Spg008422 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg008422
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionAcyl-[acyl-carrier-protein] desaturase
Locationscaffold10: 36029923 .. 36030525 (+)
RNA-Seq ExpressionSpg008422
SyntenySpg008422
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACACGTAAATACGACCCACGTGGACAGGATCCAGGAACGGAGAACAACCCATACTTGGGTTTCGTGTACACGTCATTTCAGGAGCGAGCCACGTTCGTATCCCACGGCAACACGGCCCGTTTAGCCAAAGACGGCGGCGACCCAGTCCTCGCCCGCATCTGCGGCACCATCGCCTCCGACGAGAAGCGCCACGAGAACGCCTACTGCAGAATCGTCCAGAAGCTTCTCGACGTCGACCCCACAGGCGCCATGCTGGCAATCGCCGACATGATGCACAAGAAGATCACAATGCCGGCTCACCTAATGTACGATGGGCGCGACCCCAAGCTATTCGACCACTTCTCCGCCGTGGCTCAGCGGCTCGGGATCTACACGGCTAACGACTACGCCGATATATTGGAGGTGCTGATCGAACGGTGGAGGCTGGAGAAGGTGGAGGGATTGACGGGCGAGGGAAGACGGGCTCAGGACTTCGTGTGCGGCTTGGCGCCGAGGATTAGGAAGCTTCAGGAGCGAGCCGATGAGCGAGCTAAGAAGTTGAAGCCGCATGGGGTTAAGTTTAGCTGGATATTTAATAAGGAAATCCCCATTGTCTCGTAG

mRNA sequence

ATGACACGTAAATACGACCCACGTGGACAGGATCCAGGAACGGAGAACAACCCATACTTGGGTTTCGTGTACACGTCATTTCAGGAGCGAGCCACGTTCGTATCCCACGGCAACACGGCCCGTTTAGCCAAAGACGGCGGCGACCCAGTCCTCGCCCGCATCTGCGGCACCATCGCCTCCGACGAGAAGCGCCACGAGAACGCCTACTGCAGAATCGTCCAGAAGCTTCTCGACGTCGACCCCACAGGCGCCATGCTGGCAATCGCCGACATGATGCACAAGAAGATCACAATGCCGGCTCACCTAATGTACGATGGGCGCGACCCCAAGCTATTCGACCACTTCTCCGCCGTGGCTCAGCGGCTCGGGATCTACACGGCTAACGACTACGCCGATATATTGGAGGTGCTGATCGAACGGTGGAGGCTGGAGAAGGTGGAGGGATTGACGGGCGAGGGAAGACGGGCTCAGGACTTCGTGTGCGGCTTGGCGCCGAGGATTAGGAAGCTTCAGGAGCGAGCCGATGAGCGAGCTAAGAAGTTGAAGCCGCATGGGGTTAAGTTTAGCTGGATATTTAATAAGGAAATCCCCATTGTCTCGTAG

Coding sequence (CDS)

ATGACACGTAAATACGACCCACGTGGACAGGATCCAGGAACGGAGAACAACCCATACTTGGGTTTCGTGTACACGTCATTTCAGGAGCGAGCCACGTTCGTATCCCACGGCAACACGGCCCGTTTAGCCAAAGACGGCGGCGACCCAGTCCTCGCCCGCATCTGCGGCACCATCGCCTCCGACGAGAAGCGCCACGAGAACGCCTACTGCAGAATCGTCCAGAAGCTTCTCGACGTCGACCCCACAGGCGCCATGCTGGCAATCGCCGACATGATGCACAAGAAGATCACAATGCCGGCTCACCTAATGTACGATGGGCGCGACCCCAAGCTATTCGACCACTTCTCCGCCGTGGCTCAGCGGCTCGGGATCTACACGGCTAACGACTACGCCGATATATTGGAGGTGCTGATCGAACGGTGGAGGCTGGAGAAGGTGGAGGGATTGACGGGCGAGGGAAGACGGGCTCAGGACTTCGTGTGCGGCTTGGCGCCGAGGATTAGGAAGCTTCAGGAGCGAGCCGATGAGCGAGCTAAGAAGTTGAAGCCGCATGGGGTTAAGTTTAGCTGGATATTTAATAAGGAAATCCCCATTGTCTCGTAG

Protein sequence

MTRKYDPRGQDPGTENNPYLGFVYTSFQERATFVSHGNTARLAKDGGDPVLARICGTIASDEKRHENAYCRIVQKLLDVDPTGAMLAIADMMHKKITMPAHLMYDGRDPKLFDHFSAVAQRLGIYTANDYADILEVLIERWRLEKVEGLTGEGRRAQDFVCGLAPRIRKLQERADERAKKLKPHGVKFSWIFNKEIPIVS
Homology
BLAST of Spg008422 vs. NCBI nr
Match: XP_038884322.1 (stearoyl-[acyl-carrier-protein] 9-desaturase 6, chloroplastic [Benincasa hispida])

HSP 1 Score: 389.8 bits (1000), Expect = 1.4e-104
Identity = 186/192 (96.88%), Postives = 190/192 (98.96%), Query Frame = 0

Query: 9   GQDPGTENNPYLGFVYTSFQERATFVSHGNTARLAKDGGDPVLARICGTIASDEKRHENA 68
           G DPGTENNPYLGFVYTSFQERATF+SHGNTARLAKDGGDPVLARICGTIASDEKRHENA
Sbjct: 207 GMDPGTENNPYLGFVYTSFQERATFISHGNTARLAKDGGDPVLARICGTIASDEKRHENA 266

Query: 69  YCRIVQKLLDVDPTGAMLAIADMMHKKITMPAHLMYDGRDPKLFDHFSAVAQRLGIYTAN 128
           Y RIVQKLLDVDPTGAMLAIADMMHKKITMPAHLMYDG+DP+LFDHFSAVAQRLGIYTAN
Sbjct: 267 YSRIVQKLLDVDPTGAMLAIADMMHKKITMPAHLMYDGQDPRLFDHFSAVAQRLGIYTAN 326

