTDKNVWDGYIPPESQPNQSIFRFTADMVWEQAHEPLHWDIDVVKTNGV 77

Query: 61  GPGMAFANELLAKAGKSIGGIGLVPCAIGGTHLREWVKGTDSYTKLVERIKASEKQGGKV 120
           GPGM FANELLAKAG SIG IGLVPCAIGG+HLREWVKGTD YTKLVER+K SE+ GGKV
Sbjct: 78  GPGMPFANELLAKAGPSIGTIGLVPCAIGGSHLREWVKGTDRYTKLVERMKRSEEHGGKV 137

Query: 121 QGFFWYQGESDAAVEEESKCYERELTKFFTDLRTDVNHPELPIILVKIVTHDFFISPTMN 180
           +GFFWYQGESDAAVEEE+K YEREL+KFFTDLR D+NHP+LPIILVKIVTHDFFISP   
Sbjct: 138 KGFFWYQGESDAAVEEEAKSYERELSKFFTDLRADMNHPDLPIILVKIVTHDFFISPDFE 197

Query: 181 FKEDVWKAQEAVTEKLPNVRMVDGLIVVGNFDEGLNEDRGHLNVKSEVKLGQMFAHVFYS 240
           FKE+VW AQEAVT+KLPNVRMVDG + VGNFDEGLNEDRGHLNVKSEV LG+MFAH +YS
Sbjct: 198 FKEEVWNAQEAVTQKLPNVRMVDGRVAVGNFDEGLNEDRGHLNVKSEVNLGKMFAHSYYS 257

Query: 241 DFAH 245
           +FAH
Sbjct: 258 NFAH 261

BLAST of Spg008434 vs. NCBI nr
Match: KAG6578894.1 (putative carbohydrate esterase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 430.6 bits (1106), Expect = 8.9e-117
Identity = 201/244 (82.38%), Postives = 220/244 (90.16%), Query Frame = 0

Query: 1   MAGRGGVSKDPITGKNIWDGYIPPESQSNESIFRFNANSEWEQASEPLHWDIDVKKTNGI 60
           MAGRGGVSKDP T KN+WDGYIPPESQ N+SIFRF A+  WEQA EPLHWDIDV KTNG+
Sbjct: 18  MAGRGGVSKDPTTDKNVWDGYIPPESQPNQSIFRFTADMVWEQAREPLHWDIDVVKTNGV 77

Query: 61  GPGMAFANELLAKAGKSIGGIGLVPCAIGGTHLREWVKGTDSYTKLVERIKASEKQGGKV 120
           GPGM FANELLAKAG SIG IGLVPCAIGG+HLREWVKGTD YTKLVER+K SE+ GGKV
Sbjct: 78  GPGMPFANELLAKAGPSIGTIGLVPCAIGGSHLREWVKGTDRYTKLVERMKRSEEHGGKV 137

Query: 121 QGFFWYQGESDAAVEEESKCYERELTKFFTDLRTDVNHPELPIILVKIVTHDFFISPTMN 180
           +GFFWYQGESDAAVEEE+K YEREL+KFFTDLR D+NHP+LPIILVKIVTHDFFISP   
Sbjct: 138 KGFFWYQGESDAAVEEEAKSYERELSKFFTDLRADMNHPDLPIILVKIVTHDFFISPDFE 197

Query: 181 FKEDVWKAQEAVTEKLPNVRMVDGLIVVGNFDEGLNEDRGHLNVKSEVKLGQMFAHVFYS 240
           FKE+VW AQEAVT+KLPNVRMVDG + VGNFDEGLNEDRGHLNV+SEV LG+MFAH +YS
Sbjct: 198 FKEEVWNAQEAVTQKLPNVRMVDGRVAVGNFDEGLNEDRGHLNVRSEVNLGKMFAHSYYS 257

Query: 241 DFAH 245
           +FAH
Sbjct: 258 NFAH 261

BLAST of Spg008434 vs. NCBI nr
Match: XP_022939276.1 (probable carbohydrate esterase At4g34215 [Cucurbita moschata])

HSP 1 Score: 429.5 bits (1103), Expect = 2.0e-116
Identity = 200/244 (81.97%), Postives = 220/244 (90.16%), Query Frame = 0

