Spg008020 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg008020
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	RNase III domain-containing protein
Location	scaffold4: 6486863 .. 6490396 (+)
RNA-Seq Expression	Spg008020
Synteny	Spg008020

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATTTTGCCCTTTGCCCTTTGTAGCCACAAAGAAAGAAGAGAGAAAAAAGAAAAAAGAAGAAAAAAATCCTCTATCGACTTCCAAAAGCCAACGGAATTAAATTAAATTATGTATCACAATCCCCAAATTTTCACCATGGAAGAAGAAGAACACGGAGTAGATTCGTTCGGCGGCGCCGGCCAGGCAGTCAACGGCGAGGTACGGCAGAATCCGGCGGAGATGAGGACCGAGTCGTCTTCGTCGCCGCCGCCGAGTCTGGAGGGAATCGAGGCGATTCTGAATTATGAGTTTCGAGATAAGAGATTGCTGGAAGAGGCTTTCACAGACGCGTCGTGTTCACCGGAAAATTGCTCGTCGTTCGAGCGATTGGAGTACGTCGGCGATTCGGTGTTGAATTTTCTGATGACGAGAGAGCAATACTTCTCGAATCCGAATCTGTCGCCTGGTTCACTGACTCGGCTCCGAGCTGCAAATGTCGACACCGAAAAGCTTGCTCGTGTTGCGATTACCCATGGATTTCATCGGTATCTTCGCCACAACAAGGCTGATCTTCATACAAAAGTAAGTAATTTTCATTTTAATTTTAATTTCGTTGTTGTGATCGACTCATTAACTTCAGAGGGCTATTAGACTAAAAAATGAAAGTTTGATTTATTAGATATTTTTAAAGTTAACAATTTTTTAAAGTTCAAAAACTACAAATTTAGGTCTCGTTTGATAACCATTTCGTTTTTTGGTTTTTGAAAATTAAGTCTATAAACATTATTTTTACCCACAAGCTTCTTTGATTTTTATCTCCATCCTTTTCCATGTTTTAAAAAACTAAGCCAAATTTTGAAAAAAAAAAAAAATTATCAAAAACTTGTTTTTTTTTTTAAATTTGGCTAGGAACTCAAATCTCTCCACAATTTTAAAAAATCAAAACTAAAAAACGAAATGGTTATCAAACGGGACCTTAGGGACTAAATTTATAATAAAATCTTAATCTTTAACCAATCGATAGGGCCAGTTATGATAATTCTTTTATTTTATTTTTCTAATTTTTAAAAGTTAAGCTCAAAGTTAAACTTAAAATTAATCACGTTTTTTTTTTCTTTGGGTAAAAATATATGAGGAACTTTTTCCATCACCAATTTTTTTTAGTTTCCACTTTTGTCTTTTTTGTTTTCCTTTTTTGGGTAACATATAGAAGGAGCTTTTTCGATTACTTTATTTCTACTTTTGGGAATTTTATAAATAAAATTGGATATCAGTATCATCTCTTCCCTAAAAATTAAAATTACTTTTGAAATTATTGGTTATCAGAAATAATAAGATACTAAAGGACTGGTAAAGAGTTATTCAAAAAAAAAAAAAAAAAAAAAAAAAAGGACTGGTAAAGAATTAAATTAATTAATATTTATTAATATACTTTATGCTTCGAAGTAAATAATAAATTAATTAAGGCATTAAATGTCTTGAAAATTACCTGTAGATTATTTCCATCATCTATTTTATTCTGGTGAAATCTTCAAATCTTTATTTGGTTAAAGATTTTAAAAATAACTTTTAAAAAAAAAATTAAAGCTAATTTCTTATAGAATTTAAGATATAATAACAGATAAATTTAAGAATTTATGTATAACCCAAATAATTAATATTTAAACATATAAGATATATTAAAAAATAAGTTAGGAATTTCTTTAAGACTCAAATTGTGCTCATTTTTCAAAAAAAAAAAAAAAAAAAAAAGACTCAAATTGTGCTCCTTATATTCTCTATAACTAGGACCCAAAAGTCTATTGTTCTCTTTCGTTAGAAAAAAAAAAATTAAATTCTCTTTTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAAATTCTCTTTATATTCTAGCTGTTTTAGGACTCTCTCATAAATGAATAAAATTTTATTTCTCTTATGAGATAAGATTACTTTTTTTTTTTCTTTTTTGAGTTCAACAACATTTAGGAGTCGAGGATACATGTCAATTATCGCTGAGTTATGTTTACTTGGCTATAAGATTACATTTTAAAATTTAACTTTCTCAATATTATATTATTACTCAACCAAAAAAAAAAAAATCAATTCTAAATGATAAGATAAAGCTAAAAAATTACTATGTAAGTCAACCGTTCTCATTGCAATAAGCTTATAAATAAAATTTTTAAAGAAAGAAAATAATGATGATAATAAAATAAAAATAGAAATTAACAAATTCTTAAAAAAAAAATGACAACATCAATGACGTGTGTATAATATATATTTCTCCCACCGACCTTCCGATCTGACACTTGGTCTAGTCCATGAGCAATAATATTTAATATTTTTTTTTTTTTTCTTTTGTCACAAGTAAATCATCTTCTAGAATTAAAATAGGGTTTTTAAGATAACATTAAAACAGGGTTTAAACACATGGAATATAAAATGTTAAGGTTGAATTATTAGTTTCATGGCTGAGTCTGGTTGGATAATCATTTGATTTTTGAAAATTAAGTTTATAAAATACTAGTTCCACCTATTATTTCATTTGTTTTGTTATTTACATTCTTCCATATTTTTAAAAACTAAATCAAATTTTGAAAACTGAAAAAAGTAGGCCCCGTTTGACAGCGATTTACTTTTTTGTTTTTGATTTTTTTAAAATTAAGTCTATAAACACTACTTTCCTCCACTACTTTCTTTGTTTTGTTATCTACAACTTTTCCACATGTTTAAAAAATCAAGTCAATTTTTTGTTTTTGAAATTTGACTGAAAACTTAAGTGTCTACTTAACAAAATGAAAATCATGGTAAGGAAATTTAGAGAAAACAAGCATAATTTTCAGAAACCAAATGATTATCAAACGAGACCGTAGTTATACACTTTTTAAAGTTCAAAACCCTATATTAAACACAAAAATGAAATTTCGGGACCAAATAAACATAAACTTGAAAGTTTTACTTATTTAAGCTTTGATTATTGATAGAATCTATTTGTGACATGACAGATTGAAGAGTTTGCTCAAAGCATAGAGGCATATCCTTTGCATTCAAATGGGATGATCAAAGCTCCAAAAATTCTAGCCGACATTGTTGAATCAACCATTGGAGCTGTTTTTGCCGATAGCAACTCCATCGACACTGTTTGGACGGTAATTAAACTTTTTGAACAAAATTTCCATTCTTAATCATAAATAATCATCACTTGTATTACATTATTTTGATGAATTAAACATGATTTATTTTGTAGATATTTAAAGAGCTATTGGAGCCATTGATTACTCTGAACACTATTAAAAGACATCCAATGACAGAATTGACTGAAATTTGTCAAAAAAGAAGTCTCAAGTTGGAATTCGTGGATCTATGGGAAGAAACAAAAGAAATTCAAGTTTTTATAGACAAAAAACTTGTGGGAAAAGGAAGCTATAGGAAAAAACTTATAGCTCAAAATAGAGCTGCAAAAGATGCTTTGAACAACTTAGATAGAGTTCTTGGAGAAATTAATCAACCTTGTCAACCTATGTAAACTTTACATATATACTAATTCCC