Query: 129 DYADILEVLIERWRLEKVEGLTGEGRRAQDFVCGLAPRIRKLQERADERAKKLKPHGVKF 188
           DYADILEVLIERWRLEKVEGLTGEGRRAQDFVCGLAPRIRKLQERADERAKKLKPHGVKF
Sbjct: 327 DYADILEVLIERWRLEKVEGLTGEGRRAQDFVCGLAPRIRKLQERADERAKKLKPHGVKF 386

Query: 189 SWIFNKEIPIVS 201
           SWIFNKEIPI+S
Sbjct: 387 SWIFNKEIPILS 398

BLAST of Spg008422 vs. NCBI nr
Match: KAA0058877.1 (stearoyl-(acyl-carrier-protein) 9-desaturase 6 [Cucumis melo var. makuwa] >TYK23746.1 stearoyl-(acyl-carrier-protein) 9-desaturase 6 [Cucumis melo var. makuwa])

HSP 1 Score: 376.7 bits (966), Expect = 1.2e-100
Identity = 181/192 (94.27%), Postives = 186/192 (96.88%), Query Frame = 0

Query: 9   GQDPGTENNPYLGFVYTSFQERATFVSHGNTARLAKDGGDPVLARICGTIASDEKRHENA 68
           G DPGTENNPYLGFVYTSFQERATF+SHGNTARLAK  GDPVLARICGTIASDEKRHENA
Sbjct: 201 GMDPGTENNPYLGFVYTSFQERATFISHGNTARLAKHRGDPVLARICGTIASDEKRHENA 260

Query: 69  YCRIVQKLLDVDPTGAMLAIADMMHKKITMPAHLMYDGRDPKLFDHFSAVAQRLGIYTAN 128
           Y RIVQKLL+VDPTGAMLAIADMM KKITMPAHLMYDG+DP+LFDHFSAVAQRLGIYTAN
Sbjct: 261 YSRIVQKLLEVDPTGAMLAIADMMQKKITMPAHLMYDGQDPRLFDHFSAVAQRLGIYTAN 320

Query: 129 DYADILEVLIERWRLEKVEGLTGEGRRAQDFVCGLAPRIRKLQERADERAKKLKPHGVKF 188
           DYADILEVLIERW LEKVEGLTGEGRRAQDFVCGLAPRIRKLQERADERAKKLKPHGVKF
Sbjct: 321 DYADILEVLIERWGLEKVEGLTGEGRRAQDFVCGLAPRIRKLQERADERAKKLKPHGVKF 380

Query: 189 SWIFNKEIPIVS 201
           SWIFNKEIPI+S
Sbjct: 381 SWIFNKEIPILS 392

BLAST of Spg008422 vs. NCBI nr
Match: XP_008441656.2 (PREDICTED: stearoyl-[acyl-carrier-protein] 9-desaturase 6, chloroplastic, partial [Cucumis melo])

HSP 1 Score: 376.7 bits (966), Expect = 1.2e-100
Identity = 181/192 (94.27%), Postives = 186/192 (96.88%), Query Frame = 0

Query: 9   GQDPGTENNPYLGFVYTSFQERATFVSHGNTARLAKDGGDPVLARICGTIASDEKRHENA 68
           G DPGTENNPYLGFVYTSFQERATF+SHGNTARLAK  GDPVLARICGTIASDEKRHENA
Sbjct: 225 GMDPGTENNPYLGFVYTSFQERATFISHGNTARLAKHRGDPVLARICGTIASDEKRHENA 284

Query: 69  YCRIVQKLLDVDPTGAMLAIADMMHKKITMPAHLMYDGRDPKLFDHFSAVAQRLGIYTAN 128
           Y RIVQKLL+VDPTGAMLAIADMM KKITMPAHLMYDG+DP+LFDHFSAVAQRLGIYTAN
Sbjct: 285 YSRIVQKLLEVDPTGAMLAIADMMQKKITMPAHLMYDGQDPRLFDHFSAVAQRLGIYTAN 344

Query: 129 DYADILEVLIERWRLEKVEGLTGEGRRAQDFVCGLAPRIRKLQERADERAKKLKPHGVKF 188
           DYADILEVLIERW LEKVEGLTGEGRRAQDFVCGLAPRIRKLQERADERAKKLKPHGVKF
Sbjct: 345 DYADILEVLIERWGLEKVEGLTGEGRRAQDFVCGLAPRIRKLQERADERAKKLKPHGVKF 404

Query: 189 SWIFNKEIPIVS 201
           SWIFNKEIPI+S
Sbjct: 405 SWIFNKEIPILS 416

BLAST of Spg008422 vs. NCBI nr
Match: XP_004148817.1 (stearoyl-[acyl-carrier-protein] 9-desaturase 6, chloroplastic [Cucumis sativus] >KGN47485.1 hypothetical protein Csa_019010 [Cucumis sativus])

HSP 1 Score: 370.5 bits (950), Expect = 8.8e-99
Identity = 179/193 (92.75%), Postives = 186/193 (96.37%), Query Frame = 0

Query: 9   GQDPGTENNPYLGFVYTSFQERATFVSHGNTARLAKDGGDPVLARICGTIASDEKRHENA 68
           G DPGTENNPYLGFVYTSFQERATF+SHGNTARLAK  GDPVLARICGTIASDEKRHENA
Sbjct: 196 GMDPGTENNPYLGFVYTSFQERATFISHGNTARLAKHNGDPVLARICGTIASDEKRHENA 255

Query: 69  YCRIVQKLLDVDPTGAMLAIADMMHKKITMPAHLMYDGRDPKLFDHFSAVAQRLGIYTAN 128
           Y RIVQKLL+VDPTGAMLAIADMM KKITMPAHLMYDG+DP+LFDHFSAVAQRLGIYTAN
Sbjct: 256 YSRIVQKLLEVDPTGAMLAIADMMQKKITMPAHLMYDGQDPRLFDHFSAVAQRLGIYTAN 315