Query: 1   MAGRGGVSKDPITGKNIWDGYIPPESQSNESIFRFNANSEWEQASEPLHWDIDVKKTNGI 60
           MAGRGGVSKDP T KN+WDGYIPPESQ N+SIFRF A+  WEQA EPLHWDIDV KTNG+
Sbjct: 18  MAGRGGVSKDPTTDKNVWDGYIPPESQPNQSIFRFTADMVWEQAREPLHWDIDVVKTNGV 77

Query: 61  GPGMAFANELLAKAGKSIGGIGLVPCAIGGTHLREWVKGTDSYTKLVERIKASEKQGGKV 120
           GPGM FANELLAKAG SIG IGLVPCAIGG+HLREWVKGT+ YTKLVER+K SE+ GGKV
Sbjct: 78  GPGMPFANELLAKAGPSIGTIGLVPCAIGGSHLREWVKGTNRYTKLVERMKRSEEHGGKV 137

Query: 121 QGFFWYQGESDAAVEEESKCYERELTKFFTDLRTDVNHPELPIILVKIVTHDFFISPTMN 180
           +GFFWYQGESDAAVEEE+K YEREL+KFFTDLR D+NHP+LPIILVKIVTHDFFISP   
Sbjct: 138 KGFFWYQGESDAAVEEEAKSYERELSKFFTDLRADMNHPDLPIILVKIVTHDFFISPDFE 197

Query: 181 FKEDVWKAQEAVTEKLPNVRMVDGLIVVGNFDEGLNEDRGHLNVKSEVKLGQMFAHVFYS 240
           FKE+VW AQEAVT+KLPN+RMVDG + VGNFDEGLNEDRGHLNVKSEV LG+MFAH +YS
Sbjct: 198 FKEEVWNAQEAVTQKLPNIRMVDGRVAVGNFDEGLNEDRGHLNVKSEVNLGKMFAHSYYS 257

Query: 241 DFAH 245
           +FAH
Sbjct: 258 NFAH 261

BLAST of Spg008434 vs. ExPASy Swiss-Prot
Match: Q8L9J9 (Probable carbohydrate esterase At4g34215 OS=Arabidopsis thaliana OX=3702 GN=At4g34215 PE=1 SV=2)

HSP 1 Score: 175.6 bits (444), Expect = 6.8e-43
Identity = 98/242 (40.50%), Postives = 137/242 (56.61%), Query Frame = 0

Query: 1   MAGRGGVSKDPITGKNIWDGYIPPESQSNESIFRFNANSEWEQASEPLHWDIDVKKTNGI 60
           MAGRGGV KD    + +WD  +PPE   N SI R +A+  WE+A EPLH DID  K  G+
Sbjct: 33  MAGRGGVFKDHHNNRWVWDKILPPECAPNSSILRLSADLRWEEAHEPLHVDIDTGKVCGV 92

Query: 61  GPGMAFANELLAKAGKSIGGIGLVPCAIGGTHLREWVKGTDSYTKLVERIKASEKQGGKV 120
           GPGMAFAN +  +       IGLVPCA GGT ++EW +G+  Y ++V+R + S K GG++
Sbjct: 93  GPGMAFANAVKNRLETDSAVIGLVPCASGGTAIKEWERGSHLYERMVKRTEESRKCGGEI 152

Query: 121 QGFFWYQGESDAAVEEESKCYERELTKFFTDLRTDVNHPELPIILVKIVTHDFFISPTMN 180
           +   WYQGESD     +++ Y   + +   +LR D+N P LPII V I +   +I     
Sbjct: 153 KAVLWYQGESDVLDIHDAESYGNNMDRLIKNLRHDLNLPSLPIIQVAIASGGGYI----- 212

Query: 181 FKEDVWKAQEAVTEKLPNVRMVDGLIVVGNFDEGLNEDRGHLNVKSEVKLGQMFAHVFYS 240
             + V +AQ  +  KL NV  VD   +       L  D  HL  +++V+LG   A  + S
Sbjct: 213 --DKVREAQLGL--KLSNVVCVDAKGL------PLKSDNLHLTTEAQVQLGLSLAQAYLS 259