mRNA sequence

ATGTATCACAATCCCCAAATTTTCACCATGGAAGAAGAAGAACACGGAGTAGATTCGTTCGGCGGCGCCGGCCAGGCAGTCAACGGCGAGGTACGGCAGAATCCGGCGGAGATGAGGACCGAGTCGTCTTCGTCGCCGCCGCCGAGTCTGGAGGGAATCGAGGCGATTCTGAATTATGAGTTTCGAGATAAGAGATTGCTGGAAGAGGCTTTCACAGACGCGTCGTGTTCACCGGAAAATTGCTCGTCGTTCGAGCGATTGGAGTACGTCGGCGATTCGGTGTTGAATTTTCTGATGACGAGAGAGCAATACTTCTCGAATCCGAATCTGTCGCCTGGTTCACTGACTCGGCTCCGAGCTGCAAATGTCGACACCGAAAAGCTTGCTCGTGTTGCGATTACCCATGGATTTCATCGGTATCTTCGCCACAACAAGGCTGATCTTCATACAAAAATTGAAGAGTTTGCTCAAAGCATAGAGGCATATCCTTTGCATTCAAATGGGATGATCAAAGCTCCAAAAATTCTAGCCGACATTGTTGAATCAACCATTGGAGCTGTTTTTGCCGATAGCAACTCCATCGACACTGTTTGGACGATATTTAAAGAGCTATTGGAGCCATTGATTACTCTGAACACTATTAAAAGACATCCAATGACAGAATTGACTGAAATTTGTCAAAAAAGAAGTCTCAAGTTGGAATTCGTGGATCTATGGGAAGAAACAAAAGAAATTCAAGTTTTTATAGACAAAAAACTTGTGGGAAAAGGAAGCTATAGGAAAAAACTTATAGCTCAAAATAGAGCTGCAAAAGATGCTTTGAACAACTTAGATAGAGTTCTTGGAGAAATTAATCAACCTTGTCAACCTATGTAA

Coding sequence (CDS)

ATGTATCACAATCCCCAAATTTTCACCATGGAAGAAGAAGAACACGGAGTAGATTCGTTCGGCGGCGCCGGCCAGGCAGTCAACGGCGAGGTACGGCAGAATCCGGCGGAGATGAGGACCGAGTCGTCTTCGTCGCCGCCGCCGAGTCTGGAGGGAATCGAGGCGATTCTGAATTATGAGTTTCGAGATAAGAGATTGCTGGAAGAGGCTTTCACAGACGCGTCGTGTTCACCGGAAAATTGCTCGTCGTTCGAGCGATTGGAGTACGTCGGCGATTCGGTGTTGAATTTTCTGATGACGAGAGAGCAATACTTCTCGAATCCGAATCTGTCGCCTGGTTCACTGACTCGGCTCCGAGCTGCAAATGTCGACACCGAAAAGCTTGCTCGTGTTGCGATTACCCATGGATTTCATCGGTATCTTCGCCACAACAAGGCTGATCTTCATACAAAAATTGAAGAGTTTGCTCAAAGCATAGAGGCATATCCTTTGCATTCAAATGGGATGATCAAAGCTCCAAAAATTCTAGCCGACATTGTTGAATCAACCATTGGAGCTGTTTTTGCCGATAGCAACTCCATCGACACTGTTTGGACGATATTTAAAGAGCTATTGGAGCCATTGATTACTCTGAACACTATTAAAAGACATCCAATGACAGAATTGACTGAAATTTGTCAAAAAAGAAGTCTCAAGTTGGAATTCGTGGATCTATGGGAAGAAACAAAAGAAATTCAAGTTTTTATAGACAAAAAACTTGTGGGAAAAGGAAGCTATAGGAAAAAACTTATAGCTCAAAATAGAGCTGCAAAAGATGCTTTGAACAACTTAGATAGAGTTCTTGGAGAAATTAATCAACCTTGTCAACCTATGTAA

Protein sequence

MYHNPQIFTMEEEEHGVDSFGGAGQAVNGEVRQNPAEMRTESSSSPPPSLEGIEAILNYEFRDKRLLEEAFTDASCSPENCSSFERLEYVGDSVLNFLMTREQYFSNPNLSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNKADLHTKIEEFAQSIEAYPLHSNGMIKAPKILADIVESTIGAVFADSNSIDTVWTIFKELLEPLITLNTIKRHPMTELTEICQKRSLKLEFVDLWEETKEIQVFIDKKLVGKGSYRKKLIAQNRAAKDALNNLDRVLGEINQPCQPM

Homology

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BLAST of Spg008020 vs. NCBI nr
Match: XP_038888348.1 (ribonuclease 3-like protein 3 [Benincasa hispida])

HSP 1 Score: 449.9 bits (1156), Expect = 1.7e-122
Identity = 232/287 (80.84%), Postives = 246/287 (85.71%), Query Frame = 0


Query: 10  MEEEEHGV-----DSFGGAGQAVNGEVRQNPAEMRTESSSSPPPSLEGIEAILNYEFRDK 69
           MEEEE  V     +   G G+AVNG                   SL+GIEAILNYEFRDK
Sbjct: 1   MEEEEQRVVFLEEEGSSGGGEAVNG--------------GDSAASLDGIEAILNYEFRDK 60

Query: 70  RLLEEAFTDASCSPENCSSFERLEYVGDSVLNFLMTREQYFSNPNLSPGSLTRLRAANVD 129
           RLLEEAFTDAS SPENCSSFERLEYVGDSVLNFL+TREQYFSNPNLSPGSLTRLRAANVD
Sbjct: 61  RLLEEAFTDASYSPENCSSFERLEYVGDSVLNFLITREQYFSNPNLSPGSLTRLRAANVD 120

Query: 130 TEKLARVAITHGFHRYLRHNKADLHTKIEEFAQSIEAYPLHSNGMIKAPKILADIVESTI 189
           TEKLARVAITHGFHRYLRHNK DLH KIEEF +SIE YPLHSNGMIKAPK+LADIVESTI
Sbjct: 121 TEKLARVAITHGFHRYLRHNKPDLHPKIEEFIKSIEDYPLHSNGMIKAPKVLADIVESTI 180

Query: 190 GAVFADSNSIDTVWTIFKELLEPLITLNTIKRHPMTELTEICQKRSLKLEFVDLWEETKE 249
           GAVFADSNSI+TVW IFK+L+EPLITL T+K+HPMTELTEICQKR+L LEFVD WEE KE
Sbjct: 181 GAVFADSNSIETVWKIFKDLMEPLITLKTMKKHPMTELTEICQKRNLNLEFVDCWEERKE 240

Query: 250 IQVFIDKKLVGKGSYRKKLIAQNRAAKDALNNLDRVLGEINQPCQPM 292
           +QVFIDK+LVGKGSYRKKLIAQNRAAKDALNNLDRVLGE NQPCQPM
Sbjct: 241 VQVFIDKQLVGKGSYRKKLIAQNRAAKDALNNLDRVLGEFNQPCQPM 273

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BLAST of Spg008020 vs. NCBI nr
Match: XP_022136054.1 (ribonuclease 3-like protein 3 [Momordica charantia])

HSP 1 Score: 448.7 bits (1153), Expect = 3.7e-122
Identity = 229/284 (80.63%), Postives = 250/284 (88.03%), Query Frame = 0


Query: 13  EEHGVDSF-----GGAGQAVNGEVRQNPAEMRTESSSSPPPSLEGIEAILNYEFRDKRLL 72
           EEHGV+ F        G  VN +VR+NP E     SS  PPSLE +EAIL YEFRDKRLL
Sbjct: 2   EEHGVELFEQLRLQAQGAVVNDDVRRNPPE-----SSESPPSLEEVEAILGYEFRDKRLL 61

