Spg008341 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg008341
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionRNA-dependent RNA polymerase
Locationscaffold10: 37826212 .. 37831963 (-)
RNA-Seq ExpressionSpg008341
SyntenySpg008341
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAATTTTCCTTTTTCATTTTAAATTTGAACAAGAGAGATAGCAAATAGCTTTTCTTCAAAGTTCAAAGTACATATAAAAAAAAATGTTGGAAATGTTGACAAAATTATATTGAGAAATATGAAAAAATCTCACCATTCTAATTATAATCAATACTTGTGAAATGAAATAAGCTGTCGAGATTTTGATGCACATTACACTAATTAATAATAATTATTGCACATGCAAATATTATGCTTCAAAAATGCAGTTGAAAACAAATGGCTACGATCGAGATTTTCGGATTTCCGGCAAGCGTCGCCGCCGACGATGTGAAGGAATTTCTGGAGAATCACACCGGCGACGGAACGGTGTCGACGGTGAGGATTTCGAAACCCAAAGACGAAAACTCCCGGTTCGCATTCGCGACGGTCCGGTTCACGAGCAAACTGGCGGCAGAGTACGTGGTGGCGCAGGCGGAGGCAGAGGAGCGGCTGTGGTTCGAAAGTTCGTATCTGAAAGCCAGAGGAGTGGAGAGGGAGATGAGGGCGGCGGCGGCGGAGGCAAGGAGGCGGGGCGAGGTGGAGAGAATCGAAAACGTGAGGATTCACTTGGGAAATCGGATTTCGAAGGAGAGAATGAGTGTGATTTGGAGAGGAGAAAATTGTTGTGTGGAATTTGGAGTTGGAATCAGGAAATTGTGCTTTTATTTGAGTTATGAGTTTGCTGAGTATAAAATGGAGCTTTCCTTTGAGAATATTTTGGGAGTTGAGCTTCGTTGTCCGCTCGATCGACCTTCCAACCTTCTTCTCATTCAGGTTTGTTCTTCGTTTTTCTCTTCTACTGGTTCTACACTTTTACAACGAAAGATGACTTTGTCTTCATTTTTCTTCTCTCTTTTAACTTTTTAAAAACATTTTCGAATTATTGGTCGAAATTAGATTTTTAAGAATACTTTTTTAATGGAGTATATTACAAATAATAGTTTTCGAGAAATATATAGTGGGACAAAATTTTATTTGAAGGATTGTTTAGTACTAGCTCAACTTTTGAGTTTGTGTTTAAAAGATTTATAAACTTTAAAAAGTGTTTGATGTTTGATAGGTATATAAACTTTTAATTTTATGTTTAATAGGTCATTACTTGTTCAAAATTCTTAAAATTTAATTGATCTGATAGATATAAAATAGATTTGATGTCTAATAAGATCTTAAACTTTCATTTATTTTTTAATAAGTTTCTAAATTTTAAAAAATAATAGAAAATTTATTATTTTATTTTCTTTGCTATATATATGGGATGAGATTCAAGCGTCTAACTTTTTATCAAGCAACATGCTTTGTATCAGTTGTGCTATAGAAAAACTATTTAAAATAATAGAGAATCATCAAAAAGGTACATTTGAATCTCAATTAAAATCTAAAATTAATAATAATAGGTTAAATTACATGTTTAGTCCTTAAACTTTTAGGGTTGTGTCAATTTAGTCCCTGAACTTTAAAAAGTTTCAAATAGGTATTTGAACTTTCAATTTTGTTTCTAATAAATGCATAAACTTTAAAAAGTATCTCATAGATCCTTGAACTTTCATTTTTGTGTCAGTCTCTCAACTTTAATTATATGTCTAATAAATCATTGACATATTCAACATTTTTTAAAATTCACAAGTTTACTGGATACAAAAATTAGAAGTTTGGAGTCTCGATAGACATAAAATTCAATTATCAAATATATCAATAATCTATTAGACACTAAATTGAAAGTTTAGAATTTATTGGACATTTTTAAAGTCCATGGACTATGAGAAACAAAATTAAAAATTTAGGAATTTATTAAAAAAAAATTTAAGTTCAGGGAATACAAGGGACTCCATAATAATATTTATTACGTATCAAAATAGGTTTGTGTTAAACTATATTGATTTTTTTATCCAAAAAAAAAAAAAAAAAAAAACTATATTGATTTTGTTCGAATCGTTCAATTGGCAGTTGCAGGGAGCTCCACGGATTTTCAAGAAAAGTTCGTCCCCTTCTTCTTCTTCTTCTTCCAAGGAATCCACTGGTTCCCGTTGGATCAGAGCTGTCGATTTCACTCCATCTTCATGCATTGGACAATCCTTCGCTCTGTGCTTGGAAGTTTCTCCTGGAGATCAGCTTCTTTCTTTCTTCGAAACATTGGTCGGATACAAAGAAACCTATGGCCCATTCAATCTGGAGACAGGTTCTTCTTTCTCTTCCACTTCCAATCTGGTGCCTATTATTTCTCCACCGCAAGGCTTTGATATCTCTTATAAAATTTTGTTCAAAATCAATGCCCTGGTTCAACATGGTCACCTTCCTGGACCATTGTTGGATCATGAATTCTTCCGACTGGTCGATCCCAGTCGATTTCATCCTGACTTTGTCGAGCATGCCCTAGAGAAATTGTCTAATTTGAAGGAATGTTGCTATGAACCACAAAAATGGCTCAAGCAGCAATATATGTCATTCTACAACTCTAAGCAGCTTTCATGGAAACCTAATATCTCTTTGGAAGATGGCTTGGTCTATGTGCATAGGGTTCAAATTACACCTTCTAAAGTGTACTTTCGTGGTCCGGAGGCCAATCTTTCCAACAGAGTAGTACGTCGCTTTATCGATGATATAGATAATTTTCTTCGCGTGTCTTTTGTTGATGAGGAGTTGGATAAAATTCGTTCCATTGATCTATCTCCACGTACATCTACTGAAAATAGTGAAAGAACTAGCGTTTATGATAGAATACTATATGTTTTAAGAAATGGTATAGTCATTGGTGAGAAGAAGTTTGAGTTTCTTGCTTTCTCGGCGAGTCAATTAAGGGAAAATTCTTTTTGGATGTTTGCTTCGAGGGAGGGGCTGACTGCAGCAGATATCAGAGAGTGGATGGGTGACTTTCGTCAGATAAGGAATGTTGCAAAATATGCTGCTCGACTTGGTCAATCTTTTGGCTCGTCAAGAAAAACTCTGTGTGTTGAAGAGCATGAAATTGAAGTTATTCCTGATGTAGAAGTTGAAAGGAAGAAAACCGCGTATTGTTTCTCTGATGGGATTGGAAAAATATCTGAAACATTGGCTGAGAAGGTTGCTGAAAAATGTGGCTTGTTCAGTCATACACCATCTGCCTTCCAAATTCGGTATGCTGGATACAAAGGCGTTGTTGCTATCGACCCAACATCGAAAAAGAAATTGTCACTAAGAAAGAGTATGTTGAAGTATATGTCGCTTGACACCCAACTGGATGTTTTGTCTTGGAGCAGGTACCAACCCTGTTTTCTTAACCGTCAGGTGATCAACCTTCTGTCCACTCTTGGAATTCGGGATCGTGTTTTTGTGAAAAAACAAAAGGAGGCTATAGATCAACTAGATGCTATTCTAAAAGATCCATCAAAGGCATTAGAAGTATTGGAGTTGATTTCCCCAGGGGAAATGACTAGTATTCTGAAGGAAATGCTTTTATTTTACAAACCAAATGAAGAGCCTTTTCTAAATATGATGCTACGAACATTTCGAGCGGATAAATTGTTGGACCTGAGGACCAAATCAAGGATATTTGTTCCTAAGGGAAGGACAATGATGGGTTGCCTGGATGAAACCCGAACACTGGAATATGGACAGGTGTTTGTTCATTGTTCTGTCCCTAGCAGATCAAGCGAAAGTAATTTTGTGGTTACGGGTAAAGTAGTTGTTGCTAAAAATCCCTGTTTACACCCAGGGGATGTGCGTCTGCTCGATGCTGTTGATGTGAAAGCTCTGCACCATATGGTGGATTGTGTCGTTTTTCCACAGAAAGGAGAAAGGTAAGCAAGCTTATTGTGTATGAAATGTTTATGTATTTAACACATTTCACATGTCCATATTTGTTGCAATTTCATGCACTTCCCAGGTATTATATTTGCATGGAGCTCATGTCCTTAAGATTTATGGTAGGATTACTCACATATATTCATTAATTTACCATAAATCAGAATTTGGTTTTAGGTTATGGAACTAGATACTATTATCTTTATCTTGAAAATGTTAAGGACGTTGTTTTCTGTTTTTATCTTCAAAATTGCTTTTATTTTCTTGAAAATAGGTCATTTATGAGAAACAAGAAAAAAGAAATTTCAAAATAACAGTTATAAGAATTTCTTGTCGCAGATGACAGAAAACTATTTTAAAAATAGTTTACAAACAACCATAAGATTCCCACTATGCTTTGGTATGGTTGCTAAGCTATGCTATGCTGATTAATTATGTGAAGTTTATTGTTGGATAGAAATTCTTTTATGACTTTTTGTTGTCCTTATCTAATATTTCTAATATGTTATGTTAGACCTCACCCCAATGAATGCTCCGGGAGTGATCTGGATGGCGATTTATATTTTGTTTGTTGGGATTCGGAGCTGACTTGTATTAGACCAGCTAAACCTATGAGCTACAAGCCTGCACCAACTATGCAGTTGGATCATGATGTCACAATCGAGGTATGGTTTTAGGAAGTATTTACTTCTATTGCAATTTTTTTTTCTTGTTTTTATTTAAATGAGATGCATTGTAAATGAGATTTAGAAGTTTACGACAAATTCTACAACTAAGTTTGAATTATATACGATCATTTTGATACAGGAAGTGCAGGAATATTTGACCATGTACATGGTGAATGATGGTCCTGGAGGCATTGCAAATGCTCACACTGTCTATGCAGATAAGAAGCCTAAGAAGGCAATGAGTGCCGAATGTATTAAACTTGCAAAATTATTCTCAATTGCTGTTGACTTCCCGAAGACTGGTGTGCCAGCAAACTTGCCACGCTCTCTTCGTGTCCAGGAGTATCCAGATTTCATGGAAAAGCCCGATAAACCGACGTATGTATCGAATGGTGTTCTCGGAAAGCTTTTTCGAAGAGTCAAAGATGTTTCTTCTGACATGAACACCATCGAAACCTTCACTAAGGAAGTTGCTACCGAGTGCTACGATCCAGACATGGAAGTAGATGGTTTTGAAAATTATTTGAGAGAAGCTTTTGATTGCAAAAACAGGTACGACTTCAAGTTGGGGAACCTAATGGACTATTATGGTATCAAAACAGAACCTGAATTAGTCGGTGGAAATATTTTGAGAGTGGGAAAGTCTTTTGATAAAAGGAATGACTTGGAACAAATCAGCTTGGCCATGAAGTCACTAAGAAAGGAGGCCAGGGCTTGGTTTAATGAGAAGGGAAGTAAATCTACACATGACGAAAACGAAGAATTTGCAAAAGCATCTGCTTGGTACCATGTCACGTATCACCCAAATTATTGGGGTCGTTACAATGAAGGTATGCAAAGAGATCACTTTTTGAGTTTTCCTTGGTGTGTTTCTGACAAGCTTATTCAAATCAAGAGGGAGAAGAAGATGTGTAAGATAAGTTCTTCACAAGTGTCATCGCTGATACATAAATTTGGCAACCTGAGTTTATACTAACTTTAGGGGGTCAAGTAATGTTTTCCATTCTGGTTTCCCTACATATTTTTAGAACATAAAATATAATATGTTTGTAATGTAATGTATCTAGGTTTTAATTATCTTGGTCTTGTAATTTTATTAAGTATTTTAATAGCTTTTTAATGCTTGAAGTAATTGTGATGGGATGACCAGAAGATGGTTCAATGTCTGTGTCCTTGAGTTCTAAGATGATGGTGTGATGAGGTTGTAATTTCTATTTTACTACTTAAAGAAATGAATTTCTAGCTTTTTGTTTGAACTTTTATGCTTCATTAGATGTGGAAATGTA

mRNA sequence

ATGGCTACGATCGAGATTTTCGGATTTCCGGCAAGCGTCGCCGCCGACGATGTGAAGGAATTTCTGGAGAATCACACCGGCGACGGAACGGTGTCGACGGTGAGGATTTCGAAACCCAAAGACGAAAACTCCCGGTTCGCATTCGCGACGGTCCGGTTCACGAGCAAACTGGCGGCAGAGTACGTGGTGGCGCAGGCGGAGGCAGAGGAGCGGCTGTGGTTCGAAAGTTCGTATCTGAAAGCCAGAGGAGTGGAGAGGGAGATGAGGGCGGCGGCGGCGGAGGCAAGGAGGCGGGGCGAGGTGGAGAGAATCGAAAACGTGAGGATTCACTTGGGAAATCGGATTTCGAAGGAGAGAATGAGTGTGATTTGGAGAGGAGAAAATTGTTGTGTGGAATTTGGAGTTGGAATCAGGAAATTGTGCTTTTATTTGAGTTATGAGTTTGCTGAGTATAAAATGGAGCTTTCCTTTGAGAATATTTTGGGAGTTGAGCTTCGTTGTCCGCTCGATCGACCTTCCAACCTTCTTCTCATTCAGTTGCAGGGAGCTCCACGGATTTTCAAGAAAAGTTCGTCCCCTTCTTCTTCTTCTTCTTCCAAGGAATCCACTGGTTCCCGTTGGATCAGAGCTGTCGATTTCACTCCATCTTCATGCATTGGACAATCCTTCGCTCTGTGCTTGGAAGTTTCTCCTGGAGATCAGCTTCTTTCTTTCTTCGAAACATTGGTCGGATACAAAGAAACCTATGGCCCATTCAATCTGGAGACAGGTTCTTCTTTCTCTTCCACTTCCAATCTGGTGCCTATTATTTCTCCACCGCAAGGCTTTGATATCTCTTATAAAATTTTGTTCAAAATCAATGCCCTGGTTCAACATGGTCACCTTCCTGGACCATTGTTGGATCATGAATTCTTCCGACTGGTCGATCCCAGTCGATTTCATCCTGACTTTGTCGAGCATGCCCTAGAGAAATTGTCTAATTTGAAGGAATGTTGCTATGAACCACAAAAATGGCTCAAGCAGCAATATATGTCATTCTACAACTCTAAGCAGCTTTCATGGAAACCTAATATCTCTTTGGAAGATGGCTTGGTCTATGTGCATAGGGTTCAAATTACACCTTCTAAAGTGTACTTTCGTGGTCCGGAGGCCAATCTTTCCAACAGAGTAGTACGTCGCTTTATCGATGATATAGATAATTTTCTTCGCGTGTCTTTTGTTGATGAGGAGTTGGATAAAATTCGTTCCATTGATCTATCTCCACGTACATCTACTGAAAATAGTGAAAGAACTAGCGTTTATGATAGAATACTATATGTTTTAAGAAATGGTATAGTCATTGGTGAGAAGAAGTTTGAGTTTCTTGCTTTCTCGGCGAGTCAATTAAGGGAAAATTCTTTTTGGATGTTTGCTTCGAGGGAGGGGCTGACTGCAGCAGATATCAGAGAGTGGATGGGTGACTTTCGTCAGATAAGGAATGTTGCAAAATATGCTGCTCGACTTGGTCAATCTTTTGGCTCGTCAAGAAAAACTCTGTGTGTTGAAGAGCATGAAATTGAAGTTATTCCTGATGTAGAAGTTGAAAGGAAGAAAACCGCGTATTGTTTCTCTGATGGGATTGGAAAAATATCTGAAACATTGGCTGAGAAGGTTGCTGAAAAATGTGGCTTGTTCAGTCATACACCATCTGCCTTCCAAATTCGGTATGCTGGATACAAAGGCGTTGTTGCTATCGACCCAACATCGAAAAAGAAATTGTCACTAAGAAAGAGTATGTTGAAGTATATGTCGCTTGACACCCAACTGGATGTTTTGTCTTGGAGCAGGTACCAACCCTGTTTTCTTAACCGTCAGGTGATCAACCTTCTGTCCACTCTTGGAATTCGGGATCGTGTTTTTGTGAAAAAACAAAAGGAGGCTATAGATCAACTAGATGCTATTCTAAAAGATCCATCAAAGGCATTAGAAGTATTGGAGTTGATTTCCCCAGGGGAAATGACTAGTATTCTGAAGGAAATGCTTTTATTTTACAAACCAAATGAAGAGCCTTTTCTAAATATGATGCTACGAACATTTCGAGCGGATAAATTGTTGGACCTGAGGACCAAATCAAGGATATTTGTTCCTAAGGGAAGGACAATGATGGGTTGCCTGGATGAAACCCGAACACTGGAATATGGACAGGTGTTTGTTCATTGTTCTGTCCCTAGCAGATCAAGCGAAAGTAATTTTGTGGTTACGGGTAAAGTAGTTGTTGCTAAAAATCCCTGTTTACACCCAGGGGATGTGCGTCTGCTCGATGCTGTTGATGTGAAAGCTCTGCACCATATGGTGGATTGTGTCGTTTTTCCACAGAAAGGAGAAAGACCTCACCCCAATGAATGCTCCGGGAGTGATCTGGATGGCGATTTATATTTTGTTTGTTGGGATTCGGAGCTGACTTGTATTAGACCAGCTAAACCTATGAGCTACAAGCCTGCACCAACTATGCAGTTGGATCATGATGTCACAATCGAGGAAGTGCAGGAATATTTGACCATGTACATGGTGAATGATGGTCCTGGAGGCATTGCAAATGCTCACACTGTCTATGCAGATAAGAAGCCTAAGAAGGCAATGAGTGCCGAATGTATTAAACTTGCAAAATTATTCTCAATTGCTGTTGACTTCCCGAAGACTGGTGTGCCAGCAAACTTGCCACGCTCTCTTCGTGTCCAGGAGTATCCAGATTTCATGGAAAAGCCCGATAAACCGACGTATGTATCGAATGGTGTTCTCGGAAAGCTTTTTCGAAGAGTCAAAGATGTTTCTTCTGACATGAACACCATCGAAACCTTCACTAAGGAAGTTGCTACCGAGTGCTACGATCCAGACATGGAAGTAGATGGTTTTGAAAATTATTTGAGAGAAGCTTTTGATTGCAAAAACAGGTACGACTTCAAGTTGGGGAACCTAATGGACTATTATGGTATCAAAACAGAACCTGAATTAGTCGGTGGAAATATTTTGAGAGTGGGAAAGTCTTTTGATAAAAGGAATGACTTGGAACAAATCAGCTTGGCCATGAAGTCACTAAGAAAGGAGGCCAGGGCTTGGTTTAATGAGAAGGGAAGTAAATCTACACATGACGAAAACGAAGAATTTGCAAAAGCATCTGCTTGGTACCATGTCACGTATCACCCAAATTATTGGGGTCGTTACAATGAAGGTATGCAAAGAGATCACTTTTTGAGTTTTCCTTGGTGTGTTTCTGACAAGCTTATTCAAATCAAGAGGGAGAAGAAGATGTGTAAGATAAGTTCTTCACAAGTGTCATCGCTGATACATAAATTTGGCAACCTGAGTTTATACTAA

Coding sequence (CDS)

ATGGCTACGATCGAGATTTTCGGATTTCCGGCAAGCGTCGCCGCCGACGATGTGAAGGAATTTCTGGAGAATCACACCGGCGACGGAACGGTGTCGACGGTGAGGATTTCGAAACCCAAAGACGAAAACTCCCGGTTCGCATTCGCGACGGTCCGGTTCACGAGCAAACTGGCGGCAGAGTACGTGGTGGCGCAGGCGGAGGCAGAGGAGCGGCTGTGGTTCGAAAGTTCGTATCTGAAAGCCAGAGGAGTGGAGAGGGAGATGAGGGCGGCGGCGGCGGAGGCAAGGAGGCGGGGCGAGGTGGAGAGAATCGAAAACGTGAGGATTCACTTGGGAAATCGGATTTCGAAGGAGAGAATGAGTGTGATTTGGAGAGGAGAAAATTGTTGTGTGGAATTTGGAGTTGGAATCAGGAAATTGTGCTTTTATTTGAGTTATGAGTTTGCTGAGTATAAAATGGAGCTTTCCTTTGAGAATATTTTGGGAGTTGAGCTTCGTTGTCCGCTCGATCGACCTTCCAACCTTCTTCTCATTCAGTTGCAGGGAGCTCCACGGATTTTCAAGAAAAGTTCGTCCCCTTCTTCTTCTTCTTCTTCCAAGGAATCCACTGGTTCCCGTTGGATCAGAGCTGTCGATTTCACTCCATCTTCATGCATTGGACAATCCTTCGCTCTGTGCTTGGAAGTTTCTCCTGGAGATCAGCTTCTTTCTTTCTTCGAAACATTGGTCGGATACAAAGAAACCTATGGCCCATTCAATCTGGAGACAGGTTCTTCTTTCTCTTCCACTTCCAATCTGGTGCCTATTATTTCTCCACCGCAAGGCTTTGATATCTCTTATAAAATTTTGTTCAAAATCAATGCCCTGGTTCAACATGGTCACCTTCCTGGACCATTGTTGGATCATGAATTCTTCCGACTGGTCGATCCCAGTCGATTTCATCCTGACTTTGTCGAGCATGCCCTAGAGAAATTGTCTAATTTGAAGGAATGTTGCTATGAACCACAAAAATGGCTCAAGCAGCAATATATGTCATTCTACAACTCTAAGCAGCTTTCATGGAAACCTAATATCTCTTTGGAAGATGGCTTGGTCTATGTGCATAGGGTTCAAATTACACCTTCTAAAGTGTACTTTCGTGGTCCGGAGGCCAATCTTTCCAACAGAGTAGTACGTCGCTTTATCGATGATATAGATAATTTTCTTCGCGTGTCTTTTGTTGATGAGGAGTTGGATAAAATTCGTTCCATTGATCTATCTCCACGTACATCTACTGAAAATAGTGAAAGAACTAGCGTTTATGATAGAATACTATATGTTTTAAGAAATGGTATAGTCATTGGTGAGAAGAAGTTTGAGTTTCTTGCTTTCTCGGCGAGTCAATTAAGGGAAAATTCTTTTTGGATGTTTGCTTCGAGGGAGGGGCTGACTGCAGCAGATATCAGAGAGTGGATGGGTGACTTTCGTCAGATAAGGAATGTTGCAAAATATGCTGCTCGACTTGGTCAATCTTTTGGCTCGTCAAGAAAAACTCTGTGTGTTGAAGAGCATGAAATTGAAGTTATTCCTGATGTAGAAGTTGAAAGGAAGAAAACCGCGTATTGTTTCTCTGATGGGATTGGAAAAATATCTGAAACATTGGCTGAGAAGGTTGCTGAAAAATGTGGCTTGTTCAGTCATACACCATCTGCCTTCCAAATTCGGTATGCTGGATACAAAGGCGTTGTTGCTATCGACCCAACATCGAAAAAGAAATTGTCACTAAGAAAGAGTATGTTGAAGTATATGTCGCTTGACACCCAACTGGATGTTTTGTCTTGGAGCAGGTACCAACCCTGTTTTCTTAACCGTCAGGTGATCAACCTTCTGTCCACTCTTGGAATTCGGGATCGTGTTTTTGTGAAAAAACAAAAGGAGGCTATAGATCAACTAGATGCTATTCTAAAAGATCCATCAAAGGCATTAGAAGTATTGGAGTTGATTTCCCCAGGGGAAATGACTAGTATTCTGAAGGAAATGCTTTTATTTTACAAACCAAATGAAGAGCCTTTTCTAAATATGATGCTACGAACATTTCGAGCGGATAAATTGTTGGACCTGAGGACCAAATCAAGGATATTTGTTCCTAAGGGAAGGACAATGATGGGTTGCCTGGATGAAACCCGAACACTGGAATATGGACAGGTGTTTGTTCATTGTTCTGTCCCTAGCAGATCAAGCGAAAGTAATTTTGTGGTTACGGGTAAAGTAGTTGTTGCTAAAAATCCCTGTTTACACCCAGGGGATGTGCGTCTGCTCGATGCTGTTGATGTGAAAGCTCTGCACCATATGGTGGATTGTGTCGTTTTTCCACAGAAAGGAGAAAGACCTCACCCCAATGAATGCTCCGGGAGTGATCTGGATGGCGATTTATATTTTGTTTGTTGGGATTCGGAGCTGACTTGTATTAGACCAGCTAAACCTATGAGCTACAAGCCTGCACCAACTATGCAGTTGGATCATGATGTCACAATCGAGGAAGTGCAGGAATATTTGACCATGTACATGGTGAATGATGGTCCTGGAGGCATTGCAAATGCTCACACTGTCTATGCAGATAAGAAGCCTAAGAAGGCAATGAGTGCCGAATGTATTAAACTTGCAAAATTATTCTCAATTGCTGTTGACTTCCCGAAGACTGGTGTGCCAGCAAACTTGCCACGCTCTCTTCGTGTCCAGGAGTATCCAGATTTCATGGAAAAGCCCGATAAACCGACGTATGTATCGAATGGTGTTCTCGGAAAGCTTTTTCGAAGAGTCAAAGATGTTTCTTCTGACATGAACACCATCGAAACCTTCACTAAGGAAGTTGCTACCGAGTGCTACGATCCAGACATGGAAGTAGATGGTTTTGAAAATTATTTGAGAGAAGCTTTTGATTGCAAAAACAGGTACGACTTCAAGTTGGGGAACCTAATGGACTATTATGGTATCAAAACAGAACCTGAATTAGTCGGTGGAAATATTTTGAGAGTGGGAAAGTCTTTTGATAAAAGGAATGACTTGGAACAAATCAGCTTGGCCATGAAGTCACTAAGAAAGGAGGCCAGGGCTTGGTTTAATGAGAAGGGAAGTAAATCTACACATGACGAAAACGAAGAATTTGCAAAAGCATCTGCTTGGTACCATGTCACGTATCACCCAAATTATTGGGGTCGTTACAATGAAGGTATGCAAAGAGATCACTTTTTGAGTTTTCCTTGGTGTGTTTCTGACAAGCTTATTCAAATCAAGAGGGAGAAGAAGATGTGTAAGATAAGTTCTTCACAAGTGTCATCGCTGATACATAAATTTGGCAACCTGAGTTTATACTAA