Query: 241 DF 243
           +F
Sbjct: 273 NF 259

BLAST of Spg008434 vs. ExPASy TrEMBL
Match: A0A6J1K1G7 (probable carbohydrate esterase At4g34215 OS=Cucurbita maxima OX=3661 GN=LOC111489769 PE=4 SV=1)

HSP 1 Score: 431.8 bits (1109), Expect = 1.9e-117
Identity = 202/244 (82.79%), Postives = 220/244 (90.16%), Query Frame = 0

Query: 1   MAGRGGVSKDPITGKNIWDGYIPPESQSNESIFRFNANSEWEQASEPLHWDIDVKKTNGI 60
           MAGRGGVSKDP T KN+WDGYIPPESQ N+SIFRF A+  WEQA EPLHWDIDV KTNG+
Sbjct: 18  MAGRGGVSKDPTTDKNVWDGYIPPESQPNQSIFRFTADMVWEQAREPLHWDIDVVKTNGV 77

Query: 61  GPGMAFANELLAKAGKSIGGIGLVPCAIGGTHLREWVKGTDSYTKLVERIKASEKQGGKV 120
           GPGM FANELLAKAG SIG IGLVPCAIGG+HLREWVKGTD YTKLVER+K SE+ GGKV
Sbjct: 78  GPGMPFANELLAKAGPSIGTIGLVPCAIGGSHLREWVKGTDRYTKLVERMKRSEEHGGKV 137

Query: 121 QGFFWYQGESDAAVEEESKCYERELTKFFTDLRTDVNHPELPIILVKIVTHDFFISPTMN 180
           +GFFWYQGESDAAVEEE+K YEREL+KFFTDLR DVNHP+LPIILVKIVTHDFFISP   
Sbjct: 138 KGFFWYQGESDAAVEEEAKSYERELSKFFTDLRADVNHPDLPIILVKIVTHDFFISPDFE 197

Query: 181 FKEDVWKAQEAVTEKLPNVRMVDGLIVVGNFDEGLNEDRGHLNVKSEVKLGQMFAHVFYS 240
           FK++VW AQEAVT+KLPNVRMVDG + VGNFDEGLNEDRGHLNVKSEV LG+MFAH +YS
Sbjct: 198 FKDEVWNAQEAVTQKLPNVRMVDGRVAVGNFDEGLNEDRGHLNVKSEVNLGKMFAHSYYS 257

Query: 241 DFAH 245
           +FAH
Sbjct: 258 NFAH 261

BLAST of Spg008434 vs. ExPASy TrEMBL
Match: A0A6J1FFF9 (probable carbohydrate esterase At4g34215 OS=Cucurbita moschata OX=3662 GN=LOC111445241 PE=4 SV=1)

HSP 1 Score: 429.5 bits (1103), Expect = 9.6e-117
Identity = 200/244 (81.97%), Postives = 220/244 (90.16%), Query Frame = 0

Query: 1   MAGRGGVSKDPITGKNIWDGYIPPESQSNESIFRFNANSEWEQASEPLHWDIDVKKTNGI 60
           MAGRGGVSKDP T KN+WDGYIPPESQ N+SIFRF A+  WEQA EPLHWDIDV KTNG+
Sbjct: 18  MAGRGGVSKDPTTDKNVWDGYIPPESQPNQSIFRFTADMVWEQAREPLHWDIDVVKTNGV 77

Query: 61  GPGMAFANELLAKAGKSIGGIGLVPCAIGGTHLREWVKGTDSYTKLVERIKASEKQGGKV 120
           GPGM FANELLAKAG SIG IGLVPCAIGG+HLREWVKGT+ YTKLVER+K SE+ GGKV
Sbjct: 78  GPGMPFANELLAKAGPSIGTIGLVPCAIGGSHLREWVKGTNRYTKLVERMKRSEEHGGKV 137

Query: 121 QGFFWYQGESDAAVEEESKCYERELTKFFTDLRTDVNHPELPIILVKIVTHDFFISPTMN 180
           +GFFWYQGESDAAVEEE+K YEREL+KFFTDLR D+NHP+LPIILVKIVTHDFFISP   
Sbjct: 138 KGFFWYQGESDAAVEEEAKSYERELSKFFTDLRADMNHPDLPIILVKIVTHDFFISPDFE 197