Query: 73  EEAFTDASCSPENCSSFERLEYVGDSVLNFLMTREQYFSNPNLSPGSLTRLRAANVDTEK 132
           EEAFTD+S SPENC SFERLEYVGDSVLNFL+TREQYFSNP+LSPGSLTRLRAANVDTEK
Sbjct: 62  EEAFTDSSYSPENCFSFERLEYVGDSVLNFLITREQYFSNPSLSPGSLTRLRAANVDTEK 121

Query: 133 LARVAITHGFHRYLRHNKADLHTKIEEFAQSIEAYPLHSNGMIKAPKILADIVESTIGAV 192
           LARVAITHGFHRYLRHNK+DL  KIEEFA+ ++ YPLHSNG+IKAPKILADIVESTIGAV
Sbjct: 122 LARVAITHGFHRYLRHNKSDLDKKIEEFAEGMKEYPLHSNGLIKAPKILADIVESTIGAV 181

Query: 193 FADSNSIDTVWTIFKELLEPLITLNTIKRHPMTELTEICQKRSLKLEFVDLWEETKEIQV 252
           F DSNS+DTVWTIF+ L+EPLITL TIKRHPMTELTEICQKR+LKLEFVD WE+TKE+QV
Sbjct: 182 FTDSNSMDTVWTIFRGLMEPLITLKTIKRHPMTELTEICQKRNLKLEFVDSWEKTKEVQV 241

Query: 253 FIDKKLVGKGSYRKKLIAQNRAAKDALNNLDRVLGEINQPCQPM 292
           FIDK+LVGKGSYRKKLIAQNRAAKDALNNLD +L +INQPCQPM
Sbjct: 242 FIDKQLVGKGSYRKKLIAQNRAAKDALNNLDTLLVQINQPCQPM 280

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BLAST of Spg008020 vs. NCBI nr
Match: XP_004144598.1 (ribonuclease 3-like protein 3 [Cucumis sativus] >KGN43500.1 hypothetical protein Csa_020414 [Cucumis sativus])

HSP 1 Score: 438.3 bits (1126), Expect = 5.0e-119
Identity = 228/289 (78.89%), Postives = 244/289 (84.43%), Query Frame = 0


Query: 10  MEEEEHGVD-------SFGGAGQAVNGEVRQNPAEMRTESSSSPPPSLEGIEAILNYEFR 69
           MEE +  VD       S GG G+AVNG              S  P SL+GIEAILNYEFR
Sbjct: 1   MEEGKKRVDFLEGKDGSRGGGGEAVNG--------------SDSPASLDGIEAILNYEFR 60

Query: 70  DKRLLEEAFTDASCSPENCSSFERLEYVGDSVLNFLMTREQYFSNPNLSPGSLTRLRAAN 129
           DKRLLEEAFTDAS SPENCSSFERLEYVGDSVLNFL+TREQYFSNPNLSPGSLTRLRAAN
Sbjct: 61  DKRLLEEAFTDASYSPENCSSFERLEYVGDSVLNFLITREQYFSNPNLSPGSLTRLRAAN 120

Query: 130 VDTEKLARVAITHGFHRYLRHNKADLHTKIEEFAQSIEAYPLHSNGMIKAPKILADIVES 189
           VDTEKLARVAITHGFHRYLRHNKADLH KIEEF +SIE YPLHSNGMIKAPK+LADIVES
Sbjct: 121 VDTEKLARVAITHGFHRYLRHNKADLHQKIEEFGRSIEEYPLHSNGMIKAPKVLADIVES 180

Query: 190 TIGAVFADSNSIDTVWTIFKELLEPLITLNTIKRHPMTELTEICQKRSLKLEFVDLWEET 249
           TIGAVFADSN IDTVW IFKEL+EPLITL T+K+HPMTELTE+CQKRSLKLEF D WEET
Sbjct: 181 TIGAVFADSNFIDTVWKIFKELMEPLITLKTMKKHPMTELTEMCQKRSLKLEFRDRWEET 240

Query: 250 KEIQVFIDKKLVGKGSYRKKLIAQNRAAKDALNNLDRVLGEINQPCQPM 292
           KEI+V I+K++VGKGSYRKKLIAQNRAAK+AL NLD    + NQPCQPM
Sbjct: 241 KEIEVLIEKQVVGKGSYRKKLIAQNRAAKNALENLDTFFPQFNQPCQPM 275

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BLAST of Spg008020 vs. NCBI nr
Match: XP_023554575.1 (ribonuclease 3-like protein 3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 425.2 bits (1092), Expect = 4.4e-115
Identity = 212/255 (83.14%), Postives = 228/255 (89.41%), Query Frame = 0


Query: 37  EMRTESSSSPPPSLEGIEAILNYEFRDKRLLEEAFTDASCSPENCSSFERLEYVGDSVLN 96
           E       S   SL+G+EAILNYEFRDKRLLEEAFTDAS SPENCSSFERLEYVGDSVLN
Sbjct: 12  EQEGRGGESTAASLDGMEAILNYEFRDKRLLEEAFTDASYSPENCSSFERLEYVGDSVLN 71

Query: 97  FLMTREQYFSNPNLSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNKADLHTKIEEFA 156
           FL+TREQYFSNPNLSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNKADL  KIEEF+
Sbjct: 72  FLITREQYFSNPNLSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNKADLDQKIEEFS 131

Query: 157 QSIEAYPLHSNGMIKAPKILADIVESTIGAVFADSNSIDTVWTIFKELLEPLITLNTIKR 216
           ++IE YPLHSNG+IKAPKILADIVES IGAVF DSNSIDTVWTIFK L+EPLITL T+KR
Sbjct: 132 KNIEEYPLHSNGLIKAPKILADIVESCIGAVFTDSNSIDTVWTIFKGLMEPLITLKTMKR 191

Query: 217 HPMTELTEICQKRSLKLEFVDLWEETKEIQVFIDKKLVGKGSYRKKLIAQNRAAKDALNN 276
           HPMTELTE+CQKR+LK EFVDLWEE KEI V IDK+LVGKGSY KKLIAQNRAAKDAL+N
Sbjct: 192 HPMTELTEMCQKRNLKWEFVDLWEERKEIHVLIDKQLVGKGSYGKKLIAQNRAAKDALDN 251

Query: 277 LDRVLGEINQPCQPM 292
              +LG+ N+PCQPM
Sbjct: 252 FHTILGQFNEPCQPM 266

[Close]

BLAST of Spg008020 vs. NCBI nr
Match: XP_022969213.1 (ribonuclease 3-like protein 3 [Cucurbita maxima])

HSP 1 Score: 425.2 bits (1092), Expect = 4.4e-115
Identity = 212/247 (85.83%), Postives = 227/247 (91.90%), Query Frame = 0


Query: 45  SPPPSLEGIEAILNYEFRDKRLLEEAFTDASCSPENCSSFERLEYVGDSVLNFLMTREQY 104
           S   SL+G+EAILNYEFRDKRLLEEAFTDAS SPENCSSFERLEYVGDSVLNFL+TREQY
Sbjct: 20  STAASLDGMEAILNYEFRDKRLLEEAFTDASYSPENCSSFERLEYVGDSVLNFLITREQY 79

Query: 105 FSNPNLSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNKADLHTKIEEFAQSIEAYPL 164
           FSNPNLSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNKADL  KIEEF+++IE YPL
Sbjct: 80  FSNPNLSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNKADLDQKIEEFSKNIEEYPL 139