Query: 129 DYADILEVLIERWRLEKVE-GLTGEGRRAQDFVCGLAPRIRKLQERADERAKKLKPHGVK 188
           DYADILEVLI+RW LEKVE GLTGEGRRAQD+VCGLAPRIRKLQERADERAKKLKPHGVK
Sbjct: 316 DYADILEVLIQRWGLEKVEDGLTGEGRRAQDYVCGLAPRIRKLQERADERAKKLKPHGVK 375

Query: 189 FSWIFNKEIPIVS 201
           FSWIFNKEIPI+S
Sbjct: 376 FSWIFNKEIPILS 388

BLAST of Spg008422 vs. NCBI nr
Match: XP_028757626.1 (stearoyl-[acyl-carrier-protein] 9-desaturase 6, chloroplastic-like [Prosopis alba])

HSP 1 Score: 366.7 bits (940), Expect = 1.3e-97
Identity = 170/190 (89.47%), Postives = 184/190 (96.84%), Query Frame = 0

Query: 9   GQDPGTENNPYLGFVYTSFQERATFVSHGNTARLAKDGGDPVLARICGTIASDEKRHENA 68
           G DPGTENNPYLGFVYTSFQERATFVSHGNTARLAK+GGDPVLARICGTIA+DEKRHENA
Sbjct: 200 GMDPGTENNPYLGFVYTSFQERATFVSHGNTARLAKEGGDPVLARICGTIAADEKRHENA 259

Query: 69  YCRIVQKLLDVDPTGAMLAIADMMHKKITMPAHLMYDGRDPKLFDHFSAVAQRLGIYTAN 128
           Y RIV+KLL+VDPTGAMLAIADMMHKKITMPAHLMYDG DP+LF+HFSAVAQR+G+YTAN
Sbjct: 260 YARIVEKLLEVDPTGAMLAIADMMHKKITMPAHLMYDGEDPRLFEHFSAVAQRMGVYTAN 319

Query: 129 DYADILEVLIERWRLEKVEGLTGEGRRAQDFVCGLAPRIRKLQERADERAKKLKPHGVKF 188
           DYADILE LIERWRLEK+EGLTGEGRRA+DFVCGLAPRIRKLQERADERA+K+ PHGVKF
Sbjct: 320 DYADILEFLIERWRLEKLEGLTGEGRRARDFVCGLAPRIRKLQERADERARKMGPHGVKF 379

Query: 189 SWIFNKEIPI 199
           SWIFNKE+ +
Sbjct: 380 SWIFNKEVAL 389

BLAST of Spg008422 vs. ExPASy Swiss-Prot
Match: Q84VY3 (Stearoyl-[acyl-carrier-protein] 9-desaturase 6, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=S-ACP-DES6 PE=2 SV=2)

HSP 1 Score: 326.2 bits (835), Expect = 2.5e-88
Identity = 156/191 (81.68%), Postives = 173/191 (90.58%), Query Frame = 0

Query: 9   GQDPGTENNPYLGFVYTSFQERATFVSHGNTARLAKDGGDPVLARICGTIASDEKRHENA 68
           G DPGTENNPYLGFVYTSFQERATFVSHGNTARLAK  GDPVLARICGTIA+DEKRHENA
Sbjct: 201 GMDPGTENNPYLGFVYTSFQERATFVSHGNTARLAKSAGDPVLARICGTIAADEKRHENA 260

Query: 69  YCRIVQKLLDVDPTGAMLAIADMMHKKITMPAHLMYDGRDPKLFDHFSAVAQRLGIYTAN 128
           Y RIV+KLL++DP GA+ A+ADMM KKITMPAHLM DGRDP LF+HFSAVAQRL +YTA+
Sbjct: 261 YVRIVEKLLEIDPNGAVSAVADMMRKKITMPAHLMTDGRDPMLFEHFSAVAQRLEVYTAD 320

Query: 129 DYADILEVLIERWRLEKVEGLTGEGRRAQDFVCGLAPRIRKLQERADERAKKL-KPHGVK 188
           DYADILE L+ RWRLEK+EGLTGEG+RAQ+FVCGLA RIR+LQERADERAKKL K H V 
Sbjct: 321 DYADILEFLVGRWRLEKLEGLTGEGQRAQEFVCGLAQRIRRLQERADERAKKLKKTHEVC 380

Query: 189 FSWIFNKEIPI 199
           FSWIF+K+I +
Sbjct: 381 FSWIFDKQISV 391

BLAST of Spg008422 vs. ExPASy Swiss-Prot
Match: B8A7A3 (Stearoyl-[acyl-carrier-protein] 9-desaturase 1, chloroplastic OS=Oryza sativa subsp. indica OX=39946 GN=OsI_04666 PE=3 SV=1)

HSP 1 Score: 294.7 bits (753), Expect = 8.1e-79
Identity = 140/189 (74.07%), Postives = 165/189 (87.30%), Query Frame = 0

Query: 9   GQDPGTENNPYLGFVYTSFQERATFVSHGNTARLAKDGGDPVLARICGTIASDEKRHENA 68
           G DPGTENNPYLGFVYTSFQERAT VSHGNTARLA+  GD VLAR CGTIA+DEKRHE A
Sbjct: 191 GMDPGTENNPYLGFVYTSFQERATAVSHGNTARLARAHGDDVLARTCGTIAADEKRHETA 250

Query: 69  YCRIVQKLLDVDPTGAMLAIADMMHKKITMPAHLMYDGRDPKLFDHFSAVAQRLGIYTAN 128
           Y RIV++LL +DP GAMLAIADMMHK+ITMPAHLM+DGRD  LFDHF+AVAQRL +YTA 
Sbjct: 251 YGRIVEQLLRLDPDGAMLAIADMMHKRITMPAHLMHDGRDMNLFDHFAAVAQRLNVYTAR 310