Query: 181 FKEDVWKAQEAVTEKLPNVRMVDGLIVVGNFDEGLNEDRGHLNVKSEVKLGQMFAHVFYS 240
           FKE+VW AQEAVT+KLPN+RMVDG + VGNFDEGLNEDRGHLNVKSEV LG+MFAH +YS
Sbjct: 198 FKEEVWNAQEAVTQKLPNIRMVDGRVAVGNFDEGLNEDRGHLNVKSEVNLGKMFAHSYYS 257

Query: 241 DFAH 245
           +FAH
Sbjct: 258 NFAH 261

BLAST of Spg008434 vs. ExPASy TrEMBL
Match: A0A6J1CJZ1 (probable carbohydrate esterase At4g34215 OS=Momordica charantia OX=3673 GN=LOC111011984 PE=4 SV=1)

HSP 1 Score: 422.5 bits (1085), Expect = 1.2e-114
Identity = 203/247 (82.19%), Postives = 216/247 (87.45%), Query Frame = 0

Query: 1   MAGRGGVSKDPITGKNIWDGYIPPESQSNESIFRFNANSEWEQASEPLHWDIDVKKTNGI 60
           MAGRGGVSKDPIT KNIWDGYIPPESQSNESI R  A+  WEQASEPLHWDID  KTNGI
Sbjct: 25  MAGRGGVSKDPITEKNIWDGYIPPESQSNESILRLTADLRWEQASEPLHWDIDYHKTNGI 84

Query: 61  GPGMAFANELLAKAGKSIGGIGLVPCAIGGTHLREWVKGTDSYTKLVERIKASEKQGGKV 120
           GPGMAFANEL  + GKSIG IGLVPCAIGGTHLREW+KGT  YTKL++RIKASEK GGKV
Sbjct: 85  GPGMAFANELSVQVGKSIGVIGLVPCAIGGTHLREWIKGTAYYTKLIDRIKASEKHGGKV 144

Query: 121 QGFFWYQGESDAAVEEESKCYERELTKFFTDLRTDVNHPELPIILVKIVTHDFFISPTMN 180
           QGF WYQGESDA+VEEESK YE ELTKFFTDLRTD N+ ELPIILVKIVTHD F SP +N
Sbjct: 145 QGFLWYQGESDASVEEESKSYETELTKFFTDLRTDSNNLELPIILVKIVTHDIFTSPIIN 204

Query: 181 FKEDVWKAQEAVTEKLPNVRMVDGLIVVGNFDEGLNEDRGHLNVKSEVKLGQMFAHVFYS 240
           FKEDVWKAQE VTEKL NVRMVDG   VGNF+EGLNED+GHLNVKSEVKLG+M AH FYS
Sbjct: 205 FKEDVWKAQETVTEKLSNVRMVDGSEAVGNFEEGLNEDKGHLNVKSEVKLGKMLAHAFYS 264

Query: 241 DFAHNLT 248
           +F+H LT
Sbjct: 265 NFSHRLT 271

BLAST of Spg008434 vs. ExPASy TrEMBL
Match: A0A5D3CGI9 (Putative carbohydrate esterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold874G00750 PE=4 SV=1)

HSP 1 Score: 404.1 bits (1037), Expect = 4.3e-109
Identity = 190/248 (76.61%), Postives = 212/248 (85.48%), Query Frame = 0

Query: 1   MAGRGGVSKDPITGKNIWDGYIPPESQSNESIFRFNANSEWEQASEPLHWDIDVKKTNGI 60
           MAGRGGVS+DP T K +WDGYIP E QSN+SIFR NA+  WE+A EPLHWDIDV KTNGI
Sbjct: 26  MAGRGGVSQDPTTEKMVWDGYIPLECQSNDSIFRLNADMVWEKAHEPLHWDIDVVKTNGI 85