Query: 165 HSNGMIKAPKILADIVESTIGAVFADSNSIDTVWTIFKELLEPLITLNTIKRHPMTELTE 224
           HSNGMIKAPKILADIVES IGAVF DSNSIDTVWTIFK L+EPLITL T+KRHPMTELTE
Sbjct: 140 HSNGMIKAPKILADIVESCIGAVFTDSNSIDTVWTIFKGLMEPLITLKTMKRHPMTELTE 199

Query: 225 ICQKRSLKLEFVDLWEETKEIQVFIDKKLVGKGSYRKKLIAQNRAAKDALNNLDRVLGEI 284
           +CQKR+LK EFVDLWEE KEI V IDK+LVGKGSY KKLIAQNRAAKDAL+N   +LG+ 
Sbjct: 200 MCQKRNLKWEFVDLWEERKEIHVLIDKQLVGKGSYGKKLIAQNRAAKDALDNFHTILGQF 259

Query: 285 NQPCQPM 292
           N+PCQPM
Sbjct: 260 NEPCQPM 266

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BLAST of Spg008020 vs. ExPASy Swiss-Prot
Match: Q69KJ0 (Ribonuclease 3-like protein 3 OS=Oryza sativa subsp. japonica OX=39947 GN=Os06g0358800 PE=2 SV=1)

HSP 1 Score: 224.9 bits (572), Expect = 1.1e-57
Identity = 115/242 (47.52%), Postives = 167/242 (69.01%), Query Frame = 0


Query: 47  PPSLEGIEAILNYEFRDKRLLEEAFTDAS--CSPENCSSFERLEYVGDSVLNFLMTREQY 106
           P  +  +E++L YEFRDK L+EEA T  S         ++ERLEY+GD+VL  +++RE +
Sbjct: 36  PGEVAAVESLLGYEFRDKALVEEALTHGSFYYPYRPGVTYERLEYLGDAVLTCVVSREVF 95

Query: 107 FSNPNLSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNKADLHTKIEEFAQSIEAYPL 166
            +   L PG LTRLRAANVD EKLARVA+ HG H +LRH   +L  +I +F + +  YP+
Sbjct: 96  LTYGQLQPGPLTRLRAANVDKEKLARVAVVHGLHHFLRHKAPNLDGQITDFIEELSMYPI 155

Query: 167 HSNGMIKAPKILADIVESTIGAVFADSN-SIDTVWTIFKELLEPLITLNTIKRHPMTELT 226
           HSNG++  PK+L D+VES IGA++ DSN + + VW +F++L +PLI+L T+ +HP++EL 
Sbjct: 156 HSNGLLDPPKVLCDVVESLIGAIYCDSNFNQEIVWQVFQKLADPLISLETLGKHPVSELF 215

Query: 227 EICQKRSLKLEFV-DLWEETKEIQVFIDKKLVGKGSY-RKKLIAQNRAAKDALNNLDRVL 284
           E CQK    ++ V D W++   ++V ID ++VG+ +Y +KK IAQNRAAK AL+ L   L
Sbjct: 216 EFCQKTRRGVKIVKDEWDKNLTVEVLIDGEMVGRATYAQKKEIAQNRAAKAALDKLKETL 275

[Close]

BLAST of Spg008020 vs. ExPASy Swiss-Prot
Match: Q9LTQ0 (Ribonuclease 3-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=RTL2 PE=1 SV=1)

HSP 1 Score: 174.1 bits (440), Expect = 2.3e-42
Identity = 96/244 (39.34%), Postives = 153/244 (62.70%), Query Frame = 0


Query: 44  SSPPPSLEGIEAILNYEFRDKRLLEEAFTDASCSPENCSSFERLEYVGDSVLNFLMTREQ 103
           SS   S+E +E ILNY+F +K LL+EA T  SC+  +  S+ERLE++GDS +   ++   
Sbjct: 54  SSEMESMEAVEKILNYKFSNKSLLKEAITHTSCT--DFPSYERLEFIGDSAIGLAISNYL 113

Query: 104 YFSNPNLSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNKADLHTKIEEFAQSI---E 163
           Y + P+L P  L+ LRAANV TEKLARV++ HG + +LR N   L  K++EF++++   +
Sbjct: 114 YLTYPSLEPHDLSLLRAANVSTEKLARVSLNHGLYSFLRRNAPSLDEKVKEFSEAVGKED 173

Query: 164 AYPLHSNGMIKAPKILADIVESTIGAVFADSN-SIDTVWTIFKELLEPLITLNTIKR--H 223
              +   G++KAPK+LAD+ ES  GAV+ D N  +  +W IF+ LLEP++TL+ +++   
Sbjct: 174 DLSVSYGGLVKAPKVLADLFESLAGAVYVDVNFDLQRLWVIFRGLLEPIVTLDDLQKQPQ 233

Query: 224 PMTELTEICQKRSLKLEFVDLWEETKEIQV-FIDKKLVGKGSYRKKLIAQNRAAKDALNN 281
           P++ L ++C K   +++  +  +    I V ++D +L+  G    K IA+  AAK+AL  
Sbjct: 234 PVSMLFKLCHKHKKRIDIKNWKDGNVSIAVIYLDDELLASGRAENKDIARLIAAKEALRK 293

[Close]

BLAST of Spg008020 vs. ExPASy Swiss-Prot
Match: Q9SP32 (Endoribonuclease Dicer homolog 1 OS=Arabidopsis thaliana OX=3702 GN=DCL1 PE=1 SV=2)

HSP 1 Score: 172.9 bits (437), Expect = 5.2e-42
Identity = 99/231 (42.86%), Postives = 138/231 (59.74%), Query Frame = 0


Query: 52   GIEAILNYEFRDKRLLEEAFTDASCSPENCSSFERLEYVGDSVLNFLMTREQYFSNPNLS 111
            G+E  L YEF++K LL EA T AS      S ++RLE+VGD+VL+ L+TR  +F+  +L 
Sbjct: 1561 GLERALKYEFKEKGLLVEAITHASRPSSGVSCYQRLEFVGDAVLDHLITRHLFFTYTSLP 1620

Query: 112  PGSLTRLRAANVDTEKLARVAITHGFHRYLRHNKADLHTKIEEFAQSIE------AYPLH 171
            PG LT LRAA V+ E  ARVA+ H  H YLRH  + L  +I EF + ++       +   
Sbjct: 1621 PGRLTDLRAAAVNNENFARVAVKHKLHLYLRHGSSALEKQIREFVKEVQTESSKPGFNSF 1680

Query: 172  SNGMIKAPKILADIVESTIGAVFADSNSIDT-VWTIFKELLEPLITLNTIKRHPMTELTE 231
              G  KAPK+L DIVES  GA+F DS    T  W +F+ LL+P++T  T+  HP+ EL E
Sbjct: 1681 GLGDCKAPKVLGDIVESIAGAIFLDSGKDTTAAWKVFQPLLQPMVTPETLPMHPVRELQE 1740

Query: 232  ICQKRSLKLEF-VDLWEETKEIQVFIDKKLVGKGSYRKKLIAQNRAAKDAL 275
             CQ+++  LE+       T  ++VFID   VG     +K +AQ  AA++AL
Sbjct: 1741 RCQQQAEGLEYKASRSGNTATVEVFIDGVQVGVAQNPQKKMAQKLAARNAL 1791

[Close]

BLAST of Spg008020 vs. ExPASy Swiss-Prot
Match: Q6ATG6 (Ribonuclease 3-like protein 2 OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0271300 PE=2 SV=2)

HSP 1 Score: 166.8 bits (421), Expect = 3.7e-40
Identity = 95/235 (40.43%), Postives = 140/235 (59.57%), Query Frame = 0