Protein sequence

MATIEIFGFPASVAADDVKEFLENHTGDGTVSTVRISKPKDENSRFAFATVRFTSKLAAEYVVAQAEAEERLWFESSYLKARGVEREMRAAAAEARRRGEVERIENVRIHLGNRISKERMSVIWRGENCCVEFGVGIRKLCFYLSYEFAEYKMELSFENILGVELRCPLDRPSNLLLIQLQGAPRIFKKSSSPSSSSSSKESTGSRWIRAVDFTPSSCIGQSFALCLEVSPGDQLLSFFETLVGYKETYGPFNLETGSSFSSTSNLVPIISPPQGFDISYKILFKINALVQHGHLPGPLLDHEFFRLVDPSRFHPDFVEHALEKLSNLKECCYEPQKWLKQQYMSFYNSKQLSWKPNISLEDGLVYVHRVQITPSKVYFRGPEANLSNRVVRRFIDDIDNFLRVSFVDEELDKIRSIDLSPRTSTENSERTSVYDRILYVLRNGIVIGEKKFEFLAFSASQLRENSFWMFASREGLTAADIREWMGDFRQIRNVAKYAARLGQSFGSSRKTLCVEEHEIEVIPDVEVERKKTAYCFSDGIGKISETLAEKVAEKCGLFSHTPSAFQIRYAGYKGVVAIDPTSKKKLSLRKSMLKYMSLDTQLDVLSWSRYQPCFLNRQVINLLSTLGIRDRVFVKKQKEAIDQLDAILKDPSKALEVLELISPGEMTSILKEMLLFYKPNEEPFLNMMLRTFRADKLLDLRTKSRIFVPKGRTMMGCLDETRTLEYGQVFVHCSVPSRSSESNFVVTGKVVVAKNPCLHPGDVRLLDAVDVKALHHMVDCVVFPQKGERPHPNECSGSDLDGDLYFVCWDSELTCIRPAKPMSYKPAPTMQLDHDVTIEEVQEYLTMYMVNDGPGGIANAHTVYADKKPKKAMSAECIKLAKLFSIAVDFPKTGVPANLPRSLRVQEYPDFMEKPDKPTYVSNGVLGKLFRRVKDVSSDMNTIETFTKEVATECYDPDMEVDGFENYLREAFDCKNRYDFKLGNLMDYYGIKTEPELVGGNILRVGKSFDKRNDLEQISLAMKSLRKEARAWFNEKGSKSTHDENEEFAKASAWYHVTYHPNYWGRYNEGMQRDHFLSFPWCVSDKLIQIKREKKMCKISSSQVSSLIHKFGNLSLY
Homology
BLAST of Spg008341 vs. NCBI nr
Match: XP_038875555.1 (probable RNA-dependent RNA polymerase 1 [Benincasa hispida])

HSP 1 Score: 1885.5 bits (4883), Expect = 0.0e+00
Identity = 938/1121 (83.68%), Postives = 1011/1121 (90.19%), Query Frame = 0

Query: 3    TIEIFGFPASVAADDVKEFLENHTGDGTVSTVRISKPKDENSRFAFATVRFTSKLAAEYV 62
            TIEI+GF   V  D+VKEFLENHTGDGTV TVRISKPKDE +RF F TVRF S+LAAEY+
Sbjct: 4    TIEIYGFRPEVTVDEVKEFLENHTGDGTVLTVRISKPKDEKTRFTFVTVRFKSELAAEYI 63

Query: 63   VAQA-EAEERLWFESSYLKARGVEREMRAAAAEARRRGEVERIENVRIHLGNRISKERMS 122
            V +A  AE++LWF SSYLKAR VER     A+ AR  GE+ER+E+V+ HLG+ ISK++M 
Sbjct: 64   VEKATAAEKKLWFGSSYLKAREVER-----ASPARGGGEMERMEDVKGHLGSLISKDKMR 123

Query: 123  VIWRGENCCVEFGVGIRKLCFYLSYEFAEYKMELSFENILGVELRCPLDRPSNLLLIQLQ 182
            VIW GEN  VEFG+G+RKL FYLSYE  EYKMEL+FENILGVE RCPL++PS   LIQLQ
Sbjct: 124  VIWEGENWNVEFGIGVRKLSFYLSYE-VEYKMELAFENILGVEFRCPLNQPSKFFLIQLQ 183

Query: 183  GAPRIFKKSSSPSSSSSSKESTGSRWIRAVDFTPSSCIGQSFALCLEVSPGDQLLSFFET 242
            GAPRIFKKS S SSS  +      RWIR VDFTPSSCIGQSFALCLE+SPG  L  FF+ 
Sbjct: 184  GAPRIFKKSRSSSSSRLNSNEFCFRWIRDVDFTPSSCIGQSFALCLELSPGHHLPPFFQN 243

Query: 243  LVGYKETYGPFNLETGSSFSSTSNLVPIISPPQGFDISYKILFKINALVQHGHLPGPLLD 302
            LVGYKETY PF L+TGSSFSS SNLVPII+PP+GFDISYK LFKINALVQHG+LPGP LD
Sbjct: 244  LVGYKETYAPFILQTGSSFSSISNLVPIITPPRGFDISYKTLFKINALVQHGYLPGPTLD 303

Query: 303  HEFFRLVDPSRFHPDFVEHALEKLSNLKECCYEPQKWLKQQYMSFYNSKQLSWKPNISLE 362
             EFFRLVD SRF PD+VEHALEK+ NLKECCYEPQKWLK  Y+SFY S QL WKPNISL+
Sbjct: 304  DEFFRLVDSSRFRPDYVEHALEKMFNLKECCYEPQKWLKHHYLSFYTSNQLPWKPNISLD 363

Query: 363  DGLVYVHRVQITPSKVYFRGPEANLSNRVVRRFIDDIDNFLRVSFVDEELDKIRSIDLSP 422
            DGLVYVHRVQITPSKVYFRGPEANLSNRVVRRFIDDIDNFLRVSFVDE+LDK+ SIDL+P
Sbjct: 364  DGLVYVHRVQITPSKVYFRGPEANLSNRVVRRFIDDIDNFLRVSFVDEDLDKLHSIDLAP 423

Query: 423  RTSTE-NSERTSVYDRILYVLRNGIVIGEKKFEFLAFSASQLRENSFWMFASREGLTAAD 482
            R+S+  NSERT VYDRIL VLRNGIVIG+KKFEFLAFSASQLRENSFWMFASREGL+AAD
Sbjct: 424  RSSSAGNSERTRVYDRILSVLRNGIVIGDKKFEFLAFSASQLRENSFWMFASREGLSAAD 483

Query: 483  IREWMGDFRQIRNVAKYAARLGQSFGSSRKTLCVEEHEIEVIPDVEVERKKTAYCFSDGI 542
            IREWMGDFR+IRNVAKYAARLGQSFGSSRKTLCVEEHE EVIPDVEVERKKT YCFSDGI
Sbjct: 484  IREWMGDFREIRNVAKYAARLGQSFGSSRKTLCVEEHEFEVIPDVEVERKKTKYCFSDGI 543

Query: 543  GKISETLAEKVAEKCGLFSHTPSAFQIRYAGYKGVVAIDPTSKKKLSLRKSMLKYMSLDT 602
            GKIS+TLAEKVAEKCGL SHTPSAFQIRYAGYKGVVAIDPT KKKLSLRKSMLKYMSLDT
Sbjct: 544  GKISKTLAEKVAEKCGLISHTPSAFQIRYAGYKGVVAIDPTLKKKLSLRKSMLKYMSLDT 603

Query: 603  QLDVLSWSRYQPCFLNRQVINLLSTLGIRDRVFVKKQKEAIDQLDAILKDPSKALEVLEL 662
            QLDVL WS+YQPCFLNRQVINLLSTLGI+DRVFVKKQKEAIDQLD+IL+DPSKALEVLEL
Sbjct: 604  QLDVLLWSKYQPCFLNRQVINLLSTLGIKDRVFVKKQKEAIDQLDSILEDPSKALEVLEL 663

Query: 663  ISPGEMTSILKEMLLFYKPNEEPFLNMMLRTFRADKLLDLRTKSRIFVPKGRTMMGCLDE 722
            +SPGEMT ILKE+LLFYKPNEEPFLNMMLRTFRADK LDLRTKSRIFVPKGRTMMGCLDE
Sbjct: 664  MSPGEMTGILKELLLFYKPNEEPFLNMMLRTFRADKFLDLRTKSRIFVPKGRTMMGCLDE 723

Query: 723  TRTLEYGQVFVHCSVPSRSSESNFVVTGKVVVAKNPCLHPGDVRLLDAVDVKALHHMVDC 782
            TRTLEYGQVFVHCS+P RS+ESNFV+ GKVVVAKNPCLHPGDVR+LDAVDVKALHHMVDC
Sbjct: 724  TRTLEYGQVFVHCSIPGRSNESNFVIKGKVVVAKNPCLHPGDVRVLDAVDVKALHHMVDC 783

Query: 783  VVFPQKGERPHPNECSGSDLDGDLYFVCWDSELTCIRPAKPMSYKPAPTMQLDHDVTIEE 842
            VVFPQKG+RPHPNECSGSDLDGDLYFVCWD ELTCI+P KPMSY+PAPTMQLDHDVTIEE
Sbjct: 784  VVFPQKGKRPHPNECSGSDLDGDLYFVCWDPELTCIKPVKPMSYEPAPTMQLDHDVTIEE 843

Query: 843  VQEYLTMYMVNDGPGGIANAHTVYADKKPKKAMSAECIKLAKLFSIAVDFPKTGVPANLP 902
            VQEYL  YMVNDG G IANAHTV+ADK PKKAMS ECIKLAKLFSIAVDFPKTGVPANLP
Sbjct: 844  VQEYLANYMVNDGLGAIANAHTVFADKNPKKAMSTECIKLAKLFSIAVDFPKTGVPANLP 903

Query: 903  RSLRVQEYPDFMEKPDKPTYVSNGVLGKLFRRVKDVSSDMNTIETFTKEVATECYDPDME 962
            R+LRV EYPDFM+KP+KPTY SNGVLGKLF+ VKDVSSD+NT+E FT+EVA  CYDPDME
Sbjct: 904  RNLRVHEYPDFMDKPNKPTYASNGVLGKLFQGVKDVSSDVNTLEIFTREVAINCYDPDME 963

Query: 963  VDGFENYLREAFDCKNRYDFKLGNLMDYYGIKTEPELVGGNILRVGKSFDKRNDLEQISL 1022
            VDGF+ YL EAFD K RYDFKLGNLMDYYGIKTEPELV GNILR+ KSFDKRNDLEQI+ 
Sbjct: 964  VDGFDKYLSEAFDYKTRYDFKLGNLMDYYGIKTEPELVSGNILRMAKSFDKRNDLEQITF 1023

Query: 1023 AMKSLRKEARAWFNEKGSKSTHD----ENEEFAKASAWYHVTYHPNYWGRYNEGMQRDHF 1082
            AMKSLRKEARAWFNEKGSKST+D    E EE+AKASAWYHVTYHP+YWGRYNEGMQRDHF
Sbjct: 1024 AMKSLRKEARAWFNEKGSKSTYDNSKGEEEEYAKASAWYHVTYHPDYWGRYNEGMQRDHF 1083

Query: 1083 LSFPWCVSDKLIQIKREKKMCKISSSQVSSLIHKFGNLSLY 1118
            LSFPWCV+DKLIQIKREK +  +SSS +SSLIHKFG+LSLY
Sbjct: 1084 LSFPWCVADKLIQIKREKTLL-MSSSPMSSLIHKFGSLSLY 1117

BLAST of Spg008341 vs. NCBI nr
Match: KAG6579193.1 (RNA-dependent RNA polymerase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1863.6 bits (4826), Expect = 0.0e+00
Identity = 925/1097 (84.32%), Postives = 1000/1097 (91.16%), Query Frame = 0

Query: 3    TIEIFGFPASVAADDVKEFLENHTGDGTVSTVRISKPKDENSRFAFATVRFTSKLAAEYV 62
            TIEI+GFPA V AD+VKEF+ENHTGDGTV TVRISK  DE +RF FATVRFTSKL  EYV
Sbjct: 10   TIEIYGFPAEVTADEVKEFIENHTGDGTVLTVRISKFNDEKARFTFATVRFTSKLGGEYV 69

Query: 63   VAQAEAEERLWFESSYLKARGVEREMRAAAAEARRRGEVERIENVRIHLGNRISKERMSV 122
            VAQA AE+RLWF SSYLKAR VERE++ AAA AR  GE+ER+ENV +  G+ +SKE+M V
Sbjct: 70   VAQAAAEKRLWFGSSYLKARKVEREIKTAAA-ARVHGELERMENVNVQWGSLVSKEKMKV 129

Query: 123  IWRGENCCVEFGVGIRKLCFYLSYEFAEYKMELSFENILGVELRCPLDRPSNLLLIQLQG 182
            IW+G    VE+G+G+RKL FYLSYE  EYKMEL FENILGV+LRC +DR S L LIQLQG
Sbjct: 130  IWKGGKWSVEYGIGVRKLGFYLSYEGVEYKMELCFENILGVQLRCSVDRGSKLFLIQLQG 189

Query: 183  APRIFKKSS-SPSSSSSSKESTGSRWIRAVDFTPSSCIGQSFALCLEVSPGDQLLSFFET 242
            APRIFKK+  S SS  SS+ESTG RWIR VDFTPSSCIGQSFALCLE+S GDQL SFF+T
Sbjct: 190  APRIFKKAPLSSSSRLSSEESTGFRWIRDVDFTPSSCIGQSFALCLELSHGDQLPSFFQT 249

Query: 243  LVGYKETYGPFNLETGSSFSSTSNLVPIISPPQGFDISYKILFKINALVQHGHLPGPLLD 302
            LVGYKE+Y PF L+TGSS SS SNLVPII+PPQGFDI YKILFKINAL+QHG+LPGP LD
Sbjct: 250  LVGYKESYAPFILQTGSSLSSISNLVPIITPPQGFDIPYKILFKINALLQHGYLPGPALD 309

Query: 303  HEFFRLVDPSRFHPDFVEHALEKLSNLKECCYEPQKWLKQQYMSFYNSKQLSWKPNISLE 362
            +EFFRLVD SRF PD+VE+AL+KL +LKECCYEPQKWLKQQY+SFY+SKQL WKPNISL+
Sbjct: 310  NEFFRLVDSSRFRPDYVEYALDKLFHLKECCYEPQKWLKQQYLSFYSSKQLPWKPNISLD 369

Query: 363  DGLVYVHRVQITPSKVYFRGPEANLSNRVVRRFIDDIDNFLRVSFVDEELDKIRSIDLSP 422
            DGLVYVHRVQITP+KVYFRGPEANLSNRVVR FIDD+DNFLRVSFVDEELDK+ SIDLSP
Sbjct: 370  DGLVYVHRVQITPTKVYFRGPEANLSNRVVRHFIDDVDNFLRVSFVDEELDKLHSIDLSP 429

Query: 423  RTSTENSERTSVYDRILYVLRNGIVIGEKKFEFLAFSASQLRENSFWMFASREGLTAADI 482
            RTS EN  RT VYDR+L VLRNGIVIG+KKFEFLAFSASQLRENS WMFASR+GL+AADI
Sbjct: 430  RTSAENDLRTRVYDRVLSVLRNGIVIGDKKFEFLAFSASQLRENSCWMFASRKGLSAADI 489

Query: 483  REWMGDFRQIRNVAKYAARLGQSFGSSRKTLCVEEHEIEVIPDVEVERKKTAYCFSDGIG 542
            REWMGD RQIRNVAKYAARLGQSFGSSR+TLCVE+HEIEVI DVEVE  K  YCFSDGIG
Sbjct: 490  REWMGDLRQIRNVAKYAARLGQSFGSSRRTLCVEQHEIEVISDVEVETNKITYCFSDGIG 549

Query: 543  KISETLAEKVAEKCGLFSHTPSAFQIRYAGYKGVVAIDPTSKKKLSLRKSMLKYMSLDTQ 602
            KIS TLAEKVAEKCGL SHTPSAFQIRYAGYKGVVAIDPTS KKLSLRKSMLKY SLDTQ
Sbjct: 550  KISGTLAEKVAEKCGLISHTPSAFQIRYAGYKGVVAIDPTSTKKLSLRKSMLKYTSLDTQ 609

Query: 603  LDVLSWSRYQPCFLNRQVINLLSTLGIRDRVFVKKQKEAIDQLDAILKDPSKALEVLELI 662
            +DVLSWS+YQPCFLNRQVINLLSTLGI+DRVFVKKQKEAIDQLD+IL+DPS+ALEVLEL+
Sbjct: 610  VDVLSWSKYQPCFLNRQVINLLSTLGIKDRVFVKKQKEAIDQLDSILEDPSRALEVLELM 669

Query: 663  SPGEMTSILKEMLLFYKPNEEPFLNMMLRTFRADKLLDLRTKSRIFVPKGRTMMGCLDET 722
            SPGEMTSILKE+LL YKPNEEPFLNMMLRTFRA KLLDLRTKSRIFVPKGRTMMGCLDET
Sbjct: 670  SPGEMTSILKELLLLYKPNEEPFLNMMLRTFRAAKLLDLRTKSRIFVPKGRTMMGCLDET 729

Query: 723  RTLEYGQVFVHCSVPSRSSESNFVVTGKVVVAKNPCLHPGDVRLLDAVDVKALHHMVDCV 782
            RTLEYGQVFVHCS+P RSSESNFVV GKVVVAKNPCLHPGDVRLLDAVDVKALHHMVDCV
Sbjct: 730  RTLEYGQVFVHCSLPGRSSESNFVVKGKVVVAKNPCLHPGDVRLLDAVDVKALHHMVDCV 789

Query: 783  VFPQKGERPHPNECSGSDLDGDLYFVCWDSELTCIRPAKPMSYKPAPTMQLDHDVTIEEV 842
            VFPQKGERPHPNECSGSDLDGDLYFV WD ELT I+P KPMSY+PAPTMQLDHDVTIEEV
Sbjct: 790  VFPQKGERPHPNECSGSDLDGDLYFVGWDPELTRIKPVKPMSYEPAPTMQLDHDVTIEEV 849

Query: 843  QEYLTMYMVNDGPGGIANAHTVYADKKPKKAMSAECIKLAKLFSIAVDFPKTGVPANLPR 902
            Q YL  YMVNDG GGIANAHTV+ADKKPKKAM+AECIKLAKLFSIAVDFPKTGVPAN P 
Sbjct: 850  QGYLVKYMVNDGLGGIANAHTVFADKKPKKAMTAECIKLAKLFSIAVDFPKTGVPANFPH 909

Query: 903  SLRVQEYPDFMEKPDKPTYVSNGVLGKLFRRVKDVSSDMNTIETFTKEVATECYDPDMEV 962
            +LRV+EYPDFMEKPDKPTYVSNGVLGKLFR VKDVSSD+NT+E F++EVAT+CYDPDMEV
Sbjct: 910  NLRVREYPDFMEKPDKPTYVSNGVLGKLFRGVKDVSSDVNTVEIFSREVATKCYDPDMEV 969

Query: 963  DGFENYLREAFDCKNRYDFKLGNLMDYYGIKTEPELVGGNILRVGKSFDKRNDLEQISLA 1022
            DGFE+YL EAF+ K RYDFKLGNLMDYYGIKTEPEL+ GNILR+ KSFDKRND+EQISLA
Sbjct: 970  DGFEDYLSEAFEFKARYDFKLGNLMDYYGIKTEPELISGNILRMAKSFDKRNDMEQISLA 1029

Query: 1023 MKSLRKEARAWFNEKGSKSTHDE----NEEFAKASAWYHVTYHPNYWGRYNEGMQRDHFL 1082
            MKSLRKEAR+WFNEKGSKS +DE    NEE+AKASAWY VTYHP+YWGRYNEGMQRDHFL
Sbjct: 1030 MKSLRKEARSWFNEKGSKSAYDEDDNKNEEYAKASAWYRVTYHPDYWGRYNEGMQRDHFL 1089

Query: 1083 SFPWCVSDKLIQIKREK 1095
            SFPWCVSDKLI+IKREK
Sbjct: 1090 SFPWCVSDKLIRIKREK 1105

BLAST of Spg008341 vs. NCBI nr
Match: XP_023549735.1 (probable RNA-dependent RNA polymerase 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1857.0 bits (4809), Expect = 0.0e+00
Identity = 924/1107 (83.47%), Postives = 1001/1107 (90.42%), Query Frame = 0

Query: 3    TIEIFGFPASVAADDVKEFLENHTGDGTVSTVRISKPKDENSRFAFATVRFTSKLAAEYV 62
            TIEIFGFPA V AD+VKEF+ENHTGDGTV TVRISKP DE +RF FATVRFTSKL  EYV
Sbjct: 10   TIEIFGFPAQVTADEVKEFIENHTGDGTVLTVRISKPNDEKARFTFATVRFTSKLGGEYV 69

Query: 63   VAQAEAEERLWFESSYLKARGVEREMRAAAAEARRRGEVERIENVRIHLGNRISKERMSV 122
            VAQA AE+RLWF S YLKAR VERE++  AA AR  GE+ER+ENV +  GN +SKE M V
Sbjct: 70   VAQAAAEKRLWFGSLYLKARKVEREIKTTAA-ARVHGELERMENVNVQWGNLVSKEEMKV 129