Query: 61  GPGMAFANELLAKAGKSIGGIGLVPCAIGGTHLREWVKGTDSYTKLVERIKASEKQGGKV 120
           GPGM FANELLA  GK  G IGLVPCAIGG+HL+EWVKGTD Y  LVERI+ASEK GGKV
Sbjct: 86  GPGMPFANELLAIGGKRAGAIGLVPCAIGGSHLKEWVKGTDRYDNLVERIRASEKDGGKV 145

Query: 121 QGFFWYQGESDAAVEEESKCYERELTKFFTDLRTDVNHPELPIILVKIVTHDFFISPTMN 180
           +GF WYQGESDAAVEEE+ CYERELTKFF DLR D NHP+LPII+VKIVTHDFF+SP ++
Sbjct: 146 KGFLWYQGESDAAVEEEAMCYERELTKFFIDLRADTNHPDLPIIMVKIVTHDFFLSPNIS 205

Query: 181 FKEDVWKAQEAVTEKLPNVRMVDGLIVVGNFDEGLNEDRGHLNVKSEVKLGQMFAHVFYS 240
           FKE+V KA EAVT  LPNV MVDG + VGNFD+GLNED+GHLNVKSEVKLG+MFAH FYS
Sbjct: 206 FKEEVLKALEAVTHNLPNVTMVDGPMAVGNFDDGLNEDKGHLNVKSEVKLGKMFAHSFYS 265

Query: 241 DFAHNLTY 249
           +FAHN  +
Sbjct: 266 NFAHNFLF 273

BLAST of Spg008434 vs. ExPASy TrEMBL
Match: A0A1S3B3Y0 (probable carbohydrate esterase At4g34215 OS=Cucumis melo OX=3656 GN=LOC103485730 PE=4 SV=1)

HSP 1 Score: 404.1 bits (1037), Expect = 4.3e-109
Identity = 190/248 (76.61%), Postives = 212/248 (85.48%), Query Frame = 0

Query: 1   MAGRGGVSKDPITGKNIWDGYIPPESQSNESIFRFNANSEWEQASEPLHWDIDVKKTNGI 60
           MAGRGGVS+DP T K +WDGYIP E QSN+SIFR NA+  WE+A EPLHWDIDV KTNGI
Sbjct: 26  MAGRGGVSQDPTTEKMVWDGYIPLECQSNDSIFRLNADMVWEKAHEPLHWDIDVVKTNGI 85

Query: 61  GPGMAFANELLAKAGKSIGGIGLVPCAIGGTHLREWVKGTDSYTKLVERIKASEKQGGKV 120
           GPGM FANELLA  GK  G IGLVPCAIGG+HL+EWVKGTD Y  LVERI+ASEK GGKV
Sbjct: 86  GPGMPFANELLAIGGKRAGAIGLVPCAIGGSHLKEWVKGTDRYDNLVERIRASEKDGGKV 145

Query: 121 QGFFWYQGESDAAVEEESKCYERELTKFFTDLRTDVNHPELPIILVKIVTHDFFISPTMN 180
           +GF WYQGESDAAVEEE+ CYERELTKFF DLR D NHP+LPII+VKIVTHDFF+SP ++
Sbjct: 146 KGFLWYQGESDAAVEEEAMCYERELTKFFIDLRADTNHPDLPIIMVKIVTHDFFLSPNIS 205

Query: 181 FKEDVWKAQEAVTEKLPNVRMVDGLIVVGNFDEGLNEDRGHLNVKSEVKLGQMFAHVFYS 240
           FKE+V KA EAVT  LPNV MVDG + VGNFD+GLNED+GHLNVKSEVKLG+MFAH FYS
Sbjct: 206 FKEEVLKALEAVTHNLPNVTMVDGPMAVGNFDDGLNEDKGHLNVKSEVKLGKMFAHSFYS 265

Query: 241 DFAHNLTY 249
           +FAHN  +
Sbjct: 266 NFAHNFLF 273

BLAST of Spg008434 vs. TAIR 10
Match: AT3G53010.1 (Domain of unknown function (DUF303) )

HSP 1 Score: 192.2 bits (487), Expect = 5.0e-49
Identity = 107/240 (44.58%), Postives = 138/240 (57.50%), Query Frame = 0