Query: 53  IEAILNYEFRDKRLLEEAFTDASCSPENCSSFERLEYVGDSVLNFLMTREQYFSNPNLSP 112
           +E +L Y+FRD RLLEEA T  S + ++  S++RLE+VGDS L    +   Y +NP L P
Sbjct: 40  VERLLRYQFRDGRLLEEALTHQSFA-DDAVSYQRLEFVGDSALGLAFSNFLYLTNPTLGP 99

Query: 113 GSLTRLRAANVDTEKLARVAITHGFHRYLRHNKADLHTKIEEFAQSIEAYP-------LH 172
           G L+ LRAAN+ TEKLARVA+ H  +  LR N   L   + +F ++++  P        +
Sbjct: 100 GPLSTLRAANISTEKLARVAVRHDLYPLLRRNCPRLDLLVGQFIETVKQEPEDDLSTVPY 159

Query: 173 SNGMIKAPKILADIVESTIGAVFADSN-SIDTVWTIFKELLEPLITLNTIKRHPMTELTE 232
              ++KAPK+LADIVE+   AV+ D    ++ +W + + L EP+IT  TI   P+T L E
Sbjct: 160 GGSVVKAPKVLADIVEAIAAAVYVDCKFDLEKLWKVTRWLFEPIITAETIDEQPVTMLHE 219

Query: 233 ICQKRSLKLEFVDLWEE--TKEIQVFIDKKLVGKGSYRKKLIAQNRAAKDALNNL 278
           +CQK     +F   W++     + VF+  +LVG GS  +K+IA+  AA+DA   L
Sbjct: 220 LCQKHGKMAQF-KTWQKGGMTVVNVFVAGELVGIGSSEQKVIAKLNAARDATRKL 272

[Close]

BLAST of Spg008020 vs. ExPASy Swiss-Prot
Match: Q8LMR2 (Endoribonuclease Dicer homolog 1 OS=Oryza sativa subsp. japonica OX=39947 GN=DCL1 PE=3 SV=1)

HSP 1 Score: 164.9 bits (416), Expect = 1.4e-39
Identity = 94/234 (40.17%), Postives = 138/234 (58.97%), Query Frame = 0


Query: 49   SLEGIEAILNYEFRDKRLLEEAFTDASCSPENCSSFERLEYVGDSVLNFLMTREQYFSNP 108
            + + ++ +L  EF++K LL EA T AS      S ++RLE+VGD+VL+ L+TR  +F+  
Sbjct: 1537 NFDTLKGVLGIEFQNKGLLVEAITHASRPSSGVSCYQRLEFVGDAVLDHLITRHLFFTYT 1596

Query: 109  NLSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNKADLHTKIEEFAQSIEAYPLHSN- 168
            +L PG LT LRAA V+ E  ARVA+ H  H +LRH  + L T+I EF + ++   L    
Sbjct: 1597 DLPPGRLTDLRAAAVNNENFARVAVKHKLHVHLRHGSSALETQIREFVKDVQEELLKPGF 1656

Query: 169  -----GMIKAPKILADIVESTIGAVFADSN-SIDTVWTIFKELLEPLITLNTIKRHPMTE 228
                 G  KAPK+L DIVES  GA+F DS      VW +F+ LL P++T  T+  HP+ E
Sbjct: 1657 NSFGLGDCKAPKVLGDIVESIAGAIFLDSGYDTSVVWKVFQPLLHPMVTPETLPMHPVRE 1716

Query: 229  LTEICQKRSLKLEF-VDLWEETKEIQVFIDKKLVGKGSYRKKLIAQNRAAKDAL 275
            L E CQ+++  LE+          ++VF+D   +G     +K +AQ  AA++AL
Sbjct: 1717 LQERCQQQAEGLEYKASRAGNIATVEVFVDGVQIGVAQNPQKKMAQKLAARNAL 1770

[Close]

BLAST of Spg008020 vs. ExPASy TrEMBL
Match: A0A6J1C375 (ribonuclease 3-like protein 3 OS=Momordica charantia OX=3673 GN=LOC111007843 PE=4 SV=1)

HSP 1 Score: 448.7 bits (1153), Expect = 1.8e-122
Identity = 229/284 (80.63%), Postives = 250/284 (88.03%), Query Frame = 0


Query: 13  EEHGVDSF-----GGAGQAVNGEVRQNPAEMRTESSSSPPPSLEGIEAILNYEFRDKRLL 72
           EEHGV+ F        G  VN +VR+NP E     SS  PPSLE +EAIL YEFRDKRLL
Sbjct: 2   EEHGVELFEQLRLQAQGAVVNDDVRRNPPE-----SSESPPSLEEVEAILGYEFRDKRLL 61

Query: 73  EEAFTDASCSPENCSSFERLEYVGDSVLNFLMTREQYFSNPNLSPGSLTRLRAANVDTEK 132
           EEAFTD+S SPENC SFERLEYVGDSVLNFL+TREQYFSNP+LSPGSLTRLRAANVDTEK
Sbjct: 62  EEAFTDSSYSPENCFSFERLEYVGDSVLNFLITREQYFSNPSLSPGSLTRLRAANVDTEK 121

Query: 133 LARVAITHGFHRYLRHNKADLHTKIEEFAQSIEAYPLHSNGMIKAPKILADIVESTIGAV 192
           LARVAITHGFHRYLRHNK+DL  KIEEFA+ ++ YPLHSNG+IKAPKILADIVESTIGAV
Sbjct: 122 LARVAITHGFHRYLRHNKSDLDKKIEEFAEGMKEYPLHSNGLIKAPKILADIVESTIGAV 181

Query: 193 FADSNSIDTVWTIFKELLEPLITLNTIKRHPMTELTEICQKRSLKLEFVDLWEETKEIQV 252
           F DSNS+DTVWTIF+ L+EPLITL TIKRHPMTELTEICQKR+LKLEFVD WE+TKE+QV
Sbjct: 182 FTDSNSMDTVWTIFRGLMEPLITLKTIKRHPMTELTEICQKRNLKLEFVDSWEKTKEVQV 241

Query: 253 FIDKKLVGKGSYRKKLIAQNRAAKDALNNLDRVLGEINQPCQPM 292
           FIDK+LVGKGSYRKKLIAQNRAAKDALNNLD +L +INQPCQPM
Sbjct: 242 FIDKQLVGKGSYRKKLIAQNRAAKDALNNLDTLLVQINQPCQPM 280

[Close]

BLAST of Spg008020 vs. ExPASy TrEMBL
Match: A0A0A0K1J8 (RNase III domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G041940 PE=4 SV=1)

HSP 1 Score: 438.3 bits (1126), Expect = 2.4e-119
Identity = 228/289 (78.89%), Postives = 244/289 (84.43%), Query Frame = 0


Query: 10  MEEEEHGVD-------SFGGAGQAVNGEVRQNPAEMRTESSSSPPPSLEGIEAILNYEFR 69
           MEE +  VD       S GG G+AVNG              S  P SL+GIEAILNYEFR
Sbjct: 1   MEEGKKRVDFLEGKDGSRGGGGEAVNG--------------SDSPASLDGIEAILNYEFR 60

Query: 70  DKRLLEEAFTDASCSPENCSSFERLEYVGDSVLNFLMTREQYFSNPNLSPGSLTRLRAAN 129
           DKRLLEEAFTDAS SPENCSSFERLEYVGDSVLNFL+TREQYFSNPNLSPGSLTRLRAAN
Sbjct: 61  DKRLLEEAFTDASYSPENCSSFERLEYVGDSVLNFLITREQYFSNPNLSPGSLTRLRAAN 120