Query: 123  IWRGENCCVEFGVGIRKLCFYLSYEFAEYKMELSFENILGVELRCPLDRPSNLLLIQLQG 182
            IW+GE   VE+G+G+RKL FYLSYE  EYKMEL FENILGV+LRC  DR S   LIQLQG
Sbjct: 130  IWKGEKWSVEYGIGVRKLGFYLSYEGVEYKMELCFENILGVQLRCSDDRGSKHFLIQLQG 189

Query: 183  APRIFKKSS-SPSSSSSSKESTGSRWIRAVDFTPSSCIGQSFALCLEVSPGDQLLSFFET 242
            APRIFKK+  S SS  SS+ESTG RWIR VDFTPSSCIGQSFALCLE+S GDQL SFF+T
Sbjct: 190  APRIFKKAPLSSSSRLSSEESTGFRWIRDVDFTPSSCIGQSFALCLELSHGDQLPSFFQT 249

Query: 243  LVGYKETYGPFNLETGSSFSSTSNLVPIISPPQGFDISYKILFKINALVQHGHLPGPLLD 302
            LVGYKE+Y PF L TGSS SS SNLVPII+PPQGF ISYKILF+INAL+QHG+LPGP LD
Sbjct: 250  LVGYKESYAPFILHTGSSLSSISNLVPIITPPQGFHISYKILFQINALLQHGYLPGPALD 309

Query: 303  HEFFRLVDPSRFHPDFVEHALEKLSNLKECCYEPQKWLKQQYMSFYNSKQLSWKPNISLE 362
            +EFFRLVDPSRF  D+VE+AL+KL +LKECCYEPQKWLKQQY+SFY+SKQL WKPN+SL+
Sbjct: 310  NEFFRLVDPSRFRSDYVEYALDKLFHLKECCYEPQKWLKQQYLSFYSSKQLPWKPNVSLD 369

Query: 363  DGLVYVHRVQITPSKVYFRGPEANLSNRVVRRFIDDIDNFLRVSFVDEELDKIRSIDLSP 422
            DGLVYVHRVQITP+KVYFRGPEANLSNRVVR FIDD+DNFLRVSFVDEELDK+ SIDLSP
Sbjct: 370  DGLVYVHRVQITPTKVYFRGPEANLSNRVVRHFIDDVDNFLRVSFVDEELDKLHSIDLSP 429

Query: 423  RTSTENSERTSVYDRILYVLRNGIVIGEKKFEFLAFSASQLRENSFWMFASREGLTAADI 482
            RTS EN  RT VYDR+L VLRNGIVIG+KKFEFLAFSASQLRENS WMFASR+GL+AADI
Sbjct: 430  RTSAENDLRTRVYDRVLSVLRNGIVIGDKKFEFLAFSASQLRENSCWMFASRKGLSAADI 489

Query: 483  REWMGDFRQIRNVAKYAARLGQSFGSSRKTLCVEEHEIEVIPDVEVERKKTAYCFSDGIG 542
            R WMGDFRQIRNVAKYAARLGQSFGSSR+TLCVE+HEIEVI DVEVE  K  YCFSDGIG
Sbjct: 490  RAWMGDFRQIRNVAKYAARLGQSFGSSRRTLCVEQHEIEVISDVEVETNKITYCFSDGIG 549

Query: 543  KISETLAEKVAEKCGLFSHTPSAFQIRYAGYKGVVAIDPTSKKKLSLRKSMLKYMSLDTQ 602
            KIS TLAEKVAEKCGL SHTPSAFQIRYAGYKGVVAIDPTS KKLSLRKSMLKY SLDTQ
Sbjct: 550  KISGTLAEKVAEKCGLISHTPSAFQIRYAGYKGVVAIDPTSTKKLSLRKSMLKYTSLDTQ 609

Query: 603  LDVLSWSRYQPCFLNRQVINLLSTLGIRDRVFVKKQKEAIDQLDAILKDPSKALEVLELI 662
            +DVLSWS+YQPCFLNRQVINLLSTLGI+DRVFVKKQKEAIDQLD+IL+DPS+ALEVLEL+
Sbjct: 610  VDVLSWSKYQPCFLNRQVINLLSTLGIKDRVFVKKQKEAIDQLDSILEDPSRALEVLELM 669

Query: 663  SPGEMTSILKEMLLFYKPNEEPFLNMMLRTFRADKLLDLRTKSRIFVPKGRTMMGCLDET 722
            SPGEMTSILKE+LL YKPNEEPFLNMMLRTFRA KLLDLRTKSRIFVPKGRTMMGCLDET
Sbjct: 670  SPGEMTSILKELLLLYKPNEEPFLNMMLRTFRAAKLLDLRTKSRIFVPKGRTMMGCLDET 729

Query: 723  RTLEYGQVFVHCSVPSRSSESNFVVTGKVVVAKNPCLHPGDVRLLDAVDVKALHHMVDCV 782
            RTLEYGQVFVHCS+P RSSESNFVV GKVVVAKNPCLHPGDVRLLDAVDVKALHHMVDCV
Sbjct: 730  RTLEYGQVFVHCSLPGRSSESNFVVKGKVVVAKNPCLHPGDVRLLDAVDVKALHHMVDCV 789

Query: 783  VFPQKGERPHPNECSGSDLDGDLYFVCWDSELTCIRPAKPMSYKPAPTMQLDHDVTIEEV 842
            VFPQKGERPHPNECSGSDLDGDLYFV WD ELT I+P KPMSY+PAPTM+LDHDVT+ EV
Sbjct: 790  VFPQKGERPHPNECSGSDLDGDLYFVGWDPELTRIKPVKPMSYEPAPTMRLDHDVTMGEV 849

Query: 843  QEYLTMYMVNDGPGGIANAHTVYADKKPKKAMSAECIKLAKLFSIAVDFPKTGVPANLPR 902
            QEYL  YMVNDG GGIANAHTV+AD+KPKKAM+AECIKLAKLFSIAVDFPKTGVPAN P 
Sbjct: 850  QEYLVKYMVNDGLGGIANAHTVFADQKPKKAMTAECIKLAKLFSIAVDFPKTGVPANFPH 909

Query: 903  SLRVQEYPDFMEKPDKPTYVSNGVLGKLFRRVKDVSSDMNTIETFTKEVATECYDPDMEV 962
            +LRV+EYPDFMEKPDKPTYVSNGVLGKLFR VKDVSSD+NT+E F++EVAT+CYDPDMEV
Sbjct: 910  NLRVREYPDFMEKPDKPTYVSNGVLGKLFRGVKDVSSDVNTVEIFSREVATKCYDPDMEV 969

Query: 963  DGFENYLREAFDCKNRYDFKLGNLMDYYGIKTEPELVGGNILRVGKSFDKRNDLEQISLA 1022
            DGFE+YL EAF+ K RYDFKLGNLMDYYGIKTEPEL+ GNILR+ KSFDKRND+EQISLA
Sbjct: 970  DGFEDYLSEAFEYKARYDFKLGNLMDYYGIKTEPELISGNILRMAKSFDKRNDMEQISLA 1029

Query: 1023 MKSLRKEARAWFNEKGSKSTHDE----NEEFAKASAWYHVTYHPNYWGRYNEGMQRDHFL 1082
            MKSLRKEAR+WFNEKGSKS +DE    +EE+AKASAWY VTYHP+YWGRYNEGMQRDHFL
Sbjct: 1030 MKSLRKEARSWFNEKGSKSAYDEDDNKDEEYAKASAWYRVTYHPDYWGRYNEGMQRDHFL 1089

Query: 1083 SFPWCVSDKLIQIKREKKMCKISSSQV 1105
            SFPWCVSDKLIQIKRE K C ++ S V
Sbjct: 1090 SFPWCVSDKLIQIKRE-KTCLVNFSPV 1114

BLAST of Spg008341 vs. NCBI nr
Match: XP_022938807.1 (probable RNA-dependent RNA polymerase 1 [Cucurbita moschata])

HSP 1 Score: 1855.9 bits (4806), Expect = 0.0e+00
Identity = 923/1107 (83.38%), Postives = 1000/1107 (90.33%), Query Frame = 0

Query: 3    TIEIFGFPASVAADDVKEFLENHTGDGTVSTVRISKPKDENSRFAFATVRFTSKLAAEYV 62
            TIEI+GFP  V AD+VKEF+ENHTGDGTV TVRISK  DE +RF FATV+FTSKL  EYV
Sbjct: 10   TIEIYGFPTQVTADEVKEFIENHTGDGTVLTVRISKLNDEKARFTFATVQFTSKLGGEYV 69

Query: 63   VAQAEAEERLWFESSYLKARGVEREMRAAAAEARRRGEVERIENVRIHLGNRISKERMSV 122
            VAQA AE+RLWF SSYLKAR VERE++ A   AR  GE+ER+ENV +  G+ +SKE M V
Sbjct: 70   VAQAAAEKRLWFGSSYLKARKVEREIKTA---ARVHGELERMENVNVQWGSLVSKEEMKV 129

Query: 123  IWRGENCCVEFGVGIRKLCFYLSYEFAEYKMELSFENILGVELRCPLDRPSNLLLIQLQG 182
            IW+G    VE+G+G+RKL FYLSYE  EYKMEL FENILGV+LRC +DR S L LIQLQG
Sbjct: 130  IWKGGKWSVEYGIGVRKLGFYLSYEGVEYKMELCFENILGVQLRCSVDRGSKLFLIQLQG 189

Query: 183  APRIFKKSS-SPSSSSSSKESTGSRWIRAVDFTPSSCIGQSFALCLEVSPGDQLLSFFET 242
            APRIFKK+  S SS  SS+ESTG RWIR VDFTPSSCIGQSFALCLE+S GDQL SFF+T
Sbjct: 190  APRIFKKAPLSSSSRLSSEESTGFRWIRDVDFTPSSCIGQSFALCLELSHGDQLPSFFQT 249

Query: 243  LVGYKETYGPFNLETGSSFSSTSNLVPIISPPQGFDISYKILFKINALVQHGHLPGPLLD 302
            LVGYKE+Y PF L+TGSS SS SNLVPII+PPQGFDI YKILFKINAL+QHG+LPGP LD
Sbjct: 250  LVGYKESYAPFILQTGSSLSSISNLVPIITPPQGFDIPYKILFKINALLQHGYLPGPALD 309

Query: 303  HEFFRLVDPSRFHPDFVEHALEKLSNLKECCYEPQKWLKQQYMSFYNSKQLSWKPNISLE 362
            +EFFRLVD SRF PD+VE+AL+KL + KECCYEPQKWLKQQY+SFY+SKQL WKPNISL+
Sbjct: 310  NEFFRLVDSSRFRPDYVEYALDKLFHFKECCYEPQKWLKQQYLSFYSSKQLPWKPNISLD 369

Query: 363  DGLVYVHRVQITPSKVYFRGPEANLSNRVVRRFIDDIDNFLRVSFVDEELDKIRSIDLSP 422
            DGLVYVHRVQITP+KVYFRGPEANLSNRVVR FIDD+DNFLRVSFVDEELDK+ SIDLSP
Sbjct: 370  DGLVYVHRVQITPTKVYFRGPEANLSNRVVRHFIDDVDNFLRVSFVDEELDKLHSIDLSP 429

Query: 423  RTSTENSERTSVYDRILYVLRNGIVIGEKKFEFLAFSASQLRENSFWMFASREGLTAADI 482
            RTS EN  RT VYDR+L VLRNGIVIG+KKFEFLAFSASQLRENS WMFASR+GL+AADI
Sbjct: 430  RTSAENDLRTRVYDRVLSVLRNGIVIGDKKFEFLAFSASQLRENSCWMFASRKGLSAADI 489

Query: 483  REWMGDFRQIRNVAKYAARLGQSFGSSRKTLCVEEHEIEVIPDVEVERKKTAYCFSDGIG 542
            REWMGD RQIRNVAKYAARLGQSFGSSR+TLCVE+HEIEVI DVEVE  K  YCFSDGIG
Sbjct: 490  REWMGDLRQIRNVAKYAARLGQSFGSSRRTLCVEQHEIEVISDVEVETNKITYCFSDGIG 549

Query: 543  KISETLAEKVAEKCGLFSHTPSAFQIRYAGYKGVVAIDPTSKKKLSLRKSMLKYMSLDTQ 602
            KIS TLAEKVAEKCGL SHTPSAFQIRYAGYKGVVAIDPTS KKLSLRKSMLKY SLDTQ
Sbjct: 550  KISGTLAEKVAEKCGLISHTPSAFQIRYAGYKGVVAIDPTSTKKLSLRKSMLKYTSLDTQ 609

Query: 603  LDVLSWSRYQPCFLNRQVINLLSTLGIRDRVFVKKQKEAIDQLDAILKDPSKALEVLELI 662
            +DVLSWS+YQPCFLNRQVINLLSTLGI+DRVFVKKQKEAIDQLD+IL+DPS+ALEVLEL+
Sbjct: 610  VDVLSWSKYQPCFLNRQVINLLSTLGIKDRVFVKKQKEAIDQLDSILEDPSRALEVLELM 669

Query: 663  SPGEMTSILKEMLLFYKPNEEPFLNMMLRTFRADKLLDLRTKSRIFVPKGRTMMGCLDET 722
            SPGEMTSILKE+LL YKPNEEPFLNMMLRTFRA KLLDLRTKSRIFVPKGRTMMGCLDET
Sbjct: 670  SPGEMTSILKELLLLYKPNEEPFLNMMLRTFRAAKLLDLRTKSRIFVPKGRTMMGCLDET 729

Query: 723  RTLEYGQVFVHCSVPSRSSESNFVVTGKVVVAKNPCLHPGDVRLLDAVDVKALHHMVDCV 782
            RTLEYGQVFVHCS+P RSSESNFVV GKVVVAKNPCLHPGDVRLLDAVDVKALHHMVDCV
Sbjct: 730  RTLEYGQVFVHCSLPGRSSESNFVVKGKVVVAKNPCLHPGDVRLLDAVDVKALHHMVDCV 789

Query: 783  VFPQKGERPHPNECSGSDLDGDLYFVCWDSELTCIRPAKPMSYKPAPTMQLDHDVTIEEV 842
            VFPQKG+RPHPNECSGSDLDGDLYFV WD ELT I+P KPMSY+PAPTMQLDHDVTIEEV
Sbjct: 790  VFPQKGKRPHPNECSGSDLDGDLYFVGWDPELTRIKPVKPMSYEPAPTMQLDHDVTIEEV 849

Query: 843  QEYLTMYMVNDGPGGIANAHTVYADKKPKKAMSAECIKLAKLFSIAVDFPKTGVPANLPR 902
            Q YL  YMVNDG GGIANAHTV+ADKKPKKAM+AECIKLAKLFSIAVDFPKTGVPAN P 
Sbjct: 850  QGYLVKYMVNDGLGGIANAHTVFADKKPKKAMAAECIKLAKLFSIAVDFPKTGVPANFPH 909

Query: 903  SLRVQEYPDFMEKPDKPTYVSNGVLGKLFRRVKDVSSDMNTIETFTKEVATECYDPDMEV 962
            +LRV+EYPDFMEKPDKPTYVSNGVLGKLFR VKDVSSD+NT+E F++EVAT+CYDPDMEV
Sbjct: 910  NLRVREYPDFMEKPDKPTYVSNGVLGKLFRGVKDVSSDVNTVEIFSREVATKCYDPDMEV 969

Query: 963  DGFENYLREAFDCKNRYDFKLGNLMDYYGIKTEPELVGGNILRVGKSFDKRNDLEQISLA 1022
            DGFE+YL EAF+ K RYDFKLGNLMDYYGIKTEPEL+ GNILR+ KSFDKRND+EQISLA
Sbjct: 970  DGFEDYLSEAFEFKARYDFKLGNLMDYYGIKTEPELISGNILRMAKSFDKRNDMEQISLA 1029

Query: 1023 MKSLRKEARAWFNEKGSKSTHDE----NEEFAKASAWYHVTYHPNYWGRYNEGMQRDHFL 1082
            MKSLRKEAR+WFNEKGSKS +DE    NEE+AKASAWY VTYHP+YWGRYNEGMQRDHFL
Sbjct: 1030 MKSLRKEARSWFNEKGSKSAYDEDDNKNEEYAKASAWYRVTYHPDYWGRYNEGMQRDHFL 1089

Query: 1083 SFPWCVSDKLIQIKREKKMCKISSSQV 1105
            SFPWCVSDKLIQIKRE K C ++ S V
Sbjct: 1090 SFPWCVSDKLIQIKRE-KTCLVNFSPV 1112

BLAST of Spg008341 vs. NCBI nr
Match: XP_022994004.1 (probable RNA-dependent RNA polymerase 1 [Cucurbita maxima])

HSP 1 Score: 1855.5 bits (4805), Expect = 0.0e+00
Identity = 926/1107 (83.65%), Postives = 1001/1107 (90.42%), Query Frame = 0

Query: 4    IEIFGFPASVAADDVKEFLENHTGDGTVSTVRISKPKDENSRFAFATVRFTSKLAAEYVV 63
            IEI+GFPA V AD+VKEF+ENHTGDGTV TVRISKP DE +RF FATVRFTSKL  EYVV
Sbjct: 11   IEIYGFPAEVTADEVKEFIENHTGDGTVLTVRISKPNDEKARFTFATVRFTSKLGGEYVV 70

Query: 64   AQAEAEERLWFESSYLKARGVEREMR--AAAAEARRRGEVERIENVRIHLGNRISKERMS 123
            AQA AE+RLWF SSYLKAR VERE+R   AAA AR  G +ER+ENV +  G+ +SKE+M 
Sbjct: 71   AQAAAEKRLWFGSSYLKARKVEREIRTATAAAAARVHGGLERMENVNVQWGSLVSKEKMK 130

Query: 124  VIWRGENCCVEFGVGIRKLCFYLSYEFAEYKMELSFENILGVELRCPLDRPSNLLLIQLQ 183
            VIW+     VE+G+G RKL FYLSYE  EYKMEL FENILGV+LRC  DR S L LIQLQ
Sbjct: 131  VIWKEGKWSVEYGIGARKLGFYLSYEGVEYKMELCFENILGVQLRCSGDRGSELFLIQLQ 190

Query: 184  GAPRIFKKSSSPSSSS-SSKESTGSRWIRAVDFTPSSCIGQSFALCLEVSPGDQLLSFFE 243
            GAPRIFKK+ S SSS  SS+ESTG RWIR VDFTPSSCIGQSFALCLE+SPGDQL SFF 
Sbjct: 191  GAPRIFKKTPSSSSSRLSSEESTGFRWIRDVDFTPSSCIGQSFALCLELSPGDQLPSFFR 250

Query: 244  TLVGYKETYGPFNLETGSSFSSTSNLVPIISPPQGFDISYKILFKINALVQHGHLPGPLL 303
            TLVGYKE+Y PF L  GSS SS SNLVPII+PPQGFDISYKILFKINAL+QHG+LPGP L
Sbjct: 251  TLVGYKESYAPFILHPGSSLSSISNLVPIITPPQGFDISYKILFKINALLQHGYLPGPAL 310

Query: 304  DHEFFRLVDPSRFHPDFVEHALEKLSNLKECCYEPQKWLKQQYMSFYNSKQLSWKPNISL 363
            D+EFFRLVD SRF P+++E+ALEKL + KECCYEPQKWLKQQY+SFY+SKQL  KPN+SL
Sbjct: 311  DNEFFRLVDSSRFRPEYIEYALEKLFHFKECCYEPQKWLKQQYLSFYSSKQLPCKPNVSL 370

Query: 364  EDGLVYVHRVQITPSKVYFRGPEANLSNRVVRRFIDDIDNFLRVSFVDEELDKIRSIDLS 423
            +DGLVYVHRVQITP+KVYFRGPEANLSNRVVR FIDD+DNFLRVSFVDEELDK+ SIDLS
Sbjct: 371  DDGLVYVHRVQITPTKVYFRGPEANLSNRVVRHFIDDVDNFLRVSFVDEELDKLHSIDLS 430

Query: 424  PRTSTENSERTSVYDRILYVLRNGIVIGEKKFEFLAFSASQLRENSFWMFASREGLTAAD 483
            PRTS EN  RT VYDR+L VLRNGIVIG+KKFEFLAFSASQLRENS WMFASR+GL+AAD
Sbjct: 431  PRTSAENDLRTRVYDRVLSVLRNGIVIGDKKFEFLAFSASQLRENSCWMFASRKGLSAAD 490

Query: 484  IREWMGDFRQIRNVAKYAARLGQSFGSSRKTLCVEEHEIEVIPDVEVERKKTAYCFSDGI 543
            IREWMGDFRQIRNVAKYAARLGQSFGSSR+TLCVE+HEIEVIPDVEVE  K  YCFSDGI
Sbjct: 491  IREWMGDFRQIRNVAKYAARLGQSFGSSRRTLCVEQHEIEVIPDVEVETNKITYCFSDGI 550

Query: 544  GKISETLAEKVAEKCGLFSHTPSAFQIRYAGYKGVVAIDPTSKKKLSLRKSMLKYMSLDT 603
            GKIS TLAEKVAEKCGL SHTPSAFQIRYAGYKGVVAIDPTS KKLSLRKSMLKY SLDT
Sbjct: 551  GKISGTLAEKVAEKCGLISHTPSAFQIRYAGYKGVVAIDPTSTKKLSLRKSMLKYTSLDT 610

Query: 604  QLDVLSWSRYQPCFLNRQVINLLSTLGIRDRVFVKKQKEAIDQLDAILKDPSKALEVLEL 663
            Q+DVLSWS+YQPCFLNRQVINLLSTLGI+DRVFVKKQKEAIDQLD+IL+DPS+ALEVLEL
Sbjct: 611  QVDVLSWSKYQPCFLNRQVINLLSTLGIKDRVFVKKQKEAIDQLDSILEDPSRALEVLEL 670

Query: 664  ISPGEMTSILKEMLLFYKPNEEPFLNMMLRTFRADKLLDLRTKSRIFVPKGRTMMGCLDE 723
            +SPGEMTSILKE+LL YKPNEEPFLNMMLRTFRA KLLDLRTKSRIFVPKGRTMMGCLDE
Sbjct: 671  MSPGEMTSILKELLLLYKPNEEPFLNMMLRTFRAAKLLDLRTKSRIFVPKGRTMMGCLDE 730

Query: 724  TRTLEYGQVFVHCSVPSRSSESNFVVTGKVVVAKNPCLHPGDVRLLDAVDVKALHHMVDC 783
            TRTLEYGQVFVHCS+P RSSESNFVV GKVVVAKNPCLHPGDVRLLDAVDVKALHHMV+C
Sbjct: 731  TRTLEYGQVFVHCSLPGRSSESNFVVKGKVVVAKNPCLHPGDVRLLDAVDVKALHHMVNC 790

Query: 784  VVFPQKGERPHPNECSGSDLDGDLYFVCWDSELTCIRPAKPMSYKPAPTMQLDHDVTIEE 843
            VVFPQKGERPHPNECSGSDLDGDLYFV WD ELT I+P KPMSY+PAPTM+LDHDVTIEE
Sbjct: 791  VVFPQKGERPHPNECSGSDLDGDLYFVGWDPELTRIKPVKPMSYEPAPTMRLDHDVTIEE 850

Query: 844  VQEYLTMYMVNDGPGGIANAHTVYADKKPKKAMSAECIKLAKLFSIAVDFPKTGVPANLP 903
            VQEYL  YMVNDG GGIANAHTV+ADKKPKKAM+AECIKLAKLFSIAVDFPKTGVPAN P
Sbjct: 851  VQEYLAKYMVNDGLGGIANAHTVFADKKPKKAMTAECIKLAKLFSIAVDFPKTGVPANFP 910

Query: 904  RSLRVQEYPDFMEKPDKPTYVSNGVLGKLFRRVKDVSSDMNTIETFTKEVATECYDPDME 963
             +LRV+EYPDFMEKPDKPTYVSNGVLGKLFR VKDVSSD+NTIE F++EVAT+CYDPDME
Sbjct: 911  HNLRVREYPDFMEKPDKPTYVSNGVLGKLFRGVKDVSSDVNTIEIFSREVATKCYDPDME 970