Query: 1   MAGRGGVSKDPITGKNIWDGYIPPESQSNESIFRFNANSEWEQASEPLHWDIDVKKTNGI 60
           MAGRGGV  D  T   +WDG IPPE +SN SI R  +  EW++A EPLH DID+ KTNG+
Sbjct: 40  MAGRGGVYNDTATNTTVWDGVIPPECRSNPSILRLTSKLEWKEAKEPLHVDIDINKTNGV 99

Query: 61  GPGMAFANELLAKAGKSIGGIGLVPCAIGGTHLREWVKGTDSYTKLVERIKASEKQ--GG 120
           GPGM FAN ++ + G+    +GLVPC+IGGT L +W KG   Y + V+R KA+     GG
Sbjct: 100 GPGMPFANRVVNRFGQ----VGLVPCSIGGTKLSQWQKGEFLYEETVKRAKAAMASGGGG 159

Query: 121 KVQGFFWYQGESDAAVEEESKCYERELTKFFTDLRTDVNHPELPIILVKIVTHDFFISPT 180
             +   WYQGESD     ++  Y++ L KFF+DLR D+ HP LPII V + T        
Sbjct: 160 SYRAVLWYQGESDTVDMVDASVYKKRLVKFFSDLRNDLQHPNLPIIQVALAT------GA 219

Query: 181 MNFKEDVWKAQEAVTEKLPNVRMVDGLIVVGNFDEGLNEDRGHLNVKSEVKLGQMFAHVF 239
             + + V KAQ  +   L NV  VD   +       L  D  HL   S+V+LG M A  F
Sbjct: 220 GPYLDAVRKAQ--LKTDLENVYCVDARGL------PLEPDGLHLTTSSQVQLGHMIAESF 261

BLAST of Spg008434 vs. TAIR 10
Match: AT4G34215.1 (Domain of unknown function (DUF303) )

HSP 1 Score: 175.6 bits (444), Expect = 4.8e-44
Identity = 98/242 (40.50%), Postives = 137/242 (56.61%), Query Frame = 0

Query: 1   MAGRGGVSKDPITGKNIWDGYIPPESQSNESIFRFNANSEWEQASEPLHWDIDVKKTNGI 60
           MAGRGGV KD    + +WD  +PPE   N SI R +A+  WE+A EPLH DID  K  G+
Sbjct: 33  MAGRGGVFKDHHNNRWVWDKILPPECAPNSSILRLSADLRWEEAHEPLHVDIDTGKVCGV 92

Query: 61  GPGMAFANELLAKAGKSIGGIGLVPCAIGGTHLREWVKGTDSYTKLVERIKASEKQGGKV 120
           GPGMAFAN +  +       IGLVPCA GGT ++EW +G+  Y ++V+R + S K GG++
Sbjct: 93  GPGMAFANAVKNRLETDSAVIGLVPCASGGTAIKEWERGSHLYERMVKRTEESRKCGGEI 152

Query: 121 QGFFWYQGESDAAVEEESKCYERELTKFFTDLRTDVNHPELPIILVKIVTHDFFISPTMN 180
           +   WYQGESD     +++ Y   + +   +LR D+N P LPII V I +   +I     
Sbjct: 153 KAVLWYQGESDVLDIHDAESYGNNMDRLIKNLRHDLNLPSLPIIQVAIASGGGYI----- 212

Query: 181 FKEDVWKAQEAVTEKLPNVRMVDGLIVVGNFDEGLNEDRGHLNVKSEVKLGQMFAHVFYS 240
             + V +AQ  +  KL NV  VD   +       L  D  HL  +++V+LG   A  + S
Sbjct: 213 --DKVREAQLGL--KLSNVVCVDAKGL------PLKSDNLHLTTEAQVQLGLSLAQAYLS 259

Query: 241 DF 243
           +F
Sbjct: 273 NF 259

BLAST of Spg008434 vs. TAIR 10
Match: AT4G34215.2 (Domain of unknown function (DUF303) )

HSP 1 Score: 175.6 bits (444), Expect = 4.8e-44
Identity = 98/242 (40.50%), Postives = 137/242 (56.61%), Query Frame = 0