Query: 129 DYADILEVLIERWRLEKVE-GLTGEGRRAQDFVCGLAPRIRKLQERADERAKKLKPHGVK 188
           DYADI+E L++RW+LE +E GL+GEGRRA+DFVCGLA R+R+  ERA++RAKK +   VK
Sbjct: 311 DYADIVEFLVKRWKLETLETGLSGEGRRARDFVCGLAKRMRRAAERAEDRAKKDEQRKVK 370

Query: 189 FSWIFNKEI 197
           FSWI+++E+
Sbjct: 371 FSWIYDREV 379

BLAST of Spg008422 vs. ExPASy Swiss-Prot
Match: Q8LJJ9 (Stearoyl-[acyl-carrier-protein] 9-desaturase 1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0880800 PE=2 SV=1)

HSP 1 Score: 294.7 bits (753), Expect = 8.1e-79
Identity = 140/189 (74.07%), Postives = 165/189 (87.30%), Query Frame = 0

Query: 9   GQDPGTENNPYLGFVYTSFQERATFVSHGNTARLAKDGGDPVLARICGTIASDEKRHENA 68
           G DPGTENNPYLGFVYTSFQERAT VSHGNTARLA+  GD VLAR CGTIA+DEKRHE A
Sbjct: 191 GMDPGTENNPYLGFVYTSFQERATAVSHGNTARLARAHGDDVLARTCGTIAADEKRHETA 250

Query: 69  YCRIVQKLLDVDPTGAMLAIADMMHKKITMPAHLMYDGRDPKLFDHFSAVAQRLGIYTAN 128
           Y RIV++LL +DP GAMLAIADMMHK+ITMPAHLM+DGRD  LFDHF+AVAQRL +YTA 
Sbjct: 251 YGRIVEQLLRLDPDGAMLAIADMMHKRITMPAHLMHDGRDMNLFDHFAAVAQRLNVYTAR 310

Query: 129 DYADILEVLIERWRLEKVE-GLTGEGRRAQDFVCGLAPRIRKLQERADERAKKLKPHGVK 188
           DYADI+E L++RW+LE +E GL+GEGRRA+DFVCGLA R+R+  ERA++RAKK +   VK
Sbjct: 311 DYADIVEFLVKRWKLETLETGLSGEGRRARDFVCGLAKRMRRAAERAEDRAKKDEQRKVK 370

Query: 189 FSWIFNKEI 197
           FSWI+++E+
Sbjct: 371 FSWIYDREV 379

BLAST of Spg008422 vs. ExPASy Swiss-Prot
Match: Q41319 (Stearoyl-[acyl-carrier-protein] 9-desaturase, chloroplastic OS=Solanum commersonii OX=4109 PE=2 SV=2)

HSP 1 Score: 288.5 bits (737), Expect = 5.8e-77
Identity = 133/190 (70.00%), Postives = 159/190 (83.68%), Query Frame = 0

Query: 9   GQDPGTENNPYLGFVYTSFQERATFVSHGNTARLAKDGGDPVLARICGTIASDEKRHENA 68
           G DP TENNPYLGF+YTSFQERATF+SHGNTAR AK+ GD  LA++CG IA+DEKRHE A
Sbjct: 206 GMDPRTENNPYLGFIYTSFQERATFISHGNTARHAKEHGDMKLAQVCGIIAADEKRHETA 265

Query: 69  YCRIVQKLLDVDPTGAMLAIADMMHKKITMPAHLMYDGRDPKLFDHFSAVAQRLGIYTAN 128
           Y +IV+KL +VDP G +LA+ADMM KKI+MPAHLMYDGRD  LF+HFS VAQRLG+YTA 
Sbjct: 266 YTKIVEKLFEVDPDGTVLAVADMMRKKISMPAHLMYDGRDDNLFEHFSTVAQRLGVYTAK 325

Query: 129 DYADILEVLIERWRLEKVEGLTGEGRRAQDFVCGLAPRIRKLQERADERAKKLKPHGVKF 188
           DYADILE L+ RW +EK+ GL+GEG +A+D+VCGLAPRIRKL+ERA  RAK+  P  V F
Sbjct: 326 DYADILEFLVGRWEIEKLTGLSGEGHKARDYVCGLAPRIRKLEERAQARAKQKAP--VPF 385

Query: 189 SWIFNKEIPI 199
           SW+F K+I +
Sbjct: 386 SWVFGKDIKL 393

BLAST of Spg008422 vs. ExPASy Swiss-Prot
Match: P46253 (Stearoyl-[acyl-carrier-protein] 9-desaturase, chloroplastic OS=Solanum tuberosum OX=4113 PE=2 SV=1)

HSP 1 Score: 285.0 bits (728), Expect = 6.4e-76
Identity = 137/190 (72.11%), Postives = 157/190 (82.63%), Query Frame = 0

Query: 9   GQDPGTENNPYLGFVYTSFQERATFVSHGNTARLAKDGGDPVLARICGTIASDEKRHENA 68
           G DP TENNPYLGFVYTS ++  TFVSHGNTARLAK+ GD  LA+ICG+IA+DEKRHE A
Sbjct: 206 GMDPRTENNPYLGFVYTSLRKGVTFVSHGNTARLAKEHGDMKLAQICGSIAADEKRHETA 265

Query: 69  YCRIVQKLLDVDPTGAMLAIADMMHKKITMPAHLMYDGRDPKLFDHFSAVAQRLGIYTAN 128
           Y +IV+KLL+VDP GA+LAI DMM K I+MPAHLMYDGRD  LF+HFSAVAQRLG+YTA 
Sbjct: 266 YTKIVEKLLEVDPDGAVLAIGDMMRKNISMPAHLMYDGRDDNLFEHFSAVAQRLGVYTAK 325

Query: 129 DYADILEVLIERWRLEKVEGLTGEGRRAQDFVCGLAPRIRKLQERADERAKKLKPHGVKF 188
           DYADILE  + RW +EK+ GL+ EGRRAQD+VCGLAPRIRKL+ERA  RAK  K   V F
Sbjct: 326 DYADILEFHVGRWEVEKLTGLSSEGRRAQDYVCGLAPRIRKLEERAQARAKHAK--SVPF 385