Query: 964  VDGFENYLREAFDCKNRYDFKLGNLMDYYGIKTEPELVGGNILRVGKSFDKRNDLEQISL 1023
            VDGFE+YL EAF+ K RYDFKLGNLMDYYGIKTEPEL+ GNILR+ KSFDKRNDLEQISL
Sbjct: 971  VDGFEDYLSEAFEYKARYDFKLGNLMDYYGIKTEPELISGNILRMAKSFDKRNDLEQISL 1030

Query: 1024 AMKSLRKEARAWFNEKGSKSTHDE---NEEFAKASAWYHVTYHPNYWGRYNEGMQRDHFL 1083
            A+KSLRKEAR+WFNEKGSKS  DE   +EE+AKASAWY VTYHP+YWGRYNEGMQRDHFL
Sbjct: 1031 AIKSLRKEARSWFNEKGSKSACDEDDKDEEYAKASAWYRVTYHPDYWGRYNEGMQRDHFL 1090

Query: 1084 SFPWCVSDKLIQIKREKKMCKISSSQV 1105
            SFPWCVSDKLIQIKRE K C ++ S +
Sbjct: 1091 SFPWCVSDKLIQIKRE-KTCLVNLSPI 1116

BLAST of Spg008341 vs. ExPASy Swiss-Prot
Match: Q9LQV2 (RNA-dependent RNA polymerase 1 OS=Arabidopsis thaliana OX=3702 GN=RDR1 PE=2 SV=1)

HSP 1 Score: 1245.0 bits (3220), Expect = 0.0e+00
Identity = 634/1098 (57.74%), Postives = 804/1098 (73.22%), Query Frame = 0

Query: 3    TIEIFGFPASVAADDVKEFLENHTGDGTVSTVRISKPKDENSRFAFATVRFTSKLAAEYV 62
            TI++FGFP  V+A++VK+FLE  TG GTV  +++ +PK    R  +A V+FTS+     +
Sbjct: 4    TIQVFGFPNGVSAEEVKKFLERLTGSGTVYAIKVRQPKKGGPR-VYAIVQFTSERHTRLI 63

Query: 63   VAQAEAEERLWFESSYLKARGVEREMRAAAAEARRRGEVERIENVRIHLGNRISKERMSV 122
            +    A ERL++  SYLKA  VE+++       + R  +  I  +++  G ++S ++   
Sbjct: 64   I--TAAAERLYYGRSYLKAFEVEQDI-----VPKPRASLHTISGLKMFFGCQVSTKKFLT 123

Query: 123  IWRGENCCVEFGVGIRKLCFYLSYEFAEYKMELSFENILGVELRCPLDRPSNLLLIQLQG 182
            +W  ++ CV FG+G+RKL F  S+   +Y++ELS+ENI  ++L  P  R S  L+IQ+ G
Sbjct: 124  LWSAQDVCVSFGIGMRKLHFSFSWYQKDYRLELSYENIWQIDLHSPQGRSSKFLVIQVIG 183

Query: 183  APRIFKKSSSPSS-----SSSSKESTGSRWIRAVDFTPSSCIGQSFALCLEVSPGDQLLS 242
            AP+IF+K   P +          + +  +WIR  DFT SSCIGQS A CLE+     +  
Sbjct: 184  APKIFEKEDQPINLLFGIMDFYSDGSDEQWIRTTDFTSSSCIGQSTAFCLELPVHLNVPD 243

Query: 243  FFETLVGYKE-TYGPFNLETGSSFSSTSN-LVPIISPPQGFDISYKILFKINALVQHGHL 302
            F E    Y E     F +E+GSS+SS +N LVP++ PP GF + ++ILFK+N LVQ+  L
Sbjct: 244  FRENFANYAEHRASSFLIESGSSYSSNANTLVPVVDPPPGFSLPFEILFKLNTLVQNACL 303

Query: 303  PGPLLDHEFFRLVDPSRFHPDFVEHALEKLSNLKECCYEPQKWLKQQYMSFYNSKQLSWK 362
             GP LD +F+RL++  ++    ++H LEKL +L ECCYEP  WL+ +Y  + +  +L   
Sbjct: 304  SGPALDLDFYRLLNQKKYDRALIDHCLEKLFHLGECCYEPAHWLRDEYKKWISKGKLPLS 363

Query: 363  PNISLEDGLVYVHRVQITPSKVYFRGPEANLSNRVVRRFIDDIDNFLRVSFVDEELDKIR 422
            P ISL+DGLVY++RVQ+TP++VYF GPE N+SNRV+R +   I+NFLRVSFVDE+L+K+R
Sbjct: 364  PTISLDDGLVYMYRVQVTPARVYFSGPEVNVSNRVLRHYSKYINNFLRVSFVDEDLEKVR 423

Query: 423  SIDLSPRTSTENSERTSVYDRILYVLRNGIVIGEKKFEFLAFSASQLRENSFWMFASREG 482
            S+DLSPR+ST+   RT +YDRI  VLR+GIVIG+KKFEFLAFS+SQLRENS WMFA  + 
Sbjct: 424  SMDLSPRSSTQ--RRTKLYDRIYSVLRDGIVIGDKKFEFLAFSSSQLRENSAWMFAPIDR 483

Query: 483  LTAADIREWMGDFRQIRNVAKYAARLGQSFGSSRKTLCVEEHEIEVIPDVEVERKKTAYC 542
            +TAA IR WMGDF  IRNVAKYAARLGQSF SSR+TL V   EIEVIPDVE+    T Y 
Sbjct: 484  ITAAHIRAWMGDFDHIRNVAKYAARLGQSFSSSRETLNVRSDEIEVIPDVEIISLGTRYV 543

Query: 543  FSDGIGKISETLAEKVAEKCGLFSHTPSAFQIRYAGYKGVVAIDPTSKKKLSLRKSMLKY 602
            FSDGIGKIS   A KVA KCGL   +PSAFQIRY GYKGVVA+DP S KKLSLRKSM K+
Sbjct: 544  FSDGIGKISAEFARKVARKCGLTEFSPSAFQIRYGGYKGVVAVDPNSSKKLSLRKSMSKF 603

Query: 603  MSLDTQLDVLSWSRYQPCFLNRQVINLLSTLGIRDRVFVKKQKEAIDQLDAILKDPSKAL 662
             S +T+LDVL+WS+YQPC++NRQ+I LLSTLG+ D VF KKQ+E +D+LDAIL  P +A 
Sbjct: 604  ESENTKLDVLAWSKYQPCYMNRQLITLLSTLGVTDSVFEKKQREVVDRLDAILTHPLEAH 663

Query: 663  EVLELISPGEMTSILKEMLLF-YKPNEEPFLNMMLRTFRADKLLDLRTKSRIFVPKGRTM 722
            E L L++PGE T+ILK ++L  YKP+ EPFL+MML+ FRA KLL+LRTK+RIF+  GR+M
Sbjct: 664  EALGLMAPGENTNILKALILCGYKPDAEPFLSMMLQNFRASKLLELRTKTRIFISGGRSM 723

Query: 723  MGCLDETRTLEYGQVFVHCSVPSRSSESNFVVTGKVVVAKNPCLHPGDVRLLDAVDVKAL 782
            MGCLDETRTLEYGQV V  S P R     F++TG VVVAKNPCLHPGDVR+L AV+V AL
Sbjct: 724  MGCLDETRTLEYGQVVVQYSDPMRPGR-RFIITGPVVVAKNPCLHPGDVRVLQAVNVPAL 783

Query: 783  HHMVDCVVFPQKGERPHPNECSGSDLDGDLYFVCWDSELTCIRPAKPMSYKPAPTMQLDH 842
            +HMVDCVVFPQKG RPHPNECSGSDLDGD+YFVCWD EL   R ++PM Y P PT  LDH
Sbjct: 784  NHMVDCVVFPQKGLRPHPNECSGSDLDGDIYFVCWDQELVPPRTSEPMDYTPEPTQILDH 843

Query: 843  DVTIEEVQEYLTMYMVNDGPGGIANAHTVYADKKPKKAMSAECIKLAKLFSIAVDFPKTG 902
            DVTIEEV+EY   Y+VND  G IANAHT +ADK+P KA S  CI+LAK FS AVDFPKTG
Sbjct: 844  DVTIEEVEEYFANYIVNDSLGIIANAHTAFADKEPLKAFSDPCIELAKKFSTAVDFPKTG 903

Query: 903  VPANLPRSLRVQEYPDFMEKPDKPTYVSNGVLGKLFRRVKDVSSDMNTIETFTKEVATEC 962
            V A +P+ L V+EYPDFMEKPDKPTY S  V+GKLFR VK+ +  + +I++FT +VA++ 
Sbjct: 904  VAAVIPQHLYVKEYPDFMEKPDKPTYESKNVIGKLFREVKERAPPLISIKSFTLDVASKS 963

Query: 963  YDPDMEVDGFENYLREAFDCKNRYDFKLGNLMDYYGIKTEPELVGGNILRVGKSFDKRND 1022
            YD DMEVDGFE Y+ EAF  K  YDFKLGNLMDYYGIKTE E++ G I+R+ KSF KR D
Sbjct: 964  YDKDMEVDGFEEYVDEAFYQKANYDFKLGNLMDYYGIKTEAEILSGGIMRMSKSFTKRRD 1023

Query: 1023 LEQISLAMKSLRKEARAWFNEKGSKSTHDENEEFAKASAWYHVTYHPNYWGRYNEGMQRD 1082
             E I  A+++LRKE  + FN     ++ +E  E AKASAWYHVTYH +YWG YNEG+ RD
Sbjct: 1024 AESIGRAVRALRKETLSLFN-----ASEEEENESAKASAWYHVTYHSSYWGLYNEGLNRD 1083

Query: 1083 HFLSFPWCVSDKLIQIKR 1093
            HFLSF WCV DKL++IK+
Sbjct: 1084 HFLSFAWCVYDKLVRIKK 1085

BLAST of Spg008341 vs. ExPASy Swiss-Prot
Match: Q0DXS3 (Probable RNA-dependent RNA polymerase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=RDR1 PE=2 SV=2)

HSP 1 Score: 909.8 bits (2350), Expect = 3.0e-263
Identity = 452/736 (61.41%), Postives = 570/736 (77.45%), Query Frame = 0

Query: 375  SKVYFRGPEANLSNRVVRRFIDDIDNFLRVSFVDEELDKIRSIDLSPRT-STENSERTSV 434
            S VYF GPE N+SNRVVR F  DI+NFLR+SFVDE+ +K+R+ DLSPR+ S  ++ RT++
Sbjct: 7    STVYFYGPEINVSNRVVRNFSSDIENFLRISFVDEDCEKLRATDLSPRSASGHDANRTAL 66

Query: 435  YDRILYVLRNGIVIGEKKFEFLAFSASQLRENSFWMFASREGLTAADIREWMGDFRQIRN 494
            Y R+L VL +GI IG K FEFLAFS+SQLR+NS WMFASR+GL A+DIR WMGDFR IRN
Sbjct: 67   YKRVLSVLSDGITIGGKNFEFLAFSSSQLRDNSAWMFASRQGLAASDIRTWMGDFRNIRN 126

Query: 495  VAKYAARLGQSFGSSRKTLCVEEHEIEVIPDVEVERKKTAYCFSDGIGKISETLAEKVAE 554
            VAKYAARLGQSF SS +TL V+++E+E I D+   +  T + FSDGIGKIS   A +VA 
Sbjct: 127  VAKYAARLGQSFSSSTETLKVQKYEVEEISDI---KNGTQHVFSDGIGKISSAFANEVAM 186

Query: 555  KCGLFSHTPSAFQIRYAGYKGVVAIDPTSKKKLSLRKSMLKYMSLDTQLDVLSWSRYQPC 614
            KC L    PSAFQIRY GYKGVVA+DPTS+ KLSLRKSMLK+ S +  +DVL++S+YQP 
Sbjct: 187  KCNLKRFAPSAFQIRYGGYKGVVAVDPTSRWKLSLRKSMLKFQSDNITVDVLAYSKYQPG 246

Query: 615  FLNRQVINLLSTLGIRDRVFVKKQKEAIDQLDAILKDPSKALEVLELISPGEMTSILKEM 674
            FLNRQ+I LLSTLG+RD VF +KQ+EA++QL+ ++ DP  A+E +EL+  GE+T+ +KE+
Sbjct: 247  FLNRQLITLLSTLGVRDSVFEQKQEEAVNQLNKMVTDPQAAIEAIELMPMGEITNAVKEL 306

Query: 675  LLF-YKPNEEPFLNMMLRTFRADKLLDLRTKSRIFVPKGRTMMGCLDETRTLEYGQVFVH 734
            LL  Y+P++EP+L+M+L+TFRA KLL+L+TKSRI +PKGR MMGCLDETRTL+YGQVF+ 
Sbjct: 307  LLCGYQPDDEPYLSMLLQTFRASKLLELKTKSRILIPKGRAMMGCLDETRTLKYGQVFIR 366

Query: 735  CSVPSRSSESNFVVTGKVVVAKNPCLHPGDVRLLDAVDVKALHHMVDCVVFPQKGERPHP 794
             +    +    F VTGKVV+AKNPCLHPGD+R+L AVDV  LHHM +CVVFPQ+G RPHP
Sbjct: 367  -ATSGVNDNDRFTVTGKVVIAKNPCLHPGDIRILHAVDVPVLHHMFNCVVFPQQGPRPHP 426

Query: 795  NECSGSDLDGDLYFVCWDSELTCIRPAKPMSYKPAPTMQLDHDVTIEEVQEYLTMYMVND 854
            NECSGSDLDGD+YFV WD  L   R   PM Y PAPT  LDHDVTIEEV+EY T Y+VN+
Sbjct: 427  NECSGSDLDGDIYFVSWDPSLIPPRMVTPMDYTPAPTETLDHDVTIEEVEEYFTNYIVNE 486

Query: 855  GPGGIANAHTVYADKKPKKAMSAECIKLAKLFSIAVDFPKTGVPANLPRSLRVQEYPDFM 914
              G IANAH V+ADK+  KA S+ CI+LAKLFSIAVDFPKTGVPA +P  L V+EYPDFM
Sbjct: 487  SLGMIANAHVVFADKEDLKAESSPCIELAKLFSIAVDFPKTGVPALIPPELHVKEYPDFM 546

Query: 915  EKPDKPTYVSNGVLGKLFRRVKDVSSDMNTIETFTKEVATECYDPDMEVDGFENYLREAF 974
            EK DK TY S GV+GKL+R +K  +     I+ FT+EVA   YD DM VDG+E+Y+ EA 
Sbjct: 547  EKLDKVTYESKGVIGKLYREIKKHTPH---IKHFTREVARRSYDTDMIVDGYEDYITEAM 606

Query: 975  DCKNRYDFKLGNLMDYYGIKTEPELVGGNILRVGKSFDKRNDLEQISLAMKSLRKEARAW 1034
              K+ YDFKLGNLMD+YGIK+E E++ G IL++ K+F K++D + I LA++SLRKEAR+ 
Sbjct: 607  ALKDEYDFKLGNLMDHYGIKSEAEIISGCILKMAKNFTKKSDADAIRLAVRSLRKEARSR 666

Query: 1035 FNEKG-SKSTHDENEEFAKASAWYHVTYHPNYWGRYNEGMQRDHFLSFPWCVSDKLIQIK 1094
            F+E     + H  +   AKASAWYHVTYHP +WG YNEG +R HF+SFPWC+ +KL++IK
Sbjct: 667  FSEMSLDDNGHGHDASEAKASAWYHVTYHPEFWGCYNEGYERPHFISFPWCIYEKLLRIK 726

Query: 1095 REKKMCKISSSQVSSL 1108
            + +K  +    ++ SL
Sbjct: 727  QRRKFVRKMQPELFSL 735

BLAST of Spg008341 vs. ExPASy Swiss-Prot
Match: O82504 (RNA-dependent RNA polymerase 2 OS=Arabidopsis thaliana OX=3702 GN=RDR2 PE=1 SV=1)

HSP 1 Score: 752.3 bits (1941), Expect = 7.9e-216
Identity = 449/1149 (39.08%), Postives = 661/1149 (57.53%), Query Frame = 0

Query: 2    ATIEIFGFPASVAADDVKEFLENHTGDGTVSTVRISKPKDENSRFAFATVRFTSKLAAEY 61
            +T++I   P ++ AD++  FLE H G+ TV  + I   +D      FA V+FT+ L  + 
Sbjct: 10   STVKISNVPQTIVADELLRFLELHLGEDTVFALEIPTTRDNWKPRDFARVQFTT-LEVKS 69

Query: 62   VVAQAEAEERLWFESSYLKARGVEREMRAAAAEARRRGEVERIENVRIHLGNRISKERMS 121
                  ++ +L F++  L+      ++     + R+     R++++ + +G   S E+  
Sbjct: 70   RAQLLSSQSKLLFKTHNLRLSEAYDDIIPRPVDPRK-----RLDDIVLTVGFPESDEKRF 129

Query: 122  VI---WRGENCCVEFGVGIRKLCFYLSYEFAEYKMELSFENILGVELRCPLDRPSNL--L 181
                 W G  C +      R++ F++      YK+E+ FE+I+     C     S +   
Sbjct: 130  CALEKWDGVRCWIL--TEKRRVEFWVWESGDCYKIEVRFEDIIETLSCCVNGDASEIDAF 189

Query: 182  LIQLQGAPRIFKKSSSPSSSSSS-------KESTGSRWIRAVDFTPSSCIGQSFALCLEV 241
            L++L+  P++FK+ +   ++          KE     WIR  DF+ S  IG S   CLEV
Sbjct: 190  LLKLKYGPKVFKRVTVHIATKFKSDRYRFCKEDFDFMWIRTTDFSGSKSIGTSTCFCLEV 249

Query: 242  SPGDQLLSFFETLVGYKETYGPFNLETGSSFSSTSNLVPIISPP-QGFDISYKILFKINA 301
              G  +L  F  L  Y+E         G +F+S + +VP+++    G +  Y+ILF++NA
Sbjct: 250  HNGSTMLDIFSGLPYYREDTLSLTYVDGKTFASAAQIVPLLNAAILGLEFPYEILFQLNA 309

Query: 302  LVQHGHLP-GPLLDHEFFRLVDPSRFHPDFVEHALEKLSNLKECCYEPQKWLKQQYMSFY 361
            LV    +      D E  +++         V   L+KL      CY+P  ++K Q  S  
Sbjct: 310  LVHAQKISLFAASDMELIKILRGMSLETALV--ILKKLHQQSSICYDPVFFVKTQMQSVV 369

Query: 362  NSKQLSWKPNIS----LEDGLVYVHRVQITPSKVYFRGPEANLSNRVVRRFIDDIDNFLR 421
              K++   P  +     E  ++   R  +TPSK+Y  GPE   +N VV+ F + + +F+R
Sbjct: 370  --KKMKHSPASAYKRLTEQNIMSCQRAYVTPSKIYLLGPELETANYVVKNFAEHVSDFMR 429

Query: 422  VSFVDEELDKIRSIDLSPRTST---ENSERTSVYDRILYVLRNGIVIGEKKFEFLAFSAS 481
            V+FV+E+  K+ +  LS  +         RT++Y+R+L +L  GI +G K+FEFLAFSAS
Sbjct: 430  VTFVEEDWSKLPANALSVNSKEGYFVKPSRTNIYNRVLSILGEGITVGPKRFEFLAFSAS 489

Query: 482  QLRENSFWMFASREGLTAADIREWMGDFRQIRNVAKYAARLGQSFGSSRKTLCVEEHEIE 541
            QLR NS WMFAS E + A DIREWMG FR+IR+++K AAR+GQ F +SR+TL V   ++E
Sbjct: 490  QLRGNSVWMFASNEKVKAEDIREWMGCFRKIRSISKCAARMGQLFSASRQTLIVRAQDVE 549

Query: 542  VIPDVEVERKKTAYCFSDGIGKISETLAEKVAEKCGLFSHTPSAFQIRYAGYKGVVAIDP 601
             IPD+EV      YCFSDGIGKIS   A++VA+KCGL SH PSAFQIRY GYKGV+A+D 
Sbjct: 550  QIPDIEVTTDGADYCFSDGIGKISLAFAKQVAQKCGL-SHVPSAFQIRYGGYKGVIAVDR 609

Query: 602  TSKKKLSLRKSMLKYMSLDTQLDVLSWSRYQPCFLNRQVINLLSTLGIRDRVFVKKQKEA 661
            +S +KLSLR SMLK+ S +  L+V  W+   PCFLNR++I LLSTLGI D +F   Q   
Sbjct: 610  SSFRKLSLRDSMLKFDSNNRMLNVTRWTESMPCFLNREIICLLSTLGIEDAMFEAMQAVH 669

Query: 662  IDQLDAILKDPSKALEVLELISPGEMTSILKEMLL-FYKPNEEPFLNMMLRTFRADKLLD 721
            +  L  +L+D   AL VL+ +S     ++L +MLL  Y P+ EP+L+MMLR     +L +
Sbjct: 670  LSMLGNMLEDRDAALNVLQKLSGENSKNLLVKMLLQGYAPSSEPYLSMMLRVHHESQLSE 729

Query: 722  LRTKSRIFVPKGRTMMGCLDETRTLEYGQVFVHCSVPS------------RSSESNFVVT 781
            L+++ RI VPKGR ++GC+DE   LEYGQV+V  ++              +  E   VV 
Sbjct: 730  LKSRCRILVPKGRILIGCMDEMGILEYGQVYVRVTLTKAELKSRDQSYFRKIDEETSVVI 789

Query: 782  GKVVVAKNPCLHPGDVRLLDAVDVKALHH--MVDCVVFPQKGERPHPNECSGSDLDGDLY 841
            GKVVV KNPCLHPGD+R+LDA+          +DC++FPQKGERPHPNECSG DLDGD +
Sbjct: 790  GKVVVTKNPCLHPGDIRVLDAIYEVHFEEKGYLDCIIFPQKGERPHPNECSGGDLDGDQF 849

Query: 842  FVCWDSELTCIRPAKPMSYKPAPTMQLDHDVTIEEVQEYLTMYMVNDGPGGIANAHTVYA 901
            FV WD ++       PM Y  +    +DHDVT+EE+ ++   YM++D  G I+ AH V+A
Sbjct: 850  FVSWDEKIIPSEMDPPMDYAGSRPRLMDHDVTLEEIHKFFVDYMISDTLGVISTAHLVHA 909

Query: 902  DKKPKKAMSAECIKLAKLFSIAVDFPKTGVPANLPRSLRVQEYPDFMEKPDKPTYVSNGV 961
            D+ P+KA S +C++LA L S AVDF KTG PA +P +L+ +E+PDF+E+ +KPTY+S  V
Sbjct: 910  DRDPEKARSQKCLELANLHSRAVDFAKTGAPAEMPYALKPREFPDFLERFEKPTYISESV 969

Query: 962  LGKLFRRVKDVSSDMNTIETFTKEVATECYDPDMEVDGFENYLREAFDCKNRYDFKLGNL 1021
             GKL+R VK   S +   +   +   T  YD  +E  GFE+++  A   ++ Y  KL +L
Sbjct: 970  FGKLYRAVK---SSLAQRKPEAESEDTVAYDVTLEEAGFESFIETAKAHRDMYGEKLTSL 1029

Query: 1022 MDYYGIKTEPELVGGNILRVGKSFDKRND------LEQISLAMKSLRKEARAWFNEKGSK 1081
            M YYG   E E++ G IL+  + +  R++       ++I+L++K L KEA  WF     K
Sbjct: 1030 MIYYGAANEEEILTG-ILKTKEMYLARDNRRYGDMKDRITLSVKDLHKEAMGWF----EK 1089

Query: 1082 STHDENEEFAKASAWYHVTYHPNYWGRYNEGMQRDHFLSFPWCVSDKLIQIKREKKMCKI 1109
            S  DE ++   ASAWY+VTY+PN+        ++  FLSFPW V D L+ IK E    + 
Sbjct: 1090 SCEDEQQKKKLASAWYYVTYNPNHRD------EKLTFLSFPWIVGDVLLDIKAENAQRQS 1131