Query: 130 VDTEKLARVAITHGFHRYLRHNKADLHTKIEEFAQSIEAYPLHSNGMIKAPKILADIVES 189
           VDTEKLARVAITHGFHRYLRHNKADLH KIEEF +SIE YPLHSNGMIKAPK+LADIVES
Sbjct: 121 VDTEKLARVAITHGFHRYLRHNKADLHQKIEEFGRSIEEYPLHSNGMIKAPKVLADIVES 180

Query: 190 TIGAVFADSNSIDTVWTIFKELLEPLITLNTIKRHPMTELTEICQKRSLKLEFVDLWEET 249
           TIGAVFADSN IDTVW IFKEL+EPLITL T+K+HPMTELTE+CQKRSLKLEF D WEET
Sbjct: 181 TIGAVFADSNFIDTVWKIFKELMEPLITLKTMKKHPMTELTEMCQKRSLKLEFRDRWEET 240

Query: 250 KEIQVFIDKKLVGKGSYRKKLIAQNRAAKDALNNLDRVLGEINQPCQPM 292
           KEI+V I+K++VGKGSYRKKLIAQNRAAK+AL NLD    + NQPCQPM
Sbjct: 241 KEIEVLIEKQVVGKGSYRKKLIAQNRAAKNALENLDTFFPQFNQPCQPM 275

[Close]

BLAST of Spg008020 vs. ExPASy TrEMBL
Match: A0A6J1I0C1 (ribonuclease 3-like protein 3 OS=Cucurbita maxima OX=3661 GN=LOC111468279 PE=4 SV=1)

HSP 1 Score: 425.2 bits (1092), Expect = 2.1e-115
Identity = 212/247 (85.83%), Postives = 227/247 (91.90%), Query Frame = 0


Query: 45  SPPPSLEGIEAILNYEFRDKRLLEEAFTDASCSPENCSSFERLEYVGDSVLNFLMTREQY 104
           S   SL+G+EAILNYEFRDKRLLEEAFTDAS SPENCSSFERLEYVGDSVLNFL+TREQY
Sbjct: 20  STAASLDGMEAILNYEFRDKRLLEEAFTDASYSPENCSSFERLEYVGDSVLNFLITREQY 79

Query: 105 FSNPNLSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNKADLHTKIEEFAQSIEAYPL 164
           FSNPNLSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNKADL  KIEEF+++IE YPL
Sbjct: 80  FSNPNLSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNKADLDQKIEEFSKNIEEYPL 139

Query: 165 HSNGMIKAPKILADIVESTIGAVFADSNSIDTVWTIFKELLEPLITLNTIKRHPMTELTE 224
           HSNGMIKAPKILADIVES IGAVF DSNSIDTVWTIFK L+EPLITL T+KRHPMTELTE
Sbjct: 140 HSNGMIKAPKILADIVESCIGAVFTDSNSIDTVWTIFKGLMEPLITLKTMKRHPMTELTE 199

Query: 225 ICQKRSLKLEFVDLWEETKEIQVFIDKKLVGKGSYRKKLIAQNRAAKDALNNLDRVLGEI 284
           +CQKR+LK EFVDLWEE KEI V IDK+LVGKGSY KKLIAQNRAAKDAL+N   +LG+ 
Sbjct: 200 MCQKRNLKWEFVDLWEERKEIHVLIDKQLVGKGSYGKKLIAQNRAAKDALDNFHTILGQF 259

Query: 285 NQPCQPM 292
           N+PCQPM
Sbjct: 260 NEPCQPM 266

[Close]

BLAST of Spg008020 vs. ExPASy TrEMBL
Match: A0A6J1GKQ4 (ribonuclease 3-like protein 3 OS=Cucurbita moschata OX=3662 GN=LOC111455247 PE=4 SV=1)

HSP 1 Score: 420.6 bits (1080), Expect = 5.2e-114
Identity = 209/247 (84.62%), Postives = 226/247 (91.50%), Query Frame = 0


Query: 45  SPPPSLEGIEAILNYEFRDKRLLEEAFTDASCSPENCSSFERLEYVGDSVLNFLMTREQY 104
           S   SL+G+EAIL YEFRDKRLLEEAFTDAS SPENCSSFERLEYVGDSVLNFL+TREQY
Sbjct: 20  STAASLDGMEAILKYEFRDKRLLEEAFTDASYSPENCSSFERLEYVGDSVLNFLITREQY 79

Query: 105 FSNPNLSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNKADLHTKIEEFAQSIEAYPL 164
           FSNPNLSPG+LTRLRAANVDTEKLARVAITHGFHRYLRHNKADL  KIEEF+++IE YPL
Sbjct: 80  FSNPNLSPGTLTRLRAANVDTEKLARVAITHGFHRYLRHNKADLDQKIEEFSKNIEEYPL 139

Query: 165 HSNGMIKAPKILADIVESTIGAVFADSNSIDTVWTIFKELLEPLITLNTIKRHPMTELTE 224
           HSNG+IKAPKILADIVES IGAVF DSNSIDTVWTIFK L+EPLITL T+KRHPMTELTE
Sbjct: 140 HSNGLIKAPKILADIVESCIGAVFTDSNSIDTVWTIFKGLMEPLITLKTMKRHPMTELTE 199

Query: 225 ICQKRSLKLEFVDLWEETKEIQVFIDKKLVGKGSYRKKLIAQNRAAKDALNNLDRVLGEI 284
           +CQKR+LK EFVDLWEE KEI V IDK+LVGKGSY KKLIAQNRAAKDAL+N   +LG+ 
Sbjct: 200 MCQKRNLKWEFVDLWEERKEIHVLIDKQLVGKGSYGKKLIAQNRAAKDALDNFHTILGQF 259

Query: 285 NQPCQPM 292
           N+PCQPM
Sbjct: 260 NEPCQPM 266

[Close]

BLAST of Spg008020 vs. ExPASy TrEMBL
Match: A0A1Q3BNT8 (Ribonuclease_3 domain-containing protein (Fragment) OS=Cephalotus follicularis OX=3775 GN=CFOL_v3_13208 PE=4 SV=1)

HSP 1 Score: 309.7 bits (792), Expect = 1.3e-80
Identity = 157/237 (66.24%), Postives = 192/237 (81.01%), Query Frame = 0


Query: 50  LEGIEAILNYEFRDKRLLEEAFTDAS-CSPENCSSFERLEYVGDSVLNFLMTREQYFSNP 109
           LEG+E IL Y+F +K LLEEAFT AS  SP+ C+S+ERLE+VGDSVLN L T+EQYF  P
Sbjct: 1   LEGVEEILGYKFGNKSLLEEAFTHASYTSPDQCASYERLEFVGDSVLNLLFTKEQYFLYP 60

Query: 110 NLSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNKADLHTKIEEFAQSIEAYPLHSNG 169
           NL PG LTRLRAANVDTEKLARV+I HG HRYL H K  L  +I+EF Q+I  YPLHSNG
Sbjct: 61  NLQPGDLTRLRAANVDTEKLARVSIRHGLHRYLCHKKPLLEQQIQEFTQAILDYPLHSNG 120

Query: 170 MIKAPKILADIVESTIGAVFADSN-SIDTVWTIFKELLEPLITLNTIKRHPMTELTEICQ 229
           ++  PK+LADIVESTIGAVF DSN SID VW +FK LLEP+I++ T+KRHP+TEL E+CQ
Sbjct: 121 LVDPPKVLADIVESTIGAVFIDSNLSIDIVWKVFKGLLEPIISVETLKRHPVTELYEVCQ 180

Query: 230 KRSLKLEFVDLWEETKEIQVFIDKKLVGKGSY-RKKLIAQNRAAKDALNNLDRVLGE 284
           K++LK++FVDLW+E+   +VFID +LVG+ +Y  KK IA NRAAKDALNN+ ++L E
Sbjct: 181 KKNLKVKFVDLWKESTAFEVFIDDQLVGRDTYGTKKEIAHNRAAKDALNNMWKILNE 237