Query: 189 SWIFNKEIPI 199
           SWIF KEI +
Sbjct: 386 SWIFGKEIKL 393

BLAST of Spg008422 vs. ExPASy TrEMBL
Match: A0A5D3DJB1 (Acyl-[acyl-carrier-protein] desaturase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1607G00630 PE=3 SV=1)

HSP 1 Score: 376.7 bits (966), Expect = 6.0e-101
Identity = 181/192 (94.27%), Postives = 186/192 (96.88%), Query Frame = 0

Query: 9   GQDPGTENNPYLGFVYTSFQERATFVSHGNTARLAKDGGDPVLARICGTIASDEKRHENA 68
           G DPGTENNPYLGFVYTSFQERATF+SHGNTARLAK  GDPVLARICGTIASDEKRHENA
Sbjct: 201 GMDPGTENNPYLGFVYTSFQERATFISHGNTARLAKHRGDPVLARICGTIASDEKRHENA 260

Query: 69  YCRIVQKLLDVDPTGAMLAIADMMHKKITMPAHLMYDGRDPKLFDHFSAVAQRLGIYTAN 128
           Y RIVQKLL+VDPTGAMLAIADMM KKITMPAHLMYDG+DP+LFDHFSAVAQRLGIYTAN
Sbjct: 261 YSRIVQKLLEVDPTGAMLAIADMMQKKITMPAHLMYDGQDPRLFDHFSAVAQRLGIYTAN 320

Query: 129 DYADILEVLIERWRLEKVEGLTGEGRRAQDFVCGLAPRIRKLQERADERAKKLKPHGVKF 188
           DYADILEVLIERW LEKVEGLTGEGRRAQDFVCGLAPRIRKLQERADERAKKLKPHGVKF
Sbjct: 321 DYADILEVLIERWGLEKVEGLTGEGRRAQDFVCGLAPRIRKLQERADERAKKLKPHGVKF 380

Query: 189 SWIFNKEIPIVS 201
           SWIFNKEIPI+S
Sbjct: 381 SWIFNKEIPILS 392

BLAST of Spg008422 vs. ExPASy TrEMBL
Match: A0A1S3B3X2 (Acyl-[acyl-carrier-protein] desaturase OS=Cucumis melo OX=3656 GN=LOC103485738 PE=3 SV=1)

HSP 1 Score: 376.7 bits (966), Expect = 6.0e-101
Identity = 181/192 (94.27%), Postives = 186/192 (96.88%), Query Frame = 0

Query: 9   GQDPGTENNPYLGFVYTSFQERATFVSHGNTARLAKDGGDPVLARICGTIASDEKRHENA 68
           G DPGTENNPYLGFVYTSFQERATF+SHGNTARLAK  GDPVLARICGTIASDEKRHENA
Sbjct: 225 GMDPGTENNPYLGFVYTSFQERATFISHGNTARLAKHRGDPVLARICGTIASDEKRHENA 284

Query: 69  YCRIVQKLLDVDPTGAMLAIADMMHKKITMPAHLMYDGRDPKLFDHFSAVAQRLGIYTAN 128
           Y RIVQKLL+VDPTGAMLAIADMM KKITMPAHLMYDG+DP+LFDHFSAVAQRLGIYTAN
Sbjct: 285 YSRIVQKLLEVDPTGAMLAIADMMQKKITMPAHLMYDGQDPRLFDHFSAVAQRLGIYTAN 344

Query: 129 DYADILEVLIERWRLEKVEGLTGEGRRAQDFVCGLAPRIRKLQERADERAKKLKPHGVKF 188
           DYADILEVLIERW LEKVEGLTGEGRRAQDFVCGLAPRIRKLQERADERAKKLKPHGVKF
Sbjct: 345 DYADILEVLIERWGLEKVEGLTGEGRRAQDFVCGLAPRIRKLQERADERAKKLKPHGVKF 404

Query: 189 SWIFNKEIPIVS 201
           SWIFNKEIPI+S
Sbjct: 405 SWIFNKEIPILS 416

BLAST of Spg008422 vs. ExPASy TrEMBL
Match: A0A0A0KCQ9 (Acyl-[acyl-carrier-protein] desaturase OS=Cucumis sativus OX=3659 GN=Csa_6G338660 PE=3 SV=1)

HSP 1 Score: 370.5 bits (950), Expect = 4.3e-99
Identity = 179/193 (92.75%), Postives = 186/193 (96.37%), Query Frame = 0

Query: 9   GQDPGTENNPYLGFVYTSFQERATFVSHGNTARLAKDGGDPVLARICGTIASDEKRHENA 68
           G DPGTENNPYLGFVYTSFQERATF+SHGNTARLAK  GDPVLARICGTIASDEKRHENA
Sbjct: 196 GMDPGTENNPYLGFVYTSFQERATFISHGNTARLAKHNGDPVLARICGTIASDEKRHENA 255

Query: 69  YCRIVQKLLDVDPTGAMLAIADMMHKKITMPAHLMYDGRDPKLFDHFSAVAQRLGIYTAN 128
           Y RIVQKLL+VDPTGAMLAIADMM KKITMPAHLMYDG+DP+LFDHFSAVAQRLGIYTAN
Sbjct: 256 YSRIVQKLLEVDPTGAMLAIADMMQKKITMPAHLMYDGQDPRLFDHFSAVAQRLGIYTAN 315

Query: 129 DYADILEVLIERWRLEKVE-GLTGEGRRAQDFVCGLAPRIRKLQERADERAKKLKPHGVK 188
           DYADILEVLI+RW LEKVE GLTGEGRRAQD+VCGLAPRIRKLQERADERAKKLKPHGVK
Sbjct: 316 DYADILEVLIQRWGLEKVEDGLTGEGRRAQDYVCGLAPRIRKLQERADERAKKLKPHGVK 375