BLAST of Spg008341 vs. ExPASy Swiss-Prot
Match: Q7XM31 (Probable RNA-dependent RNA polymerase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=RDR2 PE=2 SV=1)

HSP 1 Score: 731.1 bits (1886), Expect = 1.9e-209
Identity = 433/1131 (38.28%), Postives = 641/1131 (56.68%), Query Frame = 0

Query: 2    ATIEIFGFPASVAADDVKEFLENHTG-DGTVSTVRISKPKDENSRFAFATVRFTSKLAAE 61
            A++ +   P S  A ++  F ++     G      I+            TV+F S  AA 
Sbjct: 10   ASLRVSNIPPSAVAAELLAFFDSAVAVAGGAFACEIAAAHRGWLSRGHGTVQFGSAAAAA 69

Query: 62   YVVAQAEAEERLWFESSYLKARGVEREMRAAAAEARRRGEVERIENVRIHLGNRISKERM 121
                 A +     F  + L       ++   A++   R          + +G+R+++   
Sbjct: 70   AAAGLASSGRLPRFLGALLSVSPSPVDLLPRASDLSLRA-----AGAGLVVGDRVAERVF 129

Query: 122  SVIWRGENCCVEFGVGIRKLCFYLSYEFAEYKMELSFENILGVELRCPLDRPSNLLLIQL 181
                  +    E   G R++  YL ++   YK+E+ FE++    L C LD     +L+QL
Sbjct: 130  EAADAWDGVRAEVIPGKRRVDLYLEHDSQRYKLEVLFEDMKDC-LGCTLD-GMGAILLQL 189

Query: 182  QGAPRIFKKSSSPSSSS--------SSKESTGSRWIRAVDFTPSSCIGQSFALCLEVSPG 241
              APRI    S P+ +S        + KE     W+RA+DFTP+   G+   L L++   
Sbjct: 190  NYAPRIHTAISGPAVNSRFMDDRFHACKEDAKFSWVRALDFTPNYSFGRCSTLVLKLGKS 249

Query: 242  DQLLSFFETLVGYKETYGPFNLETGSSFSSTSNLVPIISPPQGFDISYKILFKINALVQH 301
              +    ++L  +    G   + +     ++SN+VP++  P+ + + Y++LF++N+L+  
Sbjct: 250  ALVSDILKSL-PFSGNLGELTMNSMDGVGASSNVVPLVHCPRDYSVPYEVLFRLNSLMHM 309

Query: 302  GHLPGPLLDHEFFRLVDPSRFHPDFVEHALEKLSNLKECCYEPQKWLKQQYMSFYNSKQ- 361
            G +    ++ + F+ +       D      EK+  L+  CY P ++++Q+  S   S   
Sbjct: 310  GKIVAKHVNADLFKAL--QELPVDVSRRIFEKMHKLESTCYGPLQFIQQEAYSMKRSHNV 369

Query: 362  -LSWKPNISLEDGLVYVHRVQITPSKVYFRGPEANLSNRVVRRFIDDIDNFLRVSFVDEE 421
             LS +     E  L+  +RV ITPSK++  GPE  ++N VV+       +F+RV+FVDE+
Sbjct: 370  LLSNEGEGEGERKLMKCYRVNITPSKIFCFGPEEEVTNYVVKHHSAYASDFVRVTFVDED 429

Query: 422  LDKIRSIDLSPRTST---ENSERTSVYDRILYVLRNGIVIGEKKFEFLAFSASQLRENSF 481
              K+ S  +S R          +T +Y RIL +L+ G  IG K FEFLAFSASQLR NS 
Sbjct: 430  WSKLSSNAISARIEQGFFSKPFKTGLYYRILSILKKGFSIGPKNFEFLAFSASQLRGNSV 489

Query: 482  WMFASREGLTAADIREWMGDFRQIRNVAKYAARLGQSFGSSRKTLCVEEHEIEVIPDVEV 541
            WMFAS   L A  IR WMG F  IR+V+K AAR+GQ F SSR+T  V   ++EVIPD+E+
Sbjct: 490  WMFASNASLNAGGIRRWMGHFENIRSVSKCAARMGQLFSSSRQTFEVLRWDVEVIPDIEI 549

Query: 542  ERKKTAYCFSDGIGKISETLAEKVAEKCGLF-SHTPSAFQIRYAGYKGVVAIDPTSKKKL 601
                + Y FSDGIGKIS   A++VA   GL  ++ PSAFQIRY GYKGV+AIDP S   L
Sbjct: 550  TTDGSKYIFSDGIGKISLRFAKRVAHHVGLDPTNLPSAFQIRYGGYKGVIAIDPMSSIDL 609

Query: 602  SLRKSMLKYMSLDTQLDVLSWSRYQPCFLNRQVINLLSTLGIRDRVFVKKQKEAIDQLDA 661
            SLR SM K+ S    L++ SWS+ QPC++NR++I+LLSTLGIRD +FV  Q++ + + + 
Sbjct: 610  SLRPSMKKFESESRMLNITSWSKSQPCYVNREIISLLSTLGIRDEIFVAMQQDEMRETEE 669

Query: 662  ILKDPSKALEVLELISPGEMTSILKEMLLFYKPNEEPFLNMMLRTFRADKLLDLRTKSRI 721
            +L +   AL VL  +   E  + +K +L  Y+P+ EP+L+M+L+  + ++L D+RT+ +I
Sbjct: 670  MLTNKEVALSVLGKLGGSETKTAVKMLLQGYEPSSEPYLSMILKAHQENRLTDIRTRCKI 729

Query: 722  FVPKGRTMMGCLDETRTLEYGQVFVHCSVPSR----SSESNF--------VVTGKVVVAK 781
             VPKGR ++GCLDET  LEYGQV++  +  S+    S++S F         V GKV + K
Sbjct: 730  HVPKGRVLIGCLDETGVLEYGQVYIRITKNSKEQKDSNQSYFYNDDGKTATVVGKVAITK 789

Query: 782  NPCLHPGDVRLLDAVDVKALHHMVDCVVFPQKGERPHPNECSGSDLDGDLYFVCWDSELT 841
            NPCLHPGD+R+L+A+    L  MVDC+VFPQ+GERPHPNECSG DLDGDLYF+ WD +L 
Sbjct: 790  NPCLHPGDIRVLEAIYDPDLVGMVDCLVFPQRGERPHPNECSGGDLDGDLYFITWDDKLI 849

Query: 842  CIRPAKPMSYKPAPTMQLDHDVTIEEVQEYLTMYMVNDGPGGIANAHTVYADKKPKKAMS 901
              +   PM Y       +DH VT+EE+Q++   YM+ND  G I+ AH ++AD+ P KA S
Sbjct: 850  PEKVDTPMDYTATRPRIMDHVVTLEEIQKHFVDYMINDSLGAISTAHLIHADRSPLKARS 909

Query: 902  AECIKLAKLFSIAVDFPKTGVPANLPRSLRVQEYPDFMEKPDKPTYVSNGVLGKLFRRV- 961
             EC++LA L S+AVDF KTG PA +PR+LR +EYPDFME+ +KP Y+SNGVLGKL+R   
Sbjct: 910  PECLQLATLHSMAVDFAKTGAPAEMPRTLRPREYPDFMERWEKPMYISNGVLGKLYRSAM 969

Query: 962  --KDVSSDMNTIETFTKEVATECYDPDMEVDGFENYLREAFDCKNRYDFKLGNLMDYYGI 1021
               + S D   + + + +  +  YDPD+EV G + +L+ A +    Y+ KL  LM+YY  
Sbjct: 970  GHMEKSGDSGALSSSSAQ-PSPTYDPDLEVPGSDEFLQAAEEYYELYEEKLTTLMNYYRA 1029

Query: 1022 KTEPELVGGNILRVGKSFDKRND------LEQISLAMKSLRKEARAWFNEKGSKSTHDEN 1081
            + E E++ GNI R    + KR++       ++I  A+ +L +EAR W       S+  E 
Sbjct: 1030 ELEDEILTGNI-RNKMLYLKRDNKRYFEMKDRIVAAVDALHREARGWL-----LSSRKEE 1089

Query: 1082 EEFAKASAWYHVTYHPNYWGRYNEGMQRDHFLSFPWCVSDKLIQIKREKKM 1097
            +    ASAWY VTYHP       +  +   F SFPW   D L+ IK   ++
Sbjct: 1090 DASRMASAWYRVTYHP-------DRRRGKRFWSFPWIACDNLLAIKASSQL 1116

BLAST of Spg008341 vs. ExPASy Swiss-Prot
Match: Q9SG02 (RNA-dependent RNA polymerase 6 OS=Arabidopsis thaliana OX=3702 GN=RDR6 PE=1 SV=1)

HSP 1 Score: 577.0 bits (1486), Expect = 4.6e-163
Identity = 406/1089 (37.28%), Postives = 579/1089 (53.17%), Query Frame = 0

Query: 96   RRRGEVE-RIENVRIHLGNRISKERMSVIWRGENCCVEFGV----GIRKLCFYLSYEFA- 155
            RRR  V  ++  + + +G  +S++   V WR E   V+F V       K CF  S  F+ 
Sbjct: 123  RRRTTVPYKLAGITLEIGTLVSRDDFFVSWRAEG--VDFLVDPFDNTCKFCFRKSTAFSF 182

Query: 156  -----------EYKMELSFENILGVELRCPLDRPSNLLLIQLQGAPRIFKKSSSP---SS 215
                       +YK+EL   +I  V     L     +L++QL  +PR++ +++      +
Sbjct: 183  KDAVMHAVINCDYKLELLVRDIQTVRQYKTLH--GFVLILQLASSPRVWYRTADDDIYDT 242

Query: 216  SSSSKESTGSRWIRAVDFTPSSCIGQSFALCLEVSPGDQ-----LLSFFE-TLVGYKETY 275
                       WIR  DFT    IG+  +  + +SP  +      L +F    V  +   
Sbjct: 243  VPGDLLDDDDPWIRTTDFTQVGAIGRCHSYRVLISPRYENKLRTALDYFRMRRVQEERVR 302

Query: 276  GPFNLETGSSFSS-TSNLVPIISPPQGFDISYKILFKINALVQHGHLPGPLLDHEFFRLV 335
             P  +     F    S+    I   +G  IS++I+F +N+++  G      L   FF L+
Sbjct: 303  WPPRIRNEPCFGEPVSDHFFCIHHKEG--ISFEIMFLVNSVLHRGVFNQFQLTERFFDLL 362

Query: 336  DPSRFHPDFVEHA-LEKLSNLKECCYEPQKWLK--QQYMSFYNSKQLSWKPNISLEDGLV 395
               R  P  V  A L+ L   K   ++  K LK  Q+++   N K L    +    + + 
Sbjct: 363  ---RNQPKDVNIASLKHLCTYKRPVFDAYKRLKLVQEWIQ-KNPKLLG---SHEQSEDIS 422

Query: 396  YVHRVQITPSKVYFRGPEANLSNRVVRRFIDDIDNFLRVSFVDEELDKIRSIDLS----- 455
             + R+ ITP++ Y   PE  LSNRV+RR+    + FLRV+F+DE +  I S  LS     
Sbjct: 423  EIRRLVITPTRAYCLPPEVELSNRVLRRYKAVAERFLRVTFMDESMQTINSNVLSYFVAP 482

Query: 456  ---PRTSTENSERTSVYDRILYVLRNGIVIGEKKFEFLAFSASQLRENSFWMFASREGLT 515
                 TS+  S++T V+ R+  +L +G  +  +K+ FLAFSA+QLR+ S W FA      
Sbjct: 483  IVKDLTSSSFSQKTYVFKRVKSILTDGFKLCGRKYSFLAFSANQLRDRSAWFFAEDGKTR 542

Query: 516  AADIREWMGDFRQIRNVAKYAARLGQSFGSSRKTLCVEEHEIEV-IPDVEVERKKTAYCF 575
             +DI+ WMG F+  +NVAK AAR+G  F S+  T+ V  HE++  +PD+E    +  Y F
Sbjct: 543  VSDIKTWMGKFKD-KNVAKCAARMGLCFSSTYATVDVMPHEVDTEVPDIE----RNGYVF 602

Query: 576  SDGIGKISETLAEKVAEKCGLFSH-TPSAFQIRYAGYKGVVAIDPTSKK--KLSLRKSML 635
            SDGIG I+  LA++V EK  L  H +P A+QIRYAG+KGVVA  P+     +L+LR SM 
Sbjct: 603  SDGIGTITPDLADEVMEKLKLDVHYSPCAYQIRYAGFKGVVARWPSKSDGIRLALRDSMK 662

Query: 636  KYMSLDTQLDVLSWSRYQPCFLNRQVINLLSTLGIRDRVFVKKQKEAIDQLDAILKDPSK 695
            K+ S  T L++ SW+R+QP FLNRQ+I LLS LG+ D +F   Q+  + +L+ IL D   
Sbjct: 663  KFFSKHTILEICSWTRFQPGFLNRQIITLLSVLGVPDEIFWDMQESMLYKLNRILDDTDV 722

Query: 696  ALEVLELISPGEMTSILKEMLLFYKPNEEPFLNMMLRTFRADKLLDLRTKSRIFVPKGRT 755
            A EVL      +  +    +   +KP  EP L  ML + R  +L  LR KSRIFV  GR 
Sbjct: 723  AFEVLTASCAEQGNTAAIMLSAGFKPKTEPHLRGMLSSVRIAQLWGLREKSRIFVTSGRW 782

Query: 756  MMGCLDETRTLEYGQVFVHCSVP----------SRSSESNF---VVTGKVVVAKNPCLHP 815
            +MGCLDE   LE+GQ F+  S P          SR  E+     VV G V +AKNPCLHP
Sbjct: 783  LMGCLDEAGILEHGQCFIQVSKPSIENCFSKHGSRFKETKTDLEVVKGYVAIAKNPCLHP 842

Query: 816  GDVRLLDAVDVKALHHMVDCVVFPQKGERPHPNECSGSDLDGDLYFVCWDSELTCIRPAK 875
            GDVR+L+AVDV  LHHM DC++FPQKG+RPH NE SGSDLDGDLYFV WD +L  I P +
Sbjct: 843  GDVRILEAVDVPQLHHMYDCLIFPQKGDRPHTNEASGSDLDGDLYFVAWDQKL--IPPNR 902

Query: 876  ----PMSYKPAPTMQLDHDVTIEEVQEYLTMYMVNDGPGGIANAHTVYADKKPKKAMSAE 935
                 M Y  A    L   V  +++ ++    + N+  G I NAH V+AD+    AM  E
Sbjct: 903  KSYPAMHYDAAEEKSLGRAVNHQDIIDFFARNLANEQLGTICNAHVVHADRSEYGAMDEE 962

Query: 936  CIKLAKLFSIAVDFPKTGVPANLPRSLRVQEYPDFMEKPDKPTYVSNGVLGKLFRRVKDV 995
            C+ LA+L + AVDFPKTG   ++P  L+ + YPDFM K D  TY SN +LG+L+RRVK+V
Sbjct: 963  CLLLAELAATAVDFPKTGKIVSMPFHLKPKLYPDFMGKEDYQTYKSNKILGRLYRRVKEV 1022

Query: 996  -SSDMNTIETFTKEVATECYDPDMEVDGFENYLREAFDCKNRYDFKLGNLMDYYGIKTEP 1055
               D       + + +   YD  +E+ GFE+ + EA+  K  YD +L  L+  Y ++ E 
Sbjct: 1023 YDEDAEASSEESTDPSAIPYDAVLEIPGFEDLIPEAWGHKCLYDGQLIGLLGQYKVQKEE 1082

Query: 1056 ELVGGNILRVGKSFDKRND--LEQISLAMKSLRKEARAWFNEKGSKSTHDENEE-----F 1112
            E+V G+I  + K   K+     E++  +  SL+KE R  F E      +   EE      
Sbjct: 1083 EIVTGHIWSMPKYTSKKQGELKERLKHSYNSLKKEFRKVFEETIPDHENLSEEEKNILYE 1142

BLAST of Spg008341 vs. ExPASy TrEMBL
Match: A0A6J1FF44 (RNA-dependent RNA polymerase OS=Cucurbita moschata OX=3662 GN=LOC111444909 PE=3 SV=1)

HSP 1 Score: 1855.9 bits (4806), Expect = 0.0e+00
Identity = 923/1107 (83.38%), Postives = 1000/1107 (90.33%), Query Frame = 0

Query: 3    TIEIFGFPASVAADDVKEFLENHTGDGTVSTVRISKPKDENSRFAFATVRFTSKLAAEYV 62
            TIEI+GFP  V AD+VKEF+ENHTGDGTV TVRISK  DE +RF FATV+FTSKL  EYV
Sbjct: 10   TIEIYGFPTQVTADEVKEFIENHTGDGTVLTVRISKLNDEKARFTFATVQFTSKLGGEYV 69

Query: 63   VAQAEAEERLWFESSYLKARGVEREMRAAAAEARRRGEVERIENVRIHLGNRISKERMSV 122
            VAQA AE+RLWF SSYLKAR VERE++ A   AR  GE+ER+ENV +  G+ +SKE M V
Sbjct: 70   VAQAAAEKRLWFGSSYLKARKVEREIKTA---ARVHGELERMENVNVQWGSLVSKEEMKV 129

Query: 123  IWRGENCCVEFGVGIRKLCFYLSYEFAEYKMELSFENILGVELRCPLDRPSNLLLIQLQG 182
            IW+G    VE+G+G+RKL FYLSYE  EYKMEL FENILGV+LRC +DR S L LIQLQG
Sbjct: 130  IWKGGKWSVEYGIGVRKLGFYLSYEGVEYKMELCFENILGVQLRCSVDRGSKLFLIQLQG 189

Query: 183  APRIFKKSS-SPSSSSSSKESTGSRWIRAVDFTPSSCIGQSFALCLEVSPGDQLLSFFET 242
            APRIFKK+  S SS  SS+ESTG RWIR VDFTPSSCIGQSFALCLE+S GDQL SFF+T
Sbjct: 190  APRIFKKAPLSSSSRLSSEESTGFRWIRDVDFTPSSCIGQSFALCLELSHGDQLPSFFQT 249

Query: 243  LVGYKETYGPFNLETGSSFSSTSNLVPIISPPQGFDISYKILFKINALVQHGHLPGPLLD 302
            LVGYKE+Y PF L+TGSS SS SNLVPII+PPQGFDI YKILFKINAL+QHG+LPGP LD
Sbjct: 250  LVGYKESYAPFILQTGSSLSSISNLVPIITPPQGFDIPYKILFKINALLQHGYLPGPALD 309

Query: 303  HEFFRLVDPSRFHPDFVEHALEKLSNLKECCYEPQKWLKQQYMSFYNSKQLSWKPNISLE 362
            +EFFRLVD SRF PD+VE+AL+KL + KECCYEPQKWLKQQY+SFY+SKQL WKPNISL+
Sbjct: 310  NEFFRLVDSSRFRPDYVEYALDKLFHFKECCYEPQKWLKQQYLSFYSSKQLPWKPNISLD 369

Query: 363  DGLVYVHRVQITPSKVYFRGPEANLSNRVVRRFIDDIDNFLRVSFVDEELDKIRSIDLSP 422
            DGLVYVHRVQITP+KVYFRGPEANLSNRVVR FIDD+DNFLRVSFVDEELDK+ SIDLSP
Sbjct: 370  DGLVYVHRVQITPTKVYFRGPEANLSNRVVRHFIDDVDNFLRVSFVDEELDKLHSIDLSP 429

Query: 423  RTSTENSERTSVYDRILYVLRNGIVIGEKKFEFLAFSASQLRENSFWMFASREGLTAADI 482
            RTS EN  RT VYDR+L VLRNGIVIG+KKFEFLAFSASQLRENS WMFASR+GL+AADI
Sbjct: 430  RTSAENDLRTRVYDRVLSVLRNGIVIGDKKFEFLAFSASQLRENSCWMFASRKGLSAADI 489

Query: 483  REWMGDFRQIRNVAKYAARLGQSFGSSRKTLCVEEHEIEVIPDVEVERKKTAYCFSDGIG 542
            REWMGD RQIRNVAKYAARLGQSFGSSR+TLCVE+HEIEVI DVEVE  K  YCFSDGIG
Sbjct: 490  REWMGDLRQIRNVAKYAARLGQSFGSSRRTLCVEQHEIEVISDVEVETNKITYCFSDGIG 549

Query: 543  KISETLAEKVAEKCGLFSHTPSAFQIRYAGYKGVVAIDPTSKKKLSLRKSMLKYMSLDTQ 602
            KIS TLAEKVAEKCGL SHTPSAFQIRYAGYKGVVAIDPTS KKLSLRKSMLKY SLDTQ
Sbjct: 550  KISGTLAEKVAEKCGLISHTPSAFQIRYAGYKGVVAIDPTSTKKLSLRKSMLKYTSLDTQ 609

Query: 603  LDVLSWSRYQPCFLNRQVINLLSTLGIRDRVFVKKQKEAIDQLDAILKDPSKALEVLELI 662
            +DVLSWS+YQPCFLNRQVINLLSTLGI+DRVFVKKQKEAIDQLD+IL+DPS+ALEVLEL+
Sbjct: 610  VDVLSWSKYQPCFLNRQVINLLSTLGIKDRVFVKKQKEAIDQLDSILEDPSRALEVLELM 669

Query: 663  SPGEMTSILKEMLLFYKPNEEPFLNMMLRTFRADKLLDLRTKSRIFVPKGRTMMGCLDET 722
            SPGEMTSILKE+LL YKPNEEPFLNMMLRTFRA KLLDLRTKSRIFVPKGRTMMGCLDET
Sbjct: 670  SPGEMTSILKELLLLYKPNEEPFLNMMLRTFRAAKLLDLRTKSRIFVPKGRTMMGCLDET 729

Query: 723  RTLEYGQVFVHCSVPSRSSESNFVVTGKVVVAKNPCLHPGDVRLLDAVDVKALHHMVDCV 782
            RTLEYGQVFVHCS+P RSSESNFVV GKVVVAKNPCLHPGDVRLLDAVDVKALHHMVDCV
Sbjct: 730  RTLEYGQVFVHCSLPGRSSESNFVVKGKVVVAKNPCLHPGDVRLLDAVDVKALHHMVDCV 789

Query: 783  VFPQKGERPHPNECSGSDLDGDLYFVCWDSELTCIRPAKPMSYKPAPTMQLDHDVTIEEV 842
            VFPQKG+RPHPNECSGSDLDGDLYFV WD ELT I+P KPMSY+PAPTMQLDHDVTIEEV
Sbjct: 790  VFPQKGKRPHPNECSGSDLDGDLYFVGWDPELTRIKPVKPMSYEPAPTMQLDHDVTIEEV 849

Query: 843  QEYLTMYMVNDGPGGIANAHTVYADKKPKKAMSAECIKLAKLFSIAVDFPKTGVPANLPR 902
            Q YL  YMVNDG GGIANAHTV+ADKKPKKAM+AECIKLAKLFSIAVDFPKTGVPAN P 
Sbjct: 850  QGYLVKYMVNDGLGGIANAHTVFADKKPKKAMAAECIKLAKLFSIAVDFPKTGVPANFPH 909

Query: 903  SLRVQEYPDFMEKPDKPTYVSNGVLGKLFRRVKDVSSDMNTIETFTKEVATECYDPDMEV 962
            +LRV+EYPDFMEKPDKPTYVSNGVLGKLFR VKDVSSD+NT+E F++EVAT+CYDPDMEV
Sbjct: 910  NLRVREYPDFMEKPDKPTYVSNGVLGKLFRGVKDVSSDVNTVEIFSREVATKCYDPDMEV 969

Query: 963  DGFENYLREAFDCKNRYDFKLGNLMDYYGIKTEPELVGGNILRVGKSFDKRNDLEQISLA 1022
            DGFE+YL EAF+ K RYDFKLGNLMDYYGIKTEPEL+ GNILR+ KSFDKRND+EQISLA
Sbjct: 970  DGFEDYLSEAFEFKARYDFKLGNLMDYYGIKTEPELISGNILRMAKSFDKRNDMEQISLA 1029