[Close]

BLAST of Spg008020 vs. TAIR 10
Match: AT4G15417.1 (RNAse II-like 1 )

HSP 1 Score: 242.7 bits (618), Expect = 3.8e-64
Identity = 128/249 (51.41%), Postives = 170/249 (68.27%), Query Frame = 0


Query: 39  RTESSSSPPP--SLEGIEAILNYEFRDKRLLEEAFTDASCSPENCSSFERLEYVGDSVLN 98
           +T  S+ P     +E +E ILNY+F+DK LL +AFTDAS   +   S+E LE +GDS+LN
Sbjct: 16  KTIGSADPDQLMEIESLEKILNYKFKDKSLLLKAFTDASYVDDKSESYELLELLGDSILN 75

Query: 99  FLMTREQYFSNPNLSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNKADLHTKIEEFA 158
             +  +     P  +PG LT+LRA NVDTEKLARVA+ H  + YLRH K  L  +I EF 
Sbjct: 76  MGIIYDFIKLYPKEAPGPLTKLRAVNVDTEKLARVAVNHQLYSYLRHKKPLLEEQILEFV 135

Query: 159 QSIEAYPLHSNGMIKAPKILADIVESTIGAVFADSNSIDTVWTIFKELLEPLITLNTIKR 218
           +++E YPLHSNG++K PK+LADIVESTIGA+F D NS +TVW + K LLEP+I L+ +K 
Sbjct: 136 EAMEKYPLHSNGLLKVPKVLADIVESTIGAIFMDCNSTETVWKVIKPLLEPIIHLDKMKS 195

Query: 219 HPMTELTEICQKRSLKLEFVDLWEETKEIQVFIDKKLVGKGSYR-KKLIAQNRAAKDALN 278
           HPMTEL E+CQKR+LKL   D WEE +     I+ K VG G +  KK  A+N AAK+A++
Sbjct: 196 HPMTELNEMCQKRNLKLTSKDTWEENQTYCFHIEDKFVGCGHHPVKKETARNFAAKNAID 255

Query: 279 NLDRVLGEI 285
           N  +  G++
Sbjct: 256 NFAKFFGDL 264

[Close]

BLAST of Spg008020 vs. TAIR 10
Match: AT3G20420.1 (RNAse THREE-like protein 2 )

HSP 1 Score: 174.1 bits (440), Expect = 1.6e-43
Identity = 96/244 (39.34%), Postives = 153/244 (62.70%), Query Frame = 0


Query: 44  SSPPPSLEGIEAILNYEFRDKRLLEEAFTDASCSPENCSSFERLEYVGDSVLNFLMTREQ 103
           SS   S+E +E ILNY+F +K LL+EA T  SC+  +  S+ERLE++GDS +   ++   
Sbjct: 54  SSEMESMEAVEKILNYKFSNKSLLKEAITHTSCT--DFPSYERLEFIGDSAIGLAISNYL 113

Query: 104 YFSNPNLSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNKADLHTKIEEFAQSI---E 163
           Y + P+L P  L+ LRAANV TEKLARV++ HG + +LR N   L  K++EF++++   +
Sbjct: 114 YLTYPSLEPHDLSLLRAANVSTEKLARVSLNHGLYSFLRRNAPSLDEKVKEFSEAVGKED 173

Query: 164 AYPLHSNGMIKAPKILADIVESTIGAVFADSN-SIDTVWTIFKELLEPLITLNTIKR--H 223
              +   G++KAPK+LAD+ ES  GAV+ D N  +  +W IF+ LLEP++TL+ +++   
Sbjct: 174 DLSVSYGGLVKAPKVLADLFESLAGAVYVDVNFDLQRLWVIFRGLLEPIVTLDDLQKQPQ 233

Query: 224 PMTELTEICQKRSLKLEFVDLWEETKEIQV-FIDKKLVGKGSYRKKLIAQNRAAKDALNN 281
           P++ L ++C K   +++  +  +    I V ++D +L+  G    K IA+  AAK+AL  
Sbjct: 234 PVSMLFKLCHKHKKRIDIKNWKDGNVSIAVIYLDDELLASGRAENKDIARLIAAKEALRK 293

[Close]

BLAST of Spg008020 vs. TAIR 10
Match: AT1G01040.1 (dicer-like 1 )

HSP 1 Score: 172.9 bits (437), Expect = 3.7e-43
Identity = 99/231 (42.86%), Postives = 138/231 (59.74%), Query Frame = 0


Query: 52   GIEAILNYEFRDKRLLEEAFTDASCSPENCSSFERLEYVGDSVLNFLMTREQYFSNPNLS 111
            G+E  L YEF++K LL EA T AS      S ++RLE+VGD+VL+ L+TR  +F+  +L 
Sbjct: 1561 GLERALKYEFKEKGLLVEAITHASRPSSGVSCYQRLEFVGDAVLDHLITRHLFFTYTSLP 1620

Query: 112  PGSLTRLRAANVDTEKLARVAITHGFHRYLRHNKADLHTKIEEFAQSIE------AYPLH 171
            PG LT LRAA V+ E  ARVA+ H  H YLRH  + L  +I EF + ++       +   
Sbjct: 1621 PGRLTDLRAAAVNNENFARVAVKHKLHLYLRHGSSALEKQIREFVKEVQTESSKPGFNSF 1680

Query: 172  SNGMIKAPKILADIVESTIGAVFADSNSIDT-VWTIFKELLEPLITLNTIKRHPMTELTE 231
              G  KAPK+L DIVES  GA+F DS    T  W +F+ LL+P++T  T+  HP+ EL E
Sbjct: 1681 GLGDCKAPKVLGDIVESIAGAIFLDSGKDTTAAWKVFQPLLQPMVTPETLPMHPVRELQE 1740

Query: 232  ICQKRSLKLEF-VDLWEETKEIQVFIDKKLVGKGSYRKKLIAQNRAAKDAL 275
             CQ+++  LE+       T  ++VFID   VG     +K +AQ  AA++AL
Sbjct: 1741 RCQQQAEGLEYKASRSGNTATVEVFIDGVQVGVAQNPQKKMAQKLAARNAL 1791

[Close]

BLAST of Spg008020 vs. TAIR 10
Match: AT1G01040.2 (dicer-like 1 )

HSP 1 Score: 172.9 bits (437), Expect = 3.7e-43
Identity = 99/231 (42.86%), Postives = 138/231 (59.74%), Query Frame = 0


Query: 52   GIEAILNYEFRDKRLLEEAFTDASCSPENCSSFERLEYVGDSVLNFLMTREQYFSNPNLS 111
            G+E  L YEF++K LL EA T AS      S ++RLE+VGD+VL+ L+TR  +F+  +L 
Sbjct: 1562 GLERALKYEFKEKGLLVEAITHASRPSSGVSCYQRLEFVGDAVLDHLITRHLFFTYTSLP 1621

Query: 112  PGSLTRLRAANVDTEKLARVAITHGFHRYLRHNKADLHTKIEEFAQSIE------AYPLH 171
            PG LT LRAA V+ E  ARVA+ H  H YLRH  + L  +I EF + ++       +   
Sbjct: 1622 PGRLTDLRAAAVNNENFARVAVKHKLHLYLRHGSSALEKQIREFVKEVQTESSKPGFNSF 1681