Query: 189 FSWIFNKEIPIVS 201
           FSWIFNKEIPI+S
Sbjct: 376 FSWIFNKEIPILS 388

BLAST of Spg008422 vs. ExPASy TrEMBL
Match: A0A6J1FH53 (Acyl-[acyl-carrier-protein] desaturase OS=Cucurbita moschata OX=3662 GN=LOC111445427 PE=3 SV=1)

HSP 1 Score: 365.5 bits (937), Expect = 1.4e-97
Identity = 174/192 (90.62%), Postives = 182/192 (94.79%), Query Frame = 0

Query: 9   GQDPGTENNPYLGFVYTSFQERATFVSHGNTARLAKDGGDPVLARICGTIASDEKRHENA 68
           G DPGTENNPYLGFVYTSFQERATFVSHGNT RLAKD GD VLARICGTIASDEKRHENA
Sbjct: 205 GMDPGTENNPYLGFVYTSFQERATFVSHGNTGRLAKDNGDTVLARICGTIASDEKRHENA 264

Query: 69  YCRIVQKLLDVDPTGAMLAIADMMHKKITMPAHLMYDGRDPKLFDHFSAVAQRLGIYTAN 128
           Y RIV+KLLDVDP+GAMLAI DMM KKITMPAHLMYDG DP+LFDHFSAVAQRLGIYTAN
Sbjct: 265 YSRIVKKLLDVDPSGAMLAIEDMMRKKITMPAHLMYDGEDPRLFDHFSAVAQRLGIYTAN 324

Query: 129 DYADILEVLIERWRLEKVEGLTGEGRRAQDFVCGLAPRIRKLQERADERAKKLKPHGVKF 188
           DYADILEVL+ERW LEKVEGLT EGR+AQD+VCGLAPRIRKLQERADERAKKLKP+GVKF
Sbjct: 325 DYADILEVLVERWALEKVEGLTAEGRQAQDYVCGLAPRIRKLQERADERAKKLKPYGVKF 384

Query: 189 SWIFNKEIPIVS 201
           SWIFNKEIPI+S
Sbjct: 385 SWIFNKEIPIIS 396

BLAST of Spg008422 vs. ExPASy TrEMBL
Match: A0A6J1K3L6 (Acyl-[acyl-carrier-protein] desaturase OS=Cucurbita maxima OX=3661 GN=LOC111489757 PE=3 SV=1)

HSP 1 Score: 364.0 bits (933), Expect = 4.0e-97
Identity = 173/192 (90.10%), Postives = 181/192 (94.27%), Query Frame = 0

Query: 9   GQDPGTENNPYLGFVYTSFQERATFVSHGNTARLAKDGGDPVLARICGTIASDEKRHENA 68
           G DPGTENNPYLGFVYTSFQERATFVSHGNT RLAKD GD  LARICGTIASDEKRHENA
Sbjct: 205 GMDPGTENNPYLGFVYTSFQERATFVSHGNTGRLAKDNGDTTLARICGTIASDEKRHENA 264

Query: 69  YCRIVQKLLDVDPTGAMLAIADMMHKKITMPAHLMYDGRDPKLFDHFSAVAQRLGIYTAN 128
           Y RIV+KLLDVDP+GAMLAI DMM KKITMPAHLMYDG DP+LFDHFSAVAQRLGIYTAN
Sbjct: 265 YSRIVKKLLDVDPSGAMLAIEDMMRKKITMPAHLMYDGEDPRLFDHFSAVAQRLGIYTAN 324

Query: 129 DYADILEVLIERWRLEKVEGLTGEGRRAQDFVCGLAPRIRKLQERADERAKKLKPHGVKF 188
           DYADILEVL+ERW LEKVEGLT EGR+AQD+VCGLAPRIRKLQERADERAKKLKP+GVKF
Sbjct: 325 DYADILEVLVERWALEKVEGLTAEGRQAQDYVCGLAPRIRKLQERADERAKKLKPYGVKF 384

Query: 189 SWIFNKEIPIVS 201
           SWIFNKEIPI+S
Sbjct: 385 SWIFNKEIPIIS 396

BLAST of Spg008422 vs. TAIR 10
Match: AT1G43800.1 (Plant stearoyl-acyl-carrier-protein desaturase family protein )

HSP 1 Score: 326.2 bits (835), Expect = 1.8e-89
Identity = 156/191 (81.68%), Postives = 173/191 (90.58%), Query Frame = 0

Query: 9   GQDPGTENNPYLGFVYTSFQERATFVSHGNTARLAKDGGDPVLARICGTIASDEKRHENA 68
           G DPGTENNPYLGFVYTSFQERATFVSHGNTARLAK  GDPVLARICGTIA+DEKRHENA
Sbjct: 201 GMDPGTENNPYLGFVYTSFQERATFVSHGNTARLAKSAGDPVLARICGTIAADEKRHENA 260

Query: 69  YCRIVQKLLDVDPTGAMLAIADMMHKKITMPAHLMYDGRDPKLFDHFSAVAQRLGIYTAN 128
           Y RIV+KLL++DP GA+ A+ADMM KKITMPAHLM DGRDP LF+HFSAVAQRL +YTA+
Sbjct: 261 YVRIVEKLLEIDPNGAVSAVADMMRKKITMPAHLMTDGRDPMLFEHFSAVAQRLEVYTAD 320

Query: 129 DYADILEVLIERWRLEKVEGLTGEGRRAQDFVCGLAPRIRKLQERADERAKKL-KPHGVK 188
           DYADILE L+ RWRLEK+EGLTGEG+RAQ+FVCGLA RIR+LQERADERAKKL K H V 
Sbjct: 321 DYADILEFLVGRWRLEKLEGLTGEGQRAQEFVCGLAQRIRRLQERADERAKKLKKTHEVC 380