Query: 1023 MKSLRKEARAWFNEKGSKSTHDE----NEEFAKASAWYHVTYHPNYWGRYNEGMQRDHFL 1082
            MKSLRKEAR+WFNEKGSKS +DE    NEE+AKASAWY VTYHP+YWGRYNEGMQRDHFL
Sbjct: 1030 MKSLRKEARSWFNEKGSKSAYDEDDNKNEEYAKASAWYRVTYHPDYWGRYNEGMQRDHFL 1089

Query: 1083 SFPWCVSDKLIQIKREKKMCKISSSQV 1105
            SFPWCVSDKLIQIKRE K C ++ S V
Sbjct: 1090 SFPWCVSDKLIQIKRE-KTCLVNFSPV 1112

BLAST of Spg008341 vs. ExPASy TrEMBL
Match: A0A6J1K1Q3 (RNA-dependent RNA polymerase OS=Cucurbita maxima OX=3661 GN=LOC111489826 PE=3 SV=1)

HSP 1 Score: 1855.5 bits (4805), Expect = 0.0e+00
Identity = 926/1107 (83.65%), Postives = 1001/1107 (90.42%), Query Frame = 0

Query: 4    IEIFGFPASVAADDVKEFLENHTGDGTVSTVRISKPKDENSRFAFATVRFTSKLAAEYVV 63
            IEI+GFPA V AD+VKEF+ENHTGDGTV TVRISKP DE +RF FATVRFTSKL  EYVV
Sbjct: 11   IEIYGFPAEVTADEVKEFIENHTGDGTVLTVRISKPNDEKARFTFATVRFTSKLGGEYVV 70

Query: 64   AQAEAEERLWFESSYLKARGVEREMR--AAAAEARRRGEVERIENVRIHLGNRISKERMS 123
            AQA AE+RLWF SSYLKAR VERE+R   AAA AR  G +ER+ENV +  G+ +SKE+M 
Sbjct: 71   AQAAAEKRLWFGSSYLKARKVEREIRTATAAAAARVHGGLERMENVNVQWGSLVSKEKMK 130

Query: 124  VIWRGENCCVEFGVGIRKLCFYLSYEFAEYKMELSFENILGVELRCPLDRPSNLLLIQLQ 183
            VIW+     VE+G+G RKL FYLSYE  EYKMEL FENILGV+LRC  DR S L LIQLQ
Sbjct: 131  VIWKEGKWSVEYGIGARKLGFYLSYEGVEYKMELCFENILGVQLRCSGDRGSELFLIQLQ 190

Query: 184  GAPRIFKKSSSPSSSS-SSKESTGSRWIRAVDFTPSSCIGQSFALCLEVSPGDQLLSFFE 243
            GAPRIFKK+ S SSS  SS+ESTG RWIR VDFTPSSCIGQSFALCLE+SPGDQL SFF 
Sbjct: 191  GAPRIFKKTPSSSSSRLSSEESTGFRWIRDVDFTPSSCIGQSFALCLELSPGDQLPSFFR 250

Query: 244  TLVGYKETYGPFNLETGSSFSSTSNLVPIISPPQGFDISYKILFKINALVQHGHLPGPLL 303
            TLVGYKE+Y PF L  GSS SS SNLVPII+PPQGFDISYKILFKINAL+QHG+LPGP L
Sbjct: 251  TLVGYKESYAPFILHPGSSLSSISNLVPIITPPQGFDISYKILFKINALLQHGYLPGPAL 310

Query: 304  DHEFFRLVDPSRFHPDFVEHALEKLSNLKECCYEPQKWLKQQYMSFYNSKQLSWKPNISL 363
            D+EFFRLVD SRF P+++E+ALEKL + KECCYEPQKWLKQQY+SFY+SKQL  KPN+SL
Sbjct: 311  DNEFFRLVDSSRFRPEYIEYALEKLFHFKECCYEPQKWLKQQYLSFYSSKQLPCKPNVSL 370

Query: 364  EDGLVYVHRVQITPSKVYFRGPEANLSNRVVRRFIDDIDNFLRVSFVDEELDKIRSIDLS 423
            +DGLVYVHRVQITP+KVYFRGPEANLSNRVVR FIDD+DNFLRVSFVDEELDK+ SIDLS
Sbjct: 371  DDGLVYVHRVQITPTKVYFRGPEANLSNRVVRHFIDDVDNFLRVSFVDEELDKLHSIDLS 430

Query: 424  PRTSTENSERTSVYDRILYVLRNGIVIGEKKFEFLAFSASQLRENSFWMFASREGLTAAD 483
            PRTS EN  RT VYDR+L VLRNGIVIG+KKFEFLAFSASQLRENS WMFASR+GL+AAD
Sbjct: 431  PRTSAENDLRTRVYDRVLSVLRNGIVIGDKKFEFLAFSASQLRENSCWMFASRKGLSAAD 490

Query: 484  IREWMGDFRQIRNVAKYAARLGQSFGSSRKTLCVEEHEIEVIPDVEVERKKTAYCFSDGI 543
            IREWMGDFRQIRNVAKYAARLGQSFGSSR+TLCVE+HEIEVIPDVEVE  K  YCFSDGI
Sbjct: 491  IREWMGDFRQIRNVAKYAARLGQSFGSSRRTLCVEQHEIEVIPDVEVETNKITYCFSDGI 550

Query: 544  GKISETLAEKVAEKCGLFSHTPSAFQIRYAGYKGVVAIDPTSKKKLSLRKSMLKYMSLDT 603
            GKIS TLAEKVAEKCGL SHTPSAFQIRYAGYKGVVAIDPTS KKLSLRKSMLKY SLDT
Sbjct: 551  GKISGTLAEKVAEKCGLISHTPSAFQIRYAGYKGVVAIDPTSTKKLSLRKSMLKYTSLDT 610

Query: 604  QLDVLSWSRYQPCFLNRQVINLLSTLGIRDRVFVKKQKEAIDQLDAILKDPSKALEVLEL 663
            Q+DVLSWS+YQPCFLNRQVINLLSTLGI+DRVFVKKQKEAIDQLD+IL+DPS+ALEVLEL
Sbjct: 611  QVDVLSWSKYQPCFLNRQVINLLSTLGIKDRVFVKKQKEAIDQLDSILEDPSRALEVLEL 670

Query: 664  ISPGEMTSILKEMLLFYKPNEEPFLNMMLRTFRADKLLDLRTKSRIFVPKGRTMMGCLDE 723
            +SPGEMTSILKE+LL YKPNEEPFLNMMLRTFRA KLLDLRTKSRIFVPKGRTMMGCLDE
Sbjct: 671  MSPGEMTSILKELLLLYKPNEEPFLNMMLRTFRAAKLLDLRTKSRIFVPKGRTMMGCLDE 730

Query: 724  TRTLEYGQVFVHCSVPSRSSESNFVVTGKVVVAKNPCLHPGDVRLLDAVDVKALHHMVDC 783
            TRTLEYGQVFVHCS+P RSSESNFVV GKVVVAKNPCLHPGDVRLLDAVDVKALHHMV+C
Sbjct: 731  TRTLEYGQVFVHCSLPGRSSESNFVVKGKVVVAKNPCLHPGDVRLLDAVDVKALHHMVNC 790

Query: 784  VVFPQKGERPHPNECSGSDLDGDLYFVCWDSELTCIRPAKPMSYKPAPTMQLDHDVTIEE 843
            VVFPQKGERPHPNECSGSDLDGDLYFV WD ELT I+P KPMSY+PAPTM+LDHDVTIEE
Sbjct: 791  VVFPQKGERPHPNECSGSDLDGDLYFVGWDPELTRIKPVKPMSYEPAPTMRLDHDVTIEE 850

Query: 844  VQEYLTMYMVNDGPGGIANAHTVYADKKPKKAMSAECIKLAKLFSIAVDFPKTGVPANLP 903
            VQEYL  YMVNDG GGIANAHTV+ADKKPKKAM+AECIKLAKLFSIAVDFPKTGVPAN P
Sbjct: 851  VQEYLAKYMVNDGLGGIANAHTVFADKKPKKAMTAECIKLAKLFSIAVDFPKTGVPANFP 910

Query: 904  RSLRVQEYPDFMEKPDKPTYVSNGVLGKLFRRVKDVSSDMNTIETFTKEVATECYDPDME 963
             +LRV+EYPDFMEKPDKPTYVSNGVLGKLFR VKDVSSD+NTIE F++EVAT+CYDPDME
Sbjct: 911  HNLRVREYPDFMEKPDKPTYVSNGVLGKLFRGVKDVSSDVNTIEIFSREVATKCYDPDME 970

Query: 964  VDGFENYLREAFDCKNRYDFKLGNLMDYYGIKTEPELVGGNILRVGKSFDKRNDLEQISL 1023
            VDGFE+YL EAF+ K RYDFKLGNLMDYYGIKTEPEL+ GNILR+ KSFDKRNDLEQISL
Sbjct: 971  VDGFEDYLSEAFEYKARYDFKLGNLMDYYGIKTEPELISGNILRMAKSFDKRNDLEQISL 1030

Query: 1024 AMKSLRKEARAWFNEKGSKSTHDE---NEEFAKASAWYHVTYHPNYWGRYNEGMQRDHFL 1083
            A+KSLRKEAR+WFNEKGSKS  DE   +EE+AKASAWY VTYHP+YWGRYNEGMQRDHFL
Sbjct: 1031 AIKSLRKEARSWFNEKGSKSACDEDDKDEEYAKASAWYRVTYHPDYWGRYNEGMQRDHFL 1090

Query: 1084 SFPWCVSDKLIQIKREKKMCKISSSQV 1105
            SFPWCVSDKLIQIKRE K C ++ S +
Sbjct: 1091 SFPWCVSDKLIQIKRE-KTCLVNLSPI 1116

BLAST of Spg008341 vs. ExPASy TrEMBL
Match: A0A6J1JTB1 (RNA-dependent RNA polymerase OS=Cucurbita maxima OX=3661 GN=LOC111487656 PE=3 SV=1)

HSP 1 Score: 1854.7 bits (4803), Expect = 0.0e+00
Identity = 930/1124 (82.74%), Postives = 997/1124 (88.70%), Query Frame = 0

Query: 3    TIEIFGFPASVAADDVKEFLENHTGDGTVSTVRISKPKDENSRFAFATVRFTSKLAAEYV 62
            TIE+FGF   V+A +VKEF+ENHTG+GTVS VRISKPKD   R  FA V F SK+AAEYV
Sbjct: 7    TIEVFGFRREVSAGEVKEFMENHTGEGTVSKVRISKPKDGKGRLTFARVEFRSKVAAEYV 66

Query: 63   VAQAEAEERLWFESSYLKARGVEREMRAAAAEARRRGEVERIENVRIHLGNRISKERMSV 122
            V +AEAEE LWF   YLKARGVEREMR           VER+E VR+ LG+ ISKE M V
Sbjct: 67   VEKAEAEEGLWFGRWYLKARGVEREMR-----------VERMEKVRVQLGSVISKEEMRV 126

Query: 123  IWRGENCCVEFGVGIRKLCFYLSY--EFAEYKMELSFENILGVELRCPLDRPSNLLLIQL 182
            IWRGE   VE+G G+RKL FYLSY  E  +YKMEL FENILGV+LRCP +  SNL LIQL
Sbjct: 127  IWRGEEWSVEYGNGMRKLWFYLSYGVEDDDYKMELRFENILGVQLRCPHNSSSNLFLIQL 186

Query: 183  QGAPRIFKKSSSPSSSSS---SKESTGSRWIRAVDFTPSSCIGQSFALCLEVSPGDQLLS 242
            QGAPRIFKK  S SSSSS   SKESTG RWIR VDFTPSSCIGQSF+LCLEVSPGDQL S
Sbjct: 187  QGAPRIFKKCPSSSSSSSQFYSKESTGFRWIRDVDFTPSSCIGQSFSLCLEVSPGDQLPS 246

Query: 243  FFETLVGYKETYGPFNLETGSSFSSTSNLVPIISPPQGFDISYKILFKINALVQHGHLPG 302
            FF+TLVGYKE YGPF L TGS+FSSTSNLVPI++PPQ FDI YKILFKINALVQHG+LPG
Sbjct: 247  FFQTLVGYKEIYGPFKLSTGSAFSSTSNLVPIVTPPQPFDIPYKILFKINALVQHGYLPG 306

Query: 303  PLLDHEFFRLVDPSRFHPDFVEHALEKLSNLKECCYEPQKWLKQQYMSFYNSKQLSWKPN 362
              LD +FFRLVDPSRF  D++E+ALEKL +LKECCYEPQKWLKQQY+SF +S QL WK N
Sbjct: 307  SALDDKFFRLVDPSRFRSDYIEYALEKLFSLKECCYEPQKWLKQQYLSFCSSMQLPWKAN 366

Query: 363  ISLEDGLVYVHRVQITPSKVYFRGPEANLSNRVVRRFIDDIDNFLRVSFVDEELDKIRSI 422
            ISL+D LVYVHRVQITP+KVYFRGPEANLSNRVVRRFIDDIDNFLRVSFVDEELDKI S 
Sbjct: 367  ISLDDSLVYVHRVQITPAKVYFRGPEANLSNRVVRRFIDDIDNFLRVSFVDEELDKIHSS 426

Query: 423  DLSPRTSTENSERTSVYDRILYVLRNGIVIGEKKFEFLAFSASQLRENSFWMFASREGLT 482
            DLSPRTSTE  ERT VYDRIL VLRNGIVIG KKFEFLAFSASQLRENSFWMFASREGL 
Sbjct: 427  DLSPRTSTEKIERTRVYDRILSVLRNGIVIGGKKFEFLAFSASQLRENSFWMFASREGLN 486

Query: 483  AADIREWMGDFRQIRNVAKYAARLGQSFGSSRKTLCVEEHEIEVIPDVEVERKKTAYCFS 542
            AADIREWMGDFR+IRNVAKYAARLGQSFGSSRKTLCVEE+EIEVIPD EVERK   YCFS
Sbjct: 487  AADIREWMGDFREIRNVAKYAARLGQSFGSSRKTLCVEENEIEVIPDAEVERKNIKYCFS 546

Query: 543  DGIGKISETLAEKVAEKCGLFSHTPSAFQIRYAGYKGVVAIDPTSKKKLSLRKSMLKYMS 602
            DGIGKISE LAE VAEKCGL SHTPSAFQIRYAGYKGVVA+DPTSKKKLSLRKSMLKYMS
Sbjct: 547  DGIGKISEALAEMVAEKCGLISHTPSAFQIRYAGYKGVVAVDPTSKKKLSLRKSMLKYMS 606

Query: 603  LDTQLDVLSWSRYQPCFLNRQVINLLSTLGIRDRVFVKKQKEAIDQLDAILKDPSKALEV 662
            LDTQLDVLSWS+YQPCFLNRQVINLLSTLGIRD VFVK+QK+A+DQLD+IL+DPSKALEV
Sbjct: 607  LDTQLDVLSWSKYQPCFLNRQVINLLSTLGIRDDVFVKQQKKAVDQLDSILRDPSKALEV 666

Query: 663  LELISPGEMTSILKEMLLFYKPNEEPFLNMMLRTFRADKLLDLRTKSRIFVPKGRTMMGC 722
            LEL+SPGEMTS+LK+MLLFY P+EEPFLNMMLRTFRADKLLDLRTKSRIFVPKGRTMMGC
Sbjct: 667  LELMSPGEMTSVLKDMLLFYNPHEEPFLNMMLRTFRADKLLDLRTKSRIFVPKGRTMMGC 726

Query: 723  LDETRTLEYGQVFVHCSVPSRSSESNFVVTGKVVVAKNPCLHPGDVRLLDAVDVKALHHM 782
            LDETRTLEYGQVF+HCSVP RSSESNFVV GKVVVAKNPCLHPGDVR+LDAVDVK LHHM
Sbjct: 727  LDETRTLEYGQVFIHCSVPGRSSESNFVVKGKVVVAKNPCLHPGDVRVLDAVDVKTLHHM 786

Query: 783  VDCVVFPQKGERPHPNECSGSDLDGDLYFVCWDSELTCIRPAKPMSYKPAPTMQLDHDVT 842
            VDCVVFPQKG+RPHPNECSGSDLDGDLYFVCWD ELTCIRP KPMSY+PAPT+QLDHDVT
Sbjct: 787  VDCVVFPQKGKRPHPNECSGSDLDGDLYFVCWDPELTCIRPIKPMSYEPAPTIQLDHDVT 846

Query: 843  IEEVQEYLTMYMVNDGPGGIANAHTVYADKKPKKAMSAECIKLAKLFSIAVDFPKTGVPA 902
            +EEVQEY   YMVNDG GGIANAHTV+AD KPKKAMSAECI+LAKLFSIAVDFPKTGVPA
Sbjct: 847  LEEVQEYFANYMVNDGLGGIANAHTVFADMKPKKAMSAECIELAKLFSIAVDFPKTGVPA 906

Query: 903  NLPRSLRVQEYPDFMEKPDKPTYVSNGVLGKLFRRVKDVSSDMNTIETFTKEVATECYDP 962
            NLPRSLRV EYPDFMEKP+K TY+SNGV+GKLFR VKDVSSD+N IETFT+EVA +CYDP
Sbjct: 907  NLPRSLRVHEYPDFMEKPNKRTYISNGVIGKLFRGVKDVSSDINNIETFTREVAVKCYDP 966

Query: 963  DMEVDGFENYLREAFDCKNRYDFKLGNLMDYYGIKTEPELVGGNILRVGKSFDKRNDLEQ 1022
            DMEV GFENYL EAFD K  YDFKLGNLMDYYGI+TEPELV GNILR+GKSFDKRND+EQ
Sbjct: 967  DMEVMGFENYLSEAFDYKRMYDFKLGNLMDYYGIETEPELVSGNILRMGKSFDKRNDMEQ 1026

Query: 1023 ISLAMKSLRKEARAWFNEKGSKSTHDENE----EFAKASAWYHVTYHPNYWGRYNEGMQR 1082
            ISLAM+SLRKEAR WFNEK SKS ++ N+    E+AKASAWYHVTYHPN+WGRYNEGMQR
Sbjct: 1027 ISLAMRSLRKEARGWFNEKASKSIYNNNKGEDNEYAKASAWYHVTYHPNFWGRYNEGMQR 1086

Query: 1083 DHFLSFPWCVSDKLIQIKREKKMCKISSSQVSSLIHKFGNLSLY 1118
            DHFLSFPWCVSDKLIQIKRE    K+ SS VSSLIHKFG LS Y
Sbjct: 1087 DHFLSFPWCVSDKLIQIKRE----KMESSPVSSLIHKFGKLSFY 1115

BLAST of Spg008341 vs. ExPASy TrEMBL
Match: A0A6J1EB47 (RNA-dependent RNA polymerase OS=Cucurbita moschata OX=3662 GN=LOC111430971 PE=3 SV=1)

HSP 1 Score: 1842.0 bits (4770), Expect = 0.0e+00
Identity = 926/1125 (82.31%), Postives = 998/1125 (88.71%), Query Frame = 0

Query: 3    TIEIFGFPASVAADDVKEFLENHTGDGTVSTVRISKPKDENSRFAFATVRFTSKLAAEYV 62
            TIE+FGF   V+A +VKEF+ENHTG+GTVS VRISKPK E SR   A V F SK+AAEYV
Sbjct: 7    TIEVFGFGREVSAGEVKEFMENHTGEGTVSKVRISKPKYEKSRLTSARVEFRSKVAAEYV 66

Query: 63   VAQAEAEERLWFESSYLKARGVEREMRAAAAEARRRGEVERIENVRIHLGNRISKERMSV 122
            V +AEAEERLWF   YLKAR VERE         R   +ER+E VR+ LG+ I K+ M V
Sbjct: 67   VEKAEAEERLWFGRWYLKARRVERERE----REMRVERLERLEKVRVQLGSVIWKDEMRV 126

Query: 123  IWRGENCCVEFGVGIRKLCFYLSY--EFAEYKMELSFENILGVELRCPLDRPSNLLLIQL 182
            IWRGE+  VE+G G+RKL FYLSY  E  +YKMEL FENILGV+LRCP +  SNL LIQL
Sbjct: 127  IWRGEDWSVEYGNGMRKLWFYLSYGVEDDDYKMELRFENILGVQLRCPHNSSSNLFLIQL 186

Query: 183  QGAPRIFKKSSSPSSSSS----SKESTGSRWIRAVDFTPSSCIGQSFALCLEVSPGDQLL 242
            QGAPRIFKK  S SSSSS    SKESTG RWIR VDFTPSSCIGQSFALC EVSPGDQL 
Sbjct: 187  QGAPRIFKKCPSSSSSSSSQFYSKESTGFRWIRDVDFTPSSCIGQSFALCFEVSPGDQLP 246

Query: 243  SFFETLVGYKETYGPFNLETGSSFSSTSNLVPIISPPQGFDISYKILFKINALVQHGHLP 302
            SFF+TLVGYKE YGPF L TGS+FSSTSNLVPI++PPQ F I YKILFKINALVQHG+LP
Sbjct: 247  SFFQTLVGYKEIYGPFKLSTGSAFSSTSNLVPIVTPPQPFHIPYKILFKINALVQHGYLP 306

Query: 303  GPLLDHEFFRLVDPSRFHPDFVEHALEKLSNLKECCYEPQKWLKQQYMSFYNSKQLSWKP 362
            G  LD +FFRLVDP+RF  D++E+ALEKL +LKECCYEPQKWLKQQY+SF +S QL WK 
Sbjct: 307  GSALDDKFFRLVDPTRFRSDYIEYALEKLFSLKECCYEPQKWLKQQYLSFCSSMQLPWKA 366

Query: 363  NISLEDGLVYVHRVQITPSKVYFRGPEANLSNRVVRRFIDDIDNFLRVSFVDEELDKIRS 422
            NISL+D LVYVHRVQITP+KVYFRGPEANLSNRVVRRFIDDIDNFLRVSFVDEELDKI S
Sbjct: 367  NISLDDSLVYVHRVQITPAKVYFRGPEANLSNRVVRRFIDDIDNFLRVSFVDEELDKIYS 426

Query: 423  IDLSPRTSTENSERTSVYDRILYVLRNGIVIGEKKFEFLAFSASQLRENSFWMFASREGL 482
             DLSPRTSTE  ERT VYDRIL VLRNGIVIG KKFEFLAFSASQLRENSFWMFASREGL
Sbjct: 427  SDLSPRTSTEKIERTRVYDRILSVLRNGIVIGGKKFEFLAFSASQLRENSFWMFASREGL 486

Query: 483  TAADIREWMGDFRQIRNVAKYAARLGQSFGSSRKTLCVEEHEIEVIPDVEVERKKTAYCF 542
             AADIREWMGDFR+IRNVAKYAARLGQSFGSSRKTLCVEE+EIEVIPDVEVERK   YCF
Sbjct: 487  NAADIREWMGDFREIRNVAKYAARLGQSFGSSRKTLCVEENEIEVIPDVEVERKNIKYCF 546

Query: 543  SDGIGKISETLAEKVAEKCGLFSHTPSAFQIRYAGYKGVVAIDPTSKKKLSLRKSMLKYM 602
            SDGIGKISE LAE VAEKCGL SHTPSAFQIRYAGYKGVVA+DPTSKKKLSLRKSMLKYM
Sbjct: 547  SDGIGKISEALAEMVAEKCGLISHTPSAFQIRYAGYKGVVAVDPTSKKKLSLRKSMLKYM 606

Query: 603  SLDTQLDVLSWSRYQPCFLNRQVINLLSTLGIRDRVFVKKQKEAIDQLDAILKDPSKALE 662
            SLDTQLDVLSWS+YQPCFLNRQVINLLSTLGI+D VFVK+QK+A+DQLD+IL+DPSKALE
Sbjct: 607  SLDTQLDVLSWSKYQPCFLNRQVINLLSTLGIQDDVFVKQQKKAVDQLDSILRDPSKALE 666