Query: 172  SNGMIKAPKILADIVESTIGAVFADSNSIDT-VWTIFKELLEPLITLNTIKRHPMTELTE 231
              G  KAPK+L DIVES  GA+F DS    T  W +F+ LL+P++T  T+  HP+ EL E
Sbjct: 1682 GLGDCKAPKVLGDIVESIAGAIFLDSGKDTTAAWKVFQPLLQPMVTPETLPMHPVRELQE 1741

Query: 232  ICQKRSLKLEF-VDLWEETKEIQVFIDKKLVGKGSYRKKLIAQNRAAKDAL 275
             CQ+++  LE+       T  ++VFID   VG     +K +AQ  AA++AL
Sbjct: 1742 RCQQQAEGLEYKASRSGNTATVEVFIDGVQVGVAQNPQKKMAQKLAARNAL 1792

[Close]

BLAST of Spg008020 vs. TAIR 10
Match: AT3G03300.1 (dicer-like 2 )

HSP 1 Score: 133.7 bits (335), Expect = 2.5e-31
Identity = 86/228 (37.72%), Postives = 125/228 (54.82%), Query Frame = 0


Query: 53   IEAILNYEFRDKRLLEEAFTDAS-CSPENCSSFERLEYVGDSVLNFLMTREQYFSNPNLS 112
            +E++LNY F DK LL EA T  S   PE    ++RLE++GDSVL++L+T+  Y   P LS
Sbjct: 1152 MESLLNYSFEDKSLLVEALTHGSYMMPEIPRCYQRLEFLGDSVLDYLITKHLYDKYPCLS 1211

Query: 113  PGSLTRLRAANVDTEKLARVAITHGFHRYLRHNKADLHTKIEEFAQSIEAYPLHS----N 172
            PG LT +R+A+V+ E  A VA+    H+++ +    LH  I       E   L S     
Sbjct: 1212 PGLLTDMRSASVNNECYALVAVKANLHKHILYASHHLHKHISRTVSEFEQSSLQSTFGWE 1271

Query: 173  GMIKAPKILADIVESTIGAVFADSN-SIDTVWTIFKELLEPLITLNTIKRHPMTELTEIC 232
              I  PK+L D++ES  GA+F DS  + + V+   K LL  +IT  T+K HP+ ELTE+C
Sbjct: 1272 SDISFPKVLGDVIESLAGAIFVDSGYNKEVVFASIKPLLGCMITPETVKLHPVRELTELC 1331

Query: 233  QKRSLKL----EFVDLWEETKEIQVFI-------DKKLVGKGSYRKKL 264
            QK   +L    +F     E K  ++         DKK+  K +Y++ L
Sbjct: 1332 QKWQFELSKAKDFDSFTVEVKAKEMSFAHTAKASDKKMAKKLAYKEVL 1379

The following BLAST results are available for this feature:

BLAST of Spg008020 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038888348.1	1.7e-122	80.84	ribonuclease 3-like protein 3 [Benincasa hispida]	[more]
XP_022136054.1	3.7e-122	80.63	ribonuclease 3-like protein 3 [Momordica charantia]	[more]
XP_004144598.1	5.0e-119	78.89	ribonuclease 3-like protein 3 [Cucumis sativus] >KGN43500.1 hypothetical protein...	[more]
XP_023554575.1	4.4e-115	83.14	ribonuclease 3-like protein 3 [Cucurbita pepo subsp. pepo]	[more]
XP_022969213.1	4.4e-115	85.83	ribonuclease 3-like protein 3 [Cucurbita maxima]	[more]

BLAST of Spg008020 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q69KJ0	1.1e-57	47.52	Ribonuclease 3-like protein 3 OS=Oryza sativa subsp. japonica OX=39947 GN=Os06g0...	[more]
Q9LTQ0	2.3e-42	39.34	Ribonuclease 3-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=RTL2 PE=1 SV=1	[more]
Q9SP32	5.2e-42	42.86	Endoribonuclease Dicer homolog 1 OS=Arabidopsis thaliana OX=3702 GN=DCL1 PE=1 SV...	[more]
Q6ATG6	3.7e-40	40.43	Ribonuclease 3-like protein 2 OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0...	[more]
Q8LMR2	1.4e-39	40.17	Endoribonuclease Dicer homolog 1 OS=Oryza sativa subsp. japonica OX=39947 GN=DCL...	[more]

BLAST of Spg008020 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1C375	1.8e-122	80.63	ribonuclease 3-like protein 3 OS=Momordica charantia OX=3673 GN=LOC111007843 PE=...	[more]
A0A0A0K1J8	2.4e-119	78.89	RNase III domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G041940 P...	[more]
A0A6J1I0C1	2.1e-115	85.83	ribonuclease 3-like protein 3 OS=Cucurbita maxima OX=3661 GN=LOC111468279 PE=4 S...	[more]
A0A6J1GKQ4	5.2e-114	84.62	ribonuclease 3-like protein 3 OS=Cucurbita moschata OX=3662 GN=LOC111455247 PE=4...	[more]
A0A1Q3BNT8	1.3e-80	66.24	Ribonuclease_3 domain-containing protein (Fragment) OS=Cephalotus follicularis O...	[more]

BLAST of Spg008020 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G15417.1	3.8e-64	51.41	RNAse II-like 1	[more]
AT3G20420.1	1.6e-43	39.34	RNAse THREE-like protein 2	[more]
AT1G01040.1	3.7e-43	42.86	dicer-like 1	[more]
AT1G01040.2	3.7e-43	42.86	dicer-like 1	[more]
AT3G03300.1	2.5e-31	37.72	dicer-like 2	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 260..280
None	No IPR available	GENE3D	3.30.160.20		coord: 217..286 e-value: 3.8E-9 score: 38.5
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 35..49
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 13..49
None	No IPR available	PANTHER	PTHR14950:SF54	RNASE II-LIKE 1	coord: 38..282
None	No IPR available	PANTHER	PTHR14950	DICER-RELATED	coord: 38..282
None	No IPR available	SUPERFAMILY	54768	dsRNA-binding domain-like	coord: 174..277
IPR014720	Double-stranded RNA-binding domain	SMART	SM00358	DRBM_3	coord: 218..278 e-value: 0.0068 score: 25.6
IPR014720	Double-stranded RNA-binding domain	PFAM	PF00035	dsrm	coord: 218..277 e-value: 1.4E-5 score: 25.7
IPR000999	Ribonuclease III domain	SMART	SM00535	riboneu5	coord: 65..214 e-value: 3.7E-28 score: 109.6
IPR000999	Ribonuclease III domain	PFAM	PF00636	Ribonuclease_3	coord: 85..191 e-value: 4.4E-21 score: 75.5
IPR000999	Ribonuclease III domain	PROSITE	PS00517	RNASE_3_1	coord: 85..93
IPR000999	Ribonuclease III domain	PROSITE	PS50142	RNASE_3_2	coord: 50..192 score: 33.266743
IPR000999	Ribonuclease III domain	CDD	cd00593	RIBOc	coord: 65..213 e-value: 6.92755E-39 score: 131.196
IPR036389	Ribonuclease III, endonuclease domain superfamily	GENE3D	1.10.1520.10	Ribonuclease III domain	coord: 48..216 e-value: 1.2E-47 score: 164.1
IPR036389	Ribonuclease III, endonuclease domain superfamily	SUPERFAMILY	69065	RNase III domain-like	coord: 49..211

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg008020.1	Spg008020.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0030422	production of siRNA involved in RNA interference
biological_process	GO:0090502	RNA phosphodiester bond hydrolysis, endonucleolytic
biological_process	GO:0006511	ubiquitin-dependent protein catabolic process
biological_process	GO:0006396	RNA processing
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0005634	nucleus
molecular_function	GO:0004525	ribonuclease III activity
molecular_function	GO:0003723	RNA binding