Query: 1   MAGRGGVSKDPITGKNIWDGYIPPESQSNESIFRFNANSEWEQASEPLHWDIDVKKTNGI 60
           MAGRGGV KD    + +WD  +PPE   N SI R +A+  WE+A EPLH DID  K  G+
Sbjct: 33  MAGRGGVFKDHHNNRWVWDKILPPECAPNSSILRLSADLRWEEAHEPLHVDIDTGKVCGV 92

Query: 61  GPGMAFANELLAKAGKSIGGIGLVPCAIGGTHLREWVKGTDSYTKLVERIKASEKQGGKV 120
           GPGMAFAN +  +       IGLVPCA GGT ++EW +G+  Y ++V+R + S K GG++
Sbjct: 93  GPGMAFANAVKNRLETDSAVIGLVPCASGGTAIKEWERGSHLYERMVKRTEESRKCGGEI 152

Query: 121 QGFFWYQGESDAAVEEESKCYERELTKFFTDLRTDVNHPELPIILVKIVTHDFFISPTMN 180
           +   WYQGESD     +++ Y   + +   +LR D+N P LPII V I +   +I     
Sbjct: 153 KAVLWYQGESDVLDIHDAESYGNNMDRLIKNLRHDLNLPSLPIIQVAIASGGGYI----- 212

Query: 181 FKEDVWKAQEAVTEKLPNVRMVDGLIVVGNFDEGLNEDRGHLNVKSEVKLGQMFAHVFYS 240
             + V +AQ  +  KL NV  VD   +       L  D  HL  +++V+LG   A  + S
Sbjct: 213 --DKVREAQLGL--KLSNVVCVDAKGL------PLKSDNLHLTTEAQVQLGLSLAQAYLS 259

Query: 241 DF 243
           +F
Sbjct: 273 NF 259

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7016422.14.0e-11782.79putative carbohydrate esterase, partial [Cucurbita argyrosperma subsp. argyrospe... [more]
XP_022993914.14.0e-11782.79probable carbohydrate esterase At4g34215 [Cucurbita maxima][more]
XP_023550941.14.0e-11782.79probable carbohydrate esterase At4g34215 [Cucurbita pepo subsp. pepo][more]
KAG6578894.18.9e-11782.38putative carbohydrate esterase, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022939276.12.0e-11681.97probable carbohydrate esterase At4g34215 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q8L9J96.8e-4340.50Probable carbohydrate esterase At4g34215 OS=Arabidopsis thaliana OX=3702 GN=At4g... [more]
Match NameE-valueIdentityDescription
A0A6J1K1G71.9e-11782.79probable carbohydrate esterase At4g34215 OS=Cucurbita maxima OX=3661 GN=LOC11148... [more]
A0A6J1FFF99.6e-11781.97probable carbohydrate esterase At4g34215 OS=Cucurbita moschata OX=3662 GN=LOC111... [more]
A0A6J1CJZ11.2e-11482.19probable carbohydrate esterase At4g34215 OS=Momordica charantia OX=3673 GN=LOC11... [more]
A0A5D3CGI94.3e-10976.61Putative carbohydrate esterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s... [more]
A0A1S3B3Y04.3e-10976.61probable carbohydrate esterase At4g34215 OS=Cucumis melo OX=3656 GN=LOC103485730... [more]
Match NameE-valueIdentityDescription
AT3G53010.15.0e-4944.58Domain of unknown function (DUF303) [more]
AT4G34215.14.8e-4440.50Domain of unknown function (DUF303) [more]
AT4G34215.24.8e-4440.50Domain of unknown function (DUF303) [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036514SGNH hydrolase superfamilyGENE3D3.40.50.1110SGNH hydrolasecoord: 1..242
e-value: 2.0E-53
score: 183.7
IPR005181Sialate O-acetylesterase domainPFAMPF03629SASAcoord: 1..239
e-value: 1.1E-65
score: 221.4
NoneNo IPR availablePANTHERPTHR31988ESTERASE, PUTATIVE (DUF303)-RELATEDcoord: 1..242
NoneNo IPR availablePANTHERPTHR31988:SF19BNACNNG62850D PROTEINcoord: 1..242
NoneNo IPR availableSUPERFAMILY52266SGNH hydrolasecoord: 23..240

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg008434.1Spg008434.1mRNA