Query: 663  VLELISPGEMTSILKEMLLFYKPNEEPFLNMMLRTFRADKLLDLRTKSRIFVPKGRTMMG 722
            VLEL+SPGEMTS+LK+MLLFY P+EEPFLNMMLRTFRADKLLDLRTKSRIFVP+GRTMMG
Sbjct: 667  VLELMSPGEMTSVLKDMLLFYNPHEEPFLNMMLRTFRADKLLDLRTKSRIFVPEGRTMMG 726

Query: 723  CLDETRTLEYGQVFVHCSVPSRSSESNFVVTGKVVVAKNPCLHPGDVRLLDAVDVKALHH 782
            CLDETRTLEYGQVF+HCSVP RSSESNFVV GKVVVAKNPCLHPGDVR+LDAVDVK LHH
Sbjct: 727  CLDETRTLEYGQVFIHCSVPGRSSESNFVVKGKVVVAKNPCLHPGDVRVLDAVDVKTLHH 786

Query: 783  MVDCVVFPQKGERPHPNECSGSDLDGDLYFVCWDSELTCIRPAKPMSYKPAPTMQLDHDV 842
            MVDCVVFPQKG+RPHPNECSGSDLDGDLYFVCWD ELTCIRP KPMSY+PAPT+QLDHDV
Sbjct: 787  MVDCVVFPQKGKRPHPNECSGSDLDGDLYFVCWDPELTCIRPIKPMSYEPAPTIQLDHDV 846

Query: 843  TIEEVQEYLTMYMVNDGPGGIANAHTVYADKKPKKAMSAECIKLAKLFSIAVDFPKTGVP 902
            TIEEVQEY   YMVNDG GGIANAHTV+ADKKPKKAMSAECI+LAKLFSIAVDFPKTGVP
Sbjct: 847  TIEEVQEYFANYMVNDGLGGIANAHTVFADKKPKKAMSAECIELAKLFSIAVDFPKTGVP 906

Query: 903  ANLPRSLRVQEYPDFMEKPDKPTYVSNGVLGKLFRRVKDVSSDMNTIETFTKEVATECYD 962
            ANLPRSLRV EYPDFMEKP+K TY SNGV+GKLFR VKDVSSD+N IETFT+EVA +CYD
Sbjct: 907  ANLPRSLRVHEYPDFMEKPNKRTYTSNGVIGKLFRGVKDVSSDINNIETFTREVAAKCYD 966

Query: 963  PDMEVDGFENYLREAFDCKNRYDFKLGNLMDYYGIKTEPELVGGNILRVGKSFDKRNDLE 1022
            PDMEV GFENYL EAFD K+ YDFKLGNLMDYYGI+TEPELV GNILR+GKSFDKRND+E
Sbjct: 967  PDMEVMGFENYLSEAFDYKSMYDFKLGNLMDYYGIETEPELVSGNILRMGKSFDKRNDME 1026

Query: 1023 QISLAMKSLRKEARAWFNEKGSKSTH----DENEEFAKASAWYHVTYHPNYWGRYNEGMQ 1082
            QISLAM+SLRKEAR WFNEKGSKS +    DE++E+AKASAWYHVTYHPN+WGRYNEGMQ
Sbjct: 1027 QISLAMRSLRKEARGWFNEKGSKSIYNNNKDEDDEYAKASAWYHVTYHPNFWGRYNEGMQ 1086

Query: 1083 RDHFLSFPWCVSDKLIQIKREKKMCKISSSQVSSLIHKFGNLSLY 1118
            RDHFLSFPWCVS+KLIQIKRE    K+ SS VSSLIHKFG LS Y
Sbjct: 1087 RDHFLSFPWCVSEKLIQIKRE----KMESSPVSSLIHKFGKLSFY 1123

BLAST of Spg008341 vs. ExPASy TrEMBL
Match: A0A6J1CZM7 (RNA-dependent RNA polymerase OS=Momordica charantia OX=3673 GN=LOC111015673 PE=3 SV=1)

HSP 1 Score: 1809.3 bits (4685), Expect = 0.0e+00
Identity = 899/1118 (80.41%), Postives = 993/1118 (88.82%), Query Frame = 0

Query: 3    TIEIFGFPASVAADDVKEFLENHTGDGTVSTVRISKPKDENSRFAFATVRFTSKLAAEYV 62
            TIEI+GF   V A++VKEFLENHTGDGTVS VRI++ +DENSRF FATV+F+S+LAA +V
Sbjct: 4    TIEIYGFRRDVTAEEVKEFLENHTGDGTVSAVRIAEFRDENSRFTFATVQFSSELAAGHV 63

Query: 63   VAQAEAEERLWFESSYLKARGVEREMRAAAAEARRRGEVERIENVRIHLGNRISKERMSV 122
            V+QA AEERLW+ SSYLKAR  +R+ R           VER+EN+ +HLG++ISKE++SV
Sbjct: 64   VSQAAAEERLWYGSSYLKAREADRQNRRGGV------GVERMENLTLHLGSQISKEKLSV 123

Query: 123  IWRGENCCVEFGVGIRKLCFYLSYEFAEYKMELSFENILGVELRCPLDRPSNLLLIQLQG 182
            IW+GENC VEFG G+RKL  Y++    EYKME+ FE+IL V+LRCP D  S  LLIQLQ 
Sbjct: 124  IWKGENCSVEFGSGVRKLFVYVN---LEYKMEVWFESILRVQLRCPPDGASKFLLIQLQR 183

Query: 183  APRIFKKSSSPSSSSSS----------KESTGSRWIRAVDFTPSSCIGQSFALCLEVSPG 242
            APRIFKKSSS SSSSSS           +S+GSRWIR VDFTPSSCIGQSFALCLE+SPG
Sbjct: 184  APRIFKKSSSSSSSSSSSSSSRFYFKESQSSGSRWIRDVDFTPSSCIGQSFALCLELSPG 243

Query: 243  DQLLSFFETLVGYKETYGPFNLETGSSFSSTSNLVPIISPPQGFDISYKILFKINALVQH 302
            DQL SFF+TLVGY+E YGPFNLETGSSFSSTSNLVPI++PPQGFD S+KILFKINALVQH
Sbjct: 244  DQLPSFFKTLVGYEEIYGPFNLETGSSFSSTSNLVPIVTPPQGFDFSFKILFKINALVQH 303

Query: 303  GHLPGPLLDHEFFRLVDPSRFHPDFVEHALEKLSNLKECCYEPQKWLKQQYMSFYNSKQL 362
            G+LPGPLLD EFFRLVDPSRF P+++E ALEKL NLKEC YEPQKWLKQQY+S+YNSKQL
Sbjct: 304  GYLPGPLLDDEFFRLVDPSRFRPEYIERALEKLFNLKECFYEPQKWLKQQYLSYYNSKQL 363

Query: 363  SWKPNISLEDGLVYVHRVQITPSKVYFRGPEANLSNRVVRRFIDDIDNFLRVSFVDEELD 422
             WKP+ISL+DGLVYVHRVQITPSKVYFRGPEANLSNRVVR++ DDIDNF+RVSFVDEELD
Sbjct: 364  PWKPDISLDDGLVYVHRVQITPSKVYFRGPEANLSNRVVRQYSDDIDNFIRVSFVDEELD 423

Query: 423  KIRSIDLSPRTSTENSERTSVYDRILYVLRNGIVIGEKKFEFLAFSASQLRENSFWMFAS 482
            KIRSIDLSPRTST N ERT VYDRIL  LRNGIVIG+KKFEFLAFSASQLRENS WMFAS
Sbjct: 424  KIRSIDLSPRTSTANGERTRVYDRILSTLRNGIVIGDKKFEFLAFSASQLRENSVWMFAS 483

Query: 483  REGLTAADIREWMGDFRQIRNVAKYAARLGQSFGSSRKTLCVEEHEIEVIPDVEVERKKT 542
            REGL+AADIREWMGDFRQIRNVAKYAARLGQSFGSSRKTLCVEEHEIEVI DVEVE    
Sbjct: 484  REGLSAADIREWMGDFRQIRNVAKYAARLGQSFGSSRKTLCVEEHEIEVITDVEVETNNV 543

Query: 543  AYCFSDGIGKISETLAEKVAEKCGLFSHTPSAFQIRYAGYKGVVAIDPTSKKKLSLRKSM 602
             YCFSDGIGKISETLAEKVAE+CGL +HTPSAFQIRYAGYKGVVAIDPTS+KKLSLRKSM
Sbjct: 544  TYCFSDGIGKISETLAEKVAERCGLINHTPSAFQIRYAGYKGVVAIDPTSEKKLSLRKSM 603

Query: 603  LKYMSLDTQLDVLSWSRYQPCFLNRQVINLLSTLGIRDRVFVKKQKEAIDQLDAILKDPS 662
            LKY SLDTQLDVL+WS+Y PCFLNRQVINLLSTLGI+DRVF KKQKEAI QL+ ILKDPS
Sbjct: 604  LKYKSLDTQLDVLAWSKYLPCFLNRQVINLLSTLGIKDRVFEKKQKEAIYQLNCILKDPS 663

Query: 663  KALEVLELISPGEMTSILKEMLLFYKPNEEPFLNMMLRTFRADKLLDLRTKSRIFVPKGR 722
            +ALEVLEL+SPGEMTS+LKEMLL+YKPN+EPFLNMMLRTFRADKLLDLRTKSRIFVPKGR
Sbjct: 664  RALEVLELMSPGEMTSVLKEMLLYYKPNKEPFLNMMLRTFRADKLLDLRTKSRIFVPKGR 723

Query: 723  TMMGCLDETRTLEYGQVFVHCSVPSRSSESNFVVTGKVVVAKNPCLHPGDVRLLDAVDVK 782
            TMMGCLDET TL+YGQVFV CSVP RSSESNF+  GKVVVAKNPCLHPGD+R+L+AVDVK
Sbjct: 724  TMMGCLDETGTLKYGQVFVQCSVPGRSSESNFIPEGKVVVAKNPCLHPGDMRVLNAVDVK 783

Query: 783  ALHHMVDCVVFPQKGERPHPNECSGSDLDGDLYFVCWDSELTCIRPAKPMSYKPAPTMQL 842
            ALHHMVDCVVFPQKG+RPHPNECSGSDLDGDLYFVCWD EL CIRP KPMSYKPAPT+QL
Sbjct: 784  ALHHMVDCVVFPQKGQRPHPNECSGSDLDGDLYFVCWDPELNCIRPVKPMSYKPAPTIQL 843

Query: 843  DHDVTIEEVQEYLTMYMVNDGPGGIANAHTVYADKKPKKAMSAECIKLAKLFSIAVDFPK 902
            DHDVTIEEVQEY+  YMVNDG G IANAHTV+AD++P+KAMSA+CIKLAKLFSIAVDFPK
Sbjct: 844  DHDVTIEEVQEYMANYMVNDGLGAIANAHTVFADREPRKAMSAKCIKLAKLFSIAVDFPK 903

Query: 903  TGVPANLPRSLRVQEYPDFMEKPDKPTYVSNGVLGKLFRRVKDVSSDMNTIETFTKEVAT 962
            TGVPANLP  LRV E+PDFMEK D+P Y S GV+GKLFR VKDVS + NTIET+T+EVA 
Sbjct: 904  TGVPANLPPFLRVHEFPDFMEKLDRPAYESKGVIGKLFRSVKDVSFNTNTIETYTREVAA 963

Query: 963  ECYDPDMEVDGFENYLREAFDCKNRYDFKLGNLMDYYGIKTEPELVGGNILRVGKSFDKR 1022
            +CYDPDME+DGFE YL EAFD K RYD+KLGNLMDYYGI+TEPELV GNILRVGKSFDKR
Sbjct: 964  KCYDPDMEIDGFEKYLNEAFDFKTRYDYKLGNLMDYYGIRTEPELVSGNILRVGKSFDKR 1023

Query: 1023 NDLEQISLAMKSLRKEARAWFNEKGSK------STHDENEEFAKASAWYHVTYHPNYWGR 1082
             D+EQIS AM+SLRKEARAWFNEKGSK         DE+EEFAKASAWYHVTYH +YWGR
Sbjct: 1024 TDMEQISHAMRSLRKEARAWFNEKGSKPRCDDDDNGDEDEEFAKASAWYHVTYHQSYWGR 1083

Query: 1083 YNEGMQRDHFLSFPWCVSDKLIQIKREKKMCKISSSQV 1105
            YNEGM+RDHFLSFPWCVSDKLIQIK+ KK+C+ +SS +
Sbjct: 1084 YNEGMERDHFLSFPWCVSDKLIQIKK-KKICQENSSPI 1111

BLAST of Spg008341 vs. TAIR 10
Match: AT1G14790.1 (RNA-dependent RNA polymerase 1 )

HSP 1 Score: 1245.0 bits (3220), Expect = 0.0e+00
Identity = 634/1098 (57.74%), Postives = 804/1098 (73.22%), Query Frame = 0

Query: 3    TIEIFGFPASVAADDVKEFLENHTGDGTVSTVRISKPKDENSRFAFATVRFTSKLAAEYV 62
            TI++FGFP  V+A++VK+FLE  TG GTV  +++ +PK    R  +A V+FTS+     +
Sbjct: 4    TIQVFGFPNGVSAEEVKKFLERLTGSGTVYAIKVRQPKKGGPR-VYAIVQFTSERHTRLI 63

Query: 63   VAQAEAEERLWFESSYLKARGVEREMRAAAAEARRRGEVERIENVRIHLGNRISKERMSV 122
            +    A ERL++  SYLKA  VE+++       + R  +  I  +++  G ++S ++   
Sbjct: 64   I--TAAAERLYYGRSYLKAFEVEQDI-----VPKPRASLHTISGLKMFFGCQVSTKKFLT 123

Query: 123  IWRGENCCVEFGVGIRKLCFYLSYEFAEYKMELSFENILGVELRCPLDRPSNLLLIQLQG 182
            +W  ++ CV FG+G+RKL F  S+   +Y++ELS+ENI  ++L  P  R S  L+IQ+ G
Sbjct: 124  LWSAQDVCVSFGIGMRKLHFSFSWYQKDYRLELSYENIWQIDLHSPQGRSSKFLVIQVIG 183

Query: 183  APRIFKKSSSPSS-----SSSSKESTGSRWIRAVDFTPSSCIGQSFALCLEVSPGDQLLS 242
            AP+IF+K   P +          + +  +WIR  DFT SSCIGQS A CLE+     +  
Sbjct: 184  APKIFEKEDQPINLLFGIMDFYSDGSDEQWIRTTDFTSSSCIGQSTAFCLELPVHLNVPD 243

Query: 243  FFETLVGYKE-TYGPFNLETGSSFSSTSN-LVPIISPPQGFDISYKILFKINALVQHGHL 302
            F E    Y E     F +E+GSS+SS +N LVP++ PP GF + ++ILFK+N LVQ+  L
Sbjct: 244  FRENFANYAEHRASSFLIESGSSYSSNANTLVPVVDPPPGFSLPFEILFKLNTLVQNACL 303

Query: 303  PGPLLDHEFFRLVDPSRFHPDFVEHALEKLSNLKECCYEPQKWLKQQYMSFYNSKQLSWK 362
             GP LD +F+RL++  ++    ++H LEKL +L ECCYEP  WL+ +Y  + +  +L   
Sbjct: 304  SGPALDLDFYRLLNQKKYDRALIDHCLEKLFHLGECCYEPAHWLRDEYKKWISKGKLPLS 363

Query: 363  PNISLEDGLVYVHRVQITPSKVYFRGPEANLSNRVVRRFIDDIDNFLRVSFVDEELDKIR 422
            P ISL+DGLVY++RVQ+TP++VYF GPE N+SNRV+R +   I+NFLRVSFVDE+L+K+R
Sbjct: 364  PTISLDDGLVYMYRVQVTPARVYFSGPEVNVSNRVLRHYSKYINNFLRVSFVDEDLEKVR 423

Query: 423  SIDLSPRTSTENSERTSVYDRILYVLRNGIVIGEKKFEFLAFSASQLRENSFWMFASREG 482
            S+DLSPR+ST+   RT +YDRI  VLR+GIVIG+KKFEFLAFS+SQLRENS WMFA  + 
Sbjct: 424  SMDLSPRSSTQ--RRTKLYDRIYSVLRDGIVIGDKKFEFLAFSSSQLRENSAWMFAPIDR 483

Query: 483  LTAADIREWMGDFRQIRNVAKYAARLGQSFGSSRKTLCVEEHEIEVIPDVEVERKKTAYC 542
            +TAA IR WMGDF  IRNVAKYAARLGQSF SSR+TL V   EIEVIPDVE+    T Y 
Sbjct: 484  ITAAHIRAWMGDFDHIRNVAKYAARLGQSFSSSRETLNVRSDEIEVIPDVEIISLGTRYV 543

Query: 543  FSDGIGKISETLAEKVAEKCGLFSHTPSAFQIRYAGYKGVVAIDPTSKKKLSLRKSMLKY 602
            FSDGIGKIS   A KVA KCGL   +PSAFQIRY GYKGVVA+DP S KKLSLRKSM K+
Sbjct: 544  FSDGIGKISAEFARKVARKCGLTEFSPSAFQIRYGGYKGVVAVDPNSSKKLSLRKSMSKF 603

Query: 603  MSLDTQLDVLSWSRYQPCFLNRQVINLLSTLGIRDRVFVKKQKEAIDQLDAILKDPSKAL 662
             S +T+LDVL+WS+YQPC++NRQ+I LLSTLG+ D VF KKQ+E +D+LDAIL  P +A 
Sbjct: 604  ESENTKLDVLAWSKYQPCYMNRQLITLLSTLGVTDSVFEKKQREVVDRLDAILTHPLEAH 663

Query: 663  EVLELISPGEMTSILKEMLLF-YKPNEEPFLNMMLRTFRADKLLDLRTKSRIFVPKGRTM 722
            E L L++PGE T+ILK ++L  YKP+ EPFL+MML+ FRA KLL+LRTK+RIF+  GR+M
Sbjct: 664  EALGLMAPGENTNILKALILCGYKPDAEPFLSMMLQNFRASKLLELRTKTRIFISGGRSM 723

Query: 723  MGCLDETRTLEYGQVFVHCSVPSRSSESNFVVTGKVVVAKNPCLHPGDVRLLDAVDVKAL 782
            MGCLDETRTLEYGQV V  S P R     F++TG VVVAKNPCLHPGDVR+L AV+V AL
Sbjct: 724  MGCLDETRTLEYGQVVVQYSDPMRPGR-RFIITGPVVVAKNPCLHPGDVRVLQAVNVPAL 783

Query: 783  HHMVDCVVFPQKGERPHPNECSGSDLDGDLYFVCWDSELTCIRPAKPMSYKPAPTMQLDH 842
            +HMVDCVVFPQKG RPHPNECSGSDLDGD+YFVCWD EL   R ++PM Y P PT  LDH
Sbjct: 784  NHMVDCVVFPQKGLRPHPNECSGSDLDGDIYFVCWDQELVPPRTSEPMDYTPEPTQILDH 843

Query: 843  DVTIEEVQEYLTMYMVNDGPGGIANAHTVYADKKPKKAMSAECIKLAKLFSIAVDFPKTG 902
            DVTIEEV+EY   Y+VND  G IANAHT +ADK+P KA S  CI+LAK FS AVDFPKTG
Sbjct: 844  DVTIEEVEEYFANYIVNDSLGIIANAHTAFADKEPLKAFSDPCIELAKKFSTAVDFPKTG 903

Query: 903  VPANLPRSLRVQEYPDFMEKPDKPTYVSNGVLGKLFRRVKDVSSDMNTIETFTKEVATEC 962
            V A +P+ L V+EYPDFMEKPDKPTY S  V+GKLFR VK+ +  + +I++FT +VA++ 
Sbjct: 904  VAAVIPQHLYVKEYPDFMEKPDKPTYESKNVIGKLFREVKERAPPLISIKSFTLDVASKS 963

Query: 963  YDPDMEVDGFENYLREAFDCKNRYDFKLGNLMDYYGIKTEPELVGGNILRVGKSFDKRND 1022
            YD DMEVDGFE Y+ EAF  K  YDFKLGNLMDYYGIKTE E++ G I+R+ KSF KR D
Sbjct: 964  YDKDMEVDGFEEYVDEAFYQKANYDFKLGNLMDYYGIKTEAEILSGGIMRMSKSFTKRRD 1023

Query: 1023 LEQISLAMKSLRKEARAWFNEKGSKSTHDENEEFAKASAWYHVTYHPNYWGRYNEGMQRD 1082
             E I  A+++LRKE  + FN     ++ +E  E AKASAWYHVTYH +YWG YNEG+ RD
Sbjct: 1024 AESIGRAVRALRKETLSLFN-----ASEEEENESAKASAWYHVTYHSSYWGLYNEGLNRD 1083

Query: 1083 HFLSFPWCVSDKLIQIKR 1093
            HFLSF WCV DKL++IK+
Sbjct: 1084 HFLSFAWCVYDKLVRIKK 1085

BLAST of Spg008341 vs. TAIR 10
Match: AT4G11130.1 (RNA-dependent RNA polymerase 2 )

HSP 1 Score: 752.3 bits (1941), Expect = 5.6e-217
Identity = 449/1149 (39.08%), Postives = 661/1149 (57.53%), Query Frame = 0

Query: 2    ATIEIFGFPASVAADDVKEFLENHTGDGTVSTVRISKPKDENSRFAFATVRFTSKLAAEY 61
            +T++I   P ++ AD++  FLE H G+ TV  + I   +D      FA V+FT+ L  + 
Sbjct: 10   STVKISNVPQTIVADELLRFLELHLGEDTVFALEIPTTRDNWKPRDFARVQFTT-LEVKS 69

Query: 62   VVAQAEAEERLWFESSYLKARGVEREMRAAAAEARRRGEVERIENVRIHLGNRISKERMS 121
                  ++ +L F++  L+      ++     + R+     R++++ + +G   S E+  
Sbjct: 70   RAQLLSSQSKLLFKTHNLRLSEAYDDIIPRPVDPRK-----RLDDIVLTVGFPESDEKRF 129

Query: 122  VI---WRGENCCVEFGVGIRKLCFYLSYEFAEYKMELSFENILGVELRCPLDRPSNL--L 181
                 W G  C +      R++ F++      YK+E+ FE+I+     C     S +   
Sbjct: 130  CALEKWDGVRCWIL--TEKRRVEFWVWESGDCYKIEVRFEDIIETLSCCVNGDASEIDAF 189

Query: 182  LIQLQGAPRIFKKSSSPSSSSSS-------KESTGSRWIRAVDFTPSSCIGQSFALCLEV 241
            L++L+  P++FK+ +   ++          KE     WIR  DF+ S  IG S   CLEV
Sbjct: 190  LLKLKYGPKVFKRVTVHIATKFKSDRYRFCKEDFDFMWIRTTDFSGSKSIGTSTCFCLEV 249

Query: 242  SPGDQLLSFFETLVGYKETYGPFNLETGSSFSSTSNLVPIISPP-QGFDISYKILFKINA 301
              G  +L  F  L  Y+E         G +F+S + +VP+++    G +  Y+ILF++NA
Sbjct: 250  HNGSTMLDIFSGLPYYREDTLSLTYVDGKTFASAAQIVPLLNAAILGLEFPYEILFQLNA 309

Query: 302  LVQHGHLP-GPLLDHEFFRLVDPSRFHPDFVEHALEKLSNLKECCYEPQKWLKQQYMSFY 361
            LV    +      D E  +++         V   L+KL      CY+P  ++K Q  S  
Sbjct: 310  LVHAQKISLFAASDMELIKILRGMSLETALV--ILKKLHQQSSICYDPVFFVKTQMQSVV 369

Query: 362  NSKQLSWKPNIS----LEDGLVYVHRVQITPSKVYFRGPEANLSNRVVRRFIDDIDNFLR 421
              K++   P  +     E  ++   R  +TPSK+Y  GPE   +N VV+ F + + +F+R
Sbjct: 370  --KKMKHSPASAYKRLTEQNIMSCQRAYVTPSKIYLLGPELETANYVVKNFAEHVSDFMR 429