Query: 189 FSWIFNKEIPI 199
           FSWIF+K+I +
Sbjct: 381 FSWIFDKQISV 391

BLAST of Spg008422 vs. TAIR 10
Match: AT3G02630.1 (Plant stearoyl-acyl-carrier-protein desaturase family protein )

HSP 1 Score: 278.1 bits (710), Expect = 5.6e-75
Identity = 129/188 (68.62%), Postives = 154/188 (81.91%), Query Frame = 0

Query: 9   GQDPGTENNPYLGFVYTSFQERATFVSHGNTARLAKDGGDPVLARICGTIASDEKRHENA 68
           G DP TENNPYLGF+YTSFQERATF+SHGNTARLAKD GD  LA+ICGTIA+DE+RHE A
Sbjct: 208 GMDPKTENNPYLGFIYTSFQERATFISHGNTARLAKDRGDLKLAQICGTIAADERRHETA 267

Query: 69  YCRIVQKLLDVDPTGAMLAIADMMHKKITMPAHLMYDGRDPKLFDHFSAVAQRLGIYTAN 128
           Y +IV+KL ++DP G +L +ADMM KKI+MPAHLMYDG+D  LF+HFS VAQRLG+YTA 
Sbjct: 268 YTKIVEKLFEIDPDGTILGLADMMKKKISMPAHLMYDGQDDNLFEHFSTVAQRLGVYTAK 327

Query: 129 DYADILEVLIERWRLEKVEGLTGEGRRAQDFVCGLAPRIRKLQERADERAKKLKPHGVKF 188
           DYADILE L+ERW +E +  L+ EG RAQDFVCGL  RIRK++ERA  RAK+   + + F
Sbjct: 328 DYADILEFLVERWNVETLTDLSSEGHRAQDFVCGLPARIRKIEERAQGRAKEAAKN-IPF 387

Query: 189 SWIFNKEI 197
           SWIF + I
Sbjct: 388 SWIFGRNI 394

BLAST of Spg008422 vs. TAIR 10
Match: AT2G43710.2 (Plant stearoyl-acyl-carrier-protein desaturase family protein )

HSP 1 Score: 276.2 bits (705), Expect = 2.1e-74
Identity = 126/190 (66.32%), Postives = 158/190 (83.16%), Query Frame = 0

Query: 9   GQDPGTENNPYLGFVYTSFQERATFVSHGNTARLAKDGGDPVLARICGTIASDEKRHENA 68
           G DP TENNPYLGF+YTSFQERATF+SHGNTAR AK+ GD  LA+ICGTIA+DEKRHE A
Sbjct: 214 GMDPRTENNPYLGFIYTSFQERATFISHGNTARQAKEHGDIKLAQICGTIAADEKRHETA 273

Query: 69  YCRIVQKLLDVDPTGAMLAIADMMHKKITMPAHLMYDGRDPKLFDHFSAVAQRLGIYTAN 128
           Y +IV+KL ++DP G ++A ADMM KKI+MPAHLMYDGR+  LFD+FS+VAQRLG+YTA 
Sbjct: 274 YTKIVEKLFEIDPDGTVMAFADMMRKKISMPAHLMYDGRNDNLFDNFSSVAQRLGVYTAK 333

Query: 129 DYADILEVLIERWRLEKVEGLTGEGRRAQDFVCGLAPRIRKLQERADERAKKLKPHGVKF 188
           DYADILE L+ RW+++ + GL+GEG +AQD++CGLAPRI++L ERA  RAK  K   + F
Sbjct: 334 DYADILEFLVGRWKIQDLTGLSGEGNKAQDYLCGLAPRIKRLDERAQARAK--KGPKIPF 393

Query: 189 SWIFNKEIPI 199
           SWI ++E+ +
Sbjct: 394 SWIHDREVQL 401

BLAST of Spg008422 vs. TAIR 10
Match: AT2G43710.1 (Plant stearoyl-acyl-carrier-protein desaturase family protein )

HSP 1 Score: 276.2 bits (705), Expect = 2.1e-74
Identity = 126/190 (66.32%), Postives = 158/190 (83.16%), Query Frame = 0

Query: 9   GQDPGTENNPYLGFVYTSFQERATFVSHGNTARLAKDGGDPVLARICGTIASDEKRHENA 68
           G DP TENNPYLGF+YTSFQERATF+SHGNTAR AK+ GD  LA+ICGTIA+DEKRHE A
Sbjct: 214 GMDPRTENNPYLGFIYTSFQERATFISHGNTARQAKEHGDIKLAQICGTIAADEKRHETA 273

Query: 69  YCRIVQKLLDVDPTGAMLAIADMMHKKITMPAHLMYDGRDPKLFDHFSAVAQRLGIYTAN 128
           Y +IV+KL ++DP G ++A ADMM KKI+MPAHLMYDGR+  LFD+FS+VAQRLG+YTA 
Sbjct: 274 YTKIVEKLFEIDPDGTVMAFADMMRKKISMPAHLMYDGRNDNLFDNFSSVAQRLGVYTAK 333

Query: 129 DYADILEVLIERWRLEKVEGLTGEGRRAQDFVCGLAPRIRKLQERADERAKKLKPHGVKF 188
           DYADILE L+ RW+++ + GL+GEG +AQD++CGLAPRI++L ERA  RAK  K   + F
Sbjct: 334 DYADILEFLVGRWKIQDLTGLSGEGNKAQDYLCGLAPRIKRLDERAQARAK--KGPKIPF 393

Query: 189 SWIFNKEIPI 199
           SWI ++E+ +
Sbjct: 394 SWIHDREVQL 401

BLAST of Spg008422 vs. TAIR 10
Match: AT5G16240.1 (Plant stearoyl-acyl-carrier-protein desaturase family protein )

HSP 1 Score: 275.4 bits (703), Expect = 3.6e-74
Identity = 126/188 (67.02%), Postives = 154/188 (81.91%), Query Frame = 0