Query: 422  VSFVDEELDKIRSIDLSPRTST---ENSERTSVYDRILYVLRNGIVIGEKKFEFLAFSAS 481
            V+FV+E+  K+ +  LS  +         RT++Y+R+L +L  GI +G K+FEFLAFSAS
Sbjct: 430  VTFVEEDWSKLPANALSVNSKEGYFVKPSRTNIYNRVLSILGEGITVGPKRFEFLAFSAS 489

Query: 482  QLRENSFWMFASREGLTAADIREWMGDFRQIRNVAKYAARLGQSFGSSRKTLCVEEHEIE 541
            QLR NS WMFAS E + A DIREWMG FR+IR+++K AAR+GQ F +SR+TL V   ++E
Sbjct: 490  QLRGNSVWMFASNEKVKAEDIREWMGCFRKIRSISKCAARMGQLFSASRQTLIVRAQDVE 549

Query: 542  VIPDVEVERKKTAYCFSDGIGKISETLAEKVAEKCGLFSHTPSAFQIRYAGYKGVVAIDP 601
             IPD+EV      YCFSDGIGKIS   A++VA+KCGL SH PSAFQIRY GYKGV+A+D 
Sbjct: 550  QIPDIEVTTDGADYCFSDGIGKISLAFAKQVAQKCGL-SHVPSAFQIRYGGYKGVIAVDR 609

Query: 602  TSKKKLSLRKSMLKYMSLDTQLDVLSWSRYQPCFLNRQVINLLSTLGIRDRVFVKKQKEA 661
            +S +KLSLR SMLK+ S +  L+V  W+   PCFLNR++I LLSTLGI D +F   Q   
Sbjct: 610  SSFRKLSLRDSMLKFDSNNRMLNVTRWTESMPCFLNREIICLLSTLGIEDAMFEAMQAVH 669

Query: 662  IDQLDAILKDPSKALEVLELISPGEMTSILKEMLL-FYKPNEEPFLNMMLRTFRADKLLD 721
            +  L  +L+D   AL VL+ +S     ++L +MLL  Y P+ EP+L+MMLR     +L +
Sbjct: 670  LSMLGNMLEDRDAALNVLQKLSGENSKNLLVKMLLQGYAPSSEPYLSMMLRVHHESQLSE 729

Query: 722  LRTKSRIFVPKGRTMMGCLDETRTLEYGQVFVHCSVPS------------RSSESNFVVT 781
            L+++ RI VPKGR ++GC+DE   LEYGQV+V  ++              +  E   VV 
Sbjct: 730  LKSRCRILVPKGRILIGCMDEMGILEYGQVYVRVTLTKAELKSRDQSYFRKIDEETSVVI 789

Query: 782  GKVVVAKNPCLHPGDVRLLDAVDVKALHH--MVDCVVFPQKGERPHPNECSGSDLDGDLY 841
            GKVVV KNPCLHPGD+R+LDA+          +DC++FPQKGERPHPNECSG DLDGD +
Sbjct: 790  GKVVVTKNPCLHPGDIRVLDAIYEVHFEEKGYLDCIIFPQKGERPHPNECSGGDLDGDQF 849

Query: 842  FVCWDSELTCIRPAKPMSYKPAPTMQLDHDVTIEEVQEYLTMYMVNDGPGGIANAHTVYA 901
            FV WD ++       PM Y  +    +DHDVT+EE+ ++   YM++D  G I+ AH V+A
Sbjct: 850  FVSWDEKIIPSEMDPPMDYAGSRPRLMDHDVTLEEIHKFFVDYMISDTLGVISTAHLVHA 909

Query: 902  DKKPKKAMSAECIKLAKLFSIAVDFPKTGVPANLPRSLRVQEYPDFMEKPDKPTYVSNGV 961
            D+ P+KA S +C++LA L S AVDF KTG PA +P +L+ +E+PDF+E+ +KPTY+S  V
Sbjct: 910  DRDPEKARSQKCLELANLHSRAVDFAKTGAPAEMPYALKPREFPDFLERFEKPTYISESV 969

Query: 962  LGKLFRRVKDVSSDMNTIETFTKEVATECYDPDMEVDGFENYLREAFDCKNRYDFKLGNL 1021
             GKL+R VK   S +   +   +   T  YD  +E  GFE+++  A   ++ Y  KL +L
Sbjct: 970  FGKLYRAVK---SSLAQRKPEAESEDTVAYDVTLEEAGFESFIETAKAHRDMYGEKLTSL 1029

Query: 1022 MDYYGIKTEPELVGGNILRVGKSFDKRND------LEQISLAMKSLRKEARAWFNEKGSK 1081
            M YYG   E E++ G IL+  + +  R++       ++I+L++K L KEA  WF     K
Sbjct: 1030 MIYYGAANEEEILTG-ILKTKEMYLARDNRRYGDMKDRITLSVKDLHKEAMGWF----EK 1089

Query: 1082 STHDENEEFAKASAWYHVTYHPNYWGRYNEGMQRDHFLSFPWCVSDKLIQIKREKKMCKI 1109
            S  DE ++   ASAWY+VTY+PN+        ++  FLSFPW V D L+ IK E    + 
Sbjct: 1090 SCEDEQQKKKLASAWYYVTYNPNHRD------EKLTFLSFPWIVGDVLLDIKAENAQRQS 1131

BLAST of Spg008341 vs. TAIR 10
Match: AT3G49500.1 (RNA-dependent RNA polymerase 6 )

HSP 1 Score: 577.0 bits (1486), Expect = 3.2e-164
Identity = 406/1089 (37.28%), Postives = 579/1089 (53.17%), Query Frame = 0

Query: 96   RRRGEVE-RIENVRIHLGNRISKERMSVIWRGENCCVEFGV----GIRKLCFYLSYEFA- 155
            RRR  V  ++  + + +G  +S++   V WR E   V+F V       K CF  S  F+ 
Sbjct: 123  RRRTTVPYKLAGITLEIGTLVSRDDFFVSWRAEG--VDFLVDPFDNTCKFCFRKSTAFSF 182

Query: 156  -----------EYKMELSFENILGVELRCPLDRPSNLLLIQLQGAPRIFKKSSSP---SS 215
                       +YK+EL   +I  V     L     +L++QL  +PR++ +++      +
Sbjct: 183  KDAVMHAVINCDYKLELLVRDIQTVRQYKTLH--GFVLILQLASSPRVWYRTADDDIYDT 242

Query: 216  SSSSKESTGSRWIRAVDFTPSSCIGQSFALCLEVSPGDQ-----LLSFFE-TLVGYKETY 275
                       WIR  DFT    IG+  +  + +SP  +      L +F    V  +   
Sbjct: 243  VPGDLLDDDDPWIRTTDFTQVGAIGRCHSYRVLISPRYENKLRTALDYFRMRRVQEERVR 302

Query: 276  GPFNLETGSSFSS-TSNLVPIISPPQGFDISYKILFKINALVQHGHLPGPLLDHEFFRLV 335
             P  +     F    S+    I   +G  IS++I+F +N+++  G      L   FF L+
Sbjct: 303  WPPRIRNEPCFGEPVSDHFFCIHHKEG--ISFEIMFLVNSVLHRGVFNQFQLTERFFDLL 362

Query: 336  DPSRFHPDFVEHA-LEKLSNLKECCYEPQKWLK--QQYMSFYNSKQLSWKPNISLEDGLV 395
               R  P  V  A L+ L   K   ++  K LK  Q+++   N K L    +    + + 
Sbjct: 363  ---RNQPKDVNIASLKHLCTYKRPVFDAYKRLKLVQEWIQ-KNPKLLG---SHEQSEDIS 422

Query: 396  YVHRVQITPSKVYFRGPEANLSNRVVRRFIDDIDNFLRVSFVDEELDKIRSIDLS----- 455
             + R+ ITP++ Y   PE  LSNRV+RR+    + FLRV+F+DE +  I S  LS     
Sbjct: 423  EIRRLVITPTRAYCLPPEVELSNRVLRRYKAVAERFLRVTFMDESMQTINSNVLSYFVAP 482

Query: 456  ---PRTSTENSERTSVYDRILYVLRNGIVIGEKKFEFLAFSASQLRENSFWMFASREGLT 515
                 TS+  S++T V+ R+  +L +G  +  +K+ FLAFSA+QLR+ S W FA      
Sbjct: 483  IVKDLTSSSFSQKTYVFKRVKSILTDGFKLCGRKYSFLAFSANQLRDRSAWFFAEDGKTR 542

Query: 516  AADIREWMGDFRQIRNVAKYAARLGQSFGSSRKTLCVEEHEIEV-IPDVEVERKKTAYCF 575
             +DI+ WMG F+  +NVAK AAR+G  F S+  T+ V  HE++  +PD+E    +  Y F
Sbjct: 543  VSDIKTWMGKFKD-KNVAKCAARMGLCFSSTYATVDVMPHEVDTEVPDIE----RNGYVF 602

Query: 576  SDGIGKISETLAEKVAEKCGLFSH-TPSAFQIRYAGYKGVVAIDPTSKK--KLSLRKSML 635
            SDGIG I+  LA++V EK  L  H +P A+QIRYAG+KGVVA  P+     +L+LR SM 
Sbjct: 603  SDGIGTITPDLADEVMEKLKLDVHYSPCAYQIRYAGFKGVVARWPSKSDGIRLALRDSMK 662

Query: 636  KYMSLDTQLDVLSWSRYQPCFLNRQVINLLSTLGIRDRVFVKKQKEAIDQLDAILKDPSK 695
            K+ S  T L++ SW+R+QP FLNRQ+I LLS LG+ D +F   Q+  + +L+ IL D   
Sbjct: 663  KFFSKHTILEICSWTRFQPGFLNRQIITLLSVLGVPDEIFWDMQESMLYKLNRILDDTDV 722

Query: 696  ALEVLELISPGEMTSILKEMLLFYKPNEEPFLNMMLRTFRADKLLDLRTKSRIFVPKGRT 755
            A EVL      +  +    +   +KP  EP L  ML + R  +L  LR KSRIFV  GR 
Sbjct: 723  AFEVLTASCAEQGNTAAIMLSAGFKPKTEPHLRGMLSSVRIAQLWGLREKSRIFVTSGRW 782

Query: 756  MMGCLDETRTLEYGQVFVHCSVP----------SRSSESNF---VVTGKVVVAKNPCLHP 815
            +MGCLDE   LE+GQ F+  S P          SR  E+     VV G V +AKNPCLHP
Sbjct: 783  LMGCLDEAGILEHGQCFIQVSKPSIENCFSKHGSRFKETKTDLEVVKGYVAIAKNPCLHP 842

Query: 816  GDVRLLDAVDVKALHHMVDCVVFPQKGERPHPNECSGSDLDGDLYFVCWDSELTCIRPAK 875
            GDVR+L+AVDV  LHHM DC++FPQKG+RPH NE SGSDLDGDLYFV WD +L  I P +
Sbjct: 843  GDVRILEAVDVPQLHHMYDCLIFPQKGDRPHTNEASGSDLDGDLYFVAWDQKL--IPPNR 902

Query: 876  ----PMSYKPAPTMQLDHDVTIEEVQEYLTMYMVNDGPGGIANAHTVYADKKPKKAMSAE 935
                 M Y  A    L   V  +++ ++    + N+  G I NAH V+AD+    AM  E
Sbjct: 903  KSYPAMHYDAAEEKSLGRAVNHQDIIDFFARNLANEQLGTICNAHVVHADRSEYGAMDEE 962

Query: 936  CIKLAKLFSIAVDFPKTGVPANLPRSLRVQEYPDFMEKPDKPTYVSNGVLGKLFRRVKDV 995
            C+ LA+L + AVDFPKTG   ++P  L+ + YPDFM K D  TY SN +LG+L+RRVK+V
Sbjct: 963  CLLLAELAATAVDFPKTGKIVSMPFHLKPKLYPDFMGKEDYQTYKSNKILGRLYRRVKEV 1022

Query: 996  -SSDMNTIETFTKEVATECYDPDMEVDGFENYLREAFDCKNRYDFKLGNLMDYYGIKTEP 1055
               D       + + +   YD  +E+ GFE+ + EA+  K  YD +L  L+  Y ++ E 
Sbjct: 1023 YDEDAEASSEESTDPSAIPYDAVLEIPGFEDLIPEAWGHKCLYDGQLIGLLGQYKVQKEE 1082

Query: 1056 ELVGGNILRVGKSFDKRND--LEQISLAMKSLRKEARAWFNEKGSKSTHDENEE-----F 1112
            E+V G+I  + K   K+     E++  +  SL+KE R  F E      +   EE      
Sbjct: 1083 EIVTGHIWSMPKYTSKKQGELKERLKHSYNSLKKEFRKVFEETIPDHENLSEEEKNILYE 1142

BLAST of Spg008341 vs. TAIR 10
Match: AT2G19930.1 (RNA-dependent RNA polymerase family protein )

HSP 1 Score: 132.1 bits (331), Expect = 2.8e-30
Identity = 182/741 (24.56%), Postives = 305/741 (41.16%), Query Frame = 0

Query: 333 YEPQKW--LKQQYMSFYNSKQLSWKPNISLEDGLVYVHRVQITPSKVY-------FRGPE 392
           YE   W  L + Y    + + L W      + G  + ++  + P+  Y        +GP 
Sbjct: 174 YEAAVWDRLGRHYCPQKDRRMLQW------DSGKTHYYQCNVAPNGSYTFKVLSALQGPL 233

Query: 393 ANLSNRVVRRFIDDIDNFLRVSFVDEELDKIRSIDLSPRTSTENSERTSVYDRILYVLRN 452
              +   + + + D DN L V F           D+   +ST + +    Y  I    +N
Sbjct: 234 LEHTGTHLHKVLGD-DNVLTVKFA----------DVQKSSSTYSIDHYFTYKGI---AKN 293

Query: 453 GIVIGEKKFEFLAFSASQLRE-------------------NSFWMFASREGLTAADIREW 512
           GI+IG ++++F  F      E                    +F+   +   LT   I E 
Sbjct: 294 GIMIGLRRYQFFVFKDGGKEEKKKDLSTKKVKCYFIRTDSTAFYDMQNPYILTGKSIYEA 353

Query: 513 MGDFRQIR---NVAKYAARLGQSFGSSRKTLCVEEHEI--EVIPDVE---------VERK 572
              F  +     +A Y AR      S  KTL V+   I  + I D+          +++ 
Sbjct: 354 RMHFMHVHRAPTLANYMARFSLIL-SKTKTLEVDMTGITFDQIDDIHCHDQDGKDVLDKN 413

Query: 573 KTAYCFSDGIGKISETLA---------------EKVAEKCGLFSHTPSAFQIR--YAGY- 632
           K     SDG G ISE LA               E + E C  +   P   Q R  Y GY 
Sbjct: 414 KKPCIHSDGTGYISEDLARMCPLNIFKGKCLRSESIQEAC--YQDPPLLIQFRMFYDGYA 473

Query: 633 -KGVVAID-PTSKKKLSLRKSMLKY--------MSLDTQLDVLSWSR-YQPCFLNRQVIN 692
            KG   ++     + + +R SM+K          S    L+V++ S   +   L++ ++ 
Sbjct: 474 VKGTFLLNKKLCPRTVQVRPSMIKVSKDPSLSNFSTFNALEVVTTSNPPKRTKLSKNLVA 533

Query: 693 LLSTLGIRDRVFVKKQKEAIDQLDAILKDPSKALEVLELISPGEM-TSILKEMLLFYKPN 752
           LLS  GI +  F+      +++  +I  +   AL     ++ GEM      +M+L   P 
Sbjct: 534 LLSYGGIPNEFFLDILLNTLEESKSIFYNKRAALNA--ALNYGEMDDQNAAQMILVGIPL 593

Query: 753 EEPFLNMMLRTFRADKLLDLRTKSRIFVPKGRTMMGCLDETRTLEYGQVFVHCSVPSRSS 812
           +EP L   L      +  DL+   ++ V +   +MG +D T  L+  +V   C +     
Sbjct: 594 DEPHLKNYLSILLKTEKNDLKA-GKLPVTESYYLMGTVDPTGALKEDEV---CVILESGQ 653

Query: 813 ESNFVVTGKVVVAKNPCLHPGDVRLLDAVDVKALHHMVD----CVVFPQKGERPHPNECS 872
                ++G+V+V +NP LH GD+ +L A  VKAL   V      V FPQKG R   +E +
Sbjct: 654 -----ISGEVLVYRNPGLHFGDIHILKATYVKALEEYVGNSKFAVFFPQKGPRSLGDEIA 713

Query: 873 GSDLDGDLYFVCWDSE-LTCIRPAKP-MSYKP--------APTMQLDHDVTIEEVQEYLT 932
           G D DGD+YF+  + E L   +P++P +S  P        AP+     ++  E  + +LT
Sbjct: 714 GGDFDGDMYFISRNPELLENFKPSEPWVSLTPPSKSNSGRAPSQLSPEELEEELFEMFLT 773

Query: 933 M-YMVNDGPGGIANAHTVYADK---------KPKKAMSAECIKLAKLFSIAVDFPKTGVP 971
             +  ++  G  A++     D+         + K  M  + ++L  ++  A+D PK G  
Sbjct: 774 AGFHASNVIGIAADSWLTIMDRFLILGDDRAEEKAEMKKKMLELIDIYYDALDAPKKGDK 833

BLAST of Spg008341 vs. TAIR 10
Match: AT2G19910.1 (RNA-dependent RNA polymerase family protein )

HSP 1 Score: 112.1 bits (279), Expect = 2.9e-24
Identity = 126/507 (24.85%), Postives = 218/507 (43.00%), Query Frame = 0

Query: 565  FQIRYAGYKGVVAIDPTSKK----KLSLRKSMLKY--------MSLDTQLDVLSWSR-YQ 624
            F+I Y GY  V     T+KK     + +R SM+K         +S    L+V++ S   +
Sbjct: 455  FRIFYNGY-AVKGTFLTNKKLPPRTVQVRPSMIKVYEDRTLSNLSTFNSLEVVTTSNPPR 514

Query: 625  PCFLNRQVINLLSTLGIRDRVFVKKQKEAIDQLDAILKDPSKALEVLELISPGEMTSILK 684
               L+R ++ LLS  G+ +  F+   +  +++   I      A +        + T+   
Sbjct: 515  KARLSRNLVALLSYGGVPNDFFLNILRNTLEESKTIFYSERAAFKAAINYGDDQYTA--- 574

Query: 685  EMLLFYKPNEEPFLNMMLRTFRADKLLDLRTKSRIFVPKGRTMMGCLDETRTLEYGQVFV 744
            +M+L   P +EP+L   L      +   L+   R  + +   +MG +D T  L+  ++  
Sbjct: 575  DMILVGIPLDEPYLKDRLSYLLKTERNALKA-GRFPIDESYYIMGTVDPTGELKENEI-- 634

Query: 745  HCSVPSRSSESNFVVTGKVVVAKNPCLHPGDVRLLDAVDVKALHHMVD----CVVFPQKG 804
             C +          ++G V+V +NP LH GD+ +L A  VKAL   V      V FPQKG
Sbjct: 635  -CVILHSGQ-----ISGDVLVYRNPGLHFGDIHVLKATYVKALEDYVGNAKFAVFFPQKG 694

Query: 805  ERPHPNECSGSDLDGDLYFVCWDSE-LTCIRPAKP--MSYKPAPTM--QLDHDVTIEEVQ 864
             R   +E +G D DGD+YF+  + + L   +P++P   S KP+     +   +++ EE++
Sbjct: 695  PRSLGDEIAGGDFDGDMYFISRNPKLLEHFKPSEPWVSSSKPSKIYCGRKPSELSEEELE 754

Query: 865  EYL-------------TMYMVNDGPGGIANAHTVYADK--KPKKAMSAECIKLAKLFSIA 924
            E L              + M  D   GI +      D+  K K       +KL  ++  A
Sbjct: 755  EELFKMFLKARFCKRDVIGMAADCWLGIMDPFLTLGDESAKEKYERKKNILKLIDIYYDA 814

Query: 925  VDFPKTGVPANLPRSLRVQEYPDFMEKPDKPTYVSNGVLGKLFRRVKDVSSDMNTIETFT 984
            +D PK G   +LP  L ++ +P +ME+  K  + S  +LG +F    D     N  E   
Sbjct: 815  LDAPKKGAKVDLPPDLEIKNFPHYMERDPKRDFRSTSILGLIF----DTVDSHNAEEPPP 874

Query: 985  KEVATECYDPDMEVDGFENYLREAFDCKNRYDFKLGNLMDYYGIKTEPELVGGNILRVGK 1035
             E++   Y  D  V   ++++ +       Y  ++   M         +L    I R  +
Sbjct: 875  SEISKLWYFEDEPVP--KSHMDKFTSWYENYRSEMSQAMMETDKVKRNQLTNEVIQRYKQ 934

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038875555.10.0e+0083.68probable RNA-dependent RNA polymerase 1 [Benincasa hispida][more]
KAG6579193.10.0e+0084.32RNA-dependent RNA polymerase 1, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023549735.10.0e+0083.47probable RNA-dependent RNA polymerase 1 [Cucurbita pepo subsp. pepo][more]
XP_022938807.10.0e+0083.38probable RNA-dependent RNA polymerase 1 [Cucurbita moschata][more]
XP_022994004.10.0e+0083.65probable RNA-dependent RNA polymerase 1 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q9LQV20.0e+0057.74RNA-dependent RNA polymerase 1 OS=Arabidopsis thaliana OX=3702 GN=RDR1 PE=2 SV=1[more]
Q0DXS33.0e-26361.41Probable RNA-dependent RNA polymerase 1 OS=Oryza sativa subsp. japonica OX=39947... [more]
O825047.9e-21639.08RNA-dependent RNA polymerase 2 OS=Arabidopsis thaliana OX=3702 GN=RDR2 PE=1 SV=1[more]
Q7XM311.9e-20938.28Probable RNA-dependent RNA polymerase 2 OS=Oryza sativa subsp. japonica OX=39947... [more]
Q9SG024.6e-16337.28RNA-dependent RNA polymerase 6 OS=Arabidopsis thaliana OX=3702 GN=RDR6 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1FF440.0e+0083.38RNA-dependent RNA polymerase OS=Cucurbita moschata OX=3662 GN=LOC111444909 PE=3 ... [more]
A0A6J1K1Q30.0e+0083.65RNA-dependent RNA polymerase OS=Cucurbita maxima OX=3661 GN=LOC111489826 PE=3 SV... [more]
A0A6J1JTB10.0e+0082.74RNA-dependent RNA polymerase OS=Cucurbita maxima OX=3661 GN=LOC111487656 PE=3 SV... [more]
A0A6J1EB470.0e+0082.31RNA-dependent RNA polymerase OS=Cucurbita moschata OX=3662 GN=LOC111430971 PE=3 ... [more]
A0A6J1CZM70.0e+0080.41RNA-dependent RNA polymerase OS=Momordica charantia OX=3673 GN=LOC111015673 PE=3... [more]
Match NameE-valueIdentityDescription
AT1G14790.10.0e+0057.74RNA-dependent RNA polymerase 1 [more]
AT4G11130.15.6e-21739.08RNA-dependent RNA polymerase 2 [more]
AT3G49500.13.2e-16437.28RNA-dependent RNA polymerase 6 [more]
AT2G19930.12.8e-3024.56RNA-dependent RNA polymerase family protein [more]
AT2G19910.12.9e-2424.85RNA-dependent RNA polymerase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR007855RNA-dependent RNA polymerase, eukaryotic-typePFAMPF05183RdRPcoord: 372..933
e-value: 6.0E-183
score: 609.6
IPR007855RNA-dependent RNA polymerase, eukaryotic-typePANTHERPTHR23079RNA-DEPENDENT RNA POLYMERASEcoord: 3..1114
NoneNo IPR availablePANTHERPTHR23079:SF38RNA-DEPENDENT RNA POLYMERASEcoord: 3..1114
NoneNo IPR availableCDDcd00590RRM_SFcoord: 4..65
e-value: 1.81789E-4
score: 38.8253

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg008341.1Spg008341.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0031047 gene silencing by RNA
biological_process GO:0001172 transcription, RNA-templated
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0003723 RNA binding
molecular_function GO:0003968 RNA-directed 5'-3' RNA polymerase activity