Query: 9   GQDPGTENNPYLGFVYTSFQERATFVSHGNTARLAKDGGDPVLARICGTIASDEKRHENA 68
           G DP TENNPYLGF+YTSFQERATF+SHGNTARLAKD GD  L +ICGTIA+DE+RHE+A
Sbjct: 206 GMDPKTENNPYLGFIYTSFQERATFISHGNTARLAKDLGDLTLGKICGTIAADERRHEHA 265

Query: 69  YCRIVQKLLDVDPTGAMLAIADMMHKKITMPAHLMYDGRDPKLFDHFSAVAQRLGIYTAN 128
           Y +IV+KL ++DP   ++  ADMM KKI+MPAHLMYDGRD  LFDHFS+VAQRLG+YTA 
Sbjct: 266 YTKIVEKLFEIDPDTTVVGFADMMRKKISMPAHLMYDGRDDNLFDHFSSVAQRLGVYTAK 325

Query: 129 DYADILEVLIERWRLEKVEGLTGEGRRAQDFVCGLAPRIRKLQERADERAKKLKPHGVKF 188
           DYADIL+ L+ERW +EK+  L+ EG RAQD++CGL  RIRKL+ERA  R K+   + + F
Sbjct: 326 DYADILQHLVERWNVEKLSDLSSEGNRAQDYLCGLPARIRKLEERAQGRTKEAAKN-IPF 385

Query: 189 SWIFNKEI 197
           SWIF +E+
Sbjct: 386 SWIFGREV 392

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038884322.11.4e-10496.88stearoyl-[acyl-carrier-protein] 9-desaturase 6, chloroplastic [Benincasa hispida... [more]
KAA0058877.11.2e-10094.27stearoyl-(acyl-carrier-protein) 9-desaturase 6 [Cucumis melo var. makuwa] >TYK23... [more]
XP_008441656.21.2e-10094.27PREDICTED: stearoyl-[acyl-carrier-protein] 9-desaturase 6, chloroplastic, partia... [more]
XP_004148817.18.8e-9992.75stearoyl-[acyl-carrier-protein] 9-desaturase 6, chloroplastic [Cucumis sativus] ... [more]
XP_028757626.11.3e-9789.47stearoyl-[acyl-carrier-protein] 9-desaturase 6, chloroplastic-like [Prosopis alb... [more]
Match NameE-valueIdentityDescription
Q84VY32.5e-8881.68Stearoyl-[acyl-carrier-protein] 9-desaturase 6, chloroplastic OS=Arabidopsis tha... [more]
B8A7A38.1e-7974.07Stearoyl-[acyl-carrier-protein] 9-desaturase 1, chloroplastic OS=Oryza sativa su... [more]
Q8LJJ98.1e-7974.07Stearoyl-[acyl-carrier-protein] 9-desaturase 1, chloroplastic OS=Oryza sativa su... [more]
Q413195.8e-7770.00Stearoyl-[acyl-carrier-protein] 9-desaturase, chloroplastic OS=Solanum commerson... [more]
P462536.4e-7672.11Stearoyl-[acyl-carrier-protein] 9-desaturase, chloroplastic OS=Solanum tuberosum... [more]
Match NameE-valueIdentityDescription
A0A5D3DJB16.0e-10194.27Acyl-[acyl-carrier-protein] desaturase OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
A0A1S3B3X26.0e-10194.27Acyl-[acyl-carrier-protein] desaturase OS=Cucumis melo OX=3656 GN=LOC103485738 P... [more]
A0A0A0KCQ94.3e-9992.75Acyl-[acyl-carrier-protein] desaturase OS=Cucumis sativus OX=3659 GN=Csa_6G33866... [more]
A0A6J1FH531.4e-9790.63Acyl-[acyl-carrier-protein] desaturase OS=Cucurbita moschata OX=3662 GN=LOC11144... [more]
A0A6J1K3L64.0e-9790.10Acyl-[acyl-carrier-protein] desaturase OS=Cucurbita maxima OX=3661 GN=LOC1114897... [more]
Match NameE-valueIdentityDescription
AT1G43800.11.8e-8981.68Plant stearoyl-acyl-carrier-protein desaturase family protein [more]
AT3G02630.15.6e-7568.62Plant stearoyl-acyl-carrier-protein desaturase family protein [more]
AT2G43710.22.1e-7466.32Plant stearoyl-acyl-carrier-protein desaturase family protein [more]
AT2G43710.12.1e-7466.32Plant stearoyl-acyl-carrier-protein desaturase family protein [more]
AT5G16240.13.6e-7467.02Plant stearoyl-acyl-carrier-protein desaturase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012348Ribonucleotide reductase-likeGENE3D1.10.620.20Ribonucleotide Reductase, subunit Acoord: 1..198
e-value: 3.4E-90
score: 304.5
IPR005067Fatty acid desaturase, type 2PFAMPF03405FA_desaturase_2coord: 8..192
e-value: 3.1E-82
score: 276.0
IPR005067Fatty acid desaturase, type 2PANTHERPTHR31155ACYL- ACYL-CARRIER-PROTEIN DESATURASE-RELATEDcoord: 9..197
NoneNo IPR availablePIRSRPIRSR000346-1PIRSR000346-1coord: 9..196
e-value: 2.3E-102
score: 340.8
NoneNo IPR availablePANTHERPTHR31155:SF31STEAROYL-[ACYL-CARRIER-PROTEIN] 9-DESATURASE 6, CHLOROPLASTICcoord: 9..197
IPR005803Fatty acid desaturase type 2, conserved sitePROSITEPS00574FATTY_ACID_DESATUR_2coord: 116..135
IPR009078Ferritin-like superfamilySUPERFAMILY47240Ferritin-likecoord: 7..196

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg008422.1Spg008422.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006633 fatty acid biosynthetic process
biological_process GO:0006631 fatty acid metabolic process
cellular_component GO:0009507 chloroplast
molecular_function GO:0045300 acyl-[acyl-carrier-protein] desaturase activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0102786 stearoyl-[acp] desaturase activity
molecular_function GO:0016491 oxidoreductase activity